LOCUS       DATMFS010000001         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_232363, whole genome shotgun sequence.
ACCESSION   DATMFS010000001 DATMFS010000000
VERSION     DATMFS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232363"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VNQ33_00005"
     CDS             <1..1469
                     /locus_tag="VNQ33_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWJ96503.1"
                     /translation="ELSGKAFADAVGRGGRVVLTDTNRRRAWDINRVANATSDTLAAS
                     ADIDGGNGSTTTLWPDRPADQTVSQLTGGVRVGASRDGFGLHPYGRPSNAFDGDPSTA
                     WITGGFGTAPGSSVWIEFPKPRLIDQITITPWAGASPSTVSAVRVEVGDKQVTEALVP
                     GQDLTVGIEPSTADRVKVTILEQTDGDNPVGISDIAIDDVTVRNLARAPVTLNRLADQ
                     ADADTRAKLAAMPFDVVFTREQGSYQDPNDDEESQLDRVFEIPGARTFTFAASLGVAE
                     ADEAVVAAARNGDRACHRVAMLDGEWIEARVVSSPAELATGAMRIEGCAPIELASGGH
                     QFRTVYQWRLDTVRLASDGTTEPATADPADRTVTVTDRSATQIDMQVEAAGSGSRYLR
                     LGEAFDERWSLTVDGVDAGPPILVDGYSTGWLIDGGAHRLVATFGPQRAVQVTFLASA
                     AAVVGVTAAALLPAPPLLERRRRRGLDAGARPPAGGAP"
     gene            1466..2011
                     /locus_tag="VNQ33_00010"
     CDS             1466..2011
                     /locus_tag="VNQ33_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96504.1"
                     /translation="MTSPETSPEAVPDPSSGSSSDPVPDPVLVPGPVRPSLRAIGAWL
                     LIIGLAVALDGLVGGLAVVFVAAVLLAGLPTRVIGGLGVLALALAPVAFLVEGVPSAS
                     DVSPALVIRSLLPHHLTFVGLVLVSAFALIDLAPHLRSWASAERRAQDDGPPLGAALG
                     AVVVGAVALGALLACRAVLAT"
     gene            2008..>2912
                     /locus_tag="VNQ33_00015"
     CDS             2008..>2912
                     /locus_tag="VNQ33_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96505.1"
                     /translation="MIRNLLERARSSGLLQGSAWILVGLGIQALLGFVFWLLGARVAT
                     ISELGEATALFTAIQFVNYASGLGLTIALARHATLQNRESDALFGWAVVATAVSSVFF
                     GSVYLAVVDTSATRLVNGSVGSWALFCGFTAATSIALLVDVRLMAARRWGWLVGRVAF
                     AVLVRLPFVGLDVGMDPAMWLYILMLTPLSLVGIGSLLFLGRMGCGGISLNRPRALGP
                     FARYSGVNWVATLASQAPQFVLPLYVAQEVPPSVNASFFLAWTVTSLVFLLPAAISQV
                     LLVEGGKDAQLTEASGVPGSEREREA"
BASE COUNT          330 a         1116 c         1016 g          450 t
ORIGIN      
        1 ccgagctgag cggcaaggcg ttcgcggacg ccgtcggccg cggcggccgg gtggtgctca
       61 ccgacacgaa ccgccgccgg gcgtgggaca tcaaccgggt ggccaacgcc acctccgaca
      121 cgctggcggc gagtgccgac atcgatggcg gcaacgggtc caccaccacc ctctggcccg
      181 accgccccgc cgaccagacc gtgtcccagc tcaccggcgg ggtgcgcgtc ggcgccagcc
      241 gagacggctt cgggctgcac ccctacggcc ggccgtcgaa cgcgttcgac ggcgatccgt
      301 ccaccgcgtg gatcaccggc gggttcggca ccgcgcccgg cagcagcgtc tggatcgagt
      361 tcccgaagcc gcgcctcatc gaccagatca ccatcacccc gtgggcgggc gcgtcgccgt
      421 cgacggtgtc cgcggtgcgc gtcgaggtgg gcgacaagca ggtcaccgag gcgctggtcc
      481 cgggccagga cctgacggtg ggcatcgagc cgagcaccgc cgaccgcgtg aaggtcacga
      541 tcctcgagca gaccgatggc gacaaccccg tcggcatcag cgacatcgcc atcgacgacg
      601 tcaccgtccg caacctcgcc cgggcgccgg tgaccctgaa ccgcctggcg gaccaggccg
      661 atgccgacac ccgggcgaag ctggcggcga tgcccttcga cgtcgtgttc acccgggagc
      721 agggctccta ccaggacccg aacgacgacg aggagtccca gctcgaccgg gtcttcgaga
      781 tccccggtgc ccggaccttc accttcgccg catcgctcgg cgtcgccgag gctgacgaag
      841 ccgtggtcgc cgccgcccgc aacggcgacc gcgcctgcca ccgcgtcgcc atgctcgacg
      901 gcgagtggat cgaggcccgc gtggtctcct ccccggccga gctggccacc ggtgcgatgc
      961 gcatcgaggg ctgcgcgccg atcgagctcg cctccggcgg gcaccagttc cggaccgtgt
     1021 accagtggcg cctcgacacc gtccgcctcg cctccgacgg caccaccgaa ccggcgacag
     1081 cggatcctgc ggaccggacg gtgacggtca ccgaccgctc ggccacccag atcgacatgc
     1141 aggtcgaagc ggcgggctcc ggctcccgct acctccgctt gggcgaggcg ttcgacgagc
     1201 ggtggtcgtt gaccgtcgac ggcgtcgatg ctggaccccc gatcctcgtc gacggctact
     1261 ccacgggctg gctgatcgac ggcggggcgc accgcctggt cgcgacgttc gggccgcagc
     1321 gagcggtgca ggtgacgttc ctggcatcgg ccgcggccgt ggtgggcgtc accgcggccg
     1381 cgctcctgcc ggctccgccg ctgctcgagc gccgccgtcg ccgcgggctc gatgccgggg
     1441 cccggccccc ggccggaggg gcgccgtgac atccccggag acctcccccg aggccgtccc
     1501 cgatccgtcg tcgggctcaa gctcggaccc ggtgccggac ccggtgctgg tgccgggccc
     1561 cgtccgcccg tcgctgcggg cgatcggcgc ctggctcctc atcatcggcc tggccgtcgc
     1621 gctcgacggc ctcgtgggag gcctcgcggt ggtgttcgtc gccgccgtgc tgctcgccgg
     1681 cctgcccacc cgcgtgatcg gcgggctcgg ggtcctggcc ctcgctctcg ccccggtcgc
     1741 cttcttggtc gagggcgtgc cgtccgccag cgacgtgtcg ccggccctgg tcatccgctc
     1801 gctcctcccg caccacctca cgttcgtcgg cttggtcctc gtcagcgcgt tcgccctcat
     1861 cgacctggct ccgcacctgc gcagctgggc gtcggccgag cgtcgggccc aagacgacgg
     1921 tcctccgctc ggggccgccc tcggagcggt cgtggtcggg gccgtcgccc tcggggcgct
     1981 gctggcctgc cgggcggtcc tcgccacgtg atccgcaacc tgctggagcg ggcgaggtcg
     2041 tcgggactgc tccagggctc ggcatggatc ctcgtcggcc tgggcatcca ggccctgctc
     2101 ggcttcgtgt tctggctgct cggtgcacgg gtcgccacca tctccgagct gggcgaggcg
     2161 accgccctct tcacggcgat ccagttcgtc aactacgcct cgggtctggg cctcacgatc
     2221 gccctcgctc gccacgcgac gctccagaac cgcgagtccg acgccctgtt cggatgggcc
     2281 gtcgtggcca cggcggtgtc gtcggtcttc ttcggctcgg tgtacctcgc cgtggtcgac
     2341 accagcgcga cccggctcgt caacgggtcg gtcggatcgt gggccttgtt ctgcggcttc
     2401 acggcggcca cgtcgatcgc cctgctcgtc gacgtacggc tgatggcggc gcggcgctgg
     2461 gggtggctgg tcggtcgcgt cgccttcgcc gtgctggtgc gcctgccgtt cgtcggcctc
     2521 gacgtcggca tggatcccgc catgtggctg tacatcctga tgctcacgcc gctctcgctc
     2581 gtcggcatcg ggtcgctgct cttcctgggc cggatgggct gcggcgggat cagcctgaac
     2641 cggccgaggg cgctcgggcc gttcgcccgg tactccgggg tgaactgggt ggccaccctg
     2701 gcgagccagg cgccgcagtt cgtcctcccg ctctacgtgg cccaggaggt cccgccgtcg
     2761 gtgaacgcca gcttcttcct cgcctggacc gtcacgtcgc tcgtgttcct gctccccgcc
     2821 gccatctccc aggtcctgct agtcgagggc ggcaaggacg cgcagctgac cgaagcctcc
     2881 ggcgtaccgg gctccgagcg tgagcgcgag gc
//
LOCUS       DATMFS010000002         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_46700, whole genome shotgun sequence.
ACCESSION   DATMFS010000002 DATMFS010000000
VERSION     DATMFS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46700"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..586)
                     /locus_tag="VNQ33_00020"
     CDS             complement(161..586)
                     /locus_tag="VNQ33_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWJ96506.1"
                     /translation="MAAIIEPRRLPVRPSPRPALRLVTAHDGRSVEAPVDLGFGPVHL
                     AGAVAAVVVAVLLALAIGSGAFAALAPAPGGTAAPASAQRADGTAAGTHVTVEAGDTL
                     WSIARRIQPSGDVRSLVDRLTATYGTAPLQVGQEVTVPA"
     gene            795..1427
                     /gene="lexA"
                     /locus_tag="VNQ33_00025"
     CDS             795..1427
                     /gene="lexA"
                     /locus_tag="VNQ33_00025"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWJ96507.1"
                     /translation="MTDQALTARQRQILECIDASMREKGYPPSVREIGETVGLGSPST
                     VHSHLSTLQRLGFLRRDPSKPRAIEVRWDPQSGAAVERRPIRHVPLVGEVAAGTDVLA
                     QENVEEVVPVPADLTGDGDLFMLKVRGDSMIDAGILDGDFVVVRSQPEARNGEIVVAG
                     IPGEEATVKTYTRSGDQVVLLPANERLSPMEFAASEVQIFGRVVTVMRKL"
     gene            complement(1446..1907)
                     /locus_tag="VNQ33_00030"
     CDS             complement(1446..1907)
                     /locus_tag="VNQ33_00030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWJ96508.1"
                     /translation="MAIDVGQPAPGFTLKGQSGADVSLSDFAGDRAVALVFYPFTFTG
                     VCEGELCALRDDLSRYEAAGVQVLAVSCDSKNSQKVWAEQQGYTFPLLSDFWPHGATA
                     QAYGVFNDALGCANRATFLIDRDGVVVDAFATDSLGTPREQARYEAALAKL"
     gene            complement(1980..>2511)
                     /locus_tag="VNQ33_00035"
     CDS             complement(1980..>2511)
                     /locus_tag="VNQ33_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96509.1"
                     /translation="LDPHGDDEHSAALRAERERRILTALDEGAAWRHRKERVLVVARN
                     EVFRDADDRAHRALWQEHGPACLDAVWRQRWREREAEPGWIEARRWSDDELEAASPAD
                     GEGRAAFGQVDWITVEDHTNTASSGTIDRYQHVSVWCGRALATVTLRHDDALDLDGTT
                     VRIALAAYRRLWQLDR"
BASE COUNT          359 a          913 c          897 g          342 t
ORIGIN      
        1 aaccgccgct ggcaggccag gcaggcgcgc cgacgacgga tcagcaggcc gtcttcggac
       61 tggcgagagt cgacgacctt gtcctcgaag gttccacacg aggggcagcg caccgccccg
      121 acgctacctg cacggccgag gtcctctcat ccccggcgcc tcaagcgggg acggtgacct
      181 cctggccgac ctggaggggc gccgtgccgt aggtggcggt gagccggtcg accagcgacc
      241 ggacgtcgcc cgagggctgg atgcgccggg cgatggacca gagcgtgtcg cctgcttcca
      301 ccgtgacgtg cgtgcccgcc gccgtgccgt cagcacgctg cgccgacgcg ggagccgccg
      361 tgccgcccgg cgccggggcc agcgccgcga aggcgccgct gccgatggcg agggcgagga
      421 gcaccgccac cacgacggcg gcgaccgcac cagcgaggtg cacgggaccg aagcccagat
      481 cgaccggcgc ctcgaccgac cgaccgtcgt gggcggtgac gagacggagc gccggccgcg
      541 gcgaggggcg aacggggagc cggcggggtt cgatgattgc agccatggtg aacatgctcc
      601 ccagggggtc tgacagcgaa gggttccgaa cgcccgttcc acgaacgcct gttcggcagt
      661 cgccatggta accacgaacg cccgttcgcc gtcaaggccc aggacgaaca aatgtttgcc
      721 cgaacacctg cgcgatgcta cggtctcggt cgaccacctg ttcgcccggc ccgggcggca
      781 cgcaaggagc ctccatgacc gatcaagcgc tcaccgcacg ccagcgacag atcctcgagt
      841 gcatcgatgc gtcgatgcgg gagaagggct acccgccctc ggtccgcgag atcggcgaga
      901 cggtcggcct gggctcgccc tccaccgtcc actcccacct ctccacgctg cagcgcctcg
      961 gcttcctccg gcgagatccg tccaagcccc gcgccatcga ggtccggtgg gatccccagt
     1021 cgggtgccgc ggtggagcgc cgccccatcc gccacgtccc cctcgtgggc gaggtggcgg
     1081 ccggcaccga cgtgctggca caggagaacg tcgaggaggt cgtaccggtc ccggccgacc
     1141 tcacgggcga cggggacctg ttcatgctga aggtccgggg cgactcgatg atcgacgccg
     1201 ggatcctcga cggcgacttc gtggtcgtgc gctcccagcc cgaggctcgc aacggcgaga
     1261 tcgtggtcgc cggcatcccc ggcgaggagg ccacggtcaa gacctacacg cgcagcggcg
     1321 accaggtcgt gctgctgccg gccaacgagc gcctgtcccc catggagttc gcggcgtctg
     1381 aggtgcagat cttcggccgg gtcgtcacgg tgatgcgcaa gctctgagcg cccgctggct
     1441 cgtgctcaga gcttggcgag cgccgcttcg tagcgcgcct gctcgcgcgg cgtgccgagg
     1501 ctgtcggtgg cgaacgcgtc gaccaccacg ccgtcacggt cgatcaggaa ggtcgcccgg
     1561 ttggcgcagc cgagggcgtc gttgaagacc ccgtaggcct gggcggtcgc cccgtgcggc
     1621 cagaagtccg acagcagcgg gaacgtgtag ccctgctgct ccgcccacac cttctgcgag
     1681 ttcttcgagt cgcaggagac cgccagcact tgcacacccg ccgcttcgta gcggctgagg
     1741 tcgtcacgca gggcgcagag ctcgccctcg cagaccccgg tgaaggtgaa ggggtagaac
     1801 acgagcgcca ccgcccggtc gccggcgaag tcgctgagcg acacgtcggc accggactgg
     1861 cccttgaggg tgaatccggg tgcgggctgt ccgacgtcga tggccatggc gtgctccttg
     1921 ggtgaggtga ccgcggaagt gtacggacgg gcggccccgg cgtctcggtc gccgcccgat
     1981 cagcggtcga gctgccagag gcgccggtag gccgcgaggg cgatgcgcac cgtggtcccg
     2041 tcgaggtcga gcgcatcgtc gtggcgcagg gtcaccgtgg cgagcgcccg cccgcaccag
     2101 acgctcacgt gctggtaccg gtcgatcgtc cccgacgatg ccgtgttcgt gtggtcctcc
     2161 accgtgatcc agtcgacctg gccgaaggcg gctcggccct cgccgtcggc tgggctggcc
     2221 gcctcgagct cgtcgtcgga ccaccgccgc gcctcgatcc accccggctc cgcctcgcgc
     2281 tcgcgccacc gctgccgcca caccgcatcg aggcaggccg ggccgtgctc ctgccacagc
     2341 gcgcggtgcg cgcggtcgtc ggcgtctcgg aacacctcgt tgcgagccac caccagcacc
     2401 cgctccttgc ggtggcgcca ggcggcaccc tcgtcgagcg cggtgaggat ccgccgctcg
     2461 cgctcggccc gcagcgcggc cgagtgctcg tcgtcgccgt gggggtcgag c
//
LOCUS       DATMFS010000003         7959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_162898, whole genome shotgun sequence.
ACCESSION   DATMFS010000003 DATMFS010000000
VERSION     DATMFS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162898"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VNQ33_00040"
     CDS             <1..682
                     /locus_tag="VNQ33_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96510.1"
                     /translation="AGPISEGSFVFTVWVPVPPRLVPEEDLVLFEIEDEPFERAATLR
                     LHQAMTSLSQAVRQVEDDHAGIEAFTDRLSDGVTAVLCEAVATMVNESRIGLETSFSW
                     ALDRPVADRNQPVVFGRGSQEVLQEAARHIRSLIPEEEIVLAGNVVRLHRDARDGTGE
                     VTIAGVFAGDPDERLRRVTMTLPGAVYDEAIRAHDEFDLVEVVGELYRRGNRLYLRNP
                     RDFLRIER"
     gene            740..2188
                     /locus_tag="VNQ33_00045"
     CDS             740..2188
                     /locus_tag="VNQ33_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HWJ96511.1"
                     /translation="MTADAHSLSPMPEGELVRGDPEQAGCGEPSCTVCGLDHEFDLPE
                     GLIEACHGGEVVIFAGAGISTEVPVAFPNTIFQMAAHRLGMSRETLGTFPAAMQEYER
                     RFGRADLVRLISKKFDYIDSYRDLQFHATRFHSDLATMPYFRDIVTTNWDTYFEDWCG
                     ATPFVTGEDIALWEMPGRRVLKIHGSISNLASLVATDEDYEERLAKLSSDVMGGLLRQ
                     FLATRTVIFVGYSLTDWNFRRLYEALRSDMKGFAPRAYVVSPFPVEENDLDLTVIHTS
                     GVHFLRTIKAQLVGSCFIGDEAYDRVNVVLTKAAEADEVAKTVPHKKYPSVVYCWFYH
                     DGLRDACDRILRLRRTGEYSDRHHVFHLVNSYDEWCDNAYDEARYHDAAYIDGYMNGL
                     MMLLDDDWGREDRLSAYPDMEPLADSIPYYFMYGSDSEMRTDDDLAEALLLSRRRAPK
                     ERKVAREIVGGLAEDMVISHSPFIPDAPGLFT"
     gene            complement(2297..2512)
                     /locus_tag="VNQ33_00050"
     CDS             complement(2297..2512)
                     /locus_tag="VNQ33_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96512.1"
                     /translation="MAHRPNQFQRGETAGIEDASLGLSPCSVDALARQHGDDYARGYT
                     AGYGEKAVAAVRSPGRPWWRSISARLR"
     gene            2578..2811
                     /locus_tag="VNQ33_00055"
     CDS             2578..2811
                     /locus_tag="VNQ33_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ96513.1"
                     /translation="MDKSIYTPDHQRLCLKLRQLRTERGLTQGELAGLLGVNQTFVSK
                     YERGERRLDLLELREVCRALSVSLVEFVREFDI"
     gene            3096..3407
                     /locus_tag="VNQ33_00060"
     CDS             3096..3407
                     /locus_tag="VNQ33_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96514.1"
                     /translation="MDRFPIDGAQLQIHAVEVRPDLRDGNQKTRPDGRLVWAVACLVR
                     FDGRVPEMVTVKVPSATPLKLAEMARVEFGGLYALYWETGGRSGISVQAESVSAVAAA
                     K"
     gene            3440..3817
                     /locus_tag="VNQ33_00065"
     CDS             3440..3817
                     /locus_tag="VNQ33_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96515.1"
                     /translation="MSRAVMAGGPAVAVLGVVGGLPPVVWLLSAACGGLAAWFRHDGR
                     HHPCLAEDVEAALGSGRSLRVEERPLPAGVLLVLIPSPPLTDPQLRAVLAGPVAAHGY
                     KVVSIRQAKRGNLTVTLVELMPC"
     gene            3811..4473
                     /locus_tag="VNQ33_00070"
     CDS             3811..4473
                     /locus_tag="VNQ33_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWJ96516.1"
                     /translation="MLTLDLGVYGPNLGSAAIDLARTTHGLVCGSTGSGKSFLARRLI
                     EQAVASGMDVSVADPKAAGDYTGAADVVADGPEASVALLEHVARALPDRMTEPCDPLL
                     MVVDELSAVQLRVVGETAKEAKDRVDRIHAALGSICLMGRSARIHLLAITQRADTTAI
                     PGWARDQCSWRVAMGWMSPDGLRMMGFGDVAAPTAWPGHGWAVGIVGAPPGQPVPLHV
                     TP"
     gene            4470..5249
                     /locus_tag="VNQ33_00075"
     CDS             4470..5249
                     /locus_tag="VNQ33_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96517.1"
                     /translation="MTAGRTAAPALPLDLDPPCDPRGWTLNLYEKAGEAGGAWSSGVA
                     TLRGNRTGPAEDPMRSAFEAGRRARGKIRRYCTTHRLNRLGTLTYRGEGNHDPYLLRE
                     HLGLFFRALRAGLGGDPFPYVWVPECHKSGHGLHAHFAVGRYIKRSVIEAAWGHGFVH
                     IKLLGDLPVGSGPLDEARRAAGYLSKYIGKGFGDVSTPGLHRYEVAQGFEPQMVKLTG
                     RRLGDVLAQAAEVMGSDPRYLWQSQVAEGWRGPPAVWASWD"
     gene            complement(5402..6793)
                     /locus_tag="VNQ33_00080"
     CDS             complement(5402..6793)
                     /locus_tag="VNQ33_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ96518.1"
                     /translation="MIRAAVYARISSDDGTALGVTRQVEDCRKLAAELGWQVVDEYVD
                     NDVSAYSGKKRPAYERMLSDLSDGLVDAVLAYHADRLTRRPIELEHFVETVSAAGVRH
                     VRFVSGGEMDPGNGDGLLVLRMLGAVAANESASKSRRVRRKLDEVAAAGRPHGGSVRP
                     FGFEDDKITVRPAEAEVVRQLATRYLAGESLRSLCIWLDDEGVRTVKGGLWRSPTLRG
                     LLSSGRIAGLRVHRGEVVGPAVWEPIITPAQRDKILARMADAATTGRRTPRRYLLSGL
                     LRCHRCGGKLYSAARVDTRRYVCMSGPDHGGCGGTLIVAGPVEELVADAVLYRLDTAD
                     LAAALAGRAADDEQAVVLGDQLAADRQQLDELAALYADKAITVREWMAARRPIEDRID
                     QAQRKVARLGNSDALVGLVGNGGELRSRWAGLNLTRQAAIVAAILDHAVIGPGTPGAR
                     AFDANRVELVWRL"
     gene            6816..6959
                     /locus_tag="VNQ33_00085"
     CDS             6816..6959
                     /locus_tag="VNQ33_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96519.1"
                     /translation="MNLALLCPHHHRLIHQAGFTLVRGPTGLVFRRPDGTAIGPPPFA
                     NAA"
     gene            complement(7157..7771)
                     /locus_tag="VNQ33_00090"
     CDS             complement(7157..7771)
                     /locus_tag="VNQ33_00090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ96520.1"
                     /translation="MDAVRRAYGDMAGAYTDRFGTTAQVDPDDLARITRRLAIRPGVV
                     ADAGCGPGHLTAHLQLQGVDAIGIDLVPELLGHARAAHPHGSYVRGSMERLPVPDGSL
                     AGVLAWYSLIHLPPDALDGVLAELRRAVRSGATLVVGFFDGGEHAAFEHKVATAYFWP
                     VDELGRRLEQVGFTEVERWQRPAVPSGPRAHAAIVATADEPTPP"
BASE COUNT         1294 a         2683 c         2682 g         1300 t
ORIGIN      
        1 cgccgggcct atcagcgaag gcagcttcgt gttcacggtt tgggttccgg ttccgccgcg
       61 cttggtcccc gaagaggatc tcgtgctgtt cgagatcgag gacgagcctt tcgagcgtgc
      121 cgcgacgctc agactccacc aggcgatgac gtctctcagc caggcagtgc gacaggtaga
      181 ggatgaccac gctgggatcg aggcctttac cgaccgcctg agtgacggcg tgaccgccgt
      241 cctgtgtgag gctgtcgcga cgatggtcaa cgagtcccgg attggacttg agacgtcgtt
      301 ctcctgggcg ctggaccgac ccgttgctga caggaatcaa ccggtggtct ttgggcgggg
      361 gagccaggaa gtgttgcagg aggctgcccg gcacatcagg agtctcattc ctgaggaaga
      421 gatcgttctc gctggcaatg tcgttcgcct gcatcgcgat gcacgggacg gcaccggcga
      481 ggtgaccatc gctggggtgt tcgctggcga tccagacgag cgcctgaggc gcgtgacgat
      541 gacgttgccg ggcgccgtgt acgacgaggc gattcgagca cacgatgagt tcgacctggt
      601 tgaagtcgtg ggggagctgt atcgacgagg gaaccggctt tacctgcgca accctcgtga
      661 cttcctgcgc atcgagcgct agcaccgtgg agcccgctcg cctccgcgcc gctccgggca
      721 ctgacgtact agggaatgaa tgaccgccga cgcccatagt ctgagtccca tgccggaagg
      781 tgaactggtg cggggtgatc ccgagcaagc cgggtgcggg gagccgtcgt gcacggtgtg
      841 cggtctcgac catgagttcg atctgcctga agggctgatc gaggcctgtc acggtggtga
      901 ggtggtgatc ttcgctggag ctggtatcag caccgaggtc cccgtcgcct tcccaaacac
      961 catctttcag atggctgctc atcgcctcgg catgtcgaga gagaccttgg ggacgttccc
     1021 cgcagcgatg caggagtacg agcgtcgctt cggcagggcc gatctcgttc gtctgatctc
     1081 caagaagttt gactacatcg actcgtaccg cgacctgcag tttcatgcga cgaggttcca
     1141 tagtgatcta gcgacgatgc cctatttccg ggacattgtc acgaccaact gggacaccta
     1201 cttcgaggat tggtgtggtg ccacgccctt tgtcaccggc gaggacatcg cattgtggga
     1261 aatgcctggc cgtagggtgc tgaaaatcca tggctcgatc tcgaaccttg cgagcctggt
     1321 tgccacagac gaggactacg aagagcgcct ggcgaagttg tcgtcagatg tgatgggcgg
     1381 gctacttcga cagttcttgg caaccagaac cgtgatcttt gtcgggtact ctttgacgga
     1441 ctggaacttt cggcgactct acgaggcgct ccgttcagac atgaagggtt tcgcaccgcg
     1501 cgcctacgtg gtttcaccct ttccggtcga ggaaaatgac ctcgatctga cagtcatcca
     1561 tacctctggt gttcacttcc tgaggacaat caaagctcag ttggtaggaa gctgcttcat
     1621 tggcgatgag gcgtacgatc gcgtcaatgt cgttctcacg aaggctgccg aggcagacga
     1681 ggtcgccaaa actgtcccgc acaagaagta cccgtcggtg gtgtattgct ggttctacca
     1741 cgatgggctg agagacgcct gcgaccgaat actgcggctc cgtagaacgg gtgagtattc
     1801 agaccgtcat catgtgtttc acctcgtcaa ctcgtacgac gagtggtgcg acaacgccta
     1861 cgacgaggcc cgataccacg atgctgccta cattgacggg tacatgaacg gcctcatgat
     1921 gctgctcgat gacgactggg gccgggaaga ccgtttgtcc gcctacccag acatggaacc
     1981 gttggcggat tcgattccgt actacttcat gtacgggtca gactcggaga tgcgaaccga
     2041 cgacgatctg gccgaagccc tcctcctgag ccgccgtcgt gcgccgaagg agcggaaggt
     2101 cgctcgggag atcgttggcg ggctcgccga ggacatggtg atctctcaca gccctttcat
     2161 cccggacgcg cccggtctct tcacttgaaa gaccaacgaa actcggaagc gagtgccggg
     2221 ccgctggacc gtcagactcc cgaccaccct tgcgcactag tggtgcccct gacagccgag
     2281 cctctgggcc agcggactat ctgaggcgcg ccgagatcga ccgccaccag ggcctgccgg
     2341 gtgatcgcac ggccgcgacc gctttttcgc cgtatccggc tgtgtagccg cgggcgtagt
     2401 cgtcgccgtg ctgccgggcg agggcatcga cgctgcacgg actgagcccc agcgacgcgt
     2461 cctcgatgcc cgccgtctcg ccgcgctgga actggttcgg tcggtgggcc acggggcagg
     2521 agcctaggcg gatagcccgt tttgggctag acggttgaga ggcgtcctcc tgtgcggatg
     2581 gacaagtcga tctacacccc ggatcaccag cgcctttgcc tgaagctgcg ccagcttcgg
     2641 accgagcgcg gtctcaccca gggcgagctt gccgggctcc tcggcgtcaa ccagacgttc
     2701 gtttcgaagt acgaacgggg cgagcggcgc ctcgatctgc tcgaacttcg ggaggtgtgc
     2761 agggcgctca gcgtctcgct ggtcgagttc gtccgggagt tcgacatctg aggcgcccag
     2821 ggcctcggtg ctgtcggcaa tcctgatttc tttacgggct ggccagtcgg ccggccatgc
     2881 ctggtcaagg gcgggaaatc gggcggtact ctactacaaa atgccctctg acctgcggat
     2941 gtgctggttc agaggctcgt gcctggtggt accttggcca agccataacg gcaacggccg
     3001 gaccccgagg ggcccggccg cagcacagga gccgaagccc ccgcttggtg gaactcgcga
     3061 tggtactcgg ctcctcgaac gaggagaaga gatagatgga ccgttttccg atcgacggag
     3121 cgcagctcca gatccacgct gtggaggtcc gcccggacct gcgtgacggc aaccagaaga
     3181 cccggcctga cggacggctc gtgtgggcgg ttgcgtgcct ggtgcgcttc gacgggcggg
     3241 tcccagagat ggtcacggtg aaggtgccgt ctgcgacgcc gttgaagctc gctgagatgg
     3301 cccgggtcga gttcggtggc ctgtatgccc tgtactggga gaccggtggc cggtcgggga
     3361 tctcggtgca ggccgagtcg gtgagcgccg tggcggcggc gaagtgagcc ccctggtgac
     3421 cgctgccgat cgtgaccgtg tgtcccgagc ggtgatggca ggcggccccg cggttgcggt
     3481 gctgggtgtc gtgggtgggc tgcctcccgt ggtctggctg ctctcggcag cgtgtggcgg
     3541 gttggcggcc tggttccgcc acgacgggcg ccaccaccct tgcctggccg aagacgttga
     3601 ggcggcgctc ggctcgggga ggtcgctgcg ggtcgaggag cggccgctgc cagcgggagt
     3661 ccttctcgtg ctgatcccat cacctccgct caccgacccc cagctccggg ccgtgttggc
     3721 ggggccggtg gcggcgcatg gctacaaggt cgtcagcatc cgccaggcca agcggggcaa
     3781 cctcaccgtg acgctggtgg agctgatgcc atgctgacgc tcgacctcgg ggtctacggc
     3841 cccaacctcg gctccgccgc catcgacttg gccaggacca cgcacgggct ggtgtgcggt
     3901 tccaccgggt cgggcaagag cttcctcgcc cgccgcctca tcgagcaggc ggtcgcgtcg
     3961 gggatggacg tgtcggtggc ggacccgaag gcagccgggg actacaccgg cgccgccgac
     4021 gtggtggccg acgggccgga ggcatcggtg gccctgctcg agcacgtcgc ccgtgccctg
     4081 ccggaccgca tgaccgaacc gtgcgatccg ctgctgatgg tcgtcgacga gctgtcggcg
     4141 gtccagctcc gagtcgtcgg ggagacggcg aaggaggcga aggaccgcgt cgaccgaatc
     4201 catgcggcgc tcggctcgat ctgcctgatg gggcgttccg cacgcatcca cctgctcgcc
     4261 atcacccaac gggccgacac cacagccata ccggggtggg cacgtgatca gtgctcgtgg
     4321 cgggtggcga tgggctggat gtcacccgac gggctccgca tgatgggctt cggtgacgtg
     4381 gccgccccca cggcgtggcc ggggcacggc tgggccgtcg gcatcgtcgg ggcaccgccc
     4441 ggacagccgg tcccgctcca cgtcacccca tgaccgccgg gcgcaccgcc gcccccgctc
     4501 tccccctcga cctcgatccg ccctgcgacc cgagaggatg gaccctcaac ctctacgaga
     4561 aggcaggcga agcgggcggt gcgtggagct ccggcgtcgc cacgctgcgg gggaaccgga
     4621 ccggaccggc agaggatcca atgcgctcgg cgttcgaagc cgggcggcgg gcacggggca
     4681 agatccgccg ctactgcacc acgcaccggc tcaaccggct cggcaccctg acctaccggg
     4741 gcgagggcaa ccacgacccg tacctgctgc gggagcacct gggcctgttc ttccgggcgc
     4801 tgcgcgcggg tctcggcggc gatccgttcc cgtacgtgtg ggtgccggag tgccacaagt
     4861 ccggtcacgg gctccacgcc cacttcgcag tcggccgcta catcaaacgg tcggtgatcg
     4921 aagccgcctg gggccacggc ttcgtccaca tcaagctgct cggtgaccta cccgtcggct
     4981 cggggccgct cgatgaggca cgccgtgccg ccgggtacct gtcgaagtac atcggcaagg
     5041 gcttcggcga cgtgtccacg ccggggctgc accgctacga ggtggcccag gggttcgaac
     5101 cgcagatggt caagctcacc ggccgccgcc tcggtgacgt gctcgcccag gccgccgagg
     5161 tcatgggctc cgatccccgc tacctctggc agtcccaggt ggctgagggg tggcggggac
     5221 cgccagcggt gtgggcgtca tgggactgac cggcgacgag ctggccgcct tcgtcacccg
     5281 ctcgtgcgaa cgccaagggg tgccggtgtt cgtcaccgac gccgccgtcc tggcccgtgt
     5341 cactgcgctg ttgggggcgg gtccgcccgc accgccgaag gcgggaggac ggcccgcacc
     5401 ctcaaagacg ccacaccagc tcgacccggt tcgcatcgaa cgcacgagcg ccaggggtac
     5461 cgggtccgat gacggcgtga tcgaggatcg ccgccacgat cgccgcttgg cgggtgaggt
     5521 tcagtccggc ccaccggctg cgtagctccc cgccgttgcc gacaaggccg accaatgcat
     5581 cggagttgcc gagccgcgcc accttccgct gcgcctggtc gatgcggtcc tcgatcgggc
     5641 gccgtgccgc catccactcc cgaaccgtga tcgccttgtc cgcatacagc gcggcaagct
     5701 catcgagctg ctggcggtca gcagcgagct gatcacccag cacgaccgcc tgctcatcgt
     5761 cagcagccct ccccgccagc gcagcagcca gatcggcggt atcgagccgg tagagcaccg
     5821 catcggccac aagctcctcg accggcccgg cgacgatcag cgtcccaccg cacccgccgt
     5881 gatccgggcc gctcatacag acgtagcggc gggtgtcgac ccgtgccgcc gagtagagct
     5941 tcccgccgca gcggtggcac cgcagcagac ccgacaacag gtagcggcgg ggagtccgcc
     6001 gtccggtcgt cgcagcgtcg gccatgcggg ccaggatctt gtcccgctgg gccggggtga
     6061 tgatcggctc ccacacggcc gggccgacca cctcgccccg gtgcacccgc agccccgcga
     6121 tccgccccga cgacagcaga ccccgcagcg tcggagagcg ccacagaccc cccttcaccg
     6181 tccggacgcc ctcgtcatcg agccagatgc acagcgaccg cagcgactcg ccggcgaggt
     6241 agcgggtggc gagctggcgg accacctcgg cttcggccgg ccggaccgtg atcttgtcgt
     6301 cttcgaaccc gaacggccgg accgacccgc cgtgaggacg acccgccgct gccacctcat
     6361 cgagcttgcg gcgaacccgg cggctcttcg acgctgactc gttcgctgcc accgcaccca
     6421 acatacgcag caccaacaac ccgtcaccgt tgcccggatc catctcaccg cccgacacga
     6481 aacgaacatg gcgcaccccc gccgccgaca ccgtctccac gaagtgctcc aactcgatcg
     6541 gacgacgagt caaccggtca gcgtgatagg cgagcaccgc atcgaccagg ccatcgctca
     6601 ggtccgacag catccgctca taggcaggcc gcttcttgcc cgagtacgcc gacacgtcgt
     6661 tgtccacgta ctcgtccacc acctgccaac ccagctcagc agcgagcttg cggcagtcct
     6721 cgacctgacg ggtcacgccc agggcggtcc cgtcgtccga tgagatgcgg gcgtacacgg
     6781 cggctcggat catgacagca gattagcgat gtgtggtgaa cctcgccctc ctctgccccc
     6841 accaccaccg gctcatccac caggccgggt tcaccctggt ccgaggcccc accggcctcg
     6901 tgttccgaag gcccgacggc accgccatcg gcccacctcc cttcgccaac gccgcttgac
     6961 cacccgacag ccagacgggc cctcgagcca ggtggcggat ggacgaagag aaccacgaac
     7021 gacagatgac gagccacgag caccacgaac accacgaaca ccacgaacac cacgagtacg
     7081 cctgcctcca cgcgggcgta ctcgccgcgc ccggagggcc atcggtcttg gtctcgttgc
     7141 gagccccgcc gtgcggctac ggcggcgttg gctcgtcggc ggtcgccacg atggcggcgt
     7201 gcgccctcgg gccggagggc acggcgggtc gctgccagcg ttcgacctcg gtgaagccga
     7261 cctgctccag ccgacgaccg agctcgtcga cgggccagaa gtaggccgtc gcgaccttgt
     7321 gctcgaacgc ggcgtgctcg ccgccgtcga agaacccgac gacgagcgtc gctcccgagc
     7381 ggacggctcg gcgcagctcg gcgagcacac cgtcgagcgc atcgggcggc aggtggatga
     7441 gcgagtacca ggccaagacg ccggccaacg agccatccgg gaccggaagc cgctccatcg
     7501 agccccgcac gtagctcccg tgcggatgcg cggcgcgggc gtgacccagg agctcgggca
     7561 ccaggtcgat gccgatcgcg tcgaccccct gcaactgcag gtgggcggtg aggtggccgg
     7621 gcccgcagcc ggcatcggcc acgacaccgg gccggatcgc cagccgccgg gtgatgcggg
     7681 cgaggtcgtc ggggtccacc tgcgccgtcg tgccgaaccg atcggtgtac gccccggcca
     7741 tgtcgccgta tgcccgccgg accgcatcca aggcggcgga ccctaccgct gtgtccaggc
     7801 gatggcagga ttcgcgccat ggctgatgcg ggatcgagtc aggtgggaag cggcgacatc
     7861 gggttcgggt cggagcgggc gatccgcgtg ttcgtctcgt cgacgttccg ggacatgcag
     7921 gccgagcgcg aggagctggt gaagcgggtc ttcccgcag
//
LOCUS       DATMFS010000004         3486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_70074, whole genome shotgun sequence.
ACCESSION   DATMFS010000004 DATMFS010000000
VERSION     DATMFS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70074"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VNQ33_00095"
     CDS             <1..765
                     /locus_tag="VNQ33_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ96521.1"
                     /translation="NTRVGELAELVGIDASMLKRYPSELSGGQQQRVGVARALAADPP
                     VLLMDEPFGAVDPIVRAHLQDELLALQARVHKTIVLVTHDIDEAILLGDRVVVLNVGG
                     ILEQVAAPDDLLASPANGFVASFVGNERGIKRLSLARVERLALDAGPVVDRLASGADI
                     KAAADAAGVDWVGATESGRLAGWVPVEDLRLDAAAPLTGTELRPVVAQLRPSSSLRAA
                     LEVILTARSSTALVADEDGTYLGSVTLETIRSGLAS"
     gene            762..1529
                     /locus_tag="VNQ33_00100"
     CDS             762..1529
                     /locus_tag="VNQ33_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96522.1"
                     /translation="MTARRKATYAVVLVALAAFGIWAANRVSGLSEDTQATNPLFRWE
                     YLFTRERTPGELWGKTRQHLELTVVPVLIGIVLSSLLSALILRFGWLRSVVFTFAGVL
                     YTIPSIALFAVLVTYYSNETAAITALTSYTLLILTRNFVAGIDAVPRAALDAADGLGM
                     SRFQRLRKVEIPLALPVILTGIRIATVTVVGLVGISAVIQLGGLAIYIFDGFNRDYTT
                     LMVLGTVATILLAVVLDIVLRGIEWLATPWARRRVAP"
     gene            1526..2311
                     /locus_tag="VNQ33_00105"
     CDS             1526..2311
                     /locus_tag="VNQ33_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ96523.1"
                     /translation="MSLAALLATTEPTNVWQWLNDSSERTVTGDIPTQAWTTLWHSVL
                     AFVIAAAAAIPLAAVLAHFRKAEVLSTWLVNIGRVIPTVTIVAVFVIISLRNGYGFEP
                     WPIVFALVLLALPPLYSNTYTAVRDASPEAVGAARAMGLTELQILRRVELPLATPLIL
                     AASRVAITQVVATEALAALFGGTGLGQYITFGIANRDIYEVQAGAVLVAGLAMSVDLV
                     FWFVTRFLVPKPLRPAPANRRPIHRLRPVQPINRNTPSLGGTP"
     gene            2308..3255
                     /locus_tag="VNQ33_00110"
     CDS             2308..3255
                     /locus_tag="VNQ33_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ96524.1"
                     /translation="MKNRTTTRRWRALSLLAVVGVLALASCSDNKSDAGSDGSTKTST
                     GSGEFADAIRISSADFSEQKVLAQVYGQYLQDQGIAVDIQDPVGTREQIFKALDDDKL
                     DLQLDYSGLAVGFLGGTPTADSQETYDALVPLLKDKGLEASEQSEAQDGDALVALTSW
                     AEENGVTTISDLSGVDGTLTLGGAAECADRPQCLQGYKDTYGLDLKFKAVDYGPPLVA
                     ALKADEVQVAQYQSTAPEISTGQIVELTDDKGLHVAANVVPVFRSAVSTPELIKALNT
                     ISAAITTEDLAAWNQATDVDKEDPTDVATKWLQDNDLLK"
BASE COUNT          508 a         1331 c         1116 g          531 t
ORIGIN      
        1 aacacccgcg tcggcgagct ggccgagctg gtgggcatcg atgcgtcgat gctgaagcgc
       61 tacccctccg agctgtcggg cggccagcag cagcgggtcg gcgtggcccg cgccctcgcc
      121 gccgatccgc cggtgctgct catggacgag cccttcggcg ccgtcgaccc catcgtccga
      181 gcccacctgc aagacgagct gctggcgctg caggcccgcg tccacaagac catcgtcctc
      241 gtcacccacg acatcgacga ggcgatcctc ctcggcgatc gggtggtcgt gctcaacgtg
      301 ggcggcatcc tcgagcaggt ggctgcacct gacgacctgc tggcgtcgcc ggccaacggg
      361 ttcgtggcgt cgttcgtcgg caacgagcgc gggatcaagc gcctctcgct ggcccgggtc
      421 gaacggctcg ccctcgacgc gggccccgtg gtggaccgtc tcgcatcggg ggccgacatc
      481 aaagcggccg ccgacgcggc cggcgtcgac tgggtcggtg ccacggagtc cggccggctc
      541 gccggctggg tccccgtcga ggacctccgg ctcgacgctg ctgctcccct caccggcacg
      601 gagctccgcc cggtggtcgc ccagctgcgg ccgtcgtcga gcctgcgcgc cgcgctcgag
      661 gtgatcctca cggcccgctc gtcgacggcc ctggtggccg acgaggacgg gacctacctc
      721 gggtccgtca ccctggagac gatccggtcg gggctggcgt cgtgaccgcc cgccgcaagg
      781 ccacctacgc cgtcgtgctc gtggccctgg ccgcgttcgg catctgggcg gcgaaccggg
      841 tctccgggtt gagcgaggac acccaggcca ccaacccgct cttccgctgg gagtacctgt
      901 tcacgcgcga gcgcacgccc ggtgagctgt gggggaagac ccgccagcac ctcgagctga
      961 cggtggtccc ggtcctgatc ggcatcgtgt tgtcgtctct gctgtccgcg ctcatcctcc
     1021 gcttcggctg gctgcgaagc gtcgtgttca ccttcgccgg ggtgctctac accatcccga
     1081 gcatcgccct gttcgccgtc ctcgtcacct actactcgaa cgagacggcg gcgatcaccg
     1141 ccctcaccag ctacacgctg ctgatcctga cgagaaactt cgtcgccggg atcgacgccg
     1201 tgccgcgggc ggccctcgac gctgccgacg gactgggcat gagccggttc cagcggctgc
     1261 gcaaggtcga gatcccgctg gcgctgccgg tcatcctcac cgggatccgg atcgcgacgg
     1321 tgacggtcgt cggcctcgtc ggcatctccg cggtgatcca gctcggcggt ctggccatct
     1381 acatcttcga cggcttcaac cgggactaca cgaccctcat ggtcctcggg accgtcgcca
     1441 cgatcctgct ggccgtggtg ctcgacatcg tcctccgcgg gatcgagtgg ctcgccacgc
     1501 cctgggcgcg ccggagggtg gcgccatgag cctcgccgcc ctcctcgcca cgacggagcc
     1561 gacgaacgtc tggcagtggc tgaacgacag ctcggagcgc accgtcaccg gcgacatccc
     1621 gacccaggcg tggaccacgc tctggcactc ggtgctcgcg ttcgtgatcg cggcggcggc
     1681 cgccatcccg cttgccgccg tgctcgccca cttccgcaag gccgaggtgc tgtcgacctg
     1741 gctcgtcaac atcgggcggg tgatcccgac cgtgaccatc gtcgccgtgt tcgtgatcat
     1801 ctcgctgcgc aacggctacg ggttcgaacc ctggcccatc gtcttcgccc tggtcctgct
     1861 cgccctgccg ccgctgtact ccaacaccta cacggcggtg cgcgatgcgt cacctgaagc
     1921 cgtgggcgca gcccgggcca tgggcctcac ggagctgcag atcctgcgcc gggtcgagct
     1981 gcccctcgcc acgcccctga tcctcgccgc gagccgcgtc gccatcaccc aggtggtcgc
     2041 caccgaggcc ctcgccgccc tgttcggcgg caccgggctc gggcagtaca tcaccttcgg
     2101 gatcgcgaac cgcgacatct acgaggtcca ggccggtgcc gtcctcgtcg cagggctcgc
     2161 catgtcggtc gacctcgtct tctggttcgt gacccgcttc ctggtcccga agccgctgcg
     2221 ccccgccccg gcgaaccgac gccccatcca ccgtctccgc cctgtccaac ccatcaaccg
     2281 caacacacca tcccttggag ggaccccatg aagaaccgca ccacaacccg ccgctggcgg
     2341 gccctgagcc tcctcgccgt ggtcggcgtg ctggccctgg cctcgtgcag cgacaacaag
     2401 tcggatgccg ggtccgacgg cagcaccaag acgtccaccg gctcgggcga gttcgctgat
     2461 gccattcgca tcagcagcgc cgacttcagc gagcagaagg tcctcgccca ggtctacggc
     2521 cagtacctcc aggaccaggg catcgccgtc gacatccaag atcccgtggg gacgcgcgag
     2581 cagatcttca aggccctcga cgacgacaag ctcgacctcc agctcgacta ctccggcctg
     2641 gccgtcggct tcttgggcgg gacgcccacc gccgactcgc aggagaccta cgacgcgctc
     2701 gttcccctcc tgaaggacaa gggcctcgag gcgagcgagc agtccgaggc ccaagacggc
     2761 gacgccctcg tggccctcac cagctgggcc gaggagaacg gggtcacgac catctccgac
     2821 ctgtcgggcg tcgacggcac cctgaccctg ggtggcgccg ccgagtgcgc cgaccggccg
     2881 cagtgcctcc agggctacaa ggacacctac gggctcgacc tcaagttcaa ggcggtcgac
     2941 tacggcccac cgctcgtcgc cgcgctcaag gccgacgagg tccaagtcgc ccagtaccag
     3001 tcgacggcac cggagatctc caccggtcag atcgtcgagc tcacagacga caagggcctg
     3061 cacgtcgccg ccaacgtcgt cccggtgttc cgctccgcgg tgagcacgcc cgagctgatc
     3121 aaggccctga acaccatcag cgccgccatc accaccgagg acctcgccgc ctggaaccag
     3181 gcgaccgatg tcgacaagga ggacccgacc gacgtcgcca ccaagtggct ccaggacaac
     3241 gacctcctga agtaggtcac cgccgcagtt ccgatgacgg ccgtcgtcgc cccctggggc
     3301 ggcggcggcc gcttcgcgtc tcggcccggc tcaggtgatg cgggtgaaga acatctgctg
     3361 ggccgcccag gccacgagct tcgggccacc gtcagcccgc ggatcccaca gggccatctc
     3421 agccgaggcg cagccgtcac cggcgaggtg ggccttgctg tgggcgagca tccaccccgg
     3481 cgtggc
//
LOCUS       DATMFS010000005         3770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_186170, whole genome shotgun sequence.
ACCESSION   DATMFS010000005 DATMFS010000000
VERSION     DATMFS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186170"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3770
                     /locus_tag="VNQ33_00115"
     CDS             <1..>3770
                     /locus_tag="VNQ33_00115"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HWJ96525.1"
                     /translation="VLAVLAAAVPVGAAIARSAPAGASGATLVVTSLGDAPDADLGDG
                     MCDTGDGTCTLRAALDEAGASTGEVIGFSVSGTIAVESTLRIRSPLTIDGTGRRVTVS
                     GGGSVGVFATAIEVAGGGSATIRALTIADGFDGSTGGAAIFNSAASGGEGLVLDGVTV
                     RDNTAAAGAVFGGAVVNTSRLTIRNSTFSGNDADAGTDGSDVSNVFSGSASITQSTFT
                     GSAGSSAVSSTSGDPATITSSIVSGPGFACSGTFTGSGNVTTDDDAICPGTAGVTPSG
                     LGLAALADNGGPTNTVRLLPGSAAVDAGSAGGCTATDQRGAGRPKGAGCDAGAYELDP
                     ATATSLTAAPASVPFGGSVTVETTVAASGEPVVPTGEVELFDGTTSLGKVPLVDGSAS
                     TDLTALAAGTHDLEAVYVPDAGLASSRSAVRTVTVQRAVPTVTLTSSGSPTGGGEAVS
                     FQVAVTGGGSVPTGSVTVKDAATAIETVALDAAGRAEVVTSSLSGGPHVIVADYSGDA
                     NHAPASSAALAHEVVGGSTTVLRGPAGPTVFGNDAVFEVDVEPVPGGRTPTGLVTLFT
                     GPTPVGFANLDATGSATIPVTVLPPGTSSVQASYAGDGYNGASTSSLVDHVVTSAATK
                     TTLSVSPSSTVFGGAVTVDVAVTAVGSPAVPSGTVAIANGSTVITRLTLEADGTASAT
                     LPTQPAGTYALHAEFDGATGLDPSTSTVTGHEIAKASTGVALTASSPTSVAGEQVTLT
                     AAVASTSSAEVPVGAVTFRDGTTELGTVAVDGSGAARLDTRAIAVGSRNLTATFDGSN
                     DFASAPSSPLGHTVAKAGVQVSIVASPARTWFGTAVTIDVVVAAASPGSGLPSGSVTI
                     RDGSAVLGTPALDGSGRAALTVSTIAVGERTLTAGYAGDGSFETATASTTLTVDADTT
                     TVIVQTSAPEQGYGRPVTLTAIVQSAGGLVPTGTVTFTGGSRTLGTAMLDGSGRASVT
                     VADLPVGSTLVVAAYAGDGRFAPGSGQRLQQVTASPTSTSLAVVPTSPTVADVVTLTV
                     TVAAPGATLEPAGSVEFLDNGAVIGTVALDGGGQAAFSRRFDRSSHELQARFVATTAW
                     QASSSGRETITPSRVGASIAMSATPTSVLIGGSVTLTAAVPTVPGVPAPTGVINVTDA
                     SGALGSVPLVDGVATLEATAPVTPGPWIVSAWYSGDLNYEPVAPTATFVEVAASPTIL
                     AVVTTPNPGFALSGDVTVRAHVAAGAGSPPVAGKVRFASDSPVLTSETATVDA"
BASE COUNT          464 a         1433 c         1392 g          481 t
ORIGIN      
        1 gtcctcgccg tgctggccgc ggccgtaccc gtgggggcgg ccatcgcccg ctccgccccg
       61 gccggagcct ccggtgccac cctcgtcgtg accagcctgg gcgacgcccc cgacgcggac
      121 ctcggtgacg ggatgtgcga caccggtgac gggacgtgca cgctgcgggc ggcgctcgac
      181 gaggctggcg cctccacggg cgaggtcatc ggcttctcgg tcagcggcac gatcgccgtc
      241 gagtcgacgc tgcggatccg gagcccgctc accatcgacg ggaccggccg gcgggtgacg
      301 gtgagcggcg gcggttcggt cggggtcttc gccaccgcca tcgaggtcgc gggcggaggg
      361 tcggccacga tccgggcgct caccatcgcc gacgggttcg acggctccac cggcggcgcg
      421 gccatcttca actcggcggc ctccgggggt gaggggctcg tcctcgacgg cgtgaccgtg
      481 cgggacaaca cggccgcggc gggcgccgtg ttcggcggag ccgtcgtcaa cacctcccgg
      541 ctgaccatcc gcaacagcac cttctccggg aacgacgccg acgctggcac cgacggcagc
      601 gacgtgtcca acgtgttctc cggaagcgcg tcgatcacgc agtcgacgtt caccggctcg
      661 gcgggctcgt cggccgtctc gtccaccagc ggcgacccgg ccaccatcac gagctcgatc
      721 gtgagcggcc ctggcttcgc ctgctcgggc acgttcaccg gttcgggaaa cgtcaccacc
      781 gacgacgatg cgatctgccc gggcaccgcg ggtgtcaccc cgtccgggct cgggctggcg
      841 gccctggccg acaacggcgg gccgacgaac acggtccggc tgctgcccgg gagcgcggcc
      901 gtcgatgccg ggagcgctgg tgggtgcacg gcgaccgacc agcggggcgc gggccggccg
      961 aagggtgcgg gctgcgacgc cggtgcctac gagctggacc cggccaccgc cacctcgctc
     1021 acggccgctc cggcttcggt cccgttcggc ggcagcgtga cggtcgagac gacggtggcg
     1081 gcgtcgggcg agccggtggt ccccaccggc gaggtcgagc tgttcgacgg caccacctcg
     1141 ctcgggaagg tgccgcttgt cgacggctcc gcgtcgacgg acctcacggc gctcgccgcc
     1201 ggcacccacg acctcgaggc ggtctacgtc ccggacgcgg gtctcgcctc cagcaggtcg
     1261 gcggtcagga ccgtcaccgt gcagcgggcg gtgccgacgg tgaccctgac ctcgtcgggc
     1321 agcccgacgg gcggtggcga ggcggtgtcg ttccaggtgg cggtgacggg cggcggctcg
     1381 gtgcccaccg ggtcggtgac cgtgaaggac gcggcgacgg cgatcgagac cgtggcgctc
     1441 gacgctgccg gccgggccga ggtcgtcacc tcgtcgctga gcggcgggcc gcacgtgatc
     1501 gtggccgact actcgggcga cgcgaaccac gcgcccgcca gttcggcggc cctggcgcac
     1561 gaggtggtcg ggggcagcac caccgtgctc cgcgggccgg ccggtccgac ggtgttcggc
     1621 aacgacgccg tgttcgaggt ggacgtggag ccggtgccgg gcgggcgcac cccgaccggc
     1681 ctggtcacgc tgttcaccgg gccgacgccg gtcgggttcg ccaacctcga tgcgaccggc
     1741 tcggccacga tcccggtcac ggtcctgccg cccgggacga gctcggtgca ggcctcctac
     1801 gcgggcgacg ggtacaacgg cgcgtcgacg tcgagcctgg tcgaccacgt cgtgacctcg
     1861 gcggcgacca agacgaccct gtccgtgtcg ccgtcgtcga ccgtgttcgg cggcgccgtc
     1921 accgtcgatg tcgcggtcac ggcggtgggc agcccggcgg tgccgtcggg gacggtcgcc
     1981 atcgccaacg ggtccacggt gatcacccgg ctgacgctcg aggccgacgg caccgcgtcc
     2041 gcgacgctgc ccacccaacc cgccgggacc tacgccctgc acgccgagtt cgacggggcg
     2101 accggtctgg acccgtcgac gtcgacggtc accggccacg agatcgccaa ggccagcacc
     2161 ggcgtcgccc tcacggccag ctcgccgacg tcggtggcgg gcgagcaggt cacgctcacc
     2221 gccgccgtgg cgagcacgag ctcggccgag gtgcccgtcg gcgccgtgac cttccgcgac
     2281 ggcaccacgg agctcggcac ggtcgccgtc gacggatccg gcgcggcccg gctcgacacc
     2341 cgggccatcg ccgtcggctc gcggaacctg acggccacgt tcgacggcag caacgacttc
     2401 gcctcggccc catcgagtcc actcggccac accgtggcca aggccggcgt gcaggtgagc
     2461 atcgtggcgt cgccggcccg gacctggttc ggcaccgctg tgaccatcga cgtggtggtg
     2521 gcggccgcat ctcccggctc gggcctgccg agtggatcgg tcacgatccg cgacgggtcg
     2581 gccgtgctcg gcaccccggc gctcgacggg agcgggcgag cggcgctcac ggtcagcacg
     2641 atcgccgtgg gcgagcggac cctgaccgcc ggctacgccg gcgacggctc gttcgagacc
     2701 gccacggcga gcacgaccct caccgtcgat gccgacacga cgaccgtcat cgtgcagacg
     2761 tcggcgcccg agcaggggta cgggcggccg gtgacgctga ccgccatcgt ccagagcgcg
     2821 ggcggcctcg tccccaccgg gaccgtcacc ttcaccggag ggagccggac cctcggcacg
     2881 gcgatgctcg acgggtcggg ccgggccagc gtgaccgtgg ccgacctgcc cgtcgggtcg
     2941 acgctcgtcg tggcggccta cgccggcgac ggccgcttcg cccccggatc cggccagcgg
     3001 ctccagcagg tcacggcctc gccgacgtcg accagcctgg cggtggtccc gacttcgccc
     3061 acggtcgccg acgtggtcac cctcacggtg acggtcgccg cccccggcgc gaccctggag
     3121 cccgccgggt ccgtcgagtt cctcgacaac ggcgccgtca tcggcacggt cgccctcgac
     3181 ggcggcggcc aggcggcgtt ctcccggcgg ttcgaccgca gctcgcacga gctccaggcc
     3241 cggttcgtcg ccacgacggc gtggcaggcc agctcctcgg gccgcgagac catcaccccg
     3301 agccgggtcg gcgccagcat cgccatgagc gccacgccga catccgtgct catcggcggc
     3361 agcgtcacgc tgaccgcggc ggtgcccacg gtccccggtg tcccggcgcc gacgggcgtc
     3421 atcaacgtca ccgatgcatc gggcgccctc gggtcggtgc cgctcgtcga cggggtggcc
     3481 acgctcgagg cgaccgcgcc ggtgacgccg ggcccgtgga tcgtcagcgc ctggtactcg
     3541 ggtgacctga actacgaacc ggtggcgccg acggccacgt tcgtcgaggt ggcggcgtcg
     3601 cccaccatcc tggcggtcgt caccacgccg aacccgggct tcgccctcag cggcgacgtg
     3661 accgtccggg cccacgtcgc cgctggtgcc gggagcccgc ccgtcgccgg caaggtgcgc
     3721 ttcgcctcgg acagcccggt gctcacgtcc gagacggcga cggtggacgc
//
LOCUS       DATMFS010000006         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_23525, whole genome shotgun sequence.
ACCESSION   DATMFS010000006 DATMFS010000000
VERSION     DATMFS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23525"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VNQ33_00120"
     CDS             <1..1084
                     /locus_tag="VNQ33_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ96526.1"
                     /translation="HRPVQVADEDGVRVVRVWLWASMGSGIMRMVNYASFGAMSLLGL
                     ARTRPARWTVVEYPTLFGALPAAAWCRLRRRQVVVNVADLWVDAIVEFGALREGLVVR
                     ILRRLERWMLTHATAVTAVTDGVRDALIDKGVSPDRICWLPNGADARMFSPGPADDDV
                     DAALGLDPGDHLFLYAGTQGYVHGLEPMLDAAEALRDEPIRFVFVGGGSERPELEAGA
                     AARGLDKVAFLDPVPPEEIGRYLRRATAGLASIRELELFKSVRSAKMFPVMATGKPVL
                     YAGADEGAALIAETGAGLVVPHGDVEALVAAVRRLVGAPDEAAAMGAAGRRWIDDEGS
                     WTVLIERWLTDLARVEATAGAPSPSA"
     gene            1124..>2107
                     /locus_tag="VNQ33_00125"
     CDS             1124..>2107
                     /locus_tag="VNQ33_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWJ96527.1"
                     /translation="MAEIEIGMGKSGRRAYGLDDVALVPSRRTRDPEDIDLSWEIDAF
                     RFELPIMASAMDSVVSPASAVAIGELGGVGVLALEGLWTRYDDPDAVLAEIAGLADPG
                     LPGGVATARLQELYAEPIKPELIRDRIKEIRGAGVTSCASITPQRLPAFADALLAAEL
                     DLLVVQGSVVSAEHVTKDGDPLNLKTFVRRFDIPVIVGGCASYQAASHLMRTGAAGIL
                     VGVGSGATSTTRRVLGVGAAQATAIADARAARMRHLDETGVYVHLIADGGIRTGGDIA
                     KAVACGADAVMVGAPLASASDAPGRGWHWGLNGGHADLPRAERVPAPPVGTL"
BASE COUNT          251 a          789 c          768 g          299 t
ORIGIN      
        1 gcaccgcccg gtgcaggtgg ccgacgagga cggcgtccgc gtcgtgcgcg tgtggctgtg
       61 ggcctcgatg ggcagcggga tcatgcgcat ggtcaactac gcgtcgttcg gagccatgtc
      121 gctgctcggc ctggcccgga cgcggccagc ccgctggacc gtcgtcgagt acccgaccct
      181 gttcggcgcg ctgccggcgg ccgcctggtg ccggctccgg cgccgccagg tggtcgtcaa
      241 cgtggccgat ctgtgggtgg acgccatcgt cgagttcggc gcgctgcgcg aggggctggt
      301 ggtgcggatc ctccgccgcc tcgagcgctg gatgctcacc cacgccaccg cggtcacggc
      361 cgtcaccgac ggcgtgcgcg atgcgctcat cgacaagggc gtgtcccccg atcggatctg
      421 ctggctgccc aacggggccg acgcccgcat gttctcgccc ggccccgcgg acgacgacgt
      481 cgacgccgcc ctcggcctcg atcccggcga ccacctcttc ctctacgccg ggacccaggg
      541 ctacgtgcac ggcctggagc cgatgctcga cgccgccgag gccctgcgcg acgagcccat
      601 ccgcttcgtc ttcgtcggcg gtgggtccga gcgcccggag ctggaggccg gcgccgcggc
      661 ccgggggctc gacaaggtcg ccttcttgga cccggtgccg cccgaggaga tcggacggta
      721 cctccgccgg gccacggcgg gactggcctc gatccgagag ctcgagctgt tcaagtccgt
      781 gcgctcggcc aagatgttcc cggtcatggc gacgggcaag ccggtgctct acgcgggtgc
      841 ggacgagggc gcggcgctga tcgccgagac cggtgccggg ctcgtcgtcc cgcacggcga
      901 cgtcgaggcg ctggtcgccg ccgtgcgccg tctcgtggga gcgcccgacg aggcggccgc
      961 gatgggcgcg gccggacgcc gctggatcga cgacgagggc agctggacgg tgctcatcga
     1021 gcgctggctg accgatctgg cgcgcgtcga ggccacggcc ggggcccctt cgccgagtgc
     1081 gtagactcgt gcctttccct ggccagggtt gaggagaccg tccatggctg aaatcgagat
     1141 cggcatgggc aagtcgggcc gtcgggccta cgggctcgac gacgtcgcgc tcgtcccgtc
     1201 gcgccgcacc cgtgaccccg aagacatcga cctgtcctgg gagatcgacg ccttccgctt
     1261 cgagctcccg atcatggcct cggccatgga cagcgtggtg agcccggcca gcgccgtggc
     1321 catcggcgag ctcggcggcg tcggcgtctt ggcgctcgag ggcctgtgga cgcgctacga
     1381 cgacccggac gccgtcctgg ccgagatcgc cgggctggcc gacccgggcc tgcccggcgg
     1441 cgtcgccacc gcccgcctcc aggagctgta cgccgagccg atcaaaccgg agctgatccg
     1501 cgaccgcatc aaggagatcc ggggcgcagg cgtcacgtcg tgcgcctcga tcacgccgca
     1561 gcggctgccc gcgttcgccg acgccctgct ggccgccgag ctcgacctgc tcgtcgtgca
     1621 ggggtcggtg gtgtcggccg agcacgtcac caaggacggc gatccgctca acctgaagac
     1681 gttcgtccgg cgcttcgaca tccccgtgat cgtcggcggc tgcgccagct accaggcggc
     1741 cagccacctc atgcgcaccg gcgccgccgg catcctcgtg ggcgtcgggt ccggggccac
     1801 ctccaccacc cgccgggtcc tcggcgtggg tgctgcgcag gccacggcga tcgcggatgc
     1861 ccgcgccgcc cgcatgcgcc acctcgacga gaccggtgtg tacgtccacc tcatcgccga
     1921 cggcggcatc cgcaccggcg gcgacatcgc caaggccgtc gcctgcggcg ccgacgccgt
     1981 catggtgggc gccccgctgg cttctgcttc cgacgccccc gggcgcggct ggcactgggg
     2041 gctcaacggg ggccacgccg acctgccccg tgcggagcgg gtcccggcac cgccggtcgg
     2101 cacgctg
//
LOCUS       DATMFS010000007         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_279338, whole genome shotgun sequence.
ACCESSION   DATMFS010000007 DATMFS010000000
VERSION     DATMFS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279338"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1668)
                     /locus_tag="VNQ33_00130"
     CDS             complement(46..1668)
                     /locus_tag="VNQ33_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWJ96528.1"
                     /translation="MNQRQTRILAIALAVIVVLGLLGAIAAPLLAQPTRPVAPTAPAG
                     TSIEPWSQVSGTDGRQYIADQQGRALQFRGMNVKTEHPAADASDQLLADAAKRGFNLL
                     RLSVYWDEMEPSDDAWNEAYFAEVQTVLERAEAHGIWVVLTMHQDNFNTKFGGKGMPD
                     WVTDDEGLPFEPQDVWFLNALQPALMEAWENLYERPAFRAAQIDAWQEIVTRFHQDPA
                     VIGYDLLNEPFGKIRDGENLVTAIERFETTQLTPMYQRLTTAIRAIEPKQWVFVEAPN
                     QASLGIRTWLGAIDGGNIAFFPHLYDSSIESATYTPGGEVKYDPKFFDTYGGVIDVYP
                     DAHHVPVLFGEWGVAHPEAKGMDQFVADAIGLMEDHGSGWTMFNGCRGTGYCPFDAEG
                     NDRPGIGQIVQPYAQAIAGAPTSFHWDADAKTLIVVFTDGAATGTTDLVVPTGTVYPD
                     GWVVETSDADGTWSSTAAVGLDPNVEVVSVSVAATSGPHAICLKPAGAAAGCKVPVAE
                     VPVSTSTTTTAAPVTDDAAGAVPVAGQAAYTG"
     gene            complement(1683..>2636)
                     /locus_tag="VNQ33_00135"
     CDS             complement(1683..>2636)
                     /locus_tag="VNQ33_00135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HWJ96529.1"
                     /translation="SKDHFDSGGDPATTNNKMELMAVLRAIESNPGPLTVVMDSTYVK
                     DGLEKWSVNWIKNGWRTKAKEPVKNREIWEPLIAARDARRHELSFEWVKGHSGDRMND
                     LVDELAVVQRDLFRASIGGTVAGAPGGGGKLSDLPPEEQRAERRARDGRIPSGRLLLV
                     MGLTPDGLGGWDPNPTTDKVRDELRQIIEAQAAYTPDLKVLTGLRPGAELLGAEAAIL
                     AGVPYAAVLGFPDQDRNLKPASKARFADLVGRADDVVQLEKKSPKDRDEFIKAMRRRD
                     VWLSTAADEALLVWDEADDRLDRLHSDLDARLGDRLVVLHP"
BASE COUNT          351 a          914 c          938 g          433 t
ORIGIN      
        1 gacgtcggtg aacgtgccgc cggtgtcgac gccgatccgc acggctcagc cggtgtaggc
       61 ggcctggccg gccaccggca cggcgcccgc cgcgtcgtcg gtgaccggcg cggcggtcgt
      121 ggtggtggag gtcgagaccg ggacctcggc caccggcacc ttgcacccgg cggcagcacc
      181 agccggcttc aggcagatgg cgtgcgggcc gctggtggcc gccacgctga ccgacaccac
      241 ctcgacgttc gggtccagcc cgacggccgc cgtggacgac cacgtgccgt cggcgtcgct
      301 ggtctcgacc acccacccgt cggggtacac ggtgccggtc gggaccacga ggtccgtggt
      361 ccccgtggcg gcgccgtcgg tgaacacgac gatcagggtc ttggcgtcgg cgtcccagtg
      421 gaacgacgtc ggcgcgccgg cgatggcctg ggcgtagggc tggacgatct ggccgatgcc
      481 cggacggtcg ttgccctcgg cgtcgaacgg gcagtacccg gttccccggc acccgttgaa
      541 catcgtccaa cccgaaccgt ggtcctccat caggccgatg gcgtcggcca cgaactggtc
      601 catgcccttc gcctcggggt gggccacgcc ccactcaccg aacagcacgg gcacgtggtg
      661 ggcatccggg tagacgtcga tgaccccgcc atacgtgtcg aagaacttcg ggtcgtactt
      721 cacctcgcca ccgggcgtgt aggtggcgct ctcgatgctg gagtcgtaca ggtgcgggaa
      781 gaacgcgatg ttgccgccgt cgatcgctcc gagccaggtt cggatgccga gcgacgcctg
      841 gttgggggcc tccacgaaca cccactgctt cggctcgatg gcccggatcg ccgtggtcag
      901 gcgctggtac atgggcgtga gctgggtggt ctcgaaccgc tcgatggccg tgacgaggtt
      961 ctcgccgtcg cggatcttgc cgaacggctc gttgaggagg tcgtagccga tgaccgccgg
     1021 atcctggtgg aagcgggtga cgatctcctg ccacgcatcg atctgggcgg cgcggaacgc
     1081 cggccgctcg tacaggttct cccaagcctc catgagcgcc ggctgcaggg cgttcaggaa
     1141 ccacacgtcc tgcggctcga agggcagtcc ctcgtcgtcg gtcacccagt cgggcatccc
     1201 cttgccgccg aacttggtgt tgaagttgtc ctggtgcatc gtcaacacga cccagatgcc
     1261 gtgggcctcg gcccgctcga ggaccgtctg gacctcggcg aagtaggcct cgttccaggc
     1321 gtcgtcgctg ggctccatct cgtcccagta gaccgagagg cgcaggaggt tgaagccccg
     1381 cttcgccgca tcggccagca gctggtcgct ggcgtcggcg gccgggtgct cggtcttcac
     1441 gttcatgccc cggaactgca gggcccggcc ttgctggtcg gcgatgtact ggcgtccgtc
     1501 ggtcccgctg acctgggacc agggctcgat gctggtgccg gccggcgccg tcggggccac
     1561 cgggcgggtc ggctgggcga ggagcggcgc ggcgatggcg ccgagcagcc ccagcacgac
     1621 gatgacggcg agggcgatcg cgaggatccg ggtctggcgc tggttcacgg gtccgacgct
     1681 agtcaggggt gcaggaccac gagccggtcg cccaggcgcg catcgagatc cgagtgcagg
     1741 cggtcgagcc gatcgtcggc ctcgtcccac accagcaggg cctcgtccgc agccgtcgac
     1801 agccacacgt cgcgccggcg catggccttg atgaactcgt ccctgtcctt cgggctcttc
     1861 ttctcgagct ggaccacgtc atcagcccgg ccgaccaggt cggcgaagcg ggccttcgac
     1921 gccggcttga ggttgcggtc ctgatccggg aagccgagca cggcggcgta cgggacgccg
     1981 gcgaggatcg ccgcctcggc acccagcagc tcggcgccgg gtcggaggcc ggtgagcacc
     2041 ttgaggtccg gcgtgtacgc cgcctgggcc tcgatgatct ggcgcagctc gtcgcgcacc
     2101 ttgtcggtgg tggggttcgg gtcccagccg ccgaggccgt cgggcgtgag gcccatcacc
     2161 agcaggaggc ggccgctcgg gatgcgcccg tcacgggccc ggcgctcggc ccgctgctcc
     2221 tcgggcggca ggtcgctgag cttcccgccg ccaccaggcg cgccggcgac ggtcccgccg
     2281 atcgacgctc ggaacaggtc gcgctgcacg acggccagct cgtcgacgag gtcgttcatg
     2341 cgatcacccg agtggccctt gacccactcg aacgacagct cgtggcgtcg ggcgtcgcgg
     2401 gccgcgatca gcggctccca gatctcccgg ttcttgaccg gctccttggc cttggtcctc
     2461 cagccgttct tgatccagtt gaccgaccac ttctccaatc cgtccttcac gtaggtggag
     2521 tccatgacga cggtgagcgg gccggggttc gactcgatgg cccgcagcac ggccatcagc
     2581 tccatcttgt tgttggtggt cgccgggtct cccccgctgt cgaagtggtc cttcga
//
LOCUS       DATMFS010000008         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_710, whole genome shotgun sequence.
ACCESSION   DATMFS010000008 DATMFS010000000
VERSION     DATMFS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNQ33_00140"
     CDS             complement(<1..221)
                     /locus_tag="VNQ33_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWJ96530.1"
                     /translation="MDLYPAIDLRGGQCVRLYQGDFAQETVYGDDPVAQAKVFADAGA
                     PWIHVVDLDAARTGEAVNRPVIAAIVQAVA"
     gene            complement(475..1116)
                     /gene="hisH"
                     /locus_tag="VNQ33_00145"
     CDS             complement(475..1116)
                     /gene="hisH"
                     /locus_tag="VNQ33_00145"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWJ96531.1"
                     /translation="MTRPLIAVLDYGIGNLRSAQKALEKVGGDARLTDDHGLIADAAG
                     IVLPGVGNFGRCLEALDAAGLRDVAAAAAQEAAGGSGRPFLGVCVGMQMLFDGSDESP
                     DVPGLGILPGRVRLLPDSVKRPQMQWNVLEWASPSGSATHLADGQPDPCWMYFVHSYW
                     ADAAPGDIAATCEYGVAAVAAVQRGRLWATQFHPEKSAANGLHLLERFVTACA"
     gene            complement(1127..1720)
                     /gene="hisB"
                     /locus_tag="VNQ33_00150"
     CDS             complement(1127..1720)
                     /gene="hisB"
                     /locus_tag="VNQ33_00150"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWJ96532.1"
                     /translation="MTRSASKQRTTKETSISIALDVDGPTGAVSASTGLPFFDHMLDQ
                     IGRHGGFDLSIEATGDLEVDGHHTVEDVGILLGEVFKEALGDKAGVRRFASGLFPLDE
                     ALIEVALDLSGRPYFVYDVPFGEVLPLGSPPFNPEMAEHFWQSFATSAGITLHVSKKA
                     GVNTHHVVEATFKGVARCLRDAVRVEGTAVPSTKGSL"
     gene            complement(1717..>2648)
                     /locus_tag="VNQ33_00155"
     CDS             complement(1717..>2648)
                     /locus_tag="VNQ33_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ96533.1"
                     /translation="VAATEWHRYPDRQATELRRRLAAVHGVGSGQVFVANGSNEVLQT
                     LSLTYGGPGRSIAVFEPTYAMHSQIARVAGSTVAEGARGADLALDLDEVRRVVAEARP
                     AITYLCSPNNPTGMVESAETVTEVLDLVEGIGGLLVVDEAYGQFAPWSALELLSEDRS
                     LVVTRTYSKTWSMAAARLGYLLGPSWVVAELEKVVLPYHLDALKQAAGTIALDFVDDM
                     ERRVSILTEERGRVDAALRELPLEVWPSAANFILFRPTTADANDVWQQLVDRSVLVRN
                     CSGWPGLTGCLRVTIGTTDEDDRFLTALREVLS"
BASE COUNT          393 a          920 c          979 g          356 t
ORIGIN      
        1 gccacggcct ggacgatggc ggcgatgacg ggccggttga cggcctcgcc ggtgcgggcg
       61 gcgtcgaggt cgaccacgtg gatccacggc gccccggcgt cggcgaacac cttggcctgg
      121 gccacggggt cgtcgccgta cacggtctcc tgggcgaagt cgccctggta gaggcgcacg
      181 cactgcccgc cacgaagatc gatcgccgga tacaggtcca tcaccccatc atcgccgccc
      241 cgcctcgatc ggccccaacg cacccgatgt cctcgtctgg tccggtggcg ttcgtcggac
      301 cgggccgtgg gtgcttggcc ttgttccact tcgcagcgac gatccggtca tcaccgacgt
      361 ctgggagtcc cgaccgagcc cctttcgggt cgcctgagca ccgccacggt gcaccgacga
      421 cccgaaatcg ggtggggtgg tggcgggtcc gggccgactc gacggcgggc ggtgtcaggc
      481 gcaggcggtg acgaagcgct cgaggaggtg gaggccgttg gcggccgact tctcggggtg
      541 gaactgggtg gcccacagcc ggccgcgctg gaccgcggcg acggcggcga cgccgtactc
      601 gcaggtggcg gcgatgtcgc caggagcagc gtcggcccag tacgagtgca cgaagtacat
      661 ccagcacggg tcgggctggc cgtcggccag gtgcgtggcg gaaccgctgg gcgacgccca
      721 ctcgagcacg ttccactgca tctgcggccg cttgaccgag tcgggcagga gccggacccg
      781 tcctggcagg atgccgagcc cgggcacgtc gggcgactcg tcggacccgt cgaacagcat
      841 ctgcatcccg acgcacacac cgaggaacgg ccggcccgaa ccgcccgccg cctcctgcgc
      901 cgccgcggcc gcgacatccc gcagcccggc cgcgtcgagc gcctcgaggc agcgaccgaa
      961 gttgccgaca ccgggcagca cgatgccggc ggcatcggcg atcaggccgt ggtcgtcggt
     1021 caggcgggcg tcgccgccca ccttctcgag cgccttctgc gccgagcgca ggttgccgat
     1081 gccgtagtcg agcacggcga tgagcggacg cgtcaccgcg ggcccctcac agcgagccct
     1141 tggtggacgg cacggcggtg ccctccaccc gcacggcgtc gcgcaggcag cgggccacac
     1201 ccttgaaggt ggcctcgacc acgtggtggg tgttgacccc ggccttcttg ctcacgtgca
     1261 gggtgatgcc cgccgaggtg gcgaaggact gccagaagtg ctcggccatc tccgggttga
     1321 acggcgggct gccgagcggc aggacctcgc cgaagggcac gtcgtagacg aagtagggcc
     1381 ggcccgacag gtcgagcgcc acctcgatga gcgcctcgtc cagggggaac agccccgagg
     1441 cgaaccggcg cacgccggcc ttgtcgccca gcgcctcctt gaacacctcg ccgagcagga
     1501 tgccgacgtc ttcgaccgtg tggtggccgt cgacctcgag gtcacccgtc gcctcgatgc
     1561 tcaggtcgaa cccgccgtgg cggccgatct ggtcgagcat gtggtcgaag aacgggaggc
     1621 cggtcgacgc cgacacggca ccggtggggc cgtcgacgtc gagcgcgatg gagatcgagg
     1681 tctccttggt ggtccgctgc ttggaggcgg aacgggtcat gacaggacct ctcggagggc
     1741 ggtgaggaag cggtcgtcct cgtcggtggt gccgatggtg acgcgcaggc agccggtgag
     1801 gccaggccag cccgagcagt tgcgcacgag cacggaccgg tcgacgagct gctgccagac
     1861 gtcgttcgca tcggccgtgg tggggcggaa caagatgaag ttcgcggccg agggccacac
     1921 ctcgagcggc agctcgcgca gggcggcatc gacccggccc cgctcctcgg tgaggatcga
     1981 gacccggcgc tccatgtcgt cgacgaagtc caaggcgatg gtgccggccg cctgcttgag
     2041 cgcgtcgagg tggtacggca gcaccacctt ctccagctcg gctacgaccc acgacgggcc
     2101 gagcaggtac cccaaccggg cggcggccat cgaccacgtc ttggagtagg tgcgggtgac
     2161 gacgagcgag cggtcctcgg acaagagctc cagggccgac cacggggcga actgcccgta
     2221 cgcctcgtcg accaccagca ggccgccgat gccctcgacc aggtcgagga cctcggtgac
     2281 ggtctcggcc gactcgacca tgccggtggg gttgttgggc gagcacaggt aggtgatggc
     2341 cgggcgcgcc tcggcgacga cccggcggac ctcgtcgagg tcgagcgcca ggtcggcgcc
     2401 gcgagcgccc tcggccaccg tcgacccggc gacccgggcg atctggctgt gcatggcgta
     2461 ggtgggctcg aacacggcga tggaccgccc cggcccgccg taggtgagcg acagcgtctg
     2521 gagcacctcg ttggacccgt tggccacgaa gacctgcccg gaacccacgc cgtggacggc
     2581 agcgaggcgg cgccgcagct cggtggcctg gcggtccggg taccggtgcc actcggtggc
     2641 cgccacct
//
LOCUS       DATMFS010000009         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_186358, whole genome shotgun sequence.
ACCESSION   DATMFS010000009 DATMFS010000000
VERSION     DATMFS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186358"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VNQ33_00160"
     CDS             complement(<1..515)
                     /locus_tag="VNQ33_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96534.1"
                     /translation="MAVDADLRDRARSWLAADPDPTTRDELDAVITAAEAGDEAAAAD
                     LADRFAADLDFGTAGLRGRLGAGPNRMNLAVVTRTANGIVSWLKGSGDPDAASKGVAV
                     CFDARHRSEDFAERSAAIFAAAGVRAYVLPGPLPTPVLAYAVSHLGAAAGVMVTASHN
                     PPQDNGYKVYDG"
     gene            complement(515..1348)
                     /locus_tag="VNQ33_00165"
     CDS             complement(515..1348)
                     /locus_tag="VNQ33_00165"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWJ96535.1"
                     /translation="MTHPDPVSHPFDAAVASAARLVEATGVDRHDVAIVLGSGWKPAA
                     DRMGDVAAELPMTELGGFPEASVQGHGTTVRSIANGSKRILAFMGRVHLYEGHHPNVV
                     AHGIRTAVRAGCSTVVLTNAAGGLRPGMWPGQPVLIADHLNMTGRTPLLGENDERIGP
                     RFPDMGEAYTPRLRELARSVDPTLEEAVYCGLLGPTYETPAEVRMVATLGGELVGMST
                     VLESIAAAHAGADLLAVSLVTNLAAGMADEVLAHDDVLAVAAESAERMGGLIAEIVGQ
                     L"
     gene            complement(1501..2508)
                     /locus_tag="VNQ33_00170"
     CDS             complement(1501..2508)
                     /locus_tag="VNQ33_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HWJ96536.1"
                     /translation="MSATSNHPAAFDVLGIGNALVDVLSTEEESFIEKMDLVKGSMTL
                     IDTERAEELYAAMGGDKTEMSGGSAANTLSGVASFGGRAAYIGRVKDDGLGKAFSHDL
                     NSLGVHFSADLATDGDPTGRCMIVVTPDGERTMNTYLGASATLCADHLDLDLIGSAKV
                     TFLEGYLFDRDDAKHAFHTAAAAAHAAGRKVSLTLSDSFCVDRHRDDFLTLVAEGVDI
                     LFANEEEIIKLYGVDTFDEGVDAVRGICDIAAVTCGRNGSVVVTKDELIQVEAHQVPK
                     RVDTTGAGDLYAAGFLYGWSQDRPLAECGRLGSIAAAAVIGHIGPRPGMSLAQTVSLL
                     D"
     gene            2639..>2985
                     /locus_tag="VNQ33_00175"
     CDS             2639..>2985
                     /locus_tag="VNQ33_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96537.1"
                     /translation="MIATRTRSAVAVVALSTAGLLAGCGSSADEPADTTKAGASTTTA
                     ADTSGTTSTPAETATTTAGGETTTTLSNEGRDTETSELDTLSDGDHYVYMAGLEKGTV
                     EGQAVQVLLVDPV"
BASE COUNT          428 a         1047 c         1108 g          402 t
ORIGIN      
        1 ccgtcgtaga ccttgtagcc gttgtcctgg ggcgggttgt ggctggccgt gaccatgacc
       61 ccggcggcgg ccccgaggtg cgacacggcg tacgccagca cgggcgtcgg gagcgggccg
      121 ggcaggacgt aggcgcggac cccggcggcg gcgaagatgg ccgcgctgcg ctcggcgaag
      181 tcctcggagc ggtggcgggc gtcgaagcac acggcgacgc ccttcgacgc cgcatcgggg
      241 tcgcccgacc ccttgagcca cgagacgatg ccgttggccg tccgagtgac caccgccagg
      301 ttcatgcggt tgggcccggc gccgaggcgg ccgcgcagcc cggccgtgcc gaagtcgaga
      361 tcggccgcga accggtcggc caggtcggcc gcggccgcct cgtcgcccgc ctccgcggcg
      421 gtgatcaccg cgtccagctc gtcgcgggtg gtcgggtccg ggtcggcggc gagccaggac
      481 cgggcccgat cccgcaggtc agcatcgacg gccatcagag ctggcccacg atctcggcga
      541 tgagcccgcc catgcgctcg gcggactcgg ctgcgacggc cagcacgtcg tcgtgggcca
      601 ggacctcgtc ggccatgccg gcggccaggt tggtcacgag cgacacggcc aacaggtcgg
      661 ccccggcgtg ggcggcggcg atggattcga gcaccgtgga catgccgacc agctccccgc
      721 cgagcgtcgc caccatccgc acctcggcgg gtgtctcgta ggtggggccg agcaggccgc
      781 agtagacggc ctcctccagg gtcgggtcga cggagcgggc cagctcccgg agccggggcg
      841 tgtacgcctc ccccatgtcg gggaaccgcg gcccgatgcg ctcgtcgttc tcgcccagca
      901 gcggcgtccg accggtcatg ttgaggtggt cggcgatgag cacgggctgg cccggccaca
      961 tccccggtcg caggccgccg gccgcgttgg tgagcaccac cgtcgagcat ccggctcgca
     1021 ccgcggtgcg gatgccgtgg gcgaccacgt tggggtggtg gccctcgtag aggtggaccc
     1081 ggcccatgaa cgcgaggatc cgcttgctgc cgttggcgat ggaccgcacc gtggtgccgt
     1141 ggccctgcac cgacgcctca gggaaaccgc cgagctcggt catcggcagc tcggcggcca
     1201 cgtcgcccat ccggtccgcg gcgggcttcc agcccgagcc caacacgatg gccacgtcgt
     1261 gccggtcgac gcccgtcgcc tccacaagcc gagcagcaga agccaccgca gcgtcgaacg
     1321 ggtggctgac cggatccggg tgcgtcaccc agccagtctg cccgaccccc gctgtttcgg
     1381 gtcgtctgag caccccacgg gtgcacgcgt gacccgaaat ggcatgccgc gccccgccac
     1441 gcaggtttcg ggtcgcctgg gcacctcagg ggtgcaccga tgacccgaaa tcgggtggtg
     1501 tcagtcgagg aggctgacgg tctgggcgag gctcatgccg gggcgggggc cgatgtggcc
     1561 gatgacggcg gcggcggcga tcgagcccag gcgaccgcac tcggcgaggg gccgatcctg
     1621 cgaccagccg tagaggaagc cggcggcgta gaggtcgccg gcgccggtgg tgtccacgcg
     1681 cttggggacc tggtgggcct cgacttggat gagctcgtct ttggtgacga cgaccgagcc
     1741 gttgcgcccg caggtgacgg cggcgatgtc gcagatgccg cgcacggcgt cgacgccctc
     1801 gtcgaacgtg tcgaccccgt agagcttgat gatctcctcc tcgttggcga agaggatgtc
     1861 gacgccctcg gcgacgagcg tcaagaagtc gtcgcggtgg cgatcgacgc agaagctgtc
     1921 cgacagcgtg agagagacct tgcgcccggc ggcgtgggcg gccgcggcgg ccgtgtggaa
     1981 cgcgtgcttg gcgtcgtcgc ggtcgaagag gtagccctcg aggaaggtga ccttggccga
     2041 gccgatgaga tcgaggtcca ggtggtcggc gcagagggtg gccgaggccc cgaggtaggt
     2101 gttcatggtg cgctcgccgt cgggcgtgac cacgatcatg cagcggccgg tggggtcgcc
     2161 gtcggtggcc aggtcggcgg agaagtggac gccgagcgag ttgagatcgt ggctgaacgc
     2221 cttgccgagg ccgtcgtctt tgacccggcc gatgtaggcc gcccgcccgc cgaacgaggc
     2281 gacgcccgag agcgtgttgg cggccgagcc gcccgacatc tcggtcttgt cgccacccat
     2341 ggcggcgtag agctcctcgg cccgctcggt gtcgatcagg gtcatcgacc ccttgacgag
     2401 atccatcttc tcgatgaacg actcctcctc ggtgctcagc acgtcgacga gggcgttgcc
     2461 gatgccgagg acgtcgaagg cggccgggtg gttcgaggta gcgctcacaa gggggagacc
     2521 ctaggcgccg cccgtccgcg ccctgaaccc gccgagcgcg ccgtcgccgg gccgagggcc
     2581 tccgagtagc gtggaccgac cgcccgtcgg gcaccgcacc accccctgga gctcttcgat
     2641 gatcgccacc cgtacccgca gcgccgtcgc cgtcgtcgcc ctctccacgg ccggtctgct
     2701 ggccggctgc ggatcgagcg cggacgagcc cgccgacacc accaaggccg gggcgagcac
     2761 caccacggcg gcggacacca gcgggaccac ctcgaccccc gccgagacgg ccaccacgac
     2821 ggccggcggc gagaccacca ccaccttgag caacgagggc cgcgacaccg agacgagcga
     2881 gctcgacacc ctctccgacg gcgaccacta cgtgtacatg gccggcctgg agaaggggac
     2941 ggtcgaaggc caagccgtgc aggtcctcct cgtggacccc gtcga
//
LOCUS       DATMFS010000010         6736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_116792, whole genome shotgun sequence.
ACCESSION   DATMFS010000010 DATMFS010000000
VERSION     DATMFS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116792"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VNQ33_00180"
     CDS             complement(<1..992)
                     /locus_tag="VNQ33_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ96538.1"
                     /translation="MQPSDDGTTGGRGPARPGVLGFVGIHAGSRAQARSQSETLAALF
                     RQEGNDVRAAGDAGNPIVRALHHVAALAAWRDVDVVVVDVFSGRSFTMADYGSLLGRR
                     LWGRKVVLFLHGGNLPEYGPAHRARVERVLRRADQILAPSDYLADVFRSWGFDVRVIP
                     NVLEIEGYAYEPRQQARPALMWMRTFQDDYDPLLAVEVLHRVAAVHPEATLTMGGADH
                     GLLDATRARAAELGVAERVSFAGFLDGAGKARAFADHDVFLNTNRVDNMPVSVLEAAA
                     CGLVPVATAVGGIPALLTDGVDSRVVPAADAGAMAGAVLELLREPAAFGRLSAAA"
     gene            1105..1905
                     /locus_tag="VNQ33_00185"
     CDS             1105..1905
                     /locus_tag="VNQ33_00185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HWJ96539.1"
                     /translation="MPGEDAYKKRGIRGRVWKFYRHHVRDHISDFVVRQRLKLLWMGW
                     GYWRVLAPSVLPVHRRIWLVARFLRVDWKVLHAHKPIEIGYMLRDLASRRANPGEVVV
                     EAGCWQGGSTAKFSLMCRELGYGLEVYDSFEGVSAHDVRAGETDFSGTYAAAEELVRR
                     NVAELGAIEVCTFHKGWFSDTLRNDGVGHPTRMLYIDCDIIDGTTDALTGGLPELVDD
                     SLVYSQDFHIPAVREYLEDPATWAAFDRGPARLTPLAWNLVRVEVPPA"
     gene            1919..3295
                     /locus_tag="VNQ33_00190"
     CDS             1919..3295
                     /locus_tag="VNQ33_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96540.1"
                     /translation="MDLHAIYERLPPPLRSAAASARGLQLARSRYGPETERLVDEAVE
                     RDHWSAEQWQAWREERLAHVLHRARTQVPWYRDHWDGRQRRGDTASPDDLANWPILTK
                     AELRGDPRAFVADDRPTGKLIADHTSGTTGTPLTLWFDQDGVRAWYALFEARVRRWHG
                     VDRSAPVAMLGGQAVVPASVASPPFWITNRPMHQLYLSTFHIRADRAAAYAQAIRRHG
                     TRYLLGYTSALTALALAMAEAGVEPPRVQVAITNAEPCDDTDRALIEAAFGAPVRMTY
                     GMAEVVACATECPSGTLHEMPDVGWLELDADSPPVDGEAPGTGELVATGLVNDAMPLV
                     RYRVGDRLTLPSPTTCTCGRTLPEVASVTGRTDDVVVTADGRRVGRLDHIFKADLPIH
                     EAQVVQASLTRIRILVVPAEGWSERDRREVAERAKERIGDVDVEVIDCDRIPRAANGK
                     FRAVVSQL"
     gene            complement(3305..3976)
                     /locus_tag="VNQ33_00195"
     CDS             complement(3305..3976)
                     /locus_tag="VNQ33_00195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ96541.1"
                     /translation="MSGQRGGGATPDDGLELRTLGPGDLADVAALHVVVFRDSVLSEL
                     GPEAVRRYYRWQLTGPHDVEAFGLFAGDDLVGFLVGGRFRGSMIGFIKRETPFLARCV
                     LRHPTLVIGARGRSALRTGIRLLVRPAEPTVVERPERVPDKSFGVLSVAIDPSVQRSG
                     MGSLLLDEADRRAAAMGMERLHLTLDPQNWGAAEFYRGQGWRRLGLPGDTDHQWLMGK
                     ELPDR"
     gene            complement(3973..5187)
                     /locus_tag="VNQ33_00200"
     CDS             complement(3973..5187)
                     /locus_tag="VNQ33_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ96542.1"
                     /translation="MTSRRPAPRSLAVIADLPHHRDAAGRLYGLEPVVAQLDEWAALF
                     DRLVICSPVLPGAPPAGFAPYRSSGIEMVDLPRGGGNTLGAKLAMIPLIPGWAWRTRR
                     VARRVDAVHLRCPSNIGLVAVFSTWRAARYRSAMYAGVWRHYDGEPRFFRLQRELLRG
                     RWFGGPVSVYAPADPRRPHLEPSFSPSFTHAAWEAAAPTAEAKRDSVSARPDAGVWRL
                     AVVGRLTPNKNQQAAVRAVADLAASGLDVELDVLGDGPERGRLELLARDLGLGDRVRF
                     RGMVPFDEVTEVFAAADLQLLPSRQEGYGKVLLEGMVQGTVPLLSPGPAADGIAGHGS
                     RGLLIDPHAPATIAAAVRSLIDDRPRWLGMIDDARAYTAEHTLEVFRTRLRAMLERQW
                     DVDLAGVVASDQ"
     gene            complement(5184..>6736)
                     /locus_tag="VNQ33_00205"
     CDS             complement(5184..>6736)
                     /locus_tag="VNQ33_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWJ96543.1"
                     /translation="GRPAAASAAVRRHLDSGGAGAWLLFWDPVLGDPPVDVVEAFASG
                     RVGDAWHGGLDLGMSGLPAEHDYIHPSHPFVLDPPSDVAGICWRLSLGAALIRTEAYT
                     AAGGIDPAFHGVTGAGLALGRRLTERGGIVRNGPGLVGAAGAHVEPLDEHDRFLFLNR
                     TFGRKWVRYAAVRRAMATRRPGRTYRGWRAAERTHAEHPAPPAELIERAPVPFDEGAT
                     VSVVLPTLGRYEMLRPLLAQLCDQTVTPLEVLVIDQNDPDKRDHDLYASFADRGVRVI
                     WQEVRGQWISRNAAVQEAKGEWIAFIDDDSEIEPDWIAAHLEAIARYGADLSTGASLA
                     VVGAPVPDSYAYYGVADQWDSGNGMCRRSLFAEMGLFDQQFDRQRRGDAEFGLRVQLH
                     GGLVVHVPRGIRIHLKAEEGGLRTYGQWDGFRNKDRSGPLPVPSMLYYMDRYHTPRQR
                     REDLMLGLVHGIVPYHLKRRTSPSAFAGFALAEVVHLPSTVRRVRRSRELAAAMVAQG
                     PSIPVLDA"
BASE COUNT          935 a         2541 c         2436 g          824 t
ORIGIN      
        1 gccgcggccg acaggcggcc gaaggcggcc ggttcgcgca gcagctcgag cacggcgccg
       61 gccatggcac cggcgtcggc ggcgggcacg acccggctgt cgacgccgtc ggtgagcagg
      121 gccgggatcc cgcccaccgc cgtcgccacc ggtaccagac cgcaggccgc cgcctccagg
      181 acgctgaccg gcatgttgtc cacccggttc gtgttcaaga acacgtcgtg gtcggcgaag
      241 gcgcgggcct tgcccgcgcc gtcgaggaac ccggcgaacg acacccgctc ggcgacgccc
      301 agctcggcgg cccgagcgcg ggtggcgtcg agcagcccgt gatcggcacc gcccatggtg
      361 agggtggcct cggggtggac ggcggccacg cggtgcagca cctcgacggc gagcagcggg
      421 tcgtagtcgt cctggaacgt gcgcatccac atcagcgccg gccgggcctg ctggcgcggc
      481 tcgtacgcgt agccctcgat ctcgagcacg ttcgggatga cgcgcacgtc gaagccccag
      541 ctccggaaca cgtcggcgag gtagtccgac ggcgccagga tctgatcggc ccggcggagg
      601 acccgctcga cgcgggctcg gtgcgcgggc ccgtactcgg ggaggttgcc accgtgcagg
      661 aacagcacca ccttgcgccc ccagaggcgc cggccgagca gcgatccgta gtcggccatc
      721 gtgaacgacc gtccggagaa gacgtcgacg acgacgacgt cgacgtcccg ccaggccgcg
      781 agcgcggcca cgtggtgcag ggcccggacg atcgggttgc ccgcatcgcc agccgcacgg
      841 acgtcgttcc cctcctggcg gaacagcgcg gccagcgtct cgctctggct gcgggcctgg
      901 gcccgcgacc cggcgtggat gcccacgaac ccgaggacgc caggccgagc cggccccctg
      961 cctcctgtgg tcccgtcgtc cgacggctgc atgtcgcgcc tccctgaccg gtcgctccga
     1021 ccggtcccga catcatggcc gaccgggtcc ggcggcccgg gacgctcgtc gggggcccga
     1081 gatggccctg gctacactcc gcctgtgcct ggggaagacg cctacaagaa gcgcgggatc
     1141 cgaggacggg tgtggaagtt ctaccgccac cacgtccgcg accacatcag cgacttcgtg
     1201 gtgcgccagc gcctcaagct gctctggatg gggtggggct actggcgggt cctcgcgccg
     1261 tcggtgctcc cggtccaccg ccgcatctgg ctcgtcgccc ggttcctccg ggtcgactgg
     1321 aaggtgctcc acgcccacaa gccgatcgag atcgggtaca tgctccggga cctcgcctcc
     1381 cgccgggcga accccggcga ggtcgtcgtc gaggccggct gctggcaggg cggctccacc
     1441 gccaagttca gcctcatgtg ccgggagctg ggctacggcc tggaggtcta cgactccttc
     1501 gagggggtct cggcccacga cgtccgggcc ggtgagacgg acttctccgg cacctacgcc
     1561 gcggccgagg agctggtgcg ccgcaacgtc gccgagctcg gcgccatcga ggtctgcacc
     1621 ttccacaagg gctggttctc cgacacgctc cgcaacgacg gcgtgggcca cccgacgcgg
     1681 atgctctaca tcgactgcga catcatcgac ggcaccaccg acgcgctcac cggcggcctc
     1741 cccgagctcg tcgacgacag cctcgtctac tcgcaggact tccacatccc cgcggtgcgc
     1801 gagtacctgg aggacccggc cacctgggcg gccttcgacc ggggcccggc gcggctcacc
     1861 ccgttggcct ggaacctggt gcgggtcgag gtcccgccgg cctgaaccgg cccgatcgat
     1921 ggacctccac gcgatctacg agcggcttcc cccgccgctc cgctcggctg ctgccagcgc
     1981 acgcggcctg cagctcgccc gctcccgcta cggccccgag accgagcggc tcgtcgacga
     2041 ggccgtggag cgcgaccact ggtcggccga gcagtggcag gcctggcgcg aggagcgcct
     2101 ggcccacgtc ctgcaccgcg cccgcacgca ggtgccctgg taccgcgacc actgggacgg
     2161 ccggcagcgg cgcggcgaca ccgcatcgcc cgacgacctc gccaactggc cgatcctcac
     2221 caaagccgag ctgcgtggcg acccccgtgc cttcgtggcc gacgaccggc ccacggggaa
     2281 gctcatcgcc gaccacacca gcggcaccac cggtacgccg ctcaccctgt ggttcgacca
     2341 ggacggcgtc cgggcctggt acgcgctgtt cgaggcgcgg gtccgccgct ggcacggcgt
     2401 cgaccggagc gcgccggtgg ccatgctcgg aggccaggcc gtggtgccgg catcggtcgc
     2461 ctcgccgccg ttttggatca ccaaccggcc gatgcaccag ctctacctgt cgacgttcca
     2521 catccgcgcc gaccgggccg ccgcctacgc ccaggccatc cggcgccacg gcacccgcta
     2581 cctgctcggg tacacgtcgg cgctcacggc gctcgccctg gcgatggccg aggccggcgt
     2641 cgagccgccc cgggtccagg tggccatcac caacgcggag ccctgcgatg acaccgatcg
     2701 ggcgctgatc gaggcggcgt tcggtgcccc cgtccggatg acctacggca tggccgaggt
     2761 cgtggcctgc gccacggagt gcccgagcgg gacgctccac gagatgcccg acgtcggctg
     2821 gctggagctc gacgccgaca gcccgccggt cgacggcgag gcccccggaa cgggcgagct
     2881 cgtcgcgacc ggcctcgtga acgacgccat gcccctcgtg cgctaccggg tgggcgaccg
     2941 gctcaccctg ccctcgccca ccacgtgcac gtgcggccgc acccttcccg aggtcgccag
     3001 cgtgacgggc cggaccgacg acgtcgtggt gaccgccgac ggccggcggg tcggccgcct
     3061 cgatcacatc ttcaaggcgg acctcccgat ccacgaggcc caggtggtcc aggccagcct
     3121 gacgcgcatc cgcatcctgg tcgtgccggc cgagggatgg tccgagcgcg accgccgcga
     3181 ggtggccgaa cgggccaagg agcgcatcgg cgacgtcgac gtcgaggtca tcgactgcga
     3241 ccgcatcccc cgcgccgcca acggcaagtt ccgcgccgtc gtctcccagc tctgatcgag
     3301 ccgctcagcg gtcgggcagc tccttgccca tgagccactg gtggtcggtg tcacccggca
     3361 agccgaggcg gcgccagccc tggccccggt agaactcggc cgcgccccag ttctgcggat
     3421 cgagggtgag gtgcagccgc tccatcccca tcgccgcggc ccggcggtcg gcttcgtcca
     3481 gcagcaggct gcccatcccc gaccgctgca cgctcggatc gatggcgacg gagaggaccc
     3541 cgaacgactt gtccggcacc cgctcgggcc gctcgacgac ggtgggctcg gccggccgga
     3601 cgaggagccg gatgcccgtc cggagcgcgg accggccgcg ggcgccgatg acgagggtcg
     3661 ggtggcggag cacgcaccgg gcgaggaagg gcgtctcccg cttgatgaac ccgatcatcg
     3721 agccacggaa ccgtcccccg acgaggaacc cgacgaggtc atctccggcg aagagcccga
     3781 acgcctcgac gtcgtggggc ccggtgagct gccagcggta gtagcggcgc accgcctccg
     3841 gccccagctc ggacagcacc gagtcgcgga acaccacgac gtgcagggcg gcgacgtcgg
     3901 ccaggtcgcc gggtcccagg gtccggagct ccagcccgtc gtccggcgtc gcgccgcctc
     3961 cccgctgccc gctcactggt ccgaagctac gacgccggcc aggtcgacgt cccattgacg
     4021 ctcgagcatc gcccgcagcc gcgtccggaa cacctcgagc gtgtgctcgg ccgtgtacgc
     4081 ccgggcgtcg tcgatcatgc ccagccaacg ggggcggtcg tcgatcagcg accggacggc
     4141 agcggcgatc gtcgccgggg cgtgggggtc gatgagcagc ccgcggctcc cgtgcccggc
     4201 gatgccgtcg gccgccggcc ccggcgagag cagcggcacc gtgccctgga ccatcccctc
     4261 gagcagcacc ttgccgtaac cctcctgccg gctcggcagg agctggaggt cggccgccgc
     4321 gaacacctcc gtcacctcgt cgaacggcac catgccccgg aaccggaccc ggtcgccgag
     4381 ccccaggtcg cgcgccagca gctcgaggcg gccccgctcc gggccgtcgc cgaggacgtc
     4441 gagctccacg tcgaggcccg acgccgccag gtcggcgacg gcccggaccg ccgcctgctg
     4501 gttcttgttg ggggtcaggc gcccgaccac cgcgaggcgc cacaccccgg cgtcggggcg
     4561 ggcgctgacg gagtcccgct tcgcctcggc cgtcggagcg gccgcctccc acgccgcgtg
     4621 cgtgaacgac gggctgaacg acggttcgag gtgcggccgc cgcggatcgg ccggcgcgta
     4681 gacgctcacc ggccccccga accaccggcc gcgcagcagc tcgcgctgga gccggaagaa
     4741 gcggggctcg ccgtcgtagt ggcgccacac cccggcgtac atcgccgagc ggtaccgggc
     4801 cgcccgccac gtggagaaga cggcgaccag cccgatgttg gacggacagc ggaggtggac
     4861 ggcgtcgacg cggcgggcga cccgccgcgt gcgccacgcc cagcccggga tcagcgggat
     4921 catggccagc ttggcgccca gcgtgttccc cccgccgcgc gggaggtcga ccatctcgat
     4981 ccccgaggac cggtagggcg cgaaccccgc cggcggcgcg ccgggaagca ccgggctgca
     5041 gatcacgagg cggtcgaaga gggccgccca ctcgtcgagc tgggccacga ccggttcgag
     5101 gccgtagagg cgcccggcgg catcccggtg gtgcgggagg tcggcgatga cggcgagcga
     5161 acgaggcgcc gggcggcgcg aggtcacgcg tcgagcaccg ggatcgacgg cccctgcgcc
     5221 accatcgccg ccgccagctc ccgcgaccgg cgcacgcggc gcacggtgct cgggaggtgc
     5281 acgacctcgg cgagggcgaa cccggcgaac gcgctcggcg aggtccggcg cttgaggtgg
     5341 taggggacga tgccgtggac caggccgagc atgaggtcct cgcggcgctg gcgaggcgtg
     5401 tggtaccggt ccatgtagta gagcatcgac ggcaccggga gcggtccgga gcggtccttg
     5461 ttgcggaacc cgtcccactg gccgtaggtg cgcaacccgc cctcttcggc cttgaggtgg
     5521 atccggatgc cgcggggcac gtggacgacc aggccgccgt gcagctggac ccgcaggccg
     5581 aactcggcat cgccccgccg ctggcggtcg aactgctggt cgaacaggcc catctcggcg
     5641 aagagcgacc gccggcacat gccgttgccg gagtcccact ggtccgcgac gccgtagtag
     5701 gcgtaggagt ccggcaccgg ggcgccgacg acggcgagcg atgcgcccgt cgagaggtcg
     5761 gcgccgtagc gggcgatggc ctcgaggtgg gcggcgatcc agtccggctc gatctcggag
     5821 tcgtcgtcga tgaaggcgat ccactccccc ttcgcctcct ggacggcggc gttgcgcgag
     5881 atccactgcc cgcgcacctc ctgccagatg acccggacgc cgcggtcggc gaacgaggcg
     5941 tacaggtcgt ggtcgcgctt gtccggatcg ttctggtcga tgaccaggac ctcgagcggc
     6001 gtcacggtct ggtcgcagag ctgggcgagc agcgggcgga gcatctcgta gcggcccagg
     6061 gtgggcagca cgacgctcac cgtcgcgccc tcgtcgaacg ggaccggtgc ccgctcgatc
     6121 agctcggcgg gtggggccgg gtgctcggcg tgggtgcgct cggcggcccg ccacccccgg
     6181 taggtccgcc ccgggcggcg ggtcgccatc gcccgccgga cggcggcgta gcgcacccac
     6241 ttgcggccga acgtccggtt caggaacaag aagcggtcgt gctcgtcgag gggctcgacg
     6301 tgggcgcccg ccgcgccgac gaggcccggg ccgttccgca cgatgccgcc ccgctcggtc
     6361 aaccgccggc cgagggcgag cccggccccc gtgacgccgt ggaacgccgg gtcgatgccg
     6421 cccgcagcgg tgtacgcctc cgtgcggatc agggcggcgc cgagcgacag ccgccagcag
     6481 atgcccgcga cgtcgctcgg cgggtcgagc acgaaggggt gggacgggtg gatgtagtcg
     6541 tgctcggcgg ggagccccga catccccagg tcgagtccgc cgtgccaggc atcgccgacc
     6601 cgtccggatg caaacgcctc gacgacgtcg accggcgggt cgccgagcac cggatcccag
     6661 aacagcagcc aggcccccgc tccgccggaa tcgaggtgcc gtcggaccgc ggccgatgcc
     6721 gcagcaggac ggcccg
//
LOCUS       DATMFS010000011         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_93262, whole genome shotgun sequence.
ACCESSION   DATMFS010000011 DATMFS010000000
VERSION     DATMFS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93262"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VNQ33_00210"
     CDS             <1..248
                     /locus_tag="VNQ33_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96544.1"
                     /translation="SPGEYLVETDPSPANIAALTSWLAEQDLPLADIRAGRQTLEDVF
                     LRMTAITGEVPAVRIDTRSKDSAKARTERLRGRRRSA"
     gene            245..1003
                     /locus_tag="VNQ33_00215"
     CDS             245..1003
                     /locus_tag="VNQ33_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96545.1"
                     /translation="MTAPTVPVAASSGQAWKAQTRIELLLTLRRGESVLLTFLIPVVL
                     LGFFANVDVLPTGDGDPIDFLLPGVLALAIMSTALVSVAIATGFERQMGVLKRLGTTP
                     LTRRQLLAAKTTAVVAIEALQIIVLLAEGFLLGFRFSAPSLGVALVAAVLATVAFAGI
                     GLLMAGTLPALTTLAAANGVYLLLLLVSGMIFPIDELPSGMRSVAELLPSTALAQVFH
                     GALGDGTVPGSALVVLAVWAAAAPLAAARWFRWE"
     gene            complement(1132..1515)
                     /locus_tag="VNQ33_00220"
     CDS             complement(1132..1515)
                     /locus_tag="VNQ33_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWJ96546.1"
                     /translation="MSEISVPVAGDAHPDLEHHPEEHASDRQFVKVFLGLVVVTAVEV
                     AWAYAPWADDHAGRLATALYWGGLMLMMSFKFFIIASYFMHLRFDSKVLTRVFYAGVV
                     LAVAVYLIVLCTFQLFSNTTPGFAP"
     gene            complement(1526..2143)
                     /locus_tag="VNQ33_00225"
     CDS             complement(1526..2143)
                     /locus_tag="VNQ33_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HWJ96547.1"
                     /translation="MTDLTAALKEELVGHDHEHVTSTGISNEKLAMWVFLGSECLLFG
                     GLISTFILYKTRAADGQTPHQLYDILFTSISSFVLLMSSLTMVLAVSAVQRADLRAMR
                     GWLLTTALLGVVFISGQVYEFTEFLGEGYGFTHSPAWSAFYTLTGFHGVHVTLGIVML
                     LSTAALSLRGHVGSHNPESVEIVGLYWHFVDVVWVVIFTVVYLIK"
BASE COUNT          334 a          795 c          771 g          349 t
ORIGIN      
        1 cctcaccggg cgagtacctc gtcgagaccg acccgtcgcc cgccaacatc gccgcgctca
       61 ccagctggct ggccgagcag gacctgccgc tcgccgacat ccgcgccggc cgccagaccc
      121 tcgaggacgt cttcttgcgc atgaccgcca tcacgggcga ggtaccggcc gtccgcatcg
      181 acacccgctc gaaggactcc gccaaggccc gcaccgagcg gctccggggc cggcggcgat
      241 cggcgtgacc gcccccacgg tccccgtggc cgcctcgtcg ggccaggcct ggaaggcgca
      301 gacccgcatc gagctcctgc tcacgctccg ccggggcgag tcggtcctgt tgaccttcct
      361 gatcccggtc gtcctcctcg gcttcttcgc caacgtcgac gtgctgccca ccggcgacgg
      421 cgaccccatc gacttcctgc tgccgggtgt gctcgccctg gcgatcatgt cgacggccct
      481 ggtgtcggtg gccatcgcca ccggcttcga gcgccagatg ggcgtgctga agcgcctcgg
      541 gaccaccccg ctcacccgcc ggcagctcct ggcggcgaag accaccgccg tcgtggccat
      601 cgaagcgctc cagatcatcg tgctgctggc ggagggcttc ctcctcgggt tccgcttctc
      661 ggccccgtcg ctgggcgtcg ccctggtcgc cgccgtgctc gccaccgtcg ccttcgccgg
      721 catcggcctg ctcatggcgg gcacgctccc ggccctcacc accctcgcgg ctgcgaacgg
      781 cgtctacctc ctgctcctgc tggtcagcgg catgatcttc cccatcgacg agctcccctc
      841 ggggatgcgg tcggtggcgg agctgctccc gtccaccgcc ctggcccagg tcttccacgg
      901 cgcgctgggc gacggcacgg tccccggctc ggccctcgtg gtcctggcgg tctgggcggc
      961 cgccgccccc ctcgccgccg cccgctggtt ccgctgggag taggggctcc accgggctcg
     1021 tgcccgtcgg ctcggtcctc ttcggtcccg tcggctggcc gagccctgga cgcgatcgag
     1081 gcgccccgga gggcgcctcg atgcacagag atcagcttcg agcggtcacc ctcagggcgc
     1141 gaaccccggg gtggtgttgc tgaagagctg gaaggtgcac aagacgatca ggtacaccgc
     1201 gaccgccagt accacgccgg cgtagaacac ccgggtgagc accttgctgt cgaagcgcag
     1261 gtgcatgaag tagctggcga tgatgaagaa cttgaagctc atcatcagca tcaggccgcc
     1321 ccagtagagg gcggtggcga ggcggccggc gtggtcgtcg gcccacgggg cgtaggccca
     1381 ggcgacctcg acggccgtca cgaccaccag gccgaggaag accttgacga actgccggtc
     1441 cgaggcgtgc tcctccgggt ggtgctcgag gtcggggtgg gcgtccccgg cgacgggaac
     1501 ggagatctcg ctcatgtcca gtcccttact tgatgaggta gacgacggtg aagatgacga
     1561 cccagacgac gtcgacgaag tgccagtaca ggcccacgat ctcgacggat tccgggttgt
     1621 gggatccgac gtggccgcgc agcgagagcg ctgcggtgga cagcagcatc acgatgccga
     1681 gcgtcacgtg gacgccgtgg aagccggtca gcgtgtagaa ggcggaccag gccgggctgt
     1741 gggtgaagcc gtagccctcg cccaggaact cggtgaactc gtagacctgg ccggagatga
     1801 acaccacgcc caggagggcg gtggtgagca gccagccgcg catggcccgc aggtcggcgc
     1861 gctgcaccgc ggagacggcg agcaccatcg tgagcgagct catgaggagc acgaacgacg
     1921 agatcgacgt gaagaggatg tcgtagagct ggtgcggggt ctggccgtca gcggcccggg
     1981 tcttgtacag gatgaacgtg gagatgaggc cgccgaagag caggcactcg gatcccagga
     2041 agacccacat cgccagcttc tcgttggaga tgccggtcga ggtgacgtgc tcgtggtcgt
     2101 gcccgaccag ttcttccttc agcgcagcag tgagatcagt caacgagcgc agcctcctcg
     2161 gtcggagcgg catcgccgct cggctcatcg gcatcgtcgg ccgcggaggc ctcgtcaccg
     2221 ctcgggtcgt cgtcgtgatc gtcttcgtg
//
LOCUS       DATMFS010000012         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_799, whole genome shotgun sequence.
ACCESSION   DATMFS010000012 DATMFS010000000
VERSION     DATMFS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VNQ33_00230"
     CDS             complement(<1..200)
                     /locus_tag="VNQ33_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96548.1"
                     /translation="MPDAPLPPGRPVAPGAVPNPAAARPGVRSSEPADVEPADEMPLS
                     RPARGALKAAEWLPDWAGSIRFR"
     gene            complement(193..921)
                     /locus_tag="VNQ33_00235"
     CDS             complement(193..921)
                     /locus_tag="VNQ33_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ96549.1"
                     /translation="MRILVVEDESELAEAIATGLRREGYAVDVANDVAGAVDRLVVSP
                     YDLVTLDLNLPDGDGLDLARRVREDPAFAPVEGETAPRILMLTARDGVGERVAGLDQG
                     ADDYLVKPFAFAELSARVRSLMRRDAGRSGATITVGELELDTARFEAARRGRLLDLTA
                     KEFALLRYFMTHSGEVLSQERLLEHVWDENADPLTNTVRVTVGTLRRKLTRDDDAEAP
                     IETVIGAGYRLRPDLLASGPAADA"
     gene            1078..2334
                     /locus_tag="VNQ33_00240"
     CDS             1078..2334
                     /locus_tag="VNQ33_00240"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWJ96550.1"
                     /translation="MSDQPMGAQNPALAAERLEQTLFEVKKVIVGQDRTVERLLICLL
                     ARGHALLEGVPGLAKTLAAETIARVVGGSFTRIQFTPDLLPADVMGTRIYRSDSQRFD
                     VELGPVFANFVLADEINRAPAKVQSALLEVMAEHQVSIGGNTFPVPEPFIVLATQNPI
                     EQEGVYPLPEAQRDRFLMKIVVDYPTPSEEVEIVQRMGVAPPEPTESLTIDALAVLQD
                     EADKIFVAREVVDYAVNLVFATREPERFGMADLREFLQYGASPRASLGLVAAGRALAL
                     LRGRAYVLPQDVYDVAPDIFRHRFVLSYEALARDITVNDVLARILATVPAPRVAPSQD
                     HTSHRVPSAPTGWSQTAPAASAPAAALAPMPSVTPSAPAPTAWDEASAATPAPWASPP
                     ATAQPPSEATGTENPSSGWGAPGEAR"
BASE COUNT          349 a          961 c          829 g          330 t
ORIGIN      
        1 cggaagcgga tggacccggc ccagtccggg agccactcgg cggccttcag cgcgccgcgc
       61 gccggccgcg agagcggcat ctcgtcagcg ggctcgacat cggcgggctc cgagctgcgg
      121 acgcccgggc gcgccgcggc cgggttcggg acggctcccg gagcgacagg gcgcccggga
      181 ggcagcgggg catcaggcat cagcggccgg gcccgatgcc aacaggtcgg gccgcaagcg
      241 gtagccggca ccgatgacgg tctcgatcgg cgcctcggca tcgtcgtcac gggtgagctt
      301 gcggcgcaac gtgccgacgg tgacccgcac ggtgttggtg aggggatctg cgttctcgtc
      361 ccagacgtgc tcgagcaggc gctcttggct gaggacctcg ccgctgtgcg tcatgaagta
      421 gcgcagcaag gcgaactcct tggcggtgag gtcgaggagc cggcctcgac gcgccgcctc
      481 gaagcgggcc gtgtcgagct cgagctcccc caccgtgatc gtcgccccgc tgcgcccggc
      541 gtctcgccgc atcaggctgc gcacgcgcgc cgagagctcg gcgaaggcga agggcttcac
      601 gaggtagtcg tcggcgccct ggtcgagacc cgccacccgc tcccccacgc cgtcgcgcgc
      661 cgtcagcatc aagatccgcg gcgccgtctc cccctcgacc ggcgcgaacg ccgggtcctc
      721 gcgcacccgg cgcgccaggt cgagcccgtc gccgtcgggg aggttcaagt ccagcgtgac
      781 caggtcgtac ggggacacca cgaggcggtc gaccgcgccg gccacgtcgt tggcgacatc
      841 gacggcgtag ccctcccgcc gcaggcccgt ggcgatggcc tcggccagct cgctctcgtc
      901 ttcgaccacc aggatgcgca cggcggaaac gtaccggtcc aggaggaaac ctcgcagaaa
      961 cgtcccacct gggccgattc gcttgcccgc agccccgtgc gaccgacagg gctttacgct
     1021 gtccccacgc gcccggcgtc gctgggcctc cgagccgtcc tctagaggag catctccatg
     1081 tccgatcagc cgatgggcgc ccagaacccg gcgctggctg ccgaacgcct cgagcagacc
     1141 ctcttcgagg tcaagaaggt catcgtcggc caggaccgca cggtggagcg cctgctgatc
     1201 tgcctgctcg cacgaggcca tgccctgctc gaaggcgtgc cggggctggc caagacgctg
     1261 gccgcggaga ccatcgcccg cgtggtcggc ggctcgttca cccgcatcca gttcacgccc
     1321 gatctgctgc ccgccgacgt gatgggcacc cgcatctacc ggagcgactc gcagcgcttc
     1381 gacgtcgagc tcggcccggt cttcgccaac ttcgtcctcg ccgacgagat caaccgcgcg
     1441 cccgccaagg tgcagtccgc gctgctcgag gtcatggccg agcaccaggt gtccatcggc
     1501 ggcaacacgt tcccggtgcc cgagcccttc atcgtgctcg ccacccagaa ccccatcgag
     1561 caggagggcg tctacccgct ccccgaggcg cagcgcgacc gcttcttgat gaagatcgtg
     1621 gtcgactacc cgacgccgtc ggaggaggtc gagatcgtgc agcgcatggg cgtggccccg
     1681 ccggagccca cggagtcgct gaccatcgac gccctcgccg tgctccaaga cgaggccgac
     1741 aagatcttcg tcgcccgaga ggtcgtcgac tacgccgtga acctcgtctt cgccacccgc
     1801 gagcccgagc gcttcggcat ggccgacctc cgtgagttcc tccagtacgg cgccagcccc
     1861 cgtgccagcc tcggcctcgt cgccgcgggc cgcgccctcg ccctgctgcg cggtcgggcc
     1921 tacgtgctgc cccaggacgt ctacgacgtg gcacccgaca tcttccgcca ccgcttcgtc
     1981 ctgtcgtacg aggcactggc ccgtgacatc acggtgaacg acgtcttggc ccggatcctg
     2041 gccaccgtgc cggcaccgcg ggtggcaccg tcgcaggacc acacctcgca ccgcgttccc
     2101 agcgccccga cgggctggag ccagacggcg cccgcggcat cggcccccgc cgccgccctg
     2161 gcgccgatgc cctccgtcac cccctcggcg cccgcgccca ccgcgtggga cgaggcatcg
     2221 gccgccaccc cggccccgtg ggcctcgccg ccggccacgg cccagcctcc gtcggaggcg
     2281 accgggaccg agaacccgag cagcggatgg ggcgccccgg gagaggcgcg ctgacccgtg
     2341 gcccagcccg cgaacgttcc accccgcacg gcgaagaccg gccctgacga ggtcctgcgc
     2401 cgcctcgacc tggccgtgag ccacaagctc gacggcctgt tgcacggcga gtaccagggc
     2461 ctggttccc
//
LOCUS       DATMFS010000013         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_23752, whole genome shotgun sequence.
ACCESSION   DATMFS010000013 DATMFS010000000
VERSION     DATMFS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23752"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..997
                     /locus_tag="VNQ33_00245"
     CDS             47..997
                     /locus_tag="VNQ33_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ96551.1"
                     /translation="MLASTVVPPAPIRAEADGLTPYPGARRPDRRRTVDRGGIALCVW
                     EWGDEDAPPILFAHGGFDFAGTYDTLAPLVADQGWRVVSWDQRGHGDSQHGHLYSWDA
                     DLRDAAAVLDSTSDGAIPILGHSKGGALMLQLADALPHRVSHVVNLDGLPSARSWPDV
                     PDHHRTRLLNGELAGWLDHRRVAADKIRRPGTIEELAERRGRMNPRMDPDWLRYLVPI
                     GAYEEADGWRWKIDASMRMGGFGPWRPEWSMSRLPSLGMPVLCVLGLDIEVMGWGTLP
                     EDVVPNLPAGARFVPLDGVGHFVHIEKPAVVADLTLELIS"
     gene            994..1701
                     /locus_tag="VNQ33_00250"
     CDS             994..1701
                     /locus_tag="VNQ33_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ96552.1"
                     /translation="MSTVTLLTHVKVQLALHELRPGTGRPLLLLHGLGEHTPAAVPAS
                     ASAWPGPVYGLDLTGHGASSRAPGGGYTAEILMADVDAALAHLGPVTIVGRGLGAYVA
                     MLIAGARPELVVGAVLCDGPGLSGGGPAPHSPSVVAPTAAAVAAATPESGLPDPFALV
                     ELARDVRPEDYATTYARQALQFSGLDAPLAIAAVTRPPWLAAVAAEPGVVAEPVAAAL
                     ERYAAMPMPTPTPTPTT"
     gene            complement(1744..>2787)
                     /locus_tag="VNQ33_00255"
     CDS             complement(1744..>2787)
                     /locus_tag="VNQ33_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWJ96553.1"
                     /translation="PLPGDLGLVAARVEARVTRLLDDETERWEAVEADLLHPLEALRA
                     LVMAGGKRLRPVFCHWGFVAAGGDPDDARVVDAGAAFELLQAFALIHDDVMDGSATRR
                     GTPAVHRRFADRHAAEAWAGESRRFGEGVAILVGDLALVYSDLLLPSGPPELAALWHE
                     LRIELNIGQYLDLAGTATGGVDRAGARRIARLKSGRYTIERPLQVGAVLAGNPGLAGA
                     LSQYGDPLGEAFQLRDDVLGVFGLTQRTGKPVGDDLREGKPTLMLAVVHERADGAQRD
                     LLARVGDPDLGEQEVADLQALFVSTGALDVAEAEIAALAGRAVTALPALRLDPHADLA
                     LRDLAAFVVARDA"
BASE COUNT          348 a         1053 c         1026 g          360 t
ORIGIN      
        1 gctcggactt gaccggcggg tcaagtccga gcggtagcct cagcggatgc tcgcttcgac
       61 cgtggtcccg ccggcgccga tccgggccga ggccgatggc ctgaccccgt acccgggcgc
      121 ccgccggccc gaccgccgcc gcaccgtgga ccgcggcggc atcgcgctct gcgtgtggga
      181 gtggggtgac gaggacgcgc cgccgatcct cttcgcccac ggtgggttcg acttcgccgg
      241 tacctacgac acgctcgccc cgctggtggc cgaccagggg tggcgcgtcg tctcctggga
      301 ccagcggggg cacggcgact cgcagcacgg ccacctctac tcctgggacg cggacctgcg
      361 cgacgccgcc gccgtcttgg actcgacgag cgacggcgcc atcccgatcc tcggccactc
      421 caagggcggc gcgctgatgc tccagctcgc cgatgccctg ccgcaccgcg tcagccacgt
      481 cgtcaacctc gacgggctgc cctcggcgcg gtcgtggccc gacgtgcccg accaccaccg
      541 gacgcgcctg ctgaacggcg agctcgccgg atggctcgac caccgccggg tcgccgccga
      601 caagatccgc cgccccggga ccatcgagga gctggccgag cggcgcggtc gcatgaaccc
      661 ccgcatggat cccgactggc tgcgctacct ggttccgatc ggcgcctacg aggaggccga
      721 cgggtggcgc tggaagatcg acgcgtcgat gcgcatgggc ggcttcgggc cgtggcggcc
      781 cgagtggtcg atgtcgcggc tgccgtcgct cggcatgccg gtgctgtgcg tgctcggctt
      841 ggacatcgag gtcatggggt gggggaccct gcccgaagac gtcgtgccca acctgccggc
      901 cggcgcccgg ttcgtgccgc tcgacggcgt cggccacttc gtgcacatcg agaagccggc
      961 cgtcgtggcc gatctcaccc tggagctgat ctcgtgagca ccgtcaccct gctcacccac
     1021 gtgaaggtgc agctggcgct ccacgagctc cgtcccggca ccgggcgccc gctgctgctc
     1081 ctgcacggcc tcggcgagca cacccccgct gcggtcccgg ccagcgcctc ggcctggccc
     1141 ggcccggtct acggcctcga cctcaccggg cacggcgctt cgagccgggc ccccggaggc
     1201 gggtacacgg ccgagatctt gatggccgac gtcgacgccg ccctcgccca cctcgggccc
     1261 gtcaccatcg tcgggcgcgg cctcggtgcc tacgtggcga tgctcatcgc cggcgctcgc
     1321 ccggagctgg tggtgggagc cgtgctgtgc gacggaccgg ggctctccgg tgggggtccg
     1381 gcaccgcact cgccgtcggt cgtcgcgccc accgcagccg cggtcgctgc ggccacaccc
     1441 gagagcgggc tgcccgaccc gttcgccctg gtggagctgg cccgcgacgt gcggcccgag
     1501 gactacgcca ccacctacgc ccgccaggcc ctgcagttct ccgggctcga cgccccgctg
     1561 gccatcgccg cggtcacccg gccgccgtgg ctcgccgcgg tggcggccga gccgggcgtg
     1621 gtggccgaac cggtcgctgc cgcgctcgag cgctacgcgg cgatgccgat gccgacaccg
     1681 acaccgacac cgacgacctg atcgttcggc ccggtcgtcg gcggtgaacg agtgggagct
     1741 gggtcaggcg tcgcgggcga cgacgaacgc tgccaggtcc cgcagcgcca gatcggcgtg
     1801 gggatcgagg cgcagcgccg gcagggcggt gacggcccgc ccggcgaggg cggcgatctc
     1861 ggcctcggcg acgtcgagcg ctccggtcga cacgaacagg gcctggaggt cggcgacctc
     1921 ctgctcgccg aggtccgggt cgccgacgcg tgccagcagg tcgcgctggg cgccgtcggc
     1981 ccgctcgtgc accacggcga gcatcaacgt cggcttgccc tcgcggaggt cgtcgcccac
     2041 gggtttgccg gtgcgctggg tgaggccgaa cacgcccagg acgtcgtctc ggagctggaa
     2101 cgcctcgccc agcgggtcgc cgtactggct caacgcgccg gcgaggccgg ggttcccggc
     2161 gaggacggca ccgacctgca gcggccgttc gatggtgtag cgaccggact tgagccgggc
     2221 gatgcgccgc gctcccgcgc gatcgacacc accggtggcc gtcccggcga gatcgaggta
     2281 ctggccgatg ttgagctcga tccgcagctc gtgccacagc gccgccagct cgggtggacc
     2341 agagggcagc aggaggtccg agtagacgag ggcgaggtcg cccacgagga tggccacgcc
     2401 ctcgccgaag cggcgggact cgccggccca cgcctcggcg gcgtggcggt cggcgaaccg
     2461 gcggtggacg gcgggcgtcc cgcggcgggt ggccgagccg tccatcacgt cgtcgtggat
     2521 cagggcgaag gcctgcagga gctcgaacgc agcgccggca tcgaccacgc gtgcgtcgtc
     2581 gggatcgcct ccggcggcca cgaagcccca atggcagaac acgggccgca gccgcttgcc
     2641 accggccatg accagggccc gcagcgcctc gagcggatgg aggagatcgg cctcgacggc
     2701 ctcccagcgc tcggtctcgt cgtccagcag gcgggtgacc cgcgcctcga cgcgcgccgc
     2761 gaccaggccc aggtcgcctg gcagcgg
//
LOCUS       DATMFS010000014         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_70435, whole genome shotgun sequence.
ACCESSION   DATMFS010000014 DATMFS010000000
VERSION     DATMFS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70435"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNQ33_00260"
     CDS             <1..496
                     /locus_tag="VNQ33_00260"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWJ96554.1"
                     /translation="KTRLQESDLYGNVVDEVRRFLAAKVDEAVAAGVDRESILVDPGP
                     DFTKTPAQTVEVLRHLRELNPEGLPALLAVSRKDFIGALTQTRPQERTAGTLAAVAAV
                     GSGSGVVLRVHDVGEVRNFLTVLDALRSTDPVDPGLRLADELRWAAGRPDGTVATGPV
                     RSAP"
     gene            493..762
                     /locus_tag="VNQ33_00265"
     CDS             493..762
                     /locus_tag="VNQ33_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96555.1"
                     /translation="MTAPQTDPVLVRRARIAKAVTIGQRLGYALFGIATIAFFVGLAA
                     GYTPALVRVIVGCLLGGSAVLAPAIVFGYAVKAAEREEQGLPSGH"
     gene            765..1514
                     /locus_tag="VNQ33_00270"
     CDS             765..1514
                     /locus_tag="VNQ33_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ96556.1"
                     /translation="MTRRLPAVLLALGLLTGAGACGGGSDERPPPKNATTIRVDGTVV
                     NAIMRPPSQSVTGSDELVTVLFLHGQSYTSRIWDDRKILDPVALAGWRAMSVDLPGYG
                     ETPKLPDDGGSTISPGTWLRELVKKLGGPGRVVIVSPSMSGTYSLGYLEQYPEDQLLG
                     FVPVAPVGIDDVERPKDAAPISTMAIWGSEDPSYTQARDAHLLEQMRAPEGAAQSQVI
                     EGASHACYDDEPIEFTSILLSFLRSLDTRSS"
     gene            1624..2730
                     /locus_tag="VNQ33_00275"
     CDS             1624..2730
                     /locus_tag="VNQ33_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWJ96557.1"
                     /translation="MKAPVLTAYDTPLETRDDIEIAPPGPGEVKIRVVASGVCHSDLS
                     VLNGTIPLPTPIVLGHEGAGIVEETGEGVSSVAVGDHVVLSFVPACGECYQCKHQQAY
                     LCEKSAAQGAAGLLDGTTRLTSGGAPLHQMACLGTFGPYAIVPEISTVKIPDDVPLEV
                     AALIGCGVLTGVGAALNTASIVPGDTVAVIGCGGVGLNVIQGAKIAGATTIIAIDMLD
                     SKLEMAEEFGATHTVNASEGDPVAAALAVNGRGADVAFEVIGLGATMEQAINTARPGG
                     EVVLVGVPRMDVFLNLNAAFTFLYLCKTLKGCWYGSSNVYQDVPKLLELWRDGDLKLK
                     ELISQEIDNDHVNEAFEDMKAGTVARSVIVHEHG"
     gene            2727..3344
                     /locus_tag="VNQ33_00280"
     CDS             2727..3344
                     /locus_tag="VNQ33_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ96558.1"
                     /translation="MTNAGPTTSGTQRLPAAERREQLLQVALETFAAKGFHETSMNEV
                     AEAAGVTKPVLYQHFASKKALYNELVDEMGARLERTIFEAVADADGPRQQVEAGFRAY
                     FRWAVAQGPAFRVLFSDRTRADAELAHAVARVESVVADRVASLIDIPGLSGDERHVLA
                     FGVVGIAESTSRHWLKLGLGQGTDADAFADKVARLAWSGLRGITP"
     gene            3390..>4372
                     /locus_tag="VNQ33_00285"
     CDS             3390..>4372
                     /locus_tag="VNQ33_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HWJ96559.1"
                     /translation="MLPADAAPSEDLRDLADRYWEARLEASPLFATFLGDHRYDDRVD
                     DLSAEGEARHRATWLEFLGEAEAVDEAGLDETDRVTRQLLIEEIGDAVSGIDARLIEL
                     TSDQMQGVHADLLTMAGQLRAVEPEHAAMAVTRVRAMGVMLDQAVARYRAGLAAGRTP
                     ARTCIDRSVNQIDGYLASPIEQDPFANLAGPEGWDGEAAWRSDLRAAVEAELRPAFAR
                     YRTALAGELLPAARSDEQPGLCWLPEGDDLYRQLIRLHTGLDLTPEELHEIGMQEATV
                     ELPAQYRALGRRAFGTDDLAEIFAHLLDDADLRYGSGDEIIADARRCLDEA"
BASE COUNT          628 a         1676 c         1485 g          583 t
ORIGIN      
        1 caagacccgg ctccaggaga gcgacctgta cggcaacgtg gtcgacgagg tgcgccggtt
       61 cctggccgcc aaggtcgacg aggcggttgc cgccggggtg gaccgcgagt cgatcctggt
      121 cgacccgggg cccgacttca ccaagacccc ggcccagacg gtggaggtcc tccgccacct
      181 ccgcgagctc aatcccgagg gcctcccggc cctgctggcg gtgtcccgca aggacttcat
      241 cggggccctg acccagacgc gcccccagga gcgcaccgcc ggcacccttg ccgccgtggc
      301 cgccgtgggc agcggttccg gcgtcgtgct gcgcgtccac gacgtcggcg aggtccggaa
      361 cttcctcacc gtgctcgacg cgctgcgcag caccgatccc gtcgatcctg gcctccggct
      421 ggccgacgag ctgcgctggg cggcgggccg gcccgacggg accgtcgcga cgggaccggt
      481 acgatcggcc ccgtgaccgc gccccagacc gaccccgtgc tggtgcggcg ggcccgcatc
      541 gccaaggcgg tcaccatcgg ccagcggctc gggtacgccc tcttcggcat cgccaccatc
      601 gccttcttcg tcggcctcgc cgccggctac acgccagcgc tggtccgggt catcgtcggg
      661 tgcctcctcg gtgggtccgc cgtcctcgct ccggccatcg tgttcgggta cgccgtgaag
      721 gcagccgagc gcgaggagca gggcctcccg tcgggccact gaccgtgacg cgccgcctcc
      781 ccgccgtcct cctcgccctc gggctgctca ccggggccgg ggcgtgcggt ggcggcagcg
      841 acgagcgccc accgcccaag aacgccacca ccatccgggt ggatggcacc gtggtcaacg
      901 cgatcatgcg gccgccctcg cagtcggtga cgggcagcga cgagctcgtc accgtcttgt
      961 tcctgcacgg gcagtcgtac acgtcgcgca tctgggacga ccgcaagatc ctcgatccgg
     1021 tcgcgttggc cggctggcgg gccatgtcgg tggacctccc cggctacggc gagacgccga
     1081 agctccccga cgacggcggc tccaccatct ccccgggcac ctggctgcgg gagctggtga
     1141 agaagctggg cggccccggg cgcgtggtga tcgtgtcgcc ctcgatgtcg gggacctaca
     1201 gcctcggcta cctggagcag tacccggaag atcagctcct cggcttcgtc ccggtggccc
     1261 cggtcggcat cgacgacgtc gagcgcccga aggacgccgc gcccatctcc acgatggcca
     1321 tctggggctc cgaggacccg agctacacgc aggcccgcga cgcccacctc ctcgagcaga
     1381 tgcgggcgcc ggagggggcg gcccagagcc aggtcatcga gggtgcgagc cacgcctgct
     1441 acgacgacga gcccatcgag ttcacctcga tcctgctgtc gttcctgcgc agcctcgaca
     1501 cccgctcgtc ttgagccacc gctcggccga cccggctccg gcggaccgac gccgttcccc
     1561 gcgacttgac cgggcggtca aggtagggtc ggccgcgacc gccgacaccc gaaggggacc
     1621 cccatgaagg cacccgtgct caccgcgtac gacaccccgc tcgagacccg cgacgacatc
     1681 gagatcgcgc caccggggcc gggcgaggtc aagatccggg tggtcgcctc cggcgtgtgc
     1741 cactcggacc tgtcggtcct caacggcacc atcccgctgc ccacccccat cgtcttgggc
     1801 cacgagggcg ccggcatcgt cgaggagacc ggcgagggcg tgagcagcgt cgccgtcggt
     1861 gaccacgtcg tgctgtcgtt cgtccccgcc tgcggcgagt gctaccagtg caagcaccag
     1921 caggcgtacc tgtgcgagaa gtccgcggcc cagggcgccg ccggcctgct cgacggcacc
     1981 acccgtctca ccagcggcgg cgccccgctc caccagatgg cctgcctcgg caccttcggg
     2041 ccctacgcca tcgtccccga gatctccacc gtcaagatcc ccgacgacgt gccgctcgag
     2101 gtcgccgccc tcatcggctg cggcgtgctc accggcgtcg gcgccgccct caacacggcg
     2161 agcatcgtcc cgggtgacac cgtcgccgtc atcggctgcg gcggcgtggg cctcaacgtg
     2221 atccagggcg ccaagatcgc cggggccacc acgatcatcg ccatcgacat gctcgactcg
     2281 aagctggaga tggccgagga gttcggcgcc acccacacgg tcaacgcctc cgagggcgac
     2341 ccggtggccg ccgccctggc ggtcaacggc cgcggcgccg acgtggcgtt cgaggtcatc
     2401 ggcctcggcg ccaccatgga gcaggccatc aacacggccc gaccgggggg cgaggtcgtg
     2461 ctggtgggcg tgccccgcat ggacgtgttc ctcaacctga acgcggcgtt cacgttcctg
     2521 tacctctgca agaccctcaa gggctgctgg tacgggtcgt cgaacgtgta ccaggacgtg
     2581 ccgaagctgc tcgagctgtg gcgcgacggc gacctgaagc tgaaggagct gatctcccag
     2641 gagatcgaca acgaccacgt caacgaggcc ttcgaggaca tgaaggccgg caccgtcgcc
     2701 cgttccgtca tcgtccacga acacggatga ccaacgccgg gccgaccacc tcggggaccc
     2761 agcggctgcc cgcggccgag cgccgcgagc agctgctgca ggtggcgctc gagacgttcg
     2821 cggccaaggg gttccacgag acctcgatga acgaggtcgc cgaggccgca ggggtcacca
     2881 agcccgtcct gtaccagcac ttcgcgtcga agaaggcgct ctacaacgag ctcgtcgacg
     2941 agatgggcgc ccgcctcgag cgcaccatct tcgaggcggt ggccgacgcc gacggcccgc
     3001 gccagcaggt cgaggcgggg ttccgcgcct acttccgctg ggcggtggcc cagggcccgg
     3061 cgttccgggt cctcttcagc gaccgcaccc gtgccgacgc ggagctggcc cacgccgtgg
     3121 cgcgggtgga gtcggtggtc gccgaccgcg tcgcgtcgct catcgacatc cccgggctga
     3181 gcggcgacga gcgccacgtg ctcgccttcg gcgtggtcgg catcgccgag tccacgagcc
     3241 gccactggct caagctcggt ctcggccagg gcaccgacgc cgatgccttc gccgacaagg
     3301 tcgcccgcct cgcctggtcc ggcctccgcg gcatcacgcc ctgacccgcg gcaccgggcc
     3361 cagcgcctcc gtggaccacc atggtgggca tgctccccgc cgacgcagcc ccctctgagg
     3421 acctccgcga cctcgccgac cggtactggg aggcgcggct cgaggccagc cccctgttcg
     3481 ccacgttcct cggcgaccac cgctacgacg accgggtcga cgacctctcc gccgaaggcg
     3541 aagcccgcca ccgggccaca tggctcgagt tcctcggcga agcggaggcc gtcgacgaag
     3601 cggggctcga cgagaccgac cgtgtcacgc gccagctgct gatcgaggag atcggtgacg
     3661 ccgtctcggg catcgatgcc cgcctgatcg agctgacatc ggaccagatg caaggggtcc
     3721 acgccgacct cctgacgatg gccgggcagc tccgcgccgt cgagcccgag cacgcggcga
     3781 tggcggtcac ccgcgtgcgg gcgatgggcg tgatgctcga ccaggccgtc gcccgctacc
     3841 gcgccggcct ggcggccgga cgcacaccgg cacggacctg catcgaccgg tcggtcaacc
     3901 agatcgacgg gtacctggcc tcgcccatcg agcaggaccc cttcgccaac ctggccggcc
     3961 ccgagggctg ggacggcgag gcggcctggc ggtccgacct gagggctgcc gtcgaggccg
     4021 agctgcgccc ggcgttcgcc cgctaccgca cggccctggc cggtgagctg ctgccggccg
     4081 cccgctccga cgagcagccc ggcctgtgct ggctccccga gggcgacgac ctctaccgcc
     4141 agctcatccg cctccacacc ggcttggacc tcacacccga ggagctgcac gagatcggga
     4201 tgcaggaggc cacggtggag ctgccggcgc agtaccgcgc cctcggacgg cgggcgttcg
     4261 gcaccgacga cctggccgag atcttcgccc acctgctcga cgacgcggac ctgcgctacg
     4321 ggagcggcga cgagatcatc gccgatgccc gccgctgcct cgacgaggcc ag
//
LOCUS       DATMFS010000015         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_70515, whole genome shotgun sequence.
ACCESSION   DATMFS010000015 DATMFS010000000
VERSION     DATMFS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70515"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VNQ33_00290"
     CDS             <1..1200
                     /locus_tag="VNQ33_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ96560.1"
                     /translation="CIMCGADRSSTIRARRLGLPPLPDRYPDQFFEEIGEFIEHASIC
                     DFVGGEPLLVRGHWRIWDIMREVAPGIDCAITTNGTIWNPRVEALLHDFPTSVRLSMD
                     GVTRETFEAVRVGADYDRFMANFDRFYAYSRASGRELTLSWSFIQQNWWELADAMVWA
                     EEHGVVVYVTTVLDRGFGVQHLPDDELRVVHERMLQQSDAIRPSLQLNRENWDRQVTM
                     VSAELEARTTGIRIDPSVEPPTPDAPQRTEQAILAQVAHLDPLPGSPDLRTFLAGWEL
                     DLRGADVLRCDPSGLVVAHQRGEPSIVRGIQLGTDQIADVLARLCDQLDGHLWRMEEI
                     HLVDAFVATVAIGAEVRDGDPVVMVRLVATRVGDQIELCLSVDGRSAQATDVPVVITA
                     VAASSGG"
     gene            complement(1209..1847)
                     /locus_tag="VNQ33_00295"
     CDS             complement(1209..1847)
                     /locus_tag="VNQ33_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWJ96561.1"
                     /translation="MSTTAPGVSTDMAITPEEIASKEFLVGLRGYDKDEVRAYLQTVA
                     AAFDAIPAAAAAPAAAPAPSAEGGLTAQLGQIEAILATANAEAAKVRSEAEADAARTR
                     AEADAHAQATRAEAESYAEATRAEAEQHANEARQKLTAAQDEALGLVADAQARVDKMV
                     ESSRVEAEAAAQASVAHLTTQVDELTAARDASKAQLTELRTKLDKALTIAEG"
     gene            1983..3329
                     /gene="ccrA"
                     /locus_tag="VNQ33_00300"
     CDS             1983..3329
                     /gene="ccrA"
                     /locus_tag="VNQ33_00300"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWJ96562.1"
                     /translation="MQAFLDAILAGASGDELANIDIPESYRAAFVKRDEQTMWEGYES
                     EDKDPRKSLHVDQVATPELAPDEVYVAVMASAINFNTVWTSIFEPLPTFGFLDRLGKE
                     SVWGARHALPYHVVGSDASGVVVRVGSAVRNWKPGDKVTLHCNHLDDQDPSAHDDSML
                     ASNQRIWGFETNFGGLADLTVAKANQLMPKPKHLTWEEAACNALCNSTSYRMIVSPHA
                     GNLTQGDAVLVWGATGGIGGYAVQYILNGGGTPVGVVSSDKRRRLLNEMGVEHVIDRR
                     EADYKFWKDEHTQDEKEWRRLGKDIRGLIGRDVDTVFEHPGRQTFGASVFAAARGGKI
                     VTCAATSGYMIEYDNRHLWMKLKSIVSSHFANYKEAWAANQLIAEGKIQPLLSRTYAL
                     EETGEAALAVHKNQIEGKAGVLCLAPEKGLGIDDPEFREKVGEDKITLFQRFDQQD"
     gene            3473..3976
                     /locus_tag="VNQ33_00305"
     CDS             3473..3976
                     /locus_tag="VNQ33_00305"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ96563.1"
                     /translation="MGIQAKNPNEDDDQFETSFSGEPVDEAFDGGLDAAVLTDVDHIA
                     IVVEDLDEAIAEHRDTYGVLVEHREEMHGEGYEVAFLVVGGSTIQLISPTSEDSEFAE
                     FLAEGGPGLHHIGYRVDDVAAAVTALSSNDRDVLDEEPVAGPRESKTAFVHPDSSFGV
                     LVQLVER"
BASE COUNT          638 a         1405 c         1434 g          622 t
ORIGIN      
        1 tgcatcatgt gcggcgcgga tcgttcgtcg acgatccggg cgcggcgctt gggcttgcca
       61 ccgctgccgg accggtaccc cgaccagttc ttcgaggaga tcggtgagtt catcgagcac
      121 gcctcgatct gcgacttcgt cgggggcgag ccgctcctgg tcagggggca ttggcgcatc
      181 tgggacatca tgcgcgaggt ggcaccgggc atcgactgcg cgatcaccac caacggcacg
      241 atctggaacc cgcgggtcga ggccctgctg cacgacttcc ccaccagcgt gcgcctctcg
      301 atggacgggg tgacgcgcga gaccttcgag gccgtgcggg tcggggctga ctacgaccgg
      361 ttcatggcga acttcgaccg cttctacgcc tactcccgtg cgagcgggcg ggagctcacg
      421 ctgtcgtggt cgttcatcca gcagaactgg tgggagctgg ccgacgccat ggtctgggcc
      481 gaggagcacg gcgtggtcgt gtacgtgacc acggtgctcg atcgaggttt cggcgtgcag
      541 cacctccccg atgacgagct tcgggtcgtc cacgaacgga tgctccagca atccgacgcg
      601 atccggccgt cgctccagct caaccgcgag aactgggacc ggcaggtgac gatggtgagc
      661 gcggagctgg aggcccggac caccgggatc cgcatcgatc catcggtcga accgccgacg
      721 cccgacgcgc cgcagcgcac cgagcaggcc atcctcgccc aggtcgcgca cctggacccg
      781 ctccctgggt ccccggatct gcgcaccttc cttgccgggt gggagctcga tctccgcggc
      841 gccgacgtcc tccgctgcga tccgtccggc ctcgtggtcg cgcaccagcg cggcgagccg
      901 tcgatcgtgc gtgggatcca gctcgggacc gaccagatag cggacgtgct ggctcggctg
      961 tgcgaccagc tcgatggtca cctgtggcgc atggaggaga tccacctcgt cgacgcgttc
     1021 gtggccaccg tcgcgatcgg cgcagaggtg cgtgacgggg acccggtcgt gatggtccgg
     1081 ttggtcgcca cccgagtggg cgatcagatc gagctgtgcc tgagcgtcga cgggcgaagc
     1141 gcacaggcca ccgacgtgcc ggtggtgatc accgccgtcg ctgcatcgag cggcggctga
     1201 agctcaggtc agccctcggc gatggtgagg gccttgtcga gcttggtccg cagctcggtg
     1261 agctgcgcct tggatgcatc gcgggcggcg gtgagctcat cgacctgcgt ggtgaggtgg
     1321 gcgaccgatg cctgggcggc ggcttcggcc tccacccggg agctctcgac catcttgtcg
     1381 acgcgggcct gggcgtcggc gaccaggccg agggcctcgt cttgggccgc ggtcagcttc
     1441 tggcgagcct cgttggcgtg ctgctccgcc tcggcgcggg tggcctccgc gtagctctcc
     1501 gcctcagcgc gggtggcctg ggcgtgggcg tcggcctcgg cgcgggtgcg cgccgcgtcg
     1561 gcctcagcct ccgaacggac cttggcggcc tcggcgttgg cggtggcgag gatggcctcg
     1621 atctggccga gctgggccgt gagaccgccc tcggccgagg gtgccggagc cgcagcaggg
     1681 gccgcggccg cagccgggat ggcatcgaac gcggcggcga cggtctgcag gtaggcgcgg
     1741 acctcgtcct tgtcgtaccc gcgaaggccg acgaggaact ccttgctggc gatctcctcg
     1801 ggtgtgatgg ccatgtccgt gctgactcct ggtgcggtgg tgctcacgag gacactatcg
     1861 gcggcgatct ccgcgacgag aggtatgggc ggcgcagcat gggtcacacg gcccacgccg
     1921 gtcgtgcgac gtgccgccgg cacttcgtct gctgacggat gtcccacata ggatgcccgg
     1981 ccatgcaggc attccttgac gccatcttgg ccggtgcttc cggcgacgag ctcgccaaca
     2041 tcgacatccc ggagtcgtac cgggcagcgt tcgtgaagcg cgacgagcag accatgtggg
     2101 aggggtacga gtccgaggac aaggaccccc gcaagtccct ccacgtcgac caggtcgcca
     2161 cgccggagct ggcgcccgac gaggtgtacg tcgcggtgat ggcgtcggcc atcaacttca
     2221 acacggtctg gacctcgatc ttcgagccgc tgccgacctt cggcttcctc gaccgcctcg
     2281 gcaaggagag cgtgtgggga gcccgccacg ccctgccgta ccacgtggtc gggtccgatg
     2341 cttcgggcgt cgtcgtccgc gtcggttcgg cggtgcgcaa ctggaagccg ggcgacaagg
     2401 tcacgttgca ctgcaaccac ctcgacgacc aggacccgtc cgcccacgac gactccatgc
     2461 tcgccagcaa ccagcgcatc tggggtttcg agaccaactt cggcggcctg gcggacctca
     2521 ccgtcgccaa ggccaaccag ctcatgccga agcccaagca cctcacgtgg gaagaggcgg
     2581 cgtgcaacgc gctgtgcaac tccacgagct accgcatgat cgtcagcccc cacgccggca
     2641 acctgaccca gggcgacgcc gtgctggtct ggggcgcgac cggcggcatc ggcggctacg
     2701 ccgtgcagta catcctcaac ggcggcggca cgccggtggg cgtggtctcc tcggacaagc
     2761 gccgccgcct cctcaacgag atgggcgtcg agcacgtcat cgaccgccgc gaggccgact
     2821 acaagttctg gaaggacgag cacacccaag acgagaagga gtggcgccgc ctcggcaagg
     2881 acatccgcgg cctcatcggg cgcgatgtcg acaccgtgtt cgagcacccc ggccgccaga
     2941 cgttcggcgc ctcggtcttc gccgcggcac gcggcggcaa gatcgtcacc tgcgctgcca
     3001 cctccggcta catgatcgag tacgacaacc gccacctctg gatgaagctg aagagcatcg
     3061 tctcctcgca cttcgccaac tacaaggagg cgtgggccgc caaccagctc atcgccgagg
     3121 gcaagatcca gccgctgctc agccgcacct acgcgctcga agagaccggc gaggccgccc
     3181 tggccgtcca caagaaccag atcgagggca aggccggcgt gctgtgcctg gcacccgaga
     3241 agggcctcgg catcgacgac cccgagttcc gcgagaaggt cggcgaggac aagatcacgc
     3301 tgttccagcg cttcgaccag caggactgaa tcgttccagc ggttcgacca gcggggctga
     3361 gcctcccgaa cccaagaccg gcgcgatctg gcgtagacca gaggccctga cggctccggt
     3421 ctgggcccga tccgcgtctg gaccgaagcg acgaagacga agcaggagca cgatgggcat
     3481 ccaggccaag aacccgaacg aggacgacga ccagttcgag acgtcgttca gcggcgagcc
     3541 ggtcgacgag gcgttcgacg gcgggctcga cgcggccgtg ctcaccgacg tcgaccacat
     3601 cgcgatcgtg gtggaggacc tcgacgaggc catcgccgag caccgcgaca cctacggggt
     3661 gctggtcgag caccgcgagg agatgcacgg cgagggctac gaggtggcgt tcctcgtcgt
     3721 cggcgggtcg acgatccagc tcatctcgcc caccagcgag gactccgagt tcgccgagtt
     3781 cctggccgag ggcgggccgg ggctgcacca catcggctac cgcgtcgacg atgtcgccgc
     3841 tgctgtcacc gccttgtcga gcaacgaccg cgacgtgctc gacgaggagc cagtggccgg
     3901 tcctcgcgaa tcgaagacgg ccttcgtgca ccccgacagc tcgttcggcg tcctcgtcca
     3961 gctcgtcgag cggtagccgc cccgccggcc cgggctggtc gatcatcgac cttggccggg
     4021 gaggcgcggc cgatgtggca cctggtgggt agccggcccc gccgggccgt cggtgcggcg
     4081 tgggcgaccg accaggctg
//
LOCUS       DATMFS010000016         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_93434, whole genome shotgun sequence.
ACCESSION   DATMFS010000016 DATMFS010000000
VERSION     DATMFS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93434"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1579
                     /locus_tag="VNQ33_00310"
     CDS             407..1579
                     /locus_tag="VNQ33_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWJ96564.1"
                     /translation="MASTEASTKFVNLDIHLWEWAALLAFITILLMVDLLLVHRRPHE
                     IHFKEAAIESAIWISIGVAFSAVIWYIGSTSGVGPDGAHGIDVGAKAAGEYISGFLLE
                     KSLSVDNVFVWAVIFTYFAVPKMYQFRVLFWGIFGALVLRAIFIFAGSALISKFTVTL
                     AVFGVILLYSAYKLAFQEETEVDPEKSIVLRAVRKVVPSTDQLDGQKLFTLQNGRRLA
                     TPLFAVLVMIETTDVIFAVDSVPAIFGVAREPFIVFSSNAFAILGLRALYFLLGGMQD
                     RFRYLHYGLGAILAFVGLKMLLTFSAEQFEFPSAGFHFPTAASLGIILGILVITIVAS
                     MLADKRDPRLGLDSHMPGHATLYERKQRGDDLDGDGEPDPVSEVGVLGQDDADPNT"
     gene            1703..>2569
                     /gene="dnaG"
                     /locus_tag="VNQ33_00315"
     CDS             1703..>2569
                     /gene="dnaG"
                     /locus_tag="VNQ33_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWJ96565.1"
                     /translation="MGILDEDVQRVRETADIVAVISAHTQLKKQGANWSGLCPFHGEK
                     SPSFSVNADKGVFYCFGCQAKGDVISFVRDIDHLDFAGAVEFLAAKSGITLRYTDRAE
                     GDRREARGKLREAMAAAVEWYHQRLLTGSDSGAARRYLRDRGFDGDRVRQYKIGWAPD
                     DWDQLVKALPFSPKVLRDAGLGRESTRGGTNDFFRARVLFPIFDVQGDPIGFGGRMMP
                     GGRPPKYQNTPETSLYRKSKVLYGLNWAKDEAVRENEVIICEGYTDVIGFAEVGLHRA
                     VATCGTALTDEHV"
BASE COUNT          388 a          900 c          847 g          434 t
ORIGIN      
        1 cccggagggc ctggacctgc tcggtggtga gctccgaggc ggcgatgatc ggtccgaggt
       61 cgagcgcttc ggccttctcg gcgaggtccg cggaccgctt gtccagctcg atcgccagtt
      121 cgtacaggtg cacgggggtc ctccaccctc gggtcctggt caccatcggc gcacccgagg
      181 gcgatcttga gccgagaggc ggaggcgcgg cctcccgatg cagctccccg ggtgcccggc
      241 gccgtagggt ggacggaccg ggagggagta tccctcgtgg ccatcacgtc agcacggtgc
      301 cctcacggca cccggggtgg ccggttccct gtgggagcgg agagacttcc ggctgtagga
      361 ccctgatcca ccgaacagcc ggagcaaccc tttgtcaccc ctcctgttgg ccagcaccga
      421 agccagcacc aagttcgtca acctcgacat ccacctgtgg gagtgggctg cgctcctcgc
      481 gttcatcacg atcctgctga tggtcgacct cctcttggtc caccggcgtc cgcacgagat
      541 ccacttcaag gaggcggcca tcgagtccgc catctggatc tcgatcggtg tcgcgttcag
      601 cgccgtcatc tggtacatcg ggtcgacgtc aggcgtcggc cccgacggtg cccacggcat
      661 cgacgtcggc gccaaggccg ccggggagta catctccggc ttcctgctcg agaagagcct
      721 gtcggtggac aacgtcttcg tctgggccgt gatcttcacg tacttcgcgg tgccgaagat
      781 gtaccagttc cgggtgctct tctggggcat cttcggggcg ctcgtcctgc gtgccatctt
      841 catcttcgcc gggtcggccc tgatctcgaa gttcaccgtc acgctcgccg tcttcggcgt
      901 catcctcctg tactcggcct acaagctggc gttccaggag gagaccgagg tcgaccccga
      961 gaagtcgatc gtgctgcgag cggtgcgcaa ggtggtcccg tccaccgacc agctcgacgg
     1021 ccagaagctc ttcaccctcc agaacggccg gcgcctcgcc acgccgctct tcgccgtgct
     1081 ggtcatgatc gagaccaccg acgtcatctt cgccgtcgac tccgtgcccg ccatcttcgg
     1141 cgtcgcccga gagccgttca tcgtcttcag ctcgaacgcc ttcgccatcc tcggcctgcg
     1201 ggcgctgtac ttcctgctcg gcgggatgca ggaccggttc cgctacctgc actacggcct
     1261 cggcgcgatc ctcgcgttcg tcgggctcaa gatgctgctc accttctcag ccgagcagtt
     1321 cgagttccct tcggccgggt tccacttccc gaccgcggcc tcgctcggca tcatcctcgg
     1381 gatcttggtg atcacgatcg tggcgtcgat gctcgcggac aagcgcgacc cgcgcctggg
     1441 cctcgacagc cacatgccgg gccacgccac gctgtacgag cgcaagcagc gcggtgacga
     1501 cctcgacggc gacggcgagc ccgacccggt gtccgaggtc ggcgtgctcg gccaagacga
     1561 cgccgatccc aacacctgac gaccgggccg caaggcccgg gtcgatggat ccgaaggacg
     1621 gtgccagcag cgccgctggt accgtccgcc ggatgatcag cgccgtcgag acccacccgg
     1681 ctgtgtgccg gcgagcggtc tgatgggcat cctcgacgag gacgtgcagc gggtccgcga
     1741 gaccgccgac atcgtggcgg tcatctcggc gcacacgcag ctgaagaagc agggcgccaa
     1801 ctggagcggt ctgtgcccgt tccacggcga gaagagcccg tcgttctcgg tcaacgccga
     1861 caagggcgtc ttctactgct tcggctgcca ggcgaagggc gacgtcatct cgttcgtccg
     1921 cgacatcgac cacctggact tcgccggtgc cgtggagttc ctggccgcca agtccggcat
     1981 cacgctgcgc tacaccgacc gggccgaggg cgaccgtcgc gaggcgcggg gcaagctccg
     2041 cgaggcgatg gccgcggcgg tcgagtggta ccaccagcgg ctgctcaccg gttcggactc
     2101 gggtgcggct cgccgctacc tccgcgaccg cgggttcgac ggcgaccggg tgcgccagta
     2161 caagatcggc tgggcgcccg acgactggga ccagttggtg aaggcactgc cgttctcgcc
     2221 caaggtcctg cgtgacgcag gactcggacg cgagagcacg cgcggcggta ccaacgactt
     2281 cttccgggcg cgggtgctgt tcccgatctt cgacgtgcag ggcgacccca tcgggttcgg
     2341 cgggcgcatg atgcccgggg ggcggccacc caagtaccag aacaccccgg agacctccct
     2401 ctaccgcaag agcaaggtcc tgtacgggct gaactgggcc aaagacgagg cggtccggga
     2461 gaacgaggtc atcatctgcg agggctacac cgacgtgatc ggcttcgccg aggtcggcct
     2521 gcaccgggcc gtcgccacgt gcggcaccgc gctgaccgac gagcacgtg
//
LOCUS       DATMFS010000017         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_210082, whole genome shotgun sequence.
ACCESSION   DATMFS010000017 DATMFS010000000
VERSION     DATMFS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210082"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VNQ33_00320"
     CDS             <1..368
                     /locus_tag="VNQ33_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWJ96566.1"
                     /translation="EDAQRKEEADIRNQADTLVYQTDKLLSEQGEKVTGPEKDALEAA
                     LATLKTANEGQDLEAMKSAIEEVSTTSQAFAQKLYETTATENAGQYDAGAASGAGATG
                     ADDDDVVDAEVIDEDGDDK"
     gene            365..922
                     /locus_tag="VNQ33_00325"
     CDS             365..922
                     /locus_tag="VNQ33_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWJ96567.1"
                     /translation="MSDAQDPGVAEGTAPPDPEVDVEAILEDELVGDAVDGATSVEDL
                     VALLEETTAQRDEYLDLARRTQAEFENHRKRVAKQLDDEVVRKVAELVEQLLPVLDAC
                     DGAVAHGATEVEPIFAALLQTLEKRGLERIDPAGREFDPNAHEAVMHEPADEGQETTL
                     VSDVMRAGYAWQGRVVRPAMVKVKG"
     gene            939..>2034
                     /gene="dnaJ"
                     /locus_tag="VNQ33_00330"
     CDS             939..>2034
                     /gene="dnaJ"
                     /locus_tag="VNQ33_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWJ96568.1"
                     /translation="MNNPPREWFEKDYYEVLGVDEKADAKEVTRAYRKLARELHPDAN
                     PGDAAAEERFKEVSSAYDVVGDADKRKDYDEVRALGPMGGGMGGFGGGPGAGGFGGPG
                     GPGGFTFDMGGAGGGLGDVLGNLFGGGGGRGGGRSRATGPQRGSDLEAELHLDFADAV
                     AGLSTSLHLVSDAVCHTCAGSGAEPGTTPTRCPVCAGRGSVEDNQGMFAMSRPCPNCA
                     GRGTLIEHPCPTCRGNGVERRPREIKVRIPAGVSDGQRIRLKGRGDPGRQGGQPGDLF
                     VTCRVEPHPVFSMEARNLRVTVPITFAEAALGADIAVPTIDGGSVKVRIPAGTRTGKT
                     FRVKGRGVPAAKGTGDLMVGVEVAVPAKLSD"
BASE COUNT          323 a          746 c          736 g          229 t
ORIGIN      
        1 ccgaggacgc ccagcgcaag gaggaggccg acatccgcaa ccaggccgac accctcgtgt
       61 accagaccga caagctgctc tccgagcagg gcgagaaggt caccgggccc gagaaggacg
      121 cgctggaggc ggccctcgcc accctcaaga ccgcgaacga gggccaggac ctcgaggcca
      181 tgaagtcggc catcgaagag gtgtccacca cctcgcaggc cttcgcccag aagctctacg
      241 agaccaccgc cacggagaac gccggccagt acgacgccgg tgccgcgagc ggtgccgggg
      301 ccaccggtgc cgatgacgac gacgtcgtcg acgccgaggt catcgacgag gacggcgacg
      361 acaagtgagc gacgcccagg accccggggt cgcggagggc accgcccctc ccgacccgga
      421 ggtcgacgtc gaggcgatcc tggaggacga gctcgtcggc gacgccgtcg acggcgccac
      481 ctccgtggag gatctcgtcg ccctcctcga agagaccacc gcccagcgcg acgagtacct
      541 cgacctcgcc cggcggaccc aggccgagtt cgagaaccac cgcaagcgcg tggccaagca
      601 gctcgacgac gaggtcgtgc gcaaggtcgc cgagctcgtg gagcagctgc tccccgtgct
      661 cgacgcgtgc gatggtgccg tcgcccacgg cgccaccgag gtcgagccga tcttcgccgc
      721 cctgctccag acgctggaga agcgcggcct cgagcggatc gatccggccg gccgcgagtt
      781 cgacccgaac gcgcacgagg ccgtgatgca cgagcctgcc gacgaaggcc aggagaccac
      841 cctcgtgtcc gacgtcatgc gcgccggcta cgcctggcag ggccgcgtcg tccgccccgc
      901 catggtcaag gtgaagggct gaggggccgg agggaagcgt gaacaacccg ccgcgggaat
      961 ggttcgagaa ggactactac gaggtcctgg gcgtcgacga gaaggccgac gccaaggagg
     1021 tcacccgcgc ctatcggaag ctggcacggg aactgcatcc cgacgccaac cccggtgacg
     1081 ccgcggccga ggagcgcttc aaggaggtgt cctcggcgta cgacgtcgtc ggcgacgccg
     1141 acaagcgcaa ggactacgac gaggtccgag ccctcggccc gatgggcggg ggcatgggcg
     1201 gcttcggcgg tggacccggc gccggcggct tcggcggccc cggcggccct ggcgggttca
     1261 ccttcgacat gggcggcgcc ggtggcggcc tgggcgacgt cctgggcaac ctcttcggcg
     1321 ggggcggcgg tcgaggcggc ggccggtcgc gggccacagg tccccagcgg ggctcggacc
     1381 tcgaggccga gctccacctc gacttcgccg acgccgtggc cggcctgtcc acctccctgc
     1441 acctcgtcag cgacgcggtg tgccacacct gcgccggatc gggcgccgag cccggcacga
     1501 cgcccacccg ctgcccggtc tgcgccggcc gggggagcgt cgaggacaac cagggcatgt
     1561 tcgccatgtc ccgcccctgc ccgaactgcg ccgggcgcgg cacgctcatc gagcacccgt
     1621 gccccacgtg ccggggcaac ggggtcgagc gccggccgcg cgagatcaag gtccgcatcc
     1681 ccgccggtgt gagcgacggc cagcgcatcc ggctcaaggg ccggggcgat ccggggcgcc
     1741 agggcggcca gcccggcgac ctcttcgtca cctgccgcgt cgagccccac ccggtcttct
     1801 cgatggaggc ccggaacctg cgggtcaccg tgcccatcac gttcgccgag gctgcgctcg
     1861 gtgccgacat cgccgtgccc accatcgacg gcggctcggt caaggtccgc atccccgcgg
     1921 gcacccggac ggggaagacg ttccgcgtga agggccgggg cgtccctgcc gccaagggca
     1981 cgggggacct gatggtcggc gtcgaggtgg ccgttccggc caagctgtcg gatg
//
LOCUS       DATMFS010000018         3902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_163411, whole genome shotgun sequence.
ACCESSION   DATMFS010000018 DATMFS010000000
VERSION     DATMFS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163411"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VNQ33_00335"
     CDS             <1..467
                     /locus_tag="VNQ33_00335"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ96569.1"
                     /translation="RNGGFSTADFAQLYLPALMDPVYGYQAVNVESQLRNPGSFLHWV
                     QRMIQARKQHPVFGTGSFEALEVANEAVLVFLRRQARDDSADDVVLCVNNLSKVAQPV
                     EIDLSMLAGKQPVELFGRVEFPPVGELPYFVTLPPYGFYWFSLVDETDDDLE"
     gene            514..1407
                     /locus_tag="VNQ33_00340"
     CDS             514..1407
                     /locus_tag="VNQ33_00340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWJ96570.1"
                     /translation="METGYQTCYRHADRQAGVICQRCDRPICPECMHQASVGFHCPEC
                     TKKGAQKVYQGVPSLRERPIVTEVLMGLNVLVFVIGAVAVASGNGVADYFGGASTSFH
                     VDWASTTYVYVNRLTGELSGGVGGGEWYRLVTSGFVHFGIIHLAVNMYSLWILGRAAE
                     QYAGRLKFGVIYGVSLLAGSFGSLVLEPTALSAGASGAIFGLMGAMFLAFRAEGIPLS
                     RSPLLSVLILNLIITFSVPNIAIGAHLGGLVGGAITGWAFFDLARRPGLDQRIPYAVS
                     GALAVALVVGSIVFASGYAPS"
     gene            complement(1423..1869)
                     /locus_tag="VNQ33_00345"
     CDS             complement(1423..1869)
                     /locus_tag="VNQ33_00345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ96571.1"
                     /translation="MGRIRVSTIIDAPPALVWDEVRHIDRHVDWMADAAEIRFTSRRR
                     SGVGTTFDCKTKVGPIRLTDKMEITDWRDGKVMGVRHIGLVTGTGRFTVTKARGGRTR
                     FTWDERLRFPWWLGGPVGGVVGGRAMRQIWKRNLRVLKALVESGRR"
     gene            2079..3194
                     /locus_tag="VNQ33_00350"
     CDS             2079..3194
                     /locus_tag="VNQ33_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ96572.1"
                     /translation="MDSWTLALVASAVALVGVAAVVAVAVRANGRLTLRSLAVPAAVL
                     ALASVAASLPVEGLARSSRTFAVVHLAYLLFGIAVPAVAVAVLVAAAVRGATWPVWAV
                     SAVLLLPAPVSWYATHIVPFRLGVERAEVQLPAERSGSDPIRVGVLADLQTTRITSYE
                     EEAVTKLLSLQPDVILLPGDLFQGSDAQFRAQLPAFRRLLGRLDAPGGVYAVRGDTDQ
                     GDKLDLLVEGTSIEILDYRVVDVEIGDRRVRIGGNALLWANPPAVKSRQELLAGSAGT
                     VRILLSHRPDVALLLPRDSGVDLTVAGHTHGGQVALPLLGPVMTFSSVSRSVARGGRH
                     DVNGNELFVSSGVGMARLDAPQVRLGTRPAVGLVTLR"
     gene            complement(3311..>3902)
                     /locus_tag="VNQ33_00355"
     CDS             complement(3311..>3902)
                     /locus_tag="VNQ33_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWJ96573.1"
                     /translation="AALRDRLEDHAATAGIRYSQAIVNHGLAAGASRRHPHGQLLGIP
                     FVPGELIEELAGFRRFQGGCLLCATLAAERDAGYRTIAIDDDVAVIAPWWSGSPFELL
                     VIPVQHEGHLHHAKPDSLVAVGTAIRDALARLRTLLGDVSYNIVFHTLPHRAGDPFHW
                     HAHITPRVHSTGGFEQGTGVPINIVAPEDVAAFLAG"
BASE COUNT          469 a         1357 c         1442 g          634 t
ORIGIN      
        1 accgcaacgg cgggttctcc accgccgact tcgcccagct ctacctgccg gcgctcatgg
       61 acccggtgta cggctaccag gcggtcaacg tcgagtcgca gctgcggaac cccgggtcct
      121 tcctccactg ggtgcagcgc atgatccagg cccgcaagca gcacccggtg ttcggcaccg
      181 gttcgttcga ggcgctcgag gtggccaacg aggccgtgct ggtgttcctg cgcaggcagg
      241 cgcgagacga cagcgccgac gacgtcgtgc tgtgcgtcaa caacctgtcg aaggtggccc
      301 agccggtcga gatcgacctg tcgatgctgg cgggcaagca gcccgtcgag ctgttcggcc
      361 gggtggagtt cccgccggtc ggcgagctgc cctacttcgt caccctgccg ccgtacggct
      421 tctactggtt ctcgctcgtc gacgagaccg atgacgacct tgagtagtcc tgccgacccg
      481 ggcaggtcgg tgcgccccgc taccgtcgca ccggtggaga ccggctacca gacctgctac
      541 cgccacgccg accgtcaggc cggtgtgatc tgccaacggt gcgaccgacc gatctgcccc
      601 gagtgcatgc accaggcgtc ggtcggcttc cactgcccgg agtgcaccaa gaagggcgcc
      661 cagaaggtgt accagggcgt tccgtcgctc cgcgagcgcc cgatcgtcac cgaggtcctg
      721 atgggcctca acgtgctggt cttcgtgatc ggggcggtgg cggtcgcatc ggggaacggc
      781 gtcgccgact acttcggcgg ggccagcacg tcgttccacg tcgactgggc atccaccacg
      841 tacgtctacg tcaaccggct caccggcgag ctctcgggcg gggtgggcgg cggggagtgg
      901 taccggttgg tcacgtccgg gttcgtccac ttcgggatca tccacctggc cgtgaacatg
      961 tactcgctgt ggatcctcgg ccgtgccgcg gagcagtacg ccggccggct caagttcggg
     1021 gtcatctacg gggtctcgct gctcgccggc tcgttcggtt cgctcgtctt ggagccgacc
     1081 gcgctctcgg ccggtgcgtc gggcgccatc ttcggtctga tgggggcgat gttcctcgcc
     1141 ttccgggccg agggcatccc cctgtcccgc tcgccgctgc tgagcgtgct gatcctcaac
     1201 ctgatcatca cgttctcggt gccgaacatc gcgatcggcg cccacctggg cggcctcgtc
     1261 ggtggcgcga tcaccggctg ggcgttcttc gacctcgccc gtcggccggg gctggaccag
     1321 cggatcccct acgcggtgtc cggggcgctc gccgtggcgc tggtggtcgg atcgatcgtg
     1381 ttcgcttccg gctacgcgcc ctcctgacgc ggcggggtgc gatcagcggc ggccgctctc
     1441 gaccagggcc ttgaggacgc gcaggttgcg cttccagatc tggcgcatgg cgcgcccgcc
     1501 cacgacaccg cccacggggc cgccgagcca ccacgggaac cgcaggcgct cgtcccacgt
     1561 gaaccgggtg cgcccgcccc gggccttggt gacggtgaag cggccggtcc cggtcacgag
     1621 gccgatgtgg cggacgccca tgaccttgcc gtcgcgccag tcggtgatct ccatcttgtc
     1681 ggtcaggcgg atggggccca ccttggtctt gcagtcgaac gtcgtgccca cgccggaccg
     1741 ccggcgcgat gtgaagcgga tctcggcggc gtcggccatc cagtccacgt ggcggtcgat
     1801 gtgacgcacc tcgtcccaca ccagcgcggg tggtgcatcg atgatggtcg agacccggat
     1861 gcggcccatg ccctgctccc atgtcaagcg gcagccgcga ggctaggagg aggcgcccgc
     1921 gctgctcgac tccgtagatc cgcttcccgg cccgacctcg gtccgacacg atcgaggtga
     1981 ccgccccggt ccggtgatcg tggtgctcgg ccccaccggg caggcggccg tcgggggtgc
     2041 gggtgctctg gccggggcgg tctggaaggc tggcggcgat ggactcctgg acgctggcgc
     2101 tcgtcgcatc ggccgtcgcg ctcgtggggg tcgcggcggt ggtggccgtc gccgtgcggg
     2161 cgaacggccg cttgacgctg cggtcgctgg cggtcccggc ggcggtgctc gctctggcgt
     2221 cggtcgccgc gtcgctcccc gtcgagggtc tggcgcggtc gtcgaggacc ttcgccgtgg
     2281 tccacctggc gtacctcctg ttcggcatcg ccgtgcctgc cgtggccgtc gccgtcttgg
     2341 tcgcggcggc ggtgcgaggc gccacctggc cggtctgggc ggtgtcggcg gtgctgctcc
     2401 tgccggcgcc ggtgtcttgg tacgcgacgc acatcgtccc gttccgcctc ggtgtggagc
     2461 gggccgaggt gcagctgccg gcggagcggt cgggctcgga tcccatccgc gtcggcgtgc
     2521 tggcggacct gcagaccacc cgcatcacct cctacgagga ggaggcggtg acgaagctgc
     2581 tgtcgctcca gcccgacgtg atcctcctgc ccggcgacct gttccagggg agcgatgccc
     2641 agttccgcgc ccagctgccg gccttccgcc ggctcctcgg ccgcctcgac gcgccgggag
     2701 gcgtctacgc cgtgcggggc gacacggacc agggcgacaa gctcgacctg ctggtcgagg
     2761 ggaccagcat cgagatcctc gactaccgcg tggtggacgt cgagatcggt gaccgccggg
     2821 tgcgcatcgg cggcaacgcc ctgctgtggg cgaacccgcc ggcggtgaag agccgccagg
     2881 agctgctggc gggcagcgct gggacggtcc ggatcctgct ctcgcaccgg cctgacgtgg
     2941 cgctgctgct cccacgggac tcgggtgtcg acttgaccgt ggccggtcac acgcacggcg
     3001 gtcaggtcgc gctgccgctg ctcggcccgg tgatgacgtt ctcgtccgtc tcccggtcag
     3061 tcgcccgagg gggccggcac gacgtgaacg gcaacgagct cttcgtcagc tccggcgtcg
     3121 gcatggcccg gctcgacgcg ccccaggtcc ggctgggcac gcgccccgcc gtcggccttg
     3181 tcaccctgcg ctgaggcccc cggaggccca gccccacccc gaccccctgg aacgaacttc
     3241 gtaggccgga gcgcccgatt tggggctctc gcctacgaag ttcgggaggg gtggggcggc
     3301 cccgggtgcg tcagccggcg aggaaggcgg cgacgtcttc gggagcgacg atgttgatgg
     3361 ggacgccggt gccctgctcg aacccgccgg tgctgtggac ccgcggcgtg atgtgggcgt
     3421 gccagtggaa cgggtcgccg gcacggtggg ggagcgtgtg gaagacgatg ttgtacgaca
     3481 cgtcgcccag cagggtgcgg aggcgggcca gggcgtcgcg gatcgcggtg ccgacggcca
     3541 cgagcgagtc cggcttggcg tggtgcaggt ggccctcgtg ctggacgggg atcacgagca
     3601 gctcgaacgg cgatccgctc caccacgggg cgatcacggc gacgtcgtcg tcgatggcga
     3661 tcgtgcggta gccggcgtcg cgctcggcgg cgagggtggc gcacagcagg cagccgcctt
     3721 ggaaccggcg gaaaccggcc agctcctcga tgagctcgcc cgggacgaac gggatgccca
     3781 gcagctggcc gtgcgggtgg cggcggctcg cgcccgctgc gaggccgtgg ttcacgatgg
     3841 cctggctgta gcggatgccc gcggtggcgg cgtggtcctc gagccggtcg cgcagggcgg
     3901 cc
//
LOCUS       DATMFS010000019         3592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_302980, whole genome shotgun sequence.
ACCESSION   DATMFS010000019 DATMFS010000000
VERSION     DATMFS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302980"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..923)
                     /locus_tag="VNQ33_00360"
     CDS             complement(216..923)
                     /locus_tag="VNQ33_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96574.1"
                     /translation="MEKVLYVIAHPVGAVPADLTKHLVGPLGDELQGLGARGVQVNVA
                     DHDVSAAGGLRMAMSPSPANAMVSVWVDSATDHLRAPFDAAVRRVAEGAEVSAYLVTE
                     SVPLANTTYPAAPGGRTPGMAQLAFLRRPEGMAVDHWLEVWLGSHTPIALELQDTFAY
                     VQNVVVRTLVAGTTAWHAIVEECFPAAAMSDPHVFFDAVGDDEKLVRRQREMFESVQR
                     FIDLSFIDVVPTSRYLL"
     gene            1070..1675
                     /locus_tag="VNQ33_00365"
     CDS             1070..1675
                     /locus_tag="VNQ33_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ96575.1"
                     /translation="MAFPRIARALAGTVLLVSGLAGCGLFDRDAGDRVLVVGDSVTYQ
                     SRAYLVKEFAWADELDIEATSGLRTDQLLPDAEEGLSHHPSSAAFMPGYNDILQDRVD
                     RAALPEMMDLAAKVPCSVWLLIPVKGVYAPATAEAWNQRVRDEAAGHDNVHLVDDWAR
                     LVDNSQDYALVKSEDAVHPNGLGRKAVADVMSKSIQRECGA"
     gene            complement(2106..2717)
                     /locus_tag="VNQ33_00370"
     CDS             complement(2106..2717)
                     /locus_tag="VNQ33_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ96576.1"
                     /translation="MPRGRGGRPARSNPPIHGDEDLTQLAVRAAAGDRAAEEAFVART
                     IDDVWRYCRHLVGPAHADDATQAAYLRALRSLGSFRGESSAKTWLIGVARNTCLDLRR
                     TLARRSALDERLEAQPRPTGRDLHDDHLPAELDELLAELSTQRREAFVLTQLLGFPYD
                     AAAEVIGVPIGTIRSRVARARGDLLALVGTDDGRDPIGGVASV"
     gene            2841..3452
                     /locus_tag="VNQ33_00375"
     CDS             2841..3452
                     /locus_tag="VNQ33_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWJ96577.1"
                     /translation="MEHPEWLELISAEADGELDDVESARLADHLQRCPACTALLTQFE
                     ATRRRARIRTGPRRGTLVGAVLDARGDDHREHDRARVVLLRRCAVAASGLAAAVAALV
                     MITSGSTPLVPVRPQHDHDALIAARDRSFDRADIEVEAGTTVEWRNAGTRTHHLVREF
                     DGVTIDEDLPPGTTETATFDRPGTFPYFCTIHPEMAGTVTVDA"
BASE COUNT          513 a         1325 c         1313 g          441 t
ORIGIN      
        1 caggcggcga ggaaggcggc gatgacggcc acgagcaccc ccacggcccg cggcgcgcgt
       61 cggcgtcgac cgctcggacg gcgcggtgca ggacggggag cgatcagaga cacagcggac
      121 catcgtcgcg cgccagccca acagggcgga cgcggccgaa acggcgggtg gcggtcagac
      181 gcgcgccgtc cgtgcacgcg gcgcaacccg cggggtcaca gcaggtagcg gctggtgggg
      241 acgacgtcga tgaacgacag gtcgatgaag cgctggacgc tctcgaacat ctcgcgttgg
      301 cgccggacga gcttctcgtc gtctccgacc gcatcgaaga agacgtgggg atccgacatc
      361 gccgccgcgg ggaagcactc ctccacgatg gcgtgccagg cggtggtgcc ggcgacgagg
      421 gtgcgcacca ccacgttctg gacgtaggcg aacgtgtcct ggagctcgag ggcgatgggg
      481 gtgtggcttc cgagccagac ctcgagccag tggtcgacgg ccatgccctc aggccggcgc
      541 aggaaggcca gctgcgccat ccccggcgtc cgcccgccag gggcggccgg gtaggtggtg
      601 ttggccagcg ggaccgactc ggtcaccagg taggccgaga cctcggcacc ctcggccacg
      661 cgccgcaccg ccgcgtcgaa cggcgcccgg aggtggtcgg tggcgctgtc gacccagacc
      721 gacaccatcg cgttggcggg cgacggcgac atcgccatcc gcagcccgcc tgccgccgag
      781 acgtcgtgat cggccacgtt gacctgcacg ccgcgggccc cgagcccctg cagctcatcg
      841 ccgagcggcc cgacgaggtg cttggtcaag tcggccggaa ccgcgcccac cggatgggcg
      901 atcacgtaga gcaccttctc catgacctgg gaccgtaccc gtgggcgtcc gcccacgccg
      961 ggtgatgccg gcgcgtggcg ccacccgcac ggaccaggtt cggcggtgac gaggccggga
     1021 tcgaccaggc gacccgtctc gaacgggccg cgccgggata ctgggtgaca tggcgttccc
     1081 ccgcatcgct cgtgccctcg ccggcacggt cctcctcgtg agcggcctgg ccggctgcgg
     1141 gctcttcgac cgcgacgccg gcgaccgcgt cctcgtcgtc ggcgactcgg tgacctacca
     1201 gtcccgcgcc tacctggtga aggagttcgc ctgggccgac gagctcgaca tcgaggcgac
     1261 gagcgggctc cggaccgatc agctgctgcc cgacgccgag gagggcctct cgcaccaccc
     1321 gtcgagcgcg gccttcatgc ccggctacaa cgacatcctc caggaccgcg tcgaccgggc
     1381 cgcgctgccc gagatgatgg acctggcggc gaaggtcccg tgctcggtct ggctgctgat
     1441 cccggtgaag ggcgtgtacg cgcccgccac cgccgaggcg tggaaccagc gggtgcgcga
     1501 cgaagccgcg ggccacgaca acgtgcacct ggtggacgac tgggcccgcc tcgtggacaa
     1561 ctcgcaggac tacgccctgg tgaagtccga ggatgcggtc cacccgaacg ggctcggccg
     1621 caaggccgtg gccgacgtca tgtcgaagtc catccagcgc gagtgcggcg cctgacgggc
     1681 gcatcgatcc cggcgcgcgg cccgcaggtc ggagcaccgg gcaccatcag gtcaagcacg
     1741 ggctccgagc ggccgatcat catggtgacg cgccgcgaac acggaggaac gacgtgccat
     1801 cgcccggtgg gactcggggg accacatctt cgaagaggtg tgcttcgtgc cgggtccggg
     1861 cagcgagccg ggcgtcggct tctacgtgac gttccgcacc gatcgcacca cgctcgagag
     1921 cgacgtcgtg ctcgtggccg cggacgacgt ggccgccggc cccgtggccc gcatccccct
     1981 gccccaccgc gtcccgtgcg gcctccacgg gactggttcc cagcaccctg accgagccgc
     2041 tcgtgccgcg gcggaaaccg ccggaccagc ggaccagcgg accagcggcc tgcccgtgcg
     2101 tctggtcaga cggaggcgac gccgccgatc gggtcgcgtc cgtcgtcggt gccgaccagg
     2161 gcgaggaggt cgccgcgggc gcgcgccacg cgcgaccgga tcgtgccgat gggcacgccg
     2221 atcacctcgg ccgcggcgtc gtaggggaag ccgaggagct gcgtcagcac gaaggcctcc
     2281 cgccgctggg tgctgagctc cgccagcagc tcgtcgagct cggcggggag gtggtcgtcg
     2341 tggaggtcgc gccccgtggg ccgcggctgc gcctcgaggc gctcatcgag agccgagcgg
     2401 cgggcgaggg tgcggcgcaa gtcgagacag gtgttgcggg ccacgccgat caaccaggtc
     2461 ttggccgagg actccccgcg gaagctgccg agcgaacgca gggcgcgcag gtaggcggcc
     2521 tgggtggcgt catcggcgtg ggcgggaccg accaggtgcc ggcagtagcg ccagacgtcg
     2581 tcgatggtgc gggcgacgaa cgcctcctcg gccgcccggt cgcccgccgc ggcccgcacc
     2641 gcgagctggg tgaggtcctc gtcgccgtgg atcggcgggt tcgatcgggc cggccggccc
     2701 ccgcgtcccc ggggcatgga cgacgaccgt accggtgggc tggcgccggc gcgcaatgga
     2761 ctcggccgtt ccgggcgccc ggttcgccgg ggtccatcgg ccgggaacca ccggacggtt
     2821 cgcgccgacc catctcacgc atggaacacc cggagtggct cgaactgatc tcggcagagg
     2881 ccgacggcga gctcgacgac gtcgaatcgg cgcgcctcgc cgatcacctc cagcgctgcc
     2941 ccgcctgcac cgcgctcctg acccagttcg aggccacccg gcggcgggcc cgcatccgca
     3001 ccggaccccg gcgcggcacg ctggtcggtg cggtcctcga cgcccgaggc gacgaccacc
     3061 gcgagcacga ccgggcccgg gtcgtgctgc tgcgccgctg cgccgtggcg gccagcggcc
     3121 tcgccgcggc ggtcgccgca ctggtcatga tcacctcggg cagcaccccg ctggtaccgg
     3181 tcaggcccca gcacgaccac gatgcgctca tcgcggcacg ggaccgctcc ttcgatcggg
     3241 ccgacatcga ggtcgaagcc gggaccaccg tcgagtggcg caacgccggt acccgcaccc
     3301 accacctggt ccgcgagttc gacggcgtca ccatcgacga ggacctgccc ccgggcacga
     3361 ccgagaccgc cacgttcgac cgcccgggaa ccttccccta cttctgcacc atccacccgg
     3421 agatggccgg caccgtcacc gtcgacgcgt gaccggcgcg cccttcgcgc tcagccgacg
     3481 cggtcttcga gccagcgccg gatgcggacg ggtgtctggg ggaccttctc ggccacctgg
     3541 ccggccaagc cggcgatggt ctcggccgcg agctcggcgc gccacgcggc ct
//
LOCUS       DATMFS010000020         3632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_47082, whole genome shotgun sequence.
ACCESSION   DATMFS010000020 DATMFS010000000
VERSION     DATMFS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47082"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..505)
                     /locus_tag="VNQ33_00380"
     CDS             complement(326..505)
                     /locus_tag="VNQ33_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWJ96578.1"
                     /translation="MARMVADCRDFPSESGCTLTIAGEEDEVVAAAAIHAATVHGHED
                     SPELRETLRSSLKPE"
     gene            complement(547..843)
                     /locus_tag="VNQ33_00385"
     CDS             complement(547..843)
                     /locus_tag="VNQ33_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96579.1"
                     /translation="MGFVQIIELQTTRFEEVDALHETWLAETEGIRRTVSELVVKDRD
                     KPGRYLVIVEFASYEDAEANNDIPATGRFAEALGPLLDAPPIFHNLDLVRYDRT"
     gene            complement(943..2604)
                     /locus_tag="VNQ33_00390"
     CDS             complement(943..2604)
                     /locus_tag="VNQ33_00390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWJ96580.1"
                     /translation="MTGPAWFDRTEALRRLGGDPFDVLVVGGGITGVGAALDAASRGL
                     RTALIERDDFASGTSSKSSKLAHGGLRYLQQGEVRLVYEALAERQRLRHNAPHLVKLL
                     PFLLPVFSGSGGVINKKLARALGASMWMYDLTGGARIGKFHERLSPDEALAHMPTLPK
                     DKIASSYLYYDATVDDARLSLTVARTAAIEFGAVAVNGTSLTGIAKDGQGRVSGATVV
                     ADGQEISVRCQAIVNAAGVWSDEVRTLDEGTDPDSIRPAKGIHITVPWSKIRNDIAVV
                     VPVPKDKRAVFVVPWGDLTYIGTTDTDYQGPHEDPPVTREDVDYLLDAMKFTGITDHD
                     VVGSWAGLRPLVKSASSGRTADLSRRHKVVASPSGVVSVTGGKLTTYREMSADTVDEV
                     VEVLGSRVSGIARGSRTKKLRLRGAEGYDSLAASTDALTSHLANRYGGEARTVLALAD
                     SDPALREPLVAGLPYIRAEAVYAVRHEMARSVDDVLSRRTRARLLARDASAAAAADVA
                     SLIAPILGLSESEAEAQASSYRAAVELERSTAQLPETALDASLGA"
     gene            2760..3497
                     /locus_tag="VNQ33_00395"
     CDS             2760..3497
                     /locus_tag="VNQ33_00395"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWJ96581.1"
                     /translation="MDDAGAAEGESFAAAWYPDPDGRHEQRYFDGKVWTERVRTAGVE
                     RTDLLDGSYLAPGAASTMAAFYPGRPASSLGARSVARNAPGAGLLLAGIGWVVMVGCT
                     RFPWNVGVALFALEPQTGSPTSADAVSQLQGQVAVSLGLLVLVIVGTTLRYQPGKVYG
                     FCMGGCFGLIVCWRKLDRDATSIRANLLGAFVVTVQGVVIGARAHAVTRSYEHVRLGS
                     GVVGFSIGMLLVLLGVLVGRRRVLGAG"
BASE COUNT          477 a         1218 c         1386 g          551 t
ORIGIN      
        1 aacagctcgg ggatcagcac cacctgggcg ccctgcgcgg cggcgtcgcg gatgagcgcc
       61 tcggcggccg cgacgttggt ggcgacgtcg tcggccatcg acatctgcac cgacgcaacg
      121 gtcacgcttc gcacggggga ccacctcgct cagatcgaca ggacctcagc ggacggtacc
      181 gccgcatcac caccgccgag acgatttcgg gtcacgcgtg caccatccgg gtgcccaggt
      241 gacccgaaat cgatggggga tccggaccag ctctggtttc gggtcacgcg tgcaccgcca
      301 gggtgctcag gtgacccgaa atcggctact cgggtttcag ggaggagcgg agcgtctcgc
      361 ggagctcggg tgagtcctcg tggccgtgga cggtggcggc gtggatggcg gctgcggcca
      421 ccacctcgtc ctcctcgccc gcgatggtga gggtgcagcc gctctcgctc gggaagtccc
      481 ggcagtcggc aaccattcgg gccatgacgg ccccctttcg tcgggcgcct cgcggggagg
      541 cgcgcgtcag gtgcggtcgt accgcacgag gtccaggttg tggaagatcg gcggcgcgtc
      601 gaggagcggg ccgagggcct ccgcgaagcg gcccgtcgcc gggatgtcgt tgttcgcctc
      661 ggcgtcctcg tagctcgcga actcgacgat cacgaggtag cggccgggct tgtcgcggtc
      721 tttgaccacc agctcgctga cggtcctgcg gatgccctcg gtctcggcga gccacgtctc
      781 gtggagcgcg tcgacctcct cgaagcgagt cgtctgcagc tcgatgatct gcacgaaccc
      841 catcagaacc ctccccggtc tggtggcacg tccgtcgcat cgtcgcgccg aagcgacgtt
      901 ccgtcgaggg aaacctgccc caccccggcc ggcgaggtgg ggctacgccc cgaggctggc
      961 gtcgagtgcg gtctcgggga gctgggccgt gctgcgctcg agctcgaccg cggcccggta
     1021 gctggacgcc tgcgcctcgg cctccgactc ggagagaccg aggatcgggg cgatgaggga
     1081 ggcgacgtcg gcggccgcag cggccgacgc gtcgcgggcc aggagccggg cccgggtgcg
     1141 gcggctgagc acgtcgtcga ccgagcgggc catctcgtgg cggacggcgt agacggcctc
     1201 ggcccggatg tacgggaggc cggccacgag cggctcgcgc agggcggggt ccgagtcggc
     1261 gagggcgagc accgtccggg cctcgccgcc gtagcggttg gcgaggtgcg acgtcagggc
     1321 gtcggtggac gctgcgagcg agtcgtaccc ctcggcgcct cgcaggcgga gcttcttggt
     1381 gcgcgagccg cgggcgatgc cgctgacccg gctgcccagg acctcgacga cctcgtcgac
     1441 ggtgtcggcc gacatctccc ggtacgtcgt gagcttgccg ccggtgaccg acacgacgcc
     1501 gctgggcgag gcgacgacct tgtgccggcg cgacaggtcg gcggtgcggc cggacgaggc
     1561 gctcttcacc agcgggcgga ggccggccca gctgcccacg acgtcgtggt cggtgatgcc
     1621 ggtgaacttc atggcatcga gcaggtagtc gacgtcttca cgggtgaccg gcggatcctc
     1681 gtgggggccc tggtagtcgg tgtcggtggt gccgatgtag gtgaggtcgc cccagggcac
     1741 cacgaacacc gcacgcttgt ccttcgggac gggcaccacg acggcgatgt cgttgcggat
     1801 cttcgaccac gggacggtga tgtggatgcc cttggccggt cggatcgagt ccgggtcggt
     1861 gccctcgtcg agggtgcgga cctcgtcgga ccagacgccc gctgcgttca caatggcttg
     1921 gcagcggacc gagatctcct ggccgtcggc cacgacggtg gcgcccgaga cccggccctg
     1981 accgtccttg gcgatgccgg tgagcgaggt gccgttgacg gcgacggcac cgaactcgat
     2041 ggccgcggtg cgggcgacgg tgaggctgag gcgggcatcg tcgacggtgg cgtcgtagta
     2101 caggtacgat gaggcgatct tgtccttcgg cagcgtcggc atgtgggcca gtgcctcatc
     2161 gggcgacagg cgctcgtgga acttgccgat gcgggcgccg ccggtgaggt cgtacatcca
     2221 catggaggca ccgagcgccc gggcgagctt cttgttgatg acgccaccgg acccgctgaa
     2281 cacggggagc aggaacggga gcagcttcac caggtgcggc gcgttgtggc gcaggcgctg
     2341 gcgctcggcc agggcttcgt acacgagccg gacctcgccc tgctgcaggt agcgcaggcc
     2401 gccgtgggcc agcttcgacg acttcgacga cgtgcccgac gcgaagtcgt cgcgctcgat
     2461 gagcgcggtg cgcaggccac gggacgcggc gtcgagcgcc gcgcccacgc cggtgatgcc
     2521 gccgcccacg acgaggacgt cgaacgggtc gccgccgagg cggcgcaggg cctcggtgcg
     2581 gtcgaaccac gcggggccgg tcatgaggag gctcgagcgt cgagtggggt gcgcatggac
     2641 cccgatccta cggtgagacc cccgtcagcg ggccaccggc gtgtcgcatg accgcgccgg
     2701 cgccgcccta gagccgtgct ccgccagggg gagccgctgg ggctggggag gtgcgcaagg
     2761 tggacgacgc gggagcggcc gagggcgaga gcttcgccgc cgcgtggtac ccggaccccg
     2821 atggccgcca cgagcagcgg tacttcgacg ggaaggtgtg gaccgagcgg gtgcgcaccg
     2881 cgggcgtcga gcgcaccgat ctcctcgacg ggtcctacct ggcgcccggc gccgcgtcga
     2941 cgatggcggc gttctatcca ggcaggccgg catcgtcgct cggcgctcgg tccgtggcgc
     3001 gcaacgctcc gggggccggc ctcctgctgg cgggcatcgg ctgggtcgtg atggtcgggt
     3061 gcacccggtt cccctggaac gtgggggtgg ccctgttcgc cctggagccc cagaccggct
     3121 cgccgacgtc ggccgatgcc gtgtcccagc tccagggcca ggtggcggtc agcctgggcc
     3181 tgttggtcct cgtgatcgtc ggtaccaccc tgcggtacca gcccggcaag gtctacgggt
     3241 tctgcatggg cggctgcttc ggcctgatcg tgtgctggcg gaagctcgac cgggatgcga
     3301 cctcgatccg cgccaacctg ctcggcgcct tcgtggtgac cgtccagggc gtcgtgatcg
     3361 gggcgcgtgc gcacgccgtc acccggtcct acgagcacgt ccgcctcgga tcgggcgtcg
     3421 tcgggttctc gatcgggatg ctgctcgtgc tgctcggcgt gctcgtcggc cgccggcgcg
     3481 tcctcggcgc cggctgaccg ctgccgggca tcgcccggat cctgacggga acctgacgat
     3541 ccagcatcgg cggtggcggg tggattcgac cgatctccac cgagaacctc gtcttcggtg
     3601 tggacacccc cggaggcggg cgaccatact ca
//
LOCUS       DATMFS010000021         3308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_47137, whole genome shotgun sequence.
ACCESSION   DATMFS010000021 DATMFS010000000
VERSION     DATMFS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47137"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VNQ33_00400"
     CDS             <1..1726
                     /locus_tag="VNQ33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWJ96582.1"
                     /translation="MDSPIIFSSEMPRSTISRRQSDGTLLRIARGIYSTDVHTSPEDQ
                     VNRHWMTVVAHEVPGAVIVDRSAPVGRPRDGHLFVDAGPGPKRRPIELPGLTIIRRSG
                     PGPLDGDMPLRDGLMLSSQARALADNSRRSRAAGGRPPATLTDDEIADWIADLATRAR
                     PAAIGRLRDEAERLAEQTGTTATIGRVSELLGAALGTRTVDTGSRALGARQAGRPYDQ
                     QRVDKFAVLVDHLSGQAPSPVEAAAPARAVHLPFFEAYFSNFIEGTVLTLDEAEGVMY
                     RDEDLPTHPEDAHDVAATFRLAADPDEARQVPASADELIAILRHRHATLMAARTDKHP
                     GQFKGARNQVGRYVFVHHDQVEGTLQAGWDCLDQLDDPFQRAVAMMYVVTEVHPFDDG
                     NGRVARLMMNAELTAAGESRLIIPPVYRGEYLASLSTMSTTTDRTDSTACSASLNGGP
                     RSSTSRRSEQPCATSKQPMPSSTRSPPTRRGSSCGSSPRYRPTNSNEPPNPKLHLVDG
                     TGATAWAGSRSRTGGRYLRASSGGGRSAGPPPLPGLSGSGRGAAGTAGRTRAGEGPKA
                     VPAVTGLW"
     gene            complement(1974..>3308)
                     /locus_tag="VNQ33_00405"
     CDS             complement(1974..>3308)
                     /locus_tag="VNQ33_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96583.1"
                     /translation="ASAATSSDLLYSTDGGASWSSSVTVAPGGTVLVRQWYDNNGSAA
                     ETGASLSTTLPGGFSLNGGSTKVCLNPSTSSPASPNGSELVCASSNEGAVWSGADLQV
                     SPTAGLFGQSNGQTSGILEMGKKRYLNLHQCGYNRLDPGMTEYVTTFAQLSGSYSAGT
                     NASNTADAAPTCGPGNVTNLLPTATLTSNMALDLMGQRYVNLNQCGYRRLDPGMTEYA
                     NTFAQVSGNYSAGTNASNTADAAPTCGPGNPANLEPPSSLTRNLALDLLGKRYVNLHQ
                     CGYNRLDPDMTEYITTGVATPGNYSAGTNASNTADAAAVCGPGSGAPDFLAQTASLTA
                     VKALDLLDTARGHGYVEYALTAPPGPTPEACAADPSAGSEAFAQDASLTSTPSGAKAS
                     SGTITVDWSLLSEPPCPEDPIPLVDPVVAGAGLVAVAGAGFVLYRRRETAVA"
BASE COUNT          584 a         1230 c         1046 g          448 t
ORIGIN      
        1 gatggacagc ccgatcatct tcagcagcga gatgccccgc tcgaccatct cacgccggca
       61 gagcgacggc acgctgctgc gcatcgcccg gggcatctac agcaccgacg tccacacctc
      121 gcccgaggac caggtgaacc ggcactggat gacggtcgtc gcccacgagg tccctggcgc
      181 ggtcatcgtc gaccggtctg cgccggtcgg ccgtccccgc gacgggcacc tcttcgtcga
      241 cgcgggcccc ggacccaagc gccgacccat cgaactgccc gggctcacga tcatccgacg
      301 aagcggcccc ggtccccttg acggcgacat gccgctccgc gacggtctca tgctgtcatc
      361 gcaggctcga gcactggccg acaacagccg acggtcccgg gccgcgggcg gacgcccccc
      421 ggcgacgctc accgacgacg agatcgctga ctggatcgcg gacctggcca cacgagcccg
      481 acccgcggcc atcgggcgcc tgcgcgacga agccgagcgc ctcgccgagc agaccggaac
      541 gacggcgacc atcgggcggg tcagcgagct gctcggcgcc gccctcggta cacgaaccgt
      601 cgacaccgga tcccgtgcgc tcggagcccg ccaggccggc cgcccctacg accagcagcg
      661 ggtcgacaag ttcgccgtcc tcgtcgacca cctgagcggc caggccccgt cgccggtcga
      721 ggcggcagcg ccagcccgag ccgtgcacct cccgttcttc gaggcgtact tctcgaactt
      781 catcgaaggc accgtgctga ccctcgacga agccgaaggg gtgatgtacc gcgacgagga
      841 cctgccgacc caccccgaag acgcccacga tgtggccgcc accttccggc tggcggcaga
      901 ccccgacgaa gcccgacagg tcccagcatc ggccgacgag ctcatcgcca tcctccgaca
      961 ccgccacgcc accttgatgg cagcacgcac cgacaagcac cctggccagt tcaagggcgc
     1021 caggaaccag gtcggccgct acgtcttcgt ccaccacgac caggtcgaag ggacgctgca
     1081 ggccgggtgg gactgcctcg accagctcga cgacccgttc cagcgagcgg tggcgatgat
     1141 gtacgtcgtc accgaggtcc accccttcga cgacggcaac ggacgggtcg cccggctgat
     1201 gatgaacgcc gagctcaccg cagccggcga gagccgcctc atcatcccac ccgtctaccg
     1261 aggcgagtac ctggcctcgc tcagcacgat gtcgacaacg accgaccgga cggactcaac
     1321 cgcgtgctcg gcttcgctca acggtggacc gcgcagctcg acttctcgac gctccgaaca
     1381 gccgtgcgcg acctcgaagc aacccatgcc ttcgtcgacc cggtcacctc ccacacggag
     1441 ggggtcaagc tgcggctcat ctcctcggta ccggcctacg aactcgaacg agccaccgaa
     1501 cccgaagctc cacctcgttg acggcaccgg tgccaccgcg tgggctggct cacggtcacg
     1561 aactggtggc cggtacctcc gagccagttc cggcggaggc aggtccgcag ggccaccccc
     1621 tcttccgggc ctttccggct cggggcgcgg ggcagccggg accgctggcc ggacccgggc
     1681 gggagagggt ccgaaagcgg tcccggctgt caccgggttg tggtgaggag cggcccccaa
     1741 gctgatccag gtcgtgcctc ctgggctcga tcccgggcac tgccagctcg cttacctcaa
     1801 attcgtgccg gtgggccgga gcccgggaac agggaagaac acgaacccag cccaccggtc
     1861 gccgcgttcg gcccaccggc cagagggctg cgcttcgtca tcgagtcggg cacgacctcg
     1921 accacaccgc agacccccag ccgacagacc gggcgaacaa gggaaaccgg gggtcaggcc
     1981 accgccgtct cacggcgccg gtacagcacg aagcccgcac cagccaccgc cacgaggccg
     2041 gcaccggcga ccacggggtc gaccagcggg atcgggtcct ccgggcacgg cggctccgac
     2101 agcagcgacc agtccacggt gatcgtcccc gacgacgcct tcgcacccga cggcgtgctg
     2161 gtcagcgacg cgtcctgcgc gaacgcctcc gaaccagccg acgggtccgc cgcacacgcc
     2221 tccggcgtcg gcccaggagg agccgtcaac gcatactcca catacccatg accccgagcc
     2281 gtatccaaca gatccagcgc cttgaccgcg gtgagcgagg ccgtttgcgc aaggaagtcg
     2341 ggagccccgc tgccggggcc acagacggca gccgcatcgg cggtgttcga cgcgttcgtg
     2401 cccgcggagt agttgccagg ggtcgcaacg ccggtggtga tgtactccgt catatctgga
     2461 tccagacggt tgtaaccgca ctggtgcagg ttgacgtagc gcttgcccag caggtcgagc
     2521 gccaggttcc tggtgagcga agacggcggc tcaaggttgg ccggattgcc gggaccacag
     2581 gtgggagccg cgtcggcggt gttcgaggca ttcgtgcccg ccgagtagtt gccggagacc
     2641 tgagcaaacg tgttggcgta ctccgtcatc cccggatcca gacgccgata gccgcactgg
     2701 ttcaggttca catagcgctg acccatcaga tcgagcgcca tgttgctggt gagcgtcgcc
     2761 gtcggcagga ggttggtcac attgcccggg ccacaggtgg gagccgcatc ggcggtgttc
     2821 gacgcgttcg tgcccgcgga gtagctgccg gagagctgag caaacgtcgt gacgtactcc
     2881 gtcatccccg gatccagacg gttgtagccg cactggtgga ggttgaggta gcgcttcttg
     2941 cccatctcca agatgccact ggtctggccg ttcgactgac cgaacagacc cgccgtcggc
     3001 gacacctgca gatccgcacc ggaccacacc gcaccctcgt tcgaagatgc acacaccagc
     3061 tccgaaccgt tcggagaagc cgggctcgac gtcgacgggt tcaagcacac cttcgtcgac
     3121 ccgccgttca aactgaaccc accaggcagc gtcgtcgaca gcgacgcccc cgtctccgcc
     3181 gcactcccgt tgttgtcgta ccactgccgc accaacaccg tgccgcccgg cgccaccgtc
     3241 accgacgaag accagctcgc gcccccatcg gtcgaataca gaagatcaga agacgtagcg
     3301 gcggacgc
//
LOCUS       DATMFS010000022         4131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_233236, whole genome shotgun sequence.
ACCESSION   DATMFS010000022 DATMFS010000000
VERSION     DATMFS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233236"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VNQ33_00410"
     CDS             <1..304
                     /locus_tag="VNQ33_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ96584.1"
                     /translation="ITLIHTPGHTPGSQCFLVDGKLVAGDTLFLNGCGRTDLPGSDPE
                     QMYYSLTQRLGQVPDDAVLYPGHLYSEKPSATMAETRSRNVVFRPGSLEQWLMMFT"
     gene            complement(450..2369)
                     /locus_tag="VNQ33_00415"
     CDS             complement(450..2369)
                     /locus_tag="VNQ33_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWJ96585.1"
                     /translation="MTSPFKRLLVGKPIATSEEAHQRIGKPVALAVFASDAVSSTAYA
                     TEEMMLVLLTAVMFPQALDYLVPLSIAAVILLVVVGISYIQTIHAYPDGGGAYVVSRE
                     NIGRTASLVAGGSLLVDYTLTVAVSISSGVLAIGSAFNFNDNATARIGLALFFVAMMC
                     VGNLRGLKESGKVFAVPTYFYVCMLTIFLGVGFFKLVTGNLHVLAGSAELAEHFDKNH
                     DLVAGATLFVLLRAFSSGAVVLSGVEAISNGVPAFKKPESRNASQTLAMMAGILAVGF
                     LGISTLAHHMKPVVDEGGQTVLAQMAAGVFGGHNLFFYGLQFATFAILVMAANTAYAD
                     FPRLSSLIARDGYLPRQLANRGDRLVFSNGILALSAMAAVLIVVFKAEVSALIPLYAV
                     GVFTGFSLSQFGMIKHHLTLREEHWRRGLAINAVGCLATTVVLAVVVISKFTIGAWIP
                     VVVIPIIVAVFLRIHRHYVKIDKRMAAKPGEKVRRRTNTVVVLVGKVTKGSLTAIAYA
                     RSLNPDRLVAVTVVSTPEEQERITRQWEEHQIPVELRELYSPYRELSRPIMSFIDELD
                     AAHGDDFVTVIVPEFALEHWWQQLLHNQSALVLRTRLRNRPNTVVTSVPFHVDDDDRS
                     ADFGSSVHGRGAQAT"
     gene            complement(2412..>4131)
                     /locus_tag="VNQ33_00420"
     CDS             complement(2412..>4131)
                     /locus_tag="VNQ33_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWJ96586.1"
                     /translation="AVILLAVVSTSYRQTIYAYPDGGGAYIVSRDNISQTAGLVAGAS
                     LLVDYTLTVAVSVSAGTLAIGSAFHFNDNATLRVGLALFFVALLAVGNLRGLKEAGKI
                     FAVPTYAYTVLLGGLIAYGMYKIHFQHLGTIPGNEKLAHDFATAHGADLKTLTGSVGL
                     ILLLRAFSSGAVVLAGVEAMSNGVPSFRKPRSKNAAQTLVMMAVILGTLTLGVSYVAS
                     HLHPVFVEDGDTVLSQLGGHIFGSGTVLYYALQFATFAILILAANTAYNGFPQLSSNI
                     ARDGYLPRQLANRGDRLVFSNGVLILSGMAGVLIVAFQAQVSALIPLYAVGVFVGFTL
                     SQFGMCRHHLRLREPKWKGGLAVNALGCAMTFTVLMVVIISKFTEGAWIPVLVIPAVV
                     VLLQGINRHYRSMDELLVIEPDEKVRRKTNTVIVLVARVSRGSLIALAYARSLHPDRL
                     VAVSVVSNPEEQDRITEAWERHQIPVELQTLYSPYRELSRPIMTYIDELDDRHDDDFL
                     TVIVPEFTLDKWWQQLLHNQSALVLRNRLRNRPNTVVTSVPFHADRKVAGGADAGPAA
                     PADLTT"
BASE COUNT          719 a         1327 c         1468 g          617 t
ORIGIN      
        1 catcacgctc atccacacgc cgggccacac cccgggcagc cagtgcttct tggtggacgg
       61 gaagctcgta gcgggcgaca ccctgttcct gaacggctgc ggccggaccg acctccccgg
      121 cagcgacccg gagcagatgt actactcgct cacccagcgt ctcgggcagg tccccgacga
      181 cgccgtcctc taccccggcc acctctactc cgagaagccc tcggccacga tggccgagac
      241 ccgcagccgc aacgtcgtct tccgccccgg ctccctcgag cagtggctga tgatgttcac
      301 ctgacccact gggccgtcgg tagcacggcc gtcctcgtag caggcgtcct gagcatcgtc
      361 cgggttgccc ccccttcgga tcgctcgctt cgagcgtggt tcccacggac cgtggccgga
      421 atcggacgtc gcgggccttc caggcgattt caggtcgcct gtgcaccgcg accgtgcaca
      481 gatgacccga aatcagctga tcggtcgtcg tcatcgacgt ggaacggcac gctcgtgacc
      541 accgtgttcg gccggttgcg cagccgggtt cggaggacga gcgcgctctg gttgtggagg
      601 agctgctgcc accagtgctc gagggcgaac tcggggacga tcaccgtcac gaagtcgtcg
      661 ccgtgggcgg catcgagctc gtcgatgaag ctcatgatcg ggcgcgacag ctcgcggtag
      721 ggcgagtaca gctcacggag ctccaccggg atctggtgct cctcccactg ccgggtgatg
      781 cgctcttgct cctcgggcgt ggacaccacg gtgacggcca cgagccggtc ggggttgagc
      841 gaccgggcgt aggcgatggc cgtgagcgag cccttggtga ccttgccgac gaggaccacg
      901 acggtgttgg tgcggcggcg caccttctcg ccgggcttgg ccgccatgcg cttgtcgatc
      961 ttcacgtagt ggcggtggat ccgcaggaag accgccacga tgatcgggat caccaccacc
     1021 gggatccagg cgccgatcgt gaacttcgag atgacgacca cggcgagcac gacggtggtg
     1081 gcgagacagc cgacggcgtt gatggccagc ccccgccgcc agtgctcctc gcgcagggtg
     1141 aggtggtgct tgatcatgcc gaactggctc agcgagaacc cggtgaacac gcccaccgcg
     1201 tacagcggga tcagcgccga cacctcggcc ttgaagacga cgatcaagac cgcggccatg
     1261 gccgacaggg cgaggatgcc gttcgagaac acgagccggt cgccgcggtt ggcgagctgc
     1321 cggggcaggt agccgtcgcg ggcgatcagc gagctgagcc gagggaagtc ggcgtaggcc
     1381 gtgttggcgg ccatgacgag gatggcgaac gtggcgaact ggagtccgta gaagaagagg
     1441 ttgtggccgc cgaagacccc cgctgccatc tgggcgagca cggtctggcc gccctcgtcg
     1501 accaccggct tcatgtggtg ggcgagcgtc gagatgccca agaagccgac cgccaagatg
     1561 ccggccatca tcgccagggt ctgcgaggcg ttccgtgact cgggcttctt gaacgccggc
     1621 accccgttgg agatggcctc gacgcccgac agcacgaccg cacctgagct gaacgcccgg
     1681 agcagcacga agagcgtcgc cccggccacg aggtcgtggt tcttgtcgaa gtgctcggcc
     1741 agctcggcgc tgcccgccag cacgtggagg ttgccggtga cgagcttgaa gaagccgacg
     1801 cccaagaaga tcgtcagcat gcacacgtag aagtacgtgg gcacggcgaa caccttgccg
     1861 gactccttca gcccccgcag gttgcccacg cacatcatgg ccacgaagaa gagcgccagg
     1921 ccgatgcggg cggtggcgtt gtcgttgaag ttgaacgccg agccgatggc gagcacgccc
     1981 gaggagatcg acacggcgac ggtcagcgtg tagtcgacga gcagcgagcc gccggccacc
     2041 aacgacgccg tgcggccgat gttctcccgg ctcaccacgt acgcgccacc gccgtcgggg
     2101 taggcgtgga tcgtctggat gtagctgatg cccaccacca ccagcaggat caccgcggcg
     2161 atcgacaacg ggaccaggta gtcgagggcc tgcgggaaca tgaccgccgt caacaagacg
     2221 agcatcatct cctcggtcgc gtacgcggtc gacgagacgg cgtcagaggc gaacacggcg
     2281 agggcgacgg gcttgccgat gcgctggtgc gcttcttcgc tggtggcgat gggcttgccg
     2341 acgagcaggc gcttgaaggg cgaggtcatg agctgacccc tttccggatc gggccgtggc
     2401 ccggagagga atcaggttgt gaggtcggcc ggagcggcgg gccccgcgtc ggctcccccg
     2461 gcgaccttgc ggtcagcgtg gaacggcacg gaggtgacga ccgtgttcgg gcggttccgc
     2521 aggcggtttc gcagcaccag cgcactctgg ttgtgcagga gctgctgcca ccacttgtcg
     2581 agcgtgaact cggggacgat caccgtcagg aagtcgtcgt catggcggtc gtcgagctcg
     2641 tcgatgtagg tcatgatcgg ccgtgacagc tcccggtacg gcgagtagag cgtctggagc
     2701 tcgaccggga tctggtggcg ctcccaggcc tcggtgatgc ggtcctgctc ctccgggttg
     2761 gacaccaccg acacggccac gaggcggtcg gggtgcagcg accgggcgta ggccagcgcg
     2821 atgagcgagc cgcggctgac ccgggcgacg aggacgatga cggtgttcgt cttgcgacgc
     2881 accttctcgt cgggctcgat gacgagcagc tcgtccatcg aacggtagtg ccggttgatg
     2941 ccctgcagca gcaccaccac cgccgggatc accagcaccg ggatccaggc accctcggtg
     3001 aacttcgaga tgatcaccac catcagcacg gtgaaggtca tggcgcagcc cagcgcgttg
     3061 acggccaggc cgcccttcca cttcggctcg cgcaagcgga ggtggtggcg gcacatgccg
     3121 aactgcgaca gcgtgaaccc gacgaacacc cccacggcgt agagcgggat cagggcactg
     3181 acctgggcct ggaaggccac gatgagcaca ccggccatgc ccgagaggat gaggacgccg
     3241 ttagagaaca ccagccggtc accgcggttg gcgagctgcc ggggcaggta gccgtcgcgc
     3301 gcgatgttgg acgagagctg ggggaagccg ttgtacgccg tgttcgccgc caggatgagg
     3361 atggcgaagg tggcgaactg gagcgcgtag tagaggaccg tccccgagcc gaagatgtga
     3421 ccgccgagct gcgagagcac cgtgtcgccg tcctcgacga agaccgggtg gaggtgactg
     3481 gccacgtagc tgacgccgag ggtgagcgtg ccgaggatga ccgccatcat gacgagcgtc
     3541 tgcgccgcgt tcttcgaccg gggcttgcgg aacgacggca cgccgttcga catggcctcg
     3601 acgccggcca gcacgacggc acccgaggag aacgcccgca gcaggaggat caggccgacc
     3661 gagccggtga gcgtcttcag gtccgccccg tgagccgtgg cgaagtcgtg cgccagcttc
     3721 tcgttgcccg gaatcgtgcc caggtgctgg aagtggatct tgtacatgcc gtaggcgatc
     3781 aggccgccga gcagcacggt gtaggcgtag gtcgggacgg cgaagatctt cccggcctcc
     3841 ttgaggccgc gcaggttgcc caccgcgagg agggccacga agaagagggc gagcccgacg
     3901 cgcagggtgg cgttgtcgtt gaagtggaac gcggagccga tggcgagggt gccggccgag
     3961 accgacaccg ccacggtcag cgtgtagtcc acgagcagcg acgcgccggc caccaggccc
     4021 gcggtctggc tgatgttgtc gcggctcacg atgtaagcgc cgccgccgtc ggggtaggcg
     4081 tagatcgtct ggcggtacga cgtggacacg accgccagca ggatcacggc g
//
LOCUS       DATMFS010000023         4788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_186853, whole genome shotgun sequence.
ACCESSION   DATMFS010000023 DATMFS010000000
VERSION     DATMFS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186853"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VNQ33_00425"
     CDS             <1..1284
                     /locus_tag="VNQ33_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ96587.1"
                     /translation="DRMLLSAGASTELVVAHGAALRLGLVVLPANTAYRERELAHLVR
                     DAAPSLAVVDDAERGRWVEAAAEAPIVITGPDVALPDGPDPAWLDGVGPDDPALLAYT
                     SGTTGAPKGALLTHANLLSSVEALGLAWRWTPEDRLVLPLPLFHVHGLGAALHGALTA
                     GGSVVLRRSFDAVDTLDAVAAHRGSLVFGVPTMWSRLAATGRLGELAALRLGVSGSAP
                     LDPGLHAAVAAETGHALLERYGMTETVILTSNPYDGDRRAGTVGRAIPGVGLRLAEAT
                     GEIEVRGPNVFSGYWQRPDANAASFRDGWFATGDVGSFDGDGYLRIVGRTKELIISGG
                     SNVYPREVEDVLRTHPAVHDAAVVGVPHPEWGEQVVAAVEGSGSSADVEVLLALARSE
                     LAPYKRPKRIVFVEALPRNALGKVVKADVAALLAD"
     gene            complement(1361..1789)
                     /locus_tag="VNQ33_00430"
     CDS             complement(1361..1789)
                     /locus_tag="VNQ33_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWJ96588.1"
                     /translation="MATALSPSEALDWVFAQARTAKIGVVTKSGAPLVAPIWVARDGG
                     ALVFNTGDGTAKGRAIRRDPRVSLCFDDETPPFSFVTIAGVAEIIEDLAEVRRWAAVI
                     GGRYMGEDQAEAYGERNGVPGELLIRVTPTKTTAALDIAD"
     gene            1830..2294
                     /locus_tag="VNQ33_00435"
     CDS             1830..2294
                     /locus_tag="VNQ33_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ96589.1"
                     /translation="MTAPVLLCADGSHHSTAALAAGVALLDPATPLVIVTVMAEPDPT
                     LVTGSGMAGGVMSPEAFDELEVEAGTDATAIATQAQRELNLPSADIKVLRGDPAASIC
                     MLADEVGAGAVVIGSRGHGGLKRAVLGSVSDHVVRNAPCSVIVTGPKGGAED"
     gene            complement(2305..3912)
                     /locus_tag="VNQ33_00440"
     CDS             complement(2305..3912)
                     /locus_tag="VNQ33_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96590.1"
                     /translation="MSRSPEAAIARRSLKQLWLGAVIWSVAFGGTVAASAVSYVSTFP
                     TEAERQQVAAATSGDAGLSVLLGPTTAIDTVGGYTVYKCFVFLTTIGAIWGLLAATRL
                     LRGEEDAGRWQLVLSGSTRAGRATAATLLALLAGVGILFVGTTVLTVAAGRDPDVGFG
                     IAESVLYGLSIVIAPAVFVMVGAVTSQLCRTRRMASSWAMGVFGACFLVRMVADAGPD
                     THWLRWATPFGWTELIEPFTGNDAWPLLPAIVAVVVLAVAAVALSARRDAGSSVFASN
                     DVTELRPFGLASPLGLAARLDLPILIAWCLGALVTGLSFGLIAKIATGAVPESLSDTL
                     DRFGAGGSFVEQYFGVAFLLAATVVALIAAGQLGAASDEESTGRLVHLLAGPVRRATW
                     FGGRLGLAAAGIVVAGLLAGVGAWAGAVTQGVDLGFGPLVGAGLNVVPTALLVLGIGA
                     VVLALAPRAAAVTVYVVVIGSLLVDLLASMVDGLAWTARISVFHSMALAPASSPDPGT
                     LAVTTVLGVGLCALATLLFVRRDVALG"
     gene            complement(3909..>4788)
                     /locus_tag="VNQ33_00445"
     CDS             complement(3909..>4788)
                     /locus_tag="VNQ33_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ96591.1"
                     /translation="IEIEQLTKRYGSVAAIDGLDLTIDEGEVFGYLGPNGAGKSTTIA
                     ILLGLIRATSGSARIFGQDVWREAPALHRRMASVPSEANLWPALTGEEALRFLGNVHG
                     SVDEAYRTELIERFQLVTDKRIRAYSHGNRQKVLLIAAFASRPDLLILDEPTTGLDPL
                     MEQVFQAAVREAAANGQTVLLCSHILSEVEAVCDRVAMLREGHLIETGHLDVLRGLVA
                     LRVHAAFDGSVPDLSGVDGVTHVAVDASSVECEVTGSVEPLLRALADGGAVRLTTREP
                     SLEELFVSHYGAGSEA"
BASE COUNT          682 a         1784 c         1742 g          580 t
ORIGIN      
        1 gaccggatgc tgctgtcggc cggcgcgtcg accgagctcg tcgtcgccca cggcgccgcc
       61 ctgcgcctgg gcctcgtcgt gctcccggcc aacaccgcct accgcgagcg ggagctggcc
      121 cacctggtgc gcgacgcggc cccgtcgctc gccgtcgtcg acgacgcgga gcggggccgg
      181 tgggtcgaag cggccgcgga ggcgccgatc gtgatcaccg gccccgacgt cgccctcccc
      241 gatggcccgg acccggcctg gctcgacggc gtcggccccg acgatccggc gctcctcgcc
      301 tacacctcgg gcaccaccgg cgcaccgaag ggcgcgctgc tcacccacgc caacctgctc
      361 tcgtcggtcg aggcgctggg gctggcctgg cgctggacac ccgaggaccg cctcgtcctg
      421 cccctccccc tgttccacgt gcacggcttg ggcgccgccc tccacggggc cctcaccgcg
      481 ggcggcagcg tcgtcctccg ccggtcgttc gatgccgtcg acaccctcga cgccgtggca
      541 gcccaccggg gatccctcgt gttcggcgtc ccgacgatgt ggagccgctt ggccgcgacc
      601 gggcgcctcg gtgagctggc ggcgctgcgc ctcggcgtgt cgggctccgc cccgctcgac
      661 cccgggctgc acgccgccgt cgccgccgag accggccacg cgctcctgga gcgctacggc
      721 atgaccgaga cggtgatcct cacctccaac ccctacgacg gcgaccgccg cgccggcacc
      781 gtcggcaggg cgatcccggg cgtgggcctg cgcctcgccg aggcgaccgg cgagatcgag
      841 gtgcgcggcc cgaacgtgtt ctcgggctac tggcagcggc ccgatgcgaa cgccgccagc
      901 ttccgcgacg gctggttcgc caccggcgac gtcggctcct tcgacggcga cgggtacctc
      961 cgcatcgtcg gccgcacgaa ggagctgatc atctccggcg gcagcaacgt ctatccccgc
     1021 gaggtggaag acgtcttgcg cacccacccg gcggtccacg acgccgcggt cgtcggcgtg
     1081 ccccatcccg agtggggcga gcaggtcgtc gccgccgtcg agggctcggg cagcagtgcc
     1141 gacgtcgagg tcctcctggc gctcgcccgg tcggagctgg cgccctacaa gcgacccaag
     1201 cgcatcgtgt tcgtggaggc cctcccccgc aacgcgctcg gcaaggtcgt caaggccgac
     1261 gtggccgcgc tcctggcgga ctgaggcccc gcaaccgcgc gaccccgcaa cccgcggcct
     1321 cccgaccgat cccgaacggg accggaagcc gagtcgccgg tcagtcggcg atgtcgagcg
     1381 cggcggtggt cttggtgggg gtgacgcgga tgaggagctc gccggggacg ccgttgcgct
     1441 cgccgtacgc ctcggcctgg tcctcgccca tgtagcggcc gccgatgacg gccgcccagc
     1501 gtcggacctc ggccaggtcc tcgatgatct cggccacccc ggcgatggtc acgaacgaga
     1561 agggcggtgt ctcgtcgtcg aagcagagcg acacccgcgg gtcgcggcgg atggcccggc
     1621 ccttcgcggt gccgtcaccc gtgttgaaca ccagggcgcc gccgtcgcgg gcgacccaga
     1681 tgggagcgac gagcggggcg ccggacttgg tgacgacgcc gatcttcgcg gtgcgggcct
     1741 gggcgaagac ccagtcgagt gcctcggagg ggctgagtgc ggtggccatg ggaggagtct
     1801 gccagcggat ggcgcggtac cgttcggcca tgactgctcc cgtgctcttg tgcgctgacg
     1861 gctcccacca ctcgacggcg gcgctggcag ccggcgtcgc cctcttggac ccggcgaccc
     1921 cgctcgtgat cgtcaccgtg atggcggagc cggatcccac gctggtcacc ggctcgggca
     1981 tggccggcgg cgtgatgtcg ccggaggcgt tcgacgagct cgaggtcgag gccggcaccg
     2041 acgccaccgc catcgccacg caggcccagc gggagctgaa cctgcccagc gccgacatca
     2101 aggtgctccg aggcgatccg gccgcctcca tctgcatgct ggcggacgag gtcggcgccg
     2161 gcgccgtcgt catcgggtcc cgaggccacg gcgggctcaa gcgggccgtg ctcggctccg
     2221 tgtcggacca cgtcgtgcgc aacgcgccgt gctcggtgat cgtcaccggc cccaagggcg
     2281 gcgcggagga ctgacggtcg gcggtcagcc gagagcgacg tccctccgga cgaagaggag
     2341 ggtggcgagg gcgcagaggc ccacacccag gacggtcgtg acggcgaggg tccccggatc
     2401 cggcgagctg gcgggcgcga gggccatgga gtggaagacg gagatgcggg cggtccatgc
     2461 cagcccgtcg accatcgagg cgagcaggtc gacgagcagc gagccgatga cgacgacgta
     2521 gacggtgacg gccgcggccc tcggcgcgag ggcgaggacc acggccccga tccccagcac
     2581 gagcagcgcc gtgggcacca cgttgagccc ggcgccgacg agcgggccga agccgaggtc
     2641 gaccccctgg gtcacggcac ccgcccaggc gccgacgccg gccaggagcc cggcgaccac
     2701 gatgccggcc gcggcgagac cgaggcggcc gccgaaccac gtcgcccgcc gcaccggtcc
     2761 ggcgaggagg tggaccaggc ggcccgtcga ctcctcgtcc gacgcggcac ccagctggcc
     2821 ggcggcgatg agggcgacga ccgtggccgc cagcaagaag gcgacgccga agtactgctc
     2881 cacgaagctc ccgccggccc cgaaccggtc gagcgtgtcg ctgagcgact ccgggaccgc
     2941 accggtggcg atcttggcga tgaggccgaa cgacaggccg gtcaccaggg caccgaggca
     3001 ccacgcgatc aggatcggca ggtccagccg ggcggcgaga ccgagcggcg acgccaagcc
     3061 gaagggccgc agttcggtca cgtcgttcga ggcgaacacc gagctgccgg cgtcgcgccg
     3121 agccgacagc gccacggcag cgacggccag cacgacgacc gccacgatcg cgggcagcag
     3181 cggccaggcg tcgttcccgg tgaacggctc gatgagctcg gtccacccga acggcgtcgc
     3241 ccaccgcagc cagtgcgtgt cggggccggc gtcagccacc atccggacga ggaagcaggc
     3301 accgaacacc cccatcgccc acgagctggc catccgacgg gtccggcaca gctgcgacgt
     3361 caccgccccg accatcacga acacggcggg cgcgatcacg atgctcaacc cgtagagcac
     3421 cgactcggcg atcccgaagc cgacgtcggg gtctcggccg gcggcgaccg tcagcaccgt
     3481 ggtcccgacg aacaagatcc cgacgccagc gaggagcgcg aggagcgtcg cggcggtggc
     3541 tcgacccgcc cgggtgctgc ccgacaagac gagctgccag cgcccggcgt cctcctcgcc
     3601 gcgcagcagc ctcgtggcgg ccagcaggcc ccagatggcg ccgatggtcg tgaggaagac
     3661 gaagcacttg tagaccgtgt acccgccgac cgtgtcgatg gccgtggtgg gtccgaggag
     3721 gacggacagg ccggcgtcgc cgctggtggc ggcggccacc tgctggcgct cggcttcggt
     3781 cgggaacgtc gacacgtagg acacggccga ggccgccacc gtcccgccga acgcgacgga
     3841 ccagatcacg gcgcccagcc acagctgctt gagggaccgc cgggcgatcg cggcctcggg
     3901 cgagcggctc acgcctcgct gcccgcgccg tagtgcgaga cgaacagctc ctcgagcgac
     3961 ggttcccgcg tggtcaggcg cacggcgcca ccgtcggcga gcgcccgcag cagcggctcg
     4021 accgacccgg tgacctcgca ctcgacgctg ctggcgtcga ccgccacgtg ggtcaccccg
     4081 tccaccccgc tcaggtccgg caccgacccg tcgaacgcag cgtggacccg cagggcgacc
     4141 agtccccgga gcacatcgag gtggccggtc tcgatcaggt gcccctcgcg gagcatggcg
     4201 acgcggtcgc agacggcctc gacctcgctc aagatgtgcg agcacagcag gacggtctgg
     4261 ccgttggccg ccgcctcgcg caccgccgcc tggaagacct gctccatcag cgggtcgagg
     4321 ccggtggtcg gctcgtcgag gatcagcagg tcgggccggc tggcgaaggc ggcgatgagc
     4381 aggaccttct ggcggttgcc gtggctgtag gcccggatcc tcttgtcggt gacgagctgg
     4441 aaccgctcga tgagctcggt gcggtaggcc tcgtcgacgc tgccgtggac gttgcccagg
     4501 aaccgcaggg cctcctcgcc ggtgagcgcc ggccagaggt tcgcctcgct cggcaccgag
     4561 gccatccgcc ggtggagcgc cggggcctcc cgccacacgt cctggccgaa gatgcgggcg
     4621 gaccccgacg tggcccggat caagccgagc aggatggcga tggtggtcga cttgcccgcg
     4681 ccgttgggcc cgaggtagcc gaacacctcg ccctcgtcga tcgtgaggtc gagcccgtcg
     4741 atcgcggcga cgctcccgta ccgcttggtg agctgctcga tctcgatc
//
LOCUS       DATMFS010000024         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_24070, whole genome shotgun sequence.
ACCESSION   DATMFS010000024 DATMFS010000000
VERSION     DATMFS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24070"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VNQ33_00450"
     CDS             complement(<1..916)
                     /locus_tag="VNQ33_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96592.1"
                     /translation="MTDPSGLRPPAGPQDAPRDDDAPRRLGRRALLSGGLTLAAGAGA
                     GAAAGALVARDDPSHRLATGTVAGGGGPWRYVSPGASIQAAIDAGAAAIQLGAGTYPI
                     DAPLAPTPGCTIRGIGEQTVLTATAEVPTVIAIGAGRPVGGVTIADLVVDAASRADTG
                     IDLDIVGTGGNLRGEPDAVCRLDALWVLDARVDGIAYRGSDTQATITSRVRVRRAGRH
                     GYRIEAPDNTWIACEATTQGTGGAGFWVAGANLHFDACKAWYCRGVGWEVRGSRNSFT
                     GCESQDTASHGWRITTARNTLTSCSADTA"
     gene            1133..1522
                     /gene="queF"
                     /locus_tag="VNQ33_00455"
     CDS             1133..1522
                     /gene="queF"
                     /locus_tag="VNQ33_00455"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWJ96593.1"
                     /translation="MTDPAAADGLTILGSTVRHPVEHLETFPAPHGCTKVRFTCEEVT
                     SMCPVTEQPDLSTVVIEYAPHQRCIESKSLKLYLWSFRDAAVFAEQMAVDIAAEVHRT
                     AAPSWVEVHVSQRARGGIETETFARLP"
     gene            complement(1548..1910)
                     /gene="queD"
                     /locus_tag="VNQ33_00460"
     CDS             complement(1548..1910)
                     /gene="queD"
                     /locus_tag="VNQ33_00460"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWJ96594.1"
                     /translation="MRITKDFTFEAAHRLPKVPEGHKCARLHGHSFGVSVSVDGPVGA
                     ATGWVLDFADLSAAWSPLHDQLDHRYLNEVPGLENPTSEVLATWIWDRLVEHLPALSE
                     IEIRETCTSRCTYRGPGT"
     gene            complement(1910..>2128)
                     /locus_tag="VNQ33_00465"
     CDS             complement(1910..>2128)
                     /locus_tag="VNQ33_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96595.1"
                     /translation="DLVLTKGDELKLVFPQPGLAPGAVEHLGFDHLFLQPMDGPDRAA
                     NTEAAVAHCLAHPRWRLSVQTHKYLGIP"
BASE COUNT          260 a          738 c          823 g          307 t
ORIGIN      
        1 ccgcggtatc ggccgagcag ctcgtgagcg tgttgcgggc cgtggtgatg cgccagccgt
       61 gcgaggcggt gtcctgggac tcgcagccgg tgaagctgtt gcggctgccg cggacctccc
      121 agccgacgcc ccggcagtac caggccttgc aggcgtcgaa gtgcaggttg gcaccggcga
      181 cccagaagcc ggcgccaccg gtgccctggg tggtggcctc gcaggcgatc cacgtgttgt
      241 cgggcgcttc gatgcggtag ccgtggcggc cggcgcgccg gacccgcacg cggctcgtga
      301 tcgtcgcctg ggtgtcgctg ccgcggtagg cgatgccgtc gacccgcgcg tcgagcaccc
      361 agagggcgtc gaggcggcag accgcgtcgg gctcgccgcg caggttgccc ccggtgccga
      421 cgatgtcgag gtcgatgccg gtgtcggccc ggctcgcggc gtcgaccacc aggtccgcga
      481 tcgtcacgcc gcccaccggc ctgccggccc cgatcgcgat gaccgtgggc acctccgcgg
      541 tcgccgtgag caccgtctgc tcgccgatgc cgcggatcgt gcagcccggc gtgggcgcga
      601 gcggcgcgtc gatcgggtag gtgcccgccc cgagctggat cgctgccgcg cccgcgtcga
      661 tggccgcttg gatcgacgcg ccggggctga cgtagcgcca cggtccgccg ccgcctgcga
      721 cggtccccgt cgcgaggcgg tgcgacgggt cgtcgcgggc gacgagcgcg ccggccgcgg
      781 ccccggctcc ggcgcccgcg gcgagcgtca acccgccgct cagcagggcg cggcgcccga
      841 ggcgccgggg cgcatcgtcg tcgcggggag cgtcctgcgg tccggcgggc ggcctgagac
      901 ccgatgggtc ggtcacctgg cggaccggct cgccaggagc cgtccgggaa ccgtcacgcg
      961 cccgcgtcgc cgagtggact cacccatctg ggcggcctcg tctcgtcgtc tcgtcgacct
     1021 cgtggatctg gatcccgcgg caccccgtct cctcccatcc gtccgacgat gtccgcaccg
     1081 ggccacgata ggcccgccgg cggtcagcgg aggcggtcgg taggctcaga gggtgaccga
     1141 tcctgctgcg gccgacggcc tgacgatcct ggggtccacg gtgcgccacc cggtggagca
     1201 cctcgagacg ttcccggcgc cccacggttg caccaaggtg cggttcacgt gcgaggaggt
     1261 cacctcgatg tgcccggtga ccgagcagcc ggacctctcg accgtggtga tcgagtacgc
     1321 cccgcaccag cggtgcatcg agtcgaagtc gctgaagctc tacctgtggt cgtttcgcga
     1381 cgccgccgtg ttcgccgagc agatggcggt cgacatcgcc gccgaggtcc atcgcacggc
     1441 cgcgccgtcg tgggtcgagg tccacgtctc ccagcgagcc cggggcggca tcgagaccga
     1501 gaccttcgcc cgtctcccct agagcccgga accggtcggg cggaggctca ggtgccgggg
     1561 ccgcggtagg tgcagcggct ggtgcaggtc tcgcggatct cgatctcgct gagggcgggg
     1621 aggtgttcca cgaggcggtc ccagatccag gtggcgagga cctcgctggt ggggttctcg
     1681 aggcccggca cctcgttgag gtagcggtgg tcgagctggt cgtggagcgg cgaccaagcc
     1741 gccgacaggt cggcgaagtc gaggacccag ccggtggccg cgccgacggg accgtcgacc
     1801 gacacgctga cgccgaacga gtggccgtgc agccgcgcgc acttgtggcc ctcggggacc
     1861 ttgggcagcc ggtgggccgc ttcgaaggtg aagtccttgg tgatgcgcat caggggatcc
     1921 cgaggtactt gtgggtctgg acgctgagcc gccaccgggg atgggcgagg cagtgggcga
     1981 ccgcagcctc ggtgttggcg gcgcggtcgg ggccgtccat cggctgcagg aagaggtggt
     2041 cgaacccgag gtgctcgacg gcaccggggg ccaggcccgg ttgtgggaac acgagcttga
     2101 gctcgtcgcc cttcgtgagg acgaggtc
//
LOCUS       DATMFS010000025         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_210260, whole genome shotgun sequence.
ACCESSION   DATMFS010000025 DATMFS010000000
VERSION     DATMFS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210260"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VNQ33_00470"
     CDS             <1..1176
                     /locus_tag="VNQ33_00470"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HWJ96596.1"
                     /translation="SGSVPGSLLNDFSMSEHDGHLRVAVTAQGSRRGTWPMPIDDVAG
                     TFVEDESVAVPGTPTVTDGSPGAAVAEEPTRAAEPVAPDTAPETVTGTVPDTTVPDDP
                     ATTTTTSEVGATTTSTSTSTTTSTSTSTTTSTSTSTSTTTTPPAGTTIPGPGPDDPFN
                     KIVVFDTEGNLDVVGSTPWFGHPGETLQGIRFDGDTAYAVTFLQTDPFYVVDLADPRA
                     PKVAGEVELPGFSAYLHPVGGGKVVGFGPGASGRQEAKLFDVSNPAAPKVVDGLVLGD
                     DSPVVFDHHAFVSLGDGRFAVPVTSWSNVTADCAIPSDAGPGADIGCAPSSSGIESQV
                     VEIGVVGDQLEEVARLTVELPEQASRAIPTKGGWAVLAGQSIGTVSDGGTQGTTVTL"
     gene            complement(1304..2503)
                     /locus_tag="VNQ33_00475"
     CDS             complement(1304..2503)
                     /locus_tag="VNQ33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ96597.1"
                     /translation="MPDHPTRDDIDLIAGDFWGRNPHDSFTWMRANAPVYWDGHAWGI
                     TRYADLKAISKDPATFSNAQGIRADADPLQMMIDMDDPEHFRRRKLVNKGYTPRRVRE
                     REDEVRAACTDIIDAVCEQGECDFVTDIAAWLPLIMIGNDLGVRPEDRAQLLEWSDAM
                     LSALTGDPEALEPATLAFIGYTEYANGVIADRTADPKDDLMSVLCHAEVDGDRLDHQA
                     IIDESLLILIGGDETTRHVISGGAYQLLANRDQWDRLQADRGLLPSALEEMLRWVSPI
                     KNMNRTATTDVVVNGQQIREGDNVLLLYPSANRDEDVFEDPFRFDVGRTPNDHVAFGF
                     GTHFCLGNSLARLELRVMFEELLGRLPDLELVGGDEPGYRAANFVSGYEHMPVRFTPT
                     KPSGRTA"
BASE COUNT          357 a          898 c          910 g          393 t
ORIGIN      
        1 tcgggctcgg tcccggggtc gctgctcaac gacttctcga tgtccgagca cgacggccac
       61 ctgcgggtcg ctgtcaccgc ccagggctcc cggcggggga cctggcccat gccgatcgac
      121 gacgtggccg gcaccttcgt cgaggacgag agcgtggcgg tgcccggcac tccgacggtc
      181 acggacggct cgcccggtgc tgccgtcgcc gaggagccca cccgagcggc ggagccggtg
      241 gcgcccgaca ccgctcccga gacggtgacc ggcacggtgc ccgacaccac cgtccccgat
      301 gatccggcga cgaccacgac cacctcggag gtgggggcga cgaccacctc cacgtccacc
      361 agcaccacga cgagcacgtc gacctcgacc accaccagca cgtcgacgtc gacctcgacg
      421 accacgacgc cgccggccgg cacgacgatc cccggtcctg gcccggacga cccgttcaac
      481 aagatcgtcg tcttcgacac cgagggcaac ctcgacgtcg tcggatccac cccgtggttc
      541 ggccaccccg gcgagacctt gcagggcatc cgcttcgacg gtgacaccgc ctacgcggtc
      601 acgttcctcc agaccgatcc gttctacgtc gtggacctgg ccgatccgcg ggcgccgaag
      661 gtggccggtg aggtcgagct ccccggcttc agcgcctacc tccacccggt cggcggtggg
      721 aaggtcgtcg gtttcggccc cggggccagc ggccggcagg aggccaagct gttcgacgtg
      781 tcgaacccgg cagcgccgaa ggtcgtggac gggctcgtcc tcggtgacga ctccccggtc
      841 gtcttcgacc accacgcctt cgtcagcctc ggcgacggac ggttcgccgt cccggtcacc
      901 agctggtcga acgtgaccgc cgactgcgcc atcccatccg atgccggccc gggggccgac
      961 atcgggtgcg ctccgtcgtc gtccgggatc gagagccagg tcgtcgagat cggcgtggtc
     1021 ggcgaccagc tcgaggaggt cgcccggctc accgtcgagc tgcccgagca ggcgtcccgg
     1081 gcgatcccca cgaagggcgg gtgggccgtc ctcgccggcc agtcgatcgg caccgtctcc
     1141 gacggcggga cccagggcac gaccgtcacg ctctgaccgg ctcggagcca tctgggacga
     1201 ccctgcggtc ctggtcgcat cgcctccgtc accggtcgac cggtgcggcg ttcgtcgtcg
     1261 aagccacgcg accggggcct cgagcgcccc gagccggagg gcgtcaggcg gtgcggccgg
     1321 acggcttcgt gggggtgaac cgcaccggca tgtgctcgta gccgctcacg aagttggcag
     1381 cccggtagcc gggctcgtcg ccgccgacca gctccaggtc gggcaggcgg ccgaggagct
     1441 cctcgaacat gacccgcagc tccagccggg cgaggctgtt gcccaggcag aagtgggtgc
     1501 cgaacccgaa ggccacgtgg tcgttcggcg tgcggcccac gtcgaagcgg aacgggtcct
     1561 cgaacacgtc ctcgtcgcgg ttggccgagg ggtagagcag caagacgttg tcgccctcgc
     1621 ggatctgctg gccgttgacc accacgtcgg tggtggcggt gcggttcatg ttcttgatgg
     1681 gactgaccca gcgcagcatc tcttcgagcg cggagggcag cagcccgcgg tccgcctgga
     1741 gccggtccca ctggtcgcgg ttggcgagca gctggtaggc accgccggag atgacgtggc
     1801 gggtggtctc gtcgccgccg atgaggatga gcagcgactc gtcgatgatc gcctggtggt
     1861 cgaggcggtc gccgtcgacc tcggcgtggc acaggacgct catcagatcg tccttcgggt
     1921 cggccgtgcg gtcggcgatc acgccgttgg cgtactccgt gtacccgatg aaggcgagcg
     1981 tcgccggctc cagtgcctcg gggtcgccgg tgagcgccga gagcatggcg tcgctccact
     2041 ccaggagctg cgcccggtcc tcgggccgca cgccgaggtc gttgccgatc atgatcagcg
     2101 gcagccaggc ggcgatgtcg gtgacgaagt cgcactcgcc ctgctcgcac acggcatcga
     2161 tgatgtcggt gcacgcggcg cgcacctcgt cctcccgctc gcggacccgg cggggcgtgt
     2221 agcccttgtt gaccagcttg cggcgtcgga agtgctccgg atcgtccatg tcgatcatca
     2281 tctggagcgg gtcggcgtcg gcccggatgc cctgggcgtt ggagaacgtg gccgggtcct
     2341 tggagatcgc cttcaggtcg gcgtagcggg tgatgcccca cgcgtggccg tcccagtaga
     2401 cgggtgcgtt cgcccgcatc cacgtgaacg agtcgtgggg gttgcggccc cagaagtcac
     2461 cggcgatgag gtcgatgtcg tcgcgggtcg ggtggtcggg cacgcatcgc ctccaagatc
     2521 tgacgggtcg tcatattctg gctgctgtga cggtagcg
//
LOCUS       DATMFS010000026         8244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_326247, whole genome shotgun sequence.
ACCESSION   DATMFS010000026 DATMFS010000000
VERSION     DATMFS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326247"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VNQ33_00480"
     CDS             complement(<1..1121)
                     /locus_tag="VNQ33_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWJ96598.1"
                     /translation="MREGWQLIRSQLWTQRRGIAFGAFVALAWTAAKVSVPALVALAI
                     DEGITPKDKGAIDHYALLIALAGVFVATFTGLRRYSAFREARKTEAVLRDRLFAHLQR
                     LHFAFHDGQQTGQLMSRANNDLQQIQNAVVLVPLTIANLATVGAVLVILVLKDPVLTL
                     LALGSLPAVNVLAKQFATKLQPTMTAVQQESAELSAVVEETVAGIRVVKGFAAEDTQS
                     ARLAAEADDLYDVSMEAARIRSVYWPALEVIPSIGLIMVLAYGGHQVLDGNLALGDLV
                     AFNAYVVLLVWPLRMLGWIVAMSQRAAASAQRVHEILATEPLVVDPPDPTPLPAAGGD
                     VRFDTVTFHYAGNSHAVLDGFDLHIPAGQSVALVGATASG"
     gene            1369..3717
                     /locus_tag="VNQ33_00485"
     CDS             1369..3717
                     /locus_tag="VNQ33_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96599.1"
                     /translation="MRLEQLVLFGPSDSFSVQFAPRVTVLAGLAADERDGMLATLLDA
                     MAGRLPNASVIFVDQAGRRVFADRMGATYADTGTAAPSLDQLLGTDPEVISELMTLRR
                     SDLAVGDQRSEEAITADLDDARSALEQLAAETAEATTYVVRVDAMRAELEQLDEVIAL
                     APEASARWEWIGQRNQLDELRAELAALDQPGDEDEAAADARLLAAVEELREAGEDWAE
                     ASTAATELGEALGPLPPVSDADLARVARTPDDLPAGFDARIAAVRATAEITVACEAAH
                     ATAVEEPADPGDGIVYQLAQLDQEALWAAHDAAVEARIAYEAELAEREDETDPDAESG
                     IEAAHHEVVRCQREVDRRFHAGILGSSLLTVGALLAGRSISVLVGVPVLAAAVALGYW
                     LLAIPRRNLAAAEREEETALGQADAGSWLGLHLRRIDDVMQPSDRRGLDSAVHRQAST
                     RLDWEELTGGTSLDAAGERRAAIVAHAAATDPKARAARERAAADALDAAREQARDARR
                     ELVEHLDGYGLSNEGATDLDPSQIRAVLEQRAAAGRFARKAIALREHQVQAASAGIRL
                     DHLLCRLGFDDGDDLAGRLERAIVSVEAARRRRHAAEDTRARSDLEGEIAELSALVSR
                     HRRLSWDLTPDPTEAPQDPAVLMDRRRALAEEVASLRGPDLGDVQRRSGLAAERVRAL
                     ESELVALTEGPTALRRRVADRIARTTFVGDAEEALPLVIDDALVDIDPSELFKVLDLV
                     VRLSSRTQIVLLTSDVTIARWARREAAHGIVTLLETDGAAIR"
     gene            complement(3851..5041)
                     /locus_tag="VNQ33_00490"
     CDS             complement(3851..5041)
                     /locus_tag="VNQ33_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96600.1"
                     /translation="MTTKSGGPAQSGQAVPLAEPAAGRLREPVFRPVIAASDTELLVA
                     GDDEPNGSGALRVRDTVVLVDLHDGNQREVAAPEADRPIAIVAAAATNDGFVIVGTRC
                     DQGDATSAEEPDCTPGTPVSALLADDGTWRDLPFPEALSPPDFAGIWSFDARLIPMPD
                     GSLGAVVSSHQAVAGSASPQVLMRLDPGTGWTEVGSMRDGGIDQACGGGKGQVYVLQR
                     TASDAPTDQPLAPVSLRLQRMSLSGGGPQDVPLPTVDASIGGVGVKIGCDADHVYVTS
                     SAPSTGASIEVFQSTDKGWGSVDYPGIGALPTSIISAASGLAITTSEMEPGRDIRTSV
                     TRILSDGRVRDLDGAASASLVVPQLGGDALVVVGPFSRLDGRQTGEGQASEARASVTI
                     EKFR"
     gene            complement(5284..5802)
                     /locus_tag="VNQ33_00495"
     CDS             complement(5284..5802)
                     /locus_tag="VNQ33_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWJ96601.1"
                     /translation="MAVYALGHQVPQIHPDAYISPEAVVIGSVAIGSESSVWPGAVLR
                     GDDGEIHIGARTSIQDGAIIHTTHFQPTTVGDECVIGHLAHLEGCTIGDGALVGSGSV
                     VLHQAVVETGALVGAGAVVSGGTVVPSRAMALGIPAKIRLDAVTPEMISLGMQSYVDR
                     GHRYRAELRRLD"
     gene            5843..7966
                     /locus_tag="VNQ33_00500"
     CDS             5843..7966
                     /locus_tag="VNQ33_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWJ96602.1"
                     /translation="MTEPSATDPADVTPPAAPPRPSPRPVERTFHGDTVIDPYAWLLD
                     ATDPETLPHLKAENAWTEASTAGQGALREAIFSEIKDRTLETDLSVPVRDGAWWYLTR
                     TEEGKAYPIHCRRPDDGTTTAWVADPAAEQVVLDPNELAGEGEYLGLGVLDVSPDGNW
                     LAYAIDREGDERHALRFRDLRDGQEPAETIDAVSYGFAWAADSRTCWYTLDDDAHRPN
                     RVVRHEVGTDPSRDTEVFRDDDERFHVSVGASRSGDVVVISAGSAITSESWLLDAHEP
                     TLDPVVVVAREQGVEYSVAHHPTGLYVTSNHGGAEDFALWRAPLDGIATAPRDGWEAV
                     LPHRPGTRLQGVETFAGHVIVHGRTDGLTAIWLLDPATGATTLFDTDDAVGTISPGAN
                     PTFGSTTYRFSYQSLTTPPSVLEQDIATGDRTVLKQLPVLGDFDPARYASAREWATAA
                     DGTEVPISLVWRPDAVPADGPAPCLLYGYGAYEMSMDPWFSIARLSLLDRGVVFAIAH
                     VRGGGELGRHWYEDGKFGAKHHSFSDFVACAEHLVATGRTTPSALAARGGSAGGLLMG
                     AAANLAPSAFRAVVAEVPFVDPLTTMLDPSLPLTVIEWEEWGDPLHTAEAYEWMKAYA
                     PYENVAPATEASYPAILATAGLNDPRVGFHEPAKWVARLRDQGHEALFKVELGAGHGG
                     PTGRYDAWRDEAFVLAWLLTALDVV"
BASE COUNT         1213 a         3112 c         2827 g         1092 t
ORIGIN      
        1 ccgctggccg tggccccgac gagggcgacc gactgcccgg cggggatgtg caggtcgaac
       61 ccgtcgagca cggcatgcga gttgccggcg tagtggaagg tcacggtgtc gaagcggacg
      121 tcaccgcccg ccgccgggag cggggtgggg tccggcgggt cgaccaccag cggctcggtg
      181 gccaagatct cgtgcacccg ctgcgccgag gccgccgccc gctgcgacat ggcgacgatc
      241 caccccagca tgcgcagcgg ccacacgagc agcacgacgt aggcgttgaa cgccacgagg
      301 tcgccgagcg ccaggttgcc gtcgagcacc tggtgaccgc cgtaggccag caccatgatc
      361 agcccgatgc tcgggatgac ctcgagcgcc ggccagtaca cggagcggat ccgggccgcc
      421 tccatcgaca cgtcgtagag gtcgtcggcc tcggccgcca ggcgggccga ctgcgtgtcc
      481 tcggccgcga agcccttcac gacccggatg ccggcgacgg tctcctcgac caccgcggac
      541 agctcggcgg actcctgctg gaccgcggtc atcgtcggct ggagcttggt ggcgaactgc
      601 ttggccagca cgttcaccgc cggcagcgag ccgagcgcga gcaaggtgag caccgggtcc
      661 ttcaggacca ggatcaccag cacggcgccg accgtggcca ggttggcgat cgtgagcggg
      721 accaggacca cggcgttctg gatctgctgg aggtcgttgt tcgcccggct catgagctgg
      781 ccggtctgct ggccgtcgtg gaaggcgaag tgcagccgct ggaggtgggc gaagagccgg
      841 tcgcggagca cggcctcggt cttgcgggcc tcgcggaacg ccgaatagcg ccggaggccg
      901 gtgaaggtgg cgacgaacac gccggccagg gcgatcaaca gggcgtagtg gtcgatcgcg
      961 cccttgtctt tgggcgtgat cccctcgtcg atggccaggg ccaccagcgc cggcaccgac
     1021 accttcgccg ccgtccaggc caaggcgacg aacgccccga aggcgatccc ccgacgctgc
     1081 gtccagagct ggctgcggat cagctgccag ccctctcgca ccgcgctcgg ttcggatggg
     1141 gatcgtcgac tgatggctgt gctccagtac cttgaccgcc gggtcaaggg tagtggtggg
     1201 ccatatggct caaccgcatc gattctcacc cggtgcggca caaacgcgct caactcgtgg
     1261 cctcggaacg ccgatgcacc agaggtcagc acttcttgac caccggttcg gcgggcgccg
     1321 gtgcccatcc gcccccgtac gtcccggcaa cgggagaagg tcggccacgt gcgcctcgag
     1381 caactcgtcc tcttcggacc cagcgacagc ttctccgtcc agttcgcccc ccgggtgacg
     1441 gtgctggccg gcctcgccgc cgacgagcgc gacgggatgc tcgccacgct gctcgacgcc
     1501 atggccggac ggctcccgaa cgcctcggtg atcttcgtgg accaagcggg ccggcgggtg
     1561 ttcgccgacc gcatgggggc cacctacgcc gacaccggca cggcggcgcc gtcgctcgac
     1621 cagctgctcg gcaccgaccc cgaggtcatc agcgagctca tgacgctgcg gcgatcggac
     1681 ctcgccgtcg gtgaccagcg atcggaggag gcgatcaccg ccgacctcga cgacgcccga
     1741 tcggccctcg aacagctcgc cgccgagacg gccgaagcca ccacctacgt cgtccgcgtg
     1801 gatgcgatgc gagccgagct cgagcagctc gacgaggtga tcgccctggc gcccgaggcc
     1861 agcgcccgct gggagtggat cggccagcgc aaccagctcg acgagctccg ggccgagctg
     1921 gcggcgctgg accagcccgg cgacgaggac gaggccgcgg ccgatgcccg cctcctcgcc
     1981 gctgtcgaag agctgcgcga ggccggcgag gactgggccg aggccagcac ggcggccacc
     2041 gagctcggcg aggccctcgg cccgctgcca ccggtgagcg atgccgacct cgcccgggtc
     2101 gcgcgcacgc ccgacgacct cccggccggc ttcgacgctc ggatcgcggc cgtccgggcc
     2161 accgccgaga tcaccgtcgc gtgcgaggct gcgcacgcca ccgccgtcga ggagccggcc
     2221 gaccccggtg acggcatcgt ctaccagctc gcccagctcg accaggaggc gctctgggcg
     2281 gcccacgacg cggccgtcga ggcccggatc gcctacgagg ccgagctcgc cgagcgggag
     2341 gacgagaccg accccgacgc cgagtccggg atcgaggcgg cccaccacga ggtcgtccgc
     2401 tgccagcgcg aggtcgaccg ccggttccac gccggcatcc tcggctcgtc cctgctcacc
     2461 gtgggcgccc tgctggcggg ccggtccatc tcggtcctcg tcggtgtgcc ggtgctggcg
     2521 gccgccgtcg ccctcgggta ctggctcctg gccatccccc gccggaacct cgccgccgcc
     2581 gagcgcgagg aggagacggc cctcggccag gccgacgccg ggtcgtggct cggcctccac
     2641 ctccggcgca tcgacgacgt catgcagccg agcgaccgcc ggggcctcga ctccgcggtg
     2701 caccgccagg cgagcacccg cctcgactgg gaggagctga ccggcggcac gtccctcgat
     2761 gcagcgggcg agcgccgcgc cgccatcgtg gcccatgccg cggccaccga cccgaaggcc
     2821 cgcgccgccc gggagcgggc tgccgccgat gccctcgacg cggcacggga gcaagctcgc
     2881 gacgcccgcc gcgagctggt cgagcacctc gatggctacg gcctctccaa cgagggcgcc
     2941 acggacctcg acccctccca gatccgcgcc gtgctcgagc aacgggccgc ggccggccgc
     3001 ttcgcccgca aggcgatcgc cctgcgcgag caccaggtgc aggccgccag cgccggcatc
     3061 cggctcgacc acctcctctg ccgactgggc ttcgacgacg gcgacgacct cgccggccgc
     3121 ctcgaacggg cgatcgtctc ggtcgaggcg gcacgccggc gccgccacgc cgccgaggac
     3181 acccgcgccc gcagcgacct cgaaggcgag atcgccgagc tcagcgcgct ggtctcacgg
     3241 caccgccgcc tcagctggga cctgaccccc gatccgaccg aggcgcccca ggacccggcg
     3301 gtgctgatgg accgccgccg agccctcgcc gaggaggtgg cctcgttgcg cgggcccgat
     3361 ctcggtgacg tgcagcgccg ctccggactc gccgccgagc gcgtccgggc tctggaatcg
     3421 gagctcgttg cgctcaccga gggcccgaca gcgcttcgcc gccgggtcgc ggaccgcatc
     3481 gcccgcacca ccttcgtggg cgatgccgag gaggccctgc cgctcgtcat cgacgacgcc
     3541 ctcgtcgaca tcgacccgag cgagctgttc aaggtgctcg acctggtcgt ccggctctcc
     3601 agccggaccc agatcgtgct gctcaccagc gacgtcacca tcgcccggtg ggctcgccgc
     3661 gaagccgccc acggcatcgt gaccctcctc gagaccgacg gcgccgccat ccgctgaccc
     3721 gccgtcggca gacggcgccc ggcccccagg ccctcgacct cgccgccgga ccccggctga
     3781 cctgcctacc ggcatgcatg acagaaggat tcgcctgcgt cgagtcgcac gcggtcgggc
     3841 tgccgccgcg tcacctgaac ttctcgatgg tcacggatgc tcgagcctcc gaagcttgcc
     3901 cttcgccggt ctgtcggcca tcaagccgcg agaacggacc caccacgacg agggcatcac
     3961 cgccgagctg cggtacgacg agcgaagcag acgctgcccc atcgaggtct cggaccctcc
     4021 catccgaaag gatcctcgtc accgaagtac ggatgtcgcg gcccggttcc atctcgctcg
     4081 tggtgatcgc cagcccggat gccgcggaga tgatcgatgt cggcaacgcg ccaataccgg
     4141 gatagtcgac cgaaccccaa cccttgtccg tggactggaa gacctcgatg gaggcgccgg
     4201 tcgaaggggc ggatgacgtc acgtagacgt ggtctgcatc gcacccgatc ttcacgccca
     4261 ctccgccgat gctggcatcg acggtcggga gcggaacgtc ttggggtcca ccacccgaaa
     4321 gggacatgcg ctgcaaccgg agggacactg gggccagggg ttgatcggtc ggagcatcgc
     4381 tcgccgtgcg ctgcagcacg tacacctgcc ccttgcctcc gccgcaggct tggtcgattc
     4441 cgccatcacg catcgacccg acctccgtcc accctgtccc agggtcgagc cgcatcagga
     4501 cctggggcga tgcggacccg gcgaccgctt ggtgagagga gaccacggca ccgagcgagc
     4561 cgtccggcat cgggatgagc cgagcatcga acgaccagat gccggcgaag tccggcggac
     4621 tcaaggcctc ggggaacgga aggtctcgcc aggttccgtc gtcagcgagc agggcggaga
     4681 ccggagtacc cggtgtgcag tcgggttcct ccgccgaggt ggcatcgccc tgatcgcagc
     4741 gcgttccgac gatcacgaat ccatcgttcg tcgcagcagc ggcgacgatg gcgatgggac
     4801 gatccgcttc cggcgcggcg acctcgcgct ggttcccatc gtgcaggtcc accaggacca
     4861 cggtgtctcg aacccgcagc gctcctgacc cgttcggctc gtcgtcaccc gccacgagca
     4921 gctcggtatc cgaggcggcg atgaccggcc tgaagaccgg ctcacgcaag cggccggctg
     4981 ctggctcggc caagggcacg gcctggccgc tctgggccgg gccaccgctc ttggtcgtca
     5041 tggccgaatc cccggcgctg cagcccgcaa cgacgaggca gagcacggcg agcaggcaac
     5101 gaggtctcat ccgacgctgt cgcctggatc gccgcactgc ggaggagcgc ttcgcgcacg
     5161 ctggctgcga ccgctgcatg tcaaccgttc tcagatcagg aaatactgtt gtctcctgga
     5221 ctttacgcca gtttcctgca gcctgggtcc gaacctggtc ttcggaccgc gcccgcggca
     5281 cggtcagtcc aggcggcgga gctcggctcg gtagcggtgg ccgcggtcga cgtaggactg
     5341 catgccgagc gagatcatct cgggcgtgac ggcatcgagc cggatcttgg cggggatgcc
     5401 gagggccatc gcccgcgacg ggacgaccgt gcctcccgac accaccgccc ccgctccgac
     5461 cagtgccccc gtctcgacca ccgcctggtg cagcacgacc gagcccgacc cgaccagcgc
     5521 gccgtcaccg atggtgcagc cctcgaggtg ggcgaggtgg ccgatcacgc actcgtcgcc
     5581 caccgtcgtg ggctggaagt gggtggtgtg gatgatggcg ccgtcttgga tggaggtgcg
     5641 ggcgccgatg tggatctcgc cgtcgtcgcc ccgcagcacc gcacccggcc agaccgagct
     5701 ctcggagccg atggccaccg agccgatgac caccgcctcg ggcgagatgt aggcgtcggg
     5761 gtggatctgc gggacctggt gacccagggc gtacacggcc atccgcggag ggtacccggc
     5821 atctgcccgt agcctcgccg ctgtgaccga accctccgcg accgaccccg ccgacgtgac
     5881 cccacccgcc gctccgcccc gcccctcgcc gcgcccggtc gagcgcacgt tccacggcga
     5941 cacggtcatc gacccgtacg cttggctgct cgacgccacc gatcccgaga ccctccccca
     6001 cctgaaggcg gagaacgcgt ggaccgaggc gtcgaccgcc ggccaagggg cgctccggga
     6061 ggcgatcttc agcgagatca aggaccggac gctcgagacc gatctgtcgg tgcccgtgcg
     6121 cgacggcgcc tggtggtacc tgacgcgcac cgaggagggg aaggcctacc ccatccactg
     6181 ccgccggccc gacgacggca ccaccacggc ctgggtggct gacccggcgg ccgagcaggt
     6241 cgtcctcgac cccaacgagc tggccggcga gggcgagtac ctgggcctgg gtgtgctcga
     6301 cgtgagcccc gacggcaact ggctggccta cgccatcgac cgcgagggcg acgagcgcca
     6361 cgccctgcgg ttccgcgacc tgcgagacgg ccaggagccg gccgagacca tcgacgccgt
     6421 ctcctacggc ttcgcctggg ccgccgactc ccgcacctgc tggtacacgc tcgacgacga
     6481 cgcccaccgc ccgaaccggg tcgtccgcca cgaggtcggc accgatccga gccgcgacac
     6541 cgaggtcttc cgagacgacg acgagcgctt ccacgtgtcg gtcggggcgt ctcggtccgg
     6601 cgacgtcgtg gtgatcagcg ccggcagcgc catcaccagc gagagctggc tgctcgatgc
     6661 ccacgagccg acgctcgacc cggtggtggt cgtggcacgc gagcagggcg tggagtactc
     6721 ggtggcccac caccccaccg gcctctacgt gaccagcaac cacggcggcg ccgaggactt
     6781 cgccctgtgg cgcgccccgc tcgacggcat cgccaccgcc ccgcgcgacg ggtgggaggc
     6841 ggtcctcccc caccgccccg gcacccggct ccagggggtg gagaccttcg ccggccacgt
     6901 catcgtccac ggccgcaccg acggcctcac cgccatctgg ctgctcgacc cggccaccgg
     6961 ggcgacgacg ctgttcgaca ccgacgatgc cgtcggcacc atcagccccg gcgccaaccc
     7021 cacgttcggc tcgaccacct accggttcag ctaccagtcc ctcaccaccc cgccgtcggt
     7081 cctcgagcag gacatcgcca ccggcgaccg gacggtgctc aagcagctgc ccgtgctggg
     7141 cgacttcgat ccggctcggt acgcgtcggc ccgcgagtgg gccaccgccg ccgacgggac
     7201 ggaggtgccg atctcgctgg tgtggcgccc cgacgccgta cccgccgacg gaccggcccc
     7261 gtgcctgctc tacgggtacg gcgcctacga gatgagcatg gacccctggt tctcgatcgc
     7321 ccggctgtcg ctgctggacc ggggcgtcgt gttcgccatc gcccacgtcc gcggcggcgg
     7381 ggagctgggc cgccactggt acgaggacgg caagttcggc gccaagcacc actcgttctc
     7441 ggacttcgtc gcctgcgccg agcacctcgt cgccacgggg cgtacgacgc cttcggccct
     7501 ggccgcccgt ggcggttcag ctggcgggct cctcatgggc gcggccgcca acttggcccc
     7561 gtcggcgttc cgcgccgtcg tggccgaggt cccgttcgtc gacccgctca ccacgatgct
     7621 cgacccctcg ctcccgctca ccgtcatcga gtgggaggag tggggcgacc cgctccacac
     7681 cgccgaggcc tacgagtgga tgaaggccta tgccccctac gagaacgtgg ccccggcgac
     7741 ggaggcgtcc tacccggcga tcttggccac cgccggcttg aacgacccgc gggtcgggtt
     7801 ccacgagccg gccaagtggg tggcccgcct ccgagaccag ggccacgagg ccctgttcaa
     7861 ggtggagctg ggtgccggcc acggcggccc caccggccgc tacgacgcct ggcgcgacga
     7921 ggccttcgtc ctcgcctggc tcctcaccgc cctcgacgtg gtgtgaggcg gaccgggccg
     7981 ctcaccaacc gccgatctca ccgagcgtgc cggagacggc ggccccaggc gatttcgggt
     8041 cacctgggca cctcggaggt gcacagacga cccgaaagag ggggtgtggc ggcacgagcg
     8101 aggcggcctc catgttcaag ccaggtggct gtagccgcct cggaagaaga cgagggggtg
     8161 gccctccgcc gtgaccgcca ggtcgtggac ggcgcccacg acgatctcgt ggtcgccggc
     8221 gtcgtggagg tcctgggtct cgca
//
LOCUS       DATMFS010000027         7451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_187038, whole genome shotgun sequence.
ACCESSION   DATMFS010000027 DATMFS010000000
VERSION     DATMFS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187038"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VNQ33_00505"
     CDS             complement(<1..428)
                     /locus_tag="VNQ33_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ96603.1"
                     /translation="MIKLENVSKVFKGDVVALREADCEINKGEFVFLVGPSGSGKSTM
                     IRLLNKQEEPSSGSIFVAGKDIGELNSWKVPFLRRNIGCVFQDYKLLANKTVYENVAF
                     ALEVIGRPRHVIKTQVPAILELVGLAKKHKNYPDELSGGE"
     gene            complement(565..1674)
                     /gene="prfB"
                     /locus_tag="VNQ33_00510"
     CDS             complement(565..1674)
                     /gene="prfB"
                     /locus_tag="VNQ33_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWJ96604.1"
                     /translation="MRDVSDDISALRKRHDEAATYLKVAELRARQPQLETEASRPDLW
                     DDAEQARKITGELSSVNDDLERYDGLGERIDDVETLAELAREEGDDSVEAEIEGALRS
                     LAADFGDLELRSLFTGEHDELDVIAEIQAGEGGADSQDWANMLLRMYLRWAERKGLDT
                     EIENVSAGSEAGISSASFLVKGRYAYGLMRSEHGVHRLVRMSPFNAQGKRQTAFAALK
                     VTPFLEDVPDIVIEDKDLRVDTYRSSGAGGQHVNVTDSAVRLTHIPTGIVTTCQNQRS
                     QHQNKDKAMQYLKAKLAEVQREERQAELDAIRGVQHSVGFGSQIRSYVMQPYQMVKDL
                     RTEHETGNVDGVLDGNLDEFMEAYLRWQRSQALEA"
     gene            1757..2155
                     /locus_tag="VNQ33_00515"
     CDS             1757..2155
                     /locus_tag="VNQ33_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HWJ96605.1"
                     /translation="MTRSVPPPVLLFDGDCAFCSTCARWLAARIPMPVAVEPWQWTDL
                     EPLGVAVEEVDAAVVLVDVDLHRTHGPDALAGLLRSSTSGAWRACGRVLGLRPVRALA
                     WPTYRWISRNRHRMPGGTAQCSLPQAERHT"
     gene            2203..3957
                     /locus_tag="VNQ33_00520"
     CDS             2203..3957
                     /locus_tag="VNQ33_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ96606.1"
                     /translation="MPRPDSPAGSRLLLSYLRPERRPLSALLALLVVAMVLPLAGPLL
                     VGAFVDAALDGEPAARLAALGGLFLLATLTGDALQLVVTWFSVRLAWRVGNRLRVDLC
                     RHALGLDLDWHGGHSAGQLIERIDGDIDAVTRFASTAVLQLAGNAILVAGVLVVSAVI
                     DWRASVLIALTVAVAVAVLVRMRRVAVPYYDEEREVQSHLYGDLEERLGGLEDIRANG
                     AGPWAVQKLQEHSAGWWRTARRAAARGDGGLTLAGGVFAAGSVAVLGLSAWQCRQGAL
                     SVGSVLALLRFSQMVSDPLWRVAEQLAEAQKAVAGSRRAARLLAEESALPDGHRDDLP
                     AGPLAVAFDGASFGYGTGTDVLHDLTFSLPAGSSLGVVGRTGSGKTTIGRLVARLWDT
                     EAGSVSVGGIDVRALTQRGLRSRIGIVTQEVEVFRASVRDNLTVFGTLGASDEAVIDA
                     LGTVGLDDWLRSLDGGLDQHLEGDTELSAGEGQLLAFARLLLADPGIVILDEASSRLD
                     PHTEQRLLAATQQLLAGRTAIIIAHRLSTLDRVDEVLVLDHGRLAEHGPRAELAADPG
                     SRFHHLLATSRAAQEVGR"
     gene            3954..5675
                     /locus_tag="VNQ33_00525"
     CDS             3954..5675
                     /locus_tag="VNQ33_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ96607.1"
                     /translation="MSAPETVSSARFAWRILKADPWTWGISLALWVAFFSMPLPAGLV
                     LRAVVDQLPDGGGTPIVVLLAVLAGFEVGRWIILFPAIVQWHGAWVFWHTVPRVNALR
                     SLVSDPGPAAGRLPGSSGEAVSRFRDDARDVAQVLDVWLDLIAAGITTTAGVVVLYLI
                     SPVAATAIVAPILLVLGIGHLLAARLREWRWAERTATAGVTGFIGDAFGAIATVKVTA
                     AEPAVLDRFARLGSTRAVAARRDQVGTQLAQTLGGITANAGLGSALVLIAPAMSRGDL
                     STGDIALFTAYATAVASLPRITARWSTFQRQGDVSAARLGRLMPGHDPDQASAAVTTY
                     LRDGPPPFPVVVPPPPAERAGRDHLASLRAEDLRVRLDGTEQLDGVGLEIHRGQFVVI
                     TGPVGAGKSVLLRALLGLVPRVEGAVWWNGEPVVDPASFLVPPRAAYLPQIPRLFSES
                     LADTVLLGHDPAGLAAALEQARLVEDLADMPHGLGTTVGPKGVRLSGGQIQRTAAARA
                     LVRTPELLVVDDLSSALDVATEAEIWDGLFARDASTLTVLAVSHRPRVLERADQVIEL
                     RAGRRVR"
     gene            complement(5754..5960)
                     /locus_tag="VNQ33_00530"
     CDS             complement(5754..5960)
                     /locus_tag="VNQ33_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96608.1"
                     /translation="MSKTKSLYAWNWITGSNSNRPKRPSEARVTVAGTRRYLPWARAD
                     MGTPRPVLPWEHRLGNDWRGIKAS"
     gene            complement(6080..>7451)
                     /locus_tag="VNQ33_00535"
     CDS             complement(6080..>7451)
                     /locus_tag="VNQ33_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWJ96609.1"
                     /translation="RMLDKRGIAHEVLNAKQHTREAEIVTQAGRLHSITVATNMAGRG
                     VDILLGGNPEGLAIRDLRAEGVDMESEEGAERLKALTKQYKVETDAEGTKVRDLGGLY
                     VLGTERHDSRRIDNQLRGRSGRQGDPGESRFYLSLEDELMRLFATGAMNWVMGKALPD
                     DVPIEARMVTKAIERAQNTVEQRNAEIRKNVLKYDEVMNEQRKVIYKRRDQILEEADL
                     RSETLDYLEEAIDTSVATFCVSEATDEWDVEGLHMEIASFWPTGLTEEQLADADNTEE
                     LQELLLDDATAYYEKREEELGPDTMRQIERQVMLQIVDQHWREHLLEMDYLEEGINLR
                     AMGQKDPLVEWQREGFEMFGLMMTSIAQDFVKYVMHVQVVVDEPAADTPVTQMQTSGP
                     DDPSSAGNGMVEAARAQAAVDGTAVAVEEEVVNTPVVKSDWDKTPRNAPCPCGSGKKF
                     KQCHGA"
BASE COUNT          881 a         2780 c         2557 g         1233 t
ORIGIN      
        1 tgctcgccgc cggacagctc gtcggggtag ttcttgtgct tcttggccag gccgacgagc
       61 tccaagatgg cgggcacctg ggtcttgatg acgtgacggg gacggccgat gacctcgagg
      121 gcgaaggcga cgttctcgta caccgtcttg ttggcgagca gcttgtagtc ctggaagacg
      181 cagccgatgt tgcggcgcag gaacggcacc ttccagctgt tcagctcgcc gatgtccttg
      241 ccggccacga agatgctgcc gctgctcggc tcctcctgct tgttgagcag gcggatcatg
      301 gtgctcttgc ccgatccgga gggaccgacg aggaacacga actcgccctt gttgatctcg
      361 caatcggcct cccggagggc gacgacgtca cccttgaaga ccttcgagac gttttcgagc
      421 ttgatcattc cttggggacc ccagggtcgg tcggtgtgct tgctccctcg actggccgcg
      481 gccgagcacc tgtcgacgtt accgcagggg cctcgcggtt ccgcggcacc cgccccgcat
      541 cggcggctgg agcgggcgtc ctcctcaggc ttcgagcgcc tggctgcgct gccagcgcag
      601 gtaggcctcc atgaactcgt cgaggttgcc gtcgaggaca ccgtcgacgt tgccggtctc
      661 gtgctcggtc ctcaggtcct tgaccatctg gtacggctgc atcacgtacg agcggatctg
      721 cgagccgaac ccgaccgagt gctggacacc gcggatggcg tcgagctcgg cttggcgctc
      781 ctcgcgctgc acctcggcca gcttggcctt gaggtactgc atcgccttgt ccttgttctg
      841 gtgctggctg cgctggttct ggcaggtggt cacgatgccc gtggggatgt gggtgaggcg
      901 caccgccgaa tcggtcacgt tgacgtgctg gccgcccgcc cccgacgacc ggtaggtgtc
      961 gacccggagg tccttgtcct cgatgacgat gtcggggacg tcctcgagga acggggtgac
     1021 cttcagggcc gcgaaggcgg tctggcgctt gccctgggcg ttgaacgggc tcatgcggac
     1081 gaggcggtgg acgccgtgct ccgagcgcat cagcccgtag gcgtagcggc ccttgaccag
     1141 gaacgatgcc gatgagatgc cggcttcgga accggccgac acgttctcga tctcggtgtc
     1201 gaggcccttg cgctcggccc agcgcaggta catgcgcagc agcatgttgg cccagtcctg
     1261 cgagtcggcg ccgccctcgc cggcctggat ctcggcgatg acgtcgagct cgtcgtgctc
     1321 accggtgaag aggctgcgca gctccaggtc gccgaagtcg gccgccaggg accgcagcgc
     1381 tccctcgatc tcggcctcga ccgagtcgtc gccctcctcg cgcgccagct cggccagggt
     1441 ctcgacgtcg tcgatccgct caccgagccc gtcgtagcgc tccaggtcgt cgttcaccga
     1501 ggagagctcg ccggtgatct tgcgggcctg ctcggcgtcg tcccagaggt ccgggcgcga
     1561 ggcctcggtc tccagctggg gctggcgggc gcggagctcg gcgaccttga ggtaggtcgc
     1621 cgcctcgtcg tggcgcttgc gcagggcgct gatgtcgtcg ctgacgtctc gcatggtctc
     1681 ttgatcctgc ccgatcgccg atcgttccgg gcaaccggtg gtgggaaccg ctggtcgcag
     1741 gaccacactg gggaccatga cgaggtccgt gccgccacct gtgctcctgt tcgacggcga
     1801 ctgcgcgttc tgctccacct gcgcccgctg gctcgccgcc cgcatcccga tgccggtcgc
     1861 cgtcgagccc tggcagtgga ccgacctcga gcctctgggc gtcgccgtcg aggaggtcga
     1921 cgccgcggtc gtcctcgtcg acgtcgacct gcaccggacg cacggccccg atgccctcgc
     1981 cgggctgctg cgctcgagca cgtcgggtgc gtggcgggcg tgcgggcggg tgctgggcct
     2041 ccgcccggtg cgggccctgg cgtggccgac ctaccgctgg atctcccgca accgccaccg
     2101 gatgcccggc ggcacggccc agtgctcgct cccccaagcc gagcggcaca cctgacaaat
     2161 ccctgaagcg cgggaccacg gggtccgtag cgtggccgcg ctgtgcctcg tcccgactcc
     2221 cccgccggca gccgcctgct gctctcgtac ctgcgccctg agcgccggcc cctcagcgcg
     2281 ctcttggccc tgctggtcgt cgccatggtc ctgccgctcg ccggcccgct cctcgtcggc
     2341 gcgttcgtgg acgccgcgct cgacggcgaa ccggcagcgc ggctcgccgc gctgggcggg
     2401 ctgttcctcc tcgccaccct caccggcgac gcgctgcagc tcgtcgtcac ctggttctcc
     2461 gtgcggctcg cctggcgagt gggcaaccgg ctccgggtcg acctctgccg ccacgccctc
     2521 ggcctcgacc tcgactggca cgggggccac tccgccggac agctgatcga gcgcatcgac
     2581 ggcgacatcg acgccgtcac ccgcttcgcc tccaccgccg tcctccagct ggccggcaac
     2641 gccatcctcg tcgccggcgt gctcgtcgtc tccgccgtca tcgactggcg ggcgagcgtg
     2701 ctcatcgccc tcacggtcgc cgtcgccgtg gcggtcctcg tccggatgcg gcgggtcgcg
     2761 gtgccgtact acgacgagga gcgggaggtg cagtcgcacc tctacggcga cctcgaggag
     2821 cgcctcggcg gcctcgagga catccgggcg aacggcgccg gcccgtgggc ggtccagaag
     2881 ctccaggagc actccgccgg ctggtggcgg acggcgcggc gggccgcggc ccgcggcgac
     2941 ggcggcctga cgctcgccgg cggcgtgttc gccgccggct cggtcgcggt gctgggcctc
     3001 agcgcctggc agtgccggca gggcgcgctg agcgtgggct cggtgctggc cctcctccgg
     3061 ttctcccaga tggtgagcga tccgctctgg cgcgtggccg agcagttggc cgaggcccag
     3121 aaggcggtgg ccggcagccg tcgggccgcc cgcctcttgg ccgaggagtc cgcgctcccc
     3181 gacggccacc gtgacgacct ccccgccgga ccgctggcgg tggccttcga cggcgcctcg
     3241 ttcggctacg gcaccggcac cgatgtcctc cacgacctca cgttctccct ccccgccggg
     3301 tcctccctcg gcgtcgtcgg ccggaccggc agcggcaaga cgaccatcgg ccgcctcgtc
     3361 gcccgcctgt gggacaccga ggccggttcg gtgtcggtcg gcggcatcga cgtgcgcgcc
     3421 ctcacccagc ggggcctgcg gagccgcatc ggcatcgtca cccaggaggt cgaggtcttc
     3481 cgggcctcgg tccgcgacaa cctgaccgtc ttcggcaccc tcggcgccag cgacgaggcc
     3541 gtcatcgacg cgctggggac cgtcggactc gacgactggc tccgatcgct cgacggcggc
     3601 ctcgaccagc acctcgaggg cgataccgag ctctccgccg gcgaggggca gctcctggcg
     3661 ttcgcccggt tgctcctggc tgacccgggc atcgtgatcc tcgacgaggc ctcgagccgc
     3721 ctcgacccgc acaccgaaca acgcctcctc gccgcaaccc agcagctcct cgccgggcgc
     3781 accgcgatca tcatcgccca ccgcctctcg accctcgacc gggtcgacga ggtcctcgtg
     3841 ctcgaccacg gacgcctggc cgagcacggc ccccgagccg agctcgccgc cgaccccggc
     3901 agccgcttcc accacctgct cgccaccagc cgggccgccc aggaggtcgg acgatgagcg
     3961 cgccggagac ggtcagcagc gcccggttcg cctggcgcat cctcaaggca gacccgtgga
     4021 cgtggggcat ctcgctcgcg ctctgggtgg cgttcttctc catgcccctg cctgcgggcc
     4081 tggtgctgcg ggccgtcgtc gaccagcttc ccgacggcgg cggcacgccg atcgtcgtgc
     4141 tcctggcggt cctcgccggg ttcgaggtcg gacgctggat catcctgttc cccgcgatcg
     4201 tccagtggca cggcgcctgg gtgttctggc acaccgtccc ccgggtgaac gccctgcgct
     4261 cgctcgtatc ggacccggga ccggccgcgg gccggctccc cggctcgtcc ggcgaggccg
     4321 tgagccgctt ccgcgacgac gcccgcgacg tcgcccaggt gctcgacgtg tggctggacc
     4381 tgatcgccgc cggcatcacc acgacggccg gggtcgtcgt cttgtacctg atctcccccg
     4441 tggcggcgac ggcgatcgtg gccccgatcc tgctcgtgct cggcatcggc cacctcctcg
     4501 ccgcccggct ccgcgagtgg cggtgggccg agcgcaccgc caccgccggc gtcaccggct
     4561 tcatcggcga cgcgttcggc gccatcgcca ccgtcaaggt caccgcggcc gagccggcgg
     4621 tcctcgaccg cttcgcccgt ctcgggagca cccgcgccgt cgcggcgcgc cgcgaccagg
     4681 tcggcacgca gctggcccag acgctcggcg gcatcaccgc caacgccggg ctcggctcgg
     4741 cgctggtgct catcgccccc gccatgagcc ggggcgacct gtccaccggc gacatcgccc
     4801 tgttcaccgc ctacgccacc gcggtggcca gcctcccgcg catcaccgcc cggtggtcga
     4861 cgttccagcg ccaaggcgac gtctccgccg cccgcctcgg gcgcctcatg cccggtcacg
     4921 acccggacca ggcctccgcc gcggtcacca cctacctccg ggacggacct cccccgttcc
     4981 ccgtggtggt cccaccaccg cccgccgagc gcgccgggcg cgaccacctg gcatcgctgc
     5041 gcgccgagga tctccgggtc cggctcgacg gcaccgagca gctcgacggg gtcggcctgg
     5101 agatccaccg gggccagttc gtcgtgatca ccggccccgt cggcgccggc aagtccgtgc
     5161 tgctgcgcgc cctgctgggc ctggtgccgc gggtcgaggg agccgtctgg tggaacggcg
     5221 agccggtcgt cgacccggca tcgttcctgg tgccgccccg cgccgcctac ctgcctcaga
     5281 tcccgcgcct gttctccgag tcgctcgccg acacggtgct gctcggtcac gaccccgccg
     5341 gcctggctgc cgcgctcgag caggctcgcc tggtggagga cctcgccgac atgccccacg
     5401 gcctcggcac caccgtcggc ccgaagggcg tccgcctgtc cggcggccag atccagcgca
     5461 ccgccgccgc ccgagccctg gtccgcaccc cggagctgct cgtcgtcgac gacctctcga
     5521 gtgcgctcga cgtggccacc gaagccgaga tctgggacgg cctgttcgcc cgcgacgcgt
     5581 cgaccctcac cgtcctcgcg gtgagccacc ggccgagggt cctggaacgg gccgatcagg
     5641 tcatcgagct tcgggcgggc cgccgagtcc gctgacccgg cggccgcgcc cggtgctcca
     5701 gccaccgggc ccagctgctc cctttggggg atgagagcgc cggacccgtg agctcaggag
     5761 gccttgatgc cacgccagtc gttgccgagc cggtgctccc acgggagcac cgggcggggc
     5821 gtgcccatgt cggcgcgggc ccacggcagg taccgcctgg tgcccgcgac ggtgacccgt
     5881 gcctccgagg gccgcttcgg ccggttcgag ttggagcccg tgatccagtt ccatgcgtac
     5941 agcgacttgg tcttgctcat ggaggcgaac ctacggatca ccctggtcca cccggtttcc
     6001 ccggtgtacg aggtccgttg cgcttcgctg tcccccacct gacgaggcgg agacggcgtt
     6061 ggccgagcgc cttacggcgc taggcgccgt ggcactgctt gaacttcttg ccggagccgc
     6121 aggggcaggg ggcgttgcgg ggggtcttgt cccagtcgct cttcaccacc ggggtgttga
     6181 cgacctcctc ctccaccgcc accgccgtgc cgtcgaccgc agcctgggcc cgagcagcct
     6241 cgaccatgcc gttgccggcc gacgagggat cgtcgggacc cgaggtctgc atctgggtga
     6301 ccggggtgtc cgccgcgggc tcgtcgacca cgacctgcac gtgcatgacg tacttcacga
     6361 agtcctgggc gatggacgtc atcatcaggc cgaacatctc gaaaccctcg cgctgccact
     6421 cgacgagcgg gtccttctga cccatggccc ggaggttgat gccctcctcg aggtagtcca
     6481 tctccagcag gtgctcgcgc cagtgctggt cgacgatctg cagcatcacc tggcgctcga
     6541 tctggcgcat ggtgtcggga ccgagctctt cctcacgctt ctcgtagtag gcggtggcgt
     6601 cgtcgaggag gagctcctgg agctcctccg tgttgtcggc atcggcgagc tgctcctcgg
     6661 tgagcccggt gggccagaac gaggcgatct ccatgtggag gccctcgacg tcccactcgt
     6721 cggtcgcctc ggacacgcag aacgtggcca ccgacgtgtc gatggcctcc tcgaggtagt
     6781 cgagggtctc ggagcgcaga tcggcctcct cgaggatctg gtcgcggcgc ttgtagatga
     6841 ccttgcgctg ctcgttcatc acctcgtcgt acttgagcac gttcttgcgg atctccgcgt
     6901 tgcgctgctc gacggtgttc tgggcccgtt cgatggcctt ggtgaccatg cgggcctcga
     6961 tcggcacgtc gtcggggagg gccttgccca tcacccagtt catggcgccg gtggcgaaga
     7021 gccgcatcag ctcgtcttcg agcgagaggt agaagcggct ctcgccgggg tcgccctggc
     7081 gcccggagcg gccgcggagc tggttgtcga tgcggcggga gtcgtggcgc tcggtgccga
     7141 gcacgtagag gccgccgagg tcacggacct tggtgccctc ggcatcggtc tcgaccttgt
     7201 actgcttggt gagcgccttc aggcgctcgg cgccctcctc ggactccatg tcgacgccct
     7261 cggcgcgcag gtcccggatg gccaggccct ccgggttgcc gcccaggagg atgtcgacgc
     7321 cacgaccggc catgttggtg gcgacggtga tggagtgcag gcggccggcc tgggtgacga
     7381 tctcggcctc acgcgtgtgc tgcttcgcgt tgaggacctc gtgggcgatg ccccgcttgt
     7441 cgagcatccg c
//
LOCUS       DATMFS010000028         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_70834, whole genome shotgun sequence.
ACCESSION   DATMFS010000028 DATMFS010000000
VERSION     DATMFS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70834"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VNQ33_00540"
     CDS             <1..1435
                     /locus_tag="VNQ33_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96610.1"
                     /translation="DARWVAHQARADATVGRDAERLVAIAQARGGGRIFAGKLHDPAA
                     PAIGSVPGAIWLAHEPVDAIGFTLRVSALASDLESYLDATSVADLDAFGIRYVLVAGG
                     QRPPTGAWFLAASGDLQLWEVPGDGLISTARLLGSAKAVHRTELAADLLPLMRAQGGS
                     PASVRLLDLEGRSPAAPVQPQSSGAEHPGRIRSQDIELDAGRVGADVVFDEPGVLVVK
                     ANWNPGWHATVDGVDTAVVAVAPTWLAVPVTPGRHRIALTYRAPAGQAPLVVLAIVAI
                     ALGCAWSRRGRRARGEGRGRREGRERRVDRARVARRGRGPAAALVVLALVSCGTPHRL
                     APVRAVGPPQDSPLAEVELGRPDSFATLDRVLAPTTRTVVVVASSPADTFGLLEPLAD
                     AARGARGFSLLVLSPPDAEPDVADAAASLALDRWEVRDPVALDAATGLDTDSGVVLLD
                     EAGQVEGRWLPDAARDQLDTIIKEARR"
     gene            1432..2253
                     /locus_tag="VNQ33_00545"
     CDS             1432..2253
                     /locus_tag="VNQ33_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96611.1"
                     /translation="MTDRPTTQPHSPRRAARWPGAARWLTAVGTVALTLGVGPATVAS
                     AAPAGPALPAGADGGAAVACLDQRLRPAETCPSVDIEVHVEADGDDLVVQISNAAGRT
                     WRGGPLAVELPVPDAKVEEPEGRVDRTGRPGTPEWTTRVGTVVSRCVFEPVTELRSGA
                     DLSCRLRRLGLADGQHQIWFTGFIGGLPRVHGSVGNDAPAPGVVAAAATRSVRIQIEG
                     DRASVAASTPTAEAGETEGSRGGRSDGIGPWPPLIGAVALAVAVGVLARKRRRST"
     gene            2374..>2985
                     /locus_tag="VNQ33_00550"
     CDS             2374..>2985
                     /locus_tag="VNQ33_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96612.1"
                     /translation="MGQGTMKRAMLALAGATALVAMGGLDALPVSAGPIAGIPASSSF
                     VPGNPAVGATTSSTGTVPAGVCRGRIEARGGDGQQVEDGLAGTGSIIRGTFDVTPGQA
                     IDVRRHSGGRGGRGDGNPGGDGGDAIEVLVGGATQVVAGGGGGGANNSDDTANGSNNG
                     RGGNAGRAVATGVHNAGGGFGGAAGAPAGSTGGSGDGDNGAGGG"
BASE COUNT          350 a         1087 c         1141 g          407 t
ORIGIN      
        1 ggacgcccgc tgggtggcgc accaggctcg agccgacgcg acggtgggcc gcgacgcgga
       61 gcgcctcgtc gccatcgccc aggcccgggg cgggggtcgg atcttcgccg ggaagctgca
      121 cgaccccgca gctcccgcga tcggcagcgt ccccggggcc atctggctcg cccacgagcc
      181 ggtcgacgcc atcggcttca cgctgcgggt gtcggcgctg gcgtccgacc tcgagtcgta
      241 cctcgacgcc acctcggtgg ccgacctcga tgcgttcggc atccgctacg tcctcgtcgc
      301 cggaggccag cgaccgccaa cgggagcttg gttcctcgcg gccagcggcg acctccagct
      361 gtgggaggtg ccgggcgacg ggctgatctc cacggcccgg ctcctcgggt cggcgaaggc
      421 ggtgcaccgc accgagctcg ccgccgacct gctcccgctg atgcgggccc aaggcggctc
      481 gcccgcgtcg gtgcgcctgc tcgacctcga gggacggtcg cccgccgcgc cggtccagcc
      541 ccagagctcg ggggccgagc acccggggcg catccgatcg caggacatcg agctcgatgc
      601 cggccgcgtc ggcgctgacg tggtcttcga cgagcccggg gtgctggtgg tcaaggccaa
      661 ctggaacccg gggtggcacg ccacggtcga tggcgtggac acggcggtgg tggccgtcgc
      721 accgacgtgg ctggcggtcc ccgtgacgcc cggtcgccat cgcatcgccc tcacctaccg
      781 ggcgccggcc ggtcaggcac cgctggtggt gctcgccatc gtggccatcg ccttggggtg
      841 cgcttggtcc cgccgcggcc gccgggctcg cggcgaaggc cgcggtcgcc gcgaaggccg
      901 cgaacgccgt gtggaccggg ctcgtgtggc ccgacggggt cgaggaccgg ccgcggcgct
      961 ggtcgtcctc gcgctcgtga gctgcgggac cccgcaccgt cttgctccgg tgcgggccgt
     1021 gggtccaccc caggactcgc cgctggccga ggtggagctg ggtcgccccg actcgttcgc
     1081 cacgctcgac cgggtgctgg cgcccaccac ccggacggtc gtggtcgtcg cctcgtctcc
     1141 cgctgacacg ttcgggctgc tcgagcccct cgccgacgcc gctcgtgggg cgcggggctt
     1201 ctcgctgctc gtcttgtccc cacccgatgc cgaacctgac gtcgccgacg cggcggcgtc
     1261 gctcgccctg gaccgctggg aggtccgaga tcccgtggcg ctggatgcgg ccactggcct
     1321 cgacaccgac tcgggggtgg tcctcctcga cgaggccggg caggtcgagg gccggtggct
     1381 gccggatgcc gcccgtgacc agctcgacac catcatcaag gaggctcggc gatgaccgac
     1441 cgcccgacca cgcaacccca ctcgccgcgc cgtgccgccc gatggcccgg agcggctcga
     1501 tggctcaccg ccgtggggac ggtggccctc acgctcgggg tcggcccggc cacggtcgcc
     1561 agcgcggcgc cggccggtcc ggcgctccca gccggtgccg acggtggggc agcggtggcc
     1621 tgcctcgacc aacggctgcg cccggccgag acgtgtccct cggtcgacat cgaggttcac
     1681 gtcgaggcgg atggtgacga cctcgtggtc cagatcagca acgccgccgg acggacctgg
     1741 cgcggcgggc cgctggccgt cgagctcccg gtgcccgacg cgaaggtcga agagcccgag
     1801 ggccgcgtcg atcgaacggg tcgtcccggc accccggagt ggacgacccg ggtcggcacg
     1861 gtcgtcagcc ggtgcgtgtt cgaaccggtg accgagctcc ggtccggtgc cgacctctcc
     1921 tgtcgcctcc gccggctcgg cctcgccgat ggccagcacc agatctggtt caccggcttc
     1981 atcggtgggc ttccccgcgt gcacggttcg gtcggcaacg acgcccccgc ccccggcgtg
     2041 gtcgcggcgg cggcgacgcg ctcggtccgg atccagatcg aaggggaccg cgcctcggtg
     2101 gcagcatcga ctcccacggc ggaagcgggt gagaccgagg gctcccgcgg tggtcggtcc
     2161 gacggaatcg gtccgtggcc gcccctgatc ggcgcggtcg cgcttgctgt cgcggtcggc
     2221 gtcttggccc ggaaacgccg ccggtcgacc tgacgtcctc gaaaaatttc tgcgatcggc
     2281 gtcacggatg accagcgaga tggctcccta cgtgtcaggt ggacgaagtt ctgccgttgg
     2341 acgcgctgca cagcgccgag tgatggggtg gtcatgggcc aggggacgat gaagcgagcc
     2401 atgttggcgc tggcgggtgc gaccgccctg gtggcgatgg ggggtctcga tgcgctcccg
     2461 gtgagcgccg ggccgatcgc ggggatcccg gcctcgtcgt cgttcgtgcc ggggaacccg
     2521 gccgtgggcg ccaccacgag cagcacggga acggtgcccg ctggcgtgtg ccggggccgg
     2581 atcgaagctc gaggcggaga cggtcagcag gtcgaggacg gcctcgccgg cacgggatcg
     2641 atcatccgcg gcaccttcga cgtgaccccg ggccaggcga tcgacgtgcg ccggcactcc
     2701 ggcggccgcg gcggccgcgg cgacggcaac ccgggcggtg acggcggtga tgcgatcgag
     2761 gtcttggtcg gcggcgccac ccaggtggtc gctggcggcg gcggtggcgg tgccaacaac
     2821 tccgacgaca cggccaacgg ctcgaacaac gggcggggcg gcaacgccgg ccgcgccgtc
     2881 gccactggcg tccacaacgc cggtggcggg ttcggcggcg ccgcgggggc cccggcaggg
     2941 agcaccggcg gttcgggcga tggcgacaac ggtgctggtg gcggc
//
LOCUS       DATMFS010000029         3672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_187204, whole genome shotgun sequence.
ACCESSION   DATMFS010000029 DATMFS010000000
VERSION     DATMFS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187204"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..823)
                     /locus_tag="VNQ33_00555"
     CDS             complement(125..823)
                     /locus_tag="VNQ33_00555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWJ96613.1"
                     /translation="MARTHTEPLPTGDDKVVAVRSMFDAIAPRYDLVNRLMTFRMDVG
                     WRKKAIASLGLPTGSTVLDLACGTGDLCRGLAGAGLRPVGLDLSYGMLAAARTDAPLA
                     QADALRLPVPDGSVDGVTCGFALRNFAALEPFFAELGRVVRPGGRIALLEVAEPPNPV
                     LRWGHGIYFGKVVPVIGGLLSDGAAYRYLPKSVAYLPEPEVMVDQLRGAGFTAVDRRL
                     LSTGISQLLTATRA"
     gene            862..1566
                     /gene="yaaA"
                     /locus_tag="VNQ33_00560"
     CDS             862..1566
                     /gene="yaaA"
                     /locus_tag="VNQ33_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HWJ96614.1"
                     /translation="MARRSLILLPPSEGKAPEGDGPPWAPGTMAVPELDAARKKVLAA
                     LGRRHPARTGPTLPAIERYTGVLYQGLDAGSLGAVARRRLDRSVLVVSGLWGLVAPQD
                     PIPHYKLKMSASVAPLGKLSTWWRPAVTAAVAARAERALVWDLLPIEHAAAVAWDGIR
                     PYERVTVRFVDAQGKTVSHWNKLLKGSLVRWLVTTGSTDVDALTGFEHPQGYRYDPAA
                     TVRDGRLTALTFRAPA"
     gene            1642..2727
                     /locus_tag="VNQ33_00565"
     CDS             1642..2727
                     /locus_tag="VNQ33_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96615.1"
                     /translation="MLLYELAITLDRRSTELVDAATELGLGHLQAGSDLDAKQLAALR
                     AHFGAGVAPPPLAPLGSAPPLPAPPTAPLAFGAPPPPVGAPVGAPTAPAAWGPPTAPG
                     RWGPPPAAVPPAAPPDGAPPATAAHIGPAFAYPDSPIAQEAAPAGAPSPASGGPLPPP
                     PDPVPGAAPASAPGFGTGQKVLIGAVVVGVIALFGFMAVNTGPDKERQQALATKQAEL
                     EADLNATTAPIATTTEAPATPAAPPTAYDIVDVTRFCKGGLGITTFELRLAAALADQD
                     FEELSSIVRDRRAAWSDDVASLVESAPPILVDDIELYRDGYNTFFDAVASSSSMEQAY
                     RKVDRMQLIKAGNAGQEVSTQIANECK"
     gene            2779..3528
                     /locus_tag="VNQ33_00570"
     CDS             2779..3528
                     /locus_tag="VNQ33_00570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ96616.1"
                     /translation="MSTERDHGFEDLADHLGSAYLRYSFTKGTEQEVGFLVDVLGLEP
                     GMRVLDVGCGPGRHAHALGRRGIEVHGIDISQRFVDLAREGAPQGATFERLDARTLPF
                     DAEFDAAISLCQGAFGLADWGEAVDKLDPDVEILECIARALKPGGRAGVTAFSAYFQV
                     RHVEAMGSEFDAERGVNHERATIKDEQGNDAEHDLWTTCFTPRELRLMAKQAGLTVEE
                     IWSVTPGGYTRDKPTLESEEFLLVAGTPAHL"
BASE COUNT          547 a         1362 c         1305 g          458 t
ORIGIN      
        1 cggccggcga agcccacgcc gctgcgggcg aacagcacgc cgtcgtcgcc cgccagtccg
       61 aggaggtccg ggaccgcttc cgggccgagc gggcgggtgc gggcgacgag cccggcgggg
      121 tgcgtcacgc ccgggtcgcg gtcagcagct gcgagatgcc cgtcgacagc agccggcggt
      181 cgaccgccgt gaacccggcg ccccggagct ggtcgaccat gacctcgggc tcgggcaggt
      241 aggcgacgga cttcgggagg tagcggtagg cggcgccgtc ggagaggagg ccgccgatga
      301 ccggcaccac cttgccgaag tagatgccgt ggccccagcg caggacgggg ttgggcggct
      361 cggccacctc gagcagggcg atgcggccac cggggcggac cacgcggccg agctcggcga
      421 agaagggctc gagcgcggcg aagttccgga gggcgaagcc gcacgtgacg ccgtcgaccg
      481 aaccgtcggg gaccggcagg cggagggcgt cggcttgcgc cagcggtgcg tcggtgcggg
      541 ccgcggcgag catcccgtag gacaggtcga ggccgacggg ccgcaggccg gcgcccgcca
      601 ggccgcggca gaggtcgccg gtgccgcacg ccagatcgag cacggtcgaa ccggtcggga
      661 ggccgagcga ggcgatggcc ttcttccgcc agccgacgtc catgcggaac gtcatgagcc
      721 ggttcacgag gtcgtagcga ggggcgatcg cgtcgaacat cgaccgcacg gccaccacct
      781 tgtcgtcccc ggtgggcagc ggttccgtgt gcgttcgagc catgttcggg atcgtacggc
      841 ggatcgctac tgtcgccgcc catggcccgc cgctcgctga tcctcctgcc gccctccgag
      901 gggaaggcgc cggagggcga cggaccgccg tgggctcccg ggaccatggc cgtccccgag
      961 ctcgacgccg ctcggaagaa ggtgctcgcc gcgctcggcc gccggcaccc ggctcggacc
     1021 ggcccgaccc tgcccgccat cgagcgctac acgggcgtgc tctaccaggg actcgacgcc
     1081 ggctcgctcg gcgccgtggc ccgccgccgg ctggaccgct cggtgctggt cgtctccggc
     1141 ctctggggcc tggtcgcccc acaggacccg atcccgcact acaagctcaa gatgagcgcc
     1201 agcgtggccc cgctggggaa gctgtcgacc tggtggcgcc cggcggtgac cgcggcggtg
     1261 gcggcccgtg ccgagcgggc gctcgtgtgg gacctgctgc cgatcgagca cgcagcggcc
     1321 gtcgcctggg acggcatcag gccctacgag cgggtgacgg tgcgcttcgt cgacgcgcag
     1381 ggcaagacgg tcagccactg gaacaagctg ttgaagggct cgctggtgcg gtggctcgtc
     1441 acgaccgggt cgaccgacgt cgacgccctg accggcttcg agcacccaca gggctaccgg
     1501 tacgaccccg cggccaccgt gcgcgacggc cggctgaccg ctctcacctt ccgtgctccg
     1561 gcctgacagc accgtcaccg ctcaagcgcg gcgcccgcgg tccgatggga ggggatcccc
     1621 cgatcggcga cgaggtaccc ggtgctgctc tacgaactgg ccatcacgct cgacaggcgc
     1681 tcaaccgaac tggtggacgc cgccaccgag ctgggcctgg gtcacctcca ggccggttcc
     1741 gacctggacg cgaagcagct cgccgcgctc cgggcccact tcggagccgg cgtggcaccg
     1801 cctccgctcg ccccgctcgg ctcggccccg ccgcttcccg cgccgccgac ggcgccgctg
     1861 gccttcggcg ccccgcctcc acccgtcggc gctccggttg gcgcgccgac ggcgcccgcc
     1921 gcgtggggtc cgcccaccgc acccgggcgt tggggacccc ctcccgcggc cgtgccgccc
     1981 gctgcgccgc ccgacggcgc gccgcccgcc accgcggcgc acatcgggcc ggcgttcgcc
     2041 taccccgaca gccccatcgc ccaggaggcc gcgccggcgg gagcgccgtc gcccgcgtcc
     2101 ggcggcccgc tgcctccccc gccggacccg gtgcccggtg ccgcaccggc gtcggcgccg
     2161 gggttcggga ccgggcagaa ggtgctgatc ggtgccgtcg tcgtgggggt gatcgccctg
     2221 ttcggcttca tggccgtgaa cacgggtccg gacaaggagc ggcagcaggc cctggcgacg
     2281 aagcaggcgg agctcgaagc cgacctcaac gcgacgaccg cgccgatcgc cacgacgacc
     2341 gaggctcccg ccacccccgc ggccccgccg acggcctacg acatcgtgga cgtgacccgc
     2401 ttctgcaagg gaggcctcgg catcacgacc ttcgagctgc gcctcgcggc cgccttggcc
     2461 gaccaggact tcgaggagct gagcagcatc gtccgcgacc gccgtgcggc atggagcgac
     2521 gatgtcgcgt ccctcgtgga gagcgcgccc ccgatcctcg tggacgacat cgagctctac
     2581 cgagacggct acaacacctt cttcgacgcc gtcgccagca gctcgagcat ggagcaggcc
     2641 taccgcaagg tcgaccggat gcagctgatc aaagcgggca acgccggcca ggaggtcagc
     2701 acccagatcg ccaacgagtg caagtagggg ccggagctga ccgggaaccg gcgggggccc
     2761 agctggcatg ctcggccggt gagcacggaa cgagaccacg gcttcgagga cctggcggac
     2821 cacctgggct cggcgtacct gcgctactcg ttcaccaagg gcaccgaaca ggaggtcggc
     2881 ttcctggtcg acgtcctcgg cctggagccc ggcatgcggg tcctcgacgt ggggtgcggc
     2941 ccgggccgcc acgctcacgc cctgggccgc cggggcatcg aggtccatgg catcgacatc
     3001 agccagcgct tcgtcgacct ggcgagggaa ggcgcaccgc agggcgcgac cttcgaacgg
     3061 ctcgacgccc gcaccctccc gttcgacgcc gagttcgacg ccgccatctc cctgtgccaa
     3121 ggagccttcg gcctcgccga ctggggtgaa gccgtcgaca agctcgaccc cgacgtcgag
     3181 atcctcgaat gcatcgcgag agccctcaag ccaggaggaa gggccggcgt caccgccttc
     3241 agcgcctact tccaggtgag gcacgtcgaa gccatgggca gcgagttcga cgccgaacga
     3301 ggcgtcaacc acgagcgggc gacgatcaaa gacgagcagg gcaacgacgc agagcacgac
     3361 ctctggacca cctgcttcac ccccagagag ctgcgcttga tggccaagca agcaggcctg
     3421 accgtcgaag agatctggag cgtcacccca ggcggctaca cccgtgacaa gccgaccctg
     3481 gaaagcgagg agttcctgct ggtggcagga accccagctc acctttgaga tcaccggtgg
     3541 gggactggta cctttggact tccctgtgtg ccgtgtggcc gcaggggttc ccatccatcg
     3601 acttcctgtc cacctgagaa gagcatccgt gtccgagtca tcgaccctca ccccgcccgc
     3661 cgagaccgac ga
//
LOCUS       DATMFS010000030         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_303315, whole genome shotgun sequence.
ACCESSION   DATMFS010000030 DATMFS010000000
VERSION     DATMFS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303315"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /gene="folE"
                     /locus_tag="VNQ33_00575"
     CDS             complement(<1..577)
                     /gene="folE"
                     /locus_tag="VNQ33_00575"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWJ96617.1"
                     /translation="MSEPERPVDQPRIEAAVREILAAIGEDPDRDGLQKTPARVARMY
                     AEICAGLHQNPDEHLQTTFEAEHDEMVMVRDIPLYSLCEHHLAPFIGKAHVAYIPGTD
                     GRVIGLSKFARLVDGYARRLQVQERLTSQIADAMQNSLDPKGVMVVIEAEHLCMSMRG
                     VKQAGSTTVTSAVRGLFRTNVATREEAMRFIQ"
     gene            complement(574..1155)
                     /locus_tag="VNQ33_00580"
     CDS             complement(574..1155)
                     /locus_tag="VNQ33_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96618.1"
                     /translation="MSRMRTLGLGVLAATVLFGAAACSKSDGGTKKSDRAPISFADPP
                     KGSMDIGICYAYNTEQIKDLIGGKETFKRLAPAAIGTAKDKVHGEACGWQRTEPNGDS
                     VNLRVEIRDFGEDQAALLTQYDALKKGATDVTDVAGLGEGAFSAVSDDTSLLEVKSGP
                     YLLTLTSHAEGSLKPVDLAALQLLAASGLDRIT"
     gene            complement(1241..1795)
                     /gene="hpt"
                     /locus_tag="VNQ33_00585"
     CDS             complement(1241..1795)
                     /gene="hpt"
                     /locus_tag="VNQ33_00585"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWJ96619.1"
                     /translation="MTDAPAGPYEGDPHIGKVVVSTEELQRRTAELGAEITADYQGRA
                     PLLVGVLKGAIMFMADLAREIALPVEFDFMAVSSYGNATKSSGVVRIVKDLDLDLSGR
                     HVLIVEDIIDSGLTLAYLRKNLLARHPASVEVCALLVREGLQKKDPDLKYVGFRIPPD
                     FVVGYGLDVRDRYRNLPYVASYLA"
     gene            complement(1788..>2477)
                     /locus_tag="VNQ33_00590"
     CDS             complement(1788..>2477)
                     /locus_tag="VNQ33_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ96620.1"
                     /translation="GAGFRRERVEVGDGPNLEARARAARYAVLPDGVLTGHTADDRAE
                     TVLLHLLRGAGPTGAVGIGRSPQRPLLDLRRADTEALCVALGFDPVHDPSNDDPRFRR
                     NRIRHELLPLMASIGERDPVPVLVRQADLFADVDAAIGRAAARLDTTVAADLAAAPAA
                     VAGAAVRAWLVAAGVGDGYGIDGASVARVLAVARGERVATEVAGGWRIARSRGRLSVT
                     APGSWQDAAHD"
BASE COUNT          338 a          852 c          920 g          367 t
ORIGIN      
        1 tctggatgaa gcgcatggcc tcttcacggg tggcgacgtt ggtgcggaac agccctcgca
       61 cggccgacgt cacggtggtg gatccggcct gcttgacccc gcgcatcgac atgcagaggt
      121 gctcggcctc gatgaccacc atcacgccct tggggtccag gctgttctgc atggcgtcgg
      181 cgatctggct ggtcaggcgc tcttgcacct ggagacgccg cgcgtagccg tcgacgaggc
      241 gggcgaactt cgacaagccg atgacccggc cgtcggtccc ggggatgtag gccacgtggg
      301 ccttcccgat gaagggcgca aggtggtgct cgcacagcga gtacagcggg atgtcgcgca
      361 ccatcaccat ctcgtcgtgt tcggcctcga acgtggtctg gaggtgctcg tcggggttct
      421 ggtggagccc ggcacagatc tcggcgtaca tgcgggcgac gcgcgccggc gtcttctgga
      481 gaccgtcgcg gtcggggtct tccccgatgg cggccaggat ctcccgaacg gctgcctcga
      541 tgcgaggctg gtcgaccggg cgctccggct cgctcacgtg atccgatcca gtcccgacgc
      601 ggccagcagc tgcagggcgg cgaggtccac cggcttcagg ctgccctcgg catggctggt
      661 gagggtgagc aggtacggcc cggacttgac ctcgaggagc gacgtgtcgt cgctcaccgc
      721 ggagaaggcg ccctcaccga ggccggcgac gtcggtgaca tcggtggcgc ccttcttgag
      781 ggcgtcgtac tgggtgagca gcgccgcctg atcctcgccg aagtcgcgga tctcgacgcg
      841 caggttcacc gagtcgccgt taggctcggt gcgctgccag ccgcacgcct caccgtggac
      901 cttgtccttc gcagtgccga tggctgcggg ggcaaggcgc ttgaacgtct ccttgccgcc
      961 gatcagatcc ttgatctgct cggtgttgta cgcgtagcag atgccgatgt ccatcgagcc
     1021 cttgggcggg tcggcgaacg agatcggcgc ccggtcgctc ttcttcgtgc cgccatcgct
     1081 cttgctgcag gcggccgcgc cgaacagcac cgtcgccgcc agcactccga gcccgagggt
     1141 ccgcatccgt gacatgggag gcgacgctag cgccgcgggc tgtggcggcc cacgtcagcg
     1201 gcccacgtca gcggcccgcc gtcgagggcc gccggggcgg tcacgccagg tacgaggcga
     1261 cgtacgggag gttgcggtag cgatcgcgga cgtcgaggcc gtagccgacc acgaagtccg
     1321 gcgggatccg gaagccgacg tacttgaggt ccgggtcctt cttctggagc ccctcgcgca
     1381 cgagcagggc gcacacctcg acgctggccg ggtgacgggc caggaggttc ttgcgcaggt
     1441 aggcgagggt gagaccgctg tcgatgatgt cctcgacgat caggacgtgg cgaccgctca
     1501 gatcgaggtc gaggtccttg acgatgcgga cgacgccgct ggacttggtg gcgttgccgt
     1561 acgacgacac cgccatgaag tcgaactcga ccggcagggc gatctcccgg gccaggtcgg
     1621 ccatgaacat gatggccccc ttcagcacgc cgaccaggag cggcgcccgg ccctggtagt
     1681 cggccgtgat ctccgcaccg agctccgccg tgcggcgctg gagctcctcg gtgctcacga
     1741 ccaccttgcc gatgtgcggg tcgccctcgt agggcccggc cggggcgtca gtcatgggcg
     1801 gcatcctgcc atgagccggg ggcggtgacc gacaggcgcc cgcgggaccg ggcgatgcgc
     1861 catccgccgg cgacctcggt ggcgacgcgc tcgccgcgag cgaccgccag cacccgggcg
     1921 acggacgcac cgtcgatccc gtacccgtcg cccacgccgg ccgccaccag ccaggcgcgc
     1981 acggcggccc cggcgacggc ggcgggagcg gcggcaaggt cggcggcgac cgtggtgtcc
     2041 agccgggcgg cggcgcgtcc gatggcggcg tcgacgtcgg cgaacagatc ggcctggcgg
     2101 accagcacgg gcaccgggtc gcgctcgccg atgctggcca tgagcgggag cagctcgtgg
     2161 cggatccggt tgcggcggaa ccgcgggtcg tcgttcgacg ggtcgtggac cggatcgaac
     2221 ccgagcgcca cgcagagcgc ctcggtgtcg gcgcggcgca ggtcgagcag cggccgctgc
     2281 ggagaccggc cgatgccgac cgcccccgtg ggccccgctc cgcggaggag gtggagcagc
     2341 accgtctcgg cccggtcgtc ggccgtgtgg ccggtgagga cgccatcggg cagcaccgcg
     2401 tagcgggcgg cccgagcccg ggcttcgagg ttcggtccgt cgcccacctc gacccgctcc
     2461 cgtcggaacc cggcgcc
//
LOCUS       DATMFS010000031         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_280173, whole genome shotgun sequence.
ACCESSION   DATMFS010000031 DATMFS010000000
VERSION     DATMFS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280173"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="VNQ33_00595"
     CDS             complement(<1..2009)
                     /locus_tag="VNQ33_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ96621.1"
                     /translation="MAQLSNPWPLARRAAEQDAFIAALDQDGTNVVVLYGTAGVGKSR
                     LADAFLDAAEEHGRLTARVTGSTAAALMPLGALAPVLPHDLDATATARDLFERTRSQL
                     QSLGGDGRLVLMVDDAHLLDTSSAVLLTQLIDAGAVFVVATIREGEDLPDAVASWWRS
                     QRAVRVDLEDLDRPGTGDVLRLALGGEVGADTVRRLHDASGGNPLLLRELVIQALGAD
                     RLKDDSGTWRLVGPIPPSSRLSELFAVRLGQLGDDARHVLEQLALCAPLGPAELTGSR
                     TDRDLEELERAGLIRVLLDDHRQVLTLAHPLYGEALRANLPVLRRRSILLAAAERVEE
                     LGARRREDPRRIATWRLDAGGSPDPVLLLQAATVARYAHDFVEVERLADVLMRTAPSA
                     EAALLLGEAHYELGHFDEAEAVLAASMPDATPVDLLTRQATLRTKNLQWGLCDWAAAM
                     EVVDAAIAAIGPDDGVDVVAEKGSVLLFSGHPQQALDLFDSIEVRTPRTQVLIALVES
                     PALAAVGQTARAVEVAVDGFAAHRAMDDPLALAHPGTHLVNQVFAMVEAGRLDEADAL
                     AGLGYEVAVADAVPIAQIWFAVMLGKSSGLRGRMDESLRWYQEGASTARLHGFRGPLS
                     GALAGLAVATANLGRAAASAAAIDEYRSLPPFPFFGHDHAIGIAWA"
     gene            2178..2378
                     /locus_tag="VNQ33_00600"
     CDS             2178..2378
                     /locus_tag="VNQ33_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96622.1"
                     /translation="MGFFKPAGDDGAEAGAAAGDEDATGASGDDGALSASAEDEGSEA
                     LTDLGVDLAADAGDDDGSAAGL"
BASE COUNT          363 a          932 c          990 g          307 t
ORIGIN      
        1 gcccaggcga tgccgatggc gtggtcgtgg ccgaagaacg ggaacggcgg cagcgaccgg
       61 tactcgtcga tggccgcagc cgaagcagca gcgcggccga ggttggcggt ggcgacggcg
      121 aggccggcga gcgcacccga cagcgggccc cggaagccgt gcagccgcgc cgtcgaggcg
      181 ccctcctggt accagcggag cgactcgtcc atccggcccc ggagcccgga ggacttcccg
      241 agcatcaccg cgaaccagat ctgggcgatc gggacggcgt cggcgacggc gacctcgtag
      301 cccaggccgg cgagcgcatc ggcctcgtcg agccggcccg cttccaccat ggcgaacacc
      361 tggttgacga ggtgggtgcc ggggtgggcg agggcgagcg gatcgtccat cgcccggtgc
      421 gcggcgaagc cgtcgacggc cacctccacc gcccgcgccg tctggccgac ggcggcgagc
      481 gccgggctct ccacgagggc gatgagcacc tgggtgcggg gcgtgcgcac ctcgatcgag
      541 tcgaacaggt cgagcgcctg ctgcgggtga ccggagaaca gcagcaccga gcccttctcg
      601 gccacgacgt cgacgccgtc gtcggggccg atcgccgcga tggccgcgtc gacgacctcc
      661 atcgcggcgg cccagtcgca cagcccccac tggaggttct tggtgcgcag cgtggcctgc
      721 cgggtcagca ggtccaccgg cgtggcgtcg ggcatcgacg cggccagcac cgcctccgcc
      781 tcgtcgaagt ggcccagctc gtagtgggct tcgcccagca gcagggccgc ttcggcggac
      841 ggagccgtgc gcatcaacac gtcagccagg cgttcgacct cgacgaagtc gtgggcgtac
      901 cgggcgacgg tggcggcctg cagcaggagc accgggtcgg gtgagccgcc ggcatcgagc
      961 cgccacgtgg cgatccgccg agggtcctcg cggcggcggg cgcccagctc ctcgacccgc
     1021 tcggcggcag ccagcaggat cgagcgccgg cgcagcaccg gaaggttggc ccggagcgcc
     1081 tcgccgtaga gcggatgggc gagcgtcagc acctggcggt ggtcgtcgag caggacccgg
     1141 atcagcccgg cccgctcgag ctcctcgagg tcccggtcgg tccgcgagcc ggtcagctcc
     1201 gcggggccga gcggcgcgca cagcgcgagc tgttcgagca cgtggcgggc atcgtcgccg
     1261 agctggccga ggcggacggc gaacagctcg ctcaggcgtg agctcggggg gatcgggccg
     1321 acgaggcgcc aggtgcccga atcgtccttg aggcggtccg cgcccaacgc ctggatcacc
     1381 agctcccgca gcagcagggg gttgccgccg ctggcgtcgt ggaggcggcg cacggtgtcg
     1441 gcgcccacct ccccgccgag cgcgaggcgg aggacgtccc cggtgccggg ccgatcgagg
     1501 tcctcgaggt cgacgcgcac ggctcgctgg ctgcgccacc agctcgccac cgcgtcgggc
     1561 aggtcctcgc cctcgcggat ggtggccacc acgaacacgg ccccggcatc gatcagctgg
     1621 gtcaggagca cggcggacga cgtgtccagc aggtgggcgt catccaccat gaggaccagc
     1681 cggccgtcgc cgccgagcga ctgcagctgc gaccgcgtgc gctcgaacag gtcgcgggcc
     1741 gtggccgtgg cgtcgaggtc gtgggggagc accggagcga gcgcgccgag cggcatcaag
     1801 gcggcggccg tgctccccgt cacccgggcg gtgagccgcc cgtgctcctc ggcggcatcg
     1861 aggaacgcgt cggccaggcg ggacttgccc accccggccg ttccgtacag gaccacgacg
     1921 ttggtgccgt cctggtcgag cgcggcgatg aaggcgtcct gctcggcggc gcgccgggcg
     1981 aggggccacg ggttgctcag ctgtgccatg cgccccgccc cgctgttgga tccacggcgc
     2041 cgaacgtacc tcgccacggc atctgctgga cccggacaga ttgagcagtg cctactcatg
     2101 accgcctccg cggatggagc cagcatcgga gacgtcggcc ggagcgcgcg gccgcgagat
     2161 cgaggagacg cagtccgatg gggttcttca agccggccgg cgatgacggg gcggaagcag
     2221 gtgcggcggc cggagacgag gacgccaccg gcgcgtccgg cgacgacggc gccctgtcgg
     2281 cgtcggccga ggacgaaggg agcgaggcgc tgaccgacct gggcgtcgac ctcgccgccg
     2341 atgccggcga cgacgacggc tccgccgccg gcctctgacc cggaggtcgc cgcccccggt
     2401 ctgtgctgcc atgctgggga cggccggacc ggagaggagc caccccatgc ccgacgacga
     2461 tcgaccaccg ttccacctgg cgttcccgtg ccgggacgtg gtcgaggcgg tcgccttcta
     2521 cgagggcctc ggcgccacgg tcggccggca caacgaccac gccgccatct tcgggttctt
     2581 cggccaccag at
//
LOCUS       DATMFS010000032         4581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_47528, whole genome shotgun sequence.
ACCESSION   DATMFS010000032 DATMFS010000000
VERSION     DATMFS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47528"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VNQ33_00605"
     CDS             complement(<1..987)
                     /locus_tag="VNQ33_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWJ96623.1"
                     /translation="MSLLLSLLAGVVLGAAVERVVGRSPAPATTANANVAPAPERTRR
                     WGRIVGVTALVLAVALVAGALVMYLWASNVFDKIEKVPVASVLSDDTSAGTNYLLVGS
                     DNGEEGGEQREGVEGRRSDTIMVLNVKDGKAKMLSLNRDLWVENPATGANGRLNAAYS
                     AGPANLVEAVTQNFGIPINHYIEIDFISFGGLVDSFGGIDISFEHPAFDLASGLDVKQ
                     AGLVHLDGQQALAYVRSRHYAEVIDGEVVLDPTSDLGRVQRQQVFLREIMNKAGAKRN
                     PFTLMKAAENMSGGLRIDDTMTMWEAARFAWAMGRLDPVSVPLPVSPRTTSGG"
     gene            complement(984..1850)
                     /locus_tag="VNQ33_00610"
     CDS             complement(984..1850)
                     /locus_tag="VNQ33_00610"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HWJ96624.1"
                     /translation="MLPLAAIAVGALIGFVGSRKLRRPQHAAQAASVARTVSASVGRR
                     RTLTAPELQRACFSEMVRHVRVDRQGRTRAPSRYVIQVAAVDMEVIDDTRSWFTDGLR
                     DALTTAAHDNGWTIDGTIAISFEVDASRRAGVPNALAVDPEGPKRAEPTAPPGQAPAP
                     TSLALVRTDTGARTALDRDAVTIGRSSDRTITIDDTRVSRSHATVERTRSGWSVTDNG
                     SSNGTRVNGKAIAAKAPKALDDGDVIGVGPVDLRVEAAAPEGAAGTRALDDSDRTRIS
                     GEVLPPPRRSEP"
     gene            complement(1868..2170)
                     /locus_tag="VNQ33_00615"
     CDS             complement(1868..2170)
                     /locus_tag="VNQ33_00615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWJ96625.1"
                     /translation="MIVVTGYLTIDPDKRADAEAAIAAFVPKNLAEEGCVDYRFSFDV
                     LEPNRLNGLEQWESQAAIEAHMASDHMAEFMAAIGPCLGGEAAFTAHEISSSTKII"
     gene            complement(2247..3662)
                     /locus_tag="VNQ33_00620"
     CDS             complement(2247..3662)
                     /locus_tag="VNQ33_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWJ96626.1"
                     /translation="MPAHRVRSSAPLPLDFAAIRQELDVPGAFPAEALAEADHAAARR
                     FPDHEDATHLDLVTIDPPGSRDLDQAVAIETTDDGWQVSYAIADVAAWVDPGGAVDQA
                     ARERTQTYYAPDARMPLHPPVLGEGAASLLPDGPRPAALWTIGVDRGGVTTSVDVRRA
                     LVRSRAQLTYREVQDQLDAGKAPAAIAAFPDIGAALLAAARERGAIELGLSDQEVVPA
                     GDGSWTVKLRADLPVEHWNAQISLLTGRAAAWLMIETGTGILRTVPQADPATFPRLQQ
                     AARSLGISWPDGMQPGAVLASLDTSRPRHAAFADLAAELLRGAAYTPFTGAPPADPGH
                     AGVGAPYAHVTAPLRRLVDRFGTEICLAAVRGGAVPSWAADALPDLPDQMAAGDARAK
                     KLDRAVVDATEVVVLADRVGDVFPAAVVETGDRYGTVVLDEPPVRARCDTRHLPLGEE
                     IHVRCTVADPATRTVRFERVS"
     gene            3703..>4581
                     /locus_tag="VNQ33_00625"
     CDS             3703..>4581
                     /locus_tag="VNQ33_00625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ96627.1"
                     /translation="MSDSPVADYPDRWESEVVLSDGGTMALRPIRPDDAERIVAFHER
                     QSPESIYFRFFSPRPRLSERDLERFTRVDYIDRMAFVGLIGDELVGVARYDRHRARSD
                     AEVAFFIDDAHHGRGMATVLLEYLAEAAREAGISGFTASVLPQNRKMLGVFTQAGFTA
                     SSRFEDGVIEVQLSIEPTPAALAAIEVRAGVAEARSVQRLLRPTSIAVVGASRTPGTI
                     GHQVFHELVSRGFQGPVYPVNREADYVASVRSWRSVLDVPGELDLAIVCVPAGEVLRV
                     AEECAYKRVQGLVVLSA"
BASE COUNT          597 a         1520 c         1815 g          649 t
ORIGIN      
        1 accgccgctg gtggtgcggg gcgacaccgg cagcggcacc gagacggggt cgagccggcc
       61 catcgcccag gcgaagcggg cggcctccca catcgtcatg gtgtcgtcga tgcggaggcc
      121 gccgctcatg ttctcggccg ccttcatcag cgtgaacggg ttccgcttgg cgccggcctt
      181 gttcatgatc tcccgcagga acacctgctg gcgctggacc cggccgagat cgctggtggg
      241 gtcgagcacg acctcgccgt cgatcacttc ggcgtagtgg cgggagcgca cgtaggcgag
      301 ggcctgctgg ccgtcgaggt ggaccaggcc ggcctgcttc acgtccaggc ccgacgccag
      361 gtcgaacgcg gggtgctcga acgagatgtc gatgccgccg aacgagtcga ccagcccgcc
      421 gaacgagatg aagtcgatct cgatgtagtg gttgatcggg atcccgaagt tctgggtgac
      481 ggcctcgacg aggttggccg gcccggcact gtaggcggcg ttcaggcgtc cgttcgcgcc
      541 cgtggccggg ttctcgaccc acagatcccg gttcagggag agcatcttgg ccttcccgtc
      601 cttcacgttc agcaccatga tcgtgtcgga gcggcggccc tcgacgccct cgcgctgctc
      661 gccgccctcc tcgccgttgt cgctgccgac cagcaggtag ttggtgccgg ccgacgtgtc
      721 gtcggacagg accgacgcga cggggacctt ctcgatcttg tcgaagacgt tggatgccca
      781 caggtacatg accagcgccc cggccacgag ggcgaccgcg agcacgaggg cggtgacgcc
      841 gacgatgcgg ccccagcggc gcgtgcgctc gggagcggga gcgacgttgg cgttggccgt
      901 ggtggcgggc gcgggcgagc ggccgacgac gcgctcgacc gcggcaccga ggacgacgcc
      961 ggccaggagg gagaggagca ggctcacggc tcggaccgcc ggggcggggg caggacctcg
     1021 cccgagatgc gggtgcggtc gctgtcgtcg agcgcccggg taccggctgc gccctcgggg
     1081 gcggcggcct cgacgcgcag gtcgaccgga ccgacgccga tcacgtcgcc gtcgtcgagc
     1141 gccttcggcg ccttggcggc gatggccttc ccgttgacgc gggtgccgtt ggacgagccg
     1201 ttgtcggtga cgctccagcc cgaccgggtg cgctcgacgg tggcgtgcga ccggctgacc
     1261 cgggtgtcgt cgatggtgat ggtgcggtca gaggagcgcc cgatggtgac ggcgtctcgg
     1321 tccagggcgg tgcgagcgcc ggtgtcggtg cgcacgagcg ccaggctggt gggtgccggc
     1381 gcctggcccg gcggcgcggt gggctcggcc cgcttcggcc cctcggggtc cacggcgagg
     1441 gcgttgggga ccccggcgcg acgcgaggca tcgacctcga agctgatggc gatggtcccg
     1501 tcgatcgtcc agccgttgtc gtgcgccgcg gtggtgaggg cgtcacggag cccgtcggtg
     1561 aaccaggatc gggtgtcgtc gatgacctcc atgtcgaccg cggccacctg gatcacgtag
     1621 cgcgacggtg cccgggtgcg gccctggcgg tcgacgcgga cgtggcggac catctccgag
     1681 aagcaggctc gctgcagctc cggagcggtg agggtcctgc ggcggccgac gctggcgctg
     1741 acggtgcgcg cgacggacgc cgcctgggcg gcgtgctgcg ggcgacggag cttgcgggag
     1801 ccgacgaacc cgatgagggc accgacggcg atggccgcga gggggagcaa ggggggaggc
     1861 gctcctttta gatgatcttc gtcgaagacg agatctcatg ggcggtgaag gcggcctcgc
     1921 cgccgaggca cggcccgatc gcggccatga actcggccat gtggtcgctc gccatgtggg
     1981 cttcgatcgc cgcctgcgac tcccactgct cgaggccgtt gaggcggttc ggctccaaga
     2041 cgtcgaagga gaaccggtag tcgacgcagc cctcctcggc gaggttcttc ggcacgaacg
     2101 cggcgatggc ggcctcggcg tcggcgcgct tgtccggatc gatggtgagg tagccggtga
     2161 cgacgatcat gggagcggtc cttctgcggg ggagggtcga tcaaggttag aggggcggac
     2221 ccgccgaaat cggacacccc caggggtcag gagacgcgtt cgaagcggac cgtgcgggtg
     2281 gcggggtcgg ccacggtgca gcgcacgtgg atctcctcgc ccaacggcag gtgcctggtg
     2341 tcgcagcggg cccggacggg cggctcgtcg agcaccacgg tcccgtagcg gtcgccggtc
     2401 tcgacgaccg cggcggggaa cacgtcgccc acccggtcgg ccaagacgac gacctcggtg
     2461 gcgtcgacga cggcgcggtc cagcttcttg gctcgagcgt cgccggcggc catctggtcg
     2521 ggcaggtcgg gcagggcgtc agccgcccac gacgggacgg cgccgccccg gacggcggcc
     2581 aggcagatct cggtgccgaa ccggtcgacg aggcggcgga gcggcgcggt gacgtgggcg
     2641 tagggggcgc cgacgccggc atggccggga tcggccggtg gtgccccggt gaacggggtg
     2701 tacgcggcgc cgcggagcag ctcggcggcc aggtcggcga acgcggcgtg gcggggccgg
     2761 ctcgtgtcca gcgacgccag cacggcgccc ggctgcatcc cgtcgggcca gctgatgccg
     2821 aggctgcggg cggcctgctg gaggcggggg aaggtggccg ggtcggcctg ggggacggtg
     2881 cggaggatcc cggttccggt ctcgatcatg agccaggccg ccgccctccc ggtgaggagc
     2941 gagatctggg cgttccagtg ttcgacgggc aggtcggccc ggagcttcac ggtccacgag
     3001 ccgtcacccg ccggtacgac ctcctgatcg gacagcccga gctcgatggc gccgcgctcg
     3061 cgggcggcgg cgaggagggc cgcgccgatg tcggggaagg cggcgatggc agcgggggcc
     3121 ttcccggcat cgagctggtc ctgcacctcg cggtaggtga gctgggcgcg gctgcgcacc
     3181 agcgcccgcc ggacgtcgac cgaggtcgtg acgccgccgc ggtcgacgcc gatggtccag
     3241 agcgctgcgg gccgagggcc gtccggcagc agcgacgcgg ccccctcgcc gagcacgggc
     3301 gggtgcagcg gcatccgcgc atcgggcgcg tagtaggtct gcgtccgctc ccgggcggcc
     3361 tggtcgaccg cgccgccggg atcgacccag gcggcgacgt cggcgatggc gtaggagacc
     3421 tgccacccgt cgtcggtggt ctcgatggcc acggcctggt cgagatcgcg cgagcccgga
     3481 gggtcgatgg tcacgaggtc gaggtgggtc gcgtcctcgt ggtcggggaa ccggcgcgcg
     3541 gcggcgtgat cggcttcggc gagcgcctcg gcgggaaagg cccccgggac atcgagctcc
     3601 tggcggatcg ccgcaaagtc gagcgggagg ggagccgacg aacggacgcg gtgggcaggc
     3661 acgtcgaagg ttctagcggg tggcgatcgg tagccttcgc ccatgtctga ctcgccggtg
     3721 gccgactacc ccgaccggtg ggagtccgag gtcgtgctgt ccgacggggg gacgatggcg
     3781 ctccggccga tccggcccga cgacgcggag cgcatcgtcg ccttccacga gcgccagtcg
     3841 cccgagagca tctacttccg gttcttctcg ccgcggccgc ggctgtcgga gcgggacctc
     3901 gagcggttca cccgggtcga ctacatcgac cgcatggcct tcgtcgggct gatcggcgac
     3961 gaactggtgg gtgtcgcccg ctacgaccgc caccgcgctc ggagcgatgc cgaggtcgcc
     4021 ttcttcatcg acgacgccca ccacggccgg ggcatggcga ccgtgctgct ggagtacctc
     4081 gccgaagcgg cccgggaggc gggcatctcg gggttcaccg cctcggtgct gccgcagaac
     4141 cgcaagatgc tcggagtctt cacccaggcc gggttcacgg cgtcgagccg cttcgaagac
     4201 ggcgtcatcg aggtccagct cagcatcgag ccgacgcccg cggccctggc cgccatcgag
     4261 gtgcgcgccg gcgtggccga agcccgatcg gtccagcgcc tgctgcggcc gacgtcgatc
     4321 gccgtcgtcg gtgcgtcgag gacgccgggc acgatcggcc accaggtgtt ccacgagctg
     4381 gtgagccgcg gcttccaggg acccgtgtac ccggtcaacc gggaggccga ctacgtcgcc
     4441 agcgtgcgct cgtggcggtc ggtcctcgac gtgccgggcg agctggacct ggccatcgtg
     4501 tgcgtgccgg ccggcgaggt cctgcgcgtg gccgaggagt gcgcctacaa gcgggtgcag
     4561 ggcctggtgg tgctgtcggc c
//
LOCUS       DATMFS010000033         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_187234, whole genome shotgun sequence.
ACCESSION   DATMFS010000033 DATMFS010000000
VERSION     DATMFS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187234"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VNQ33_00630"
     CDS             <1..996
                     /locus_tag="VNQ33_00630"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWJ96628.1"
                     /translation="GWIGRVVDGHIEIERENGTTDRLRATDLEPLLDHAPAQTTSWTT
                     QRAPRNPLRDREAGEQLLRNGWYRLPLLDADTVEALREGYGEVHGWTQAPTALGPDAF
                     YEPDPGNPDFEYRARSTALVSGMLESTMRPIFDDFEPFLWGFYCKWPHGEATALHWDW
                     TCVDSRLGHRSYQIWVALDDVDEANGGFAFLTGSHNLLSSPCGSGLSSPSSDTFLQRT
                     DDAIAKCESLRMRAGEALIFDHRILHITHPNTTDRPRLACVAAWRPAEADLVHYQRVS
                     DNVAIRYRADAWWFRNMVPGNLGSVGPSLELSEVVFLDDDLEAPADVGAFLDQVS"
     gene            complement(1091..2527)
                     /locus_tag="VNQ33_00635"
     CDS             complement(1091..2527)
                     /locus_tag="VNQ33_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWJ96629.1"
                     /translation="MTGSLRPRGDGGIAPTFRDPLLEERFLRDGFVVLPVLTEAELEA
                     CRRAWERVHPQPGSGFEYDVVRRKPEQTLALEAAIGATWDRVLDDTLVDHERFLLSFL
                     TKWPGDGSEHHLHQDWSYVDERRFRTMSYWIALDDTSAVLDNGPVAVIPGSQHLVDAY
                     RGPAVRDWYEDLAEDLTPQLEPVDVSAGHAIVIDNALLHGSSPNRSERPRRAVAAAVA
                     PRSAPYLFAAPADDDRVTVFELGQQYFAGFDPDAPEIEGRRRLGSAPRLVGVDHDGLG
                     SVVSSIPPDLAAAPGRAAVASDVASIEARAAHLGREHARLQADGWRPAPVRFLPDWAL
                     GLPPDVLVLPLAADGIPTDAGRVHREVAAALRLLPDVRRALVVTAPAGAVGTFGPCGG
                     TRIVVVLACLPAGSLEIGPPDRLRSVDTGTVLVGDRVDVRNHGRDAALLVIETGPSPR
                     GATRRARRLIRAADEAITATSPTARRYG"
     gene            complement(2524..3228)
                     /locus_tag="VNQ33_00640"
     CDS             complement(2524..3228)
                     /locus_tag="VNQ33_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWJ96630.1"
                     /translation="MGVDRFRRQGYERFDACFTRAEAAALLAAIGAVSSLDEDNDLSQ
                     GPMRFVSNVYRRSAEVQRFLLDPRILSIVGALVGPSAWCRWDQAVCKGPGAGTFPWHQ
                     DNGYTRLGREHLQIWVALTDAPAQRGGLLVEPGGHRSARSHRWVGNHVEVIDPPAEAL
                     ALDASAGDVIAFSSFLPHATGPNDTLDDRWAYVAEFLPLDAVDESVERPWFVLTSEGT
                     AVGRFLDQPPTRVASS"
     gene            3325..>3949
                     /locus_tag="VNQ33_00645"
     CDS             3325..>3949
                     /locus_tag="VNQ33_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ96631.1"
                     /translation="MQEEEQPGSIASVEAVGVPIHDPAWAPIGTDYAESTITRRFEER
                     VARAPESIAVTGPGGDLTASQLDRRANQVANAIREAAAPLGRPVAVLHGHDVDLVATM
                     LGVFKAGGILLVLDPASPPEVLASVVADARPAAIVTDTAHRPLGVELAGGDRPVVELD
                     EHLSSRSDDRPPLGTGPESPALLAYTSGTTGDVKAAVIPHRALLHLVR"
BASE COUNT          619 a         1375 c         1411 g          544 t
ORIGIN      
        1 ggttggatcg gtcgagtcgt cgacggccac atcgagatcg aacgtgagaa cgggacgacg
       61 gatcggctgc gcgcgacgga cctcgagccg ctgctcgatc acgcaccggc ccagaccacg
      121 agttggacga cgcaacgagc gccccgcaac cctctgcgcg accgggaggc cggcgagcag
      181 ctcctcagga acgggtggta ccgcctcccg ctcctcgatg ccgacaccgt cgaggccctc
      241 cgcgagggct acggcgaggt ccacgggtgg acccaagcac ccacggcgct gggaccggac
      301 gcgttctacg agcctgaccc gggcaacccg gacttcgagt atcgagcccg cagcacggcc
      361 ctggtgtcgg ggatgctcga gtcgaccatg cgaccgatct tcgacgactt cgagccgttc
      421 ttgtgggggt tctactgcaa gtggccccat ggcgaggcca cggcgctcca ctgggattgg
      481 acctgtgtcg actcgcgcct cgggcatcgg tcgtaccaga tctgggtcgc cttggacgac
      541 gtcgacgagg cgaacggagg gttcgcgttc ctcacgggca gccacaacct cctcagctcg
      601 ccgtgtggct cgggtctctc ctcgccgtcg agcgacacgt tcctccagcg caccgacgac
      661 gcgatcgcga agtgcgagag cctgcgcatg cgagctggcg aagccctcat cttcgatcac
      721 cgcatccttc acatcaccca ccccaacacc accgatcggc cccggctggc ttgcgtcgcg
      781 gcgtggcggc ccgcggaggc cgatctggtc cactaccagc gagtatcgga caacgtggcg
      841 atccgctatc gggccgacgc gtggtggttc cgaaacatgg ttccgggcaa cctcggatcg
      901 gtcggcccgt ctcttgagct cagcgaggtc gtcttcttgg acgacgacct cgaagctccg
      961 gcagacgtcg gcgcgttcct cgaccaggtg agctgagccc cggacgcgac ccgttgccgt
     1021 tccgctctga acgagggccc atcgtcacga cgaggcctca cgggtgacgg gctgatcccg
     1081 agcagcggat tcagccatac cgtcgggcgg tgggactcgt cgccgtgatc gcctcgtccg
     1141 ctgctcggat gaggcgtcga gcacgccggg tcgctcctcg tggggagggc ccggtctcga
     1201 tgacgaggag cgccgcgtcc ctgccgtggt tgcggacatc gacccgatcg ccgaccagga
     1261 ccgtgccggt gtcgacggac cggagccgat cggggggacc gatctcgagc gaaccggcgg
     1321 gaaggcacgc caagacgacg acgatgcgcg ttccgccgca cggcccgaag gtcccgacgg
     1381 caccggcggg tgcggtcacc acgagcgccc ggcgtacgtc gggaagcagg cggagggccg
     1441 cagcgacctc gcggtggacg cgccccgcgt ccgtcgggat gccgtcggcc gccaggggga
     1501 ggacgaggac gtccggaggg aggcccagag cccagtcagg caggaaacgg accggagcgg
     1561 gacgccaccc gtccgcctgc aggcgtgcgt gctcccgccc gaggtgggcg gcacgcgcct
     1621 cgatcgaggc cacgtcggag gcgacggcgg ctcgcccggg ggcggcagcg agatccggcg
     1681 ggatcgacga caccaccgag ccgaggccgt cgtggtcgac gccgacgaga cgaggggcgc
     1741 ttccgaggcg tcgtcggccc tcgatctcgg gggcatcggg gtcgaagccc gcgaagtact
     1801 gctggccgag ctcgaagacg gtcacccggt cgtcgtcggc gggagcagcg aacaggtagg
     1861 gcgctgatcg tggagcgacg gcggcggcga cggctcgtcg cggccgctcg gaccggttgg
     1921 gcgaggaccc gtgcaggagg gcgttgtcga tcacgatcgc gtgaccggcc gacacgtcga
     1981 ccggttcgag ctgcggcgtg agatcttcgg cgaggtcctc gtaccagtcg cgcacggctg
     2041 ggccccggta ggcatcgacg aggtgctggc tgccggggat caccgcaacc gggccgttgt
     2101 cgagcaccgc gctcgtgtcg tcgagggcga tccagtagga catggtgcgg aaccgccgct
     2161 cgtccacgta ggaccaatcc tggtggaggt ggtgttcgct tccgtcaccc ggccacttgg
     2221 tgaggaagct gagcaagaag cgctcatgat cgaccagcgt gtcgtccaag acccgatccc
     2281 aggtggcgcc gatcgccgcc tcgagggcga gggtctgctc gggtttcctg cgcaccacgt
     2341 cgtactcgaa gcccgaaccc ggctgggggt ggacgcgctc ccacgcccgt cggcaggctt
     2401 cgagctcggc ctcggtgagt acgggcagca ccacgaatcc atcgcggagg aaccgctcct
     2461 cgaggagcgg gtctcggaac gtgggtgcga tcccgccatc gccccgggga cgcaacgagc
     2521 ccgtcacgac gaagccaccc gagtcggggg ctggtcgagg aaccgaccca ccgcggtgcc
     2581 ctcgctggtg agcacgaacc acggccgttc gaccgactcg tccaccgcat cgaggggcag
     2641 gaactcggcg acgtaggccc accggtcgtc gagggtgtcg ttcgggccgg tggcatgggg
     2701 gaggaaggac gagaacgcga tcacgtcgcc ggctgacgcg tccagcgcca gagcctccgc
     2761 cggcgggtcg atgacctcca cgtggttgcc cacccaccgg tgggagcgtg cgctgcggtg
     2821 tccacccggc tccacgagca gaccgccgcg ctgcgccggg gcatcggtga gcgcgaccca
     2881 gatctggagg tgctcccggc cgagccgggt gtagccgttg tcctggtgcc aggggaaggt
     2941 gccggcgccg ggtcccttgc acaccgcttg gtcccaccgg caccaggcgc tcggtccgac
     3001 gagcgcaccc acgatggaca agatgcgcgg atcgaggagg aagcgctgga cctccgccga
     3061 acggcggtac acgttgctca cgaaccgcat cggaccctgc gagaggtcgt tgtcctcgtc
     3121 cagcgagctc accgcgccga tcgcggccag aagcgcggcg gcctccgctc gcgtgaagca
     3181 cgcgtcgaac cgttcgtagc cctggcggcg aaaccgatcc acacccacag cgaccaacgg
     3241 aaccttcctg accctcgacc ccgaaggctg gacgagacga tccccctatc atggcccgcg
     3301 tacctgagga ggtccgagcg agtgatgcag gaagaggaac agccgggatc gatcgccagc
     3361 gtcgaggcgg tcggcgttcc gatccacgac ccggcttggg cgccgatcgg gacggactac
     3421 gccgagagca ccatcacccg gcggttcgag gagcgcgtgg cccgtgcgcc ggagtcgatc
     3481 gcggtgacgg gtcctggcgg cgacctcacc gcatcgcagc tcgatcgccg ggcgaaccag
     3541 gtggccaacg cgatccgcga ggcggcggca ccgctgggcc ggccggtcgc ggtgctgcac
     3601 ggccacgacg tcgatctggt cgccacgatg ctcggggtgt tcaaagcggg cgggatcctg
     3661 ctcgtcttgg acccggcctc gccgccggag gtgctcgcct cggtcgtggc cgatgctcgc
     3721 cccgccgcga tcgtcaccga caccgcgcac cgccccctcg gcgtcgagtt ggccggcggc
     3781 gacaggccgg tcgtcgagct cgacgagcac ctgtcgagcc gatcggacga ccgaccgccg
     3841 ctcggcaccg gcccggagtc gccggcactg ctcgcctaca cgtcgggcac cacgggtgac
     3901 gtcaaggctg cggtcatccc ccaccgggcc ctgctccacc tggtccgcg
//
LOCUS       DATMFS010000034         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_94041, whole genome shotgun sequence.
ACCESSION   DATMFS010000034 DATMFS010000000
VERSION     DATMFS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94041"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VNQ33_00650"
     CDS             <1..861
                     /locus_tag="VNQ33_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96632.1"
                     /translation="VDGVDRPWRIDAGLARSLSEAIGWRADDGVAALAALMPVAVPMG
                     STAKLAAIAAGAVPPGEDPASLARQVLDHLEARAAGPVGGAPSPSWSCWVAATVMAAL
                     VDAVGIGPVHVASTRRSDEGAPVVDFHAAVQVSGGVDGGTWICDPYFGAALVLPAVPG
                     AAASVTGPLGTASAVRSGDGGWVFDLGWDVWDTVLRFRLLGPALDPGDVRAMAAVSVT
                     LSGVPLRPYARVHQHRSDGVAIADASENADGTGRLATWTRAAGRTVEEVPSWADAVEV
                     FAERTGVRVA"
     gene            1390..>2010
                     /locus_tag="VNQ33_00655"
     CDS             1390..>2010
                     /locus_tag="VNQ33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWJ96633.1"
                     /translation="MTRQIKLRPWQKAAFEKYVAAGQADFLTVATPGAGKTTFALTVA
                     RHVLSERPRARLIVVAPTAHLKVQWAQAAARFALHLDPAWSAADGALPADMHGIVTTY
                     QQVATSAEVLRPLATDAFVIFDEIHHAADDRAWGDSVRRAFETSARRLAISGTPFRSD
                     TQSIPFIDYVLEEAAPDYEYGYGDALGDGRVVRPVYFPRTGGEMEWT"
BASE COUNT          229 a          677 c          804 g          300 t
ORIGIN      
        1 gtggatggcg tggatcgacc gtggcgaatc gatgcggggc tggcccggtc gctctcggag
       61 gcgatcgggt ggcgggccga cgacggggtg gcggcgctcg ccgcgctgat gccggtggcc
      121 gtgccgatgg ggtcgacggc gaagcttgcg gccatcgccg ctggagcggt gccgccgggc
      181 gaggatcccg cgtcgctggc gcgccaggtg ctcgaccacc tcgaggctcg ggccgccggt
      241 cccgtcggtg gggcgccgtc gccctcgtgg tcgtgctggg tggcggccac ggtgatggcg
      301 gcgctggtcg atgctgtggg aatcggcccg gtgcacgtcg cctcgacccg gcggagcgat
      361 gagggcgcgc ccgtggtgga cttccacgcc gcggtgcagg tctcgggcgg ggtcgacggc
      421 gggacgtgga tctgcgaccc gtacttcggt gcggccctcg tgctgccggc cgtgccgggt
      481 gcggcggcgt cggtgaccgg gccgctcggg acggcgtcgg cggtgcggtc gggcgacggt
      541 ggctgggtgt tcgatctggg ctgggacgtg tgggacaccg tcttgcggtt ccggttgctc
      601 ggaccggcgc tcgaccccgg agatgtgcgg gccatggctg ccgtgtcggt gacgctgtcc
      661 ggtgtcccgc tccggcccta cgcccgggtg caccagcacc gcagcgacgg ggtcgccatc
      721 gccgacgcca gcgagaacgc cgacggcacc ggccggctcg ccacttggac ccgagcagcg
      781 gggcgcaccg tcgaggaggt cccgtcgtgg gccgacgccg tcgaggtgtt cgccgaacgg
      841 acgggcgtgc gggtggcctg atcctgagcg cgagcgcgag cccgagcccg acgggtctcc
      901 accgcagcgg tggtcaccgc gcgcgacatg gccggggggg ggtcggaagc ggtgggtggg
      961 catgccgtgc gctcacgccg caggcgaggg tcggggcgag cttccagcag gcgagcggcc
     1021 atcctccgag cgttcggtct cctcgtcgtg cgcagaccgt gcgggtgcct gagcccgagc
     1081 ctgatgggtc tccaccgcag cggtggtcgc cgcgcgcggg atggcggggg cgactgggaa
     1141 gcggtgggtg ggcatgccgt gcgctcacgg cggcgatccc cgagcgttcg gtctcctcgt
     1201 cgtgcgtaga ccgtccgggc ggccgtgggt gaccgccttg gcgaggggcg actcggaggg
     1261 ggcgaagatg accgagggcg tcgggttgct gaacggcatc gccgctggtc agaggtcatg
     1321 gatggcaccg gctgacgggc gtggcacact gccccttccc gtgttcgctc cgcccttccc
     1381 ccgctccctg tgacccggca gatcaagctc cgaccgtggc agaaggctgc gttcgagaag
     1441 tacgtcgctg ccggtcaggc cgacttcttg accgttgcca ccccgggcgc cggcaagacc
     1501 acgttcgcct tgacggtggc ccgccacgtg ctgtccgaac ggccccgggc ccggctgatc
     1561 gtcgtggcgc cgaccgccca cctgaaggtc cagtgggcgc aggctgcagc gcggttcgcg
     1621 ctgcacctgg acccggcgtg gtccgccgct gacggcgcgc tcccagcgga catgcacggg
     1681 atcgtgacca cgtaccagca ggtggccacc agcgccgagg tgctgcggcc gctggccacc
     1741 gatgccttcg tgatcttcga cgagatccac cacgccgctg acgaccgggc ctggggcgac
     1801 tccgtgcgcc gggcgttcga gacgtcggct cggcggctgg ccatctccgg cacgccgttc
     1861 cgcagcgaca cccagtcgat cccgttcatc gactacgtcc tggaggaggc ggcaccggac
     1921 tacgagtacg ggtacggcga cgccctcggc gacgggcggg tcgtccggcc cgtgtacttc
     1981 ccgcgcacgg gaggcgagat ggagtggacg
//
LOCUS       DATMFS010000035         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_256716, whole genome shotgun sequence.
ACCESSION   DATMFS010000035 DATMFS010000000
VERSION     DATMFS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256716"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNQ33_00660"
     CDS             <1..496
                     /locus_tag="VNQ33_00660"
                     /inference="COORDINATES: protein motif:HMM:NF033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HWJ96634.1"
                     /translation="DRRTRLREEAQRVEESAMYSAQSQFEYSKTWRRVDRWLGGAAAV
                     LAAASAAGGLSEVLSAQWAGVIALAAAGTAAVAASLGAPKAKDRAFSSANALLALQQD
                     ARIFINLDLDELSDDDAREQLRTLVARQQELNATAEIPSGRAWKRSKANIESGTQTYK
                     ADQK"
     gene            493..636
                     /locus_tag="VNQ33_00665"
     CDS             493..636
                     /locus_tag="VNQ33_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96635.1"
                     /translation="MTTTAQAFANFVSTISLTDIQRTEVSNKKTKTGEYLREAFPCRR
                     TCR"
     gene            633..1361
                     /locus_tag="VNQ33_00670"
     CDS             633..1361
                     /locus_tag="VNQ33_00670"
                     /inference="COORDINATES: protein motif:HMM:NF037736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96636.1"
                     /translation="MKRTILIGSADRGTLIRSLDDVDVMAEFTNKDNIFEQYRHNSGA
                     FLQRIRTALQASTSIASIGARGQAVRLFYTSGAHVDIAPTFKWSSGGFALPSGEGGWI
                     TTDPEVQATWMAGRREVLGSHLTPIVKIAKRWNRVHSSRLSSYHVEVMTATMFGSLSG
                     NYRDAMKCWFDWAPQYLDVNDPAGHSGSLGTSMTYATRTALKSRLAEAKTRAVKALAE
                     EAAGNHTEAKRLWKIELGDEFYTT"
     gene            complement(1429..1581)
                     /locus_tag="VNQ33_00675"
     CDS             complement(1429..1581)
                     /locus_tag="VNQ33_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96637.1"
                     /translation="MVEPEQDPTASIGEDRCQDGTVELIEVLVSEHQPYPETPGLHER
                     IRQIER"
     gene            1919..>2486
                     /locus_tag="VNQ33_00680"
     CDS             1919..>2486
                     /locus_tag="VNQ33_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HWJ96638.1"
                     /translation="MFASLFASARRTNRTTTCTAAASGRIVLRNVSLNREQTACHQGR
                     TDDRLVSTRPQHPRRPVPLRVLTGRQIECRWFRTPDVSRIGATRRTQRVLRRLHEQRR
                     IERLARRHGGVRGGSGSFTYWLAPLGRRAIGATGRGTFRPPSERFVDHCIAVAEIAVG
                     LHEAQEDGRICDLLVATEPDNWRRFQTAS"
BASE COUNT          425 a          862 c          814 g          385 t
ORIGIN      
        1 cgatcggcga acacgtctcc gtgaggaggc gcaacgggtt gaggagagcg cgatgtacag
       61 cgctcagagc cagttcgagt actcgaagac ctggcggcgg gtggatcgct ggctcggcgg
      121 cgccgccgca gtgctcgccg cggcgtccgc tgctggtggg ctgtcggagg tgctgtctgc
      181 gcagtgggcc ggggtgatcg ctctcgctgc cgcgggtacg gctgcggtgg cggcgtcgct
      241 gggggctccc aaggcgaagg accgggcgtt ctcgtctgcg aacgccttgt tggcgttgca
      301 gcaagacgca cggatcttca tcaacttgga tctcgacgag ctgtccgatg acgacgcccg
      361 cgagcagctg cgcaccctgg tcgcccgtca acaagagctc aacgccaccg ccgagatccc
      421 gtcaggacgg gcgtggaagc gcagcaaggc caacatcgag tccggcaccc agacctacaa
      481 ggcggatcag aagtgaccac gacagcccaa gcgttcgcga acttcgtgtc gacgatcagc
      541 ctcaccgaca tccagcgcac cgaggtctcc aacaagaaga cgaagaccgg cgagtacctg
      601 cgcgaggcgt ttccctgtcg tcgaacgtgc cggtgaaacg gacgatcctg atcggttccg
      661 cggaccgggg caccctgatc cggtctcttg atgacgtcga cgtgatggcc gagttcacga
      721 acaaggacaa catcttcgag cagtaccgcc acaacagcgg cgcgttcctc caacgcatcc
      781 gcactgccct ccaagcgagc acttcgatcg cgagcatcgg cgctcgaggc caggccgtgc
      841 ggctattcta cacgtcgggt gcgcacgtcg acatcgcgcc caccttcaag tggtcgagtg
      901 gcgggttcgc gctgccgtcg ggtgagggcg gctggatcac caccgatccc gaggtgcagg
      961 cgacgtggat ggccgggcgg cgcgaggtcc tcggttcgca cctgaccccg atcgtgaaga
     1021 tcgccaagcg gtggaaccgg gtccactcca gccgcctcag ctcgtaccac gtcgaggtca
     1081 tgaccgccac gatgtttggg tcgctgtcgg ggaactaccg cgacgcgatg aagtgctggt
     1141 tcgactgggc accgcagtac ctcgatgtca acgaccccgc cggccactcc ggttcgctcg
     1201 gcacgtcgat gacctacgcc acccgcaccg ccctcaagtc gcgactcgcc gaagccaaga
     1261 cccgcgccgt caaggccctg gctgaggaag ccgccgggaa ccacaccgaa gccaaacggc
     1321 tgtggaagat cgaactcggc gacgagttct acaccacctg accgcccgcc aggcgtagcc
     1381 tctcgggcgg cttgaggccc ccgacatccg cccggtcggg ggcctcgctt accgctcgat
     1441 ctggcggatg cgctcgtgca ggcccggggt ctcggggtag ggctggtgct cgctcaccag
     1501 cacctcgatc agctcgaccg tcccgtcctg gcagcggtcc tcgccaatgc tggcagtcgg
     1561 gtcctgttcc ggctcgacca cgacgacgcc accgtcatgg ccaagcgcac aggcggtctg
     1621 ctgaagcccg agcacctcca ggcgctcgac ccgtacgaga cctacacgtc ccataatgcg
     1681 gagtggtgaa ccgacccgct tcggctcggt cagaacgctg ccgctctacc catcgctgcg
     1741 agaccctggc gaactgctgg ctggcaaccg gacccgctgg ggcatccccg ccatcgacac
     1801 tgaggcgagg ctgcgagacc tcatcgacgg cggatcggac cctgctgctc gtggaccggt
     1861 cgggggccgg cgcaagctgc cagctgatac cggaggtccg ccgtgacggg ggtaggtggt
     1921 gttcgcgtcg ctgttcgcgt cggcgcgtcg aaccaaccgg acaaccacct gcacagcggc
     1981 agcttcgggt cggatcgtgc tgcggaatgt ctcgctcaac cgagagcaga ccgcctgcca
     2041 tcaaggccgt accgatgaca gacttgtctc cacgcgacct cagcatcctc gaagacctgt
     2101 cccgcttcgg gttctgaccg gacgccagat cgaatgccgc tggttccgca cccccgacgt
     2161 cagccggatc ggggccaccc gccgcaccca acgcgtcctg cgccgccttc acgagcagcg
     2221 tcggatcgaa cgccttgccc gccgccacgg tggggtccgg ggcggatcgg gcagcttcac
     2281 ctactggctc gcaccgctcg gccgtcgggc catcggggcg actggacggg gcaccttccg
     2341 gccaccgtcg gagcggttcg tcgaccactg catcgccgtc gcggaaatcg ccgtcggcct
     2401 ccacgaagcg caggaggacg ggcggatctg cgaccttctg gtagcgacgg aaccggacaa
     2461 ttggcggcgg ttccagaccg cgtcgg
//
LOCUS       DATMFS010000036         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_256718, whole genome shotgun sequence.
ACCESSION   DATMFS010000036 DATMFS010000000
VERSION     DATMFS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256718"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1203
                     /locus_tag="VNQ33_00685"
     CDS             307..1203
                     /locus_tag="VNQ33_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96639.1"
                     /translation="MQQRTVDLPFPIDLFRTFHGIRRGRHDPTCRFDGSGIWWATRRP
                     AGPATLHLVPHGDQVEATAWGQGAEATLDAVPSLIGCHDDSDDFDPQHPLVERLWRDL
                     AAVRIPRSGAVTETLVNVVLEQRVTTFEARRAQSQLVTRWSEPAPGPGDLLLPIDPEV
                     LAGIAYYDLHVIGVERARADTVRRVAASAGRLEALAVLPPAEAHQRLTGIVGVGPWSA
                     AQVALSALGDADAVPVGDVHLPSIVTFALTGEAVDSDDAMLEVLEPFAGHRGRVIRLI
                     GAAGISAPRHGPRYAPRDIRDQ"
     gene            1320..1559
                     /locus_tag="VNQ33_00690"
     CDS             1320..1559
                     /locus_tag="VNQ33_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96640.1"
                     /translation="MSRYRVTLMDRSTEEIDDVDAYQQEGQMTTFFRTGSDRQVVDTW
                     STRMASFRTSEIVAIRRLDPVAAASVAPVADLRSA"
     gene            1569..1772
                     /locus_tag="VNQ33_00695"
     CDS             1569..1772
                     /locus_tag="VNQ33_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96641.1"
                     /translation="MSIAEPVPETQTFTCPRCQSAVTEAYYGPCDPCRAELRAVQAGE
                     ARDVGVDYVPKMNVTPNAVASKD"
     gene            complement(1773..>2211)
                     /locus_tag="VNQ33_00700"
     CDS             complement(1773..>2211)
                     /locus_tag="VNQ33_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96642.1"
                     /translation="FGGYRNVTVDRSDARRSAEAIEARADDGALVVYCPDQLGPSTSR
                     ILGDRYDQVTYPRFDAPQLIDWSDYKERLDEVSVDDFATDLLERAEGRQIFLVYSTDY
                     ITHEETCPALFNAIGAQRSPEILTDTTEAWEPSAVVLFAPAGA"
BASE COUNT          300 a          838 c          775 g          298 t
ORIGIN      
        1 gcccagcacc tggcccgcta caagctgccc aaggacgtgg tggtggtcga gcgcatctcc
       61 cgttcgccgt cgggcaagcc cgactacgcg tgggcccggg accaggtgat ggggccgaac
      121 ggctagcgct gcgtcccttc gacccggtgg ggcggcgccc caccgggcgg cggcgaccgt
      181 ggcgcggttc ggggccccgg cacccgcccg tccgtcccgg cctccccggg agggtggcgg
      241 tccccccgcg gcggccgcta cggtcgactc cccgacggaa cacgtgttcg ccgggctccc
      301 cccaccatgc agcagcgcac cgtcgacctt cccttcccca tcgatctctt ccgtacgttc
      361 cacgggatcc gcaggggccg gcacgacccg acctgccggt tcgacggctc ggggatctgg
      421 tgggccacgc gccgtcccgc cgggccggcc acgctccacc tggtgcccca cggcgaccag
      481 gtcgaggcca cggcgtgggg ccaaggcgcg gaagcgaccc tcgacgccgt gcccagcctc
      541 atcggctgcc acgacgacag cgacgacttc gacccgcagc acccgctggt ggagcgcctg
      601 tggcgggacc tcgccgccgt gcgcatcccg cggtcgggtg cggtgaccga gaccctcgtc
      661 aacgtggtcc tcgagcagcg ggtcacgacg tttgaagccc ggcgggccca gtctcagctg
      721 gtgacgcgct ggagcgagcc ggcgcccggc ccgggcgatc tcctgctgcc gatcgacccc
      781 gaggtcctcg ctggcatcgc ctactacgac ctccacgtca tcggcgtgga gcgggcccga
      841 gccgacaccg tccgccgcgt cgcggcgtcg gccggtcgcc tggaggcgct ggccgtgctg
      901 ccgccggccg aggcgcacca gcggctcacc ggcatcgtcg gcgtcgggcc gtggagcgcc
      961 gcccaggtgg cgctgtccgc cctcggcgac gccgacgccg ttccggtggg cgacgtccac
     1021 ctcccgtcga tcgtcacgtt cgccctcacc ggtgaggcgg tcgacagcga cgacgccatg
     1081 ctcgaggtcc tggagccgtt cgccgggcac cggggccggg tgatccgcct catcggcgcc
     1141 gccggcatct cggcgccgcg ccacggcccg cgctacgccc cccgagacat ccgcgaccag
     1201 tagccgcgcc gcgaggggcg ggcctgtgga caacttcgcc aatccacagg gttgctcccc
     1261 ttcctcgtca cagggccgcc gtcgtaccgt ggtctcagcg cccgacgaag gaggtcacca
     1321 tgtcccgcta ccgcgtcacc ctcatggacc gcagcaccga ggagatcgac gacgtcgacg
     1381 cctaccagca ggagggccag atgacgacgt tcttccgcac cgggtcagac cggcaggtcg
     1441 tcgacacgtg gagcacgcgg atggcgagct tccgcacctc ggagatcgtc gccatccggc
     1501 ggctcgatcc ggtggcggcg gcatcggtcg caccggtcgc cgacctccgc tccgcgtagc
     1561 ctggggccat gtcgatcgcc gagccggtcc cggagaccca gacgttcacc tgcccccgct
     1621 gccagagcgc cgtcaccgag gcgtactacg gcccctgcga cccgtgccgc gccgagctcc
     1681 gcgccgtgca ggcgggcgag gcccgcgacg tcggcgtcga ctacgtcccg aagatgaacg
     1741 tgaccccgaa cgccgtcgcc agcaaggact gatcaggccc cggccggggc gaacaagacg
     1801 acggccgacg gctcccaggc ctcggtcgtg tcggtgagga tctccggcga ccgctgggcg
     1861 ccgatggcgt tgaacagcgc cgggcaggtc tcctcgtggg tgatgtagtc ggtgctgtac
     1921 acgaggaaga tctgacggcc ttccgcccgc tccagcaggt cggtggcgaa gtcgtcgacc
     1981 gagacctcgt cgagccgctc cttgtagtcg gaccagtcga tgagctgggg cgcatcgaag
     2041 cgcgggtagg tgacctggtc gtaccggtcc cccaagatgc gcgaggtcga cgggccgagc
     2101 tggtccgggc agtagacgac cagcgcgccg tcatcggcac gagcctcgat ggcctcggcc
     2161 gaccggcggg catcggagcg gtcgacggtg acgttgcggt agccgccgaa c
//
LOCUS       DATMFS010000037         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_163781, whole genome shotgun sequence.
ACCESSION   DATMFS010000037 DATMFS010000000
VERSION     DATMFS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163781"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VNQ33_00705"
     CDS             <1..405
                     /locus_tag="VNQ33_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWJ96643.1"
                     /translation="VRSLTGAFVLDGGQLTDGVELVVDCVGSEASLSQALQVVAPGGQ
                     ILVVGMPGHTTLDLTGLWHRESSISGCYAYTRSDFERSLELAHEHDLGRLVSATYPLS
                     RYQEAIEHAANAGLRGAVKVAFDLRKPAKAKR"
     gene            530..2122
                     /locus_tag="VNQ33_00710"
     CDS             530..2122
                     /locus_tag="VNQ33_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWJ96644.1"
                     /translation="MPRPGFVLDVDKSTPPILFHHGEGFRLEKLPADRSRVIYPGEPL
                     DALDDVEQSIQDALLNPLDMDPLPTLLKPGMKLTIAFDDVSLPLPPMKRPDIRQRVIE
                     AVLDMAAAAGVDDVHLIAALALHRRMTEDELRHAVGDRVYDAFAPQGALSNHDAEDPD
                     NLAFLGETKHGEEVEINKRAAESDLLVYVNINIVSMDGGHKSTATGLASYRSIKHHHN
                     VHTMEHSKSFMDRHNSELHHSNWRMGKVIADAGVKVFQIETTINNDTFGDALPMSVLA
                     KREWEWTAKDRVSFIGMQQGLKRLSGPTKRKIFQAHRAPYGVTSVTAGAIEPVHEKTL
                     RHCFDQHIVEVEGQTDILTMGLPYICPYNVNSIMNPILVMCLGLGYFFNMYRGKPLVR
                     EGGVLIMTHPTPWEFHPVHHPSYIDFFEQVLADTTDPLEIEAKYEKQYAEDEWYRHLY
                     RTSYAYHGVHPFYMWYWGSHALKHLGRVIVVGGDKRAVHRLGFKAASTLNDAFEMAED
                     VVGRHPTITHLKNPPIVMADVK"
     gene            2133..>2426
                     /locus_tag="VNQ33_00715"
     CDS             2133..>2426
                     /locus_tag="VNQ33_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96645.1"
                     /translation="MSDKKGLEKLAASVQKARSKVPPVRAVVAQTSFPYRAPTTPLTV
                     EPKVEKPTLLGHYDSDWARRLPARYARFFLTEGLVRPVIAGLAQPERKGLDRLA"
BASE COUNT          438 a          908 c          732 g          348 t
ORIGIN      
        1 gtccgcagcc tcaccggtgc gttcgtgctc gacggtggcc agctcaccga tggcgtggag
       61 ctcgtggtcg actgcgtcgg cagcgaggcc agcctcagcc aggccctcca ggtggtggcc
      121 cccggcggac agatcctggt cgtcggcatg cccggccaca ccacgctcga cctcaccggc
      181 ctctggcacc gcgagagcag catcagcggc tgctacgcct acacccggtc ggacttcgaa
      241 cggtccctcg agctcgccca cgagcacgac ctcggccgcc tcgtgagcgc gacgtacccc
      301 ctgagccgct accaggaggc catcgagcac gccgcgaacg ccggcctgcg cggcgccgtc
      361 aaggtcgcct tcgacctccg caagcccgcc aaggccaagc gatgaccccc gccgccctcc
      421 accccgtccc aacttttcgt gaagttggtg gctcagccac cacgatcacg aaaagttcgg
      481 atggtccgcc actgagcccc tcccctgctc tgaacgagga agcctcctga tgccccgtcc
      541 cggtttcgtg ctcgacgtcg acaagtcgac gccgcccatc ctgttccacc acggtgaggg
      601 cttccgcttg gagaagctcc ccgccgaccg gtcgcgggtg atctaccccg gagagccgct
      661 cgacgcgctc gacgacgtcg agcagagcat ccaagacgcc ctcctgaacc cgctcgacat
      721 ggatccgctg ccgacactgc tgaagccggg catgaagctg accatcgcgt tcgacgacgt
      781 gtcgctgccc cttccgccca tgaagcggcc cgacatccgc cagcgggtca tcgaggccgt
      841 cctcgacatg gccgccgccg ccggcgtcga cgacgtccac ctcatcgcgg cgctcgccct
      901 gcaccgccgc atgaccgaag acgagctgcg ccacgccgtc ggcgaccggg tctacgacgc
      961 cttcgccccg cagggcgccc tctccaacca cgacgccgaa gaccccgaca acctggcgtt
     1021 cctgggcgag accaagcacg gcgaggaggt cgagatcaac aagcgggccg ccgagtccga
     1081 cctgctggtg tacgtcaaca tcaacatcgt ctcgatggac ggcggccaca agagcaccgc
     1141 caccggcctg gccagctacc gctccatcaa gcaccaccac aacgtgcaca ccatggagca
     1201 ctccaagagc ttcatggacc gccacaactc cgagctgcac cacagcaact ggcgcatggg
     1261 caaggtcatc gccgacgccg gggtgaaggt cttccagatc gagaccacga tcaacaacga
     1321 caccttcggc gatgccctcc cgatgtccgt gctggccaag cgcgagtggg agtggacggc
     1381 gaaggaccgg gtctcgttca tcggcatgca gcaggggctc aagcgcctgt cgggcccgac
     1441 caagcgcaag atcttccagg cccaccgggc gccctacggc gtcacctcgg tgaccgcggg
     1501 cgccatcgag ccggtgcacg agaagacgct ccggcactgc ttcgaccagc acatcgtgga
     1561 ggtcgagggc cagaccgaca tcctcaccat ggggctcccc tacatctgcc cctacaacgt
     1621 gaactcgatc atgaacccga tcctggtcat gtgcctggga ctcgggtact tcttcaacat
     1681 gtaccgcggg aagccgctgg tccgcgaggg cggcgtgctg atcatgacgc accccacgcc
     1741 gtgggagttc cacccggtcc accacccgag ctacatcgac ttcttcgagc aggtcctggc
     1801 cgacaccacc gacccgctgg agatcgaggc caagtacgag aagcagtacg ccgaggacga
     1861 gtggtaccgc cacctgtacc ggacgagcta cgcgtaccac ggcgtccacc ccttctacat
     1921 gtggtactgg ggctcgcacg cgctgaagca cctcggacgg gtgatcgtcg tcggcggcga
     1981 caagcgcgcc gtccaccgcc tgggcttcaa ggcggcgtcg acgctgaacg acgccttcga
     2041 gatggccgaa gacgtcgtcg gccgccaccc caccatcacg cacctgaaga acccgcccat
     2101 cgtcatggcc gacgtgaagt gacggtgcgc tgatgagcga caagaagggg ctggagaagc
     2161 tcgctgcttc ggtgcagaag gctcgttcca aggtgccgcc ggttcgggcc gtcgtggccc
     2221 agacgtcgtt tccgtaccgg gcacccacca caccgctcac ggtcgagccg aaggtcgaga
     2281 agccgacgct gctcgggcac tacgactcgg actgggcccg caggctgccc gcccggtacg
     2341 cccgcttctt cctcaccgaa gggctggtgc ggcccgtcat cgccgggctg gcccagcccg
     2401 agcgcaaggg cctcgaccgc ctggcc
//
LOCUS       DATMFS010000038         4273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_233866, whole genome shotgun sequence.
ACCESSION   DATMFS010000038 DATMFS010000000
VERSION     DATMFS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233866"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VNQ33_00720"
     CDS             <1..719
                     /locus_tag="VNQ33_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWJ96646.1"
                     /translation="DYEGMVALMLQPAVVGRYEIDLATGEVLGAETAMDADQVWGGIL
                     PTTDTSTPAARNRQRQLWYAAAGFDPDLIPEEWWRLYGDATDGAVAPADLPSDAVPGT
                     LARIDLESMKVAEVWTYADGAFPSPPTFVPRVGAEDPGDGYVVVVVHQDGDKELQVFD
                     ALHIEAGPLARATAPGFNPGLLLHSCWMADRVGPRPSSYRVTHGADVRGAIRGLPGVV
                     ASVFRMGKAIAEQERAARKP"
     gene            972..1436
                     /locus_tag="VNQ33_00725"
     CDS             972..1436
                     /locus_tag="VNQ33_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96647.1"
                     /translation="MARASSLQDIGDADGWRCWLCDEPVDPDMSVNDPRGPSVDTRIT
                     KAKAKAKAKSGAAGPDRLAHRGCNTGKGATSPEVPWSDDLFVVDPAPILASVERLVRK
                     GGREVMARCPTESDAEAAADWLVHRISRLEPGLAVTAEIGPGGGQYLVALRA"
     gene            1569..1802
                     /locus_tag="VNQ33_00730"
     CDS             1569..1802
                     /locus_tag="VNQ33_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96648.1"
                     /translation="MGAITVRDVPDETRDELAARAARSGRSLQEYLRLELIELARRPE
                     MEAVLARVRQRKRATGTKFSAEEIIELRDADRR"
     gene            1808..2197
                     /locus_tag="VNQ33_00735"
     CDS             1808..2197
                     /locus_tag="VNQ33_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ96649.1"
                     /translation="MADASVVVAWLVDDGDDGRWAEEVLAIGAIAAPHLMPAEVASTL
                     RRRVLSGRLAPEIAALALEDLQQASFDLFPYEPFAQRVWELRETVTPYDAWYVAMGEA
                     LDAPVASLDARLTRAPGPRCRFLTPVD"
     gene            2768..3991
                     /locus_tag="VNQ33_00740"
     CDS             2768..3991
                     /locus_tag="VNQ33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ96650.1"
                     /translation="MVDHERTVYGGVDTHKEFHVAAVVDEIGRILGTETFPATAAGYR
                     RLARWLDSHGTIAKVGVEGTGAYGLGLARFLTARGIEVVEVNRPNRQLRRRRGKSDTV
                     DAEAAARAVLNGEATSVPKAADGIVEAIRALRIVFCSTRNHRTRIANQIRDLLVTAPD
                     QLREALEPLDTDERVARAARFRCGEGSEPAETTKAALRTLARQHQMLTNDLDALRTRL
                     DELTTQANPALRNAVGIGPDVASILLVAAGDNPERLTSDAAFAALCGASPVEASSGKH
                     VGHRLNKGGNRDANHALWRIVMVRMTCHQPTKDYVARRRAQGKSQQFIIRCLKRYVAR
                     EIHQLLLHPQLATTGRDLREARRRAGHTQHDIADALSVAPARISEIENNRRCNHEIIN
                     RYRDWLNTHTTTCAA"
     gene            complement(4031..>4273)
                     /locus_tag="VNQ33_00745"
     CDS             complement(4031..>4273)
                     /locus_tag="VNQ33_00745"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HWJ96651.1"
                     /translation="ARSWHPDTTPRRIHDDPTRRWTGLDVLATTKGGMLDQEGTVEFE
                     AHHTGGAGDEPGDHVVHEVSTFTRVDNRWVYVGRLS"
BASE COUNT          669 a         1537 c         1471 g          596 t
ORIGIN      
        1 aggactacga gggcatggtg gcgctgatgc tccagcccgc cgtcgtcggc cggtacgaga
       61 tcgacctcgc gacgggcgag gtgctcggcg ccgagaccgc catggacgcc gaccaggtgt
      121 ggggcggcat cctgccgacg accgacacgt cgaccccggc cgcccgcaac cggcagcgcc
      181 agctctggta cgccgcggcg ggcttcgacc ccgacctcat cccggaggag tggtggcgcc
      241 tctacggcga cgccaccgac ggcgccgtcg ccccggccga cctgccctcg gatgcggtgc
      301 ccggcaccct ggcccgcatc gacctcgagt cgatgaaggt ggccgaggtg tggacctacg
      361 cggatggcgc cttcccgtcg ccgcccacgt tcgtgccccg cgtcggcgcc gaggatcccg
      421 gtgacgggta cgtcgtggtg gtcgtccacc aagacggcga caaggagctc caggtcttcg
      481 acgccctcca catcgaggcc gggccgctcg cccgggccac ggcccccggc ttcaatcccg
      541 gcctgctcct ccactcgtgc tggatggccg accgcgtcgg ccctcgcccc agctcgtacc
      601 gcgtgacgca cggcgccgac gtgcggggtg cgatccgcgg cctccccggc gtcgtcgcca
      661 gcgtcttccg catgggcaag gccatcgccg aacaagaacg agccgcccgc aagccctgac
      721 ggggggcgaa cgaatgttcg atggaaaaag gctgcggtgc aaccacgatc atcgaacgtt
      781 cggtggaggc gggttgctcg atcgggccga acgggctggt cgggcggggt gtcgtcgagg
      841 ctcaaccgga tctggcggag gagggaggag ggtcgcgagc tggtcctgct gctcgggctc
      901 gccaccacgc tcgccgccat cgcacggggc gccaccctcc ctgccgggga cgggccggta
      961 ccgtcgcctc catggcccga gcgagcagcc tccaggacat cggtgatgca gacggttggc
     1021 ggtgctggct ctgcgacgag cccgtcgatc ccgacatgtc cgtgaacgac ccccgaggtc
     1081 ccagcgtcga cacccggatc accaaggcga aggccaaggc caaggccaag tccggggccg
     1141 ccggccccga ccgcctggcc caccgtgggt gcaacaccgg caagggggcg acgagccctg
     1201 aggtgccgtg gtccgacgac ctgttcgtgg tggatccggc gccgatcctc gccagcgtcg
     1261 aacgcctggt ccgcaagggc ggccgcgagg tgatggcccg gtgccccacc gagtccgatg
     1321 ccgaggcggc ggcggactgg ctggtccacc gcatctcccg actcgagccc ggcctcgccg
     1381 tcaccgccga gatcggaccc gggggcggcc agtacctcgt cgccctccgt gcctgacgcc
     1441 tcgccggctc gccttgagcc agatcggtcg catccctggc gggacccgag acggaactgc
     1501 agcctggagg catggcctcc tcaaccaagg acgttgcaga tcgcaaccat tcgctgcaaa
     1561 atgcaggcat gggtgcgatc accgttcgag atgtgccgga tgaaacccgc gatgagctcg
     1621 ccgcacgagc ggctcgatcg ggtcgctcgc tccaggagta cctgcggctc gagctgatcg
     1681 agctggcgcg acggccggag atggaggcgg tgctggcccg cgtccgccag cggaagcggg
     1741 cgacgggcac gaagttctcg gcggaggaga tcatcgagct tcgagacgca gaccggcggt
     1801 gagcgtggtg gccgacgcat cggtcgtggt ggcgtggctc gtcgacgacg gcgacgacgg
     1861 gcgatgggcc gaggaggtcc tggccatcgg agcgattgcc gcaccccacc tcatgccggc
     1921 cgaggtggcg agcacgcttc ggcgcagggt gctcagcggg cgtctcgcac ccgagatcgc
     1981 agccctggcc ttggaggatc tgcagcaggc gtcgttcgac ctcttcccgt acgagccgtt
     2041 cgcccaacgg gtctgggagc tccgggagac cgtcacgccg tacgacgcgt ggtacgtcgc
     2101 gatgggtgaa gcgctcgacg cgcccgtcgc ttcgctcgac gcgcggttga cccgggctcc
     2161 ggggccccgc tgccggttcc tcacacccgt cgactgaacc gtcacggggg tcgagggcgc
     2221 gcggcaggcg agcccgtcgg gcagggtcgc atcccacgct tcccggtcgt cggtggaccg
     2281 ttcgcgggag gacgcgacgg acgtgtcccg atgctcggag ctctgcaacc ggtcggtggc
     2341 ctgatggatg acctcggatc gggagcggta gccgcgggtg ctcgcgaagc gatggatgaa
     2401 cgccacatgg cgctcatcga ccgcgatggt gatggcgagc ctcgtgggcg cacgtctgcc
     2461 cggctcgccg gggacggagg aacgaggggt gcggcacgca gggagggcat ggctcaaacc
     2521 tacgaacggg gtctgacact caagatggtg gggtccccgg tcggggtcga gtctgactct
     2581 gggtgcgact gacgatgtga tgtcttgtcg ctgatctgtc ggctcggtcc tggccgtgga
     2641 cgcgcaccag ccggtcgggt cgaacgtgac ctcataggag ctttgagcag tcaccctgag
     2701 tgtcctgtcc gaccgtcccg gccggtggtg cccttccaac catcgagcaa gaaggaccgg
     2761 atcaagcatg gtcgaccacg aacgcaccgt ctacggcggt gtcgacaccc acaaggagtt
     2821 ccacgtcgca gcagtcgtcg acgagatcgg acggatcctc ggcaccgaga cgttcccggc
     2881 gaccgcggcc ggctaccggc gcctggcgcg ttggctcgat tcgcacggca cgatcgcgaa
     2941 ggtcggcgtc gaggggaccg gcgcctacgg cctcggcctc gcccggttcc tcaccgcacg
     3001 cgggatcgag gtggtcgagg tcaaccgccc gaaccgtcag ctccggcggc gtcgaggcaa
     3061 gagcgacacc gtcgatgccg aggccgcggc gagggcggtg ctcaacggtg aggccacgtc
     3121 ggtcccgaag gccgctgacg ggatcgtcga agccatccgg gcgctgcgga tcgtgttctg
     3181 ctcgacgcgc aatcacagga cccgcatcgc caaccagatc cgcgacctgc tcgtcaccgc
     3241 ccctgaccag ctccgcgagg ccctcgaacc gctcgacacc gacgagcggg tcgcccgagc
     3301 cgcccggttc cgatgcggag agggctccga gcctgctgag acgacgaagg ctgcgctgcg
     3361 caccctcgcc cgccagcacc agatgctcac caacgacctc gacgccctgc gaacccggct
     3421 cgacgaactc accacgcagg ccaacccggc cctgcgcaac gccgtgggca tcggtcctga
     3481 tgtcgcgtcc atcctgctgg tcgccgccgg cgacaacccc gaacggctca ccagcgacgc
     3541 cgccttcgcc gccctctgcg gggcgtcacc ggtcgaggcc tcctcgggca aacacgtcgg
     3601 ccaccgcctc aacaagggcg gcaaccgcga cgccaaccac gccctctggc gcatcgtcat
     3661 ggtccgcatg acctgccacc aaccgaccaa ggactacgtc gctcgccggc gtgcccaagg
     3721 caagagccag cagttcatca tccgctgcct caagcgctac gtcgcccgcg agatccacca
     3781 actcctgctt catccccaac tcgccaccac cggccgtgac ctccgagaag cccggcgccg
     3841 cgccggccac acccagcacg acatcgccga cgctctcagc gtcgctcccg cccgcatcag
     3901 cgagatcgag aacaaccgac gctgcaacca cgagatcatc aaccgctacc gcgactggct
     3961 caacacccac accacgactt gcgcggcata ggagcatcag cgaagccccc ggtgaagccg
     4021 gcgtgcgacc tcagctgagg cggccgacgt agacccagcg gttgtcgacc ctcgtgaagg
     4081 tgctcacctc gtggaccacg tggtcgccgg gctcgtcgcc ggcgccgccg gtgtggtgcg
     4141 cctcgaactc caccgtgccc tcctggtcga gcatcccgcc cttggtggtg gcgaggacgt
     4201 cgaggccggt ccagcggcgg gtcgggtcgt cgtggatcct ccgaggcgtg gtgtcggggt
     4261 gccaggaccg ggc
//
LOCUS       DATMFS010000039         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_24734, whole genome shotgun sequence.
ACCESSION   DATMFS010000039 DATMFS010000000
VERSION     DATMFS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24734"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..2096
                     /locus_tag="VNQ33_00750"
     CDS             198..2096
                     /locus_tag="VNQ33_00750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWJ96652.1"
                     /translation="MTTTATAPPTSATNARARIGGLDGLRAVAVIGVLLYHGDVSWFP
                     GGFIGVDIFFVLSGYLVTTIVMDGLEKRGGLGFRRFWANRFRRLEPAQITMMVVVTLV
                     VAVGFRGLLGTIRAQVIAGLSGTMNWYLIVNNSSYFEQAARAPLFRHLWSLAIELQFY
                     LVWPLTLVALAKRYRDRVSVVVGGLAVAILASAVYMAILYHPGTDPSRPYFDTFARIQ
                     APLVGALLALLWRPRALRRARAGQHGGQVTAVAAVSLVLLIVMMHVVNDRDAFMYRGG
                     FLLTALLSALVIAGLVHPRGALGSELALGSSPMVAIGLRSYGLYLWHWPVFVLLRPRI
                     DTDWSWGTVFVVRLAITIALTEICYRLVERPWHTRAPDTNLAGIRRRLTQPAGYAPAP
                     RLSAVGSAVACALALVIIAVPHKADNVIVDSLTEGEAALANQSKGTIPGGGTTSVPAG
                     AGPTTTVPIVPEPGSPVSLVGDSVMVGAAPTVLEEFGNRAIIDAKVSRQAADITPVIE
                     RLKSEGQLGPTVVVQVGINGTVTDQNLRDIVGAAEGRRVLIINARVPRSWEAGNNELV
                     SELVPKLTNAGVIDWYSYSDGHRDWFLDDGVHLTEDGRKAYADLILEAVDGKEKRTGS
                     KSGGAKSD"
     gene            complement(2184..>3641)
                     /locus_tag="VNQ33_00755"
     CDS             complement(2184..>3641)
                     /locus_tag="VNQ33_00755"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ96653.1"
                     /translation="YLPLLEETGHVPSAKYTPAAENFAHARAIATQYGLYEHALFSTK
                     VTGLEWDGGASRWTVRTDRGDAFTAQFVAMGTGPLQKPKLPGIPGIETFAGHTFHTSR
                     WDYGYTGGDPTGAPLDRLADKRVGIIGTGATAVQCIPHLARGAGELLVFQRTPSSIDV
                     RSNHAIDPQWFGDLEPGWQRRWQMNFTILQTGGFTDEDLVEDGWTDISQRIRDRVVAE
                     LSKEGAEFTPELVARAFEDSDDEKMTEIRARVDEIVEDPATAANLKPWYRQLCKRPCF
                     HDEYLQAFNEPGTRLVDTDGQGVERIDETGVWVGGQHHELDCLIFASGFEFGADITRR
                     SGFETIGRDGEALSDHWAGGMRTLHGIHVHGFPNLFVVGITQAANLISNITHNLTEAG
                     TTVAAVVGHAVEIGADQVEVTADAEAAWLAQLDSTTGLFGNLDCTPGYYNNEGRAFSE
                     RDRLNGARYAAGPVAYFDHIDRWRRSGTFEGLVFR"
BASE COUNT          479 a         1287 c         1303 g          572 t
ORIGIN      
        1 cccgtcgcca tcaccgccat ggccgaccgc cagaccaccg gccgcgtgat cgcgatggtc
       61 gaggagggcc cgtgacggtc cggctgacgg tcctcgaacc ccggcggagc ggtgcccgtg
      121 cctgacgtgc tgcccagtgc ggatgcacgc ggcggaccag cacgatcggt accctcgccg
      181 ggagcaccga gacgaccgtg acgacgaccg cgaccgcacc accaacctcc gcaacgaacg
      241 ctcgggcacg catcggcggg ctcgacggtc tgcgcgccgt cgccgtgatc ggcgtgctgc
      301 tctaccacgg cgacgtcagc tggttccccg gcggcttcat cggcgtcgac atcttcttcg
      361 tgctgagcgg ctacctcgtc acgaccatcg tcatggacgg gctggagaag cggggcggcc
      421 tcggcttccg ccgcttctgg gcgaaccgct tccgacgcct cgagccggcc cagatcacga
      481 tgatggtggt cgtcacgctc gtggtggccg tcggcttccg cggcctgctg ggcacgatcc
      541 gcgcccaggt catcgcgggt ctctcgggca ccatgaactg gtacctgatc gtgaacaaca
      601 gctcgtactt cgagcaggcg gcccgggcac cgctgttccg ccacctgtgg tcgctcgcga
      661 tcgagctgca gttctacctg gtgtggcccc tcaccctcgt ggccctggcc aagcgctacc
      721 gagaccgcgt gagcgtggtg gtcggcgggc tcgccgtcgc catcctcgcg tctgccgtct
      781 acatggcgat cctgtaccac ccgggcaccg atccgtcccg gccgtacttc gacaccttcg
      841 cccgcatcca ggctccgctg gtgggcgccc tgctcgcgct gctctggcgg ccgcgcgccc
      901 tgcgccgggc gcgcgccggg cagcacggtg gccaggtcac ggccgtcgcc gccgtgtcgc
      961 tggtcctgct gatcgtgatg atgcacgtcg tcaacgaccg cgatgcgttc atgtaccggg
     1021 gcgggttctt gctcacggcg ctcctgtcgg cgctggtgat cgccggcctg gtgcacccga
     1081 ggggcgcgct cggcagcgag ctcgcgctgg ggagctcgcc gatggtcgcc atcgggctcc
     1141 gctcctacgg gctctacctg tggcactggc cggtcttcgt cctcctccgc ccccgcatcg
     1201 acaccgactg gtcgtggggg accgtgttcg tggtccgcct ggcgatcacg atcgccctga
     1261 ccgagatctg ctaccggctg gtcgagcgcc cgtggcacac ccgggcaccc gacacgaacc
     1321 tcgccggcat caggcgccgc ctcacccagc ccgccggcta cgccccggcg ccgcgcttgt
     1381 cggcggtcgg ctccgcggtc gcctgtgccc tggcgctcgt catcatcgcc gtgccccaca
     1441 aggccgacaa cgtgatcgtc gactccctca ccgagggcga ggcggcgctg gccaaccagt
     1501 ccaagggcac gatcccgggc ggcggcacga cctcggtccc ggccggtgcc gggccgacca
     1561 cgacggtgcc catcgtcccg gagccgggga gcccggtctc gctcgtgggc gactcggtga
     1621 tggtcggcgc cgcgccgacc gtcctcgagg agttcggcaa ccgggccatc atcgatgcca
     1681 aggtctcccg ccaggcggcc gacatcacgc ccgtcatcga gcggctcaag tccgaggggc
     1741 agctcggtcc cacggtggtg gtgcaggtcg gcatcaacgg caccgtcacc gaccagaacc
     1801 tccgggacat cgtcggcgcc gccgagggtc gccgggtgct catcatcaac gcccgggtcc
     1861 cgcgctcgtg ggaggccggc aacaacgagc tggtcagcga gctggtcccc aagctcacga
     1921 acgcgggcgt catcgactgg tactcctact ccgacggcca ccgcgactgg ttcctcgacg
     1981 acggcgtcca cctcaccgag gacggccgca aggcctacgc cgacctcatc ctcgaggcgg
     2041 tcgacggcaa ggagaagcgc accgggtcga agagcggcgg cgccaagagc gactgatccc
     2101 tggcgccgcg gcacctcccc acctcaggct gacccgggct cgtgcgggcc tgatctcacg
     2161 cggggcgcgg gtctggtcag cggtcaccgg aacaccaggc cttcgaaggt gccggagcgc
     2221 cgccagcggt cgatgtggtc gaagtacgcc acgggcccgg cggcgtagcg ggcaccgttg
     2281 agccggtctc gctcggagaa cgcccggccc tcgttgttgt agtagccggg cgtgcagtcg
     2341 aggttgccga acaggccggt ggtgctgtcg agctgggcca gccacgccgc ctcggcgtcg
     2401 gcggtgacct ccacctggtc ggcgccgatc tcgacggcgt ggccgaccac ggcggcgacg
     2461 gtggtcccgg cctcggtgag gttgtgggtg atgttcgaga tgaggttggc ggcctgggtg
     2521 atgccgacga cgaacaggtt cgggaacccg tggacgtgga tgccgtggag cgtgcgcatg
     2581 ccgccggccc agtgatccga gagcgcctcg ccgtcgcggc cgatggtctc gaaccccgac
     2641 cgccgcgtga tgtcggcccc gaactcgaaa cccgaggcga agatcaggca gtcgagctcg
     2701 tggtgctggc cgccgaccca cacgcccgtc tcgtcgatgc gctcgacccc ttggccgtcg
     2761 gtgtcgacca ggcgggtgcc gggctcgttg aacgcctgca ggtactcgtc gtggaagcag
     2821 gggcgcttgc acagctggcg gtaccagggc ttgaggttcg cggccgtcgc cgggtcttcg
     2881 acgatctcgt cgacccgggc gcggatctcg gtcatcttct cgtcgtcgct gtcctcgaag
     2941 gcgcgggcga cgagctcggg tgtgaactcg gcgccctcct tcgagagctc ggccaccacg
     3001 cggtcgcgga tgcgctggga gatgtcggtc cacccgtcct ccaccaggtc ctcgtcggtg
     3061 aagccgccgg tctggaggat ggtgaagttc atctgccagc ggcgctgcca cccgggctcc
     3121 aggtcaccga accactgggg gtcgatggcg tggttgctgc ggacgtcgat ggacgacggc
     3181 gtgcgctgga acacgaggag ctcccccgcg ccacgagcga ggtgggggat gcactgcacg
     3241 gccgtggcgc cggtgccgat gatgccgacg cgcttgtcgg ccagccggtc gagcggcgcg
     3301 ccggtggggt cgccgccggt gtacccgtag tcccagcggc tggtgtggaa ggtgtggccg
     3361 gcgaacgtct cgatgccggg gatgccgggg agcttgggct tctggagcgg gccggtgccc
     3421 atggccacga actgggcggt gaacgcgtcg cctcggtccg tgcggacggt ccagcgggag
     3481 gcgccgccgt cccactcgag cccggtgacc ttggtggaga agagggcgtg ctcgtacagg
     3541 ccgtactggg tggcgatggc gcgggcgtgg gcgaagttct cggccgcggg cgtgtacttc
     3601 gcgctcggga cgtgcccggt ctcctcgagg agcgggaggt a
//
LOCUS       DATMFS010000040         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_234000, whole genome shotgun sequence.
ACCESSION   DATMFS010000040 DATMFS010000000
VERSION     DATMFS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234000"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VNQ33_00760"
     CDS             <1..257
                     /locus_tag="VNQ33_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96654.1"
                     /translation="LLVGAALMFVVTGLACAFRKLWVSAAVFFLLAAGAAWWYQVTDE
                     LPREIIPYFPHVTTLLVLVFASQRLRMPAADGKVWRRGGR"
     gene            263..667
                     /locus_tag="VNQ33_00765"
     CDS             263..667
                     /locus_tag="VNQ33_00765"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWJ96655.1"
                     /translation="MPEPDWAALRVAAQQMLDAAYAPYSGVRVGAAALVDDGRIVTGC
                     NVENASYGLSLCAECGVAQALHASGGGRLVALAVVGPDGSYLAPCGRCRQILFEFGGR
                     ELLVETGDGIVTLDDLLPGAFGPHDLPPGRRP"
     gene            664..1440
                     /locus_tag="VNQ33_00770"
     CDS             664..1440
                     /locus_tag="VNQ33_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HWJ96656.1"
                     /translation="MTGPRQYHIGFGADDLPDGTTIALLSGDPNRSEHIAKERLDGGR
                     DLARNRGLDSFLGQLPGGRPVVCATSGMGAPSASIVVNELVQSGITTIIRIGTSGSIQ
                     PHVHIGSVVIGSAALTLQGAADDIAPPQYPAAADPFLTVALAHAAEDLGIDHHVGIMG
                     SVDTFFEGQERLDSSANPQLLRRHRGSIDEYQSLRVLNLEMEAGTLFKMGGVYGFTAG
                     CVVGIIAHRQHEERPDLAMKDVAVANAVEVAIATADTWVD"
     gene            1590..2153
                     /locus_tag="VNQ33_00775"
     CDS             1590..2153
                     /locus_tag="VNQ33_00775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ96657.1"
                     /translation="MTGRPRDEQLSFRPMVREDLPRLQAWITAPHVAQWWGDDSSDTI
                     QAVEAKYAERIDGTSSVSPWVMEIEGRPVGFIQWYRVEDEAEWYPGLAIPPGTVALDI
                     AIGDADYVGKGHGRRLVLEFVHHVVRTTLPDCPEVWIDPDPRNERAVRAYRAAGFHDT
                     GIDLPDPEHPGQVRRLMRMSWAGPTFH"
BASE COUNT          355 a          907 c          806 g          350 t
ORIGIN      
        1 ccctgctcgt cggcgcggcc ctgatgttcg tcgtcaccgg cctggcgtgt gccttccgga
       61 agctgtgggt atcggctgcg gtgttcttcc tgctcgccgc cggtgccgcc tggtggtacc
      121 aggtcaccga cgagctgccc cgggagatca tcccgtactt cccgcacgtc acgaccctct
      181 tggtcctcgt gttcgccagc cagcggctgc gcatgcctgc ggccgacggc aaggtctggc
      241 gccgaggcgg acggtgaccc cggtgcccga gcccgactgg gccgccctgc gggtggccgc
      301 ccagcagatg ctcgatgccg cctacgcgcc ctactcgggc gtgcgggtcg gcgcggcggc
      361 actcgtcgac gacgggcgga tcgtcaccgg ctgcaacgtg gagaacgcct cctacggcct
      421 gtcgctctgc gccgagtgcg gcgtcgccca ggcgctgcac gcatccggcg ggggccggct
      481 ggtggcgctc gccgtggtcg gtcccgacgg ctcctacctc gcgccgtgcg gccgctgccg
      541 ccagatcctc ttcgagttcg gcggccggga gctgctcgtc gagaccggcg acggcatcgt
      601 cacgctcgac gacctgctcc ccggtgcgtt cggcccccac gacctccccc caggacgacg
      661 gccatgaccg gcccccggca gtaccacatc ggcttcggcg cagacgacct gcccgacggc
      721 accaccatcg ccttgttgag cggcgacccg aaccgctccg agcacatcgc caaggagcgc
      781 ctcgacggcg gccgggacct cgcccgcaac cgcgggctcg actcgttcct cggccagctc
      841 cccggtggcc gaccggtcgt gtgcgccacc agcggcatgg gcgctccctc ggccagcatc
      901 gtcgtcaacg agctggtgca atcgggcatc accaccatca tccgcatcgg caccagcggc
      961 tcgatccagc cgcacgtgca catcggctcg gtcgtcatcg gctcggccgc gctcaccctc
     1021 cagggcgccg cggatgacat cgccccgccc cagtaccccg cggctgcaga cccgttcctc
     1081 accgtggccc tcgcccacgc cgccgaggac ctcggcatcg accaccacgt cgggatcatg
     1141 ggctcggtcg acacgttctt cgagggtcag gagcgcctgg actcgtcggc caacccgcag
     1201 ctgctccgcc gccaccgcgg cagcatcgat gagtaccagt cgctgcgggt cctcaacctg
     1261 gagatggagg ccggcaccct cttcaagatg ggcggggtct acggcttcac cgccggctgc
     1321 gtggtcggca tcatcgccca ccgccagcac gaggagcgcc ccgacctggc gatgaaggac
     1381 gtcgccgtcg ccaacgccgt cgaggtcgcc atcgccaccg ccgacacctg ggtcgactga
     1441 cccgctctgc gacccacccc gccctacccc ggaacttttc gtgaagttgg tggcagagcc
     1501 accacgatca cgaaaagttc aagacagcgg ggggagtggc ggcagaatgg gcgggtccgc
     1561 acccctggac aagaggatcg atcgatgcca tgaccggccg cccgcgtgac gagcagctca
     1621 gcttccgccc gatggtccgc gaggacctcc cccggctgca ggcgtggatc acggcacccc
     1681 acgtcgccca gtggtggggc gacgattcga gcgacaccat ccaggccgtc gaagccaagt
     1741 acgcggagcg catcgacggc accagctcgg tcagtccctg ggtgatggag atcgaaggca
     1801 ggccggtcgg gttcatccag tggtaccgcg tggaggacga ggccgagtgg tacccgggcc
     1861 tggccatacc gcccgggacc gtggccctcg acatcgccat cggcgacgcc gactacgtgg
     1921 gcaagggcca cgggcgccgg ctggtgctcg agttcgtcca ccacgtggtg cgcaccacgc
     1981 tccccgactg cccggaggtg tggatcgacc ccgacccccg caacgagcgg gccgtgcgcg
     2041 cctaccgggc ggcgggcttc cacgacaccg gcatcgacct ccccgacccc gagcaccccg
     2101 gccaggtgag gcgcctcatg cgcatgtcct gggccggccc caccttccac taggaggggt
     2161 cggcgaaact ccgtcggtcc agcggtggac gtgggactac ggtccggccg acgtcggacc
     2221 gtcgacgcga gggagcaaac agatgccagg cgctgaactg cgaggggtga cgctggactg
     2281 tgccgacccg caaacgctgg cggccttcta cggtgcactg accgggatgc gcgacctgtt
     2341 cagttcggag gactacgtcg ccctgacggc cgactccggc tgtgacttgg gattccagcg
     2401 agtggacgga taccgggc
//
LOCUS       DATMFS010000041         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_141032, whole genome shotgun sequence.
ACCESSION   DATMFS010000041 DATMFS010000000
VERSION     DATMFS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141032"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VNQ33_00780"
     CDS             <1..613
                     /locus_tag="VNQ33_00780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ96658.1"
                     /translation="DTLLAAAVRRVARGARVSTVSAPERDRAIALLDEATAILGQEET
                     PGPFWLTGLSSFEDFDLTASLATIHTYSPALGDANPFAPKVEMEVTADKVVQGTVTFA
                     EVHNGPPWNTCHGGIVALVYDDIVGLAAMVGAGGGMTANLSIDYRKPTPLFEPIRFKA
                     WMEWHEDRKHLARGEMHLGDTLLSEAKGLFIRPKGFPDGVSAG"
     gene            complement(617..1987)
                     /locus_tag="VNQ33_00785"
     CDS             complement(617..1987)
                     /locus_tag="VNQ33_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ96659.1"
                     /translation="MGHPQPAGAKRHGVVTERRSRQLLAVVVLAGFVLRVAWVLWASR
                     EPQDIFLDPTRYLGYAREIAAGHGMTEPLTSAPTAYYPPGYPWFLGIVTWLTQPFTDE
                     PWLVAGLVQALLGAASIGLVASVAKRLAGTTAALLGAVLYALYPNLIFHTGALLGETL
                     YNFLFLAFLALLLSRPWDAAFSTARILGCGVLLGLAVMVRPISLAVIPIVALCWLWSR
                     KDYRVTLRFTVVLVIGVVACIVPWTIRNEIRLHALVPISTNTGDNLCIGHADGATGAF
                     QPVDACQTHFDFKDGIPAELGSDKEKQRIALRGMVDHLDREPWLLWRRTYYMWVRDGD
                     HDGLFAVQEYRHNRWMALPTESRLIWIADRYYWLVVAAGLVGLVQLTRRKEPEAFVLV
                     GSTIMTAAVPLMFFGDQRFKVPVIPLLIIAAACLADGTWGRGQTSTHQTAEGDDELGT
                     GAECLG"
     gene            complement(1942..>2624)
                     /locus_tag="VNQ33_00790"
     CDS             complement(1942..>2624)
                     /locus_tag="VNQ33_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWJ96660.1"
                     /translation="DRFLRAVRSSAPDADLLVCDDSSPDGTGEIAEKTAAELGRVEVL
                     HRPGKEGLGAAYREGFRWGLDQGYDVIIQMDCDFSHDPAMIPTLLDAVGDGVDCAIGS
                     RYVPGGSTPGWPLHRRMLSKYGNRYTAGVLRLGIKDATGGFRAYRASTLEAIDIDSTR
                     ANGYAFQSEVALRMVQAGLRLEEIPITFVDREYGTSKMSVKIMAESMARVTTWGIRSR
                     LGRSATAS"
BASE COUNT          417 a          893 c          953 g          361 t
ORIGIN      
        1 cgacacgctc ctcgccgcgg ccgtccgccg agtcgcccgc ggcgctcgtg tcagcacggt
       61 ctcggccccc gagcgggacc gggccatcgc cctgctcgac gaggccaccg ccatcttggg
      121 ccaggaggag acccccggcc cgttctggct gacgggcctg tcgtcgttcg aggacttcga
      181 cctgaccgcc tcgctcgcca cgatccacac ctactccccc gccctcggcg acgcgaaccc
      241 gttcgccccg aaggtggaga tggaggtcac cgccgacaag gtggtgcagg gcacggtgac
      301 cttcgccgag gtccacaacg gtccgccctg gaacacgtgc cacggtggca tcgtcgccct
      361 cgtctacgac gacatcgtgg gcttggccgc catggtcggt gccggtggcg ggatgaccgc
      421 caacctgtcg atcgactacc gcaagcccac cccgctgttc gagccgatcc gcttcaaggc
      481 gtggatggag tggcacgagg accgcaagca cctcgcccgc ggcgagatgc acctcggcga
      541 cacgctgctc agcgaggcca agggcctctt catccgcccc aagggcttcc ccgacggcgt
      601 cagcgccggc tgaacgtcag cccaggcact cggccccggt gccgagctcg tcatcacctt
      661 cggcggtctg gtgggtggag gtctggccgc ggccccacgt gccgtcggcg aggcacgccg
      721 ccgcgatgat cagcagcggg atgacgggga ccttgaagcg ctggtcgccg aagaacatca
      781 gggggaccgc ggccgtcatg atcgtggaac cgaccaagac gaacgcctcg ggctccttgc
      841 gccgggtgag ctgcaccagg cccaccaggc cggcggcgac gaccagccag tagtaccgat
      901 cggcgatcca gatgaggcgg gactcggtgg ggagcgccat ccaacggttg tgccggtact
      961 cctgcacggc gaagagcccg tcgtggtcac cgtcgcggac ccacatgtag taggtccggc
     1021 gccagagcag ccacggctcc cggtcgaggt ggtcgaccat gccgcgcagg gcgatgcgct
     1081 gcttctcctt gtcgctgccg agctcggcgg ggatgccgtc tttgaagtcg aagtgggtct
     1141 ggcaggcgtc gaccggttgg aaggcgcccg tggcgccgtc ggcgtgaccg atgcagaggt
     1201 tgtcgcccgt gttggtggag atggggacga gggcgtgcaa ccggatctcg tttcggatcg
     1261 tccacggcac gatgcaggcg acgacgccga tcaccagcac gacggtgaag cggagcgtca
     1321 cccggtagtc cttgcgggac cacagccagc acagggccac gatcgggatg acggccaggg
     1381 agatgggccg gaccatcacg gcgaggccca gcaggacgcc gcaccccagg atgcgagcgg
     1441 tgctgaacgc ggcatcccac gggcgcgaga gcagcagggc gaggaaggcg aggaacaaga
     1501 agttgtagag cgtctcgccg agcagggcgc cggtgtggaa gatcaggttc gggtacaggg
     1561 cgtagaggac cgcgcccaag agtgcggcgg tggtgccggc gagccgcttg gcgaccgagg
     1621 ccacgaggcc gatcgacgcc gcgcccagca gagcctggac gaggccggcc acgagccacg
     1681 gctcgtcggt gaacggctgg gtcagccagg tgacgatgcc gaggaaccac gggtagccgg
     1741 gcgggtagta ggccgtcggc gcgctggtga gcggctcggt catgccgtgg ccggcggcga
     1801 tctcccgggc gtacccgagg taccgcgtgg ggtcgaggaa gatgtcctgc ggctcgcggc
     1861 tggcccagag gacccaggcg acccgcaaca cgaacccggc gaggacgacg accgccagga
     1921 gctggcggga gcggcgctcg gtcacgacgc cgtggcgctt cgccccagcc ggctgcggat
     1981 gccccacgtg gtcacccggg ccatggactc ggccatgatc ttcacggaca tcttcgaggt
     2041 gccgtactcc cggtccacga acgtgatcgg gatctcctcg aggcggagcc cggcctgcac
     2101 catgcgcagc gccacctcgc tctggaaggc gtacccgttg gcgcgggtgg agtcgatgtc
     2161 gatggcctcg agcgtcgacg cgcggtaggc ccggaacccg ccggtggcgt ctttgatgcc
     2221 gagccggagg acaccggccg tgtagcggtt gccgtacttg gagagcatgc ggcggtgcag
     2281 cggccagccg ggggtggagc cgccgggcac gtaccggctg ccgatggcgc agtcgacgcc
     2341 gtcacccacc gcgtcgagca gcgtggggat catcgccggg tcgtgcgaga agtcgcagtc
     2401 catctggatg atcacgtcgt agccctggtc gaggccccag cggaagccct cgcggtaggc
     2461 ggcccccagc ccctcctttc ccggccggtg cagcacctcg acgcggccca gctcggccgc
     2521 cgtcttctcg gcgatctcac cggtgccgtc ggggctgctg tcgtcgcaca ccagcagatc
     2581 agcgtccgga gcgctcgatc ggacggcccg caagaagcgg tcga
//
LOCUS       DATMFS010000042         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_24959, whole genome shotgun sequence.
ACCESSION   DATMFS010000042 DATMFS010000000
VERSION     DATMFS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24959"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VNQ33_00795"
     CDS             <1..861
                     /locus_tag="VNQ33_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96661.1"
                     /translation="PVDSPEVPPAARIEPPASPVARTKEWVSDVAVLTYRNLVHVRRE
                     PAQLSDATIQPVLFTLLFVYIFGSAMAIAGGGSYKMFAIGGLVTMNLTTASMGTAVGM
                     SSDVATGMMNRFRTLPMSRSAILAGRTISDLFAAALCGTIVLLTGLVIGWRPDHGFVG
                     VVEGMAVALLFAYALSWFTACLGLLVGDPESAQGIGLVILFPLAFLSTCFVPAAGLPT
                     FPRIIAEWNPVSAVAGSCRELFGNPNPASLSGVWPAQHPVLMAVGSSLVILAICVPLA
                     SRLLRKATTD"
     gene            complement(977..1615)
                     /locus_tag="VNQ33_00800"
     CDS             complement(977..1615)
                     /locus_tag="VNQ33_00800"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWJ96662.1"
                     /translation="MAQLRLGDDAPDFTAETTEGTVNFHEWKGDSWAILFSHPKDFTP
                     VCTTELGRVAALKGEWDKRNVKVIGISVDTSESHEGWAQDIKDVTGSELNFPLIADNE
                     KKVADLYGLIHPNASDTTTVRSVFVIGPDNKLKLELTYPASTGRNFDEILRVVDSLQL
                     TAGYKVATPVDWKQGEDVIIVPAVSDEDAKDLFPAGWEAKKPYLRVTPQPGK"
     gene            complement(1763..>2827)
                     /locus_tag="VNQ33_00805"
     CDS             complement(1763..>2827)
                     /locus_tag="VNQ33_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWJ96663.1"
                     /translation="DEEDVADAQETAAHGHGTKAVIAALAANLAIAVAKFAGFAVTAS
                     SSMLAEGVHSVADSGNQVLLLVGGKRAKKAATEEHPFGYGRERYFWSFVVALVLFSLG
                     GLFAVYEGLHKIEHPEPIDSPQWAFGILGFAILAEGLSFRTAAKEAGKLKGDASYWQY
                     IRRAKAPELPVVLLEDFGALIGLVLAMGGISASVVTDDGRWDGYGSLAIGVLLVIIAM
                     VLVVEMKSLLIGEGASRKDVEAIRAAIEIEPDVIRVIHLRTEHLGPEELLVGTKVEFV
                     HELTVPEVSAAIDRVERSIRAGVPSARLIFVEPDVHDDHRVGRFVEEHTGYIDADDPH
                     YADITGTVPAIDVDDDIWTE"
BASE COUNT          376 a          984 c          981 g          486 t
ORIGIN      
        1 cccgtcgatt cccccgaggt gccgccggcg gcccgcatcg aaccgccggc ctcgcccgtc
       61 gcccggacca aggagtgggt gtccgacgtc gccgtcctca cctaccgcaa cctcgtgcac
      121 gtccgccggg agccggccca gctgtccgac gccaccatcc agccggtcct gttcaccctg
      181 ctgttcgtct acatcttcgg ctcggccatg gccatcgccg gcggcggctc ctacaagatg
      241 ttcgccatcg gcggcctggt caccatgaac ctcacgacgg catcgatggg caccgccgtg
      301 ggcatgagct ccgacgtggc caccgggatg atgaaccggt tccggacgct gccgatgtcg
      361 cgctcggcca tcctcgccgg gcgcaccatc tcggacctgt tcgccgccgc cctctgcggc
      421 acgatcgtgc tgctcaccgg cctcgtcatc ggctggcgcc cggaccacgg gttcgtcggc
      481 gtggtggagg gcatggccgt cgcgctcctc ttcgcctacg ccctcagctg gttcaccgcc
      541 tgcctcggcc tgctggtggg cgaccccgag tccgcccagg gcatcggcct ggtgatcctc
      601 ttcccgctgg cgttcctgtc gacctgcttc gtgcccgccg ccggcctgcc gaccttcccc
      661 cggatcatcg ccgagtggaa cccggtgtcc gcggtggccg gctcctgccg cgagctcttc
      721 ggcaacccca acccggcgtc gctgagcggt gtctggcccg cccagcaccc ggtcctcatg
      781 gccgtcggaa gcagcctggt catcctcgcc atctgcgtcc ccctcgccag ccgcctgctt
      841 cgcaaggcca ccaccgattg accgaacgcc atggccgcgg acgcgacgaa gggcggtccc
      901 gggtggaggg gaccgccctt cgtggcgtct tcgcccgatc cggccggcga gggggagcgc
      961 tggccggcgg gcgtgactac ttgccgggct ggggcgtgac ccgcaggtac ggcttcttgg
     1021 cctcccagcc ggcggggaag aggtccttgg cgtcctcgtc agacacggcc gggacgatga
     1081 tgacgtcctc gccctgcttc cagtccaccg gggtcgccac cttgtagccg gcggtgagct
     1141 ggagcgagtc caccacgcgg aggatctcgt cgaagttgcg gcccgtggag gcggggtagg
     1201 tgagctccag cttgagcttg ttgtccgggc cgatcacgaa caccgagcgg acggtggtgg
     1261 tgtcggaggc gttcgggtgg atgaggccgt acaggtcggc gaccttcttc tcgttgtcgg
     1321 cgatgagcgg gaagttcagc tcggagccgg tgacgtcctt gatgtcttgg gcccatccct
     1381 cgtggctctc ggaggtgtcc acggagatgc cgatgacctt cacgttgcgc ttgtcccact
     1441 cgcctttgag ggcggccacg cggccgagct cggtggtgca caccggcgtg aagtccttgg
     1501 ggtgcgagaa caggatcgcc cagctgtcgc ccttccactc gtggaagttg acggtgcctt
     1561 cggtggtctc tgcggtgaag tcgggggcgt cgtcgcccag tcgcagttgt gccatgggag
     1621 tttccttctg ggtggtcttc gttggtgtcg gagcggtcaa cctagtcggt cgccccgcaa
     1681 ttcccgacca accccatcag gtattcggcg tgaggtcaag ggcgagcagc gggtcacccg
     1741 tcacggtgcc cgtgcacggc ggctactcgg tccagatgtc gtcgtcgacg tcgatcgccg
     1801 ggacggtgcc ggtgatgtcg gcgtagtgcg gatcgtcggc gtcgatgtag ccggtgtgct
     1861 cctcgacgaa gcggccgacc cggtggtcgt cgtgcacgtc gggttccacg aagatgagcc
     1921 gggccgacgg cacgccggca cggatgctcc gctcgacgcg gtcgatggcc gcggacacct
     1981 cgggcacggt gagctcgtgg acgaactcga ccttggtgcc gaccaggagc tcctcggggc
     2041 cgaggtgctc cgtgcgcagg tggatgaccc ggatgacgtc gggctcgatc tcgatggcgg
     2101 cccggatggc ctcgacgtcc ttgcgggacg cgccctcgcc gatgagcagg ctcttcatct
     2161 cgaccaccag caccatggcg atgatcacga gcagcacgcc gatggccagc gagccgtagc
     2221 cgtcccagcg gccgtcgtcg gtcacgaccg aggcgctgat gccgcccatg gcgagcacga
     2281 ggccgatgag cgcgccgaag tcctcgagca gcaccaccgg cagctcgggc gccttggccc
     2341 ggcggatgta ctgccagtag ctggcgtcgc ccttgagctt gccggcttct ttggccgcgg
     2401 tccgaaacga caggccctcg gccaagatgg cgaagccgag gatgccgaac gcccactgcg
     2461 ggctgtcgat gggctcgggg tgctcgatct tgtggaggcc ctcgtagacg gcgaacaggc
     2521 cgccgaggga gaacagcacc agcgccacga cgaagctcca gaagtaccgc tcgcggccgt
     2581 agccgaacgg gtgctcctcg gtggcggcct tcttggcccg cttgccgccg accagcagca
     2641 ggacctggtt gccggagtcg gcgaccgagt gcacgccctc cgccagcatc gacgacgagg
     2701 ccgtgacggc gaacccggcg aacttggcga cggcgatcgc caggttggcg gccagcgcag
     2761 cgatgacggc cttggtgccg tggccgtgcg cggcggtctc ctgggcgtcg gcgacgtcct
     2821 cctcgtc
//
LOCUS       DATMFS010000043         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_24961, whole genome shotgun sequence.
ACCESSION   DATMFS010000043 DATMFS010000000
VERSION     DATMFS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24961"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /gene="rpsA"
                     /locus_tag="VNQ33_00810"
     CDS             <1..1149
                     /gene="rpsA"
                     /locus_tag="VNQ33_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWJ96664.1"
                     /translation="DIGYKSEGVIPARELSIRNDVDPSEIVAVNDVIEALVLQKEDKE
                     GRLVLSKKRAQYERAWGDIEKKKEEDGVVSGPVIEVVKGGLIIDIGLRGFLPASLVEL
                     RRVRDLAPYVGRTLEAKIIELDKNRNNVVLSRRAWLEETQKEQREEFLENLKPGEIRQ
                     GVVSSVVNFGAFVDLGGMDGLVHVSELSWKHVDHPGSVVAVGDEITVQVLEVDLDRER
                     ISLSLKATQQDPWQEFASGHRVGELVYGRVTKLVPFGSFVQVGDGIEGLVHISEMSAH
                     HVDLPEQVVTPGEELWVKIIDLDLQRRRISLSIKQAAEGGTVSAEYQEHFGEHAYDDE
                     GNFIGGETSEAQEAWAEYYAEHPEALAGEAAAEGEAVEAEVAEAAPEA"
     gene            1341..2204
                     /gene="cpaB"
                     /locus_tag="VNQ33_00815"
     CDS             1341..2204
                     /gene="cpaB"
                     /locus_tag="VNQ33_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWJ96665.1"
                     /translation="MSQRRTLILIAAIAIGALASFLVWNYVGGIQDQAYNDAERVKVY
                     LVKQPIPRGTSGQVAAASIVEESIPKKFKPANAIVSLEDISGRVAKSDLVANQVVVDD
                     MFVEATDPEAAQSFADNLIKIQNQDQTTYTMSVDNVRGVNGLIVPGDYVNVMVTNFSE
                     IAPTDDAGDEPQTVSSEQARFLYQKVQVLAVDYLTSSDINAAAATAPADGAAATAPTA
                     DVQRGMITLIVPPEAAQILLSIPPENLYLTLVSKDYNPKATGEVDITTLPSDNPKIVT
                     PYGPDGAKSSN"
     gene            2344..>2599
                     /locus_tag="VNQ33_00820"
     CDS             2344..>2599
                     /locus_tag="VNQ33_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96666.1"
                     /translation="MSTFPTSDTSTPIVVVEADPSVRSRLSMQLGERAIALETLDGVE
                     ERFTSPFLLVLGPSCALVPGVTGAEGMLARRADIGAILIAE"
BASE COUNT          463 a          914 c          850 g          372 t
ORIGIN      
        1 gacatcggct acaagtccga gggcgtcatc cccgctcgcg agctgtcgat ccgcaacgac
       61 gtcgatccct ccgagatcgt cgccgtcaac gacgtgatcg aagccctcgt cctccagaag
      121 gaggacaagg agggccgcct cgtcctgtcc aagaagcggg cgcagtacga gcgggcgtgg
      181 ggcgacatcg agaagaagaa ggaagaggac ggcgtcgtgt cgggcccggt gatcgaggtc
      241 gtcaagggcg gcctcatcat cgacatcggc ctccggggct tcctccccgc ctccctggtg
      301 gagcttcgcc gggtgcgcga cctcgccccc tacgtcggcc ggaccctcga ggccaagatc
      361 atcgagctgg acaagaaccg caacaacgtg gtcctgtccc gccgggcgtg gctcgaggag
      421 acgcagaagg agcagcgcga ggagttcctc gagaacctca agccgggtga gatccgccag
      481 ggcgtcgtgt cgagcgtcgt caacttcggt gcgttcgtgg acctcggcgg catggacggc
      541 ctcgtgcacg tgtccgagct ctcctggaag cacgtcgacc accccggctc ggtcgtggcc
      601 gtcggcgacg agatcaccgt ccaggtgctc gaggtcgacc tcgaccggga gcgcatcagc
      661 ctctcgctca aggccaccca gcaggatccg tggcaggagt tcgcctccgg tcaccgcgtg
      721 ggcgagctcg tctacggccg ggtcaccaag ctggtgccgt tcggctcgtt cgtccaggtg
      781 ggcgacggca tcgagggcct cgtgcacatc tcggagatgt cggcgcacca cgtcgacctt
      841 cccgagcagg tcgtcacccc gggcgaggag ctgtgggtga agatcatcga cctcgacctc
      901 cagcgccgcc ggatcagcct gtcgatcaag caggcggccg agggcggcac cgtgtccgcc
      961 gagtaccagg agcacttcgg cgagcacgcc tacgacgacg agggcaactt catcggcggc
     1021 gagacctccg aggcccagga ggcctgggcg gagtactacg ccgagcaccc tgaggccctc
     1081 gccggcgaag cggcggcgga aggcgaggcc gtcgaagccg aggtggccga ggcggctccg
     1141 gaggcctgac gccgccggcg tccgaacgaa cctgacggtg ccccgcctcc gggcggggca
     1201 ccgccgcgtt cgggggacgg catgggccgg ttggaccaga aatcaccacg aaacccttgc
     1261 gccggacgat ggtccggccg acagagaacc tgtcgcagca ctccgacctc ggagtgccct
     1321 caacctcgaa ggggttcacg gtgagccaac gccggacact cattctcatc gccgccatcg
     1381 ccatcggtgc actcgcctcg ttcctcgtct ggaactacgt cggcggcatc caggaccagg
     1441 cctacaacga cgcggagcgc gtcaaggtgt acctcgtgaa gcagccgatc ccccggggga
     1501 cgagcggcca ggtggctgcg gcctcgatcg tcgaggagag catcccgaag aagttcaagc
     1561 cggcgaacgc catcgtcagc ctcgaggaca tctcgggccg ggtggccaag agcgacctcg
     1621 tcgccaacca ggtggtcgtc gacgacatgt tcgtcgaggc gaccgatccg gaagcggcgc
     1681 agtcgttcgc cgacaacctc atcaagatcc agaaccagga ccagacgacc tacacgatga
     1741 gcgtggacaa cgtccggggc gtcaacgggc tcatcgtgcc cggtgactac gtcaacgtga
     1801 tggtcacgaa cttctccgag atcgccccaa ccgacgacgc cggcgacgag ccgcagaccg
     1861 tgtcgagcga gcaggcccgg ttcctgtacc agaaggtcca ggtgctggcg gtcgactacc
     1921 tcacgtcctc ggacatcaac gcggcggcgg cgaccgcccc cgctgacggc gccgcggcca
     1981 ccgcgccgac cgccgacgtc cagcggggca tgatcaccct gatcgtcccg cccgaggccg
     2041 ctcagatcct gctgtcgatc ccgccggaga acctctacct caccttggtc tcgaaggact
     2101 acaaccccaa ggcgaccggc gaggtcgaca tcaccaccct gccgtccgac aacccgaaga
     2161 tcgtgacccc ctacggtccg gacggggcca agagcagcaa ctgatcaagc ggatgcgctc
     2221 tgcttcgatc cctgccgtgc agccatccaa tccccccacc aggttcggac cacgatcatg
     2281 agcgagtacc tttgggcgga agatccgcct ccgccgagcg gaccaacgca agcgccgagg
     2341 gccatgtcga cgttccccac atctgacacc tccaccccga tcgtggtcgt cgaggccgat
     2401 ccctcggtgc gcagccgcct gtcgatgcag ctgggcgagc gggccatcgc ccttgagacg
     2461 ctcgacgggg tcgaggagcg gttcacctcg cccttcctgc tcgtgctcgg gccgtcgtgc
     2521 gccctggtcc ccggggtcac cggtgccgag ggcatgctgg ctcgccgggc cgacatcggc
     2581 gccatcctca tcgccgaag
//
LOCUS       DATMFS010000044         2557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_280675, whole genome shotgun sequence.
ACCESSION   DATMFS010000044 DATMFS010000000
VERSION     DATMFS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280675"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2524)
                     /locus_tag="VNQ33_00825"
     CDS             complement(<1..2524)
                     /locus_tag="VNQ33_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HWJ96667.1"
                     /translation="MPPFVEVDPDSVPADRLRVIAVVDDTLESWPSTVYVPVKVGKPS
                     VAVHVGAQAIGLSWSSADARLDATYLVEEEDGADWVAVPGTSLSADGTTVGIPYEPVD
                     ADSTRTYRVTRVRHLADGAHASIPSDPVEAHLRVLPQAPTVIQPVCGGEAPVNCVESE
                     AGNTYTIQWSPIPGDDTWPVAADRYEVEIALADGWLRLPDTAFPATAGQVDGVFQARA
                     DHRIDIYLRLVAVAADGRESLPSQAIDIYQPGEPPVGPDDPDPDAPPLSIRFVQTGEP
                     EGTDVVTDDDRIPNDMLGYTDGAFGFTPGSNGMVDVHASVGDISITEGTRDNLLDTVI
                     ARTDINAGDCSEACEGNQGGPTYVDPATGEQITFYHSEDYRLGYTDSGGHFNAVGMAY
                     APAGSTAFERRGFVYETEQRRDSGHTNAESGTGQFVVKDGYIYLYFTDSPDDETVHHE
                     GMGVARAPVAEVLAAARDDDHLQVPGEDGNPPAFDGLFKKYYDADPADDQPGAFSENA
                     IGGRSTEVSKSGGGGSVVYDACMDEVWIVTADNIEGGPPPAPAGGWPVATSITVSPDG
                     IHFDAPETIAITHGDRVYSTPAWRDARDPMVIRGDQFDVFARRHPRTDAARDDHTINR
                     LSFRDTLASQGCLGQPDSNGDPAEPVRDTRPKLSVTPATTAYEQVGGGLARFDLSLDV
                     PLDHDLTVSYWTSDRTATAVRGEYEPAGTQASPETLTIPAGDVQATVTVPVLDADAPV
                     SYHPQYGNPLPEQFALHIRTADGTVQMGRTLGVATIIDRATPDGTVPWLRLTPTVQVH
                     DGDSGTRTATVTLVLSQPVAQDVTVRLSTSDLTAHAGVHYEAV"
BASE COUNT          324 a          797 c         1003 g          433 t
ORIGIN      
        1 tcaccgcctc gtagtggacc ccggcgtggg cggtcaggtc gctcgtgctg agccggaccg
       61 tcacgtcctg agcgaccggc tgggacagga ccagggtgac cgtggcggtg cgggtgccgc
      121 tgtcgccgtc atggacctgg accgtcgggg tgaggcgcag ccacggcacg gtgccatccg
      181 gcgtggcccg gtcgatgatg gtggccacgc cgagggtccg tcccatctgg acggtgccgt
      241 cggcggtgcg gatgtggagt gcgaactgct cgggcagtgg gttgccgtac tgggggtggt
      301 acgagaccgg tgcgtcggcg tcgaggacgg gaacggtcac ggtggcctgg acgtcaccag
      361 cggggatggt gagcgtctcc gggctggctt gggttccggc cggttcgtac tcgcctcgga
      421 cggcggtggc ggtgcggtcg ctcgtccagt agctgaccgt gaggtcgtgg tcgaggggca
      481 cgtcgagcga gaggtcgaag cgggcgaggc ctccgccgac ctgctcgtag gccgtggtcg
      541 cgggggtgac cgagagcttg ggacgggtgt ctcggaccgg ttcggccgga tcgccgttgc
      601 tgtcgggctg gccgaggcag ccttggctgg ccagggtgtc gcggaagctc aaccggttga
      661 tggtgtggtc gtcgcgagca gcgtcggttc gggggtggcg tcgggcgaag acgtcgaact
      721 ggtcgccgcg gatgaccatg gggtcacggg catcgcgcca ggccggggtg ctgtagacgc
      781 ggtcgccgtg cgtgatggcg atggtctccg gcgcatcgaa gtggatcccg tccggtgaga
      841 cggtgatgga ggtggcgacc ggccagcctc cagcgggtgc cgggggcgga ccgccctcga
      901 tgttgtcggc ggtgacgatc cagacctcgt ccatgcaggc gtcgtagacg acgcttccgc
      961 cgcctccgct cttggagacc tcggtcgacc tcccgccgat ggcgttctcg ctgaaggccc
     1021 cgggctggtc gtcggcgggg tcggcgtcgt agtacttctt gaacaagccg tcgaaggccg
     1081 gagggttgcc gtcctcgccg ggcacctgga ggtgatcgtc gtctcgggcg gcggcgagca
     1141 cctcggcgac cggcgcccgg gcgacgccca tcccctcgtg gtggacggtc tcgtcatcag
     1201 gggaatcggt gaagtacagg tagatgtagc cgtccttgac gacgaactgg ccggtgcccg
     1261 actcggcgtt ggtgtgcccg ctgtcgcgcc gctgctcggt ctcgtacacg aagccgcggc
     1321 gctcgaacgc ggtggagccg gccggggcgt acgccatgcc gacggcgttg aagtgcccgc
     1381 cgctgtcggt gtatccgagg cggtagtcct cggagtggta gaaggtgatc tgctcgcccg
     1441 tggccggatc gacgtaggtc ggcccgccct ggttgccctc gcaggcctcg ctgcagtcac
     1501 cggcgttgat gtcggtgcgg gcgatgacgg tgtcgagcag gttgtcccgc gtcccttcgg
     1561 tgatcgagat gtccccgacc gaggcgtgga cgtcgaccat gccgttgctg ccgggcgtga
     1621 agccgaaggc gccgtcggtg tacccgagca tgtcgttggg gatccggtcg tcgtcggtga
     1681 cgacatcggt tccttcgggc tctcccgtct ggacgaaccg gatcgagagc gggggcgcgt
     1741 ccgggtccgg atcgtcgggc cccaccggcg gctcgcctgg ttggtagatg tcgatggcct
     1801 ggctgggaag gctctcgcgc ccgtcagcgg cgacggcgac gaggcggagg tagatgtcga
     1861 tgcggtggtc ggctcgggcc tggaagacgc catcgacctg tccggcggtc gccgggaagg
     1921 ccgtgtccgg cagtcgcagc cacccgtcgg cgagggcgat ctcgacctcg tagcggtcgg
     1981 cagcgacggg ccaggtgtcg tcgcctggga tcggagacca ctggatggtg tacgtgttcc
     2041 ccgcttcgga ctcgacgcag ttcaccggtg cctcgccgcc gcacaccggc tggatcaccg
     2101 tcggtgcctg tggcagcacc cgcaggtggg cctcgacggg atccgacggg atgctcgcgt
     2161 gcgctccgtc ggccagatgc cggacgcggg tgacgcggta ggtccgcgtg ctgtcggcgt
     2221 cgacgggctc gtacgggatg ccgaccgtgg tcccgtcggc gctcagcgag gtgccgggca
     2281 cggcgaccca atcggcgccg tcctcctcct cgacgaggta ggtggcatcg agacgggcat
     2341 cggcgctcga ccagctgagc ccgatcgcct gcgcaccgac gtggaccgca acgctgggct
     2401 tgccgacctt gaccggcacg tacacggtgg acggccagct ctcgagggtg tcgtccacga
     2461 cggcgatgac ccggagccgg tccgcgggca ccgaatccgg atccacctcg acgaacggag
     2521 gcacggcatc agccaggatc gtgctcgtgg ccaggtc
//
LOCUS       DATMFS010000045         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_280676, whole genome shotgun sequence.
ACCESSION   DATMFS010000045 DATMFS010000000
VERSION     DATMFS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280676"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VNQ33_00830"
     CDS             complement(<1..463)
                     /locus_tag="VNQ33_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96668.1"
                     /translation="MPARPEVMLGSQVWDPRAAASAASAFATGLTVIVLIVTAERLFS
                     GKQAKGALSVAIAPVALAMQLTTAYFFVLIAGADPGDDQTVVSLSALLFTFAGSMLAM
                     SAVVMFFIVAHGANERLDPRNQLARQSVKATYFIVVAITSLFLVFGYDDIRS"
     gene            complement(463..1227)
                     /locus_tag="VNQ33_00835"
     CDS             complement(463..1227)
                     /locus_tag="VNQ33_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ96669.1"
                     /translation="MADPESSPRSEQGRTVVLVHGAWHGAWCWDRWLEPLTTAGYRVR
                     TLDLPGHGDPGARRLWTSLRSSVDALEAVVDGLESDVTLVGHSMGGLVVQRLLERRSV
                     AGAVLLASVPRRGVTGTTVRQLRRHPLRTLRAIVTLDLWPLVNSPERTRELFFTDSSS
                     EETVARTAARLQNESFLAYLSMMVRWPRSARVTTPVRVVAAGRDAVFSLAEQKGLAAA
                     YGTDLVVVPEAGHDLMLDDTAAETAGVVARLIATFP"
     gene            complement(1604..>2848)
                     /locus_tag="VNQ33_00840"
     CDS             complement(1604..>2848)
                     /locus_tag="VNQ33_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96670.1"
                     /translation="SQDRYVTPYNMVDQGPDALFVYGGGYGDSPDASGSFVSRVAPDT
                     FNEVWRRVLINTKTTGEWNYPGVLNTMADGGLVVIFGHQIAKLDAQTGSVLARATLPT
                     GSSKPENTAYNGFDALPDGTIIAKTVNRQPGCTEQGFSAFLQCPDPASAPASVLVAID
                     PATLEVIDQVELPEMMGGRVTTTVSDDGDARIYLPGATKLYRYTFADGRFAVDTGWGP
                     VSYLKDGQTAASAMAVIGDYVVGMTNGGAPTSTPMSVFAVSQTDSSKVANLQPFADAG
                     ADNSFIPSMVSVDPDNQRVYVMDAGAGKLGAIDLENGKLTTAWTQDQRTLSFTTLVGP
                     SDKRVLIGTDIPVRTFKGLQDYTVEQVVWRDADSGKELARTSDLPKMTTGILVTPGYG
                     GRQHYLAADGQIFDLQVLPTAD"
BASE COUNT          456 a          895 c         1025 g          472 t
ORIGIN      
        1 cggagcggat gtcgtcgtag ccgaacacga ggaaaagcga ggtgatggcg acgacgatga
       61 agtaggtggc cttgacggac tgacgggcca gctggttgcg cgggtcgagc cgttcgttcg
      121 cgccatgggc gacgatgaag aacatcacca ccgcggacat cgcgagcatc gacccggcga
      181 aggtgaacag gagcgcagag agcgagacga ccgtctggtc gtcgccaggg tcggccccgg
      241 cgatgagcac gaagaagtag gcggtggtga gttgcatggc gagcgcgacc ggggcgatcg
      301 caacgctgag cgcacccttc gcctgcttcc cgctgaagag acgttccgcg gtgacgatca
      361 gcacgatcac cgtgaggcca gtcgcgaagg cgctcgccgc tgatgcggct gctcgcggat
      421 cccagacctg gctccccagc atcacctcgg gtcgggctgg catcacggga aggttgcgat
      481 gagcctggcc acgaccccgg cggtctccgc agcggtgtca tcgagcatca gatcgtggcc
      541 ggcctcgggg accacgacca gatcggtgcc gtaggcagcc gccaatccct tttgctcggc
      601 gaggctgaac accgcgtcac gaccggccgc caccaccctg accggtgtcg tgacccgtgc
      661 cgaccgtggc cagcgcacca tcatcgagag gtaggccagg aaggactcgt tctgaaggcg
      721 agcagcggtg cgggccacgg tctcctccga cgagctgtcg gtgaagaaga gctcgcgtgt
      781 gcgctccggt gagttcacca gcggccacag gtcgagcgtg acgatggccc gcagcgtgcg
      841 cagcggatgt cggcgcaact ggcgcaccgt cgtgccggtg acgccgcgtc gcgggaccga
      901 tgccagcagc accgctccgg cgacgctccg tcgctccagg agtcgttgga ccacgaggcc
      961 acccattgag tgtccgacga gcgtcacgtc cgactccagg ccgtcgacga ccgcctcgag
     1021 cgcatcgacg gaggaacgga gtgatgtcca cagccgtctc gctccggggt caccgtggcc
     1081 gggaaggtcc agggtccgca cccggtagcc agcggtcgtc aacggctcca gccagcggtc
     1141 ccagcaccag gcaccgtgcc acgcaccgtg gaccagcacg acggttcggc cctgttcgct
     1201 tcgtgggctg ctctcggggt ccgccatggt cacgggacca tcatccacca tgccgccgcg
     1261 gcccactcgc acgcgattcg ctgttggacg gtctccggta gctgactgcc ggagcaaggt
     1321 gttggactgc cggtcgtgga acggctgcgc gacgtcttgg gcaggtgatc gccgctctcg
     1381 ggtcctggac cgtgcccatc agactcggag cgacgcagag caccccctga tcacgtctcg
     1441 ttcgaaccac gtggtgcgag atgtgctggc gaggctggtc gatcaggaac agtcgagtct
     1501 tgatcggaga tccggcctcc catgggtagg agaggtgctt gcggacggcc tgggggaggt
     1561 gccggtcggc tctggctcac cgccggtgca tcgcggagcg tcgtcagtcg gctgtcggca
     1621 ggacctgaag gtcgaagatc tggccgtcgg cagcgaggta gtgctggcgc ccgccatagc
     1681 cgggggtcac gagaatgccg gtggtcatct tcgggaggtc actggtccga gccagttcct
     1741 tgccggagtc ggcgtcacgc cacaccacct gttcgacggt gtagtcctgc aggcccttga
     1801 acgtgcgcac ggggatgtcg gtgccgatga ggacccgttt gtcggacgga cccacgaggg
     1861 tggtgaagct gagcgtgcgc tggtcttggg tccacgccgt ggtgagcttg ccgttctcga
     1921 ggtcgatcgc gccgagcttc ccggcccccg cgtccatgac gtagacgcgc tggttgtcag
     1981 ggtcgaccga caccatcgac gggatgaagc tgttgtcggc ccctgcatcg gcgaacggtt
     2041 ggaggttcgc gaccttcgac gagtccgtct ggctgaccgc gaagacgctc atgggagtgg
     2101 aggtgggagc gccgccgttg gtcatgccga cgacgtagtc gccgatgacc gccatcgcgg
     2161 aggcggcggt ctgcccgtcc ttcaggtagg agacgggtcc ccacccggtg tcgacggcga
     2221 acctgccgtc agcgaaggtg tagcggtaga gcttcgtggc gcctgggagg tagatccgcg
     2281 cgtcgccgtc gtcggagacg gtggtggtga cccggccgcc catcatctcc ggaagctcga
     2341 cctggtcgat gacctccagg gttgcagggt cgatggcgac gaggaccgac gcgggcgctg
     2401 atgcggggtc cggacactgg aggaacgcgg agaacccctg ctcggtgcat ccgggctggc
     2461 ggttgacggt cttggcgatg atggtgccgt cggggagggc gtcgaagccg ttgtacgccg
     2521 tgttctccgg cttcgacgaa ccggtgggca gcgtcgcccg tgcgaggacc gatcccgtct
     2581 gggcgtcgag cttcgcgatc tggtggccga agatcaccac gagcccaccg tcggccatcg
     2641 tgttgaggac gccggggtag ttccactctc ccgtcgtctt ggtgttgatc agaacccgtc
     2701 gccagacctc gttgaaggtg tcgggggcga ccctcgacac gaacgagccg gatgcgtcag
     2761 ggctgtcgcc gtagcctccg ccgtagacga acagcgcgtc gggcccctgg tcgaccatgt
     2821 tgtacggcgt gacgtagcgg tcctggga
//
LOCUS       DATMFS010000046         2632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_257135, whole genome shotgun sequence.
ACCESSION   DATMFS010000046 DATMFS010000000
VERSION     DATMFS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257135"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VNQ33_00845"
     CDS             <1..413
                     /locus_tag="VNQ33_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HWJ96671.1"
                     /translation="STLLLIALPPTDARTAHARIDVVGALRLTGYVARQLVTSNVLMI
                     REILRPHLATEPGVLAHRLQQPSEHVVTVMTSVISLSPGTMVVDVDRASSTVDVHFLF
                     LHDTDAARRSLERLEHVCRGAIRGTDAPSTRGAP"
     gene            410..661
                     /locus_tag="VNQ33_00850"
     CDS             410..661
                     /locus_tag="VNQ33_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HWJ96672.1"
                     /translation="MTQVTFVILGIAAVLFMYRLCAGPSLADRVVALNGVVLVGMAGI
                     ATHAAHTGVGAFLPTLVAIALVGPISNGMIARYIEGRQP"
     gene            658..978
                     /gene="mnhG"
                     /locus_tag="VNQ33_00855"
     CDS             658..978
                     /gene="mnhG"
                     /locus_tag="VNQ33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HWJ96673.1"
                     /translation="MIGEILALAGAVLVLLSAIGVVRFDDVLARLHALAKASTLGILL
                     VLLGAAVNLHDLNDITSVVLAAVLHLFASPPASNMISRAAYLTSDLSSGTDERTPDQQ
                     RAGS"
     gene            complement(1069..1533)
                     /locus_tag="VNQ33_00860"
     CDS             complement(1069..1533)
                     /locus_tag="VNQ33_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ96674.1"
                     /translation="MAAASNEGAGRTGPGEAELARLWSDVAEAVVIAEPAGTIVFWND
                     AAERLFGWPGAEARGQTLDLIIPARLRSRHWAGYERVMETGETSYGDRLLEVPAIHRD
                     GRTLSVAFTVTLIVGPGHGVDFIVAVLRDETERWNERRDLLARLRGQDTSEA"
     gene            complement(1542..2297)
                     /locus_tag="VNQ33_00865"
     CDS             complement(1542..2297)
                     /locus_tag="VNQ33_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HWJ96675.1"
                     /translation="MLVPTEHGGWGLTLEPVLLGLLVAPSWSGAGLGLAAVVLFLART
                     PLKVLLVDRHRRRWLPRTRMAGVSVGCYGLVLAGCAAVPLLQAPGSTWLPLVLIAPLV
                     GAELWYDMRSRGRRLVPELAGAIGIAGVAAMIVLADGQPAGIAAACWVLLAARAVTSI
                     VNVRDLVQGLHGRPRQPAAVWAGDAIALGLAAAAVALEPAAVVGAVAVAALIVVQRLL
                     RLGPTPRAVILGLTQTGLGLVVVGAVAAGMIQA"
     gene            complement(2390..2521)
                     /locus_tag="VNQ33_00870"
     CDS             complement(2390..2521)
                     /locus_tag="VNQ33_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96676.1"
                     /translation="MLQPIDPDRLRDARPAHPMRASIILVTAVAVLIVVLAVTSSLA"
BASE COUNT          377 a         1032 c          892 g          331 t
ORIGIN      
        1 tgtccacctt gttgctgatc gccctgccgc ccaccgacgc ccgcacggcc cacgcccgga
       61 tcgacgtcgt cggcgcgctg cgcctcaccg ggtacgtggc ccggcagctc gtcacctcca
      121 acgtgttgat gatccgcgag atcctgcgcc cgcacctggc cacggagccc ggcgtcctcg
      181 cccaccggct gcagcagccg tccgagcacg tggtcaccgt gatgaccagc gtgatctcgc
      241 tcagccccgg gacgatggtg gtggatgtgg accgggcctc cagcacggtg gacgtgcact
      301 tcctcttcct gcacgacacc gacgccgccc gtcgatcgct cgagcgcctc gagcacgtgt
      361 gccgcggcgc catccgaggc accgatgccc cgagcacccg tggagcgcca tgacccaagt
      421 caccttcgtc atcctcggca tcgctgccgt gctgttcatg taccggctct gcgccggccc
      481 ctcgctcgcg gaccgcgtgg tcgccctcaa cggcgtcgtg ctcgtcggca tggccgggat
      541 cgccacccat gccgcgcaca ccggcgtggg cgcgttcttg ccgaccctcg tcgcgatcgc
      601 cctggtgggg cccatcagca acggcatgat cgcccgctac atcgagggtc gccaaccgtg
      661 atcggcgaga tcctcgccct cgccggcgcc gtcctcgtgc tgctctccgc gatcggcgtc
      721 gtccgcttcg acgacgtgct cgcccgcctc catgcgctgg ccaaggcgtc caccctcggc
      781 atcttgctcg tgctgctggg cgcggcggtg aacctccacg acctcaacga catcacctcg
      841 gtcgtcctcg ccgctgtcct ccacctgttc gcctctcccc cggcttcgaa catgatcagc
      901 cgggccgcgt acctcacctc agacctgtcg agcggcaccg acgagcgcac gccggaccag
      961 cagcgagcag gctcgtgacg aggcgcgcgg ccggcgatca cccgcatggc accggtccga
     1021 gaccgacgtc atgccgggag accacgagga gcgcggcggg cgctgacctc acgcctccga
     1081 cgtgtcctgg cccctcagcc gggcgagcag gtcccggcgc tcgttccacc gttcggtctc
     1141 gtcgcgcagc acggcgacga tgaagtcgac gccgtggccg ggtccgacga tcaacgtcac
     1201 ggtgaacgcc accgacagcg tccgcccgtc gcggtggatc gccgggacct cgagcagacg
     1261 gtccccgtag gaggtctcgc cggtctccat gacccgctcg tagccggccc agtgccggga
     1321 acggagccgt gcggggatga tgaggtccag cgtctggccc cgcgcctcgg cacccggcca
     1381 gccgaagagc cgctccgcgg cgtcgttcca gaacacgatc gtgcctgccg gctccgcgat
     1441 gaccaccgcc tcggccacgt cgctccagag ccgggccagc tccgcttcgc caggcccggt
     1501 gcgcccggcg ccttcgttcg aagcggcggc caccgttccc gctaggcctg gatcatcccg
     1561 gccgcgacgg cgccgaccac cacgaggccg agtccggtct gggtgagacc gaggatgacg
     1621 gcgcgcggcg tcggcccgag ccgcagcagg cgctgcacga cgatcaaggc ggcaacggcg
     1681 accgcaccca cgacggcggc cggttcgagc gccacggcgg cggccgccag accgagggcg
     1741 atcgcgtcgc cggcccacac cgccgccggc tgccgaggcc gaccgtggag gccctggacc
     1801 agatctcgga cgttcacgat cgacgtcacc gcccgcgccg cgaggagcac ccagcacgcc
     1861 gcggcgatgc cggccggctg cccgtcggcc aagacgatca tggccgcgac cccggcgatg
     1921 ccgatggcgc cggcgagctc ggggaccagc cttcggcccc gcgaccgcat gtcgtaccag
     1981 agctctgcac cgaccagcgg agcgatgagg acgagcggca gccaggtgga gcccggagcc
     2041 tggagcaacg gcacggcggc gcagccggcc agcaccaggc cgtagcagcc gaccgacacg
     2101 ccggccatgc gggtgcgggg cagccagcgg cggcggtggc gatcgacgag caggaccttg
     2161 agcggcgtcc gggcgaggaa caagaccacg gccgccagcc ccaggccggc gccggaccag
     2221 ctcggcgcca cgaggagccc gagcaggacc ggctccagcg tcagccccca acctccgtgc
     2281 tcggtgggca cgagcaccga gcgcagggtc gatcgccgct gcggctggcc caccgggcga
     2341 gcggtcccgg aaggccgcgc gtccggacgc agctcggcgg cgcggcggct catgccagcg
     2401 agctcgtcac agccaagacg acgatcagca ccgcgacggc ggtcaccaag atgatcgatg
     2461 cccgcatcgg atgggccgga cgtgcgtcgc ggaggcggtc ggggtcgatc ggctgcagca
     2521 tcagctcgcc gcctccgcgc ccggcgcggc atcgccggct cggaggcgct ccgccttggc
     2581 cgcgtcctcc acctgggtcg cccacacgac gccgccgacc atggcgccga ag
//
LOCUS       DATMFS010000047         3031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_71493, whole genome shotgun sequence.
ACCESSION   DATMFS010000047 DATMFS010000000
VERSION     DATMFS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71493"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VNQ33_00875"
     CDS             <1..553
                     /locus_tag="VNQ33_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ96677.1"
                     /translation="PKGIGVLAAREGVELAPILLGGGQERERRSGTPDVAGAVALGVA
                     LAATERDRITVVGRVRGQAERLADGLAEATGHHVRRTVPPRSTPGVANIVHLSCQEVD
                     SEPLLFLLDEAGVAASAGSSCASGALEPSHVVEALGVAPDWAPGALRLSLGWCTTDEE
                     IDHALAVIPAALAQLRPHAEPAA"
     gene            550..1590
                     /gene="mnmA"
                     /locus_tag="VNQ33_00880"
     CDS             550..1590
                     /gene="mnmA"
                     /locus_tag="VNQ33_00880"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWJ96678.1"
                     /translation="MKVLVMMSGGVDSSVAAALLRDQGHDVVGVTLKLWGGESDSGCC
                     SVSDVDDARRVADALGIVHHVFNFGDAFTTHVVAPYAADHGAGRTPNPCIECNRHIKF
                     AAVDERARALGFDAVATGHHARVAVRPDGTRRIARGADAAKDQSYVLHMLGQDQLART
                     LLPLGGMQKDEVRALAAELGLRTASKPDSQDVCFITKTTGRGSFLDRRIPLTPGRVVD
                     AEGHEVGTVAAVELVTVGQRKGLGLAGGGAPRYAVDVDVPSATVTVGSKRDLLVDEQR
                     AASLQWSAGPVSGPVLVQVSAHGTPAEATVTEGPDRTAVVRWAVPQRRVAAGQSVVAY
                     EADEVVAGAIAV"
     gene            complement(1976..2185)
                     /locus_tag="VNQ33_00885"
     CDS             complement(1976..2185)
                     /locus_tag="VNQ33_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96679.1"
                     /translation="MSASLPDGGRPDGFTHASIVADVASSSAPIPNRAPPAAPPLELS
                     DTAVSPPNSGLDTPNVICPGSAHAV"
     gene            2142..>3031
                     /locus_tag="VNQ33_00890"
     CDS             2142..>3031
                     /locus_tag="VNQ33_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWJ96680.1"
                     /translation="MKPSGLPPSGREADIVSLNGCCLGYSVAGVRLGLTSSGTVVAEA
                     GRDWVGDITGTGDVPPDRDASYRTLRVESTRPVTGGAWHHVVAVYDEVSKSVWVSVDG
                     FAVGEGRDTFSGLEWSSIPHQSPHWMGIGLPVQGVTEPFSGAVDEVAVYDGALSLADI
                     RSDFLASGQSAPGGWNVPEGVTSGTEVLGPNAAKDWDDLLRCRCVDPVDTGSGNLHMP
                     IPAVSVPGRGPGLEIEMGYNSLAAATAGSIGYGWSTTLDMRLVDSGFTGRKHVVQETG
                     ATVPFDPDGSGWAAAPGYTAT"
BASE COUNT          374 a          975 c         1225 g          457 t
ORIGIN      
        1 cccgaagggc atcggcgtgc tggcagcgcg ggagggcgtc gagctggcac cgatcctgct
       61 gggtggcggc caggagcggg agcggcggag cggcacgccc gacgtcgccg gtgccgtcgc
      121 cctcggcgtg gcgctcgccg ccaccgagcg cgaccgcatc accgtcgtcg gccgggtgcg
      181 gggccaggcc gagcggctgg ccgacgggct ggccgaggcc accggccacc acgtccgccg
      241 gaccgtcccg ccccgctcga cgccgggcgt cgccaacatc gtccacctgt cgtgccagga
      301 ggtggacagc gagccgttgc tgttcctgct cgacgaggcc ggtgtagccg cctcggccgg
      361 ctcgtcgtgc gcgtcgggtg cgctggagcc gtcgcacgtg gtcgaggcgc tgggcgtggc
      421 gccggactgg gcgccgggcg cgctgcgcct gtcgctcggg tggtgcacca ccgacgagga
      481 gatcgaccac gcgctggcgg tcatccccgc tgcgctcgcc cagctgcgcc cgcacgcgga
      541 gccggcggcg tgaaggtcct cgtgatgatg agcggcgggg tcgactcgtc cgtcgctgcc
      601 gccctcctgc gcgaccaggg ccacgatgtc gtgggcgtca ccctcaagct ctggggcggc
      661 gagagcgact cggggtgctg ctcggtcagc gacgtcgacg acgcccgccg ggtggccgac
      721 gccctcggga tcgtccacca cgtcttcaac ttcggcgacg ccttcaccac ccacgtcgtc
      781 gccccctacg ccgcggacca cggcgccggc cgcacgccca acccgtgcat cgagtgcaac
      841 cggcacatca agttcgccgc cgtcgacgag cgggcccggg ccctgggctt cgacgccgtc
      901 gccaccggcc accacgcccg cgtcgccgtc cgccccgacg gcacccgccg catcgcccga
      961 ggcgcggatg ccgccaagga ccagagctac gtcctccaca tgctcggcca ggaccagctc
     1021 gcccggacgc tcctgcccct cggtggcatg cagaaggacg aggtgcgcgc cctggccgcc
     1081 gagctcggcc tgcgcaccgc atcgaagccc gacagccagg acgtgtgctt catcaccaag
     1141 acgacgggcc gcggctcgtt cctcgaccgc cgcatcccgc tgacaccggg tcgagtcgtc
     1201 gatgcagagg gccacgaggt cggcacggtc gctgctgtcg agctggtcac ggtgggccag
     1261 cgcaagggcc tgggcctcgc cggcggcggc gccccccggt acgccgtcga cgtcgacgtg
     1321 ccgagcgcga ccgtgacggt cggctccaag cgcgacctcc tggtggacga gcagcgagcg
     1381 gccagcctcc agtggtcagc cggcccggtg agcggcccgg tcctggtcca ggtcagcgcc
     1441 cacggcaccc ccgctgaagc caccgtcacc gaaggcccag accgcaccgc ggtcgtgagg
     1501 tgggccgtgc cccagcgaag ggtcgccgcc ggccagagcg tcgtcgccta cgaagccgac
     1561 gaggtcgtcg caggagcgat cgcggtctga cgcgcatcgg ggactgagcc gggccgggtg
     1621 cgcaagcgcc cggcccgttc gcgtggggag cggcgtcggg gtttcgagac gattgggtct
     1681 tgctgttggc gggtggctct ggcacgatcg cgagcgcatt gttcggggga gggcggcggg
     1741 ggtcgtctgt ccggtttgcg aggggcggtt cggggatggc cgagggtcga tttcgttggc
     1801 tggtggtcgt ggtggcggtg gcggtggtgt cgtcgttggt ggtgctgggg gcgcggcctg
     1861 tttcggctac ggcggcctat gaggatgtga tcgagggaag gcatccggtg aggtatctgc
     1921 ggttgggcga gtcggatggt tcgacggacc cggtcgatga gatgggttcg acgggtcaga
     1981 cggcgtgggc ggatccgggg cagatcacgt tcggtgtgtc gagtccgctg ttcggtgggg
     2041 atacggcggt gtcgctgagc tcgagcggtg gtgccgcggg tggggcgcgg ttcgggatcg
     2101 gggcgctcga tgatgcgacg tcggcgacga tcgaggcgtg ggtgaagccg tcgggcctgc
     2161 cgccgtcggg gagggaggcg gacatcgtgt cgctcaacgg ctgctgtctc ggctactcgg
     2221 tggccggggt gcggttgggg ttgacgtcgt cggggacggt ggtggcggag gcgggccggg
     2281 actgggtggg tgacatcacg ggcacgggtg atgtgccgcc ggatcgtgat gcgtcgtatc
     2341 ggacgctgcg ggtcgagtcg actcggccgg tgacgggtgg ggcttggcac catgtggtgg
     2401 cggtgtacga cgaggtgtcg aagtcggtgt gggtgtcggt ggacgggttc gcggtgggtg
     2461 agggccgtga caccttctcg ggtctggaat ggagcagcat cccgcaccag tcgccgcact
     2521 ggatgggcat cgggctgccg gtccagggcg tgacggagcc gttctcaggt gcggtggacg
     2581 aggtcgccgt gtacgacggt gcgctgtcgc tggctgacat ccgcagcgac ttcttggcgt
     2641 cggggcagtc ggcgccggga ggctggaacg tcccggaggg cgtgacgagc ggcacggagg
     2701 tgctgggtcc gaacgcggcg aaggactggg acgatctgct gcgctgccgg tgcgtcgatc
     2761 cggtggacac gggatcgggg aacctgcaca tgccgatccc ggcggtgtcg gtgccgggcc
     2821 ggggccccgg gctggagatc gagatggggt acaactcgct ggcggcggcg actgcgggga
     2881 gcatcggcta cggctggtcg acgacgctcg acatgcgcct ggtcgattcg gggttcaccg
     2941 ggcgcaagca cgtggtgcag gagacggggg caacggtccc gttcgatcct gacgggagcg
     3001 gctgggcggc tgcgcccggc tacacggcga c
//
LOCUS       DATMFS010000048         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_71519, whole genome shotgun sequence.
ACCESSION   DATMFS010000048 DATMFS010000000
VERSION     DATMFS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71519"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..875)
                     /locus_tag="VNQ33_00895"
     CDS             complement(36..875)
                     /locus_tag="VNQ33_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWJ96681.1"
                     /translation="MAEQPELETLRRDLDRDIGGKRIKTVEVPGSGAIPRHTNKKQFV
                     SLLEGAKVTSVVRRDLYLVTKLDSGDALVIDLQNGGQLRRQAAKEEVDKRTKVSITFT
                     QGGQLRAIDDTGNLELFVVPFDQLTEAVPELADLGFDILDEPVSWTSFGERMLRHRDV
                     KLRALLLNRSVIAGIGPVYADEILHASGLRPDREVSSLSTQEIRRFYRAVVETLHEAA
                     KHRGVTTSDGQYSDLAGKPGGWTDELQVFERDGKACRRCRGVVSKVRMSAKTVYLCED
                     CQV"
     gene            complement(868..1689)
                     /gene="rnc"
                     /locus_tag="VNQ33_00900"
     CDS             complement(868..1689)
                     /gene="rnc"
                     /locus_tag="VNQ33_00900"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWJ96682.1"
                     /translation="MTAEPGVDAGAAEQLRAPASPSVDAPGPPALEFHPIPPKALAAF
                     CDRLEYRFGDPELLGQALTHRSWCAEHAGYRSNERLEFLGDAVLGLVVTDDLYALLPE
                     AGEGQLSRMRAAVVCSPALAEMAAELSLGEVLLLGKGEHASGGRERPSILADAMEAVI
                     GAVYLDGGPTPAADLVSRLVAPRLSSAGAPDHKSRLHELAASDPQRSVVFEITEQGPE
                     HDKTFRARVVLDGRPLGVGVGRSKKQAEQAAARETWAQLDREPGDLPAREEATHG"
     gene            complement(1692..1955)
                     /locus_tag="VNQ33_00905"
     CDS             complement(1692..1955)
                     /locus_tag="VNQ33_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWJ96683.1"
                     /translation="MSRQDVFELVRDRLADILETDPSGIKEGDSFADDLGADSLALIE
                     LVEELEEELGERSVGFRIEDEDLEDLKTVRDAVDYVFDKLGGN"
     gene            2145..>2787
                     /locus_tag="VNQ33_00910"
     CDS             2145..>2787
                     /locus_tag="VNQ33_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWJ96684.1"
                     /translation="MDESTEHDTPTIEAPRKGWRISRRSAEEKAASAPTPAADPVDEL
                     TEPEGAPDEEVTKDQPTPRELAVAEAERHLDGPALTVPADAPSPADLEATWSDEVLDA
                     LEVLRADLGGRRTGLAISGGGSLGSFEAGVLRFLYDHAAIAPVAVAGNSAGGLNAAKL
                     AEGDRPDGTRAIDEVERVWRSLRTTDDMWKPEPWLTRLQASASWASTWRGYLGD"
BASE COUNT          414 a          976 c         1001 g          396 t
ORIGIN      
        1 gagcctgccg ccccaggggc gcgagtcccc gcgggtcaga cctgacagtc ctcgcacagg
       61 tagacggtct tggcgctcat gcggaccttc gacaccacgc cgcggcagcg tcggcacgcc
      121 ttgccgtcgc gctcgaagac ctggagctcg tcggtccagc cgccgggctt gccggcgagg
      181 tccgagtact ggccgtcgga ggtggtgacg ccacggtgct tggccgcttc gtgcagggtc
      241 tcgaccacgg cgcggtagaa gcggcggatc tcctgggtgg agagcgagga gacctcccgg
      301 tccggccgga gacccgacgc gtggaggatc tcgtcggcgt agacggggcc gatgccggcg
      361 atgacgctgc ggttcaacag gagcgcccgc agcttcacgt cgcggtggcg cagcatgcgc
      421 tcgccgaacg aggtccacga gaccggctcg tcgaggatgt cgaagccgag gtcggccagc
      481 tcgggcacgg cttcggtcaa ctggtcgaac ggcaccacga agagctcgag gttgccggtg
      541 tcgtcgatgg cacggagctg gccgccctgg gtgaaggtga tggagacctt ggtccgcttg
      601 tcgacctcct ccttggcggc ctggcgccgc agctgcccgc cgttctgcag gtcgatcacc
      661 agcgcgtcac cggagtccag cttggtgacg aggtagagat cccggcgcac gacgctggtg
      721 acctttgctc cttcgagcag ggagacgaac tgcttcttgt tcgtgtggcg cgggatggct
      781 ccagagcccg gcacctcgac ggtcttgatc cgcttgccac cgatgtcgcg gtcgagatcc
      841 cgtcgcaggg tctcgagttc cggctgctca gccatgggtt gcttcctctc gtgcggggag
      901 atccccgggt tcgcggtcca gctgcgccca ggtctcccgg gcggcggcct gttccgcctg
      961 cttcttcgat cgtccgacgc cgacgcccag agggcgtccg tcgagcacca cgcgggcgcg
     1021 gaaggtcttg tcgtgctccg gcccctgctc ggtgatctcg aagaccacgg agcgctgggg
     1081 gtcgctggcc gccagctcgt ggagccggct cttgtggtcc ggtgcgccgg cgctcgacag
     1141 ccgcggcgcc accaggcggc tcaccagatc agcggccggc gtgggcccgc cgtcgaggta
     1201 cacggcgccg atcacggcct ccatggcatc ggccaggatc gacggccgct cacggccacc
     1261 cgaagcgtgc tcgcccttgc ccagcagcag cacctcgccc agggacagct cggcggccat
     1321 ctcggcgagc gccggcgagc acaccacggc ggcgcgcatg cgggagagct ggccttcgcc
     1381 ggcctccggc agcagcgcgt acaggtcgtc ggtgaccacg aggccgagca cggcgtcgcc
     1441 caggaactcg aggcgctcgt tcgaccggta gccggcgtgc tcggcgcacc acgaccggtg
     1501 cgtgagcgct tgcccgagca gctcggggtc gccgaaccgg tactccagcc gatcgcagaa
     1561 ggcggcgagc gccttcggcg ggatcgggtg gaactccagg gcggggggcc caggcgcgtc
     1621 gaccgacggc gacgccggcg cgcggagctg ctcggcggcg ccggcgtcga cgcccggctc
     1681 ggcggtcacg cctagtttcc gcccagcttg tcgaacacgt agtcgacggc gtcgcggacg
     1741 gtcttcaggt cctcgaggtc ctcgtcttcg atccggaacc cgaccgagcg ctcgcccagc
     1801 tcctcctcga gctcctcgac gagctcgatg agcgcgaggg agtcggcgcc caggtcgtcg
     1861 gcgaaggagt ccccttcctt gatgccggag ggatcggtct cgaggatgtc ggcgaggcgg
     1921 tcgcgcacga gctcgaacac gtcctggcgg ctcatcgggt cctgtccgac atgggtttca
     1981 gcgggcacag tgcactcctg atcgtgaaag gggcggtggc gactgtagcg ggtggaaaac
     2041 tttgtgcgct cgtgaacgac gggacgcggg aggaatcggc acgcgagcgg catgatgggt
     2101 ccgacggacc gacgacgagg gcctgatctg ccccaggaaa cgacatggac gagtccaccg
     2161 agcacgacac cccgaccatc gaagcacccc gaaaaggatg gaggatcagt cgccgctccg
     2221 ccgaggagaa ggccgccagc gcgccgacgc cagcagccga tccggtggac gagctcaccg
     2281 aacccgaggg agcacccgac gaggaggtga caaaggacca gcccacgccc cgcgagctcg
     2341 ccgtagccga ggccgaacgc cacctcgacg gaccggcgct caccgtgccc gccgacgccc
     2401 cttcacccgc cgatctggag gccacctgga gcgacgaggt gctcgacgcg ctcgaggtgc
     2461 tccgggcgga cctcggcgga cgccgcaccg gcctcgccat cagcggcggc ggttccctcg
     2521 gcagcttcga ggccggcgtg ctgcggttcc tctacgacca cgccgcgatc gcccccgtcg
     2581 ccgtggccgg caactcggcc ggcggcctca acgccgccaa gctcgccgaa ggagaccgcc
     2641 ccgacgggac ccgggccatc gacgaggtcg aacgggtgtg gcgctcgctg cggaccaccg
     2701 acgacatgtg gaagcccgag ccgtggctca cgcgcctgca ggcgtcggca tcgtgggcct
     2761 ccacctggcg gggctacctc ggcgatg
//
LOCUS       DATMFS010000049         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_164043, whole genome shotgun sequence.
ACCESSION   DATMFS010000049 DATMFS010000000
VERSION     DATMFS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164043"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VNQ33_00915"
     CDS             complement(<1..467)
                     /locus_tag="VNQ33_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ96685.1"
                     /translation="MTDGTTPAIELRDLRKSFGSNEVLKGIDLAVAPKQVVCVIGASG
                     SGKSTLLRCVNRLEEPTGGQVLIDGIDITHPDCDLDLVRRHIGMVFQQFNLFPHLKAL
                     DNVTLAQREVLGRSKQEAEEVGQEMLDRVGLAEKAGSYPSQLSGGQQQRVAIAR"
     gene            complement(464..1324)
                     /locus_tag="VNQ33_00920"
     CDS             complement(464..1324)
                     /locus_tag="VNQ33_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWJ96686.1"
                     /translation="MTDPTVERLGEGEETLPELYRSGRAKQRRSRNSRIAAYTLGALI
                     VAAVVVTADWERIGDKYFNGQIARDMFPDVITIAAKNTVVYTVFSFTGGVVLGLSMAL
                     LRLSSIRVYRSFAATYIDIFRGLPALLTIILVGFMTPIALGIHFPTLFGVPTGGIVAL
                     SLVAGAYLAETIRSGIEAVPKGQVEAARSLGMTHAKTLRKVVIPQAFRIIIPPLTNEL
                     VLLLKDTSLLAILGVTITQKELTKFAQSESSRTFNGTPLVVAGAIYLIITIPLTRLVS
                     QLEKRNKAMR"
     gene            complement(1357..2220)
                     /locus_tag="VNQ33_00925"
     CDS             complement(1357..2220)
                     /locus_tag="VNQ33_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWJ96687.1"
                     /translation="MNTLRKHHLVAFGLAGALLFGAAACGSSDSGSDGGTKSTTTVKP
                     TEDGDATPKLDALAGKRIGVQSGTTGLTYANEHKPDGAEIVEFSDTDGLFGALSSGDI
                     DAILQDLPVNAGRAAEDDSVEVVETYATDEQYGFAVEKGSDLKGELDDALAEVKDDGT
                     YDEIYAKYFPKAGEDAGPGPAASDVKGSKTITVCSDIPYAPMEFEGEGPRGLQYTGFD
                     IDLLDAMAVKMDANLKVIDSEFDGILGNLSAGTCDVVASSLTITPDRQKEVDFSEPYF
                     DADQSLLVPVS"
     gene            2390..>3532
                     /locus_tag="VNQ33_00930"
     CDS             2390..>3532
                     /locus_tag="VNQ33_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ96688.1"
                     /translation="MLRALIEEWDEDLRSGDPLGFPGYAEKVAAMDGESVRTGLADGF
                     VLVECDFSVIGGSMGLVHGEKVVRAFDRAVERRLPVVVVTRSGGARMQEGMLSLIQLS
                     RTAAAVNRHHAAGLLSVAVHRSPTTGGVFASYGSLTDLRIAEAGAMIGFAGPRVVEQT
                     SGRTVEGTSHAADTALAHHIVDAVVDPGEALAWVRVALGLEDRPLRPSRAPVPAKQPA
                     PPPPGTDPAWAAVLTSRQLGRPSGMQVAAAATTSWTELGEGTDPSMRVGLATVCGRRA
                     VVIAMDRYAGAGQPEPAGYDLAGRGIALAGRLGIPIVTFVDTPGAALSPEAENHGIAR
                     AIANTHAAMSATPVPTVSVCVGEGGSGGAQALSAADRLLIQDGAIFS"
BASE COUNT          491 a         1115 c         1352 g          574 t
ORIGIN      
        1 cgggcgatgg ccacccgctg ctgctggcct cccgagagct gggacgggta ggagccggcc
       61 ttctccgcga gcccgacccg gtccagcatc tcctgcccga cctcctcggc ctcctgcttg
      121 gagcgtccga ggacctcgcg ctgggcgagg gtgacgttgt cgagcgcctt gaggtgcggg
      181 aagaggttga actgctggaa gaccatcccg atgtggcgac ggacgaggtc caggtcgcag
      241 tcggggtggg tgatgtcgat gccgtcgatc agcacctgcc cgcccgtggg ctcctcgagc
      301 cggttgacgc agcgcaggag cgtggacttg ccggagccgg aggcgccgat gacgcacacg
      361 acctgcttcg gggcgacggc gaggtcgatg cccttcagga cctcgttgga tccgaacgac
      421 ttccgcaggt cgcggagctc gatggcgggc gtggttccgt cggtcatcgc atggccttgt
      481 tgcgcttctc gagctgggac acgaggcggg tgagcgggat cgtgatgatc aggtagatgg
      541 cgccggcgac cacgaggggg gtgccgttga aggtgcggga cgactcgctc tgggcgaact
      601 tcgtcagctc cttctgcgtg atcgtcacgc cgaggatcgc gagcagcgag gtgtccttca
      661 acagcagcac cagctcgttg gtgagcggcg ggatgatgat gcggaacgcc tgcgggatga
      721 cgaccttgcg cagcgtcttg gcgtgggtca tccccagcga gcgcgccgct tcgacctggc
      781 ccttgggcac cgcctcgatg ccagaccgga tcgtctccgc caggtacgcg ccggcgacga
      841 gcgagagggc cacgatgccg cccgtcggca caccgaacag ggtggggaag tggatgccca
      901 gggcgatggg cgtcatgaag ccgacgagga tgatcgtgag cagcgccggc aggccgcgga
      961 agatgtcgat gtaggtcgcg gcgaacgagc ggtagacccg gatcgagctc aggcgcagga
     1021 gcgccatgct gaggccgagg accaccccgc cggtgaacga gaagaccgtg tagacgacgg
     1081 tgttcttggc ggcgatggtg atcacgtccg ggaacatgtc ccgggcgatc tgcccgttga
     1141 agtacttgtc gccgatgcgc tcccagtcgg ctgtgacgac gacggcggcg acgatgaggg
     1201 cgccgagggt gtaggcggcg atgcgggagt tgcgagagcg ccgctgcttg gcgcgcccgg
     1261 atcggtacag ctcggggagg gtctcctcac cctccccgag ccgttcaacc gttggatcgg
     1321 tcatggatca gcggtggtgg cttctggtcc tggtgctcag ctgaccggga ccaggagcga
     1381 ctggtcggcg tcgaagtagg gctccgagaa gtcgacctcc ttctggcggt cgggggtgat
     1441 ggtcaacgag gaggcgacca cgtcgcaggt gccggccgag aggttgccga ggatgccgtc
     1501 gaactccgag tcgatgacct tgaggttggc gtccatcttc accgccatcg catcgagcag
     1561 gtcgatgtcg aagccggtgt actgcaggcc gcgagggccc tcgccctcga actccatggg
     1621 ggcgtagggg atgtcggagc agaccgtgat ggtcttcgag cccttgacgt cggacgccgc
     1681 cggaccgggt ccggcgtcct cgccggcctt cgggaagtac ttggcgtaga tctcgtcgta
     1741 ggtgccgtcg tctttcacct cggccagcgc gtcgtcgagc tcgcccttca ggtcgctgcc
     1801 cttctcgacg gcgaagccgt actgctcgtc ggtggcgtag gtctcgacca cctcgaccga
     1861 gtcgtcctcg gcggcacggc cggcgttgac cggcaggtcc tggaggatgg cgtcgatgtc
     1921 acccgaagaa agggcgccga agaggccgtc ggtgtcgctg aactcgacga tctcggcgcc
     1981 gtcgggcttg tgctcgttgg cgtaggtgag gcccgtggtg ccggactgga cgccgatgcg
     2041 cttgccggcg agggcgtcga gcttcggggt ggcgtcgccg tcctctgtcg gcttcaccgt
     2101 ggtggtgctc ttcgtcccgc cgtcggaacc ggagtcggag ctgccgcagg cggcggcacc
     2161 gaagaggagc gctccggcga ggccgaatgc gacgaggtgg tgcttgcgga gggtgttcac
     2221 gctgggtctc ctgggtcgtg gtcgccaggc gaccggaacc cgggcatcgt acgcatctgt
     2281 cggtttcggc gctgtgaaca cgtgaacgcg acgggaacga cggggggacc ggccgatcgt
     2341 ggcggatccg gttgccggtc gtgacctggg ggtctggcag gctggaccga tgctgagggc
     2401 gttgatcgag gagtgggacg aggacctgcg aagcggtgat ccgctggggt tcccgggcta
     2461 cgccgagaag gtcgcggcga tggacggcga gtccgtccgg accgggttgg ccgacggctt
     2521 cgtgctcgtc gagtgcgact tctcggtcat cggtgggtcg atgggcctgg tgcacgggga
     2581 gaaggtggtg cgggcgttcg accgggcggt cgagcggcgc ctgccggtgg tggtggtcac
     2641 ccggtcgggc ggagcccgca tgcaggaggg gatgctgtcg ctgatccagc tgtctcgcac
     2701 cgcggccgcc gtcaaccgcc accacgccgc cggcctgttg tccgttgcgg tccaccggtc
     2761 gccgaccacg ggcggggtgt tcgcgtcgta cggctcgctc accgatctcc ggatcgccga
     2821 ggccggcgcc atgatcggct tcgccggtcc gcgggtcgtc gagcagacct cgggccgcac
     2881 ggtcgagggc acctcgcacg cagccgacac cgcgctggcg caccacatcg tcgatgcggt
     2941 ggtcgatccc ggggaagccc tcgcgtgggt ccgggtcgcg ctcggcctgg aggatcgtcc
     3001 gctgcgaccg agccgtgcac cggtcccggc caagcagccg gcaccgccgc ctccgggcac
     3061 cgatccggcc tgggccgccg tgttgacctc gcggcagctg ggacggccga gcggcatgca
     3121 ggtcgcggcg gccgccacga cgagctggac cgagctgggg gagggcaccg acccgtcgat
     3181 gcgggtcggg ctcgccacgg tgtgcgggcg gcgggcggtc gtcatcgcca tggaccgcta
     3241 cgcgggggcc ggccagcccg agccggcggg ctacgacctg gccggccggg gcatcgccct
     3301 ggcgggtcgt ctcggcatcc cgatcgtcac gttcgtcgac acaccgggcg cagcgctgtc
     3361 gccggaagcc gagaaccacg gcatcgctcg cgccatcgcc aacacccatg ccgccatgtc
     3421 cgccacaccg gtgccgaccg tctcggtgtg cgtgggcgag ggcgggagcg gcggggcgca
     3481 ggcgctctcg gccgcggacc ggctgctcat ccaagacggt gcgatcttct cg
//
LOCUS       DATMFS010000050         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_48097, whole genome shotgun sequence.
ACCESSION   DATMFS010000050 DATMFS010000000
VERSION     DATMFS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48097"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="VNQ33_00935"
     CDS             <1..1896
                     /locus_tag="VNQ33_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96689.1"
                     /translation="VAAGLLAQLPVLCLLDAVDATVWQAQLAVVAQAFAVLVVTDRVE
                     VGRWVRRVAAAWAVTIAALFTAATTLDSTVGRLFTDAVADLHRGPTGLCLAAAALLAG
                     HVAWLRAESDEIRTLSLVSATALGLAAMWFGSVDAVGSQTALGLVAVAAALVMLAGRQ
                     APTEWRGAPVITAGVIGGVAVLPLLGAVASMLVAASAVSAETWHRSGATVAADLQLEE
                     AVDYGARSLALQLTAVAVGVVALVRRSARVAIGTSAAVVAVLALVLSPLLVPLTITAT
                     VLIAVAAVAVGVAAAFGTGARTPGFVVATGFAVAAYAWATPWSLATPGLTLLTLSVGI
                     AGALVVAAIARRDEAVQAAGAATAWVVAATPLLVGLAVWEGGVSTAMSWALAATAAAV
                     LSVVGVVLLDPKGTGPAPARVMARTAEATALVGYLGGLLGAVGSADPNAASVALAAGV
                     IGFGLQALRPGRLAAGAAAAVELLALVWLQLDQAGVVTVEAYTLPLAAVLLAAGLVGA
                     RLHRAEDGEPASWVTCGPALVVAFAPTVWLSFVEPGSLRPLVGLVAGALVLVGGAVWG
                     KRALVDVGAAAVIALGLQQIAPVVGEVPNWATIGATGILLLAVGATFEQRRRDVKAVL
                     RSYSALT"
     gene            complement(2192..>3285)
                     /locus_tag="VNQ33_00940"
     CDS             complement(2192..>3285)
                     /locus_tag="VNQ33_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HWJ96690.1"
                     /translation="DAGIAWSDVDFVSGADTMRNGYPGYVSGATFAQALGWTGARVAS
                     SYAACASGVTALEAARTRILAGLADVALVVGADTTPKGFLAPNAGDRPTDPDWLRFRL
                     VGATNPTYFALAARRRMDLYGATSEDFAKVKVKNAKNGLANPNARFQKEVSVDDVLAS
                     PMVADPLHLLEICATSDGGAAVILTSMDFARRHAGSAGPPVRVAGVSTVTPTYPNTII
                     DLPDMATDSTAVVARDERTFKQSIAHAAYEEAGIGPDDLSLAEVYDLSTALELDWYED
                     IGLCKQGEAERLLNDGDTQLGGRIPVNPSGGLACFGEAVPAQAIAQICEVTWQLRGQA
                     TGRQVEGATSGITANQGLFGHGSSVILVK"
BASE COUNT          329 a         1156 c         1291 g          509 t
ORIGIN      
        1 gtcgctgccg ggttgctcgc tcagctcccc gtgctgtgcc tgctcgacgc ggtcgacgcg
       61 acggtgtggc aggcgcagct ggcggtcgtg gcccaggcct tcgcggtgct cgtggtgacc
      121 gaccgcgtcg aggtcgggcg ctgggtccgg cgggtggcag cggcatgggc ggtgaccatc
      181 gccgcgctgt tcacggccgc cacgaccctg gactcgaccg tcggtcgcct cttcacggac
      241 gcggtggccg acctccaccg cgggccgacg gggctgtgtc tggccgcggc ggcgctgctg
      301 gccggacacg tcgcctggct ccgagcggag tctgacgaga tccggaccct ctccctcgtc
      361 agcgcaacgg cgctcgggct ggcggcgatg tggttcgggt cggtcgatgc ggtcggttcg
      421 cagaccgctc tcgggctcgt ggccgtcgcc gccgccctcg tcatgctggc tggccgtcag
      481 gccccgacgg agtggcgcgg cgcacccgtg atcaccgcgg gcgtcatcgg gggcgtggcg
      541 gtcctgccgc tgctcggtgc ggtggcatcg atgctggtgg cggcctcggc ggtgtcggcc
      601 gagacctggc accgctccgg cgccacggtg gccgccgacc tccagctcga ggaggcggtg
      661 gactacggcg cccgctcctt ggccctccag ctcacggccg tcgccgtggg cgtggtcgcc
      721 ctggtgcgcc gaagcgcccg ggtggcgatc ggcacgtcgg ctgcggtggt cgccgtcttg
      781 gccctcgtcc tcagcccgct gctggtcccg ttgacgatca cggcgacggt gctgatcgcc
      841 gtggccgcgg tggcggtcgg cgtggctgct gcgttcggca ccggggctcg gaccccaggg
      901 ttcgtggtgg cgacgggctt cgccgtggcg gcctacgcct gggccacccc gtggtcgctc
      961 gccacgccgg ggctcacctt gctgacgctc tcggtgggca tcgccggcgc gctcgtcgtg
     1021 gccgccatcg cccggcggga cgaggcagtc caagccgcgg gcgcggccac ggcctgggtg
     1081 gtcgccgcca cgccgctgct ggtgggcttg gccgtgtggg agggcggggt cagcacggcc
     1141 atgtcgtggg cgctcgccgc cacggccgct gccgtgctga gcgtcgtcgg ggtcgtgctg
     1201 ctcgacccga agggcacggg tccggcccca gcgcgggtga tggcccgtac ggccgaggcc
     1261 acggcgctcg tcggatacct gggcggcttg ctcggtgctg tcggctcggc cgatccgaac
     1321 gccgcgtcgg tggccctggc tgccggggtg atcgggttcg gtctccaggc cctgcgcccc
     1381 ggtcggctcg ccgccggtgc cgctgcggcc gtcgagctgt tggcgctggt ctggctccag
     1441 ctcgaccagg ctggcgtcgt caccgtcgag gcgtacacgc tcccgctcgc cgcggtgctc
     1501 ctcgccgccg ggctcgtcgg cgcccggctc caccgggccg aagacggcga gccggcctcc
     1561 tgggtcacct gcggcccggc cctggtcgtg gcgttcgcgc cgaccgtctg gctctcgttc
     1621 gtcgagcccg gctcgctccg tccgctcgtc gggctcgtcg ctggtgcact cgtgctggtg
     1681 ggcggcgccg tctggggcaa gcgcgcgctg gtcgacgtgg gcgcggcggc ggtgatcgcc
     1741 ctcgggctcc agcagatcgc tcccgtggtg ggcgaggtcc cgaactgggc gaccatcggc
     1801 gccaccggga tcctgctgct cgccgtcggc gcgacgttcg agcagcggcg gcgggacgtg
     1861 aaggccgtcc tccgcagcta ctcggccctc acgtgacgcg ggccgtcggg catcgcccgg
     1921 agtgctgacc tcgcggcctg ctggtcatca ggcggcggtg ggtgtggtgg tcgtgcgcgc
     1981 agcacggcca ccgcaccggc gcgacgatgg ggcgatgagc gatgtgcacc actgcccctt
     2041 ctgcgagctt cggttcgtca acctggccga gctgaacttc cacatccagc tcgaccaccc
     2101 tgatcgggac gtgcccgaac gggagtactg accgggccgg cccgggccgg cggcgaggcc
     2161 ggcccgctcc cgggctcggc cgccgcgacg gctacttgac gaggatgacc gacgagccgt
     2221 ggccgaacag gccctggttg gcggtgatgc ccgacgtggc cccctcgacc tggcggcccg
     2281 tggcctggcc gcggagctgc caggtgacct cgcagatctg ggcgatggcc tgggccggga
     2341 cggcctcgcc gaagcaggcg agcccgccgc tcgggttgac cgggatgcgg ccgccgagct
     2401 gcgtgtcgcc gtcgttgagc agccgctcgg cctcgccctg cttgcacagg ccgatgtcct
     2461 cgtaccagtc gagctcgagc gccgtcgaca ggtcgtacac ctcggcgagc gacaggtcgt
     2521 cggggccgat gccggcctcc tcgtacgcag cgtgggcgat cgactgcttg aaggtccgct
     2581 cgtcgcgggc caccacggcc gtcgagtcgg tggccatgtc gggcaggtcg atgatcgtgt
     2641 tcgggtaggt gggcgtcacc gtcgacacgc cggcgacgcg gaccggtggc cccgccgagc
     2701 cggcgtggcg gcgggcgaag tccatcgacg tgaggatcac cgcggcgcca ccgtcggacg
     2761 tggcgcagat ctcgagcagg tgcagcgggt cggccaccat cggcgaggcc agcacgtcgt
     2821 cgaccgacac ctccttctgg aagcgggcgt tggggttggc caggccgttc ttggcgttct
     2881 tgaccttgac cttggcgaag tcctcgctgg tggcgccgta gaggtccatg cggcggcggg
     2941 cggcgagggc gaagtaggtg gggttggtgg cgcccaccag gcggaagcgc agccagtccg
     3001 ggtcggtggg ccggtcgccg gcgttggggg cgaggaaccc cttcggggtg gtgtcggcac
     3061 cgaccacgag cgccacgtcg gccaggccgg cgaggatgcg ggtccgggcg gcctcgagcg
     3121 cggtcacgcc ggatgcgcag gcggcgtagg acgacgccac ccgggcgccg gtccagccga
     3181 gcgcctgggc gaacgtggcg ccggagacgt agccggggta gccgttgcgc atggtgtcgg
     3241 cgccggacac gaagtccacg tcgctccagg cgatgccggc gtcgc
//
LOCUS       DATMFS010000051         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_2239, whole genome shotgun sequence.
ACCESSION   DATMFS010000051 DATMFS010000000
VERSION     DATMFS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VNQ33_00945"
     CDS             complement(<1..333)
                     /locus_tag="VNQ33_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWJ96691.1"
                     /translation="MSRASEESNRRMLRARDAMDRHFAEPLDVPAVAAIAHTSEAHFS
                     RTFKATFGESPHRYLQRRRVERAMFLLRSTDRSITDICLDVGFGSLGTFSRTFRAIVG
                     EPPSAYRER"
     gene            496..1038
                     /locus_tag="VNQ33_00950"
     CDS             496..1038
                     /locus_tag="VNQ33_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ96692.1"
                     /translation="MRRDRRAQSCVCTSEAGGDVGLRTTRRAGEPGLDRGRDRDRRRA
                     EELVQDAFEQCYRRWRRVSQLDRPGAWVRRVAINAAVSSMRKRASERRAIERLSARAT
                     SDGASADPLDALADAVLWTEVRSLPGEQAAAIALRYVADLSITEVADTLEISPTAAKS
                     LLHRGRRTLRTAPSLETYAE"
     gene            1052..3535
                     /locus_tag="VNQ33_00955"
     CDS             1052..3535
                     /locus_tag="VNQ33_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HWJ96693.1"
                     /translation="MNAPHDDHGDDDPDADAHDPLLAAAGARLRADAHSLSADAIQLA
                     VLHRRNRRLSAGLAAALVAILAIGAGTVALRNGDGSSNDLASNVKPVTADQRGKVEDL
                     VARLAKKQPVDPRTVKLVSSVSQFADCDALIGDLRTVGAEHVGSRGFGANTGEVGFSA
                     EMRSISKLDAAADSMRLTPYGPVASVANGGETLGTNVQVTGVDELDSVKAVGPLIYDL
                     DGKGNLRITDGATLQVRSTLDVTPKAIGAPDKPKKDDPQPPPTSVSRLLVAGERVVIF
                     GSESEISEPVKGDPSATRAETSYLTVTFVDATDIAKPKVTDRVRIEGGLVSARMVDGE
                     VRLVTTSNMADLGFVMPTTPTSVAKALEQNRRSVASSTAADWIPDWQRASGEPQPLVP
                     CDRVYVPDTFAGVAMTSMVTFPAGASRFEPAGTSILAPGSTLYAGLDKVAISSEVWVD
                     PIDQERLEFDDWRTAVHEFRFSPGHAPGYEGSGIVDGSTIGQFAFGELGDSIAVVTSR
                     GTPWSQDTTKLGVDLTVLTPGEKGRLDVASKVKDLSDGKGEVTAVRFVTDRVLVSTGL
                     FGRQIIVIDVTDPTAPRRAGGVVVPGDVGYFHPLPDGRALVVGSRSDTVSIGKDKVSR
                     SWVQAHLLDVTDADAPKLVATWERPWSADDVGSDHHAFTYWPNRKLAMWGISDTNWSQ
                     RDPNPNHAVVLDTDGGVQVALPVANQPNEVAPPCPSVPVPDDAQSMVGPDTIVLRCDD
                     GATTSVDWPRYECSKVDKQLVARFVPEDQHEKAFFLCNPAPLPRVTRVLVVAGRPILL
                     TDQTLEALDPKTFASTAIAYHPSSGAFFS"
     gene            complement(3615..>3898)
                     /locus_tag="VNQ33_00960"
     CDS             complement(3615..>3898)
                     /locus_tag="VNQ33_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ96694.1"
                     /translation="AADPAGVTYWTKTLDTKRRTRGTVVVGFSESSEFRRKQVENTDL
                     AVDYICLLGRAPTTAEAADWTVRQKAGTTQAELAMELLDSAAYATHILG"
BASE COUNT          605 a         1425 c         1319 g          549 t
ORIGIN      
        1 ccgttcgcgg taggcggacg ggggttcgcc gacgatggcg cggaacgtcc ggctgaaggt
       61 tccgaggctg ccgaagccga cgtccaggca gatgtcggtg atgctccggt ctgtcgagcg
      121 caggaggaac atggcccgct ccacccggcg ccgctggagg taccggtggg gagactcgcc
      181 gaacgtcgcc ttgaatgtgc ggctgaagtg ggcctccgat gtgtgggcga tggcggccac
      241 cgcgggcacg tccaacggtt cggcgaagtg ccggtccatc gcgtcgcgag cccgcagcat
      301 ccggcggttc gactcctccg atgcccggct caccccgcca tcagtagcac ccgccccgcc
      361 cgcgcccagg cggcgggcgc gtccctgtcg tcccgccagc tgacggttcg ttgttctcgt
      421 agacgagggt ccactcgccg tcggacacct ggccgatggc gaaccttgaa ccagcgccgt
      481 ccgtgaaacg cgaggatgcg gcgcgaccgc cgggcccagt cgtgcgtgtg tacctcggaa
      541 gcaggtgggg atgtcggact tcgcacaact cgtcgagcgg gagagccggg gcttgatcgc
      601 ggccgtgacc gcgatcgtcg ccgagccgag gagctcgtcc aggacgcgtt cgagcagtgc
      661 taccggcgct ggcgccgggt gtcgcagctc gaccgtccag gagcgtgggt acggcgagta
      721 gccatcaacg cggccgtctc gtcgatgcgg aagcgtgcga gcgagcggcg tgccatcgag
      781 cggctgagcg ctcgcgcgac ctcagatggg gcgtcggccg atccgctgga cgcgctcgcc
      841 gacgccgtct tgtggacaga ggtccgttcg ctccccggtg agcaggccgc cgccatcgcc
      901 ctgcgctacg tcgccgacct gagcatcacc gaggtcgccg acaccctcga gatctccccc
      961 accgcggcca agtccctcct ccaccgcggg cggcggacgc tgcgcacggc tcccagcctc
     1021 gagacctacg ccgagtgaaa gcaggctcac gatgaacgca ccccacgacg accatggcga
     1081 cgacgacccc gatgccgatg ctcacgatcc gctgctcgcc gccgccgggg ctcgcctgcg
     1141 ggcggacgcc cactcgctct cggccgacgc gatccagctg gccgtgctcc atcggcgcaa
     1201 ccgccgtctg agcgccggac tggccgcggc gctggtggcg atcctggcaa tcggcgcagg
     1261 caccgtcgcc cttcgcaacg gcgatggcag ctcgaacgac ctggcgagca acgtgaagcc
     1321 ggtgacggcg gaccagcgcg gcaaggtcga ggatctcgtc gcccggctgg ccaagaagca
     1381 gccggtggat ccgaggacgg tcaagctggt gagctcggtg tcgcagttcg ccgactgcga
     1441 cgcgctcatc ggcgacctcc gcaccgtcgg cgccgagcac gtcggcagcc gcggcttcgg
     1501 cgccaacacc ggtgaggtgg gcttcagcgc agagatgagg tcgatcagca agctggatgc
     1561 ggcggccgac agcatgaggc tgacgccgta cggcccggtc gcctccgtcg cgaacggcgg
     1621 cgagaccctc ggcaccaacg tgcaggtgac gggggtcgac gagctcgact cggtgaaggc
     1681 ggtcgggccg ctcatctacg acctcgacgg caaggggaac ctgcgcatca ccgacggagc
     1741 caccctgcag gtgcgctcga ccctcgacgt gacgccgaag gccatcggcg caccggacaa
     1801 gccgaagaag gacgatcccc agccaccgcc gacgagcgtc agccgattgc tcgtcgccgg
     1861 tgagcgcgtg gtgatcttcg gatccgagag cgagatctcc gagccggtga agggcgaccc
     1921 gtccgccacc cgagcggaga ccagctacct cacggtcacc ttcgtcgacg ccaccgacat
     1981 cgccaagccc aaggtcaccg accgggtgcg catcgagggc ggcttggtct ccgcccggat
     2041 ggtcgacggc gaggtccggc tggtgacgac ctccaacatg gcggacctcg ggttcgtcat
     2101 gcccaccacc cccacctccg tcgccaaggc cctcgaacag aaccgccggt ccgtcgcctc
     2161 ctcgacggcg gccgactgga tccccgactg gcagcgggcc agcggcgagc cgcagccgct
     2221 ggttccgtgc gatcgggtct acgtgcccga cacgttcgcc ggcgtggcca tgacctcgat
     2281 ggtcaccttc ccggccggcg cctcgaggtt cgagccggcc ggcacctcga tcctggcccc
     2341 cggcagcacg ctctacgccg gcctcgacaa ggtcgccatc agctccgagg tctgggtgga
     2401 tccgatcgac caagagcgcc tggagttcga cgactggcgg acggccgtcc acgagttccg
     2461 cttcagcccc ggccacgcac cgggctacga gggctcgggc atcgtcgacg ggtccaccat
     2521 cggccagttc gccttcggcg agctcggcga ctcgatcgcc gtggtgacca gcaggggcac
     2581 gccgtggtcg caggacacga cgaagctcgg cgtggacctc accgtgctca cgcccggcga
     2641 gaagggccgg ctcgacgtgg cctcgaaggt caaggacctc tccgacggca agggcgaggt
     2701 caccgcggtg cgcttcgtca ccgaccgcgt cttggtgtcc accgggctct tcggccgcca
     2761 gatcatcgtg atcgacgtga ccgaccccac cgcgccacga cgagcaggag gcgtggtcgt
     2821 cccgggtgac gtgggctact tccacccgct ccccgacggc cgagccctgg tcgtcggcag
     2881 tcgcagcgac acggtcagca tcggcaagga caaggtgagc cgcagctggg tccaagccca
     2941 cctcctcgac gtgaccgatg ccgacgcgcc gaagctggtc gccacctggg aacggccgtg
     3001 gtcagcggac gatgtgggct ccgaccacca tgccttcacc tactggccga accggaagct
     3061 ggccatgtgg ggcatctccg acaccaactg gtcccagcgc gacccgaacc cgaaccacgc
     3121 cgtcgtcctc gacaccgacg gaggggtgca ggtcgcgctg ccggtggcga accagccgaa
     3181 cgaggtcgcc cctccgtgcc cgagcgtgcc ggtccccgac gacgcccaga gcatggtcgg
     3241 ccccgacacc atcgtcctgc gctgcgacga cggcgccacc acgtcggtcg actggccccg
     3301 ctacgagtgc tcaaaggtcg acaagcagct ggtcgcccgc ttcgtgccgg aggaccagca
     3361 cgagaaggcc ttcttcctct gcaatccggc acccctgccc cgcgtcaccc gggtcctcgt
     3421 ggtggcgggc cggccgatcc tcctcaccga ccagacgctc gaggccctcg accccaagac
     3481 cttcgcctcc acggccatcg cctaccaccc cagctccggc gccttcttct cctgacccga
     3541 accccacgtg gcgttcgggc atcaccaggg agcgcgcatc cagctgcggc gtgaccgcga
     3601 gccgctggcg gggtctaccc gaggatgtgg gtggcgtagg ccgcggagtc gagcaactcc
     3661 atggccagct ccgcctgggt ggtgcccgcc ttctggcgga cagtccagtc ggcggcttcg
     3721 gcggtggtcg gagctcggcc cagcaggcag atgtagtcca ccgccaggtc ggtgttctcg
     3781 acctgcttcc ggcggaactc cgatgactcg gagaagccca ccacgaccgt gccccgggtc
     3841 cgccgcttgg tgtcgagcgt cttcgtccag tacgtgaccc cagccgggtc ggcagctc
//
LOCUS       DATMFS010000052         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_187864, whole genome shotgun sequence.
ACCESSION   DATMFS010000052 DATMFS010000000
VERSION     DATMFS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187864"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..777
                     /locus_tag="VNQ33_00965"
     CDS             97..777
                     /locus_tag="VNQ33_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96695.1"
                     /translation="MTALLRDSSMVAERNLRIMVRSPSSVLGAVLFPLIFLFGFFGVL
                     GRSMDARGLDYAQYLPPVIVIQAMFFTAIASAFFLADDRARGMFDRYRSLPIARGAIL
                     GGRAAADTVRASASLAVVVGGGTVLGFRFEPGPIGAVGFAGLALLAALTLAAGCGIIG
                     LIAPSPEAATSMLFLPYLPLLMLSTGFVPVEGFPGWLQPFVAWQPISLTVDALRAIAQ
                     GGPTAVPC"
     gene            851..1663
                     /locus_tag="VNQ33_00970"
     CDS             851..1663
                     /locus_tag="VNQ33_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96696.1"
                     /translation="MSSTAAPAPSALGRPPAELPWRRSVAVLTGRNAKRLLRSPVTLV
                     QSVCFPTLLLLTLLAAFGRVIGGSVSDYAARMVPQLVVAAGAFGAAGTGIAVHTDRVS
                     GMIDRLRALPIDRRGYLAAAVVADALRAAAAAAVLVVIGHAFGFRFHQGVAAMIGFFG
                     IAAAFSTIWAWLAVRVGLVASASEAVGSAMNGPVLMLFFLSSGFVPVAGFPGWLQPVV
                     RANPLSVAVEALIGLSAGGPVLVPVLQTAAWITALTLLLAPSAVRRYRSLAP"
     gene            complement(1685..>2542)
                     /locus_tag="VNQ33_00975"
     CDS             complement(1685..>2542)
                     /locus_tag="VNQ33_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWJ96697.1"
                     /translation="GDSTIEVEAAFYPLQWIAEQVGGSGVTVSSLTPPGAEPHDLELT
                     PKQVAGIGEADVVVKLSGFQPAVDDATGDRKNVFDVADVVDLDLTTTEHEHEGSEDHA
                     DEEEGHDDEKVADPHFWLDPTLLAEVAPALADRFGKLDPDHAAAYQQRADDTVALLDG
                     IDDDFAKGLDSCQSTDLVTSHQAFGYLARRYGLTQVGINGLSAEQEPSPGRLAEVTDF
                     VEDHGVRTIYFETLIPSDIARTIADETGADTAVLDPIEGLTDESAGDDYPSVMAANLE
                     ALRAGQSCS"
BASE COUNT          256 a          923 c          907 g          456 t
ORIGIN      
        1 catcgacgtc gtcgacatcg ggctgcgcac gccgacgctc gacgacgtgt tcctgaccct
       61 caccgggggt ccgggcgagc ccgctccgga ggagccgtga cggccctgct gcgcgacagc
      121 tcgatggtgg cggagcgcaa cctccggatc atggtccgca gcccgtcgtc ggtcctgggc
      181 gcggtcttgt tcccgctgat cttcctgttc gggttcttcg gtgtgctcgg acggtcgatg
      241 gacgctcggg ggctggacta cgcccagtac ctgccgccgg tgatcgtgat ccaggccatg
      301 ttcttcacgg cgatcgcctc ggcgttcttc ctcgctgacg accgtgctcg ggggatgttc
      361 gaccggtacc gctccctgcc gatcgcgcgc ggcgccatcc tcggaggccg cgccgcggcc
      421 gacacggtcc gcgcgtcggc atcgctggcc gtcgtggtcg gcgggggcac ggtcctcgga
      481 ttccgcttcg agccgggccc gatcggcgcc gtcgggttcg ccggcctcgc tctgctcgcc
      541 gcgctgacgc tcgccgccgg gtgcgggatc atcggcctga tcgcaccgag tcccgaggcg
      601 gcgacctcga tgctgttcct gccctacctg ccgctcctca tgctctcgac cggcttcgtc
      661 ccggtcgaag gcttccccgg ctggctgcag cccttcgtgg cctggcagcc gatcagcctg
      721 accgtcgacg cgctgcgcgc catcgcccaa ggcggtccga cggcggtccc gtgctgaagg
      781 cgaccagcct cttggcgacg ctcctcgtcg tgttcgtcgt gctcggtgcc cggctcaccc
      841 ggagggcgtc atgagctcga ccgctgctcc cgcgccctcc gcgctcggac gcccgccggc
      901 cgagctgccg tggcggaggt cggtcgccgt cctcaccggt cgcaacgcca agcggctgct
      961 gcggtccccg gtcaccctcg tgcagtcggt ctgcttcccg acgctgctgc tcctcacgct
     1021 gctggctgcg ttcggacggg tgatcggcgg ttcggtgagc gactacgccg cccgcatggt
     1081 gccccagctg gtggtcgcgg ccggcgcgtt cggtgcggcc gggaccggca tcgccgtcca
     1141 caccgaccgg gtgtcgggca tgatcgaccg cctgcgggcc ctgcccatcg accgccgggg
     1201 gtacctggcg gccgcggtcg tggccgatgc gctccgagcg gcggcggccg ccgcggtcct
     1261 ggtggtcatc gggcacgcgt tcggcttccg gttccaccag ggggtcgcgg cgatgatcgg
     1321 cttcttcggg atcgcggccg cgttctccac catctgggcc tggctcgcgg tccgggtcgg
     1381 cctcgtcgct tcggcgtccg aggccgtcgg ctcggccatg aacggacctg tcctgatgct
     1441 gttcttcctc tcgagcggct tcgtccccgt cgctgggttc cccggctggc tgcagccggt
     1501 ggtgcgcgcc aacccgctct cggtcgcggt cgaggcgctg atcgggctgt ccgccggtgg
     1561 cccggtcctg gtgcctgtgc tccagaccgc cgcgtggatc acggcgctca ccttgctcct
     1621 cgcgccatcg gccgtccgcc gctaccggtc gctcgcgccc tgacgggttc accgcccggc
     1681 tggctcacga gcaggactgg ccggcccgga gcgcctcgag gttcgccgcc atcaccgagg
     1741 ggtagtcgtc gcccgccgac tcgtcggtga ggccctcgat gggatcgagc acggcggtgt
     1801 cggccccggt ctcgtccgcg atggtccggg cgatgtcaga ggggatgagc gtctcgaagt
     1861 agatcgtccg gacgccgtgg tcctccacga agtcggtcac ctcggcgagc cgcccggggc
     1921 tcggctcctg ctcggcgctg aggccgttga tcccgacctg ggtcagtccg tagcggcggg
     1981 cgaggtaccc gaacgcctgg tggctggtca ccaggtccgt cgactggcac gaatcgaggc
     2041 ccttggcgaa gtcgtcgtcg atcccgtcca ggagggcgac ggtgtcgtcg gcgcgctgct
     2101 ggtacgccgc ggcgtggtcc gggtcgagct tgccgaaccg atcggccagg gccggtgcca
     2161 cctcggcgag cagcgtcgga tccagccaga agtggggatc ggcgaccttc tcgtcgtcgt
     2221 gaccctcctc ctcgtctgcg tggtcctcgc tgccttcgtg ctcgtgctcg gtggtggtga
     2281 ggtccaagtc gacgacatcc gccacgtcga acacgttctt gcggtcgccg gtggcgtcgt
     2341 cgacggcggg ctggaacccg gagagcttca cgacgacgtc ggcctcgccg atgccggcga
     2401 cctgcttcgg cgtgagctcc aggtcgtggg gctcggcccc cgggggagtg agggaggaga
     2461 cggtgacccc gctcccgccg acctgctcgg cgatccactg gagcgggtag aaggcggcct
     2521 ccacctcgat ggtgctgtcg cc
//
LOCUS       DATMFS010000053         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_94992, whole genome shotgun sequence.
ACCESSION   DATMFS010000053 DATMFS010000000
VERSION     DATMFS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94992"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VNQ33_00980"
     CDS             <1..453
                     /locus_tag="VNQ33_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96698.1"
                     /translation="VYDDVDFDKIVRPAARPAAKGIASRCLTEPGAYLTLVEGESLRA
                     SIFAEDPSDVPALVQHMDENSAKGVIPLPLFIGQGADDDVVDPKVTETYVDQLCDAGQ
                     DLDFYLLPGITHFTIVEKGSPVEEPLLRWTADRFAGEPVAKGCQRHDR"
     gene            509..1879
                     /locus_tag="VNQ33_00985"
     CDS             509..1879
                     /locus_tag="VNQ33_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ96699.1"
                     /translation="MSTDVSATIDRLRATFDSGRTADLGWRKRQLQGVIDLVRDNEEQ
                     LSAALAADLGKPAFDTWLTELNLVKDEAAHAIKHLGGWAKRTKRSVPLVAQPAKAWTE
                     PQPLGVVLIIAPWNYPLQLLLAPAVAALAAGNAMVLKPSELAPATSEAVAELVPKYLD
                     TDAVTVVEGGVPETTALLAEAWDHILYTGSTRVGQVVMEAAAKHLTPVTLELGGKSPT
                     IIAADANLPVAARRVAWAKILNAGQTCIAPDYLLVERSVSDEFAGLVVDELTTFRDKA
                     PATSIVNEHHLDRLEGLLDGHGGEELMPRKTDRGSRVMAPVVVRRPDPEAPVMQEEIF
                     GPILPFIEVDSVDEAISFIKARPRPLALYLFTSSGSTERAVLDQTHAGSVCINHLVYQ
                     AAVTDLPFGGIGPSGMGAYHGKSGFDTFSHQKSVLKRPTWTDPGFAYPPYTNQVQKIL
                     KFLTRW"
     gene            complement(2000..>2573)
                     /locus_tag="VNQ33_00990"
     CDS             complement(2000..>2573)
                     /locus_tag="VNQ33_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWJ96700.1"
                     /translation="DLLHQVEDAEGPKALVTTGTGRFFSNGLDLDYLGEHPSDLTSYL
                     FRVHSLFTRFLSLPCATVAAQNGHTFAAGAMLAICHDHRVMREDRGYWCLPEVDLGMP
                     FTEGMNALIPAMIPPRTAHEAMVTGRRYTGGDALAGGIVHELAAEDRVLPRAVEVAQS
                     FAGKAGPGIAGIRTHLHAHVIGHLHGTERG"
BASE COUNT          392 a          924 c          889 g          368 t
ORIGIN      
        1 gtgtacgacg acgtcgactt cgacaagatc gtgcgcccgg ccgcccgccc ggccgccaag
       61 ggcatcgcca gccgctgcct gaccgagccc ggcgcctacc tgacgctggt cgagggcgag
      121 tcgctgcggg cgtcgatctt cgccgaggac ccatcggacg tcccggcgct ggtccagcac
      181 atggatgaga actcggcgaa gggcgtgatc ccgctcccgc tcttcatcgg ccagggcgcc
      241 gacgacgacg tggtggaccc gaaggtgacc gagacctacg tcgaccagct gtgcgacgct
      301 ggtcaggacc tggacttcta cctgctgccg ggcatcaccc acttcacgat cgtggagaag
      361 ggctcaccgg tcgaagagcc gctcctgcgg tggacggccg atcgcttcgc cggcgagccg
      421 gtggcgaagg gatgccagcg ccacgaccgc tgaggcggtg tgaccgttgc cctgttcctc
      481 cgggacgggc gccggtagcg tttcggatat gagcaccgac gtctccgcca cgatcgaccg
      541 cttgcgggcc acgttcgact cgggtcgcac ggccgacctc ggctggcgga agcgccagct
      601 tcaaggcgtg atcgacctcg tgcgggacaa cgaagagcag ctgtcggctg ccctcgcggc
      661 cgacctcggc aagccggcgt tcgacacgtg gctcaccgag ctcaacctgg tgaaggacga
      721 ggcggcccac gccatcaagc acctcggcgg ctgggccaag cggacgaagc ggtcggtgcc
      781 gctcgtcgcc cagccggcca aggcctggac cgagccgcaa cccctcggcg tcgtgttgat
      841 catcgccccg tggaactacc cgctgcagct gctgctggcc ccggccgtcg ccgccctcgc
      901 cgccggcaac gccatggtgc tcaagccgtc ggagctggcc ccggccacgt cggaggcggt
      961 cgccgagctg gtgccgaagt acctcgacac cgacgccgtc acggtcgtcg aaggcggcgt
     1021 gcccgagacc acggccctgc tcgccgaggc gtgggaccac atcctctaca ccggctccac
     1081 ccgtgtcggg caggtcgtga tggaggcggc ggccaagcac ctcacgccgg tcacgctcga
     1141 gctgggcggc aagagcccga cgatcatcgc cgccgacgcc aacctcccgg tcgccgcccg
     1201 ccgcgtcgcc tgggcgaaga tcctcaacgc cggccagacg tgcatcgctc cggactacct
     1261 cctggtcgag cggtcggtca gcgacgagtt cgccggcctg gtggtcgacg agctcaccac
     1321 gttccgcgac aaggcgccgg ccacgtccat cgtgaacgag caccacctcg atcggctcga
     1381 gggcctgctc gacggccacg gcggtgagga gctgatgccc cgcaagaccg accggggctc
     1441 ccgcgtgatg gcgcccgtcg tcgtgcgccg tcccgacccc gaggcgccgg tcatgcagga
     1501 ggagatcttc ggcccgatcc tcccgttcat cgaggtcgac tccgtcgacg aggccatctc
     1561 gttcatcaag gcccgacccc gccccctggc cctctacctg ttcacgtcgt cgggctccac
     1621 cgagcgggcg gtgctggacc agacccacgc cgggtcggtg tgcatcaacc acctcgtgta
     1681 ccaggccgcg gtgaccgacc tgcccttcgg cggcatcggc cccagcggca tgggggcgta
     1741 ccacggcaag tccggcttcg acacgttcag ccaccagaag tccgtgctca agcgcccgac
     1801 ttggaccgac cccggcttcg cctacccgcc ctacaccaac caggtccaga agatcctcaa
     1861 gttcctcacc cgctggtagc cgcgggcgcc gcccacaccc gcaccccgca ccgaacgttc
     1921 gacgcccgtg gttgcacagc aacaggttgc acagcaaccg ttcgcatcga atggtcgggg
     1981 gagacggggc agtcgggggt cagccgcgct cggtgccatg gaggtggccg atgacgtggg
     2041 cgtggaggtg ggtgcggatg ccggcgatgc cggggccggc cttgccggcg aaggactggg
     2101 ccacctcgac ggcgcggggg aggacccggt cttcggcggc cagctcgtgg acgatgccgc
     2161 cggcgagtgc gtcgccgccg gtgtagcgcc ggccggtgac catcgcctcg tgtgccgtcc
     2221 tcggcgggat catcgccggg atgagcgcgt tcatcccctc ggtgaacggc atgcccaggt
     2281 cgacctcggg caggcaccag tagccgcggt cctcgcgcat cacccggtgg tcgtggcaga
     2341 tggcgagcat ggccccggcg gcgaaggtgt gcccgttctg ggcggcgacg gtggcgcacg
     2401 gcagcgacag gaaccgggtg aagaggctgt ggacccggaa caggtacgac gtcaggtctg
     2461 aggggtgctc gccgaggtag tcgagatcca ggccgttcga gaagaacctg ccggtcccgg
     2521 tggtgaccag cgccttgggg ccctcggcat cctccacctg gtggaggagg tcg
//
LOCUS       DATMFS010000054         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_2464, whole genome shotgun sequence.
ACCESSION   DATMFS010000054 DATMFS010000000
VERSION     DATMFS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VNQ33_00995"
     CDS             <1..790
                     /locus_tag="VNQ33_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWJ96701.1"
                     /translation="WFEDVRPEYVFLSAGTVGGVLANSTHPARFIYDNLLIHATVVHA
                     AHQIGVRKLLYLGSSCIYPRLAQQPMREEELLTGALEPTNESYAIAKIAGIKLCQAYR
                     TEYGDDFISAMPTNLYGTNDNFDLTSSHLLPALIRKFHEAKEAGAPSAEVWGTGTPQR
                     ELLHVDDLADACVFLMERYSDSLHINVGTGEDLSILQIAELTRDIVYPECEIVLDPSK
                     PDGMPRKLLDVTRLHDLGWKHRIGLEEGIRSTYEWYRDHAATLV"
     gene            857..1837
                     /gene="pseB"
                     /locus_tag="VNQ33_01000"
     CDS             857..1837
                     /gene="pseB"
                     /locus_tag="VNQ33_01000"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HWJ96702.1"
                     /translation="MDLAGKTILLTGGTGSFGHAFVDRVVEAHPGATVRVFSRDELKQ
                     SEMQARYSNHPQLRFFVGDIRNRSRLTRAMKGADIVVHAAAMKQVPACEYNPFEAVQT
                     NVLGAQHIVDAAIDNGVKQVVALSTDKAVNPVNIYGATKLCEEKIIIQGNAYAARSET
                     SLCCVRYGNVVGSRGSVVPLFRRQLAAGTPLTITDERMTRFWITLPEAVDLVMTALER
                     SVGGEIFIPKIPSMRVTELAEAMAPGKECDVVGIRPGEKLHEVLITDDESRHSIDAGD
                     IYVVLPEHPWWTDAPGWLDGAPLPDGFVYASGTNDVWLGADELRAQLETG"
     gene            1841..2926
                     /locus_tag="VNQ33_01005"
     CDS             1841..2926
                     /locus_tag="VNQ33_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ96703.1"
                     /translation="MIRYGSQQIDEDDIAAVVEVLRGDWLTQGPAVERFEQDLAARCE
                     ARHAVAFTSGTAALHAAAAAAGLGPGDRVATSPLTFSASAACAKYVGATPTLVDIDPA
                     TLNLRVDAVPDGLDALVAVHYAGLPIDLSGLAHRPRVVIEDAAHALGARTPDGPVGNC
                     ARSDMACFSFHPVKPITTGEGGAVTTNDGALAEALRRFRNHGTVPTPAQGGWAYEVRS
                     LGLNYRLPDIQAALGSSQLRKLEGWIIRRNDLASRYRSLLSELPVVLPPAAPEGHLHG
                     YHLFAVQVPERRRIFDGLRAAGIGVQVHYVPIHHHPLYADLGFEPDDLPAVERVYAGL
                     LSLPLHPGLSDADQDRVVAELARLLVP"
     gene            2923..>3183
                     /locus_tag="VNQ33_01010"
     CDS             2923..>3183
                     /locus_tag="VNQ33_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96704.1"
                     /translation="MTVAEPAPGGPVAAVVQARMGSTRLPGKVLAPLAGTTVLGLLLD
                     RLARSVSVDAMVVATTTGADDDPIVTEAERHGAVVVRGPVDDV"
BASE COUNT          496 a         1204 c         1029 g          454 t
ORIGIN      
        1 gtggttcgag gacgtgcgcc cggagtacgt cttcctctcc gccggaaccg tgggcggcgt
       61 gctggccaac agcacccacc ccgcccggtt catctacgac aacctgctca tccacgccac
      121 cgtcgtccac gccgcccacc agatcggcgt gcgcaagctg ctgtacctgg gcagctcgtg
      181 catctacccc cgcctggccc agcagccgat gcgcgaggag gagctgctga ccggtgcgct
      241 cgagcccacc aacgagtcct acgccatcgc caagatcgcc ggcatcaagc tctgccaggc
      301 gtaccgcacc gagtacggcg acgacttcat ctcggcgatg cccaccaacc tctacggcac
      361 caacgacaac ttcgacctca cgtcgagcca cctgctcccg gctctgatcc gcaagttcca
      421 cgaggccaag gaggccggag ccccctcggc cgaggtctgg ggcacgggca cgccccagcg
      481 ggagctgctc cacgtcgacg acctggccga tgcctgcgtg ttcttgatgg agcgctactc
      541 ggattcgctg cacatcaacg tgggcacggg cgaggacctg agcatcctcc agatcgccga
      601 gctcacccgc gacatcgtct accccgagtg cgagatcgtg ctggacccgt cgaagcccga
      661 cggcatgccg cgcaagctcc tcgacgtcac ccgcctccac gacctcggct ggaagcaccg
      721 catcggcctc gaagagggca tccgctccac ctacgagtgg taccgcgacc acgcggccac
      781 gctcgtgtga cggccaccct cggctgacca gctcaccatc acgcgatcgc cccaccgccg
      841 ctgccaagat gcccgaatgg acctcgcggg caagaccatc ctcctgaccg gcggaacggg
      901 ctcgttcggg cacgcgttcg tcgaccgggt ggtcgaggcg catccaggtg ccaccgtccg
      961 ggtcttctcc cgtgacgagc tgaagcagtc cgagatgcag gcgcgctact cgaaccaccc
     1021 gcagctccgc ttcttcgtcg gcgacatccg caaccggagc cgcctcaccc gggccatgaa
     1081 gggcgccgac atcgtggtgc acgccgccgc catgaagcag gtcccggcgt gcgagtacaa
     1141 ccccttcgaa gcggtgcaga ccaacgtctt gggtgcccag cacatcgtcg atgccgccat
     1201 cgacaacggg gtcaagcagg tggtcgcgct ctccaccgac aaggccgtca accccgtcaa
     1261 catctacggc gccaccaagc tctgcgaaga gaagatcatc atccagggaa acgcgtacgc
     1321 ggcacgctcg gagacctccc tgtgctgcgt ccgctacggc aacgtggtcg ggtcacgggg
     1381 ctcggtcgtc ccgctcttcc gaaggcagct cgcggcgggc acgccgctga ccatcaccga
     1441 cgagcggatg acgcggttct ggatcaccct gcccgaggcc gtcgacctgg tgatgacggc
     1501 gctcgagcgc agcgtcggcg gcgagatctt catcccgaag atcccctcga tgcgcgtgac
     1561 cgaactggcc gaggccatgg cacccggcaa ggagtgcgac gtggtcggca tccggccggg
     1621 cgagaagctc cacgaggtcc tgatcaccga cgacgagagc cgccacagca tcgacgcggg
     1681 cgacatctac gtcgtcctcc ccgagcaccc gtggtggacc gatgcacccg gctggctcga
     1741 cggcgccccg ctgccggacg gcttcgtgta cgcgagtggc accaacgacg tgtggctggg
     1801 ggccgacgag ctccgcgccc agctcgagac aggctgaggc gtgatccgct acggctccca
     1861 gcagatcgac gaggatgaca tcgccgcggt cgtcgaggtg ctgcgggggg actggctgac
     1921 gcagggcccg gccgtcgagc ggttcgaaca ggatctcgca gcacggtgcg aggcgcgcca
     1981 cgccgtcgcg ttcaccagtg gcaccgccgc gctccatgcc gcggccgccg cggccgggct
     2041 cggcccgggc gaccgggtcg cgacctctcc gctcaccttc tcggcgagcg cggcgtgcgc
     2101 gaagtacgtc ggcgcgaccc ccacgttggt cgacatcgat cccgcgacgc tgaacctccg
     2161 ggtggacgcg gtgcccgacg gtctcgacgc gctcgtcgcc gtgcactacg ccggcctgcc
     2221 gatcgacctg tcagggctgg cgcaccggcc gcgggtggtc atcgaagacg ccgcccacgc
     2281 cctcggcgcc cgcacacccg acggtcccgt cggcaactgc gcccgatccg acatggcctg
     2341 cttctcgttc cacccggtca aaccgatcac cacgggcgag ggcggggccg tcacgacgaa
     2401 cgacggcgcc ttggccgagg cgctccggcg gttccgcaac cacgggacgg ttccgacccc
     2461 agcgcaggga ggctgggcct acgaggtccg ctccttgggc ctgaactacc gactgcccga
     2521 catccaggcc gcgctcggga gcagccagct ccgcaagctg gaggggtgga tcatccggcg
     2581 caacgacctc gccagccggt accgctccct gctctcggag ctgccggtcg tccttccgcc
     2641 ggcggcgccc gaggggcacc tccacggcta ccacctcttc gccgtgcagg tgcccgagcg
     2701 ccgccggatc ttcgatggcc tgcgagccgc gggcatcggg gtccaggtcc actacgtgcc
     2761 gatccaccac cacccgctct acgccgatct ggggttcgag cccgacgacc ttcccgcggt
     2821 cgagcgggtc tacgcagggc tgctctccct tccgctccac cccgggctga gcgacgccga
     2881 tcaggaccgc gtcgtcgccg agctcgcccg gctcctcgtg ccgtgaccgt cgctgagccc
     2941 gctcctggtg ggccggtggc agcggtggtg caggcgcgga tgggcagcac gaggcttcct
     3001 ggcaaggtcc tggcacccct tgcgggcacc accgtgctgg ggctgctgct cgaccgcctc
     3061 gcccggtccg tgtccgtcga cgcgatggtc gtggcgacga ccacgggggc cgatgatgac
     3121 ccgatcgtca ccgaggccga gcgccacggc gcggtcgtcg tccgcggccc ggtcgacgac
     3181 gtc
//
LOCUS       DATMFS010000055         3082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_25385, whole genome shotgun sequence.
ACCESSION   DATMFS010000055 DATMFS010000000
VERSION     DATMFS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25385"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VNQ33_01015"
     CDS             complement(<1..333)
                     /locus_tag="VNQ33_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ96705.1"
                     /translation="MPRLHLVRHGRSEAAWADHLDPGLHTDGRAQAAAVAAELAGRIE
                     PVPIWSSPLLRTQQTAAPLAATWSSTIVLAPAFGEIPSPSTDPGARRAWLASAMASRW
                     SDLGPVIDG"
     gene            complement(341..1201)
                     /locus_tag="VNQ33_01020"
     CDS             complement(341..1201)
                     /locus_tag="VNQ33_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWJ96706.1"
                     /translation="MGTIVSFHAHPDDESIASGGTLALAADAGHRVVLVFGTRGELGE
                     PVPGVLAEGEQLALRRTAEVFRSAEVLGVDRVEFLGWMDSGMVGEPTNGVPGCFWQAD
                     VEHAARRLAVILEEEQPDVLTIYDDNGGYGHPDHIQVHRVGQRAAELVDVPVVAQGTM
                     NRDLIRRAWAMAAEMGIEVPNPTEEEQPPEGLTAEEGVAAAVTFGKAESELTHFVDAS
                     TVTDRKRASMLSHASQIGPDHFMASMPPEIFAYVFGQEWYIVEHRHDGALPPLAAELF
                     QPKPEPEPVP"
     gene            1363..2022
                     /locus_tag="VNQ33_01025"
     CDS             1363..2022
                     /locus_tag="VNQ33_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWJ96707.1"
                     /translation="MTDHDDQDSDARAQTRRKFLAGAGLAAGAAGAATLLGASPASAS
                     TPATGEFDLDLKWADITGVPWVLDDVDLGDGGFAKGVFSAFGDMAALSIRILVGEDAD
                     PGDGPWVLEASSMPSGYTPATPPNDLSPTGTPGWGGVGNVVDFTKMSDNNHELLVSWN
                     NFTSIGNGTLLTWTIPNVHNDASGSVLFNYGGSAPYPSPLGASSQVVGILVYLREPEE
                     P"
     gene            complement(2055..>3082)
                     /locus_tag="VNQ33_01030"
     CDS             complement(2055..>3082)
                     /locus_tag="VNQ33_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWJ96708.1"
                     /translation="AFGQGAGAEPPADILARGRRSVAVDLKNPEGVATVLDLVAEADV
                     LIEGFRPGVMERLGLGPDVCLERNPRLVYGRMTGWGQQGPYASAAGHDINYIALAGAL
                     EPLGRAGEQPTPPLNLVGDFGGGGMMLAFGVCAALVEVARSGEGQVIDAAMVDGAASL
                     MTMTWSFKHMGIWADERGTNMLDTGAHFYDTYEAADGKYVSIGSIEPQFYAELLRLTG
                     IDPESLPKQMDKSQWPALKERFAEVFKTKTRDEWCELMEHTDVCFAPVLSMDEAPEHP
                     HIAERETFTTVAGLVQPAPAPRFSRTPGSIERPPPHAGQHTDEALEAWGISADRIAQL
                     REAKAIV"
BASE COUNT          433 a         1080 c         1136 g          433 t
ORIGIN      
        1 cccgtcgatc accggaccga ggtccgacca gcgcgacgcc atcgccgagg ccagccaggc
       61 gcgccgcgct cccgggtcgg tggacggcga cgggatctcc ccgaaggcgg gggcgaggac
      121 gatggtgctg gaccacgtcg ccgcgagggg tgccgcggtc tgctgggtcc gcaggagggg
      181 cgacgaccag atcgggaccg gttcgatccg accggcgagc tcggcggcga cggcggcagc
      241 ctgggctcgg ccgtccgtgt ggagacccgg gtcgaggtgg tcggcccagg ccgcctcgga
      301 gcggccgtgg cggacgaggt gcaggcgggg cacagccggc ctacggcacc ggctcgggct
      361 cgggcttcgg ctggaacagc tcggcggcga gcggcggcag ggcgccgtcg tggcggtgtt
      421 cgacgatgta ccactcctgc ccgaacacgt aggcgaagat ctccggcggc atcgacgcca
      481 tgaagtggtc cgggccgatc tggctggcgt gcgacagcat cgatgcccgc ttgcggtcgg
      541 tgaccgtcga cgcgtcgacg aagtgggtca gctcgctctc ggccttgccg aaggtgacgg
      601 cggcggcgac gccctcctcg gcggtgaggc cctccggcgg ctgctcctcc tcggtggggt
      661 tcgggacctc gatgcccatc tcggccgcca tggcccacgc ccggcggatg aggtcgcggt
      721 tcatcgtgcc ctgggccacg accggcacgt cgacgagctc ggctgcccgc tgccccaccc
      781 ggtggacctg gatgtggtcc gggtggccgt acccgccgtt gtcgtcgtag atggtgagga
      841 catcgggctg ctcctcctcc aagatgaccg cgagccgccg ggcggcgtgc tcgacgtcgg
      901 cctgccagaa gcagccgggc acaccgttgg tgggctcgcc gaccatcccg gagtccatcc
      961 accccaggaa ctcgacccgg tcgacgccga ggacctcggc cgaccggaac acctcagccg
     1021 tgcggcgcag ggccagctgc tcgccttcgg ccaggacgcc cggaaccggt tcgccgagct
     1081 cgccgcgggt gccgaacacc aagacgacgc ggtgaccggc atcggccgcc agggcgagcg
     1141 tgccgcccga cgcgatcgac tcgtcgtcgg ggtgggcgtg gaagctgacg atggttccca
     1201 tgcgccggaa cgctaccggc agcggatgac gcccgaacca cgtccaggag gtgacgctcc
     1261 ggtgggcatc cccagatccg atgcaggtga tccgtgggag cgatatggtg cccgcaggcg
     1321 gatcgacggg acccgccacg gcaaagagaa ggcggtttcg gcatgaccga tcacgatgac
     1381 caagactccg acgcgcgcgc ccagacgcgg cggaagttcc tcgcgggcgc aggcctcgcg
     1441 gccggtgccg cgggcgcagc gaccctcctc ggggcctcac cggcctcggc cagcacgccg
     1501 gcgaccggcg agttcgacct cgacctcaag tgggcggaca tcacaggtgt cccgtgggtc
     1561 ctcgacgacg tcgacctcgg cgacggcggg ttcgccaagg gggtcttctc cgccttcggc
     1621 gacatggccg cgctgtcgat ccgcatcctc gtcggcgagg acgcggaccc cggtgacgga
     1681 ccctgggtgc tcgaggcctc gtccatgccg agcgggtaca cccccgccac gccaccgaac
     1741 gacctctcgc cgaccgggac cccaggctgg ggcggcgtgg gcaacgtcgt ggacttcacc
     1801 aagatgtcgg acaacaacca cgagctcctg gtgagctgga acaacttcac cagcatcggc
     1861 aacggcacct tgttgacgtg gaccatcccc aacgtccaca acgacgcatc aggcagcgtg
     1921 ctcttcaact acgggggctc ggccccgtac ccgagccccc tcggcgccag ctcccaggta
     1981 gtcgggatcc tcgtctacct ccgggagccc gaggagccct gacctcgacg ggtggcgccc
     2041 gcccggctcc gcgatcagac gatggccttg gcctcgcgga gctgggcgat gcggtccgcg
     2101 ctgatgcccc aggcctcgag ggcctcgtcg gtgtgctggc cggcgtgggg cggcgggcgc
     2161 tcgatgctgc ccggcgtgcg gctgaagcgc ggcgccggag ccggctggac cagcccggcg
     2221 acggtcgtga acgtctcccg ctcggcgatg tgggggtgct cgggcgcctc gtccatcgac
     2281 agcaccggtg cgaagcacac gtcggtgtgc tccatcagct cgcaccactc gtcgcgggtc
     2341 ttggtcttga agacctcggc gaagcgctcc ttgagcgccg gccactggct cttgtccatc
     2401 tgcttgggca gggactcggg gtcgatgccg gtgaggcgga gcagctcggc gtagaactgc
     2461 ggctcgatcg agccgatgga cacgtacttc ccgtcggccg cctcgtacgt gtcgtagaag
     2521 tgggcgccgg tgtcgagcat gttggtgccc cgttcgtcgg cccagatgcc catgtgcttg
     2581 aaggaccacg tcatcgtcat gaggctggcg gcgccgtcga ccatggccgc atcgatcacc
     2641 tggccctcgc cggagcgggc gacctccacc agggcggcgc acacgccgaa ggcgagcatc
     2701 atcccgccgc cgccgaagtc gcccaccagg ttgagcggcg gggtgggctg ctcgccggcc
     2761 cggccgagcg gctcgagcgc accggcgagg gcgatgtagt tgatgtcgtg gccggccgcc
     2821 gaggcgtagg gcccttgctg tccccacccg gtcatgcgcc cgtagacgag ccgggggttg
     2881 cgctcgaggc acacgtcggg gccgaggccg aggcgctcca tgacgccagg gcggaagccc
     2941 tcgatgagga cgtcggcctc ggccacgagg tcgagcacgg tggcgacgcc ctcggggttc
     3001 ttgaggtcca cggcgacgga ccggcggccg cgggcgagga tgtcggcggg tggctcggct
     3061 cccgcgccct ggccgaaggc gt
//
LOCUS       DATMFS010000056         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_48505, whole genome shotgun sequence.
ACCESSION   DATMFS010000056 DATMFS010000000
VERSION     DATMFS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48505"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VNQ33_01035"
     CDS             <1..237
                     /locus_tag="VNQ33_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96709.1"
                     /translation="ERAEAAANRSASAWGERCNEGGLDCSFVIDDCSEGGGECMVIEL
                     QYDLKNHPRVASAPLLDGLLPDSMSTRAVVEVSA"
     gene            251..1837
                     /locus_tag="VNQ33_01040"
     CDS             251..1837
                     /locus_tag="VNQ33_01040"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWJ96710.1"
                     /translation="MTISHRRSTVEAARRGESGAVIVLVALTISVLVTATAFTVDLGR
                     ISTERRDLQKTADVTALDLSRRLDGRSAAEIVADPAWEAALDASLARNRFDAGAGSVT
                     VAIGSWDPVTEVFTPSTGSQVPSAVRVELGSTVDYAFAPGSASPSRQAIAGQTASAGH
                     QVGSFATRLDTGRSALLGALVGDALGVTVAGYDGLAGAQISLPVLLEELDLSLGDVDQ
                     LLAADVTVAELLRAEAEVLRRSGDLVRADTLDGLVLALPNPDQIIHLEDLLAIIPGGE
                     AAAALADIDAQDLLNATALVANGTNLLDVPAADLTVGGQAVTVSTSVQVIEAPRWAFG
                     PVGTTAATSQATITLELQVTIPDVSDSRITVKLQAASGEATSTAIGCRDPKTLDLAVW
                     TGLLTTRADISTRLSLLGVPVADVVLHAAASAPPATTPVLFDLPPDAYGVPREAATPA
                     TSIGTASVVTDEVTLLGLPIAGGVAPLVDALTSTVLGPVLTQVETAVIQPLADALGLT
                     VAGVDVAPLAITCSGPELVG"
     gene            1885..3456
                     /locus_tag="VNQ33_01045"
     CDS             1885..3456
                     /locus_tag="VNQ33_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ96711.1"
                     /translation="MATDQELRGHKRHVVALSDIHLGTLEPTVWYQPHLHEPYLLHLL
                     DWVVDQAEHIRELVLLGDIVDFWTYPADVRPPAFAEIAACHPAVFDLDGALARALDAL
                     DGQVTYVPGNHDQGITADEVASIRSRAGHHVRLVEAVPYLPVPGLAMAHGHHDTLFNA
                     PTTTGPFAPLPLGYFVTRAVATRWAQGLGDGQTVAELAGQGAPNGIDLRSLAGVASGV
                     GARSVSATVLDFVASATAMNPLTGIVMPDGSTATLLDARAAYEHTWTEWADANGGGIA
                     GQSAAGRATLADFDGTYLGWFAQRMAFEHDCDLVVMGHTHVPIGGLDTSLVNYINTGF
                     DCPSEPDLTRDLSPQHVTFGVIDTRPADDDPDTAPFDPGIWAVSDTGDGLRCHPIEVP
                     TTSVVSGATMDYSCYVVVDNRFGTSDLELVEATASYGTFVVAPPERIASGAEGRFWLQ
                     DLLGAAGSDGRAAYRRTDDPGGDPIELRFTCPTVGTNACSGPGSFATRAGGNDWREER
                     VALWGHPFFVEFEIA"
     gene            3574..>3814
                     /locus_tag="VNQ33_01050"
     CDS             3574..>3814
                     /locus_tag="VNQ33_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96712.1"
                     /translation="MSRPKLPFTLPLDHLRITRWDDPVLDAVGHDPRSSYVERYWLAL
                     LGPSTTFLIRRITAELEAHPNGFDLPLEETAHAIGL"
BASE COUNT          497 a         1538 c         1253 g          526 t
ORIGIN      
        1 gagcgcgccg aggccgccgc caaccgctcg gcgagcgcgt ggggcgagcg ctgcaacgag
       61 ggcggcctcg actgctcgtt cgtgatcgac gactgctccg aaggcggcgg cgagtgcatg
      121 gtcatcgagc tccagtacga cctgaagaac cacccgcgcg tcgccagtgc gccgctgctc
      181 gacggcctgc tgcccgactc gatgtcgacg cgggccgtgg tcgaggtgtc ggcgtgaacc
      241 gggcccagcc gtgacgatca gccaccggcg cagcaccgtc gaggcggccc ggcggggcga
      301 gtccggcgcg gtgatcgtgc tggtggccct gaccatctcg gtgctcgtca ccgcgaccgc
      361 gttcacggtc gacctcggcc ggatctccac cgagcggcgc gacctccaga agacggccga
      421 cgtcaccgcc ctcgacctgt cgcgccgcct cgacggccgg agcgcggccg agatcgtggc
      481 cgatccggcg tgggaggccg cgctggacgc ctcgctcgcc cggaaccgct tcgacgccgg
      541 cgcggggagc gtcacggtcg ccatcggctc ctgggacccg gtcaccgagg tgttcacgcc
      601 gtccacggga tcccaggtcc cgagcgccgt ccgcgtcgag ctcggatcga cggtcgacta
      661 cgcgttcgct cccggcagcg cctcgccctc ccgccaggcc atcgccggcc agaccgcatc
      721 cgcgggccac caggtgggca gcttcgccac ccgcctcgac accgggcggt cggcgctgct
      781 cggcgccctc gtcggcgatg cgctcggggt gaccgtggcc ggctacgacg ggctggccgg
      841 cgcccagatc tccctgccgg tgctcctcga ggagctcgac ctctcgctcg gcgacgtgga
      901 ccagctcctg gcggccgacg tgaccgtggc cgagctgctc cgcgccgaag ccgaggtcct
      961 gcgccgctcg ggcgacctcg tgcgggccga caccctcgac ggcctggtcc tggccctgcc
     1021 caaccccgat cagatcatcc acctcgagga cctgctcgcc atcatcccgg gcggcgaggc
     1081 cgccgccgcc ctcgccgaca tcgacgccca ggacctcctg aacgccacgg ccctcgtcgc
     1141 caacggcacc aacctcctcg atgtcccggc tgccgacctc acggtgggcg gccaggccgt
     1201 gaccgtgtcc accagcgtcc aggtgatcga ggccccgcgg tgggcgttcg gtccggtcgg
     1261 gaccaccgcg gccacgtccc aggcgacgat caccctcgag ctgcaggtca cgatccccga
     1321 tgtgtccgac tcgcgcatca ccgtgaagct gcaggcggca tcgggcgagg ccacgtcgac
     1381 ggccatcggc tgccgcgacc ccaagacctt ggacctcgcg gtgtggacgg gcctcctcac
     1441 cacccgggcc gacatcagca cgcggctctc gctcctcggc gtgccggtgg ccgacgtcgt
     1501 cctccacgcc gccgcgtcgg cgccgccggc cacgacgccc gtcttgttcg atctgccgcc
     1561 cgatgcctac ggcgtgcccc gcgaggcggc caccccggcc acctcgatcg gcaccgcctc
     1621 ggtggtcacc gacgaggtca ccctcctggg gctgccgatc gccggcggcg tggccccgct
     1681 cgtcgacgcc ctcaccagca cggtgctcgg cccggtgctc acgcaggtgg agacggccgt
     1741 gatccagccg ctggccgatg cgctcggcct caccgtcgcc ggcgtcgacg tggcgccgct
     1801 ggcgatcacg tgctccgggc ccgagctcgt cggctgaccc gcccggcgct gcggcgccgt
     1861 tcccgccggt gcgatgatgg gatggtggcg accgaccagg agctccgcgg gcacaagcgc
     1921 cacgtcgtgg ccctgagcga catccacctc gggacgctcg aacccaccgt ctggtaccag
     1981 ccccacctgc acgagcccta cctgttgcac ctcctggatt gggtggtgga ccaagccgag
     2041 cacatccgcg agctggtcct gctgggtgac atcgtcgact tctggacgta tccggccgac
     2101 gtccgtccgc cggccttcgc cgagatcgcc gcgtgccacc cggccgtgtt cgacctcgac
     2161 ggcgccctcg cccgcgccct cgatgcgctc gacggccagg tcacctacgt gcccgggaac
     2221 cacgaccagg gcatcacggc cgacgaggtc gccagcatcc gcagccgcgc cggccaccac
     2281 gtccgcctcg tggaagcggt cccctacctc ccggtacccg gcctggcgat ggcccacggc
     2341 caccacgaca cgctcttcaa cgcccccacc accaccggcc ccttcgcccc gctcccgctg
     2401 ggctacttcg tcacccgcgc cgtcgccacc cgctgggccc agggcctcgg cgacggccag
     2461 acggtcgccg agctggccgg tcagggcgcg ccgaacggca tcgacctgcg ctcgctggcc
     2521 ggggtggcgt ccggggtggg ggctcgctcc gtgtcggcca cggtcctcga cttcgtcgcc
     2581 tcggccacgg cgatgaaccc gctcaccggc atcgtcatgc ccgacggatc caccgcgacc
     2641 ctgctcgacg cccgggcggc gtacgagcac acgtggaccg agtgggccga cgcgaacggc
     2701 ggcggcatcg ccggccagtc cgccgccggc cgcgccaccc tggccgactt cgacgggacc
     2761 tacctcgggt ggttcgccca gcgcatggcc ttcgagcacg actgcgacct ggtggtcatg
     2821 ggccacaccc acgtgccgat cggcgggctc gacacctcgc tcgtgaacta catcaacacc
     2881 gggttcgact gcccctccga gcccgacttg acccgcgacc tgtcgcccca gcacgtgacg
     2941 ttcggcgtga tcgacacccg acccgccgac gacgaccccg acacggctcc gttcgacccc
     3001 gggatctggg ccgtctccga cacgggcgac gggctgcgct gccaccccat cgaggtgccg
     3061 acgacctcgg tcgtgagcgg cgccacgatg gactactcct gctacgtggt ggtcgacaac
     3121 cggttcggga ccagcgacct ggagctggtg gaggccaccg cgtcgtacgg gaccttcgtg
     3181 gtcgcgccgc cggagcgcat cgcctccggc gccgaaggcc ggttctggct ccaggacctc
     3241 ctcggtgccg ccggctccga cggccgcgcc gcctaccggc gcaccgacga ccccggcggc
     3301 gatccgatcg agctgcggtt cacctgcccg accgtcggca ccaacgcctg ctccgggccc
     3361 ggctccttcg ccacccgagc cggcggcaac gactggcgcg aggagcgcgt ggccctctgg
     3421 ggccacccct tcttcgtcga gttcgagatc gcctgaccag cgcctgcgag ccggaccagc
     3481 gccacatcgg gagcatctgg agcgggcctc ttgcgagcgc ggcgatcaga catgaaagtg
     3541 catgaaacgc cgttcatgcc ctcggaggtc gccgtgtctc gtcccaagct cccgttcacc
     3601 ctgccgctcg accacctgcg gatcacccgc tgggacgatc cggtgctcga cgccgtcggc
     3661 cacgaccccc ggtcgtccta cgtcgagcgg tactggctgg ccctgctcgg gccctccacc
     3721 acgttcctca tccggcgcat caccgccgag ctcgaggcgc acccgaacgg cttcgacctg
     3781 ccgctcgagg agaccgccca cgccatcggc ctcg
//
LOCUS       DATMFS010000057         3251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_234454, whole genome shotgun sequence.
ACCESSION   DATMFS010000057 DATMFS010000000
VERSION     DATMFS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234454"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1781)
                     /locus_tag="VNQ33_01055"
     CDS             complement(213..1781)
                     /locus_tag="VNQ33_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ96713.1"
                     /translation="MTGNFDDAATLWELVEMRATATPDALLLIDESGRTLTCQEFRDR
                     AERAAAGFAALGVRAGTRVTWELPNRFETVIASFALARLGAVQNPILHFYRHKEVGYA
                     IRATSAEFVLVPGTWNGFDFAAMVDELTADLPDKPVVIDVFADLPDADPAGLADIPAP
                     TDGDEIRWIYFTSGTTSDPKGVRHTDRTLITGANGLAVALDMSGDDVGSIAFPFSHIA
                     GPDYFGTMLLAGFPAVLFEAFVPATAIGVLAANGVTMVGGGTAFYQMYLGEQRKDPAT
                     PIIPTLRKMSGGGAPMPPEIYEEIKREFGVKTCHGYGMTECPMICEGSPHDTDEQLAN
                     TVGKPVVGLEMRIVKDDGTVGGPDEDGEVRLKGPMVFGGYTDPAATAEAFDEDGWFRT
                     GDLGHIRPDGHVVLTGRLKDVIIRKGENISAAEIENLLYAHPKVGDVAVIGLADRERG
                     ERVCAVVERVADASPDDDLTFDEMVAYLGEHQLARFKTPEQLEVVDALPRNETLRKIL
                     KFKLREAYAAKPWP"
     gene            complement(1923..2819)
                     /locus_tag="VNQ33_01060"
     CDS             complement(1923..2819)
                     /locus_tag="VNQ33_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96714.1"
                     /translation="MADLLAAVDDLAQDIRDELDRTIAQSVDSEFSGAEHDGTGQHVP
                     QAEEEGGDQSPVGQEPTSIAEILATLRQIVEGLERPTALASVAYELQKVYGPDVTKSW
                     AGYGTFKSLLTAAAPDVAISLVGPGYVFPEGTTKALITTTPGAVPPGDQTLEEVDSKI
                     PPIVVRLRSVDRWVPCLPPDRLRELYTGIVLVLAHETWDELGFTNGSPGSIGLAQINE
                     LTRLARDRLAIEGITLNRHHLDYVLKALLWSGNLRAGLPPDQAVEIVRQWIYNRAVAM
                     GLSSDPPTDLDSLTDWLDAARA"
     gene            complement(2834..>3251)
                     /locus_tag="VNQ33_01065"
     CDS             complement(2834..>3251)
                     /locus_tag="VNQ33_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HWJ96715.1"
                     /translation="KDTQRAWKKLQKPQYRSLASDIMNVPEILDDLVGVRIVCNNLSD
                     IQRVKEIIGSLPEFDPDSLTGLAIEGESERFYLDAPKPSGYRAYHINLVTVVPGSDHV
                     HPMRAELQVRTLLQDGWGELTHEDTYKAVQSSRLLR"
BASE COUNT          516 a          987 c         1157 g          591 t
ORIGIN      
        1 agccgccgcg aggtgggcgc cggaccgcag cgtccgcttc ctcgccgaca cgtccgtcat
       61 ctccacctgg gccgacgcca agccctgagc tcccgccctg gcctgaactc ggtgtgcgaa
      121 acgtagagca tcagttcgca caccaacttc ggtgggtggg ccggtgggtg ggcagtgggt
      181 ggggcggtgg gtggggcggt gggggtcccc ggtcagggcc agggcttggc ggcgtaggcc
      241 tcgcggagct tgaacttgag gatcttccgc agggtctcgt tgcgggggag ggcgtcgacc
      301 acctcgagct gctcgggcgt cttgaagcgg gcgagctggt gctcaccgag gtaggccacc
      361 atctcgtcga aggtgaggtc gtcgtcgggc gaggcatcgg cgacgcgctc gacgaccgca
      421 cacacccgct cgccgcgctc gcggtcggcc aggccgatga ccgcgacgtc cccgaccttg
      481 gggtgggcgt agaggaggtt ctcgatctcg gcggccgaga tgttctcgcc cttgcggatg
      541 atgacgtctt tgagccggcc ggtcagcacg acgtggccgt cggggcggat gtggccgagg
      601 tcgccggtgc ggaaccagcc gtcctcgtcg aacgcctcag cggtggcggc ggggtcggtg
      661 tagccaccga agaccatcgg gcccttgagc cggacctcgc cgtcctcgtc ggggccgccg
      721 acggtcccgt cgtccttcac gatgcgcatc tccagcccga ccaccggctt gccgaccgtg
      781 ttggccagct gctcgtcggt gtcgtgcggg gagccctcgc agatcatcgg gcactcggtc
      841 atgccgtagc cgtggcaggt cttgacgccg aactcccgct tgatctcctc gtagatctcg
      901 ggcggcatcg gggcgccgcc gccggacatc ttgcgcagcg tcgggatgat cggggtggcc
      961 ggatccttgc gctgctcgcc caggtacatc tggtagaacg cggtgccgcc gccgaccatg
     1021 gtgacgccgt tggcggcgag caccccgatg gcggtggcgg gcacgaaggc ctcgaacagc
     1081 acggccggga acccggcgag gagcatggtg ccgaagtagt cgggcccggc gatgtggctg
     1141 aacgggaagg cgatcgagcc cacgtcgtcg ccgctcatgt cgagggcgac ggccaggccg
     1201 ttggcgccgg tgatgagggt gcggtcggtg tggcggacgc ccttcgggtc cgacgtggtg
     1261 ccggaggtga agtagatcca gcggatctcg tcgccgtcgg tgggcgccgg gatgtccgcc
     1321 aggccggccg ggtcggcgtc ggggaggtcg gcgaagacgt cgatgacgac gggcttgtcg
     1381 ggcaggtcgg cggtgagctc gtcgaccatg gcggcgaagt cgaagccgtt ccaggtgccg
     1441 ggcacgagga cgaactcggc gctggtcgcc cggatggcgt agccgacctc cttgtgccgg
     1501 tagaagtgca ggatcgggtt ctggacggcg cccaggcggg cgagggcgaa gctggcgatg
     1561 accgtctcga accggttcgg cagctcccac gtgacccggg tgccggcgcg cacgccgagg
     1621 gcggcgaagc cggcggcggc ccgctcggct cggtcgcgga actcctggca cgtgagcgtg
     1681 cggcccgact cgtcgatgag cagcagcgcg tcgggcgtgg ccgtcgcccg catctccacc
     1741 agctcccaca gcgtcgcggc gtcgtcgaag ttcccggtca cgttcgtccc ctggctcgtg
     1801 ctcgcccatc cggtatctga tggaccgtca gggaatctag accgcgggtg acacaagcga
     1861 agtggaaccc atcgcgtacg cgatgggttc acggtcgagt tcttcgaccg tgaacgctgt
     1921 gcttaggcgc gggctgcgtc cagccagtca gtgaggctgt cgagatcggt aggagggtcc
     1981 gagctgaggc ccatcgctac ggcacggttg tagatccact gacgcacgat ttcgacggct
     2041 tggtctggag gcaaccctgc tcggagattg ccgctccaca acagggcctt gaggacatag
     2101 tccagatgat gccgattgag cgtgatgccc tcaatggcga gacgatcgcg tgcaagtcgg
     2161 gtgagctcat tgatctgtgc tagcccgatc gatccgggcg acccgttggt gaagcccagc
     2221 tcatcccagg tctcgtgtgc tagcacaagc acgatccccg tgtagagctc acgcagcctg
     2281 tctggcggca gacaaggcac ccagcggtcg actgaacgga gacggactac gatgggtggg
     2341 atctttgagt cgacctcctc caaggtctgg tcgcctggtg gaacggcccc cggggtggtg
     2401 gtgatgagcg cctttgtggt tccctcagga aacacataac cagggccaac aagagagatc
     2461 gctacatctg gtgctgcagc cgtgagcaga gacttgaatg tgccgtatcc cgcccaagat
     2521 ttcgttacgt ctgggccata gaccttctga agttcgtagg ccactgatgc gagtgcggtg
     2581 gggcgttcga ggccttctac gatctgccgg agcgtggcaa ggatctcggc tatggatgtc
     2641 ggctcctgcc ccacggggga ttggtcccca ccttcctcct cggcttgcgg gacatgttgc
     2701 ccggtgccgt cgtgctcggc gcccgagaac tcagagtcga cagactgggc gatggttcgg
     2761 tcgagttcgt cgcgaatgtc ttgagccaga tcatcgacgg ccgcaaggag atcggccatc
     2821 cgtttcgcca aggtcatcgc aagaggcggg agctctgcac cgccttgtag gtgtcctcgt
     2881 gagtcagttc gccccatccg tcctgcaaaa gggttcgaac ctgaagctcg gcacgcattg
     2941 ggtgaacgtg atccgatccg ggaacaaccg tgacaaggtt gatgtggtat gcccggtatc
     3001 ccgacggctt aggtgcgtcg aggtagaatc gttcggactc tccctcaatg gcgagtccgg
     3061 tgagcgaatc cgggtcaaac tcaggaaggg acccgatgat ctccttcacg cgctgaatat
     3121 cggacaggtt gttgcatacg atgcggacgc cgacgagatc atcgaggatc tctggcacat
     3181 tcatgatatc tgaggcgagt gatcgatact gaggtttctg cagcttcttc caagcgcgct
     3241 gcgtgtcctt g
//
LOCUS       DATMFS010000058         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_164622, whole genome shotgun sequence.
ACCESSION   DATMFS010000058 DATMFS010000000
VERSION     DATMFS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164622"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VNQ33_01070"
     CDS             complement(<1..180)
                     /locus_tag="VNQ33_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96716.1"
                     /translation="MTTTEPTAPTTELDFVDADGHIVEPPFGLQEYAAAEFRDRVYHL
                     EIDGDGEEWVVIGDAR"
     gene            complement(255..1583)
                     /locus_tag="VNQ33_01075"
     CDS             complement(255..1583)
                     /locus_tag="VNQ33_01075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ96717.1"
                     /translation="MTLDDLILISVDDHVVEPPDMFEGRIPSAFADRAPKLVSKDDGS
                     DQWVYEGKDIPNVGLNAVAGRPPAEYGVEPTSFAEIREGAYDVDRRIDDMNANGVLAS
                     MNFPSFPQFCGQMFNNSEDKELGLVVLKAYNDWHLEDWCGAHPGRFIPLGLIPYWDPE
                     LMAAEVRRLASLGCHAVTWSENPEKLGHPSFHNPHWDPFWAACEETGTAVCIHIGSSS
                     QVAITSMEAPITTMLNLTAIGIFQTAADLLWSPVLRRFPKVQFVLSEGGTGWIPYFLE
                     RADYVYRHHKVWTGVDFGGQLPSDVFRERFATCFIDDTHGLENRHQVGLSNMCWEADY
                     PHSDSTWPTSPEQLWTSLQATGVPDDEIRAITYENAARIFSFDPFEHRAPEACTVGAL
                     RTLAAGVDTSIQPRFQRHEPPTEPLTLLSMLDRASHPLLDDQKASLVAES"
     gene            1731..>2186
                     /locus_tag="VNQ33_01080"
     CDS             1731..>2186
                     /locus_tag="VNQ33_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96718.1"
                     /translation="MSRYPFPIPFGWFQICWPDDVTEQSSYLTHNVGRDLAVSRVEGG
                     FEVRDVEGDRRYPTIERNGLVMFWYHPNDEPPSYEIPTLTEFGGDRAWSEPVRRHHAR
                     INALWQELGETAADTAHIQAHLVEYGAEMGHDGKVMSPAKIVDTCWEGPH"
BASE COUNT          324 a          716 c          795 g          351 t
ORIGIN      
        1 ccgagcgtcg ccgatgacca cccactcctc gccgtcgccg tcgatctcga ggtggtacac
       61 ccggtcgcgg aactcggcgg cggcgtactc ctgcaacccg aacggaggct ccacgatgtg
      121 gccgtcggca tcgacgaagt cgagctcggt cgtgggggcg gtgggctcgg tggtggtcat
      181 cgtgtctccg tcaatcgtgg tcgggcggtc agcggtcggt ggcgtcgcgg tctggtcgcg
      241 ctcggtcggc gcgttcagct ctcggcgacg agcgatgcct tctggtcgtc gagcagcggg
      301 tgcgaggccc ggtcgagcat gctgaggagc gtcagcggct cggtcggcgg ctcgtggcgc
      361 tggaagcgcg gctggatcga cgtgtcgacg ccggcggcca gcgtccggag ggcgccgacg
      421 gtgcacgcct cgggcgcccg gtgctcgaag gggtcgaacg agaagatccg ggcggcgttc
      481 tcgtacgtga tcgcccggat ctcgtcgtcg gggaccccgg tcgcctggag cgacgtccag
      541 agctgctcgg gcgaggtggg ccaggtggag tcggagtgcg ggtagtccgc ctcccagcac
      601 atgttggaca ggccgacctg gtggcggttc tccaggccgt gggtgtcgtc gatgaagcag
      661 gtggcgaacc gctcgcggaa cacgtcgctc gggagctggc cgccgaagtc cacgccggtc
      721 cacaccttgt ggtggcggta cacgtagtcc gcccgctcga ggaagtaggg gatccacccg
      781 gtgccgccct cggagaggac gaactggacc ttcgggaagc gccgcaggac cggcgaccac
      841 aggaggtcgg cggcggtctg gaagatgccg atcgcggtca ggttgagcat cgtcgtgatc
      901 ggcgcctcca tcgaggtgat ggcgacctgg ctcgacgagc cgatgtggat gcacaccgcg
      961 gtgccggtct cctcgcaggc ggcccagaac gggtcccagt gcgggttgtg gaagctcggg
     1021 tggcccagct tctccgggtt ctcggaccag gtgacggcgt ggcagccgag cgatgccagc
     1081 cggcgcacct cggcggccat cagctcggga tcccagtagg ggatgaggcc gagcgggatg
     1141 aagcgccccg gatgggcccc gcaccaatcc tcgaggtgcc agtcgttgta ggccttgagg
     1201 acgacgaggc ccagctcctt gtcctcagag ttgttgaaca tctgcccgca gaactggggg
     1261 aacgacggga agttcatgga ggccaagacg ccgttggcgt tcatgtcgtc gatgcggcga
     1321 tcgacgtcgt aggcgccctc gcggatctcg gcgaagctcg tgggctccac cccgtactcg
     1381 gcgggggggc gcccggcgac cgcgttgagg cccacgttcg ggatgtcctt gccctcgtac
     1441 acccactggt ccgagccgtc gtctttggac accagcttgg gggctcggtc ggcgaaggcg
     1501 gacgggatgc gcccctcgaa catgtccggt ggttcgacca cgtggtcgtc gacggagatc
     1561 aggatcaggt catcgagggt catggcttcg ctccaacgct ggcgtcgggc catcctcgcg
     1621 gacgaggcgc ccggacggcg atctggcaga tgtgacactt tccacctctc gggagccggg
     1681 cgtcgcgacc cgatacgctg tcgcggaccg tcaccctgga gcttgcaccc gtgtcgcgct
     1741 acccgtttcc catcccgttc ggctggttcc agatctgctg gcccgacgac gtcaccgagc
     1801 agtcgtcgta tctgacccac aacgtcggcc gcgacctggc ggtctcgcgc gtcgagggcg
     1861 ggttcgaggt gcgcgacgtc gagggcgacc gccggtaccc gaccatcgag cgcaacggcc
     1921 tcgtgatgtt ctggtaccac cccaacgacg agccgccgtc ctacgagatc cccaccctca
     1981 ccgagttcgg cggggaccgg gcctggtccg aaccggtccg ccgccaccac gcccggatca
     2041 acgcgctctg gcaggagctg ggcgagaccg cggccgacac cgcccacatc caagcccacc
     2101 tcgtcgagta cggcgccgag atgggccacg acggcaaggt catgagcccg gccaagatcg
     2161 tcgacacctg ttgggagggg ccgcac
//
LOCUS       DATMFS010000059         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_71985, whole genome shotgun sequence.
ACCESSION   DATMFS010000059 DATMFS010000000
VERSION     DATMFS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71985"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VNQ33_01085"
     CDS             complement(<1..209)
                     /locus_tag="VNQ33_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96719.1"
                     /translation="MSRDLSDLRESYQRAGLHRAVLADDPIDQFEHWWDEWRSTDPYD
                     AAACVLSTVGADGQPSARFVLCRSY"
     gene            complement(221..535)
                     /locus_tag="VNQ33_01090"
     CDS             complement(221..535)
                     /locus_tag="VNQ33_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96720.1"
                     /translation="MNDPETVVDALRTLKADGYEIEFQLVDGALVCDTDDRACPASDV
                     KVERLYRFEGPSDPGDEMVVFGLRDPESGRRGVLASAFGSAADPQLLEHLHGLSTRFG
                     TD"
     gene            840..1016
                     /locus_tag="VNQ33_01095"
     CDS             840..1016
                     /locus_tag="VNQ33_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96721.1"
                     /translation="MNAAAAVALIALAAAHPSWRFWVALTAVIAGIGELIRWHRARRA
                     GDDGWEPLQPSSPS"
     gene            complement(1026..1400)
                     /locus_tag="VNQ33_01100"
     CDS             complement(1026..1400)
                     /locus_tag="VNQ33_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWJ96722.1"
                     /translation="MSDEPSRIDVKLASARNRIDRVAPGDLAAEVAAGAVVVDLRPDA
                     NRAEEGELPGAVVIERIHLEWRFDRTSGAAIPEASDDLRVILVCNEGYASSLAAADLQ
                     DLGIHRATDLIGGYRAWKAANP"
     gene            complement(1397..1987)
                     /locus_tag="VNQ33_01105"
     CDS             complement(1397..1987)
                     /locus_tag="VNQ33_01105"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HWJ96723.1"
                     /translation="MSIATNLDLDRSTAARRVSLARTNRRKPQPPALDVETCTAIAQG
                     LAQAEDLWRPHVSHDPLSRTSVRLVATDAYEVWLLGWTDGQRVELHDHGGANAAFVVL
                     EGVLDEITLGFAGTTTKRLDPGGVGTVPAGVIHDVVNTAPEVATSLHVYSNPLRTMTY
                     YDADGDPVYTELVEEVPALLTGATQSRALHPARRDR"
BASE COUNT          307 a          768 c          880 g          302 t
ORIGIN      
        1 tcgtacgagc ggcacagcac gaaccgggcc gacggctgcc cgtcggcgcc caccgtgctc
       61 aggacgcacg cggcagcgtc gtaggggtcg gtcgagcgcc actcgtccca ccagtgctcg
      121 aactggtcga tcgggtcgtc ggcgagcacc gcccggtgga ggccggcgcg ctggtacgac
      181 tcgcgcaggt cgctgaggtc tcggctcatc ccggcgcggg tcagtcggtg ccgaagcggg
      241 tggagagacc gtggaggtgc tcgagcagct gggggtcggc ggcggagccg aaggcagagg
      301 ccaacacgcc ccggcggccg gactccggat cgcgcaggcc gaacacgacc atctcgtcgc
      361 cgggatcgct cggcccctcg aaccggtaga gccgctccac cttcacgtcg gacgccggac
      421 acgcccggtc gtcggtgtcg cacacgaggg cgccgtcgac gagttggaac tcgatctcgt
      481 agccatcggc cttcagggtc cgcagggcgt cgacgacggt ctcgggatcg ttcatgggct
      541 cagtctggcg actcccgccg cccttcgctc gcgtcggccc acgatcaccg ccgcggcgac
      601 cgtgccggag gaggtcggcg gtgaggcgac gccaggcgcg gtggggccgc cgacgtccgt
      661 ggcggcggca cgagcggggg cgcgacgact tcggtcacct cgttccgcac gccggcgccc
      721 agatccggat cgagtcgggg ggacctgctc aggatcaccg ttgctcgtgc cgaaggagcg
      781 gtgtggccga tcaccggaga cagcggacga gggcctcgcc gaggacgaag gtgctcgcgg
      841 tgaacgcggc ggctgcggtc gccttgatcg ccctggccgc cgcccacccg tcgtggcggt
      901 tctgggtggc cctcaccgcg gtgatcgcgg ggatcggcga gctcatccgg tggcaccggg
      961 cccgtcgagc gggcgacgac ggctgggagc ccctgcagcc gtcgtcgccc tcctgaggac
     1021 cgcgctcagg ggttggcggc cttccaggcg cggtagccgc cgatgaggtc cgttgcccgg
     1081 tggatgccga ggtcctggag gtcggcggcg gccaggctcg aggcgtagcc ctcgttgcag
     1141 accaggatca cgcggaggtc gtcgctcgcc tcggggatcg ccgcgccgct cgtccggtcg
     1201 aagcgccact cgaggtggat ccgctcgatc accaccgcac cgggcagctc gccctcctcg
     1261 gcgcggttcg catcaggccg gaggtccacc accaccgcac cggcggcgac ctcggcagcc
     1321 aggtcgccag gagcgacccg gtcgatgcgg ttccgagcgg aggccagctt cacgtcgatc
     1381 cgggagggct cgtcgctcat cggtcccgcc tggccgggtg cagcgcccgg ctctgggtcg
     1441 caccggtcag cagggccggg acctcctcga ccagctcggt gtagacgggg tcgccatcgg
     1501 cgtcgtagta ggtcatcgtt cggagggggt tcgagtacac gtggaggctc gtggccacct
     1561 cgggcgcggt gttcaccacg tcgtggatca cgccggcggg cacggtgccg actccgcccg
     1621 ggtccaggcg cttggtggtg gtgccggcga agccgagcgt gatctcgtcg agcacgcctt
     1681 cgagcacgac gaacgcggcg ttggcgccac cgtggtcgtg gagctcgacg cgctggccgt
     1741 cggtccagcc gagcagccac acctcgtagg catcggtggc caccaagcgg acgctggtcc
     1801 ggctgagcgg gtcgtgcgac acgtgcgggc gccagaggtc ctccgcctgg gccaggccct
     1861 gggcgatggc cgtgcaggtc tcgacgtcga gcgccggcgg ttgcggcttc cggcggttgg
     1921 tgcgagcgag cgacacccgc cgggcggcgg tggagcgatc gagatcgagg ttggtggcga
     1981 tggacacggg gcgagcctca gcggaggggg gtccggcaga ccggacggca gcgaaacgga
     2041 acgacgagga aggggtgcag gcagggcggc ggcgcggacg caaggcttcg gccgcgccgc
     2101 cgctcctgca cgccggaatc gacaagggag agccgatccg gacgaccgcc gcctcctgac
     2161 aggggaacca gagggcgggc ggcaggtcag tgcggggagc tcagctccgc cggcaacatc
     2221 gcccgaggca ccgtgcgtgc ggcagccggt ggcgtcg
//
LOCUS       DATMFS010000060         3857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_48894, whole genome shotgun sequence.
ACCESSION   DATMFS010000060 DATMFS010000000
VERSION     DATMFS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48894"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2623)
                     /gene="polA"
                     /locus_tag="VNQ33_01110"
     CDS             complement(<1..2623)
                     /gene="polA"
                     /locus_tag="VNQ33_01110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWJ96724.1"
                     /translation="MRFMLIDGNSLTYRAFHALPTDLVTASGQVTNAVFGFTSMFINL
                     VRDHKPDAIAVAFDRPEKTFRHERDPEYKANRIAAPDILRQQLPLVRQVVDTLGVTAI
                     EQVGVEADDIIATFATLAAERGDDVLIVTGDRDSYQLVQDPHIRVIYNKRGVSDYADY
                     DEAGIVERTGVHPTKYVQYAALRGDNSDNLPGVPGVGEKTAAKLINSYGGLDGIFEHV
                     DEQTPKLRQNLMENEDRARSNVEMMTLMRDVELPISLDDLGFPQPDTDEVRALFDFLE
                     FRTLYDRLAEALEADLGSSSSAVDVLEAEVEVLDQPATAVAALEAAAGATGPLAVAAS
                     WTGAAGQSPVEGLAFVTDTEAGEVAWLPVEVLGDTGVRDAMRAALEERTIAAHPAKPI
                     LRSLLSADDTAPLPKIAIDTTLAAYLLDPAETRYSIEDVLARYAHLELPAGTVAPSGQ
                     LDLGGDTVDVGLLAARHALAVSRLIDPMLASLDANGLRALHDDIETPLVAVLAQMEHV
                     GVGVDRAELEALNERLTAEVSELAAAIQEDAGEPFNVNSTPQLRTILFDKLGLTPTKK
                     TKTGFSTDAASLEKMEGQHPIIEHLLRYREVEKLRSTYGTGLLAEVGPDDRIHATFNQ
                     TVARTGRLSSDAPNLHNIPVRSEQGKLFRKAFVPAPGCELLVADYNQIELRCIAHLAH
                     DPGLLAAFNAGDDIHTATAARVFHVDPADVDGGQRAKAKMVSYGLAYGMEAYGLATRL
                     NISRGEAAEILDAYFAAFPSVKDYMERTVAEARERGYTETLFGRRRPIPELASPNRGI
                     KQAGERQAMNAGIQGLAADIFKVALVRIDARLRELDSGARLVLQVHDEVLLEVPDAER
                     EEIGPLVVDLMRHSAELD"
     gene            complement(2806..3066)
                     /locus_tag="VNQ33_01115"
     CDS             complement(2806..3066)
                     /locus_tag="VNQ33_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWJ96725.1"
                     /translation="MDETEVAWMSEGHCRFYPPAAFFPSDGNGVVAARAICAECPVQE
                     PCLEYALENRIEHGVWGGCSERERRRILKRRRQEAERKLVAS"
     gene            complement(3203..3781)
                     /locus_tag="VNQ33_01120"
     CDS             complement(3203..3781)
                     /locus_tag="VNQ33_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ96726.1"
                     /translation="MPTRVVIAEDEAIIRLDLKETLEEEGYEVVAETGRGDEAVELVR
                     EHNPDVAILDIKMPGLDGLSAAREIAGERRAAVLILTAFSQRDLIERARDAGALAYLV
                     KPFQKADLFPAIEVALGRFRELKGLHDQATSLEEQLETRKVVDRAKGQLMDEGGLTES
                     AAFAFIQKTAMAERQTMKSVAQRVLDGELPTA"
BASE COUNT          530 a         1281 c         1425 g          621 t
ORIGIN      
        1 cgtcgagctc ggcggagtgg cgcatcagat cgacgacgag cggaccgatc tcctcgcgct
       61 cggcgtcggg cacctccagg agcacctcgt cgtgcacctg caggacgagc cgggcgccgc
      121 tgtcgagctc gcgcaggcgg gcgtcgatgc ggacgagggc gaccttgaag atgtcggcgg
      181 ccaggccctg gatgccggcg ttcatcgcct ggcgctcgcc ggcctgcttg atgccgcggt
      241 tgggcgacgc cagttccggg atcggacggc ggcggccgaa gagcgtctcc gtgtagccgc
      301 gctcgcgggc ctcggcgacg gtgcgctcca tgtagtcctt caccgagggg aaggcagcga
      361 agtaggcatc gaggatctcc gccgcctcgc cccgcgagat gttgaggcgg gtggccaggc
      421 cgtaggcctc catgccgtag gccaggccgt acgacaccat cttcgccttc gcccgctggc
      481 ctccgtcgac atcggccggg tcgacgtgga agacccgtgc ggcggtggcg gtgtggatgt
      541 cgtcgccggc gttgaacgcg gcgaggagcc cggggtcgtg cgcgaggtgg gcgatgcagc
      601 gcagctcgat ctggttgtag tcagcgacga gcagctcgca gcccggcgcc gggacgaacg
      661 ccttgcggaa gagcttcccc tgctcggacc ggaccgggat gttgtggagg ttcggcgcat
      721 ccgacgacag gcgtccggtg cgagcgacgg tctggttgaa ggtggcgtgg atgcggtcgt
      781 caggcccgac ctcggccagc agcccggtgc cgtaggtgga ccgcagcttc tcgacctcgc
      841 ggtagcgcag caggtgctcg atgatcggat gctggccctc catcttctcg agcgacgccg
      901 catcggtgga gaagccggtc ttggtcttct tcgtgggtgt gagaccgagc ttgtcgaaca
      961 agatggtgcg cagctgcggc gtggagttga cgttgaacgg ctcccccgcg tcctcctgga
     1021 tggcggccgc cagttccgag acctcggcgg tgagccgttc gttcagggct tcgagctccg
     1081 ctcgatcgac cccgacgccg acgtgttcca tctgtgccag cacggccacc agcggggtct
     1141 cgatgtcgtc gtggagcgct cggagcccgt tggcgtcgag cgaggcgagc atcgggtcga
     1201 tgaggcggga cacagccagc gcgtggcgtg ccgcgagcag gccgacgtcg accgtgtcgc
     1261 cgccgaggtc gagctggccc gacggtgcca ccgtccccgc tggcagctcc aggtgcgcgt
     1321 agcgggcgag cacgtcctcg atggagtagc gcgtctccgc cgggtccagg aggtacgcgg
     1381 cgagcgtggt gtcgatggcg atcttcggca gcggcgccgt gtcgtcggcc gagaggaggc
     1441 tccgcaggat cggcttggcc ggatgggcgg cgatggtccg ctcctcgagc gccgcacgca
     1501 tcgcgtcgcg cacccccgtg tcgccgagca cctccaccgg cagccaggcc acctcgcccg
     1561 cctcggtgtc ggtcacgaag gccagcccct cgaccgggga ctgcccggcg gcgcccgtcc
     1621 acgatgcggc gaccgcgagc gggcccgtgg cacccgctgc ggcctccagc gccgcgaccg
     1681 ccgtcgccgg ctggtcgagc acctcgacct cggcctccag cacgtcgacc gccgagctgc
     1741 tgctgccgag gtcggcctcc agcgcttcgg ccaggcggtc gtacagggtg cggaactcga
     1801 ggaagtcgaa cagggcccgg acctcgtcgg tgtcgggctg cgggaagccg aggtcgtcga
     1861 gcgagatggg cagctcgacg tcgcgcatca gcgtcatcat ctcgacgttg ctgcgtgccc
     1921 ggtcctcgtt ctccatcagg ttctggcgga gcttgggcgt ctgctcgtcg acgtgttcga
     1981 agatgccgtc gagcccgccg tagctgttga tgagcttggc cgccgtcttc tcccccacgc
     2041 cgggcacgcc cgggaggttg tccgagttgt cgccccggag cgcggcgtac tggacgtact
     2101 tggtggggtg gacaccggtc cgctccacga tgccggcctc gtcgtagtcc gcgtagtccg
     2161 atacgccgcg cttgttgtag atcacccgga tgtgaggatc ctggacgagc tggtagctgt
     2221 ctcggtcacc cgtgacgatc aagacgtcgt cgccccgctc ggccgcgagg gtggcgaagg
     2281 tggcgatgat gtcgtcggcc tcgacgccca cctgctcgat ggcggtgacg ccgagcgtgt
     2341 cgaccacctg gcgcacgagg ggcagctgct ggcggaggat gtccggcgcg gcgatgcggt
     2401 tggccttgta ctccgggtcc cgttcgtgtc ggaacgtctt ctccggacgg tcgaacgcca
     2461 cggcgatggc atcgggcttg tggtcccgca cgaggttgat gaacatcgac gtgaagccga
     2521 acaccgcgtt ggtcacctgc ccggaggcgg tgaccaggtc ggtgggcagc gcgtggaacg
     2581 cccggtacgt gagggagttg ccgtcgatga gcatgaaccg catggccgtc gatcgtacgg
     2641 gctggctctg acacccgcga cggccgcccg agggaggggg cggccgtcgc cggggagcag
     2701 gtgtccgctg agcgatgcgt tggacaagca ccgctcagca gacgggcgga cgccacgcgg
     2761 ctgaccgacg cgcgcgcccg cggaggggag cgatccggga ggggatcagc tggcgacgag
     2821 cttgcgctcc gcctcctggc ggcggcgctt gaggatgcgg cggcgctcac gctcggagca
     2881 gccgccccac acgccgtgct cgatgcggtt ctcgagggcg tactcgaggc acggctcctg
     2941 cacggggcac tccgcgcaga tggcacgagc cgccaccacg ccgttgccgt cgctcgggaa
     3001 gaacgcagca ggcgggtaga agcggcagtg cccctccgac atccatgcga cctcggtctc
     3061 gtccatctcg tctcctccag cccgtgcggg gccacccaag gcaccgttgc ctggaggcga
     3121 tgatgcgcga gcccaccgca gagggaggta aagccggaga acttcttcgc agccgcaggt
     3181 caccggcccc ggaagggcgg gatcaggcgg tggggagctc gccgtcgagc acccgctggg
     3241 ccacggactt catggtctgg cgctcggcca tggccgtctt ctggatgaag gcgaaggcag
     3301 cggactcggt gagcccgccc tcgtccatca gctggccctt ggcccggtcg acgaccttgc
     3361 gggtctccag ctgctcctcc agggaggtgg cctggtcgtg gaggcccttc agctcccgga
     3421 agcggcccag ggcgacctcg atggccggga acaggtccgc cttctggaac ggcttcacca
     3481 ggtaggcgag ggcgccagcg tcacgggccc gctcgatgag gtcccgctgg ctgaaggcgg
     3541 tgaggatcaa gacggcagcg cggcgctcgc cggcgatctc ccgagcagcc gagagcccgt
     3601 cgagccccgg catcttgatg tcgaggatgg cgacgtcggg gttgtgctcg cggaccagct
     3661 ccaccgcctc gtcgccgcgg ccggtctcgg ccaccacctc gtagccctct tcctcgaggg
     3721 tctccttgag atcgaggcgg atgatcgcct cgtcttccgc gatcacgaca cgggtgggca
     3781 ccgttgcgct ccagtgctgg ggtagtcagg tggtggcgag cagccggtcc agcagctcgt
     3841 cttgggttcg ttcgagt
//
LOCUS       DATMFS010000061         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_304783, whole genome shotgun sequence.
ACCESSION   DATMFS010000061 DATMFS010000000
VERSION     DATMFS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304783"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VNQ33_01125"
     CDS             <1..226
                     /locus_tag="VNQ33_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96727.1"
                     /translation="AGRRRRVQEGVVDHPGELGTFHRGILPARPPAPTRRLPRSDLDV
                     VCTSPEPALATAFDGVHTCSTSAVRIMVGC"
     gene            220..843
                     /gene="upp"
                     /locus_tag="VNQ33_01130"
     CDS             220..843
                     /gene="upp"
                     /locus_tag="VNQ33_01130"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWJ96728.1"
                     /translation="MLTTVVDHPLAHQALTTLRDQDTDRAAFRVAMDDLAGILVYEAT
                     RQLRVETFDVTTPLAVTRGIRVTEPPMAVPILRAGLGLLPGVLRYLPDCPTGFIGVAR
                     NEETLVPEPYMNSVPADLHRRDVLVLDPMLATGGSLTYACGLLRDRNPGRLVAVCVLA
                     APEGVAALDRSGLCDELVVGSIDDHLNDVSYIVPGLGDAGDRLYGTA"
     gene            988..1854
                     /locus_tag="VNQ33_01135"
     CDS             988..1854
                     /locus_tag="VNQ33_01135"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ96729.1"
                     /translation="MTLHIESFTVDARDPLALATWWSEAIGWVICSESEDGVEVDLCE
                     RLDPDGGHPYPELSFVGDGGHGEGQEKIHLDLNSFSTGDQHATVERLIGMGATRADVG
                     QAPDAPFTVLADPEGNHFCVLDPRPEYAHLGSVAGYTVAAHDAAALRDVWATATGWDV
                     TRDEPDHVVLAPPDGSAPIEIVTRPTMPPQRRQGAHPPRRRPRRPRRPGGGGRPAPRP
                     RRHPRRHRPDRRRALGRPRRPRGQRALRPLTPPLSPLPQVDRDGWHRRRPPAPATPTA
                     TATDFQLGGSRS"
     gene            complement(1996..>3088)
                     /locus_tag="VNQ33_01140"
     CDS             complement(1996..>3088)
                     /locus_tag="VNQ33_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96730.1"
                     /translation="RVGDELVQAYVAAASQVGVVPSERSVVAAYTAMHGVGAGTIRRA
                     FAAAGFAPVVEVEQQVEPDPDFPTVAFPNPEEPGALDLGIATAAAAGADILLANDPDA
                     DRLGAAVPDPTAPSRWRSLRGDEIGAVLAHHLVSSGGIADDAVLATTIVSSTLLGKIA
                     AAAGRPFVETLTGFKWLGRAADPGGQLGFAYEEALGFCAGGLVRDKDGVTAALLLAEA
                     AAVQKRDGRTLLHVLDDLAVEHGVHQTVQWSARVAGTDAMDQMTAAMDRMRRTPPTEL
                     AGRAVQRIDDLIDGDAARGFAPSDVLTWYLADARIVVRPSGTEPKLKAYVEVVEPVAE
                     RGDLVAARKRATAALEQLVAATAAATGLA"
BASE COUNT          426 a         1224 c         1038 g          400 t
ORIGIN      
        1 ggccgggcgt cgccgccggg tccaggaggg tgtggtcgat catccgggcg agctcggcac
       61 gttccatcgg ggcatcctgc cagcccggcc gcccgctccc acccgccgcc tcccccgctc
      121 cgacctggac gtcgtgtgca ccagtcccga accggcgctg gccacggcgt tcgacggggt
      181 tcacacctgc tcgacgtccg ctgtgcgcat catggtcggg tgctgaccac cgtcgtcgac
      241 cacccgctgg cccaccaggc cctgaccacg ctgcgcgacc aggacaccga ccgggccgcg
      301 ttccgcgtgg ccatggacga cctcgccggc atcctcgtct acgaggccac ccgccagctc
      361 cgggtcgaga cgttcgacgt caccaccccg ctcgcggtca cccggggcat ccgcgtcacc
      421 gagccgccga tggccgtgcc gatcctgcgg gccggcctcg gcctgctgcc cggcgtgctc
      481 cgctacctgc ccgactgccc cacggggttc atcggcgtcg cccggaacga ggagaccctg
      541 gtccccgagc cgtacatgaa ctcggtgccc gctgacctcc accggcgcga cgtcctcgtg
      601 ctggacccga tgctcgccac cggcggctcg ctcacctacg cctgcggcct cctccgcgac
      661 cgaaaccccg gccgcctcgt cgcggtgtgc gtgctggcgg cccctgaggg cgtggcggcg
      721 ctggaccgca gcggcctctg cgacgagctg gtggtcggct ccatcgacga ccacctcaac
      781 gacgtctcct acatcgtccc cgggctcggc gacgccggcg accgcctcta cggcacggcc
      841 tgatccgacc catcgggact gccgcaccac cgcgatcacg tgtgcgtgac gagtggctcg
      901 ccctcggcgc cccgttctac ccatatcggg actgccgcac cgccgcgatc acgtgtgcgc
      961 cggcacgcag ccgggtacgc cgtaggcatg acgctgcaca tcgagagctt caccgtcgac
     1021 gcccgcgacc cgctggccct cgccacctgg tggtccgagg cgatcggctg ggtgatctgc
     1081 tccgagtcgg aggacggcgt cgaggtggac ctctgcgagc ggctcgaccc cgacggcggc
     1141 catccgtacc cggagctgtc gttcgtgggc gacggcggcc acggcgaggg ccaggagaag
     1201 atccacctgg atctgaacag cttctcgaca ggcgaccagc acgccacggt cgaacgactg
     1261 atcgggatgg gagccacccg cgccgacgtc gggcaggcgc ccgacgcgcc cttcaccgtg
     1321 ctcgccgacc ccgagggcaa ccacttctgc gtcctcgacc cccggcccga gtacgctcac
     1381 ctggggtcgg tcgccggcta cacggtcgcc gcccacgatg ccgccgccct gcgggacgtg
     1441 tgggccaccg ccactggctg ggacgtcacc cgcgacgagc ccgaccacgt ggtcctggcc
     1501 ccacccgacg ggagcgcccc gatcgagatc gtcacccgac cgacgatgcc cccccagcga
     1561 cgccaaggcg cgcatccacc tcgacgtcgc ccccggcgcc cacgacgacc aggcggaggc
     1621 ggtcgaccag ctcctcgccc tcggcgccac ccacgccgac atcggccaga ccggcgacga
     1681 gccctgggtc gtcctcgccg accccgaggg caacgagctc tgcgtcctct caccccgccc
     1741 ctgagcccgc taccccaggt cgaccgtgac gggtggcacc gacgacgacc acccgcccca
     1801 gcaaccccca ccgccaccgc caccgacttc caacttggag gatcgcggtc gtagagcgcc
     1861 aacatcaccc aagttggtgg atggtggcgc aacgtggagg atcgtggtcg catacgggca
     1921 ccgatcctcc aacctcgccg tcaccgatcc tccaacctcg ccgtcaccga tcctggccgg
     1981 ggccggcagc cctcgtcagg cgaggccggt ggcggcggcg gtcgccgcca ccagttgctc
     2041 caacgcagcg gtcgcccgct tccgggccgc gaccagatcc ccccgctcag ccacgggttc
     2101 gacgacctcg acataggcct tgagcttggg ctcggtgccg ctggggcgga cgacgatgcg
     2161 ggcgtcggcg aggtaccagg tcaagacatc gctcggcgcg aagccacggg cggcatcgcc
     2221 gtcgatgagg tcgtcgatcc gctggacggc tcgaccggcc agctcggtgg gcggcgtgcg
     2281 ccgcatccgg tccatcgccg cggtcatctg gtccatggcg tcggtgccgg cgacgcgggc
     2341 cgaccactgc acggtctggt ggacgccgtg ctccacggcc aggtcatcga gcacgtggag
     2401 cagggtccgc ccgtcgcgct tttgcaccgc tgccgcctcg gcgagcagca gggcggcggt
     2461 gacgccgtcc ttgtcgcgca ccaggccgcc ggcgcagaac ccgagcgcct cctcgtaggc
     2521 gaagccgagc tggccaccgg ggtcggcggc ccggcccagc cacttgaagc cggtcagcgt
     2581 ctcgacgaac ggccgcccgg ccgccgcggc gatcttgccg aggagcgtcg acgacacgat
     2641 cgtggtggcc agcaccgcgt cgtcggcgat gccgcccgac gacacgaggt ggtgggcgag
     2701 gaccgcgccg atctcatcgc cccgcaagga ccgccatcga gacggggccg ttgggtccgg
     2761 taccgccgcg ccaaggcggt cggcgtcggg gtcgttggcc aggaggatgt cggccccggc
     2821 ggcggcagcc gtggcgatcc cgaggtcgag ggcaccgggc tcctcggggt tggggaaggc
     2881 gacggtcggg aagtccggat ccggctccac ctgctgctcc acctcgacca ccggcgcgaa
     2941 cccggcggcg gcgaacgccc ggcggatggt gccagcgccg acgccgtgca tcgccgtgta
     3001 ggccgccacg accgaccgct cggacggcac cacgcccacc tgggacgcgg cggccacgta
     3061 ggcctgcacc agctcgtcgc cgacccgc
//
LOCUS       DATMFS010000062         4799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_164875, whole genome shotgun sequence.
ACCESSION   DATMFS010000062 DATMFS010000000
VERSION     DATMFS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164875"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VNQ33_01145"
     CDS             complement(<1..377)
                     /locus_tag="VNQ33_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWJ96731.1"
                     /translation="MARTIDVLGDWAAGTGPLYRRLERALAGAVERGDLAPGWRLPSE
                     RALAPAIAVSRGVVVAAYDRSVEQGTVARRPGSGTFVAAPAGGALPPGREGSRLVRRF
                     VDRSQDRPAIDLSISVVAAPLGVA"
     gene            495..1667
                     /locus_tag="VNQ33_01150"
     CDS             495..1667
                     /locus_tag="VNQ33_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWJ96732.1"
                     /translation="MVKSLAVERAGSSAIRDLLEITEQPEIVSLAGGLPNPATLPTDA
                     IARAAAAALEDDPLGALQYGPTEGYRPLRRWVAGRHGLAADPHGEEHVLVTSGSQQAL
                     ELLTRALVDPGGLVALADPAYVGALQALRAAGVDLAAIPADGDGLCVDALADRLATGE
                     RPALVYVVANFDNPSGSTLSAERRVALAALADRYGFWIVDDDPYGDLRWHGEAPVPLR
                     ELTDRTITLGSASKVLAPGLRVGWAVAPVEVRRAMTTLKQSADLHTSALTQQIVHRTL
                     AEPGFLEGHLVTLRTTYQAQAEHLVGALRTKLGDRMTVPEPDGGMFVWAELLDTTADS
                     AQLLDPAVAAGVAFVPGAAFGVERLHPTRMRLSYATADAASLDLAVDRLAGVIPAP"
     gene            1704..2891
                     /locus_tag="VNQ33_01155"
     CDS             1704..2891
                     /locus_tag="VNQ33_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWJ96733.1"
                     /translation="MDLTVIAIPAYFSTMGAEYAYLKRQAQDRAPTAGDYTRDDTLAS
                     LAMGVGSLLAPLTLKKLVEPVTPGRGKYGKVLVAGALGAAVATTVADAVARRQEAGEL
                     PEAGTIPDGPAAAQRPTGRKAKRGLARRIASSTGVAAVTGGVLAASTMWASKTAAQRI
                     FARHDRDLGDGVLANLGAIVGWDFIYYWNHRFMHETRWLWAIHVVHHSSEHYNLSTAL
                     RQPVLDVFGMFVPYGALSLAGFRPDTIETARGVNLLYQYWIHTETIGKLGPIEEVFNT
                     ASHHRVHHGSNRQYLDRNHGSILIIWDRLFGTFEREDEPVRYGLTKNIDTYNPLKIAT
                     HEHADILRDVAASTTWKDRLQFVFRGPGWAYDRHKETAEAVVHGDVVDTVATPTVTAA
                     GVA"
     gene            2976..3782
                     /locus_tag="VNQ33_01160"
     CDS             2976..3782
                     /locus_tag="VNQ33_01160"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWJ96734.1"
                     /translation="MVDARVTPSPLTVALHRFDVPEAYISDALDLLPPHCEAAMIYGS
                     RARGDHRPDSDLDVLALVDSPRGSRSSGRVSVSCYTGAQLRSASGSLFGMHLRRDGKV
                     LIDPDGVLQAILHDLRDPDPQDLFDRVNHFSALLGVDRADARNHLPGLVRLGRYLLRT
                     AVYAEAIKTGNPCFSVAELAVRFEDPALEQILDSSPLANPDPTADDLSDLRRRLERWT
                     GPSPSAPWSTLAQWAVAEWSTDRMRATLATLALGGSEEPFDYSELPKVTL"
     gene            3779..4396
                     /locus_tag="VNQ33_01165"
     CDS             3779..4396
                     /locus_tag="VNQ33_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ96735.1"
                     /translation="MTEALGFIGDIHGESKLLTELLPVALLRARRLVFLGDYVDRGPD
                     SFGVLEILCNLAESKEGHVFLRGNHDDAMADAIDHGRVDQYLRQGGAGTVAQYVQHPE
                     PDLTAQFAASVPARHRKFLHELQDSFAAHDVSASHVPPRSSGSIATFHVSAHRPHGSF
                     EPLVSDREAWIDTGAGTMPGGRLTCLFWPSMAWIQTTPATVSAAF"
     gene            complement(4360..>4799)
                     /locus_tag="VNQ33_01170"
     CDS             complement(4360..>4799)
                     /locus_tag="VNQ33_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96736.1"
                     /translation="NARLRNSDGESISTLQLGRIELATGDLPSAQEHADACYAINLRS
                     GNVEGLVIAEHLGGQVALAKQEPDVAVRVLRSALSKAVELGSPSRESLVRSDLAIALA
                     QTGIGGAEAAAEVDRALVLATRSGDAFAHTAAQKAAETVAGVV"
BASE COUNT          744 a         1828 c         1512 g          715 t
ORIGIN      
        1 gcgacgccca gcggagcggc caccacggag atcgacaggt cgatggcagg acggtcctgg
       61 ctgcggtcca cgaagcggcg cacgaggcgc gacccttcgc ggccgggcgg cagcgcacca
      121 ccggccggcg cggcgacgaa cgtcccgctc cccggccgcc gggccacggt gccctgctcg
      181 accgatcggt cgtaggcagc aacgaccacg ccgcggctga cggcgatggc cggcgccagc
      241 gcccgctccg acggcaggcg ccacccgggt gcgagatcgc ctcgctcgac ggccccagcg
      301 agcgcccgct cgagccgccg gtacagcggg cccgttccgg cggcccagtc gcccaggacg
      361 tcgatcgtcc gcgccatcgc cgccgcccgg ccctcggcgc tcgagcccgg gccccctccc
      421 gaccccgagg atggtgccac cccggagaaa gtggctctgt ttgccatggt ccacttctgt
      481 cagagttcgc gaccatggtc aagtccctcg ccgtcgagcg cgccggctcc tccgccatcc
      541 gggatctgct cgagatcacc gagcagcccg agatcgtgtc gctcgcgggc gggctgccga
      601 acccggccac gctcccgacc gacgccatcg cccgagccgc cgcggccgcc ctggaggacg
      661 atccgctggg tgcgctccag tacggaccca ccgagggcta ccgcccgctg cgccgatggg
      721 tcgctggccg ccacggcctc gccgccgacc cgcacgggga ggagcacgtc ctcgtgacca
      781 gcggctccca gcaggcgctc gagctgctga cccgcgccct ggtcgatccc gggggtctcg
      841 tcgccctggc cgatccggcc tacgtcggcg ccctccaggc gctccgggcc gccggcgtgg
      901 atctggccgc catccctgct gacggcgacg ggctgtgcgt cgacgccctg gccgaccgct
      961 tggcgaccgg cgagcggccg gcgctcgtct acgtcgtcgc caacttcgac aacccgagcg
     1021 ggtcgacgct gagcgccgag cgccgcgtcg ccctggccgc gctggcggac cgctacgggt
     1081 tctggatcgt ggacgacgac ccgtacggcg acctccgctg gcacggcgag gcacccgtcc
     1141 ccctgcgcga gctgaccgat cgcaccatca ccctcgggtc ggcgtcgaag gtcctcgccc
     1201 ccggcctccg ggtcggctgg gccgtcgcgc cggtcgaggt ccgccgggcc atgacgaccc
     1261 tcaagcagtc cgccgacctc cacacgagtg cgctgaccca gcagatcgtc caccggaccc
     1321 tggccgagcc cgggttcctc gaaggccacc tcgtcaccct ccgcaccacg taccaggccc
     1381 aggccgagca cctggtgggt gcgctgcgaa cgaagctcgg cgatcggatg accgtgccgg
     1441 agcccgacgg cggcatgttc gtctgggcgg agctcctcga caccacggcg gacagcgccc
     1501 agctgctcga ccccgccgtc gccgcgggcg tggcgttcgt cccgggcgcc gccttcggcg
     1561 tcgagcgcct ccaccccacc cggatgcgcc tgtcctacgc caccgccgat gccgccagcc
     1621 tcgacctggc cgtcgaccgg ctcgctgggg tgatcccagc cccctgaggc agaatggccc
     1681 gtacgctctg cgaggggacg cacgtggatc tcaccgtcat cgccatcccg gcgtacttca
     1741 gcaccatggg tgccgagtac gcctacctca agcgccaggc gcaggaccgc gcccccaccg
     1801 ccggcgacta cacccgcgac gacacgctgg ccagcctggc catgggcgtc ggcagcctcc
     1861 tcgccccgct gacgctgaag aagctggtcg agccggtcac gccgggcagg ggcaagtacg
     1921 gcaaggtcct cgtggcgggc gcgctcggcg ccgcggtggc caccaccgtc gccgatgccg
     1981 tcgcccgtcg ccaggaggcc ggcgagctgc ccgaggccgg cacgatcccc gacggccccg
     2041 ccgccgccca gcgtcccacc ggccgcaagg caaagcgcgg tctcgcccgc cgcatcgcct
     2101 cgtccaccgg cgtggccgcc gtgaccggag gcgtcctcgc cgccagcacc atgtgggcca
     2161 gcaagacggc ggcgcagagg atcttcgccc gccacgaccg cgacctcggc gacggcgtgc
     2221 tcgccaacct cggcgccatc gtcggctggg acttcatcta ctactggaac caccggttca
     2281 tgcacgagac ccggtggctg tgggcgatcc acgtcgtcca ccacagctcc gagcactaca
     2341 acctgtccac cgcgctccgg cagccggtgc tcgacgtgtt cggcatgttc gtcccctacg
     2401 gggcgctctc gctggcgggc ttccggcccg acaccatcga gaccgcccgc ggcgtgaacc
     2461 tgctctacca gtactggatc cacaccgaga cgatcgggaa gctcgggccg atcgaagagg
     2521 tgttcaacac cgcctcgcac caccgggtgc accacgggtc gaaccgccag tacctcgacc
     2581 gcaaccacgg cagcatcctc atcatctggg atcgcctgtt cggcacgttc gagcgggagg
     2641 acgaaccggt gcgctatggc ctcaccaaga acatcgacac gtacaacccg ctcaagatcg
     2701 ccacccacga gcacgccgac atcctccgcg acgtcgccgc ctccaccacg tggaaggacc
     2761 gcctgcagtt cgtgttccgc ggtccgggct gggcctacga ccgccacaag gagacggccg
     2821 aggcggtcgt ccacggcgac gtcgtcgaca cggtggccac gcccaccgtc accgccgcag
     2881 gcgtggcctg acgcatcgag cggtccgaga atccgtgggt gggcggacgg caaacactca
     2941 gatcgctgtg atccgcggac gagtaccgtg aaggcgtggt cgacgcccga gtcacgccca
     3001 gtccgctcac agtggccctg cataggttcg atgtccccga ggcctacatc agcgacgccc
     3061 tcgacctcct tccaccacac tgcgaagcag cgatgatcta cggctcccga gcccggggcg
     3121 accaccggcc cgattccgat ctcgatgtgc tcgcgttggt ggactctcca cgcggctccc
     3181 gctcctcagg tcgagtctct gtcagctgct acacgggggc tcaactacga agtgcgtccg
     3241 gcagtctctt cggcatgcac cttcgccgcg atggcaaggt cctcatcgat ccggacggcg
     3301 tcctccaggc gatccttcac gatctccgtg acccagatcc ccaggatctc ttcgaccgcg
     3361 tcaaccattt ctctgcgctg ctcggggtcg accgcgcgga tgcaaggaac cacctgccgg
     3421 ggctggtccg cctcgggcgg tacctgctgc gaacggcggt ctacgccgag gccatcaaga
     3481 cgggcaaccc gtgcttctcc gttgccgagt tggccgtccg gttcgaggac ccagccctcg
     3541 aacagatcct cgattcctca ccgttggcga accccgaccc gaccgccgac gacctttccg
     3601 acctacgacg acgactcgaa cgatggaccg gtccatcgcc ctctgcaccg tggtcgacgc
     3661 tcgctcagtg ggcagttgct gaatggtcga ccgatcgcat gcgcgcgacg ttggcgacgc
     3721 tcgcgctcgg cggctccgaa gaacctttcg actattccga gcttcccaag gtgacgctgt
     3781 gaccgaagcg ttgggattca tcggcgacat ccacggtgaa tcgaagctgc tgacggagct
     3841 cctgcccgtg gcgctgcttc gagcgcgacg gctggtgttc ctcggcgact acgtcgaccg
     3901 aggccccgac tcgtttggag tacttgagat cctgtgcaac cttgccgagt caaaggaggg
     3961 ccacgtgttc cttcgaggta atcacgacga tgcgatggca gatgcaatcg atcacggtcg
     4021 ggtcgatcag tacctacggc agggcggcgc agggacggtt gcccaatacg ttcaacaccc
     4081 ggagccagac ctcaccgccc agttcgcggc gtcggttccg gcccggcatc gcaagttcct
     4141 gcatgaactt caagacagct tcgcggcaca tgacgtcagc gcctcgcacg tcccacctcg
     4201 ctcatcgggc agcatcgcca ccttccatgt gtcagcccac cgcccccatg gcagcttcga
     4261 accgctcgtt tcagatcggg aagcctggat cgacaccggt gccggcacga tgcccggcgg
     4321 ccgcctgacc tgcttgttct ggccatcgat ggcttggatt cagacgacgc ccgcaacggt
     4381 ctcagccgcc ttctgagcag cggtgtgcgc gaacgcgtca ccggatctgg tcgcgagcac
     4441 gagggctcgg tccacctctg ccgcggcctc ggcaccgccg atccccgtct gggccagcgc
     4501 tatcgccagg tcggacctga ccagcgactc acgagacgga ctccccaact cgacggcttt
     4561 cgaaagggca gatcgaagga ctcgaacagc aacatctggc tcttgcttcg cgagagcgac
     4621 ctgacccccg aggtgctcgg cgatcacgag gccctctacg ttcccagatc gcagattgat
     4681 ggcgtagcac gcgtccgcat gctcctgagc cgacggtaga tcgcctgtag cgagttcgat
     4741 acgtccgagc tgaagcgttg aaatgctctc gccatccgag ttcctgagcc gtgcattcg
//
LOCUS       DATMFS010000063         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_164919, whole genome shotgun sequence.
ACCESSION   DATMFS010000063 DATMFS010000000
VERSION     DATMFS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164919"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VNQ33_01175"
     CDS             complement(<1..866)
                     /locus_tag="VNQ33_01175"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWJ96737.1"
                     /translation="MAAPTSDVEDPAPAPRFRTGGMVPSAWALPDVLPTDRLSATGMA
                     RPAVREPLRRIASWRNVLTVAALWVTIAALFGVASWLDHPLGYASLFVLMGPMFARLA
                     ILGHEAAHRLLFRNKKANDLVGRWLLDYPAFVPFDVYRRVHFAHHKDEFGPNEPDIPL
                     YAGYPVPAASWRRKLLRDATGSSGWKNLKPLFTSWKSKPARPVVARIWATQLVLWAVI
                     WIGTGHGWLYPLCWLGPWLTVWRVLNRLRAIAEHGGMERSKDRRRTTHHIRQSWAARF
                     WIVPYRTGWHLA"
     gene            893..1732
                     /locus_tag="VNQ33_01180"
     CDS             893..1732
                     /locus_tag="VNQ33_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWJ96738.1"
                     /translation="MASRRNTPDRRDRSDRSDRRARPDRPRLTARSVIASTLLGVDPP
                     ELPTRSLVASAELLGVTAGTARVGMSRMVAAGELEATGDGYRLAGHLLARQARQAQSR
                     TGTPDGWDGAWRTALVPGEARAATDRADLRRAMSALRYGELRDGVWLRPDNLPAGALA
                     SAQAVAAAQTIALVGPLDPGTDPVVLARSLWDLDGWAETAAALVDELAPLQERLDHDD
                     ATALAEGFVVSAAVLRHLQADPLLPAALLPDHWPGTRLRSREDRFDHTFKAVLRTWLL
                     ARA"
     gene            1779..3137
                     /locus_tag="VNQ33_01185"
     CDS             1779..3137
                     /locus_tag="VNQ33_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ96739.1"
                     /translation="MAHRRPPGAPRLPALFGLLAVVLLGTWWAAPPAGAQSEADAARC
                     AFTKPEAIEANGGPLGNAIWGRLCQVQDGKTAFPEGVEITVSQGGKQVASGTTGDGGT
                     FVIEIPGNGTYQVSLDTETLPKGFALTDEDRATLDQVNVNLGDQQVAFRLGEDTRGTR
                     DLEDYLTTAAKGLRLGLILAVAAVGLSLVYGVTGLINFAHAELVTFGAIVAFVLNDAG
                     MPFWLCIPFAIGAGCVAGYANDRLLWRPLRKRNMAMVSQMVVSIGLSIALRNIFLIAF
                     GPNSKRYSAASGQREKDFGLVRLTPNDLIIMGICVVVLILVTLMLRKTRLGTAIRATA
                     DNPDLAASSGIGVNRIIAIVWIICGGMAALGGILYGLSVTVSSDMGFILLLSMFAAVV
                     LGGLGNAYGALFGAVILGVMQETSGLFVDTAYKFVVALAVLIVVLLVRPQGLLGTKER
                     FG"
     gene            3134..>3504
                     /locus_tag="VNQ33_01190"
     CDS             3134..>3504
                     /locus_tag="VNQ33_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ96740.1"
                     /translation="MSWLGVFLLALQTALGFPAASYALAATGLNLQFGYTGLSNFGMV
                     AFLMMGAYGMAISADRGWSIWVGFALGIGAAVALALLLGIPTLRLRADYLSVVTIATA
                     EILRLVIAARPSAELTGAARG"
BASE COUNT          465 a         1308 c         1211 g          520 t
ORIGIN      
        1 tgggcgaggt gccagccggt gcggtacggc acgatccaga accgggcggc ccagctctgg
       61 cggatgtggt gcgtggtgcg gcggcggtcc ttgctgcgct ccatgccgcc gtgctcggcg
      121 atggcccgaa gccggttgag cacccgccac acggtgagcc acgggcccag ccagcagagc
      181 gggtagagcc acccgtggcc ggtgccgatc cagatcacgg cccagagcac gagctgcgtc
      241 gcccagatgc gggcgacgac gggccgcgcc ggcttcgact tccacgacgt gaagagcggc
      301 ttgaggttct tccaccccga gctgccggtg gcgtcgcgca ggagcttgcg ccgccacgac
      361 gccgccggga ccgggtagcc ggcgtagagc gggatgtcgg gctcgttcgg cccgaactcg
      421 tccttgtggt gggcgaagtg cacccggcgg tacacgtcga aggggacgaa ggccgggtag
      481 tcgaggagcc accgccccac gaggtcgttc gccttcttgt tgcggaacag cagccggtgg
      541 gccgcctcgt ggccgaggat ggcgagccgg gcgaacatcg ggcccatcag cacgaacagc
      601 gaggcgtacc cgagtggatg gtcgagccag gaggccacgc cgaacagggc ggcgatcgtc
      661 acccacagcg cggcgacggt gagcacgttg cgccagctgg cgatgcggcg cagcggctcg
      721 cgcacggcgg gccgcgccat gccggtggcc gacaggcggt cggtgggcag cacgtcgggc
      781 agcgcccagg cggacgggac catgccgccc gtgcggaacc gaggtgccgg cgcggggtcc
      841 tcgacgtcgc tggtgggtgc ggccataccc acatgttacg ttcaacgatc agatggcgtc
      901 cagacgtaac accccggacc gacgtgaccg gtcggaccgg tcggaccggc gagcccgccc
      961 cgaccggccg aggctcaccg cccggtccgt catcgcgtcg acgctgctcg gcgtcgatcc
     1021 gcccgagctg ccgacccggt cgctcgtggc cagcgccgag ctcctcggcg tcaccgccgg
     1081 aaccgctcgc gtcggcatgt cccggatggt ggcggccggc gagctcgagg ccaccggcga
     1141 cggctaccgg ctcgccgggc acctcctcgc ccgccaggcc cgccaggccc agtcgcgcac
     1201 cggcaccccc gacggctggg acggcgcctg gcggacggcg ctggtccccg gcgaggcccg
     1261 cgcggcgacc gaccgagccg acctgcgccg ggccatgagc gccctgcgct acggcgagct
     1321 gcgcgatggc gtctggctcc ggcccgacaa cctcccggcc ggcgcgctcg cctcggctca
     1381 ggcggtcgct gccgcccaga ccatcgcgct cgtcggcccg ctggacccgg gaacggatcc
     1441 cgtcgtgttg gcccggtcgc tgtgggacct cgatggctgg gcggagacgg cggcggcgct
     1501 ggtcgacgag ttggcgccgc tccaggagcg cctcgatcac gacgacgcca ccgcgctcgc
     1561 cgaggggttc gtcgtctcgg cggcggtgct ccgccacctc caggccgacc cgctgctgcc
     1621 cgccgcgctc ctccccgacc actggccggg cacgcggctg cgttcgcggg aggaccgctt
     1681 cgaccacacc ttcaaggcgg tgctgcggac ctggctcctg gcccgcgcct gacgtcttcc
     1741 tcacctcagg acatgcggat cggtagtgtc ccggccacat ggcacaccga cggccgcctg
     1801 gcgctccccg actccccgcg ctgttcgggc tgctcgcggt cgtcctgctg ggcacgtggt
     1861 gggcggcgcc gcccgccgga gcgcagtccg aggccgatgc cgccagatgc gcgttcacca
     1921 agcccgaggc catcgaggcc aacgggggtc cgctcggcaa cgccatctgg ggtcgcctct
     1981 gccaggtgca agacggcaag acggcgttcc ccgaaggcgt ggagatcacg gtgagccagg
     2041 gtggcaagca ggtggcctcc ggcaccaccg gcgacggcgg aaccttcgtc atcgagatcc
     2101 ccggcaacgg cacctaccag gtcagcctcg acaccgagac cctcccgaag ggcttcgccc
     2161 tcaccgacga ggaccgggcc acgctcgacc aggtgaacgt caacctcggc gaccagcagg
     2221 tggcgttccg cctcggcgag gacacccgcg gcacccgcga cctcgaggac tacctgacca
     2281 cggcggccaa gggcctccgc ttgggcctga tcctcgccgt cgccgccgtc ggcctgtcgc
     2341 tggtctacgg cgtcaccggt ctcatcaact tcgcccacgc cgagctggtc acgttcggtg
     2401 ccatcgtcgc gttcgtcctc aacgacgccg gcatgccgtt ctggctgtgc atccccttcg
     2461 ccatcggcgc cggctgcgtc gccggctacg cgaacgaccg cctgctctgg cggccgctgc
     2521 gcaagcgcaa catggcgatg gtctcgcaga tggtcgtgtc gatcggtctc tcgatcgcgc
     2581 tgcgcaacat cttcctgatc gcgttcggcc cgaacagcaa gcgctactcc gcggcatcgg
     2641 gccagcggga gaaggacttc ggcctggtcc ggctcacgcc caacgacctg atcatcatgg
     2701 ggatctgcgt ggtggtgctg atcctcgtca ccttgatgct gcgcaagacc cgcctcggca
     2761 ccgccatccg ggcgacggcc gacaacccgg acctggcggc atcgtcaggc atcggggtca
     2821 accgcatcat cgccatcgtg tggatcatct gcggcggcat ggccgccctc ggagggatcc
     2881 tctacggcct gtcggtcacg gtctcgtcag acatgggctt catcctgctg ctgtcgatgt
     2941 tcgccgccgt cgtgctcggt ggcctcggca acgcctacgg cgccctgttc ggcgccgtca
     3001 tcctcggcgt catgcaggag acctcgggcc tcttcgtcga cacggcgtac aagttcgtcg
     3061 tcgccctcgc cgtgctcatc gtggtgctgc tggtgcgccc gcaaggcctc ctcgggacga
     3121 aggagcggtt cggatgagct ggctcggcgt cttcctcctc gcgctgcaga cggccctcgg
     3181 cttccccgcg gcgtcgtacg cgctggccgc caccggcctc aacctgcagt tcggctacac
     3241 gggcctgtcc aacttcggca tggtcgcctt cttgatgatg ggcgcctacg gcatggccat
     3301 ctcggcggac cggggctgga gcatctgggt cggcttcgcc ctcggcatcg gcgccgccgt
     3361 cgccctcgcg ctgctgctcg gcatcccgac gctgcgcctc cgtgccgact acctgtcggt
     3421 cgtcaccatc gccaccgccg agatcctccg cctcgtcatc gcggcgcgcc ccagcgccga
     3481 gctcaccgga gccgctcgcg gcat
//
LOCUS       DATMFS010000064         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_95721, whole genome shotgun sequence.
ACCESSION   DATMFS010000064 DATMFS010000000
VERSION     DATMFS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95721"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..131
                     /locus_tag="VNQ33_01195"
     CDS             15..131
                     /locus_tag="VNQ33_01195"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96741.1"
                     /translation="MRVAVSGRSVGPPLWQSLAVLGRERTLARLHAMQAKLP"
     gene            134..772
                     /locus_tag="VNQ33_01200"
     CDS             134..772
                     /locus_tag="VNQ33_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWJ96742.1"
                     /translation="MASSDQDEPAVPKARWLRRTLWTAVILLGVVVLYLGVTFVQVWQ
                     ASRSDGARKADAIVVLGAAQYNGTPSPALQNRLDHALDLYERGLAPVIVVTGGRQDGD
                     RFTEATTGYNYLRDHGVPDAAIRKEVQGHTTYESVAAAARFLRDEGIDDVILVSSPAT
                     AKRLAGISGDVGLDAAISPSSGSPQIRSLARETVAVSLGRLIGYRRLDRFDT"
     gene            819..1409
                     /locus_tag="VNQ33_01205"
     CDS             819..1409
                     /locus_tag="VNQ33_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96743.1"
                     /translation="MTTSPEQAVRRYLAWIEDPDSAVDQDAVDAAEAAFAAATDPIAK
                     LHAAAARERAAAADTSAIEHDFVVHAKAYADAEQIPASAFEALGVGADVLKQAGFAVA
                     APRGRRRSPSSPAPIRSSGAPRSPHVSVAAIKAVAVQMPKQFTLGQLADKAGGGSPVT
                     VRKAVDELIAEGRAAKLGPAPDHQGPGRAPTLYELR"
     gene            complement(1417..1791)
                     /gene="mscL"
                     /locus_tag="VNQ33_01210"
     CDS             complement(1417..1791)
                     /gene="mscL"
                     /locus_tag="VNQ33_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWJ96744.1"
                     /translation="MLEGFKKFILKGNVVDLAVGIVVGAAFASVITQFTASFITPLIA
                     VFTGGGIDGGTFTINKQVFDYAAFFSAVIGFVITAAVLYFFVIVPMNALNDRRSKGEE
                     SEVEPTNEERMVALLEQIASKP"
     gene            complement(1921..2883)
                     /locus_tag="VNQ33_01215"
     CDS             complement(1921..2883)
                     /locus_tag="VNQ33_01215"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWJ96745.1"
                     /translation="MSGRYAFSMPEPRQRDGWFRIGSLDMTTTNIVVGLGLLSMVLYA
                     IDLEVFYQGAYFAPFVRQGELWRLATWPLVNAPDIWVLIGLVFFWWVGHQVEDQIGRK
                     AQAVLLAAMTVLPAIIVTVIDASRAPNSVEALQSTAYAASVSLLSLGLLCVFALDRPS
                     APFFFGIPAWLIAAVFVAIEALQLIGIRAWSQLLLGALVILIACFGAAQRGMLGEFDW
                     IPRLKALSGGPVSPYGEVGSSRPTSTKRFGKGRSRGKPQAGGGSGGSTVVSGPWNMPT
                     GGPTPLEQAELDVLLDRISAGGIDSLTPHEKERLNALSKRMRES"
BASE COUNT          489 a         1169 c         1153 g          463 t
ORIGIN      
        1 aggcccaggg cccggtgcgg gtcgccgtca gcggtcggtc cgtcggcccg cccctgtggc
       61 agtcgctggc ggtcctcggt cgcgagcgca cgctcgcccg gttgcacgcg atgcaggcca
      121 agctgccctg accgtggctt cctccgacca ggacgagccg gcggtgccga aggctcgctg
      181 gctccggcgc accctctgga cagcggtcat cctgctgggt gtcgtcgtgc tgtacctcgg
      241 ggtcaccttc gtccaggtgt ggcaagccag ccgcagcgac ggtgcccgga aggccgacgc
      301 catcgtcgtg ctcggcgccg cccagtacaa cgggacgccc tcgccggcgc tgcagaaccg
      361 gctcgatcac gcgctcgatc tgtacgagcg ggggctggca ccggtgatcg tggtgaccgg
      421 cggacgccag gacggggacc gcttcaccga ggccacgacc ggttacaact acctccgcga
      481 tcacggtgtt cccgacgcgg cgatccgcaa ggaggtgcag ggtcacacca cctacgagtc
      541 cgtcgccgcc gccgcccggt tcctgcgcga cgaaggcatc gacgacgtca tcttggtctc
      601 gagcccggcg acagcgaagc gcttggccgg catctccggc gacgtcggcc tcgatgccgc
      661 catctcgccg tcgtctggct ccccgcagat ccgctccttg gcacgggaga cggtggcggt
      721 gtcgctgggc cgcctcatcg gctaccgccg cctcgatcgc ttcgacacct gaggccggtc
      781 ctgcgccctg tgcccggggc cgtcggtagc atgcgccggt gaccacgtct cccgagcaag
      841 ccgtccgccg ctacctcgcc tggatcgagg accccgactc tgccgtcgac caggatgcgg
      901 tcgatgcggc cgaggccgcg ttcgccgccg ccaccgaccc catcgccaag ctccacgccg
      961 ctgcggctcg cgagcgggcg gccgccgccg acacatcggc catcgagcac gacttcgtgg
     1021 tgcacgccaa ggcctacgcc gatgccgagc agatcccggc ctcggcgttc gaggcgctgg
     1081 gtgtgggcgc cgatgtgctg aagcaggcgg ggttcgcggt ggccgcgccc cgtggccggc
     1141 gtcggtcgcc ctcgtcgcca gcgcccatcc ggtcgtcggg cgcgccccgg tcgccgcacg
     1201 tgtcggtggc cgccatcaag gcggtggccg tccagatgcc caagcagttc acgctcggcc
     1261 agctcgccga caaggcgggc ggcgggtcgc ccgtgaccgt gcgcaaggcc gtcgacgagc
     1321 tgatcgccga aggccgggcg gccaagctcg gcccggcccc cgatcaccag gggccaggcc
     1381 gagcaccgac gctctacgag ctccgctgag ccgggctcag ggcttcgagg cgatctgctc
     1441 cagcagcgcc accatccgct cctcgttggt gggctcgacc tcggactcct cgcccttcga
     1501 gcgccggtcg ttgagagcgt tcatgggcac gatcacgaag aagtagagga cggccgccgt
     1561 gatcacgaac ccgatcacgg cgctgaagaa ggccgcgtag tcgaacacct gcttgttgat
     1621 ggtgaacgtg ccgccatcga tgccgccgcc ggtgaacacg gcgatcagcg gggtgatgaa
     1681 cgatgcggtg aactgcgtga tgacgctggc gaacgcggca ccgacgacga tgccgacggc
     1741 caggtcgacg acgttgccct tcaggatgaa cttcttgaag ccttcgagca cggggggaac
     1801 ctccagctag ggaatgccgc cgtacgacgg cccccgcatc atggcgcgat ccaccggcgc
     1861 aggtgaccga tctccagcca tgcgtcgggg tcctggatgg agggcaccgc agtggggtgc
     1921 tcaggactcc cgcatgcgct tgctcagcgc gttgagacgt tccttctcgt ggggcgtgag
     1981 cgagtcgatg cccccggcgc tgatgcggtc gagcagcacg tcgagctcgg cctgctcgag
     2041 cggggtcggc ccgcccgtgg gcatgttcca cgggcccgac acgacggtgc tcccgccact
     2101 gccgccaccg gcctgcggct tgccccggct ccggcccttg ccgaagcgct tcgtggacgt
     2161 gggtcggctc gatccgacct ccccgtaggg cgagaccggc ccgccgctga gcgccttgag
     2221 ccgcgggatc cagtcgaact cgccgagcat cccgcgctgg gccgctccga agcaggcgat
     2281 gaggatgacc agcgccccga gcagcagctg ggaccacgcc cggatgccga tcagctggag
     2341 cgcttcgatg gcgacgaaca cggcggcgat caaccacgcg gggatgccga agaagaacgg
     2401 agcgctcggc cggtcgaggg cgaacacgca caggaggccg agggagagca acgacaccga
     2461 tgccgcgtac gccgtcgact ggagggcttc gaccgagttc ggcgcccggg aagcgtcgat
     2521 gacggtgacg atgatcgccg gcagcacggt catcgctgcg agcaggacgg cctgggcctt
     2581 gcgcccgatc tggtcctcga cctggtggcc gacccaccag aagaacacca gaccgatcag
     2641 gacccagatg tcgggcgcgt tgacgagggg ccaggtggcc agcctccaga gctcgccctg
     2701 acgcacgaag ggggcgaagt aggcaccctg gtagaacacc tcgaggtcga tggcgtagag
     2761 gaccatcgac agcaggccga gaccgaccac gatgttcgtc gtcgtcatgt ccaggctccc
     2821 gatgcggaac cacccgtcgc gctggcgggg ctcgggcatg gagaacgcgt accggccgga
     2881 catgggcctg aggttacagg gggaccgcgt cggccctcgt cggcagcgac gatgacccgc
     2941 cggcatccag ggacatcggc ccaggctccc tccgccgtgc gtgggggatt cgaccgggcg
     3001 ctcgttcccg gccagtggcc cggcttcggc ggccgcgctc gcggccgctc gcgggtcggt
     3061 gctcgtgccc gctgcgacgt gggcgggcgt cgcctccctg caccgggacc gtgcccgttg
     3121 ccgaacaggc ccgagggcat cgacacgagt tcaccggctg agggctcccg aggacgtcct
     3181 ctgccggccg ggggaacgac gaacgccctg cctgcgcagg gcgttcgctc gtggcacccc
     3241 caacgggatt cgaacccgtg ccgccacctt gaga
//
LOCUS       DATMFS010000065         5507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_95745, whole genome shotgun sequence.
ACCESSION   DATMFS010000065 DATMFS010000000
VERSION     DATMFS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95745"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VNQ33_01220"
     CDS             <1..555
                     /locus_tag="VNQ33_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWJ96746.1"
                     /translation="SWLAVAMAVQALQAKQVQRIILTRPAVEAGERLGFLPGDLMQKI
                     DPYLRPLYDALHDMVDAEAAARMLERGTVEVAPLAFMRGRTLNNSFIILDEAQNTTPE
                     QMKMFLTRIGFGSKAVITGDSTQVDVAGGRSGLIHMERILGDIDGLAFVRLSGRDVVR
                     HRIVADIVDAYERDAVTNPGPVQP"
     gene            552..1109
                     /gene="ybeY"
                     /locus_tag="VNQ33_01225"
     CDS             552..1109
                     /gene="ybeY"
                     /locus_tag="VNQ33_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWJ96747.1"
                     /translation="MIRPAPPRSGRRRTRGGEGETLVFVADEQSAQPVEVERWEGLAR
                     NVLQEEGVSGDCELALLFVDEPSIAELNQRFMGHEGPTDVLAFPIDDDDVVIGRSPDG
                     SSSGPDRGPVDDVPLLLGDVVVCPAVAARNAPSHAGTYDDEIALLVVHGILHVLGHDH
                     AEPHETEVMQSRERVLLEKFHRKAP"
     gene            1201..2565
                     /locus_tag="VNQ33_01230"
     CDS             1201..2565
                     /locus_tag="VNQ33_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWJ96748.1"
                     /translation="MSGAILAVELTTADIAMAVAIAVLILISVLTAVAETALVRISRA
                     KATALADEGRPGGAALAYLVDQPERWLNALLLVVLICQIVQASLTGVLAGNLFGGWGV
                     IIATGLNVSVMYVLAETAPKTWAIQHTERAALGAARPVKWLADFAPLRGLAKFFIVIT
                     NVILPGKGLKEGPYVSEEELLAVAAEAHESAAIDQDERNLIESIIEFGDTVVREVMVP
                     RPDMITVNHDFRVADVMEVVILNGYSRIPVLGEGIDDVIGLVFAKDLMRAERDEREDE
                     PVANLVRPAKAVPETKPVAELLREMQADQFHMAVVIDEHGGTAGVVTLEDLIEELVGE
                     IVDEFDSEDDRIEHIPSGGVRIKGSLSIDDANDQLPVDLPEGDYDTVSGLVLAELGRM
                     AVAGDAVEVPGTRLTVERVQGRRIMRVRIEQVEKAAADDGDSSNGKTADRGEKADSGD
                     RSAS"
     gene            2562..3404
                     /gene="era"
                     /locus_tag="VNQ33_01235"
     CDS             2562..3404
                     /gene="era"
                     /locus_tag="VNQ33_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWJ96749.1"
                     /translation="MKSGFVTLVGRPNVGKSTLLNHILGQKVTIVSDKPQTTRTQVRG
                     VLTRPDAQVVFVDTPGIHKPRTLLGERLNDAADAAIGDVDVVCLLVDATMPIGPGDKW
                     IAQRVPANAIVVVNKTDKADPTQVLTQLHAAAEQLAGGNEYFPISARTGEGVPALIEA
                     LLARLPEGPQYYPDDMVTDVPEAFWVAELVREQLLAVTRDEVPHSVAARVTEWEWPRI
                     RVEILVERDSQKGIVIGKKGSVLKQVGTNVRKQLPPGAYLELFVKVDKDWQRKSRALE
                     RLGY"
     gene            complement(3505..5397)
                     /locus_tag="VNQ33_01240"
     CDS             complement(3505..5397)
                     /locus_tag="VNQ33_01240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWJ96750.1"
                     /translation="MAASNRSVAALAKLAEAVPGGERRAPQEVMATEVADAISKQRHL
                     LVQAGTGTGKSWAYLVPAILAGQRTVVATATKALQDQLAGKDLPFLQEHLDRPFEFAV
                     LKGRSNYVCVQRIAEISGAGQLALDGLADRASPSELSALKLWAATTPTGDRAELSAEP
                     SPQAWAAVSVSAQECPGARKCPSGDACFAEAARRKAGEADVTVVNLHLLGLDLATDGA
                     ILPDHDLVVVDEAHQLEDIVSDTCGFELSAGRFQNLARSVRAILDDPTTVDEVESSAT
                     TLTTALSEHVGRRLRGEPHPDIQDALATIRARLDRTATALRKIPDGSSDDVKTRKERA
                     VQLTGHLQADVSLAGSVPEGSVAWVEGPDSNPRLRVAPLDVSELLRASLWDAGPVVLT
                     SATLPPGMSERLGLTGDDHKAIDVGSPFDYEDHGLLYCATHIPDPRDGGYEAAVHQEL
                     RALITAAGGRTLGLFTSYRAMDAAVAALKDDLPGPLLSQRDMPKPALVAAFAEDPTAS
                     LFATMGFWQGIDVPGPTLSLVTIDKLPFPRPDEPLLQARREQAKAEAFRTIDLPRTAT
                     MLAQGAGRLIRSQTDRGVVAVLDSRLAKARSYRWILLDALPPFPRTKDRAEVEAFLKA
                     IDADAG"
BASE COUNT          812 a         1979 c         1972 g          744 t
ORIGIN      
        1 agctggctgg ccgtggcgat ggccgtgcag gcgctgcagg ccaagcaggt gcagcgcatc
       61 atcctcaccc gcccggcggt cgaggccgga gagcgcctcg gcttcctgcc cggcgacctg
      121 atgcagaaga tcgacccgta cctgcgcccc ctctacgacg ccctgcacga catggtcgac
      181 gcggaggccg ccgcccgcat gctcgagcgg ggcaccgtcg aggtcgcacc gctcgccttc
      241 atgcgcggcc gcacgctcaa caacagcttc atcatcctcg acgaggcgca gaacaccacg
      301 cctgagcaga tgaagatgtt cctgacccgc atcggcttcg ggtccaaggc cgtgatcacc
      361 ggcgacagca cccaggtcga cgtcgccggc gggcgttcgg gcctcatcca catggagcgg
      421 atcctgggcg acatcgacgg gctcgccttc gtgcgcctga gcgggcgcga cgtcgtgcgc
      481 caccgcatcg tcgccgacat cgtcgacgcc tacgagcgcg acgcggtcac caacccgggt
      541 ccggtgcagc cgtgatccgg ccagcaccgc ccaggagcgg ccgccgtcgc acgcgcggcg
      601 gcgagggcga gaccctggtg ttcgtggccg acgagcagtc ggcccagccc gtcgaggtcg
      661 agcggtggga ggggctcgcc cgcaacgtgc tccaagaaga gggcgtctcg ggcgactgcg
      721 agctggcact gctcttcgtc gacgagccgt ccatcgccga gctcaaccag cggttcatgg
      781 gccacgaggg gcccaccgac gtgctggcct tcccgatcga cgacgacgac gtcgtgatcg
      841 ggcgctcgcc cgacgggtcg tccagcggcc ccgaccgcgg gccggtcgac gacgtcccgc
      901 tgctgctggg cgacgtcgtg gtgtgccccg cggtcgcggc gcgcaacgcc ccgtcgcacg
      961 ccggcaccta cgacgatgag atcgccctgc tggtcgtgca tggcatcctt catgtcctcg
     1021 gccacgacca cgccgagccc cacgagaccg aggtcatgca gtcgcgagag cgcgtgctgc
     1081 tcgagaagtt ccacaggaag gcaccgtgaa cgctcccgac gacgaccccg gcagtcccga
     1141 ggccccgcac gcccgcgcgt ggggggccgt ctctgaccgc ccgagcccgg gagggcgtcg
     1201 gtgagcggcg cgatcctcgc cgtcgagctc acgacagccg acatcgccat ggccgtcgcc
     1261 atcgcggtgc tcatcctcat ctcggtcctc accgccgtcg ccgagaccgc gctcgtgcgg
     1321 atcagccggg ccaaggccac ggccctcgcc gacgaaggcc ggcccggcgg cgcggcgctg
     1381 gcctatctcg tcgaccagcc ggagcggtgg ctcaacgccc tgctgctggt ggtgctcatc
     1441 tgccagatcg tgcaggcgtc gctcaccggt gtgctcgccg gcaacctctt cgggggctgg
     1501 ggcgtcatca tcgccaccgg cctcaacgtg tcggtcatgt acgtcctggc cgagacggcg
     1561 cccaagacct gggccatcca gcacaccgag cgggcagcgc tcggcgcggc ccggccggtg
     1621 aagtggctgg ccgacttcgc cccgctgcgc ggcctggcca agttcttcat cgtgatcacc
     1681 aacgtgatcc tgcccggcaa ggggctgaag gaagggccct acgtgtcgga ggaggagctg
     1741 ctggccgtgg ccgccgaggc ccacgagtcc gccgccatcg accaggacga gcgcaacctc
     1801 atcgagtcca tcatcgagtt cggcgacacc gtcgtgcgcg aggtcatggt gccccggccc
     1861 gacatgatca cggtcaacca cgacttccgc gtcgccgatg tgatggaggt cgtgatcctc
     1921 aacggctaca gccggatccc cgtgctgggc gagggcatcg acgacgtcat cggcctcgtg
     1981 ttcgccaagg acctgatgcg cgccgagcgc gacgagcgcg aggacgagcc ggtggccaac
     2041 ctcgtccgcc cggccaaggc cgtgccggag accaaaccgg tggccgagct gctgcgggag
     2101 atgcaggccg accagttcca catggccgtg gtcatcgacg agcacggcgg caccgccggc
     2161 gtggtcaccc tcgaggacct gatcgaggag ctggtgggcg agatcgtcga cgagttcgac
     2221 tccgaggacg accgcatcga gcacatcccc tcgggcggcg tccgcatcaa gggcagcctc
     2281 agcatcgacg acgccaacga ccagctgccg gtcgacctcc ccgaggggga ctacgacacg
     2341 gtgagcggcc tggtgctcgc cgagctgggc cgcatggccg tggcgggcga tgccgtcgag
     2401 gtccccggca cccggctcac cgtcgagcgc gtccagggcc gccggatcat gcgcgtgcgc
     2461 atcgagcagg tcgagaaggc agcggccgac gacggagaca gctcgaacgg caagaccgcc
     2521 gatcgtggcg agaaggccga ctcgggcgat cggagcgcat cgtgaagtcc gggttcgtca
     2581 cgctcgtggg ccgccccaac gtgggcaaga gcacgctgct caaccacatc ctggggcaga
     2641 aggtcaccat cgtctcggac aagccgcaga ccacccgcac gcaggtccgc ggcgtgctca
     2701 cccggcccga cgcccaggtg gtgttcgtcg acacgccggg catccacaag ccccggaccc
     2761 tgctcggcga gcgcctcaac gacgccgccg acgccgccat cggcgacgtc gacgtggtct
     2821 gcctcctcgt cgacgccacc atgcccatcg gccccggcga caagtggatc gcccagcggg
     2881 tcccggccaa cgccatcgtc gtggtgaaca agaccgacaa ggccgatccc acgcaggtgc
     2941 tcacccagct ccacgccgca gccgagcagc tggcgggcgg caacgagtac ttcccgatct
     3001 ccgctcggac gggggagggg gtgccggcgc tgatcgaggc gctcctcgcc cgcctgcccg
     3061 agggaccgca gtactacccc gacgacatgg tcaccgacgt gcccgaggcg ttctgggtgg
     3121 ccgagctggt gcgcgagcag ctgctcgccg tcacccgcga cgaggtgccc cactcggtcg
     3181 ccgcccgcgt caccgagtgg gagtggcccc gcatccgcgt cgagatcctc gtcgagcggg
     3241 actcccagaa gggcatcgtc atcgggaaga aggggagcgt cctcaagcag gtcggcacca
     3301 acgtgcgcaa gcagctcccg cccggcgcct acctcgagct cttcgtcaag gtcgacaagg
     3361 actggcagcg caagtcccga gccctcgagc gcctcggcta ctgacgcacg ggccgccggc
     3421 cccggttggg tcgctcccac ggagccggga cggggatgag catcacccca ccccatcggc
     3481 acggttcgac ggggacgtga agggtcagcc ggcgtcggcg tcgatggcct tgaggaaggc
     3541 ttcgacctcg gcccggtcct tggtcctcgg gaacgggggg agggcgtcga ggaggatcca
     3601 gcggtagctc ctggccttgg ccaggcggga gtcgagcacg gcgacgacgc cgcggtcggt
     3661 ctgcgagcgg atgaggcggc cggcgccctg ggcgagcatc gtcgccgtcc ggggcaggtc
     3721 gatggtgcgg aacgcctcgg ccttggcctg ctcccggcgg gcctgcagca gcggctcgtc
     3781 gggccggggg aacgggagct tgtcgatggt gacgagtgag agcgtgggcc ccggcacgtc
     3841 gatgccctgc cagaagccca tcgtggcgaa gaggctggcg gtcgggtcct cggcgaaggc
     3901 ggcgacgagg gcgggcttgg gcatgtcccg ttggctgagc agcgggccgg ggagatcgtc
     3961 cttcagcgcc gcgaccgctg cgtccatggc ccggtagctg gtgaagaggc cgagcgtgcg
     4021 gccacccgcg gcggtgatga gggcgcgcag ctcctggtgg accgccgcct cgtacccgcc
     4081 gtcgcgcggg tcggggatgt gggtggcgca gtacaggagc ccgtggtcct cgtagtcgaa
     4141 cggactgccg acgtcgatgg ccttgtggtc gtcgccggtg agcccgagcc gctcggacat
     4201 gcccggtgga agggtcgcgc tggtgagcac gaccggaccg gcgtcccaca ggctggctcg
     4261 gagcagctcg gagacgtcga gcggtgccac ccgcaggcgc gggttgctgt cggggccctc
     4321 gacccaggcc accgaaccct cggggacgct ccccgcgagc gacacgtcgg cctggaggtg
     4381 gccggtgagc tggacggctc gctccttccg ggtcttcacg tcatcggagg agccgtcagg
     4441 gatcttgcgc agcgcggtgg cggtgcggtc gagccgggcg cggatcgtgg ccagggcatc
     4501 ttggatgtcg gggtggggct cgcccctgag gcgacggccg acgtgctcgg agagcgccgt
     4561 ggtgagggtg gtggcgctcg actccacctc gtcgacggtc gtcgggtcgt cgaggatcgc
     4621 ccggacggag cgggccaggt tctggaagcg gcccgctgac agctcgaacc cgcaggtgtc
     4681 cgacacgatg tcctcgagct ggtgggcctc gtcgaccacg accaggtcgt ggtcggggag
     4741 gatcgccccg tcggtcgcga ggtccagccc gagcaggtgg aggttcacca cggtcacgtc
     4801 ggcctcgccg gccttgcgcc gggcggcctc ggcgaagcag gcgtcgcccg acgggcactt
     4861 gcgggcgccg gggcactcct gagccgacac gctcacggcg gcccaggcct gaggcgacgg
     4921 ctcggccgag agctcggccc ggtcgccggt gggcgtggtg gccgcccaca gcttgagcgc
     4981 gctcagctcc gagggcgagg cccggtcggc gaggccgtcg agggcgagct ggccggcgcc
     5041 ggagatctcg gcgatgcgct ggacgcacac gtagttggaa cggcccttga gcacggcgaa
     5101 ctcgaacggc cggtccaggt gctcctggag gaacggcagg tccttgccgg cgagctggtc
     5161 ctggagcgcc ttggtggcgg tggccaccac cgtccgctgg ccggcgagga tcgccggcac
     5221 gaggtacgcc cagctcttgc cggtgcccgt cccggcctgg acgagcaggt gccgctgctt
     5281 cgagatcgcg tcggccacct cggtggccat gacctcctgc ggcgctcgcc gctcgccgcc
     5341 cggaaccgcc tccgccagct tcgccagcgc ggcgacggag cggttcgaag cagccaccga
     5401 ccgaacctac ccaccccgct ccgccacccg cgccatccgc gccatccgcc ccacgatccc
     5461 ctccacccgc gccatccacc ccacgattcc cccctcccat ccacccc
//
LOCUS       DATMFS010000066         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_72259, whole genome shotgun sequence.
ACCESSION   DATMFS010000066 DATMFS010000000
VERSION     DATMFS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72259"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VNQ33_01245"
     CDS             <1..919
                     /locus_tag="VNQ33_01245"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWJ96751.1"
                     /translation="MPITPTEKIWMNGELVAWDDARIHVLTHSLHYGMGVFEGVRAYE
                     TAQGPAVFRLTDHMVRLHDSAKIMMMDLPYSVDELVEATKLVVRESGLPSCYIRPIAY
                     YGYGEMGLNTIPCTVDVAIACWPWGAYLGDDALTKGVRLKTSSWQRHDHNTMPPASKT
                     TGNYVNSSLAKVEALKAGYDEALMLSPGGHIAECTGENVFVARKGRFLTPPLAAGALE
                     GITQDSVATIAKDLGIGVELSSIARSDVYIAEEMFLCGTAAEVSAVNSVDDRKIPCPG
                     PMTTAIAAEYHKAVRGETDRYKDWVEHVS"
     gene            909..2549
                     /gene="cimA"
                     /locus_tag="VNQ33_01250"
     CDS             909..2549
                     /gene="cimA"
                     /locus_tag="VNQ33_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWJ96752.1"
                     /translation="MSPDRSQPLVDEVVEDLPARDPGLPEAVEIYDTTLRDGAQLEGI
                     SLTVDDKLRIADELDELGVHFIEGGWPGANPKDIEFFDRAGRELRFERSLLVAFGSTR
                     RPRGKVDDDPTLRNLLAANTSAVCIVGKSSDFHVTDTLKTTLDEGEAMVADSVEFLTH
                     AGVRVLFDAEHFFDGWKHNPEFSLRVLEAAAEQGAETLVLCDTNGGSLPHEVEAIVRE
                     ITGHFRDDVKIGVHLHDDTGCGVANALAAVRGGAVQVQGTMNGYGERTGNCNLTTIIP
                     NLSLKMGVRTLPEGRLARLTSASNRIAEIVNFTPDPQAPYVGSSAFAHKAGLHVSAIA
                     RRKDAYEHVDPEAVGNDTRFVVSEMAGKATVDLKAKEIGIDLDGPALTSVIDELKRLE
                     HEGYHFEAADGSLELLMRRASGWVQPFFELESYRVIVAEGEDLRHDTEAIVKLKVGDE
                     RIGRIGEGNGPVNALDDAVRAAIGSRYPQLAKVHLTDFKVRVLDTAKGTGAVTRVLID
                     STNGRRSWSTIGVSENIIGAAWQALEDSLVFGLLHTEE"
BASE COUNT          455 a         1041 c          869 g          396 t
ORIGIN      
        1 gatgccgatc acccccaccg agaagatctg gatgaacggc gagctcgtcg cgtgggacga
       61 cgctcggatc cacgtcctca cccactcgct gcactacggc atgggcgtgt tcgagggcgt
      121 gcgggcctac gagacggcgc aggggccggc cgtgttccgc ctcaccgacc acatggtccg
      181 cctgcacgac agcgccaaga tcatgatgat ggacctgccg tactcggtcg acgagctggt
      241 cgaggccacc aagctcgtcg tgcgcgagag cgggctgccg tcgtgctaca tccgcccgat
      301 cgcctactac ggctacggcg agatggggct caacaccatc ccgtgcacgg tcgacgtcgc
      361 catcgcctgc tggccgtggg gcgcctacct gggcgacgac gccctcacga agggcgtgcg
      421 gctcaagacc tcgtcgtggc agcgccacga ccacaacacg atgccgcctg cttccaagac
      481 caccggcaac tacgtcaact cgtcgctcgc caaggtcgag gccctcaagg ccggctacga
      541 cgaggccctc atgctcagcc ccggcggcca catcgccgag tgcacgggcg agaacgtgtt
      601 cgtcgcccgc aagggccgct tcctcacccc gccgctcgcc gccggcgcgc tcgagggcat
      661 cacccaggac tcggtggcca ccatcgccaa ggacctcggc atcggcgtcg agctgtcgag
      721 catcgcccgc tccgacgtgt acatcgcgga ggagatgttc ctgtgcggca ccgccgccga
      781 ggtctccgcc gtgaactcgg tcgacgatcg gaagatcccg tgcccgggtc cgatgacgac
      841 ggccatcgcc gcggagtacc acaaggccgt ccgcggcgag accgaccggt acaaggactg
      901 ggtcgaacat gtctcctgac cgttcccagc ccctcgtcga cgaggtcgtc gaggacctgc
      961 ctgcccgcga ccccgggctt cccgaggccg tcgagatcta cgacacgacc ctgcgcgacg
     1021 gcgcccagct cgaggggatc tccctcaccg tcgacgacaa gctgcgcatc gccgacgagc
     1081 tcgacgagct cggcgtccac ttcatcgagg gcggctggcc cggcgccaac ccgaaggaca
     1141 tcgagttctt cgaccgggcc ggacgcgagc tgcgcttcga gcggtcgctc ctggtggcgt
     1201 tcggctccac ccgccgcccc cgcggcaagg tcgacgacga cccgaccctg cggaacctgc
     1261 tcgctgccaa cacctccgcg gtgtgcatcg tcggcaagag ctcggacttc cacgtcaccg
     1321 acaccctcaa gaccaccctc gatgagggcg aggcgatggt cgccgactcg gtcgagttcc
     1381 tcacccacgc cggcgtgcgc gtcctcttcg acgccgagca cttcttcgac ggctggaagc
     1441 acaacccgga gttcagcctc cgcgtgctgg aggccgctgc cgaacagggc gccgagacgc
     1501 tcgtgctgtg cgacaccaac ggcggctcgc tgccccacga ggtcgaggcc atcgtccgcg
     1561 agatcaccgg ccacttccgc gacgacgtga agatcggtgt ccacctccac gacgacaccg
     1621 gctgcggcgt cgccaacgcc ctcgctgcgg tccgcggcgg ggccgtccag gtccagggga
     1681 ccatgaacgg ctacggcgag cggaccggca actgcaacct caccaccatc atccccaacc
     1741 tgtcgctcaa gatgggggtg cgcaccctgc ccgagggccg cctcgcccgg ctcacctcgg
     1801 ccagcaaccg catcgccgag atcgtgaact tcaccccgga tccgcaggcg ccctacgtcg
     1861 gctcgtcggc gttcgcccac aaggccggcc tgcacgtgtc ggccatcgcc cgccgcaagg
     1921 acgcctacga gcacgtcgac cccgaggccg tcggcaacga cacgcgcttc gtggtgtcgg
     1981 agatggccgg caaggccacc gtcgacctca aggccaagga gatcggcatc gacctcgacg
     2041 gtccggcgct cacctcggtg atcgacgagc tcaagcgcct cgagcacgag ggctaccact
     2101 tcgaagcggc cgacggctcg ctcgagctgc tcatgcgccg ggcctctggc tgggtccagc
     2161 cgttcttcga gctggagtcg taccgcgtga tcgtcgccga gggcgaagac ctgcgccacg
     2221 acaccgaggc catcgtgaag ctgaaggtcg gtgacgagcg catcggccgc atcggcgagg
     2281 gcaacggtcc cgtcaacgcg ctcgacgacg ccgtccgggc cgccatcggc tcgcggtacc
     2341 cccagctggc caaggtccac ctcaccgact tcaaggtgcg cgtgctggac acggcgaagg
     2401 gcaccggcgc cgtcacccgc gtcctcatcg actccaccaa cggccgccgc agctggtcca
     2461 ccatcggcgt cagcgagaac atcatcggtg ccgcatggca ggccctcgag gactccctcg
     2521 tcttcggcct cctccacacc gaggagtagc gcctcacgtg cgtccggctg agcatcgggg
     2581 tgttggcgct ccgggccggt cgagcgcccc cgccgatcga gtgatcgaca cgattggctt
     2641 gtgttcgcgc agctcgaccc ttaacttgcc ctgagtccca tctcgctcgc cgaggtggca
     2701 tgccaaccgg gggagccgtt cgatgccgac gtcaccagcc acgcgcctca agcgccgccg
     2761 c
//
LOCUS       DATMFS010000067         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_281624, whole genome shotgun sequence.
ACCESSION   DATMFS010000067 DATMFS010000000
VERSION     DATMFS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281624"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VNQ33_01255"
     CDS             <1..789
                     /locus_tag="VNQ33_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWJ96753.1"
                     /translation="REAHFLKHLTKVKDRWLEAMDQQADLSRTDEPVHPQLFAKAVND
                     RAADDAFFAVDVGECTVWAARHLRLPGGRRMAGSFNHGSLGAGFPIAMGASALDPSRQ
                     VWAFVGDGGFGMAMQDFVTAVRFDWPVKVLVFDNSELGFVKMETEVSGLPLYPEATGL
                     VNPDFAAYARACGAEGVRVSKAADIIPAVEAALAYDGPFIIDAVVSPGELTMPPHVSM
                     EEAWGFGVSKAKEGLIGIRGEHEQWEGWVDELKANLHTFSLKSI"
     gene            849..1256
                     /locus_tag="VNQ33_01260"
     CDS             849..1256
                     /locus_tag="VNQ33_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96754.1"
                     /translation="MRDGPPMARRVTAALLAAGLALVALGGCGSDEGTRAERAKAEQL
                     QSKLDEHGLDIDIDTAVSLYGDDGGKVCAVAKDPAAMEREGLLAHPRFALRRLHVTKD
                     AAAYTEAVITVYCPDQLGNVRDYIDGLRVADEN"
     gene            1246..2781
                     /locus_tag="VNQ33_01265"
     CDS             1246..2781
                     /locus_tag="VNQ33_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWJ96755.1"
                     /translation="MRTDGADGQDPGGTGGDAALEAFDTEEQAEMGEEVEGTSPSKLG
                     WVTMGFMAMAATGSIAQLSASAEYGLGAVTLYLLPALFFLAPVALVASELGTTWDGGV
                     FVWVREGIGERAGFQAIWLVFVESIALYPSLLSFAAASLAFAIGKPGLASNGLYTGAV
                     ILIVFWGATLIAMRGMGATAKIGRSGLIAGTIIPAASLMLLAVVWLATDHTSATPLEL
                     HNVVPPFTGISSIVLIVSNFIAFAGLEINAVHVREMAQPRRFPRSLAMAAPLIVVMYI
                     LGSIAISVVVPNQALDLNAGAAQAFTILGDGLGISWIGQLLAGLLVFGALAAAIAWVA
                     GPSRGLFIVGRQGLLPPRLQGQNAAGAQVPIFILQGCVVTALSLAFVLLPSVASAFWV
                     LQAMTVILYLSMYIIMFIAAVRLRKTRPTVVRGFRAPAILLLGTVGIIAALSAMAIAF
                     VPPAQLGNHTNSATYAAMLLVGVLVMALPAQFIYTFRKRSWVQEDNVVAPIEMELNGR
                     TET"
     gene            2942..3856
                     /locus_tag="VNQ33_01270"
     CDS             2942..3856
                     /locus_tag="VNQ33_01270"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWJ96756.1"
                     /translation="MEIDLAPLAEHAAAHHGLFRATDAGDRRISREQIRSLLRRGWCA
                     QIVPGVYRVVAAPRTGRQALLAEIWSHPVGTIGSHRGAGFLWWLVGYRSPSVEVTFQL
                     GHNQRNGRRTHASLWLPASHVTVHDSIPVTTVARTLFDLAGLEPRGRVAIALDDALSR
                     KLCTLRQINQVFFALAGRGRRGTVAMREMLEKRGEDYVPPSSALERLARKVFEESGLE
                     MPTFELNLGDDDWIGRVDCVWRDAKLIVELDSERYHGSKSARDADRKRDNRLMATGWR
                     VLRITWDDLKLRPQETVAQIKAALRAAR"
BASE COUNT          576 a         1439 c         1254 g          610 t
ORIGIN      
        1 cgagaagccc acttcctcaa gcacctcacc aaggtcaagg accgctggct cgaggcgatg
       61 gatcaacagg ccgacctcag ccgcaccgac gagcccgtcc acccgcagct gttcgccaag
      121 gccgtcaacg accgggccgc cgacgacgcc ttcttcgccg tcgacgtcgg cgagtgcacc
      181 gtgtgggcgg cacgccacct gcgcctgccc ggcgggcgcc gcatggccgg gtcgttcaac
      241 cacggttcgc tcggcgccgg gttccccatc gccatgggcg cgtcggcgct cgacccgagc
      301 cgccaggtgt gggcgttcgt cggcgacggc ggcttcggca tggcgatgca ggacttcgtc
      361 actgcggtcc gcttcgactg gccggtcaag gtcctcgtgt tcgacaacag cgagctgggc
      421 ttcgtgaaga tggagaccga ggtctccggc cttccgctct accccgaggc caccggcctc
      481 gtgaaccccg acttcgccgc ctacgcccgc gcctgcggcg ccgaaggcgt gcgggtgtcc
      541 aaggccgccg acatcatccc cgcggtcgaa gcggccctcg cctacgacgg gccgttcatc
      601 atcgacgccg tcgtctcgcc cggcgagctc accatgccgc cgcacgtctc catggaggag
      661 gcgtggggct tcggcgtgtc gaaggccaag gagggcctca tcggcatccg cggcgagcac
      721 gagcagtggg agggctgggt cgacgagctc aaggccaacc tccacacctt cagcctcaag
      781 tccatctagg cacccgggtc gcccgaccca cccaccacga cagcgagccg acaggcaagg
      841 ggacgacgat gcgcgacggt ccaccgatgg cacgacgggt caccgccgcg ctcctcgccg
      901 cggggttggc gctcgtcgcg ctcggcggct gcggatccga cgagggcacc agggccgagc
      961 gggccaaagc cgagcagctc cagtccaagc tcgacgagca cgggctcgac atcgacatcg
     1021 acaccgccgt atcgctctac ggcgacgacg gcggcaaggt ctgcgccgtc gccaaggacc
     1081 cggccgccat ggagcgcgag ggcctgctcg cccaccctcg cttcgccctg cgccggttgc
     1141 acgtcaccaa agacgcggcg gcctacaccg aggcggtcat caccgtgtac tgccccgacc
     1201 agctcggcaa cgtccgggac tacatcgacg ggctgcgggt cgctgatgag aactgacgga
     1261 gcagacggcc aggacccggg cgggaccggc ggcgacgccg cgctcgaggc cttcgatacg
     1321 gaggaacagg ccgagatggg cgaggaggtc gaaggcacct cgccctcgaa gctcggctgg
     1381 gtgaccatgg gcttcatggc catggcggcc acgggctcga tcgcccagct gtccgcctcg
     1441 gccgagtacg gcctgggcgc ggtcacgctc tacctgctgc ccgcgctctt cttcctggca
     1501 cccgtcgccc tggtggcgtc cgagctcggg accacgtggg acggcggcgt gttcgtgtgg
     1561 gtccgggagg gcatcggcga acgggccggg ttccaagcca tctggctggt gttcgtcgag
     1621 agcatcgccc tgtacccgtc gctgctgtcg ttcgccgccg cgtcgctcgc gttcgcgatc
     1681 ggcaagcccg gcctggcctc caacggcctc tacacgggag cggtgatcct catcgtgttc
     1741 tggggcgcca ccctcatcgc catgcggggc atgggcgcca ccgccaagat cggccgcagc
     1801 ggcctcatcg ccggcacgat catcccggcg gcgtcgctca tgctcctcgc cgtggtgtgg
     1861 ctggccacgg accacacgtc ggccacgccg ctcgagctcc acaacgtcgt cccgccgttc
     1921 accggcatct ccagcatcgt gctgatcgtg tcgaacttca tcgccttcgc cggcctggag
     1981 atcaacgccg tccacgtgcg ggagatggcc cagccccgcc ggttcccccg cagcctcgcc
     2041 atggccgcgc cgctcatcgt cgtcatgtac atcctcggct cgatcgccat ctcggtggtc
     2101 gtgcccaacc aggcgctcga cctgaacgcc ggcgccgccc aggccttcac catcttgggc
     2161 gacggcctcg gcatctcgtg gatcggccag ctcctcgccg gcctgctggt gttcggcgcc
     2221 ctcgccgcgg ccatcgcctg ggtcgccggc ccgagccgcg ggctgttcat cgtcggccgc
     2281 cagggcctgc tcccgccgag gctccagggc cagaacgcgg ccggcgccca ggtcccgatc
     2341 ttcatcctcc agggctgcgt ggtcaccgcc ttgtccctgg ccttcgtcct gctgccgagc
     2401 gtcgcgtccg cgttctgggt gctccaggcg atgaccgtga tcctctacct gagcatgtac
     2461 atcatcatgt tcatcgccgc cgtccgcctc cggaagaccc gtccgaccgt ggtccgcggc
     2521 ttccgggcgc cggcgatcct gctgctcggc accgtcggca tcatcgccgc cctctcggcc
     2581 atggccatcg ccttcgtgcc cccagcccag ctcggcaacc acaccaactc ggccacctac
     2641 gccgccatgc tcctggtggg cgtcctcgtc atggccctcc ccgcccagtt catctacacg
     2701 ttccgcaagc gctcctgggt ccaagaggac aacgtcgtcg ccccgatcga gatggagctc
     2761 aacggccgca ccgaaacctg agcccgcccc accaacccca accccaactg accgaacttt
     2821 tcgtgaagtt ggtggctgag ccaccacgag cacgaaatgt tcggctgggg gttggttacc
     2881 gcagcgccag ttccgtcgca tcgctggcgt gttgcaacga gctgcggaac ggttgcgagc
     2941 cgtggaaatc gacctcgccc ctctcgccga gcacgcagca gcccaccacg gcctgttccg
     3001 agcaaccgat gccggagacc ggcgcatcag ccgtgaacag atccggagcc tcctgcgccg
     3061 cggttggtgc gcccagatcg tgcctggcgt ctaccgcgtc gtggcggcac cgcggacggg
     3121 gcgccaagcc ctgcttgccg agatctggtc gcatcccgtc ggaacgatcg gttcgcaccg
     3181 aggcgccgga ttcctctggt ggctggtcgg ctaccggtct cccagcgtcg aggtcacgtt
     3241 ccagctgggg cacaaccaac ggaacgggcg ccgaacgcac gcctcgctct ggctgccggc
     3301 gtcgcacgtc accgtccacg actcgatccc ggtgacgacg gtcgctcgga cgctcttcga
     3361 tctggctggc ctcgaaccga ggggccgcgt cgcgatcgct ctggatgacg cgctgtcacg
     3421 gaagctgtgc acgctgcggc agatcaacca ggtgttcttc gctctcgccg ggcgagggcg
     3481 gcgagggacc gtcgccatgc gcgagatgct ggagaagcga ggcgaggact acgtccctcc
     3541 ctcctcggcg ctcgagcggc tcgcccgcaa ggtcttcgag gagtctggcc tcgagatgcc
     3601 gacgttcgaa ctgaacctcg gtgatgatga ctggatcggc cgagtcgact gcgtatggcg
     3661 ggacgccaag ctcatcgtcg agctcgactc ggagcgctac cacggctcga agtcggcccg
     3721 agacgcagac cgaaagcgcg acaaccggtt gatggccact ggttggcggg tgctgcggat
     3781 cacctgggac gacctgaagc tccggcccca ggagaccgtc gcccagatca aggccgcgct
     3841 gcgagctgct cggtgaccca tccccagccg aacttttcg
//
LOCUS       DATMFS010000068         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_72318, whole genome shotgun sequence.
ACCESSION   DATMFS010000068 DATMFS010000000
VERSION     DATMFS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72318"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VNQ33_01275"
     CDS             complement(<1..277)
                     /locus_tag="VNQ33_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWJ96757.1"
                     /translation="MPEGHTIHRLARDLRADLGGRALRVTSAQARAEDTAALLDGHTL
                     RRTEAWGKHLFVTFESHQILHVHLGLIGKWFRRAPGGADGTLPDPTDT"
     gene            320..393
                     /locus_tag="VNQ33_01280"
     tRNA            320..393
                     /locus_tag="VNQ33_01280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Leu,seq:tag)
     gene            616..1200
                     /locus_tag="VNQ33_01285"
     CDS             616..1200
                     /locus_tag="VNQ33_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWJ96758.1"
                     /translation="MPVAQGPGGFDPGSDIYNRLLKERIVFLGTDVNDTIANQLAAQI
                     LYLDGQDSEKDIYLYINSPGGSISAGMAIYDTMQFVTADVATVCMGLAASMGQFLLCA
                     GAPGKRSALPHSQIMMHQPSGGIQGQASDIAIQAERMVYIKRLMAEKIAEHSGQSVEQ
                     IEEDSERDRWFTANEAKEYGLIDNVITRRAAIES"
     gene            complement(1222..1782)
                     /locus_tag="VNQ33_01290"
     CDS             complement(1222..1782)
                     /locus_tag="VNQ33_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96759.1"
                     /translation="MHRSPSGAARALTAALLLATAAAGCSKHSGSTAEFCTRVKQVPA
                     LETVLDRFSEADPGALADRIDKARTAYDDLADAAPKQIAEATDQVVGLVDAVLDAVEQ
                     HPTDPAKASAQLRKAVAARKGVEADRAEVAAFAQEHCDVRLDATLTDATAPTTAATTT
                     TTEVDSVITAPPSGPATSTTAATTGG"
     gene            complement(1786..>2502)
                     /gene="rpe"
                     /locus_tag="VNQ33_01295"
     CDS             complement(1786..>2502)
                     /gene="rpe"
                     /locus_tag="VNQ33_01295"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWJ96760.1"
                     /translation="DAEVPSDAPPRPIHIAPSVLPADFAKMGDELRRVADAGADRIHW
                     DVMDGQFVPNMTFGPAMIAACRSAVDLPFEAHLMVNTPEVLLSQYIDAGCQLITIHAE
                     STIHLHRCLSQINEGGSRAAVTIVPSTPVSAIEHVLDLVDMVLIMTVNPGFGGQTYLA
                     SQEAKVAGLRRLIVERGLEIDIEVDGGIAPSTIADATAAGANVFVAGSALFRDPAGLA
                     HAIEDLRTRAQSAQAGVHAA"
BASE COUNT          370 a          815 c          933 g          384 t
ORIGIN      
        1 tggtgtcggt gggatcgggg agggtcccgt ccgcgccacc gggcgcccgg cggaaccact
       61 tgccgatgag cccgaggtgc acgtggagga tctggtgcga ttcgaaggtg acgaagaggt
      121 gcttgcccca tgcctcggtg cgtcggaggg tgtgcccgtc gagcagggcg gcggtgtctt
      181 cggcccgggc ctgcgcgctg gtcacgcgca gcgcgcggcc tccgaggtca gcccggagat
      241 ccctcgccag tcggtggatg gtgtgacctt cgggcatgga ggagcagggt gccagacgac
      301 cggtacgatc ggccacctcg ccggcgtagc ccaattggca gaggcaccag gtttaggtcc
      361 tgggggttgc gagttcgagt cccgccgccg gcacggccaa cggggaagtt ggaggatcgg
      421 tgccggtcac gggcaccttt cctccaactt ctgccaccat ccgccaactt ggtggatcga
      481 ggtcggtatg cgccaacatc ctccaagttg gaggcgaggg agcggtgagg aagcggcgag
      541 gagcggtgac gtgccaggtc gcgggaaggg cttgggcggc cgctagcctg cgccgctatg
      601 gataccccgc tcgtgatgcc cgtcgcccaa ggccctggcg gcttcgaccc cggctcggac
      661 atctacaacc ggctcctcaa ggagcgcatc gtcttcctcg gcaccgatgt caacgacacc
      721 atcgccaacc agctcgcggc gcagatcctg tacctcgacg ggcaggactc cgagaaggac
      781 atctacctct acatcaactc gcccggcggc tccatcagcg ccggcatggc catctacgac
      841 acgatgcagt tcgtgacggc cgatgtcgcc accgtgtgca tgggcctcgc ggccagcatg
      901 ggccagttcc tgctctgcgc cggtgcgccg gggaagcgct cggcgctgcc ccacagccag
      961 atcatgatgc accagccctc gggtggcatc cagggccagg catcggacat cgccatccag
     1021 gcggagcgga tggtctacat caagcgcctc atggccgaga agatcgccga gcactccggc
     1081 cagtcggtgg agcagatcga agaggactcc gagcgcgacc gctggttcac ggcgaacgag
     1141 gccaaggagt acggcctcat cgacaacgtc atcacgcgtc gggcagccat cgagagctga
     1201 ccagcgccac gacccggcgg ctcagccgcc ggtcgtcgca gccgtcgtcg aggtggccgg
     1261 gcctgatggc ggcgccgtga tcacggagtc gacctcggtg gtggtcgtgg tggcggccgt
     1321 ggtcggcgcc gtggcgtcgg tgagggtcgc gtcgagtcgc acgtcgcagt gctcctgggc
     1381 gaacgcggcg acctcggcgc ggtcggcctc gacgcccttg cgcgccgcca cggccttgcg
     1441 cagctgcgcg gatgccttgg cgggatcggt cgggtgctgc tcgacggcgt cgaggacggc
     1501 gtcgacgagg ccgaccacct ggtcggtcgc ttccgcgatc tgcttcggtg cggcgtcggc
     1561 gaggtcgtcg taggcggtgc gggccttgtc gatgcgatcg gcgagcgcac cgggatcggc
     1621 ctcgctgaag cggtcgagca cggtctcgag cgccgggacc tgcttgaccc gggtgcagaa
     1681 ctccgcggtg gacccgctgt gcttgctgca tcccgctgcg gcggtggcga gcagcagcgc
     1741 agcggtgagg gcccgtgcgg ccccgctggg ggagcggtgc atcggtcagg cggcgtggac
     1801 gccggcctgc gcggactggg cccgggtccg caggtcctcg atggcgtggg ccagtccggc
     1861 ggggtcgcgg aacaaggcgc tgccggcgac gaacacgttg gcgccggcgg cggtggcgtc
     1921 ggcgatggtg ctcggggcga tgccgccgtc cacctcgatg tcgatctcga gcccccgctc
     1981 cacgatcagg cggcggagcc cggccacctt ggcctcctgc gaggcgaggt aggtctggcc
     2041 gccgaagccg gggttgaccg tcatgatcag caccatgtcc accaggtcga ggacatgctc
     2101 gatggccgag accggcgtcg agggcacgat ggtcaccgcc gcccgggagc cgccctcgtt
     2161 gatctgcgac aggcaccggt ggaggtggat cgtggactct gcatggatgg tgatgagctg
     2221 gcacccggca tcgatgtact gcgacagcag cacctcgggg gtgttcacca tgaggtgggc
     2281 ttcgaacggc aggtccacgg ccgaccggca ggcggcgatc atcgccggcc cgaacgtcat
     2341 gttggggacg aactggccgt ccatcacgtc ccagtggatg cggtcggcgc ccgcatcggc
     2401 cacccgcctc agctcgtcgc ccatcttcgc gaagtcggcg gggagcacgg acggagcgat
     2461 gtggatcggg cggggaggag catcggaggg cacctcggca tc
//
LOCUS       DATMFS010000069         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_165232, whole genome shotgun sequence.
ACCESSION   DATMFS010000069 DATMFS010000000
VERSION     DATMFS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165232"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..>2267)
                     /locus_tag="VNQ33_01300"
     CDS             complement(251..>2267)
                     /locus_tag="VNQ33_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ96761.1"
                     /translation="VHPDDLDRAIAGWNRSLAEPGVEVSEEVRMRSANGTWQDISITG
                     VNLLHDPEVGGIVVTSRNVTDLRRAQRLAASQASVLELIARSEPLPAVAEACLQLLAD
                     NGVSGRSSIYLIDDDHFELSAGAAPASLVEWMREAPPSLTRSLCDEAVATEMPAIAPD
                     IEQATISDGLRYIAANEGIRAAWSQPILAISTGRPIGSLSTLYDEVHDPSAHERQAAD
                     VSCSLMAIAIERAETEARLAHQALHDGLTGLPNRTLLLDRLDHALARRERNGADIALL
                     FCDIDRFKVINDSLGHNVGDQLLGAFAQRFRSVVAPGDTVARFGGDEFVVLIEDITSP
                     THASQVAQDLADALAQPFLLPGGNEVYLTVSIGLAGATDHRTGDAWLRDADAAMYRAK
                     EAGRNRLAEFDTDMRDAAMVRLQIESDLRRAVDRGELVVHYQPLIDLRNGRICGAEAL
                     VRWQHPERGLLGPADFIAVAEDIGTIHELGRYVLEVAAASMAELAPIGPDRPFQLGVN
                     VSARQLNSGELDRVVQSVLERTGWPGDALLLEITESALLHGLDGPVDVMTRLHDLGVS
                     LAIDDFGTGYSSLARLGHLPVDQVKIDQSFVGAIDREGDRLSNIVDAVLAIADALGHR
                     TTAEGVETSAQLDYLRRGRCDYGQGYLFAKPLPFDEFARLLETDPRW"
BASE COUNT          299 a          781 c          870 g          317 t
ORIGIN      
        1 ctgcggcagg aggtcgatgc cctcgggctt cgacgggtcc ggccgcgtcc ccgggcccag
       61 cacgcccggt gccgcccaca ccgcgggccc gcggaggccc ccggcgagcg ccaggcccgc
      121 ccccgtggcc atcccctgca gcacccgtcg tctcgtcacc cttcgcacca tgggcgcgaa
      181 ggctacgacg gcgacgtggc ccgtgcgtgt gcagcagggg gccgggaggt ggcgggaggg
      241 tgaaccagcg ctaccagcgc ggatcggtct ccaggagccg ggcgaactcg tcgaacggca
      301 gcggcttggc gaagaggtag ccctggccgt agtcgcagcg gccccggcgg aggtagtcga
      361 gctgcgccga ggtctcgacg ccctcggcgg tggtccggtg cccgagggca tcggcgatgg
      421 cgagcacggc gtcgacgatg ttgctcagcc ggtcgccctc ccggtcgatc gccccgacga
      481 acgactggtc gatcttcacc tggtccaccg gcaggtggcc gaggcgggcg agcgacgagt
      541 agccggtgcc gaagtcgtcg atggcgagcg acacgcccag gtcgtgcagc cgggtcatca
      601 cgtcgaccgg cccgtcgagg ccgtggagca gcgcgctctc ggtgatctcg aggagcagcg
      661 cgtcgccggg ccacccggtc cgttcgagca cggactgcac gacgcggtcg agctcacccg
      721 agttgagctg gcgggccgac acgttgacgc cgagctggaa cggccggtcc gggccgatcg
      781 gcgccagctc ggccatcgac gccgccgcca cctcgaggac gtagcggccc agctcgtgga
      841 tcgtcccgat gtcctcggcg acggcgatga agtcggccgg ccccagcagc ccgcgttcgg
      901 ggtgctgcca gcgcacgagc gcctcggcgc cgcagatgcg cccgttgcgg aggtcgatga
      961 ggggctggta gtggacgacg agctcgccgc ggtcgacggc gcggcggagg tcgctctcga
     1021 tctggaggcg gaccatggcg gcgtcgcgca tgtcggtgtc gaactcggcc aggcggttcc
     1081 ggcccgcctc cttcgcccgg tacatggccg catccgcgtc gcgcagccag gcgtcgccgg
     1141 tgcggtgatc ggtcgctccc gccagcccga tgctgacggt caggtagacc tcgttcccgc
     1201 cggggaggag gaacggctgg gccagggcgt cggccaggtc ctgggccacc tgcgaggcat
     1261 gggtcggcga ggtgatgtcc tcgatcagca cgacgaactc gtcgccgccg aagcgggcca
     1321 cggtgtcgcc cggtgccacg acggagcgga agcgctgggc gaacgcgccg agcagctggt
     1381 cgcccacgtt gtggccgagg ctgtcgttga tcaccttgaa ccggtcgatg tcgcagaaca
     1441 gcaaggcgat gtcggcgccg ttgcgctccc ggcgggcgag ggcgtggtcg agccggtcga
     1501 gcagcagcgt gcggttgggc agcccggtca ggccgtcgtg cagcgcctgg tgggccaggc
     1561 gcgcctcggt ctcggcccgc tcgatggcga tggccatgag gctgcagctc acgtcggcgg
     1621 cctggcgctc gtgggcggac gggtcgtgga cctcgtcgta gagcgtggac agcgagccga
     1681 tgggccggcc ggtgctgatg gcgaggatcg gctgcgacca cgccgcccgg atgccttcgt
     1741 tcgcggcgat gtagcgcagc ccgtccgaga tggtcgcctg ctcgatgtcg ggggcgatcg
     1801 ccggcatctc ggtggcgacg gcctcgtcgc acaggctgcg cgtcagcgac gggggtgcct
     1861 cccgcatcca ctccaccagg gaggcaggcg cggcccccgc gctcagctcg aagtggtcgt
     1921 cgtcgatcag gtagatcgac gaccgtccgc tcaccccgtt gtcggccagc agctgcaagc
     1981 acgcctcagc cacggccggc agcggctcgc tccgggcgat gagctcgagg accgaggcct
     2041 ggctcgcggc gagccgctgc gctcggcgca ggtcggtcac gttgcgggag gtgacgacga
     2101 tgccgcccac ctcggggtcg tgcaggaggt tgacaccggt gatcgagatg tcctgccacg
     2161 ttccgttggc ggagcgcatg cgcacctcct cggacacctc gaccccgggt tccgcgagcg
     2221 accggttcca gccggcgatg gcccggtcga ggtcgtcggg gtggacg
//
LOCUS       DATMFS010000070         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_95903, whole genome shotgun sequence.
ACCESSION   DATMFS010000070 DATMFS010000000
VERSION     DATMFS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95903"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VNQ33_01305"
     CDS             complement(<1..589)
                     /locus_tag="VNQ33_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HWJ96762.1"
                     /translation="MKMFSKTPLAVRALGVGAVLFGLAACQPVATVPGNGSIPGNGGA
                     PAPTVAPTTTTAPPTTTTTTTAPASTTTTAVPAPSGWKLVGGDEFNDSTLDTSKWKPY
                     HNNYGAGNLELECNTPSNVAEGGGSLKITAKKQTVACPNAGTYNYTSGFVGSRDVNKY
                     YPRYARFEMRAKLPHAQGLWPAFWLRHRNGAGTAEV"
     gene            complement(768..1628)
                     /gene="larE"
                     /locus_tag="VNQ33_01310"
     CDS             complement(768..1628)
                     /gene="larE"
                     /locus_tag="VNQ33_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWJ96763.1"
                     /translation="MTALGDPPAPSVEIPGGLGTAYERLRAVVADLGSVMVAFSGGAD
                     SALVAWVAHDVVGPGRATAVTAVSASLAGEEHADCRALAAEWGLTWQIVATGELGDAR
                     YVANEGDRCYWCKTALLDAIEPLASAADATVVLGVNLDDLGDHRPGQAAAAERGARFP
                     LVEAGFTKSMVRDLSRHLGLRTADKPAAACLASRIPYGTPVTVGVLAEVEAAESALRA
                     LGFTELRVRHYRELARLEVPVDRLAEVVERRQEVVDAVRSAGYRYVTLDLQGLRSGNL
                     NGALGSASAT"
     gene            complement(1635..2321)
                     /locus_tag="VNQ33_01315"
     CDS             complement(1635..2321)
                     /locus_tag="VNQ33_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWJ96764.1"
                     /translation="MPEALEASVGIGVVHLFCTVGEGTDRAAVETAVKAAGADELQVV
                     PVAVLGHKADVAFMVLGKDWWKLKSFQTAIQVAGLTVIDSYVSMTELSEYAKGLPEQM
                     ANARLYPQLPPEGMSAWCFYPMSKKRERKANWFTLPFEERKELMLEHGASGRKFAGRI
                     VQLITGSAGVDDYEWGVTLFAQRPDDLKEVVYTMRFDEASAKYADFGPFVAGVVGTLG
                     EVLDAAGLPD"
BASE COUNT          360 a          909 c          893 g          384 t
ORIGIN      
        1 cgacctcagc ggtgccggcg ccgttgcggt ggcgcagcca gaaggcgggc cagagccctt
       61 gggcgtgggg cagcttggct cgcatctcga agcgggcgta gcgggggtag tacttgttca
      121 catcgcggga gccgacgaag cccgaggtgt agttgtaggt gccggcgttg gggcaggcga
      181 cggtctgctt cttggcggtg atcttcagcg agccgccacc ctcggccacg ttggaggggg
      241 tgttgcactc gagctcgagg ttccccgctc cgtagttgtt gtggtacggc ttccacttcg
      301 acgtgtcgag ggtcgaatcg ttgaactcgt cgccgccgac gagcttccac ccgctcggcg
      361 cgggcaccgc cgtggtggtg gtcgaggcgg gtgccgtggt cgtcgtggtg gtcgtcggcg
      421 gcgcggtcgt cgtcgtgggg gccaccgtcg gcgcgggggc accgccgttg ccggggatcg
      481 acccgttgcc cggcacggtc gccaccggct gacaggccgc gaggccgaac agcaccgcgc
      541 cgacgccgag cgctcggact gcgagagggg tcttcgagaa catcttcatc gccttaccaa
      601 cttcgggaac gtccagttct gacgtcgttg gttacggcgc ggggcggcgc tcgcttgagc
      661 gctgagagcg actttctcgc cactttgggc gatatttttc gcttgatcag ctggttctca
      721 cggagggcgt ttgaattccc acagagcgta cctggaaggc gggttcctca ggtcgctgaa
      781 gcactgccga gcgcgccgtt gaggttgccc gagcgcagcc cctggaggtc gagggtcaca
      841 tagcggtacc cggcgctgcg caccgcgtcg accacctcct gccgtcgctc gaccacctcg
      901 gcgaggcgat cgacgggcac ctccagccgg gccagttccc ggtagtggcg cactcgcagc
      961 tccgtgaacc cgagggcccg gagggcggac tcggcggcct ccacctcggc gagcacgccc
     1021 acggtcaccg gcgtgccgta ggggatccgg ctggcgaggc acgccgcggc cggcttgtcg
     1081 gcggtgcgca gccccaggtg ccggctcagg tcccgcacca tggacttggt gaagccggcc
     1141 tccacgagcg ggaagcgggc gccgcgctca gccgccgccg cctgcccggg ccggtgatcg
     1201 cccaggtcgt cgaggttcac gcccaacacg acggtggcgt ccgcggcaga agccaacggc
     1261 tcgatcgcgt cgagcagcgc cgtcttgcac cagtagcacc gatcgccctc gttggccacg
     1321 tagcgagcgt cgcccagctc ccccgtggcc acgatctgcc aggtgaggcc ccactcggcc
     1381 gccagcgccc ggcagtcggc gtgctcctcg ccggcgagcg aggccgacac cgccgtgacc
     1441 gccgtggccc ggcccggccc caccacgtcg tgcgccaccc aggcgacgag ggccgagtcg
     1501 gctccgccgg agaacgccac catcaccgaa ccgaggtcgg ccacgaccgc ccgcaaccgc
     1561 tcgtaggcgg taccgagccc gccggggatc tcgacggatg gtgcgggcgg atcgcccagc
     1621 gcagtcaccg tcgatcagtc cgggaggccg gccgcgtcga gcacctcgcc gagcgtgccc
     1681 accaccccgg cgacgaacgg gccgaagtcc gcgtacttgg ccgatgcctc gtcgaagcgc
     1741 atcgtgtaca cgacctcctt gaggtcgtcg ggcctctggg cgaacagggt gacgccccac
     1801 tcgtagtcgt cgacgccagc cgacccggtg atgagctgca cgatgcgccc agcgaacttc
     1861 cgtccgctgg caccgtgctc cagcatcagc tccttgcgct cctcgaacgg cagggtgaac
     1921 cagttcgcct tgcgctcgcg cttcttcgac atcggataga agcaccacgc gctcatgccc
     1981 tcgggcggga gctgcgggta gaggcgggcg ttggccatct gctcgggcag gcccttggcg
     2041 tactcggaca gctcggtcat cgacacgtac gagtcgatga cggtcagccc agcgacctgg
     2101 atcgccgtct ggaaggactt gagcttccac cagtccttgc ccagcaccat gaaggcgaca
     2161 tcggccttgt ggccgagcac ggcgacgggc accacctgga gctcgtcggc cccggccgcc
     2221 ttcacggcgg tctccaccgc ggcccgatcc gtgccctcgc ccaccgtgca gaacaggtgc
     2281 acgacaccga taccgaccga cgcttccagg gcttcaggca tgcaacgagg ctaccgccgg
     2341 gccccgtcca ccaccccagc cgacacccag gcgagccggg tcacgtgcca cggaccggtt
     2401 cggctgagcg ctgccggcgg ggcgcggcgc gtcagccgct ggcggagtcg ctgcggagcg
     2461 ggacgacgtt cgatgcctcg gcgccgtcgc tggccgcggc cgctgctccg tcacggcccg
     2521 agcggagcag gcggcgccag cgcttg
//
LOCUS       DATMFS010000071         5280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_189048, whole genome shotgun sequence.
ACCESSION   DATMFS010000071 DATMFS010000000
VERSION     DATMFS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189048"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VNQ33_01320"
     CDS             <1..862
                     /locus_tag="VNQ33_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96765.1"
                     /translation="SIVASDVVIGDHGSVTFYAAMLGRPVLLGSFGTSELVPGTTTDL
                     LGKVAPRVDGDGPLGEQIDEVLPLAPHVRDPIRDAAFAFQGQALPRHRDLLYRLIDLP
                     VPNRPVAPRLPAVPEARWDEPSAWLVETEICSIDPIRIRVDRFAPGLTARRQPSPRQN
                     HLAVVDGIDAAHLLESAGVIITSPSRLRTNPISGLRSVAARWPGCRVVAAPDGPDRWQ
                     VFTRDGHRTVASWDSSTGLDQVPASVVPSVVYAWSLTERWGPTDAPVTIEIEVADRVT
                     ALSITVARAD"
     gene            complement(866..2959)
                     /locus_tag="VNQ33_01325"
     CDS             complement(866..2959)
                     /locus_tag="VNQ33_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ96766.1"
                     /translation="MEVPLSDGSSPSAGPRNWFSGEAHVSVQARDIGVLSLIVPHPDG
                     LPSPRQLPPSPKQFVNRAVEMAALDDLVEHRGLGPAVAVITGMPGVGKSAIGRYWAHT
                     VGATFDEAHLYANFGDLRVGGGVGVSDVLGGFLRALGLPDAAIPPGLAERTALYRSAT
                     AEMRMLVFLDDVAHDAEVRPLIPSSSGAVVVVTSQGYLEELIVDGAGVVEMLPLSPDH
                     AELLLIEMIGPARVVAGSPDVQALRDHCGGLPLALRMCAAWLVRRPSRTIADLNATLD
                     DRDAEPIVGIFDMAYGELPDDLAGRYRDLGLLPSSVLSPESAGALWGSSERDALRCLE
                     ELAERHLLDVVGDRRYRIHGLVRAHARRRALLEDTSARIETVTRRVVTWYLAATMRAD
                     RAILPDRLRLAAPRGEAMHPPLRFDSAADAFGWLEEERPTFVAVIEEAHRRGWHEDAW
                     AMCEALWPFLFNRKHHVDWVATHRIGIEAAAACEDPYAQARVRMQLARASIELGQYEE
                     AEEELTEAARLAAGHEALSGSIMEFTGILCTESGRHVDAVQWLERSRDLYTRLGGRRA
                     VLLQEFHLGRAHLRAGNPSQAIGSLEAALGAVDTVDDRITASQIMIDLARALLAEGSV
                     RTARDHLEEAVRLARAVPLPFDEATAGVLLGQVLIDQGDGAGARRCWQRAHDLLVAVG
                     SPDADAVRARLAQIG"
     gene            complement(3277..3360)
                     /locus_tag="VNQ33_01330"
     tRNA            complement(3277..3360)
                     /locus_tag="VNQ33_01330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3324..3326),aa:Leu,seq:gag)
     gene            complement(3440..3685)
                     /gene="secG"
                     /locus_tag="VNQ33_01335"
     CDS             complement(3440..3685)
                     /gene="secG"
                     /locus_tag="VNQ33_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWJ96767.1"
                     /translation="MTPFKWFLVGLHVIDSLVLLFLVLLHSGKGGGLSDMFGGGMGAA
                     AQGSTVVEKNLDRATVIAAVCFTFTTLSLGLFLSTPA"
     gene            complement(3970..4755)
                     /gene="tpiA"
                     /locus_tag="VNQ33_01340"
     CDS             complement(3970..4755)
                     /gene="tpiA"
                     /locus_tag="VNQ33_01340"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWJ96768.1"
                     /translation="MAKSTRTPLISGNWKMHHNHFEAIQTVQKLAYLLHKDTYETVDV
                     SVHPPFTDIRSVQTVLEADDIPISIGAQHCYIEEKGAFTGEVSPAMLAKLNVEYVIAG
                     HSERREIFGETDELINKKVHAIFKWGMTPILCCGETDEEREAGGTEEKVAGQVRAGLA
                     GLTAEQVGSMVIAYEPIWAIGTGKTASADDAQETCRLVRSVVAEVAGPDAAASVRIQY
                     GGSVKPANAAELMARPDIDGALVGGASLVAEDFAGIVDFQNHT"
     gene            complement(4903..>5280)
                     /gene="pgk"
                     /locus_tag="VNQ33_01345"
     CDS             complement(4903..>5280)
                     /gene="pgk"
                     /locus_tag="VNQ33_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWJ96769.1"
                     /translation="PSAGGEVRQFGKDLPDGWMGLDIGPGTAAEFSDVIGEARQVLWN
                     GPMGVFEDPRFEAGTRTVAEAVADCRGFTVIGGGDSAAAAAQFGLADQIDHVSTGGGA
                     SLELIEQGDLPGLAALRSAPNAS"
BASE COUNT          832 a         1880 c         1775 g          793 t
ORIGIN      
        1 ctccatcgtc gcctcagatg tggtgatcgg cgaccacgga agcgtgacgt tctacgcggc
       61 catgctcggg cgtcccgtgc tgctcggctc cttcggcacc tcggagctgg tgccgggaac
      121 gaccaccgac ctcctgggca aggtggcgcc acgagtcgac ggcgacggtc ccctgggcga
      181 acagatcgac gaggtgctac cgcttgcacc gcacgttcgc gaccccatcc gtgacgcggc
      241 cttcgcgttc caaggacagg cgctgccccg gcaccgggat ctgctgtacc gcctgatcga
      301 cctccccgtc cccaaccgtc ccgtcgcgcc gcggctgcca gctgttcccg aggcgcgctg
      361 ggacgaaccc tcggcatggc tcgtggagac cgagatctgc tccatcgatc cgatccggat
      421 ccgggtcgac aggttcgcgc ccggcctcac cgcgaggcgc cagccaagtc ctcggcagaa
      481 ccacctcgcc gtcgtcgacg gcatcgatgc tgctcacctg ctcgagagcg cgggcgtgat
      541 catcacctcg ccttcccggc tacgaaccaa cccgatcagc ggacttcggt ccgtcgccgc
      601 acggtggccg ggttgccggg tggtcgccgc gccagacggg ccggaccgct ggcaggtctt
      661 cacccgggac gggcaccgca ccgtggccag ttgggactcg tccaccggcc tcgaccaggt
      721 gccggcttcg gtcgttcctt ccgtggtgta cgcgtggagc ctgaccgagc gctgggggcc
      781 gaccgacgcc ccggtgacca tcgagatcga ggtggccgat cgggtcacgg cgttgagcat
      841 caccgtggcc cgagccgact agcagctacc cgatctgcgc caacctcgct cggaccgcgt
      901 cggcatcggg gctaccgacc gcgaccagca ggtcgtgggc ccgctgccag cagcgccgcg
      961 ccccggcccc atccccctgg tcgatcagga cctgaccgag caggacaccg gcggtcgcct
     1021 cgtcgaaggg cagcgggacc gcccgagcaa gccgaaccgc ctcctcaagg tggtcgcggg
     1081 ccgttcgcac ggagccctcg gcgagcagcg ccctcgcgag gtcgatcatg atctggctgg
     1141 ccgtgatgcg gtcgtccacg gtgtccaccg cgcccagcgc cgcctccagg gagccgatcg
     1201 cctgcgacgg attccctgcc cgcaggtgcg cccgtcccag gtggaactcc tggaggagca
     1261 ccgctcgacg gccgccgagg cgggtgtaga ggtcacgtga gcgctccaac cactggaccg
     1321 catcgacatg gcggccggac tcggtgcaga ggatcccggt gaactccatg atcgaccctg
     1381 agagcgcctc gtggccggcg gcgaggcgcg ctgcttcggt gagctcttct tcggcctcct
     1441 cgtactggcc gagctcgatg gaggcacggg ccagctgcat ccgcacccgc gcttgggcgt
     1501 acgggtcctc acaggcggcc gcagcttcga tcccgatccg gtgggtcgcg acccagtcca
     1561 catggtgctt gcggttgaag aggaacggcc acagcgcctc gcacatcgcc caagcatcct
     1621 cgtgccagcc gcgacggtgg gcctcctcga tgaccgccac gaacgtcggc cgctcctcct
     1681 cgagccaccc gaacgcgtcc gccgcgctgt cgaagcgcaa cggcggatgc atcgcctccc
     1741 ctctcggcgc tgcgaggcgg aggcggtccg gcaggatcgc ccggtctgct cgcatcgtgg
     1801 cggccaggta ccacgtgacc actcgacgcg tcaccgtctc gatccgagcg ctggtgtctt
     1861 cgaggagcgc acgccggcgg gcgtgggccc gcaccaggcc gtggatccga taccgacggt
     1921 cacccaccac gtcgaggagg tgccgctctg ccagctcctc gaggcaccgc agcgcgtcgc
     1981 gctccgacga accccacagc gctccggcag attcgggcga cagcacgctg ctcggcagga
     2041 gacccagatc ccggtagcgg ccggccagat catccggcag ctccccgtac gccatgtcga
     2101 agatccccac gatgggttcg gcgtcacggt catccaaggt cgcgttgagg tccgcaatgg
     2161 tccgcgacgg ccgccgcacc agccatgcgg cacacatcct cagtgccagc gggaggcctc
     2221 cgcagtggtc gcggagcgcc tgcacgtccg gtgacccggc gacgacacga gcagggccga
     2281 tcatctcgat gagcagcagc tctgcatgat cgggcgacag ggggagcatc tccaccacgc
     2341 ccgcaccatc gacgatcagc tcctcgaggt agccctggct ggtgaccacc accaccgccc
     2401 cagaggagct cgggatgagg ggccggacct cagcgtcgtg cgcaacgtcg tcgaggaaca
     2461 cgagcatccg catctcggcg gtcgcgctgc ggtacaaggc ggtccgctca gcgagacccg
     2521 gcgggatggc agcgtccggc agccccaacg ctcgcagaaa gccgccgagc acgtcgctga
     2581 cccccacccc gccgccgacg cgaaggtctc cgaagttcgc gtacaagtgg gcctcgtcga
     2641 acgtcgcgcc gaccgtgtgg gcccagtacc ggccgattgc gctcttgccc actccgggca
     2701 tgccggtgat caccgccacg gccggcccaa ggcctctgtg ctccaccaga tcatcgaggg
     2761 ccgccatctc gaccgcccgg ttgacgaact gcttcggcga gggaggcagc tggcgcggcg
     2821 aaggcagccc gtctggatgg ggaacgatca gcgacaagac gccgatgtcg cgcgcctgga
     2881 cgctgacgtg cgcctcacct gagaaccagt tgcgtggccc agccgacggt gaactgccgt
     2941 cgctcaacgg cacctccatg aacgccgtcg cctccccatc gccgagtagc aggtcccagg
     3001 agcatacgcg tcgatgacga tgacgagcgc ccgcatcagc aactccgcgc cgatgccgaa
     3061 ccgacctgtc acccctttga cgctccccgg agcaggctcg ccacacgggc gacggaagcc
     3121 ctcaagacct gcagggtctg ctggacctgc gatgggctgg cgagttgctc agggatctcg
     3181 gacctgtccc agatcagatg ccctgcagtg agctcaccga gggccccgaa gccagaaaat
     3241 gaaccatgcc caggtctgtg acctgggcat gttccgtgtc ggagggggga cttgaacccc
     3301 cacgccccta agggcactag cccctcaagc tagcgcgtct gccaattccg ccactccgac
     3361 gtggctggcc gggcgaaccc ggccggaggg tgaccttagc cgaccgggtg ggccggccaa
     3421 gaacccggat cgctctccgt caggccggag tcgagaggaa cagccccagg gagagggtgg
     3481 tgaaggtgaa gcacaccgcc gcgatcaccg tggcccggtc gaggttcttc tccacgaccg
     3541 tggagccctg cgccgccgcg cccatgccgc cgccgaacat gtccgacaga ccgccgccct
     3601 tgccgctgtg caggagcacg aggaacagga gcaccagtga gtcgatcacg tggagtccga
     3661 ccaggaacca tttgaacggg gtcatgcaca agcctctcga gagcacgccc ctgtctccag
     3721 aggctggatc atggtagccg caccacccgg caaccccgaa tcagccggcc acaccgtcga
     3781 acgttcgatg cgaccactgc tcgaactcgc caaccacgag catcgaacgt cgggtggaca
     3841 ggcggccggg cggttcggat ggtcgggccg tccggtggtc gagtgagggc cggcggttgg
     3901 cggggtgacg acggggccga tggtcgagga acccaggagg acgggggtct ccaccgggtg
     3961 gtggccgtgt caggtgtggt tctgaaaatc cacgattccg gcgaaatctt cggcgacgag
     4021 ggacgcacct ccgaccagcg ccccatcgat atcgggtcga gccatcagct ctgccgcgtt
     4081 ggccggcttc accgaaccgc cgtactggat gcggacggat gcggccgcgt ccggcccggc
     4141 cacctccgcc accaccgagc ggacgagccg gcacgtctcc tgcgcgtcgt cggcactggc
     4201 ggtcttgccg gtgccgatcg cccagatcgg ctcgtaggcg atcaccatcg aaccgacctg
     4261 ctccgcggtc aggcccgcca gcccagcgcg cacctggccg gcgaccttct cctcggtgcc
     4321 ccccgcctca cgctcctcgt cggtctcgcc gcagcagagg atcggggtca tcccccactt
     4381 gaagatggcg tgcaccttct tgttgatcag ctcgtcggtc tccccgaaga tctcgcggcg
     4441 ctcggagtgg cccgcgatca cgtactcgac gttgagcttg gcgagcatcg ccggcgacac
     4501 ctcaccggtg aaggcgccct tctcctcgat gtagcagtgc tgggccccga tgctgatggg
     4561 gatgtcgtcg gcctcgagca ccgtctgcac cgaccggatg tcggtgaagg gcgggtgcac
     4621 ggacacgtcg acggtctcgt aggtgtcctt gtgcagcagg taggccagct tctggaccgt
     4681 ctggatggcc tcgaagtggt tgtggtgcat cttccagttg cccgagatga gcggcgtacg
     4741 ggtggacttg gccatggtgg actcctggtc cctgtcgatt tcgggtcacg cgtgcaccgt
     4801 cgtggtgctc aggtgacccg aaatgggctg gtggtgcgga cgatcgaggt ttcgggtcac
     4861 gcgtgcaccg tcgtggtgct caggtgaccc gaaatcgggt gctcaggatg cgttgggggc
     4921 gctgcggagg gcggcgaggc cggggaggtc gccctgctcg atgagctcga gcgaggcacc
     4981 gccgccggtg gagacgtggt cgatctggtc ggcgaggccg aactgggcgg cggcggcggc
     5041 gctgtcgccg ccgccgatga cggtgaagcc gcggcagtcg gccacggcct cggccaccgt
     5101 acgggtgccg gcttcgaagc ggggatcctc gaacacgccc atcgggccgt tccagaggac
     5161 ctggcgggct tcgccgatga cgtcggagaa ctccgccgcg gtgccggggc cgatgtcgag
     5221 gcccatccag ccgtccggca ggtccttgcc gaactgccgc acctctcccc cggccgacgg
//
LOCUS       DATMFS010000072         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_142336, whole genome shotgun sequence.
ACCESSION   DATMFS010000072 DATMFS010000000
VERSION     DATMFS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142336"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..801)
                     /locus_tag="VNQ33_01350"
     CDS             complement(154..801)
                     /locus_tag="VNQ33_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWJ96770.1"
                     /translation="MLDAVLFDLDGTLTDPAEGITGAFRHAVASVGHPADDDADLTWI
                     IGPSVRDNLARYGLPEEHHDLVVTTFRAHMTEIGLFQATVIAGITEVLASLHRADVPL
                     ALATAKPTDQAQTSLEHVALEAFFTVVSGNTAGAPISKSQIMANALDQLEHPDPARVA
                     MVGDRHHDIDGAHANGCIAVAVTWGYAEVGEMDVAKPHHIVDTPAQLGELLMRLP"
     gene            900..1127
                     /locus_tag="VNQ33_01355"
     CDS             900..1127
                     /locus_tag="VNQ33_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96771.1"
                     /translation="MATITVRLDADDERMLDELAEIHGDRSTVVREAIRQMAASETRR
                     EGLGAFLDDWERELGPIDHAQVDEIVRRLQL"
     gene            1145..1504
                     /locus_tag="VNQ33_01360"
     CDS             1145..1504
                     /locus_tag="VNQ33_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96772.1"
                     /translation="MISIDRGEEQAGATLRAFEQAGERMATTEPVLAQVWRDGARQAR
                     LARALQEVEIRPFTEGRRVGLLLGASGTRDVVDAHLVLVAVQTGEAILTGDPDDLGRL
                     AATFGERAPVIHPWPVI"
     gene            complement(1575..1651)
                     /locus_tag="VNQ33_01365"
     tRNA            complement(1575..1651)
                     /locus_tag="VNQ33_01365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1615..1617),aa:Pro,seq:cgg)
     gene            complement(1705..2124)
                     /locus_tag="VNQ33_01370"
     CDS             complement(1705..2124)
                     /locus_tag="VNQ33_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ96773.1"
                     /translation="MPRVLLATDADWIHEEVDAALAGDDIEVLRVREGLDVLPAVHEL
                     APDLVVLDLQIGNMGGMAACMNLRLDESVDRLPHIEVLMLLDRDVDAFLANRAEADGW
                     IVKPLDAFRLRRAATALLAGERYEEGMATSADVGTGS"
     gene            2270..>2458
                     /locus_tag="VNQ33_01375"
     CDS             2270..>2458
                     /locus_tag="VNQ33_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ96774.1"
                     /translation="MLEIEVDHTDSHTLCRPVGELDAYTVASFRDALTELAHQPRVVI
                     DLSDVPFMDSAGLGALIGG"
BASE COUNT          359 a          833 c          889 g          377 t
ORIGIN      
        1 cagggcgctt catgggttgc tccttggggg gaagggtcca gacctcaggg cggggccggc
       61 ccgtcaccgg tgcctccccg gggtggcggt ctcttcccac cagtcacgaa gatgaaacac
      121 agatcggtgg cgtcgccacc cgatgaggct gggtcagggc agccgcatga gcagttcgcc
      181 cagctgcgcg ggcgtgtcca cgatgtggtg cggcttggcg acgtccatct cgcccacctc
      241 ggcatagccc caggtgaccg cgacggcgat gcagccgttg gcgtgggcgc cgtcgatgtc
      301 gtggtgccgg tcgcccacca tcgccacccg ggccgggtcc gggtgctcga gctggtccag
      361 ggcgtttgcc atgatctgcg acttcgagat cggcgccccc gccgtgttgc cggatacgac
      421 ggtgaagaac gcctccaagg cgacgtgctc gaggctggtc tgggcctggt cggtgggttt
      481 ggcggtggcg agcgcgagcg ggacgtccgc gcgatggagc gaggccagca cctcggtgat
      541 cccggcgatg acggtggcct ggaacaggcc gatctcggtc atgtgggctc ggaacgtcgt
      601 caccacgagg tcgtggtgct cttccggcag cccgtagcgg gcgaggttgt cgcgcaccga
      661 cggcccgatg atccacgtga ggtcggcgtc gtcatcggcg gggtggccga ctgaggcgac
      721 cgcatgccgg aaggcgccgg tgatcccctc ggccggatcg gtcagcgtcc catcgaggtc
      781 gaacagcacg gcatcgagca tccgccgacg gtaccggccg gtcgttcgcc actcatcagg
      841 cgttgcgctc gcggaccgcc tgctcgacgc tgtcgtacat gcgtagtaca accatcgcca
      901 tggccacgat cacggtccgc ctcgacgctg atgacgaacg gatgctcgac gagctcgccg
      961 agatccacgg tgaccgctcg acggtcgtga gggaggcgat ccgccagatg gcggcgagcg
     1021 agacgcgccg ggagggcctc ggggccttcc tcgacgactg ggagcgggag ctgggcccga
     1081 tcgatcatgc ccaggtcgac gagatcgtcc gccgtctcca gctgtgatcc tcgacgctgg
     1141 tgtcttgatc agcatcgacc gaggcgagga gcaggccggc gcgacgctgc gggccttcga
     1201 gcaggcgggt gagcggatgg cgaccacgga accggtgctc gcccaggtgt ggcgcgacgg
     1261 ggcgcggcaa gctcgcctgg cccgcgcgct gcaggaggtg gagatccgtc cgttcaccga
     1321 gggcaggcgg gtgggcctgc tgctcggtgc gagcgggacg cgagacgtcg tggatgccca
     1381 cttggtcctg gtggcggtcc agaccggtga ggcgatcctg accggagacc ccgacgacct
     1441 cggccgcctg gccgcgacgt tcggcgagcg ggctcccgtc atccatccgt ggccggtcat
     1501 ctgaggctga ccgtcgcaac ctcgaagcga cggaaccccg ctcgacgagc ggggttccgt
     1561 gatgaccatg ggtctggtcg gggtggcggg atttgaaccc acgacctctg cgtcccgaac
     1621 gcagcgctct accaagctga gccacacccc ggagttgtgc ggagggtcac cgtagcccgt
     1681 cggccggtgg acctcctatc ccgttcagct cccggtgccg acgtcggccg acgtcgccat
     1741 gccttcctcg tagcgctcgc cggcgagcag cgccgtcgcc gccctgcgga ggcggaacgc
     1801 gtcgagcggc ttgacgatcc agccgtcggc ctcggcgcgg ttggcgagga acgcatccac
     1861 gtcgcggtcg aggagcatca ggacctcgat gtgcgggagg cggtcgacgg actcgtcgag
     1921 gcggaggttc atgcacgccg ccatgccgcc catgttgccg atctgcaggt ccaggaccac
     1981 caggtcggga gccagctcgt gcaccgcggg gagcacgtcg aggccttcgc ggacccggag
     2041 gacctcgatg tcgtcacccg cgagggcggc atcgacctcc tcgtggatcc agtcggcatc
     2101 ggtggcgagc agcacgcgtg gcacggcggc cacgctagcg cccggccctc cggcggctcc
     2161 atcggcccga accgccaggt cacgtggcgg gaacggaacg cggggttacg ccgtcagagg
     2221 ccatgggtac catcgggtct caccccgtct cacgaatgga cgctcctgcg tgctcgagat
     2281 cgaagtcgac cacaccgaca gccacacgct ctgccgcccc gtcggcgagc tcgacgccta
     2341 cacggtggcc agcttccgag acgccctcac cgagctggcc caccagcccc gggtggtcat
     2401 cgacctctcg gacgtgccgt tcatggattc ggccggcctg ggcgccctca tcggcggc
//
LOCUS       DATMFS010000073         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_72411, whole genome shotgun sequence.
ACCESSION   DATMFS010000073 DATMFS010000000
VERSION     DATMFS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72411"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..590)
                     /locus_tag="VNQ33_01380"
     CDS             complement(135..590)
                     /locus_tag="VNQ33_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HWJ96775.1"
                     /translation="MSSGPRRPAPTRDLDSPAEIAEMVRRFYQDVAQDDLLGPMFNDV
                     AQVDWVEHLPKLTAFWCRALLGIEGYAGNPFRAHAIVNDQAPFTHAHFQRWLDLFHDA
                     VELGWTGPNAQRALDLAHNVARVHAGQLVVEPGPSGATGATTVTIGDAP"
     gene            complement(587..1315)
                     /gene="ric"
                     /locus_tag="VNQ33_01385"
     CDS             complement(587..1315)
                     /gene="ric"
                     /locus_tag="VNQ33_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HWJ96776.1"
                     /translation="MDLAIDPSTTLADLVTSHPDLAGELERRSLDYCCGGQRTLAEAC
                     RAHGLDPEATALELAGASTATGPSPWASLDAAALVDHIEAEHHAYLHRELPRLRALAA
                     KVHGVHGAAHPELTAVVSTFGQLHAELEPHLAKEERVLFPLIRQLAADRPEEPRMGLT
                     GPISQMLREHDHAGELLERLRRSTDGYRVPPDGCASYGALYRGLEALEADTHLHVHIE
                     NNRLFPMVAGLESGGGAAASRSAR"
     gene            1448..1714
                     /locus_tag="VNQ33_01390"
     CDS             1448..1714
                     /locus_tag="VNQ33_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWJ96777.1"
                     /translation="MKVWIDQDLCTGDGLCEEIAPDIFFGQDDGLFYVKESAVHFGEE
                     KRFDGVTNPAGAEGMARIPEPLLESAIEAAEECPGECIFIEVDG"
     gene            1752..>2087
                     /locus_tag="VNQ33_01395"
     CDS             1752..>2087
                     /locus_tag="VNQ33_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWJ96778.1"
                     /translation="MNEAVKEIIDREYAYGFHTDLDTEVAPKGLDEDIVRFISARKDE
                     PRWLLEWRLSAYRHFITLDEPTWPRLDIAPIDYQDMHYWAAPKEKGPAPESLDDIDPE
                     VRAMFDKLGI"
BASE COUNT          316 a          695 c          768 g          308 t
ORIGIN      
        1 aggcgtggac aggtcgaggg gcggggcggg aggctgatct cggtggtcgg ggacctgtcc
       61 ctcccagcag ggcgggtcgc cgccgcacgc ctcgacgccg agcggaggcc ggcgcggacc
      121 gggggcctcg gccctcacgg cgcatcgccg atcgtgacgg tggtcgcgcc cgtcgcgccg
      181 gagggtcccg gctcgaccac gagctgaccg gcgtggaccc gcgccacgtt gtgggccaag
      241 tcgagcgccc gctgcgcgtt cggcccggtc catccgagct cgacggcatc gtggaagagg
      301 tcgagccacc gctggaagtg cgcatgggtg aacggggcct ggtcgttgac gatggcatgg
      361 gcccggaagg ggttgccggc gtacccctcg atgcccagca gcgcccggca ccagaacgcc
      421 gtcagcttcg ggaggtgctc gacccagtcc acctgggcga cgtcgttgaa catcggaccg
      481 aggaggtcgt cttgggcgac gtcctggtag aagcgccgca ccatctcggc gatctcggcc
      541 ggcgagtcca ggtcacgggt gggcgccggc cgccggggcc ccgagctcac cgtgcactcc
      601 tgctcgccgc cgcgccaccg cccgattcga ggccggcgac catcgggaac aggcggttgt
      661 tctcgatgtg gacgtgcagg tgggtgtcgg cctcgagggc ctcgagaccc cggtagagcg
      721 cgccgtagct ggcacagccg tcgggcggca cgcggtagcc gtcggtcgag cggcgcaggc
      781 gctcgagcag ctcccccgcg tggtcgtgct cgcgcagcat ctgcgagatg ggtcccgtca
      841 gacccatccg gggttcctcg ggacggtcgg cggcgagctg gcggatcagc gggaacagca
      901 cccgctcctc cttggcgagg tgaggctcca gctccgcgtg cagctgcccg aacgtggaca
      961 cgacggcggt gagctcgggg tgcgcggcgc cgtgcacgcc gtgcaccttt gccgccagcg
     1021 cgcgcagccg agggagctcc cggtgcaggt aggcgtggtg ctcggcctcg atgtggtcga
     1081 cgagggcggc ggcgtcgagc gatgcccacg gcgagggccc ggtggccgtc gacgcgccgg
     1141 cgagctcgag cgcggtggcc tcgggatcga gcccgtgggc ccgacaggcc tccgcaagcg
     1201 tgcgctggcc gccgcagcag tagtcgagcg agcggcgttc gagctcgccg gccaggtcgg
     1261 ggtggctcgt cacgaggtca gcaagggtgg tcgacgggtc gatggccagg tccatgggag
     1321 cagcgtacct atttttctag tgattactag agtcgttggt caccacgcgc gacccgcatc
     1381 ggcctggcgt ttctttttct agtctgtact agtattattt ggagtaccag cgaacccggg
     1441 agcgcccatg aaggtttgga tcgaccagga cctctgcacc ggtgacggtc tctgcgagga
     1501 gatcgctccc gacatcttct tcggacaaga cgacgggctc ttctacgtga aggagtccgc
     1561 cgtgcacttc ggcgaggaga agcgcttcga cggggtcacg aacccggcgg gcgcggaggg
     1621 gatggcccgc atccccgaac cgctcctgga gtcggcgatc gaggcggccg aggagtgccc
     1681 gggcgagtgc atcttcatcg aggtggacgg atgacccggg acgttcgagc gcacgggccg
     1741 ggaccacgct cgtgaacgaa gccgtcaagg agatcatcga ccgggagtac gcctacgggt
     1801 tccacaccga cctcgacacc gaggtcgcac cgaagggcct cgacgaggac atcgtccggt
     1861 tcatctcggc ccgcaaggac gaaccgcgct ggctgctcga gtggcgcctg tccgcctacc
     1921 ggcacttcat cacgctcgac gagccgacct ggccccggct cgacatcgca ccgatcgact
     1981 accaggacat gcactactgg gcggccccca aggagaaggg ccccgcgccc gagagcctcg
     2041 acgacatcga ccccgaggtc cgggccatgt tcgacaagct cggcatc
//
LOCUS       DATMFS010000074         3136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_72422, whole genome shotgun sequence.
ACCESSION   DATMFS010000074 DATMFS010000000
VERSION     DATMFS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72422"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..1960
                     /locus_tag="VNQ33_01400"
     CDS             401..1960
                     /locus_tag="VNQ33_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWJ96779.1"
                     /translation="MNSDPAGRHDDVMRTEMSRRRVLRGMLATAGVVAVGAGIDGWAA
                     QGPARAAGAILPPGTRPNPSLPEGVDTLPEIEHIIIYMQENHSYDQYFGMLGRGDGFT
                     LGPDGLPTNANPDANGNPVRAFYAGPTCDTNIGGDHSWNAEHLAVNGGTMDGFIRGNG
                     GDPRIMGYYDDRNLPFYWGLAKTFPICDRWFCSVRGPTHPNRRFLQAGTSAGIVQTSV
                     PEVLATPNAPNGTIWDRLDDHRISWKNYMFDLGDIYLFPGSDIAKFNARTADNRRTFS
                     DFLLDCQKGTLPAVSILAPALSGWYDEGARDVQNGEAYSAAIINAVMSSPSWSKSIVL
                     FTYDENGGGYDHVAPPPAVAPDNIAPRIDAGDQPGDFTNLGPRVPGFVISPFSKANYV
                     SHVVHDHTSILRFIETKYNLGAITHRDANASDLLDSLDFANPGFLDPPDLPAPGLPAT
                     GSVCEPQPYPAFNPKDVTTTSTTTPTVTTTTAPTGSEGTRGSTTLPASLNSLMDAPPA
                     QPVRGDANFTG"
     gene            complement(2147..>3136)
                     /locus_tag="VNQ33_01405"
     CDS             complement(2147..>3136)
                     /locus_tag="VNQ33_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWJ96780.1"
                     /translation="YTTTRDEGEPAIASGLVVVPAASSSTPLPVVAWAHGTTGIDRNC
                     APSVVERTFESGAFFSLDKVLHEGWALVATDYVGLGTKGPHPYLIGQGEARSVLDAVR
                     AARQMPDARLAGETVVWGHSQGGNAALWTGQIAPVYAPDVPLSGVAALAPASDLPALV
                     TTLPKVSIGSLFATYVIQAYADTYPEIQVSEYVRPAARTTFDEAAKRCLDATALASVA
                     SSLAVGFEGFDDVSSGPLHERLVQNTPTGPISAPLLLAQGEADGLIVPSAQAAYVQRL
                     CSAGQPVDFRTFPGLDHVPLVEADSPLIPQLLAWTEARFGGEPATPTCGPGAG"
BASE COUNT          520 a         1175 c         1011 g          430 t
ORIGIN      
        1 cgccgtcttc cggtccacac ttgccgcggc cacgtggtac gggatcggcc tcgccgtcat
       61 gacctccaac gtcgcaaccc gcatccagac ctcgccgccc gagccggcca ccgactccgg
      121 cccaagtccg ctggacgcac ggggatacct ctagccgtcg atgggtcggt ccgatgtctt
      181 gtggccacga agttccggac aggcacccca tccgggaggg cgacccaccg atccaccacc
      241 cgaaccgtcc gccggcgtct cgtcacctac ggcaaacacc gagtgttcag gtcgaattca
      301 ccggaacgcc gagtcaccga gccctcacgc cagccgcaga ccagtagcgt cggcgccggt
      361 ttgtggccct tccaccatgg ccaggggagg aatgatgatg atgaacagcg atccggctgg
      421 acgccacgac gacgtcatgc ggaccgagat gtctcgtcgg cgcgtgctgc ggggcatgct
      481 cgccaccgcg ggggtcgtcg ccgtcggtgc cggcatcgac ggctgggccg cgcaggggcc
      541 tgctcgtgcc gcgggcgcga tcttgccgcc gggcacccgg ccgaatccgt cgctgcccga
      601 aggcgtcgac accctcccgg agatcgaaca catcatcatc tacatgcagg agaaccactc
      661 ctacgaccag tacttcggca tgctcggccg cggcgacggg ttcaccttgg ggcctgacgg
      721 cctgcccacc aacgccaacc ccgatgccaa cgggaacccg gtgagggcct tctacgccgg
      781 ccccacctgc gacaccaaca tcggcggcga ccactcgtgg aacgccgagc acctggccgt
      841 gaacggcggg accatggacg gcttcatccg cggcaacggc ggtgaccccc gcatcatggg
      901 gtactacgac gaccgcaacc tgcccttcta ctggggcctg gccaagacct tcccgatctg
      961 cgaccgctgg ttctgctcgg tccgcgggcc gacccatccc aaccgccgtt tcctgcaggc
     1021 cggcacgtct gcggggatcg tgcagacgag cgtccccgag gtgctcgcga cgccgaacgc
     1081 gccgaacgga accatctggg accgcctcga cgaccaccgc atcagctgga agaactacat
     1141 gttcgacctg ggcgacatct acctcttccc cgggtccgac atcgcgaagt tcaacgcccg
     1201 tacggcggac aaccgccgca cgttctcgga cttcctcctc gactgccaga agggcacgct
     1261 gccggccgtc agcatcctcg ctcccgcctt gagcggttgg tacgacgaag gagcccgcga
     1321 cgtccagaac ggcgaggcct acagtgcggc catcatcaac gccgtgatgt cgagcccctc
     1381 gtggtcgaag tccatcgtgt tgttcacgta cgacgagaac ggcgggggct acgaccacgt
     1441 ggccccgccg ccagcggtcg cgcccgacaa catcgccccg cgcatcgacg ccggcgacca
     1501 gccgggcgac ttcacgaacc tcggcccccg cgtgcccggg ttcgtgatct cgcccttctc
     1561 gaaggcgaac tacgtgtcac acgtggtgca cgaccacacc tcgatcctcc ggttcatcga
     1621 gaccaagtac aacctgggcg ccatcaccca ccgggatgcg aacgcgagcg acctgctcga
     1681 ctccctcgac ttcgcgaacc cgggcttcct cgacccgcca gacctgcccg caccgggcct
     1741 gcccgccacc ggcagcgtct gcgagccgca gccgtacccc gcgttcaacc ccaaggacgt
     1801 gaccacgacc tcgacgacca cgccgaccgt gaccaccacg acggcgccca ccgggtccga
     1861 agggacccgc gggagcacca cgctgccggc atcgttgaac tcgctgatgg acgctccacc
     1921 tgcccagccg gtgcgcggcg acgccaactt caccggttga ccaggcaccg gtcgtggacc
     1981 tcgcgctccg ggtcttgcgt ccctgctgac cttccccggc gagacccggc atcggaacgc
     2041 acgacgaagg aacccgggga ccgctcctgt gatcgcggca cgggtgagac cttgccgctg
     2101 gtcgggcgga agaccacact cagcctcgga ggcggcggca gcgcgatcat ccggcgcccg
     2161 gcccgcaggt cggcgtcgcc ggctcgccgc cgaagcgggc ctcggtccac gccagcaact
     2221 gggggatgag cggcgagtcg gcctcgacca acggcacgtg gtccaggccc gggaaggtcc
     2281 ggaagtcgac cggctgacca gccgaacaca gccgctggac gtacgcagcc tgggcggacg
     2341 ggacgatgag accgtcggcc tcgccctgcg ccagcagcag cggagcgctg atcgggcccg
     2401 tcggggtgtt ctgcaccagg cgctcgtgca ggggaccgct cgacacgtcg tcgaaccctt
     2461 cgaagccgac ggcgagcgac gaggcgaccg aagcgagcgc ggtcgcgtcg aggcatcgct
     2521 tcgcggcctc gtcgaacgtg gtgcgcgccg cgggcctcac gtactcgctg acctggatct
     2581 cgggataggt gtcggcgtac gcctggatca cataggtggc gaacaggctg ccgatcgaca
     2641 ccttcggcag cgtcgtgacc agcgccggca ggtcgctcgc cggggccagc gccgccacgc
     2701 cgctgagcgg cacgtcgggg gcgtagacgg gggcgatctg tccggtccac aaggccgcgt
     2761 tgccaccctg cgaatgtccc cagaccacgg tctcaccggc caggcgcgcg tcgggcatct
     2821 gccgcgccgc gcgcacggcg tcgagcaccg agcgcgcttc gccctgaccg atgaggtagg
     2881 ggtgcggccc cttcgtgccc aacccgacgt agtcggtggc gacgagcgcc cagccctcgt
     2941 ggagcacctt gtcgagcgag aagaacgcac cggattcgaa cgtccgctcc accaccgatg
     3001 gggcgcagtt ccggtcgatg ccggtggtgc cgtgcgccca cgccaccaca gggagcggcg
     3061 tcgacgagga ggccgccggc accaccacca gaccgctcgc gatggcgggc tccccctcgt
     3121 cgcgggtggt ggtgta
//
LOCUS       DATMFS010000075         3106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_3621, whole genome shotgun sequence.
ACCESSION   DATMFS010000075 DATMFS010000000
VERSION     DATMFS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VNQ33_01410"
     CDS             <1..664
                     /locus_tag="VNQ33_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HWJ96781.1"
                     /translation="PFGHGLSYTTFADEDLVAQPLDHTGADAEPGDWRGAPVLEVSAT
                     VANTGPVAGKEVVQLYVGAPDASVSRAVRELKGFAKITLDPGQAETVVFTLHERDLSY
                     WSTRTHGWVLEPGTFEIAVGASSRDLRLTRTVEVDGPPIAYPLDRSSTLAEWLDHPVG
                     HEILIDVLRHSRAGDLSGIAKDPERVRMLGSFPVTRLTTLFGPTLDGDVVDQLLARLG
                     EA"
     gene            703..2115
                     /locus_tag="VNQ33_01415"
     CDS             703..2115
                     /locus_tag="VNQ33_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWJ96782.1"
                     /translation="MTEAPSDRRPNVILVNCDDLGYGDLGCYGSTRNATPAIDQLAAE
                     GLRFDDFYMASPVCSPSRGAMLTGCYPPRIGFDSFDGLPVLFPGQGLGLPTSEISLAT
                     ILRGAGYRTQMVGKWHCGDQPGFLPTDHGFDHWFGLPYSNDMGRQTHPAEGPLAPPPG
                     TEPFFLNPLPLMLDDAVIEQQPDQAALTERYVTESVRFIRDARDEPFFLYFAHLYVHI
                     PLYVQPRFATGSQNGDYGAAVACIDWALAALLHELRELGLDEDTIVIFTSDNGSRGVD
                     GGSNAPLRGAKTTTWEGGMRVPCIVRWPGTVAPGRTTAEVATAMDLYPTLAAVCGAEV
                     PTDRTIDGRDLAPLLRDDAATSPHDALLYYWMDQLEAVRVGRWKVHYAKHGVEQIELY
                     DLEADPAETTDVAAGHPEVLAELAVHAEAARASLGDRLQGRVGADVRPIGRVADPVTL
                     TSYDPDHPYYLAEYDLTDRG"
     gene            2286..>3106
                     /locus_tag="VNQ33_01420"
     CDS             2286..>3106
                     /locus_tag="VNQ33_01420"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWJ96783.1"
                     /translation="MERAHRDASRPAASGGRNQTSTALAAVCFAALAVLSATVAVAVV
                     GSPAASAAAPTVVHLSASTMPAFQSIPVTVTATVSSPSAPGDASMTGTVRFNGHTHCL
                     DQPIVNGVATCQTASSNQTYFPFTATYSGDENWASSTSEVFGWRSSRNGIDWRIEPDM
                     DQTCGAFRSLELPDVAGAIPGATTATITLEGGNGGTSSISLGGASSTRGRGGEASLQG
                     VPYPIAGSTHLSYIIGCAGQSVFPATTAARGGAGWGDGGRGGAPTGSAPRGGGGGG"
BASE COUNT          459 a         1205 c         1017 g          425 t
ORIGIN      
        1 cccgttcggc cacggcctgt cgtacacgac gttcgccgac gaggacctcg tcgcccagcc
       61 gctggaccac accggtgccg acgccgaacc gggcgactgg cggggcgctc ccgtcctgga
      121 ggtgtcggcc acggtcgcca acaccggacc ggtcgccggc aaggaggtcg tgcagctcta
      181 cgtcggcgcc cccgacgcat cggtctcccg agcggttcgg gagctgaagg gcttcgccaa
      241 gatcaccctc gatcccggcc aggccgagac ggtggtcttc acgctgcacg agcgggacct
      301 gtcgtactgg tcgacgcgca cccacggctg ggtgctcgaa ccgggcacgt tcgagatcgc
      361 cgtcggcgcc tcgtcccgcg acctgcggct cacccgcacc gtcgaggtcg acgggccgcc
      421 gatcgcctac ccgctcgacc gcagcagcac cctggccgag tggctcgacc acccggtggg
      481 ccacgagatc ctgatcgacg tgctccgcca cagccgcgcc ggcgacctga gcggcatcgc
      541 caaggatccc gagcgggtcc gcatgctcgg ctcgttcccg gtgacccgcc tcacgacgct
      601 gttcggtccg acgctggacg gcgacgtggt cgaccagctc ctcgcccgcc tcggcgaggc
      661 ctgaccggcc gcgcccggcg gtgcgggcgc tagaactccc tcgtgaccga agctccatct
      721 gatcgccggc cgaacgtcat cctggtcaac tgcgacgacc tcgggtacgg cgacctcggc
      781 tgctacggct ccacccgcaa cgccacaccg gccatcgacc agctcgcggc cgaaggactc
      841 cggttcgacg acttctacat ggcgtcgccg gtctgctcgc cgtcccgcgg cgccatgctc
      901 accggctgct acccgccgcg gatcggcttc gactccttcg acgggctgcc ggtgctgttc
      961 ccgggccagg gcctcgggct gcccaccagc gagatcagcc tggcgacgat cctgcgcggc
     1021 gccggctacc ggacgcagat ggtgggcaag tggcactgcg gagaccagcc tggcttcctc
     1081 cccaccgacc acggcttcga ccactggttc ggcctgccct acagcaacga catgggccgc
     1141 cagacgcacc cggccgaagg gccgctcgct ccgccgccgg gaaccgagcc cttcttcttg
     1201 aacccgctcc cgctgatgct cgacgacgcc gtgatcgagc agcagcccga ccaggcggcg
     1261 ctcaccgagc gctacgtcac cgagtccgtc cgcttcatcc gggatgcccg tgacgaaccg
     1321 ttcttcctct acttcgccca cctctacgtg cacatcccgc tgtacgtgca gccccggttc
     1381 gccaccgggt cccagaacgg cgactacggc gccgcggtcg cctgcatcga ctgggcgctc
     1441 gccgcgctcc tccacgagct gcgggagctc ggcctcgacg aggacacgat cgtgatcttc
     1501 acgagcgaca acggctcccg tggcgtcgac ggcgggagca acgcgccgct gcgcggcgcc
     1561 aagaccacca cgtgggaggg cggcatgcgc gtcccgtgca tcgtccgctg gccgggcacc
     1621 gtcgcacccg ggcgcaccac cgccgaggtg gcgacggcga tggacctcta cccgaccctc
     1681 gcggcggtgt gcggcgccga ggtccccacc gaccggacga tcgacggccg cgacctggcg
     1741 cccctgctcc gagacgacgc cgccacctcg ccgcacgacg cgctcctcta ctactggatg
     1801 gaccagctcg aggcggtgcg ggtcggtcgg tggaaggtgc actacgccaa gcacggcgtc
     1861 gagcagatcg agctgtacga cctggaggcc gatccggccg agacgaccga cgtcgccgcc
     1921 ggccaccccg aggtgctggc cgagctggcc gtccacgccg aagcggcccg agcatcgctg
     1981 ggcgaccgcc tgcagggccg cgtcggtgcc gacgtccggc ccatcggccg cgtcgccgac
     2041 cccgtcaccc tcacctcgta cgacccggac cacccctact acctggccga gtacgacctc
     2101 accgaccgcg gctgacgtcc cctggccgtc cggtggtcgc gcacgcggcc ggccgagaac
     2161 gaatcgccac gccgctcgat ccccctcccg gaggtgcgtc acgttgcgcg ccttgcacct
     2221 cgcccgacct gcggttctga cctagcgtac gaacccagat cggttgggta ccagggggac
     2281 gatcaatgga acgggcacac cgggatgcgt ctcggcctgc tgcgagcggc ggccgcaacc
     2341 agacctcgac cgcgctcgcg gccgtctgct tcgcggcact cgccgtgctc tcggccaccg
     2401 tggccgtggc ggtcgtcggc tcgcccgctg cgtcagcggc ggcccccacc gtcgtccacc
     2461 tcagtgcgtc gacgatgccc gccttccaga gcatccccgt gaccgtgacc gccaccgtgt
     2521 cgagcccgtc ggcaccgggc gacgcgtcga tgacgggaac cgttcgcttc aacggacaca
     2581 cgcactgcct cgaccagccc atcgtcaacg gggtcgccac ctgtcagacc gcgtcgagca
     2641 accagaccta ctttccgttc acggcgacct attcgggcga cgagaactgg gccagctcca
     2701 catcggaggt cttcgggtgg cggtcgagcc ggaacgggat cgactggagg atcgaaccgg
     2761 acatggacca gacctgcggc gcgttccgct cgctggagct ccccgacgtc gccggagcga
     2821 tcccaggcgc caccacggcg acgatcaccc tcgaaggggg caacggagga acgtcttcca
     2881 tcagcctcgg cggggccagc tccaccaggg gtcgaggagg ggaggcatcg ctccaaggtg
     2941 tcccgtatcc gatcgcaggg tccacccacc tctcctacat catcggatgt gcaggccaga
     3001 gcgtcttccc ggcaaccacc gctgccaggg gcggcgcggg ctggggcgac ggcggacgcg
     3061 gcggcgcccc gaccgggagc gcgccccgag gcggaggagg aggggg
//
LOCUS       DATMFS010000076         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_49392, whole genome shotgun sequence.
ACCESSION   DATMFS010000076 DATMFS010000000
VERSION     DATMFS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49392"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1054
                     /locus_tag="VNQ33_01425"
     CDS             122..1054
                     /locus_tag="VNQ33_01425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWJ96784.1"
                     /translation="MDLRIFTEPQQGAAYDTLLAVAREAERLGFDAFFRSDHYLKMGD
                     VAGDPGPSDAWITLAGLARDTTTIRLGTLVTAATFRLPGPLAISVANVDAMSGGRVEL
                     GLGSGWFDAEHEAYGIAFPSLKERFDRYEEQLAIITGLWTTPEGETFSFDGEHYQLTE
                     SPALPKPVQSPRPPILIGGGGAKRTPRLAATYADEFNVPFSSLDDTSAAFDRVRAACE
                     ARDRDPGSLVYSAAQVVCVGADEAEVQRRAAAIGRQPDELRANGAAGTPDEVVARLRS
                     FAGLGATRAYLQVLDLSDLDHLALIAEEVAPQLR"
     gene            complement(1086..2585)
                     /locus_tag="VNQ33_01430"
     CDS             complement(1086..2585)
                     /locus_tag="VNQ33_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ96785.1"
                     /translation="MDAPEAPTRDGVDDAPGLRYGTPQAWWTIGAVVLGSGIVFLDGT
                     VVNVALPSIGQDLGTSLGGMQWTVNAYLVTLSSLLLLGGSMGDQFGRRRVFVAGLVVF
                     TAASMLCGVAPTTGFLVAARALQGIGGALLVPGSLSILAATFHPDDRARAIGAWSGMA
                     GVTSSLGPFLGGWLIDAFTWRLIFFINVPLAAVAVAVAVRHVPETRAPVRLPLDLAGA
                     ALVTVALAAISYAAIEQGASAQGAVAVVGVVALIGFLAWEHRSAHPMLPLGVFRSRQF
                     SGANLTTFAVYAALGAAFFLVTLQLQISLGYSALEAGVSFFPFTLLMLWLSPKAGQVS
                     QRIGPRLPMTIGPLTAGAAMVVFGQIEPGDRYVTSVLPAVVLFGLGMATTVAPLTATV
                     LASVDDAMAGVASGVNNAVSRLAGLLAVAVLPALAGIAIGDSLADALAEGYTTALRMS
                     AALCAAGGAIALVMVERARPTRPVVHPAPQVACGDPCLAESETARSDRN"
BASE COUNT          427 a          988 c         1044 g          317 t
ORIGIN      
        1 gcctcgtctc tcgtgcgtgg cggagacggt acggcgcgca tcgccgaccc cacaacgcca
       61 gcgtgcgaga tgtcgtgtag gaggcgcgcg ggagcgggca cgaaggcggt aggaagtcgg
      121 gatggatctg cgcatcttca ccgagcccca gcagggcgcc gcctacgaca ccctcctcgc
      181 cgtggcccgg gaggcggagc gcctcgggtt cgacgccttc ttccgatccg accactacct
      241 gaagatgggc gacgtggccg gtgaccccgg cccgagcgac gcgtggatca cgctcgccgg
      301 cctggcacgc gacaccacca cgatccgcct cggcacgctg gtgacggcgg cgacgttccg
      361 gctgccgggc ccgctcgcca tctcggtggc caacgtcgat gccatgagcg gggggcgcgt
      421 cgagctcggc ctcgggtcgg gctggttcga tgccgagcac gaggcctacg gcatcgcctt
      481 cccgtcgctc aaggagcgct tcgaccgcta cgaggagcag ctggcgatca tcaccggcct
      541 ctggaccacg cccgagggcg agacgttctc cttcgacggc gagcactacc agctcaccga
      601 gtcacccgcg ctgcccaagc cggtccaatc gccccgaccg cccatcctca tcggcggggg
      661 cggggccaag cgcacgccgc gcctggcggc cacctacgcc gacgagttca acgtgccgtt
      721 ctcgtcgctc gacgacacca gcgcggcgtt cgaccgggtc cgcgccgcgt gcgaggcgcg
      781 cgaccgcgat ccgggctcgc tcgtctactc ggccgcccag gtggtgtgcg tcggtgccga
      841 cgaggccgag gtgcagcgcc gcgccgcggc catcggacgc caacccgacg agctgcgggc
      901 gaacggcgcc gccggcacac ccgacgaggt cgtcgcccgg ctgcggtcct tcgccggcct
      961 cggcgccacc cgcgcctacc tccaggtcct cgacctgagc gacctggacc acctcgcgct
     1021 gatcgccgaa gaggtggcgc cccagctgcg ctgaacccgc tgcgttgaac ccactgcgct
     1081 cgtcgtcagt tgcggtcgga acgggccgtc tcgctctccg ccaggcacgg atcgccgcac
     1141 gcgacctggg gcgccgggtg cacgaccggc cgggtcggcc ttgcccgctc caccatcacg
     1201 agggcgatcg ccccgccggc ggcgcagagg gccgccgaca tccgcagggc ggtggtgtag
     1261 ccctccgcga gggcgtcggc cagcgaatcg ccgatggcga tgccggcgag ggccgggagc
     1321 acggccacgg ccaagagccc ggccaggcgg gagacggcgt tgttcacgcc ggaggcgacg
     1381 ccggccatgg cgtcgtcgac gctggcgagg acggtggccg tgaggggcgc gacggtggtg
     1441 gccatgccca agccgaacag cacgaccgcc ggcaggacgc tcgtcacgta gcggtcgccc
     1501 ggttcgatct gtccgaacac gaccatggcg gccccggcgg tgagggggcc gatggtcatg
     1561 ggcaggcggg ggccgatgcg ctggctcacc tggccggcct tcggtgagag ccacagcatc
     1621 aggagcgtga acgggaagaa cgacacaccc gcttcgaggg ccgagtagcc gagcgagatc
     1681 tggagctgca gggtcacgag gaagaaggcg gccccgagcg cggcgtagac ggcgaacgtg
     1741 gtgaggttgg cgccgctgaa ctggcgggag cggaacaccc cgagcggcag catcgggtgg
     1801 gcactgcggt gctcccaggc caagaagccg atcagcgcca ccacgcccac caccgcgacg
     1861 gcaccctggg cggatgcccc ttgctcgatg gcggcgtacg agatggcggc cagcgccacg
     1921 gtgacgagcg cggcaccggc gaggtcgagg ggtaggcgga ccggagcccg cgtctcgggg
     1981 acgtggcgca cggccacggc gacggcgacg gcagccagcg gcacgttgat gaagaagatc
     2041 agccgccagg tgaaggcatc gatgagccaa ccgccgagga acggcccgag cgagctggtc
     2101 acaccggcca tgccggacca ggcgccgatg gcccgagccc ggtcatcggg gtggaacgtg
     2161 gcggcgagga tcgagaggct gccgggcacg agcagggcgc cgccgatgcc ctggagcgcc
     2221 cgagccgcga ccaggaaccc ggtggtcggc gccaccccgc acagcatcga cgccgcggtg
     2281 aagaccacca ggccggcgac gaagacgcgg cgccggccga actggtcgcc catcgacccg
     2341 cccagcagca gcagcgacga cagggtcacg aggtaggcgt tgaccgtcca ctgcatgccg
     2401 cccagcgagg tgccgaggtc ctggccgatg gaggggagcg cgacgttgac gacggtgccg
     2461 tcgaggaaga cgatgcccga gccgaggacc accgccccga tcgtccacca ggcctgcggg
     2521 gtgccgtagc ggagccccgg ggcgtcgtcg acgccgtctc gggtcggggc ctccggtgcg
     2581 tccacccgcg ggagggtacc gaggcgccga ggcgtccggc gtcgtggagg ccctccggtc
     2641 ggccatcgtc ggtggagccg gtggcgccgg tggacgaggt caggccttgg cgagcagccg
     2701 gacgaccttg gcgccgccgt cgtgggcgag ggtcccgacc tccacgaacc cgcgcgcggc
     2761 gtggaacgcg agcgag
//
LOCUS       DATMFS010000077         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_235260, whole genome shotgun sequence.
ACCESSION   DATMFS010000077 DATMFS010000000
VERSION     DATMFS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235260"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VNQ33_01435"
     CDS             <1..604
                     /locus_tag="VNQ33_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96786.1"
                     /translation="ITIGIAAVAIVLSMAILATVNRPAPSGDDGLAATPGVKDVERPQ
                     NTDPFVLAESDTRGSTTLPFRNGTTAPPPPITTPIPTTTQVPLDAPAGDPVCQGALTL
                     GEMARRSAEFNNDLPQLREFVAQQMRAASAQFATAGGNYTTMSNLLDQRVPLVESAEA
                     VQDLIDLLLPLLRWTDPAVQPVAREMTEHLSNACPILSPG"
     gene            741..2279
                     /locus_tag="VNQ33_01440"
     CDS             741..2279
                     /locus_tag="VNQ33_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96787.1"
                     /translation="MISIDQTQTSADRHHDQLGRQGDRPAANLLRCVLWAAAAAAVAV
                     LLWQAGLAAGLAAAAAITGAAQLTTLVAPLSRQVGRIEASTLRWAETTTAQARAAPRR
                     WRGWRPRSRWWRARRAELAATFAVCSGLLAPIYLRLAHDPLAQVVTNNDVYLHIQVAE
                     KMQRWPPEIPHPVFHYLYLAGKPLLGVRWAPLVPVLVATAMMAAGLVRLGATSAHGRP
                     ALRGGAVVAFAAVVILTDSPTLLLSASGIIEPTPVMIPHVWWSPTMLLLFGSSLLLVP
                     FVAELVAAAAPTSPTHDRLRRLGPIVLALTVFATLSRPNLTFALVPGLLIYLVRDRRW
                     DRATLTAAMTWLIVPAGLVYLWQTSVVLDNYSSMRGLRINPFYEMAQFQMFVRPWFFY
                     LTAVPTVIAALYARKRFTRDPVVFLTLQAFWFALALMALLDDSQVEQNAHGVYVLPFT
                     SVWLVLWVLTVRFWVGELVAARRAGPAEARKFVIILAGTVVWVAAGVVAHLTAMGVHR
                     FAMI"
BASE COUNT          311 a          873 c          748 g          375 t
ORIGIN      
        1 catcaccatc ggcatcgctg cggtggccat cgtgttgagc atggccattc tcgccacagt
       61 gaaccgcccg gcgccgagcg gtgacgacgg cctcgcggcg acgccgggcg tgaaggacgt
      121 cgagcgcccg cagaacaccg atcccttcgt gctcgccgag tccgacaccc gcggttccac
      181 caccctgccc ttccgcaacg ggacgacggc gccgcctccc ccgatcacga cgccgatccc
      241 caccaccacg caggtccccc tcgatgcccc cgccggggat cccgtctgcc agggcgccct
      301 gaccctgggg gagatggccc gcaggagcgc ggagttcaac aacgacctgc ctcagctccg
      361 ggagttcgtc gcgcagcaga tgcgggcagc atcagcgcag ttcgccacgg ccgggggcaa
      421 ctacacgacg atgtccaacc tcctcgacca gcgcgtccca ctcgtcgaat ctgcggaagc
      481 ggtgcaggac ctcatcgatc tgctcctgcc gcttctgcgg tggaccgacc ccgcggtcca
      541 gccggtggcc agggagatga ccgagcatct cagcaatgcc tgccccatcc tgagccctgg
      601 gtgacagcag gggcatcgga tgcttcggtc ttggcgcggg cctgcgcccg cggagggagc
      661 accgtaggat ccccggcgct ccgctgcact tcgatgaagc ccgagtggaa gtcacgcctc
      721 ctgaacccca ggctcctccg ttgatcagca tcgaccagac ccagacgtcc gccgatcgtc
      781 accacgacca gctcgggcgc cagggcgacc ggccagcagc gaacctcttg cgatgtgtcc
      841 tctgggcggc cgcggccgcc gccgtcgccg tgctgctgtg gcaggcggga ctggcggccg
      901 ggctggccgc ggcggcggcc atcaccggcg cggcgcagct gacaacgctg gtggcgcccc
      961 tctcacgcca ggtcggccgc atcgaagcca gcacgctgcg ctgggcggag acgacgacgg
     1021 cacaggcccg cgccgcgccg cgccgctggc gcgggtggcg cccgcgcagc cgctggtggc
     1081 gagcacggcg ggctgagctc gccgcgacct tcgccgtgtg cagcgggctc ctcgcgccga
     1141 tctacctccg cctcgcgcac gacccactgg cccaggtcgt gaccaacaac gacgtctacc
     1201 tccacatcca ggtcgcagag aagatgcagc ggtggccacc ggagatcccc cacccggtgt
     1261 tccactacct gtacctggcg ggcaagccgc tcctcggggt gcggtgggcc ccgctggtcc
     1321 ccgtgttggt cgccaccgcc atgatggcgg cggggctggt gcgcttgggg gcgacatcgg
     1381 ctcacgggcg cccggccctg cgcggcggcg cggtggtcgc gttcgcggcc gtcgtgatcc
     1441 tcaccgacag ccccaccctc ttgctgtcgg cgtcgggcat catcgagccg acaccggtga
     1501 tgatccccca cgtgtggtgg agcccgacga tgctcctcct gttcggatcc tcgctcctgc
     1561 tcgtcccctt cgtcgccgag ctggtcgccg ccgcggcgcc gacctcgccg acccacgacc
     1621 gcctgcgccg gctcggcccg atcgtcctcg cgctcacggt gttcgccacc ctctcgcgtc
     1681 cgaacctcac ctttgcgctc gtgccgggcc tactgatcta cctcgtgcgc gatcggcgat
     1741 gggaccgtgc gacgctgacc gcggcgatga cgtggctgat cgtccctgcc gggttggtgt
     1801 acctctggca gacgtcggtc gtgctcgaca actactcgag catgcgcggg ctgaggatca
     1861 acccatttta cgagatggcc cagttccaga tgttcgtccg gccctggttc ttctacctca
     1921 cggcagtgcc gaccgtcatc gccgccctgt acgcgcgaaa gcggttcacc cgagaccccg
     1981 ttgtcttcct cacgctccag gcgttctggt tcgcccttgc gctgatggcg ctgttggatg
     2041 actcccaggt agagcagaac gcccacggcg tctacgtcct cccgttcacc tccgtctggc
     2101 tggtgctgtg ggtgctgacc gtccgcttct gggtcggcga gctcgtcgcg gcccgccgcg
     2161 ccggcccagc ggaagcccgg aagttcgtca tcatcctcgc cggaacggtg gtgtgggtcg
     2221 ccgccggggt cgtcgcccac ctcaccgcga tgggcgtgca ccggttcgcg atgatctgaa
     2281 gtatcgacgg tctgcgcccg gcacctc
//
LOCUS       DATMFS010000078         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_328649, whole genome shotgun sequence.
ACCESSION   DATMFS010000078 DATMFS010000000
VERSION     DATMFS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328649"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VNQ33_01445"
     CDS             complement(<1..748)
                     /locus_tag="VNQ33_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ96788.1"
                     /translation="MQFIDLAAQQRRIREAVDGAVGRVLDHGRYIMGPEVAALEADLA
                     AFVGGGAVVSCASGTDALVMALLVRGAGHGQAVFVPSFTFAATAEAVALLGATPVFVD
                     IDPVTFNLDPASLDAAVRGLDSGLRAAGVIPVDLFGLPADYDAIDAVCAEHGLWVIAD
                     AAQGFGGAIGDRRVGRFAPLTTTSFFPAKPLGCYGDGGAVVALDPDDAAVLRSVRVHG
                     SGSDKYDNVRLGINGRLDTLQAAVLLAKMEI"
     gene            complement(750..1337)
                     /locus_tag="VNQ33_01450"
     CDS             complement(750..1337)
                     /locus_tag="VNQ33_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWJ96789.1"
                     /translation="MSEEDVFVHESAYIDDGAELGAGTKVWHFSHVLPDTRIGAGCTL
                     GQNVVVGPGVVVGDRCRIQNNVSVYAGVTLEDGVFCGPSCVFTNVYNPRAEVSRKDEF
                     RATVVRRGATIGANATIVCGNEIGAYAFVGAGAVVNRPVRAHALVVGAPARQIGWMSH
                     DGERLGDDLTCPRSGRRYQEVGGELHEIVDLGQEA"
     gene            complement(1334..>2262)
                     /locus_tag="VNQ33_01455"
     CDS             complement(1334..>2262)
                     /locus_tag="VNQ33_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ96790.1"
                     /translation="IGSLAAVVDPVPSVAEAHAATYGVAARTFDEVLADATVDGVVIA
                     APAADHARLAEAALAAGKHVFVEKPLALTVGDAERVVAAAAAADRVVMVGHLLQYHPA
                     FLALRDLVDAGRLGELRYLYSNRLNLGQFRQEENSLWSFAPHDLSMLLSLVGEEPDRV
                     WATGAAYLTDGIADVTTTHLAFPGGPHAHVFVSWLHPFKEQRLVVVGSEAMAVFDDLN
                     DWPDKLVLFEHGVDWSDGRPRPKKADGAPVPLDQTEPLRVECEHFLACIQTGATPRTD
                     GLEGLRVLRVLAQAQASLGSIGSGFVQGGGGA"
BASE COUNT          351 a          817 c          799 g          295 t
ORIGIN      
        1 agatttccat cttcgcgagc aacaccgccg cttgcagcgt gtcgagccgg ccgttgatgc
       61 cgaggcgcac gttgtcgtac ttgtccgagc ccgaaccgtg cacgcgcacc gaccggagca
      121 cggcggcgtc atcggggtcc agcgccacga cggcgccgcc gtcgccgtag cagccgagcg
      181 gtttggccgg gaagaacgac gtcgtggtca gcggagcgaa ccggccgacc ctgcggtcgc
      241 cgatcgcacc gccgaagccc tgggcggcgt cagcgatgac ccacagcccg tgctcagcgc
      301 acacggcatc gatggcgtcg tagtcggcgg ggaggccgaa gaggtcgacg gggatcacgc
      361 cggcagcgcg aaggcccgag tcgaggcccc ggaccgcggc gtcgaggctg gccgggtcga
      421 ggttgaaggt caccggatcg atgtcgacga acacgggcgt ggcaccgagg agggcgacgg
      481 cctccgccgt cgccgcgaac gtgaaggacg gcacgaagac cgcctggccg tggcccgcgc
      541 ctcgcacgag gagggccatc accagggcat cggtgccgct ggcgcaggag accaccgccc
      601 ctccacccac gaacgcggcg aggtccgcct ccagggccgc cacctcgggc cccatgatgt
      661 agcgcccgtg gtcgagcacg cggccgacgg cgccgtcgac ggcctcgcgg atccgacgct
      721 gctgcgcagc caggtcgatg aactgcatgt caggcctcct ggccgaggtc gacgatctcg
      781 tgcagctcgc ccccgacctc ctggtagcgc cttccgctgc ggggacacgt taggtcgtct
      841 ccgagccgct ccccgtcgtg gctcatccac ccgatctgac gagcgggagc accgacgacg
      901 agcgcatggg cgcgcaccgg ccggttgacg accgcccccg cgccgacgaa cgcgtacgcg
      961 ccgatctcgt tgccgcagac gatggtggcg ttggcaccga tggtggcgcc ccgccgcacg
     1021 accgtggcac ggaactcgtc tttgcgagac acctcggcgc gggggttgta gacgttcgtg
     1081 aacacgcagg acggcccgca gaacacgccg tcttcgaggg tcacgccggc gtagacgctc
     1141 acgttgttct ggatccggca acggtcgccc accaccacgc cgggtccgac caccacgttc
     1201 tggccgagcg tgcacccggc gccgatgcgg gtgtcgggca gcacgtggga gaagtgccag
     1261 accttggtcc cggcaccgag ctccgcccca tcgtcgatgt acgcggactc gtggacgaag
     1321 acgtcctcct cgctcacgct cccccaccgc cttgcacgaa cccggacccg atcgacccca
     1381 gcgacgcctg ggcctgggcg agcacccgga ggacccggag gccttcgagg ccgtcggtgc
     1441 ggggcgtcgc accggtctgg atgcacgcga ggaagtgctc gcactcgacg cggagcggct
     1501 cggtctggtc caggggcacg ggtgcaccat cggccttctt cggccgcggc cggccgtcgg
     1561 accagtcgac gccgtgctcg aagaggacga gcttgtcggg ccagtcgttc aagtcgtcga
     1621 agacggccat ggcctcgctg ccgaccacca ccaggcgctg ctccttgaac gggtgcagcc
     1681 acgacacgaa cacgtgggcg tgcggcccgc ccgggaacgc caggtgggtg gtcgtgacgt
     1741 cggcgatgcc gtcggtcagg taggccgcac cggtggccca gacccggtcg ggttcttcgc
     1801 ccaccaggct gagcagcatc gacaggtcgt gcggggcgaa gctccagagc gagttctcct
     1861 cctggcggaa ctgcccgagg ttcaaccggt tggagtacag gtagcggagc tcgccgaggc
     1921 gacccgcatc caccaggtcg cgcagtgcca agaacgcggg gtggtactgg agcaggtggc
     1981 ccaccatcac gacgcgatcg gccgccgccg ccgcagccac cacccgctcg gcatcgccca
     2041 ccgtgagggc cagcggcttc tccacgaaca cgtgcttgcc ggccgccagc gcagcctcgg
     2101 cgagtcgggc gtggtcggct gccggcgcgg cgatcaccac gccgtcgacg gtggcgtcgg
     2161 ccaggacctc gtcgaacgtg cgcgccgcca cgccgtaggt ggcggcgtgg gcctcggcaa
     2221 cgctcggcac cgggtcgacc acggccgcca acgacccgat cg
//
LOCUS       DATMFS010000079         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_72706, whole genome shotgun sequence.
ACCESSION   DATMFS010000079 DATMFS010000000
VERSION     DATMFS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72706"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VNQ33_01460"
     CDS             <1..1011
                     /locus_tag="VNQ33_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HWJ96791.1"
                     /translation="DADEPMDWDRLRSAVHTAVRFLDRVIDINYYPTEAAGRSNEGWR
                     PVGLGVMGLQDVFFARRLAFDAPEALALSTRIAEEIYLAALDASVGLAAEAGAPHPRY
                     AETWAARGKLQPDLWGVETTDPRWTPLRERIAEHGLRNSLLIAIAPTATIASIAGCYE
                     CIEPAVSNLFKRETLSGEFLQINTCLVRDLQARGLWDDRMIDRLRAAGGSIQDLDDIP
                     DDLKAVYRTAWEIPQRSLIDLAAARGPFLDQSQSLNLFLESPTIGKLSSMYAYAWKQG
                     LKTTYYLRSRPATRIASATGGGGGAATRPTPGPGASAPAPADDEAVTCSLEDPESCEA
                     CQ"
     gene            1008..2090
                     /locus_tag="VNQ33_01465"
     CDS             1008..2090
                     /locus_tag="VNQ33_01465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWJ96792.1"
                     /translation="MTAVVNPAQPLDRRERPTAAPTPASKRGNILDPGFELTLRPMRY
                     PAFYDMYRDAIRNTWTVEEVDFSDDVGDLARRLDPAERHLIQRLVAFFATGDSIVANN
                     LVLNLYQHINAPEARLYLSRQLYEEALHVQFYLTLLDTYLPDVDEREAAFAAVETIPS
                     IRAKADFCHTWMGALDGVDRLETKEQRRQFLLNLICFATCVEGLFFFAAFAYVYFFRS
                     KGLLNGLASGTNWVFRDESMHMKFALSVVETVKAEEPDLFDAELTRQVTEMLEEAVEA
                     ELQFAEDLLSGGVAGLSVADTRQYLQYVADQRLDNLEMPARFGAENPFDFMMLQDVQE
                     LSNFFERRVSAYQVGITGDVGFDEDF"
     regulatory      2316..2411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2453..3352
                     /locus_tag="VNQ33_01470"
     CDS             2453..3352
                     /locus_tag="VNQ33_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ96793.1"
                     /translation="MDQPTDPVPPTPEALNLKQVARLLDVHYMTAYRYVRQGRLPARQ
                     VGTAWRVERRDVDALLAVPSPEPGSARAPADWVRRLADHLVLGDEIESWRVVGDALNA
                     GHDVASIHLEVIAAAIGRVSSQVADGEHSAATERIAVATSSRLLARLGGQFPHRGRKR
                     GTVVLAAPPGEHHGVPLLLVANLIRHAGYRVVELGTDTPAADVIDAVAALPTVVAVGL
                     GVTTVDRLDAARELVGAVRAAHPSVPVLLGGQAVRNIDIAKLAGATAWSAGPDLIDQL
                     HVLARRTGRPRGVRSQEREAVNR"
     gene            complement(3349..3684)
                     /locus_tag="VNQ33_01475"
     CDS             complement(3349..3684)
                     /locus_tag="VNQ33_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96794.1"
                     /translation="MPADRLSVIVCRGCCCGSEQKHPDVDHDAQVEALRASLPASGRL
                     WTVDCLGPCERSNVVVVRTRAAGRRWFGGVLATAATGALADWIRDGAVGAPPPLLAPL
                     ALSPDEAGS"
     gene            complement(3686..>4276)
                     /locus_tag="VNQ33_01480"
     CDS             complement(3686..>4276)
                     /locus_tag="VNQ33_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ96795.1"
                     /translation="ITMDGTAVDGTPSRDLGRRRSFVSQDQPADLELTVLEVALIGRT
                     PHKGRIEPDRAVDRDIARASLEQVEMHPLRHRTVPTLSGGERQRTFIARALAQQAPVL
                     LLDEPTNHLDLRHQHKLLADLRSAAATVVASLHDPQLALAYADQAVVLDHGRVVGAGT
                     PAEVLTEPVLRDVWGVRATVDRTGSGPGRITISGVA"
BASE COUNT          614 a         1607 c         1474 g          581 t
ORIGIN      
        1 gatgccgacg agccgatgga ctgggatcgc ctgcgctcgg ccgtgcacac ggcggtccgg
       61 ttcctcgacc gggtgatcga catcaactac tacccgacgg aggccgccgg ccggtcgaac
      121 gagggctggc gtccggtggg cctcggcgtg atggggctgc aggacgtgtt cttcgcccgc
      181 cgcctggcct tcgacgcccc cgaggcgctg gcgctgtcga cccgcatcgc cgaggagatc
      241 tacctggctg ccctcgacgc gtcggtcggc ctcgcggccg aggccggggc accgcacccc
      301 cgctacgcgg agacgtgggc cgcccgcggc aagctccagc ccgacctgtg gggcgtcgag
      361 accaccgacc cccgatggac accgctgcgg gagcgcatcg ccgagcacgg cctgcgcaac
      421 tcgctgctca tcgccatcgc cccgacggcg accatcgcct cgatcgccgg ctgctacgag
      481 tgcatcgagc cagccgtgtc caacctcttc aagcgggaga cgctctcggg cgagttcctc
      541 cagatcaaca cctgcctggt gcgcgacctc caggcccgcg gcctctggga cgaccggatg
      601 atcgaccggc tgcgcgctgc gggtggctcg atccaggacc tcgacgacat ccccgacgac
      661 ctcaaggccg tctaccggac cgcgtgggag atcccgcagc ggtcgctcat cgacctggcc
      721 gccgcccggg gcccgttcct cgaccagagc cagtcgctca acctgttcct cgagtcgccc
      781 accatcggga agctgtcgtc gatgtacgcc tacgcctgga agcaggggct gaagacgacg
      841 tactacctgc gctcccgccc ggccacgcgc atcgcgtcgg ccaccggcgg gggagggggc
      901 gccgccacgc ggcccacgcc gggaccaggc gcatcggccc cggctcctgc cgacgacgag
      961 gccgtgacct gttccctcga ggaccccgag agctgcgagg cctgccagtg accgccgtcg
     1021 tgaaccccgc ccagccgctc gatcggcgcg agcggccgac ggcggctccc acgcccgcga
     1081 gcaagcgcgg caacatcctc gaccccggct tcgagctgac gctgcggccc atgcggtacc
     1141 cggcgttcta cgacatgtac cgcgacgcca tccgcaacac ctggaccgtc gaggaggtcg
     1201 acttctccga cgacgtcggt gacctggccc gccgactcga tcccgccgag cggcacctga
     1261 tccagcggct ggtcgccttc ttcgccaccg gcgactcgat cgtggccaac aacctggtgc
     1321 tgaacctcta ccagcacatc aacgcccccg aggcccggct ctacctgagc cgccagctct
     1381 acgaggaggc gctgcacgtc cagttctacc tgaccctcct cgacacctac ctccccgacg
     1441 tggacgagcg cgaagccgcg ttcgccgccg tcgagaccat cccgtcgatc cgggccaagg
     1501 ccgacttctg ccacacgtgg atgggcgcgc tcgacggcgt cgaccgcctc gagaccaaag
     1561 agcagcgccg ccagttcctg ctcaacctca tctgcttcgc cacctgcgtc gagggcctgt
     1621 tcttcttcgc cgccttcgcc tacgtctact tcttccggtc caagggcctg ctcaacggcc
     1681 tcgcttccgg gacgaactgg gtcttccgcg acgagtccat gcacatgaag ttcgccctca
     1741 gcgtggtgga gacggtcaag gccgaagagc ccgacctgtt cgacgccgag ctgacccgcc
     1801 aggtcaccga gatgctcgag gaggcggtgg aggccgagct ccagttcgcc gaggacctgc
     1861 tgtcgggagg cgtggccggc ctgtccgtgg ccgatacccg ccagtacctc cagtacgtgg
     1921 ccgaccagcg gctcgacaac ctcgagatgc cggctcggtt cggtgcggag aaccccttcg
     1981 acttcatgat gctccaggac gtccaggagc tctcgaactt ctttgagcgc cgcgtgagcg
     2041 cctaccaggt gggcatcacc ggcgacgtcg gcttcgacga ggacttctga gctcgcggtc
     2101 cccggatcag ggcgccgcca tcggggagag ccggtccgcg gcgtcgcgca gcagcaccga
     2161 cgtcgcccaa ccgggtccgt ctggtcggtg agcgcgaggg gcggccatga ggccaggtgc
     2221 accgggcgtg catccgatcg cggcttcgaa cgcaggagac ccgaagcgac ctggctccag
     2281 ccggggcgcc agatcgacgc cggaagcgcg ccccggtagc ctgcggccca ggcaggacgg
     2341 ggaacaccgg tgggaatccg gggctgtacc cgcaatggtc accctctcgc cgaggggagc
     2401 ccgacacccg caccgctgcc gttctccgac cagacgcggg agcacgtcga ccatggacca
     2461 gccaaccgat ccggttccgc cgacccctga ggcgctcaac ctcaagcagg tcgcccggct
     2521 gctggacgtc cactacatga cggcctaccg gtacgtccgc cagggccggc tgccggcccg
     2581 acaggtcggc accgcgtggc gggtcgaacg ccgagacgtc gatgcgctcc tcgccgtgcc
     2641 gtcgccggaa ccgggatcgg cgagggcccc cgccgactgg gtccggcggc tggccgacca
     2701 cctggtgctc ggcgacgaga tcgagtcgtg gcgcgtcgtc ggcgacgcgc tcaacgccgg
     2761 ccacgacgtc gcctcgatcc acctcgaggt catcgccgcc gccatcgggc gggtcagcag
     2821 ccaggtggcc gacggcgagc actccgcagc gaccgagcgc atcgccgtcg cgacctccag
     2881 ccggctgctc gcccggctcg gcggccagtt cccccaccgg ggacgcaagc ggggcacggt
     2941 cgtcctggcc gccccgcccg gcgagcacca cggcgtcccc ctgctgctcg tcgccaacct
     3001 gatccgccac gccgggtacc gggtcgtcga gctcgggacc gacacgccgg cggccgacgt
     3061 gatcgacgcc gtggcggcgc tgccgacggt ggtggccgtc gggctgggtg tcaccacggt
     3121 ggaccgcctc gatgccgctc gcgagctcgt gggtgccgtc cgggccgcgc acccgtcggt
     3181 gccggtcctg ctcggtggcc aggcggtccg caacatcgac atcgccaagc tcgccggcgc
     3241 caccgcctgg tcggccggtc ccgacctcat cgaccagctc cacgtcctgg cccgcaggac
     3301 cgggcgtcct cgaggtgtcc ggtcgcagga gcgggaagcg gtgaaccgct agctaccggc
     3361 ctcgtcgggc gaaagggcga gcggcgccag gagcggaggc ggcgcgccga ccgctccgtc
     3421 tcggatccag tccgccaggg caccggtggc ggcggtcgcc agcacaccgc cgaaccagcg
     3481 tcggcccgcg gcgcgggtgc ggacgaccac gacgttggag cgttcgcacg ggccgaggca
     3541 gtcgacggtc cagagccgac cggaggccgg gaggctggct cggagcgcct ccacctgggc
     3601 gtcgtggtcg acgtcggggt gcttctgctc cgagccgcag cagcagcccc ggcacacgat
     3661 caccgacagc cggtccgccg gcatgtcagg cgaccccgga gatggtgatg cggccggggc
     3721 cggatccggt ccggtccacg gtggcgcgga cgccccacac gtcgcgcagc accggctcgg
     3781 tgaggacctc ggcgggggtg cccgcaccga ccacgcgccc gtggtcgagc acgacggcct
     3841 gatcggcgta cgccagggcg agctgcgggt cgtgcagcga cgccacgacc gtcgccgccg
     3901 ccgaccgcag atcggcgagc agcttgtgct ggtggcggag gtcgaggtgg ttcgtcggct
     3961 cgtcgagcag caggacggga gcctgctggg ccagggcccg ggcgatgaag gtccgctggc
     4021 gctcgccgcc cgacagcgtc gggaccgtgc ggtggcggag cgggtgcatc tccacctgct
     4081 cgaggctggc tcgggcgatg tcgcggtcca ccgctcgatc cggttcgatc cggcccttgt
     4141 gcggcgtgcg cccgatgagc gcgacctcca gcacggtgag ctccagatcg gcgggctggt
     4201 cctgcgacac gaacgaccgc cgacggccga gatcgcggct gggtgtgccg tcgaccgcgg
     4261 tcccatccat cgtgat
//
LOCUS       DATMFS010000080         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_305252, whole genome shotgun sequence.
ACCESSION   DATMFS010000080 DATMFS010000000
VERSION     DATMFS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305252"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNQ33_01485"
     CDS             complement(<1..489)
                     /locus_tag="VNQ33_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWJ96796.1"
                     /translation="MTGQLRLLTVHAHPDDEASKGASTIGRYVADGVEATLVCCTGGE
                     AGEILNPAMDRPEVHADLAAVRRAELDASAAAIGFHEVVMLGYRDSGMPDSPENADPR
                     CFARAPEDEAVGRLVAVIRRVRPHVILTYGDEQSGYPHPDHLRVHDISIIAWDAAGDP
                     ARF"
     gene            complement(919..1611)
                     /locus_tag="VNQ33_01490"
     CDS             complement(919..1611)
                     /locus_tag="VNQ33_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96797.1"
                     /translation="MASNDSKQKRARQNRAQREAREARTKAAAIPAEERKAKYASGTP
                     AAEPSSKKERASERARRERPVRPGDVPVDLDTLEGGWYSKRVAVPGGRQVLTGMVLTI
                     IITAMTVFVKYPDPHATTEAAKKKLDHTFLEYWGPKGIVLALVPLVAMAVACWFTTSP
                     NRRRVWTVCAFVASIGIVFGIPYAFPVGFLVYAGMRANKIEGPVPGSRAARAVAAREE
                     AAAAAAEADDDE"
     gene            complement(1683..>2729)
                     /locus_tag="VNQ33_01495"
     CDS             complement(1683..>2729)
                     /locus_tag="VNQ33_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWJ96798.1"
                     /translation="ALVERMADAYFGHRHGRLPLFAEAVAVLDELGERHVLGLVTNGN
                     TDAERGGLAGRFDFRLAADDIGRRKPDPRLFLMAAAAAGCRPGELVYVGDHPGNDVAA
                     PQAAGCRGVWINRAGLPVPPGLEPDAVIADLTELPAVLSRFDEEERAATGRVGVGPHP
                     PPWPDDARLDPELLAAGDRRNVVDGFRYWSEEAIVAELDRTRLPVHVAVENWRHDLNI
                     GTVVRNANAFNVAGVHIVGKRRWNRRGAMATDRYLSVTHHPSVDAFAAWAAEAGLPLV
                     GIDNVAEARPLEAGPLPVPCVLVLGQEGPGLSTAMQEACGEIREITQHGSTRSLNAGV
                     ASGIALHWWAQNRH"
BASE COUNT          358 a          970 c         1019 g          382 t
ORIGIN      
        1 gaaccgtgcc ggatcgccgg ccgcatccca ggcgatgatc gagatgtcgt gcacccgcag
       61 gtggtcgggg tgcgggtagc cgctctgctc gtcgccgtag gtgaggatca cgtgggggcg
      121 gacccggcgg atgacggcca ccagccgccc gacggcctcg tcttcaggcg cccgggcgaa
      181 gcagcgcgga tcggcgttct ccggcgagtc gggcatgccg gagtcccggt agcccagcat
      241 cacgacctcg tggaagccga tggcggcggc cgacgcatcg agctccgccc gccgcaccgc
      301 cgcgaggtcg gcgtgcacct cgggccggtc catcgccggg ttgaggatct cgcccgcctc
      361 gcccccggtg cagcacacca acgtcgcctc gaccccgtcg gcgacgtagc ggccgatggt
      421 ggaggcaccc ttcgacgctt cgtcgtcggg gtgggcgtgg acggtgagga ggcggagttg
      481 gccggtcacg gcgcgccacg gtatcggttc ctggcaccgc cccgacgcga acatcccgcc
      541 aggccgccgc gggtggagcg gtgacctggc gggatgccct gtgtgtgtcg accagtgtgg
      601 ggccgctccc gccgcatgtg tggacgggcc cggtggcgag cgggaggacg caaacgtcga
      661 ctccgaagcg catccgcggt ccgtaatggg aacgggtggc ggcgccgcac tgatcggccg
      721 gggtgcctgt cggcgtcagc ccggaggcga acgctccagg tgatcggtga gcctcacggt
      781 gtcttcacac ctcctcacag gacgagagcg ggttctcgtt ccagccccgg gggaccggat
      841 gcaccgtcac cttcctcctc gcgccggggc gcgtcaagag gaacggttgt tcccgctggc
      901 gaccagcgag gatgcgcgct actcgtcgtc gtcggcttcg gcggcggccg cggcggcttc
      961 ctcccgggcc gcgacggccc gcgccgcgcg cgagccgggg accgggccct cgatcttgtt
     1021 cgcccgcatc ccggcgtaga cgaggaagcc gaccgggaag gcgtagggga tgccgaagac
     1081 gatgccgatc gacgcgacga acgcgcagac ggtccagacc cggcgacggt tcggcgatgt
     1141 ggtgaaccag cacgccacgg ccatggcgac gagcggaacc agcgcgagca cgatgccctt
     1201 cggcccccag tactcgagga acgtgtggtc gagcttcttc ttggccgctt cggtcgtggc
     1261 gtgtgggtcc gggtacttca cgaacacggt catcgccgtg atgatgatcg tgagcaccat
     1321 gccggtgagc acctggcggc caccaggaac ggcgacccgc ttgctgtacc agccgccctc
     1381 gagcgtgtcc aggtcgacgg ggacatcgcc cgggcggacg gggcgctcgc ggcgggcccg
     1441 ctcgctcgcc cgctccttct tggaggaggg ctcagcagcc ggggtgcccg aggcgtactt
     1501 ggccttgcgc tcctcggccg ggatcgccgc cgccttggtg cgcgcctcgc gcgcttcgcg
     1561 ctgggcgcgg ttctgacggg cgcgcttttg cttgctgtcg ttgctggcca cggacagacg
     1621 ctacggaacc ggaccgcccc gaggccaagc cgcaccggag gtgcctccgc tcccgggcag
     1681 gttcagtgtc ggttctgggc ccaccagtgg agcgcgatcc ccgatgccac ccccgcgttc
     1741 aacgaccggg tcgagccgtg ctgggtgatc tcgcggatct cgccgcacgc ctcttgcatc
     1801 gccgtcgaca ggcccggccc ctcctgcccc aggaccagca cgcacggcac cgggaggggg
     1861 cccgcctcca gcggccgcgc ctcggcgacg ttgtcgatcc ccaccagcgg cagcccggcc
     1921 tcggccgccc acgctgcgaa cgcgtccacg gatgggtggt gcgtgaccga caggtagcga
     1981 tcggtcgcca tggccccccg ccggttccag cggcgcttgc cgacgatgtg gaccccggcc
     2041 acgttgaagg cgttggcgtt gcggaccacg gtcccgatgt tcaggtcgtg ccgccagttc
     2101 tcgacggcga cgtggaccgg gaggcgggtg cggtcgagct cggcgacgat cgcctcctcg
     2161 ctccagtacc ggaagccgtc gacgacgttg cgtcggtcgc cggcggcgag gagctcgggg
     2221 tcgaggcgcg catcgtcggg ccacggcggc gggtgcggac ccaccccgac ccggcccgtg
     2281 gcggcgcgct cctcctcgtc gaaccgcgac agcaccgcgg ggagctcggt gaggtcggcg
     2341 atgaccgcat cgggctccag gcccggtgga accggcagac cggcccggtt gatccagacg
     2401 ccgcggcacc cggcggcctg gggagcggcg acgtcgttgc ccgggtggtc gccgacgtag
     2461 accagctcgc caggacggca gccggccgcg gcagccgcca tgaggaacag gcgggggtcg
     2521 ggcttgcggc ggccgatgtc gtcggccgcc aggcggaagt cgaagcgtcc ggcgagcccg
     2581 ccgcgctcgg cgtcggtgtt gccgttcgtg accaggccga gcacgtggcg ctcccccagc
     2641 tcgtcgagca ccgccacggc ctcggcgaag agcggcaggc ggccatggcg gtgcccgaag
     2701 taggcgtcgg ccatgcgctc gaccagcgc
//
LOCUS       DATMFS010000081         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_96184, whole genome shotgun sequence.
ACCESSION   DATMFS010000081 DATMFS010000000
VERSION     DATMFS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96184"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VNQ33_01500"
     CDS             complement(<1..300)
                     /locus_tag="VNQ33_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96799.1"
                     /translation="MPMMRRGPLDRYPPEWVLRQANAHGAEGSIEFHTDRPITLYIQG
                     GRAYAAEAGVNLTEADLADRPIADEGGARDQMVALLAGVMTAQSGWYFHDPLGQHP"
     gene            complement(415..1848)
                     /gene="pyk"
                     /locus_tag="VNQ33_01505"
     CDS             complement(415..1848)
                     /gene="pyk"
                     /locus_tag="VNQ33_01505"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWJ96800.1"
                     /translation="MARRTKIIATIGPASDSEAMIKDLAEAGMDVARIGLAHGSLDEA
                     IEKFRRVRLVEASLGRPIGVMIDLPGPKVRAGTMPEDGLPVDQGDIIHLEPGNTGSTA
                     EMIQVDHEGLLTDLHVGDRVAFGDGIVMAEVIDRKTDCLEARITHGGLLQGRPGVHIP
                     SDRLRIASPTPADLHMLDAFITEGIDMVAVSFVRSAHDVRRVGVEPHPRGPLVVAKIE
                     TRAAVDNLDSIIEASGAVMIARGDLGAEMPIEELPHLQKRILQRCITLGRPAITATQM
                     LESMVTAPSPTRAEASDIANAVFDGSSAVMLSGESAIGHDPINAVATMARIAARADDE
                     FDYDAWGARVHEIASTMQPHVDDTVTLGMTNAAWRAALETGASAIICITHTGFTVRSI
                     ARFRPEAKILGFSADPQTLRQLTLSWGATPQHLDVDYQADNVITRVVDQAREHGLIRR
                     GDLVAVLSGSREYPGQATDSLRLIPIR"
BASE COUNT          264 a          666 c          813 g          329 t
ORIGIN      
        1 cgggtgctgg ccgagcgggt cgtggaagta ccagccggac tgggcggtca tgaccccggc
       61 gagcagggcg accatctggt cgcgggcgcc gccctcgtcg gcgatcggcc gatcggcgag
      121 gtcggcttcg gtgaggttca ccccggcctc ggcggcgtac gcccggccgc cttggatgta
      181 gagcgtgatc gggcggtcgg tgtggaactc gatgctgccc tcggctccgt gggcgttggc
      241 ctgccgaagg acccactccg ggggatagcg gtccaggggg ccgcggcgca tcatcggcat
      301 ggtcgatcct cgtggcggtg gggagggatt ctggacgcgt gaggtccggc accgctaccc
      361 gccattcttc ggccagtcgg gcgccgacct ggactggaaa tgtgtccagg ttcgctaccg
      421 gatggggatg agccgcaggg agtcggtggc ctggccgggg tactcccggc tgccggagag
      481 gaccgccacc aggtcacccc ggcggatgag gccgtgctcc ctcgcctggt cgaccacgcg
      541 ggtgatgacg ttgtcagcct ggtagtcgac gtcgaggtgc tgcggggtgg cgccccagct
      601 gagcgtgagc tggcgcagcg tctgcgggtc ggccgagaag ccgaggatct tggcctcggg
      661 gcggaaccgg gcgatggagc ggaccgtgaa gccggtgtgg gtgatgcaga tgatggcgct
      721 ggcgcccgtc tcgagcgcgg cccgccacgc cgcgttcgtc atgccgaggg tgaccgtgtc
      781 gtcgacgtgg ggctgcatgg tggaggcgat ctcgtggacc cgggcccccc atgcgtcgta
      841 gtcgaactcg tcgtcggccc gggcggcgat gcgggccatg gtggccaccg cgttgatggg
      901 gtcgtggccg atggccgact cgcccgacag catcacggcg ctggagccgt cgaacacggc
      961 gttggcgatg tcggaggctt cggcgcgggt gggcgacggc gccgtgacca tggactcgag
     1021 catctgggtg gcggtgatgg ccgggcgccc gagggtgatg cagcgctgga ggatgcgctt
     1081 ctggaggtgc ggcagctctt cgatcggcat ctccgcaccc agatcgccgc gggcgatcat
     1141 gacggcgcca gacgcctcga tgatgctgtc gaggttgtcg acggccgccc gggtctcgat
     1201 cttggcgacg accagcggtc cgcgcggatg gggctcgacc cccactcggc ggacgtcgtg
     1261 cgccgagcgc acgaacgaca ccgccaccat gtcgatgccc tcggtgatga acgcgtcgag
     1321 catgtggagg tcggccgggg tcggcgaggc gatgcgcagg cggtccgacg ggatgtgcac
     1381 gccgggacgg ccctgcagca ggccgccgtg ggtgatgcgg gcctcgaggc agtcggtctt
     1441 gcggtcgatg acctcggcca tcacgatgcc gtcgccgaac gccacccggt cgccgacgtg
     1501 caggtcggtg aggagcccct cgtggtcgac ctggatcatc tcggccgtgg acccggtgtt
     1561 gccgggctcc aggtggatga tgtcgccctg gtccaccggc agcccgtcct cgggcatggt
     1621 gcccgctcgg accttgggcc ccggcaggtc gatcatcacg ccgatgggcc ggccgaggga
     1681 ggcctcgacc aggcgcaccc ggcggaactt ctcgatggcc tcgtcgagcg agccgtgggc
     1741 cagaccgatg cgggccacgt ccatcccggc ctcggcgagg tccttgatca tcgcctcgga
     1801 gtcggaggcg ggaccgatgg tggcgatgat cttggtgcga cgagccatgg gcccagtgtc
     1861 gcagcccgtc cgcccgattc cgcgccgggt tcggtgaccg tccgctggcg cgcgcgtccc
     1921 actcggacgt cggaaggcca gcacgccgtt cccggcgtcc gttcccggcg tcatcgccta
     1981 cgacgttcgg agaggaggtc cggtcgcggc ggcgagcagg tggccgacga ggtcctcgag
     2041 gctcgtgccg gcgttcaggc cgctggtgga cg
//
LOCUS       DATMFS010000082         3960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_258039, whole genome shotgun sequence.
ACCESSION   DATMFS010000082 DATMFS010000000
VERSION     DATMFS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258039"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VNQ33_01510"
     CDS             <1..815
                     /locus_tag="VNQ33_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96801.1"
                     /translation="LEVFMLRRNLNSDFVGGAYVFPGGAVDPEDRHANLEPVCEGRTD
                     TDASRRLGIESGGLAFWVAAIRESFEEAGVLLAYDLDGIVDLDDTASMERWAHHRRQV
                     DTGQLTMVDLCRLEGLRLAVDGMHYFSHWVTPEGAPRRYDTRFFLAAAPLNQTPLHDD
                     HEVIANEWIRPADGIARAESGDLTMMPPTIASLRAISRFDTAAEALDAAREITHVPEV
                     LPRVIEADGGVRIVLPGDPAYDAPQGVPDASLNWMHIADSRKVTGFGATEPA"
     gene            812..1678
                     /locus_tag="VNQ33_01515"
     CDS             812..1678
                     /locus_tag="VNQ33_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ96802.1"
                     /translation="MSTAEAEAARPELIPDVPIALGKLVRRVLCNNPGMMTGPGTNTY
                     LVGQDEIAVIDPGPDDADHLDAIAAAGSGRIRWILCTHTHGDHSPGAAGLKERTGAEV
                     LAFADRDGLVCDRHLTDGDTVEGTEFTLRVIHTPGHAANHICFLLERDRLLFSGDHIM
                     DGSTVVITPLDGDMGEYLASLERLQAWMPRLKAIAPAHGHVIDDPAAKLDDYITHRLE
                     REAQVLAALQGFGAGGAGTAQLVEAIYTDVPELLWPVARFSVWAHLRKLATEGTAGAV
                     DADDPDTTWTAT"
     gene            complement(1734..2141)
                     /locus_tag="VNQ33_01520"
     CDS             complement(1734..2141)
                     /locus_tag="VNQ33_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ96803.1"
                     /translation="MTRPLSDRDYQSLASFRRELRRFLAFSEGEAKTAGLTPAHHQLL
                     LAIRGHIGDPPSISDLADALQLKLHSATELVARAEAKGLVTREHTDPDDARRAVVALT
                     DEGAAKLAQLSAVHREEIRRFRAQLDDILATLD"
     gene            complement(2138..3475)
                     /locus_tag="VNQ33_01525"
     CDS             complement(2138..3475)
                     /locus_tag="VNQ33_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWJ96804.1"
                     /translation="MRALPIPPLHSERTRMAALVLAVGVGGGLVGAAYLTALKAMTEV
                     LGPEQWGEWPHLVVLVLVGVSVTGLIRWLGTPGDVELLVNNIHVDGGRDDVRSLRSLI
                     PVSLLCVGAGGTLGPEAPLVTTTGTLASWLGVRRGLGRHDLRVVAITGMAAGFTVLFA
                     APLGSAVFALEILHRRGLEYYEALLPAVVGALCGYGISAALTVIGVDPIWTFAAPAHL
                     VPIDFALAIGAGVLGALVAIVFTYLAIGLRAVADRLPSGTQPAIGGAVLGLIAIASPY
                     ALTNGELQINHLSLTGAAALTFLVAAVAKLVAAAVSVATGWRGGFIIPLFFVGFALAQ
                     AADGHLGGGSTWVLAAGLMVACNVGVTKTPLGSTLVVTEMSGAAVLPTTLIAALVSLA
                     LTSQISLIHTQRRRFTALPVSGEAEPPDVPDETDSADLSGRPAGAAPRTEEQR"
     gene            3615..>3960
                     /locus_tag="VNQ33_01530"
     CDS             3615..>3960
                     /locus_tag="VNQ33_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ96805.1"
                     /translation="MADYQLFIDGEYVDAQSGETFDTTDPATGQVIGSIAKAGREDSV
                     KAIEAARTAFDEGPWPKMSGPERAAKINQIADAIEAKADTFSEMEARDGGGTIKKAMF
                     ADVPGAVGAFRWF"
BASE COUNT          612 a         1420 c         1429 g          499 t
ORIGIN      
        1 gcctcgaggt cttcatgctc cggcgcaacc tcaactccga cttcgtgggc ggcgcctacg
       61 tcttccccgg cggcgcggtc gatcccgagg accggcacgc caacctggag ccggtctgcg
      121 aggggcggac cgacactgac gcatcccgcc ggctgggcat cgagtccggt ggtctggcgt
      181 tctgggtcgc cgccatccgg gagagcttcg aagaggccgg cgtgctgctg gcctacgacc
      241 tcgacggcat cgtcgacctc gacgacacgg cgtcgatgga gcggtgggcc caccaccgcc
      301 gacaggtcga caccgggcag ctgacgatgg tggacctgtg ccggctcgag ggcctgcgcc
      361 tcgccgtcga cggcatgcac tacttcagcc actgggttac gcctgaaggc gcgccccgcc
      421 ggtacgacac ccggttcttc ctcgccgccg cgccgctcaa ccagacgccg ctgcacgacg
      481 accacgaggt catcgccaac gagtggatcc ggccagccga cggcatcgcc cgcgccgagt
      541 ccggggacct cacgatgatg ccgcccacca tcgccagcct ccgggccatc tcccggttcg
      601 acacggcggc cgaggcgctg gacgcggctc gggagatcac gcacgtcccc gaggtcctgc
      661 cccgggtgat cgaggcggat ggcggcgtgc ggatcgtcct gcccggcgat ccggcctacg
      721 acgcgccgca gggcgtaccg gacgcatccc tcaactggat gcacatcgcc gactcccgga
      781 aggtcaccgg gttcggcgcc acggagccgg cgtgagcacg gccgaggccg aggccgcccg
      841 cccggagctg atccccgacg tccccatcgc cctgggcaag ctcgtccgcc gggtgctctg
      901 caacaacccc gggatgatga cgggcccggg caccaacacg tacctggtcg ggcaggacga
      961 gatcgccgtc atcgaccccg ggcccgacga cgccgaccac ctcgatgcca tcgccgcggc
     1021 gggcagcggc cggatccgct ggatcctctg cacgcacacc cacggcgacc attcgccggg
     1081 cgctgccggg ctcaaggagc gcacgggcgc cgaggtgctg gcgttcgccg accgcgacgg
     1141 gctggtgtgc gaccgccacc tcaccgatgg cgacaccgtc gaaggcaccg agttcacgct
     1201 gcgggtcatc cacacgccgg gtcacgccgc caaccacatc tgcttcctgc tcgagcggga
     1261 ccggctgctg ttcagcggcg accacatcat ggacggctcc accgtcgtca tcaccccgct
     1321 cgacggcgac atgggcgagt acctcgcctc gctggagcgg ctgcaggcgt ggatgccccg
     1381 gctcaaggcc atcgcccccg cccacggcca cgtcatcgac gacccggcgg ccaagctgga
     1441 cgactacatc acccaccggc tcgagcggga ggcccaggtg ctggccgccc tccagggctt
     1501 cggggccggg ggcgccggca ccgcccagct ggtggaggcg atctacaccg acgtgcccga
     1561 gctcctctgg cccgtcgccc gcttcagcgt gtgggcccac ctccgcaagc tcgccaccga
     1621 gggcaccgcg ggcgccgtcg acgccgacga ccccgacacg acctggaccg ctacctgacc
     1681 ggatctccgt gggctctggt cgaggtcggc tggccgtgag ggcccgtcga tgatcaatcg
     1741 agcgtggcca agatgtcgtc gagctgggcg cggaagcggc ggatctcctc gcggtggacg
     1801 gcggagagct gtgcgagctt ggcggcgccc tcgtcggtga gggcgacgac ggcccgccgg
     1861 gcatcgtcgg ggtcggtgtg ctcgcgggtg accaggccct tggcctcggc ccgggccacc
     1921 agctcggtgg ccgagtggag cttgagctgg agggcgtcgg cgaggtcgct gatcgacggc
     1981 gggtcgccga tgtggccgcg gatggcgagc aggagctggt ggtgggcggg ggtcaatccg
     2041 gccgtcttcg cctcgccctc cgagaaggcg aggaaccggc gcagctcccg ccggaacgac
     2101 gccagcgact ggtagtcgcg gtccgacagc ggtctggtca tcgctgctcc tccgttcggg
     2161 gggcggcacc ggcggggcgg ccggacaggt cggcgctgtc cgtctcgtcg ggcacgtcgg
     2221 ggggctccgc ctcgccgctg acgggaagag ccgtgaagcg ccggcgctgg gtgtggatca
     2281 ggctgatctg cgacgtgagc gccaagctga ccagggcagc gatcagggtc gtgggcaaca
     2341 cggcggcacc gctcatctcg gtgacgacga gcgtcgaccc gagcggggtc ttggtgacgc
     2401 ccacgttgca ggccaccatc agcccggccg cgaggaccca ggtgctgccg cctccgaggt
     2461 ggccgtcggc cgcctgggcg agggcgaacc cgacgaagaa cagcgggatg atgaacccgc
     2521 cccgccatcc ggtggccacc gatacggcgg cggccaccag cttcgccacc gccgcgacca
     2581 ggaacgtcag ggcggctgcg ccggtcaggc tgaggtggtt gatctgcagc tcgccgttgg
     2641 tgagcgcgta gggggaggcg atggcgatca ggcccaagac ggccccgccg atcgccggct
     2701 gggtgccgga ggggaggcga tcggcgacgg cgcgcaggcc gatcgccagg taggtgaaga
     2761 cgatggcgac cagagcaccg aggacgccgg ctccgatggc cagcgcgaag tcgatcggca
     2821 ccaggtgcgc cggagccgcg aaggtccaga tcggatcgac gccgatcacg gtgagcgccg
     2881 ccgagatccc gtagccgcag agcgcgccca ccacggcagg gagcagggcc tcgtagtact
     2941 ccagccctcg ccggtgcagg atctccaggg cgaacacggc agagccgagc ggggcggcga
     3001 acagcacggt gaaccccgcg gccatgccgg tgatggcgac gactcgaagg tcgtggcgac
     3061 ccagcccacg ccggaccccg agccacgatg ccagcgtgcc cgtggtggtg acgagcggcg
     3121 cctcggggcc gagcgtgccg cccgctccga cgcacagcag cgagaccggg atgagcgacc
     3181 ggaggctgcg gacgtcgtcg cgtccgccgt cgacgtggat gttgttgacg aggagctcga
     3241 cgtcaccggg cgtccccagc caccggatga gccccgtcac cgagacgccg acgagcacca
     3301 ggaccacgag gtgcggccac tcgccccact gctcgggacc gagcacctcg gtcatcgcct
     3361 tcagcgccgt caggtaggcg gcgccgacga gcccgccgcc gacgcccacc gccagcacga
     3421 gcgcggccat ccgcgtccgt tcgctgtgga gcggtgggat ggggagggcg cgcacgagat
     3481 agatcgtagt gcgatctatc tggccgagcc aagcgggacg cggtggccgc aggggcgcgg
     3541 tagcgtcctt gaccgtcggg tcaaggggac ccgcgtgcgc cctccgcacc acatccacca
     3601 aggggaacgg caccatggcc gactaccagc tcttcatcga cggcgagtac gtcgatgccc
     3661 agtcggggga gaccttcgac accaccgatc cggccaccgg gcaggtcatc ggcagcatcg
     3721 ccaaggccgg tcgcgaggac tcggtcaagg ccatcgaggc cgcccgcacg gcgttcgacg
     3781 agggcccctg gcccaagatg agcggccctg agcgcgccgc caagatcaac cagatcgccg
     3841 atgccatcga ggccaaggcc gacacgttca gcgagatgga ggcccgcgac ggcggcggca
     3901 ccatcaagaa ggcgatgttc gccgacgtgc ccggtgccgt cggtgcgttc cgctggttcg
//
LOCUS       DATMFS010000083         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_189519, whole genome shotgun sequence.
ACCESSION   DATMFS010000083 DATMFS010000000
VERSION     DATMFS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189519"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1073..1942
                     /locus_tag="VNQ33_01535"
     CDS             1073..1942
                     /locus_tag="VNQ33_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96806.1"
                     /translation="MIGGVAAYARDMVDGDLPAATKDFDAWIARRILSPAAPLSREVD
                     LLLAEDPTTAQARKASLYHAALAAVALGRRTPGRMADYVNISGQRLDPILRGLVEAQF
                     LERLVDPIRDNRPTYHPGDPIIRFHYAVIRPNHARLGRHGADLGSMWRSLGSTFSSNV
                     VGPGFELIARHWLMHYADLSSVLDGPSHVGSTVMSVDGTEYEVDLVVARDDGDVPAER
                     SIAALGEVKSGEELDRSHLARLESVRTGLGPRAADAALYLVGRRFTDVLSATAAQRSD
                     VELVDLDRLYYGS"
     gene            complement(2001..>3196)
                     /locus_tag="VNQ33_01540"
     CDS             complement(2001..>3196)
                     /locus_tag="VNQ33_01540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWJ96807.1"
                     /translation="LEGADLYVLMLLSAAGGLVMTGANDLLVLFLGLEILSIAVYVLA
                     GMHIRRARSGEAALKYFVLGAFSSAFFLYGIAMVYGATGSTNFTKISAFLAANILTNN
                     VMLLAGFALLLVGLGFKVAAVPFHAWTPDVYEGSPSPVVAYMASGVKVAGFAGMIRVF
                     TSTFSSYAADWQPMVYVLAVATLVVGSLFAVSQTNVKRMLAYSSISHAGFILLGIQAA
                     STQGTRAALFYLATYTFMAAGSFGVVTVVGRAGDNAHDLSDYDGLAKRSPLLAFAFLV
                     FLLGQAGVPFTSGFLAKFGVIGAAVEARSFWLALIAMLTSVVSAFVYLRIVLAMYDRE
                     VEVSGPKLRVPVGARIALLIAVLVTIGVGLVPEPLSHATRTAVALTHPAPAPAAAPIE
                     VSAGS"
BASE COUNT          549 a         1070 c         1110 g          467 t
ORIGIN      
        1 cgcaccccga cctgccggcc gagacagcgg gcgaggacgg cagcagagct gtcctcgcca
       61 cgcagcgcaa ggaaaggata accgtccatc ccttccttga ggagccggct cgtgaaggtt
      121 gccaccgctc cgccagccgg acgaagccgg tgtgggaccc agggatcgaa ctgggccccg
      181 cggcgtggcg tcggcgccgc cccacccggt gtccgccagc ctgccgaccg ccacggacag
      241 caccttcgcg gccctcaccg cctaccgggg atacccctgc cgacatcgcg gagcgcctgg
      301 cgaacgcatc gttcgccagg catcacgagc ggccgcggct ggtcgccgac ctccagacca
      361 cccgagacaa gtggtcggcc gcggcgccga tgacaccgaa cgtggtcgga agcgacgttc
      421 gcaccaccgt tctcattcag gtgtacattt acaatgtatg gatgaacgaa tagccgccac
      481 gctgtcgccc gattctgtcg ggcgtcgcgc cgagtgggac caactcaacg cattctcgct
      541 gtctcctgtc agccactcga ccctcggcat cgtctgggga cgccgacgca tcggcaagtc
      601 gttcctcctc agccagctcg gtgaccagca gggcttgtac tacgaggcgg tccggggatc
      661 agcgagcgag gcgctccgag cgttgggagc cgtggcgggt gacgctgtcg gagcgccgtc
      721 tccgctcgac ctgcgcgact gggagcacgc cgtctcgaca ctgctgcaac tgggccagcg
      781 gcagcccttc accgtcgtgc tcgatgagta cccgtacctt cgggaggagt ccccagagct
      841 cgactcgatc atccagcggg cgttcggccc gaagagcgcg ctgcgaacgc agaaccgatg
      901 ccgtctgatc ttgtgcggct ctgcgatgtc ggtgatgtcg gagctgctca gcggcacggc
      961 gccactccga ggccgagcga gcttggacct gagggtctcc cccttcgact tccgtgatgc
     1021 gctggcgctc cacggcacag ccgatctgga cctcgccgtc cgcctgtact cggtgatcgg
     1081 tggcgtggcc gcgtatgcgc gcgacatggt ggacggcgac ctgcctgcgg cgacgaagga
     1141 cttcgatgcc tggatcgcgc gccggatcct ctctcccgcg gcacccctgt cgagggaggt
     1201 cgatctcctc ttggcagagg atcccacgac ggcacaggcc cgcaaggcga gcttgtacca
     1261 cgctgcgctc gccgccgtcg ccctgggacg gcgtacaccc ggacggatgg ccgactacgt
     1321 gaacatctcg ggccagcgac tcgacccgat cctccggggt ctggtcgaag ctcagttcct
     1381 cgagcgcctg gtcgatccga tccgcgacaa tcggccgacc tatcatcccg gcgacccgat
     1441 catccggttc cactatgcgg tcatccgtcc caaccatgcc cggctggggc ggcacggagc
     1501 ggacctcgga tcgatgtggc gctcgctcgg ctcgacgttc agctccaatg tcgtcggccc
     1561 tgggttcgag ctcatcgccc gccattggct gatgcactac gccgaccttt catcggtgct
     1621 cgacggaccg tcgcacgtcg gttcgaccgt gatgtcggtc gatgggaccg agtacgaggt
     1681 cgacctcgtc gtcgcccgcg atgacggcga tgtgccagcc gagcgctcga tcgcagcgct
     1741 cggtgaggtg aagtccggtg aggaactgga ccgcagccac cttgcacgcc tcgaatcggt
     1801 gaggacgggg ctcgggccac gagccgccga cgctgccctt tacctggtcg gtcgacggtt
     1861 caccgatgtg ttgtcggcaa ccgcagcaca acgctccgac gtggaactgg tcgatctcga
     1921 ccgcctctac tacggcagct gaccaaagag cacgggagcg accaccccga acgatccacc
     1981 gcaggcatct gcagtgcagg tcagctgccg gcggagacct cgatcggggc ggctgcaggt
     2041 gcaggcgcgg ggtgggtgag cgcgaccgcg gtgcgggtgg cgtggctgag gggctcgggg
     2101 accaggccca cgccgatggt gaccaggacg gcgatgagga gggcgatgcg ggcgccgacc
     2161 gggacgcgga gcttcgggcc ggagacctcg acctcgcggt cgtacatggc gagcacgatg
     2221 cgcaggtaga cgaaggcgga gacgaccgac gtgagcatgg cgatgagcgc cagccagaac
     2281 gaccgggcct ccaccgccgc gccgatgacg ccgaacttgg ccaggaagcc ggaggtgaac
     2341 ggcacgccgg cctgcccgag caggaacacc aagaaggcga aggcgagcag cggcgagcgc
     2401 ttggccaggc cgtcgtagtc ggacaggtcg tgggcgttgt cgcccgcccg gccgacgacg
     2461 gtgaccacac cgaagctgcc cgccgccatg aacgtgtagg tggccaggta gaagagcgcg
     2521 gcgcgggtgc cctgggtgct ggccgcctgg atgccgagga ggatgaagcc ggcgtggctg
     2581 atcgacgagt acgccagcat ccgcttgacg ttggtctggc tcaccgcgaa cagggagccg
     2641 accacgagcg tggccaccgc cagcacgtag accatcggct gccagtcggc ggcgtagctg
     2701 gagaaggtgg aggtgaagac ccggatcatc ccggcgaagc cggcgacctt gacgcccgac
     2761 gccatgtagg cgaccaccgg gctgggcgag ccctcgtaga cgtcgggggt ccaggcgtgg
     2821 aacggcaccg ccgccacctt gaacccgagg cccaccagga gcagggcgaa cccggccagc
     2881 agcatcacgt tgttcgtgag gatgttggcg gcgaggaagg cgctgatctt ggtgaagttc
     2941 gtgctccccg tggcgccgta gaccatggcg atgccgtaga ggaagaaggc cgacgagaag
     3001 gcgcccagca cgaagtactt gagggcggcc tcgccggatc gggcccggcg gatgtgcatg
     3061 ccagccagca cgtagacggc gatcgagagg atctccaggc cgaggaacag caccaggagg
     3121 tcgttggcgc cggtcatcac caggccgccc gcggcagaca gcagcatcag gacgtagagg
     3181 tcggcgccct cgagct
//
LOCUS       DATMFS010000084         3362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_165781, whole genome shotgun sequence.
ACCESSION   DATMFS010000084 DATMFS010000000
VERSION     DATMFS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165781"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VNQ33_01545"
     CDS             <1..449
                     /locus_tag="VNQ33_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ96808.1"
                     /translation="LPQRASASNQVVRLAFGDRPSKRAVEIVGEMARSIPPEALLPSL
                     SGLIEHDAREALKATNTPSLVIVGTRDLLTPVPSARHLAHLLPDSELVVLPRAGHQLM
                     QERPDELAELIDAFVARLDGTEPTVAAAVAASEGSVEPDQVEDGGG"
     gene            661..1158
                     /locus_tag="VNQ33_01550"
     CDS             661..1158
                     /locus_tag="VNQ33_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96809.1"
                     /translation="MNELPSSELDAPATKEFVRAEVSASSAELGASIAELRAELVRST
                     GEVRSELGTSIGEVRAELGTSIGEVRAELGASIAELRAELAASIGEVRSELGTSIGEV
                     RAELGTSIGEVREEIGAVRGDVGALRTEMHVLARSQTQWFCGLLVGAMSVVVAMNAWV
                     VVTLR"
     gene            complement(1145..1501)
                     /locus_tag="VNQ33_01555"
     CDS             complement(1145..1501)
                     /locus_tag="VNQ33_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWJ96810.1"
                     /translation="MCSPPGRRLPQLLVDGRAVAPVEVAASRRARGRGLLGRARLDGA
                     LLLAPARSVHTFGMRFPIDVALCTQDLQVRAVTSLAPGRLTVPRWGVRAVVEAEAGRF
                     EEWGIRPGTSLGLNGA"
     gene            1680..2192
                     /gene="tsaE"
                     /locus_tag="VNQ33_01560"
     CDS             1680..2192
                     /gene="tsaE"
                     /locus_tag="VNQ33_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWJ96811.1"
                     /translation="MSRWPVVVATTSAAHTQDLAAALAATAVPGDLVLLAGEMGAGKT
                     AFAQGFARGLGITDQVTSPTFTIVQEYTGGRLNMHHLDVYRLESLREVADLGLGEMLD
                     EDAVMLVEWGDAVLPALPDQYLELRITFGTDEDARRIELRGVGGSWPARARAIAEAVA
                     AWRVEAGPAC"
     gene            2186..2884
                     /gene="tsaB"
                     /locus_tag="VNQ33_01565"
     CDS             2186..2884
                     /gene="tsaB"
                     /locus_tag="VNQ33_01565"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWJ96812.1"
                     /translation="MLILGLESATAEVGCAIGGHEGVLASSHSARGKRHAENLTPAIE
                     FVCRQARVELSEISCVAVDNGPGLFTGLRVGVASGKAIAQALRVPMISVSSLDLLAFP
                     LRFTDRRIATVIDARRNEVFWAFYRQVPGGIQRISEPAVGGADDLAAELQAQPGDTLL
                     AGDGAVRYAEALTLRPGIEVAGGDHAYPSARSLVQLAHAKALREDWVRPEQIELLYLR
                     KPDAEINWSVREGA"
     gene            2938..>3362
                     /gene="rimI"
                     /locus_tag="VNQ33_01570"
     CDS             2938..>3362
                     /gene="rimI"
                     /locus_tag="VNQ33_01570"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWJ96813.1"
                     /translation="MRRRHLPDVLAIEAQVYPRPWSSRLFEDELERSGRVYLVARLGP
                     TVVGYAGLLMIADDGHVATIAVDPARHRHGIARCLLVELVRQAMELGANQLTLEVRVS
                     NRGAQDLYRSLGFAPGGARKAYYSDNGEDALVMWAHDIR"
BASE COUNT          440 a         1189 c         1238 g          495 t
ORIGIN      
        1 cgctcccgca gcgggcctcg gcgtcgaacc aggtcgtgcg cctcgcgttc ggcgatcggc
       61 cgtccaagcg ggcggtcgag atcgtgggcg agatggcccg gtcgatcccg cccgaggcgc
      121 tgctgccctc gctcagcggc ctcatcgagc acgacgcccg tgaggccctc aaggcgacga
      181 acacgccctc gctggtgatc gtcggcaccc gcgacctgct gacgccggtg ccctccgccc
      241 gccatctcgc ccacctgctc ccggactcgg agctggtggt gctgccgcgc gccggccacc
      301 agctgatgca ggagcggccc gacgagctgg ccgagctgat cgacgcgttc gtcgcccgcc
      361 tcgacggcac cgagcccacg gtcgccgcgg ccgtggccgc ctccgaaggc tcggtcgagc
      421 cggaccaggt cgaggacggg ggaggctgac cagacgaatg ggcttcgttc cctggtggtt
      481 cctcagcatt ttcatggttg ttcatcgaac gacgggctgc tagcctgagc atcgccttcc
      541 caccaccccc tccatcgttc gcctgttctt ggagatgtcg tggcgttgtc gcaccagcac
      601 cgatcgtcgc tgtacctatc gctcgcaccc atcgttggct ccgaagaagc ggaggcactc
      661 ttgaacgagc ttcccagctc cgagctcgac gctccggcca ccaaggagtt cgtccgtgcc
      721 gaggtcagcg cgtcaagcgc ggagctgggt gcctcgatcg ccgagctccg agcggagctg
      781 gtgaggtcga ccggcgaggt gcgctcggag ttggggacgt cgattggcga ggtgcgcgcg
      841 gagctgggga cgtcgattgg cgaggtccgc gcggagctgg gtgcttcgat cgccgagctc
      901 cgagcggagc tggcggcgtc gattggcgag gtgcgctcgg agttggggac gtcgattggc
      961 gaggtgcgcg cggagttggg gacgtcgatc ggcgaggtcc gcgaggagat cggcgcggtg
     1021 cgcggtgacg tgggtgcact gcgtaccgag atgcacgtcc tcgctcggtc gcagacgcag
     1081 tggttctgcg gcttgctcgt gggagcgatg agcgtcgtcg tagccatgaa cgcctgggtc
     1141 gtggtcacgc tccgttgagc ccgaggctcg taccggggcg gatcccccac tcctcgaatc
     1201 gcccggcctc cgcctccacc acagcacgga cgccccagcg gggaacggtg agccgacccg
     1261 gtgcgaggga ggtgacggcc cggacctgca ggtcctgggt gcacaaggcg acgtcgatgg
     1321 ggaaccgcat gccgaacgtg tgcacggatc gagcgggagc gaggagcagg gccccgtcga
     1381 gccgggcgcg gccgagcagg ccgcgccccc tcgcccgacg gcttgcggcg acctcgacgg
     1441 gggccacggc ccggccatcg acgaggagct ggggaagccg tcggccagga ggggagcaca
     1501 cggtgctccg gtgtagcgga ggtcgggagc gctccgcacc ggacgcgcct cgatgcgacc
     1561 catctggcgc tgctcggcca ggttcgtgcg cggtgcgccg gtccgtaaga tcgtgcggtg
     1621 gcctccacct ccctcctcct cgccgcgtct tcggccgggc gcgcccggga ggcggcccgg
     1681 tgagccgctg gccggtggtc gtcgccacca ccagcgcggc ccacacccag gacctcgcgg
     1741 ccgcgctggc tgccaccgcg gtgcctggcg atctggtgct gctcgccggt gagatggggg
     1801 cgggcaagac ggcgttcgcc cagggctttg cccgtgggct cggcatcacc gaccaggtga
     1861 cgagcccgac gttcacgatc gtgcaggagt acacgggcgg gcgcctcaac atgcaccacc
     1921 tggacgtgta ccggctcgag tcgctccggg aggtggccga cctcggcctg ggcgagatgc
     1981 tcgacgagga cgcggtgatg ctcgtggaat ggggcgatgc cgtgctgccc gcgctcccgg
     2041 accagtacct cgagctgcgc atcacgttcg gaaccgacga ggacgcgcgc cgcatcgagc
     2101 tgcgcggcgt cggcgggtcg tggccggcgc gcgctcgggc catcgccgag gccgtcgccg
     2161 cctggcgcgt cgaagcgggg ccggcgtgct gatcctgggc ctcgagagcg ccaccgccga
     2221 ggtgggctgt gccatcggcg gccacgaggg cgtgctggcg tcgtcgcact cggcgcgggg
     2281 caagcgccac gccgagaacc tcacgccggc catcgagttc gtctgccgcc aagcccgcgt
     2341 cgagctgtcg gagatctcct gcgtcgccgt cgacaacggc ccgggcctct tcaccgggct
     2401 gcgcgtcggc gtcgcctcgg gcaaggccat cgcccaggcc ctgcgggtgc cgatgatctc
     2461 ggtgtcgagc ctggacctgc tggccttccc gctccgcttc accgatcgcc gcatcgcgac
     2521 ggtgatcgac gcccgtcgca acgaggtgtt ctgggcgttc taccgccagg tccccggcgg
     2581 catccagcgg atcagcgagc ccgcggtggg cggcgccgac gatctcgccg ccgagctgca
     2641 ggcgcagccc ggcgacacgc tgctcgccgg cgacggcgcg gtgcgctacg ccgaggcgct
     2701 caccctccga ccgggcatcg aggtcgccgg cggggaccac gcctacccgt cggcccggtc
     2761 gctggtgcag ctcgcccacg ccaaggcgct gcgggaggac tgggtgcggc ccgagcagat
     2821 cgagctgctc tacctccgca agcccgacgc cgagatcaac tggtccgtgc gggagggggc
     2881 gtgacagctg cgtcgggcaa ggctggagcg ggtgccggca ccttgaccgt gtcgcccatg
     2941 cgtcgtcggc acctgcccga cgtgttggcc atcgaggccc aggtgtaccc gcgcccgtgg
     3001 tcgtcgcggc tgttcgagga cgagctcgaa cggtcgggcc gggtctacct cgtggcccgg
     3061 ctcggcccga cggtggtcgg ctacgccggc ctgctgatga tcgccgacga cggccacgtc
     3121 gccaccatcg ccgtcgaccc ggcccggcac cgccacggca tcgcccgctg cctcctcgtc
     3181 gagctggtcc gccaggcgat ggagctgggc gccaaccagc tcaccctcga ggtgcgggtg
     3241 tccaaccgcg gcgcccagga cctgtaccgc agcctcgggt tcgctcccgg aggcgcccgc
     3301 aaggcgtact attcggacaa cggcgaggat gccctggtga tgtgggcgca cgacatccgc
     3361 ag
//
LOCUS       DATMFS010000085         4314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_142862, whole genome shotgun sequence.
ACCESSION   DATMFS010000085 DATMFS010000000
VERSION     DATMFS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142862"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VNQ33_01575"
     CDS             <1..827
                     /locus_tag="VNQ33_01575"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWJ96814.1"
                     /translation="IPSFLKRVDLADRGVAWSDYLGRNREAMDDIAERFFPSADDVQE
                     APRTTLVDFDPDAEIHMVTAMLYAHAHAPERQIEERVRQMSAEERLSVVRAYVGERAN
                     RRHRPGRALERIDYRFDVLADYGAFRDLQRHRMLTIEWQPLTPLHGYTRPEAVDLAGE
                     TEAFDRAMERSASLYELLATRFPAEASYAVCLAYKVRFTMQMNAREAMHLLELRSGPQ
                     GHPAYREVAQDMHRLIATEAGHHAVAEMMRYVDHSPEPSLERLDSERRAEARREGR"
     gene            935..1441
                     /locus_tag="VNQ33_01580"
     CDS             935..1441
                     /locus_tag="VNQ33_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96815.1"
                     /translation="MADDELIDDEIDVEEDDELIDEIDDELEGDDLEDDDDVVPDEEL
                     VASVVDDDEDAEEEPPPAPVKGRSGDDDEDDDDDVDPDDVEEDLDTILKDRIAAGADD
                     DDDDDEEAPVDEGPGGDRVAPRAAEEFSCNECFLLVRRSQFSAKRTDCPGGLDGEDCP
                     VKRELGLV"
     gene            1514..2020
                     /locus_tag="VNQ33_01585"
     CDS             1514..2020
                     /locus_tag="VNQ33_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96816.1"
                     /translation="MSDDKNPIEHAVELTVLAPIGLALEAKRLLPTFVERGRQQVNMA
                     KVIGQFAVAHGQTTATQKLTQAQAQAASILAEFGLVGSGEDGAVPDDAPAAPAEPTEP
                     AAPAAPRSSEAAADLPIADYDSLAASQVIPRLAGLSAAELAAVEAYEVGHRGRKTILG
                     KITQLGAE"
     gene            2025..2543
                     /locus_tag="VNQ33_01590"
     CDS             2025..2543
                     /locus_tag="VNQ33_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ96817.1"
                     /translation="MEAARAALLSDVAAITELARTAVGELGLLRGGPVWQLQDARVEP
                     IEDGVAADVALQPDEGLAVVGTIDGAIVGYGVSRLETLADGSVLAEVSDLYVDPGARG
                     IGLGEAMMDLLVAHAEQAGAIGIDALALPGDRATKNFFETFGLKARAIVVHRSLQATE
                     LPQGPGGDDGSA"
     gene            2530..2937
                     /locus_tag="VNQ33_01595"
     CDS             2530..2937
                     /locus_tag="VNQ33_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWJ96818.1"
                     /translation="MEAPELCVSAVVIDDDRLLLVRRGRGRAQGEWAVPGGRVRPGEL
                     LAEAVVRELIEETGIEGVCGNLIGVLELLDDAGPHQVVLGHLVTLMEATDPVAGDDAA
                     EARWVPLSDVAELRLASGLAEFLHDHEVIATIT"
     gene            complement(2953..3729)
                     /locus_tag="VNQ33_01600"
     CDS             complement(2953..3729)
                     /locus_tag="VNQ33_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWJ96819.1"
                     /translation="MTVDYELRGHIAVITINRPDARNAVNTDVAQGIEAAIDQLEEDP
                     NAWVGILTGAQTAKGWIFCAGADLKQMAVDPGGMSTAKGGFAGFVQRERTKPIIAAVD
                     GPALAGGTEIVLACDLVVASETAVFGVPEVKRNLVAAGGGLFRLPRKIPRNIAMELIL
                     TGRLDFSAAEAHRYGLVNHLSPEGSALARATELAEQICEAAPLAVVESRQIVLETTDA
                     DDEIGWKRSGEGIIKMFGTEDFSEGLNAFIEKRPPEWKNK"
     gene            3799..4002
                     /locus_tag="VNQ33_01605"
     CDS             3799..4002
                     /locus_tag="VNQ33_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96820.1"
                     /translation="MPAPGRRLVACPYCHGIDVARMYLGSVHLDSCQCAECGARWDED
                     PRTGEFRGRGARASVVVPRDGAT"
     gene            complement(4066..>4314)
                     /locus_tag="VNQ33_01610"
     CDS             complement(4066..>4314)
                     /locus_tag="VNQ33_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96821.1"
                     /translation="PAEVQEAAAQEPDVAPAEVQEAAAQEAETAEAATPLAEEATASE
                     AVPAPPAETADVVAVPQEVATAATEPGGDAEVPVDPQD"
BASE COUNT          620 a         1515 c         1580 g          599 t
ORIGIN      
        1 tgatcccctc cttcctcaag cgggtcgatc tggctgatcg aggcgtggcc tggagcgact
       61 acctgggccg caaccgggag gcgatggacg acatcgccga gcgcttcttc ccgtcggccg
      121 acgatgtgca ggaggcgccg cgcacgacgc tcgtggactt cgaccccgat gccgagatcc
      181 acatggtcac ggcgatgctc tacgcccacg cgcacgcccc ggagcgccag atcgaggagc
      241 gggtgcgcca gatgtccgcc gaggagcggc tgtcggtcgt gcgggcctac gtcggcgagc
      301 gggcgaaccg ccgccaccgg ccgggccggg cgctcgagcg catcgactac cggttcgacg
      361 tcctcgccga ctacggcgcc ttccgcgacc tccaacgcca ccggatgctc acgatcgagt
      421 ggcagccgct caccccgctc cacggctaca cccggcccga ggccgtggac ctggccggcg
      481 agaccgaggc gttcgaccgg gcgatggagc gttcggcgtc gctctacgag ctgttggcga
      541 cccgcttccc ggccgaggcg tcctatgcgg tgtgcctggc ctacaaggtg cgcttcacga
      601 tgcagatgaa cgcacgggag gccatgcacc tcctcgagct gcgcagcggc ccccagggcc
      661 atccggcgta ccgcgaggtg gcgcaggaca tgcaccgcct catcgccacc gaggccgggc
      721 accacgccgt ggccgagatg atgcggtacg tcgatcactc gccggagccc agcctggagc
      781 ggctcgactc cgagcgaagg gccgaggccc ggcgcgaagg tcgatgatcc gtccaggatg
      841 cggcatcgcc gcgtctcgcg cgtagtatcc gcccgcggca tcgctcgacg gtgctgccga
      901 ccactcaccc agaaccgggc agacgaagga agacatggct gacgacgagc tgatcgacga
      961 cgagatcgac gtcgaggaag acgacgaact gatcgacgag atcgacgacg agctcgaagg
     1021 tgatgacctc gaggatgacg acgacgtcgt ccccgacgag gagctggtcg cgtcggtggt
     1081 cgacgacgac gaggatgccg aggaagagcc cccgcctgcg ccggtcaagg gccgctccgg
     1141 cgacgacgac gaagacgacg atgacgacgt cgaccccgac gatgtcgagg aggacctcga
     1201 caccatcttg aaggaccgca tcgcggccgg tgccgacgac gacgatgacg acgacgagga
     1261 agcgccggtc gacgaaggcc ccggcggcga ccgggtcgcg ccgcgcgccg ccgaggagtt
     1321 ctcgtgcaac gagtgcttct tgctcgtccg ccgcagccag ttcagcgcca agcggaccga
     1381 ctgcccggga ggcctcgacg gcgaggactg cccggtgaag cgggagctgg ggctggtctg
     1441 agcacccgcc ccgtcgatcg cggcgacgag gcgagatttc gaccccgctc ggcccttgca
     1501 ccaacatggg ccggtgagcg acgacaagaa ccccatcgag cacgcggtcg agctgacggt
     1561 cctggcaccg atcgggctcg ccctcgaggc gaagaggctg ctgccgacgt tcgtggagcg
     1621 cggtcgtcag caggtcaaca tggccaaggt catcggccag ttcgcggtgg cccacggcca
     1681 gacgaccgcc acccagaagc tcacccaggc ccaggcgcag gccgcctcga tcctcgccga
     1741 gttcggcctg gtcggctcgg gggaggacgg tgcggtccca gacgacgcgc cagccgcccc
     1801 ggcggagccg accgaaccgg cggcgcccgc tgccccccgg tcgagcgagg cggcggccga
     1861 cctgcccatc gcggactacg acagcctcgc cgcgtcgcag gtgatcccac ggctcgcggg
     1921 gctctcggcc gcagagctgg ccgcagtcga ggcctacgag gtcggccacc gcggccgcaa
     1981 gacgatcctc ggcaagatca cgcagctcgg cgccgagtag gccggtggag gccgcgcggg
     2041 ctgccctcct gagcgacgtc gcggccatca ccgaactggc gcgcaccgcg gtcggggagc
     2101 tcggcctgct gcggggcggg ccggtctggc agctccaaga tgcccgggtc gagcccatcg
     2161 aagacggcgt ggccgccgac gtggcgctcc agcccgacga ggggctcgcg gtcgtcggga
     2221 ccatcgacgg ggccatcgtc ggctacgggg tcagccggct cgagacgctg gccgacgggt
     2281 cggtcctcgc cgaggtctcc gacctctacg tcgaccccgg ggcccggggc atcgggctgg
     2341 gcgaggcgat gatggacctc ctggtggccc atgccgagca ggccggcgcc atcggcatcg
     2401 acgccctcgc ccttccgggc gaccgggcga ccaagaactt cttcgagacg ttcgggctca
     2461 aggcgcgtgc catcgtggtg caccggtcgt tgcaggcgac cgagcttccc cagggccccg
     2521 gaggagacga tggaagcgcc tgagctctgc gtcagcgccg tggtgatcga cgacgaccgc
     2581 ctgctcctgg tccgccgcgg gcgcggccgg gcccagggcg agtgggccgt tcccggcggc
     2641 cgggtccggc cgggcgagct cctggccgag gccgtggtac gggaactgat cgaggagacc
     2701 ggcatcgaag gcgtctgcgg caacctcatc ggcgtgctcg agctgctcga cgatgccggt
     2761 ccccaccagg tcgtgctggg tcacctcgtg acgctgatgg aggccaccga cccggtggcc
     2821 ggagacgacg ccgccgaggc ccgctgggtg ccgctgagcg acgtcgccga gctccggctc
     2881 gcatccggtc tggccgagtt cctccacgac cacgaggtga tcgccaccat cacgtgatgg
     2941 cggggggcag gactacttgt tcttccactc cgggggccgc ttctcgatga aggcgttgag
     3001 cccttcgctg aagtcttcgg tgccgaacat cttgatgatg ccctcgcccg agcgcttcca
     3061 gccgatctcg tcgtcggcgt cggtggtctc caggacgatc tggcggctct cgacgacggc
     3121 caggggagca gcctcgcaga tctgctcggc cagctcggtg gcccgggcca gggcgctgcc
     3181 ttcggggctg aggtggttga ccaggccgta gcggtgcgcc tcggcggccg agaagtccag
     3241 ccggccggtg aggatcagct ccatggcgat gttgcggggg atcttgcggg ggaggcggaa
     3301 gaggccgccg ccggcggcga cgaggttgcg cttcacctcg ggcacgccga acaccgcggt
     3361 ctccgacgcg accaccaggt cgcacgccaa gacgatctcg gtaccgccgg cgagcgcggg
     3421 gccgtcgacg gcggcgatga tcggcttggt ccgctcgcgc tgcacgaagc cggcgaagcc
     3481 gcccttggcg gtggacatgc cgccgggatc gacggccatc tgcttcaggt cggcgccggc
     3541 gcagaagatc caccccttgg ccgtctgggc gccggtgagg atgccgaccc aggcgttcgg
     3601 gtcctcctcg agctggtcga tcgccgcttc gatgccctgg gcgacgtcgg tgttgacggc
     3661 gttgcgggcg tcgggacggt tgatggtgat gacggcgatg tggccgcgca gttcgtagtc
     3721 gacggtcatg gtggcgacgc tacccgcaac ttgaccggtc agtcaggttg gtggtgccgg
     3781 gcgggtacgg tggcctcgat gcccgcacct ggtcgccgtc tcgtcgcctg tccctactgc
     3841 cacgggatcg acgtggcccg gatgtacctc ggctccgtcc acctcgactc gtgccagtgc
     3901 gccgagtgcg gtgcgcgctg ggacgaggat ccccgcacgg gggagttccg cggccgcggc
     3961 gctcgcgcct cggtcgtggt gcctcgcgac ggcgccacct gaccccggac gcggaggatg
     4021 ggccgccgcc gcgggccccg tcctcgtgca ccgctggcga ggggttcagt cctgggggtc
     4081 gaccgggacc tcggcgtcgc cacccggctc ggtcgccgcg gtggcgacct cctgcggcac
     4141 cgcgacgacg tcggcggtct cggccggcgg ggccggaacc gcctcagaag cggtggcctc
     4201 ctcggcgagc ggggtggcgg cctcggcggt ctcggcctcc tgcgctgcgg cctcctgcac
     4261 ctcggcggga gccacatcgg gctcctgcgc tgcggcctcc tgcacctcgg cggg
//
LOCUS       DATMFS010000086         4949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_282217, whole genome shotgun sequence.
ACCESSION   DATMFS010000086 DATMFS010000000
VERSION     DATMFS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282217"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VNQ33_01615"
     CDS             complement(<1..396)
                     /locus_tag="VNQ33_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96822.1"
                     /translation="MLGRIASFTVRRRRLVLGLALLAFVVSAAIGGGVAKRLSSGGFE
                     DPGAESTAANRILEDEFKTGTPNIVLVVTANDGDVDDPAVAAAAKDLTAELAAETSGG
                     KAMAEVLSYWDLPAGNPLASTDGSKALVLG"
     gene            complement(575..943)
                     /locus_tag="VNQ33_01620"
     CDS             complement(575..943)
                     /locus_tag="VNQ33_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96823.1"
                     /translation="MTANPIRRRAVRVVTAVALSLVVLSGCTTAQHAPKSYAGVEDDF
                     LEGCVKVAESDNQEAQAKTTEIASPQSYCQCVFDAIEKKIPYSDFKKAQSTMQDEGGK
                     IPAKFIDAYESCDPAAKADS"
     gene            complement(1326..1694)
                     /locus_tag="VNQ33_01625"
     CDS             complement(1326..1694)
                     /locus_tag="VNQ33_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96824.1"
                     /translation="MDAPAGRPEPPEDHHGDDPGIAADYDAEVLAALDAWEPAESGPP
                     TLPTRLVSWRRRSVIGAVAMGWALGIREVLETDPDDPIVIELDADGEPHDLPIRLLLD
                     PDDPSGSLCFVRRDPPPPVI"
     gene            complement(1704..2408)
                     /locus_tag="VNQ33_01630"
     CDS             complement(1704..2408)
                     /locus_tag="VNQ33_01630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ96825.1"
                     /translation="MVVADPGADLLRLVTAAGDPPPAEPTPAGARSAALIRRRFGLAL
                     PPPFVGHPGPDEPVRIATATDGPAIAAVKWRAFGANYRGGVLDDDFLDHRGVVPPASF
                     WVGRAMLPPSRRHRLLVWGRPGTVFGYLDAGPVHPEDVDRDAPESGEVYELYVDPVVQ
                     GRGGGGRLLDAAEAWFRSAGFERAELSTLVTNPAAQGFYRSRGWEPTGRIIPVDLGVV
                     AFEEMRFARRLDGARS"
     gene            complement(2409..2852)
                     /locus_tag="VNQ33_01635"
     CDS             complement(2409..2852)
                     /locus_tag="VNQ33_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HWJ96826.1"
                     /translation="MLISTTFDLAGYDVTATLGEVFGLTVRSRNIGAGCIAGFRSIAG
                     GEIPEFTKLLVQSRNEAMARMVTEAQARGANAVIGMRFDGGAFGQWSEICAYGTAVWV
                     EPQTDYAKRQHEAVQAHGQMPHQSTYGVKVSEWEAGSGAGRPPGT"
     gene            complement(3036..3911)
                     /gene="rsmI"
                     /locus_tag="VNQ33_01640"
     CDS             complement(3036..3911)
                     /gene="rsmI"
                     /locus_tag="VNQ33_01640"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWJ96827.1"
                     /translation="MSSSSASTSDPAGPSAASSGRLVLVGTPIGNLGDLTPRAVETLA
                     SADLVCCEDTRRTGRLLQHAGIRAAALRVVNDHTEDGAVDDVLARLGRGETVAVVSDA
                     GMPGISDPGERLVAAAAHAGFTIEVVPGPSAAVTALVASGLPAGRFAFEGFLPRKGSG
                     RPQRLAAIATERRTVVLYEAPHRVERTLADLLAACGPERRVVLARELTKLHEEHWRGT
                     LAEAAAHTAEVEPRGEYVLVLDGAPAPAEVTDDDIVAAIDRAKAAGASTRDAVAEAAA
                     ELNVPKRRAYDLANR"
     gene            complement(3883..>4949)
                     /gene="ychF"
                     /locus_tag="VNQ33_01645"
     CDS             complement(3883..>4949)
                     /gene="ychF"
                     /locus_tag="VNQ33_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWJ96828.1"
                     /translation="GFVGLPNSGKSSLYNALAGGGALAAPYVFATTDPNVGVAKVPDR
                     RLDLLAEMSQSKKVVPASVQFVDIGGLVEGASTGEGLGNQFLAHIREVDTVVFLLRAF
                     TDTDVPGPDDPLECLRVLEIELALADLETVESRVEKRRKAAKQDKSLTELVEALDAAH
                     ELLADGTPIYRGDLTDDQREVLKQEFLLTNKPVLALVNVGEDDLERIPEIIAPVEAEL
                     GDRAEVIGMCVQLEAEAAQFTGAERLEMLEALGLGDGGLERFIHAAYQLMGLRTFLTT
                     GEKESRAWTFRAGYRAPQCAGRIHTDFERGFIRAEVIHWDELLELGSWNKAKDVGKLR
                     VEGKDYVVEDGDVLEFRFNV"
BASE COUNT          708 a         1775 c         1776 g          690 t
ORIGIN      
        1 gcccagcacc agcgccttgc tcccgtccgt cgacgccagc gggttgcccg ccggcaggtc
       61 ccagtacgag aggacctcgg ccatcgcctt gccgcccgac gtctccgccg ccagctccgc
      121 ggtcaggtcc ttcgccgcgg cggcgacggc cggatcgtcg acgtcgccgt cgttggccgt
      181 gacgaccagg acgatgttcg gcgtgcccgt cttgaactcg tcctcgagga tccggttggc
      241 ggccgtggac tcggcacccg ggtcctcgaa cccgccgctc gagaggcgct tggcgacgcc
      301 accgccgatg gcggccgaca ccacgaacgc cagcagggcg aggccgagga cgagccgacg
      361 gcggcggacg gtgaacgatg cgatccgacc cagcatgagg agactcccgc gttgtgtaaa
      421 cgtcgtttac atatcgctcc tcagccaacc agcgggcccc ggatgtgtcc agatgacgtc
      481 agtcacccgc cgtgaccagc gccgcacggg cgcgccgaag caggcggctc gcgggtctgc
      541 gggctcgggg cctgcgggac ccggcggcct caggtcagga gtcggccttc gccgcgggat
      601 cgcacgactc gtacgcatcg atgaacttgg ccgggatctt cccgccctcg tcttgcatcg
      661 tggactgcgc cttcttgaag tccgagtagg ggatcttctt ctcgatcgcg tcgaagacgc
      721 actggcagta gctctgcggc gaggcgatct cggtcgtctt cgcctgcgcc tcctggttgt
      781 cgctctcggc gaccttcacg cagccctcga ggaagtcgtc ttcgactccg gcgtagctct
      841 tgggagcatg ctgcgccgtg gtgcagccgc tgaggacgac gagcgacaag gcgacggcgg
      901 tcaccacgcg gacggctcga cgacggatcg ggtttgcggt cacgcctggc tccttgttca
      961 cgacccagcc acgctagtgc cggaccggcc gtcgaccgaa gtcgggccgg gaccaaccgc
     1021 cgtcggaagg cacctccgcg gcgtcgcggc gaacgtggcc ccggacgcgg gacgagggtg
     1081 ccggcgagcc ggcaccctcg acaccattcc gaccgacgct ccccccgaac cgcctgccgg
     1141 acttctcccc ccgcaccttg cggtgcagca cgatccttga tcaaggaaca agattcgctg
     1201 ggaaaggatt caacacccgc agccgcccgc gcacatgggc cgaacgacct attttcgagt
     1261 aaatgttcgg attttgtgaa catcggtcac cccgcaggtg gagcggaggt gacgcccgtg
     1321 tcacatcaga tcaccggcgg cggtggatcg cggcgcacga agcacaggct ccccgacgga
     1381 tcgtcgggat cgagcaggag gcggatgggg agatcgtgcg gctcgccgtc agcgtcgagc
     1441 tcgatgacga tcggatcgtc ggggtcggtc tcgaggacct cgcggatgcc gagcgcccag
     1501 cccatcgcca ccgcgccgat gaccgagcgg cggcgccagg acaccagccg ggtcgggagc
     1561 gtcggcggcc cggactcggc gggctcccag gcgtcgagcg ccgccagcac ctcggcgtcg
     1621 tagtccgcgg cgatcccggg atcgtcgccg tggtgatcct ctggcggctc gggccgaccc
     1681 gctggcgcgt ccatggcctc aggctacgag cgggctccgt cgaggcgacg agcgaagcgc
     1741 atctcctcga aggcgaccac gcccaggtcc accgggatga tgcggcccgt cggctcccac
     1801 ccccgggagc ggtagaagcc ctgcgccgcc gggttggtga cgagcgtcga cagctccgct
     1861 cgttcgaagc cggcggaccg gaaccacgcc tcggccgcat cgagcagccg gccgccgccg
     1921 cctcggccct gcaccaccgg atcgacgtac agctcgtaca cctcgccgct ctccggcgcg
     1981 tcgcgatcga catcttcggg gtggaccggc ccggcatcga ggtagccgaa caccgtcccc
     2041 ggacgacccc acacgaggag ccggtgccgg cgcgacggcg gcagcatcgc ccggcccacc
     2101 cagaaggatg ccggcggcac gacgccgcgg tggtcgagga agtcgtcgtc caacacgccg
     2161 ccgcggtagt tggctccgaa cgcccgccac ttgaccgcgg cgatggcggg cccgtcggtg
     2221 gcggtggcga tgcgcacggg ctcgtcaggg ccagggtgcc cgacgaaagg cggcgggagc
     2281 gccaggccga accgacggcg gatcagcgcc gcggaccggg caccggccgg cgtgggctcg
     2341 gcgggcggcg gatcgccggc agcggtgacg aggcgcagca ggtccgcgcc cggatcggcg
     2401 acgaccactc aggtccccgg cggccggccc gcaccggacc cggcctccca ctcgctgacc
     2461 ttcacgccgt aggtcgactg gtgcggcatc tggccgtggg cctgcacggc ctcgtgctgg
     2521 cgcttggcgt agtcggtctg cggctcgacc cagacggccg tgccgtaggc gcagatctcc
     2581 gaccactggc cgaaggcgcc gccgtcgaag cgcatgccga tcacggcatt ggcgccccgg
     2641 gcctgcgcct cggtgaccat gcgcgccatg gcctcgttgc ggctctggac gaggagcttg
     2701 gtgaactcgg ggatctcgcc gccggcgatg gagcggaacc cggcgatgca gccggcgccg
     2761 atgttgcggg agcggacggt gaggccgaac acctccccga gggtggcggt cacgtcgtac
     2821 ccggcgaggt cgaaggtggt ggagatgagc atggcccgga ccgtagaccg gcacccccag
     2881 gcgcgcgtcc gacgctcacc ctccacgatt tcgggtcacc tgggcacctc accggtgcac
     2941 gaacgacccg aaaccacccg ccgggcccga cccacgcgat ttcgggtcac ctgggcaccc
     3001 cataggtgca tgatcgaccc gaaatcgggt gggtgtcagc ggttggcgag gtcgtaggcg
     3061 cggcgcttgg ggacgttgag ctcggcggcg gcctcggcga cggcgtcgcg ggtcgatgcg
     3121 ccggccgcct tggcccggtc gatggcggcg acgatgtcgt cgtcggtgac ctcagccggg
     3181 gcgggggcgc catcgaggac cagcacgtac tcgccgcgcg gctcgacctc ggcagtgtgg
     3241 gcggcagcct cggcgagcgt gccccgccag tgctcctcgt ggagcttggt cagctcgcgt
     3301 gcgagcacca cgcggcgttc aggaccacag gcggcgagca ggtcggccag cgtccgctcg
     3361 acccggtggg gcgcctcgta gagcacgacc gtccgccgct cggtggcgat ggcggcgagg
     3421 cgctggggcc gccccgaccc cttgcggggc aagaacccct cgaacgcgaa gcgacccgcc
     3481 ggcaggccgc tggcgacgag cgccgtcacc gcggcggacg ggccggggac gacctcgatc
     3541 gtgaagccgg cgtgcgcggc cgctgccacc agccgctcgc ccgggtcgga gatgccgggc
     3601 atcccggcgt cggacacgac cgccacggtc tcgccgcggc cgagccgggc gagcacgtcg
     3661 tcgacggcgc cgtcttcggt gtggtcgttc acgacgcgca gggcggcagc acggatgccg
     3721 gcgtgctgca gcagccggcc cgtgcggcgg gtgtcctcgc agcagacgag gtcggccgac
     3781 gccagcgtct cgacggcgcg cggggtcaag tcgccgaggt tcccgatggg cgtcccgacg
     3841 agcaccagcc ggcccgagct cgcggccgat ggcccggccg ggtcagacgt tgaagcggaa
     3901 ctcgaggaca tcgccgtcct ccaccacgta gtccttgcct tccacgcgca gcttgcccac
     3961 gtccttggcc ttgttccacg agccgagctc gagcagctcg tcccagtgga tgacctcggc
     4021 ccggatgaac ccgcgctcga agtcggtgtg gatccgcccg gcgcactgcg gcgccctgta
     4081 cccggcgcgg aacgtccagg cccgggactc cttctcgccg gtggtgagga aggtgcgcaa
     4141 gcccatcaac tggtaggcgg cgtggatgaa ccgctcgagc ccgccgtcgc ccaggccgag
     4201 cgcttcgagc atctccagcc gctcggcgcc ggtgaactgg gcggcttcgg cctccagctg
     4261 cacgcacatg ccgatgacct cggcccggtc gccgagctcg gcctccaccg gggcgatgat
     4321 ctcggggatc cgctccaggt cgtcctcgcc cacgttcacc agcgccagca ccggcttgtt
     4381 ggtgagcagg aactcctgct tgagcacctc gcgctggtcg tcggtgaggt cgcctcggta
     4441 gatcggcgtg ccgtcggcca gcagctcgtg ggcggcgtcg agcgcctcga ccagctcggt
     4501 cagcgacttg tcctgcttgg cggccttgcg gcgcttctcg acccggctct cgacggtctc
     4561 caggtcggcc agggccagct cgatctccag cacccggagg cactcgagcg ggtcgtcggg
     4621 accaggcacg tcggtgtcgg tgaacgcccg caggaggaac acgacggtgt cgacctcgcg
     4681 gatgtgggcc aggaactggt tgcccaggcc ctcgcccgtg ctggccccct cgaccaggcc
     4741 gccgatgtcg acgaactgca cgctggcggg cacgaccttc ttcgactggg acatctcggc
     4801 cagcaggtcc aggcggcggt ccggcacctt ggccacgccc acgttggggt cggtggtggc
     4861 gaagacgtag ggcgcggcga gcgccccgcc accggcgagc gcgttgtaga gcgaggactt
     4921 gcccgagttg gggaggccga cgaagccga
//
LOCUS       DATMFS010000087         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_26677, whole genome shotgun sequence.
ACCESSION   DATMFS010000087 DATMFS010000000
VERSION     DATMFS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26677"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VNQ33_01650"
     CDS             complement(<1..488)
                     /locus_tag="VNQ33_01650"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ96829.1"
                     /translation="MKHPKIVTLGAVAVGLAAAAAGTYRATHPKDASGPLRPDPGRAR
                     RPVDDVLFDLPAGVIEHEIPTPDGGTVHAIEQGAGRPLVLLHGITLRADVWAPQFHQL
                     TDRYRVIAVDLRGHGTSKAGDAGFGVARLGDDLATLLTELDLHDAVLVGHSMGGMTVM
                     QF"
     gene            complement(485..1615)
                     /gene="alr"
                     /locus_tag="VNQ33_01655"
     CDS             complement(485..1615)
                     /gene="alr"
                     /locus_tag="VNQ33_01655"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWJ96830.1"
                     /translation="MRPVWAEVDLGAISANVATLRERSAPAAMCAVVKADGYGHGAVP
                     VARAALAGGADVLAVALVAEGVELRDAGIDAVVMVLSQASADEIDTLVAADLDATVYT
                     ASGVAGLADAARRAGRTADRPVRVHLKVDTGMHRVGVQPEDAVALAKTVADEPALELC
                     SVFTHCAVADEPGNPFTAKQLKQFDRVLADLDAGGIDVACTHAANTAAAIDHPASRRD
                     LVRCGIGIYGLAPAPALAGRVELVPAMSLHARVSHVKRVAAGEGVSYGLRYRPRTETT
                     IATVPLGYADGLPRRLGSNGGTVLVGGVPRPIAGSVTMDQILVDCGDAPIEVGDPVVL
                     LGRQGGASIGAEDWAALLGTIAYEIVCGISPRVPRRYVEATS"
     gene            complement(1628..2086)
                     /locus_tag="VNQ33_01660"
     CDS             complement(1628..2086)
                     /locus_tag="VNQ33_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ96831.1"
                     /translation="MTTDVLQFAPGDNVGDVMAKLVERGIDGAPVVDSDNQVVGIIST
                     GDLIVQETELHVPTIISLFGATLELPSAHKHFEDDLRKTLGATVADVMQPNPVTASPD
                     DTIEAAATKLHEHNVSRLPVVGDGGLVGIISRVDVLREIIRDDEAPAPDA"
     gene            complement(2091..>2750)
                     /locus_tag="VNQ33_01665"
     CDS             complement(2091..>2750)
                     /locus_tag="VNQ33_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HWJ96832.1"
                     /translation="PVEAVVAPLAGPSWSAEVLDGLERFHALGVGPGLGTDEDTADQV
                     RQLVAGAALATVVDGDGLRALGAAAATVIRRRPADAPLVVLTPHDGEFAALDGGAPGA
                     DRFAAVRDLARATGTVVLLKGPATLVGAPDGSVLVTDQGDARLATAGSGDVLTGLIVG
                     LLASGVPPPRAAAAGAFLHGRAGDLAWRRGLVAADLVGHLPAAFAEVAGGSIATLYPR
                     L"
BASE COUNT          372 a          942 c         1098 g          338 t
ORIGIN      
        1 cagaactgca tcacggtcat gccgcccatc gagtggccga cgagcacggc atcgtgcagg
       61 tcgagctcgg tcaggagcgt cgccaggtcg tcgccgagcc gggcgacgcc gaacccggcg
      121 tcgcccgcct tcgaggtgcc gtggccgcgg aggtcgaccg cgatgacgcg gtaccggtcg
      181 gtgagctggt ggaactgcgg agcccacacg tcggcacgga gcgtgatgcc gtggaggagc
      241 acgaggggac ggcccgcgcc ctgctcgatg gcgtggaccg tgccgccgtc gggcgtgggg
      301 atctcgtgct cgatgacacc ggcgggcagg tcgaacagca cgtcgtcgac cgggcggcgg
      361 gcacgaccgg ggtcggggcg caacggaccg gacgcatctt tgggatgcgt cgcccggtac
      421 gtgcccgcag cggccgcggc cagtccgacg gcgacggcgc cgagggtgac gatcttgggg
      481 tgcttcacga ggtggcctcc acgtagcgac gggggacgcg cgggctgatc ccgcagacga
      541 tctcgtaggc gatggtgccg agcagggccg cccagtcctc agcgccgatc gatgcaccgc
      601 cctgtcgccc gaggagcacg acggggtcac cgacctcgat gggtgcgtcg ccgcagtcga
      661 cgaggatctg gtccatcgtg accgagccgg cgatcggccg gggcaccccg ccgacgagca
      721 ccgtgccgcc gttgctgccc aggcgacggg gcaggccgtc ggcgtagccg agcgggacgg
      781 tggcgatcgt ggtctcggtg cgcgggcggt agcggaggcc gtaggagacg ccctcccccg
      841 ccgcgacccg cttcacgtgc gagacgcgcg cgtgcagcga catcgccggg accagctcga
      901 cccgcccggc cagcgccggg gccggggcca ggccgtagat gccgatgccg cagcggacca
      961 ggtcgcggcg ggatgcgggg tggtcgatgg ccgccgcggt gttggcggcg tgggtgcacg
     1021 cgacgtcgat cccccccgca tcgaggtcgg ccagcacgcg gtcgaactgc ttcagctgct
     1081 tggcggtgaa cgggttcccg ggctcgtcgg cgaccgcgca gtgggtgaac accgagcaca
     1141 gctcgagggc cggctcgtcg gcgacggtct tggcgagggc caccgcgtcc tcgggctgga
     1201 cgccgacgcg gtgcatcccg gtgtcgacct tgaggtggac gcggaccggt cgatcggcgg
     1261 tccgcccggc gcgccgggcg gcgtcggcca ggccggccac acccgacgcc gtgtagacgg
     1321 tggcgtcgag gtcggccgcg accagggtgt cgatctcgtc ggcggaggcc tgcgagagca
     1381 ccatgacgac ggcgtcgatc ccggcgtcgc gcagctcgac gccctccgcg accaaggcga
     1441 cggcgaggac gtcggctcct ccggcgagcg cggcccgggc gaccgggacg gcaccgtggc
     1501 cgtagccgtc ggccttcacg accgcacaca tcgcggcggg cgccgatcgc tcgcgcagcg
     1561 tggccacgtt ggctgagatc gcgccgaggt cgacctcggc ccagacgggg cgcaccgggg
     1621 gtgggtccta ggcgtcgggc gccggggcct cgtcgtcgcg gatgatctcg cgcagcacgt
     1681 cgacccggga gatgatgccg acgagacctc cgtcgcccac cacggggagg cgcgacacgt
     1741 tgtgctcgtg gagcttcgtg gccgcggcct cgatggtgtc atcaggcgag gcggtcaccg
     1801 ggttgggctg catgacgtcg gcgacggtgg cgccgagcgt cttgcggagg tcgtcctcga
     1861 agtgcttgtg cgcgctgggc agctcgaggg tggcgccgaa gagcgagatg atcgtgggca
     1921 cgtggagctc ggtctcctgc acgatcaggt caccggtgga gatgatgccc accacctggt
     1981 tgtccgagtc cacgaccggc gcgccatcga tgccgcgctc gaccagcttg gccatgacgt
     2041 caccgacgtt gtcccccggc gcgaactgga ggacatcggt ggtcatgact tcagagacgg
     2101 gggtagagcg tggcaatcga gcctccggcg acttcggcga acgcggccgg gaggtggccg
     2161 acgaggtccg cagccaccaa gccgcggcgc caggctaggt ccccggcccg cccgtgcagg
     2221 aaggcaccgg ctgccgcggc gcgcggcggc ggcacgccgg aggcgagcag gccgacgatg
     2281 agaccggtca agacgtcgcc gctgccggcc gtggcgaggc gggcgtcgcc ctgatcggtg
     2341 acgaggacgg acccgtcggg ggcgccgacg agggtggccg ggcccttgag cagcacgacg
     2401 gtcccggtcg cccgcgccag gtcccggacc gcggcgaacc ggtcggcccc gggggcaccg
     2461 ccgtcgaggg cggcgaactc gccgtcgtgg ggggtgagca ccaccagcgg ggcatcggca
     2521 ggccgtcgcc ggatcacggt cgctgccgcg gcgccgaggg cgcggaggcc gtcgccgtcg
     2581 acgaccgtcg cgagcgccgc cccggcgacc agctgccgaa cctggtcggc tgtgtcctcg
     2641 tcggtgccga ggccggggcc gacgccgagg gcgtggaagc gctccaagcc gtcgagcacc
     2701 tcggcggacc acgacggccc ggcgagcggg gcgacgacgg cttcgacggg
//
LOCUS       DATMFS010000088         4135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_305545, whole genome shotgun sequence.
ACCESSION   DATMFS010000088 DATMFS010000000
VERSION     DATMFS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305545"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VNQ33_01670"
     CDS             <1..772
                     /locus_tag="VNQ33_01670"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HWJ96833.1"
                     /translation="LDLTPEQRRKLSREEVASMFDNGIRFEAVLEAGFALRIAQADDI
                     TDPRITYLCHEMGEETRHQRLFQRVIQEIRPTARNPLAANILIRWFDRFGTRWILNHP
                     ATLYTMVISGEEIPDLLQKLASEHPDTDPFLASVNRYHRQEEARHLSFARSMLAEVWA
                     DAGRGERWGIHHVVPVAIRQMYEFLVHPGVYETVGLEGWPTWKAVNKLPERIAVREQA
                     TRPILGALIDAGMISPKHVPKGWQNLCGVPGRVDRSTL"
     gene            complement(858..3062)
                     /locus_tag="VNQ33_01675"
     CDS             complement(858..3062)
                     /locus_tag="VNQ33_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96834.1"
                     /translation="MQALVADEAKVTLVDAADDVVLEHSFEEGPPIVMVDLPVDTNFD
                     ELRALVTLPDEAEPTEFNVDRVVQPGSAVPRIVISRQTASDPEVTELLEHDPEKLGGE
                     PTELSLPVNPFRPRQVFRYFQISPGLRASEWGSGEAKDYEMSWRSLGWALDDWVSTRS
                     LDPMQTMATAHASISQDSTITQSGGTSNREGVGSERQSFDGSDQRLDATTEMSGSTRS
                     MGVGANLSVNVAPLAAGPAGALGKTIGGAVQAGVGVASSEGKTTSSATIGKRIVSAVQ
                     DAAQRQRVVESESGRQAHHGLADDRILTASSNPNPSTENVCRYSIVHQWRVKTRRVRV
                     VPIKLVPIQGLTEKFDLETVVANRAILLEHLLDERYAALLSTVEIADGQRAPDPPSPH
                     PIKSISFDIEFSDPPRAKDGTFKLQIHHSGGPPLEASLKGKVERGEHWTGHLLPPNGA
                     TLTVSTLQKLEFVFSNPGLITVDTAVTIARGLITWRATGEDADRTLAHLGQTRVPHDE
                     HRWITITPVVPGMVEPGLPRDVSALLAHLNANLAHYRTEIDLARHPVERFVSLGDKAE
                     ELADMEPIGVVGAHLAFEAPATGTSPGDSEEPVVDVVSIATGATWEEAISGKSKIRER
                     ALTARWTNLPAADTKAVAWPPVGGLTMPGELASPQDDTTLTAPAHEAADAGDPPTMAE
                     VTAMADKLNAALKDLTAAAQAAAAAPAAGTGAAAPPADGEGDDDGDDGDDGK"
     gene            3243..>4135
                     /locus_tag="VNQ33_01680"
     CDS             3243..>4135
                     /locus_tag="VNQ33_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ96835.1"
                     /translation="MRVEQVALPVLRQINRRPRIAPVLDTLLKPWNPFSPTLNVDPYP
                     SYEVLRSKGPVLWHRALNTWMVSSFELCETAFRAQVSVDRGDLFDVVPPWSKLTRSDR
                     ETFTRSVLLLDPPDHTRLRRIVSRAFTPRTVERIEPRVNEIAWELVDKVRHERTVDLF
                     ESVFAPLPIYVIGELLGIPERDWPRLKVWSDEFAKVIDPVDAFDPREMGVAIDEMKTA
                     LDGWIDLRAAEPGDDLLSQLIAAEDEGGRLSRTELQSMIALLMTAGHETTSGLLGNAV
                     VALADRPDLRDRLAHDPAVAAP"
BASE COUNT          575 a         1456 c         1434 g          670 t
ORIGIN      
        1 gctggacctc acgcccgagc agcgtcggaa gctgagccgg gaggaggtgg cctcgatgtt
       61 cgacaacggc atccggttcg aggccgtcct cgaggccggg ttcgccttgc gcatcgccca
      121 ggccgacgac atcaccgacc cgcgcatcac ctacctctgc cacgagatgg gagaggagac
      181 ccgccaccag cggctgttcc agcgggtcat ccaggagatc cgccccaccg cccgcaaccc
      241 gctggccgcc aacatcttga tccgctggtt cgaccggttc gggacccgct ggatcctcaa
      301 ccacccggcc acgctctaca cgatggtcat ctccggcgag gagatccccg acctgctcca
      361 gaagctggcg agcgagcacc ccgacaccga cccgttcctc gcctcggtca accggtacca
      421 ccgccaggag gaggcccggc acctgtcgtt cgcccggtcg atgctggccg aggtgtgggc
      481 cgatgccgga cggggcgagc gctggggcat ccaccacgtg gtgcccgtgg ccatccgcca
      541 gatgtacgag ttcctggtgc accccggcgt ctacgagacg gtcggcctcg aaggctggcc
      601 cacctggaag gccgtcaaca agctccccga gcgcatcgcc gtccgggagc aggcgacccg
      661 cccgatcctc ggggctctga tcgacgccgg catgatctcg cccaagcacg tcccgaaggg
      721 atggcagaac ctctgcggcg tccccggccg agtggaccgc tccaccctct gacctcaccg
      781 cgcggcgtcg cacgtcgcac caaaggcctg gatccttccg gactcggggg caggtcggtg
      841 catgagcccg actcggccta cttgccgtcg tccccgtcgt ccccgtcgtc gtctccctcg
      901 ccgtcggcgg gaggcgcagc ggccccggtc ccggcggctg gggccgccgc cgcggcctgg
      961 gcggcggccg tcaggtcctt cagcgcagcg ttgagcttgt cggccatcgc cgtcacctcg
     1021 gccatcgtcg gtggatcgcc ggcgtcggcg gcctcgtggg ccggcgccgt gagggtggtg
     1081 tcatcctgcg gggaggccag ctcgcccggc atggtgagac cgccgaccgg cggccaggcg
     1141 acggccttcg tgtcggcagc cggcaggttg gtccaccgag cggtcagggc gcgctctcgg
     1201 atcttggact tcccggagat ggcctcctcc cacgtcgcgc cggtggcgat cgagaccacg
     1261 tcgacgacgg gctcctcgga gtcacccggg cttgtccccg ttgccggcgc ctcgaacgcg
     1321 aggtgagcgc ccacgacgcc gatcggttcc atgtcggcga gctcctcggc cttgtcgccg
     1381 agcgagacga agcgctcgac cgggtggcga gccaggtcga tctcggtccg gtagtgggcg
     1441 aggttcgcgt tgaggtgggc gagcagagcg gagacatctc ggggcagtcc gggctccacc
     1501 atccccggga cgacaggcgt gatggtgatc cagcggtgct cgtcgtgggg gactcgtgtc
     1561 tggccgaggt gggccagcgt ccgatccgcg tcttcaccgg ttgctcgcca ggtgatcagg
     1621 ccccgggcga tcgtcaccgc ggtgtcgacg gtgatgaggc ccgggttgga gaacacgaac
     1681 tccagcttct gcagcgtcga caccgtgagg gtcgcgccgt tcggcgggag gaggtggccg
     1741 gtccagtgtt cgcctcgctc caccttgccc ttcaagctgg cctcgagcgg cggcccgccg
     1801 ctgtggtgga tctgcagctt gaaggtgccg tctttcgccc gaggcgggtc ggagaactcg
     1861 atgtcgaacg agatcgactt gatgggatgg ggcgagggcg ggtcgggggc gcgctggccg
     1921 tcagcgatct cgacggtgga gagcagggcc gcgtagcgct cgtccagcag gtgctcgagc
     1981 aggatcgcgc ggttggccac cacggtctcc aggtcgaact tctcggtgag gccctggatc
     2041 ggtacgagct tgatcgggac gacccgcacc cggcgcgtct tgaccctcca ctggtggacg
     2101 atgctgtacc ggcacacgtt ctcggtgctg gggttcggat tgctcgatgc cgtgaggatc
     2161 cggtcgtcgg ccagtccgtg gtgggcctgc cggccgctct cgctctcgac gacgcgctga
     2221 cgctgggccg cgtcttgcac cgcggagacg atccgcttgc cgatggtcgc cgagctggtg
     2281 gtcttgccct ccgacgacgc caccccgacc ccggcttgga ccgcaccgcc gatggtcttc
     2341 ccgagcgctc ccgccggacc tgcggcgaga ggggcgacgt tgacggacag gttggcgccg
     2401 acccccatcg accgggtgct gccgctcatc tcggtcgtcg cgtcgaggcg ctggtccgat
     2461 ccgtcgaacg actgccgctc gctgccgacg ccttcgcggt tggaggtgcc tccgctctgg
     2521 gtgatcgtcg agtcctggct gatcgacgcg tgggcggtcg ccatggtctg catcggatcg
     2581 agcgaccggg tggagaccca gtcgtcgagt gcccacccga gcgagcgcca ggacatctcg
     2641 tagtccttcg cctcgccgct gccccattcc gacgcccgga ggccgggact gatctggaag
     2701 taccggaaca cctggcgagg ccggaacggg ttgaccggga gggagagctc ggtcggttcc
     2761 ccacccagct tctccgggtc gtgctcgagc agttcggtca cctcaggatc cgatgccgtc
     2821 tgccgggaga tcacgatgcg cggcacggcg ctgcccggct gcaccacgcg gtccacgttg
     2881 aactccgtcg gctcggcctc gtcggggagc gtcacgaggg cgcgcaactc gtcgaagttg
     2941 gtgtcgacgg gcaggtcgac catgacgatc ggcggcccct cctcgaacga atgctcgagg
     3001 acgacgtcgt ccgcggcgtc gaccaacgtg accttcgcct catccgccac gagcgcctgc
     3061 aacagaatct gatgcggatt gctcccgacc atggtcccac ctccatgtcg caccgttgtg
     3121 cgagcagcct tgcacgattg cccgatgcgt gcaccgaaaa cgaccggacg ccgtgtgcgc
     3181 cgacccgtcg acgtgaccga tgcccggtga ccgactcggg tgagcgtggg atcatcttgg
     3241 tcatgcgtgt cgagcaggtg gcgttgccgg tgcttcggca gatcaatcga cggccccgga
     3301 tcgcgccggt gctggacacg ctgctgaagc cgtggaaccc gttcagcccg acgctcaacg
     3361 tcgacccgta cccgtcgtac gaggtgctgc ggtcgaaggg gccggtcctg tggcaccggg
     3421 cgctcaacac ctggatggtg tcgtcgttcg agctgtgcga gacggccttc cgggcgcagg
     3481 tgtcggtcga tcggggggac ttgttcgacg tcgtgccccc gtggtcgaag ctgacccggt
     3541 ccgaccggga gaccttcacc cggtcggtgc tcctcctcga cccgcccgat cacacccgcc
     3601 tccgccgcat cgtgtcccgg gcgttcacgc cccgcaccgt cgagcgcatc gagccccggg
     3661 tcaacgagat cgcctgggag ctggtggaca aggtgcgcca cgagcgcacg gtggacctgt
     3721 tcgagtcggt cttcgccccg ctgccgatct acgtgatcgg cgagctgctc ggcatccccg
     3781 agcgggactg gccccggctg aaggtgtggt cggacgagtt cgccaaggtg atcgaccccg
     3841 tcgacgcgtt cgacccgcgg gagatgggcg tcgccatcga cgagatgaag acggcgctgg
     3901 acggctggat cgacctccgt gccgctgagc ccggcgacga cctcctctcc cagctcatcg
     3961 ccgccgagga cgagggcggc cgcctcagcc ggaccgagct ccagtcgatg atcgccctcc
     4021 tcatgaccgc gggccacgag accaccagcg gcctgctcgg caacgccgtc gtcgccctgg
     4081 ccgaccggcc cgacctgcgt gaccgcctgg cgcacgaccc ggccgtcgcg gcgcc
//
LOCUS       DATMFS010000089         6049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_26760, whole genome shotgun sequence.
ACCESSION   DATMFS010000089 DATMFS010000000
VERSION     DATMFS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26760"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..555
                     /locus_tag="VNQ33_01685"
     CDS             232..555
                     /locus_tag="VNQ33_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96836.1"
                     /translation="MVIPTAPVVAGSLIGGYYVARTTKNRKLGAAVLGVGGVYAKTEW
                     MKTSGPVTTAILSGVFYGGFGLSHPLAKKIGAWPSVLTVAGIAGAAAYALNDRKAGAI
                     ESVPA"
     gene            complement(688..2316)
                     /locus_tag="VNQ33_01690"
     CDS             complement(688..2316)
                     /locus_tag="VNQ33_01690"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWJ96837.1"
                     /translation="MNQGEPERERWVLPEVGRRRFLIGAAAATTVVLVGCGSGDRDGS
                     SGPPDGSTTAPRKAVDPVDAPAGVDGSPFTLGVASGDPTSESVILWTRLALDPTADDG
                     SGGMADDDVAIAWDLAADEDFTEVLASELIAAKAAHGHSVHVDAKGLDPATTYWYRFR
                     VGDQTSPVGQTATLPTGSPEAFGLAVVNCQMYEGGAWGAYRNLLDEDVDLVVHLGDYI
                     YEYPGGEGDGRHSLPNRMLTTLADFRLRYASYKLDPDLQAAHARFPFVATWDDHEVAN
                     NYMGDTVPGDATPEQVEERKAAAYQAWWENMPARLDPPDGPDLAVYRDFEVGDLAKLY
                     LLDERQDSAEPPCRDESTSGLDYGNCDEVESEDRTRLGKDQEAWLSDSLTKSTARWNL
                     LGNPVVLAGVDAGKDEPQFYLDTWDGFPGARKRLIAQLAEVDNPVVLTGDYHAGMVLD
                     VHAVPFDQDTPVVAPEFMSPPISSALFDADVTKRTPQIRQQINEYGYLVVAVTPDELT
                     ATFRVVDDVADPKTGISSAATWKVAAGNPVAEKS"
     gene            2416..3339
                     /locus_tag="VNQ33_01695"
     CDS             2416..3339
                     /locus_tag="VNQ33_01695"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HWJ96838.1"
                     /translation="MDQTALLNELEPVAEGLLNKHLASSKEWFPHELVPWSRGRDFEP
                     GEAWTADEAPMDPAVRSSLFVNLLTEDNLPYYFRTIEALFGADSAWGTWVRRWTAEEG
                     RHSIVLRDYLTVTRMLDPVALERARMHQVEGGEVPVVDFVTDGIVYVTLQELATRIAH
                     HNTGKLLTDKAGYEVMKRVAADENRHHLFYRDLGTAALAIDPSAMVIAMERQVREFEM
                     PGTGIPDFESHAHAIARAGIYDFTVHHDSVLVPVILRHWDVEHLEGLSPEAEVARKAL
                     VKRMERIARVGRRFTAKRDEAVATEAELVTA"
     gene            complement(3447..5630)
                     /locus_tag="VNQ33_01700"
     CDS             complement(3447..5630)
                     /locus_tag="VNQ33_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWJ96839.1"
                     /translation="MSDGDAADLNELEPTRRTGSLKTRSLVLGGVLVVAAGAALFTWR
                     QVKPVVESRKYASVTYEVPKAPKLTPAEGETVYRIDPTESSLTYQVKERFAGKETSSA
                     KGTTNGIAGDIAIDPSDLSATRVGKVVVNLEQLHSDNNLRDARLRLDYLESHEHPLAS
                     FEPKEITGLDGKLADGKAQDFSMTGDLTVKEITKPATFTGTATLEDGALVVKASSKAK
                     LSDFGAGPINIAGMVSTSDDATLTLKLTAYDPTERTIPTRITGPDAKEPTGGPSFAKT
                     VQPILEANCVSCHNTGQMGAHTLTIDTAGDARSVSDGLKTVTQLKHMPPWPASDVGVP
                     LKHKMSLTQAEIDAIAKWSDAGAPLDVSPEAFIKAKPTEPGPTPREDIVLDRPAYTGS
                     MDNTNDYRCFVLDPKLTEPTYLTGYTFLADQIEELHHAQVFHISAEQVASSKEVDGKD
                     GQPGWGCYSGPNLRGQRPSKVPGRAPKRDVGFSGQANLVAGWVPGQGPSDFGKDTGIL
                     MEPGDALVLQLHYHYSSEPTPDRSTLALQVEPGTKDLKALRVINPIGPVEIPCAPEDQ
                     DQPLCDREAALADNVELYGPSGAANEAGLLMLCGKTPEQLTKDFDGKVASSSCDQTVP
                     EDGTIVAVLGHMHTIGKTFRLTLDPDTKDEKVLLDIPEWSFDWQMNYELETPIHVKAG
                     QPLRMDCSWDRSADPLRKPKYIVFAEGTEDEMCFATYALIPDDQG"
     gene            complement(5849..>6049)
                     /locus_tag="VNQ33_01705"
     CDS             complement(5849..>6049)
                     /locus_tag="VNQ33_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96840.1"
                     /translation="PPVTLTVAGRRPVRLTAWAGPWPVDERWWDPARHRRRARFQVVD
                     QEGAAHLLAVEAGRWWREATYD"
BASE COUNT          826 a         2061 c         2181 g          981 t
ORIGIN      
        1 tacatcgccc gccagctcta ccgccagctc gaacaacccc ttgacgacct ataggagcgt
       61 cgccaccccg tccaccgagc gtcaccgctc agcgcgaccg atcgggtcgt cgtggcccac
      121 ggagctcgag cagggccggc acctcggagc gaccccgcgg ctcagcgcgc ccatcgggga
      181 gcgctcgcct gcgaggttcc cccgggtcct ggggttggta ggttcggcgc catggtcatc
      241 cccaccgctc ctgtcgtcgc cggctcgctc atcgggggct actacgtcgc ccgcaccacc
      301 aagaaccgca agctcggcgc ggcggtcctc ggcgtcggcg gcgtctacgc caagaccgag
      361 tggatgaaga cgagcgggcc ggtcaccacc gcgatcctct cgggcgtgtt ctacggcggc
      421 ttcggcctgt cgcacccgct ggccaagaag atcggcgcct ggccgtcggt gctcaccgtc
      481 gccggcatcg ccggtgccgc cgcctacgcc ctcaacgacc gcaaggccgg cgcgatcgag
      541 tccgtccccg cctgatccgg gctttcgggt cacctgggca cccctacggt gcacgcgtga
      601 cccgaaatcg accgagcccc ggccacccga cgctttcggg tcacctgggc acccctgcgg
      661 tgcacgcgtg acccgaaatc gggagggtca ggacttctcg gcgacggggt tgccggcggc
      721 gaccttccag gtggctgccg agctgatgcc ggtcttcggg tccgcgacgt cgtcgacgac
      781 gcggaacgtg gcggtcagct cgtcgggcgt gacggccacc accaggtagc cgtactcgtt
      841 gatctgctgg cggatctgcg gcgtgcgctt ggtgacgtcg gcgtcgaaca gggccgacga
      901 gatcggcggc gacatgaact cgggcgccac caccggcgtg tcctggtcga acggcacggc
      961 gtggacgtcg agcaccatgc cggcgtggta gtcaccggtg agcacgaccg ggttgtcgac
     1021 ctcggccagc tgggcgatga ggcgcttgcg ggcaccgggg aagccgtccc aggtgtcgag
     1081 gtagaactgc ggctcgtcct tgccggcgtc gacgccggcg agcacgaccg ggttcccgag
     1141 gaggttccac ctcgccgtgc tcttggtgag cgagtccgac agccacgcct cctggtcctt
     1201 gccgaggcgg gtgcggtcct cgctctcgac ctcgtcgcag ttcccgtagt cgaggccgga
     1261 ggtggattcg tcccggcagg gcggctcagc gctgtcttgg cgctcgtcga gcaggtacag
     1321 cttggccagg tcgcccacct cgaagtcccg gtacaccgcg aggtcggggc cgtccggcgg
     1381 gtcgagacgg gccggcatgt tctcccacca cgcctggtag gcggcggcct tgcgctcctc
     1441 cacctgctcg ggcgtggcat cgcccgggac ggtgtcgccc atgtagttgt tggcgacctc
     1501 gtggtcgtcc caggtggcga cgaacgggaa gcgggcgtgg gccgcttgga ggtcggggtc
     1561 gagcttgtag gacgcgtagc ggaggcggaa gtcggccagc gtcgtgagca tccggttcgg
     1621 cagcgagtgc cggccgtcgc cctcaccgcc cgggtactcg tagatgtagt cgccgaggtg
     1681 gacgacgagg tcgacgtctt catcgaggag gttccggtag gcgccccacg cgccgccctc
     1741 gtacatctgg cagttcacga ccgcgagccc gaacgcctcc ggcgagcccg tcggcaaggt
     1801 ggcggtctgc ccgaccgggc tggtctggtc gccgacgcgg aaccggtacc agtacgtcgt
     1861 ggcgggatcg aggcccttgg cgtcgacgtg gacggagtgg ccgtgggcgg ccttggccgc
     1921 gatcagctcc gaggcgagca cctcggtgaa gtcctcgtcg gcggcgaggt cccaggcgat
     1981 ggcgacgtcg tcgtcggcca tcccgcccga tccgtcgtcg gcggtcgggt cgagcgcgag
     2041 gcgggtccag aggatgaccg actccgatgt cgggtcgccc gaggccacac cgagcgtgaa
     2101 cggcgagccg tcgacgccgg ctggcgcatc gacggggtcg accgccttgc gaggcgccgt
     2161 ggtcgagccg tcgggtggcc cgctcgaccc gtcgcggtca ccggacccgc accccacgag
     2221 cacgaccgtg gtcgccgcgg ccgcaccgat caggaagcgg cgccgcccga cctcgggcag
     2281 cacccagcgt tcacgttccg gttccccctg gttcatgtca ggagtctggc acgtgcgaag
     2341 gcgtccgagc ggcccggacg cacgtcacag cgcccgggct cgcctcccta cctaccggta
     2401 ggtaggctgc tgttcatgga ccagaccgcc ctcctcaacg agctcgaacc ggtcgccgag
     2461 ggcctgctga acaagcacct cgcctcctcc aaggagtggt tcccccacga gctggtgccg
     2521 tggagccgcg gtcgggactt cgagcccggc gaggcctgga ccgccgacga ggccccgatg
     2581 gacccggccg tgcggtcgag cctcttcgtc aacctcctca ccgaggacaa cctcccctac
     2641 tacttccgca ccatcgaggc cctgttcggc gccgacagcg cgtggggcac ctgggtccgt
     2701 cggtggacgg ccgaggaggg tcgccactcc atcgtgctgc gcgactacct gaccgtcacc
     2761 cggatgctcg atccggtcgc gctcgagcgg gcccgcatgc accaggtcga gggtggtgag
     2821 gtaccggtgg tcgacttcgt caccgacggc atcgtctacg tgacgctcca ggagctggcc
     2881 acgcgcatcg cccaccacaa cacgggcaag ctcctcaccg acaaggccgg ctacgaggtg
     2941 atgaagcggg tggccgccga cgagaaccgc caccacctct tctaccgcga cctcggcacc
     3001 gcggcgctgg cgatcgaccc gtcggccatg gtcatcgcca tggagcgcca ggtgcgcgag
     3061 ttcgagatgc ccggcaccgg catccccgac ttcgagtccc acgcccacgc catcgcccgc
     3121 gccggcatct acgacttcac cgtccaccac gactccgtgc tggtcccggt gatcctgcgc
     3181 cactgggacg tcgagcacct cgagggcctc agccccgagg ccgaggtcgc ccgcaaggcc
     3241 ctggtgaagc gcatggagcg catcgcccgc gtcggccgcc gcttcaccgc gaagcgcgac
     3301 gaggccgtcg ccaccgaagc cgagctcgtc accgcctgat cgaaggcaac cgagaccgaa
     3361 ctttcgtagg tgagcacgac ccttcggttg ccctgaccta cgaagttcgc atcagcgggg
     3421 ttctgcggac cggcggccgg cagcgatcag ccctggtcgt cggggatgag ggcgtacgtg
     3481 gcgaagcaca tctcgtcttc ggtcccctcg gcgaacacga tgtacttcgg cttgcgcagc
     3541 ggatcggcgc tgcggtccca cgagcagtcc atccgcagcg gctggcccgc cttcacgtgg
     3601 atgggcgtct ccagctcgta gttcatctgc cagtcgaagc tccactccgg gatgtcgagc
     3661 agcaccttct cgtccttcgt gtccgggtcg agcgtcaggc ggaaggtctt tccgatcgtg
     3721 tgcatgtggc cgagcacggc gacgatggtg ccgtcttcgg gaaccgtctg gtcgcacgac
     3781 gacgacgcca ccttgccgtc gaagtccttg gtcaactgct cgggcgtctt gccgcagagc
     3841 atgagcaggc cggcctcgtt ggccgcaccc gacgggccgt agagctccac gttgtcggcg
     3901 agcgccgcct cgcggtcgca cagcggctgg tcctggtcct cgggggcaca ggggatctcg
     3961 accggcccga tcgggttgat cacccgcagg gccttgaggt ccttggtgcc gggctcgacc
     4021 tggagggcga gcgtcgagcg gtccggcgtg ggctcgctgc tgtagtggta gtggagctgg
     4081 agcaccaggg catcgcctgg ctccatgagg atgccggtgt ccttgccgaa gtccgacgga
     4141 ccctggcccg gcacccagcc ggcgacgagg ttggcctggc ccgagaagcc gacgtcgcgc
     4201 ttcggagcgc ggccggggac cttggacggg cgctggccgc ggaggttggg gccgctgtag
     4261 cagccccaac ccggctggcc gtccttgccg tcgacctcct tcgacgaggc cacctgctcg
     4321 gcgctgatgt ggaacacctg ggcgtggtgg agctcttcga tctggtcggc gaggaacgtg
     4381 tagccggtga ggtaggtggg ctcggtgagc ttcgggtcca gcacgaagca gcggtagtcg
     4441 ttcgtgttgt ccatcgagcc cgtgtaggcg gggcggtcca agacgatgtc ctcgcgcggg
     4501 gtgggaccgg gctcggtggg cttggccttg atgaacgctt caggcgagac gtcgagcggg
     4561 gcgccggcat cggaccactt ggcgatggcg tcgatctcgg cctgcgtcag cgacatcttg
     4621 tgcttgagcg gcacgcccac gtcggaggcc ggccacggcg gcatgtgctt gagctgggtg
     4681 acggtcttga ggccgtcgga caccgagcgg gcgtcgccgg cggtgtcgat ggtgagggtg
     4741 tgggcgccca tctggccggt gttgtggcac gagacgcagt tggcctcgag gatcggctgc
     4801 acggtcttgg cgaacgacgg gccacccgtg ggctccttgg cgtcgggccc ggtgatcctg
     4861 gtggggatgg tgcgctcggt ggggtcgtag gcggtgagct tcagcgtgag ggtggcgtcg
     4921 tcggaggtcg agaccatgcc ggcgatgttg atgggaccag cgccgaagtc cgagagcttg
     4981 gccttgctcg aggccttcac cacgagggcg ccgtcctcga gggtcgcggt gcccgtgaac
     5041 gttgccggct tggtgatctc cttgacggtg agatcaccgg tcatggagaa gtcctgggcc
     5101 ttgccgtcgg ccagcttccc gtcgaggccg gtgatctcct tcggctcgaa gctggcgagc
     5161 gggtgctcgt gggactcgag gtagtcgagg cggagccggg cgtcgcgcag gttgttgtcg
     5221 gagtggagct gctcgaggtt gacgacgacc ttgccgacgc gggtggccga caggtccgag
     5281 gggtcgatgg cgatgtcgcc ggcgatgccg ttggtggtgc ccttcgccga gctggtctcc
     5341 ttgcccgcga atcgctcctt cacctggtag gtgagcgacg actcggtggg gtcgatgcgg
     5401 tagacggtct cgccctcggc cggggtgagc ttcggcgcct tcggcacctc gtaggtgacc
     5461 gaggcgtact tgcgcgactc gacgacgggc ttcacctggc gccaggtgaa cagcgcggcg
     5521 ccagccgcga cgacgaggac gccgcccagc acgagcgagc gggtcttgag cgagccggtg
     5581 cggcgcgtgg gctccagctc gttgaggtct gcggcgtcgc cgtcgctcat gtcggttccc
     5641 ttcggtgctg ccccccggca gcacgcagct cggccatctt aggtgcgcgg gcgcgctcta
     5701 tttgcatcat cggtgcgaaa tgtgaggagg ttgaggtcac gaccgcgggg acggaccacc
     5761 agggctcctg cgaacgcttc gcgatcgcgc ttgccctgcc aggtcgcccc gccaccagcg
     5821 tcaccgagag cccgctcgcg acgggcagtc agtcgtaggt cgcttcgcgc caccagcggc
     5881 cggcttccac ggccagcagg tgggcggcgc cctcctggtc gacgacctgg aagcgggccc
     5941 gccggcggtg gcgggcgggg tcccaccagc gctcgtcgac tggccacggc ccggcccacg
     6001 ccgtgagccg caccgggcgc cggccggcca ccgtgagggt gaccggcgg
//
LOCUS       DATMFS010000090         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_120242, whole genome shotgun sequence.
ACCESSION   DATMFS010000090 DATMFS010000000
VERSION     DATMFS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120242"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..749)
                     /gene="cysC"
                     /locus_tag="VNQ33_01710"
     CDS             complement(177..749)
                     /gene="cysC"
                     /locus_tag="VNQ33_01710"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWJ96841.1"
                     /translation="MSTNVTWHDGEVSREDRPTPGATVWLTGLSGSGKSTVGVALERL
                     LVAAGRPAYRLDGDNLRLGLNADLGFTAEDRDENVRRAGEVAKLLADAGVVAIVPLIS
                     PYRAGRDRARTVHASAGVPFVEVFVDTPIELCEQRDPKGLYAKARAGEISGFTGVDDP
                     YEAPEAPELVLTPADGDPDAQARAILALLR"
     gene            complement(760..2028)
                     /gene="cysN"
                     /locus_tag="VNQ33_01715"
     CDS             complement(760..2028)
                     /gene="cysN"
                     /locus_tag="VNQ33_01715"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HWJ96842.1"
                     /translation="MSELLRFATAGSVDDGKSTLIGRLLYDSKAIFEDQMEAVERSSE
                     AMGSEHTNLALLTDGLRAEREQGITIDVAYRYFATPRRKFVIADTPGHIQYTRNMVTG
                     ASTADLALVLVDARKGLVEQSRRHAFLASLLRIPHLVLCVNKMDLVDWDEQRFEEIKA
                     EFRSFAAKLDITDLAFIPVSALHGDNVVTRSENTPWYEGSSLLHHLEEVHIASDRNLI
                     DVRFPVQYVIRPHNDEFHDYRGYAGTVAGGVLKPGDPVTVLPSGFSSTIASVETMDGP
                     RAEAYAPESVTVRLTDEIDISRGDMICRPNNQPTSGQDIDAMVCWMSEASSLKDRDKL
                     AIKHTTRTARALVKNVQYRLDVNTLHRDDEAASLALNEIGRVQLRTTQPLFYDEYRRN
                     RTTGSFILIDEATNTTVGAGMILGPSAPQP"
     gene            complement(2153..>2439)
                     /locus_tag="VNQ33_01720"
     CDS             complement(2153..>2439)
                     /locus_tag="VNQ33_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HWJ96843.1"
                     /translation="YYAHEREVFQRDGMWLAVSEHLAPGEDEEVVVKTVRYRTVGDAT
                     ITGAVESDAATVEAVVAEVAATRLTERGATRADDRVTEAAMEDRKKEGYF"
BASE COUNT          334 a          854 c          847 g          404 t
ORIGIN      
        1 atccccatcc gcggcggcag gcgaggctcc ggcagcggcc caggccaccg ccgcgaccgc
       61 cacgacgacg cgcgcggcct tcactcggac cgatcccatc gagccccctc acctcgccgg
      121 acccgaacga cggtagcgcg cccgaacggc tcgcccgatc cctctcgacg tggccgctac
      181 cggaggaggg cgaggatggc tcgggcttgg gcatcggggt cgccgtcagc cggggtgagg
      241 accagctcgg gcgcctcggg cgcctcgtag gggtcgtcga cgccggtgaa gccggagatc
      301 tccccggcgc gagccttggc gtagaggccc ttcggatcac gctgctcgca cagctcgatg
      361 ggcgtgtcga cgaagacctc gacgaacggc acccccgccg acgcgtgcac cgtccgggcc
      421 cggtcgcggc cggcccggta cgggctgatg agcgggacga tggccaccac gccggcgtcg
      481 gcgagcagct tggcgacctc gccggcgcgg cggacgttct cgtcgcggtc ttccgcggtg
      541 aagccgagat cggcgttgag gccgagccgg aggttgtcgc cgtcgagacg gtacgccggc
      601 cggccggccg ccacgagcag tcgctcgagc gcgacgccga cggtcgactt gcccgagccc
      661 gacagacccg tgagccacac ggtcgcaccc ggcgtcgggc ggtcctcccg gctcacctcg
      721 ccgtcgtgcc aggtgacgtt ggtgctcacg accggctgct cacggctggg gtgcggacgg
      781 accgaggatc atgccggcgc ccaccgtggt gttggtggcc tcgtcgatga ggatgaacga
      841 gccggtggtc cggttgcggc ggtactcgtc gtagaacagc ggctgggtgg tccgcagctg
      901 cacgcggccg atctcgttga gcgccagcga tgctgcctcg tcgtcgcggt gcagcgtgtt
      961 cacgtcgagg cggtactgca cgttcttcac gagcgctcgg gccgtgcggg tcgtgtgctt
     1021 gatggcgagc ttgtcccggt ccttcagcga cgacgcctcg ctcatccagc agaccatggc
     1081 gtcgatgtct tggcccgagg tcggctggtt gttcgggcgg cagatcatgt cgccgcggct
     1141 gatgtcgatc tcgtcggtga ggcggacggt cacggactcg ggcgcgtagg cctcggcccg
     1201 cggaccgtcc atcgtctcca cgctcgcgat cgtcgacgag aagcccgacg ggagcaccgt
     1261 cacgggatcg ccgggcttga gcacaccgcc ggccaccgtc ccggcgtagc cgcggtagtc
     1321 gtggaactcg tcgttgtggg gtcggatcac gtactggacc gggaaccgca cgtcgatgag
     1381 gttccggtcc gacgcgatgt gcacctcctc caggtggtgc agcagcgacg acccctcgta
     1441 ccagggcgtg ttctcggacc gggtcacgac gttgtcgccg tgcagggccg ataccgggat
     1501 gaacgcgagg tcggtgatgt cgagcttggc ggcgaagctc cggaactcgg ccttgatctc
     1561 ctcgaaccgc tgctcgtccc agtcgacgag gtccatcttg ttcacgcaca gcaccaggtg
     1621 cgggatccgc agcagcgacg ccaagaaggc atggcggcgg gactgctcga cgagtccctt
     1681 gcgggcgtcg accagcacga gcgccaggtc ggcggtggac gcacccgtga ccatgttgcg
     1741 cgtgtactgg atgtgccccg gggtgtcggc gatgacgaac ttccggcggg gcgtggcgaa
     1801 gtagcggtag gccacgtcga tcgtgatccc ctgctcgcgc tcggcccgca ggccgtcggt
     1861 cagcagcgcc aggttcgtgt gctcggagcc catcgcctcc gacgaccgct ccaccgcctc
     1921 catctggtct tcgaagatgg ccttcgagtc gtacagcagc cgcccgatca gcgtcgactt
     1981 cccgtcgtcc accgacccgg ccgtggcaaa cctcaagagc tcggacatca gaaggagccc
     2041 ccgtcgtcga tgttcgctcg aagctccttc gtcgccccgc tcacctcgtc ttcaagcgcg
     2101 cccactcgcc gctcaccttc ctgctgcgaa cccggctgca tcctggtgac catcagaagt
     2161 agccctcctt ctttcggtcc tccatggctg cttcggtcac ccggtcgtcc gcccgggttg
     2221 cgcctcgctc ggtgaggcgt gtggcggcga cttcggcgac cacggcctcg acggtggccg
     2281 catcgctctc gaccgcgccg gtgatggtgg cgtcgccgac cgtccggtac ctcacggtct
     2341 tgacgacgac ctcttcgtcc tcgccgggtg cgaggtgctc gctgacggcg agccacatgc
     2401 cgtcgcgctg gaacacctcg cgctcgtggg cgtagtaga
//
LOCUS       DATMFS010000091         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_73183, whole genome shotgun sequence.
ACCESSION   DATMFS010000091 DATMFS010000000
VERSION     DATMFS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73183"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VNQ33_01725"
     CDS             complement(<1..947)
                     /locus_tag="VNQ33_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HWJ96844.1"
                     /translation="MRCEVVAIGTELLLGQIVDTNSSWIGEQLAIAGIDSLFQTKVGD
                     NVDRMVATLELALSRSDAVICCGGLGPTQDDITREAIAEVMGVDLELDDEVAARIEAM
                     FTSRGRRMPMSNLRQAEVPVGATAIRDPQPGTAPGLICPVTWDGQEKVLYAVPGVPRE
                     MEELVVDAVIPDLQRRSGDTSTIFSRTLRTWGESESGLAERLAGRIAVLDGTGNPTLA
                     YNASGIEGIKLRLTAKGPDLASAEAVVAEEEREIRAILGDLVFGVDGETIEASVLKEL
                     RLAGERLGLGRPLTLGLAESLTGGMMGSRVADVPGASDV"
     gene            complement(1105..1722)
                     /gene="pgsA"
                     /locus_tag="VNQ33_01730"
     CDS             complement(1105..1722)
                     /gene="pgsA"
                     /locus_tag="VNQ33_01730"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWJ96845.1"
                     /translation="MALGDELGTPGPVPIDDRYGPGALLTPANMITMLRLVLTPALLV
                     MIVEKPTSWAAAAFWTVLAFSDGIDGHLARKHGTTRSGAFLDPLADKVLVLGALFALV
                     AAGRFWIVPVLLIAAREIAISMYRTHLARQGLAVPARPLGKLKTVVQEFAVAFALFPI
                     TGDYHLLATVVLWVAVGLTYVSGAQYLLDGRSAATTMARRPATAS"
     gene            complement(1730..>2270)
                     /locus_tag="VNQ33_01735"
     CDS             complement(1730..>2270)
                     /locus_tag="VNQ33_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWJ96846.1"
                     /translation="TGVPYFDLSLQHVSPPLVRRMRRWGNGEKFLRRIDDIRAREPEA
                     AFRSNFIVGYPGETEDDHDQLLRFVAEAEVDWCGFFAYSPEDGTYAADLDDQVDPELV
                     ADRLAELRELQDGITAAKRDDLIGSRVEVLVDTPGTARSHREAPEIDGVIGVPGHLAA
                     GTLATLTVTGAMGPDLEAE"
BASE COUNT          330 a          791 c          822 g          327 t
ORIGIN      
        1 aacacatcgg acgcgcccgg cacgtcggcg acgcgcgagc ccatcatccc gccggtgagg
       61 gactcggcga gcccgagggt gaggggccgc ccgagcccca ggcgctcccc cgccaggcgc
      121 agctccttca gcacggatgc ctcgatcgtc tcgccgtcca cgccgaacac gaggtcgccg
      181 aggatggccc ggatctcccg ctcctcctcg gccaccaccg cctcggccga ggcgagatcg
      241 ggacccttcg ccgtgagccg gagcttgatg ccctcgatgc cgctggcgtt gtaggcgagc
      301 gtcgggttgc ccgtgccgtc gagcacggcg atgcgcccgg ccagccgttc ggccaggccc
      361 gactcgctct cgccccacgt ccgcagggtg cggctgaaga tggtggacgt gtcgcccgac
      421 cgccgctgca ggtccgggat gacggcgtcg accaccagct cctccatctc ccgcgggaca
      481 ccgggcacgg catagagcac cttctcctgg ccgtcccagg tgaccgggca gatgaggcca
      541 ggagcggtgc cgggctgcgg gtcgcggatg gccgtcgccc ccacgggcac ctcggcctga
      601 cggaggttgc tcatcggcat ccgccggcct cgtgacgtga acatcgcctc gatgcgcgcc
      661 gccacctcgt cgtcgagctc caggtccacc cccatcacct cggcgatggc ctccctggtg
      721 atgtcgtcct gggtggggcc gaggccgccg cagcagatga cggcatccga gcggctgagg
      781 gccagctcga gcgtggcgac catccggtcc acgttgtcgc cgaccttggt ctggaacagc
      841 gagtcgatcc cggcgatggc cagctgctcg ccgatccacg aggagttggt gtcgacgatc
      901 tgcccgagca gcagctccgt cccgatggcg accacttcgc agcgcatgcg ccgcacccta
      961 cgtcgcccga ccttcgttca tttcgggtca cgcgtgcacc catgaggtgc ccaggcgacc
     1021 cgaaatggtg tggccgccgg cttccacctt gtttcgggtc acgcgtgcac ccttggggtg
     1081 cccagacgac ccgaaatcgc tgggtcagga ggcggtggcg gggcggcggg ccatggtcgt
     1141 ggcggccgag cggccgtcga ggaggtactg ggcgccggac acgtaggtga ggccgacggc
     1201 gacccagagg acgaccgtgg cgagcaggtg gtagtcgccg gtgatcggga agagggcgaa
     1261 ggcgacggcg aactcctgga ccaccgtctt cagcttgccc agcggccggg cggggacggc
     1321 gaggccctgc cgggccaggt gcgtccggta catgctgatg gcgatctcgc gggccgcgat
     1381 caacaagacg gggacgatcc agaaccggcc ggcggccacg agggcgaaca gcgcgccgag
     1441 gacgaggacc ttgtcggcca gcgggtcgag gaaggcaccg gagcgggtgg tgccgtgctt
     1501 gcgggccagg tggccgtcga tgccgtcgct gaacgccagc accgtccaga acgcggcggc
     1561 cgcccaggag gtgggcttct ccacgatcat cacgagcagc gccggcgtca gcacgagccg
     1621 gagcatcgtg atcatgttgg ccggcgtgag cagcgcgccg gggccgtacc gatcgtcgat
     1681 gggcaccggt cccggggtgc ccagctcgtc ccccaacgcc atcgcttcgc tactccgctt
     1741 ccagatcggg gcccatcgcc ccggtgacgg tgagcgtggc caacgtacct gccgcgaggt
     1801 ggccggggac accgatgacc ccgtcgatct ccggggcttc tcggtgcgag cgcgccgtgc
     1861 cgggggtgtc cacgagcact tcgacccgcg aaccgatgag atcgtcacgt tttgccgcgg
     1921 tgatgccgtc ttgcagctcg cgcagctcgg cgaggcgatc ggccaccagt tccgggtcga
     1981 cctggtcgtc gaggtcggcg gcgtaggtgc cgtcttcggg cgagtaggcg aagaagccgc
     2041 accagtcgac ctcggcctcg gcgacgaacc ggaggagctg gtcgtggtcg tcctcggtct
     2101 cgccggggta gccgacgatg aagttcgacc ggaacgcggc ctcgggctcc cgggcgcgga
     2161 tgtcgtcgat gcggcgcagg aacttctcgc cgttccccca gcgccgcatg cgccgcacga
     2221 gcggcggcga cacgtgctgc agggagaggt cgaagtacgg gacccccgtg
//
LOCUS       DATMFS010000092         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_329237, whole genome shotgun sequence.
ACCESSION   DATMFS010000092 DATMFS010000000
VERSION     DATMFS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329237"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VNQ33_01740"
     CDS             complement(<1..1024)
                     /locus_tag="VNQ33_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ96847.1"
                     /translation="MDQRTIAVYDREGAGYAARRGVQRPERAAAFASAVDGRDPSAVR
                     IDLGAGPGHYVPLLGTPVVAVDASWTMASAARSAHPGVPVVQADLARLPFGPGTVGGV
                     WASKVHQHLPAADLPLALADVHRALPVGGRFDLTVFAAADGGPTAEEVTDEASGDDLP
                     GRWFTWWAPDHLRRTVEAAGFAVDDLAVVPAQHHDAHPRIEIAATALRALPDHVGAGM
                     RLLCCGLNPSLHAADAGVGYVTGSNRFWKAMAAAGLATRDRDPVHLLRHDRIGMTDLV
                     KRATARADELTTAEYRAGVERLDALCAWLRPQAVAVVGLAGWRAAVDRKASVGWQPQL
                     LGPSPVY"
     gene            complement(1038..1322)
                     /locus_tag="VNQ33_01745"
     CDS             complement(1038..1322)
                     /locus_tag="VNQ33_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96848.1"
                     /translation="MAKRPAKPVVDDGLAAMGLEFGQIVRYAGTSDKRYREGVVLRRE
                     KDGSVGLRDDRGRARAIPVERIEIRMVGPRGGEQWVPLVDTGEGLQLGLF"
     gene            complement(1322..>2047)
                     /locus_tag="VNQ33_01750"
     CDS             complement(1322..>2047)
                     /locus_tag="VNQ33_01750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ96849.1"
                     /translation="EGARHVVVADLAQADVDAAAATLGERASGVAVDVAERAGIAALV
                     AGIEEEHGPIDLFVSNAGIGTGAGVDASDEVWDRIWKVNVLAHVHAAEAVLPSMLARG
                     EGYLLSTASAAGLLSQIGDLPYSVTKHAAVAVAEWLAITYGSQGITVSCLCPQGVRTD
                     LLANAMGISSVETVVSQGLLEPADVARSVVDAVRAEEFLILPHPEVAEYYRRRATDPD
                     RWLAGMARLQARILGTLDEAPGV"
BASE COUNT          263 a          753 c          781 g          250 t
ORIGIN      
        1 cgtagaccgg gctgggtccg agcagctgcg gctgccagcc gaccgaggcc ttccggtcca
       61 cggcggcgcg ccaaccggcc aggcccacga cggcgaccgc ctgcggtcgc agccacgcgc
      121 acagggcgtc gagccgctcg accccggccc ggtactcggc ggtggtcagc tcgtcggcgc
      181 gggccgtggc ccgcttcacc aggtcggtca tgccgatgcg gtcgtggcgg agcaggtgga
      241 ccgggtcgcg gtcgcgggtg gccagcccgg cggccgccat cgccttccag aaccggttgg
      301 agccggtgac gtacccgacg ccggcgtcgg cggcgtgcag cgacgggttg aggccgcagc
      361 acagcaggcg catcccggct ccgacgtggt cggggagggc tcggagcgcg gtggccgcga
      421 tctcgatgcg cgggtgggcg tcgtggtgct gggccgggac cacagccaag tcgtcgacgg
      481 cgaaccccgc ggcctcgacg gtccgccgga ggtggtcggg tgcccaccag gtgaaccacc
      541 ggccgggcag atcgtcgccg ctggcctcgt cggtgacctc ttcggccgtg ggaccgccgt
      601 cggcggcggc gaacacggtg aggtcgaagc ggccgccgac ggggagggct cggtgcacgt
      661 cggcgagggc cagcgggagg tccgcggccg gcaggtgctg gtggaccttc gacgcccaca
      721 ccccgccgac cgtgccggga ccgaacggga gccgtgccag gtcggcctgc accaccggga
      781 cgcccgggtg ggccgagcgc gccgccgatg ccatggtcca cgacgcgtcg acggcgacca
      841 ccggcgtgcc cagcagcggc acgtagtgcc ccgggccggc gcccaggtcg atgcggacgg
      901 cactcgggtc gcggccgtcg acggcggagg cgaaggccgc ggcccgctcg ggccgctgga
      961 cgccccggcg agcggcgtac ccggctcctt ctcggtcgta gaccgcgatg gtgcgctggt
     1021 ccatcagcga ccggccatca gaacaacccg agctgcaggc cttcgccggt gtcgaccagc
     1081 ggcacccact gctcgccgcc gcggggcccg accatgcgga tctcgatgcg ctccaccggg
     1141 atcgcccgcg ctcgcccccg atcgtcgcgg agccccaccg agccgtcctt ctcccgccgg
     1201 agcacgacgc cttcgcggta ccgcttgtcg gacgtgccgg cgtaccgcac gatctggccg
     1261 aactccaggc ccatcgcggc caggccgtcg tcgaccacgg gcttcgccgg gcgcttggcc
     1321 atcagacgcc gggtgcctcg tcgagcgtgc cgaggatgcg ggcctgcagc cgggccatgc
     1381 cggcgagcca gcggtccggg tccgtggcgc ggcgccggta gtactcggcg acctcggggt
     1441 gggggaggat caggaactcc tcggcccgga cggcgtcgac cacggaacgg gcgacgtcgg
     1501 ccggctccag caggccctgc gacacgaccg tctccaccga cgagatcccc atggcgttgg
     1561 cgaggaggtc ggtgcggacg ccctgcgggc acaggcacga cacggtgatg ccctgtgacc
     1621 cgtaggtgat ggccagccac tccgcgacgg cgacggccgc gtgcttggtg accgagtacg
     1681 gcaggtcgcc gatctggctg agcagcccag ccgccgacgc ggtggagagc aggtagccct
     1741 cgccccgggc gagcatcgag ggcagcacgg cttcggccgc gtgcacgtgg gcgaggacgt
     1801 tgaccttcca gatccggtcc cagacctcgt cggatgcgtc gacgccggca ccggtgccga
     1861 tgccggcgtt gctcacgaac aggtcgatgg gcccgtgctc ctcctcgatg ccggcgacga
     1921 gcgcggcgat gccggcccgc tccgcgacgt ccacggccac gccactggct cgttcgccca
     1981 gggttgccgc ggcggcatcg acgtcggcct gggccagatc ggccacgacg acgtggcggg
     2041 cgccctc
//
LOCUS       DATMFS010000093         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_189991, whole genome shotgun sequence.
ACCESSION   DATMFS010000093 DATMFS010000000
VERSION     DATMFS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189991"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VNQ33_01755"
     CDS             complement(<1..1928)
                     /locus_tag="VNQ33_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96850.1"
                     /translation="MTPDEDATPRRAGRVDWLLVAYVAMASAPIVIGCWWAAAHHWIP
                     TGDRAFIALNGDRVLSAHTPTVGMPSTLPVEQADGSAPFVNHLGPMEFWALSVPSLLG
                     GGSTIALLVGVALVNIGSIVLAAWAAVRTLSRAAAGWYMTGCVVLSVGLGLPLLWDNW
                     NPHITIFPLVALLMVASAVVAGHRWFLPWAVLLAVFIAQVHLSTVLIGLVVGAWCLAA
                     GLWQSRRGGGPGVLRRWRGPLLAGTGVIAATWLLPVANEVDSGRGNLTLLIQSMGQGR
                     EQTGPSYAIAMLGRLGSLQPLWLQRATGVFDLPKPTVLDRMWTVVLLGLFVAVLVGAI
                     RTRAADAGRFAVLTTAAVVLVASFADLAMSPSPPVGISYIRWLWAVGLFVWFATGYGV
                     VSFAGEHLRAQRPDLAAAADDRSVVRMVLAMPLVVGLLLVPVAASDPDLPPPDAADDW
                     RALGVLSDQVEASIEGRSVLLAFHGVDAAFSGGPAVWRALDARGVDVRVPANYNGGTS
                     GVWGWEPGEPIDLAAGVYGERTGHLVPREVPIAVLGIGLDDRAQADEWVAAANRLLGE
                     LPPGSRVVLDPATDARLRTEARPDADPDAPVEDQLSDRAKDLDRMLGDARLAMFDREV
                     VRAIAEGRATRSPISPSDA"
     gene            2010..2582
                     /locus_tag="VNQ33_01760"
     CDS             2010..2582
                     /locus_tag="VNQ33_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96851.1"
                     /translation="MVSPRRLAVIVLLAAVAAFAAGCGVDPGDQAASSSSSGPSDRTT
                     TTAPSKDLSPMERAMVDRAKDLYVKLGMDPDDADCLARGLVAAGAGEGQVDPTDTGAM
                     MDIVNQCDIDMSELNDLGSENGITSMEDGLEFGLQSSLESGGLSKKEAECVASDFVDQ
                     YGTDVSAMQDREKVAALMEGCNVDPSKYGG"
     gene            complement(2604..>3313)
                     /locus_tag="VNQ33_01765"
     CDS             complement(2604..>3313)
                     /locus_tag="VNQ33_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWJ96852.1"
                     /translation="LLARPQLFPWVRLDVEGAEHIPDGPAIIVGNHRSYLDPLAIGYA
                     MAEAGRPVRFLGKKEVFDAPVVGQVVTALGGIRVERGSGSDEPLKAAAEALAAGEAVA
                     IMPQGTIPRGRAFFDPELKGRWGAAKLAAMSGAPVIPIGLWGTEKVWPRNARTPDVLA
                     IGHPPTVRIRVGPPVDLGLADPDTDTKAIMAALVDLLPDEARLHREPTPEELARSLPA
                     GYQGDPGHEADRRPGAD"
BASE COUNT          508 a         1223 c         1195 g          387 t
ORIGIN      
        1 gcgtcggacg gcgagatggg cgaccgcgtc gcgcggccct ccgcgatcgc ccggacgacc
       61 tcccggtcga acatcgccag ccgggcgtcg cccagcatcc ggtcgaggtc cttggcccgg
      121 tccgagagct ggtcctccac cggagcatcc gggtcggcgt ccggtctggc ctcggtccgc
      181 agacgggcgt ccgtcgcagg atcgagcacg acccgagagc cgggcgggag ctcgccgagg
      241 agacggttcg ccgccgcgac ccactcgtcg gcctgggcgc ggtcgtcgag gccgatgccc
      301 agcaccgcga tcggcacctc ccgcggcacc aggtggccgg tccgctcgcc gtacaccccc
      361 gcagcgaggt cgatcggctc acccggctcc cagccccaca caccgctcgt accgccgttg
      421 tagttcgccg ggacgcgcac gtcgacgccg cgagcgtcga gcgccctcca cacggccgga
      481 ccaccggaga acgccgcgtc cacgccgtgg aaggccaaga gcacggaccg tccttcgatc
      541 gaggcctcga cctggtcgga cagcacgccc agcgcccgcc agtcgtccgc ggcatcggga
      601 ggtggaagat cgggatccga tgccgcgacg ggcacgagga gcagcccgac caccaggggc
      661 atcgccagca ccatccggac cacggaccgg tcgtcggccg ccgccgccag atcgggtcgc
      721 tgcgctcgca ggtgctcccc ggcgaacgac acgacgccgt acccggtggc gaaccagacg
      781 aacaggccga cggcccacag ccagcggatg taggagatgc ccaccggcgg cgagggcgac
      841 atggccagat ccgcgaacga ggccacgagc acgaccgccg ccgtcgtgag cacggcgaac
      901 cgaccggcgt cggccgcgcg ggtccggatg gcgcccacga gcaccgcgac gaagaggccg
      961 aggaggacca ccgtccacat ccggtccaag acggtgggct tcggcaggtc gaacacgccc
     1021 gtcgcgcgct ggagccacag cggctggagg ctccccagcc gcccgagcat ggcgatggcg
     1081 tacgacggac ccgtctgctc gcggccctgg cccatgctct ggatgagcaa ggtgaggttg
     1141 ccccggccgc tgtcgacctc gttggccacc gggagcagcc acgtcgccgc gatgacgccg
     1201 gtgccggcga gcagcggacc gcgccaccgg cgcagcacgc cgggtccgcc gccgcgccgg
     1261 ctctgccaca gccccgccgc caggcaccag gctccgacca ccagaccgat caggaccgtg
     1321 gagaggtgga cctgggcgat gaacaccgcc aggagcaccg cccacggcag gaaccagcgg
     1381 tggccggcga ccacggccga cgccaccatg aggagcgcga ccagcgggaa gatcgtgatg
     1441 tgcgggttcc agttgtccca cagcagcggc agccccaggc cgaccgagag gacgacgcat
     1501 ccggtcatgt accagccggc tgccgccctc gacagcgtgc gcaccgcagc ccaggcggcg
     1561 agcacgatcg acccgatgtt gacgagggcg acaccgacca gcaaggcgat ggtgcttccg
     1621 ccgccgagga gcgacgggac cgagagcgcc cagaactcca tcggccccag gtggttcacg
     1681 aacggcgccg acccgtcggc ctgctcgacg gggagcgtgg acggcatgcc cacggtgggc
     1741 gtgtgggcgc tgagcacccg gtcgccgttc agggcgatga acgcccggtc accggtgggg
     1801 atccaatggt gggcggccgc ccaccagcag ccgatgacga tgggcgccga ggccatcgcg
     1861 acgtaggcca ccaggagcca gtcgacgcga ccggcgcggc gcggtgttgc gtcctcgtcg
     1921 ggcgtcactg ggctccgcgc tcctcctgat cggcgtctcg ggacgctacc gcctgaggtt
     1981 caggcggtcg tgcaggtagc gtcgcggcca tggtctctcc gcgccgcctc gccgtcatcg
     2041 tcctgctggc cgccgtcgct gcgttcgctg cgggctgcgg tgtcgacccc ggcgatcagg
     2101 cggcctcctc cagctcgtcg ggtccgtccg accgcaccac caccaccgcc ccgagcaagg
     2161 acctctcccc catggagcgg gccatggtcg accgggccaa ggacctctac gtgaagctcg
     2221 ggatggatcc cgacgacgcc gactgcctcg cccgcggact ggtggccgcc ggcgccggcg
     2281 agggccaggt cgacccgacc gacaccgggg cgatgatgga catcgtcaac cagtgcgaca
     2341 tcgacatgag cgagctcaac gacctgggca gcgagaacgg gatcacgtcg atggaagacg
     2401 ggctggagtt cgggctgcag tcgagcctgg agtcgggcgg cctctccaag aaggaggccg
     2461 agtgcgtggc gtcggacttc gtcgaccagt acggcaccga cgtgtcggcg atgcaggacc
     2521 gcgagaaggt cgccgccttg atggagggct gcaacgtcga tccgtcgaag tacggcggct
     2581 gaccgccacc gggctgacgg gcgtcagtcc gcacccgggc ggcggtcggc ctcgtggccg
     2641 gggtcgccct ggtagccggc gggcagcgac cgggccagtt cctcgggcgt cggctcccgg
     2701 tggaggcgcg cctcgtcggg cagcaggtcc accagggcgg ccatgatcgc cttggtgtcg
     2761 gtgtcgggat cggcgaggcc gaggtccacc ggcggaccga cccggatgcg caccgtcggc
     2821 gggtggccga tggccaacac gtcaggggtc cgggcgttgc ggggccacac cttctcggtg
     2881 ccccacagcc cgatcgggat gaccggcgcg ccgctcatcg ccgccagctt ggccgcaccc
     2941 cagcggccct tgagctccgg gtcgaagaac gcccgacccc gcgggatggt gccctgcggc
     3001 atgatcgcca cggcctcgcc cgccgccagc gcctcggcgg ccgccttcag cggctcgtcg
     3061 gaacccgacc cgcgctcgac acggatgccg cccagcgcgg tgaccacctg gccgacgacc
     3121 ggggcgtcga acacctcctt cttgccgagg aagcgcacgg gccggcccgc ttcggccatg
     3181 gcgtacccga tggcgagcgg gtcgaggtac gagcggtggt tgccgacgat gatcgccggc
     3241 ccgtcgggga tgtgctcggc gccctcgacg tcgaggcgca cccacgggaa cagctgcggc
     3301 cgggccagga gct
//
LOCUS       DATMFS010000094         3454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_258590, whole genome shotgun sequence.
ACCESSION   DATMFS010000094 DATMFS010000000
VERSION     DATMFS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258590"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VNQ33_01770"
     CDS             complement(<1..416)
                     /locus_tag="VNQ33_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWJ96853.1"
                     /translation="MAEIELPALGESVTEGTITRWFKAVGDEVAEDEPLFEVSTDKVD
                     SEVPSPVAGYLTEILVEEGDTVDVGTRVAVVSDTADGGGAAAPAAEEPAHEPLPEPLP
                     VSEPEAPAPPAPAAAPEPAAAPAAGSPALLSPVVRRL"
     gene            complement(500..1915)
                     /gene="lpdA"
                     /locus_tag="VNQ33_01775"
     CDS             complement(500..1915)
                     /gene="lpdA"
                     /locus_tag="VNQ33_01775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWJ96854.1"
                     /translation="MSDAPSDSHFDVIVIGGGPAGYGAALYGSAAGLNVALVEKDKVG
                     GTCLHRGCIPAKELLETAHVFRTVSEAARFGVESSAPTLAFATTMARKTEVVTGLFNG
                     LSGLLKSRKVTVFAGTGTLQADRSVLVRSDDGTSSDITGDAVILASGSVPRTIPGFEV
                     DGKTVLTSDEVFELDELPAKVAVIGGGAIGCEFASMLSDLGSEVTILEALPQILPGVD
                     KDVVNMVRRSFKGRSIDVRTGTKVSGHTPGSTGTSIQIEGSDPLNVDAIVVSVGRRPL
                     SESLGLLGTGVVVDDRGFVQVDEYCRTAIDGVWAVGDLIATPGLAHVGYAEAILVIKQ
                     LLGEEAIPVDYAGVAWCIYCQPEVAFAGLSEQAAIDAGYEVVTKKHRWGGNSRAQIIG
                     ELDGVVKVIAEKQADGTAGRVLGVHMAGPWVTEQLGQAYLAVNWEATVDEIAHFIQPH
                     PSLSENFGEAVLALTGRSLNG"
     gene            complement(2018..>3454)
                     /locus_tag="VNQ33_01780"
     CDS             complement(2018..>3454)
                     /locus_tag="VNQ33_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWJ96855.1"
                     /translation="VTEATGNAFINAFAASLKDIAIELFGKDDKAALLIGTAVVAMVI
                     GAGLGMRAIERPLTVVAGFAAFAALGIVMAWRDPLASKPVSVLAAAAAAAVGAGLTIW
                     LARTWQRAGTIEDEQPERGGVLRRTVLIDGGIAAAVALLGVGLARSARHTARATASRV
                     TRKLPRATPKVTVPAGVLDEGAGAVAGISPFITPTDDFYRIDTLFNAPVVDAGTWSLT
                     IKGMVDREITLTYDDLLARDLIEVPITMMCVSNEVGNHLVGTARWTGILLADLLKEAG
                     VQAGAEQVVGQSVDGFTAGFPVEAALDGRDAMLVVGMNGRPLPVEHGFPARLVVPGLY
                     GYVSATKWIEEIRLTTWDEEGFWIPRGWARLGPIKTASRIDVPHDGEALTPGTRAVAG
                     VAWAPHRGIERVEVQVDDGPWRKATLGPTASDDTWVQWWLRWDAPAGEHQIRVRAVDG
                     EGRTQTAARAAPAPDGATGLHTIEVTVG"
BASE COUNT          475 a         1230 c         1256 g          493 t
ORIGIN      
        1 aggcggcgga cgaccggcga cagcagcgcc ggcgaacccg ccgcgggcgc agcggcgggt
       61 tccggcgcag cggcgggagc gggcggggcg ggcgcctcgg gctcagacac cggcagcggc
      121 tcgggcagcg gctcgtgggc cggctcctcg gccgcaggcg cagcagcgcc gccaccgtcg
      181 gcggtgtcgg acacgacggc gacgcgggtg ccgacgtcga cggtgtcgcc ctcctccacc
      241 aggatctcgg tgaggtagcc ggcgacgggc gacgggacct cggagtcgac cttgtcggtg
      301 gacacctcga agagcggctc gtcttcggcg acctcgtcgc cgacggcctt gaaccagcgc
      361 gtgatggtgc cctcggtgac gctctcgccc agggcgggaa gctcgatctc ggccatgcac
      421 cgatctcctt gtgtggtggt tctctgggtt cgttcggggt ggtccgccgg atctcccgta
      481 ggaccgtagc ggggcgggcc tagccgttga ggctgcggcc ggtgagggcc agcacggcct
      541 cgccgaagtt ctccgacagc gacgggtgcg gctggatgaa gtgggcgatc tcgtcgaccg
      601 tggcctccca gttgacggcc aggtaggcct ggccgagctg ctcggtgacc cacgggccgg
      661 ccatgtgcac gccgagcacc cgcccggccg tgccgtcagc ctgcttctcg gcgatgacct
      721 tcaccacgcc gtcgagctca ccgatgatct gggcccggct gttgccgccc cagcggtgct
      781 tcttggtgac gacctcgtag ccggcatcga tcgccgcctg ctcggacagg ccggcgaagg
      841 cgacctcggg ctggcagtag atgcaccagg cgacgccggc gtagtcgacc gggatggcct
      901 cctcgccgag cagctgcttg atcacgagga tcgcctcggc gtagccgacg tgggccagcc
      961 cgggcgtggc gatgaggtcg ccgaccgccc agacgccgtc gatggcggtg cggcagtact
     1021 cgtccacctg cacgaagccg cggtcgtcga ccacgacgcc ggtgccgagc agcccgaggc
     1081 tctccgacag cgggcgccgg ccgacggaca cgacgatggc gtcgacgttg agcgggtccg
     1141 acccttcgat ctggatcgac gtgccggtgg agccgggcgt gtgtccgctg accttggtgc
     1201 cggtgcgcac gtcgatgctg cggcccttga aggaccggcg gaccatgttg acgacgtcct
     1261 tgtcgacgcc ggggaggatc tgcggcaggg cctcgaggat cgtgacctcg ctccccaggt
     1321 cgctcagcat cgacgcgaac tcgcagccga tggcgccgcc gccgatgacc gcgaccttgg
     1381 cgggcagctc gtcgagctcg aacacctcgt cggacgtgag gacggtcttg ccgtcgacct
     1441 cgaagccggg gatcgtgcga ggcaccgagc ccgaggcgag gatcacggcg tcgccggtga
     1501 tgtccgagga cgtgccgtcg tccgagcgga cgaggacgct gcgatccgcc tgcagcgtgc
     1561 ccgtaccggc gaagacggtc accttgcggc tcttcaacag tccggacagg ccgttgaaca
     1621 ggccggtgac gacctcggtc ttgcgggcca tcgtggtggc gaaggcgagg gtcggcgccg
     1681 agctctcgac gccgaacctg gcggcctccg acacggtgcg gaacacgtgg gcggtctcca
     1741 gcagctcctt ggccgggatg cacccgcggt ggaggcaggt gccgccgacc ttgtccttct
     1801 caacgagggc gacgttcaag ccggcggcgg acccgtacag cgcagcgccg tatccggcag
     1861 gtccacctcc gatcacgatc acatcgaagt ggctgtcgga cggggcgtcg ctcacgggga
     1921 tcctctggac aagggggaga tctgttcggc tggcagccta ccgatcagga ccagcggtcc
     1981 gaaccggacg atcctcctgc tccggctggt cgaagcgcta cccgaccgtc acctcgatcg
     2041 tgtgcagccc ggtcgcccca tcgggcgccg gcgcggcgcg cgccgcggtc tgggtccgcc
     2101 cctcgccgtc gaccgcccgg acccggatct ggtgctcacc ggccggtgcg tcccaccgca
     2161 gccaccactg gacccacgtg tcgtcggagg ccgtgggccc gagcgtcgcc ttccgccacg
     2221 ggccgtcgtc cacctgcacc tccacccgct cgatgccgcg gtggggcgcc caggcgaccc
     2281 ccgcgacggc gcgggtgccg ggcgtgagcg cttcgccgtc gtggggcacg tcgatgcgcg
     2341 aggcggtctt gatcgggccc aggcgcgccc agccccgcgg gatccagaag ccctcctcgt
     2401 cccaggtggt cagccggatc tcctcgatcc acttggtggc cgacacgtac ccgtagaggc
     2461 cgggcaccac gaggcgggca gggaacccgt gctccacggg gagcggccgg ccgttcatgc
     2521 cgacgacgag catggcgtcg cggccatcga gcgcggcctc gacggggaac ccggccgtga
     2581 acccgtcgac ggactgcccc accacctgct ccgctcccgc ctggacgccc gcctccttca
     2641 ggaggtcggc gaggaggatg ccggtccagc gggccgtgcc cacgaggtgg ttgccgacct
     2701 cgttcgagac gcacatcatc gtgatgggga cctcgatcag gtcccgggcc agcaggtcgt
     2761 cgtaggtcaa ggtgatctcc cggtcgacca tccccttgat ggtgagggac caggtgccgg
     2821 cgtcgacgac cggggcgttg aagagcgtgt cgatgcggta gaagtcgtcg gtcggcgtga
     2881 tgaacgggct gatcccggcg acggcgccgg cgccctcatc gaggacgccc gccggaaccg
     2941 tgaccttcgg cgtcgcccgc ggcagcttcc gggtcacccg cgacgcggtg gcccgggccg
     3001 tgtgccgggc cgaccgggcg agaccgacac cgaggagcgc cacggcggcg gcgatgccgc
     3061 cgtcgatcag cacggtgcgc cgcagcacgc ccccgcgttc gggctgttcg tcttcgatcg
     3121 tgccagcccg ctgccaggtg cgggcgagcc agatcgtgag cccggcgccg accgcggcgg
     3181 cggccgctgc cgccagcacc gacaccggct tcgacgcgag cgggtcccgc cacgccatca
     3241 cgatgccgag cgcggcgaac gccgcgaacc cggccaccac cgtgagcggc cgctcgatcg
     3301 cccgcatgcc gagccccgct ccgatcacca tggcgacgac cgcggtgccg atgaggagcg
     3361 cggccttgtc gtccttcccg aacagctcga tggcgatgtc cttcagcgat gctgcgaacg
     3421 cgttgatgaa ggcgttgccc gtcgcttcgg tgac
//
LOCUS       DATMFS010000095         5197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_143479, whole genome shotgun sequence.
ACCESSION   DATMFS010000095 DATMFS010000000
VERSION     DATMFS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143479"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1592
                     /locus_tag="VNQ33_01785"
     CDS             30..1592
                     /locus_tag="VNQ33_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWJ96856.1"
                     /translation="MSEPTLGPARLAVLIARLTGVAHEMGEVLRRAAFSPNIKERADC
                     SAALFTADGTLLVQAEHIPVHLGSMPASVAAAIGAFGNGIEPGQQVILNDPFAGGTHL
                     NDVTLVMPVFDGSTIVGWVANRAHHADLGGTAPGSMPADAVHIDEEGLRLSPQILTGE
                     VAAAMAAASRTPEERAGDLDAQVGANRLGAERFGALGTAGVGEIVAYGERRMRAALAD
                     LPDGTWSFTDVLDSFGPAAHQQEPTAIVLTLTVAGDRITFDFTGTDPQREGNVNAVEA
                     VTVSAVAWALRSVLDPTIPANGGALRSVEVVAPAGTVVAARYPAAVGAGNVEVSQRVA
                     DVCLGALAQVVPDRVPAASQGTMNNLLLGGDGWVSYETIAGGQGGRPPRPGSSGPPVP
                     GMSGIHTAMTNSRNTPVEVLERTYPMRVLRYRIREGSGGDGWARGGDGIERDLQVLED
                     VTVSLITERRVSRPWGQNGGEPGAAGENWLLPDGDERRAERLPDKCTLQLEAGDVLRM
                     LTPGGGGWGTRP"
     gene            1992..3107
                     /locus_tag="VNQ33_01790"
     CDS             1992..3107
                     /locus_tag="VNQ33_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96857.1"
                     /translation="MEWLRRIVGGRGQEKSPPALSVPMLSDGEVASQQAQRAERIAEA
                     RRAAFDGGGQLPFREILSGLPADGVVYVGLDHADRLVLATSPSDPDQSYAGRDEAPGW
                     ASFPHSVASESYPVRIEVFDPQGQSTTVATSLPVAYPQLQPTPDGGFLVVGTRARYTS
                     SGPESNAIVLDRTGDAIGSFCIGDGVEHVQVTPSGKIWVGYFDEGVYGNYGWGSPDGP
                     EPLGSAGLVRWSLDGEHEYGFSTAEGPDWIDDCYALSVIDEDAWACYYSDFPVVRVRP
                     DGSVTSWTCGVAGARGLAVLDAERVGLVGGYDGLFDRLVVAELGDGQCTGIRTFQLVT
                     PEGAELPTDARVFARGSSIHALTSGRWLRTDLRDVPA"
     gene            3292..4092
                     /locus_tag="VNQ33_01795"
     CDS             3292..4092
                     /locus_tag="VNQ33_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWJ96858.1"
                     /translation="MDRSVSCRFDARIDETADVVVSVAVADGVPRRTESLAVTLDGAG
                     VAVREVLDQHGTRLHLAPALGPGRIEVTYSASVSGPEPEVPQDEVDLIRYTRPSRYCE
                     SDRLAPFARDEFGGLAGAELVEAVSAWVSARIAYIAGSSRPTDGAVATLLARQGVCRD
                     FAHLVVALLRGCDVPARIASVYAPGLDPMDFHAVAEVLVDGRWVAVDATRLAPRPSLV
                     RIATGRDAADIAFLTTTGGRVDMGWLDVMAITDGDLPSDDHIGFTELG"
     gene            complement(4127..4975)
                     /locus_tag="VNQ33_01800"
     CDS             complement(4127..4975)
                     /locus_tag="VNQ33_01800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWJ96859.1"
                     /translation="MPPSHAARRTLRTAAPVVGVVVALVAAVSLLGSCSGERASLGSL
                     PPKLPAASTTVAAPSTTAAPKPVDLTLGPDDLLGWIATPKGTPEVYAEASTGAKRIDV
                     GAKTEAGAPTTFAIVGDASAGAPRPVPGWYEVALRDRPNGSTGWVPEASVDVTKTPFR
                     IFVGLKDRTLRVEKDGGGVFATTIAIGTDDNPTPVNGTYVTELIENTDPGGSYGPYAF
                     GLAMHSDTLSEFGGGDGQVGIHGTNRPDLIGQAVSHGCVRLKNDDIQDLVDLQLPLGV
                     PVFITA"
BASE COUNT          714 a         1802 c         1884 g          797 t
ORIGIN      
        1 ggcgcgctcg tgatcacgag gaccgctcga tgagcgagcc gaccctgggt ccggcccggc
       61 tcgccgtcct gatcgcccgc ctcaccgggg tcgcccacga gatgggcgag gtgctccgtc
      121 gggccgcgtt cagcccgaac atcaaggagc gggccgactg ctcggccgct ctgttcaccg
      181 cggacggcac gctgctggtc caggcggagc acatcccggt gcacctcggt tcgatgccag
      241 cgtcggtggc cgctgccatc ggggcgttcg gcaacggcat cgaaccgggc cagcaggtca
      301 tcctcaacga cccgttcgcc ggcggcaccc acctgaacga cgtgaccctc gtgatgccgg
      361 tgttcgacgg ttcgaccatc gtcggctggg tcgccaaccg ggcccaccac gccgatctcg
      421 gcggcacggc gccggggtcg atgccagccg acgccgtcca catcgacgag gagggcctcc
      481 gcctctcgcc ccagatcctc accggcgagg tcgcggccgc catggcggcc gcgagccgca
      541 ccccggagga gcgagccggc gacctcgatg cccaggtggg tgccaaccgc ctgggcgccg
      601 agcggtttgg cgcgctgggc accgccgggg tcggtgagat cgtggcctac ggagagcgcc
      661 ggatgcgcgc cgccctggct gacctgcccg acggcacctg gtccttcacc gacgtgctcg
      721 acagcttcgg ccccgccgcc caccagcagg agccgaccgc catcgtcctc acgctgaccg
      781 tcgccggcga ccgcatcacg ttcgacttca ccggcaccga tccccagcgc gagggcaacg
      841 tcaacgccgt cgaggcggtg acggtcagcg ccgtcgcctg ggccctccgc tcggtgctcg
      901 atcccacgat cccggccaac ggcggcgcgc tgcgcagcgt cgaggtcgtc gctcccgccg
      961 gcacggtcgt ggccgcccgg tacccggcgg cggtgggagc gggcaacgtc gaggtcagcc
     1021 agcgcgtggc cgacgtctgc ctcggtgccc tcgcccaggt ggtgccggac cgggtgccag
     1081 ccgcgtccca gggcacgatg aacaacctcc tgctcggtgg cgacggctgg gtctcctacg
     1141 agaccattgc cggcggccag ggcggccgtc cgccgcgtcc cgggagcagc ggtccgccgg
     1201 tcccgggcat gagcggcatc cacaccgcca tgaccaacag ccggaacacc cccgtcgagg
     1261 tgctggagcg gacgtacccg atgcgggtcc tgcgctaccg gatccgagag gggagcggtg
     1321 gcgacggttg ggcccgcggc ggtgacggca tcgagcgaga cctccaggtg ctggaggatg
     1381 tgacggtctc cctcatcacc gagcgccggg tgtcgcgccc ttggggacag aacggtggcg
     1441 agcccggcgc cgctggcgag aactggctgc tgccggacgg tgatgagcgc cgggccgagc
     1501 gcctccccga caagtgcacc ctccagctcg aggctggtga tgtgctccgc atgctgaccc
     1561 cgggcggcgg tgggtggggg actcgaccat gattgcgccg agtgacccgc gggtgtctga
     1621 ggccgtcgtg atctggacgg gcttcggtcg tcaatccttc ccgagcctcg acgagtcccg
     1681 ggtggagcag cgcttcggag acctggcaac ggatctgatc tcgttggttc gaggttggta
     1741 cgacgacttc tacgaatcga acgcgcgaga agtcgccgtg gacctcgttg aggtggggct
     1801 gctcgcctcc gaccggttcc ggtcgcttca tcccgaggct tcagcggagg ccgtcgaggc
     1861 tctggcgtgg tgttacgggg cggggcatca gccggagagc ccgagctcct cggctctggc
     1921 acggaagggc ttgcgagtgc tcgccacgga agttccactg cctgacgttt cttgggccac
     1981 gatgcagggt catggagtgg ttgcggcgga tcgtcggagg tcggggtcag gagaagagcc
     2041 cgccggcact ctcggtgccg atgctgtctg atggcgaagt cgcatcgcag caggcgcaac
     2101 gcgccgaacg catcgcagaa gcacgacgag cggccttcga cggaggtggc cagctcccgt
     2161 tccgcgagat cctgtcgggg ctgccggctg acggcgtcgt ctacgtgggg cttgaccatg
     2221 ctgatcggct cgtgctcgcg acgtcgccgt ccgatcccga tcagtcgtac gcaggacgcg
     2281 acgaggcgcc gggctgggca tcgttccccc acagcgtcgc gagcgagtcg tacccggtca
     2341 ggatcgaggt gttcgacccg caggggcaat cgacaacggt ggcgacatcg ttgccggtcg
     2401 cctatcccca actgcagccg acacccgatg gcggcttcct cgtggtgggc acccgggctc
     2461 gctacaccag cagtggcccc gagtcgaacg ccatcgtgct cgatcgtacc ggcgatgcca
     2521 tcggcagctt ctgcatcgga gacggcgttg agcatgtcca ggtcactccg tcggggaaga
     2581 tctgggtcgg ctacttcgac gaaggggtgt acgggaacta cggctggggg agccccgacg
     2641 gccccgagcc gctcggcagc gcgggcctcg ttcgctggtc gcttgatggg gagcacgagt
     2701 acgggttctc cacggcggaa ggtcctgatt ggatcgatga ctgctacgcg ctcagcgtga
     2761 tcgatgagga cgcgtgggcc tgctactact ccgacttccc cgtggtgcgc gttcgaccgg
     2821 atgggtctgt gacgagctgg acatgtggcg tcgcaggagc gcgtgggctg gccgtgctcg
     2881 acgccgagcg tgtcggcctt gtcggcggat acgacggact cttcgaccgg ttggtcgtcg
     2941 ccgagctcgg cgatgggcag tgcaccggaa tccgaacctt ccagctcgtg accccggagg
     3001 gcgcagagtt gccaacggac gctcgtgtct tcgctcgcgg atcatcgatc cacgcgctga
     3061 cgagcggacg ctggctcagg accgacctgc gcgacgttcc cgcatagcgg cgccgccgag
     3121 gtcgtctcct cacttccaac ttggtgctgc cacctgtcca tcctccaccc acgtgcaccg
     3181 gggtccgggg ccggggtcgt gttggccgag cgccgcacct tggcactggc gtgctccgat
     3241 gccgggcgac gtaccgaaac cggtgggggc ctgacgtggg agcatcgggg catggaccgg
     3301 tccgtcagct gtcgcttcga tgcgcgcatc gacgagacgg cagacgtcgt ggtgtcggtg
     3361 gcggtggcgg acggggtgcc ccgacggacg gagtcgctcg ccgtcaccct cgacggcgct
     3421 ggggtcgccg tgcgagaggt gctcgaccag cacggcaccc ggctccacct ggcgcccgcg
     3481 ctcgggccgg gccggatcga ggtcacctac tcggcctccg tgtcgggccc cgagcccgag
     3541 gtcccccaag acgaggtcga cctcatccgc tacacccggc ccagccgcta ctgcgagtct
     3601 gatcgcctcg ccccgttcgc gcgcgacgag ttcggcggcc tcgcgggcgc cgagctggtg
     3661 gaggccgtgt cggcctgggt gagcgcccgc atcgcctaca tcgccgggtc cagccggccc
     3721 accgacgggg ccgtcgccac gctcctcgcc cggcaggggg tgtgccggga cttcgcccac
     3781 ctggtggtcg ccctgctgcg cggctgcgac gtgccggccc gcatcgcgtc ggtctacgcc
     3841 cccgggctgg acccgatgga cttccacgct gtggccgagg tgctggtcga cgggcgttgg
     3901 gtcgccgtcg acgcgacccg cctggccccg cggccgtcgc tcgtccgcat cgccacaggt
     3961 cgcgacgccg ccgacatcgc cttcctcacc accaccggcg gccgcgtcga catgggttgg
     4021 ctcgacgtga tggccatcac cgacggcgac ctgccgtccg acgaccacat cggcttcacc
     4081 gagctgggct gacccagcgc cgcagatccg tccggccggt tcccggtcag gcggtgatga
     4141 agacggggac gccgaggggg agctggaggt cgacgaggtc ctggatgtcg tcgttcttca
     4201 ggcgcacgca gccgtgggag acggcctggc cgatgaggtc cggccggttg gtcccgtgga
     4261 tgccgacctg gccgtcgccg ccgccgaact cgctgagggt gtcggagtgc atcgccaggc
     4321 cgaaggcgta gggcccgtag gaaccgcccg gatcggtgtt ctcgatcagc tcggtgacgt
     4381 aggtgccgtt gacgggggtc gggttgtcgt cggtgccgat ggcgatggtg gtggcgaaca
     4441 cgccacctcc gtccttctcc acccgcaagg tccggtcctt gaggccgacg aagatccgga
     4501 acggggtctt ggtgacgtcg accgatgctt cgggcaccca gcccgtggag ccgttgggac
     4561 ggtcgcgcag ggccacctcg taccagcctg ggaccgggcg cggtgcgccg gccgaggcgt
     4621 cgccgacgat cgcgaacgtg gtgggggcgc cggcttcggt cttggcgccg acgtcgatgc
     4681 gcttcgcgcc ggtggaggcc tcggcgtaca cctccggcgt gcccttcggc gtggcgatcc
     4741 agccgagcag gtcatcgggg ccgagggtca ggtcgaccgg cttcggtgcg gccgtcgtgg
     4801 atggggctgc gacggtggtg gaagcagccg gcagcttcgg tggcagggac ccgagcgagg
     4861 cccgctcacc cgagcacgat cccagcaggg acaccgcggc gacgagggcg accaccacgc
     4921 cgacgaccgg tgccgcggtc cggagggtgc ggcgggcggc gtgggacgga ggcacgggag
     4981 gcgatggtac tcgtcggtcc atcgccccga tcctcccccc ggcgcagcgc ggtccgattc
     5041 gtcacttgac cgaccagtca atacagttcc cacccatgga gatcaacggc atcagcgcaa
     5101 tcgtcaccgg tggggcatcg ggtctcggag aggccaccgc ccgccacctc agcaccctcg
     5161 gggccaaggt cgtcgtcgcc gacctcgacc gccagca
//
LOCUS       DATMFS010000096         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_73538, whole genome shotgun sequence.
ACCESSION   DATMFS010000096 DATMFS010000000
VERSION     DATMFS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73538"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VNQ33_01805"
     CDS             complement(<1..108)
                     /locus_tag="VNQ33_01805"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HWJ96860.1"
                     /translation="MTEPIRIANCSGFYGDRLSAATEMVEGGPIDVLTGD"
     gene            complement(176..2170)
                     /locus_tag="VNQ33_01810"
     CDS             complement(176..2170)
                     /locus_tag="VNQ33_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWJ96861.1"
                     /translation="MSADPVNPIPTPITSVLVANRGEIARRIIRTARSMGIRTVAVFV
                     EADAGAPYVTEADGAIRIDTRYLDGDAILAAAQASGADAIHPGYGFLSENAAFARAVG
                     DAGITWVGPTPEVIEAMGDKITAKKAAVEAGVPTLPSTEDPAAAGDVGYPLLVKASAG
                     GGGKGMRIVERAEDLDESLAAAQREALSGFGDDRVFLERYVARSRHVEIQILGDQHGT
                     VVHLGERECSIQRRHQKVIEESPSPVVDDAMRAAMGDAALGLARAIGYTSTGTVEFLV
                     DDDTREFFFLEVNTRLQVEHPVTEEVTGIDLVREQFRVAAGEPIEPAAAHATFTGHAI
                     EARLYAEDPAAGFLPAIGTIEAFAPAEEPAVRWDSGVEPGSVIGVDFDPMLAKVVAHA
                     PTRTEAAGRLALALSRLHLGGVTTNRDFLVATLRTPEFLAGDTTTDFIERVGVPLSVE
                     PSPVEHARTAAAAALWLQGRNRAEATVLGAMPSGWRNTRTPAQTASFLIDGNPVSTTY
                     RRLRDGTFEIDGGEAVARIHAWSPTHIDIENAGHRVVVGVTAVGSDLYLQVPRGTVHV
                     QVVPRFTVPGPAEVAGGFVAPMPGVVLDVRVAAGETVTAGQTLVVLEAMKMEHHMAAP
                     ADGTVAEVRVAAGDQVANGALLLVFEPIEPSGSETEEAGS"
     gene            complement(2167..>2654)
                     /locus_tag="VNQ33_01815"
     CDS             complement(2167..>2654)
                     /locus_tag="VNQ33_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ96862.1"
                     /translation="ITGFMVGKDFEEDGIIKKGSQMINAVTNSTVPHLTVVIGASYGA
                     GTYAMSGRAFGNRFTWLWPTAKIAVMGPKQIAGVMSIVRRGQAARKGEAFNEEEDAQL
                     VAMVEAMQEEGSLALRATGAITDDGIIDPRDTRDVLGMCLSVAVNREVAGTEGYGVFR
                     L"
BASE COUNT          346 a          871 c         1044 g          393 t
ORIGIN      
        1 gtcgccggtg aggacgtcga tcggcccgcc ctcgaccatc tcggtggccg cgctcagccg
       61 gtcgccgtag aacccggagc agttggcgat gcggatcggc tcggtcatgc gaacgcccct
      121 tcggtcctgg tcgctgcacg gcgcccagga ggtgccgttc gacgacgagg acggttcacg
      181 acccggcctc ctcggtctcg gacccactgg gttcgatcgg ttcgaagacg aggaggaggg
      241 cgccgttggc gacctggtcg ccggccgcca cccggacctc ggcaacggtg ccgtcggcgg
      301 gggcggccat gtggtgctcc atcttcatgg cctcgaggac cacgagcgtc tggccggcgg
      361 tcaccgtctc gccggcagcg acgcgcacgt cgagcaccac gccgggcatg ggggcgacga
      421 agccgcccgc cacctcggcc ggaccgggca cggtgaaccg agggacgacc tggacgtgga
      481 ccgtgccccg ggggacctgg aggtagaggt cggagcccac ggcggtgacg ccgacgacga
      541 cgcggtgacc ggcgttctcg atgtcgatgt gggtgggcga ccaggcgtgg atgcgggcga
      601 ccgcctcgcc accgtcgatc tcgaacgtgc cgtcgcggag gcggcggtag gtggtgctca
      661 ccgggttgcc gtcgatgagg aaggacgccg tctgcgccgg ggtccgcgtg ttgcgccaac
      721 cgctgggcat ggcgccgagc accgtggcct cggcgcggtt gcggccctgg agccagagcg
      781 cggcggccgc ggcggtgcgg gcgtgctcca cggggctcgg ctccacgctc agcggcacgc
      841 cgacgcgctc gatgaagtcg gtggtcgtgt cgcccgccag gaactccggc gtgcggaggg
      901 tggcgacgag gaagtcgcgg ttggtggtga caccgccgag gtggaggcgg ctgagcgcca
      961 gggcgaggcg gccggcggcc tcggtgcggg tgggggcgtg ggccacgacc ttggcgagca
     1021 tcgggtcgaa gtcgacgccg atgaccgaac cgggctcgac gcccgagtcc caccggacgg
     1081 cgggctcctc ggcgggggcg aacgcctcga tcgtgccgat ggcggggagg aagccggctg
     1141 cggggtcctc ggcgtagagg cgggcctcga tggcgtggcc ggtgaaggtg gcgtgcgcgg
     1201 cggcgggctc gatcggctcg ccggcagcga cgcggaactg ctcgcgcacc aagtcgatgc
     1261 cggtgacctc ctcggtgacc gggtgctcga cttggagacg ggtgttgacc tcgaggaaga
     1321 agaactcgcg ggtgtcgtcg tccaccagga actcgaccgt gcccgtggac gtgtacccga
     1381 tggcccgggc caagccgagg gcggcgtcgc ccatggcggc gcgcatggcg tcgtcgacca
     1441 cgggtgacgg ggactcctcg atgaccttct ggtgcctgcg ctggatggag cactcgcgct
     1501 cgccgaggtg gacgaccgtg ccgtgctggt cgccgaggat ctggatctcg acgtggcggg
     1561 agcgggcgac gtagcgctcg aggaacacgc ggtcgtcgcc gaagccgctg agcgcctcgc
     1621 gctgggcggc ggcgagcgac tcgtcgaggt cctcggcccg ctccacgatg cgcatgccct
     1681 tgccgccgcc gccggctgat gccttcacga gcagcgggta gccgacgtcg ccggccgcgg
     1741 ccgggtcctc agtggagggg agcgtgggca caccggcttc gaccgccgcc ttcttggcgg
     1801 tgatcttgtc gcccatggcc tcgatgacct cgggcgtggg gccgacccac gtgatgccgg
     1861 cgtcgcccac ggcgcgggcg aaggcggcgt tctcggacaa gaagccgtag cccgggtgga
     1921 tggcgtcggc gccgctggcc tgggcggcgg cgaggatggc gtcgccgtcg aggtagcggg
     1981 tgtcgatgcg gatggccccg tcggcctcgg tcacgtacgg ggcgccagcg tcggcttcga
     2041 cgaacacggc gacggtgcgg atgcccatcg agcgggcggt gcggatgatg cgccgggcga
     2101 tctcgccgcg gttggcgacg aggaccgacg tgatgggggt ggggatcggg ttcaccgggt
     2161 cggcgctcac agccggaaca ccccgtatcc ctcggtgccg gcgacttctc ggttgacggc
     2221 gaccgacagg cacatgccga gcacgtcgcg ggtgtctcga gggtcgatga tcccgtcgtc
     2281 ggtgatcgct cccgtggctc ggagggcgag cgagccctcc tcctgcatcg cctccaccat
     2341 ggccacgagc tgggcgtcct cctcctcgtt gaacgcctcg cccttgcggg cggcctggcc
     2401 ccggcggacg atcgacatga cgccggcgat ctgcttgggc cccatcacgg cgatcttggc
     2461 ggtgggccac agccaggtga accggttgcc gaaggcccgg cccgacatag cgtaggtgcc
     2521 ggcgccgtag gacgcgccga tcacgacggt gaggtgcggg accgtcgagt tggtgacggc
     2581 gttgatcatc tggctgccct tcttgatgat cccgtcctcc tcgaagtcct tgccgaccat
     2641 gaagccggtg atgt
//
LOCUS       DATMFS010000097         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_190305, whole genome shotgun sequence.
ACCESSION   DATMFS010000097 DATMFS010000000
VERSION     DATMFS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190305"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VNQ33_01820"
     CDS             <1..222
                     /locus_tag="VNQ33_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HWJ96863.1"
                     /translation="ATMFAWAPIPEPYRELGSIEFASMLVREAQVAVSPGVGFGPGGD
                     GHVRFALIENEQRIGQAIRGLKKALTKLG"
     gene            358..1350
                     /locus_tag="VNQ33_01825"
     CDS             358..1350
                     /locus_tag="VNQ33_01825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ96864.1"
                     /translation="MTPPRLSILDLALIGKGQTARDAFANSVRVARLAEERGYERVWY
                     AEHHNMAQIGSSATSVLIAHVAAHTERIRLGAGGIMLPNHAPLPIAEQFGTLAELHPG
                     RIDLGLGRAPGSDQVTMRAMRRDPTSSDRFPQDVQELQGYLSGDSLVPGVQATPGRGT
                     DVPLYILGSSLFGAKLAAALGLPYAFASHFSPQALRQAVATYRHEFQPSAQQAEPYVI
                     AGVNVIAAETEEDAQAQHLAVRRGRVRMFVQKGIDVSDEQADEILASPQGHQISEMMR
                     YSAVGTPDAVKDYLDGFAVHADADELIVVAASPGIDARLRSLELLADVAGLVSA"
     gene            complement(1465..2064)
                     /locus_tag="VNQ33_01830"
     CDS             complement(1465..2064)
                     /locus_tag="VNQ33_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ96865.1"
                     /translation="MDRKLTQRGEERRRQLMAYATARFAQNGFHPTSVAEITEGLGVG
                     KGVFYWYFDSKEALLRAILADAQLDLRRRQRAAVSGADSPSDGIERGIRASMEWSIQH
                     PDYFRLFQFAATDDRFNGGLRKGEQVAVNDAAKVLAAAMEAGEIARGDATLLSHALLG
                     VHTHLVSLVHRGVLDPTDDVIDAAVDFCLHGLLDRPSPA"
     gene            2183..2845
                     /locus_tag="VNQ33_01835"
     CDS             2183..2845
                     /locus_tag="VNQ33_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWJ96866.1"
                     /translation="MESYVLRVWLPDRPGALGAVASRIGAVKGDVVGIEILETGDGQA
                     VDELVVQLPDATLVDLLVSEVRQVDGVQVEEVRSVGAEGHDPRRATLETAGRLVEAVD
                     RAALLEVARDVAPRSLSAAWIAVIDLEAGALLVAGERSPSESWLVAFVHGSSSAAELQ
                     GSGRGADLGGNDVIWSAMPDVGLAVVAGRDNIAWRTRERHELDALARIVALRWAELPD
                     RG"
     gene            complement(2950..>3884)
                     /locus_tag="VNQ33_01840"
     CDS             complement(2950..>3884)
                     /locus_tag="VNQ33_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWJ96867.1"
                     /translation="LDWSDELCALFGVPRHVLPEVLPSSGRFGVTDESSGLPAGIPIS
                     GIAGDQQAALFGQACFAPGATKNTYGTGSFVLMNVGGTCPEPAEGLLTTVAWSIPDGA
                     GGTRTDYAFEGAVFSTGSAIQWLRDGLGIIASAEEAGPLAASVDTTDDVFFVPAFTGL
                     GSPWWDPYARGTLVGLTRGTTKAHITRAVVESMALQTRDVVDAMCQHAGHPVAGLRAD
                     GGAAVMDLLLQLQADQLGVPVRRPQVQETTALGAAYLAGLAEGVWSSTDDIEANWALD
                     AEFTPAADRGPADALHARWLEAVDRSRGWARSTE"
BASE COUNT          517 a         1353 c         1471 g          543 t
ORIGIN      
        1 gccaccatgt tcgcctgggc gccgatcccc gagccgtacc gggagctggg atccatcgag
       61 ttcgcctcga tgctcgtgcg cgaggcgcag gtggcggtgt cgcccggcgt cggcttcggc
      121 cccggcggcg acggccacgt ccgcttcgcc ctcatcgaga acgaacagcg catcggccaa
      181 gccatccggg gcctcaagaa ggccctcacc aagctcggct gacccaccac ccctccccga
      241 acttcgtagg gcggagcgcc catcggggag ctctcgcata cgaagttcgc tctcggggtg
      301 gggtggggtg gggtgggaat cggacgtggg gtggggaggt tggaacctct tgtgccgttg
      361 actccgcctc gcctgtccat cctcgacctc gccctgatcg gcaagggcca gaccgcccgg
      421 gatgcgttcg ccaacagcgt ccgggtcgcc cggttggcgg aggagcgggg ctacgagcgg
      481 gtctggtacg ccgagcacca caacatggcg cagatcgggt cgtcggccac cagcgtgctg
      541 atcgcccacg tggccgccca caccgagcgg atccgcctcg gcgccggtgg catcatgctg
      601 ccgaaccacg cgccgctgcc catcgccgag cagttcggga cgctggctga gctccacccg
      661 ggtcgcatcg acctcggcct cggccgggcg cccggcagcg accaggtgac gatgcgggcg
      721 atgcgccgcg accccacgtc gtcggaccgg ttcccccagg acgtccagga gctccagggc
      781 tacctgagcg gcgactccct ggtccccggc gtgcaggcga ccccgggtcg ggggacggac
      841 gtgccgctct acatcctggg ctcctcgctc ttcggcgcga agctcgccgc cgccctcggc
      901 ctgccctacg cgttcgcatc gcacttctcc ccgcaggcgc tgcggcaggc cgtcgccacg
      961 taccgccacg agttccagcc gtcggcgcag caggccgagc cctacgtgat cgccggcgtg
     1021 aacgtcatcg ccgccgagac ggaggaggac gcccaggccc agcacctcgc cgtgcggcgg
     1081 ggccgggtgc ggatgttcgt gcagaagggc atcgacgtgt ccgacgagca ggccgacgag
     1141 atcctggcct ccccgcaggg ccaccagatc tcggagatga tgcggtactc ggccgtcggc
     1201 acgcccgacg cggtgaagga ctacctcgac ggcttcgccg tccacgccga tgccgacgag
     1261 ctgatcgtgg tcgcggcatc gcccggcatc gacgcccgtc tccgctcgct cgagctcctc
     1321 gccgacgtcg ccggcctcgt ctcggcctga ccctcgacga gcgtccgccg gctccaaccc
     1381 cgctccatgc gggtgggtgg tcgagggggg cggggtctcg gtccgcgtcg atccgccatc
     1441 ggcgggcccg gacgcccggc ccggctaagc gggggagggg cggtcgagca gaccgtggag
     1501 gcagaagtcc acggccgcat cgatgacgtc gtcggtgggg tcgaggacac cccggtgcac
     1561 gaggctcacg aggtgggtgt gcacgccgag gagcgcgtgc gacaggagcg tggcgtcgcc
     1621 tcgggcgatc tcaccggcct ccatggcggc ggcgaggacc ttggcggcgt cgttcaccgc
     1681 gacctgctcg cctttgcgca gcccgccgtt gaagcggtcg tccgtggcgg cgaactggaa
     1741 caggcggaag tagtccgggt gctggatcga ccactccatc gaggcgcgga tgccgcgctc
     1801 gatgccgtcg gagggggagt cggcgccgct caccgcggcg cgctgccgtc ggcgcaggtc
     1861 gagctgcgcg tcggcgagga tcgcccggag cagcgcttct ttcgagtcga agtaccagta
     1921 gaagacgccc ttgccgacgc cgaggccctc ggtgatctcg gcgaccgacg tcggatggaa
     1981 cccgttctgc gcgaagcggg cggtggcgta cgccatgagc tggcggcgcc gctcttcgcc
     2041 ccgttgggtc agcttccggt ccatcacccg gtcaacctac ttgaccgcct ggtcaagcag
     2101 ccgggacccg gcctcggccc cgcctcggag tcctcccgcg gacgaaacag tccgttcaca
     2161 tcgagcacct agggtcggcg gcatggagag ctacgtgctg cgcgtctggc tgccggaccg
     2221 ccccggtgcg ctgggcgcgg tggccagtcg gatcggcgcc gtgaagggcg atgtggtcgg
     2281 gatcgagatc ctcgagaccg gtgacggcca ggcggtggac gagctggtgg tccagctgcc
     2341 cgacgccacg ctggtcgacc tgctggtgag cgaggtgcgc caggtcgacg gcgtgcaggt
     2401 ggaggaggtc cgttcggtgg gcgccgaggg ccacgacccc cggcgcgcca cgctggagac
     2461 ggccggccgg ctggtcgagg cggtcgaccg ggccgcgctg ctcgaggtgg cccgagacgt
     2521 cgccccgcgg tcgctgtcgg ccgcgtggat cgccgtcatc gacctcgagg ccggcgccct
     2581 gctcgtcgcg ggcgagcggt cgccgagcga gagctggctg gtggcgttcg tccacgggtc
     2641 gagcagcgcc gccgagctgc agggctcggg ccgcggcgcc gacctgggcg gcaacgacgt
     2701 gatctggtcg gcgatgcccg acgtcggcct cgccgtcgtc gccgggcgcg acaacatcgc
     2761 gtggcgcacc cgggagcgcc acgagctcga cgccctcgcc cgcatcgtcg ccctccgctg
     2821 ggccgagctg ccggatcggg gctgagggcc gagggccgag ggaacgaagt tggtgtgcga
     2881 atcgagggag aaggtcgttt cccacaccaa cttcggttcg ctgccgctcg gttgcggtcg
     2941 gaacgaggcc tactcggtgc ttcgtgccca gccgcgtgag cggtcgacgg cttcgagcca
     3001 gcgggcgtgg agggcatcgg ccggcccgcg gtcggcggcc ggggtgaact cggcgtcgag
     3061 ggcccagttg gcctcgatgt cgtcggtgga ggaccagacg ccctcggcca gcccggccag
     3121 gtacgcggcg ccgagggccg tcgtctcctg gacctgtggg cggcggaccg ggacgccgag
     3181 ctgatcggcc tggagctgca ggagcaggtc catcacggcc gcacctccgt cggcgcggag
     3241 cccggcgacg gggtggccgg cgtgctggca catggcgtcg accacgtcgc gggtctggag
     3301 ggccatcgac tcgacgacgg ctcgggtgat gtgggccttg gtggtgccgc gcgtgaggcc
     3361 gacgagggtg ccccgggcgt acgggtccca ccacgggctg ccgagcccgg tgaaggccgg
     3421 cacgaagaac acgtcgtcgg tggtgtcgac cgatgcggcg agcggcccgg cctcctcggc
     3481 gctggcgatg atgccgagtc cgtcgcgcag ccactggatg gccgagccgg tcgagaagac
     3541 ggcgccctcg aacgcgtagt cggtgcgcgt gcccccagcc ccgtcgggga tgctccaggc
     3601 gaccgtcgtg aggaggccct cagccggctc cgggcaggtg ccgcccacgt tcatcagcac
     3661 gaaggaaccg gtcccgtagg tgttcttggt ggcgccgggc gcgaagcacg cctggccgaa
     3721 cagcgcggcc tgctggtcgc cggcgatgcc gctgatgggg atgccggccg gaaggcccga
     3781 ggactcgtcg gtgacgccga agcggccgct ggagggcagc acctcgggca gcacgtggcg
     3841 ggggacgccg aagagggcgc acagctcgtc ggaccagtcg agcg
//
LOCUS       DATMFS010000098         2643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_306051, whole genome shotgun sequence.
ACCESSION   DATMFS010000098 DATMFS010000000
VERSION     DATMFS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306051"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VNQ33_01845"
     CDS             <1..691
                     /locus_tag="VNQ33_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWJ96868.1"
                     /translation="VELTIAVMAAFGATVDQPDERTFAVAPGGYTATDYAIEPDASAA
                     SYPLAAAALCGGRVTVTGLGDGALQGDAAFADVLAEMGAAVTRDDRGTTVTAGRGALR
                     GGTFDFTHISDTAQTLAAIAPFASSPVTITGIGFIRRKEIDRVAAVATELRRAGVRVD
                     DDPDGWTIHPGPVAPATFETYDDHRMAMSFALLGLAAPGISIAGPTCVAKTFPGYWAM
                     LDQLRASATDR"
     gene            703..1368
                     /gene="cmk"
                     /locus_tag="VNQ33_01850"
     CDS             703..1368
                     /gene="cmk"
                     /locus_tag="VNQ33_01850"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWJ96869.1"
                     /translation="MSDAETTDQPLTVIAIDGPAGSGKSTVGKRLAAQLGLEYLDTGA
                     MYRGVTFAALRRGIDPADTEVVARLAQQVELDLAADGTLTVDGVDATIEIRGPEVTRA
                     VSLVAANSEVRKELVRRQREWAEVRGGGVLEGRDIGSVVFPDAKLKVYLTASPEVRAE
                     RRSKEVTDLDYATVAADIARRDAADQGRDDSPLAEAEGALVVDTSDRAIDEIVADLVS
                     RIP"
     gene            1365..2060
                     /locus_tag="VNQ33_01855"
     CDS             1365..2060
                     /locus_tag="VNQ33_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWJ96870.1"
                     /translation="MSSPSEHARAATRGELLFYAVVRAVLLGACKLWFRVSVKDRQKV
                     PAEGAFILAPVHRSNLDFALVLVCTKRRMRYLAKDSLWKGQGFWAKVFTALGGIPVAR
                     GTADREALRTCIEVIEAGEPLVIFPEGTRQHGPVVEELFDGPAFVQSRTGVPIIPVGI
                     GGSEAAMPKGSKIPKPHRVTVVVGDALPFAEHEGSKAKRAAIRARTAELHEVVQALFD
                     QAQSTAGTPNPAS"
     gene            complement(2112..>2643)
                     /locus_tag="VNQ33_01860"
     CDS             complement(2112..>2643)
                     /locus_tag="VNQ33_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWJ96871.1"
                     /translation="TLSHRDWEGVLDATRERFPDLWTPGRSDLCFATTNRQGALAEIA
                     PRCDAVVVIGSANSSNTVALEKLAREAGTARVHRINSAAELPDDLTGVVGVTAGASAP
                     ESLVDEVIAALAPVHGVEEVAITQEDEYFPTPRNLRDLLSGIDLLATLSLGGSVPDRP
                     AADERSIHAAGVLASL"
BASE COUNT          364 a          974 c          947 g          358 t
ORIGIN      
        1 cgtcgagctc acgatcgccg tgatggctgc cttcggagcc acggtcgacc agcccgacga
       61 gcgcaccttc gctgtcgctc ccggcggcta caccgccacc gactacgcca tcgagcccga
      121 tgccagcgcc gcgtcgtacc cgctggccgc ggccgccctc tgcggtggcc gggtgaccgt
      181 caccgggctg ggcgacggtg cgctccaggg cgatgcggcc ttcgccgatg tcttggccga
      241 gatgggtgcc gccgtcaccc gtgacgaccg tggcaccacc gtgacggccg gtcggggcgc
      301 actccggggc gggacgttcg acttcaccca catctccgac acggcgcaga ccctggctgc
      361 catcgccccg ttcgccagct cgccggtcac catcacgggc atcgggttca tccggcgcaa
      421 ggagatcgac cgcgtcgccg ccgtcgccac cgagctgcgg cgggccggcg tgcgcgtcga
      481 cgacgatccc gacggctgga ccatccaccc cggtcccgtc gcgcccgcca cgttcgagac
      541 ctacgacgac caccgcatgg cgatgagctt cgccctgctg ggcctcgccg cgcccgggat
      601 ctcgatcgcc ggcccgacct gcgtcgccaa gacctttccc gggtactggg cgatgctcga
      661 ccagcttcga gcctccgcga ccgaccgata gggtctgctc ccgtgtccga cgccgaaacc
      721 accgaccagc cgctgaccgt catcgccatc gacgggcccg ccgggtccgg caagtccacc
      781 gtcggcaagc gcctcgccgc ccagctcggg ctcgagtacc tcgacaccgg cgccatgtac
      841 cggggcgtga cgttcgcggc gctgcggcgg gggatcgacc cagccgacac cgaggtcgtg
      901 gcccgcctcg cccagcaggt cgagctggac ctggcggccg acggcaccct caccgtggac
      961 ggcgtcgacg ccaccatcga gatccgcggc cccgaggtca cccgggcggt gtccctggtg
     1021 gccgccaact ccgaggtccg caaggagctg gtccgccgcc agcgggagtg ggccgaggtc
     1081 cgcggcgggg gcgtgctcga gggacgagac atcggctcgg tcgtgttccc cgacgccaag
     1141 ctcaaggtgt acctgaccgc ctcgccggag gtccgagccg agcgccggtc caaggaggtc
     1201 accgacctcg actacgcgac cgtcgccgcc gacatcgccc gccgcgacgc cgccgaccaa
     1261 ggccgcgacg acagcccgct ggccgaggcc gaaggcgcac tggtggtgga caccagcgac
     1321 cgggcgatcg acgagatcgt cgccgacctc gtgagccgca tcccgtgagt tcaccgtccg
     1381 agcacgcgcg ggccgccacc cgcggcgagc tgctcttcta cgccgtcgtc cgggcggtcc
     1441 tgctcggcgc ctgcaagctg tggttccggg tctcggtcaa ggatcgtcag aaggtgcccg
     1501 ccgagggggc gttcatcttg gccccggtcc accgctcgaa ccttgacttc gcgctcgtct
     1561 tggtgtgcac caagcgccgc atgcgctact tggccaagga ctcgctctgg aagggccagg
     1621 ggttctgggc caaggtcttc acggcgctgg gcggcatccc ggtcgcccgc ggcacggcgg
     1681 accgggaggc gttgcggacc tgcatcgagg tgatcgaggc cggcgagccg ctcgtgatct
     1741 tcccggaggg cacccgccag cacggaccgg tcgtcgagga gctgttcgac gggccggcgt
     1801 tcgtccagag ccgcaccggg gtcccgatca tccccgtcgg catcggcggc tccgaggcgg
     1861 ccatgccgaa gggctccaag atcccgaagc cccaccgggt caccgtggtg gtgggtgacg
     1921 ccctcccgtt cgccgagcac gagggctcga aggccaagcg ggcagccatc cgggcgcgca
     1981 cggcggagct ccacgaggtc gtgcaggccc tcttcgacca agcccagtcc accgcgggga
     2041 ctcccaaccc ggcgtcttga ccaggtcctg cctccgggcg gcggggtcgg caccgaaggc
     2101 gcgtcgcgcg ctcagagcga ggcgaggacg ccggcggcgt ggatgctgcg ctcgtcggcg
     2161 gcggggcggt cggggaccga accgcccagg ctcaaggtgg ccaggagatc gatgcccgag
     2221 agcaggtccc ggaggttgcg gggcgtgggg aagtactcgt cttcctgcgt gatggcgacc
     2281 tcctcgaccc cgtggaccgg ggcgagcgcg gcgatgacct cgtcgacgag cgattcggga
     2341 gccgacgcac cggccgtgac cccgaccacc ccggtgaggt cgtcggggag ctcggcggcg
     2401 gagttgatgc ggtgcacgcg cgcggtgcct gcctcccgcg ccagcttctc gagcgccacc
     2461 gtgttcgacg agttggccga gccgatgacg acgacggcat cgcagcgggg agcgatctcg
     2521 gcgagggcgc cctgccggtt ggtggtcgcg aagcacaggt cggagcggcc aggggtccac
     2581 aggtcgggga agcgctcgcg ggtggcgtcg agcacgccct cccagtcgcg gtggctgagc
     2641 gtg
//
LOCUS       DATMFS010000099         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_4722, whole genome shotgun sequence.
ACCESSION   DATMFS010000099 DATMFS010000000
VERSION     DATMFS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4722"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            881..1951
                     /locus_tag="VNQ33_01865"
     CDS             881..1951
                     /locus_tag="VNQ33_01865"
                     /inference="COORDINATES: protein motif:HMM:NF022470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HWJ96872.1"
                     /translation="MAWDLLVDRSDLSNTTLVEVPTPEPGAGQVLLRSDRVGMTANNV
                     TYAVFGDAMKYWDFFPAADVDGKPQGRVPLWGFAVVEASTVAEVPEGTRLYGYLPTSS
                     HLIVEPAKADERGFRDGSRHRQHLPAPYNRMTTTAEDPAYDAGLEDLQVLYRPLFMTS
                     FMLADFIDDHDRFGADAVVISSASSKTSYGTAFLLDGIHRIGLTSEGNRAFTESLGCY
                     DEVRTYGEVDDLDPVKAVYVDVAGDGALRRRVHERLEPVHSAVVGAAHHDAAPDLGTD
                     DLPGGRPTFFFAPDQMRKRYEDWGPDGVEERHAAAWARFAPVVAGWVDVVTSTGPEGL
                     RTAWLETLAGTTSPRTGLVIAP"
     gene            2225..2935
                     /locus_tag="VNQ33_01870"
     CDS             2225..2935
                     /locus_tag="VNQ33_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HWJ96873.1"
                     /translation="MRTTRLAVAAAVAAVLVAVFAGPASAHVTIPDPGKKGGFSIVTF
                     SVPNERDDASTTKIEVQLPKDEPLAFVSVQPKPGWEVETTKRTLDKPIEAFGETYDEV
                     VDTVTWSGGKIAPGEFDTFAISVGPLPEDVDSLTFPTIQTYSSGEEVSWIDVADEGAE
                     EPEHPAPTLTLVAADDGGHGSEATTTTAASSASGEAAASSDDGGTDGVAVAALVVAVV
                     AAVLGAAALVVGRRSSAA"
     gene            complement(2984..3466)
                     /locus_tag="VNQ33_01875"
     CDS             complement(2984..3466)
                     /locus_tag="VNQ33_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ96874.1"
                     /translation="MTELHLTPRPTVDILGNLPAGQRALYRLEQAIAESPLPKSTYEL
                     IKIRASQINGCAFCLEMHHHDAVEMGETATRLALLPAWRESGLYTDEERLALALTEAV
                     TRIADGEVPVELEAEARKVLGEEAYAAVIFAIVAINSWNRLAIVGHKVPGSLREGAAS
                     "
BASE COUNT          501 a         1225 c         1272 g          540 t
ORIGIN      
        1 tccgttccgt cggtcctcgt gggatcaaca cagggaccaa cgagggatgg atcggccggg
       61 ggaggtggtc gtggtcggca cgtgcgatca ccgcatcgag gtcaggcgaa gggttgtccc
      121 atcgagacat gggcggcgct cctgctcgat cgggccgcgg ccaggggcaa ggctggcgcg
      181 gacaaggtgt ctgtgcgacg acctgtcgcc agggtcgttc ctgtcggctc gtccagggaa
      241 ggagaccccc gctcgcgcgg gccacggcgt gcgagatcgg ccaggtcggc gttggtcgct
      301 cgattctggt ggaacgcggt ccactccgcc tcgccgttgt cgtcggccga ggtcgatccc
      361 cgaagggaca cggtctcccg tggctcgtcg ccgccgtcca tcctcgagac ggtactggtg
      421 ctgcgggcga gaagcctggg gcgctcttgg cctctcagca gggagggggg ttgatcctcg
      481 tatcggcgag atccactcgc agatcgcgcc ggtgaccgcc gcccgctggt cggcgttccg
      541 ggcgcgctcc gcggcgtgtg agttccgtgg ctggtgggaa tcgtgtgcga gcgatgcgcc
      601 gagcattggg agcgggatcg caaggagctc atgatctgtt ctcaggcaac gccgggcgtt
      661 cgtgccatca aggtcgtgca acgtgcaggc gcccagtagg cacgcgccgt cctccgggct
      721 gttccccggt ctggcggagc acgccggcgc attccatccg ccggtgctca gccgtgccgg
      781 ggtgtcagct gaacgtggct tcgttggact gatccctgcg tccgccgtgc cggccccact
      841 cgctccgcat cgcagtacga gaccgcggta gaccgttcgc atggcttggg acttgctcgt
      901 cgaccgttcg gacctctcca acaccaccct cgtggaggtg ccgacgccgg aaccgggggc
      961 ggggcaggtg ctgcttcggt ctgaccgggt ggggatgacg gcgaacaacg tgacctatgc
     1021 cgtcttcggc gatgcgatga agtactggga cttcttcccc gcggccgatg tcgacgggaa
     1081 gccgcagggg cgggtgccgc tgtgggggtt cgccgtcgtc gaggcgagca ccgtcgccga
     1141 ggtccccgag ggcacccggc tctacgggta cctgccgacg agcagccacc tgatcgtgga
     1201 gccggcgaag gccgacgagc gcgggttccg cgacggcagc cggcaccgcc agcacctgcc
     1261 ggcgccgtac aaccggatga cgaccacggc cgaggacccg gcgtacgacg ccgggctcga
     1321 ggacctccag gtcctgtacc ggccgctgtt catgaccagc ttcatgctgg ccgacttcat
     1381 cgacgaccac gaccggttcg gggccgatgc cgtggtgatc agcagtgcgt cgagcaagac
     1441 gtcgtacggc acggcgttcc tcctcgacgg catccaccgc atcggcctca ccagcgaggg
     1501 caaccgggcg ttcaccgagt cgctcggctg ctacgacgag gtccgcacct acggcgaggt
     1561 cgacgacctg gacccggtga aggcggtgta cgtcgacgtc gccggtgacg gggccctgcg
     1621 ccgccgggtc cacgagcgcc tggagccggt ccactcggcg gtggtcggcg ccgcccacca
     1681 cgatgccgcc ccggacctcg gcaccgacga cctgcccggc ggccgcccca cgttcttctt
     1741 cgcccccgac cagatgcgca agcgctacga ggactggggt cccgacggtg tcgaggagcg
     1801 ccacgccgcg gcctgggccc gcttcgcccc cgtcgtcgcc ggctgggtcg acgtcgtcac
     1861 cagcaccggc ccggaaggcc tccgaaccgc gtggcttgag accctcgccg gcaccacctc
     1921 cccgcgcacc ggcctcgtca tcgcgccctg accctgcgca gccaccccgt ctccgagccg
     1981 cagccggagc cggagccggt tactggtcgc tggtcgtgtg ccgagtccgg agagcggttg
     2041 cggtccctcg cccggctgca cgcggatggc ctggtctccg acgaggatct cgccgccgag
     2101 cggaccccag ctgctcgacc tgctcgacgc gggcctccag gcggatctgg gaaccatccg
     2161 gtctgtcgtg ccgacgtatg gacgccccgg gaaaccgccc gggctgtccc caggagcaac
     2221 tcccttgcgc accacccgtc tcgctgttgc tgcggctgtc gccgcagtcc tcgtcgccgt
     2281 gttcgccggg ccggcgtcgg cccacgtcac gatccccgac ccggggaaga agggcgggtt
     2341 ctcgatcgtg accttcagcg tgccgaacga gcgggacgac gcgtccacca cgaagatcga
     2401 ggtccagctg ccgaaggacg agccgctcgc cttcgtgtcc gtccagccca agcccgggtg
     2461 ggaggtcgag acgaccaagc gcacgctcga caagccgatc gaggcattcg gcgagaccta
     2521 cgacgaggtc gtcgacaccg tcacctggtc gggcggcaag atcgcccccg gtgagttcga
     2581 cacgttcgcc atctccgtgg gcccgctccc cgaggatgtg gacagcctga cgttcccgac
     2641 catccagacg tacagcagcg gcgaagaggt gtcttggatc gacgtggccg acgagggggc
     2701 cgaggaaccc gagcacccgg ccccgaccct gacgctcgtc gcggccgacg atggcggcca
     2761 cggcagcgag gccaccacca cgaccgcggc gtcgagcgcg agcggcgaag cagccgcgag
     2821 ctccgacgac ggcgggaccg acggggtggc cgtggcggcc ctcgtggtcg cggtggtcgc
     2881 ggccgtgctg ggcgccgccg ccttggtggt gggccgccgg tcgagcgctg cttgatcccg
     2941 agccggtgcg gtcggagccg gcctccgacc gcaccggggt gggtcagctc gccgcgccct
     3001 cgcgcagcga gccagggacc ttgtggccga cgatggcgag gcggttccac gagttgatgg
     3061 cgacgatggc gaagatgacc gcggcgtagg cctcctcgcc gaggaccttg cgggcctcgg
     3121 cttcgagctc cacggggacc tcgccgtcgg cgatgcgggt cacggcctcg gtgagggcga
     3181 gggccaggcg ctcctcgtcg gtgtagagcc cgctctcccg ccatgccggg aggagagcga
     3241 gccgggtggc ggtctcgccc atctcgacgg cgtcgtggtg gtgcatctcc aggcagaagg
     3301 cgcagccgtt gatctgcgac gcccggatct tgatcagctc gtaggtcgac ttcggcagcg
     3361 gggactcggc gatggcctgc tccagccggt acagcgcccg ctggccggca gggaggttgc
     3421 cgaggatgtc cacggtgggg cggggcgtga ggtgcagctc ggtcatggga tgctccgatc
     3481 aatctggata cataatgtcc agatcgttct accacaggtc accgcagaaa cctggatg
//
LOCUS       DATMFS010000100         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_166505, whole genome shotgun sequence.
ACCESSION   DATMFS010000100 DATMFS010000000
VERSION     DATMFS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166505"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNQ33_01880"
     CDS             <1..578
                     /locus_tag="VNQ33_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWJ96875.1"
                     /translation="VIGLLGVVDQLDKRPPGVTMADGDALVLLGDDLRATSPSLAGSA
                     VAWAAGEHGGTLPAFDGPAHTRVADLVRDLANEGALSGVHDASSGGLGAALAELTCRT
                     GLGLRVDAPVNAAQLFCESPSRVVAAVAASEAEALVARAAGAGVPAVVLGHAGGDRLV
                     LGEVVDLAADEVVSTWRDRLPVALGAGTTQA"
     gene            759..>2003
                     /locus_tag="VNQ33_01885"
     CDS             759..>2003
                     /locus_tag="VNQ33_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96876.1"
                     /translation="MRRSIRLAILAAVAGVGLPVVASQTAVSAVDQDLVLSAASGAPG
                     DEIEVTSASCDTSNQGDTLRYLYVRLVAGTAPDAMLAGTGFGIDDEPARLVLPDWTTP
                     GTAAIEASCVEIAFPADDNGEPVVTQSAYDPVAFDLLAGAGTPVQTRVYGRTSLQAGQ
                     AFSVTASGCFLDAADHAYIDIVPGDDLAFSELTEVGFTGEGEMDGDSFEALAAMSNGT
                     IDLSITGDGSGPEDIAIHEIPTDIPAGTYSTVAYCTREDGATLMFEPQAIEITGDAPF
                     GDIDLTTAAATPDATLAGGSCTAGDVSALLFAEDLDELFFDPGNVARTDAGPRLSPAG
                     FVLDRDDHNARGGSLAGAGARTRLIDTDSVDALATPAADGSWSLTDQVAYDRALIGAY
                     AACGDPLGDGFIYDPQAAVLALA"
BASE COUNT          255 a          780 c          688 g          280 t
ORIGIN      
        1 ccgtcatcgg cctgctcggc gtcgtggacc agctcgacaa gcgcccgccc ggggtcacca
       61 tggccgacgg cgacgcgctc gtcctgctgg gcgacgacct gcgtgccacc tccccgtcgc
      121 tggccggctc ggctgtcgcc tgggcggcgg gcgagcacgg cggcaccctc ccggcgttcg
      181 acgggccggc ccacacccgc gtcgccgacc tcgtccggga tctggcgaac gagggtgcgc
      241 tgagcggcgt ccacgacgca tcgtccggag gtctgggtgc ggccctggcc gagctgacct
      301 gccggaccgg cctcggcctg cgggtcgatg cgccggtcaa cgcggcccag ctgttctgcg
      361 agtctccctc gcgggtggtg gccgcggtcg ccgccagcga ggccgaagcc ctggtggccc
      421 gagcggccgg cgcgggtgtc ccggcggtcg tcttgggcca cgccggtggc gaccgcctgg
      481 tcctgggcga ggtggtcgac ctcgctgctg acgaggtcgt cagcacctgg cgggatcgcc
      541 tcccggtcgc cctcggcgcc ggcaccaccc aggcctgacc gggccgcgcc gcgctctgcc
      601 cggtgacctg tcgccgacct gcaacgcgtt cgcaatgcac gcagggtgag ttctgtggca
      661 cgctgtgcgc gttcggcggg gatctcgagc ggcgcagcag tgcggcacgc ggtgaccagc
      721 ccacacctcg ttcgccccgt ccacgaaagc ttccgcccgt gcgccgttcc atccgtctcg
      781 ccatcctcgc tgccgtcgct ggcgtcggcc tgcccgtcgt cgccagccag accgcggtct
      841 cggccgtcga ccaggacctg gtcctctcgg cggcgtcggg tgctcccggt gacgagatcg
      901 aggtgacctc ggccagctgc gacaccagca accagggcga cacgctgcgg tacctctacg
      961 tccgactggt cgctggcacg gcaccggatg cgatgctcgc cggtaccggc ttcggcatcg
     1021 atgacgagcc ggcgcgcctc gtgctgcccg actggaccac ccccgggacg gccgccatcg
     1081 aagcctcctg cgtcgagatc gccttcccgg cggacgacaa cggggagccc gtcgtgaccc
     1141 agtccgcgta cgacccggtc gccttcgacc tgctggccgg tgccggcacg ccggtgcaga
     1201 cccgggtcta cggccggaca tcgctccagg ccggccaggc gttctcggtc acggcgtccg
     1261 gctgcttcct cgacgccgcc gaccacgcct acatcgacat cgtgccgggc gatgacctgg
     1321 ccttcagcga gctgaccgaa gtcgggttca ccggcgaagg cgagatggac ggcgattcgt
     1381 tcgaggcgct ggccgcgatg agcaacggca ccatcgacct cagcatcacg ggcgacggct
     1441 cggggcccga ggacatcgcc atccacgaga tcccgaccga catcccggcg ggcacctaca
     1501 gcacggtcgc ctactgcacc cgggaggacg gcgccacgct catgttcgag ccgcaggcca
     1561 tcgagatcac gggcgacgca ccgttcggcg acatcgacct gaccacggct gcggccacgc
     1621 ccgacgcgac gctcgccggc ggctcctgca ccgcaggcga cgtgagcgca ctgctgttcg
     1681 ccgaggacct cgatgagctg ttcttcgatc cgggcaacgt cgcccgcacc gacgccggcc
     1741 cccgcttgag cccggccggc ttcgtgctcg accgagacga ccacaacgcg cgaggggggt
     1801 ccctcgccgg cgccggcgcc cggacccggc tcatcgacac cgactcggtc gacgccctgg
     1861 ccaccccggc agccgacggc tcctggtcgc tgaccgacca ggtcgcctac gaccgggcgc
     1921 tgatcggggc ctacgccgcc tgcggcgacc cgctcggcga cggcttcatc tacgacccgc
     1981 aagccgccgt gctggcgctg gcc
//
LOCUS       DATMFS010000101         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_143719, whole genome shotgun sequence.
ACCESSION   DATMFS010000101 DATMFS010000000
VERSION     DATMFS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143719"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VNQ33_01890"
     CDS             <1..329
                     /locus_tag="VNQ33_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWJ96877.1"
                     /translation="PEMQAKDAIAQYWGGNAKCAERIAQRESGMQPDAVNRSSGATGL
                     FQLMPLHKTWIKNELGYDFSEMKDPYKNAEAAKLLSDKNYKAYKDGWAPWRLSGRADP
                     GGGCPA"
     gene            538..1353
                     /locus_tag="VNQ33_01895"
     CDS             538..1353
                     /locus_tag="VNQ33_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ96878.1"
                     /translation="MTTEPDRAEAPVLVHDEARVRTLTLNRPGALNACNEALYDALAN
                     GLLEAAADPNVAVVLLTGAGRAFCAGTDLVEMAARVMEPDDFVEGEHGFLGLIDALTD
                     FPKPLVIAVNGLGLGIGATILGFADLVFMSTAAKLKCPFTSLAVAPEAASSLLFPQLV
                     GRQNATWMLLSSEWISAAEAKEMGLVWKLTEPDELLGAARQHAEHLAALPISSLVACK
                     RVITEPLRPQIEAARQRENDAFVELLGSPANLEAFAAFAEGRKPNFVDLPGGG"
     gene            1564..>2029
                     /locus_tag="VNQ33_01900"
     CDS             1564..>2029
                     /locus_tag="VNQ33_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWJ96879.1"
                     /translation="MDQAGDALATVAPALAFLLVGVPLAALLDGLGFFDSVAGLLAER
                     FGQIPVLALWALAAATTVALNLDTTVVLLTPLYVRLARRSGVDPVPLALIPLLLAAFA
                     SSVLPISNLTTLIAAEQLDLSVGDVLGHLGLPSLAAASIGWIAYRRIHPTRLA"
BASE COUNT          278 a          760 c          681 g          310 t
ORIGIN      
        1 cgcctgagat gcaggcgaag gacgccatcg cccagtactg gggtggcaac gccaagtgcg
       61 ccgagcggat cgctcagcgc gagtccggca tgcagcccga cgccgtgaac cgcagcagcg
      121 gtgccaccgg cctcttccag ctgatgccgc tccacaagac gtggatcaag aacgagctcg
      181 ggtacgactt ctccgagatg aaggacccgt acaagaacgc cgaggctgcc aagctcctct
      241 cggacaagaa ctacaaggcc tacaaggacg gctgggctcc ctggcgcctc agcggccggg
      301 cggatcccgg cggcggctgc ccggcctgat ccacctggat caccacggct ctgacgagcg
      361 acccgcctcc gggcgggccg ctcgtgtttt gctcccggtc gagcgtggac gcctccgccc
      421 ctcggcgggg tcgtggccgc tcgggcggat ggaagtggca ccggcttggc ggggcgggca
      481 acatgcgtcg cacatctgac gggtcgtcag atgtggccga cgaacgggag cctcgccatg
      541 accaccgagc cggaccgggc cgaagcgccg gtcctcgtgc acgacgaggc acgggtccgc
      601 accctcacgc tcaaccggcc cggcgcgctc aacgcctgca acgaagcgct ctacgacgcg
      661 ctcgccaacg ggctcctcga ggccgcagcg gacccgaatg tcgccgtcgt gctcctcacc
      721 ggcgccggcc gggcgttctg cgccggcacc gacctcgtcg agatggcggc tcgggtgatg
      781 gagcccgacg acttcgtcga gggcgagcac gggttcttgg ggctgatcga cgcgctcacc
      841 gacttcccca agccgctcgt catcgccgtc aacgggttgg gcctcggcat cggggcgacg
      901 atcctcggct tcgccgacct cgtcttcatg agcaccgccg ccaagctcaa gtgcccgttc
      961 accagcctgg ccgtggcccc cgaggcggca tcgagcctcc tcttcccgca gctggtcggc
     1021 cgccagaacg ccacgtggat gctcctcagc tccgagtgga tctccgccgc cgaggccaag
     1081 gagatgggcc tggtctggaa gctcaccgag cccgacgagc tgctgggagc cgctcgccag
     1141 cacgccgagc acctcgccgc cctcccgatc tccagcctgg tcgcctgcaa gcgggtcatc
     1201 accgagccgc tgcgcccgca gatcgaggcg gctcgccagc gcgagaacga cgccttcgtc
     1261 gagctcctgg gcagcccggc caacctcgag gccttcgccg ccttcgccga gggccgcaag
     1321 cccaacttcg tcgacctccc tggcggcggc tgaccccggt tcggtcgccc agacgggatg
     1381 cgaacgtcgt aggtcagatc gccccttccc gcacgctggc ctacgaagtt cgtgtgggtg
     1441 gggtcgtgac ccccggaacg tcgtcggcga gatcgaccat ctcggtgctc tgacctacga
     1501 cgttcggctc gatccggcgg gcggtgggag tggggtgggg gtgcatcggg caccatggcg
     1561 gcgatggacc aggccggcga tgcgctcgcg accgtcgcgc cggcgctggc gttcctcctc
     1621 gtcggcgtgc ccctcgctgc tctgctcgac gggctcgggt tcttcgactc ggtggccggg
     1681 ctcctcgccg agcggttcgg ccagatcccc gtcttggcgc tgtgggccct cgccgcggcc
     1741 accacggtgg cgctcaacct cgacacgacc gtcgtcttgc tcacgccgct gtacgtgcgg
     1801 ctggcccggc ggtcgggggt ggacccggtt ccgctcgcgc tgatcccgct gctcctggcg
     1861 gccttcgcgt cgtcggtgct gcccatctcc aacctgacca cgctcatcgc cgccgagcag
     1921 ctcgatctca gcgtcggcga cgtgctcggc cacctcgggc tgcccagcct ggccgcggcg
     1981 tccatcggct ggatcgccta ccgccgcatc cacccgaccc ggctggcgg
//
LOCUS       DATMFS010000102         3865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_4818, whole genome shotgun sequence.
ACCESSION   DATMFS010000102 DATMFS010000000
VERSION     DATMFS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4818"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..2564
                     /locus_tag="VNQ33_01905"
     CDS             84..2564
                     /locus_tag="VNQ33_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96880.1"
                     /translation="MNAVIRPAAVRCCVVLAVTALTIGSLLAPPATAAPARIAAPPRP
                     VGVAGAALPDPGVAGPEAVDELSYDFGDQAYRPTGFPAAVEVKAKVYAPQTIAGRAPL
                     VILQHGRHATCATATVTLLEWPCPASVPEIPSYQGYDALGRLLASHGMVVVSIGANGI
                     NANDGFLDDGGASARGQLILEHLRRWKGWDASATGAPFGSRFVGRIDLDRIGLMGHSR
                     GGEGIAAAVQLNQRSVNRFGIKAAMALAPVDFGRRVIGGVELGVILPYCDGDVYDLQG
                     ASYYDDGRYATPGDTSSKMTALLYGANHNFFNTVWTSGPGSFDDAGVFIEGGGQDTRA
                     AADPCRPGGSGRLTAAQQEQAGAALMAGFLRRTLGPEPNLQPLVTGTAPYPPSTSPAR
                     WSIAYHAGDRLDIESWASADTYRRTPTGQVTDIRGVNTGLVCNPSDEGGFGQPDGVRV
                     VTTRCAAATFTGLVNDTGALDAGWIRQGTSVRIPFAAAGIDVAGYDGVRMRVAVPNDT
                     RNAARAKQDFTVVLEDADGHRAAVPALSGTNGLQRLNAGTVQHAVLNGVRLPLSSFAG
                     IDVTRVRAIELRFDRTGAGRLAIADLAFTQEGLGSAAANTTGVGPITPPASPCRRTSA
                     ARWACAVGQLAWGRDPTTAEISWLGAGYATAAGRKRIVARALGGPTAHGLRHAQFLQT
                     YLQDEVDPAWVSSSLSANGKKHWDTATVDLVGAFAFSSASLATPDDVVRAAYESILGR
                     PADTGGLAHWAPKVRNGGARAMAANLVRTEAARGRIVDERYAQVLGRAADPAGRAYWV
                     KRLATPGGEQALMASLMATEAFRTWAIR"
     gene            2617..3465
                     /gene="truA"
                     /locus_tag="VNQ33_01910"
     CDS             2617..3465
                     /gene="truA"
                     /locus_tag="VNQ33_01910"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWJ96881.1"
                     /translation="MTLFDPVPAAEAPVAGPTERIRMVVAYDGAPFSGFARNEGVRTV
                     GGDLTQAIATVLGTPVELTCAGRTDKGVHARAQVVTFDAPVGLVDPARLTKSLNKLCG
                     PAIAVWGIQAVDAAFDARFSATARRYRYRIHNSPTPDPFTADSAWHVDTPLRLHTMVL
                     ACDAVIGEHDFAAFCRRPKRRDGLEASLVRRVTEAVWFDDGEGQLRFEIEASSFCHQM
                     VRSVVGTLVDVGRGRKRPGDVLGIIRSGDRDRAGDLAPPHGLTLWTVRYPGWSSDEGR
                     DTWSSA"
     gene            3568..>3865
                     /locus_tag="VNQ33_01915"
     CDS             3568..>3865
                     /locus_tag="VNQ33_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ96882.1"
                     /translation="MPIAFHWFLPTNGDGRRLVEPERSVPGTAGGQRPATFNYLTQIT
                     LAAEALGFEAALTPAGPWCDDAWVTTAALAQRTERLKFLVAFRPGVTSPTLAAQM"
BASE COUNT          510 a         1501 c         1350 g          504 t
ORIGIN      
        1 aggggtctca ccgccgggca accggtcagc taccagtggg agggcggacg acccatcgcc
       61 gccgggaagc tggaggcggc ccggtgaacg ccgtcatccg ccccgccgcc gttcgctgtt
      121 gcgtcgtcct cgccgtcacc gcgctcacca tcggcagcct gctggccccg ccggccacgg
      181 ccgcaccggc tcggatcgcg gctccgcccc gcccggtcgg ggtcgcgggc gccgcgctgc
      241 ccgatcccgg ggtcgccggc cccgaggccg tcgacgagct gagctacgac ttcggcgacc
      301 aggcctaccg accgacgggc ttcccggccg cggtcgaggt caaagccaag gtgtacgccc
      361 cgcagaccat cgccgggcgg gccccgctgg tgatcctcca gcacggccgc cacgccacct
      421 gcgccaccgc caccgtgacc ctgctcgagt ggccgtgccc cgcctcggtg cccgagatcc
      481 cgagctacca gggctacgac gccctcggcc ggctgctcgc gagccacggc atggtggtgg
      541 tctccatcgg cgccaacggc atcaacgcca acgacggctt cctcgacgac ggtggggcct
      601 ccgcccgcgg ccagctcatc ttggagcacc tccgacggtg gaagggctgg gatgcgagcg
      661 ccacgggggc gccgttcggc tcgcggttcg tgggccgcat cgacctcgac cggatcggtc
      721 tcatgggcca ctcgcgcggc ggcgagggga tcgcggcggc ggtccagctc aaccagcgga
      781 gcgtcaaccg cttcggcatc aaggcggcga tggccctcgc gccggtcgac ttcggccgcc
      841 gggtgatcgg cggcgtcgag ctcggcgtca tcctgccgta ctgcgacggc gacgtgtacg
      901 acctccaagg cgcgtcctac tacgacgacg ggcgctacgc gactccaggg gacacctcct
      961 cgaagatgac ggccctgctc tacggggcca accacaactt cttcaacacg gtgtggacct
     1021 cggggccggg ctcgttcgac gacgcgggcg tgttcatcga gggcggaggc caagacaccc
     1081 gggccgctgc cgacccgtgc cggcccggcg ggagcggccg tctcaccgct gctcagcagg
     1141 agcaggccgg cgccgcgctg atggccggct tcttgcgccg gacgctcggt cccgagccga
     1201 acctgcagcc gctcgtcacc ggcaccgctc cgtacccgcc ttcgacgagt cccgcccgct
     1261 ggtccatcgc ctaccacgcc ggagatcgcc tcgacatcga gagctgggcg agtgccgaca
     1321 cctaccggcg gacgccgacc ggccaggtca ccgacatccg gggggtcaac accggactcg
     1381 tctgcaaccc cagcgacgag gggggcttcg gccagcccga cggtgtccgc gtggtgacga
     1441 cccggtgcgc cgcggccacc ttcaccggcc tggtcaacga caccggagcg ctcgatgctg
     1501 ggtggatccg gcaggggacg tcggtgcgca tcccgttcgc cgcggcaggc atcgacgtgg
     1561 ccggctacga cggcgtccgg atgagggtgg ccgtccccaa cgacacccgc aacgcggcgc
     1621 gggccaagca ggacttcacc gtcgtgctcg aggacgccga cggccatcgg gcggcggtcc
     1681 ccgcgctcag cgggacgaac ggtctccagc gcctgaacgc cggcaccgtg cagcatgccg
     1741 tgctcaacgg cgtgcggctg ccgctctcgt cgttcgccgg catcgacgtg acccgggtgc
     1801 gggcgatcga gctgcgcttc gaccgcaccg gcgccggccg cctcgccatc gccgacctcg
     1861 cgttcaccca agaggggctc ggctcggctg ccgccaacac cacgggcgtg gggccgatca
     1921 ccccgccggc ctccccgtgc cgccggacca gcgccgcccg ctgggcctgc gccgtgggcc
     1981 agctggcgtg gggccgcgac cccaccacgg ccgagatctc gtggctgggc gccggctacg
     2041 ccacagccgc gggacgcaag cggatcgtcg cccgggcgct cggcgggccc accgcccacg
     2101 gcctccgcca cgcccagttc ctgcagacct acctccaaga tgaggtggac ccggcgtggg
     2161 tcagctcgtc gctgtccgcc aacggcaaga agcactggga cacggccacg gtcgacctgg
     2221 tcggtgcctt cgccttctcg agcgcatcgc tcgccacgcc cgacgacgtg gtgcgggcgg
     2281 cgtacgagtc gatcctgggc cggcccgccg acaccggcgg cctcgcgcac tgggcgccga
     2341 aggtgcgcaa cggcggggcc cgtgcgatgg ctgccaacct ggtccgcacc gaggcggcgc
     2401 gggggcggat cgtcgacgag cgctatgcgc aggtcctcgg gcgcgccgcc gaccccgctg
     2461 gtcgtgcgta ctgggtgaag aggctggcga cccccggtgg ggaacaggcg ctcatggcct
     2521 cgctcatggc gaccgaggcg ttccgcacct gggccatccg ctgaccacgg tgggcccggc
     2581 cccggccggc tgcggccgcg ttagcgtccg cagccgatga ccctcttcga cccggtgccc
     2641 gccgcagagg cccccgtcgc cggccccacc gagcggatcc gcatggtcgt cgcgtacgac
     2701 ggcgcgccgt tctcgggctt cgcccgcaac gagggcgtgc ggaccgtcgg cggcgacctc
     2761 acccaggcca tcgccaccgt cctcggcacc ccggtggagc tgacgtgcgc cggccgcacc
     2821 gacaagggcg tgcacgcccg ggcccaggtc gtcacgttcg acgcgccggt gggcctcgtc
     2881 gacccggccc gcctcaccaa gtcgctcaac aagctgtgcg ggccggccat cgccgtgtgg
     2941 ggcatccagg cggtcgatgc cgccttcgac gctcggttct ccgccaccgc ccgccgctac
     3001 cggtaccgca tccacaacag cccgacgccg gacccgttca ccgccgactc ggcctggcac
     3061 gtcgacaccc cgctgcggct gcacaccatg gtcctggcgt gcgacgcggt gatcggcgag
     3121 cacgacttcg cagcgttctg ccgtcggccc aagcgccgcg acggactgga ggcgagcctg
     3181 gtgcgccggg tgaccgaggc ggtgtggttc gacgacggcg aggggcagct gcgcttcgag
     3241 atcgaggcgt cgtcgttctg ccaccagatg gtccgctcgg tcgtcgggac gctcgtcgac
     3301 gtcgggcggg gacggaagcg gccgggtgac gtcctcggga tcatccgctc cggggaccgg
     3361 gaccgggcag gagatctggc gccgccccac ggcctgaccc tctggacggt ccgctacccg
     3421 ggctggtcct ccgacgaggg ccgagacacc tggtcgtccg cctgaccgag ggcggtccca
     3481 cgttgacgct gcgcgaacgc gacccccata ctccatcggt ctgaccagcc tccgcagacc
     3541 tcgccgcatc gccccagggt ggaccccatg ccgatcgcct tccactggtt cctgcccacg
     3601 aacggcgacg gccgtcgcct ggtggaaccg gaacgatccg tccccgggac cgccggtggc
     3661 cagcggccgg cgacgttcaa ctacctgacc cagatcaccc tggcggccga ggcgctcggg
     3721 ttcgaggcgg cgctcacccc ggcgggcccg tggtgcgacg acgcatgggt caccaccgcc
     3781 gccctcgccc agcggaccga gcggttgaag ttcctggtgg ccttccggcc cggcgtgacc
     3841 tcccccaccc tcgcggcgca gatgg
//
LOCUS       DATMFS010000103         4044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_50530, whole genome shotgun sequence.
ACCESSION   DATMFS010000103 DATMFS010000000
VERSION     DATMFS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50530"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VNQ33_01920"
     CDS             complement(<1..770)
                     /locus_tag="VNQ33_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96883.1"
                     /translation="MTLTMPNRVLDATPIETLDEWVAAGGARGLAKAWKAGPDEVIEI
                     VTDSGLRGRGGAGFPTGAKWATIRADRSADLPATVVVNGAEGEPGSYKDRSILLANPY
                     RVIEGALIAAAVVEADEVVVALKAGSPEVVDRVRRAIDEIRDAGWLVGLDVDVVVGPE
                     HYLYGEETALLEVVSGREPFPRIAPPWRRGAEDIGDDSALAGRAELAAPGASTPPALV
                     NNVETMGNVPGIVSNGAAWFRAVGTEASPGTMVVTVSGG"
     gene            complement(804..1964)
                     /locus_tag="VNQ33_01925"
     CDS             complement(804..1964)
                     /locus_tag="VNQ33_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWJ96884.1"
                     /translation="MPTDVVIVSAARTPIATAYKGSLTGVDAFALAETAIGAAVKRSG
                     IPADQIEDMGFGESMQGGGNIGRNVAVRLGLTGVPGVATQRWCASGMSATQWVAANIA
                     AGMIDVGIGGGTESMSTAPGTSKPGPDGAPAFWLSPGNPDTPEAPPFNMALTVGDNTS
                     RLAGVSREEADEWAFTSHRNAVRAIDEGRFENEIVPVDLPGGGRFTTDEHPRRNSTLE
                     KMASLAVLNPDVEGATTTAANSSGLNDAAAALVLCSREYADSHGLKPLAAIRGWASVG
                     LDPVETGLAPQKALPKALAKSGISIADLQSVEVNEAFASVAVAFTRGLGLDPAIVNVN
                     GSGCSLGHPIACTGARMIVTMVNELIRADQQWGAVAMCAAGGMGSATVIERL"
     gene            2060..2782
                     /locus_tag="VNQ33_01930"
     CDS             2060..2782
                     /locus_tag="VNQ33_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWJ96885.1"
                     /translation="MLIAGLSDFLVGDLTDWVVDVIKAIGYLGVALLVAVESIFPPIP
                     SEVVLPAAGLASSRGEANLVLMVAAATVGSVIGAWALYLLSAAIGDVRLHALVARYGR
                     WIGVKPKDLERAEAWFDDHSSTAVLICRCIPLIRSLVSIPAGFRRMNPVAFTIYTAIG
                     SLVWNAVLIGAGYELGDRWEDVADWVGILQYVVIAAIGVGLGRWIWTRFISPTHRARL
                     AAEHEEHQRQAAAAQAVLDATD"
     gene            complement(2873..3718)
                     /locus_tag="VNQ33_01935"
     CDS             complement(2873..3718)
                     /locus_tag="VNQ33_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWJ96886.1"
                     /translation="MTWRLQVTHTTKYRYSAPVAQSYNEARLEPRSDRHQAVLASRVE
                     VEPSTRVVRHVDYWGSVVHHFDLQVPHQALTVIGRSTVETATEPARAGDATWDDLADP
                     DIRDQFEELLVPTETVTWDEELAAVAADLAAVHDTPAKAAAAAVGWVNDRLEYQKGST
                     GVRTTSPEVLAAGSGVCQDFAHVSLALLRAMGIPAWYVSGYLHPDPEAEIDQVIVGES
                     HAWVAAFTGTVWPLDPTSLNPVEERHIRVAAGRDYHDVAPFRGIYAGRADQALEVSVE
                     LVRKA"
BASE COUNT          538 a         1473 c         1437 g          596 t
ORIGIN      
        1 ccacccgaca cggtgacgac catcgtcccc ggcgacgcct cggtcccgac ggcgcggaac
       61 cacgccgcac cgttgctcac gatgccgggc acgttgccca tggtctcgac gttgttcacg
      121 agcgcgggtg gcgtcgaggc gccgggggca gcgagctcgg cccgccccgc gagcgccgag
      181 tcgtcgccga tgtcctcggc gcctcggcgc cacggcgggg cgatgcgcgg gaacggctcg
      241 cggccgctga cgacctcgag cagggcggtc tcctcaccgt agaggtagtg ctcgggcccg
      301 accacgacgt cgacgtcgag cccgacgagc caaccggcgt cacggatctc gtcgatggcc
      361 cgccggaccc ggtccaccac ctccggcgac ccggccttca gggccaccac cacctcgtcg
      421 gcctccacca cggcggcggc gatcagcgcg ccctcgatca cccggtacgg gttggcgagg
      481 aggatcgacc gatccttgta ggacccgggc tcgccctcgg cgccgttgac cacgacggtc
      541 gccggcaggt cggcggatcg atcggcccgg atcgtcgccc acttggcgcc ggtggggaac
      601 ccggcgccgc cgcggcctcg aagaccgctg tcggtgacga tctcgatgac ctcgtcgggg
      661 ccggccttcc acgccttggc cagcccccgg gcgccgcctg ccgccaccca ctcgtcgagg
      721 gtctcgatcg gggtcgcatc gaggacccgg ttgggcatgg tgagcgtcat gggcctccgg
      781 tacccggccg gaggcccgcg cagtcagagg cgctcgatga cggtggcgga ccccatgccg
      841 ccggccgcgc acatggcgac cgcgccccac tgctggtcgg cgcggatcag ctcgttgacc
      901 atcgtgacga tcatccgggc accggtgcag gcgatggggt ggccgagcga gcagcccgag
      961 ccgttgacgt tgacgatcgc cgggtcgagc cccaggccgc gggtgaaggc gacggccacc
     1021 gaggcgaacg cctcgttgac ctcgaccgac tgcaggtcgg cgatggagat acccgacttg
     1081 gccagcgcct tgggcagggc cttctgcggg gccagaccgg tctcgaccgg atcgaggccg
     1141 accgacgccc agccgcggat ggcggcgagg ggcttgaggc cgtgggagtc ggcgtactcg
     1201 cggctgcaca gcaccagcgc ggcggcggcg tcgttcagcc ccgaggagtt ggcggcggtg
     1261 gtggtggcgc cctcgacgtc ggggttcagc accgcgagcg acgccatctt ctcgagcgtc
     1321 gagttccggc gcgggtgctc gtcggtcgtg aaccggcctc caccgggcag gtccacgggc
     1381 acgatctcgt tctcgaagcg gccctcgtcg atggcccgca cggcgttgcg gtgcgacgtg
     1441 aaggcccact cgtcggcctc ctcgcgggac acgccggcga ggcgggaggt gttgtcgccc
     1501 acggtgagcg ccatgttgaa cggcggggcc tcgggcgtgt cggggttgcc gggcgacagc
     1561 cagaacgccg gagccccgtc ggggccgggc ttcgaggtgc ccggcgcggt ggacatggac
     1621 tcggtgccgc caccgatgcc gacgtcgatc atgccggcgg cgatgttggc ggcgacccac
     1681 tgcgtggccg acatgcccga ggcgcaccag cgctgggtgg cgacgccggg gacgccggtg
     1741 agcccgaggc gcacggccac gttgcgcccg atgttgccgc cgccctgcat cgactcgccg
     1801 aagcccatgt cctcgatctg gtccgccggg atgcccgagc gcttgacggc ggcgccgatg
     1861 gcggtctcgg ccagagcgaa ggcgtcgacg cccgtgagcg aacccttgta ggcggtggcg
     1921 atcggggtgc gggcggcgct gacgatgacg acatcggtgg gcatggcaga gctcctggga
     1981 cagatcggaa cgtcgaggac gccgaattct gacacaccgt cagatttgag agcgattccc
     2041 gatttcctac gatcacccga tgctgatcgc tggactgtcc gacttcctcg tgggcgacct
     2101 caccgactgg gtcgtggatg tgatcaaggc catcggctac ctgggcgtcg ccctcctggt
     2161 ggcggtggag agcatcttcc cgcccatccc ctccgaggtg gtgctgcccg ccgccggcct
     2221 ggcctcgtct cggggcgagg ccaacctcgt gctgatggtc gctgccgcca ccgtcggctc
     2281 ggtcatcggc gcctgggccc tctacctcct gtcggcggcg atcggcgacg tccggctcca
     2341 cgccctcgtc gcccgctacg gccggtggat cggcgtgaag ccgaaggacc tggaacgggc
     2401 cgaggcctgg ttcgacgacc actcctccac cgccgtgctg atctgccggt gcatccccct
     2461 gatccgctcg ctggtgtcga tccccgccgg gttccgccgc atgaaccccg tggccttcac
     2521 gatctacacg gccatcggca gcctggtctg gaacgccgtg ctcatcggcg ccggctacga
     2581 gctcggcgac cgctgggaag acgtcgccga ctgggtcggc atcctccagt acgtcgtcat
     2641 cgccgccatc ggcgtcggcc tcggccgctg gatctggacc cgcttcatct cgcccacgca
     2701 ccgggcccgc ctcgccgcgg agcacgagga gcaccagcgc caggccgccg ccgcgcaggc
     2761 ggtcctcgac gccaccgact gactccctcg agcaccgacg ccgctgcggg gtggagagcc
     2821 aggaggcctc ctgaccgcga ggacaggtgg cgccagcgca ccgccagccc gctcaggcct
     2881 tgcggaccag ctccacgctg acctcgagcg cctggtcggc ccgaccggcg tagatgcccc
     2941 ggaacggggc cacgtcgtgg tagtcccgcc cggcggccac ccggatgtgg cgctcctcca
     3001 ccgggttcag gctggtgggg tcgagcggcc agaccgtgcc cgtgaacgcg gcgacccagg
     3061 cgtggctctc tccgacgatc acctggtcga tctccgcctc cggatccgga tggaggtagc
     3121 ccgacacgta ccaggccggg atgcccatcg cccggagcag ggcgagcgac acgtgggcga
     3181 agtcctggca gacaccggag cccgccgcca gcacctcggg cgaggtggtg cgcacgccgg
     3241 tgctcccctt ctggtactcg agccggtcgt tgacccatcc gaccgcggcc gcagccgcct
     3301 tcgccggggt gtcgtggacg gcagcgaggt cggcggccac tgccgccagc tcctcgtccc
     3361 aggtcacggt ctcggtcggg acgagcagct cctcgaactg gtcgcggatg tccgggtcgg
     3421 ccaggtcgtc ccacgtcgca tcaccggccc gagcgggctc ggtggccgtc tcgaccgtcg
     3481 agcggccgat gaccgtcagc gcctggtgcg gcacctgcag atcgaagtgg tggaccaccg
     3541 acccccagta gtcgacgtgg cgcacgaccc gcgtggacgg ctccacctcc acccggctgg
     3601 ccagcaccgc ctggtgccgg tcgctccgcg gctcgagccg ggcctcgttg tacgactggg
     3661 ccaccggcgc ggagtaccgg tacttcgtcg tgtgcgtcac ctgcagccgc cacgtcatcg
     3721 gatcgcaccc atcctcatgc cggccggtcc acgttccacc tcgtcgccgt accgccgcac
     3781 gagatgaccc acgcaccgct cgcggttcgg ctgtctgcac ccgtgctcgt tgcgagcgga
     3841 acgtggaaca ggtccgtcat cgtgttcgcg tccatcctca tgccggccgg tccacgttcc
     3901 actcgatgaa cgcggcgcgt tggaagtagc gctgggtcac gagctcgctg gcgtaggcac
     3961 aggcggcctg gaggcgcatg acggtgccgt tgaggtcggc ggccagctcg gcgggatcgg
     4021 tgtaggcgag ctcgctgcgg atgc
//
LOCUS       DATMFS010000104         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_143839, whole genome shotgun sequence.
ACCESSION   DATMFS010000104 DATMFS010000000
VERSION     DATMFS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143839"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="murD"
                     /locus_tag="VNQ33_01940"
     CDS             complement(<1..1260)
                     /gene="murD"
                     /locus_tag="VNQ33_01940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWJ96887.1"
                     /translation="MTEPLASEALVPGRVLVVGLGVMGRAVVRALVKRNVEVTVTDDG
                     LGADGPARAVELGAAWVDPPADWEALIAHHGAVVPSPGIPERHPLFASALATGVPIRS
                     ELDLAAAWDQRPIATITGTNGKTTVTTLVTEMLVASGLRAVPAGNVDVPLVEAIEDPD
                     TEVFVVEASSFRLTFATAHQPVVGAWLNFAPDHLDVHRDLTGYEAAKARIWANHGQGG
                     LAVANADDPVVMRWAPEGERTQTFGIHEPADWHIADGVLRGPDGLDLLPVGELTRSLP
                     HDRSNALAAAATALAAGATPDGIRAALRAFRSLPHRVELVGEAGGVRWYDDSKATAPH
                     ATLAAVAGFESAVLIAGGRNKGLDLTGLGDLAPHLRAVVAIGEAAADVEAAFAGRTPT
                     VTASSMEEAVALAAGLAQPGDDVVLSPA"
     gene            complement(1257..2321)
                     /gene="mraY"
                     /locus_tag="VNQ33_01945"
     CDS             complement(1257..2321)
                     /gene="mraY"
                     /locus_tag="VNQ33_01945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWJ96888.1"
                     /translation="MIALLMAGGLSLAASLVGTRYLIVWLTNHQIGQNIREEGPEGHK
                     VKAGTPTMGGIAIVAGAVIGYSVAHLRSRVVFTRSGMFVLALVVGAGLVGFLDDWIGI
                     TRERNLGLNKRTKVLGLLFVAGSFGVATVLWTGVHTTLSFTRYDSFDSSALLNIDFGK
                     VGWVLFSVLVIMAVTNGVNLTDGLDGLAAGSSIFAFASFTIIGFWAFRNPLVYDVEHA
                     LDLAVVAAAMLGGCAGFLWWNAHPAKIFMGDTGSLAIGAALAGLALTLNTMLLLPIIG
                     ALFVVETLSVIIQVFSFRVFGRRVFRMAPIHHHYELRGWPETTVIVRFWMIAGLATAL
                     ALGMFYADFTTLPPIPAFAS"
     gene            complement(2318..>2533)
                     /locus_tag="VNQ33_01950"
     CDS             complement(2318..>2533)
                     /locus_tag="VNQ33_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96889.1"
                     /translation="IEAPTDLRIEPDRAAAIALAVTEAEPGDVVLVAGKGHETTQTIG
                     EAVVPFDDRQVLRGALAAAGWSGGAAS"
BASE COUNT          383 a          864 c          975 g          311 t
ORIGIN      
        1 ggccggcgag aggacgacgt cgtcgcccgg ctgcgcgagg ccggcggcca gggccacggc
       61 ctcctccatc gacgacgcgg tgacggtggg cgtgcggccg gcgaaggccg cttcgacgtc
      121 ggcggcggcc tccccgatgg cgacgacggc gcgcaggtgc ggggcgaggt cgccgagccc
      181 ggtcaggtcg aggcccttgt tgcgcccgcc ggcgatgagc acggccgact cgaagccggc
      241 caccgcggcc agggtcgcgt gcggcgccgt ggccttcgag tcgtcgtacc agcgcacccc
      301 accagcctcg cccaccagct ccacgcgatg ggggaggctg cgaaaggcac ggagagccgc
      361 tcggatgccg tcgggcgtgg cgcccgcggc cagggcggtg gccgcggcgg ccagcgcgtt
      421 cgaacggtcg tggggcagcg accgggtcag ctcgccgacc ggcagaagat ccagcccgtc
      481 gggcccgcgc agcaccccgt cggcgatgtg ccagtcggcc ggctcgtgga tgccgaaggt
      541 ctgggtgcgc tccccctccg gtgcccagcg catgacgacg gggtcgtcgg cgttggcgac
      601 ggcgaggccg ccctgaccgt ggttggccca gatccgcgcc ttggccgcct cgtagccggt
      661 gaggtcgcgg tgcacgtcga ggtggtcggg ggcgaagttc agccacgcgc ccaccaccgg
      721 ctggtgggcc gtggcgaacg tgagccggaa cgacgacgcc tcgaccacga agacctcggt
      781 gtcggggtcc tcgatggcct cgacgagcgg gacgtcgacg ttgccggcgg gcacggcccg
      841 cagcccggag gccaccagca tctcggtcac gagggtggtg accgtggtct tgccgttggt
      901 gccggtgatg gtggcgatcg gccgctggtc ccaggccgcg gccaagtcga gctcgctgcg
      961 gatcggcacg ccggtggcga gcgccgaggc gaagagcgga tggcgctcgg ggatgccggg
     1021 gctgggcacc acggcgccgt ggtgcgcgat cagcgcctcc cagtctgcag gcggatcgac
     1081 ccatgcggcg cccagctcga cggcgcgggc cggaccgtcg gcgcccaggc cgtcgtcggt
     1141 cacggtgacc tcgacgttcc gcttcacgag cgcgcgcacg accgcacgtc ccatcacccc
     1201 gaggccgacg accagcaccc ggccggggac gagcgcctcc gaggcgagcg gctcggtcac
     1261 gatgcgaacg ccgggatggg gggcagggtg gtgaagtcgg cgtagaacat cccgagcgcg
     1321 agcgcggtgg cgagcccggc gatcatccag aagcggacga tgaccgtggt ctcgggccac
     1381 ccgcgcagct cgtagtggtg gtggatcggc gccatgcgga agacgcggcg tccgaacacc
     1441 cggaacgaga acacctggat gatgaccgac agcgtctcga ccacgaacag ggccccgatg
     1501 atgggcagca gcagcatcgt gttgagcgtg agggccaagc cggcgagggc ggcgccgatg
     1561 gccagcgacc ccgtgtcgcc catgaagatc ttcgccgggt gcgcgttcca ccacaggaag
     1621 ccggcgcacc cgccgagcat cgcggcagcc acgaccgcca ggtcgagcgc gtgctcgacg
     1681 tcgtagacga gcgggttccg gaacgcccag aagccgatga tcgtgaagct ggcgaaggcg
     1741 aagatcgacg acccggccgc cagcccgtcg agcccgtcgg tgaggttcac gccgttggtg
     1801 accgccatga tcacgaggac cgagaacagg acccagccga ccttgccgaa gtcgatgttc
     1861 aacagggccg aggagtcgaa cgagtcgtac cgggtgaacg acagcgtcgt gtgcacgccg
     1921 gtccacagca cggtggcgac gccgaagctg ccggccacga acagcaggcc gaggaccttc
     1981 gtgcgcttgt tgaggccgag gttgcgctcg cgggtgatgc cgatccagtc gtcgaggaag
     2041 ccgaccagcc cggcaccgac cacgagcgcg agcacgaaca tgcccgagcg ggtgaagacc
     2101 acgcggctgc gcaggtgggc caccgagtag ccgatgaccg cgccggcgac gatggcgatg
     2161 ccgcccatgg tgggggtgcc ggccttgacc ttgtggcctt cgggcccctc ctcgcggatg
     2221 ttctggccga tctggtggtt cgtgagccag acgatgaggt agcgggtgcc gacgagcgac
     2281 gcggcgaggc tgaggccacc ggccatgagg agggcgatca cgaggcggcc cccccgctcc
     2341 agccggcggc ggccagcgcg ccgcgcagga cctggcggtc gtcgaacggc acgacggcct
     2401 cgccgatcgt ctgggtggtc tcgtgcccct tgcccgcgac gaggacgacg tcgccgggct
     2461 ccgcctcggt caccgccaac gcgatggcgg cggcccggtc gggctcgatc cggaggtcgg
     2521 tgggggcttc gat
//
LOCUS       DATMFS010000105         6123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_166567, whole genome shotgun sequence.
ACCESSION   DATMFS010000105 DATMFS010000000
VERSION     DATMFS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166567"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1131
                     /locus_tag="VNQ33_01955"
     CDS             256..1131
                     /locus_tag="VNQ33_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWJ96890.1"
                     /translation="MAEGTDPRWTAAVEVLDRINASATPPTLVREGLSLLVRQDGVLV
                     RSRLRSDEPVAAREVEVARALADAAVASVPLVAGKGQPWIVGGCVVTAWRWEEVTGVP
                     SPADLGALARALRERTVGSQVFDVPRFDPLSAIRGAVARVPIGDPQGDFVRKRAQELS
                     ADWARIADRDPAGTAVVHGDLHQDNVLCTTEGALLTDLELAGAGPSSYDTAPAVVMVE
                     RYGADPATLAAFVDAQGLDPRGWVGFATCLAVYELWVTAWSVGVRDRSPELRAEAAVR
                     VGCLRDQTCEPWRLL"
     gene            complement(1290..2885)
                     /locus_tag="VNQ33_01960"
     CDS             complement(1290..2885)
                     /locus_tag="VNQ33_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWJ96891.1"
                     /translation="MTAPDLAAAATVIDLASNVVDTGARHLASLEGGPDANQVLAYDL
                     AHAASAVATAKGLLDYGAKGEIEGKITCAFVADATAELAGKLFGREAGWGVEPGALDG
                     AREFITAYKAPEFLASITDPGPRHLDSDFELVQDTFRRYAEQELAPRAEHIHRTNGDI
                     PEDIIEGLAEMGAFGLSIPSEYGGYGEGGEGEYIGMVVTTEELSRGSLGAGGSLITRP
                     EILARALMAGGTEEQKHEWLPKLASAEVMNAVAVTEPDYGSDVAGVKVTATPTTGGWL
                     INGVKTWCTFGARADVLLVLARTDTDRSIGHRGLSMFIVPKERGDSHGFELTQPAGGG
                     ADGTGKMEGRPIDTIGYRGMHSYEVAFDDWFVPAENLIGLDEGQGRGFYYQMAGFENG
                     RLQTAARAIGVMQAAYEKARTYAQDRVVFGRPIGDYQLTQAKLGRMAVVIQSSRQFAY
                     VVAKLMAKGEGQAEASMVKAYVCKAAEWVTREAMQIHGGMGYAEEYDVSRYFVDARVL
                     SIFEGADETLCLKVIAKKLVASA"
     gene            complement(2949..5531)
                     /locus_tag="VNQ33_01965"
     CDS             complement(2949..5531)
                     /locus_tag="VNQ33_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ96892.1"
                     /translation="MRDSFREFQRPLVDIDEDGFLHPLFLQRYLAIAALALGSALFGT
                     FPASLPLLIIVVGYAANAVAHVQAKRTGAAPIWMHFTDMAGVLIFPAISPDIAAPATL
                     VMLAVVSLAASVSGLGPALATTLLGTLGLLAINAWDPLPDAALLIAGFAIAALMIATA
                     VGQLAAVEDRVRRRLDTVVDNLDAILWVRNPGDDRFTFVNQRATTMLGWSEEEWLAPG
                     FWRANLHPSDLPATTETVARAVALGIDHEVSYRFRAADGHWVHLHDRVTVSVDGAGAA
                     KTLQGMSIDISDRVQIEHRVNQYADIVDRIDQALIVLRLEGDDDAGGEVLRLRAANPA
                     AERLMRRDLYSLIGDRVEDAFPALVGSRLRDRLVGVVERGVPLRVDDLLVQPVDADQR
                     VVTLRAFPLPDRSVAISLQDITDAVAASEALRRQALYDGLTGLPNRRLFDQELHRAVR
                     DAPTHGQQVALLVMDLDQFKEVNDALGHHVGDQLLRGIGDRLTREFDNALVARLGGDE
                     FALVLVGDVTEELARQVAGRIRDTLTEPFLMDDVRLQSNASIGIALFPDHADDVPTLI
                     QRADVAMYMAKRAGTGAAVYAAEHDRSSVERLTLIGDLPDAVSLDQFELHFQPCVDLR
                     TGIPVRAEALVRWNHPRLGRIAPDQFIELAELSGAIQPLTRWVLREGMIAATRWRAAG
                     HQIGLAVNLSVRNLYDPELVPYVAQELSISQVPAGDLVLELTETELMDDPGLAREIFT
                     QLGDLGVGTAIDDFGTGYSSLTYLRDLPLQEVKIDKSFVSEMHRRSEEFTIVRSMIDL
                     GHNLGLKVVAEGVEHADDLVLLRRLGCDLAQGFHLAEPLPLDELLTWLGQYSASMVLP
                     SAAS"
     gene            5668..>6123
                     /locus_tag="VNQ33_01970"
     CDS             5668..>6123
                     /locus_tag="VNQ33_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ96893.1"
                     /translation="MGTADGISDVAAEGFGAGAAAYEKGRPSYSDDVVAWLVERLGIA
                     PGRTVVDLAAGTGKLTRLLAATGASVTAIEPVDAMRDQLLQACPGVEAVSGTAEAIPL
                     PDRTVDALTVAQAFHWFDPAAALAEIARVLRPGGALGIVFNERDTREPWV"
BASE COUNT          821 a         2082 c         2361 g          859 t
ORIGIN      
        1 agctgatcga gctggcggcg caagccagcg aggacccggg cctgtggcgc ctccggtcgc
       61 gccgctacct cgctcgtgcc gaacaggaga ccagcctgat ggccaagggc gtcttcacgg
      121 acgcagcccg gtcgggcgac gaacggctgg ctgcgctcga gccctgagcg cccttcggcc
      181 gcctgccgcc gcgccctccg aggggccgga gcgaggcggt ccgccgcggc cgggggcggc
      241 agtagcgtgc cccccatggc tgagggcacc gatccgcgct ggacggcagc ggtcgaggtg
      301 ctcgaccgga tcaacgcctc ggccacgccg ccgacgctcg tgcgcgaggg gctctcgctg
      361 ctcgtccgcc aagacggcgt cttggtccgg tcccggctcc gctccgatga gcccgtcgcc
      421 gcgcgcgagg tggaggtcgc ccgcgccctc gcggacgccg cggtcgcgtc ggtgccgctg
      481 gtggccggga agggccagcc gtggatcgtc ggcgggtgcg tcgtgacggc gtggcggtgg
      541 gaggaggtga ccggcgtgcc gtcgccggcc gacctgggcg ctctggcccg ggcgctgcgg
      601 gagcggacgg tcggctccca ggtgttcgac gtgccccggt tcgatccgct gtcggcgatc
      661 cgcggcgccg tcgcccgtgt gccgatcggc gacccccagg gcgacttcgt gcgcaagcgg
      721 gcgcaggagc tgtcggccga ttgggcccgg atcgcggacc gcgacccggc cgggacggcc
      781 gtggtccacg gcgatctgca ccaggacaac gtgctctgca ccaccgaggg cgccctgctg
      841 accgacctcg agctggccgg tgccggaccc agctcctacg acaccgcgcc ggcggtggtg
      901 atggtcgagc gctacggcgc cgatccggcg acgctcgccg cgttcgtgga cgcccagggc
      961 ctggaccccc gagggtgggt gggcttcgcc acgtgcctgg ccgtgtacga gctgtgggtc
     1021 accgcctggt ccgtgggcgt ccgggaccgc tcgcccgagc tccgggccga agcggccgtg
     1081 cgcgtcggct gcctccgcga ccagacgtgc gagccctggc gcctgctctg agctgacgga
     1141 tcgctcgggc gcggcggatc tcatcgccca gctccgcccg ccgatcctca cggtccgctc
     1201 cggcggtcac ggccggtgcc cctcctgatc cggtccccgt ccccgacctt cggcgccgtc
     1261 cgggagggca gccagggagc cggtcccggt caggcggagg cgacgagctt cttggcgatg
     1321 accttgaggc agagggtctc gtcggcgcct tcgaagatgc tcagcacgcg agcgtcgacg
     1381 aagtagcggc tgacgtcgta ctcctcggcg tagcccatgc cgccgtggat ctgcatggcc
     1441 tcgcgggtga cccactcggc cgccttgcag acgtaggcct tgaccatgga ggcctcggcc
     1501 tggccctcgc ccttggccat gagcttggcc acgacgtagg cgaactggcg cgacgactgg
     1561 atgacgacgg ccatccggcc cagcttggcc tgggtcagct ggtagtcgcc gatggggcgg
     1621 ccgaacacga cgcggtcttg ggcgtaggtg cgggccttct cgtaggcggc ctgcatgacg
     1681 ccgatggccc gggctgcggt ctgcagccgg ccgttctcga agccggccat ctggtagtag
     1741 aagccgcggc cctgcccctc gtcgaggccg atcaggttct cggcgggcac gaaccagtcg
     1801 tcgaacgcga cctcgtagga gtgcatgccg cggtagccga tcgtgtcgat cgggcggccc
     1861 tccatcttcc cggtgccgtc ggcgccgccg ccggccggct gggtgagctc gaagccgtgg
     1921 ctgtcgccgc gctccttggg cacgatgaac atcgagaggc cgcggtggcc gatgctgcgg
     1981 tcggtgtcgg tgcgggcgag cacgaggagc acgtcggccc gggcgccgaa ggtgcaccag
     2041 gtcttgacgc cgttgatgag ccagccgccc gtggtggggg tggccgtgac cttcacgccg
     2101 gcgacgtcgg agccgtagtc gggctcggtc acggcgacgg cgttcatgac ctcggcggag
     2161 gccagcttgg gcagccactc gtgcttctgc tcctcggtgc cgccggccat gagggcgcgg
     2221 gcgaggatct cggggcgggt gatgagcgag ccgccggcgc cgagtgagcc gcgcgacagc
     2281 tcctcggtgg tgacgaccat gccgatgtac tcgccttcgc cgccttcgcc gtagccgccg
     2341 tactcggacg ggatggagag gccgaaggcg cccatctcgg ccaggccctc gatgatgtcc
     2401 tcggggatgt cgccgttggt ccggtggatg tgctcggcgc gcggcgccag ctcctgctcg
     2461 gcgtagcgcc ggaaggtgtc ctgcaccagc tcgaagtcgc tgtcgaggtg acggggcccg
     2521 gggtcggtga tcgaggccag gaactcgggg gccttgtagg cggtgatgaa ctcgcgggcg
     2581 ccgtcgaggg caccgggctc gacgccccag ccggcctcgc ggccgaagag cttgccggcc
     2641 agctcggcgg tggcgtcggc gacgaaggcg caggtgatct tgccctcgat ctcccccttg
     2701 gcgccgtagt cgaggaggcc cttggcggtg gcgacggccg acgcggcgtg ggccaggtcg
     2761 taggcgagca cctggttggc gtcgggtccg ccttcgagcg aggcgaggtg gcgggccccg
     2821 gtgtccacga cgttcgaggc caggtcgatc acggtggcgg cggcggcaag atccggagcg
     2881 gtcatgggcc gggatcgtac tttcgggtcc cggcccggct cacattggac gccggtcctg
     2941 acggtccgtc agctggccgc gctggggagc accatcgacg cggagtactg gccgagccag
     3001 gtcaggagct cgtcgagggg gaggggctcg gccaggtgga agccctgggc caggtcgcaa
     3061 cccaagcggc gcagcaagac gaggtcgtcg gcgtgctcga caccttcggc caccaccttc
     3121 aggccgaggt tgtggccgag gtcgatcatc gaccgcacga tcgtgaactc ctcggaccgg
     3181 cggtgcatct cgctgacgaa cgacttgtcg atcttcacct cctgcagcgg caggtcgcgg
     3241 aggtaggtga gcgacgagta cccggtgccg aagtcgtcga tcgccgtccc gacgcccagg
     3301 tcgcccagct gggtgaagat ctcccgcgcc agcccggggt cgtccatcag ctccgtctcg
     3361 gtgagctcga gcaccaggtc tccggcgggg acctgggaga tcgagagctc ctgggccacg
     3421 taggggacca gctccgggtc gtacaggttg cggacggaga ggttgacggc caggccgatc
     3481 tggtggccgg cggcgcgcca gcgggtggcg gcgatcatcc cctcgcgcag cacccaccgc
     3541 gtcaggggct ggatggcgcc cgacagctcg gccagttcga tgaactggtc cggggcgatg
     3601 cggccgaggc gggggtggtt ccagcgcacg agggcctcag cgcggacggg gatgccggtg
     3661 cgcaggtcga cgcagggctg gaagtgcagc tcgaactgat cgagcgagac ggcgtcgggc
     3721 aggtcgccga tgagcgtgag ccgctcgacg ctggagcggt cgtgctcggc ggcgtacacg
     3781 gccgcccccg tgccggcgcg cttggccatg tacatggcga cgtcggcccg ttggatcagg
     3841 gtcgggacgt cgtcggcgtg gtcggggaac aaggcgatgc cgatggaggc gttcgactgg
     3901 agccgcacgt cgtccatgag gaacggctcg gtgagcgtgt cgcggatccg accggccacc
     3961 tggcgggcca gctcctcggt gacgtcgccc accagcacca gggcgaactc gtcgccgccg
     4021 aggcgggcca cgagggcgtt gtcgaactcc cgggtcaggc ggtcgccgat gccccgcagc
     4081 agctggtcgc cgacgtggtg cccgagggcg tcgttgacct ccttgaactg atcgaggtcc
     4141 atgacgagga gggcgacctg ctggccgtgc gtcggcgcgt cgcgcaccgc ccggtgcagc
     4201 tcctggtcga agagccgccg gttcggcagg ccggtgaggc cgtcgtagag ggcctggcgg
     4261 cggagcgcct cggaggcggc caccgcgtcg gtgatgtcct gcagcgagat ggcgacggag
     4321 cggtcgggga gcgggaaggc gcgaagcgtc acgacccgct ggtcggcgtc gaccggctgg
     4381 acgaggaggt cgtcgacgcg cagcggcacg ccccgttcca cgacgcccac caggcggtcc
     4441 cgaaggcggg agccgacgag ggcggggaac gcgtcctcga cccggtcgcc gatgagcgag
     4501 tacaggtcgc ggcgcatcag ccgctcggcg gctgggttgg cggcccgcag gcgcagcacc
     4561 tcgcccccgg cgtcgtcgtc gccctcgagc cgcagcacga tcagcgcctg gtcgatgcgg
     4621 tcgacgatgt cggcgtactg gttgacccgg tgctcgatct gcacccggtc cgagatgtcg
     4681 atgctcatgc cctggagggt cttggcggcg ccggcgccgt cgaccgacac cgtgacgcgg
     4741 tcgtgcaggt ggacccagtg gccgtcggcg gcgcggaacc ggtagctgac ctcgtggtcg
     4801 atgccgaggg cgacagcgcg ggcgacggtc tcggtggtgg ccgggaggtc ggacgggtgc
     4861 aggttggcgc gccagaagcc cggcgccagc cactcctcct cgctccaccc gagcatggtg
     4921 gtggcgcgct ggttgacgaa cgtgaagcgg tcgtcgcccg ggttccgcac ccacaagatg
     4981 gcgtcgaggt tgtcgaccac ggtgtcgagg cgccgtcgca cccggtcctc gacggccgcc
     5041 agctggccca cggcggtggc gatcatcaag gcggcgatgg cgaacccggc gatcagtagc
     5101 gccgcgtcag ggagcggatc ccaggcgttg atcgccagca ggccgagcgt gcccagcagc
     5161 gtggtggcga gggccgggcc caggcccgac accgaagcgg cgagcgagac gacagcgagc
     5221 atcacgaggg tggccggcgc cgcgatgtcc ggcgagatcg ccgggaagat cagcaccccg
     5281 gccatgtcgg tgaagtgcat ccagatgggg gcggcgccgg tgcgcttggc ctggacgtgg
     5341 gccaccgcgt tggcggcgta cccgaccacg atgatgagca acggcaagga ggcggggaag
     5401 gtcccgaaca gggccgaacc cagcgccagg gccgcgatgg cgaggtagcg ctggaggaac
     5461 agcgggtgca agaacccgtc ctcgtcgatg tccaccagcg ggcgctggaa ctcccgaaac
     5521 gagtcgcgca agcgggatcg aactccggtc gcggcactca tcgtggccat catctcacct
     5581 ggcggggtca tgtcccacct ctcggaccca gcctgagggt gggagggcct cggcgcctac
     5641 catcagccgc ggcgaaggga gcaccagatg gggacggccg acggcatcag cgacgtggca
     5701 gcggagggct tcggcgccgg cgccgccgcc tacgagaagg gccgacccag ctacagcgac
     5761 gacgtggtcg cctggctcgt cgagcgcctg gggatcgcgc ccggccgcac ggtggtcgac
     5821 ctcgccgccg gcaccgggaa gctgacccgc ctcctcgccg ccaccggggc gagcgtcacg
     5881 gccatcgagc cggtcgacgc gatgcgggac cagctcttgc aggcgtgccc cggcgtcgag
     5941 gccgtcagcg ggaccgccga ggcgatcccg ctcccggatc gcaccgtcga cgccctcacg
     6001 gtggcccagg cgttccactg gttcgacccg gcggcggccc tggccgagat cgcccgggtg
     6061 ctccgccccg gcggcgcgct gggcatcgtc ttcaacgagc gcgacacccg cgagccgtgg
     6121 gtg
//
LOCUS       DATMFS010000106         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_166616, whole genome shotgun sequence.
ACCESSION   DATMFS010000106 DATMFS010000000
VERSION     DATMFS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166616"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VNQ33_01975"
     CDS             complement(<1..1232)
                     /locus_tag="VNQ33_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWJ96894.1"
                     /translation="MSPEATPRPQSPWAPQPPGAPASGASYPPHAATQPVAAYVPPTA
                     PPRPLYPSTGTPPVAGGGQPPTWPPAAAPSFGPPPSDPAPNRKARRERNGRGRGLVAV
                     AAVSALLAGAAGGAAGVLAAEHNDRADTAATAVETQPVSTEVPPVKGDSSEPAAAVAT
                     ALGPAVVQIETSKGLGSGFVYDESGLILTASHVTDGESSVEVRMADGTRSTGTVVGED
                     PSTDVAVVRIEPAKDMAVATLATGVTVKVGQTAIAIGSPFGLDQSVTAGIVSAVGRSA
                     ETPGGVIPAIQTDAPINSGNSGGALADRTGRVIGINDSIATGGSNATGNLGIGFAIPI
                     DIAKSVADRLVAGQSTEAGFLGVEGADATGAHTGAAITSVSAGSPADEAGLTESDVVV
                     AVDGKPVSSMIDLAAKVRT"
     gene            complement(1290..>2499)
                     /locus_tag="VNQ33_01980"
     CDS             complement(1290..>2499)
                     /locus_tag="VNQ33_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ96895.1"
                     /translation="FRLTAVYSLLLFGVAAFMLAGMYGFLASRLHDEDVYRTYQITTV
                     QEVPGGVVLTPTEVRASYRSVEALANERALSLLRGYSACALVVMLFVSLVIGWIVAGR
                     VLAPIGRITSVAREIQATDLSRRIALRGPPDELKELADTFDAMITRLDDAFAGQQRFI
                     QEASHELRNPLAVIRTNLDVALSDPDADPAELRRVAELVGASAMRMSRLVDDLLVYAR
                     LGAPAREQAPVELGDLVIDAVDEFQAVAASRGISLVTELAPSCVVAGDALALRQAVAN
                     LLHNAVRLAPESSRIWLATGTEGPAAWVTVTDEGPGIAEEDRPKVFQRFWRGDQKAAR
                     AEGRSGLGLAIVAQIVADHGGTATVAAAPTGGSIFTLWLPLAGTDVTIEATDALAVGH
                     RPADSQEPAS"
BASE COUNT          329 a          854 c          996 g          320 t
ORIGIN      
        1 gtgcggacct tggcggcgag gtcgatcatc gaggagacgg gcttcccgtc gaccgcgacc
       61 accacatcgg actcggtgag gccggcctcg tccgctggcg aaccggccga caccgaggtg
      121 atggcggcgc cggtgtgggc cccggtggcg tcggcgccct cgacgccgag gaacccggct
      181 tcggtggact ggccggccac gaggcggtcg gcgacggact tggcgatgtc gatcgggatg
      241 gcgaagccga tgccgaggtt gccggtggcg ttgctgccac cggtggcgat cgagtcgttg
      301 atcccgatga cgcggccggt ccggtcggcg agcgcaccgc ccgagttgcc cgagttgatc
      361 ggggcgtcgg tctggatggc ggggatgacc ccgccgggcg tctccgccga gcggcccaca
      421 gcgctgacga tgcccgcggt gacgctctgg tcgaggccga acgggctgcc gatggcgatg
      481 gccgtctggc cgaccttcac ggtcacgccg gtggcgaggg tggcgacggc catgtccttg
      541 gccggctcga tcctcacgac ggccacgtcg gtggacgggt cctcgccgac gacggtgccg
      601 gtggatcggg tgccgtccgc catgcggacc tcgacgctcg actcgccgtc ggtgacgtgg
      661 gacgcggtca ggatcaggcc gctctcgtcg tacacgaagc ccgacccgag gcccttcgac
      721 gtctcgatct gcacgacggc tgggccgagc gcggtggcga cggcggcggc cggctcggag
      781 gagtcgccct tcaccggcgg gacctcggtg gagaccggct gggtctcgac ggccgtcgcc
      841 gcggtgtcgg ccctgtcgtt gtgctcggcg gcgagcacgc cggctgcgcc accagccgcg
      901 ccggcgagca gcgcgctcac ggcggcgacg gcgacgagac cccggccccg gccgttgcgc
      961 tcacggcggg ccttgcggtt gggagcgggg tcggacgggg gcgggccgaa cgacggcgct
     1021 gcggccggcg gccaggtcgg gggctgaccg ccaccggcga ccggcggcgt gccggtgctc
     1081 gggtacagcg ggcggggcgg ggcggtcggc gggacgtacg cggcgacggg ctgggtcgcg
     1141 gcgtgcggcg ggtacgacgc gcccgacgcc ggggcgccgg gcggctgcgg cgcccagggg
     1201 ctctgcggac gaggggttgc ttcaggggac atcggacctg cctttcgccg acggaaccta
     1261 cggacgccgc ccgaaaggac ccgtaagcgt cagctggcag gttcctgaga gtccgccgga
     1321 cggtggccga ccgcgagcgc gtccgtcgcc tcgatggtca catcggtgcc ggcgaggggc
     1381 agccagaggg tgaagatcga cccgccggtg ggggccgccg ccaccgtggc cgtgccgccg
     1441 tggtcggcca cgatctgggc cacgatcgcc aggccgagcc cactgcgccc ctcggcgcgc
     1501 gccgccttct ggtcgccgcg ccagaaccgc tggaagacct tgggccggtc ctcttcggcg
     1561 atgcccgggc cttcgtcggt gaccgtcacc caggccgcgg gaccctccgt cccggtggcg
     1621 agccagatgc ggctggactc gggagcgagg cgcacggcgt tgtggagcag gttggccacg
     1681 gcctggcgca gcgcgagggc atcgcccgcc accacgcacg acggggccag ctcggtcacg
     1741 agggagatgc cgcgggaggc ggcgacggct tggaactcgt cgacggcatc gatcaccagg
     1801 tcgccgagct cgacgggcgc ctgctcccgc gccggtgcgc cgagccgggc gtagaccagc
     1861 aggtcgtcga cgaggcgcga catccgcatc gcggacgcgc ccaccagctc ggcgacgcgc
     1921 cgcagctcgg ccgggtcggc atcggggtcc gacagggcga cgtcgaggtt ggtgcggatg
     1981 acggccagcg ggttgcggag ctcgtgcgac gcctcctgga tgaagcgctg ctggccggcg
     2041 aaggcatcgt cgagccgggt gatcatggcg tcgaacgtgt cggcgagctc cttcagctca
     2101 tcgggcggcc cgcggagcgc gatgcgccgc gacaggtccg tggcctggat ctcccgggcg
     2161 acgctggtga tgcgcccgat gggagcgagc acccgtcccg cgacgatcca gccgatgacg
     2221 aggctgacga agagcatcac gacgagggcg cacgccgagt acccgcgcag caaggagagc
     2281 gcccgctcgt tcgccagcgc ctcgaccgac cggtagctgg cccggacctc cgtgggcgtg
     2341 agcaccacgc cgccggggac ctcctggacc gtggtgatct ggtacgtgcg gtacacgtcc
     2401 tcgtcgtgca gccggctggc caagaagccg tacatgccgg cgagcatgaa cgcggcgacg
     2461 ccgaacagga gcagcgagta gacggcggtg agccggaag
//
LOCUS       DATMFS010000107         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_190685, whole genome shotgun sequence.
ACCESSION   DATMFS010000107 DATMFS010000000
VERSION     DATMFS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190685"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VNQ33_01985"
     CDS             complement(<1..542)
                     /locus_tag="VNQ33_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ96896.1"
                     /translation="MRVLITGMGGELGTRVANLMEADESVTEIMGMDLFPPRRRIFRS
                     EFHRVEPTDRRKAVHLVRDFDPEVVLHLGVFEPHSRTSPSEARRGTAASAVSVLGAAA
                     RCPSLQAVVVRSGIEVYGRSRGASTRPDESVPIQPTSPFGESLAHVEQVAVEAGDVAG
                     VPVTALRYAPIVGPSIPSPLG"
     gene            complement(562..2037)
                     /gene="lysS"
                     /locus_tag="VNQ33_01990"
     CDS             complement(562..2037)
                     /gene="lysS"
                     /locus_tag="VNQ33_01990"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWJ96897.1"
                     /translation="MNDRPSVPYRFDPDATAAGLKQAYGDLEPGIETGDRVVVAGRLM
                     LRRGQGKLAFGQLADSTGRIQLFATANDTPEFERFGKLSLGDWIGVTGEVMTTRKGEL
                     SVKVLEWAVLAETRRPFPDKWHGITDPDTRYRQRYVDLWVTEEARLTLQARSKVVSLV
                     RRWLEDRGFMEVETPALHPVPGGALARPFTTHHNALDQEMFLRIAPELYLKRLVVGGF
                     ERVFEIARVFRNEGISPRHNPEFTMLELYQAYADWTDIMELVEQLVAYLAVEICGSTT
                     VTYQDRELDLSVPWRRASMASLIEEQIGRTIGLDTPLGELRALCDELGVEWKDSYGAG
                     KLMLEIYEKTAEGQQWNPVFVTEYPIEVSPLARPHRELPGMTERFEGIVAGRELCNAF
                     SELLDPDDQRARFEDQVRERDNGDEEAMAVDEDYLRALEYGLPATGGLGIGIDRLVML
                     LTDSSTIRDVVLFPTLRPEQDLASGTMVDGGEDGSGQSSST"
     gene            2138..>2840
                     /locus_tag="VNQ33_01995"
     CDS             2138..>2840
                     /locus_tag="VNQ33_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWJ96898.1"
                     /translation="MGTAKTGIIGSGAAAAALLLLAGAAPPAGAAPVAPSTVVRTCPA
                     PFVTADPVWSTTPGVPAGNPGVAIDQVPAPARTQTALDMDDDGTVDTTVVGLDGSVTV
                     QRGDGDLVVTGIPSGGTGFSVTGDDLDGDGRSELVVWRRTGIGQGTRSTTWIVPGTTP
                     PGTVAATDVGASFEGGVTGDVTGDGLDDLSVAIPAILVSIPLGYLSGADVVAALPSSS
                     PFAGLDVIALRADLDF"
BASE COUNT          386 a          981 c         1061 g          412 t
ORIGIN      
        1 ccgagcgggc tcgggatgct ggggccgacg atgggggcgt agcgcagggc ggtgacgggc
       61 acgccggcca cgtcgcccgc ctccacggcg acctgctcca cgtgggcgag cgactcgccg
      121 aagggggagg tcggctggat cggcacggac tcgtcgggac gggtggaggc gccccggctg
      181 cgtccgtaga cctcgatgcc ggagcggacg acgacggcct ggagcgacgg gcacctggcg
      241 gcggcgccga gcacggagac ggcagaggcg gccgtgcccc gacgggcttc gctggggctg
      301 gtgcgcgagt ggggctcgaa cacgccgagg tggagcacga cctccgggtc gaagtcccgg
      361 acgaggtgca cggccttgcg gcggtcggtg ggctcgaccc ggtggaactc ggagcggaag
      421 atgcggcggc ggggcgggaa caggtccatg cccatgatct cggtgaccga ctcgtcggcc
      481 tccatgaggt tggcgacgcg agtgcccagc tcgccaccca tcccggtgat cagaaccctc
      541 atgccttgtc tctcccccgg atcacgtgct gctggactgc ccgcttccgt cttcgccccc
      601 gtccaccatg gtgccagatg cgaggtcctg ctccggacga agcgtcggga agagcaccac
      661 gtcacggatg gtggacgagt cggtgaggag catgaccagg cggtcgatgc cgatgccgag
      721 gccgccggtg gcgggcaggc cgtactcgag cgcacggagg tagtcctcgt cgacggccat
      781 ggcctcttcg tcgccgttgt cgcgctcgcg gacctggtcc tcgaagcggg cgcgctggtc
      841 gtcggggtcg agcagctcgc tgaacgcgtt gcacagctcc cggccggcga cgatgccctc
      901 gaaccgctcg gtcatgccgg gcagctcgcg gtggggccgg gcgaggggcg acacctcgat
      961 ggggtactcg gtgacgaaga ccgggttcca ctgctggccc tcggccgtct tctcgtagat
     1021 ctcgagcatg agcttcccgg cgccgtagct gtccttccac tcgacgccga gctcgtcgca
     1081 cagggcgcgc agctcgccga ggggcgtgtc gaggccgatg gtccggccga tctgctcctc
     1141 gatgagcgag gccatcgacg cccggcgcca cggcaccgac agatccagct cgcggtcctg
     1201 gtaggtgacg gtggtggacc cgcagatctc gacggcgagg taggccacga gctgctcgac
     1261 cagctccatg atgtcggtcc agtcggcgta cgcctggtac agctcgagca tcgtgaactc
     1321 ggggttgtgg cggggcgaga tgccctcgtt gcggaagacg cgggcgatct cgaacacccg
     1381 ctcgaagccg ccgaccacca gccgcttgag gtacagctca ggggcgatgc gcaggaacat
     1441 ctcctggtcg agcgcgttgt ggtgcgtggt gaacgggcgg gccagcgcac cgccgggcac
     1501 ggggtgcagc gcgggggtct cgacctccat gaagccgcgg tcctcgagcc agcggcgcac
     1561 gagcgacacg accttggagc gggcctgcag cgtgagccgg gcctcctcgg tgacccacag
     1621 gtcgacgtag cgctggcggt agcgggtgtc ggggtcggtg atgccgtgcc acttgtcggg
     1681 gaacgggcgg cgggtctcgg ccagcacggc ccactccagg accttgacgg acagctcgcc
     1741 cttgcgggtg gtcatgacct cgccggtgac accgatccag tcacccagcg acagcttccc
     1801 gaaccgctcg aactcgggcg tgtcgttggc ggtggcgaac agctggatgc ggcccgtgga
     1861 gtcggccagc tggccgaacg ccagcttgcc ctggccccgg cggagcatga ggcgcccggc
     1921 gaccaccacc cgatcgcccg tctcgatgcc gggctcgagg tcgccgtagg cctgcttgag
     1981 cccggcggcg gtggcgtcgg gatcgaagcg gtagggcacg gacggacggt cgttcacggg
     2041 gttcctcgtc gacgggcgca agagcgcggg accatccagc ctgcccgctg gaccgcgttg
     2101 gggcccaacc tttcggcggt gtgagtaggt tgcgcccatg gggacggcga agacggggat
     2161 catcggttcg ggcgccgccg ccgcggcgct cctcctgctg gcgggggcgg ctccgccggc
     2221 tggcgccgca ccggtggctc cctcgaccgt cgtccgcacc tgtccggcgc ccttcgtcac
     2281 cgcggacccg gtgtggagca ccacgcctgg ggtgccggcg gggaaccccg gtgtcgccat
     2341 cgaccaggtc cccgctcccg cccgaaccca gaccgcgctg gacatggacg acgacgggac
     2401 cgtcgacacg accgtggtcg gactcgacgg atccgtcacc gtccagcgcg gcgacggcga
     2461 tctcgtggtg accggcatcc cgtccggggg caccggcttc tccgtcacgg gcgacgacct
     2521 cgacggcgac ggccgcagcg agctcgtcgt ctggcgccga accggcatcg gccagggaac
     2581 gcggagcacc acgtggatcg tgcccgggac cacccctccc ggcacggtcg cggccaccga
     2641 cgtcggggcg tcgttcgagg gcggggtgac gggcgacgtg accggcgacg gcctcgacga
     2701 cctctccgtc gccatccccg ccatcctggt ctcgatcccg ctgggctacc tctcgggcgc
     2761 cgatgtcgtc gccgcgctgc cctcgtcgtc gccgttcgcc gggctcgacg tcatcgccct
     2821 gcgggcggac ctggacttcg
//
LOCUS       DATMFS010000108         6831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_306335, whole genome shotgun sequence.
ACCESSION   DATMFS010000108 DATMFS010000000
VERSION     DATMFS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306335"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="VNQ33_02000"
     CDS             complement(<1..1758)
                     /locus_tag="VNQ33_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96899.1"
                     /translation="MTGGVASCWGSNRSGQLGNGTYHHASAPTPVTVSGPLVGAPVAQ
                     VAVGEGVACALTTVGAVACWGGDGYSKTGNAATTDHAALPVPVATNGPLQGRTVTQIS
                     AKRWHVCALTADSAIVCWGFNASGQLGNGNRTDQAVPVAVVVAGGPLAGKTIASIDVG
                     YSHSCALATDGTIGCWGSNSDGQLGNGTTTDSAVPVAVKTMDTALEGKTPVEVSVGQL
                     HTCARTSDGSVACWGSGIGGALGRGNTASSKVAVPVKTTTALAGKTVADVEAGGYFTC
                     VRTTDGLPICWGTGTYGQQGNGLTTGEGDPTIVTVAGTALEDRSVNDLAVGLTHSCAV
                     SVEVVACWGQNKYGQIGNGVSGVALVPAPAVNGALTGATVDQIAGGPTNGCARTSAGV
                     ACWGENGAHQVEFLDLVDRRSAVLINTGGSPLSGSPAVDLAVGAQHACAVSADGQVTC
                     WGSNGSKQLGRSGYFGYPLAVANDSGTGLYEKHAVRVVAGTASSCALTSDGVVVCWGG
                     NAAGQLGNGTTKSTFVPTAITTAGTPLAGKTVVDLAYGDDQGCALASDSTLACWGINT
                     LGALGNGSTVNSLVPVAVTR"
     gene            complement(2142..4805)
                     /locus_tag="VNQ33_02005"
     CDS             complement(2142..4805)
                     /locus_tag="VNQ33_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96900.1"
                     /translation="MGNAVTQQISWKRLEEIPVASGLTFYERERDLFLSLESPIWPSD
                     EVNPPGWNTRTDIAYEGTTYRVLERQILSTSDADARRIRSAGEKAYAAHQRAAKEAGE
                     RPQKAEDFLIDIEGNPVLRERLLRFPSVPIYGLGPASTLPHRAPAGFHSFRVLDHPER
                     TGSVGRVYLLQHGLNEIPGAELFYRLAGWILAGDPDAACIVRPFPGHLTRYSVNSRFA
                     ETPLDRYLEDSAELFRHYLRFMIETRWFLSTIAPRNRYSSLAGAGLVGTREVGETDEE
                     SRSDLGTLARSVSAEYASIRDAVASRSVNADRLPLPPTVASIEDSLGALRAVLGWNPT
                     TKPAVPNPVAAEAPTVHAIGYSLGGFVSQSVFMTWPYVVSTCTTICSGGALRDLAPTA
                     FAHPEEWQTLVHSLRYQLDRLMSDGELAARDGDPVLGMDGELYDYLLRIFYEVFEQDY
                     RSSYQSRVSEYLRRIWFIVGGNDPIVRPQSVLDAAPAEGANIFSIADMSHFLSTARLA
                     RHPGETEQREFWLPQLGGILPAFAQEGEYSAQRSLANGWRKDDNSAYSNGSKPRKLAS
                     AIDESERLLAGVDGSLASPTFERFLDALCAKASETGHLFIFRNDIPTFMLSEEYQVYR
                     AKSLHHSDDKVRAYLEGLERRRAALLGVEGESEGMGRRLTVVLPSDAVDRLGRSFRPG
                     MAQPTSEAPMGGFRLPKGVKKSDLAKSEREGFLDRLASEPGSNDPNVWRFTPGPIAVD
                     GSDGIIDEALRIEAIRETIPFLKEDSFKGQEQLAVTRLPDLWLYVSRDALLPSSQGRI
                     GGEVALISSFARIFTDKAFEKDKRNSSIPVEQPVEWIQKDQIRGLVVSRAKNNPRHRG
                     RTILERREFGRLAVHASVALLRSERWVEGGS"
     gene            complement(4990..5415)
                     /locus_tag="VNQ33_02010"
     CDS             complement(4990..5415)
                     /locus_tag="VNQ33_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96901.1"
                     /translation="MVVETNPGQTTLVWSTHLAPSQVAELFGPIARGAGTEVSLVSVA
                     SNGFSTRVEGSEDVQLGLKQIQTETATTSLFRLEVLDKGIGDVYLAYSQPSGAPGTLG
                     SVSMMAADVNHDCLRKVFVESADGDMCPLFDDIAQHLSA"
     gene            6214..6504
                     /locus_tag="VNQ33_02015"
     CDS             6214..6504
                     /locus_tag="VNQ33_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96902.1"
                     /translation="MEDLAQVHTTYQGHHRELCVHEIGIGPKGNEQILAYQFGGTSSK
                     GLASAMDEKDRWRCLDIALMQDIAVVAGPWHTGTNRGPGNYCIEITDLVIHP"
     gene            complement(6540..6758)
                     /locus_tag="VNQ33_02020"
     CDS             complement(6540..6758)
                     /locus_tag="VNQ33_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96903.1"
                     /translation="MLAWRPDDEGRLLGQQYFSEQFAVSARVRTWYWSLHCGSVSATE
                     RKTSVRLSLECLYLPLAVVSRRGVQVAP"
BASE COUNT         1141 a         2400 c         2217 g         1073 t
ORIGIN      
        1 acgggtcacc gccactggca ccagcgagtt gacagtcgac ccgttgccga gcgcacccaa
       61 cgtgttgatg ccccagcacg ccagcgtgct gtccgacgcg agcgcacagc cctggtcgtc
      121 gccgtaggcg agatcgacca cggtcttgcc ggcgagcggg gtgccggccg tggtgatcgc
      181 ggtcggaacg aacgtgctct tggtcgtacc gtttccgagc tgcccagcgg cgttcccacc
      241 ccagcagacg acaaccccgt ccgaagtgag ggcgcagctc gacgccgtac cagccaccac
      301 ccgaacggcg tgcttctcgt acaggccggt gccgctgtcg ttcgcgacgg cgagcgggta
      361 gccgaagtag ccgctgcggc ccagctgctt ggacccgttg ctgccccagc acgtgacctg
      421 tccgtcggcc gacaccgcgc aggcgtgttg ggcgccgact gccaggtcga ccgcgggcga
      481 tcccgacaac ggcgagcctc cggtgttgat cagcacggcc gaccggcggt ccacgaggtc
      541 gaggaactcg acctggtgcg cgccgttctc cccccagcag gccacgccgg cagacgtgcg
      601 ggcgcagccg ttggtcggtc cgccggcgat ctggtccacg gtcgcgccgg tgagcgctcc
      661 gttcacggcc ggagcgggca cgagggccac acccgacacc ccgttgccga tctggccgta
      721 cttgttctgc ccccagcacg ccacgacctc gaccgacacg gcacagctgt gggtcaggcc
      781 gacggccagg tcgttgaccg accggtcctc cagcgcggtc ccggcaacgg tgacgatcgt
      841 cggatcgccc tcgcccgtgg tgagcccgtt gccctgctgc ccgtaggttc cggtgcccca
      901 gcagatcggc aggccatcgg tcgtccggac gcacgtgaag tacccgccgg cctccacgtc
      961 ggcgacggtc ttgccggcca gcgccgtggt ggtcttcacg gggacggcca ccttcgagct
     1021 ggccgtgttg ccccgaccta gcgctccgcc gataccggac ccccaacagg caacgctgcc
     1081 gtcggacgtc cgggcgcagg tgtgcagctg gccgaccgag acctcaaccg gcgtcttgcc
     1141 ctcgagcgcc gtgtccatcg tcttgacggc gaccggcacc gccgagtcgg tcgtggtccc
     1201 gttgccgagc tggccgtcgc tgttcgatcc ccagcatccg atcgtcccgt ccgtcgcgag
     1261 cgcgcacgag tggctgtagc cgacgtcgat cgacgcgatc gtcttcccgg cgagcggccc
     1321 gccggccacg acgaccgcca ccggcaccgc ctggtcggtc cggttgccgt tgcccagctg
     1381 accggaggcg ttgaagcccc agcagacgat ggcgctgtcc gccgtcaggg cgcagacgtg
     1441 ccagcgcttg gcagagatct gggtgacggt ccggccctgc aaggggccgt tggtggcgac
     1501 gggcacgggc agggcggcgt gatcggtggt cgcggcgttg ccggtcttcg agtacccgtc
     1561 gccgccccag caggcgacgg cgccgacggt cgtcagagcg caagcgacgc cttcaccgac
     1621 cgccacttgc gccacgggtg cgccgacgag cggacccgac accgtgaccg gggtgggggc
     1681 cgacgcgtgg tggtaggtgc cgttgccgag ctgacccgag cggttgctcc cccagcacga
     1741 cgccacgccc ccggtcacca ggcagctgtt gtcgccgccg gtcgcgatcg acgcgaccgg
     1801 cgcgggatcg cccgccgccg cgccgctgcg gacgacaggt gccggggcgg tcgggctggc
     1861 cgctctcccc gacgccggcg aagccgctac gaccgcgatg agaaccaccg cccccagccc
     1921 tgcgacacca cgaaccaacc gcccacgatc ggaccgcatg cgactctccc tccccgggct
     1981 ggctcccctg tcaacccggg gcaggacact acccgtgccg gcgttctggc ggtgttgtcg
     2041 gtcgatggcc acgccgccaa ggagacatca gcagaacccg gcgaccgtgc ccggcgaccg
     2101 gccccgttcg acgtcaggcg gttgggccgg cgaacgaggg ctcaggatcc gccttcgacc
     2161 cagcgctcgg accgcagcag cgcgaccgac gcatggacgg ccaggcgacc gaactcccgg
     2221 cgttccagaa tggtgcgtcc acgatgccgg gggttgttct tcgcccgcga gaccacgagc
     2281 ccccggatct ggtccttctg gatccactcc accggttgct ccacggggat ggagctgttc
     2341 cgcttgtcct tctcgaaggc cttgtcggtg aagatgcggg cgaagctgga gatgagcgcc
     2401 acctcgccgc cgatcctccc ctgcgaggac ggtaggagcg catcgcggct cacgtagagc
     2461 cagagatccg gcaggcgggt gactgcaagc tgctcctggc ccttgaagct gtcttctttg
     2521 aggaacggga tcgtctcccg gatcgcctcg atgcgcagtg cctcgtcgat gatgccgtcg
     2581 gacccgtcca ccgcgatcgg ccctggggtg aaccgccaca cgttcggatc gttgctgccc
     2641 ggttctgagg ccaaccggtc caagaagccc tcgcgctccg acttcgcaag gtcggacttc
     2701 ttcactccct tgggaagtcg gaaccctccc atcggggcct cgctcgtcgg ctgcgccata
     2761 cccgggcgga agctccggcc gagtcggtct acagcgtcgg acggaagcac cacggtgagc
     2821 cgacgtccca tgccctcgga ctcgccctcc actccgagaa gcgcagcacg ccggcgctcg
     2881 agaccctcga ggtacgcacg caccttgtca tcgctgtggt gcagcgactt cgcccggtac
     2941 acctggtact cctccgagag catgaacgtc ggaatgtcgt tgcggaagat gaagaggtgc
     3001 cctgtctcgg atgccttggc acagagcgcg tcgaggaagc gctcgaacgt cggggaagca
     3061 agcgacccgt ccacgcccgc aaggaggcgc tcggactcgt cgatcgcact cgcgagcttc
     3121 cgcggcttgg acccgttcga gtacgcggag ttgtcgtctt tccgccaacc gttcgcgagc
     3181 gagcgctgcg cggagtactc cccttcttgc gcgaaggccg gcaggatgcc gccgagttgc
     3241 ggcagccaga actcccgctg ctcggtctca cccgggtgcc gcgccaggcg agcagtgctg
     3301 aggaagtgcg acatatccgc gatcgagaag atgttggcgc cctcggcggg cgccgcatcg
     3361 agcacggact gcggcctcac gatgggatcg ttcccgccca cgatgaacca gatccggcgg
     3421 aggtactccg acacccggct ctggtacgaa gagcggtagt cctgctcgaa aacctcgtaa
     3481 aagatgcgca ggaggtagtc gtacaactcg ccgtccatgc ccaagacggg gtcgccgtct
     3541 cgcgctgcca gctcgccgtc ggacatcagg cgatcgagct ggtaccgcaa tgagtggacg
     3601 agggtctgcc actcctcagg atgggcgaac gcggtaggcg cgagatcccg gagcgctccg
     3661 cccgagcaga tcgtggtgca ggtggagacg acgtagggcc aggtcatgaa gaccgactgg
     3721 gagacgaacc cacccagcga gtagccgatg gcgtgcactg tcggtgcttc ggccgcgacc
     3781 gggttcggga ccgcaggctt ggtcgtcggg ttccacccga gcacagctcg cagcgccccg
     3841 agcgagtcct cgatggatgc cacggtgggc gggagtggaa gccgatccgc gttgacgctc
     3901 cggctcgcaa ccgcgtcccg tatcgacgcg tattcggccg agaccgaccg cgcgagggtc
     3961 ccgaggtcag agcgcgactc ctcgtccgtc tcaccgactt cacgggttcc caccagaccc
     4021 gcacctgcca gcgatgagta gcggttccgc ggtgcgatcg tcgagaggaa ccaacgggtc
     4081 tcgatcatga acctcaggta gtgccggaag agctcggcgc tgtcctccag gtaccgatcg
     4141 aggggcgtct cggcgaatcg cgagttcacg ctgtaacgag tgaggtgacc agggaagggc
     4201 cggacgatgc acgcggcgtc cggatcaccg gcgaggatcc aaccggccaa ccggtagaag
     4261 agctcggcac ccgggatctc gttgaggccg tgctggagca ggtagacccg gccgaccgag
     4321 cctgtccgct ccggatggtc gagcacgcga aacgagtgga acccagcggg cgcccgatgc
     4381 ggcagggtcg aggccggacc caagccgtag atcgggaccg aagggaagcg gagcagccgc
     4441 tctcgcagga ctgggtttcc ttcgatgtcg atcaggaaat cctcggcctt ctgcggtcgt
     4501 tctcctgctt ccttcgcggc gcgctgatga gcggcatatg ccttctcgcc agccgaccga
     4561 atccgccgag catccgcatc ggacgtactg aggatctgac gttccagaac ccggtacgtg
     4621 gtgccctcgt aggcgatgtc cgttcgggtg ttccatcccg gcgggttcac ctcatcagac
     4681 ggccagatcg ggctctcgag gctgagaaag agatcccgct ctcgctcata aaaggtcagc
     4741 ccgctggcaa ccgggatttc ctcaagacgc ttccatgaga tctgttgggt caccgcgttc
     4801 cccactatcg cgaactattt ccgagccgga gggcgaaacg cgacgcaatc caccgccatc
     4861 gaggggtttc gactctcgcc acgatgcccc tccccacccg tggcgtaccg taggccaccc
     4921 ccccgttgcc acgcaactgg tcaggcggcg agccgtgtga ccgctacggc gatcacccgg
     4981 tcgaggtcgt caggcagata ggtgttgtgc gatgtcgtcg aacagcgggc acatgtcgcc
     5041 atctgccgac tccacaaaga ccttgcgcag gcagtcatga ttgacgtcag ccgccatcat
     5101 cgacaccgac ccgagcgtcc ctggagctcc acttggctgg ctgtaggcca aatagacatc
     5161 gccgatgccc ttgtcgagga cctcgagccg gaagagcgac gtcgtcgccg tttccgtctg
     5221 gatctgcttc aggccgagtt gcacatcctc gcttccctca acccgcgtcg agaagccgtt
     5281 ggatgccacg ctgaccagcg agacctccgt gcccgctccg cgagcgatcg ggccgaagag
     5341 ctcggcgacc tgcgagggcg caagatgggt gctccagacg agggtggtct gccctgggtt
     5401 ggtctcgacg accacggccc cagaggctcc gtcacacctc cgtacgttcg acgacacccg
     5461 acgccagttc gccgtcaacg agctcatcgc actctcccca cccacgccca tgagaaccgg
     5521 ctcaacacta gcagagcgat cggtgcccgc caagggttcc ggggggtttg aggtcgacat
     5581 cgggctctcg ttcttcgtag tggtctctag ctgactgccc gcgcctgcac cgccacagta
     5641 gcgaggtcag gccccgccgc gaccgcgtca cggtggaacg caccatgacc atcggcacag
     5701 caacggacaa actagagcaa tcgttgtctg acccatccgc gtctttctcg gacgcctctg
     5761 tgcgcccccc gcctgatgga ccggccccag cgcgcctgat ggaccggcgc cagcgccctc
     5821 gccccacggc cacgggcgcc atctcgagcc gccggtctcg gccatggggc tccctttggc
     5881 gctccggcac cgcgccggcg ttcatttcga acggaacacg cgcggctccc gtgccgctga
     5941 gaacgggcgc gaaatggtca acgtgctgtg aacggtgcag gcgatgacca gaacatgtct
     6001 cccctcacct accgaccgtg ttcaacgatg atcagggacg ggcttgggag cggaagtagc
     6061 caaagcctcg gcggctcagc aatcacgcgc tgacaaaggt acggtcgcgt ggctcctacc
     6121 gggtacggcg gtctcggagc atccacaccc ttggacacct gccccggagg cggaccaatg
     6181 ccctcatcga gctaccagac cattcggcaa gccatggagg accttgcgca ggttcacacg
     6241 acttaccagg gccaccatcg cgaactgtgc gtccacgaga tcggtatcgg cccgaagggg
     6301 aacgagcaga ttctcgccta ccagttcggt ggcacctcgt cgaagggcct cgcaagcgcc
     6361 atggatgaga aggaccgctg gaggtgcctt gacatcgccc tgatgcagga catcgctgtt
     6421 gttgcaggcc catggcacac cgggaccaat cgagggccgg gcaactactg catcgagatc
     6481 accgacctgg tgattcaccc gtaaccctga cccggcgggg cgtcggctgg tggccggttt
     6541 cacggggcca cctgcacgcc gcgcctcgac accaccgcca agggcaagta caagcactcc
     6601 aagctcaacc ggaccgacgt cttccgctcg gtggccgaga ccgatccgca gtggagcgac
     6661 cagtaccacg ttcgaacccg ggccgagacg gcgaactgct cactgaagta ctgctggccc
     6721 aacaagcgac cctcgtcgtc ggggcgccac gccagcacct cgacctgctg ctcgctggcc
     6781 tccgggcaac ctcatcgctt gaaccgggtc ggaaccccgg tcctttggac c
//
LOCUS       DATMFS010000109         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_283453, whole genome shotgun sequence.
ACCESSION   DATMFS010000109 DATMFS010000000
VERSION     DATMFS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283453"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VNQ33_02025"
     CDS             complement(<1..744)
                     /locus_tag="VNQ33_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96904.1"
                     /translation="MTVDVGDERRLQAAGATIAGELPEAVREMARAYPYSWGGPYRRY
                     GYDVAADLTEIETEALVSGTGSALLTIGISEPVAALGWERMAEESAHFGITVARAGSL
                     LTAPSADRRALGRDLFAAVRQQAGAGLIVAEVDTRDLEGLAAVVEAGFVPVDTSLTYI
                     GDADEGPGNLHPSHGLSVDIGSPATVAFTDDEVALSVEVARASLHLDHWHSDPRVDPE
                     RASEWYATWVQRAFEGTWADVIILPRRDGV"
     gene            890..2518
                     /locus_tag="VNQ33_02030"
     CDS             890..2518
                     /locus_tag="VNQ33_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ96905.1"
                     /translation="MARVALCQDVLVEYAGYMSISAVLKEGGHTVELFFDDQRHPDRF
                     LRKLADFRPDVVCFSLLSPSVPWAQRTARRVSDEIGALTIAGNVHVIMNPDTIIEEGS
                     FDVIATGEGERCLLELCETLDRGGDHSAIDGLWVRTADGIRRNPSAPLVDMDELPFMD
                     RDLYDKYAFFRQSAYLRVCCGRGCPFRCSFCSNTNLTDHYGGAKTYLRKRNPTLAVDE
                     IVHLLQGRPKVSSIFILDEVLWFDRKWLYEFLEQYRDRVGLPFTANLKFNGVREEDIR
                     RLAEAGVHGMAVAAETADEEQRRGLMNKPVSNTHILQITEWMHEHGVKFANSVFFGLP
                     GDRFEDHIERLPFFRQTNPDYLWTTFFQPYPGLNILDNDEIRASIPDGREFDSTLHHD
                     MYLDLEDRDRLVNLKKVYFLMMRFPRTERPLAFLCQYRIPVLFDLLFLSHFAWYALKY
                     ENLSAYQMLHHVKNMGINPILRRAQPLPGIGRPFGLRYRNTALRKIRARERAEKRRAE
                     REARVAPAPSVSVPVALTARRPTEGRGADAPVSA"
     gene            2566..3246
                     /locus_tag="VNQ33_02035"
     CDS             2566..3246
                     /locus_tag="VNQ33_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ96906.1"
                     /translation="MSQLVNEGNPAASSGPRPSTPEDERGSIWSSLSYPPLYRFLQRI
                     SGSDHARRVFADEILRCGPEERILDIGCGTAELIELLPPTVSYLGLDSRARYIDHARR
                     CYGDRATFLQGTIEELAVDDVGPRDIVLAGAVMHHLDDQTVDDLLETARAVLVPGGRF
                     VAGDPVILPVQHPIARTVARYDRGHHVRTVEETEALVGRHFPDASFTVRSDLQRFPYD
                     HVIVDARR"
     gene            3243..>3448
                     /locus_tag="VNQ33_02040"
     CDS             3243..>3448
                     /locus_tag="VNQ33_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96907.1"
                     /translation="MTTLPTDVEATGTAEAAEGHGPTGPDTPAPYRGAWASAAAGVAM
                     VVMAPVFIYLVDHPTVPFFGVHDF"
BASE COUNT          532 a         1309 c         1099 g          508 t
ORIGIN      
        1 cacgccgtcg cgccgcggga ggatgatcac atcagcccac gtcccctcga acgcccgctg
       61 cacccaggtg gcgtaccact ccgaggcccg ctcgggatcc acgcggggat cgctgtgcca
      121 gtggtcgagg tgcaacgagg cacgggcgac ctccaccgag agcgcgacct cgtcgtcggt
      181 gaacgccacc gtggcgggtg agccgatgtc gacgctcagc ccgtgcgacg ggtgcaggtt
      241 ccccggccct tcgtcggcgt cgccgatgta ggtgagcgag gtgtccacgg gcacgaagcc
      301 ggcttcgacc accgcggcga ggccctcgag gtctcgggtg tcgacctcgg ccacgatcag
      361 cccggcgccg gcctgttgac ggaccgccgc gaacaggtcg cgcccgagcg cccgccggtc
      421 ggcggacggc gcggtcagca gcgagcccgc cctggcgacg gtgatgccga agtgggcgct
      481 ctcctccgcc atgcgctccc agccgagggc ggcgaccggc tcggagatcc cgatggtgag
      541 gagggccgag ccggtaccgg acaccagggc ctcggtctcg atctcggtga gatcggcggc
      601 gacgtcgtag ccgtagcggc ggtagggacc gccccaggag tacggatagg cacgcgccat
      661 ctcccgcacg gcctccggaa gctcaccagc gatcgtggca cccgcagcct ggagacggcg
      721 ctcgtccccc acgtcgaccg tcacatgctg gtgctaccac gggctcagcc cggtcgtcaa
      781 cgccgttggc cgggccagcc gaccaacacc tgggtcgtgg tagcttcgat gcggctgcga
      841 gcggacgagg tccgatcacg cgcccgaccg tccgccgaga gggaagcaca tggctcgagt
      901 cgcgctctgc caggacgtac tcgtcgagta cgcgggctac atgtcgatct cggccgtcct
      961 caaggagggc gggcacacgg tcgagctgtt cttcgacgac caacgccacc cggaccgctt
     1021 cctccgcaag ctggccgact tccggcccga cgtcgtctgc ttctcgctcc tctcccccag
     1081 tgtgccgtgg gcccagcgca ccgcccgtcg ggtcagcgac gagatcggcg ccctcaccat
     1141 cgccggcaac gtccacgtga tcatgaaccc cgacacgatc atcgaggagg ggtccttcga
     1201 cgtcatcgcc accggcgagg gggagcgctg cctcctcgag ctgtgcgaga cgctcgaccg
     1261 gggcggggac cacagcgcca tcgacggctt gtgggtccgc acggccgatg gcatccgcag
     1321 aaacccgtcc gccccgctgg tcgacatgga cgagctgccg ttcatggacc gcgacctgta
     1381 cgacaagtac gccttcttcc gccagagcgc ctacctgcgc gtctgctgcg ggcgcgggtg
     1441 cccgttccgc tgctcgttct gctcgaacac caacctcacc gaccactacg gcggcgccaa
     1501 gacctacctc cgcaagcgca acccgaccct cgccgtcgac gagatcgtcc acctgctgca
     1561 gggtcggccc aaggtctcgt cgatcttcat cctcgacgag gtgctgtggt tcgaccggaa
     1621 gtggctctac gagttcctcg agcagtaccg ggaccgggtc gggctgccgt tcacggcgaa
     1681 cctcaagttc aacggcgtcc gcgaagagga catccgccgc ctcgccgagg ccggcgtcca
     1741 cgggatggcc gtcgccgccg agaccgcgga cgaagaacag cgccgggggc tgatgaacaa
     1801 gcccgtgtcc aacacacaca tcctccagat caccgagtgg atgcacgagc acggcgtgaa
     1861 gttcgccaac agcgtcttct tcggcctgcc gggcgaccgc ttcgaggacc acatcgagcg
     1921 gctcccgttc ttccggcaga ccaaccccga ctacctgtgg acgacgttct tccagcccta
     1981 tcccgggctg aacatcctcg acaacgacga gatccgagcc tcgatccccg acggccggga
     2041 gttcgactcc acgctgcacc acgacatgta cctcgacctc gaggaccggg accggctggt
     2101 caacctcaag aaggtctact tcttgatgat gcggttcccc cgcaccgagc ggccgctcgc
     2161 cttcttgtgc cagtaccgga tcccggtgct cttcgacctc ctgttcctca gccacttcgc
     2221 ctggtacgcg ctcaagtacg agaacctgtc cgcgtaccag atgctccacc acgtcaagaa
     2281 catgggaatc aacccgatcc tccggcgcgc ccagccgctg ccgggcatcg ggcggccgtt
     2341 cggcctgcgc taccgcaaca ccgcgctccg caagatccga gcccgcgaac gagcggagaa
     2401 gcgccgtgcg gagcgcgagg cgcgcgtcgc acctgccccg agcgtctcgg tcccggtcgc
     2461 gctgaccgcc cgccgcccca ccgaaggtcg tggcgccgac gcccccgtgt cggcctagga
     2521 ccgggtgtgt gccccgcgcc cgagccgggt cctagggtgc tggacgtgtc gcagctggtg
     2581 aacgagggga accccgcggc ctcgtccggg ccgcggccga gcacgcccga ggacgagcgc
     2641 ggcagcatct ggtcgtccct gtcctaccca ccgctctacc ggttcctgca gcgcatctcc
     2701 ggctcggatc acgctcggcg cgtcttcgcc gacgagatcc tgcgctgcgg ccccgaggag
     2761 cggatcctcg acatcgggtg cggcacggcg gagctcatcg agctcctccc tccaaccgtc
     2821 tcctacctgg gcctggactc ccgcgcccgc tacatcgacc acgctcggcg ctgctacggc
     2881 gatcgagcca ccttcctcca ggggaccatc gaggagctcg ccgtggacga cgtcggcccg
     2941 cgcgacatcg tcctcgccgg cgcggtgatg caccacctcg acgaccagac ggtcgacgac
     3001 ctgctggaga cggctcgggc ggtcctggtc ccaggcggcc gcttcgtcgc cggcgacccc
     3061 gtgatcctcc ccgtccagca ccccatcgcc cgcacggtgg cccgctacga ccgcgggcac
     3121 cacgtccgca ccgtcgagga gacggaggcg ctcgtgggcc gccacttccc cgacgcgtcg
     3181 ttcaccgtcc gatcggacct ccagcgcttc ccgtacgacc acgtcatcgt cgacgcccgg
     3241 cggtgaccac cctgccgacc gacgtcgagg cgaccggcac ggccgaggcc gccgaaggtc
     3301 acggcccgac cggaccagac acgcccgctc cttatcgcgg cgcctgggca tcggcggctg
     3361 ccggcgtggc catggtggtg atggcgccgg tcttcatcta cctcgtcgac caccccaccg
     3421 tccccttctt cggggtgcac gacttcaa
//
LOCUS       DATMFS010000110         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_283458, whole genome shotgun sequence.
ACCESSION   DATMFS010000110 DATMFS010000000
VERSION     DATMFS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283458"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VNQ33_02045"
     CDS             <1..565
                     /locus_tag="VNQ33_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96908.1"
                     /translation="VRLALTAAGGGQYRFAGDLDLDVIDVAPLPTDELLAQLVADDAE
                     LTDLELNVVAHTWAHDGAEPMDIVAVDEHTGARLASAPGRLVATAAGIVAMKATTVPL
                     RASSKPEKRASDLYDLGRLLVAGRLTAADLHALPEPLHRSVAGRLRQWFVDGAGRDRT
                     YRDVRRFDEPLLDLDDAADAVEDLVGI"
     gene            799..1395
                     /locus_tag="VNQ33_02050"
     CDS             799..1395
                     /locus_tag="VNQ33_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ96909.1"
                     /translation="MAAPAVEILRWPAHAGRREDLVAAQRPCLWVLETGELPPAIGPL
                     EDWAREGDSERDVHARVQRLAALAPDVRALVPREAQVGEDGLLRFRGHLIAVPPSEAR
                     LLDRLAETPGTIVGRDELGRLLWDRPPASRSSLTSRIHTLRKRLRPVGLTIHAIRGHG
                     FLLTAEAPVADGPSGGRRGAVEAPSSDPSTIDPATGTR"
     gene            1405..1743
                     /locus_tag="VNQ33_02055"
     CDS             1405..1743
                     /locus_tag="VNQ33_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96910.1"
                     /translation="MRRAGREERKRIASLVLVAVIATFSLPFGASAVTQAVDATPAQR
                     AHVKRLHVEARPVTASRRPIVGRDATLPPTSILVAALTIVGLLAGTRFLHLVWMPGLI
                     PVQRGPPSLR"
     gene            1797..>2083
                     /locus_tag="VNQ33_02060"
     CDS             1797..>2083
                     /locus_tag="VNQ33_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96911.1"
                     /translation="MTTFAPSDAPIALLRWPADADRRSELSADGRPRLLLLDPGADPP
                     ASWDVLEDWVRMPADPAEIEARIATLMRRTPTERPAPLLDGDGVLRVGATWV"
BASE COUNT          257 a          838 c          713 g          275 t
ORIGIN      
        1 ggtccggctg gcgctcaccg ccgcgggcgg cggccagtac cggttcgccg gcgacctcga
       61 cctcgacgtg atcgacgtgg ccccgctgcc caccgacgag ctgctcgccc agctggtggc
      121 cgacgacgcc gagctcaccg acctcgagct gaacgtggtg gcccacacct gggcccacga
      181 cggtgccgag ccgatggaca tcgtcgccgt cgacgagcac accggcgccc ggctggcctc
      241 tgctccgggt cgcttggtcg ccactgctgc cgggatcgtc gccatgaagg cgaccaccgt
      301 gcccttgcgg gcatcctcga agccggagaa gcgggcgtcg gacctctacg acctcggccg
      361 cctgctcgtc gccgggcggc tcacggcggc tgacctccac gccctccccg aaccgctgca
      421 ccggtccgtg gccgggcgcc tccgccagtg gttcgtcgac ggtgccggcc gggaccggac
      481 ctaccgggac gtgcggcgct tcgacgagcc gctcctcgat ctcgacgacg ccgccgacgc
      541 ggtggaggac ctggtcggca tctgatcggg gcggaccgac gggggccggc tcgttcagat
      601 ccgccaccga gtcctggtgg gttgcgacag gtaggcgtca gcgaaccgcc cgatacctgt
      661 tacgtcgcgc gcacgaccgt gaaatcctgg cttcgtacgc tgcccgccat gacccccgtc
      721 cggctcccgt ggtccggcga cgcggcgggt tcgcccggcc ccgtcgagca gcccgccggg
      781 accgagtcgg tctgagccgt ggctgcgccc gccgtcgaga tcctgcgctg gcctgctcac
      841 gccggccggc gcgaggacct cgtcgcggcc cagcggccct gcctctgggt gctcgagacc
      901 ggcgagctcc cgccggccat cgggcccctg gaggactggg cccgcgaggg cgacagcgaa
      961 cgtgacgtcc acgcgcgggt ccagcgcctc gccgctttgg ccccggacgt gcgggcgctc
     1021 gtcccccgtg aggcccaggt gggcgaggac ggcctgctgc gcttccgggg ccacctcatc
     1081 gccgtcccgc cctccgaggc ccgcctgctc gaccggctgg ccgagacccc cgggaccatc
     1141 gtcggccgag acgagctggg ccgcctcctg tgggaccggc cgccagcgag ccgaagctcg
     1201 ctcaccagcc ggatccacac ccttcgcaag cggctccggc cggtggggct cacgatccac
     1261 gccatccgcg gccacgggtt cctgctgacg gcagaggccc ccgtcgccga tggtccgtcc
     1321 ggaggccggc gaggcgcggt cgaggcgcca tcgagcgacc cgtcgaccat cgacccggca
     1381 accggaaccc gataggaagt acccatgcgc agagcaggca gggaggagcg gaagcggatc
     1441 gcgagcctcg tgctcgtggc cgtcatcgcc acgttctccc tgccgttcgg cgcctcggcg
     1501 gtcacccagg cggtcgatgc cacgcccgcc cagcgggccc acgtcaagcg cctccacgtg
     1561 gaggcccggc cggtcaccgc cagccggcgg ccgatcgtcg ggcgggacgc gaccctcccg
     1621 ccgacctcga tcctcgtcgc cgccctcacc atcgtcggcc tcctggccgg cacccggttc
     1681 ctgcacctgg tgtggatgcc gggcctcatc cccgtccagc ggggaccccc ttcgctgcgc
     1741 tgaaccccgt tctccgcaga tctcgggttc gtgcccgagc gacgaaaggt gttcccatga
     1801 ccacgttcgc cccgagcgac gcgcccatcg cgctcctgcg ctggccggct gatgccgacc
     1861 gccgcagcga gctcagcgca gacggccggc cccgcctgct gctcctcgac cccggcgcgg
     1921 acccgcccgc cagctgggac gtcctcgaag actgggtccg catgccggcc gaccccgccg
     1981 agatcgaggc acgcatcgcc acgctgatgc gccgcacgcc gaccgaacgc cccgccccgc
     2041 tgctcgacgg cgacggcgtg ctgcgcgtcg gcgccacgtg ggt
//
LOCUS       DATMFS010000111         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_121174, whole genome shotgun sequence.
ACCESSION   DATMFS010000111 DATMFS010000000
VERSION     DATMFS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121174"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNQ33_02065"
     CDS             complement(<1..432)
                     /locus_tag="VNQ33_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96912.1"
                     /translation="MTALDSPSTIDRYAQQVRDACQDLPASARDELLEDLDEHLADAF
                     ADLGEGETLADRLGSAEAYAADLRQSAGYPLAAGASTARRRGRFAARVRSLWDESETH
                     PQGRAVREYLTDLRPAWWLARAYLVVTGLAEITSSDGLNVIP"
     gene            complement(429..761)
                     /locus_tag="VNQ33_02070"
     CDS             complement(429..761)
                     /locus_tag="VNQ33_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWJ96913.1"
                     /translation="MDTTQLLKGVLDLAVLAVLDGEDGYGYDVLRRLRNAGLHDVGDA
                     SVYGTLRRLFHAGKLTSYVVPSEEGPNRKYYGLNDAGRDQLHESAQTWRSFAKTLDDL
                     LDTRKAGA"
     gene            complement(867..1946)
                     /locus_tag="VNQ33_02075"
     CDS             complement(867..1946)
                     /locus_tag="VNQ33_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HWJ96914.1"
                     /translation="MADITVPEVDGVITASEVQATVDAIADWQLPNGMVPWYPGGHAD
                     PWNHVEAAMALALGGRVAEAERAYDWLVGLQRPDGSWHQYYVEDGVEDDKFDLNVVAY
                     VASGVWHHWLLTTDRGFAETMWPVVERAIDFVLELQQPRGEILWARHADGTPWPFALL
                     TGSSSICHSLRCAIALAELLGHERPDWELSAARLAHVIRHQEADAFAPKHRWAMDWYY
                     PVLGGVVLGDAGRARLAERFDTFIDDGRGVRCVKDRPWITVAETCECVLAHLAVGERE
                     TAQQLFSWIQQYRVEDGRYWTGTVYPDLSHFPADEQSTYTAAAVVLGADGLWGEGPTS
                     ALFTDHDAVLPPLIDISDEDATITD"
     gene            complement(1939..>2549)
                     /locus_tag="VNQ33_02080"
     CDS             complement(1939..>2549)
                     /locus_tag="VNQ33_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ96915.1"
                     /translation="ADLKDCSGLFQAMEEAGEAPAGTHWATVNGDGTGLPFGDATFDV
                     IICSEVMEHIPDDSAAAAELARVLKPGGLLAVTVPTFLPERICWALSDEYHAPFVPGG
                     HVRIFTEVELKNRFTTAGLVPEGTHHAHALHSPYWWLKCAVGPTNDDNPLVQAYLKLL
                     TWDIMKAPKLTRWTEKALNPVLGKSLVLYARKPAKVAEPVGG"
BASE COUNT          343 a          840 c          980 g          386 t
ORIGIN      
        1 cgggatgacg ttgaggccgt cgctggaggt gatctcggcc aggccggtga ccacgaggta
       61 ggcgcgggcg agccaccagg ccgggcggag gtcggtcagg tactcccgca cggcgcggcc
      121 ctgcgggtgg gtctcggact cgtcccagag ggagcggacc cgcgccgcga accggccccg
      181 ccgccgggcg gtcgaggcgc cagccgcgag cgggtagccg gcggactggc gcaggtcagc
      241 ggcgtacgcc tcggcgctgc cgagccggtc ggccagcgtc tcgccctcgc ccaggtcggc
      301 gaaggcgtcg gccaggtgct cgtcgaggtc ctcgagcagc tcgtctcgtg cgctcgcggg
      361 aagatcctgg caggcgtcgc gcacctgctg ggcgtagcgg tcgatggtgc tcgggctgtc
      421 gagtgcggtc atgcgccggc ctttcgggtg tcgaggaggt cgtcgagggt cttggcgaac
      481 gaacgccagg tctgggcgga ttcgtggagc tggtcgcgtc cggcgtcgtt gagcccgtag
      541 tacttccggt tggggccctc ctccgagggc accacgtagc tggtgagctt cccggcgtgg
      601 aacaggcggc gcagcgtgcc gtacacggac gcatcgccga cgtcgtgcag cccggcgttg
      661 cggagccgcc gcaagacgtc gtacccgtag ccgtcctcac cgtcgagcac ggcgaggacg
      721 gcgaggtcca gtacgccctt gagcagttgc gtggtgtcca tcccagcctc ccttcgctag
      781 tgtttctacc gcactagtac gcactgcgca ataccgtcct caccgcagta ccgccccgtc
      841 ccccgagtcg gggaggggtg ggccggtcag tcggtgatgg tggcgtcttc gtcgctgatg
      901 tcgatcagcg gtgggaggac cgcatcgtga tcggtgaaca gcgcgctggt ggggccttcg
      961 ccccacaggc cgtcggcgcc cagcacgacc gccgccgcgg tgtaggtgga ctgctcgtcg
     1021 gcggggaagt gcgacaggtc cgggtagacg gtgccggtcc agtagcggcc gtcctccacc
     1081 cggtactgct ggatccagga gaagagctgc tgggccgtct cccgctcacc gacggcgagg
     1141 tgggcgagga cgcactcgca ggtctcggcc acggtgatcc acggccggtc cttcacgcag
     1201 cggacgccgc ggccgtcgtc gatgaacgtg tcgaagcgct cggcgaggcg ggctcggccg
     1261 gcgtcgccca ggaccacgcc gccgaggacc gggtagtacc agtccatggc ccagcggtgc
     1321 ttgggggcga aggcgtcggc ctcctggtgg cggatgacgt gggcgaggcg ggcggcggac
     1381 agctcccagt cggggcgctc gtggccgagg agctcggcca gcgcgatggc gcaccgcagc
     1441 gagtggcaga tcgacgacga gcccgtgagc agggcgaagg gccacggtgt gccgtcggcg
     1501 tggcgggccc acaggatctc gccgcggggc tgctggagct cgagcacgaa gtcgatggcc
     1561 cgctcgacca ccggccacat cgtctcggcg aagccgcggt cggtcgtgag gagccagtgg
     1621 tgccacacgc ccgatgcgac gtaggcgacc acgttgaggt cgaacttgtc gtcctcgacc
     1681 ccgtcctcga cgtagtactg gtgccaggag ccgtcggggc gctggaggcc gacgagccag
     1741 tcgtaggccc gctcggcctc ggcgacgcgg ccgccgagcg cgagggccat ggccgcctcg
     1801 acgtggttcc acgggtcggc gtggccgccc gggtaccagg ggaccatgcc gttgggcagc
     1861 tgccagtcgg cgatggcgtc gacggtggcc tgcacctcgg acgcggtgat cacgccgtcg
     1921 acctcgggca cggtgatgtc agccaccgac gggctccgcg accttggccg gcttgcgggc
     1981 gtagagcacg aggctcttgc cgagcacggg gttgagcgcc ttctcggtcc accgggtcag
     2041 cttgggcgcc ttcatgatgt cccaggtcag gagcttgagg tacgcctgga ccagggggtt
     2101 gtcgtcgttg gtggggccca ccgcgcactt cagccaccag tagggcgagt gcagggcgtg
     2161 ggcgtggtgg gtcccctcgg ggaccaggcc ggccgtggtg aagcggttct tcagctccac
     2221 ctcggtgaag atccgcacgt ggccgccggg cacgaacggg gcgtggtact cgtcgctcag
     2281 cgcccagcag atccgctcgg gcaggaacgt cggcacggtg acggccagca ggccgcccgg
     2341 cttcagcacc cgagccagtt cggcggccgc cgcgctgtcg tcggggatgt gctccatcac
     2401 ctcggagcag atgatgacgt cgaacgtggc gtcaccgaac gggaggccgg tgccgtcgcc
     2461 gttgacggtg gcccagtggg tgccggctgg tgcctcaccc gcctcctcca tcgcctggaa
     2521 caggccggag cagtccttga ggtcggcga
//
LOCUS       DATMFS010000112         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_27869, whole genome shotgun sequence.
ACCESSION   DATMFS010000112 DATMFS010000000
VERSION     DATMFS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27869"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /gene="cysD"
                     /locus_tag="VNQ33_02085"
     CDS             complement(<1..649)
                     /gene="cysD"
                     /locus_tag="VNQ33_02085"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWJ96916.1"
                     /translation="MSTTYELSHLRVLEAESIHIIREVAAEFERPVLLFSGGKDSIVM
                     LRLAEKAFWPARIPFPVMHVDTGHNFPEVIEFRDRRVAELGVKLVVASVQQAIDDGRV
                     VEQKGPRASRNRLQTTALLDGIAENRFDAAFGGARRDEEKARAKERVYSFRDEFGQWD
                     PKNQRPELWSLYNGRHHQGEHLRIFPLSNWTELDIWQYIAEEQVDIPSIYYAHERE"
     gene            complement(777..1571)
                     /locus_tag="VNQ33_02090"
     CDS             complement(777..1571)
                     /locus_tag="VNQ33_02090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWJ96917.1"
                     /translation="MSPSPHDLTDDHLLAAELAEGAGAVLLEVRARLQAQGASASDLK
                     AAGDAAAHEHLMERLAQARPGDAVLSEEGTGSTGRTGTDRLGHPRVWIVDPLDGTREF
                     SEPPRTDWAVHVALVSGGQPVAGSVALPAQGRILSRVPASVLPPPPPARPRVIVSRSR
                     PPAVALHLAEAIGGDLVEMGSAGAKVAAVVLGQADVYPHSGGQYEWDSCAPVAVATAA
                     GLVATRLDGSPLVYNNADPYLPDLLVCHPHLALEVRQVLDRWLASH"
     gene            complement(1579..>2018)
                     /locus_tag="VNQ33_02095"
     CDS             complement(1579..>2018)
                     /locus_tag="VNQ33_02095"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWJ96918.1"
                     /translation="QQIDDASFCTAAYLRVELSEPFTGQVRIAASSAGHPRPVLVRAD
                     GRAEVLDCAGTLLGVIAEPVLLDVHAELGPGDAVVLYTDGVTEARRGNELFGESRLVE
                     ALGSLAGRPAEEIASALETMVADFRRSASDDTAILVVQAVAPA"
BASE COUNT          291 a          705 c          747 g          275 t
ORIGIN      
        1 cctcgcgctc gtgggcgtag tagatgctcg ggatgtccac ctgctcctcg gcgatgtact
       61 gccagatgtc gagctcggtc cagttgctca gcgggaagat gcggaggtgc tcgccctggt
      121 ggtggcggcc gttgtagagg ctccacagct ccgggcgctg gttcttcggg tcccactggc
      181 cgaactcgtc gcggaagctg tacacccgct ccttggcccg ggccttctcc tcgtcgcggc
      241 gggccccgcc gaacgccgcg tcgaagcggt tctccgcgat gccgtcgagc agtgccgtgg
      301 tctgcagccg gttgcggctc gcccgcggcc ccttctgctc gaccacacgg ccgtcgtcga
      361 tggcttgttg gaccgaggcg accaccagct tcacacccag ctcggccacc cggcggtccc
      421 ggaactcgat gacctcgggg aagttgtggc cggtgtcgac gtgcatgacc gggaacggga
      481 tgcgggccgg ccagaaggcc ttctcggcga ggcgcagcat cacgatcgag tccttgcccc
      541 cgctgaagag cagcaccggc cgctcgaact cggcggccac ctcgcggatg atgtggatcg
      601 actcggcttc gagcacccgc aggtgggaca gctcgtaggt ggtgctcatg cgctcactcg
      661 ggacgacgga cgggacgttc accaacagat cggcaggcgg cgccgccacc caagcgatcc
      721 ggggcgagcg ccaagggtag ggggccaccc ccaatcctga ccagctgact caacaatcag
      781 tgggaggcca gccaccggtc gagcacctga cggacctcca gggcgaggtg cggatggcag
      841 accagcaggt ccggaaggta gggatcggcg ttgttgtaga ccagcgggct gccgtcgagc
      901 cgggtggcca ccaagccggc ggcggtggcg acggcaaccg gcgcacacga gtcccactcg
      961 tactggccgc ccgagtgcgg gtacacgtcg gcctggccca acacgacggc cgcgaccttc
     1021 gcccccgccg atcccatctc caccaggtcg ccgccgatcg cctcggcgag gtgcagcgcc
     1081 accgccggcg gacgtgagcg gctcacgatc acccgggggc gagcaggcgg gggcggcggg
     1141 agcaccgatg ccgggacccg gctgaggatg cggccttgag ccggcagggc caccgacccc
     1201 gcgaccggtt ggccgccgga caccagggcg acgtggacgg cccagtccgt gcgcggcggc
     1261 tcggagaact cgcgggtgcc gtcgagcggg tccacgatcc agacccgcgg gtggcccagg
     1321 cggtcggtac cggtccggcc ggtcgacccg gtgccctcct cggagaggac ggcgtcgccg
     1381 ggtcgcgcct gggccaagcg ctccatcagg tgctcgtggg cggcggcgtc gccggccgcc
     1441 ttcaggtccg aggccgacgc gccctgcgcc tgcagccggg cccggacctc gaggagcacg
     1501 gcaccggctc cttcggccag ttcggcggcg aggaggtggt cgtcggtcag atcgtgcgga
     1561 gaggggctca cccctgcatc atgccggggc gacggcttgc acgacgagga tggcggtgtc
     1621 gtcgctggcg ctgcgccgga agtcggcgac catcgtctcg agcgccgatg cgatctcctc
     1681 ggcgggacgg cccgcgagcg aaccgagcgc ctcgaccaag cgggactcgc cgaacagctc
     1741 gttgccccgc cgggcctcgg tgaccccgtc ggtgtagagc acgacggcgt cgccgggacc
     1801 gagctcggcg tggacgtcga gcagcaccgg ctcggcgatg acgcccagca gcgtgccggc
     1861 gcagtcgagg acctctgcac gcccgtcggc ccggaccagg acgggacggg gatggccggc
     1921 gctggacgcg gcgatccgaa cctggccggt gaacggttcg ctgagctcga cccgcaggta
     1981 ggccgcggtg cagaagctgg cgtcgtcgat ctgctgga
//
LOCUS       DATMFS010000113         2665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_27922, whole genome shotgun sequence.
ACCESSION   DATMFS010000113 DATMFS010000000
VERSION     DATMFS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27922"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VNQ33_02100"
     CDS             complement(<1..1027)
                     /locus_tag="VNQ33_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HWJ96919.1"
                     /translation="MTAPRLEVRLDLIERNTRTLVDRLAPKGIAVHAVTKAALGSPAV
                     ARAFIAGGAAGLADSRAANGERLRAARIRSPILSIRSPMVSEADRIVRTFETSANTEH
                     DVITALSAAASAHGWSHGVLLMVELGDLREGILPRDVEVVARHALSLPSIELRGIGSN
                     LACRSGVIPDDANMAELSDLATSLERSLGVALPVVSGGNSANLTWALATGDTGRINHL
                     RLGEALLLGCEPLERRPVDGLSTDAFSLVAEVIESKVKPVQPWGELAQTAFGERPRRG
                     VGGNAIQTIVALGRQDVDAGDLTPPAGAVIVASSSDHLVLETDHLLRPGTELAFRPGY
                     AALLRAMT"
     gene            complement(1024..2187)
                     /locus_tag="VNQ33_02105"
     CDS             complement(1024..2187)
                     /locus_tag="VNQ33_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWJ96920.1"
                     /translation="MAPTSPSLLVDAPVDPDARSSAPVGASGGVTAVVYCEGNFGEID
                     GKTANGLVRHSERYRVVAVVDSRHAGADAGEVLDGRANGIPVCGSMAEALRLPSGRPD
                     RFIIGMAPASGLLSPGERATVLEAMAQGMSIVNGLHEFLDDDAEFAAAGAAYGVTIHD
                     VRRPRATKDLRMFSGRIATVDCVRIAVLGTDGAIGKRTTSTVLAQALNDAGIRTVLVG
                     TGQTSIIQGARYGIALDAIPAQFSVGELEATVVEAWEDQHPDVIIVEGQGALSHPAYL
                     SSAAVLRGSRAQAVVLQHAPARRQLSDFPGFPMPTAASEIDLIETFADTTVIGLTINH
                     EAMSDAEVSEAIVAYEADLGIPVTDALTRSPSRLVDLVLGAFPQLRRTTAAVA"
     gene            2351..>2665
                     /locus_tag="VNQ33_02110"
     CDS             2351..>2665
                     /locus_tag="VNQ33_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HWJ96921.1"
                     /translation="MADAPPPSEPTALSPLVERPTGGRTYSHPRRVRLGDVAPSGRLR
                     LDAAARYLQDVAGDDSTEVMGAEAFTWVVRRTTIEVQQFPVFDEEVTVTTWGSGTGSR
                     WAE"
BASE COUNT          366 a          955 c          981 g          363 t
ORIGIN      
        1 aggtcatggc ccggaggagg gcggcgtagc cggggcggaa cgccagctcg gtcccgggcc
       61 gcagcaggtg gtccgtctcc aagacgaggt ggtcgctcga cgaggccacg atgaccgcac
      121 ccgccggggg cgtgaggtcc ccggcgtcga cgtcttggcg gcccagggcg acgatcgtct
      181 ggatggcgtt cccgccgaca ccgcggcgcg gtcgttcgcc gaaggccgtc tgagccagct
      241 cgccccacgg ctggaccggc ttcaccttgg actcgatcac ctcggcgacc agggagaagg
      301 catccgtcga gagcccgtcg acgggtcggc gttcgagcgg ctcgcacccg aggagcagcg
      361 cctcgccgag gcggaggtgg ttgatccggc cggtgtcgcc cgtggcgagc gcccacgtca
      421 ggttggccga gttcccgccc gacacgaccg gcagggcgac gcccagcgag cgctcgaggg
      481 aggtggcgag gtccgacagc tccgccatgt tcgcgtcgtc ggggatgacg ccgctccggc
      541 aggcgaggtt cgacccgatg ccccggagct cgatgctggg caagctcagc gcgtggcggg
      601 cgaccacctc gacgtccctc ggcaagatgc cctcgcgcag gtcccccagc tcgaccatga
      661 gcaggacccc gtggctccag ccatgggccg acgccgccgc cgacagcgcg gtgatcacgt
      721 cgtgctcggt gttcgcgctg gtctcgaagg tgcgcacgat gcggtcggcc tcgctcacca
      781 tcggcgaccg gatcgagagg atcggcgagc ggatccgcgc cgctctcagg cgctccccgt
      841 tggcggcacg ggagtcggcc agccctgcgg cgccaccggc gatgaacgcc cgagccacgg
      901 cgggcgagcc cagcgccgcc ttggtcaccg cgtggacggc gatgcccttc ggcgccagcc
      961 ggtcgaccag cgtccgggtg ttgcgctcga tgaggtccag ccgcacctcg agccggggcg
     1021 cggtcatgcg acggcggcgg tcgtcctccg cagctggggg aaggcaccca ggaccaggtc
     1081 gaccagccgg ctcggcgaac gggtgagggc gtccgtgacc gggatgccga gatcggcttc
     1141 gtaggcgacg atcgcctccg agacctccgc gtcgctcatc gcctcgtggt tgatcgtgag
     1201 cccgatcacc gtggtgtcgg cgaaggtctc gatcaggtcg atctcgctgg cggccgtcgg
     1261 catggggaac ccggggaagt ccgacagctg gcggcgcgcc ggcgcgtgct ggagcacgac
     1321 ggcctgggcc cgactgcccc gcaggaccgc tgcggaggag aggtacgccg ggtggctgag
     1381 ggcgccctgg ccctcgacga tgatgacgtc ggggtgctgg tcctcccagg cctcgacgac
     1441 cgtggcctcg agctcgccca cgctgaactg cgccgggatg gcgtcgaggg cgatgccgta
     1501 ccgggcgccc tggatgatgc tcgtctggcc ggtgccgacc agcacggtgc ggatcccggc
     1561 gtcgttgagc gcctgggcca gcaccgtcga tgtcgtgcgc tttccgatgg cgccgtcggt
     1621 gccgagcacg gcgatgcgaa cgcagtcgac cgtggcgatc cgcccgctga acatgcggag
     1681 gtccttcgtg gcccggggcc ggcgcacatc gtggatcgtg accccgtacg cggcgcccgc
     1741 ggcagcgaac tcggcgtcat cgtcgaggaa ctcgtgcagg ccgttcacga tgctcatgcc
     1801 ctgcgccatg gcctcgagca ccgtggcccg ctcgcccggc gacagcaggc cgctcgccgg
     1861 tgccatgccg atgatgaacc ggtccggtcg cccggacggg agccgcagcg cctcggccat
     1921 cgacccgcac accgggatgc cgttggctcg cccgtcgaga acctcgcccg cgtcggcgcc
     1981 ggcgtggcgg ctgtcgacca cggccaccac ccggtaccgc tccgagtgcc gcacgagccc
     2041 gttggcggtc ttgccgtcga tctcgccgaa gttcccctcg cagtagacga ccgcggtcac
     2101 gccgccggac gcaccgaccg gcgcggacga acgggcatcg ggatcgacgg gggcatcgac
     2161 gagcagggaa ggggacgtgg gggccatggg ctctcctcga gtcagggctc agaggaggcg
     2221 accggaatcg tgacggccgc cttgtgcagc cgtaggtcaa cgagaggtcc ccgctaagtc
     2281 gtacaggtgt tcttcaccct agttcacctg gtcacgaacc ccaccgccgg ccgtgggcca
     2341 acatgcaccc atggccgatg ccccgccccc gtccgagccc accgccctct cgcccctcgt
     2401 cgagcggccg acgggcgggc ggacgtactc ccacccccgg cgggtccggc tgggggacgt
     2461 ggcgccgtcg ggccggctcc ggctcgacgc cgccgcccgc tacctgcagg acgtcgccgg
     2521 cgacgactcc accgaggtca tgggcgccga ggcgttcacg tgggtcgtgc gacggaccac
     2581 catcgaggtg cagcagttcc cggtgttcga cgaggaggtc accgtcacca cctggggctc
     2641 gggcaccggc agccggtggg ccgag
//
LOCUS       DATMFS010000114         5717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_306688, whole genome shotgun sequence.
ACCESSION   DATMFS010000114 DATMFS010000000
VERSION     DATMFS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306688"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1253
                     /gene="ftsY"
                     /locus_tag="VNQ33_02115"
     CDS             102..1253
                     /gene="ftsY"
                     /locus_tag="VNQ33_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWJ96922.1"
                     /translation="MEPVILILIALVIVIGAASLLVTTRKRREKVELEAVRQSTLAPG
                     APTVPTEVKEAIAKKEADEAIASAVEGEADAPPVIVAPVEAPAKRPSFRERLSKARAT
                     FAGFLGRSAIDAGTWDELEEALIRADLGVGPTTALIERLRARVEEDGLESGDELVAVL
                     KAEMKSRLAGKDRRLKVDEGQTNVWLFVGVNGVGKTTTIGKLGKRMAGEGHRVVMAAG
                     DTFRAAAAEQLGTWADRSGAQLVRGAEGGDPSAIIFDAIESAAAKGADLVLADTAGRL
                     HTKSNLMEELKKVRRVADKDPGHVTEVLLVIDATTGQNGLVQARQFTEATEVTGVVLT
                     KLDGSAKGGIVFAIQSELGIPVKLVGLGETADDLVDFDPDEFVDALFSA"
     gene            1308..1517
                     /locus_tag="VNQ33_02120"
     CDS             1308..1517
                     /locus_tag="VNQ33_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWJ96923.1"
                     /translation="MLKVLLLPFRALILFFKLSGVKGGLLFLIGIGVGLLVAPQTGPE
                     LRARIQAKLADRNGGLPAEQEYNRL"
     gene            1559..3001
                     /gene="ffh"
                     /locus_tag="VNQ33_02125"
     CDS             1559..3001
                     /gene="ffh"
                     /locus_tag="VNQ33_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWJ96924.1"
                     /translation="MFESLSDRFDGIFKNLRGKGRLSEADVDEVLREIRLALLEADVN
                     FAVVKGLVGRIRERAVGADLHAALDPAAQVIDIVHQELIAILGGETIRITYESKPPTV
                     ILMAGLQGSGKTTTSAKLARWFKQQGRNPMLVGADLQRPAAVAQLRTLAKQVDVPVHS
                     ADDDVVASGQGDPVAVAQGCIAEAKRLGKDVVIVDTAGRLAIDAELMEQVRQISAATS
                     PKYTFLVVDAMTGQDAVTVAENFHEALALDGVILTKLDGDARGGAALSVKEVVGKPIA
                     FASTGEKLDDFDLFHPDRLAGRILGMGDILTLIDKAKEAFDEQEAEAAANRMLEGQFT
                     LEDFLEQMAQVKKMGNLGNLMKMMPGMPKELRNQEIDDRELSKIEGIIHSMTIEERRD
                     PKIIDGSRKLRISRGAGVDVTTVNALLKQFGEMQKMMKGMGAGMLGRGKKAKKGKKGG
                     RTTPKGGAPKFTLPPSAIGDAKPEFKLPGL"
     gene            3138..3428
                     /gene="rpsP"
                     /locus_tag="VNQ33_02130"
     CDS             3138..3428
                     /gene="rpsP"
                     /locus_tag="VNQ33_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWJ96925.1"
                     /translation="MAVKLRLMRMGKKKQPTYRVVAADSRSPRNGRFIEIVGTYQPRQ
                     EPSVVLIDNEKALKWLRNGAQPTDTVERLLKISGAWDEFKGTKAPAAAGAES"
     gene            3425..3721
                     /locus_tag="VNQ33_02135"
     CDS             3425..3721
                     /locus_tag="VNQ33_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWJ96926.1"
                     /translation="MSDADATPEVEESAAPTEAPNAVSVLTYVVKQVVDNPDAVSVST
                     TEGRRGGLQLDVRVGDGDMGRVIGKRGRIAQSIRTVVRAAAVKDGAQVDIEFVD"
     gene            3771..4253
                     /gene="rimM"
                     /locus_tag="VNQ33_02140"
     CDS             3771..4253
                     /gene="rimM"
                     /locus_tag="VNQ33_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWJ96927.1"
                     /translation="MTQLEIGRIGKAHGLRGEVTAVITSDRPERTAPGAVWILDAGSF
                     TVVSIRPFQAKWIAALGGVETREAAEALTGSVILGDPIDDDDALWAHELLGATVVTPD
                     GQEWGTVAGVYPNPASDLIELPDGTLIPTVFVTDASGLPDRVVVDPPEGLLQGEPEEA
                     "
     gene            4264..4479
                     /locus_tag="VNQ33_02145"
     CDS             4264..4479
                     /locus_tag="VNQ33_02145"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HWJ96928.1"
                     /translation="MPLPLFSRVRLATDEHEADGAPLGTIGSIIEVYEGGAAYEIEVS
                     DADGRTLALFAAEPAALELVDPDESGR"
     gene            complement(4476..5198)
                     /gene="trmD"
                     /locus_tag="VNQ33_02150"
     CDS             complement(4476..5198)
                     /gene="trmD"
                     /locus_tag="VNQ33_02150"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWJ96929.1"
                     /translation="MRIDVFTIFPHLVEGFTSQALLGRAQDRSLLDIRAHDLRSETSD
                     PHRTVDDSPFGGGAGMVLMAEPLFAAVEAVDPPRPLFLLGPGGRTLDQAFARELAAGD
                     GFSLLCGRYEGVDARVVDHLVDGEISIGDYVLAGGEAAALVVIEAVGRLVPGVMGNTT
                     SGDDESFSEGLLEYPHYTRPAEFRGWEVPDVLRSGDHGRVARWRHAQALARTIRDRPD
                     LIEARGGLSNADRKVLAEFDLA"
     gene            5318..5665
                     /gene="rplS"
                     /locus_tag="VNQ33_02155"
     CDS             5318..5665
                     /gene="rplS"
                     /locus_tag="VNQ33_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWJ96930.1"
                     /translation="MKTTDVIDQASLRTDVPDFQPGDTLKVHVRVVEGNKQRVQVFQG
                     VVIRRQNGGIHETFTVRKLSYGVGVERTFPVHSPIIEKIEVFTRGDVRRAKLYYLRDR
                     VGKAAKVKEKRDF"
BASE COUNT          913 a         2116 c         1900 g          788 t
ORIGIN      
        1 acgctcggaa cggcccccgc caactgatcg gcacccgccc ccgaccgtga cggccccggc
       61 tccgagaggg gccggggtcc accggtagga tccgtgccgt catggagccc gtcatcctca
      121 tcctcatcgc cctggtcatc gtgatcgggg ctgccagcct cctcgtcacc acgcgcaagc
      181 ggcgcgagaa ggtcgagctc gaagctgtcc gccagtccac cctggccccc ggagcgccca
      241 ccgttcccac cgaggtcaag gaggccatcg ccaagaagga ggccgacgag gccatcgcct
      301 ccgcggtcga aggcgaggcc gacgcaccgc cggtgatcgt cgcgcccgtc gaggctcccg
      361 ccaagcggcc gagcttccgc gagcgcctgt cgaaggcccg ggccacgttc gccgggttcc
      421 tcggccgctc ggccatcgac gccggtacct gggacgagct ggaggaggcg ctgatccgcg
      481 ccgacctcgg cgtcgggccg accacggcgc tcatcgagcg cctccgcgcc cgggtcgaag
      541 aggacgggct ggaatccggc gatgagctgg tcgcggtgct caaggccgag atgaagtccc
      601 gcctcgccgg caaggaccgc cgcctgaagg tcgatgaggg ccagaccaac gtgtggctgt
      661 tcgtcggcgt caacggcgtc ggcaagacca ccaccatcgg caagctcggc aagcgcatgg
      721 ccggcgaggg ccaccgcgtc gtcatggccg ccggcgacac cttccgcgcc gctgccgcgg
      781 agcagctggg cacgtgggcc gaccgatcgg gcgcccagct cgtgcgcggc gccgagggcg
      841 gcgatccgtc cgccatcatc ttcgacgcca tcgagtccgc cgccgccaag ggcgcggacc
      901 tggtcctggc cgacaccgct gggcgcctcc acaccaagag caacctcatg gaggagctca
      961 agaaggtccg tcgggtggcg gacaaggacc cgggccacgt caccgaggtc ctgctcgtga
     1021 tcgacgccac caccggccag aacggcctcg tgcaggcccg ccagttcacc gaggccaccg
     1081 aggtcaccgg tgtggtcctc accaagctcg acggctccgc caagggcggc atcgtcttcg
     1141 ccatccagtc tgagctcggc atcccggtca agctggtggg gctgggcgag accgccgacg
     1201 acctggtcga cttcgacccc gacgagttcg tcgacgccct gttctccgct tgacccgtct
     1261 gtccaccgtt tcccgagcga cccgctcgac ccatcctgga ggttcccgtg ctgaaggtcc
     1321 tgctgctccc gttccgagcg ctgatcttgt tcttcaagct ctccggggtg aagggcgggc
     1381 tgctgttcct gatcggcatc ggggtgggcc tgctcgtcgc cccgcagacc ggcccggagc
     1441 tgcgggcacg gatccaggcc aagctggccg accgcaacgg cggcctcccg gccgaacagg
     1501 agtacaaccg cctctagttc gcggccccgt cgaggccgcc cggtagagtc gcaagcccat
     1561 gttcgagtca ctgtccgacc gtttcgacgg catcttcaag aacctccgcg gcaagggccg
     1621 cctgagcgag gccgatgtcg acgaggtgct gcgcgagatc cgtctcgccc tcctcgaagc
     1681 cgacgtcaac ttcgctgtcg tcaagggcct cgtcggccgg atccgcgagc gggcggtcgg
     1741 tgccgacctg cacgccgcgc tcgacccggc cgcccaggtc atcgacatcg tccaccagga
     1801 gctcatcgcc atcctcggcg gcgagaccat ccgcatcacc tacgagtcca agccgcccac
     1861 cgtcatcctc atggcgggcc tccagggctc tggcaagacc accacgtcgg ccaagctcgc
     1921 ccgctggttc aagcagcagg gccgcaaccc gatgctcgtc ggcgccgacc tccagcgtcc
     1981 cgccgccgtc gcgcagctgc gcacgctcgc caagcaggtc gacgtgccgg tgcactcggc
     2041 tgacgacgac gtcgtggcct ccggccaggg cgaccccgtc gccgtggccc agggctgcat
     2101 cgccgaggcc aagcgcctcg gcaaggacgt ggtcatcgtc gacaccgccg gccggctggc
     2161 catcgacgcc gagctcatgg agcaggtccg ccagatctcg gcggccacca gcccgaagta
     2221 cacgttcctc gtcgtcgacg ccatgaccgg ccaagacgcc gtcacggtcg ccgagaactt
     2281 ccacgaggcc ctcgcgctcg acggcgtcat cctcaccaag ctcgacggcg atgcccgcgg
     2341 cggtgccgcc ctctcggtga aggaggtcgt gggcaagccc atcgccttcg ccagcaccgg
     2401 cgagaagctc gacgacttcg acctgttcca cccggaccgc ctcgccgggc gcatcctcgg
     2461 catgggcgac atcctcacgc tcatcgacaa ggccaaggag gccttcgacg agcaggaggc
     2521 cgaggccgcg gccaaccgca tgctcgaggg ccagttcacg ctggaggact tcctcgagca
     2581 gatggcccag gtcaagaaga tgggcaacct cggcaacctc atgaagatga tgccggggat
     2641 gcccaaggag ctccggaacc aggagatcga cgaccgcgag ctgtccaaga tcgaggggat
     2701 catccactcg atgaccatcg aggagcggcg ggacccgaag atcatcgacg gctcccgcaa
     2761 gctgcgcatc tcccggggtg cgggcgtcga cgtcaccacc gtcaacgccc tgctgaagca
     2821 gttcggcgag atgcagaaga tgatgaaggg catgggcgcc ggcatgctcg gccggggcaa
     2881 gaaggcgaag aagggcaaga agggcgggcg caccaccccg aagggcggcg ccccgaagtt
     2941 caccctcccg ccctcggcca tcggcgacgc caagcccgag ttcaagctcc ccgggctcta
     3001 gccccgcggt ggctgccggg cctgcgcttg cgtggaccgg gctccagcga ccaccattga
     3061 tcctcggcct gagtccgctc ggccccgtcc tcacccgtat ctcccaccca gtaccagtaa
     3121 caggaagaag accactcgtg gccgtcaagc tccgcctcat gcggatgggc aagaagaagc
     3181 agcccaccta ccgcgtcgtc gccgccgact cgcggtcgcc ccgcaacggg cgcttcatcg
     3241 agatcgtcgg cacctaccag ccccgccagg agccgtcggt cgtgctgatc gacaacgaga
     3301 aggccctcaa gtggctccgc aacggcgccc agcccaccga caccgtcgag cgcctgctga
     3361 agatctccgg tgcctgggac gagttcaagg gcaccaaggc gccggccgcg gccggagccg
     3421 agtcgtgagc gacgcggacg ccacccccga ggtcgaggag tcggcggcgc ccaccgaggc
     3481 gcccaacgcc gtctccgtcc tgacctacgt ggtcaagcag gtcgtcgaca accccgatgc
     3541 cgtctcggtg tccaccaccg agggccgccg tggcggcctt cagctcgacg tccgcgtcgg
     3601 cgacggggac atgggccggg tcatcggcaa gcgcgggcgc atcgcccagt cgatccgcac
     3661 cgtcgtccgg gccgccgccg tcaaagacgg cgcccaggtc gacatcgagt tcgtcgactg
     3721 accttcggtc tgcccggcgc ctcagcgcct gccaccgaac caacccttcg atgacccagc
     3781 tggagatcgg ccgcatcggc aaggcccacg gcctgcgcgg cgaggtcacc gccgtcatca
     3841 cctcggaccg ccccgagcgc accgcgcccg gggcggtgtg gatcctggac gccggttcct
     3901 tcaccgtcgt gtcgatccgc ccgttccagg cgaagtggat cgccgcgctc ggcggcgtcg
     3961 agacccgcga ggcggccgag gcgctcaccg gctcggtgat cctcggcgac cccatcgacg
     4021 atgacgatgc gctgtgggcc cacgagctgc tcggcgccac cgtcgtcacg cccgacggcc
     4081 aggagtgggg gaccgtcgcc ggcgtctacc cgaacccggc cagcgacctg atcgagctgc
     4141 ccgacggcac gctcatcccc accgtcttcg tcaccgacgc ctccggtctc ccggaccgcg
     4201 tcgtggtcga cccgcccgag ggcctcctcc agggcgaacc ggaagaggcc tgaggctccg
     4261 gctgtgccgc ttccgctgtt ctcgcgggtc cgcttggcga ccgacgagca cgaggccgac
     4321 ggtgcaccgc tcggcacgat cgggtcgatc atcgaggtgt acgaaggcgg tgctgcctac
     4381 gagatcgagg tgagcgacgc ggatgggcgc accctcgccc tgttcgcggc ggaaccggcc
     4441 gccttggagc tcgtcgaccc cgacgagtcc ggccgctagg cgaggtcgaa ctcggcgagg
     4501 accttccggt cggcgttgct gagcccgccc cgagcctcga tcaggtcggg gcggtcgcgg
     4561 atggtgcggg ccagggcctg agcgtggcgc cagcgggcga cgcggccgtg atcgcccgag
     4621 cgcagtacat cggggacctc ccagccgcgg aactcggccg gccgggtgta gtgcgggtac
     4681 tcgagcaggc cctcgctgaa ggactcgtcg tcgccggagg tggtgttgcc catgaccccg
     4741 ggcacgagcc gcccgaccgc ctcgatcacc accagcgccg ccgcctcgcc gccggccagg
     4801 acgtagtcgc cgatcgagat ctcgccgtcg acgaggtggt cgacgacccg ggcgtcgacg
     4861 ccctcgtagc ggccgcagag caggctgaac ccgtcgccgg ccgcgagctc ccgggcgaag
     4921 gcctggtcga gcgtccgccc acccggtccc agcaagaaga gggggcgggg cggatcgacg
     4981 gcctcgaccg cggcgaacag cggctcggcc atgaggacca tgccggcgcc gccgccgaag
     5041 ggcgagtcgt cgacggtgcg gtgcggatcg gacgtctccg agcgcaggtc gtgcgcccgg
     5101 atgtccagga ggctcctgtc ctgcgccctc ccgagcagcg cctgggaggt gaagccctcc
     5161 accaggtgcg ggaagatggt gaagacgtcg atgcgcacgt cgtcagcgtg gcacagccac
     5221 tccggttggc ctcgaaccag gcgcggcgcc taacgttgcc ctctgctgtg agcgcccttc
     5281 ggtgctcccc ccaatcttcg ctcaggatca gagtcccatg aagaccaccg atgtcatcga
     5341 ccaagccagc ctccgcaccg acgtgccgga cttccagccg ggagacacgc tgaaggtcca
     5401 cgtccgcgtg gtcgagggca acaagcagcg cgtgcaggtc ttccagggcg tcgtgatccg
     5461 ccgccagaac ggtgggatcc acgagacctt caccgttcgc aagctctcct acggcgtcgg
     5521 cgtcgagcgc accttcccgg tccactcgcc gatcatcgag aagatcgagg tcttcacccg
     5581 tggcgacgtg cgccgggcga agctctacta cctgcgcgac cgcgtcggga aggccgccaa
     5641 ggtcaaggag aagcgcgact tctagtcgtg ctggacgggc cccgatcccg tcttggtgtt
     5701 cgccctcgtt gtcactg
//
LOCUS       DATMFS010000115         3637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_97520, whole genome shotgun sequence.
ACCESSION   DATMFS010000115 DATMFS010000000
VERSION     DATMFS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97520"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VNQ33_02160"
     CDS             complement(<1..439)
                     /locus_tag="VNQ33_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWJ96931.1"
                     /translation="MPRTRKPVPVHNLKVTRKGKVKKRTFLWRMRRVFYLAALAMAVL
                     VAGGIYVLGRVELPEDPTVAPVEAQTSFICSSEVQVNCNASNAMASLHGTEDRVLVTY
                     DEIPDVLREAVISAEDKDFFKHGGVDPMGITRAAYRDLKGEGVR"
     gene            complement(553..963)
                     /locus_tag="VNQ33_02165"
     CDS             complement(553..963)
                     /locus_tag="VNQ33_02165"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HWJ96932.1"
                     /translation="MAFRPDAVRGSEGPGEIDYRLARQSVLSEFKKGRIAKHEICDAH
                     PELVRAARELGEPTKLTCPVCEETNVVLVTYVFGPRLPAFGRCISSKKELQAIAKRSG
                     SFSAYVIEVCPACSWNHLARTFLLSPARAKAAAN"
     gene            complement(1048..1749)
                     /locus_tag="VNQ33_02170"
     CDS             complement(1048..1749)
                     /locus_tag="VNQ33_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWJ96933.1"
                     /translation="MRIVSFNIHHGTVGRRGPVDPEQLGAVCASFDADVIALQEVDRD
                     TYRTARADLAAVAAGACGMAHVFGSSRWYPGGRYGNALLARGPITAHTVTKLPKVPEG
                     QFWQEQRTALEASVTIDGRSLWVAATHLSVPTAQNEVQLDWLLRHLKGRPRPQVVVGD
                     LNRPLSLVRTDAEQGGLRAVEHGPTFPVTKPKRAIDHVLVTPELTVLRAEVRRTPMSD
                     HAALVIDLDWVEPPG"
     gene            1832..2587
                     /locus_tag="VNQ33_02175"
     CDS             1832..2587
                     /locus_tag="VNQ33_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ96934.1"
                     /translation="MADALFPTAVPADPSWHRYSAASPTTGDTGSVVVYGTELPTETD
                     LKLLGTLEGARILDLGCGTGRNAVALAQAGAKVLAVDPSADLLAAARERAEAAEVRVE
                     LHQAELAELAFLRSDSIDAAISVMALNSSDDLARVFRQVHRVLKPEAPLVCSVPHPAF
                     AMLDPTASDPLRIARPYDDTTSRTWELGDRTVTDHPRTFADLFTTFQRSNFRIDQVIE
                     PTASSSPSRDATYADVMRTVPATLLLRARKQGN"
     gene            complement(2838..>3637)
                     /locus_tag="VNQ33_02180"
     CDS             complement(2838..>3637)
                     /locus_tag="VNQ33_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ96935.1"
                     /translation="SRGVFHDVWVIARRGLIHMKRQPEALSDATIQPIMFVLLFAFVF
                     GGAIKVPGGGSYKEFLMGGIFAQTIVFSAFGVAMSIANDRKNQAVDRFRSLPIANGAV
                     LGGHAVANLLKAFLPIVLMSLTGLVVGWRIHSSAVDAAHGYFLMVAFSFAMIWIGVLL
                     GSLVGSPEGVTGIAFAVLFPITFIASTFVPVVTMPEPLRTIAQWNPTTSLSDALRELF
                     GNPNTPALPSDPFPIQHPVLYTWIWIVGIVVVVAPLAVRVYRKSTVD"
BASE COUNT          574 a         1310 c         1266 g          487 t
ORIGIN      
        1 gccggacgcc ctcgcccttc aggtcccggt aggcggcccg ggtgatgccc atgggatcga
       61 cgccgccgtg cttgaagaag tccttgtcct ccgcggagat cacggcctcg cggagcacgt
      121 cggggatctc gtcgtaggtg acgagcacgc ggtcctcggt gccgtgcagc gacgccatgg
      181 cgttggaggc gttgcagttg acctggacct cgctcgagca gatgaaggag gtctgggctt
      241 cgacgggggc caccgtcgga tcctccggca gctcgacgcg tcccaagacg tagatcccgc
      301 cggcgacgag cacggccatc gccagcgccg ccaggtagaa gacccgtcgc atccgccaca
      361 ggaaggtgcg cttcttgacc ttgcccttgc gcgtcacctt caggttgtgg acggggacgg
      421 gcttgcgggt gcggggcatc aggcgaaggg ggcagtcggt gccatcgacg cgaggtgcgg
      481 acggcggaaa cggcgacgag ccaggctaac ccccgaccca cgggtttggc agtcgggaca
      541 cggtccgacc ggtcagttgg cggcggcctt ggcccgagcc gggctcagca ggaacgtgcg
      601 ggccaggtgg ttccacgagc acgccgggca cacctcgatc acgtacgccg agaacgaacc
      661 agagcgcttg gcgatggcct gcagctcctt cttcgacgag atgcaccgac cgaacgcggg
      721 cagccgcggc ccgaacacgt aggtgacgag gacgacgttg gtctcctcgc acaccgggca
      781 cgtgagcttg gtgggctcgc ccagctcccg cgccgcccgc accagctccg ggtgggcgtc
      841 gcagatctcg tgcttggcga tccggccctt cttgaactcc gacagcaccg actggcgggc
      901 caggcggtag tcgatctcgc ccggtccctc ggagccgcgc acggcgtcgg gtcggaatgc
      961 catgggcgga ggctaccgat gatcggccag cttggtgcgg ggaaaccgag cccgcacacc
     1021 accggtcgac ggcgctcccg acccgggtca acccggaggt tccacccagt ccagatcgat
     1081 gaccagcgcg gcgtggtcgc tcatcggggt gcgccgcacc tcggcgcgga gcacggtgag
     1141 ctcgggtgtc accaagacgt ggtcgatggc ccgcttcggc ttggtcaccg ggaaggtcgg
     1201 cccgtgctcg accgcacgga ggccgccctg ctcggcgtcg gtgcgcacga gcgacagcgg
     1261 ccggttcaga tcgccgacga ccacctgggg gcggggccgg cccttcaggt ggcgcagcag
     1321 ccagtcgagc tggacctcgt tctgcgccgt aggcaccgac aggtgcgtgg ccgccaccca
     1381 gaggctccgt ccgtcgatgg tcaccgacgc ctccagcgcc gtccgctgct cctgccagaa
     1441 ctggccctcg ggcaccttcg ggagcttggt gacggtgtgg gcggtgatcg gcccgcgcgc
     1501 caggagggcg ttgccgtagc gaccgcccgg gtaccagcgc gagctcccga acacgtgggc
     1561 catcccgcac gcaccggccg ccacagcagc caggtcggcc cgggccgtcc ggtacgtgtc
     1621 gcggtccacc tcctggagcg cgatcacgtc cgcatcgaac gacgcgcaca cggcgccgag
     1681 ctgctcgggg tccacgggcc cccggcgccc gaccgtcccg tggtggatgt tgaagctcac
     1741 gatccgcacg acccgagcct cccacgccgg gccgcggtgc ggtaccggtg aggtacgcga
     1801 ggggcccgcg ccggggtgtg agactcgggg catggccgat gccctcttcc ccaccgctgt
     1861 ccccgccgat ccgagctggc accgctactc cgccgccagc cccaccaccg gcgacaccgg
     1921 gtcggtggtc gtgtacggca ccgagctgcc caccgagacc gatctcaagc tgctcggcac
     1981 cctcgagggg gcgcgcatct tggacctcgg ctgcggcacc ggccgcaacg ccgtggccct
     2041 cgcccaggcg ggcgccaagg tcctcgccgt cgacccctcc gccgacctgc tggccgccgc
     2101 acgagagcga gccgaggccg ccgaggtccg cgtcgagctg caccaggccg agctagccga
     2161 gctggcgttc ctccgctcgg actcgatcga cgccgccatc agcgtgatgg ccctcaacag
     2221 cagcgacgac ctcgcccgcg tcttccgcca ggtgcaccgc gtcctcaagc ccgaggcgcc
     2281 gctcgtgtgc tcggtgccgc acccggcgtt cgccatgctc gacccgacgg cgtcggaccc
     2341 cctgcgcatc gcccggccct acgacgacac caccagccgc acctgggagc ttggcgaccg
     2401 caccgtcacc gaccacccgc gcacgttcgc cgacctgttc accaccttcc agcgcagcaa
     2461 cttccgtatc gaccaggtga tcgagcccac ggcatcgagc tcccccagcc gcgacgcgac
     2521 ctacgcggat gtcatgcgca ccgtccccgc caccctcctc ttgagggcaa ggaagcaggg
     2581 caactgacga gcgggcgacg agcagcccgt cagactggcc cgcgctcgca gcggttggcg
     2641 ctggtgaccc gccagatccc atcgccgtcc cgaccggctc gcgggtcagg acccacgcgc
     2701 cccgccgcgc cgatcaccac gatgacggcg cccacggcgg aagcgacctt cgctcctcga
     2761 cccatcgatc gccgccctcc tgccgtcgcc ggcctccatc ggcacgaagg ccgccgacgg
     2821 agcgcaacgg taccgggtca gtcgaccgtg gacttgcggt acacccgcac ggccagggga
     2881 gcgacgacga cgacgatgcc gacgatccag atccacgtgt agaggacggg gtgctggatc
     2941 gggaacgggt ccgacgggag cgccggcgtg ttggggttgc cgaagagctc ccgcagcgca
     3001 tcgctcagcg aggtggtcgg gttccactgg gcgatggtgc gcagcggctc gggcatcgtc
     3061 accacgggca cgaacgtcga ggcgatgaac gtgatgggga acagcacggc gaaggcgatg
     3121 ccggtcacgc cctcaggcga gccgacgagg ctgccgagca ggacgccgat ccagatcatg
     3181 gcgaacgaga aggcgaccat caagaagtag ccgtgcgcgg catcgaccgc gctgctgtgg
     3241 atgcgccagc cgaccaccag gccggtgagc gacatgagca cgatgggcag gaacgccttg
     3301 agcaggttgg ccacggcgtg gccgccgagg acggcgccgt tggcgatggg cagcgagcgg
     3361 aagcggtcga ccgcctggtt cttgcggtcg ttggcgatgc tcatggccac gccgaaggcg
     3421 gagaacacga tggtctgggc gaagatgccg cccatcagga actccttgta ggagccgccg
     3481 cccggcacct tgatggcgcc gccgaacacg aacgcgaaca gcagcacgaa catgatcggc
     3541 tggatggtgg cgtcgctcag cgcctcgggc tggcgcttca tgtggatgag gccgcgacgg
     3601 gcgatgaccc acacgtcgtg gaacacgccg cgcgacg
//
LOCUS       DATMFS010000116         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_74368, whole genome shotgun sequence.
ACCESSION   DATMFS010000116 DATMFS010000000
VERSION     DATMFS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74368"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1187
                     /gene="serC"
                     /locus_tag="VNQ33_02185"
     CDS             51..1187
                     /gene="serC"
                     /locus_tag="VNQ33_02185"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWJ96936.1"
                     /translation="MAATPSITIPADLLPADGRFGCGPSKVRPEALEALVADAPAYLG
                     TSHRQAPVKFMVSRLRNAVAELFALPDGYEVILGNGGTTVFWDAAVFGLVEQRSQHLT
                     FGEFSSKFAECVSRAPFLGEPTVISAPPGEAAAAEAETGVDLYALTHNETSTGVAMPI
                     VRPAGADEGALVAVDATSAAGGLKFDAAQTDVYYFAPQKCLASDGGLWIAAVSPAAQE
                     RIDRIASSDRWIPASIDLKIAKDNSLKDQTYNTPALATIHLAVSQMEWINTNGGLGMS
                     SARSAKSAEIMYGWAEASDYATPFVADPAYRSNVVATIDLDDSVEATTVSAVLRANGI
                     VDTDSYRKLGRNQLRVAMFPAIDSEDIAALTTCIDYVVNELRSA"
     gene            1277..1885
                     /locus_tag="VNQ33_02190"
     CDS             1277..1885
                     /locus_tag="VNQ33_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWJ96937.1"
                     /translation="MLQTRRIPRLLAAVVGALGGAVLVAAPASAHVGHGANGFDSGFL
                     HPLTGPDHLLAMVAVGVVAATWRSDRTLWLAPAAFLLGMVGGGIAGIVGVPFPGAELL
                     IVASVILLGVAIAAAVTDGKGSAWVLPILATAGVAHGHAHGAEAPSAAHPALYIAGFL
                     LATACVHVAGVGVGTVVRDRQLVRVGLGVATATAGVLLLASA"
     gene            complement(2118..>3310)
                     /locus_tag="VNQ33_02195"
     CDS             complement(2118..>3310)
                     /locus_tag="VNQ33_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWJ96938.1"
                     /translation="EHASNLFALAEIGNIYTRIMNPTQGVVEATIAALEGATETAVGL
                     PGALAVASGQAAETLAILNLAEAGSHVVASASLYGGTYNLFHYTFPKIGIEVTFIDDP
                     DDLDAWRAAVKPNTKAFYGESIGNPKNDFLDIAAISAIALEVGVPLIVDNTVASPWLI
                     RPFEHGADIVVHSATKFIGGHGTSIGGVIVDGGSFDFSANDKFSGFTEPDPSYHGLSY
                     WPALGAGSYIIKARVQLLRDIGAAISPFNSFLLIQGLETLSLRLERHNQNAQQVAEFL
                     AQHPQVERVQYAGLPDSPWHERAKAITGGRGFGSVPAFVIAGGREAGAAFVEGLTLHS
                     HVANIGDVRSLAIHPASTTHSQLTAEEQVTAGVDPGLVRLSVGLESIEDIIADLERGF
                     AAAT"
BASE COUNT          453 a         1211 c         1161 g          485 t
ORIGIN      
        1 tccggcgaac ggcttcgacc aggggccggg cgggctgcga gactcggccc atggctgcga
       61 ccccctcgat caccatccct gccgacctcc tccccgccga cggccgcttc ggctgtggcc
      121 cgtccaaggt ccgcccggag gcgctcgagg cgctggtcgc cgacgccccc gcctacctcg
      181 gcacctccca ccgccaggca ccggtgaagt tcatggtgag ccgcctgcgc aacgccgtcg
      241 ccgagctgtt cgccctgccc gacgggtacg aggtcatctt gggcaacggc ggcaccaccg
      301 tgttctggga cgccgccgtg ttcggcctgg tcgagcagcg gagccagcac ctcaccttcg
      361 gcgagttctc gtcgaagttc gccgagtgcg tgagccgggc cccgttcctg ggcgagccca
      421 cggtgatcag cgccccgcct ggcgaggccg ccgccgccga ggccgagacc ggcgtcgacc
      481 tgtacgccct cacccacaac gagacctcga ccggcgtcgc catgcccatc gtccgccccg
      541 cgggtgccga cgagggcgcc ctcgtggccg tcgacgccac gtcggcggcc ggtggcctga
      601 agttcgatgc cgcgcagacc gacgtctact acttcgcccc gcagaagtgc ctcgcctctg
      661 atggcggcct ctggatcgcg gcggtctcgc cggccgccca ggagcgcatc gaccgcatcg
      721 ccagcagcga ccgctggatc ccggcgtcga tcgacctgaa gatcgccaag gacaactcgc
      781 tcaaggacca gacgtacaac acgccggcac tggccaccat ccacctcgcg gtgagccaga
      841 tggagtggat caacaccaac ggcgggctcg ggatgtcgtc ggcccgctcg gccaagagcg
      901 ccgagatcat gtacggctgg gccgaagcct cggactacgc gacccccttc gtggccgacc
      961 ccgcctaccg ctcgaacgtc gtcgccacca tcgacctcga cgactccgtc gaggccacca
     1021 ccgtctcggc cgtgctccgg gccaacggca tcgtcgacac cgacagctac cgcaagctcg
     1081 gccgcaacca gctgcgcgtc gccatgttcc cggcgatcga cagcgaggac atcgcagccc
     1141 tcacgacgtg catcgactac gtcgtgaacg agctccgctc cgcctgatcc gggtggcggc
     1201 ccgcccggcc ccatcgttca cgcggacgca acgttcgagc ggcgacggcg ccaaaccgct
     1261 ccggtagggt ccggccatgc tccaaacgcg ccgcatcccc cgtctgctcg ctgccgtcgt
     1321 cggtgccctc ggaggcgccg tcctcgtcgc cgcacccgct tcggcgcacg tcggccacgg
     1381 cgccaacggg ttcgactccg gcttcctcca tccgctgacg ggccctgatc acctgctggc
     1441 gatggtcgcg gtcggcgtgg tcgccgccac ctggcgcagc gaccgcaccc tctggctcgc
     1501 gccggcagcg ttcctgctcg gcatggtcgg cggcggcatc gccgggatcg tgggcgtgcc
     1561 cttccccggt gccgagctgc tcatcgtcgc gtcggtgatc ctgctcggcg tcgccatcgc
     1621 cgccgccgtc accgacggca agggctccgc ctgggtgctg ccgatcctcg ccaccgccgg
     1681 tgtcgcccac ggccacgccc acggcgccga agcgcccagc gccgcccacc cggcgctcta
     1741 catcgccggg ttcctgctgg ccacggcctg cgtgcacgtg gccggggtcg gcgtcggcac
     1801 ggtggtccgc gaccgccagc tggtgcgggt cgggctcggc gtggccaccg ccaccgccgg
     1861 ggtcttgctg ctggcctccg cctgagcgca cctcccgctc gctgagcggt ggcgcggcca
     1921 cgttcagcgc gctccgagcc ggtgaccagg ggcgggatgc cgccgcgcca gggccaaacg
     1981 acggatccgc cgcatgcatc gtccgggggg aggggtggac gatactgcga cggatccgaa
     2041 gtccggcgga ccggaccagg ccccaaccct accggatcgg gtgcgatccg ttcatcctcg
     2101 accgagcgcc gcgagcgtca cgtggccgcg gcgaagcccc gctcgaggtc cgcgatgatg
     2161 tcctcgatcg actccaggcc gaccgacagc cgcacgaggc ccgggtcgac gccggcggtc
     2221 acctgctcct cggcggtgag ctggctgtgg gtggtcgacg ccgggtggat cgcgaggctg
     2281 cggacgtcgc cgatgttggc cacgtggctg tggagcgtga gtccctcgac gaacgcggcg
     2341 ccggcctcgc ggccgcccgc gatgacgaac gccgggaccg acccgaagcc ccggccgccg
     2401 gtgatggcct tggcccgctc gtgccacggc gagtccggca ggccggcgta ctggacgcgc
     2461 tcgacctggg ggtgctgcgc caggaactcg gccacctgct gggcgttctg gttgtggcgc
     2521 tcgaggcgca ggctcagcgt ctcgaggccc tggatgagca ggaacgagtt gaacgggctg
     2581 atggccgcgc cgatgtcccg caggagctgc acccgcgcct tgatgatgta cgagccggcg
     2641 ccgagggcgg gccagtacga caggccgtgg tagctggggt cgggctcggt gaagcccgag
     2701 aacttgtcgt tggcggagaa gtcgaaggag ccgccgtcga cgatcacgcc gccgatggac
     2761 gtgccgtggc caccgatgaa cttggtggcg gagtgcacga cgatgtcggc gccgtgctcg
     2821 aacgggcgga tgagccacgg cgaggcgacg gtgttgtcga cgatgagcgg cacgccgacc
     2881 tcgagggcga tcgccgagat ggcggcgatg tcgaggaagt cgttcttcgg gttgccgatg
     2941 gactcgccgt agaacgcctt cgtgttcggc ttgaccgccg cacgccaggc gtcgaggtca
     3001 tcggggtcgt cgatgaacgt gacctcgatg ccgatcttcg ggaaggtgta gtggaagagg
     3061 ttgtaggtgc cgccgtagag cgatgcgctg gcgacgacgt ggctgccggc ctcggccagg
     3121 ttgaggatgg cgagggtctc ggccgcctgg cccgatgcca cggccagggc accgggaagg
     3181 cccacggcgg tctcggtcgc gccctcgagc gcggcgatcg tggcctcgac cacgccctgg
     3241 gtcgggttca tgatccgggt gtagatgttg ccgatctcgg ccagggcgaa caggttcgac
     3301 gcgtgctccg
//
LOCUS       DATMFS010000117         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_259280, whole genome shotgun sequence.
ACCESSION   DATMFS010000117 DATMFS010000000
VERSION     DATMFS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259280"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VNQ33_02200"
     CDS             <1..605
                     /locus_tag="VNQ33_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ96939.1"
                     /translation="DEERTRIARDLHDRIGQSLAYLAIELDRIVAVDGRGEPVGVALD
                     ELRHDVRAVIGEVRDTLYDLRTDVTDSKDMPATLQEFLDRVSGRSSLETRLDLRVTGR
                     LPLLQERELWRIAQEAVTNVERHAKATKVVVTWQSDGARALLLVADDGIGFPTSRAGR
                     LDSYGIVGMRERAASIGATLDITGTPDVGTTVRCEVRPIA"
     gene            662..1312
                     /locus_tag="VNQ33_02205"
     CDS             662..1312
                     /locus_tag="VNQ33_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ96940.1"
                     /translation="MAIRLMLADDHRMLREGLRRSLTDEGFDVIGEASDGEEAVRLAG
                     SLHPDVVLMDVTMPDVDGVEATRRIHQQHPEIRVVMLTMHADQSVIAEALRAGASGYL
                     VKDCSTDEIADAVRMAANDEATLSPEFATAMLDEVRRIDPNPTDDDADRVVTKREVEV
                     LQLIADGCSTPEVAERLFISQKTVKNHLASIYHKLDARDRTQAVLQAVRMGIVRLS"
     gene            complement(1449..1652)
                     /locus_tag="VNQ33_02210"
     CDS             complement(1449..1652)
                     /locus_tag="VNQ33_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWJ96941.1"
                     /translation="MRHQHPTRRRHKDNERGAGLIEYALLLALIAIVAFSAVSFFGTS
                     NGGGFGKSADCIKKAYEADTLCT"
     gene            complement(1742..>2575)
                     /locus_tag="VNQ33_02215"
     CDS             complement(1742..>2575)
                     /locus_tag="VNQ33_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96942.1"
                     /translation="PTVELRVLGWAALGTALGALVSPIGWDLITFPAKLLQHSDAFAN
                     TAEWESPSLSASATWMYLFQLILAVGLVAWRGRRWRAVLPVLVFGALSLQASRNIAQA
                     SLVLIIPMTLAATGIGDVVGDTPKRLLRPVQGALLALFVLIAVVGVTRQPDTDLATYP
                     EASVTWMRKEGMLSPGSRVVTRDFVGNYLEARYGPDRVVFFDDRVDMYPMSVVDQYME
                     LIDANTKPGPVLDDLDASAVLWDADSPFGDWLEDPANGWRIVRRDDDWMVAVPSGTPN
                     G"
BASE COUNT          418 a          904 c          887 g          366 t
ORIGIN      
        1 ccgacgagga gcgcacccgc atcgcccggg acctccacga ccgcatcggg cagtccctgg
       61 cctacctggc gatcgagctg gaccgcatcg tggccgtcga cgggcggggc gagccggtgg
      121 gcgtcgcgct cgacgagctg cgccacgacg tgcgcgccgt catcggcgag gtccgcgaca
      181 ccctctacga cctccgcacc gacgtcaccg actccaagga catgccggcg acgctgcagg
      241 agttcctcga ccgggtctct ggtcgcagct cgctggagac gcgcctcgac ctccgggtca
      301 ccggccgcct cccgctgctc caggagcggg agctgtggcg catcgcccaa gaggccgtca
      361 ccaacgtcga gcgccatgcg aaggcgacca aggtggtcgt cacctggcag agcgacgggg
      421 cccgggccct gctcctcgtc gccgacgacg gcatcgggtt ccccacctcg cgggcgggcc
      481 gcctcgacag ctacggcatc gtcgggatgc gggagcgagc ggcgagcatc ggtgccacgc
      541 tcgacatcac ggggaccccc gatgtgggca ccaccgtgcg ctgcgaggtc cgccccatcg
      601 cctgacctgc tgtgccagga tgacgccggc tctggtccgc accacagcaa gagaggaatc
      661 catggccatc cgcctgatgc tcgccgacga ccatcgcatg ctccgtgaag gcctgcgccg
      721 gtccctcacc gacgagggct tcgacgtcat cggcgaggca tccgacggcg aggaggcggt
      781 gcggctggcg ggctcgttgc atcccgacgt cgtcttgatg gacgtcacca tgcccgacgt
      841 cgacggggtc gaggccacgc ggcgcatcca ccagcagcac ccggagatcc gggtcgtcat
      901 gttgacgatg cacgccgacc agagcgtgat cgccgaagcc ctgcgcgccg gcgcatcggg
      961 ctacctcgtg aaggactgct ccaccgacga gatcgccgac gcggtccgca tggcggcgaa
     1021 cgacgaggcc acgctgtcac ccgagttcgc cacggcgatg ctcgacgagg tccggcgcat
     1081 cgaccccaac cccaccgatg acgacgccga ccgggtcgtc accaagcgcg aggtcgaggt
     1141 cctccagctc atcgccgacg gatgctccac cccggaggtc gccgagcgcc tgttcatcag
     1201 ccagaagacg gtgaagaacc acctggcgtc gatctaccac aagctcgatg cccgggatcg
     1261 cacgcaggcc gtcctccagg ccgtgcgcat gggcatcgtc cgcctgagct agctggcgtc
     1321 cgtgaccgcc ggcgcgaccg gctctcagcc ctttgcctgg cccgagcgcg ggtggggcgg
     1381 gacgtgcgtc ccaccccacc tggtcggctt ccctccatca tgggcaaccg cttcgaaccg
     1441 gtctccgctc aggtgcagag cgtgtcggct tcgtaggcct tcttgatgca gtcggcggac
     1501 ttgccgaagc cgccgccgtt cgacgttccg aagaaggaca cggccgagaa ggcgacgatg
     1561 gcgatcaggg ccaggagcag ggcgtactcg atgaggcctg cgccacgttc attgtccttg
     1621 tgacgccgac gtgtcgggtg ttggtggcgc atggggagtc cctccacggg tcgcttccct
     1681 gtgaacatcg gcaccaggta tgaggcccta cataggccgt gtggctcaaa ggaccctgga
     1741 ctcacccgtt cggggtgccg gaggggacgg cgaccatcca gtcgtcgtcg cggcggacga
     1801 tcctccaccc gttggccggg tcctcgagcc agtctccgaa ggggctgtcg gcatcccaga
     1861 ggacagcaga agcatcgaga tcgtcgagca ccggtcccgg cttcgtgttg gcgtcgatga
     1921 gctccatgta ctggtcgacc accgacatgg ggtacatgtc gacccggtcg tcgaagaaga
     1981 ccacccggtc ggggccgtac cgggcctcga ggtagttgcc gacgaagtcg cgggtgacga
     2041 cgcgcgagcc cgggctcagc atcccctcct tgcgcatcca ggtgacggag gcctcgggat
     2101 aggtcgcgag gtcggtgtcg ggttgccggg tcacgccgac gaccgcgatc agcacgaaga
     2161 gggcgagcag cgcaccctgg acgggccgca ggaggcgctt gggcgtgtcg cccacgacgt
     2221 cgccgatgcc cgtggccgcc aacgtcatcg ggatgatcag caccaggctg gcctgggcga
     2281 tgttgcggct ggcctgcagc gacagggcgc cgaagacgag caccggcagg accgctcgcc
     2341 accgacgacc gcgccaggcc accagcccga cggcgaggat cagctggaac aggtacatcc
     2401 aggtggcgga cgcgctgagc gacggcgatt cccactccgc ggtgttggcg aacgcatcgc
     2461 tgtgctggag cagcttggcg gggaacgtga tgaggtccca gccgatgggg ctcaccagcg
     2521 cgccgagcgc ggtgcccagc gccgcccacc ccagcacgcg gagctccacc gtggg
//
LOCUS       DATMFS010000118         3857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28268, whole genome shotgun sequence.
ACCESSION   DATMFS010000118 DATMFS010000000
VERSION     DATMFS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28268"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..716)
                     /locus_tag="VNQ33_02220"
     CDS             complement(192..716)
                     /locus_tag="VNQ33_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96943.1"
                     /translation="MKDNRKIPRALAVFAAVGALVMLFATPSSAANERDNVFFSDSGA
                     VCIEGINATTVLGSGYYQFQSATRSLQLDCSTSRSLAANNISTTTGLRIINGTYDTRW
                     DCAWFPKNWNSSTASRVDAPYIQYSSGPIREWCGVDPSSTFVGVGANSYHQARVWGTW
                     WQNGSFRGAVSNLI"
     gene            complement(1262..2026)
                     /locus_tag="VNQ33_02225"
     CDS             complement(1262..2026)
                     /locus_tag="VNQ33_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ96944.1"
                     /translation="MTIEIDLGGQVALVTGGARGVGRGITDRLLAAGATVAVCGRNAP
                     EPGVLPDGVLFVAADVREPESVQAMVEEVVGELGGLHLAVNNAGGSPGTDAATASPRF
                     SDKILSLNLTAALHVSQAANAVMQTQAEGGSIVNIGSLSGLRPSPGTAVYGAAKAGLL
                     SLTTSLAMEWAPTVRVNMVSAGMVRTEIFEDYYGGPEGAAAVSATVPLGRVAEPADVG
                     NAVVWLASPLASFVSGANLTVHGGGEQLAFFDHQRG"
     gene            complement(2110..2775)
                     /locus_tag="VNQ33_02230"
     CDS             complement(2110..2775)
                     /locus_tag="VNQ33_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96945.1"
                     /translation="MSDATTPAQPSATAGSDETVPADANDVIELHRRTIDKILVSFGA
                     LTTVVFLVAGGLLLWGANFSADYVHRELGSQNVFFPSAEELKAEGRDDLVGHAGQQVT
                     TGAQAEAYASYIGGHLEGIADGKTYSEIDDRGAAAAVQKAIDSGASDEEVAKLQATAD
                     QLKAQRDTLFKGETLRGLLLSSYAWSTVGRIAGYAAIAAFAAAVAMGALTIAGLVHLR
                     RRS"
     gene            2953..3753
                     /locus_tag="VNQ33_02235"
     CDS             2953..3753
                     /locus_tag="VNQ33_02235"
                     /inference="COORDINATES: protein motif:HMM:NF005925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWJ96946.1"
                     /translation="MTAESDDPSERPEMGTTSTVHPSGIAEVVMDNPPVNALNVAGWF
                     ELAKVVRGLGEDPEVRVVVLRAEGKGFNAGVDIKEMQATEGYDALIGANRGCYEAFAA
                     VYECKVPVIAAVNGFCVGGGIGLVGNADVIVAADDAVFGLPEVDRGALGAATHLSRLV
                     PQHKMRAMVYTSANATAQELHAFGSVLEVVPPAELRAAAMKVAEAIAAKSPTVIRAAK
                     ESLNGIDLWDVKRSYRFEQGFTFELNLSGVADEHRDAFVAKRDTDVSK"
BASE COUNT          600 a         1268 c         1378 g          611 t
ORIGIN      
        1 gccgtccgca gcgtcccgtg catcttgctc gcggtcaatg gcctggagtt gctctacctt
       61 ggcgttgttg ccaccgtcgt cttgtgcgct gctcgcagtc acgccggcga cgatgcccgc
      121 gcccgaaaga accgtgatcg caacggcgac acgaagtcga gtcagtttca tgtgttgtct
      181 cactttccgt gtcagatgag gttggaaacg gcgccccgga aggacccgtt ctgccaccag
      241 gtaccccaga cgcgggcttg gtggtagctg ttcgcgccga cgccgacgaa ggtggaactc
      301 ggatcaacac cgcaccattc acggatcggg cctgagctgt actggatata cggtgcatcg
      361 acccgggagg cggtactgct gttccagttc ttggggaacc aggcacagtc ccaccgggtg
      421 tcataggtcc cgttgatgat ccgcaggcct gtggtggtgc tgatgttgtt cgccgcaagt
      481 gaacggctgg tcgagcagtc aagctgcaga ctccgcgtcg ctgactggaa ctggtagtag
      541 ccactgccca gcacggtggt ggcgttgatg ccttcgatgc acaccgctcc ggagtcggag
      601 aagaagacgt tgtcgcgctc attggccgcc gaactcggcg ttgcaaagag catgacaagc
      661 gccccgaccg ccgcaaagac cgcaagggcg cgcggaattt tcctattgtc cttcaatatg
      721 acctccccaa aggtctttcc gctcgaggcg ggacgaccgc cgtagccgcc gttacggacg
      781 gtatcggaga gaacgcgata cgtccagcaa gagttgcaaa tacctgcttg aggagccctg
      841 tctgatgtgc cggtacgtct cgatgtcctg cacgttgaga aggttggtgg aagtcgtatg
      901 acctcgggcc gaacggaacc ttgaaagttc cgttcggtgg caaccaaaca ggcaaagccc
      961 ggtggcagtt ctacaggccg tcatgtcccc gagccccgga gggctcgggg acggaccgag
     1021 agttgccgcg cgccagcgtc gagcttgagg agcagcgcgg gtctgtgcgt cgtcaccgtt
     1081 gctctcaggc tgaacgcgtc gagcagggcg tcgatcagtt cgccggtcgg atcgtcgtgg
     1141 tccgggatcg ggcttccgcc gctgctggct cgctgcgatg gtgcgacgag atcgggcgga
     1201 ccggctcgtc gggtcctctg cgcttcgggt gcttcccgtg tccgcggcgc taggccaccg
     1261 ctcagccgcg ctggtggtcg aagaaggcga gctgctcgcc gccgccgtgg acggtgaggt
     1321 tggcgccgct cacgaagctc gccagggggc tggccagcca cacgacggcg ttgccgacat
     1381 cggcgggctc cgccacccgg ccgagcggga cggtcgcgga cacggcggcg gcgccctccg
     1441 gtccgccgta gtagtcctcg aagatctcgg tgcggaccat gcccgccgac accatgttca
     1501 cccggaccgt cggcgcccac tccatggcca ggctggtggt gaggctgagc aggccggcct
     1561 tggcggcgcc gtagacggcg gtgccggggg aggggcgcag gcccgacagc gagccgatgt
     1621 tgacgatcga cccgccttcg gcctgcgtct gcatcaccgc gttggccgcc tgcgagacgt
     1681 gcagggcggc ggtcaggttg aggctgagga tcttgtcgct gaagcgaggc gacgccgtgg
     1741 ccgcgtcggt gcccggcgag ccgccggcgt tgttgacggc gaggtgcagc ccgcccagct
     1801 cgcccacgac ctcctcgacc atggcctgga ccgactcggg ctcgcggaca tcggcggcca
     1861 cgaacaagac gccgtcgggc agcacccctg gctcgggcgc gttgcgcccg cacacggcga
     1921 cggtcgcgcc cgccgcgagg agccggtcgg tgatcccccg gcccacgccc cgggctccgc
     1981 ccgtcaccag cgccacctgc ccaccaaggt cgatctcgat cgtcacgacc gcaagggtgg
     2041 cacaggcgcg cggctgcttc gagcgactgg ctgcttcgaa gcggtcgcgc cggcgtccgg
     2101 gctccgctgc tacgaacggc ggcgcaggtg gacgagaccg gcgatggtga gcgcgcccat
     2161 cgcgacggcg gcggcgaagg cggcgatggc ggcatacccg gcgatgcggc ccaccgtgga
     2221 ccaggcgtag ctcgacagca gcaggccgcg cagggtctcg cccttgaaga gggtgtcgcg
     2281 ctgagccttc agctggtcgg ccgtcgcctg cagcttggcg acctcctcgt cactggcgcc
     2341 cgagtcgatc gccttctgca cggccgctgc cgcgccacgg tcgtcgatct cggagtaggt
     2401 cttgccgtcg gcgatcccct cgaggtggcc tccgatgtag ctggcgtagg cctcagcctg
     2461 cgcgccggtg gtgacctgct ggccggcgtg gcccaccagg tcgtctcggc cttcggcctt
     2521 cagctcctcg gcggagggga agaacacgtt ctgcgagccg agctcgcggt gcacgtagtc
     2581 agcagagaag ttggcgcccc acaggagcag accgccggcc acgaggaaca ccacggtggt
     2641 gagggcgccg aagctgacga ggatcttgtc gatggtccgg cggtggagct cgatcacgtc
     2701 gttcgcatcg gcaggcacgg tctcatcgga cccggcggtg gcggacggct gcgcgggggt
     2761 ggtcgcgtcg ctcatgggag gactccttcg tcgggcggcg gccggacgct ccgaccgtcc
     2821 tggatcctgc gatccgctgc tgacggtacg gaccgcagcc cggtcagcac agaggcgaag
     2881 gtcccggctc gccgacaccc cgggaagccc catcgtcttc gaggaggagg cgctccggac
     2941 gcggcggcta cggtgaccgc cgaatctgac gacccatcag aaaggcccga gatgggcacc
     3001 acgtcgacgg tccaccccag cggcatcgcc gaggtggtca tggacaaccc gccggtgaac
     3061 gcgctgaacg tggccgggtg gttcgagctg gcgaaggtcg tgcgtggcct cggcgaggac
     3121 cccgaggtgc gggtcgtcgt gctgcgcgcc gagggcaagg gcttcaacgc cggcgtcgac
     3181 atcaaggaga tgcaggcgac ggagggctac gacgccctca tcggcgccaa ccggggctgc
     3241 tacgaggcgt tcgccgccgt ctacgagtgc aaggtgccgg tcatcgccgc cgtcaacggg
     3301 ttctgcgtcg gcggcggcat cgggctggtc ggcaacgccg acgtgatcgt ggcggccgac
     3361 gatgccgtgt tcggcctgcc tgaggtggac cggggagcgc tcggcgccgc cacccacctg
     3421 agccggctgg tcccgcagca caagatgcgg gcgatggtct acacgtcggc caacgccacc
     3481 gcccaggagc tgcacgcctt cgggtcggtg ctcgaggtgg tgccccctgc cgagctgcgg
     3541 gctgcggcga tgaaggtggc cgaggccatc gcggccaagt cgcccaccgt catccgggcg
     3601 gccaaggagt cgctcaacgg catcgacctc tgggatgtga agcgctcgta ccggttcgag
     3661 cagggcttca ccttcgagct gaacctgtcg ggtgtggccg acgagcaccg cgacgccttc
     3721 gtcgccaagc gagacacgga cgtgagcaag tgagcgagac cgagacctat cccgcgccca
     3781 aggacaagcg cctgaccgaa gacgaggtcg tcgcccagct gtcgagcggc atgaagatcg
     3841 gcatcggcgg gtggggg
//
LOCUS       DATMFS010000119         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_5228, whole genome shotgun sequence.
ACCESSION   DATMFS010000119 DATMFS010000000
VERSION     DATMFS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5228"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VNQ33_02240"
     CDS             complement(<1..561)
                     /locus_tag="VNQ33_02240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ96947.1"
                     /translation="MAPRPVDHVPIAVADPVVGPEPPVEPAGSLAAVDAAVAGHPRPG
                     RPGYIPALDGLRAVAVLAVVAYHLDELPGGFFGVDVFFVISGFLITRLLLAERERNGG
                     IALGSFWIRRFKRLVPALFVVLVAVSLGSRLWLPAWRLGQIRWDALAGLGYVANWRFV
                     LSGQSYFSSGLAPSPLRHTWSLAIEEQ"
     gene            705..863
                     /locus_tag="VNQ33_02245"
     CDS             705..863
                     /locus_tag="VNQ33_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96948.1"
                     /translation="MWGPAVIVIVLVAAIPIAVVMSGGVASGLLGYFLKEEAEVTHEG
                     SELIETNV"
     gene            complement(879..1226)
                     /locus_tag="VNQ33_02250"
     CDS             complement(879..1226)
                     /locus_tag="VNQ33_02250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWJ96949.1"
                     /translation="MSNPTGLPYRPSVAAGEWVVVSGQVGRRDGAIVEDFAGEVDQLL
                     ANLAAQLAEHGLGMEHVVKTTCFLLDFAQFGEFNERYAAAFPEPRPARSTVQVAGLPL
                     GAHAEVEAWAYRA"
     gene            complement(1226..>2071)
                     /locus_tag="VNQ33_02255"
     CDS             complement(1226..>2071)
                     /locus_tag="VNQ33_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96950.1"
                     /translation="PVPPLAVPDGSWAATLRTSWVEPGYLETDATWCEPGGEPASSLA
                     NGGAFGAKDPAHLGAVARELADRHGRAVRVLWSREDVVRLGPKRPPVAGGADPDGTGR
                     LTVVRTAGIASAIARVAPGLVVDEVDVSGPPTSAAPRAAGWAEALLLLAGARGSLEPV
                     VGPDGGVAEAAVVDGRIQVRVAAGDPLDEVVLRSYAIGAAHMAFGWITSEGLAVDPDG
                     TVHDLTIRSFGIPRAVDTPPIDVEIDPSPGPAVNGSDAVFAAVAAAVWLHQGRPQDLP
                     TRGAR"
BASE COUNT          298 a          745 c          801 g          227 t
ORIGIN      
        1 ctgctcttcg atcgccagcg accaggtgtg ccgcagcggg ctcggggcca gaccggacga
       61 gaagtacgac tggccgctca gcacgaaccg ccagttggcg acgtagccca ggccggcgag
      121 ggcgtcccac cggatctggc cgaggcgcca cgccggcagc cacagccgcg acccgagcga
      181 cacggcgacc agcacgacga acagggccgg gacgaggcgc ttgaagcggc ggatccagaa
      241 cgacccgagg gcgatgccgc cgttgcgctc ccgttcggcg aggagcaaac gggtgatgag
      301 gaagccgctg atgacgaaga agacgtcgac gccgaagaag ccgcctggca gctcgtcgag
      361 gtggtacgcc accacggcca gcacggcgac ggcgcgcagg ccgtcgaggg cggggatgta
      421 gccgggacgg ccgggccggg ggtggccggc gacggccgca tcgacggccg cgagcgatcc
      481 cgccggttcg accggcggct caggcccgac gacgggatcg gcgacggcga tcggcacgtg
      541 gtcgacgggg cggggagcca ccgtcctggg agccacctgc gggcgcgggg tgggcttcgg
      601 gccgcgcccg ttcgggcgtg gaggcggctt cggacccatg acccgaccac ggtaccgccg
      661 gccttcgttg cgctcccagc cacccccgga ccaagatggg gcccatgtgg ggtcccgccg
      721 tcatcgtcat cgtcctggtc gctgcgatcc cgatcgcggt cgtgatgtcg ggcggcgtgg
      781 cgtcgggcct gctcggctac ttcctgaagg aagaggccga ggtcacccac gagggcagcg
      841 agctcatcga gaccaacgtc tgatcgcccg ctgaccggtc aggcccggta cgcccaggcc
      901 tcgacctcgg cgtgggcgcc gagcggcagg ccggcgacct ggacggtcga gcgcgccggg
      961 cggggctcgg ggaacgcggc ggcgtagcgc tcgttgaact cgccgaactg ggcgaagtcg
     1021 agcaggaagc acgtcgtctt caccacgtgc tccatgccga ggccgtgctc ggccagctgg
     1081 gccgccaggt tggcgaggag ctggtcgacc tcgccggcga agtcctcgac gatggccccg
     1141 tcgcgccggc ccacctggcc cgacacgacg acccactcgc ccgcggcgac agagggccgg
     1201 tacggcagtc cggtggggtt cgacatcaac gcgctcctcg ggtgggcaga tcttggggac
     1261 ggccctggtg cagccagacg gcagccgcga ccgcggcgaa cacggcatcg gacccgttca
     1321 cggccgggcc aggcgacggg tcgatctcga cgtcgatggg cggcgtgtcg accgcccggg
     1381 ggatgccgaa cgagcggatc gtcaggtcgt gcacggtgcc gtcggggtcg acggcgaggc
     1441 cctcgctggt gatccagccg aaggccatgt gggcggcgcc gatcgcgtac gagcgcagga
     1501 ccacctcgtc gagcgggtcc ccggcggcca cgcgcacctg gatccgcccg tcgacgacgg
     1561 ccgcctcggc gacgccgccg tcggggccga cgaccggctc gagcgagccg cgggcccccg
     1621 ccaggagcag gagggcctcg gcccagccag cggctcgggg cgcggccgac gtgggcgggc
     1681 cggacacgtc gacctcgtcg accaccagcc ccggtgcgac acgggcgatg gccgaggcga
     1741 tccccgccgt gcgcaccacg gtgagccgcc cggtgccgtc ggggtcggcg ccaccggcga
     1801 cgggtgggcg cttcgggccg aggcgcacca cgtcctcgcg cgaccagagc acccgcacgg
     1861 cgcggccgtg gcggtcggcc agctcccggg ccaccgcacc gaggtgcgcc gggtccttgg
     1921 ccccgaacgc cccgccgttg gcgagcgacg atgccggctc gccgcccggc tcgcaccagg
     1981 tggcatccgt ctccaggtag ccgggctcga cccagctcgt gcgcagggtc gcggcccagg
     2041 aaccgtccgg gaccgccagc ggtggaacgg g
//
LOCUS       DATMFS010000120         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_74685, whole genome shotgun sequence.
ACCESSION   DATMFS010000120 DATMFS010000000
VERSION     DATMFS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74685"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VNQ33_02260"
     CDS             complement(<1..718)
                     /locus_tag="VNQ33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HWJ96951.1"
                     /translation="MSDPSVPDPGGSQLDPPPADDLGPALDAGDMTELLRAAGMGDEQ
                     IAKAREDHHLELLAIEHLVVPEPGEYDLQGIADASGLTPEQITRLWRSLGFAEPQPGD
                     HLFTDVDVDMLRTIGQLLTMGWIDDELTIQMARVIGSSLSRVASALIDSIDPEEPRQP
                     SNRPRTDDGFAAVAPLLLPMLLQVMDYVWRRHVQAEARARMAREHADGDPDHRVVGFA
                     DLVGFTALSQQIDAHELAEVV"
     gene            complement(715..2667)
                     /locus_tag="VNQ33_02265"
     CDS             complement(715..2667)
                     /locus_tag="VNQ33_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96952.1"
                     /translation="MFRRRSAPPVTGDTATVPPAAVSATQLDAAGWVLPGGIAAGQDG
                     RAPAWTLVGTVGSPTATAVDPAGLVVGEAWSLDWWIGGDDRWYVPAREAAVRQDLVAD
                     APIVETRARIPGGDAVHRAYGIRSARQTGDEWVVAEVENQTPVPLAAAFVIRPFMADS
                     LGSVREITVEPVAGGAGRDVAHLVRVDGTPAVVLPRRPARIQAGNLAEGDVVDLVTTN
                     EAGSELVTAACEAGMATMAFLFPVPHTALVRAMVPVGAVDPAEAVAYPVVAPDAEAVA
                     GGWEIHRRGPRFEIPERRIAMAVERARTQVLLAHDGQAVRRDGAEEPDVEPGATDPIL
                     HAFDLLDKPDDVMNVVARWQERLADAPPGFDAIALGLLSTHWLLHRDDPLLDWMLPEV
                     AAAVERLDRADRKGRLAYPADRARASAALRTASLVLAQRGQTSAALQVERLAARLAPG
                     TAPEPASAADRLVAAATALAVGDLRAHDDLLRTIGEASSTGAWSGPGRHGRGIGHDLA
                     ASGAMIAAGRAMLVAERPDGLALLPAFPDSWYGNPVELHDQPTSFGVISFAIRWHGRR
                     PALLWELVSHPGLGPVVLTVPGLDPTWSTAELRGDALLAEVDPPEGIDLVREVAEHPG
                     LQEHMRPEGDEPEAPGPRLLDGGTFS"
     gene            complement(2680..>2991)
                     /locus_tag="VNQ33_02270"
     CDS             complement(2680..>2991)
                     /locus_tag="VNQ33_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWJ96953.1"
                     /translation="SGLAGMGDLVATCISPQSRNRHVGELLGTGQTIDEIIASMNMVA
                     EGVKTSKVVMELSNELGIDMPIGSEVYACCHEGRTAAEAYTGLLRRGTGREMDGISPA
                     G"
BASE COUNT          387 a         1071 c         1153 g          380 t
ORIGIN      
        1 ccaccacctc ggccagctcg tgggcgtcga tctgctggct cagcgcggtg aagcccacca
       61 ggtcggcgaa cccgacgacg cggtggtccg ggtccccgtc ggcgtgctcg cgcgccatgc
      121 gggcgcgggc ctcggcctgg acgtggcggc gccacacgta gtccatgacc tgcaggagca
      181 tcgggagcag cagcggggcg acggcggcga acccgtcgtc ggtgcgcggg cggttggacg
      241 gctggcgggg ctcctcggga tcgatgctgt cgatgagcgc gctggcgacg cgcgacagcg
      301 acgagccgat gacccgcgcc atctggatcg tgagctcgtc gtcgatccac cccatcgtca
      361 ggagctggcc gatggtgcgg agcatgtcga cgtcgacgtc ggtgaagagg tggtcgccgg
      421 gctggggttc ggcgaagccg agcgagcgcc acagccgggt gatctgctcg ggggtgaggc
      481 cgctggcgtc ggcgatgccc tggaggtcgt actcccctgg ttcgggcacc acgaggtgct
      541 cgatggcgag cagctccagg tggtggtcct cccgcgcctt ggcgatctgc tcgtcgccca
      601 tgccggcagc gcgcagcagc tcggtcatgt cgccggcgtc gagggccggg ccgaggtcgt
      661 cggcgggcgg cgggtcgagc tgcgacccgc ccggatcggg cacggacgga tcgctcacga
      721 gaaggtcccg ccgtcgagga gccggggtcc gggcgcctcg ggctcgtcgc cctcgggacg
      781 catgtgctcc tgcaggcccg ggtgctcggc gacctcgcgc accaggtcga tcccctcggg
      841 cgggtcgacc tcggcgagca gggcgtcgcc ccggagctcg gcggtggacc acgtggggtc
      901 gagaccgggg acggtcagca ccaccggccc gagtcccgga tgcgagacga gctcccacag
      961 cagcgccggg cgacggccgt gccagcggat ggcgaaggag atcacgccga acgacgtggg
     1021 ctggtcgtgc agctcgacgg ggttgccgta ccaggagtcg gggaaggcgg gcagcagggc
     1081 cagcccatcg ggccgctcgg cgacgagcat cgcccggccg gcggcgatca tggcgccgga
     1141 tgcggccagg tcgtggccga tgccgcgccc gtggcgaccc ggtccggacc aggcaccggt
     1201 gctgctcgcc tccccgatcg tccggagcag gtcgtcgtgc gcccgcaggt cacccacagc
     1261 gagcgcggtg gccgccgcca ccaggcggtc ggccgccgag gcgggctcgg gcgccgtccc
     1321 gggggcgaga cgggccgcca gccgctcgac ctgtaacgca gcagaggtct ggccgcgctg
     1381 cgccagcacg agcgaggccg tccgcagcgc tgccgaagct cgggcccggt cggccgggta
     1441 cgcgaggcgg cccttgcgat cggcacggtc gaggcgctcg accgcggcgg ccacctcggg
     1501 gagcatccag tcgaggagcg ggtcgtcgcg gtggagcagc cagtgcgtgc tgagcagccc
     1561 gagggcgatg gcatcgaaac cgggcggagc gtcggccagc cgctcctgcc agcgcgccac
     1621 gacgttcatc acgtcgtcgg gcttgtcgag gaggtcgaag gcgtggagga tcgggtcggt
     1681 cgcacccggc tcgacgtcgg gctcctcggc tccgtcgcgc cgcaccgcct ggccgtcgtg
     1741 ggcgagcagc acctgggtcc gagcccgctc gaccgccatg gcgatgcgtc gctccgggat
     1801 ctcgaagcgg gggccgcgcc ggtggatctc ccaaccgccg gccaccgcct cggcatccgg
     1861 agcgacgacc gggtaggcga cggcctcggc ggggtcgacg gcgccgaccg ggaccatcgc
     1921 ccggacgagg gcggtgtgcg gcaccgggaa caagaaggcc atggtggcca tgcccgcttc
     1981 gcaggcagcg gtcaccagct ccgaaccggc ctcgttcgtg gtgacgaggt cgaccacgtc
     2041 gccttcggcc aggttcccgg cctggatccg agccgggcgc cggggcagga ccacagcggg
     2101 tgtgccgtcg acccgcacca ggtgggccac gtcgcggccg gcgccgcccg cgaccggctc
     2161 gacggtgatc tcgcggaccg agccgagcga gtccgccatg aaggggcgga tgacgaacgc
     2221 tgcggccagc ggcacgggtg tctggttctc gacctcggcc accacccact cgtcgccggt
     2281 ctggcgagcc gagcggatcc cgtaggcgcg gtgcacggcg tcgccgccgg ggatccgggc
     2341 ccgggtctcg acgatcggcg cgtcggccac gaggtcctgg cgcacggcgg cctcgcgggc
     2401 cggcacgtac cagcggtcgt cgccgccgat ccaccagtcc aggctccacg cctcgcccac
     2461 cacgaggccg gcgggatcga ccgcggtcgc cgtgggcgag ccgacggtcc ccaccagcgt
     2521 ccacgccggc gcacgaccgt cttgacctgc ggcgatgccg ccagggagga cccatccggc
     2581 cgcgtcgagc tgggtggcgc tgacggcggc gggcgggacc gtggcggtgt cgccggtcac
     2641 gggcggggca gagcggcggc ggaacatgac ccccggggtc tatccggccg ggctgatccc
     2701 gtccatctcg cgcccggtgc cccggcgcaa caggccggtg tacgcctcgg ccgcggtccg
     2761 gccctcgtgg cagcacgcgt agacctccga gccgatgggc atgtcgatcc ccagctcgtt
     2821 cgacagctcc atcaccacct tcgacgtctt cacgccctca gccaccatgt tcatcgaggc
     2881 gatgatctcg tcgatcgtct ggcccgtgcc gagcagctcg cccacgtgcc ggttgcggct
     2941 ctgcgggctg atgcacgtgg ccacgaggtc acccatgccg gccaggccgg a
//
LOCUS       DATMFS010000121         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28375, whole genome shotgun sequence.
ACCESSION   DATMFS010000121 DATMFS010000000
VERSION     DATMFS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28375"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VNQ33_02275"
     CDS             complement(<1..1224)
                     /locus_tag="VNQ33_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWJ96954.1"
                     /translation="MALHKVMGIETEYGIVVRGTAESNPVSASSLLINAYVQELERQR
                     LAGPKVGWDFEDEHPGNDARGSSGPSSPPDVETHLVNAVLTNGARYYVDHAHPEISTP
                     ECADARAVVVFDRAAEEIVKQSIVAANRFLPEGQELVVHKNNSDGKGNSYGCHENYLM
                     ARSTPFSRIVTHATAHFVSRQIFTGSGKVGSEAPGVSAADVPYQLTQRADFFEAEVGL
                     ETTLKRPIVNTRDEPHADAQKYRRLHVITGDANRSETATFLKVGTTAFVLALVEDEAL
                     PRTFAFRSAVAAMRQVSYDLTLRSPLELADGTTITALEIQWELLDRSKKWAEAHDLEP
                     VGGEVGSLVLAEWEAVLTALERDPMELADRLDWVAKMRLLDGYSDRHGIGWDDQRLAA
                     LALQYHDMRPERSLAD"
     gene            complement(1266..>2474)
                     /gene="arc"
                     /locus_tag="VNQ33_02280"
     CDS             complement(1266..>2474)
                     /gene="arc"
                     /locus_tag="VNQ33_02280"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HWJ96955.1"
                     /translation="LAEFRTGLQTRSVLKGQVVSLTVDPFGDSGSGVTFHERPRLSAA
                     DVVLPEGLLERISRHAVELGGHREQLQALGQHLKRGILLYGPPGCGKTLIAKAVANSL
                     AKKVAEKTGDDKGQSYFINIKGPELLNKYVGETERQIRLVFQRAREKSEEGWPVIVFF
                     DEMDSMFRTRGSGISSDMESTIVPQLLSEIDGVEGLRNVIVIGATNREDLIDPAILRP
                     GRLDVKIKIERPNEQAAAQIFGRYLTSDLPLHPDEVRDLGGGDPDKCVQAMIERTVEE
                     MYRDDEDNQFLEVTYQNGDKEIMYFKDFSSGAMIENIVRRAKKLAIKRLISEGAKGIK
                     TQDLLDSIHQEYKEHEDLPNTTNPDDWAKISGKKGERIVYIRTLITHGDEEEPAGGRA
                     IERVGTGQYL"
BASE COUNT          331 a          834 c          856 g          453 t
ORIGIN      
        1 gtcggccagg gaccgctcag gccgcatgtc gtggtactgg agggccaggg ccgccaggcg
       61 ctggtcgtcc cagccgatgc cgtgccggtc gctgtagccg tcgagcaggc gcatcttggc
      121 gacccagtcg agccggtcgg ccagctccat cgggtcccgc tccaaggcgg tgaggacagc
      181 ctcccactcg gcgagcacga gggatccgac ctcgccgccg acgggctcca ggtcgtgcgc
      241 ctcggcccac ttcttcgacc ggtccaggag ctcccactgg atctcgaggg cggtgatggt
      301 ggtgccgtcg gccagttcga gcggcgatcg cagggtgagg tcgtaggaca cctgccgcat
      361 ggcggccacg gcgctgcgga aggcgaaggt ccgcggcagc gcctcgtcct ccacgagcgc
      421 caggacgaac gccgtggtgc cgaccttgag gaacgtcgcc gtctccgaac ggttggcgtc
      481 gccggtgatg acgtggagcc gccggtactt ctgggcgtcg gcgtggggct cgtcgcgcgt
      541 gttcacgatg gggcgcttga gcgtggtctc cagcccgacc tcggcctcga agaagtccgc
      601 ccgctgggtg agctggtagg gcacgtccgc cgccgagacg ccgggcgcct cggacccgac
      661 cttgccgctg ccggtgaaga tctggcggct gacgaagtgg gcggtggcgt gggtgacgat
      721 ccgcgagaac ggcgtcgacc gggccatcag gtagttctcg tggcagccgt aggagttgcc
      781 cttgccgtcg gagttgttct tgtggaccac cagctcctgg ccctcgggca agaagcggtt
      841 cgcggcgacg atggactgct tgacgatctc ctccgccgcc cggtcgaaga cgaccacggc
      901 gcgggcgtcg gcgcactccg gggtggagat ctccgggtgg gcgtggtcga cgtagtaccg
      961 ggcgccgttc gtgagcacgg cgttgacgag gtgcgtctcg acgtccggtg gcgacgacgg
     1021 gcccgacgac ccccgggcgt cgttgcccgg gtgctcgtcc tcgaagtccc accccacctt
     1081 cgggccggcc agccgctgcc gttcgagctc ttggacgtag gcgttgatga gcagggagga
     1141 ggcgctcacc gggttggact ccgccgtgcc gcggacgacg atcccgtact cggtctcgat
     1201 gcccatcacc ttgtgcagcg ccatgcccgg gaccctaccg ccgcgctccg gcggcctggc
     1261 cccgcctaga ggtactggcc ggtgccgacg cgttcgatgg cgcggccacc ggcgggctct
     1321 tcttcgtctc cgtgggtgat gagggtgcgg atgtagacga tccgctcgcc cttcttgccc
     1381 gagatcttcg cccagtcgtc cgggttcgtc gtgttgggga ggtcttcgtg ctccttgtac
     1441 tcctggtgga tcgagtcgag caggtcctgg gtcttgatgc ccttggcccc ctcgctgatc
     1501 aggcgcttga tggcgagctt cttggcccgg cggacgatgt tctcgatcat ggcccccgac
     1561 gagaagtcct tgaagtacat gatctccttg tcaccgttct ggtacgtgac ctcgaggaac
     1621 tggttgtcct cgtcgtcgcg gtacatctcc tcgacggtgc gctcgatcat cgcctggacg
     1681 cacttgtcgg gatcgccgcc gccgagatcg cggacctcgt cggggtggag cgggaggtcc
     1741 gacgtcaggt agcgcccgaa gatctgcgcc gcggcctgct cgttcgggcg ctcgatcttg
     1801 atcttcacgt cgaggcggcc gggccgcagg atcgccggat cgatcaggtc ctcgcggttc
     1861 gtcgcgccga tcacgatcac gttgcggagg ccctcgacgc cgtcgatctc cgacagcagc
     1921 tgcggcacga tcgtcgactc catgtccgag ctgatccccg agccgcgggt gcggaacatc
     1981 gagtccatct cgtcgaagaa gacgatgacc ggccagccct cctcgctctt ctcccgcgcc
     2041 cgctggaaca ccagacggat ctgccgctcg gtctcgccga cgtacttgtt gagcagctca
     2101 ggacccttga tgttgatgaa gtagctctgg cccttgtcgt cgccggtctt ctcggcgacc
     2161 ttcttggcca gcgagttggc gaccgctttg gcgatcaacg tcttgccgca gcccggcggc
     2221 ccgtacagca ggatgccccg cttcaggtgc tggcccagtg cctgcagctg ctcccggtgt
     2281 ccgccgagct ccaccgcatg ccggctgatc cgttccagca gcccctcggg caggactacg
     2341 tcggcggcgg acagccgcgg ccgttcgtgg aaggtcactc cggaccctga atccccgaag
     2401 ggatccacgg tcagcgagac gacctgcccc ttgagcacgc tgcgggtctg caggccggta
     2461 cggaattccg ccag
//
LOCUS       DATMFS010000122         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_97979, whole genome shotgun sequence.
ACCESSION   DATMFS010000122 DATMFS010000000
VERSION     DATMFS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97979"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1982
                     /locus_tag="VNQ33_02285"
     CDS             447..1982
                     /locus_tag="VNQ33_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ96956.1"
                     /translation="MTDTPWQQDASSLVDAFRAGDRTPLEELDATLATIEASNLNCWS
                     HLDPERARKAAKEADLTKPFGGVPTGIKELEGVVGWPDTGASLVFKDRIATETSHHVQ
                     RLFDNGGVVPVGLTTSSEFGGLNVSVTKLNGVAHNPWNYDKTVGGSSGGSAAAVSGGH
                     VSLACGGDGGGSLRIPAGYTGLLGFKGTFGRISRGPDAYARPNTVVLGNVSRSVRDSA
                     RYFDVCGGVDTWDPTSLPAKSFEKGLGTQDLKGRKVTIVPGLGGVKLEPGIEEALRAE
                     AEAMIADTGMVLVDHPVNPPNLAAQWMMGNLSTLLAELGPLWPRCADDLTDEVATGVF
                     LAQAMYNLHTAAVAEKLRVAAQREMAAAFDEVDFIIAATNPGPAFPATATTSNSESDL
                     LDMVQSSQAAKYVGRGALFATRVASSFAPKLPNLILDYASSKFPDMVTMGALTIISNV
                     YGNPAVSIPAGTVDDLPVGMQVLGKHHQDELLFDVALSVERNRPWPMVAPPVAAPTTA
                     TAV"
     gene            complement(2495..4066)
                     /locus_tag="VNQ33_02290"
     CDS             complement(2495..4066)
                     /locus_tag="VNQ33_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ96957.1"
                     /translation="MTALLADIVAARGDEPAVIDERGATSWNVLDDRVTRLVHALEDR
                     GLRVGDTVVAMLGNQVELIEVTLACAHGGWLMVPLNWHWVAREVAYVLTDSDAAAVVV
                     EERWADVMTHAMIDAEVSRVRCRLFAGDGPMPSGFERYDDALASASNAELIDPQRGGP
                     MFYTSGTTGRPKGVRSALSAIGGPPEVLMLIAHTVAPTIGVEPGDRIVQLVCGPLYHS
                     AQWAFGLFALCCGATLVLQHRFDAEEVLALIDEHGVTNVHLVPTQMIRLLELPDDVRA
                     GFDGSSLVQIIHGAAPCSPSVKRRMIEWVGPKVTEYYGGTEGGFISLISAEEWLERPG
                     SVGRALTVVEIVILGADGEVLGPGQTGEVWFRNLLGSDFEYHNAPEKTRDAHRDGGLG
                     TLGDIGYLDEAGYLFLSDRKIDMIVSGGVNIYPAEIEAVLGDHRAVADAAVFGVPDEE
                     MGESVHVALVLRHGHVWSDALADELAAFCRERLAGYKVPRSFEVHDGLPRSEAGKLAK
                     RTLRDPHWVHRDRAI"
BASE COUNT          630 a         1551 c         1368 g          570 t
ORIGIN      
        1 accaccccgg ccactgggcc gtccgccaga tcctccacga tcccgccgac gaccacgact
       61 ggcgcatccg tgccctcgtc gacctcgccg ccagcgacga agaaggccga gccgtcatcc
      121 acgtcctcga cgtctcaccc accccctgac ccgggtcacc cccgccaggc ccgccaccat
      181 cctccaaggc ccgccaccca ccaggccccg ccaccccgcc aaggcccgcc accatcctcc
      241 aacttggagc ctggtggtcg cataccgcct cgatcctcca agttggagga tcggagcgca
      301 agatggagga tcgtggtcgc atggggaccc tttccctcca agtttcggcg gcggtcatcg
      361 agggcgggag gaggacgtga ccgaattcat ccgaccgtcg gtcggttttt cttgagcgat
      421 cgctcggacc gcgagagact gtctcgatga ccgacacccc atggcagcag gacgccagct
      481 ccctcgtcga cgccttccgc gccggcgacc gcaccccgct cgaggagctc gatgcgacgc
      541 tggccacgat cgaggcctcc aacctcaact gctggtcgca cctcgatccc gagcgggcgc
      601 gcaaggcggc caaggaggcc gacctgacga agccgttcgg cggcgtgccc acgggcatca
      661 aggagctcga gggcgtcgtc ggctggcccg acaccggcgc ttcactggtc ttcaaggacc
      721 gcattgccac cgagaccagc caccacgtcc agcgcctgtt cgacaacggc ggcgtggtgc
      781 cggtgggcct caccacctcc agcgagttcg gcggcctcaa cgtctcggtc accaagctca
      841 acggcgtcgc ccacaacccg tggaactacg acaagacggt cggcgggtcg tcgggcgggt
      901 cggcggccgc cgtctccggc ggccacgtga gcctcgcctg cggcggcgac ggcggcggct
      961 cgctgcgcat ccccgccggc tacaccggcc tgctcggctt caagggcacg ttcggccgca
     1021 tcagccgcgg gcctgacgcc tacgcccgcc ccaacacggt cgtgctcggc aacgtgtccc
     1081 gctcggtgcg ggactccgcc cgctacttcg acgtgtgcgg tggggtcgac acgtgggacc
     1141 cgacctcgct gcccgccaag agcttcgaga agggcctcgg cacgcaggac ctcaagggcc
     1201 gcaaggtcac catcgtccca gggctcggcg gcgtgaagct cgaacccggc atcgaagagg
     1261 cgctgcgcgc cgaggccgag gcgatgatcg ccgacaccgg catggtcctc gtcgaccacc
     1321 cggtcaaccc gccgaacctg gccgcccagt ggatgatggg caacctctcc acgctgctcg
     1381 ccgagctcgg tccgctctgg ccccgctgcg ccgacgacct caccgacgag gtcgccaccg
     1441 gcgtgttcct cgcccaggcg atgtacaacc tgcacaccgc ggcggtggcc gagaagctcc
     1501 gggtcgccgc ccagcgggag atggccgccg cgttcgacga ggtcgacttc atcatcgccg
     1561 ccaccaaccc cggcccggcc ttcccggcca cggccaccac cagcaactcc gagtcggacc
     1621 tgctggacat ggtgcagtcg agccaggcgg ccaagtacgt gggccgtggc gccctgttcg
     1681 ccacccgcgt ggcgtcgtcg ttcgcgccga agctgccgaa cctgatcctc gactacgcgt
     1741 cgtccaagtt ccccgacatg gtcaccatgg gcgccctcac gatcatctcg aacgtgtacg
     1801 gcaacccggc ggtgtcgatc ccggctggca cggtcgacga cctccccgtc ggcatgcagg
     1861 tcctcggcaa gcaccaccaa gacgagctgc tcttcgacgt cgccctctcg gtggagcgca
     1921 accgcccctg gccgatggtc gccccgcccg tcgccgcccc caccaccgcc accgccgtct
     1981 gacccacacc gctttcgccc aggcgcgatg cccgcgctcg ggcgcgatgc ccgagatcca
     2041 ggtcggtgcc gtgccctggt ggcgggttcc atcgccgtca tcgcctgtcg ctgctcgcgg
     2101 cctcgggtgg ccgagcacac gcgcccgggg cctcatcgcg ctcgtccggg ttgctcgccg
     2161 ctctcgtcac ctcgacctca ccaactgcgc gcagatcggg gcatctcgca gatttcgggt
     2221 cacgcgtgcc ccgccacggg gcacagatga cccgaaatcg cgtggatcgc cagaagatgg
     2281 ctggttccga ccacgctggg taggcaggag cggcggagac gtgggaacag cgccaacgcg
     2341 ggccccgctc gcctgggcgc gtgggttcca accggggtgc gtcgaacgag gtcccgatcc
     2401 catcggggat gggagcgtcg gacggtcaga accgatcacc atgccgccat gtcgccagac
     2461 cgggttcagt cgccagcccg accccgtcga aaggtcagat ggcgcggtcg cggtgaaccc
     2521 agtgggggtc gcggagggtg cgcttggcga gcttgccggc ctcgctgcgg gggaggccgt
     2581 cgtggacctc gaagctgcgc gggaccttgt agccggcgag ccgctcccgg cagaacgcgg
     2641 cgagctcgtc ggcgagggcg tcggaccaga cgtggccgtg gcgcaagacg agggcgacgt
     2701 ggacggactc gcccatctcc tcgtcgggga cgccgaagac ggcggcatcg gcgaccgccc
     2761 ggtggtcgcc gagcacagcc tcgatctccg ccgggtagat gttcacgccc cccgacacga
     2821 tcatgtcgat cttgcggtcg ctcaagaaca ggtagccggc ttcgtcgagg tacccgatgt
     2881 cgcccagcgt cccgaggccg ccgtcgcggt gcgcgtccct cgtcttctcc ggggcgttgt
     2941 ggtactcgaa gtccgagccg agcaggttgc ggaaccacac ctcgccggtc tgccccggcc
     3001 cgagcacctc gccgtcggca ccgaggatga cgatctcgac gaccgtgagc gcacggccca
     3061 cgctgccggg gcgctccagc cactcctcgg cggagatgag cgagatgaac ccgccctcgg
     3121 taccgccgta gtactcggtg accttcgggc cgacccactc gatcatccgc cgcttcaccg
     3181 acggcgagca cggcgccgcg ccgtggatga tctgcacgag cgacgagccg tcgaagccgg
     3241 cccggacgtc gtcgggcagc tcgagcaggc ggatcatctg ggtggggacg aggtggacgt
     3301 tggtgacgcc gtgctcgtcg atgagggcca gcacctcctc cgcgtcgaag cggtgctgga
     3361 gcacgagcgt ggccccgcag cagagggcga agaggccgaa ggcccactgc gccgagtggt
     3421 acagcggacc gcacaccagc tgcacgatcc ggtcaccggg ctccacgccg atggtgggtg
     3481 ccacggtgtg ggcgatgagc atcagcacct cgggcggccc gccgatggcg ctgagcgccg
     3541 accgcacgcc cttgggccgg cccgtcgttc ccgatgtgta gaacatcggc ccgccgcgct
     3601 gggggtcgat cagctcggcg ttgctcgccg aggcgagggc gtcgtcgtag cgctcgaagc
     3661 ccgagggcat cggcccgtcg cccgcgaaga gccggcaccg cacgcgcgac acctcggcgt
     3721 cgatcatggc gtgggtcatg acgtcggccc agcgctcctc gaccacgacg gcggcggcat
     3781 ccgagtcggt cagcacgtac gccacctccc gggccaccca gtgccagttg agcggcacca
     3841 tcagccagcc gccgtgggca caggcgagcg tgacctcgat gagctcgacc tggttgccca
     3901 gcatcgccac cacggtgtcg ccgacgcgca ggccgcggtc ctcgagcgcg tgcaccaggc
     3961 gcgtgacccg gtcgtcgagc acgttccacg acgtcgcgcc gcgctcgtcg atgaccgccg
     4021 gctcgtcgcc acgcgccgcg acgatgtccg ccaagagggc ggtcacggga ggaagctccc
     4081 gtgacactcg aacggcaccc gccgcggcag gtgcacccg
//
LOCUS       DATMFS010000123         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_307076, whole genome shotgun sequence.
ACCESSION   DATMFS010000123 DATMFS010000000
VERSION     DATMFS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307076"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VNQ33_02295"
     CDS             <1..443
                     /locus_tag="VNQ33_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96958.1"
                     /translation="AEAVDPDQPLTVGVYEAVTRHPERASAVAQLALERSDVVSFHCY
                     ADEAGLTRAINGLAAHGRPLLCTEWLGRPTSPVALAEAFRDRGVSAFCWGLVDGRTQT
                     RHPWTSWLRPPTSGAPWFHELLHADGTPYDPDEAALLRRVSPRR"
     gene            529..>2007
                     /locus_tag="VNQ33_02300"
     CDS             529..>2007
                     /locus_tag="VNQ33_02300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HWJ96959.1"
                     /translation="MTTTDADLAGIDASIRPQDDLFGHVNGAWLATAEIPADLAVSGG
                     FIDLALEAEEIVGDLLTEAREAVAAGGEVTADQRRLGDLSTSFGDEERIEALGAAPIA
                     DDLAAIAALRSAADVVRLLGRLQREGSGGLVDAYVNTDDRQSDRNIVNLVQGGIGLPD
                     ESFYREDGFAEIRTAYETHVARVLELIGLDADQAADGAARVLALETRLAAGHWDRVRS
                     RDVIASYNLTSFAGLTELAPAYDWAAWAEGLGATGDELAEVLVRQPSYLEALSTALDE
                     VALSDWQAWLSYHVAIGAAPYLSTPFVDEHFSFYSRTLKGVTEQRDRWKRGVALANSI
                     LGEAVGALYVAKTFPPDAKAQMDELVANLVEAYRVSIRRLPWMSEETRERALAKLDRF
                     RPKIGYPDTWRDYSALEIRADDLLGNIRRGSAFETARQLAKIGAPVDRDEWLMTPQTV
                     NAYYNPGVNEICFPAAILQPPFFDPEADPAYNYGGIGAVIGHE"
BASE COUNT          285 a          810 c          655 g          257 t
ORIGIN      
        1 gggccgaggc cgtcgacccc gaccagcccc tgaccgtggg cgtgtacgag gcggtgaccc
       61 gccaccccga acgcgccagc gccgtcgccc agctcgcgct ggagcgctcc gacgtcgtct
      121 cgttccactg ctacgccgac gaggcgggtc tcacccgggc catcaacggg ctcgcggccc
      181 acgggcgccc cctgctctgc accgagtggc tgggccggcc gacctcgccg gtcgcgctgg
      241 ccgaggcgtt ccgcgaccgg ggtgtcagcg ccttctgctg gggcctcgtc gacggccgca
      301 cgcagacgag gcacccgtgg acctcgtggc tgcggccgcc cacgtccggg gcgccgtggt
      361 tccacgagct gctccacgcc gacgggaccc cctatgaccc cgacgaggcc gccctgctgc
      421 gccgggtgtc gcctcggcgc tgacccaccg tcgagacgca ccggaggccg gtacgatgcg
      481 cggcctcgcc gcacctgcgg accccgacga cgtgacggag acagcaccgt gaccaccacc
      541 gacgccgacc tcgccggcat cgacgccagc atccgccccc aagacgacct gttcgggcac
      601 gtcaacgggg cgtggctcgc cactgccgag atccccgccg acctggccgt gagcggcggc
      661 ttcatcgacc tggccctcga agccgaggag atcgtcggcg acctgctgac cgaggcccgg
      721 gaggccgtcg ccgccggtgg cgaggtcacc gcagaccagc gcaggctcgg cgacttgtcc
      781 accagcttcg gcgacgagga gcgcatcgag gcgctcggag ccgcgcccat cgccgacgac
      841 ctggccgcca tcgccgccct ccgctccgcc gccgacgtcg tgcggctgct cggccgactc
      901 cagcgcgagg gctccggcgg cctggtcgac gcctacgtca acaccgacga ccgccagtcc
      961 gaccgcaaca tcgtgaacct cgtgcagggt ggcatcggcc tgcccgacga gtcgttctac
     1021 cgggaggacg gcttcgccga gatccgcacc gcctacgaga cccacgtggc ccgcgtcctc
     1081 gagctcatcg ggctcgacgc ggaccaggcc gccgacggcg cggcccgcgt cctggcgctc
     1141 gagacccgcc tcgccgccgg gcactgggac cgggtccgct cccgcgacgt gatcgcgtcg
     1201 tacaacctga cctccttcgc cgggctcacc gagctggcac ccgcctacga ctgggccgcc
     1261 tgggccgagg gcctgggtgc caccggcgac gagctggccg aggtgctggt ccgccagccc
     1321 agctacctcg aggcgctctc gaccgcgctg gacgaggtcg cgctttccga ctggcaggcc
     1381 tggctctcgt accacgtcgc catcggggcg gcgccgtacc tgtcgacccc gttcgtggac
     1441 gagcacttct ccttctactc ccgcaccctc aagggcgtca ccgagcagcg cgaccgctgg
     1501 aagcgcggcg tcgccctcgc caacagcatc ctcggcgaag ccgtcggcgc cctctacgtg
     1561 gccaagacct tcccgcccga cgccaaggcc cagatggacg agctggtcgc caacctcgtc
     1621 gaggcctacc gcgtcagcat ccgccggctg ccgtggatga gcgaggagac ccgcgagcgt
     1681 gccctcgcca agctcgaccg gttccgaccg aagatcggct accccgacac ctggcgcgac
     1741 tactcggcgc tcgagatccg cgccgacgac ctgctcggca acatccgccg gggctcggcg
     1801 ttcgagacgg cccgccaact ggccaagatc ggcgcccccg tcgaccgcga cgagtggctg
     1861 atgaccccgc agaccgtgaa cgcctactac aacccgggcg tcaacgagat ctgcttcccg
     1921 gccgccatcc tccagccgcc gttcttcgat cccgaggccg accccgccta caactacggc
     1981 ggcatcggcg cggtgatcgg ccacgag
//
LOCUS       DATMFS010000124         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_51427, whole genome shotgun sequence.
ACCESSION   DATMFS010000124 DATMFS010000000
VERSION     DATMFS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51427"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..571
                     /locus_tag="VNQ33_02305"
     CDS             281..571
                     /locus_tag="VNQ33_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96960.1"
                     /translation="MATTIRSVAVVASVLLAGACGGEQAPSSQAAIEGWAHDDLGISN
                     AETCLAPYIDKVDIGGVTRAELRAKGIVAVPPEVSQRVANALMVCGLLASPR"
     gene            568..1941
                     /locus_tag="VNQ33_02310"
     CDS             568..1941
                     /locus_tag="VNQ33_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ96961.1"
                     /translation="MSRGLRAALLPLIIGAALVALGHRLGWLVLIMALARYQLAYAAP
                     AADAAVDRGIRQVVRAVGHAFTAVLFVVPALLAVLVGVVGRLVGSDPLHRTTGWSPVP
                     SADGSGAERSRGRTPRLALAATAIILLAATTVWVVRDRSSAGTGKTVSAIVPRAEALQ
                     GQEDIDEVLEEEGAVFRSYQPDPVVGWNLPERFDGSAVSVEDHRRTTVQAADRGPLVW
                     LFGGSTMFGSNQRDDRTIASRFAAISTANGRPVRVVNWGRHAFTAYQQAQLLQHELRR
                     ADELPDLVVFYDGFNDVAVGVGTSLLEGPSTEPPRAPSIAEGSPAEPSRGRPRWMTPA
                     QRYDQIAADYRKAGDLARRAAAAHGIDVVRVWQPSAFTRRPLTRGERVQLARLGIDDQ
                     ALQTLRTAHEEVGRRLPPSVIDLSGALDDAPTVFVDEVHTNERGAELVAEALESALAG
                     RVRDLEP"
     gene            complement(1971..>2270)
                     /locus_tag="VNQ33_02315"
     CDS             complement(1971..>2270)
                     /locus_tag="VNQ33_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96962.1"
                     /translation="VQPLQLDVGPEGSIRSIDPGHLADLGVEHLVGGGIDELFVWLHA
                     AFGRPADSSYEEHRVDRGDRLLRFESSDQETEVRILSERVGDHADWLLATRSAPH"
BASE COUNT          312 a          787 c          834 g          337 t
ORIGIN      
        1 ccatcccttc aatgcgtggt ctcctcgctc gccccaggcg cgatcacact actggggggc
       61 cttgatctgg ctccggtcac cgggcagatc cccgctctgg ggtctggtgg accggggccc
      121 gctggcgtca ccgctgtgag cgggatcggc gaacctggga cggtcgctcg cgtcggtagc
      181 cgcggtgacg gcgtcgagcg ggggcaggcc cggtactgtc gtgccggtgt tgcaccgtcc
      241 tgggtccaac ggtgtcccgc ggccccgatc gtgccggcgg atggccacga cgatccgctc
      301 ggtcgccgtc gtggcgtcgg tcctgctcgc cggggcatgc ggtggggagc aagcgccgtc
      361 gtcccaagca gcgatcgaag gctgggctca cgatgacctc ggcatctcga acgcagagac
      421 ctgcctcgcc ccctacatcg acaaggtcga catcggggga gtgacgcgag cagagctccg
      481 ggccaagggc atcgtcgccg tccctccgga ggtcagccag cgcgtcgcca acgcgctcat
      541 ggtgtgcggt ctgctggcct cccccagatg agccgagggc tgcgcgccgc gctcctgccg
      601 ctgatcatcg gcgctgcact ggtcgcgctg gggcaccggc tcggatggct cgtgctgatc
      661 atggcgctgg cgcgctacca gctcgcgtac gccgccccgg cggcagacgc tgcggtcgac
      721 cgtgggatcc gacaggtggt cagggcggtg ggtcacgcgt tcacggcggt cctcttcgtc
      781 gttcctgcgc tgctggcggt gctggtcggc gtggtcgggc ggctcgtggg gtccgatccg
      841 ctccaccgca cgacggggtg gagcccggtc ccctccgccg acggatcggg ggccgagcgc
      901 agccgggggc gcaccccccg gctggccctt gctgccacgg cgatcatctt gctggccgcg
      961 accacggtct gggtcgtccg ggaccgttcg agcgcgggga cggggaagac ggtgtcggcg
     1021 atcgtgccgc gggccgaggc gctccagggg caggaggaca tcgatgaggt cctcgaggag
     1081 gagggcgcgg tgttccgcag ctaccaaccc gacccggtcg tcggctggaa cctccccgaa
     1141 cggttcgacg ggtcggcggt cagcgtcgaa gaccatcgcc gcaccaccgt ccaagcagct
     1201 gatcggggac cgttggtctg gctcttcggc ggatcgacca tgttcggcag caaccagcgt
     1261 gacgaccgca cgatcgcctc gcgcttcgcg gcgatctcga cggcgaacgg aagaccggtg
     1321 cgggtggtca actggggtcg ccacgccttc accgcctacc agcaagcgca gctcctgcag
     1381 cacgagctgc gccgcgccga cgagctgccg gacctggtgg tgttctacga cggtttcaac
     1441 gacgtcgcgg tgggcgtggg gacgagcctc ctggaagggc cgagcaccga accgccccga
     1501 gccccctcca tcgctgaggg ctcgccggcc gagccctcac gtggacggcc tcggtggatg
     1561 acgcccgccc agcgctacga ccagatcgcc gccgactacc ggaaggcagg cgatctcgca
     1621 cggagggccg cggctgccca tgggatcgac gtggtccggg tgtggcagcc gagcgcgttc
     1681 acgcggcgcc ccctcacccg gggcgaacgg gtgcagctcg cccgcctcgg aatcgatgac
     1741 caggcgctcc agacgttgag gacggcgcac gaggaggtcg gcaggcggct tccaccgagc
     1801 gtgatcgatc tgtccggcgc attggacgac gcccccacgg tgttcgtcga tgaggtgcac
     1861 accaacgaga gaggcgcgga gctggtggcg gaggcgctcg agtcggccct cgccggccgg
     1921 gtacgagacc tcgagccctg accggtccct gtgctgcgcc ctccctgcgg tcagtgcggg
     1981 gccgagcggg tggcgaggag ccaatccgcg tgatcgccca cacgttcgct gaggatccgg
     2041 acctcggtct cctggtcgct cgactcgaac cgcaggagcc gatcgcctcg gtcgacgcgg
     2101 tgctcctcgt aggacgagtc cgcagggcgg ccgaaggcgg cgtgaagcca cacgaagagc
     2161 tcgtcgatcc cgccgccgac gaggtgctcg acgccgaggt cggcgaggtg cccgggatcg
     2221 atcgagcgga tcgagccttc cggccccacg tccagctgga gcggctgcac
//
LOCUS       DATMFS010000125         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_284094, whole genome shotgun sequence.
ACCESSION   DATMFS010000125 DATMFS010000000
VERSION     DATMFS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284094"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VNQ33_02320"
     CDS             complement(<1..395)
                     /locus_tag="VNQ33_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWJ96963.1"
                     /translation="MALAGIEKQFGKGSVMKMGEKGSMAIDTVPTGALALDLALGVGG
                     LPRGRVVEIYGPESSGKSTLAMHVVAEAQRNGGICAYVDAEHAMDPVYARAIGVDIDE
                     LLISQPDTGEQALEITDMLVRSGALDVVV"
     gene            complement(701..1510)
                     /locus_tag="VNQ33_02325"
     CDS             complement(701..1510)
                     /locus_tag="VNQ33_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWJ96964.1"
                     /translation="MNYPFLVLDFSVGSGEGEHVTSHLFMIMAVGAGIGFLGGLLGKG
                     GSALATPLLVAFGIPPVIALAAPLPATVPGTLVAADSYRRKGLIDRNVLVWSLLAGVP
                     ATVVGAIGSKFVDAHALILVTDAVIAVLGLRMVFGASHGDAAVADGPVDRRLIIGVAV
                     VTGLAAGLLANSGGFLLAPLFIAVLGMAIKPALGTSLAVAAVLAVPGTLVHLALGHLD
                     LVVVVAFAVGSVPLSRFGARVALRTDPSRLERGYGAVLALLGAGFLLAAFV"
     gene            1524..2447
                     /locus_tag="VNQ33_02330"
     CDS             1524..2447
                     /locus_tag="VNQ33_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ96965.1"
                     /translation="MDLRQASYVVAVVDHGTFTAAAASIPVSQPALSQAVSSLERELG
                     TELFHRIGRQVVLTAAGEAFVEPARRMLRDAEVARSVVEQVAGLAGGRLDVVALPSLV
                     VEPLVDLVGRFRKLHPQVLVRLTEPEDAAAVATLVRSGQCELGLGDQATDDRVLASDV
                     LGEQSLLAVLPPGTPLSADRDLPVARLARFPLITSPRGTSSRRLLANALRQADPDTDP
                     RIGVVTEHREAIVPLVMAGAGAAVLPEPLARAAAALGAVVAPLSPPLRRAVVLVRRVG
                     PLSPAARAFRDLALEHANVAPTAATDGLAAG"
     gene            2515..3045
                     /locus_tag="VNQ33_02335"
     CDS             2515..3045
                     /locus_tag="VNQ33_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ96966.1"
                     /translation="MADPAPSTSRPLAAAMTDDGGGLPLEDLEALYREHYEPVVRLAH
                     VLLGDRGRAEELAQDAFVRIAPRLGAIENPAGYLRTVLVNLCRDDGRRAARAKDHPAP
                     VARVAPEPPVPPTSTAVWAALQSLPERQRVAVTLRYYLDLPDDEIAEIIGSRPATVRS
                     LVHRALATLKEAVPHA"
     gene            3038..>3334
                     /locus_tag="VNQ33_02340"
     CDS             3038..>3334
                     /locus_tag="VNQ33_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96967.1"
                     /translation="MRDPVNPADPDRTWFRSHIEPVLDATPADDGWPRIAARIDGQPI
                     PEHRAPTRHRARRAAVAAAAAVLLAGLAMVVTAGRGDHDASRLTTAPPAPTGWYV"
BASE COUNT          490 a         1269 c         1155 g          420 t
ORIGIN      
        1 atcaccacga cgtcgagcgc accggagcgc accagcatgt cggtgatctc gagcgcctgc
       61 tcccctgtat cgggctgcga gatgagcagc tcgtcgatgt cgaccccgat ggcccgggcg
      121 tagaccgggt ccatggcgtg ctcggcatcg acgtaggcgc agatgccgcc gttgcgctgc
      181 gcctcggcca ccacgtgcat ggcgagggtg gacttgcccg aggactccgg gccgtagatc
      241 tccacgacac ggccacgggg cagaccgccc acacccaggg ccagatcgag cgccagcgca
      301 cccgtgggca ccgtgtcgat cgccatcgac cccttctcgc ccatcttcat gaccgagccc
      361 ttgccgaact gcttctcgat gccggccaga gccatgtcga gtgccttgtc tcgctccact
      421 gcgttctcct tctcgtgaac cacgacggtg ttggtgtggc tgcggcgaac gcagcgatcc
      481 gatgcgatgg accctacgga gggggtctga cagcgaagcc cgccggagcg gtctgagctg
      541 gtcgctccgg cgcaccggaa cgacatgcat ggtatttcga acacctgttc ggagcaagga
      601 ccaagcggag aaaattctcc agcccgcccg gcaacggtcg ccggggcggg tcgccggcct
      661 ctcgaccggc gggaggccgc caccgcagcc gccgggcggt tcagacgaag gcagcgagga
      721 ggaagccggc gcccagcaag gcgagcaccg cgccgtaccc gcgctcgagg cgggaggggt
      781 cggtgcgcag ggcgacccgg gcaccgaacc gggagagcgg caccgacccg acggcgaacg
      841 cgaccaccac cacgaggtcg aggtggccga gggccaggtg gaccaaggtg ccgggcacgg
      901 ccagcaccgc ggccacggcg agcgacgtgc cgagggccgg cttgatggcc atgccgagca
      961 cggcgatgaa gagcggagcg aggaggaagc cgccgctgtt ggcgaggagc ccagccgcga
     1021 gccccgtgac gacggcgacg ccgatgatga ggcggcggtc caccggccca tcggccaccg
     1081 ccgcgtcacc gtggctggcg ccgaacacca tgcgcaggcc gagcacggcg atgacggcgt
     1141 cggtcacgag gatgagggcg tgggcgtcga cgaacttcga gccgatggca ccgacgaccg
     1201 tggccggcac accggcgagg agcgaccaca ccagcacgtt gcggtcgatg agccccttgc
     1261 gccggtagct gtcggcggcc accagcgttc cgggcaccgt ggccgggagc ggggcggcga
     1321 gagcgatgac cggggggatc ccgaaggcga cgagcagcgg cgtggcgagc gctgatccgc
     1381 ccttgccgag cagccctccc aagaacccga tgccggcgcc gacggccatg atcatgaaga
     1441 ggtgggaggt cacgtgttca ccctcccccg acccaactga gaagtccaag accaagaacg
     1501 gatagttcac ataagctgaa cctatggatc ttcgccaggc gtcgtacgtg gtcgcagtgg
     1561 tcgaccacgg cacgttcacg gccgccgccg cctcgatccc cgtcagccaa ccggcgctct
     1621 cccaggcggt ctcgtcgctc gagcgggagc tgggcaccga gctgttccac cgcatcggcc
     1681 gccaggtggt gctcaccgcg gccggcgaag cgttcgtgga gccggcccgt cgcatgctgc
     1741 gcgatgccga agtggcgcgg tcggtggtgg agcaggtggc gggactcgcg ggcggccgcc
     1801 tcgacgtggt cgcgctgccg agcctggtgg tcgagccgct cgtcgacctc gtgggccggt
     1861 tccgcaagct gcacccccag gtgctggtcc gcctcaccga gcctgaggac gcggccgccg
     1921 tcgccaccct cgtccgctcg ggccagtgcg agctcggcct cggcgatcag gccaccgatg
     1981 accgcgtgct cgccagcgat gtgctcggcg agcagtcgct cctcgccgtg ctcccaccgg
     2041 ggacgccgct cagcgccgac cgggatctgc cggtggcccg cctggcccgg ttcccgctca
     2101 tcaccagccc tcgggggacc tcgagccggc ggctgctcgc caacgcgctg cgccaagccg
     2161 atcccgacac cgatccgcgc atcggcgtcg tcaccgagca ccgcgaggcg atcgtcccgc
     2221 tggtgatggc gggtgccggt gccgccgtcc tccccgagcc gctcgctcgc gccgcggccg
     2281 cgctcggggc cgtggtggct cccctctcgc ccccgttgcg ccgggcggtg gtcctcgtgc
     2341 ggcgcgtcgg cccgctctcc cccgccgccc gtgcgttccg ggacctcgcc ctcgagcacg
     2401 cgaacgtcgc gcccaccgcc gcgaccgacg gcctcgccgc cggttgagcg accggacccg
     2461 agcgatgcac gcaacagaac cggcggaccg ttcgtcagac caggtgtgca cgacgtggcg
     2521 gatcccgcac cctcgacgag ccgaccgctg gccgcggcga tgaccgacga cggcggcggc
     2581 ctgccgctcg aggacctcga ggccctctac cgcgagcact acgagcccgt cgtccgcctc
     2641 gcccacgtcc tcctcggcga ccggggtcga gcggaagagc tggcccagga cgcgttcgtc
     2701 cgcatcgccc cgaggctcgg cgccatcgag aaccccgccg gctacttgcg caccgtgctc
     2761 gtgaacctgt gccgcgacga cgggcgccga gccgccaggg cgaaggacca tcccgctccg
     2821 gtggcccgcg tggcacccga gccacccgtg ccgccgacct cgaccgcggt gtgggccgcg
     2881 ctccaatccc tgcccgagcg ccagcgcgtc gccgtcaccc tccgctacta cctcgacctg
     2941 cccgacgacg agatcgccga gatcatcggc tcgcgacccg ccaccgtgcg ctccctcgtc
     3001 caccgcgccc tcgccaccct caaggaggcg gtcccccatg cgtgatcccg tcaaccccgc
     3061 cgaccccgac cgcacctggt tccgttcgca catcgaaccg gtgctcgacg cgaccccggc
     3121 cgatgacggg tggccccgca tcgcggcccg catcgacggg cagccgatcc cggagcatcg
     3181 agcgccgacg aggcaccgtg cccggcgggc agccgtcgcc gcggcagccg cggtcctcct
     3241 cgccgggctc gccatggtgg tcacggccgg tcgcggcgac cacgacgcga gccggctcac
     3301 gaccgcgccg ccggcgccta ccggctggta cgtg
//
LOCUS       DATMFS010000126         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28587, whole genome shotgun sequence.
ACCESSION   DATMFS010000126 DATMFS010000000
VERSION     DATMFS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28587"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNQ33_02345"
     CDS             <1..488
                     /locus_tag="VNQ33_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96968.1"
                     /translation="FVLAVDASRAAIAVRLTADCPLTDPYWVDATVEPVAAGRADLAH
                     NAGPAYVRGFDVEAVATAALRQAAESATAPHERVHVTPWLYEQGDRFRIEEVAWPDDG
                     SDLRLTVDTDDDLAVVRAVCDRVPDPVTASWQDFVAVLRDDPALAARNAHVRQKDVGE
                     G"
     gene            485..1516
                     /locus_tag="VNQ33_02350"
     CDS             485..1516
                     /locus_tag="VNQ33_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96969.1"
                     /translation="MRSAAPHVVLLCDATRASGAGHLSRCVALGEAVIDTGGTATVAG
                     RIDPAFARLTDGSGVDLDDLVSPWATPEGEAARLAVIQRQAPATVVVDSYDLRARLLA
                     GLASVTRTVAIDDFARLDAYPCDVVVNFTVGASELSYPAGPELLLGPQWLLARRGLRR
                     ARAHRQETDAGAPVRRVLVAIGGVDRAGATARVVDVLLAADDELEIDVVAGRAFPGHD
                     EVRSRLDRLGNRSGVTISAPSLAPHLTRADLVVCGGGLTKYEAAYLGVPTVVIDQTAD
                     QAWESDRFTARGLAVRAGRIDDPGGDLTEPIVGLLRDGEQRRALAAASRAAFPSDPTA
                     AAARRILAR"
     gene            1583..2266
                     /locus_tag="VNQ33_02355"
     CDS             1583..2266
                     /locus_tag="VNQ33_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ96970.1"
                     /translation="MEHRQDLDALDRHYTAALEAHGDAPGAVQWSSQETQERRMEVLA
                     EIVGPDELRTASILDFGCGTGHLLTWLRRTHRYEGSYTGYDIAGAMIQAARALHASDP
                     GASFERRDIGTEGVGGDFDLVLVSGTFNNVVSDNWRFLTDHLRILFPHARRGLSFNCL
                     SSYVDYTTEGLAHRRPEDVFSFVKAELTPRVALRHDYHVKDDVIPFEFTVYAYASEHD
                     PVADRGTAP"
     gene            2263..2991
                     /locus_tag="VNQ33_02360"
     CDS             2263..2991
                     /locus_tag="VNQ33_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HWJ96971.1"
                     /translation="MTTAVITQPTYLPWLGAFEAIARADVYVVLDTVQFQRRSWHSRN
                     RLKAGNGEPFWLTVPVQRAPQQTPLAEILVADPAIDDWAAKHRRSIGLALAATPHAAD
                     AASCVQAALDDPPVRLVDLNVAIIEATAGALGLRPTWVRASELGVTGARSSLLTAICR
                     EVGADRYYSAAGSRGYMEEEQDVWDRSGLEVVFQEWEHPTYDQPGSAPFVSHLAALDP
                     IANLGFGGAAAAIGSTAPDQEPCR"
     gene            2988..>3715
                     /locus_tag="VNQ33_02365"
     CDS             2988..>3715
                     /locus_tag="VNQ33_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HWJ96972.1"
                     /translation="MTVTVGARRIGPGHPTFLVAEISANHNHDLDRALALVDAAADAG
                     VDAVKLQTYTADSMTLDVDRPEFRIGGGTAWDGDTLHRLYGEAATPYAWHAPLRDRAL
                     DRGLEFFSTPFDGDAVDLLDDLGVDVHKVASFEVTDTGLLERIGATGKPVIMSTGMAT
                     ETEIARALDALDRAGSGPVILLKCISAYPAPVEEMNLRAIGALATRFDRITGLSDHTL
                     GTEVAVAAVALGAHVIEKHITLARA"
BASE COUNT          527 a         1421 c         1246 g          521 t
ORIGIN      
        1 ggttcgtcct cgcggtcgac gccagtcgcg ccgcgatcgc ggtccgcctc accgccgact
       61 gcccgctcac cgacccgtac tgggtcgacg ccaccgtcga acccgtcgcg gcgggccggg
      121 ccgatctcgc ccacaacgca ggaccggcct acgtgcgggg cttcgatgtc gaagcggtcg
      181 ccaccgccgc gctgcgccag gccgccgagt cggccaccgc gccgcacgag cgcgtccacg
      241 tcaccccctg gctgtacgag cagggcgacc ggttccggat cgaggaggtc gcctggcccg
      301 acgatggctc ggatctccgc ctcaccgtcg acaccgacga cgacctcgcc gtcgtccggg
      361 ccgtgtgcga ccgggttccc gatccggtca cggccagctg gcaggacttc gtggccgtct
      421 tgcgagacga tcctgcgctg gccgcccgca acgcccacgt tcgccagaag gacgtcggcg
      481 agggatgaga tccgcggcgc cgcacgtcgt cttgctgtgc gacgccaccc gagcgtccgg
      541 tgccggccac ctcagccgct gcgtggccct cggcgaagcg gtcatcgaca ccggcggcac
      601 cgccaccgtg gccggccgca tcgacccggc cttcgcccgc ctcaccgacg gctccggggt
      661 cgatctcgat gatctcgtca gcccctgggc cacgcccgaa ggcgaagcgg cccggttggc
      721 ggtcatccag cgccaggctc ccgccacggt cgtcgtcgac agctacgacc tgcgggcacg
      781 cctcttggcc gggctggcct ccgtcacccg gaccgtggcg atcgacgact tcgcccggct
      841 cgacgcctac ccctgcgatg tggtcgtgaa cttcaccgtc ggcgcgtccg agctctcgta
      901 ccctgctggt ccggagctgc tcctcggccc ccagtggctg ctcgcccgcc gcggcctccg
      961 ccgtgcccga gcccaccgcc aggagacgga tgccggtgct cccgtgcgcc gggtcctcgt
     1021 cgccatcgga ggcgtcgacc gagcgggagc gacggcacgc gtcgtcgacg tcctcctcgc
     1081 ggcggacgac gagctggaga tcgacgtggt cgcaggccgc gcctttccgg gccacgacga
     1141 ggtccggtcc cggctggatc gcctcgggaa ccgctctggc gtcaccatct ccgcgccatc
     1201 cctcgccccg cacctcaccc gtgccgatct cgtggtgtgc ggaggcggcc tcacgaagta
     1261 cgaggcggcg tacctcggcg tgccgacggt cgtcatcgac cagaccgccg accaggcatg
     1321 ggagtccgac cggttcacgg cgaggggcct cgccgtccga gcgggacgca tcgacgatcc
     1381 tgggggcgac ctgaccgagc cgatcgtcgg gctcctccga gatggcgagc agcgtcgagc
     1441 gctggccgcg gcaagtcgag cggcgttccc gagcgacccc accgcggcgg ccgcgcgccg
     1501 gatcctcgcc cgctgaccgg gccacggcgc ggcgcggccc caaagccgag gaagcgccgc
     1561 ggtcgactgc aagactgcgg gcatggagca ccggcaggac ctcgacgcgc tcgaccggca
     1621 ctacaccgct gcgctcgagg cccacggcga tgctcctggt gccgtccagt ggtcgagcca
     1681 ggagacccag gagcgccgga tggaggtcct ggcggagatc gtgggaccgg acgagctgcg
     1741 cactgcgtcg atcctcgact tcgggtgcgg aaccggtcac ctgctgacct ggctgcgccg
     1801 gacgcaccgc tacgagggct cctacacggg ttacgacatc gccggcgcca tgatccaggc
     1861 ggctcgagcg ctccacgcca gcgatcccgg cgcgtccttc gagcggcgcg acatcgggac
     1921 cgagggcgtc ggaggcgact tcgacctggt gctggtcagc ggcacgttca acaacgtcgt
     1981 gtcggacaac tggaggttcc tgaccgacca cctgcggatc ctcttccccc acgcccggcg
     2041 cggactgtcg ttcaactgcc tcagctccta cgtcgactac accaccgaag gcctcgccca
     2101 tcgccggccc gaggacgtgt tctcgttcgt caaggcggag ctgacgcccc gcgtggcgct
     2161 gcgccacgac taccacgtca aagacgacgt gatcccgttc gagttcacgg tctacgcgta
     2221 cgcgtccgag cacgatccgg tggcggaccg ggggaccgca ccgtgaccac cgccgtcatc
     2281 acccagccca cctaccttcc ctggctgggc gccttcgagg cgatcgcgcg agcagacgtg
     2341 tacgtcgtcc tcgacaccgt ccagttccag cggcggagct ggcacagccg gaaccggctg
     2401 aaggccggca acggcgagcc cttctggctg acggtgcccg tccagcgagc gccccagcag
     2461 acacccctgg ccgagatcct cgtcgcggac cccgccatcg acgactgggc ggcgaagcac
     2521 cggcgcagca tcggcctcgc gctcgccgcc acgccgcacg cagccgacgc ggcgtcgtgc
     2581 gtgcaggcgg ccctcgacga tccgcccgtc cgcctcgtcg acctcaacgt cgcgatcatc
     2641 gaggccaccg ccggcgcgct gggcctgcgc cccacgtggg tgcgcgcttc cgagctcggc
     2701 gtgacgggtg ctcgcagctc gctgctcacc gcgatctgcc gggaggtcgg tgccgatcgc
     2761 tactactccg cggccgggag ccgcggctac atggaggagg agcaggacgt gtgggaccga
     2821 tcagggctcg aggtcgtgtt ccaggagtgg gagcacccca cctacgacca gcccggctcg
     2881 gcgccgttcg tctcgcacct cgccgcgctg gatccgatcg ccaacctcgg cttcggtggc
     2941 gctgcggccg ccatcggatc cacggcaccg gatcaggagc cgtgccggtg acggtcaccg
     3001 tcggcgctcg acgcatcggt ccgggccacc ccacgttcct cgtcgcggag atctcggcca
     3061 accacaacca cgatctggac cgggccctgg ccctggtcga cgcggccgcc gacgccggcg
     3121 tggatgccgt gaagctgcag acgtacacgg cggactcgat gacgctcgat gtggaccgcc
     3181 ccgagttccg catcggcggg ggcaccgcct gggatggcga caccctccac cgcctctacg
     3241 gcgaggccgc cacgccctat gcgtggcatg cgccgctacg ggaccgtgcg ctcgatcgag
     3301 gtctcgagtt cttctccacg cccttcgacg gcgacgccgt cgacctgctc gatgacctgg
     3361 gcgtcgatgt ccacaaggtg gcgagcttcg aggtgaccga caccggcctg ctcgagcgca
     3421 tcggcgccac cggcaagccg gtgatcatgt ccaccggcat ggccacggag accgagatcg
     3481 cacgggcgct cgacgccctc gatcgagccg gcagcggacc ggtgatcctg ttgaagtgca
     3541 tcagcgccta cccggccccc gtcgaggaga tgaacctgcg agccatcggc gcccttgcca
     3601 cccggttcga ccggatcacg ggcctctccg accacaccct cggcaccgag gtggcggtgg
     3661 cggcggtggc gctcggtgcc cacgtgatcg aaaagcacat caccctggcg cgagc
//
LOCUS       DATMFS010000127         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_167361, whole genome shotgun sequence.
ACCESSION   DATMFS010000127 DATMFS010000000
VERSION     DATMFS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167361"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VNQ33_02370"
     CDS             complement(<1..689)
                     /locus_tag="VNQ33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWJ96973.1"
                     /translation="MSSPLDGITVLDLASVGPAARTSRWLADFGARVVKVGPTPKRSG
                     VQIVPPFYAYGGHRGMERVLLDLKADAGREAFLKLAATADVVIESFRPGVVDRLGIGF
                     DAVSAVNPGVVYCSTSGYGQTGPKSQWAGHDINYLAVSGYLDCSGRNAEGGPALPGAT
                     VADSAAGGMHAVMSVLAALVARNATGEGQHLDVAVADGAVALMSLSVDEYLATGTVPG
                     PGHNILTGRYA"
     gene            complement(694..1914)
                     /locus_tag="VNQ33_02375"
     CDS             complement(694..1914)
                     /locus_tag="VNQ33_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ96974.1"
                     /translation="MDFEFSPEQQTFIEQVEAFLDENNDPDVFDVTRENMAQIVDTPK
                     RRAFMAKLGEMGWLGMTWPKEYGGSEGEGVYEYLLNERLAGRGGPQIGKGVGIIGKTL
                     IAHGNDFLKETFLPKILRNEVEFAVGYSEPNAGSDAASMRLKGERGERDGKTGWILNG
                     QKTWTTSAHFAEWYWLGVRTDPNNKHRGITLMLVPIDQPGITINKIDTMGGVPMGDER
                     TNEVFVDDVFVPDEYVVGEVNSGFQYISQALDLERFTMFTYAPIKERLDVLTEHVTTE
                     TVDGEPIKDDPLVRQRIAQLATEAEVARVMGLRVVSESMKADETPGTPPPTVESSQYK
                     LFATEFSKRLADASMDLGAPGTQLRVKTAAAPMAGRAESTYRYTVIDTIGGGASEIQK
                     NVIARRGLGLPKNF"
BASE COUNT          295 a          733 c          789 g          349 t
ORIGIN      
        1 caggcgtacc ggccggtgag gatgttgtgg ccggggccgg ggacggtgcc ggtggcgagg
       61 tactcgtcga cggagagcga catgagggcg acggcgccgt cggccaccgc cacgtcgagg
      121 tgctggccct cgccggtggc gttgcgggcc accagggcgg cgaggaccga catcaccgcg
      181 tgcatgccgc ccgccgcgct gtcggccacc gtggcgccgg gcagcgcggg accgccctcg
      241 gcgttgcggc ccgagcagtc gaggtagccg ctgacggcga ggtagttgat gtcgtggccg
      301 gcccactggc tcttcggccc ggtctgcccg tagccgctgg tggagcagta gacgacgccg
      361 gggttcacgg ccgagacggc gtcgaagccg atgccgaggc ggtcgaccac gccgggacgg
      421 aagctctcga tcaccacgtc ggcggtggcc gccagcttca ggaacgcctc gcggccggca
      481 tcggccttga ggtcgagcag cacccgctcc atgccgcggt ggccgccgta cgcgtagaag
      541 ggcgggacga tctgcacgcc cgaccgcttc ggcgtcggcc cgaccttgac gacccgcgcg
      601 ccgaagtcgg ccagccagcg cgaggtgcgc gccgccgggc cgacggaggc gaggtcgagg
      661 acggtgatgc cgtcgagcgg ggaggacacg gggtcagaag ttcttcggga gcccgaggcc
      721 gcggcgggcg atcacgttct tctggatctc gctggccccg ccgccgatcg tgtcgatgac
      781 cgtgtaccgg taggtgctct cggcccggcc cgccatcggg gccgccgcgg tcttgacccg
      841 cagctgcgtg ccgggggcgc cgaggtccat cgaggcatca gccagccgct tggagaactc
      901 ggtggcgaac agcttgtact gcgacgactc gacggtgggc gggggcgtcc ccggcgtctc
      961 gtcggccttc atggactcgc tgaccacccg caggcccatg acccgggcga cctcggcctc
     1021 ggtggcgagc tgggcgatgc gctggcggac gagcgggtcg tctttgatcg gctcgccgtc
     1081 gacggtctcg gtggtcacgt gctcggtcag cacgtcgagg cgctctttga tgggcgcgta
     1141 cgtgaacatc gtgaagcgct cgaggtcgag cgcctgcgag atgtactgga agccggagtt
     1201 gacctcgccc accacgtact cgtcgggcac gaacacgtcg tcgacgaaca cctcgttggt
     1261 gcgctcgtcg cccatcggga cgccgcccat ggtgtcgatc ttgttgatgg tgatgccggg
     1321 ctggtcgatc ggcacgagca tcaaggtgat gccccggtgc ttgttgttcg ggtcggtgcg
     1381 tacgcccagc cagtaccact cggcgaagtg ggccgaggtg gtccacgtct tctgcccgtt
     1441 caggatccag ccggtcttgc cgtcgcgctc gccccgctcg cccttgaggc gcatcgacgc
     1501 cgcatccgaa ccggcgttgg gctcggagta ccccaccgcg aactccacct cgttgcggag
     1561 gatcttcggg aggaaggtct ccttgaggaa gtcgttgccg tgggcgatga gggtcttgcc
     1621 gatgatgccg acgcccttgc cgatctgcgg gccgccccgg ccggcgaggc gctcgttgag
     1681 caggtactcg tagacgccct cgccctcgga gccgccgtac tccttcggcc aggtcatgcc
     1741 cagccagccc atctcgccga gcttggccat gaacgcccgc cgcttgggcg tgtcgacgat
     1801 ctgcgccatg ttctcccggg tgacgtcgaa gacgtccggg tcgttgttct cgtcgaggaa
     1861 cgcctcgacc tgttcgatga acgtctgctg ctcgggtgag aactcgaagt ccatgggtgc
     1921 tcctgcgacc agatgcggtg gggtgccgcg agaatctgac gggtcgtcag atccgcgcag
     1981 gatcatacgg ccggccctcc aggccgacaa acggcgcgac cgctcaccgg ccccgggcgt
     2041 agcgtccacg gcgatgaccg cctcgtgctc ctccgacgat ccgctcccgg cgggagacct
     2101 cggcacctgg ctcgacgcgc tgccgcggac catcgccggc gacgaggacg ccgaggtgcc
     2161 gtgcgg
//
LOCUS       DATMFS010000128         4002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_121957, whole genome shotgun sequence.
ACCESSION   DATMFS010000128 DATMFS010000000
VERSION     DATMFS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121957"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /gene="groL"
                     /locus_tag="VNQ33_02380"
     CDS             complement(<1..1429)
                     /gene="groL"
                     /locus_tag="VNQ33_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224954.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWJ96975.1"
                     /translation="MPKIIAFDEQARRALESGMNQLADAVAVTLGPKGRNVVLEKKWG
                     APTITKDGVSVAKDIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLARAMVTEG
                     LKNVAAGANPMDLKRGIEAAVEAAVGAIHALSVEVDDKEQIAKVASISANNDAEIGNA
                     IAEAMDKVGRDGVITVEESNTFGIETDFVEGMRFDKGYISPYFVTDPERMEAVLDDAY
                     VLLVSSKISAVRDLVPVLEKVMQGGKPLVIIAEDIEGEALSTLVVNKIRGTFRSVAVK
                     APGFGERRKAMLQDIALLTGAQVISEDVGLKLDATTLDLLGTAKKVIITKDETTIVEG
                     GGSDEDIAGRVNQIKAEIDNTDSDYDREKLQERLAKLSGGVAVLKVGAATETELKEKK
                     HRIEDAVSTAKAAIEEGVVPGGGVALIRAQAAINEAAEKLEGDIATGARLVARAVEAP
                     LKQIALNAGLEGGVVVSKVKELTGTQ"
     gene            complement(1937..2215)
                     /locus_tag="VNQ33_02385"
     CDS             complement(1937..2215)
                     /locus_tag="VNQ33_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWJ96976.1"
                     /translation="MSVTVRVPTTLRTLTGGASEVALEGATVGAVLDALEDAHPGFKS
                     RLLDDQGGLRRFVNLYVADDDVRFRDGLDTPVADGETVSIIPAVAGGC"
     gene            2477..3385
                     /locus_tag="VNQ33_02390"
     CDS             2477..3385
                     /locus_tag="VNQ33_02390"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HWJ96977.1"
                     /translation="MTTSSGQRGVRSHLEHAFDLATVLSEKAATGITVSACIPARNED
                     ATVGFVVAAIRNALVDGALASSGTPLVDEIVVVDDHSTDDTAAVARAAGARVVDAASV
                     LAHHGEGHGKGEALWKSVHESTGDIVVWVDADIVDFDPDFIVGMVGALVCDTEVDFVK
                     GHYHRPEANGVGGGRVTELLARPLLSLYFPELADIVQPLAGEYAGRRTLLERLPFVVG
                     YGVDVALLIDAVRVAGIERIAQVDLGVRHHRNRSLDELGPQALAVGQAVLDRAGVRPD
                     GTAILHRPGREPLPVPMHERPPLNTL"
     gene            complement(3507..>4002)
                     /locus_tag="VNQ33_02395"
     CDS             complement(3507..>4002)
                     /locus_tag="VNQ33_02395"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWJ96978.1"
                     /translation="GGSATMGAHASRRPTMLADQIQTDLTAAMKERDELKLSVLRMAL
                     AAVKEAKVSGDEVRDLSDDDVIALLSKEAKRREEAAEAFAAGGREESAARELAERDVL
                     AAYLPAPASDEEIEALVAEALAEGGFSEPSQMGLAMKAAMAKVGGKADGKKVSALVKA
                     RLAG"
BASE COUNT          528 a         1421 c         1418 g          635 t
ORIGIN      
        1 cctgggtgcc ggtgagctcc ttgaccttgg agaccaccac gccgccctcg aggccggcgt
       61 tgagggcgat ctgcttgagc ggggcttcga cggcacgggc cacgaggcgg gcaccggtgg
      121 cgatgtcgcc ctcgagcttc tcggcagcct cgttgatggc ggcctgcgca cggatgagcg
      181 ccacgccgcc gccagggacg acgccctctt cgatggcggc cttggcggtg gagacggcgt
      241 cttcgatgcg gtgcttcttc tccttgagct ccgtctcggt ggcggcgccg accttgagga
      301 cggcgacgcc gcccgacagc ttggcgaggc gctcctggag cttctcgcgg tcgtagtcgg
      361 agtcggtgtt gtcgatctcg gccttgatct ggttcacccg gccggcgatg tcctcgtcgg
      421 agccgccgcc ctcaacgatg gtggtctcgt ccttggtgat gatgaccttc ttggcggtgc
      481 cgaggaggtc gagcgtggtg gcgtcgagct tgaggccgac gtcttcgctg atgacctggg
      541 cgccggtgag cagggcgatg tcctgcagca tggccttgcg gcgctcgccg aagccggggg
      601 ccttgacggc gaccgagcgg aacgtgccgc ggatcttgtt caccacgagg gtggagaggg
      661 cctcgccctc gatgtcctcg gcgatgatca cgaggggctt gccgccctgc atgaccttct
      721 cgagcaccgg cacgaggtcg cggaccgcgg agatcttcga ggacacgagc agcacgtagg
      781 cgtcgtcgag gacggcctcc atgcgctccg ggtcggtcac gaagtacggc gagatgtagc
      841 ccttgtcgaa gcgcatgccc tcgacgaagt cggtctcgat gccgaacgtg ttcgactcct
      901 cgacggtgat gacgccgtcg cggccgacct tgtccatcgc ctcggcgatg gcgttgccga
      961 tctccgcgtc gttgttggcg gagatggacg cgaccttggc gatctgctcc ttgtcgtcga
     1021 cctcgaccga cagcgcgtgg atcgcaccga cggccgcttc gacagcggcc tcgatgcccc
     1081 gcttgaggtc catcgggttg gcgccggcgg ccacgttctt gaggccctcg gtcaccatgg
     1141 cccgagcgag cacggtggcg gtggtggtgc cgtcaccggc gacatcgtcg gtcttcttgg
     1201 cgacctcctt gaccagctcg gcgccgatct tctcgtaggg gtcctcgagc tcgatgtcct
     1261 tggcgacgga gacgccgtcc ttggtgatgg tgggggcgcc ccacttcttc tcgagcacga
     1321 cgttgcggcc cttcgggccg agggtgacgg cgacggcgtc ggcgagctgg ttcatgccgg
     1381 attcgagggc acggcgggcc tgctcgtcga aagcgatgat cttgggcatc cctgggttct
     1441 cctggagacg gatggcgttt cgctgggggc tccggtcgga gccgctagca ctcccctaga
     1501 gagagtgcta acagccaacc acttccgagc gtcgttagca accgcgggcc gagactgcta
     1561 accgcaaccg gcgctgctcg ccgatccggc cacgctccgc ggcgcgaacg agccgttcga
     1621 cccatgtcgt ggccacgcgc tgcaccgatg gatagggcgt cgatccgctt cgacggatcg
     1681 acgtgggcgg gcgggctgcc aggagttgga gggagactgc tggtggcccg ccgccaacca
     1741 ccgagacgtc gccggtggga cgaccggtgg gaccacgcga cgcccgtcag agacgagccg
     1801 caccgacccc cgccggccgc gtctgcccga aaccgggcag gtcagcgcgg gcagggcagg
     1861 gcgggcggcg acgagccgct cgggcgcggg gaggcaggac cgatgaccca cccagggtgg
     1921 gccgaccgtg acgagctcag cagccgccgg cgacggcggg gatgatgctg acggtctcgc
     1981 cgtcggcgac cggcgtgtcc agcccgtcgc gaaagcggac gtcgtcgtcg gccacgtaga
     2041 ggttcacgaa gcggcgcagg ccaccctggt cgtcgagcag gcgcgacttg aagccggggt
     2101 gggcgtcctc gagcgcatcg agcaccgcgc ccacggtggc gccctcgagc gcgacctccg
     2161 acgcgccgcc ggtgagggtg cgcagggtgg tgggaactcg gacggtgacg ctcatgggat
     2221 ggggaacctc ggctcgtact ccacccggcg accaccgggc cgcgggtgat cgtagtggta
     2281 ccccctggcc gttccaccat ggcccgcctg gcccgacgtc aggttgccgt ggggcggcct
     2341 tcacgatggc gcaacgctga tgaatcgtgc gttccttacg ctggggtacg agttgcaccg
     2401 gggaaggcgt ccaacggcct ggtaccgcca ggcgcccgtc ttgcccggga ggggttgaac
     2461 gatgacgatg acgcccatga ccacgtcgag cggccagcgc ggggttcgat cgcatctcga
     2521 gcatgcgttc gacctcgcca ccgtgctctc cgagaaggcg gcgaccggga tcaccgtgtc
     2581 ggcctgcatc ccggcccgca acgaggacgc caccgtcggc ttcgtcgtgg ccgccatccg
     2641 caacgcgctc gtcgacggtg ccctcgcctc ctccggcacc ccgctggtgg acgagatcgt
     2701 cgtggtcgac gaccactcca ccgatgacac ggccgccgtc gcccgagccg ccggggcccg
     2761 cgtggtcgac gccgcctccg tcctcgccca ccacggcgag ggccacggca agggcgaggc
     2821 gctgtggaag tcggtgcacg agtccaccgg cgacatcgtg gtgtgggtcg acgcggacat
     2881 cgtcgacttc gacccggact tcatcgtcgg catggtcggg gcgctggtgt gcgacacgga
     2941 ggtcgacttc gtgaagggcc actaccaccg gcccgaggcg aacggggtcg gcggcgggcg
     3001 cgtcaccgag ctgctcgccc ggccgctgct ctcgctgtac ttcccggagc tggccgacat
     3061 cgtccagccc ctggccggcg agtacgccgg acgccgcacg ctgctcgaac ggctgccgtt
     3121 cgtggtcggg tacggggtcg acgtcgccct gctgatcgat gccgtgcggg tggcggggat
     3181 cgagcgcatc gcccaggtcg acctcggggt gcgccaccac aggaaccgct cgctcgacga
     3241 gctcggaccg caggccctcg ccgtcggtca ggccgtcttg gaccgggccg gcgtccgccc
     3301 cgacggcacc gccatcctcc accgcccggg ccgcgagccg ctgcccgtcc cgatgcacga
     3361 gcgccccccg ctgaacaccc tctgacccga cgacggccac ggacccgccg tggtcgacca
     3421 cggacccgcc gtcgtcgggc cacgatgccg gcccggcccc gctccccggc ggcgtcgacc
     3481 cccggcgccc gcccgtcgcc ttcggttcag ccggcgaggc gggccttgac cagggcggac
     3541 accttcttgc cgtcggcctt accgcccacc ttggccatcg ccgccttcat ggccaggccc
     3601 atctgcgacg gctcgctgaa gccgccctcg gcgagcgcct cggccaccag ggcctcgatc
     3661 tcctcgtcgg acgccggcgc cggcaggtag gcggcgagca cgtcgcgctc ggccagctcc
     3721 cgggccgcgc tctcctcgcg cccgcccgcc gcgaacgcct cggcggcctc ctcgcggcgc
     3781 ttggcctcct tggacaagag cgcgatcacg tcgtcatcgc tcaggtcccg cacctcgtcg
     3841 cccgacacct tggcctcctt gacggccgcc agcgccatgc gcagcacgga gagcttgagc
     3901 tcgtcgcgct ccttcatggc agcggtgagg tcggtctgga tctggtcggc gagcatcgtc
     3961 ggtctcctcg aagcgtgagc gcccatcgtg gccgatcccc cc
//
LOCUS       DATMFS010000129         4805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_330850, whole genome shotgun sequence.
ACCESSION   DATMFS010000129 DATMFS010000000
VERSION     DATMFS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330850"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VNQ33_02400"
     CDS             <1..1117
                     /locus_tag="VNQ33_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96979.1"
                     /translation="TTLCAVRVVPGADGEGDEIAWINVGDSRVYLFRDDNLIQLSRDH
                     SLVEDLLRDGQLTEDEAKVHPKRNIITRALGIDVDVDVDGSTVIPFNGDRFLLCSDGL
                     FGEVTADQISSVLRRLAEPTEAAEELVRLANESGGRDNITVVVVEVVDDDGRAAAASA
                     ALAEEAARLKAREGEGGAIDGDDADTQPAAGADPYVPVFADRKSSLPDAPDDFTSDDR
                     DGDPFDVLGKTKRFTWRVLVFLIALLVVVAVVVGAILWKKDTTYYLAITGDDVVLYKG
                     DPEGFLTDEAKATSLKVPSDELRPTAIQAAEAHKTFTSMEDADEFLFGFRIPATSTST
                     STSTSTSTTSTTRPRTTTATAPPVTTTTPATSTTAAP"
     gene            1156..2442
                     /locus_tag="VNQ33_02405"
     CDS             1156..2442
                     /locus_tag="VNQ33_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWJ96980.1"
                     /translation="MLAQSRRRAELGLLILAIAITGGAYTLASLGQTSSVPANIGPFF
                     AVITALFFAAHLAIRRLAPEADGLLFPLVALLNGIGYVFIARLDPKLADNQATWTFVG
                     IAAFVLTLAFVRRVRDLERYRYTFLAIGLVLLVLPLVPGLGITINGAKIWVGVGPISF
                     QPGEFAKIAFALFFASYLVEKRELLSMATWPKFRPMLPDLKHLGPVLLAWGIAILVMT
                     AQKDLGSSLLLFALFMSMLWVATGRIAYPVSGAILFGTAAYGAWTQFAHVQRRVSQWM
                     DPWSDVGGQLSETWYALAWGGVAGTGIGLGRPNKIPYVESDFIFAAIGEELGLMGGTA
                     ILIAYLLIVGSGLRIAVQSEHSFDKLLAAGLTALIGMQAFVILAGVIRLLPLTGVVLP
                     FVSYGGSALLANYVLLALLLRISDNNSRARRTAGAA"
     gene            2442..3956
                     /locus_tag="VNQ33_02410"
     CDS             2442..3956
                     /locus_tag="VNQ33_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWJ96981.1"
                     /translation="MDRSIRRLGAFMMLLFCALFVQLNYIQVLRASDLNIKPGNNRPI
                     DQSFAKPRGTIATADGVLLARSVKSNDRFQYQREYPEGELYAALTGYFSYVYGATGLE
                     RAYNEELSGKTAKQQIKSVGDLFVDKDRTGNLTLSVRSDIQKVAKAALGDFKGSVVAL
                     DPRSGDVLAMYSNPSFDPNLIADHDVEDSQAAYNWLNATEGKPMLAKSYREIYPPGST
                     FKVVTGSTGVEKGGVTPTEPVYPSVSSITVRGTTRPLRNFDNEQCGGTLFVVLARSCN
                     TSFAQMGLDLGGDKMVEGAQAFGFNSTPPFDLPAVPSHFKTDFTRDEPALAQSSIGQN
                     ETAATPLQMALVAAGVANDGVIMEPHVVSEIRDGEGDQVSKHDPTQWKRAISPQTAQT
                     MRDGMLEVVRNGSATRLQIEGYDVGAKTGTAQFGPSEPLRSHAWIIAWAAVPGQAPSI
                     AVAVIVENQTGASETTGGRLAAPIAKQVIEQSFQPMPAPPAGLPGSTTSTTAAN"
     gene            4013..>4805
                     /locus_tag="VNQ33_02415"
     CDS             4013..>4805
                     /locus_tag="VNQ33_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ96982.1"
                     /translation="MSNREQVVLNGRYELHRRVGRGGMAEVYLARDRLLDRLVAIKIL
                     FPEFATDPSFVARFRREAQAAANLNHPNIVGVYDWGKERGTYYIVMEYVDGQTVSEIL
                     RNEGPIEPKRAAGIAADVAAALGFAHRKGVVHRDVKPGNVLITKAGEVKVADFGIARA
                     MTASSEENLTQTGSVMGTATYFSPEQAQGKPVDARSDLYSLGVVLYEMASGKPPFSAD
                     SPVAIAYKHVQEPIPSLAEKVPGIPRDYEAITMRALAKDPDDRYAD"
BASE COUNT          743 a         1815 c         1535 g          712 t
ORIGIN      
        1 caccacgctg tgcgccgtcc gggtcgtgcc cggtgccgac ggcgagggcg acgagatcgc
       61 ctggatcaac gtcggcgact cgcgggtgta cctgttccgc gacgacaacc tcatccagct
      121 cagccgcgac cacagcctgg tcgaggacct gctgcgcgac gggcagctga ccgaggacga
      181 ggccaaggtc caccccaagc gcaacatcat cacccgggcc ctcggcatcg acgtcgacgt
      241 cgacgtcgac ggcagcacgg tcatcccgtt caacggcgac cgcttcctgc tctgcagcga
      301 cgggttgttc ggcgaggtca cggccgacca gatctcgtcg gtcctgcgcc gcctggccga
      361 gcccacggag gccgccgagg agctggtgcg cctagccaac gagagcggcg gccgagacaa
      421 catcaccgtg gtcgtcgtgg aggtcgtcga cgacgacggc cgagcggccg ccgcgtcggc
      481 tgcgctggcc gaggaggcgg cgcgcctcaa ggcccgcgag ggcgaagggg gcgcgatcga
      541 cggcgacgat gccgataccc agcccgccgc cggggctgac ccgtacgtgc cggtgttcgc
      601 cgaccggaag agctcgctgc ccgacgcacc cgacgacttc acgtccgacg atcgtgacgg
      661 cgatcccttc gacgtgctcg gcaagaccaa gcggttcacg tggcgcgtcc tggtgttcct
      721 gatcgcgctg ctcgtcgtgg tcgccgtcgt ggtcggggcg atcctctgga agaaggacac
      781 cacctactac ctggcgatca ccggcgacga cgtggtgctc tacaaggggg acccggaagg
      841 gttcctcacc gatgaggcga aggccacgtc cctgaaggtg ccgagcgacg agctgcgccc
      901 cacggcgatc caagcggccg aggcccacaa gaccttcacg tcgatggagg acgcggacga
      961 gttcctgttc ggtttccgga tccccgcgac atccacgtcc acgtcgacct ccacctccac
     1021 ctccacgaca tccaccaccc gtccgcggac cacgaccgcc acggctccgc cggtcacgac
     1081 gaccactccc gccacctcga ccaccgcggc gccgtgagcg cggtcgtcca ggcccgtcga
     1141 cgggcggatc ggagcgtgct cgcgcagtcg cggcgtcgag cggagctcgg cctgctgatc
     1201 ctcgccatcg ccatcaccgg cggggcgtac acgctggcgt cgctgggcca gacctcgtcg
     1261 gtgcctgcca acatcggtcc gttcttcgcc gtcatcacgg cgctgttctt cgccgcccac
     1321 ctcgccatcc gccggctggc tcccgaagcc gacggcctcc tgttcccgct ggtggccctg
     1381 ctcaacggca tcggctacgt cttcatcgcc cgactcgacc cgaagctggc cgacaaccag
     1441 gccacctgga cgttcgtcgg catcgccgcc ttcgtgctga cgctggcgtt cgtccgccgg
     1501 gtgcgcgacc tcgagcgcta ccgctacacg ttcctcgcca tcggcctcgt cctcctcgtc
     1561 ctcccgctcg tccccgggct cggcatcacc atcaacggcg ccaagatctg ggtgggcgtc
     1621 gggcccatca gcttccagcc gggcgagttc gccaagatcg cgttcgccct gttcttcgcc
     1681 tcgtacctgg tggagaagcg cgagctgctg tcgatggcga cgtggccgaa gttccggccg
     1741 atgctgcccg acctcaagca cctgggcccg gtgctgctcg cctggggcat cgccatcctc
     1801 gtcatgaccg cgcagaagga cctgggttcg tcgctgctgc tgttcgcctt gttcatgtcg
     1861 atgctgtggg tcgccaccgg ccggatcgcc tacccggtga gcggggcgat cctcttcggc
     1921 accgccgcct acggcgcctg gacccagttc gcccacgtcc aacggcgcgt gtcgcagtgg
     1981 atggacccct ggtccgacgt cggcggccag ctgtcggaga cctggtacgc cctcgcctgg
     2041 ggcggcgtcg ccggcacggg catcggcctc ggccggccca acaagatccc ctacgtcgag
     2101 agcgacttca tcttcgcggc catcggcgag gagctcggcc tcatgggcgg caccgccatc
     2161 ctcatcgcct acctgctcat cgtcggctcc ggtctgcgca tcgccgtcca gtccgagcac
     2221 tcgttcgaca agctcctcgc cgccgggctc accgcgctca tcggcatgca ggcgttcgtg
     2281 atcctcgctg gtgtcatccg cctgctcccg ctcaccggtg tggtgctgcc gttcgtgtcc
     2341 tacggcggct cggcgctcct cgccaactac gtgctgctgg cgctgctgct gcgcatctcc
     2401 gacaacaact cgcgcgcccg ccgcaccgcc ggggcggcgt gatggaccgc tcgatccgcc
     2461 ggctcggcgc gttcatgatg ctgctgttct gcgcgctgtt cgtgcagctc aactacatcc
     2521 aggtgctccg cgccagcgac ctgaacatca agcccggcaa caaccgaccc atcgaccagt
     2581 cgttcgccaa gccccgcggc accatcgcca ccgccgacgg ggtgctgctc gcccggtcgg
     2641 tgaagagcaa cgaccgcttc cagtaccaac gggagtaccc ggagggcgag ctgtacgccg
     2701 ccctcaccgg gtacttcagc tacgtgtacg gggccaccgg cctggagcgg gcctacaacg
     2761 aggagctgtc gggcaagacg gccaagcagc agatcaagtc cgtcggcgac ctcttcgtcg
     2821 acaaggaccg caccgggaac ctcacgctct cggtccgctc cgacatccag aaggtggcca
     2881 aggcggccct gggcgacttc aagggctccg tggtggcgct cgacccgcgc tcgggcgacg
     2941 tcttggcgat gtacagcaac ccgtcgttcg acccgaacct catcgccgac cacgacgtcg
     3001 aggacagcca ggcggcgtac aactggctga acgcgaccga gggcaagccg atgctcgcca
     3061 agtcctaccg ggagatctac ccgcccggct cgaccttcaa ggtcgtgacc ggctccaccg
     3121 gcgtggagaa ggggggggtc acgcccaccg agccggtcta cccgtcggtc agctcgatca
     3181 cggtccgggg gacgacccgc ccgctgcgga acttcgacaa cgagcagtgc ggtggcaccc
     3241 tcttcgtggt cctcgcccgg tcctgcaaca cgtcgttcgc ccagatgggc ctggacctgg
     3301 gcggcgacaa gatggtcgag ggcgcccagg ccttcggctt caacagcacg ccgccgttcg
     3361 atctgccggc ggtcccgtcg cacttcaaga ccgacttcac ccgggacgag ccggcactgg
     3421 cgcagtcgtc catcggccag aacgagacgg cggccacgcc gctgcagatg gcactcgtcg
     3481 ccgccggtgt cgccaacgac ggcgtgatca tggagcccca cgtcgtgagc gagatccgcg
     3541 acggcgaggg cgaccaggtc tccaagcacg acccgaccca gtggaagcgg gccatcagcc
     3601 cccagaccgc ccagacgatg cgcgacggca tgctcgaggt cgttcgcaac ggcagcgcga
     3661 cccggctcca gatcgagggg tacgacgtcg gcgccaagac cggcaccgcc cagttcggcc
     3721 cgtcggagcc gctgcgctcc cacgcgtgga tcatcgcctg ggccgccgtg ccgggccagg
     3781 ccccgtcgat cgccgtcgcc gtcatcgtcg agaaccagac gggggcgagc gagaccaccg
     3841 gcgggcggct cgccgccccg atcgccaagc aggtgatcga gcagtccttc cagccgatgc
     3901 ccgccccgcc ggccggtctc cccggcagca ccaccagcac gacggcggcg aactgatgca
     3961 ccgcggcggt gcccccttcc ccccagagcc ctctcgtagg ctggtctgag ccatgtccaa
     4021 ccgtgaacag gtcgtcctca acgggcgcta cgaacttcac cggcgcgtcg ggcgcggcgg
     4081 catggccgag gtgtacctgg cccgcgaccg gctgctcgac cgcctggtcg ccatcaagat
     4141 cctcttcccg gagttcgcca ccgacccgtc gttcgtggcc cgcttccgcc gcgaggccca
     4201 ggccgccgcc aacctcaacc accccaacat cgtgggcgtc tacgactggg gcaaggagcg
     4261 cggcacctac tacatcgtca tggagtacgt cgacggccag accgtctcgg agatcctccg
     4321 caacgagggc cccatcgagc ccaagcgggc cgccggcatc gccgccgacg tggccgccgc
     4381 cttgggcttc gcccaccgca agggcgtggt ccaccgcgac gtgaagcccg gcaacgtgct
     4441 catcaccaag gccggcgagg tcaaggtcgc ggacttcggc atcgcccggg ccatgacggc
     4501 cagcagcgag gagaacctca cccagaccgg gtcggtcatg ggcaccgcca cctacttctc
     4561 gcccgagcag gcccagggca agcccgtgga cgcccgctcg gacctgtact cgctgggcgt
     4621 cgtgctctac gagatggcgt cgggcaagcc gccgttcagc gccgacagcc ccgtcgccat
     4681 cgcctacaag cacgtgcagg agcccatccc gtcgctcgct gagaaggtcc cggggatccc
     4741 ccgtgactac gaggccatca cgatgcgggc cctggccaag gaccccgacg accgctacgc
     4801 cgacg
//
LOCUS       DATMFS010000130         5343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_75131, whole genome shotgun sequence.
ACCESSION   DATMFS010000130 DATMFS010000000
VERSION     DATMFS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75131"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VNQ33_02420"
     CDS             complement(<1..529)
                     /locus_tag="VNQ33_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96983.1"
                     /translation="MAVPAPDLAALRELGERVRPLSAAAERVLPVPPAFAGLIPQGGL
                     VRGSTVVTSGSAASSLALALAGPATAAGSWCAVVGAAHLGLLAAAELGVALERTLLIA
                     DPGPQEWAGTVAALLDAVEVVVVQPTGSITPTVQRRLVARARDRGSVLLQAGGCSEVW
                     ASRPDLAVDSGASRWE"
     gene            747..1418
                     /locus_tag="VNQ33_02425"
     CDS             747..1418
                     /locus_tag="VNQ33_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96984.1"
                     /translation="MSMDDEARVVDAPDPGAPVDVLDGAPVDGGSAPAGHGAPPAPTG
                     GGAPDVGASPLPERKRRWVRRVGVALLVLLLAGLSGLQIQAARVRAQAEEAETRRQTA
                     EQDEETARIKLESVGDRVRLARSQEDAAQQELDRARTDMTAKGFEEASLASVQKATAE
                     KVTGLRAGVKKVARDIAEQDRLQPAAGACLFDMLRALDRVDTGARSGRSSEACKTVAA
                     SPGPA"
     gene            1382..2815
                     /locus_tag="VNQ33_02430"
     CDS             1382..2815
                     /locus_tag="VNQ33_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HWJ96985.1"
                     /translation="MQDRGRIAGAGVTRRARRVDRPPSPKRRRLRQVLTAIVVVAAVT
                     GVAVSVRQLLGFEDRKEVADAATVDAHEEEADLVDRAADLEALIERLDAATAEHLDTA
                     AGIDKAVSDIGALRTKVDSLTKQVAEKREARAARQQRLDVLLGCRNTLDAAIAQLAGG
                     HVAATDAVGVLDQGAKRCQAALEIAQGDTGAVHPYDFPDPSLLIVGDTYYAYATEGPG
                     GAIQVLSSKDLQNWSVRTRALANRPEWAKAGPIWAPSVHAIGGRYVLYYATRSKISSQ
                     QCISTAVSNDPAGPFIDLSPVPLICQDDLGGSIDPDVYLDQFGFAHLTWKSEGETVGG
                     RAKIWTQALSADGLAVNGFAKSMFEAEDGWEQKTVENPSMVLLDGQWVLFYSGGKWNS
                     EGYSVGYALCDGAIGPCHRPVNPRLHTSAGGFVGPGGADVFRSRGGRSLAVYAAWDKG
                     KVGNPNARRLHVAEVSFHDGAVHWKDL"
     gene            complement(2823..3251)
                     /locus_tag="VNQ33_02435"
     CDS             complement(2823..3251)
                     /locus_tag="VNQ33_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ96986.1"
                     /translation="MRRSLIAVTVAVSLGLGALLAACGSDGGGPEVKLSAAGERGRQV
                     AKEQGCISCHSVDGGKGTGPTWQGLAGSTVTLDDGTEIVADDTYLREAILQSRGQVVD
                     GYANVMPVYDGELSDAEVADLIAYLHDLAPDTDAGTATTN"
     gene            complement(3257..3715)
                     /locus_tag="VNQ33_02440"
     CDS             complement(3257..3715)
                     /locus_tag="VNQ33_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96987.1"
                     /translation="MGQSIVVNEKPSSNPGVIRFETNRAFTGMDHERYLAGQEIWGER
                     PPDELARRLLDKPSVAGVQINGNMVTVDLNKGFDSTGLLEVIENLYRFYPDTPDTPEA
                     AEAEPGVDLAAEDAAADAQADPEPAAPVEEPAAADDAEAPAAEADAAPEA"
     gene            3805..4929
                     /locus_tag="VNQ33_02445"
     CDS             3805..4929
                     /locus_tag="VNQ33_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ96988.1"
                     /translation="MEAQRRTVDVRYVALTTFVAVSVVIGVVGSIWAVGRLWRVVSYL
                     LIALFFAAVLTPAVDFLQYRARMRRGVATLLVFLAGLAVLSAMIYAFVRPIVDQGQKF
                     SDSLPEMIDDAQEGKGRLGELITKYDLEEKFNENREAIDKQLNNAGSKGITILGTVFS
                     GIVAAVTVMVLTILLLLAGPDLSQSFLNIIPEPRRDRVRRVASDASMAVTGYMYGNVV
                     ISILAGIAAYIFLRIAGIPYPEVLALWVAFADLIPLVGATLGAIPPILVAFLTSTPAG
                     IATIVFFVLYQQFENNVLQVTVMARTVNINPLGVLVSVLIGVEVFGLLGALLAIPVAG
                     VIQVVVRDLWDERAGRPKAEITLGTDEIPMSEIDPPASPA"
     gene            complement(4954..>5343)
                     /locus_tag="VNQ33_02450"
     CDS             complement(4954..>5343)
                     /locus_tag="VNQ33_02450"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HWJ96989.1"
                     /translation="YRSIYLEGHDGLEGRILFTNAEPGQPDASYVGLDDPVADQKRIT
                     ELVQQGYLVRTRADANGVDAETGDTTRLDAALASGAQWVSTDFPGPDGAEAQYGTDYT
                     AALPGFLAARCNPVTAPSACEDGAVEP"
BASE COUNT          752 a         1926 c         1929 g          736 t
ORIGIN      
        1 cctcccaccg cgacgcaccc gagtcgaccg cgaggtcggg tcgagacgcc cacacctccg
       61 agcagccccc cgcctgcaac agcaccgagc cccggtcccg cgcccgggcc accagacgcc
      121 gctggacggt gggcgtgatg gagcccgtgg gctgcaccac cacgacctcg accgcatcga
      181 gcagcgccgc caccgtgccc gcccactcct gggggccggg gtcggcgatg agcagggtcc
      241 gctcgagcgc cacccccagc tcggcggcgg cgagcagccc caggtgggcc gcgcccacca
      301 cggcgcacca cgacccggcc gcggtggcgg gaccggccag ggccagcgcc aggctggagg
      361 cagcgctgcc gctggtgacg accgtgctgc cccgcaccag cccgccctgg gggatcagcc
      421 cggcgaacgc cggcggcacc ggcagcaccc gctccgccgc cgcgctgagc ggccgcacgc
      481 gctcgcccag ttcgcggagg gcggcgaggt caggggcagg aacggccatg cccgccagta
      541 tcgaacacct gttcgatatt ggcaaccgcc ctgctcagag agggccacgg cgaacctggt
      601 ccaggaccga aacctcccac gagtgcgtgg agcgggaacc gatcgggctt cgagggcgtc
      661 catctgacgg agaggacgtg ccgcgtcctg gagagggtgg tggagacgcg aacatggtgg
      721 ttccttcagg cggacgaggc gtgggagtga gcatggacga cgaggcgcgg gtggtggacg
      781 cccccgatcc cggcgcgccc gtcgacgtgc tcgacggcgc gcccgtcgac ggcggcagcg
      841 ctcccgccgg ccatggcgct cccccggctc ccaccggtgg cggcgcaccc gatgtcggcg
      901 cctccccgct tccggagcgc aagcgcaggt gggtgcggcg ggtgggggtg gcgctcctcg
      961 tgctgctgct cgccgggttg agcgggctcc agatccaagc ggcgcgggtc cgggctcagg
     1021 ccgaggaggc cgagacccgg cggcagaccg ccgagcagga cgaggagacg gcccgcatca
     1081 agctggagtc ggtgggcgac cgggtgcgcc tggcccggtc gcaggaggac gcagcgcaac
     1141 aggagctgga ccgggcccgg acggacatga cggccaaggg gttcgaggag gcgtcgctcg
     1201 cgagcgtcca gaaggccacg gccgagaagg tcaccggtct gcgagccggg gtgaagaagg
     1261 tggcgcggga catcgccgag caggaccgcc tgcagccggc ggccggtgcg tgcctgttcg
     1321 acatgctccg tgccctggac cgcgtcgaca ccggcgcccg cagcggccgg tcctccgaag
     1381 cgtgcaagac cgtggccgca tcgccggggc cggcgtgacg cgccgggccc gccgggtcga
     1441 ccgaccgccg agcccgaagc ggcgccgcct gcgccaggtg ctcaccgcca tcgtggtggt
     1501 cgccgccgtc acgggcgtgg ccgtgtcggt ccgccagctc ctcggcttcg aggaccgcaa
     1561 ggaggtggcc gatgccgcca ccgtcgacgc ccacgaggag gaggccgacc tcgtcgaccg
     1621 ggccgccgac ctcgaagcgc tcatcgagcg gctcgacgcc gccaccgccg agcacctcga
     1681 tacggcggcg gggatcgaca aggcggtgtc cgacatcggt gccctccgca ccaaggtcga
     1741 cagcctcacc aagcaggtgg cggagaagcg agaggcccgg gcggcgcgcc agcagcggct
     1801 cgacgtgctc ctcggctgcc gcaacacgct cgacgcggcc atcgcccagc tggccggcgg
     1861 ccacgtcgcc gccacggatg cggtgggcgt gctcgaccag ggtgcgaagc ggtgccaggc
     1921 cgcgctcgag atcgcccagg gcgacaccgg cgccgtccac ccctacgact tcccggaccc
     1981 gagcctgctc atcgtcggcg acacctacta cgcctacgcc accgaggggc cgggcggggc
     2041 catccaggtc ctgtcgtcga aggacctgca gaactggtcg gtgcggaccc gggcactggc
     2101 caaccgcccc gagtgggcga aggcggggcc gatctgggct ccgagcgtcc acgccatcgg
     2161 cggccggtac gtcctctact acgccactcg ctcgaagatc tcgagccagc agtgcatcag
     2221 caccgcggtg agcaacgacc cggccgggcc gttcatcgac ctctcaccgg ttccgctcat
     2281 ctgccaagac gacctcggag gctcgatcga ccccgacgtg tacctcgacc agttcggctt
     2341 cgcccacctc acctggaaga gcgaggggga gacggtcggc ggccgggcga agatctggac
     2401 ccaggcgctg agcgccgacg ggttggcggt caacgggttc gccaagtcga tgttcgaggc
     2461 ggaggacggc tgggagcaga agaccgtcga gaacccgtcg atggtgctcc tcgacggcca
     2521 gtgggtcctc ttctacagcg gcggcaagtg gaactccgag ggctactcgg tggggtacgc
     2581 cctctgcgac ggcgccatcg gcccgtgcca ccgcccggtc aacccgcgcc tccacaccag
     2641 tgccggcggc ttcgtcggcc ccggcggcgc cgacgtgttc cgcagccggg gcgggcgctc
     2701 gctcgccgtg tacgccgcct gggacaaggg caaggtcggc aacccgaacg cccgccgcct
     2761 ccacgtggcc gaggtgtcgt tccacgacgg cgcggtccac tggaaggacc tctgacacct
     2821 ggtcagttcg tggtggcggt accggcgtcc gtgtccgggg ccaggtcgtg caggtaggcg
     2881 atgaggtcgg cgacctcggc atcgctcagc tcgccgtcgt agacgggcat gacgttggcg
     2941 tacccgtcga ccacctggcc ccgcgactgc aggatcgcct cgcgcaggta ggtgtcgtcg
     3001 gcgacgatct cggtcccgtc gtcgagggtg accgtggagc cggccaggcc ctgccaggtg
     3061 gggccggtgc ccttgccgcc gtcgacggag tggcagctga tgcagccctg ctccttggcg
     3121 acctgcctgc cgcgctcgcc cgccgcgctc agcttcacct caggaccacc gccgtcgctc
     3181 ccgcaggcgg cgagcagggc gccgaggccc aacgacaccg cgacggtgac ggcgatcagc
     3241 gagcggcgca cgggcgtcag gcttcgggag cggcatccgc ctcggcggcc ggtgcctcgg
     3301 cgtcgtcggc cgcagcgggc tcctcgaccg gggcggcggg ctcggggtcc gcctgggcat
     3361 cggcggcggc gtcctcggca gcgaggtcga cgcccggctc ggcctccgca gcctcggggg
     3421 tgtcgggggt gtcggggtag aagcggtaga ggttctcgat cacctcgagc aggcccgtgg
     3481 agtcgaagcc cttgttgagg tcgaccgtga ccatgttgcc gttgatctgc acgccggcga
     3541 ccgacggctt gtccagcagg cggcgggcca gctcgtcagg gggacgctcg ccccagatct
     3601 cctgaccggc gaggtaccgc tcgtggtcca tgcccgtgaa ggctcggttg gtctcgaagc
     3661 ggatgacccc ggggttcgag gacggcttct cgttgacgac gatcgactgg cccatggcag
     3721 cggcgatcgt agccccgcca cctggggcga cccccaaacc gcccatcccc cacctggcag
     3781 agccgcgtcg gtacggtcgc acccatggag gcccagcgac gcacggtcga cgtgcggtac
     3841 gtggccctca cgaccttcgt cgccgtcagc gtggtcatcg gcgtcgtcgg gtcgatctgg
     3901 gcggtcggcc gcctgtggcg ggtcgtgtcg tacctgctca tcgctctgtt cttcgccgcc
     3961 gtgctcaccc cggcggtgga cttcctccag taccgggcga ggatgcgccg gggcgtggcc
     4021 acgctgctgg tcttcctggc cggcctcgcc gtgctctcgg cgatgatcta cgcgttcgtg
     4081 cggcccatcg tcgaccaggg gcagaagttc tccgacagcc tccccgagat gatcgacgac
     4141 gcccaagagg gcaagggccg gctcggcgag ctgatcacga agtacgacct cgaggagaag
     4201 ttcaacgaga accgcgaggc catcgacaag cagctgaaca acgcgggcag caagggcatc
     4261 accatcctcg gcacggtctt ttcgggcatc gtcgccgccg tcacggtgat ggtgctcacg
     4321 atcctgctgc tgctggccgg tcccgacctg agccagtcgt tcctgaacat catcccggag
     4381 cctcgacggg accgggtgag acgggtcgcg tccgatgcgt cgatggcggt gacgggctac
     4441 atgtacggca acgtcgtgat cagcatcctg gcgggcatcg ccgcctacat cttcttgcgg
     4501 atcgccggca tcccctaccc cgaggtgctg gcgctgtggg tggcgttcgc cgacctgatc
     4561 ccgctcgtcg gtgccaccct cggcgccatc ccgccgatcc tggtggcctt cctcacgtcc
     4621 acgcccgcgg gcatcgccac catcgtcttc ttcgtcctct accagcagtt cgagaacaac
     4681 gtcctgcagg tcacggtcat ggcccgcacg gtgaacatca acccgctcgg ggtgctcgtg
     4741 tcggtcctga tcggcgtcga ggtcttcggc ctgctgggcg cgctgctcgc catcccggtc
     4801 gccggtgtca tccaggtggt cgtccgcgac ctgtgggacg agcgggccgg acgccccaag
     4861 gccgagatca ccctcggcac cgacgagatc ccgatgtccg agatcgaccc gcccgcctcc
     4921 cccgcctgat cctgccggga ccggcgccgg gcgtcacggc tcgacggcgc cgtcctcgca
     4981 cgccgaagga gcggtgaccg ggttgcagcg agcggcgagg aagcccggca gcgccgcggt
     5041 gtagtcggtg ccgtactgcg cctcggcgcc gtcgggaccc gggaagtcgg tgctcaccca
     5101 ctgggccccg ctggcgagcg ccgcgtcgag gcgggtggtg tcaccggtct cggcgtcgac
     5161 cccgttcgca tcggcgcggg tgcgcacgag gtagccctgc tgcaccagct cggtgatgcg
     5221 cttctggtcg gcgaccgggt cgtcgaggcc gacgtacgac gcgtcgggct ggccgggctc
     5281 ggcgttggtg aacaggatcc ggccctcgag cccgtcgtgg ccctcgaggt agatcgagcg
     5341 gta
//
LOCUS       DATMFS010000131         4503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_284380, whole genome shotgun sequence.
ACCESSION   DATMFS010000131 DATMFS010000000
VERSION     DATMFS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284380"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VNQ33_02455"
     CDS             <1..394
                     /locus_tag="VNQ33_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HWJ96990.1"
                     /translation="PTWPDVERPADLVEGDPAAVYADVARRCRAAFEGADLDLEMDTP
                     MGKRTVADRLAFPAVDLYVHAWDLGRSAGIEVAIPGDVVAFAHSYIDPIPDEAVRGPK
                     GAFGPEVAVPDDASATDRFVAWTGRDPA"
     gene            complement(434..922)
                     /locus_tag="VNQ33_02460"
     CDS             complement(434..922)
                     /locus_tag="VNQ33_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96991.1"
                     /translation="MTRSAPAAPDGTITSRCSRTFPLQHAAERAEDPGMERDHIERRI
                     GVRRQIRSVPMAWCRDPANPSPYDAQVRPDGNLVEVSVSGAGLIAVTHPYLQEGTKVL
                     IACLGAVGPVIARRIEPDVYPGESYYGVEFCEPNGRLAQTVYSTFLAKTRDAPDVYLP
                     RD"
     gene            complement(894..1898)
                     /locus_tag="VNQ33_02465"
     CDS             complement(894..1898)
                     /locus_tag="VNQ33_02465"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ96992.1"
                     /translation="MATVQEDTASLDDFEQYRGELIGYCYRMLGSSSEAEDAVQDTFT
                     KAWRNLDRFEGRSSLRTWLYKIASNACFDQLRSAQRRARPMDLSESWRVDGPIGEQLP
                     EVTWIQPIPDRLVRGSTADPAEVAEGRDTVRLAFISALQHLPPKQRAALILCEVLRWP
                     AADVADLLDTTVASVNSALQRARATLDEVNARTVTNAVPAEQQVDPAVLDRWVAAFEA
                     YDIDALTMLVREDAIQSMPPYELWLQGRDQVIRWWVGPGAECAGSRLLPTSANGLPAF
                     GQYRPSGPGGRHEPWALQVLEFDGDQVAEFSFFLDTDTLFPLFGLPRHPDDPVGPGGT
                     "
     gene            complement(1953..3785)
                     /locus_tag="VNQ33_02470"
     CDS             complement(1953..3785)
                     /locus_tag="VNQ33_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWJ96993.1"
                     /translation="MGNGRRSALFGLVAAGIAVGAVACSSGGGSDGAVAKAPGAATYE
                     SNCATCHAPDGSGGQGPAIGAGKAKAKYTEAEMIGIVTDGRNSMPAWKGTLSGEEIDQ
                     VVAYVRDDLGRKAPSASTTAAKGGAERGSLSGTPPEMEAEGDWAIANGDYASTRTVAD
                     PKITSKNVDDLELAWTYEAKGGGTFGNLTTTPIVAGDHVYLGALTTEVHSIDRKTGKG
                     AFVVGDQVGIFGPTGVGIGWGQLYGTKANDEGKGSMVVAYDAETGRENWATDIGEHGG
                     IINMQPSAYDGLVFASTSGYGNGTIATIYALDAGNGSIVWDFPVIADPGLWGNPGLNS
                     GGGVWYPPTIDTRRRVAYFGTGNPYPFPGAEGFPNGSSRPGDNRWTDSTVALDIDSGK
                     LVWGHQAIPHDIFDRDNMLAARTTIEVDGKERDVVISSGKLGIVYALDADTGDEIWKT
                     PVGKHQNDELTEIDGPTLVYPGSLGGVQTPLAIADGTVYACVMNAPTEYAGPEETSYG
                     FTVQLGKANSQMVAIDADTGSIEWEVDLPGDSFGGSTVSGDLVFTSSFGGEVLALDRA
                     TGKTVWSYEAPGGINGWPAIAGGELYVPVGMADPPQLLKFELPD"
     gene            3932..4384
                     /locus_tag="VNQ33_02475"
     CDS             3932..4384
                     /locus_tag="VNQ33_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96994.1"
                     /translation="MPATSPATRQPRARRALAGLAVALTVLGAGACSSPAPSQRELTE
                     ALVDSGLSKKVADCAASALTDSLTKSELAELTERGAGGAPVDDPAKDDDSYDKLQKAM
                     SACREIQAQAAPTTTVAPAAEAGSSTTVTIADGTEGAELNPASTTTTP"
BASE COUNT          592 a         1626 c         1609 g          676 t
ORIGIN      
        1 gcccacctgg cctgacgtcg aacgtcctgc tgatctcgtc gagggcgacc ccgccgccgt
       61 gtacgccgac gtcgcccggc gctgccgcgc cgcgttcgaa ggcgcggacc tggatctgga
      121 gatggacacg ccgatgggca agcgcaccgt cgccgaccgc ctggcgttcc cggccgtcga
      181 cctgtacgtc cacgcatggg acctgggccg atcagccggc atcgaggtcg ccatccccgg
      241 cgacgtcgtc gccttcgccc actcgtacat cgacccgatc cccgacgagg ccgtgcgcgg
      301 cccgaaggga gcgttcgggc ccgaggtcgc ggtccctgat gacgcctccg ccacggaccg
      361 gttcgtggcc tggaccggtc gggatcccgc ctgatccggc ccgcctccgg ccaccggcgc
      421 gggaggaccg ccgtcagtcc cgggggaggt agacgtcggg ggcgtcgcgg gtcttggcga
      481 ggaaggtgct gtagacggtc tgcgccagtc ggccgttggg ctcgcagaac tcgacgccgt
      541 agtagctctc gcccgggtac acgtcgggct cgatgcgccg ggcgatgacg ggtcccaccg
      601 cccccaagca ggcgatgagg accttggtcc cctcctggag gtacgggtgg gtgacggcga
      661 tcagcccggc tccgctgacc gagacctcga ccaggttgcc gtccgggcgg acctgcgcgt
      721 cgtagggcga cgggttcgct gggtcgcggc accacgccat cggcaccgag cggatctggc
      781 ggcggacccc gatccgccgc tcgatgtggt ctcgctccat ccccgggtct tcggcgcgct
      841 cggcggcgtg ctggagcgga aacgtgcggg agcagcgaga agtgatggtg ccgtcaggtg
      901 ccgccggggc cgaccgggtc atcggggtgt cgggggagtc cgaagagcgg gaacagcgtg
      961 tcggtgtcca agaagaagct gaactcggcc acctggtcgc cgtcgaactc gagcacctgc
     1021 agggcccagg gctcgtggcg tccgccgggg ccgctcggcc ggtactggcc gaaggccggg
     1081 aggccgttgg cggaggtggg cagcaggcgg gatccggcgc actcggcacc gggaccgacc
     1141 caccagcgta tgacctggtc ccgtccttgg agccagagct cgtagggcgg catcgactgg
     1201 atggcgtcct cgcgcaccag catcgtcagg gcatcgatgt cgtaggcctc gaacgccgcg
     1261 acccaccggt ccagcaccgc cgggtcgacc tgctgctcgg ccggcaccgc gttggtgacg
     1321 gtgcgggcgt tcacctcgtc gagcgtggcc cgggcccgct gcagcgcgct gttgaccgag
     1381 gcgacggtgg tgtccagcag atcggccacg tcggccgccg gccagcggag gacctcgcac
     1441 aagatgagcg cggcccgctg cttgggcggg aggtgctgga gcgccgagat gaacgccagc
     1501 cgcacggtgt cgcgcccctc ggcgacctcg gccggatcgg cggtggagcc ccggaccaag
     1561 cggtcgggga tcggctggat ccacgtgacc tccggaagct gctcgccgat gggtccgtcg
     1621 acgcgccacg actccgacag gtccatgggc cgggcccggc gctgggctga gcgaagctgg
     1681 tcgaagcagg cgttgctggc gatcttgtac agccacgtgc gcagcgacga gcgcccctcg
     1741 aaccggtcga ggttccgcca cgccttggtg aacgtgtcct gcacggcgtc ctcggcctcc
     1801 gacgacgagc cgagcatccg gtagcagtac ccgatgagct cgccgcggta ctgctcgaag
     1861 tcgtcgagcg aggcggtgtc ttcttgcacg gtggccacgg cggacaccct acgaccgggg
     1921 agtgacagcg aggccggggc ctcggtgccg catcagtccg gcagctcgaa cttgagcagc
     1981 tgcggtgggt cggccatccc gaccggcacg tagagctcgc cgccggcgat cgccggccag
     2041 ccgttgatgc cgccgggcgc ctcgtagctc cacacggtct tcccggtggc ccggtcgagc
     2101 gccaggacct cgccgccgaa cgacgacgtg aacacgaggt cacccgacac ggtcgacccg
     2161 ccgaacgagt cgcccgggag gtccacctcc cactcgatgc tcccggtgtc ggcgtcgatc
     2221 gccaccatct ggctgttcgc cttgccgagc tggacggtga agccgtagct cgtctcctcg
     2281 ggtccggcgt actcggtggg ggcgttcatc acgcaggcgt agacggtgcc gtcggcgatg
     2341 gcgagcgggg tctgcacgcc gccgagcgag cccgggtaca cgagcgtcgg cccgtcgatc
     2401 tcggtcagct cgtcgttctg gtgcttcccc accggcgtct tccagatctc gtcgccggtg
     2461 tcggcatcga gggcgtagac gatgccgagc ttgcccgagc tgatgacgac gtctcgctcc
     2521 ttgccgtcga cctcgatggt cgtccgggcg gcgagcatgt tgtcccggtc gaagatgtcg
     2581 tgggggatgg cctggtggcc ccacacgagc ttcccggagt cgatgtcgag ggcgacggtg
     2641 gagtcggtcc agcggttgtc gccgggccgg ctcgagccgt tcgggaaccc ctcggcgccg
     2701 gggaacgggt aggggttgcc ggtgccgaag taggcgacgc ggcgcctcgt gtcgatggtc
     2761 ggcgggtacc agacgccgcc gccgctgttg aggccggggt tgccccagag gccggggtcg
     2821 gcgatgaccg ggaagtccca gacgatcgac ccgttcccgg cgtcgagggc gtagatcgtg
     2881 gcgatggtcc cgttcccgta gccggaggtg gaggcgaaca cgagcccgtc gtacgccgaa
     2941 ggctgcatgt tgatgatgcc gccgtgctca ccgatgtcgg tggcccagtt ctccctgccg
     3001 gtctcggcgt cgtacgcgac caccatcgag cccttgccct cgtcgttcgc cttggtgccg
     3061 tacagctggc cccagccgat gccgaccccg gtggggccga agatcccgac ttggtcgccc
     3121 acgacgaacg cacccttgcc ggtcttccgg tcgatgctgt gcacctcggt ggtcagggcc
     3181 cccaggtaca cgtggtcgcc ggccacgatc ggcgtggtgg tgaggttgcc gaaggtgccg
     3241 ccgcccttcg cctcgtaggt ccaggccagc tccaggtcgt cgacgttctt cgacgtgatc
     3301 ttcggatcgg ccacggtgcg cgtcgacgcg tagtcgccgt tggcgatggc ccagtcgccc
     3361 tcggcctcca tctcgggcgg ggtgcccgac agcgagcctc gctcggcgcc gcccttcgcc
     3421 gccgtggtgg acgcggaggg tgccttgcgg ccgaggtcgt cgcggacgta cgccacgacc
     3481 tggtcgatct cctcgcccga cagcgtcccc ttccacgccg gcatcgagtt ccggccgtcg
     3541 gtgacgatgc cgatcatctc ggcctcggtg tacttggcct tcgccttgcc ggcgccgatg
     3601 gccgggccct ggccgccgct gccgtcagga gcgtggcagg tggcgcagtt cgactcgtag
     3661 gtcgctgcgc ccggcgcctt cgccacggcc ccatcagagc ccccgcccga cgaacacgcc
     3721 accgcaccga cggcgatgcc cgccgcgacc agcccgaaca gcgccgagcg cctcccgttc
     3781 cccatgctgg cggaccctac tgcggaggtc cggacctgcc gcgtggagcg tgcgaacctg
     3841 cccgaggccc ccgccctcgc cctgtcgttc gtggccgacg gctgatcgcc tgctccggtc
     3901 gtcgggtcct cggccggtag cctcggcccc cgtgcctgcg acctcgcccg ccacccgcca
     3961 gccccgtgcg cgccgcgccc tcgccgggct cgccgtcgcc ctcaccgtgc tcggtgccgg
     4021 ggcctgctcc tcgccggccc cgtcgcagcg cgagctgacc gaggccctcg tggactccgg
     4081 cttgtccaag aaggttgccg actgcgcggc gtcggcgctc accgactcgc tcacgaagag
     4141 cgagctcgcc gagctcaccg agcgcggtgc cggcggcgcc ccggtcgacg acccggcgaa
     4201 ggacgacgac agctacgaca agctgcagaa ggccatgtcg gcgtgccgcg agatccaggc
     4261 ccaggcggcg cccaccacca cggtcgcccc ggccgccgag gccggctcgt ccaccacggt
     4321 gaccatcgcc gacggcaccg aaggcgccga gctcaacccg gcctcgacca ccacgacccc
     4381 ctgaccgacc gtaggcccag cccaccgctg taccgcccgc caccgaaggc cacggcctcc
     4441 ggtccggcgt tcgatcaggt gagatggggc gctcgggcct agatgtgatg agcggggacg
     4501 ttg
//
LOCUS       DATMFS010000132         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28833, whole genome shotgun sequence.
ACCESSION   DATMFS010000132 DATMFS010000000
VERSION     DATMFS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28833"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VNQ33_02480"
     CDS             complement(<1..683)
                     /locus_tag="VNQ33_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ96995.1"
                     /translation="MHVEILGDDQAVTVVTLDRPERRNALDHETLGELTTALADAQAR
                     PTRVLILTGSSGHFCAGADLQGVEDASFASLLQGVLDRLVNVSFPTMAAIEGAALGAG
                     TQLAVACDLRVATPGASFGIPAAKLGLMVNHWTVQRVATLLGPSTARAVLLAAEVVDG
                     AQALQLGLVNRPGALPEALAWAERIAALAPLTIAGHKLMLNALEPALPTDPAVVAAFT
                     RAWESEDFA"
     gene            complement(722..1720)
                     /locus_tag="VNQ33_02485"
     CDS             complement(722..1720)
                     /locus_tag="VNQ33_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96996.1"
                     /translation="MTDDLTPGPFEAFDADVGERMHAAADHLDGAGVTRSGVAAAVDR
                     RRRQRVRSRALAAGAAAAVAVVGGMAIVTRTDGHGPDRVISAASTTVPGPCAGGSIDD
                     PVGWFRLTAGQAARLEEAGAITADQAADREQHAVLLTYRELQLLDDDPDLDEQLFQLG
                     YEDAAGIGYLRAEGLLTPRQEASVDDGLAPVLVASQVDRLMQHFPDLAAVAAGGDPFS
                     TSAPEVPSGPGRSAFGDGWIDVLRSKGLLTPAQEAAAAAGDGFTLSPAQRQALQEQGD
                     QQAAGTTTTALSFAPGSSPATTEPDPCDGQLPAAPGRGPQARATTSSASAQTTTTT"
     gene            complement(1717..2214)
                     /locus_tag="VNQ33_02490"
     CDS             complement(1717..2214)
                     /locus_tag="VNQ33_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ96997.1"
                     /translation="MTGEVRPRLRVAPETADAAAAFDALFVRERTAMVRVAYLLVGSE
                     PIAEEIVQEAFAAVYARWDRIDNPGGFLRQCVVNGARSSLRRRSLERRKAQLLVDEGT
                     LPAGRELLDALAALPIGWRSVVVLRFYEGMTQEEIAVALDMRLGTVKSSLHRGLAKLR
                     EALEP"
     gene            complement(2281..>2496)
                     /locus_tag="VNQ33_02495"
     CDS             complement(2281..>2496)
                     /locus_tag="VNQ33_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ96998.1"
                     /translation="RRADETADAYARALSLRYREPRLADVGRLIDEATYAPRPPSEPR
                     QQEADALLAQLAAAPVPDEAPAPLGAG"
BASE COUNT          318 a          871 c          993 g          314 t
ORIGIN      
        1 tcggcgaagt cctcgctctc ccaggcccgg gtgaacgcgg cgacgacagc gggatcggtg
       61 ggcaaggcgg gctcgagcgc gttgagcatg agcttgtggc cggcgatggt gagcggcgcc
      121 agcgcggcga tgcgctcggc ccaggccagg gcctcgggga gggcaccggg ccggttgacc
      181 agcccgagct gcagggcctg ggcaccgtcg acgacctcgg ctgccagcag cacggcccga
      241 gcggtgctcg gcccgagcag ggtggcgacg cgctgcacgg tccagtggtt gaccatcaag
      301 ccgagcttgg cggcggggat gccgaacgag gcgccgggcg tggcgacccg caggtcgcac
      361 gccacggcga gctgcgtccc ggcgccgagc gccgcgccct cgatggcggc catggtcggg
      421 aacgagacgt tcaccaggcg gtcgagcacg ccctggagca gcgaggcgaa gctggcgtcc
      481 tcgacgccct ggaggtcggc gccggcgcag aagtgcccgg acgacccggt caggatgagg
      541 acccgggtgg gccgggcctg ggcgtcggcc aaggcggtgg tgagctcccc cagggtctcg
      601 tggtccaggg cattgcggcg ctcgggccga tcgagggtga ccacggtgac ggcctggtca
      661 tccccgagga tctcgacgtg cacggtcaac ggtcaacctc cggtcagcgg accggctgac
      721 cctaggtggt cgtggtggtc tgggcggacg ccgaggaggt ggtggcgcgc gcctgcggac
      781 cgcggccagg cgcggccggg agctgaccgt cgcacggatc gggttcggtg gtggcgggtg
      841 acgagcccgg ggcgaacgac agcgccgtcg tggtggtgcc ggctgcctgc tggtccccct
      901 gctcctggag cgcctggcgc tgggcgggcg acagggtgaa gccgtcgccg gccgccgccg
      961 cggcctcctg cgccggcgtg agcaggccct tcgaacggag gacgtcgatc cacccgtcgc
     1021 cgaaggcgct cctcccggga ccggacggga cctcgggggc cgaggtgctg aacgggtcgc
     1081 cgccggccgc caccgcggcc aggtcgggga agtgctgcat cagccggtcg acctgggacg
     1141 ccacgagcac gggggcgagg ccgtcgtcga cgctcgcctc ctggcgcggg gtcaacaggc
     1201 cttcggctct gaggtagccg atgccggcgg cgtcttcgta gccgagctgg aacagctgct
     1261 cgtcgaggtc cgggtcgtcg tcgagcagct gcagctcgcg gtacgtgagc aagacggcgt
     1321 gctgctcccg gtcggcggcc tggtcggcgg tgatggctcc tgcttcctcc aaccgggcgg
     1381 cttggcccgc ggtcagccgg aaccacccca cgggatcgtc gatggagccg ccggcacagg
     1441 ggccggggac ggtggtggat gccgccgaga tcacgcggtc ggggccgtgg ccgtcggtgc
     1501 gggtgacgat ggccatgccg ccgaccacgg cgacggccgc ggcggccccg gcagcgaggg
     1561 cacgggagcg gacccgctgg cgtcggcgtc gatccaccgc ggcggccacg ccggagcggg
     1621 tcaccccggc gccgtcgagg tgatccgccg ccgcgtgcat gcgctcgccc acgtcggcgt
     1681 cgaacgcctc gaaggggccg ggggtcaggt cgtcggtcac ggctccagcg cctcccgcag
     1741 cttggccagt ccccggtgca ggctggactt caccgtgccc agccgcatgt cgagggcgac
     1801 ggcgatctcc tcttgcgtca tgccctcgta gaaccggagg accacgacgg accgccagcc
     1861 gatcggcagg gcggcgagcg cgtcgagcag ttcccgcccc gcggggaggg tgccctcgtc
     1921 gacgagcagc tgggccttgc ggcgctccag cgagcggcgg cgcagggacg accgcgcccc
     1981 gttcaccacg cactggcgga ggaacccgcc cgggttgtcg atccggtccc agcgggcgta
     2041 caccgcggcg aacgcctcct ggacgatctc ctcggcgatc ggctccgaac ccaccagcag
     2101 gtacgcgacc cggaccatcg cggtgcgctc tcggacgaac agcgcgtcga acgccgcggc
     2161 ggcgtccgcc gtctccgggg caacccgcaa ccggggccgg acctctccgg tcatcgcggc
     2221 ctccgccgtc gtcgtgtgca cgtccctacg aggcacaggg tccgctgatc gttcccgcgc
     2281 tcaaccagcg cccaggggcg ccggcgcctc gtcggggacg ggagcagccg cgagctgggc
     2341 gaggagggca tcggcctcct gctggcgcgg ctcggagggt ggacggggcg cgtacgtggc
     2401 ctcgtcgatg agccgtccga cgtcggcgag ccggggttcc cggtagcgca gggagagggc
     2461 gcgggcgtac gcgtcagccg tctcgtcggc tcggcg
//
LOCUS       DATMFS010000133         3475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28906, whole genome shotgun sequence.
ACCESSION   DATMFS010000133 DATMFS010000000
VERSION     DATMFS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28906"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1667
                     /gene="guaA"
                     /locus_tag="VNQ33_02500"
     CDS             93..1667
                     /gene="guaA"
                     /locus_tag="VNQ33_02500"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWJ96999.1"
                     /translation="MTNPDPVVAASSETGPILVVDFGAQYAQLIARRVREAHVYSEIV
                     PHTITAAEVAARNPAGIIFSGGPKSVHVEGAPVIDPAVYDLGTPVLGICYGAQLIAQQ
                     LPGGVVANTGVGEYGRTELTVTAPDAVVFGGGQPVTQQVWMSHFDSISVPPDGFSITA
                     STPAAPVAAMEDVARKIHAVQFHPEVVHTPHGQAVLEHWLYDVCGCPPSWVMSSVIDT
                     QVELIRKQVGSARVICALSGGVDSAVAAALVHKAIGPQLTCVFVDTGLMRLGEGEQVV
                     ETFQRHQGIELIHVRAADRYFERLAGVIDPEDKRKAIGELFIRIFEDVSGGIEDAKFL
                     VQGTLYPDVIESGTASAAKIKSHHNVGGLPEDMQFDLVEPLRALFKDEVRAVGSELGL
                     PDEIVWRQPFPGPGLGVRIIGEVTPDKVAILQRADAIVREEIRSAGLEREIWQAFAVL
                     PDIRSVGVMGDERTYGYPAIIRAVTSEDAMTADWARLPYDLLERMSSRIINEVDGINR
                     VAYDVTSKPPGTIEWE"
     gene            2194..>3475
                     /locus_tag="VNQ33_02505"
     CDS             2194..>3475
                     /locus_tag="VNQ33_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ97000.1"
                     /translation="MDPDTTAEGGQVGGSGNEVATLADRHADDVAALHRVASLPPGDP
                     DERLTQLLSAGCRALGVGRGFVLLRGPAGLIVRVVAGPDAVGPEAGETVSDRRLDDVL
                     ARQATVAALGGPGATDDRAPLGGGSMVACPLWVTGRVAGAVAFADSTAREPFSSWQLA
                     LVDLVADGASRILEHEADIRAMVRLESQAQAMIDLMPDPVVRLRRDGQQLTDGGDHVL
                     GVFDPTASARPAGTPPDPATLELVQRAVAAALDTGRLQTDVYAAGPGLDARRVEARFV
                     PGDDDVVLCIVRDITERHRAELALAEQVAFEALVASISTRLIGCAPLLLDEAIDVGLG
                     EIASFFGADTAFIDELAPDGSTLRVSHQWSRRRTVPTRTRGQKVDVKGFGWLTARFER
                     AGHVFVRGPGTIPREATEQSLVDADDKGVLWVRLG"
BASE COUNT          493 a         1288 c         1177 g          517 t
ORIGIN      
        1 gccggcgggc tgcgccgctc catggcgtcg tgcggctacc ggaccctgaa ggagctccag
       61 aaggccgagc tcacgatcac cccggtgcac ccatgaccaa ccccgatccc gtcgtcgccg
      121 cctcctccga gaccgggccg atcctcgtcg tcgacttcgg cgcccagtac gcgcagctca
      181 tcgcccggcg cgtccgcgag gcgcacgtgt acagcgagat cgtcccgcac acgatcaccg
      241 cggcggaggt cgccgcccgc aacccagccg ggatcatctt ctccggcggg ccgaagtcgg
      301 tccacgtgga gggcgcaccg gtcatcgacc ccgcggtgta cgacctggga accccggtcc
      361 tgggcatctg ctacggcgcc cagctcatcg cccagcagct gcctggcggt gtcgtcgcca
      421 acaccggtgt cggcgagtac gggcgcaccg agctcacggt caccgcaccc gatgcggtgg
      481 tgttcggtgg cggtcagccc gtcacccagc aggtgtggat gagccacttc gactcgatct
      541 cggtcccgcc ggacgggttc tcgatcacgg cgtccacacc ggcggctccg gtcgccgcga
      601 tggaggacgt ggctcgcaag atccacgccg tgcagttcca cccggaggtc gtgcacacgc
      661 cgcacggcca ggcggtcctc gagcactggc tctacgacgt ctgcggctgc cccccctcgt
      721 gggtcatgag ctcggtcatc gacacccagg tcgagctgat ccgcaagcag gtcggttcgg
      781 ctcgggtcat ctgcgccctg tcgggcgggg tcgactccgc ggtcgcggcg gcgctggtgc
      841 acaaggcgat cggaccccag ctcacgtgcg tgttcgtcga cacgggcctc atgcgcctcg
      901 gcgagggcga gcaggtggtg gagacgttcc agcgccacca gggcatcgag ctgatccacg
      961 tccgggccgc cgaccgctac ttcgagcggc tggctggcgt catcgatccc gaggacaagc
     1021 gcaaggccat cggcgagctg ttcatccgca tcttcgagga cgtctccggc ggcatcgaag
     1081 acgccaagtt cctggtccag ggcaccctct atcccgacgt catcgagtcg ggtaccgcca
     1141 gcgccgccaa gatcaagagc caccacaacg tcggtggcct gcccgaggac atgcagttcg
     1201 acctggtcga accgctccgg gcgctgttca aagacgaggt gcgggcggtc ggcagcgagc
     1261 tgggcctgcc cgacgagatc gtctggcgcc agccgttccc cgggcccggc ctcggcgtcc
     1321 gcatcatcgg cgaggtcacg ccggacaagg tcgccatcct ccagcgcgcc gacgccatcg
     1381 tgcgcgagga gatccgctcc gccggcctcg aacgcgagat ctggcaggcc ttcgccgtgc
     1441 tgcccgacat ccgttccgtg ggcgtcatgg gcgatgagcg cacctacggg tacccggcca
     1501 tcatccgcgc cgtcacctcc gaagacgcca tgaccgccga ctgggcccgc ctcccgtacg
     1561 acctcctcga gcgcatgtcg tcgcgcatca tcaacgaggt cgacggcatc aaccgagtgg
     1621 cctacgacgt gacgagcaaa cccccgggca ccatcgagtg ggagtgaccc tcgtcgcggg
     1681 ccgagaaggg tgaaggggcg cgccggtatc ccgaccgagt ggtcggtttg gagcctggtg
     1741 ctggtctgag gacccgcaca cgtcaccttc cggaggcgtc gggaccggcc cgggtgggat
     1801 cgatgggtgt gccgctcgtc ggccctgcag gatcggagcc agcttctgag ggccgctcac
     1861 ccgtcgccat gggtggcggc tccgcctcga cgtgtcgact cctcctgtga aacgaacgta
     1921 tgttcgacct ctgtcacggt tctttccgat caccctcgac ccctgcgatc gcccccacgg
     1981 atgccggaca tgccgggtgg cgggtctgat gtcgtctccg atccgacggc ccggtccttc
     2041 accgctgggg cggcgcgggc gtggcgtgct cgaccgagag cgtcacgaca tgtggcccga
     2101 tccctggaaa tctccagtcg gcgtgccgat ggggaaaaca cccagaaggg tgacgacgac
     2161 acggcacggc gccgttacgg tgcaaaggaa cgcatggatc cggacacgac ggccgagggt
     2221 gggcaggtcg gtggctcggg caacgaggtg gcgaccctcg ctgaccgtca cgccgatgac
     2281 gtcgccgcgc tccatcgcgt cgccagcctc cctccgggcg atcccgacga gcggttgacg
     2341 cagctgctca gcgccgggtg ccgggcactc ggcgtgggcc gggggttcgt gttgctccgc
     2401 ggaccggccg gactcatcgt ccgcgtcgtc gccggacccg acgccgtcgg ccccgaagcc
     2461 ggcgagaccg tgtcggatcg gcgcctcgac gacgtcctcg cccgccaggc cacggtcgcc
     2521 gcgctcggcg gacccggcgc caccgacgac cgcgctccgc tcgggggcgg ttcgatggtg
     2581 gcctgcccgc tctgggtgac gggccgcgtc gccggcgccg tcgccttcgc cgactcgacc
     2641 gctcgcgagc cgttctcgtc gtggcagctc gccctcgtcg acctcgtggc cgatggcgcc
     2701 tcgcgcatcc tcgagcacga ggccgacatc cgggccatgg tccgcctcga gtcccaggcc
     2761 caggcgatga tcgatctgat gcccgatccc gtcgtccggc tccggcgaga cggccagcag
     2821 ctcaccgacg gcggcgacca cgtcctcggc gtgttcgacc cgaccgccag cgcccggcca
     2881 gccggcacac caccagatcc ggccacgctc gagctggtgc agcgggccgt cgccgccgcc
     2941 ctcgacaccg gccggctcca gaccgacgtg tacgccgccg gccccggcct cgacgcccgg
     3001 cgagtcgaag cccggttcgt gcccggcgac gacgacgtcg tgctgtgcat cgtccgcgac
     3061 atcaccgaac gccaccgggc tgagctggcc ctcgccgagc aggtcgcgtt cgaagccctc
     3121 gtcgcatcga tctccacccg gctcatcggg tgcgctccgc tgctgctcga cgaagccatc
     3181 gacgtggggc tcggcgagat cgcctcgttc ttcggggccg acacggcctt catcgacgag
     3241 ctggctcccg acggctcgac cctgcgggtg tcgcaccagt ggtcgcggcg acggacggtc
     3301 ccgacgagga cccggggaca gaaggtcgac gtcaagggct tcggctggct gaccgcccgg
     3361 ttcgagcgcg ccggccacgt cttcgtgcgc ggccccggga cgatcccccg ggaggccacc
     3421 gagcagtccc tcgtcgacgc cgacgacaag ggcgtcctgt gggttcggct cggct
//
LOCUS       DATMFS010000134         2659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_28916, whole genome shotgun sequence.
ACCESSION   DATMFS010000134 DATMFS010000000
VERSION     DATMFS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28916"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VNQ33_02510"
     CDS             complement(<1..1816)
                     /locus_tag="VNQ33_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ97001.1"
                     /translation="MTSDRSPGEWNLAVRQQEAVARIGQLGLRSGDLDDLLREAMALA
                     AETLEAHSLLLFEVRPDGRHVRGRAGMHGGEVVSRHRIDRIEVPVGRGSLPGYALDQR
                     APVVAEDLLADPRFEALGPQPGIDGRAALAAPISWGEVPWGVLVVSSAEVRAWSDDEV
                     HFVQSVANTMGLAIQRSAVERELRESTTRLDLSLSAGGLGAWSWDLEGDRLLLNSSAL
                     AMYGLVPGRFTGRGVDFMAVIHPEDRARFPQGPEDGPSPSIGHNVFRIIRPDTGELRW
                     IEIWGRPLQEGSPELHLVGVCSDVTERRLAEERQAEILASEHEARVAAEEARERLAFL
                     AEASAVLSTSLDPEVTLLSLGELCIPGLADICFIDLIGDDGFLHDEVGLGSTEVKLHD
                     AQMLRKRRMELGEAAPTSTGHLEALQGRGLLYQSIDDGQLARAAADPDHLALFRRFDA
                     RSTIMAPLVARGRVIGVITLVRCGDQKLFDDDDLAMIEELAARAALATDNGRLFRSRN
                     RVARSLQEALLPPAMPEIEGLALAARYDVAEADVAIGGDFYDVMPVGPSAWGVVVGDV
                     CGRGPDAAALTGLVRHTLRTAVVREHLPSRVLGQANLAM"
     gene            complement(1874..>2659)
                     /locus_tag="VNQ33_02515"
     CDS             complement(1874..>2659)
                     /locus_tag="VNQ33_02515"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWJ97002.1"
                     /translation="RAALALDNSRLFHSRNRVARSLQAALLPPALPSIPGVGLAARYD
                     VSAAEADVIIGGDFYDVIPVAPGSWGMVVGDVCGRGPSAAALTGLVRHSLRTAVVREL
                     VPSRVLAQTNQAMLRQIDDASFCTAAYVRIDVDDPLSGHVVVSASSAGHPRPILVRAD
                     GHAEALDCAGTLLGVVADPQLVDISVDLGPGDAVVLYTDGVTEARRGGELFGEARLVE
                     VLASLAGGSAEEIAGGLDEQVVDFRRSARDDTAILVIEALAPA"
BASE COUNT          385 a          954 c          997 g          323 t
ORIGIN      
        1 gcatcgccag gttggcctgg ccgaggaccc ggctgggcag gtgctcgcgg accacggcgg
       61 tgcgcagcgt gtggcggacg aggccggtga gggcggcggc atcgggcccg cgtccgcaga
      121 cgtcgccgac cacgacgccc caggccgagg ggccgacggg catcacgtcg tagaagtcac
      181 cgccgatggc gacgtcggcc tcggccacgt cgtagcgggc ggcgagcgcc aggccctcga
      241 tctcgggcat cgcgggcggg agcagcgcct cctgcaacga ccgggccacg cggttgcggg
      301 agcggaacag gcggccgttg tcggtggcga gcgcggcgcg cgccgccagc tcctcgatca
      361 tcgccaggtc gtcgtcgtcg aacagcttct ggtcgccgca gcgcaccagc gtgatgacgc
      421 cgatcacccg gcctcgggcg acgagcgggg ccatgatcgt ggaacgggcg tcgaagcgcc
      481 ggaacaacgc caggtggtcg ggatccgcgg cggcgcgggc aagctggccg tcgtcgatgg
      541 actggtacag caggcccctg ccctggagcg cttcgaggtg cccggtggac gtgggagcgg
      601 cctcgcccag ctccatgcgg cgcttgcgga gcatctgggc atcgtggagc ttcacctcgg
      661 tggaccccag gccgacctcg tcgtggagga acccgtcgtc gccgatcagg tcgatgaagc
      721 agatgtcggc aaggcccggg atgcagagct cgccgaggct gaggagcgtg acctccgggt
      781 ccaggctggt gctgagcacg gcgctggcct cggccaggaa cgccagccgc tcgcgcgcct
      841 cctcggcggc gacccgcgcc tcgtgctcac tcgccaggat ctcggcctgg cgctcctcgg
      901 cgaggcgccg ctcggtgacg tcggagcaga cgccgacgag gtggagctcc ggcgatcctt
      961 cctggagcgg gcggccccag atctcgatcc agcgcagctc accggtgtcg gggcggatga
     1021 tccgaaagac gttgtgcccg atcgacgggc tcggcccgtc ctccgggccc tgcgggaacc
     1081 gagcccggtc ttcggggtgg atcacggcca tgaagtcgac cccccgaccg gtgaagcggc
     1141 cggggacgag cccgtacatc gccagcgccg acgagttgag caggagccgg tcgccctcca
     1201 ggtcccacga ccacgcaccg aggccgccgg cgctcagcga caggtcgagc cgggtcgtgc
     1261 tctcgcggag ctcgcgctcg accgccgagc gctggatcgc cagccccatg gtgttggcga
     1321 cggactggac gaagtggacc tcgtcgtcgc tccaggcccg cacctcggcg ctggagacga
     1381 cgagcacccc ccagggcacc tcgccccagc tgatcggcgc ggcgagcgcg gcgcgcccgt
     1441 cgatgcccgg ctggggcccc agcgcctcga accgaggatc ggcgagcaga tcctcggcca
     1501 cgaccggggc ccgctggtcc agggcgtagc cgggaagcga tccccggccg accggcacct
     1561 cgatgcggtc gatgcggtgc cggctcacca cctcgccccc gtgcatgccg gcccgacccc
     1621 ggacgtggcg gccgtcgggc cgcacctcga acaggagcaa ggagtgcgcc tcgagggtct
     1681 cggcggccag ggccatggcc tcgcgcagca ggtcatcgag gtcgcccgaa cggaggccga
     1741 gctgaccgat ccgggccacc gcctcttgct gtcggaccgc gaggttccat tcgccggggc
     1801 tgcggtccga ggtcatccag cgcaaggcta ccgacccccg tgccgtcgga ccgggccgaa
     1861 ggagaccggg tgatcaggcc ggtgcgaggg cctcgatgac caagatcgcc gtgtcgtcgc
     1921 gggcactgcg ccggaagtcg accacctgct cgtcgaggcc accggcgatc tcctcggcgg
     1981 acccgccggc cagcgatgcg agcacctcca ccagccgcgc ctcgccgaag agctcgccac
     2041 cccgccgggc ctcggtgacg ccatcggtgt agagcaccac ggcgtcaccc gggcccaggt
     2101 cgacggagat gtcgacgagc tgcgggtccg cgaccacccc gaggagggtc ccggcgcagt
     2161 cgagcgcctc ggcatggccg tcggctcgga cgaggatggg ccgggggtgt cccgcgctcg
     2221 atgccgacac gacgacgtgc cccgacagcg ggtcgtcgac gtcgatgcgc acgtaggcgg
     2281 ccgtgcagaa gctggcgtcg tcgatctgcc ggagcatcgc ctggttggtc tgggcgagga
     2341 cccggctcgg caccagctcg cgcacgacgg cggtgcgcag gctgtggcgg acgagtccgg
     2401 tgagggcagc ggcagacggg ccgcgcccgc acacgtcgcc cacgaccatc ccccacgacc
     2461 cgggggccac cgggatgacg tcgtagaagt caccgccgat gatgacgtcg gcctcggccg
     2521 ccgacacgtc gtaccgggcc gccaggccga cacccgggat cgacggcagc gccgggggca
     2581 acaaggcggc ctgcagcgag cgggcgactc ggttgcgcga gtggaagagc cggctgttgt
     2641 cgagcgccag ggcggctcg
//
LOCUS       DATMFS010000135         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_191759, whole genome shotgun sequence.
ACCESSION   DATMFS010000135 DATMFS010000000
VERSION     DATMFS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191759"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNQ33_02520"
     CDS             complement(<1..278)
                     /locus_tag="VNQ33_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWJ97003.1"
                     /translation="MPKKPPKPKLPGLLKGMGVTFNEMTQTIWPGEGAKRFMQPPSPA
                     KGAATVQYPHEKETPPTRARGVIALKEDNCTVCMLCARSCPDWCIYIEG"
     gene            complement(278..1324)
                     /locus_tag="VNQ33_02525"
     CDS             complement(278..1324)
                     /locus_tag="VNQ33_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWJ97004.1"
                     /translation="MSAPIFGIELSYWQETVLRSVGILAAVLLPAGTFVYLFLFKMVS
                     FMQSRLGPMEAGPFGSMQLLAEVGKFLQKEDIVPERADRKLFKLAPYLVVATVLLVYL
                     AVPFGPQMTFVTNFDGGIFYVLAVSSISALGVLLAGWASANKYSLIGGLRAAGQLIAY
                     ELPMVLAVIGVVIQAGTLNMSGIVSAQANGAIFGFDGLGNPYFFTQIIGFCIFMIAVQ
                     AELGQTPFDMPIAESELVTGYITEYSGLRFLLFFIGEFASAGAFAAVAATLFLGGWAV
                     PAAWADLDSAIFNLLGPIIMLTKMMILGGLIFFVRFTFPRFREDQLQKLAWTVLIPIS
                     LANILATTILKVAF"
     gene            complement(1321..2496)
                     /locus_tag="VNQ33_02530"
     CDS             complement(1321..2496)
                     /locus_tag="VNQ33_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HWJ97005.1"
                     /translation="MTALTERQQLAYANAQAADGRVNLEFETDEGMTLNMGPQHPATH
                     GTLRIVVKLDGEEVISCEPVMGYMHRGYEKLTEVRTYPQVTTLINRIDWLGSFANEVP
                     FILAAEKLMDIEAPPRAQWIRTVLFELSRIANISLFVGDMALHIGAISAAFYAFRDRE
                     YVLNQIEAATGGRFHPNFDRIGGLKDDLPKGWIADTKASMEKIRDFCDEMETLVEGNE
                     IFQARTRGIGVIPADVALAYGMTGANLRASGVDWDLRRDQPVGLAWNECDWNVVTHPD
                     GDSFARFWVRLQEIRESTKIVDQLLDGLPAGPIMAKVPRIIKVPEGEAWVATENPLGE
                     MGYYVVSKGDLGPFRVKIRTASFNNVAVIPWVTRGVYVPDVVTILASLYFILGDIDR"
     gene            complement(2493..3152)
                     /locus_tag="VNQ33_02535"
     CDS             complement(2493..3152)
                     /locus_tag="VNQ33_02535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWJ97006.1"
                     /translation="MEETPEAPEVDEARQAVIDKLTEELGEAVVGSHIRPGDDVWVRV
                     TAESWRTAGLVVRDHLSFRAFEYLSAIDWLPNPYGKNEDAPTDPRPEPNTDIVTGYAG
                     GDTRFQVIARVQSYTSKLGLNLKVDLPDDDLSLATWLPVYAGVNWHEREVHEMFGITF
                     TGHPYLQNLYLPGEFEGHPLRKDFPLLARFVKPWPGIVDVEPMPASDDEAEAETEEAA
                     E"
     gene            complement(3157..>3612)
                     /locus_tag="VNQ33_02540"
     CDS             complement(3157..>3612)
                     /locus_tag="VNQ33_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWJ97007.1"
                     /translation="LLNTSRKYSLWIFQWGLACCAIEMGAALGAPRYDVMRLGVIPLP
                     ASPRQADLVCISGTVTDKMVPAIRRLYDQMPDPKYVISMGSCANCGGPYWDSYSVTKG
                     IDQVIPVDVYVPGCPPRPEALLEGIVLLQERIGKEDMATRWKGDPIVVS"
BASE COUNT          583 a         1094 c         1358 g          577 t
ORIGIN      
        1 ccctcgatgt agatgcacca gtccgggcag ctgcgggcgc agagcatgca gaccgtgcag
       61 ttgtcttcct tgagggcgat gacgccgcgc gcccgggtgg gcggcgtctc cttctcgtgc
      121 gggtactgca cggtggccgc acccttggcc ggcgacggcg gctgcatgaa gcgcttggcg
      181 ccttcgcccg gccagatcgt ctgggtcatc tcgttgaagg tgacgcccat gcccttgagc
      241 aggccgggga gcttcggctt gggaggcttc ttcggcatca gaacgcaacc ttcaagatcg
      301 tggtggcgag gatgttggcc agcgagatcg ggatgagcac ggtccacgcc agcttctgga
      361 gctggtcctc acggaagcgg gggaaggtga agcgcacgaa gaagatgagc ccgccgagga
      421 tcatcatctt ggtgagcatg atgatcggcc cgagcaggtt gaagatggcg ctgtcgaggt
      481 cggcccaggc ggcagggacc gcccagccgc ccaggaagag ggtggcggcc acggcggcga
      541 aggcgccggc cgaggcgaac tcgccgatga agaacagcaa gaagcgcagg cccgagtact
      601 cggtgatgta gccggtgacg agctccgact cggcgatcgg catgtcgaag ggggtctgtc
      661 ccagctcggc ctgcacggcg atcatgaaga tgcagaagcc gatgatctgg gtgaagaagt
      721 acgggttgcc gaggccgtcg aagccgaaga tggcgccgtt ggcctgggcc gagacgatgc
      781 ccgacatgtt gagcgtgccg gcctggatga ccacgccgat gacggcgagg accatgggca
      841 gctcgtaggc gatgagctgg ccggcggcgc gcaggccgcc gatgagcgag tacttgttgg
      901 ccgaagccca gccggcgagc agcacgccga gggcggagat cgacgagacg gccagcacgt
      961 agaagatgcc gccgtcgaag ttggtcacga acgtcatctg cgggccgaac ggcaccgcca
     1021 ggtagacgag cagcacggtg gcgaccacga ggtacggcgc cagcttgaac agcttgcggt
     1081 ccgcacgctc ggggacgatg tcctccttct ggaggaactt gccgacctcg gccagcagct
     1141 gcatggagcc gaacgggccg gcttccatgg gcccgaggcg gctctgcatg aacgacacca
     1201 tcttgaagag gaagaggtag acgaacgtcc ccgccggcag caggaccgcg gcgaggatgc
     1261 cgaccgagcg caggacggtc tcctgccagt aggagagctc gatgccgaag atgggggcgc
     1321 tcaccggtcg atgtctccga ggatgaagta cagggacgcg aggatcgtca cgacgtcggg
     1381 cacgtagacg ccccgggtga cccaggggat gacggcgacg ttgttgaacg aggcggtgcg
     1441 gatcttgacc cggaacgggc cgaggtcgcc cttggagacg acgtagtagc ccatctcgcc
     1501 gagcgggttc tcggtggcca cccaggcttc gccctcgggc accttgatga tgcgcgggac
     1561 cttcgccatg atcggcccgg ccggcaggcc gtcgaggagc tggtcgacga tcttcgtgct
     1621 ctcgcggatc tcctggagcc gcacccagaa ccgggcgaac gagtcgccgt cggggtgggt
     1681 gacgacgttc cagtcgcact cgttccaggc caggccgaca ggctggtccc ggcgcaggtc
     1741 ccagtcgacg ccgctggcgc gcaggttggc accggtcatg ccgtaggcga gggcgacgtc
     1801 ggcggggatg acgccgatgc cgcgggtgcg ggcctggaag atctcgttgc cctcgacgag
     1861 ggtctccatc tcgtcgcaga agtcccggat cttctccatg gaggccttgg tgtcggcgat
     1921 ccagcccttg ggcaggtcgt ccttgaggcc gccgatgcgg tcgaagttgg ggtggaagcg
     1981 gccgccggtg gccgcctcga tctggttgag gacgtactca cggtcgcgga aggcgtagaa
     2041 cgcggcgctg atggcgccga tgtgcagcgc catgtcgccc acgaacagcg agatgttggc
     2101 gatgcgggag agctcgaaga ggaccgtgcg gatccactgg gcccggggcg gggcctcgat
     2161 gtccatgagc ttctcggcgg cgaggatgaa cggcacctcg ttggcgaagc tgcccagcca
     2221 gtcgatgcgg ttgatgagcg tcgtgacctg cgggtaggtc cgcacctcgg tcagcttctc
     2281 gtagccccgg tgcatgtagc ccatgaccgg ctcgcaggag atgacctctt cgccgtcgag
     2341 cttgacgacg atgcgcagcg tgccgtgggt ggcggggtgc tgcgggccca tgttgagggt
     2401 catgccctcg tcggtctcga actcgaggtt cacccggccg tccgcggcct gggcgttggc
     2461 gtaggccagc tgctggcgct cggtgagagc ggtcattccg cggcctcctc ggtctcggct
     2521 tcggcttcgt cgtcggacgc cggcatgggc tcgacgtcga cgatgccggg ccacggcttc
     2581 acgaagcggg ccagcagcgg gaagtccttg cggagcgggt gcccctcgaa ctcgcccggc
     2641 aggtacaggt tctggaggta cgggtgcccg gtgaaggtga tgccgaacat ctcgtgcacc
     2701 tcgcgctcgt gccagttgac cccggcgtac accgggagcc aggtggcgag cgagaggtcg
     2761 tcgtcgggca ggtcgacctt caggttgagg ccgagcttcg acgtgtacga ctgcacccgg
     2821 gcgatgacct ggaaccgggt gtcgccgccg gcgtagccgg tgacgatgtc ggtgttgggc
     2881 tcgggccggg ggtcggtggg ggcgtcttcg ttcttgccgt aggggttcgg cagccagtcg
     2941 atggccgaca ggtactcgaa cgcccggaag ctcaggtggt cgcggaccac gaggccggcg
     3001 gtgcgccagg actcggccgt gacccggacc cacacgtcgt cgccggggcg gatgtggctg
     3061 ccgacgacgg cctcgcccag ctcctcggtg agcttgtcga tgacggcctg gcgggcctcg
     3121 tcgacctcgg gcgcctcggg ggtctcctcc accgtgtcag ctgacgacaa tgggatcacc
     3181 cttccagcgc gtcgccatgt cttccttgcc gatgcgctcc tggagcagca cgatgccctc
     3241 gagcagggcc tcgggccgcg gcgggcagcc gggcacgtag acgtcgacgg ggatgacctg
     3301 gtcgatgccc ttggtgaccg agtagctgtc ccagtagggg ccgccgcagt tggcgcacga
     3361 gcccatggag atgacgtact tcgggtcggg catctggtcg tagaggcggc ggatggccgg
     3421 gaccatcttg tcggtgaccg tgccggagat gcagacgagg tcggcctggc gggggctggc
     3481 cggcagcggg atcacgccga ggcgcatcac gtcgtagcgg ggcgcgccca aggcggcgcc
     3541 catctcgatg gcgcagcagg ccagacccca ctggaagatc cagagggagt acttgcgcga
     3601 ggtgttgagg ag
//
LOCUS       DATMFS010000136         3489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_260031, whole genome shotgun sequence.
ACCESSION   DATMFS010000136 DATMFS010000000
VERSION     DATMFS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260031"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1931)
                     /locus_tag="VNQ33_02545"
     CDS             complement(150..1931)
                     /locus_tag="VNQ33_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97008.1"
                     /translation="MTTSEARPWPALSPLRRYGPLALVVALVAGASVVATAKGKATDT
                     QQRKAPTESVAKSYVDDPKLPVLYSQAKKAGTLDDYDWGDRCDPETGRNAFPSVYAPP
                     CVPVWEGTKPWVDQGGRTITSNGGKTAPGVTADTITIAYYQASPQDISATLQAFGVLD
                     EPKVAQQGVKDLVEAANGMYELYGRKVVFKPFQASGNGKDPATARADAKKVALDMGAF
                     ASIGGPTQTSAYQDELARHHVLCIACGYSTPDSAYQENAPYAWGQLASPDQLLFGVFD
                     FGTKNLFGKKAQFAGDPELAKEDRVFGIVHYEQDPPVFGELQREAVAKYEKQGYAAKT
                     ILTYLLDLNTLPKQAQAIVGKLKAEGVTSVVFLGDPLMPKYLTEQATAQDYHPEWIIT
                     GTVFTDTTAAGRLYDQDQWAHAFGASSLPARTEPQYSEPWRLYRWWYGQDPAAKKTLP
                     LMGPVVSQLFIGLQGAGPDLNAETFAGALFRYPPSGGGPTTPRISFGFHGLFDKPDWV
                     GVDDFTNVWWDAEAKGVDEQDVEGTGMWRYAGGGQRLQLGEVPQLDDDLLFDEAGSVT
                     ILDEVPKEDQTKDYPPWPGSPAAGGGG"
     gene            complement(1928..>3489)
                     /locus_tag="VNQ33_02550"
     CDS             complement(1928..>3489)
                     /locus_tag="VNQ33_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ97009.1"
                     /translation="TAGTIHLGEVDLGALAPHEVPTHGIAQMPGGQGVFPSLTVDENL
                     RVAAWLIRGRRAEVATRVAEVRRRFPILERRAADPAGDLSGGQQQQLALAMALLARPA
                     LLLVDELSLGLAPVVVDQLLQELRALRDDGTTIVIVEQSVNVALTVADHAFFLEKGEV
                     RFSGDAADLLDQPDLVRAVYLQGAREALASGTPARTAPPGTAPGSSAEPGPGAEPGSR
                     GEPGPADARAGIVPPQPASADPVPEPEPVTGTHAAVAVDGLAVAFGGIVAVDHVSFSV
                     APGEVVGLIGPNGAGKTTIFDLISGFTRASAGKVVVLGQDLTDAPASTRARAGLGRSF
                     QDARLFGGLTVAETIAVALERWIQVTDPISEACRLPAVQLTEAAVRERVEELIDLFGL
                     EAFHDKRLSELSTGSRRMVDLACVVAHQPSVVLLDEPSSGIAQREAEALGPVLLGLRD
                     RLGAALVVIEHDISLVSSISDRLVALDQGAVVASGAPAVVLDHPEVVASYLGAGGASL
                     NRSAPLPGGAR"
BASE COUNT          483 a         1196 c         1321 g          489 t
ORIGIN      
        1 tgtgcaaccg ccatcagaac gttcggggag ggcccggacg accaccgtct gggcgcgtgg
       61 gttccggggt tccgcccggg tgcggcggat ggtgatcgat ccccgacgat gcaggagcag
      121 gggcgaaccg aggcgaaccg accgggcggt cagccaccgc ccccggcggc gggggaaccg
      181 ggccagggcg ggtagtcctt ggtctggtcc tccttgggga cctcgtcgag gatcgtgacc
      241 gagccggcct cgtcgaagag caggtcgtcg tcgagctggg gcacctcgcc cagctggagg
      301 cgctggcccc caccggcgta gcgccacatg ccggtgccct cgacgtcctg ctcgtcgaca
      361 cccttcgcct ccgcatccca ccagacgttg gtgaagtcgt cgacgcccac ccagtccggc
      421 ttgtcgaaca ggccgtggaa cccgaagctg atccgaggcg tcgtgggccc gccgcccgag
      481 ggcgggtagc ggaagagcgc accggcgaac gtctcggcgt tcaggtccgg gccagcgcct
      541 tgcaggccga tgaacagctg gctgaccacc gggcccatca gcggcagggt cttcttcgcc
      601 gcggggtcct ggccgtacca ccaccggtag aggcgccagg gctcggagta ctgcggttcg
      661 gtgcgggcag gaaggctcga ggcgccgaac gcgtgggccc actggtcctg gtcgtagaga
      721 cggccggcgg cggtggtgtc ggtgaacacc gtcccggtga tgatccactc ggggtggtag
      781 tcctgcgccg tcgcctgctc cgtcaggtac ttgggcatca acggatcccc gaggaagacc
      841 accgaggtga ccccctcggc cttgagcttc ccgacgatgg cctgggcctg cttcgggagg
      901 gtgttgagat cgagcaggta ggtgaggatc gtcttggccg cgtacccctg cttctcgtac
      961 ttggcgacgg cctcgcgctg gagctccccg aagacgggcg ggtcctgctc gtagtggacg
     1021 atgccgaaga cccggtcctc cttcgccagc tccgggtcac cggcgaactg cgccttcttg
     1081 ccgaacaggt tcttggtgcc gaagtcgaac acgccgaaga ggagctggtc gggactggcg
     1141 agctgccccc acgcgtaggg ggcgttctcc tggtaggcgg aatcgggggt ggagtagccg
     1201 cacgcgatgc acaggacgtg gtggcgggcc agctcgtcct ggtacgccga ggtctgcgtc
     1261 ggccccccga tggaggcgaa cgcgcccatg tcgagggcga ccttcttcgc gtcggcccgg
     1321 gccgtggccg ggtccttgcc gttgcccgac gcctggaacg gcttgaacac gaccttgcgg
     1381 ccgtagagct cgtacatccc gttggccgcc tcgaccaggt ccttcacgcc ctgctgcgcc
     1441 accttcggct cgtcgagcac gccgaacgcc tggagcgtgg cgctgatgtc ctgcggcgag
     1501 gcctggtagt aggcgatggt gatggtgtcc gcggtgacgc ccggggccgt cttccccccg
     1561 ttgctcgtga tggtccgtcc gccctggtcc acccacggct tggtcccctc ccacaccggg
     1621 acgcacggcg gggcgtagac ggacgggaag gcgttgcggc cggtctcggg gtcgcagcgg
     1681 tcgccccagt cgtagtcgtc gagggtcccg gccttcttcg cctgcgagta caggaccggg
     1741 agcttcgggt cgtcgacgta ggacttggcg accgactcgg tgggggcctt gcgctgctgg
     1801 gtgtcggtcg ccttgccctt ggccgtggcc accacgctgg cgccggccac gagggccacc
     1861 accagcgcga gcgggccgta gcggcgaagc ggcgagagcg caggccacgg tcgggcctcg
     1921 gatgtggtca tcgagcgcct ccgggaagcg gagcggagcg gttcaggctt gcacctccgg
     1981 cgcccaggta ggacgcgacc acctcggggt ggtcgagcac caccgccggg gcgccggagg
     2041 cgacgacggc accctggtcg agcgcgacca gtcggtccga gatcgacgag acgaggctga
     2101 tgtcgtgctc gatcacgacg agcgccgctc cgagccggtc gcgcagaccc agcagcaccg
     2161 gcccgagggc ctcggcctca cgctgggcga tgccgctgct gggctcgtcg agcagcacga
     2221 ccgacggctg gtgggccacg acgcacgcca ggtcgaccat gcggcgagac ccggtggaca
     2281 gctcgctcaa ccgcttgtcg tggaaggcct ccaggccgaa caggtcgatg agctcctcga
     2341 cccgctcccg cacggcggcc tcggtgagct gcaccgcggg gagccggcag gcctcgctga
     2401 tcggatcggt cacctggatc caccgctcga gggcgacggc gatggtctcg gccaccgtca
     2461 gcccgccgaa gagccgggcg tcctggaacg agcggccgag cccggcccga gcacgggtgg
     2521 aggcgggggc atcggtcagg tcctgcccga gcaccaccac cttgccggcc gacgcccggg
     2581 tgaagccgga gatcaggtcg aagatcgtgg tcttgccggc gccgttcggg ccgatgagcc
     2641 cgaccacctc gccaggggcg accgagaacg agacgtggtc gacggcgacg atgccgccga
     2701 acgccacggc cagcccgtcg acggccaccg cggcgtgggt gccggtgacg ggctcgggct
     2761 cgggaacggg gtcggcggac gccggctgcg gtggcacgat cccggctcgg gcgtcggccg
     2821 gtccgggttc cccgcgggag ccgggctccg cacccgggcc cggctccgcg ctcgatccgg
     2881 gcgccgtgcc aggcggggcc gtccgagcgg gggtgccgga ggcgagggcc tcgcgggcgc
     2941 cctggaggta gacggcgcgg accaggtcgg gctggtcgag gaggtcagcc gcatcgccgg
     3001 agaagcggac ctcgcccttc tccaggaaga acgcgtgatc ggccacggtg agggcgacgt
     3061 tcaccgactg ctcgacgatc acgatcgtgg tgccgtcgtc gcgcagggcc cgcagctcct
     3121 ggagcagttg gtcgacgacg acgggggcca gaccgagcga gagctcgtcg accagcagca
     3181 gcgccggccg ggccagcagg gccatggcca aggcgagctg ctgctgctgg ccacccgaca
     3241 ggtccccggc cggatcggcg gcccggcgct cgaggatcgg gaaccggcgg cgcacctcgg
     3301 cgacgcgggt ggccacctcg gcgcgccggc cccggatgag ccaggccgcg acccgcaggt
     3361 tctcgtcgac ggtgagcgac gggaacacgc cttggccacc gggcatctgg gcgatgccgt
     3421 gggtggggac ctcgtgcggg gcgagcgcgc cgaggtccac ctcgcccagg tggatggtcc
     3481 ccgccgtga
//
LOCUS       DATMFS010000137         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_5914, whole genome shotgun sequence.
ACCESSION   DATMFS010000137 DATMFS010000000
VERSION     DATMFS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5914"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VNQ33_02555"
     CDS             complement(<1..229)
                     /locus_tag="VNQ33_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWJ97010.1"
                     /translation="MAALDGYVEGTFTFEGKERTVFRRGEGPAVLVISEIPGITPLVA
                     DFGRRVAEAGFTAVLPSLFGTPGKDPSSAYAA"
     gene            complement(249..>2198)
                     /locus_tag="VNQ33_02560"
     CDS             complement(249..>2198)
                     /locus_tag="VNQ33_02560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ97011.1"
                     /translation="LGLVRFAADAFLGALRRRSVALLADGQAEVFELIARGAPVATAI
                     LAARALLARHTLGATVVIATVEEGGRLSLVTEPDDPDEEALAAWFAGLDPGLGNPFGQ
                     AVITGEPVMVANAYGDPRYSEQVLPDDTCQSATILPVRSPRNGQTLAIVGLLGAESGA
                     PIARPAVRDSVLSLVTVAVERSIDERRLAHQATHDPLTGVGNRAALLDRLTLALARAK
                     RSGRNVAVLFCDLDGFKAVNDQYGHDRGDRLLVEVAERIGRAVRPSDTVCRTGGDEFV
                     IVCEDLNDADQAHTIAERVRTAIESEPIDVGETRLPCTVSVGVALADLPADDPDRVLR
                     TADLAMYARKQHRAAVRRHPASREETARAEDERELGSVERAALERSGAIGPVSAPVRR
                     TDPFGLDLAEAIEHDGLHLVHQPLVGRDGSLTGIEALVRWEHPVLGPVGPVRILSAAA
                     NDDLAGPLGRWVRRAALAERRRWLGHLDPARAVPVHLNVADSELALPHLVDSVLADVD
                     EAGLDVGDIVVEVRERHLADAELRSVVGELADAGIAVLVENVGQGGLSLSDLAGLPVG
                     GLKLGQSLVARIREDDPAGVEVARSLVMLAHGLGWRSLAVGVETDHQRAVLFGFGIDA
                     VQGQVAAMPMPGDELLAWLDRHPSA"
BASE COUNT          299 a          783 c          851 g          265 t
ORIGIN      
        1 gggcggcgta ggcgctggag gggtccttgc ccggcgttcc gaacagcgac ggcagcaccg
       61 cggtgaaccc tgcctcggcg acgcgccgtc cgaagtcggc gacgagcggt gtgatgccgg
      121 ggatctcgga gatgacgagg accgccgggc cctcgccacg ccggaacacc gtccgctcct
      181 tgccctcgaa cgtgaaggtg ccttcgacgt acccgtccag tgcggccatc tccgaacgct
      241 acgcagcgtc aggcgctcgg gtggcgatct agccaggcca ggagctcgtc gccgggcatc
      301 ggcatggcgg ccacctggcc ctggacggca tcgatgccga agccgaagag cacggcgcgc
      361 tggtggtcgg tctcgacgcc gaccgccaag ctccgccacc ccaggccgtg ggccagcatc
      421 acgagcgacc gagcgacctc gacgccggca ggatcgtctt cgcggatgcg ggccacgagg
      481 gactggccca gcttgagccc gccgaccggc agcccggcga ggtcggacag cgacaggccg
      541 ccctgcccga cgttctccac gagcacggcg atccccgcgt cggccagctc acccaccacg
      601 gaccgcagct ccgcgtccgc gaggtgccgc tcgcgcacct cgacgacgat gtcgccgacg
      661 tccaggccgg cctcgtcgac gtcggcgagc acgctgtcga cgaggtgcgg gagcgccagc
      721 tcggaatcgg cgacgttcag gtgcaccggc acggcgcgcg ccggatccag gtggccgagc
      781 cagcggcggc gctcggcgag agcagcgcgg cggacccacc ggcccagcgg gccagcgagg
      841 tcgtcgttgg ccgcggccga caggatccgc accgggccga ccgggccgag cacggggtgc
      901 tcccaccgca ccagggcctc gatcccggtg agcgatccgt cgcgcccgac gagcggctgg
      961 tggacgaggt gcaggccgtc gtgctcgatc gcctcggcga ggtcgaggcc gaacgggtcg
     1021 gtccggcgca cgggcgccga gaccggtccg atggcgccgc tgcgctccag cgcggcgcgc
     1081 tcgacgctgc cgagctcccg ctcgtcttcc gctcgcgcgg tctcctctcg gctggcggga
     1141 tggcgccgga cggcagcacg gtgctgcttg cgggcgtaca tggcgaggtc cgccgtgcgc
     1201 agcacccggt ccgggtcgtc ggcgggcagg tcggcgaggg cgaccccgac gctcaccgtg
     1261 cacgggagcc tggtctcgcc cacgtcgatg ggctcgctct cgatggcggt gcgcacgcgc
     1321 tcggcgatgg tgtgggcctg gtccgcgtcg ttcaggtcct cgcagacgat cacgaactcg
     1381 tcgccgccgg tgcggcacac cgtgtccgag ggccgcacgg cgcgcccgat gcgctcggcc
     1441 acctcgacga ggaggcggtc gccgcggtcg tggccgtact ggtcgttcac ggccttgaag
     1501 ccgtcgaggt cgcagaacaa gacggccacg ttgcgcccgg agcgcttcgc ccgggcgagg
     1561 gccaaggtga ggcggtcgag cagcgcggcc cggttcccga ccccggtgag ggggtcgtgg
     1621 gtcgcctggt gggccaggcg gcgctcatcg atcgatcgct ccacggcgac ggtcacgagg
     1681 gacagcaccg agtcgcgcac cgccggccgg gcgatggggg caccggactc ggccccgagc
     1741 aggccgacga tggcgagggt ctggccgttg cgcggcgacc gcacggggag gatggtcgcg
     1801 gactggcagg tgtcgtcggg caggacttgc tcggagtacc gggggtcgcc gtaggcgttg
     1861 gccaccatca ccggctcgcc ggtgatgacc gcctggccga aggggttgcc gagacccgga
     1921 tcgagaccgg cgaaccaggc ggcgagggct tcctcgtcgg ggtcgtccgg ctcggtcacc
     1981 agcgacaggc gaccgccctc ctcgaccgtc gcgatgacga ccgtggcgcc gagcgtgtga
     2041 cgggcgagca gggcgcgggc ggccaggatc gcggtggcga ccggggcacc gcgggcgatg
     2101 agctcgaaca cctcggcctg gccatcggcg agcagcgcga cgctgcggcg ccggagcgcg
     2161 ccgaggaagg cgtcggcggc gaagcggacc agcccgag
//
LOCUS       DATMFS010000138         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_167834, whole genome shotgun sequence.
ACCESSION   DATMFS010000138 DATMFS010000000
VERSION     DATMFS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167834"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VNQ33_02565"
     CDS             <1..315
                     /locus_tag="VNQ33_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97012.1"
                     /translation="VREGWGMTLTEAAACGTPAVATRIPGHVDALAEGTSGLLADDPA
                     DLARQIARIATDAPLRERLTAGALQHAGHFTWDATATKIMEILVDEALGRPGLRRPGA
                     TG"
     gene            312..>2666
                     /locus_tag="VNQ33_02570"
     CDS             312..>2666
                     /locus_tag="VNQ33_02570"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HWJ97013.1"
                     /translation="MRPALRQRLREALPTAILAVTIYVPLLLTKPGRVGADTKTYLYL
                     DPGRMLGRATSMWDPNIGMGTVTHQNIGYLWPIGPWYWFADVLGMPDWVAQRLWLGTI
                     LFAAGVGVRFMLKALGQEGPHVTAATFVYALTPYVLSLAARLSVILLPYAGLPWMIGL
                     AVLSLRKGGWRYPALFGLVVATIGSVNATALILAGVGPVLWIAHEVIITREARLRDAA
                     KTIGRIGVMTVGCSLWWLAGLWAQGGYGIDILRYTETAETVATASLSLEVLRGLGYWF
                     FYGEDRFGPWIAPSRSYTQRVDILLLTYVLPVLGLIGASVTRFRERTFFLLLMVVGLF
                     LAVGAHPWDDPSPAGSAVKAFLESDAGLAMRSLPRAAPLVVLAMAVFVGSLVSAVSRG
                     HERWARPLTAGVVVLAILSLPPLWRGQMVDSNLDRAEDIPDYWVEAAAAMDARDDGTR
                     VLEIPGEDFASYRWGTTVDPITPGLMDRPYVARELIPYGSPPSANVLNAVDRRLQEQV
                     AEPGSFAPMARFLGVGDISVRSDLTYERYNTPRPRRLWATIQAAPDLGKPIGFGGTAR
                     NQARPNLPLIDEQELLTDPDAEDPPQVAIVSVKDPEKIVRTAPVDQPIILAGDGEGIV
                     DLAASGLVTGHELLFYSGTFAGDPAALRSKLDDDAVLVLTDTNRREARRWGTVRDNTG
                     VTERPGQEPVKADPKDQRLELFPSAGDDSSTVTDVQGGVWAEATGYGNSVSLTPEDDP
                     ANAVDGDHGTAWRIGGFSSATGETLRLRYRDPVTTDHLTLLQAIG"
BASE COUNT          346 a         1048 c          886 g          386 t
ORIGIN      
        1 gtccgggagg gctggggcat gacgctgacc gaggccgccg cctgtggcac cccggccgtc
       61 gccacgcgca tccccggcca cgtcgacgcc ctcgccgagg gcacgagcgg cctcctcgcc
      121 gacgacccgg ccgacctggc ccgccagatc gcccgcatcg ccaccgacgc gccgctgcgc
      181 gagcgcctca ccgccggtgc gctccagcac gccggccact tcacctggga cgccaccgcc
      241 accaagatca tggagatcct ggtcgacgag gcgctgggcc ggcccgggct ccggcgccct
      301 ggcgcgacgg gttgaggccg gcgctccggc agcgcctccg ggaggcgctg cccacggcga
      361 tcctggccgt cacgatctac gtcccgctgc tgctcaccaa gccaggacgc gtcggcgcgg
      421 acaccaagac gtacctctac ctcgatcccg ggcgcatgct cgggcgggcc accagcatgt
      481 gggaccccaa catcgggatg ggcacggtca cccaccagaa catcggctac ctgtggccga
      541 tcgggccctg gtactggttc gccgacgtgt tgggcatgcc cgactgggtc gcccagcgcc
      601 tgtggctcgg gacgatcctc ttcgccgccg gcgtcggcgt gcggttcatg ctcaaggccc
      661 tcggccagga gggcccgcac gtcaccgcgg ccaccttcgt ctacgccctg acgccctacg
      721 tgctctcgct ggcggctcgc ctctcggtga tcctgctccc ctacgccggg ctgccgtgga
      781 tgatcggcct cgccgtcctc tcgctccgca agggaggctg gcggtacccc gcgctcttcg
      841 ggctggtcgt ggccaccatc gggagcgtca acgcgaccgc gctcatcctc gcgggcgtcg
      901 gccccgtcct ctggatcgcc cacgaggtga tcatcacccg cgaggcgcgc ctgcgcgacg
      961 ccgccaagac catcgggcgc atcggcgtca tgacggtggg ctgctcgctg tggtggctcg
     1021 ccgggctctg ggcccagggc ggctacggga tcgacatcct ccggtacacg gagacggcgg
     1081 agacggtggc caccgcctcg ctgtcgctcg aggtgctgcg gggcctcggc tactggttct
     1141 tctacggcga ggaccgcttc gggccgtgga tcgcgccctc tcgctcctac acgcagcggg
     1201 tcgacatctt gctgctgacc tacgtcctgc cggtcctggg gctcatcggc gcgtcggtca
     1261 cccgcttccg ggagcggacc ttcttcctcc tgctcatggt cgtcggcctc ttcctcgcgg
     1321 tcggcgctca cccgtgggac gacccgtcgc cagcgggctc ggcggtcaag gcgttcctcg
     1381 agtccgacgc cggtctggcc atgcggagcc ttccccgcgc cgcacccctg gtcgtgctcg
     1441 ccatggccgt gttcgtcggg tcgctcgtct cggcggtctc gcgcggccac gagcgctggg
     1501 cccgcccgct caccgccggt gtcgtcgtct tggcgatcct cagcctgccg ccgctgtggc
     1561 ggggccagat ggtcgacagc aacctcgacc gcgccgagga catccccgac tactgggtgg
     1621 aagccgccgc cgccatggac gcacgcgacg acggcacccg ggtgctggag atcccgggcg
     1681 aggacttcgc gtcgtaccgc tggggcacca ccgtcgatcc gatcaccccc gggctcatgg
     1741 atcggcccta cgtcgcccga gagctcatcc cgtacggttc gcccccctcg gcgaacgtgc
     1801 tgaacgccgt cgaccgccgg ctgcaggagc aggtggccga gcctggctcc ttcgccccga
     1861 tggcacggtt cttgggcgtg ggcgacatct cggtgcgctc cgacctcacg tacgagcggt
     1921 acaacacgcc tcgtccccgg cggctgtggg ccacgatcca ggcggcgccg gacctgggca
     1981 agcccatcgg cttcggcggc acggcccgca accaggcccg gcccaacctg ccgctcatcg
     2041 acgagcagga gctcctcacc gatcccgacg ccgaggatcc gccccaggtc gccatcgtct
     2101 cggtgaagga cccggagaag atcgtccgca ccgcgccggt cgaccagccg atcatcctgg
     2161 cgggcgacgg cgagggcatc gtggacctgg ccgcgtcggg gttggtcacc ggccacgagc
     2221 tgctcttcta ctccggcacg ttcgcgggcg accccgccgc cctgcgctcg aagctcgacg
     2281 acgacgccgt gctcgtgctc accgacacca accgccggga ggcccgccgc tggggcacgg
     2341 tccgcgacaa caccggcgtg accgagcgac cgggccaaga gcccgtcaag gccgacccga
     2401 aggaccagcg cctcgagctc ttccccagcg ccggcgacga ctcctccacc gtcaccgacg
     2461 tgcagggcgg ggtgtgggcc gaagccaccg gctacggcaa ctcggtctcg ctcacccccg
     2521 aggacgaccc cgccaacgcg gtcgacggcg accacggcac ggcctggcgg atcggcggat
     2581 tcagctccgc gacgggcgag acgctccggc tccggtaccg cgacccggtc accaccgacc
     2641 acctgaccct cctccaggcc atcggc
//
LOCUS       DATMFS010000139         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_98604, whole genome shotgun sequence.
ACCESSION   DATMFS010000139 DATMFS010000000
VERSION     DATMFS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98604"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VNQ33_02575"
     CDS             complement(<1..1439)
                     /locus_tag="VNQ33_02575"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWJ97014.1"
                     /translation="MSDATEGAGATIEDYLLEERTFPPSEAFKEHALVASNHLHEEAD
                     RDYQGFWARQALDLLDWQQEWDTICDWQLPYARWFVGGKLNVSENCLDRHVRAGLGDR
                     VAFHWEGEPGDTRTITYAELLGEVERFANVLKGLGVAKGDRVAIYLPMIPEAVVAMLG
                     CTRVGAPHSVVFGGFSADSLADRINDAECKVLVTADGGWRRGKPGLLKPTADVAVANT
                     PSIEHVVVVDRIASSGGEASIDWVDGRDHWYHELMAAADPSCPPEPMDSEDLLYLLYT
                     SGTTGKPKGIMHTTGGYLTQAAFTHKYVFDLHADEDVYWCTADVGWVTGHSYIVYGPL
                     ANGATSVMYEGTPDTPGKDRFWGLVEKYGVTVLYTAPTAIRTFMKWGRQEPERHDLSS
                     LRVLGSVGEPINPEAWIWYHQVIGGERCPIVDTWWQTETGAIMISPLPGATTTKPGSA
                     TFPLPGIGAELVDDAGQPVERGGGYLTLTR"
     gene            1573..2088
                     /locus_tag="VNQ33_02580"
     CDS             1573..2088
                     /locus_tag="VNQ33_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HWJ97015.1"
                     /translation="MTSVNPTDATDAPDREELTWERFGTATRALAETIAADAWRPDIV
                     VAVARGGLTVAGALAYALGVKNCGTINVEFYTGVDERLDVPVVLPPSLQLVDVTGLQV
                     LVVDDVADTGHTLRLVREVLAQHVEECRTAVLYHKSHSVIVPDYCWKPTDAWINFPWS
                     VLPPVAGLPPR"
     gene            complement(2223..>2481)
                     /locus_tag="VNQ33_02585"
     CDS             complement(2223..>2481)
                     /locus_tag="VNQ33_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97016.1"
                     /translation="GRDQGGLVQLTKDAGLDLGDDLLRLASDGCEPDKTDPADAFAAI
                     DHFSVAFLRYELGIDPEPVGLDQATADAFTTAAVTLTSAPR"
BASE COUNT          331 a          872 c          892 g          386 t
ORIGIN      
        1 cgggtgagtg tgaggtagcc gccgccgcgc tcgacgggct ggccggcgtc gtccaccagt
       61 tcggcgccga tgcccgggag cgggaacgtg gccgagccgg gcttggtggt ggtggcaccg
      121 gggagcggcg agatcatgat cgccccggtc tcggtctgcc accacgtgtc gacgatcggg
      181 cagcgctcgc cgccgatgac ctggtggtac cagatccacg cctcggggtt gatgggctcg
      241 cccaccgacc cgaggacccg cagcgaggag aggtcgtggc gctccggctc ctggcggccc
      301 cacttcatga aggtccggat ggccgtgggc gccgtgtaga ggacggtcac cccgtacttc
      361 tcgacgaggc cccagaaccg gtccttgccg ggcgtgtcgg gcgtgccctc gtacatcacc
      421 gacgtggccc cgttggcgag cggcccgtag acgatgtagc tgtggccggt gacccagccg
      481 acgtcggccg tgcaccagta gacgtcctcg tcggcgtgca ggtcgaagac gtacttgtgg
      541 gtgaacgcag cctgggtcag gtagccgccg gtggtgtgca tgatgccctt gggcttgccc
      601 gtggtgcccg acgtgtacag caggtacagc aggtcctcgc tgtccatcgg ctcgggcggg
      661 caggaggggt cggctgctgc catgagctcg tggtaccagt ggtcccggcc gtcgacccag
      721 tcgatcgagg cttcgccgcc cgacgacgcg atgcggtcga cgacgaccac gtgctcgatg
      781 gacggggtgt tggcgacggc gacgtcggcc gtcggcttca gcagcccggg cttgccccgg
      841 cgccacccgc cgtcggccgt caccagcacc ttgcactcgg cgtcgttgat gcggtcggcc
      901 agcgagtcgg ccgagaaccc gccgaacacg accgagtggg gagcgccgac gcgggtgcac
      961 cccaacatgg cgaccacggc ctcggggatc atcggcaggt agatggcgac gcggtcgccc
     1021 ttggccacgc cgaggccctt gaggacgttg gcgaagcgct cgacttcgcc cagcagctcg
     1081 gcgtaggtga tggtgcgggt gtcgccgggc tcgccctccc agtggaacgc cacccgatcg
     1141 ccgaggccgg cgcggacgtg gcggtccagg cagttctcgc tcacgttgag cttcccgccg
     1201 acgaaccacc tggcgtaggg gagctgccag tcgcagatcg tgtcccactc ctgctgccag
     1261 tccaggaggt ccagcgcctg gcgggcccag aagccctggt agtcccggtc cgcctcctcg
     1321 tgcaggtggt tggaggcgac cagcgcgtgc tccttgaacg cctccgacgg cgggaacgtc
     1381 cgctcctcca gcaggtagtc ctcgatcgtg gcgcctgcgc cctcggtggc gtcgctcatg
     1441 gctggtgctc cgttcccctg aaccggtgcg gcacgcggcc gcagtgcgtc ggtcacccta
     1501 gcgacgcccg ttcccgtggt ggctggagcc tgggggatca cggcccctcg gggcgcgcga
     1561 agctacctgt ccatgacgag cgtgaacccg acggatgcga ccgatgcccc cgatcgcgag
     1621 gagctcacct gggagcgctt cgggacggcc acccgcgccc tcgccgagac catcgccgcc
     1681 gatgcgtggc ggcccgacat cgtcgtcgcc gtcgcccgcg gcgggctgac cgtcgccggc
     1741 gccctcgcct acgccctcgg cgtgaagaac tgcgggacga tcaacgtcga gttctacacg
     1801 ggcgtcgacg agcggctcga cgtgcccgtc gtcctcccgc ccagcctcca gctggtcgac
     1861 gtcaccggcc tccaggtcct cgtcgtcgac gacgtcgccg acaccggcca caccctccgc
     1921 ctcgtgcgcg aggtcctggc ccagcacgtc gaggagtgcc gcaccgcggt gctgtaccac
     1981 aagagccact cggtgatcgt ccccgactac tgctggaagc ccaccgacgc gtggatcaac
     2041 ttcccctggt cggtcctccc gcctgtcgcc ggcctccctc cgcgctgacc gccgcctttc
     2101 gggtcacctg ggcacccaga gggtgcacgt gtgacccgaa accgcggtgg gccggcaccc
     2161 acgtccattt cgggtcacct gggcacccgg gaggtgcacg cgtgacccga aatccgtctg
     2221 ggttagcggg gggcgctggt gagggtgacg gcggcggtgg tgaaggcgtc ggcggtggcc
     2281 tggtccaggc cgacgggctc ggggtcgatg ccgagctcgt agcggaggaa ggccacgctg
     2341 aagtggtcga tggcggcgaa ggcgtcggcc gggtcggtct tgtcgggctc gcagccatcg
     2401 ctcgccaacc ggaggaggtc gtcgcccagg tccaagccgg cgtccttggt gagctgcacc
     2461 aggccgccct ggtcccggcc g
//
LOCUS       DATMFS010000140         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_260125, whole genome shotgun sequence.
ACCESSION   DATMFS010000140 DATMFS010000000
VERSION     DATMFS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260125"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..>2912)
                     /locus_tag="VNQ33_02590"
     CDS             complement(361..>2912)
                     /locus_tag="VNQ33_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWJ97017.1"
                     /translation="RLLDALDETPLARITYLAAPAGYGKTCLVQRWLDRRSAPGDVLV
                     RSDGSPEELVRAILRALAPKGLVDHIEHLVTDAAGLVDGTGRLHSGGRAAVASLVEAR
                     PDGCVVVDIAGDHPPLPVLADLAAIFVAAPGGQVRFMVAARARPHPALARLQAEGHLR
                     VLDQSVLAMTRHETEQMLAADRDAPLPPEIVDRIVTAAEGWPAAVVIAARTATAEPGD
                     QDDHHLGRVLDLTRRYLASEVLDGLTDDELRAAAAASIFDEVPSALVAEICGMPSASA
                     TLWGLRERSCFLRTTRRGTWLRFHPLMRRALAQHCRELDLTEHALRTAHQWFRDHRYM
                     VEALRCLIRLCDWRAAVELSSVRMADLITADNLEEFVDLLEQIPSWAADETPQILGIA
                     AMLELGLARPGRAAAFITQAERHPELVRFCLVLRGTASPYQSNMQPLLQAGEEAMARV
                     HGSTDEDYHNYLHRPKTSHYLMVVQAGLLAAAAYSDEWDRVAMHQRPIDPIAAAEVPP
                     YARLVIAHRHMTYHALAGHTAELLALADQVRRLRDDHPDVYPLNPGLMEITTAEVLRL
                     QGREDEAASVLDGIDPQRPLYPWLNHAGWLIGLRAHLAVDGHRPRQAHELIEREQEVL
                     GAKPAPAIEAMIAAAAARAHLALAQPGAALAVLAAAPPTGWTRWVAALTTLTQADLPG
                     ALAIVDGWPPERAPISETRRQLAIAATAHATGDATRRDAALCSASRLADAYGFVQPVV
                     ELGPRWAGLWRHGPETGAATLRALDKLSNDATTDDGVLTPSEMAVMAHLCEDVALSQI
                     ASALHLSINTVKSHVKSIYRKLGVNSRAEAIDRWRSGPGKRSEHTHRARPS"
BASE COUNT          396 a         1009 c         1102 g          405 t
ORIGIN      
        1 ttcgtgctgt accggcgccg cgacacggcc gtggcctgac ccccagacgg tcggtcgcag
       61 gttggctggg tgacgtcgag ctggtaatcg gctcgaccga ccccggaggt accaccacca
      121 cgccgatgac cggtccgggt cctccgtgat tcccgcctca cggaggaccc ggccggtcat
      181 cggtgcaccg aggccagggc gtcgaacgct cggccccacc ggtaccagct ggtagccggc
      241 ccatcctcgg cgaccgacgg tctggacgag gcgaccccca agcgcaccac gcttgggggt
      301 cgcttcgttt tccggtcagg agcgggtccg gcggccgggc cagcagaacc ggcccggcga
      361 tcagctcggg cgggctcggt gcgtgtgctc ggaccgcttg ccggggcccg atcgccagcg
      421 atcgatcgcc tcggcccggg agttgacccc gagcttgcgg tagatgctct tcacatggga
      481 cttgaccgtg ttgatcgaga ggtggagcgc cgaggcgatc tgcgagagcg cgacgtcctc
      541 gcacaggtgc gccatcaccg ccatctcgct cggggtcaac acgccgtcat cggtggtggc
      601 gtcgttcgac agcttgtcga gcgcacggag cgtcgccgcg cctgtctcgg ggccgtggcg
      661 ccacagcccg gcccaccgcg ggccgagctc gacgaccggc tgcacgaagc cgtaggcatc
      721 ggcaagcctc gacgcgctgc agagggcggc atcgcgccgg gtcgcgtcgc cggtggcgtg
      781 ggccgtggcg gcgatcgcca actggcgacg ggtctcgctg atcggggcgc gctcaggcgg
      841 ccacccatcg acgatggcga gcgcgccggg cagatcggcc tgggtcagcg tcgtcagggc
      901 cgccacccag cgggtccacc cggtgggtgg cgctgccgcc agcaccgcca gcgccgcgcc
      961 gggctgggcc agcgcgaggt gggcgcgagc cgcggccgcg gcgatcatgg cctcgatggc
     1021 cggtgccggc ttcgctccga gcacctcctg ctcgcgctcg atgagctcgt gggcctggcg
     1081 agggcggtgt ccgtcgacgg cgaggtgggc gcgcagtccg atcagccatc ctgcgtggtt
     1141 cagccacggg tacagcggac gctgaggatc gatgccgtcg aggaccgatg ccgcctcgtc
     1201 ctcgcggccc tgcaggcgca ggacctcggc cgtcgtgatc tccatcagcc ccgggttgag
     1261 cggatagacg tcgggatggt cgtcgcggag gcggcgaacc tggtcggcca gcgccagcaa
     1321 ctcggcggtg tggccggcca aggcgtggta cgtcatgtgc cggtgggcga tgaccagccg
     1381 ggcatagggc gggacctcgg cggcggcgat ggggtcgatg gggcgctggt gcatcgccac
     1441 gcggtcccac tcgtcgctgt aggccgcggc agccagcagc cccgcctgca ccaccatcag
     1501 gtagtgcgag gtcttcggcc ggtggaggta gttgtggtag tcctcgtcgg tgctcccgtg
     1561 caccctcgcc atcgcctctt ccccggcttg gagcagcggc tgcatgttgc tctggtacgg
     1621 cgaggcggtg cctcggagga ccaagcagaa ccgcacgagc tccgggtgcc gctcggcctg
     1681 ggtgatgaac gccgccgccc ttcccggccg agccagaccc agctcgagca tcgccgcgat
     1741 gcccaggatc tgcggggtct cgtcggccgc ccagctcggg atctgctcga ggaggtcgac
     1801 gaactcctcg aggttgtcgg cggtgatcag gtcggccatg cgcacagacg agagctcgac
     1861 cgcggcgcgc cagtcgcaga gccggatgag gcaccgcagc gcctcgacca tgtaccggtg
     1921 gtcccggaac cactggtgcg ccgttcggag cgcgtgctcg gtgaggtcga gctcccggca
     1981 gtgctgggcc agcgcgcggc gcatgagcgg gtggaaccgc agccacgtcc cccggcgggt
     2041 ggtgcggagg aagcacgacc gctcgcggag gccccagagc gtggccgaag cggacggcat
     2101 gccgcagatc tccgccacga gcgcggacgg cacctcgtcg aagatgctcg ccgcggccgc
     2161 ggcacggagc tcgtcgtcgg tcaacccgtc gaggacctcc gaggcgaggt agcggcgggt
     2221 gaggtccaag acgcgcccga ggtggtggtc gtcttggtcg ccgggctccg ccgtcgccgt
     2281 gcgcgccgcg atcacgaccg cagccggcca gccctcagcg gccgtcacga tccgatcgac
     2341 gatctcgggt ggcaggggcg cgtcgcgatc ggcggcgagc atctgctcgg tctcgtgcct
     2401 ggtcatggcc aggacggact ggtccaagac ccgcaggtgt ccctccgctt ggaggcgggc
     2461 gagcgccgga tgaggccgcg cccgagccgc caccatgaag cggacctggc cgccgggtgc
     2521 tgcgacgaag atcgcggcca agtcggcgag gaccggcagc ggagggtggt cgccggcgat
     2581 gtcgacgacc acgcagccgt ccggcctggc ctccacgagc gaggcgaccg cggcgcgacc
     2641 gccgctgtgc aaccggccgg tgccatcgac gaggccggcg gcgtcggtga ccagatgctc
     2701 gatgtggtcg acgagtccct tcggcgccag ggcccgcagg atggcccgca ccagctcctc
     2761 gggggacccg tcggagcgaa cgaggacgtc gccgggcgct gagcgccgat ccagccaccg
     2821 ttgcacgagg cacgtcttgc cgtagccggc gggcgcagcc aggtaggtga tgcgcgccag
     2881 cggcgtttcg tcgagcgcgt cgaggagccg ct
//
LOCUS       DATMFS010000141         7056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_145396, whole genome shotgun sequence.
ACCESSION   DATMFS010000141 DATMFS010000000
VERSION     DATMFS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145396"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="nhaA"
                     /locus_tag="VNQ33_02595"
     CDS             <1..1121
                     /gene="nhaA"
                     /locus_tag="VNQ33_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWJ97018.1"
                     /translation="SFAIDESLQHVVNDGLMALFFFVVGLEIKRELVAGELRDRRVAV
                     LPAVAALGGMVVPAGIYVTLNTGGHGSHGWGIPMATDIAFALGVVALLGRRVPAPLKV
                     FLLTLAIVDDIGAIVVIAVFYSDGIRWTWFIEALVVLGLVVLAQRARVRSLVVYVALG
                     VAAWLFVFESGVHATIAGVVLGLLTPAKSLLPEVEAEAIVDQLEGRTDLTAADVHRVE
                     FLVRESVPPTERIEHLLHPWTSYVIVPLFAFANAGIVLTGDAVTSPSSVLVGVVLGLV
                     LGKTVGISLFSWIAVKLGWGVLPDGVRFGQLVGVAALGGIGFTVSLFITALAFEGEPL
                     LADQAKIGILVASLAAAALGSVILTLAARRSISGHPGP"
     gene            complement(1227..2465)
                     /locus_tag="VNQ33_02600"
     CDS             complement(1227..2465)
                     /locus_tag="VNQ33_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97019.1"
                     /translation="MDFSYSPKAEALRTELLDFMDSHVYPAEAVYHQQIVDSGDPHTH
                     PPVMEELKQEARKRGLWNLFLPHKTQWTDGLSNSDYAPLAEIMGRSHIASQACNCSAP
                     DTGNMELLTMFGTPEQQEEWLYPLLGGQIRSAFAMTEPAVASSDATNISCRIERDGDE
                     YVINGRKWWISGAADERCQLFILMGKTDPDAARHVQQSMILVPRDAPGLEIVRALPVF
                     GYQDQEGHCEIQFTDVRVPASNLLGTEGSGFALAQARLGPGRIHHCMRCIGAAERALE
                     LMCQRVTDRVAFGRPLSEQGVIREWIADSRMEIEQARLLTLKAAYLMDTVGNKGAAIE
                     ISAIKVVVPNMALRVIDRAIQAHGGGGVSDDFPLASMYAGIRTLRLADGPDEVHRRQV
                     AKRELGRYTQTEIPPDVARV"
     gene            complement(2769..3530)
                     /locus_tag="VNQ33_02605"
     CDS             complement(2769..3530)
                     /locus_tag="VNQ33_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97020.1"
                     /translation="MTTPSRFDGRTALVTGAASGIGKATALRLAADGAAVGCLDMAES
                     VHETAAEILAAGGQAVAGTVDVSDPDSVAAAVATIEGDLGPTRILANVAGVLRFGITH
                     ESSVEDFDLQIAVNLKGPFLMARAVIPSMLANGGGAITTVASSAGVFGQAYMPGYAAS
                     KGGVAMLSRAIAWEYQKLGIRSNAIAPGGVETNMTSAVTFPEGIDFDLVKKTAAPGYR
                     MMQAEEPAALIAFLSSDEATAINGTVIPIDFGITA"
     gene            3682..4098
                     /gene="arfB"
                     /locus_tag="VNQ33_02610"
     CDS             3682..4098
                     /gene="arfB"
                     /locus_tag="VNQ33_02610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWJ97021.1"
                     /translation="MADDDLPVSRGVRIPRRELVVSFRASGGPGGQHANKTATRVELV
                     YDVEHSTALLPVQRERVMGKLGPVVRIVVDDERSQLRNRAIAEERLVARLSQALHVEA
                     PRRKTKPSKGAKERRLQGKKQRSETKANRRPPRHDA"
     gene            complement(4206..4832)
                     /locus_tag="VNQ33_02615"
     CDS             complement(4206..4832)
                     /locus_tag="VNQ33_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97022.1"
                     /translation="MEADIGGVLASPAVSLTDALAVAASVVFLVRLLPQPIRLARSGV
                     AAGVSSAAALNAVVSAVAWIAYGLVHGLPVVWGVSVLALVPCTWQAVLLRHQVTRRDL
                     AAVAGFVAALLVAAALGVLGVALAGTVLVTAGPQVRRVLREHDLSGVAPATWWVAVVD
                     AVTWGAYGWAVSDAALVGYFFALLAAAVIVLSRLAWTSRVPSPAAPAV"
     gene            complement(4976..5458)
                     /locus_tag="VNQ33_02620"
     CDS             complement(4976..5458)
                     /locus_tag="VNQ33_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97023.1"
                     /translation="MSTVLTPSTRSQRDRLLRLGVMIFVASLPIAGILLALVNWQVSL
                     IFVVVWTVGLLFGVGQQRTVSLKVDANGLQYEAGTFVLRTSWADLDRIGEATLPSGKT
                     QAVVLKKGGLRWAHTAATRAQVQQRGWDRIIPIDDFAPKWPAGKLADAIREHRPDLLP
                     "
     gene            5575..>7056
                     /gene="topA"
                     /locus_tag="VNQ33_02625"
     CDS             5575..>7056
                     /gene="topA"
                     /locus_tag="VNQ33_02625"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWJ97024.1"
                     /translation="MPKPLVIVESPAKARTIAGFLGNDFNVESSVGHIRDLPSSGLNI
                     DVDHHFEPTYEVHASKKDVIRRLKALLKDADELYLATDEDREGEAISWHLLQVLKPKV
                     PVKRMVFHEITPSAIEHAVENWRDIDEGLVDAQETRRIVDRLFGYPVSEVLWRKVNRG
                     LSAGRVQSPAVRLVVERERERIAFRSADYWDIDAAFPTTPDFTGTLVEVDGTRVATGK
                     DFDDRGQLTRDAVVLDEPAARSLASALDASTFTVRSVEDKPFTSRPKPPFMTSTLQQE
                     GGRKLRMSSAQVMRVAQDLYQNGYITYMRTDSTTLSETALNAARNQVRSLYGDDYLPS
                     APRTYDRKVKNAQEAHEAIRPAGESFRTPAQVAGELRADALRLYELIWMRTVASQMVD
                     ARGFDKRVRIGATASDGRDTLFAASGRTITFPGYQRAYVEGTDDGGAAEDQQARLPDL
                     TEGLVLPTPALAPNGHTTSPPARYTEATLVKKLEELGIGRPSTY"
BASE COUNT         1028 a         2455 c         2559 g         1014 t
ORIGIN      
        1 gatcgttcgc catcgacgag tcgctccagc atgtcgtgaa cgacgggctg atggcgctgt
       61 tcttcttcgt ggtcggcctg gagatcaagc gggagctggt ggccggtgag ctgcgggacc
      121 gacgggtcgc ggtgctgcca gcggtggccg cgctgggcgg gatggtcgtg ccggccggca
      181 tctacgtcac cctcaacacc ggcggtcacg ggtcgcacgg gtggggcatc ccgatggcca
      241 ccgacatcgc cttcgccctc ggcgtggtgg cgctgctcgg ccgccgggtc ccggcgccgc
      301 tcaaggtgtt cctgctgacc ctcgccatcg tcgacgacat cggcgccatc gtcgtgatcg
      361 ccgtcttcta cagcgacggc atccggtgga cctggttcat cgaggcgctg gtggtcctcg
      421 gcctggtggt gctcgcccag cgggcgcggg tgcgctcgct cgtggtctac gtcgcgctcg
      481 gcgtggccgc gtggctgttc gtgttcgagt ccggcgtgca cgccaccatc gccggcgtcg
      541 tgctcgggct cctgacgccg gccaagtcgc tgctgccgga ggtggaggcc gaggcgatcg
      601 tcgaccagct cgagggccgc accgacctca ccgccgccga cgtgcaccgg gtcgagttcc
      661 tcgtccggga atcggtgccg cccaccgagc ggatcgagca cctcctccac ccgtggacga
      721 gctacgtgat cgtgccgctg ttcgccttcg ccaatgccgg catcgtgctc accggcgacg
      781 ccgtgacgag cccgtcttcg gtgctcgtcg gtgtcgtgct cggcctggtg ctcggcaaga
      841 ccgtcggcat cagcctgttc tcctggatcg ccgtcaagct cgggtggggc gtgctccccg
      901 atggcgtgcg gttcggccag ctggtcgggg tcgccgcgct cggcggcatc ggcttcaccg
      961 tgtcgctgtt catcaccgct ctggcgttcg agggtgagcc cctcctcgcc gaccaggcca
     1021 agatcggcat cctggtggcc tccctggccg ccgccgcgct cggctccgtc atcctcaccc
     1081 tcgccgcccg ccgctcgatt tcgggtcacc caggcccctg acgggtgcac aggcgacccg
     1141 aaatcatggg agcgcccgca ccccacatcg aatcgggtca cgcgtgcacc ggaggggtgc
     1201 agaggtgacc cgaaatcgcg tgagggtcag acgcgggcga cgtcgggggg gatctcggtc
     1261 tgggtgtagc ggccgagctc gcgcttggcg acctggcggc ggtggacctc gtcggggccg
     1321 tcggccaggc gcagggtgcg gatgccggcg tacatcgacg ccagcgggaa gtcgtcgctg
     1381 accccaccgc cgccgtgggc ctggatcgcc cggtcgatga cccgcagcgc catgttcggg
     1441 acgacgacct tgatggccga gatctcgatg gcggcaccct tgttgcccac cgtgtccatc
     1501 aggtacgccg ccttcagggt gaggaggcgg gcctgctcga tctccatacg ggagtcggcg
     1561 atccactcgc ggatcacgcc ttgctcgctc aggggccggc cgaacgccac gcggtcggtg
     1621 acccgctggc acatcagctc gagcgcccgc tccgccgcac cgatgcagcg catgcagtgg
     1681 tggatgcggc cggggccgag gcgggcctgg gccagggcga acccgctgcc ctcggtgccg
     1741 aggaggttgc tggccggcac ccgcacatcg gtgaactgga tctcgcagtg gccctcctgg
     1801 tcctggtacc cgaacaccgg cagggcccgc acgatctcga ggccaggtgc gtcgcggggc
     1861 acgaggatca tcgactgctg gacgtggcgg gccgcatcgg gatcggtctt ccccatgagg
     1921 atgaagagct ggcagcgctc gtcggcggcg cccgagatcc accacttgcg gccgttgatc
     1981 acgtactcgt cgccgtcgcg ctcgatgcga cacgagatgt tggtggcgtc ggagctggcc
     2041 accgccggct cggtcatggc gaaggcgctg cggatctggc ctccgagcag cgggtacagc
     2101 cactcctcct gctgctcggg cgtgccgaac atcgtcagca gctccatgtt cccggtgtcg
     2161 ggcgccgagc agttgcaggc ctgggaggcg atgtggctcc ggcccatgat ctccgccagc
     2221 ggcgcgtagt ccgagttcga caagccgtcg gtccactggg tcttgtgcgg gaggaagagg
     2281 ttccacaacc cccgcttgcg ggcctcctgc ttgagctcct ccatcaccgg cgggtgggtg
     2341 tgcgggtccc cggagtccac gatctgctgg tggtacacgg cctcggccgg gtacacgtgc
     2401 gagtccatga agtcgagcag ttcggtgcgg agcgcctcgg ccttggggga gtaggagaag
     2461 tccatgcgcc ggaccgtacc cgtcggtccc gagcgtcgcc tgagccgccc cgcagcgcgg
     2521 acgggaccga tcggcgagga gcgagggaga ggccgggaga catcggatgc cagacgagga
     2581 ccccgtgagc cgatcccgcg ctgggatcat ctcgccttcg ctcgtctcgt ggacatgacc
     2641 agatgatcgc ccgctgagtc gtcgccgtcg ccacgccgat gcctggcacg atccgcctgc
     2701 atcctcccag gcggcgtggc cgacccgccg gcttgcgccg ttcgtggctc gtgcggctcg
     2761 ggcggcgctc aggccgtgat gccgaagtcg atggggatga cggtgccgtt gatggcggtg
     2821 gcctcgtcgg agctgaggaa ggcgatgagg gccgcgggct cttcggcctg catcatccgg
     2881 tagcccgggg ccgccgtctt cttgacgagg tcgaagtcga tgccctcggg gaaggtcacg
     2941 gcgctggtca tgttcgtctc gacgccgccg ggggcgatgg cgttcgagcg gatgccgagc
     3001 ttctggtact cccaggcgat ggcccggctg agcatggcga cgccgccctt cgacgcggcg
     3061 tagccgggca tgtaggcctg gccgaacacg ccggcagacg acgcgaccgt ggtgatggcg
     3121 ccgccgccgt tggccagcat cgacgggatc acggcgcggg ccatgaggaa cgggcccttg
     3181 aggttgacgg cgatctggag gtcgaagtcc tcgacgctcg attcgtgggt gatgccgaag
     3241 cgcaggacgc cggcgacgtt ggccaggatg cgggtcggcc cgaggtcgcc ctcgatcgtg
     3301 gccaccgccg cggccacgct gtcgggatcg ctcacgtcca cggtgccggc gaccgcctgg
     3361 ccgccggccg cgaggatctc ggccgcggtc tcgtgcacgg actcggccat gtcgaggcag
     3421 ccgacggccg ccccgtcggc ggcgagccgc agggccgtgg ccttgccgat gccggacgcg
     3481 gcccccgtca cgagtgcggt gcggccgtcg aagcgggagg gagtcgtcat ggccgggacc
     3541 ctagaacgaa aatgagaacg cgttctcgtt tgggggcagg cccaggtgcc tcggtcctcg
     3601 tcgtcgaacg gctccggtga ggggcagccg acgacgagaa ccgacacggt tggtctgggg
     3661 tcggcgtggg acgatcggga gatggcagac gacgaccttc cggtgtctcg cggggtgcgc
     3721 atcccccggc gggagctggt ggtgtcgttc cgcgcgtccg gtgggccggg tggccagcac
     3781 gccaacaaga cggccacccg cgtcgagctc gtctacgacg tggagcactc gacggccctg
     3841 ctgcccgtgc agcgggagcg ggtgatgggc aagctcggcc cggtggtgcg gatcgtggtc
     3901 gacgacgagc gcagccagct ccggaaccgg gccatcgccg aggagcggct cgttgcccgc
     3961 ctcagccaag ccctccacgt ggaggcgccg aggcgcaaga ccaagccgtc gaagggcgcc
     4021 aaggagcgcc gcctccaggg caagaagcag cgctccgaga ccaaggcgaa ccggcgcccg
     4081 cctcgccacg acgcgtagcg cggcaagctc gagcatcggc tggccccacc acgaacctgg
     4141 tgtgaaagac gacgcaggag ggtggttgcc acaccaactt cggtggggtg ggcggtgcgg
     4201 cggggtcaga cagcgggagc ggcgggtgac gggacccggc tcgtccaagc caggcggctg
     4261 agcacgatga ccgcggcggc gaggagggcg aagaagtagc cgacgagcgc agcgtcgctg
     4321 accgcccacc cgtaggcgcc ccacgtgacg gcgtcgacca cggcgaccca ccaggtggcc
     4381 ggcgcgacgc ccgacaggtc gtgctcgcgc agcacccggc ggacctgcgg gccggcggtg
     4441 accagcaccg tgcccgcgag ggcgacaccg agcaccccga gcgccgcggc gacgaggagg
     4501 gcggcgacga accccgcgac cgcggccagg tcgcggcggg tcacctggtg ccggagcagt
     4561 actgcctgcc aggtgcaggg gaccagggcg aggaccgaca cgccccacac gaccggcagc
     4621 ccgtggacga gtccgtaggc gatccaggcc accgcggaga cgaccgcgtt gagggcggcg
     4681 gccgacgaga ccccggccgc cacgcccgac cgggcgaggc ggatgggttg gggcagcagg
     4741 cggaccagga acaccaccga tgcggcgacg gcgagggcat cggtgagcga cacggcgggc
     4801 gaagctagaa cgccgccgat atcagcttcc acgcaggtca gcggtcgttt gccacacgat
     4861 tccgggtcgg tgttcacact ccgggcccgc agcaccctgg cctcgctggg acctcgaccg
     4921 agcaggtggg gctcgtccgc ctggatcgga gcgggccggc cggtcccgcc ctaggtcaag
     4981 gcaggaggtc ggggcggtgt tcgcggatgg cgtcggcgag cttgccggcg ggccacttgg
     5041 gggcgaagtc gtcgatgggg atgatgcggt cccagccccg ctgctggacc tgggcccggg
     5101 tggcggcggt gtgggcccac cgcaggccgc ccttcttcag cacgaccgcc tgggtcttgc
     5161 ccgacggcag ggtggcctcg ccgatgcggt ccaggtcggc ccacgaggtg cgcagcacga
     5221 acgtgcccgc ctcgtactgg aggccgttgg cgtccacctt cagggagacg gtgcgctgct
     5281 gcccgacccc gaagaggagg cccacggtcc acacgaccac gaagatcagg gagacctgcc
     5341 agttgacgag cgccaggagg atcccggcga tcggaaggga ggcgacgaag atcatcacgc
     5401 cgaggcgcag gagccggtcg cgctgggacc gcgtggaggg cgtgaggacc gtcgacacgt
     5461 cgtctggcct agctcccgag aggtgtctcg gccaacctcg cggacccggg agcgcgagcc
     5521 cggatcgccg gtcggtgttg acagcggcct gtcgcatggc tttctctact gcgcgtgccc
     5581 aagccactgg tgatcgtcga gagcccggcc aaagcccgca ccatcgcggg cttcttgggc
     5641 aacgacttca acgtggagtc ttccgtcggg cacatccgcg acctgccgtc gagcggtctc
     5701 aacatcgacg tcgaccacca cttcgagccc acctacgagg tgcatgcgtc caagaaggac
     5761 gtcatccgcc ggttgaaggc gctgctgaag gacgccgacg agctctacct cgcgacggat
     5821 gaggatcgcg agggcgaggc catcagctgg cacctcctcc aggtgctgaa gccgaaggtc
     5881 ccggtgaagc ggatggtgtt ccacgagatc accccgtccg cgatcgagca cgcggtggag
     5941 aactggcggg acatcgacga gggcctcgtc gacgcccagg agacccggcg catcgtcgac
     6001 cggctcttcg gctacccggt gtccgaggtg ctgtggcgca aggtcaaccg gggcctgtcg
     6061 gccggccggg tgcagtcgcc ggccgtgcgc ctggtggtgg agcgggagcg ggagcgcatc
     6121 gcgttccggt ccgccgacta ctgggacatc gacgccgcct tccccaccac cccggacttc
     6181 accggcacgc tggtcgaggt cgacggcacc cgcgtcgcca ccggcaagga cttcgacgac
     6241 cgcggccagc tcacccgcga cgcggtcgtc ctcgacgaac cggccgcacg gtcgctcgcc
     6301 agcgccctcg acgcctcgac gttcaccgtc cgctcggtcg aggacaagcc cttcacgtcg
     6361 cggccgaagc cgccgttcat gacgtcgacc ctccagcagg agggtggccg caagctccgc
     6421 atgagctcgg cccaggtcat gcgcgtcgcc caggacctgt accagaacgg gtacatcacc
     6481 tacatgcgga ccgacagcac cacgctgtcg gagaccgccc tcaacgcggc ccgcaaccag
     6541 gtgcgctcgc tctacggcga cgactacctc ccgtcggcgc cgcgcaccta cgaccgcaag
     6601 gtcaagaacg cccaggaggc ccacgaggcc atccgcccgg cgggcgagtc gttccgcacc
     6661 ccggcgcagg tggccggcga gctgcgggcc gacgccctgc gcctctacga gctgatctgg
     6721 atgcgcaccg tcgcctcgca gatggttgac gctcggggct tcgacaagcg ggtccgcatc
     6781 ggcgccacgg cctccgacgg gcgcgacacc ctgttcgccg cctcgggtcg caccatcacc
     6841 ttccccgggt accagcgggc ctacgtcgag ggcaccgacg acggcggcgc cgccgaggac
     6901 cagcaggccc ggctgcccga cctcaccgag ggcctcgtcc tgcccacccc ggcgctggcg
     6961 cccaacggcc acaccacgtc gccgccggcc cgctacaccg aagccaccct cgtcaagaag
     7021 ctcgaggagc tcggcatcgg ccggccctcg acctac
//
LOCUS       DATMFS010000142         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_192093, whole genome shotgun sequence.
ACCESSION   DATMFS010000142 DATMFS010000000
VERSION     DATMFS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192093"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VNQ33_02630"
     CDS             complement(<1..380)
                     /locus_tag="VNQ33_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HWJ97025.1"
                     /translation="MNVVVCVKQIPDPADPGALNADHTLKRDGKLILDESDSYGVEMA
                     LQLVDKAGEGEVTLVSMAPNGEVSGLRTALAMGAAKAVLVSDDALKGAGALDTAKVLA
                     AAIGRVEGADLILAATESSDGYTGT"
     gene            complement(444..1241)
                     /locus_tag="VNQ33_02635"
     CDS             complement(444..1241)
                     /locus_tag="VNQ33_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97026.1"
                     /translation="MKTRLSPPARLLAAGAVALLVALGATACDPPRAPAKDRAIIFVH
                     GWSALGAGNDCNGNFGSLKTSLRNGGFTGAMVTIGYYDSDRNCDVNLRSWGSIDNGTS
                     WKELSKVFSKYVYETYTKKGVTVDLVGHSMGGLITRGAVQGAQAKEAGFSPPLKVEDS
                     VTFAAPFQGAAWFSTGCLWGQCSQLKPGASDIKWLLTNGAPQGVGGTDWTAFGSTADD
                     TVPSASALAINVPDARKVLYTNVEHSDYMGNAVAQARAAKALAEVDS"
     gene            complement(1242..2006)
                     /locus_tag="VNQ33_02640"
     CDS             complement(1242..2006)
                     /locus_tag="VNQ33_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97027.1"
                     /translation="MVLVAALLAVGATACEPARPDDKTKPVLLIHGWGLGADTDCGST
                     FDGMIAKLKAAGFTGPFVRVGFYSGGTNCDVNLRSWGPVEDGSHSKVVAQAFSKYVYA
                     TFTAKGKAVDVVGYSMGGNVARGAVQGAQAKEAGFSAPIDVEDVVLFGAPMNGAAWYS
                     SFCLWGMCSSLKPGSDDVTWLQKVGNPQGAKGTEWTAFGSDADAVTPVDSALKIDVPA
                     NRKVRYTDVPHTGSDNYMGRADVVARAAAALAKVDS"
BASE COUNT          313 a          787 c          781 g          354 t
ORIGIN      
        1 gtgccggtgt agccgtcgga cgactcggtg gcggcgagga tcagatcggc gccctcgacc
       61 cggccgatgg cggcggcgag gaccttggcc gtgtcgagcg caccggcgcc cttgagggcg
      121 tcgtcgctca cgaggacggc cttggcggcg cccatggcca gggcggtgcg gagaccggac
      181 acctcgccgt tcggggccat ggagacgagg gtgacctcgc cttcaccggc cttgtcgacc
      241 agctggagag ccatctcgac gccgtacgag tcggattcgt cgaggatgag ctttccatcg
      301 cgcttgaggg tgtgatccgc gttcagcgcg ccgggatcgg cgggatcggg gatctgcttg
      361 acacagacga cgacgttcat gccggggagt ctgttcggcg cgccggtccg cgacaaatcg
      421 acggcacgct ggtcgccggc cggctagctg tcgacctcgg cgagcgcctt cgcggcacgg
      481 gcctgcgcga cggcgttgcc catgtagtcg ctgtgctcga cgttcgtgta gagcaccttg
      541 cgagcatccg ggacgttgat cgcgagggcc gacgcgctgg gcacggtgtc gtcggccgtg
      601 gagccgaacg ccgtccagtc ggtgccgccc accccttgcg gtgcgccgtt ggtgagcagc
      661 cacttgatgt cgctcgctcc cggcttgagc tggctgcact ggccccagag gcagccggtg
      721 gagaaccagg cggcgccctg gaacggcgcc gcgaacgtga ccgagtcctc caccttgagc
      781 ggcggggaga accccgcctc cttcgcctgc gcgccctgga ccgcgccccg ggtgatgagc
      841 ccgcccatcg agtggcccac caggtccacc gtgacgccct tcttcgtgta ggtctcgtag
      901 acgtacttcg agaacacctt cgagagctcc ttccaggagg tcccgttgtc gatggagccc
      961 cagctgcgga ggttcacgtc gcagttgcgg tcgctgtcgt agtagccgat cgtgaccatc
     1021 gcgccggtga agccgccgtt gcgcagcgac gtcttgagcg atccgaagtt gccgttgcag
     1081 tcgttgccgg cgccgagggc cgaccagccg tgcacgaaga tgatcgcccg gtccttggcg
     1141 ggggcacggg gcggatcgca cgccgtcgcc cccagcgcca cgagcagggc caccgcaccg
     1201 gcggccagga gacgagccgg cgggctgaga cgggtcttca tctagctgtc caccttcgcg
     1261 agggccgccg cggcgcgggc gaccacgtcg gcgcggccca tgtagttgtc ggatccggtg
     1321 tggggtacgt ccgtgtagcg gaccttgcgg ttcgcgggca cgtcgatctt gagggcggag
     1381 tccaccgggg tcacggcgtc ggcgtcggag ccgaacgccg tccactccgt gcccttggcg
     1441 ccctgcgggt tgccgacctt ctggagccac gtcacgtcgt cggatcccgg cttcagggac
     1501 gagcacatgc cccagaggca gaagctcgag taccaggcgg caccgttcat cggcgcaccg
     1561 aacaagacga cgtcttcgac gtcgatcggc gccgagaagc cggcctcttt ggcctgggcg
     1621 ccctggacgg cgccgcgcgc cacgttgccg cccatcgagt agccgacgac gtcgaccgcc
     1681 ttccccttgg ccgtgaaggt cgcgtacacg tacttcgaga acgcctgggc gacgaccttc
     1741 gagtgcgagc cgtcctcgac cgggccccag ctgcgcaggt tcacatcgca gttcgtgccg
     1801 cccgagtaga agcccacccg cacgaacggg ccggtgaacc cggcggcctt gagcttggcg
     1861 atcatgccgt cgaaggtgct gccgcagtcg gtgtcggcgc ccaggcccca gccgtggatg
     1921 agcaggaccg gcttggtctt gtcgtcaggc cgggccggct cgcacgccgt cgcgccgacg
     1981 gcgagcaggg cggcgaccag gaccaccgct ccgagccggc gcgcgtggca ccgaccccgt
     2041 gcaggcacgt tcgaaccaac catcgcgatc tccccgattc cttctcgcgc tccccggagg
     2101 cagcggctcc ctggtcgcac gctacttcgg acatacgccc ggggaccacc cacccgccgc
     2161 gacctcgccc ggtggccggc cgagcgccta ccgtcgcccc tgcgtgaagg tcctgctgct
     2221 cgtcaacccg ttctc
//
LOCUS       DATMFS010000143         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_284859, whole genome shotgun sequence.
ACCESSION   DATMFS010000143 DATMFS010000000
VERSION     DATMFS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284859"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VNQ33_02645"
     CDS             <1..717
                     /locus_tag="VNQ33_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWJ97028.1"
                     /translation="DAVVFLSEFQPAVDQAVGEAQGEVFDARDATELLPAEETEGDTR
                     VEADEHEHEGDGHGHGHDEVGTTDPHFWLDPTKMAEVATAFAATLARADPAHTADYRA
                     NAEALVGRLDDLDAEMEAGLADCEQSGLVTSHEAFGYLARRYGFEQVGITGLTPEADP
                     SPKDLATVADDASERGVTTIYFETLTSPAVAETLAAEAGAKTAVLDPIEGLTDESNGR
                     DYLEVMRSNLETLRTGQDCA"
     gene            714..1499
                     /locus_tag="VNQ33_02650"
     CDS             714..1499
                     /locus_tag="VNQ33_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWJ97029.1"
                     /translation="MSGEQPADAPVVEFRGCDIGYERRAVVRGLDLSIASGEVLGILG
                     ANGSGKTTTIRGLLGLTPILAGEVRLFGTPRGQFHDWARIGYVPQRHTVAGGIPSTVG
                     EVVASGRLTRLRPWRRSTAEDRTAVADAIAVVGLAGQERTTMTALSGGQQRRVLIARA
                     LATRPDLLVLDEPTAGVDRANQEALAVALGALVDQGTTIVLITHELGPVEPLITRAVV
                     LRDGVVVHDGPPLQDEHHHHHDDDWHHTHGDEPVRRPDLGLLG"
     gene            1510..2343
                     /locus_tag="VNQ33_02655"
     CDS             1510..2343
                     /locus_tag="VNQ33_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWJ97030.1"
                     /translation="MTILHYEFMQRALLASVLIGLSAPSVGVYLVQRRLSLIGDGLGH
                     VALAGVAVGVLTSTSPIWAALVAAVAGAVAIELIRSRGRTSGDVALAVLFYGGIAGGV
                     VLISQAPGGTPSNLNAYLFGAITTTTPSDLVVFAVLSAIVLLVTHGLGRYLFAVSNDE
                     EYATASGLPVLPLNLLLAVMTAVTVVVSMRVVGLLLISALMIVPIATAQLIAGSFRAT
                     VAIGTVIGVAVSVAGVTTSYYQDTPSGGTIVLFAIALFVLAAAIAGVRDHRRQRAAQP
                     V"
BASE COUNT          324 a          957 c          821 g          339 t
ORIGIN      
        1 gacgccgtcg tcttcctgtc cgagttccag ccggccgtcg accaggcggt gggggaagcg
       61 cagggcgagg tgttcgacgc ccgtgacgcc accgagctgc tgcccgccga ggagacggag
      121 ggcgacaccc gtgtcgaagc cgacgagcac gagcacgaag gcgacggcca cggccacggc
      181 cacgacgagg tggggaccac cgatccgcac ttctggctcg accccacgaa gatggccgag
      241 gtcgccaccg ccttcgccgc caccctcgcc cgggccgacc ctgcccacac cgcggactac
      301 cgggcgaacg ccgaggcgct cgtcgggcgg ctcgacgacc tggatgccga gatggaggcg
      361 ggcctcgccg actgcgagca gagcggcctc gtcaccagcc acgaggcgtt cggctacctg
      421 gcccggcgct acggcttcga acaggtcggc atcaccggcc tgacccccga ggccgatccc
      481 tcgccgaagg acctggccac cgtcgccgac gacgcctccg agcgcggcgt cacgacgatc
      541 tacttcgaga cgctcaccag ccccgccgtc gccgagacgc tcgccgccga ggccggcgcg
      601 aagacggcgg tgctcgaccc gatcgagggc ctgaccgacg agtccaacgg ccgcgactac
      661 ctcgaggtca tgcgctcgaa cctcgaaacg ctccgaaccg gtcaggactg cgcgtgagcg
      721 gcgagcagcc cgccgacgcg cccgtcgtcg agttccgggg ctgcgacatc ggctacgaga
      781 ggcgggccgt cgtgcgcggg ctcgacctct cgatcgcctc gggcgaggtg ctcggcatcc
      841 tcggggccaa cgggtccggc aagaccacca ccatccgggg gctcctgggc ctgacgccga
      901 tcctcgccgg cgaggtccgg ctgttcggca ccccgcgggg ccagttccac gactgggccc
      961 gcatcgggta cgtgccccag cgtcacacag tggccggcgg gatcccgtcg acggtcggcg
     1021 aggtcgtggc gtcgggacgg ctcacccgcc tgcgcccctg gcgccgctcc accgccgagg
     1081 accgcaccgc agtggccgat gccatcgccg tcgtcggcct ggccggccag gagcgcacca
     1141 ccatgacggc cctctcgggc gggcagcagc gccgtgtcct catcgcccgg gcgctggcca
     1201 cccggccgga cctcctcgtg ctcgacgagc cgacggccgg cgtcgaccgc gccaaccagg
     1261 aagccctcgc cgtcgccctc ggtgccctcg tcgaccaggg caccacgatc gtgctcatca
     1321 cccacgagct gggtccggtg gagccgctca tcacccgggc ggtcgtgctg cgcgacggcg
     1381 tggtggtcca cgacggcccg ccgctccaag acgagcacca ccaccatcac gacgacgact
     1441 ggcaccacac ccacggcgac gagcccgtcc gccgtccgga cctcgggctg ctgggctgag
     1501 gcgggcggca tgacgatcct ccactacgag ttcatgcagc gggcgctgct cgcctccgtc
     1561 ctcatcgggc tgtcggcgcc gtcggtcggc gtgtacctgg tgcagcggag gctgtcgctc
     1621 atcggcgacg gcctcgggca cgtcgccctc gccggcgtcg ccgtcggcgt gctcacctcg
     1681 acctcgccca tctgggcggc gctcgtcgcc gccgtcgccg gcgccgtggc catcgagctc
     1741 atccgcagcc ggggccggac cagcggcgat gtggccctcg ccgtgctctt ctacggcggc
     1801 atcgccggcg gcgtcgtcct catctcgcag gcacccggcg gcacgccgag caacctgaac
     1861 gcctacctgt tcggcgccat cacgaccacc acgccgtctg acctcgtcgt gttcgccgtg
     1921 ctgtcggcca tcgtgctgct cgtcacccac ggcctcggcc gctacctgtt cgccgtcagc
     1981 aacgacgagg agtacgccac ggcgtcaggc cttcccgtgc tgcccctgaa cctgctcctc
     2041 gccgtgatga cggccgtcac cgtcgtggtg tcgatgcggg tcgtcggcct gctgctcatc
     2101 agcgcgctga tgatcgtgcc catcgcgacg gcccagctga tcgccgggag cttccgggcc
     2161 accgtcgcca tcggcaccgt gatcggcgtc gccgtgtccg tggccggggt gacgacgtcg
     2221 tactaccagg acaccccgtc gggcgggacg atcgtgctgt tcgccatcgc cctgttcgtg
     2281 ctggccgccg ccatcgccgg cgtccgcgac caccgccgcc agcgagcggc ccagcccgtc
     2341 tgaccgttgc ggcgggcacg acacgggtcc catcccagcg atcctcccga tcggtgccgt
     2401 gctgccgatg ggtaccggtg tctgagcccc ttgccgttcg g
//
LOCUS       DATMFS010000144         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_51730, whole genome shotgun sequence.
ACCESSION   DATMFS010000144 DATMFS010000000
VERSION     DATMFS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51730"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..828)
                     /locus_tag="VNQ33_02660"
     CDS             complement(142..828)
                     /locus_tag="VNQ33_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97031.1"
                     /translation="MDHPTTHEPTLDDQRTDQLRTFLTVEAAAGAAAAPTRHPGRRIL
                     AGGAIALFLGGVLFAATSVGGGPSSPVATRAEAAVSITPAGDGWIDVRLADIDADPDQ
                     VVRELRAAGIDASKEPIASVSLSPSGEPMLSSHGPDGSATIDAKPSDLQVFGLVDGKG
                     GPGGHGLAGISVKVPEGIALPPDGPEAPIDPSSPFNQAMERHGVRLAEDGLVSVRRGA
                     AVTIVVLVAA"
     gene            complement(831..1451)
                     /locus_tag="VNQ33_02665"
     CDS             complement(831..1451)
                     /locus_tag="VNQ33_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ97032.1"
                     /translation="MTEADDVELWSRIRADDADAFGLLFQRHGARIHGYVLRRTGDPQ
                     AAEDIVALVFLEAWRRRGDVDLNQPSALPWLYGVAANVLRHQHRSRRRHQAALDRLAH
                     LPARSPALVERQAEAAADAQRVLEQVQALPRRERDVLVLSVWEGLSHAQIAVALDTTI
                     GTVKSRLSRARARLDPDRRPSAPSEPPSPAPTAAPPAPTVLTLKES"
     gene            1626..>2069
                     /locus_tag="VNQ33_02670"
     CDS             1626..>2069
                     /locus_tag="VNQ33_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97033.1"
                     /translation="MNRVRPADEEQVADVVSDAPSAPGSPVPAATQVVTAARWTRPQK
                     RAAEVLTPDARCSPVLLRAAIVGKARMPRAALEVLAPNGAVIGHVALPNTSESFDLRR
                     RYWLVGDPAPGSVVAFVSEHRTQVILPGSRLKEGPAVAQTWSGVAC"
BASE COUNT          252 a          729 c          810 g          278 t
ORIGIN      
        1 ctcgctcacc cggttgcgga tcggtgcggg acggtcagca tcggccatgg ccgcggaccc
       61 tacgcgaagg tggcgcacac ccgaccgccg ctcgaccggg tgtgcgccac agggtggccg
      121 gcgaaggccc gccggcgtgg atcaggcggc cacgagcacc acgatcgtga ccgcggcgcc
      181 ccggcgcacc gacacgagcc cgtcctcggc caggcggacg ccgtggcgtt ccatcgcctg
      241 gttgaacggg gacgaggggt cgatgggcgc ctcggggccg tcgggtggga gagcgatccc
      301 ttcgggcacc ttcaccgaga tgccggccag cccgtgccca cccggacccc ccttcccgtc
      361 gaccaagccg aagacctgga ggtcggacgg cttcgcgtcg atcgtggcgg atccatcggg
      421 tccgtggctg ctgagcatcg gctcgccgga cggggacagc gagacggacg cgatcggctc
      481 cttgctggcg tcgatgcccg cggcgcgcag ctcgcgcacc acctggtccg ggtcggcgtc
      541 gatgtcggcg aggcgaacgt cgatccagcc gtcccccgca ggcgtgatcg acaccgcggc
      601 ctcggcccgg gtggcgacgg gagaggaggg cccgccgccg accgaggtcg cggcgaacag
      661 cacgccgccg aggaacaggg cgatcgcgcc gcctgcgagg atccgccggc cgggatggcg
      721 ggtcggtgcc gccgcggccc ccgcggccgc ttcgacggtg aggaacgtgc ggagctggtc
      781 ggtccgctgg tcgtccaggg tgggttcgtg ggtggtcggg tggtccatgg tcaggactcc
      841 ttcaaggtga gcacggtggg tgctggtggt gcggcggtcg gtgcgggcga aggcggctcg
      901 gacggcgcgc tgggccgccg gtcggggtcg agccgggccc gggcccggga gaggcgggac
      961 ttgaccgtcc cgatggtggt gtcgagggcg acggcgatct gggcgtgcga gaggccctcc
     1021 cacaccgaca gcacgagcac gtcgcgctcg cgacggggca gggcctggac ctgctcgagc
     1081 acccgctggg cgtcggcggc cgcctctgcc tggcgttcca cgagcgcggg cgatcgggcc
     1141 gggaggtgag cgagccggtc gagtgcggcc tggtggcgcc ggcggctgcg gtgctggtgg
     1201 cgcaggacgt tcgccgcgac cccgtagagc cagggcagcg ccgatggctg gttcagatcg
     1261 acgtcgcctc gccgtcgcca cgcctcgagg aacacgaggg cgacgatgtc ctcggcggcc
     1321 tgggggtcac cggtgcgtcg cagcacgtag ccgtggatgc gggccccgtg gcgctggaag
     1381 aggaggccga acgcgtcggc gtcgtcggcg cggatgcggc tccacagctc cacgtcgtcc
     1441 gcttcggtca tcggtggctc ctcgccgctc aggtggttga cacccctcca tgcccgcacc
     1501 gccccgaagg ttccatcttt cccgaggaac ccgaggaacc cggcggccac cgccctcgcc
     1561 cgtccccgcc gtcgtccgac cgccaggccg tgctgtcggc ggcaagaacg gtggtgcgct
     1621 cgctcatgaa ccgggtgcga ccggccgacg aggagcaggt ggccgatgtc gtcagcgatg
     1681 ctccgagcgc gcccgggtct ccggtccctg ccgcgacgca ggtcgtgacg gcggcccggt
     1741 ggacgcggcc gcagaagcgg gcggccgagg tcctcacccc cgacgctcgg tgctcgccgg
     1801 tgctgctgcg agctgccatc gtcggcaagg cccggatgcc gcgtgcggcc ctggaggtcc
     1861 tcgctccgaa cggggcggtc atcggccacg tcgccctgcc caacacgtcc gagtcgttcg
     1921 acctgcgccg gcgctactgg ctggtgggcg acccggcgcc ggggtcggtc gtggcgttcg
     1981 tgtccgagca ccggacccag gtgatcctcc ccggcagccg gctgaaggag ggcccggctg
     2041 tcgcccagac gtggtccggc gtcgcctgc
//
LOCUS       DATMFS010000145         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_215088, whole genome shotgun sequence.
ACCESSION   DATMFS010000145 DATMFS010000000
VERSION     DATMFS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215088"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..626)
                     /locus_tag="VNQ33_02675"
     CDS             complement(45..626)
                     /locus_tag="VNQ33_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ97034.1"
                     /translation="MPEDPRDRILAATVACLGRYGIAKTTVDDAAREAGLARATVYRY
                     FPDGKEQLIAESITWAVAQFFHELAVAVMDAPDFPTLVEQALMHAHRAVAEHVVLQKV
                     LETEPERLLPQLTQSAPQVQAVLRDYLADKLRDEDLLAGIDPLEAGDWLARMGLSFIL
                     AEGRWDLTDPASVRRLVREELLASILPPASLDA"
     gene            688..1149
                     /locus_tag="VNQ33_02680"
     CDS             688..1149
                     /locus_tag="VNQ33_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ97035.1"
                     /translation="MSTVLLATDGSDLATAAMARGVDLLGHEHRFITLAVVLPVFVPT
                     ASVSPMDTHPTLVDAELEEELEAEQRAAQDDGVHELDEVLGITAQHLVETGEPGPTIC
                     DVATEVGAEIIVIGSHGHGLLKRVLMGSVSTHVLHHAPCPVLVIRTDQPER"
     gene            complement(1208..2089)
                     /locus_tag="VNQ33_02685"
     CDS             complement(1208..2089)
                     /locus_tag="VNQ33_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWJ97036.1"
                     /translation="MSRYRFALKPKWILSHIFVLVMILGMVRAGIWQWDRLQQRKEIN
                     ARVEAVQQKAPEPVAGLAEPGDFGKGRDLEYRPVTATGTYLADQSVLVDGADHEGAAG
                     AWVLTPLKLDDGDVVIIKRGTIANPGQLDAVPESVETPTGEVTVEGLARPTETRPEVN
                     LGFTKLHFERADPPTGTLENMARTDLERLDQQIDGDVLPFSISLRTQDPEVTADEPLP
                     PNAPDLSEGSHFSYTVQWFTFSSMTIVVYALILRRRAREIEREALEAELDQADPDDEP
                     EDGDPRVAPDAPSGSTA"
BASE COUNT          307 a          811 c          827 g          304 t
ORIGIN      
        1 ccccgacggc gcgccggccg cgggcccgac gggtgcgatc ggggtcacgc gtcgagcgaa
       61 gcgggcggca agatcgaggc gagcagctcc tcgcggacga gccgccggac cgacgccggg
      121 tcggtcaggt cccaccggcc ctcggcgagg atgaacgaca gacccatccg cgccagccag
      181 tcccccgcct ccagcgggtc gatccccgcc agcaggtcct cgtcgcgcag cttgtcggcc
      241 aggtagtccc gcagcacggc ctgcacctgc ggcgccgact gggtcagctg cggcaggagc
      301 cgctccggct ccgtctcgag caccttctgg agcacgacgt gctccgcgac cgcccggtgc
      361 gcgtgcatga gcgcctgctc cacgagcgtc gggaagtccg gggcgtccat caccgccacg
      421 gcgagctcgt ggaagaactg ggcgacggcc caggtgatcg actcggcgat cagctgctcc
      481 ttgccgtccg ggaagtagcg gtagacggtg gcccgggcga ggccggcctc gcgcgccgcg
      541 tcgtcgacgg tggtcttggc gatgccgtag cggccgaggc acgcgacggt ggccgccagg
      601 atgcggtcgc gggggtcctc cggcacgccg cccacgctac ccacgccccg gaacgggacg
      661 gggtgggcgg cgcgcgagca tggcggcatg tccaccgtgc tgctcgccac cgacggatcc
      721 gacctcgcca ccgccgccat ggcccgaggc gtggacctgc tcggccacga gcaccgcttc
      781 atcaccctcg ccgtcgtgct gccggtgttc gtgccgacgg cctcggtcag ccccatggac
      841 acccatccga ccctggtcga tgccgagctc gaggaggagc tggaggccga gcagcgggcc
      901 gcccaagacg acggggtcca cgagctcgac gaggtgctcg gcatcaccgc ccagcacctg
      961 gtggagacgg gcgagcccgg cccgaccatc tgcgacgtgg ccaccgaggt cggcgccgag
     1021 atcatcgtga tcggcagcca cggccacgga ctgctcaagc gggtgctgat ggggtccgtg
     1081 agcacccacg tgctgcacca cgccccctgc ccggtcctgg tgatccggac cgaccagccc
     1141 gagcgctgac gctgctcccg gatcgcctgc gcacctccgc ggtgcggaga cgacccgaag
     1201 gcggcggtca ggcggtggag ccgctcggcg cgtcgggcgc gacccggggg tcgccgtcct
     1261 caggctcgtc gtcgggatcg gcttggtcga gctcggcctc gagcgcctcc cgctcgatct
     1321 cccgtgcccg gcggcgcagg atgagcgcgt acacgacgat ggtcatggac gagaacgtga
     1381 accactgcac ggtgtacgag aagtgggagc cctcggacag gtcgggggcg ttcgggggca
     1441 gcggctcgtc ggcggtcacc tccgggtcct gcgtgcggag gctgatggag aagggcagca
     1501 cgtcgccgtc gatctgctgg tcgaggcgct ccaggtccgt ccgggccatg ttctcgaggg
     1561 tgcccgtcgg cgggtcggcc cgctcgaagt ggagcttggt gaagcccagg ttgacctcgg
     1621 gccgggtctc ggtgggtcgg gcgagcccct cgacggtgac ctcgcccgtg ggcgtctcga
     1681 ccgactcggg gacggcgtcg agctggccgg ggttcgcgat ggtcccccgc ttgatgatca
     1741 cgacgtcgcc gtcatcgagc ttcaggggcg tgaggaccca ggcgcccgcg gcgccctcgt
     1801 ggtcggcgcc gtcgaccagc acgctctggt cggcgaggta ggtaccggtg gcggtgaccg
     1861 ggcggtactc caggtcccgg cccttgccga agtcgcccgg ttcggccagc cccgcgaccg
     1921 gttcgggggc cttctgctga acggcctcga cgcgggcgtt gatctccttg cgctgctgca
     1981 gacggtccca ctgccagatg ccggcgcgga ccatgcccag gatcatcacc agcacgaaga
     2041 tgtgcgagag gatccacttg ggcttgaggg cgaagcggta gcgcgacatg ccccggcgag
     2101 gctacgaccg gacccctcct ccgccgaagt cggcggcacc tcgacatccc ggagcgagcc
     2161 aacggaccgg cccggccagc cgctcccggt ccgaaggtgt cgaacgacct cggcccggtc
     2221 ccgcgaccgg ctccccggcg gccgcggaa
//
LOCUS       DATMFS010000146         3985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_122411, whole genome shotgun sequence.
ACCESSION   DATMFS010000146 DATMFS010000000
VERSION     DATMFS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122411"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="VNQ33_02690"
     CDS             complement(<1..1883)
                     /locus_tag="VNQ33_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ97037.1"
                     /translation="MRGSRHPGVWAIKRRSSTKSGPTGAPERRAATELAVLACACAVI
                     AGLAMFTRVFDRVIDWTSDSTNLNLNGVFALLIMVPVATTIFARRRYKDAISVRKELV
                     RLSLHDALTGLPNRMLLADWLAADIQTSQRRNSQAAVLFVDLDKFKQVNDTHGHEVGD
                     RLMRAVADRLRTMLRPEDRVIRFGGDEFVVLCPDVNSAAAGEKIANRVIEIIEQPFTI
                     GDEHLRISASVGVALAEHRGVKPEDVLRDADVAMYQAKAQGSGHVTVFDRSMTGTLTP
                     ATAEEQIRDALEAGEFHLHYQPVVEMATGRIVGAEALLRWISPDRGTMSPAEFIPILE
                     ETGLIVPVGTWVLEEACREAARVAAICDGQKPPTITINVSARQLAQLDFGDKVASALQ
                     SAGVAAGQIHLEITEGALMHDVTSAWAVLRQAKALGVKLALDDFGTGYSSLSYVRRFS
                     LDMLKIDKSFIDGIDSSPEDRAIVEHVVGMAQALGMVTVAEGVERPEQLQWLRRLGCR
                     LAQGYVMSRPVPGEEFEAILERHEIDPFHITAGEVPEIIAPVDEHERPDFIPAPGPAK
                     PAAVAVVDRPTPARSMVGPLEFAAPAPEPADDEAPLVAAAVGADKTGDQVAADRLGAP
                     TLPR"
     gene            complement(1975..>3985)
                     /locus_tag="VNQ33_02695"
     CDS             complement(1975..>3985)
                     /locus_tag="VNQ33_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97038.1"
                     /translation="GGEPFTVDLGEASRSTDPKSEGQRVDFPKQSFSTMDIEITETDP
                     GELRRYDGISPVGFAEVGVIDESGDRPLADDVIRLPKDLLDTAGKASIDNRLAIVLTR
                     QRVAGTVAVRSDPEPSMARTFSLPAARDFQLVGQVRLANAAADPVVDEALGLPDADHG
                     GVTATSNRRMPGGLSNRAMNAIDGDPSTWYSPGFLAQQQEYVSYDVADPITFGSMNLT
                     VLNDGRHSVPRKLYLDVDGKRVQTITLPDIGDQAEPNAQYTFPITLDKSVTGTKITLS
                     VAESDDAVREVKTLDWFTDRPLVMPMGIAELGIDGLKAPKTPERIDDRCRADLVTIDG
                     KAVPVSLHGTSADLLAGKPIDLVTCNDEAVGLPAGSTLLRTAEGRSSGFDLDRLVLRS
                     AAGGEADLGGGPLVDPRTQTPRPEMATTHETRTSVDVRVPKADEPFWLVLGQSDNLGW
                     HATADGKDLGPPTLVNGYANGWKVPAGSDIAIHAEWTPQKVVWSMIYASLAFVALALA
                     LVFWPRRRPAAADELDEPGWIPLDQRPSMPLPLRWDRIRRYTGPAPSLFAWIGTTLGA
                     LAFGFAFTGPVGAVILTAAAAVTLRVARSRPLLTIGAPALLVLTIGWMAGRQLFQELP
                     PGFDWPTYFEATQSPAWIAVLALLLDAVVERCWLRRWWPTDGTDA"
BASE COUNT          546 a         1286 c         1569 g          584 t
ORIGIN      
        1 cgggggagcg tgggggcgcc cagccggtcg gcggcgacct ggtcgcccgt cttgtcggct
       61 ccgacggcgg cggccacgag cggggcctcg tcgtcagccg gttcgggggc gggggccgcg
      121 aactcgagcg gacccaccat cgagcgggcc ggggtcggcc ggtcgacgac ggcgacagcg
      181 gcgggcttgg ccgggccggg tgccgggatg aagtcggggc gctcgtgctc gtcgaccgga
      241 gcgatgatct cgggcacctc gccggcggtg atgtggaacg ggtcgatctc gtggcgctcg
      301 aggatggcct cgaactcctc gcccggtacg gggcgggaca tcacgtagcc ctgggcgagg
      361 cggcagccga gccggcggag ccactgcagc tgctcgggcc gctcgacgcc ctcggccacc
      421 gtgaccatgc cgagcgcctg ggccatgccg acgacgtgct cgacgatggc ccggtcctcg
      481 gggctcgagt cgatgccgtc gatgaagctc ttgtcgatct tgagcatgtc gaggctgaac
      541 cggcggacgt aggagagcga cgagtagccc gtcccgaagt cgtcgagcgc cagcttgacg
      601 cccagcgcct tggcctggcg caggacggcc caggccgacg tcacgtcgtg catgagggcg
      661 ccctcggtga tctcgaggtg gatctggccg gcggcgacac cggcgctctg gagggcgctg
      721 gcgaccttgt cgccgaagtc gagctgggcg agctgccggg ccgacacgtt gatggtgatg
      781 gtggggggct tctggccgtc gcagatggcg gcgacgcgcg ccgcctcccg gcacgcctcc
      841 tcgagcaccc aggtgccgac ggggacgatg aggccggtct cctcgaggat cgggatgaac
      901 tcggcgggcg acatggtgcc ccggtcgggc gagatccagc gcagcagggc ctcggcgccg
      961 acgatccggc cggtggccat ctcgacgacc ggctggtagt gcaggtggaa ctcgcccgcc
     1021 tcgagggcgt cgcggatctg ctcctccgcc gtggcggggg tgagcgtgcc cgtcatcgac
     1081 cggtcgaaga ccgtgacgtg gcctgacccc tgggccttgg cctggtacat ggccacatcg
     1141 gcgtcgcgca ggacgtcttc gggcttgacg ccacggtgct cggcgagggc gacgccgacg
     1201 ctcgccgaga tgcgcaggtg ctcgtcgccg atggtgaacg gctgctcgat gatctcgatg
     1261 acccggttgg cgatcttctc gcctgccgcc gcgctgttga cgtcgggaca gaggacgacg
     1321 aactcgtcgc cgccgaagcg gatgacccgg tcctcgggcc gcagcatggt ccggaggcgg
     1381 tcggccacgg cgcgcatcag ccggtcaccg acctcgtggc cgtgggtgtc gttgacctgc
     1441 ttgaacttgt ccaggtccac gaagagcacg gcggcctggc tgttgcggcg ctgcgaggtc
     1501 tggatgtcgg cggcgagcca gtcggccagg agcatgcggt tgggcagccc ggtcagggca
     1561 tcgtgcagcg acaggcgcac cagctccttg cggacgctga tggcgtcctt gtagcgccgc
     1621 cgggcgaaga tcgtcgtggc caccgggacc atgatgagca gggcgaacac gccgttgagg
     1681 ttcaggttcg tcgagtcgct ggtccagtcg atcacccggt cgaacacccg ggtgaacatg
     1741 gccagcccgg cgatgaccgc gcacgcgcac gcgaggacgg cgagctcggt ggcggcccgg
     1801 cgctcggggg cgccggtcgg gcctgacttg gtgctggatc gacgcttgat ggcccatacc
     1861 cccggatgac gagaccctct caccggtctc cgactcgtgc ggtcctatcg gcagggggtc
     1921 cacgaggttg aggcgtcggt catcagacga cctccaggcc cggaggtgcc ctggtcaggc
     1981 gtcggtgccg tcggtgggcc accaccggcg gagccagcac cgctcgacga cggcgtcgag
     2041 caggagcgcc aggacggcga tccaggcggg ggactgggtg gcctcgaagt aggtcggcca
     2101 gtcgaagccg ggtggcagct cctggaacag ctgacggccg gccatccacc cgatcgtgag
     2161 gacgaggagg gcgggagcgc cgatcgtgag cagcggccgg gaccgggcga cccggagggt
     2221 cacggcggcg gcggcggtga ggatgaccgc gccgacgggc cccgtgaagg cgaacccgaa
     2281 cgccagcgcg cccagggtcg tgccgatcca ggcgaacagg gagggagccg gaccggtgta
     2341 gcggcggatc cggtcccaac gcagcgggag cggcatgctc ggccgctggt cgagcgggat
     2401 ccaccccggc tcgtcgagct cgtcggcagc cgcggggcgc cggcggggcc agaagaccag
     2461 cgccagggcc aaggccacga aggcgagcga ggcgtagatc atcgaccaga cgaccttctg
     2521 gggcgtccat tcggcgtgga tggcgatgtc ggagccggcg ggcaccttcc agccgttcgc
     2581 gtagccgttg acgagcgtgg gcgggccgag gtccttcccg tcggcggtgg cgtgccaacc
     2641 caggttgtcg ctctggccga ggacgagcca gaagggctcg tcggccttgg ggacgcggac
     2701 gtcgacgctc gtgcgcgtct cgtgcgtggt ggccatctcc ggccgagggg tctgggtgcg
     2761 ggggtcgacc agcggtccgc cgccgaggtc ggcctcgcca ccggccgcgg agcggagcac
     2821 cagccgatcg aggtcgaagc ccgacgagcg gccctcggcc gtgcgcagca gggtgctgcc
     2881 agcggggagg ccgacggctt cgtcgttgca ggtcaccagg tcgatcggct tgccggcgag
     2941 gaggtcggcg gaggtgccgt ggagggagac gggcaccgcc ttgccgtcga tggtcacgag
     3001 gtcggcgcgg cagcggtcgt cgatgcgttc gggcgtcttg ggggccttca gcccgtcgat
     3061 gcccagctcg gcgatgccca tgggcatgac caacggccgg tcggtgaacc agtcgagcgt
     3121 cttgacctcg cggacggcgt catcggactc cgccaccgac agcgtgatct tcgtgccggt
     3181 cacggacttg tcgagcgtga tcggaaacgt gtactgggcg ttcggctcgg cctggtcgcc
     3241 gatgtccggg agggtgatgg tctggacccg cttgccgtcg acgtcgaggt acagcttccg
     3301 gggcacggag tggcggccgt cgttgaggac ggtcaggttc atcgagccga aggtgatcgg
     3361 gtcggcgacg tcgtagctga cgtactcctg ctgctgggcg aggaagccgg gggagtacca
     3421 ggtgctgggg tcgccgtcga tggcgttcat cgcccggttg gacaggccgc ccggcatgcg
     3481 ccggttggag gtggcggtca cgccgccgtg gtcggcgtcg gggagcccga gcgcctcgtc
     3541 gaccaccgga tcggcggcgg cgttggccag gcgcacctgc ccgaccagct ggaagtcgcg
     3601 agctgctggc agcgagaagg tgcgggccat cgacggctcc gggtccgagc ggacggccac
     3661 ggtcccggcg acgcgctggc gggtcaagac gatcgcgagc cggttgtcga tcgacgcctt
     3721 gccggcggtg tcgaggaggt ccttcggcag gcggatcacg tcgtcggcga gcgggcggtc
     3781 gccggactcg tcgatgaccc ccacctcggc gaacccgacc gggctgatcc cgtcgtagcg
     3841 gcgcagctca ccggggtcgg tctcggtgat ctcgatgtcc atggtcgaaa acgactgctt
     3901 cgggaagtcg acccgctggc cctcgctctt cgggtcggtg ctgcgcgacg cctcgccgag
     3961 gtccaccgtg aagggctcgc cgccg
//
LOCUS       DATMFS010000147         5613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_308118, whole genome shotgun sequence.
ACCESSION   DATMFS010000147 DATMFS010000000
VERSION     DATMFS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308118"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1092
                     /locus_tag="VNQ33_02700"
     CDS             127..1092
                     /locus_tag="VNQ33_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWJ97039.1"
                     /translation="MALSKVWVHAELTPEGNVAPISLEILAKAREIADTVEVFTGSDG
                     SAVAATAGAHGATKVLATGDLSGKLQGVHVAAALAAAIEGGDVPDLILAGTTYDGRDI
                     AARLSVKLDKPVLTNNIDVDVTDGIVVTTPIFGGTQLVKTKFAAGAPAIALFRPKSFV
                     AEESGGGAAEVATIAVPDTGAAGAAKLLNRHVEETTGPKLDEAAVVVAGGRGLGEAAK
                     FELIEDLAKTLKGAPGASRAIVDAGWVPYSYQVGQTGKVVKPTVYIAAGISGATQHMV
                     GMKGSKNIIAINKDPEAPIFGVADLGIVGDVHKVLPKLLEALQAR"
     gene            1165..1587
                     /locus_tag="VNQ33_02705"
     CDS             1165..1587
                     /locus_tag="VNQ33_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97040.1"
                     /translation="MSLRDRLRNRRNDGALRAKLSQGGPVRVPCRLRRTSARGWGPWT
                     EGRVDLGAIPDGEARWSTDDAVAVGFASTNPRVDLPFEEVSDVYLRAVRFRTEAFWGM
                     ENDIVVVVSERGTIEVAVTPGDAEAVAARLAELLQPAL"
     gene            1639..2310
                     /locus_tag="VNQ33_02710"
     CDS             1639..2310
                     /locus_tag="VNQ33_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWJ97041.1"
                     /translation="MTGEDLTIDQLAAEVDMTVRNIRAHQARGLLPPPTIKGRIGYYG
                     PLHRRRLQQIRAMQDEGLNLAAIAKVVNGGQLTAVTTEAFTVSEPAWFEPQELLTRLG
                     DPDDAVTARAIDLGLIVLDGDRVRMDMPGLLPLAEEFLALGVPLAAQLDALAGVQAAT
                     EQVAAAYLQLADEHLIARVAVDSGGELEAIRASVARLGDLARVALDASFNHAMTEALR
                     TYFEQ"
     gene            complement(2318..3823)
                     /locus_tag="VNQ33_02715"
     CDS             complement(2318..3823)
                     /locus_tag="VNQ33_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97042.1"
                     /translation="MASDPPARPAAEATCDGQRGRRRSAGWIAGALAASVLLRLPYLH
                     APLTVDEAGALTVARSWAAGQRLYLDTFVDRPQGVIVVFQRWDGLVGPSTTSVRALAI
                     LAGLSVVLGTAVAARVASGRWAAGAVAAWIVATVSSSAAIEGHAANGELLAGAATVPA
                     AAVGALVAARRIPPPWLAAAGALAAAGLTVKQSAFDVLAALAVWIAVAAWRGWLGRRA
                     AAAMAGWLALGAGAVLGAAAGHGATLGWDAYAYAVYGFRIHARSAIAGPQGSRMAVTL
                     LVALPLLGPAAWLALRRLRTLDGPVSRRLRPEHVLVLLWIAVATAGFFAGGNYHRHYW
                     IQLAFPVAVASAVAITAGPRQTERDLVRTTALAVALPLAISAVLIARPSLERDPRVAA
                     DAAIARWYRQHRPSPDAGLLPLCASVTLYVDAGQLPRTPYLWVDHVRSGRGAIPKLVA
                     LLDGPDRPTYLAMHNPARRCDPTGRLGRAIARHYRAAATVDGVDVLEATPP"
     gene            complement(3883..4893)
                     /locus_tag="VNQ33_02720"
     CDS             complement(3883..4893)
                     /locus_tag="VNQ33_02720"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HWJ97043.1"
                     /translation="MTATLPSPSTSAPTGRPARKLPGLLPRLNRQSVEKRSDAYLDID
                     WDDPELQIGPDDPRCGLFEFDPLAATEWYRSQPVEVQRQVGLHRICSAFKVGMQFENL
                     LQRGLLSYAFRLPNGAPEYRYLHHEIIEESQHSLMFQELVNRSGMPVHGMPLWLRTTA
                     TVMVPVFQRLLPEQFFILVLGGEDPVDYLQRRQLREGTTHPLIEQIMKIHITEEARHI
                     AFARTYLKDLVPRLGGFRRTMLSIRTPIVLWIMAPLMVDPSSELHRAHGVPKEVLRQA
                     RRSEAGRQLRRESVAKLRRLCRDLGLMNPLSRFVWRRCGLWEDDGAPPAPADAPSDAV
                     AA"
     gene            complement(5060..>5613)
                     /locus_tag="VNQ33_02725"
     CDS             complement(5060..>5613)
                     /locus_tag="VNQ33_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HWJ97044.1"
                     /translation="RPLWDFPDGLFRREVAAYELASAIGWDVVPPTVGRGGPFGEGSV
                     QLFIDADFEQHHFTLVEDESNHPQLMRMCAFDIVANSTDRKGGHVLVDGAGHLWGIDN
                     GLCLHAEFKLRTVIWEFGGEPLPADLADDLVAFLDRPLPEPLAALLDPLERDALRTRA
                     RALVAEGRFPVDPTGHRVPWPLV"
BASE COUNT          821 a         1924 c         2160 g          708 t
ORIGIN      
        1 gaggccgccc ccgagcgcga agccggcgag gtcatcgaag acgacggcga agcccacctg
       61 aagatcgtcg agttcctcga cggcctcaag gtcctctgac ccaaccactc gaaaggaacg
      121 attgacatgg ctctttccaa ggtttgggtc cacgcggaac tgacccccga aggcaacgtg
      181 gcgccgatct cgctcgagat cctcgccaag gcccgcgaga tcgcggacac cgtcgaggtc
      241 ttcaccggct ccgacggctc cgccgttgcg gccaccgccg gcgcccacgg cgccaccaag
      301 gtgctcgcca ccggcgacct gagcggcaag ctccagggcg tgcacgtggc cgccgcgctc
      361 gccgcggcca tcgagggcgg cgacgtgccg gacctcatct tggccggcac cacctacgac
      421 ggccgcgaca tcgccgcccg cctctcggtg aagctcgaca agcccgtcct caccaacaac
      481 atcgacgtcg acgtcaccga cggcatcgtc gtgaccaccc ccatcttcgg tggcacgcag
      541 ctggtcaaga cgaagttcgc cgccggtgcg cccgccatcg ctctcttccg cccgaagtcc
      601 ttcgtggccg aggagtccgg cggcggcgcc gccgaggtgg ccaccatcgc cgtgcccgac
      661 accggtgcgg ccggtgcggc caagttgctc aaccgccacg tcgaggagac caccggtccc
      721 aagctcgacg aggccgccgt ggtcgtggcc ggtggccgtg gcctgggcga ggctgccaag
      781 ttcgagctca tcgaggacct ggccaagacc ctcaagggtg cgcccggcgc ctcgcgtgcc
      841 atcgtcgacg ccggctgggt gccctacagc taccaggtcg gccagaccgg caaggtcgtg
      901 aagcccaccg tctacatcgc tgcgggcatc tccggtgcca cccagcacat ggtgggcatg
      961 aagggctcca agaacatcat cgcgatcaac aaggacccgg aggccccgat cttcggcgtc
     1021 gccgacctcg gcatcgtggg cgacgtgcac aaggtgctgc ccaagctcct cgaagccctg
     1081 caggcccgct gatctgagac cacacgtgtg tacgagtggg ccccggccga agggccgggg
     1141 cccgttcgcg tcacgggggc ggcggtgagc ctccgggacc ggctgcgcaa ccgcaggaac
     1201 gacggcgccc tccgagccaa gctcagccag ggcgggccgg tgcgcgtccc gtgccggctc
     1261 cggcgcacct ccgcgcgtgg ctgggggccg tggacggagg gtcgggtgga cctgggcgcc
     1321 atccccgacg gcgaggcccg gtggagcacc gacgacgccg tggcggtggg cttcgcctcg
     1381 accaacccgc gggtggacct gccgttcgag gaggtgtcgg atgtctacct ccgggcggtc
     1441 cggttccgca ccgaggcgtt ctgggggatg gagaacgaca tcgtcgtggt ggtgagcgag
     1501 cggggcacga tcgaggtcgc ggtgacgccg ggcgatgccg aggccgtcgc tgcccgcctc
     1561 gccgaactgc tccagccagc cctctgacct gggccgcggt cggccggctg atcctctggc
     1621 cgcggtagcg tgcccgtcat gaccggcgag gatctgacca tcgaccagct cgccgccgag
     1681 gtcgacatga cggtgcgcaa catccgggcg caccaggcgc gcgggctgct gcccccgccc
     1741 accatcaagg gccgcatcgg ctactacggc ccgctccacc ggcgccggct gcagcagatc
     1801 cgggcgatgc aggacgaggg cctgaacctg gcggccatcg ccaaggtcgt caacggcggc
     1861 cagctcaccg cggtcacgac cgaggcgttc accgtgtccg agccggcgtg gttcgagccc
     1921 caggagctgc tgacgcggct cggcgatccc gatgacgccg tgacggcgcg ggccatcgac
     1981 ctcgggctga tcgtgctcga cggcgatcgc gtgcggatgg acatgcccgg tctgctcccg
     2041 ctggccgagg agttcctcgc cctgggcgtg ccgctggccg ctcagctcga cgccctcgcc
     2101 ggggtgcaag cggccaccga gcaggtggcg gcggcgtacc tgcagctcgc ggacgagcac
     2161 ctcatcgccc gcgtggcggt ggactccggc ggtgagctgg aggcgatccg ggcatcggtc
     2221 gcccgcctcg gagacctggc ccgggtggca ctggatgcgt cgttcaacca cgccatgacc
     2281 gaagcgctgc gcacgtactt cgagcagtag gcgcgggtca cggcggcgtc gcctcgagga
     2341 cgtcgacccc gtcgacggtg gccgccgcgc ggtagtggcg ggcgatggcc cggccgaggc
     2401 ggccggtcgg gtcgcagcgc cgtgccgggt tgtgcatcgc caggtaggtc gggcggtcgg
     2461 ggccgtcgag gagggccacg agcttgggga tcgccccgcg tcccgaacgg acgtggtcga
     2521 cccagaggta cggggtgcga gggagctggc cggcatcgac gtacagggtc acgctggcgc
     2581 acagggggag caggccggcg tcgggcgacg ggcggtgctg gcggtaccag cgggcgatgg
     2641 cggcgtcggc ggcgacgcga ggatcgcgct cgagggaggg gcgggcgatg agcaccgccg
     2701 agatggcgag ggggagggcg acggcgagcg cggtggtccg caccaggtcc cgctcggtct
     2761 ggcgcggacc cgcggtgatg gcgaccgcgc tcgccacggc gacgggaaac gccagctgga
     2821 tccagtagtg gcggtggtag ttgccgccgg cgaagaagcc cgcggtggcg acggcgatcc
     2881 acagcagcac caggacgtgc tcgggccgga ggcgacggct cacggggcca tcgagggtgc
     2941 ggaggcgccg cagggcgagc cacgcggcgg ggccgagcag cgggagggcg acgaggaggg
     3001 tgacggccat gcggctgccc tgggggccgg cgatggcgct gcgcgcgtgg atccggaagc
     3061 cgtacacggc gtaggcgtac gcatcccacc cgagcgtggc gccgtgcccg gcagccgcgc
     3121 ccagcacggc cccggcgccg agggcgagcc agccggccat cgctgccgcg gcccggcgcc
     3181 ccagccagcc ccgccacgcg gcgacggcga tccagacggc gagcgctgcg aggacgtcga
     3241 aggcggactg cttgaccgtg agcccggcgg ctgcgagcgc gccggcggcc gcgagccacg
     3301 gcggcgggat ccggcgggcg gccacgaggg cgccgaccgc cgccgcgggg acggtggcgg
     3361 cgccggcgag gagctcgccg ttggcggcgt ggccctcgat ggccgccgat gacgacacgg
     3421 tcgcgacgat ccacgcagcc acagcaccgg cggcccaccg gcccgaggcg acccgggccg
     3481 ccacagcggt gccgagcacc accgacaggc cggcgaggat cgccagggcg cgcacggacg
     3541 tcgtgctggg gccgacgaga ccatcccagc gctggaacac cacgatcacg ccctgcgggc
     3601 ggtcgacgaa ggtgtcgagg tagaggcgct gtccggcggc ccagctccgg gcgacggtga
     3661 gggcaccggc ctcgtcgacg gtcaggggcg cgtgcaggta cggcaggcgg agcaggaccg
     3721 aggcggccag cgctccggcg atccagccgg cggagcgccg ccgtccacgt tgcccgtcgc
     3781 aggtcgcttc ggcggcgggc ctcgccggtg ggtcgctcgc catgggaccc caacgctacc
     3841 gacggggtcg gtggcaccgg gacgcccgga tgccgcgtgg gatcaggcgg cgacggcgtc
     3901 tgagggtgcg tccgccgggg ccggcggggc gccgtcgtcc tcccagaggc cgcagcgccg
     3961 ccagacgaac cgtgagagcg ggttcatgag gccgaggtcc cggcagagcc ggcgcagctt
     4021 ggccaccgac tccctgcgca gctggcgtcc cgcctcgctg cgccgtgctt ggcggaggac
     4081 ctccttcggc acgccgtggg cccggtgcag ctcgctggac ggatccacca tgagcggcgc
     4141 catgatccag agcacgatcg gcgtgcggat cgacagcatc gtgcggcgga agccgcccag
     4201 ccggggcacg aggtccttga ggtaggtccg ggcgaaggcg atgtggcgcg cctcctcggt
     4261 gatgtggatc ttcatgatct gctcgatgag cgggtgggtg gtgccctcgc gcagctggcg
     4321 gcgctggagg tagtcgaccg ggtcctcgcc gccgaggacg aggatgaaga actgctccgg
     4381 caggaggcgc tggaacaccg gcaccatcac ggtggcggtg gtgcgcagcc aaagcggcat
     4441 cccgtggacg ggcatgccgc tgcggttcac cagctcctgg aacatgagcg agtgctggga
     4501 ctcctcgatg atctcgtggt gcaggtagcg gtactcgggg gcgccgttcg gcaggcggaa
     4561 ggcgtaggag agcaggccgc gctggaggag gttctcgaac tgcatgccga ccttgaacgc
     4621 gctgcagatg cggtggaggc cgacttggcg ctggacctcg acgggctgcg agcggtacca
     4681 ctcggtggcg gccagtggat cgaactcgaa gagcccgcag cgggggtcgt cggggccgat
     4741 ctggagctcg gggtcgtccc agtcgatgtc gaggtacgca tcggagcgct tctcgacgga
     4801 ttggcggttg agccggggga gcagtccggg cagcttgcgg gccgggcgcc cggtcggggc
     4861 gctggtgctc ggggagggga gggtcgccgt catcggatca gctcctgggg gatggggcgg
     4921 gatgcggtca tcgtctcatg tgacatcaaa cgatgtcaac gttgcgcccg tcacaccatg
     4981 ccgctgacct gcggcttcgt gcccgggacc gtgctcggag agccgaggcg ggcggagcgg
     5041 acccagacga ggagcggact cagacgaggg gccagggcac ccggtggccg gtggggtcca
     5101 ccgggaaccg gccctcggcc accagcgccc tcgcccgcgt ccgcagggca tcgcgctcga
     5161 ggggatcgag cagcgccgcg aggggctcgg gaagcgggcg gtcgaggaac gcgacgaggt
     5221 cgtcggccag gtccgcaggc aggggctcgc cgccgaactc ccagatcacc gtgcgcagct
     5281 tgaactcggc gtggaggcac agcccgttgt cgatccccca gaggtggccg gcgccgtcga
     5341 ccaggacgtg gccgcccttg cggtcggtgc tgttggcgac gatgtcgaac gcgcacatcc
     5401 gcatcagctg gggatggttc gactcgtctt cgacgagcgt gaagtggtgc tgctcgaagt
     5461 cggcgtcgat gaacagctgc accgatccct cgccgaacgg cccgcctcgt ccgacggtgg
     5521 gaggcacgac gtcccacccg atggcactgg ccagctcgta ggccgcgacc tcgcgccgga
     5581 agaggccgtc ggggaagtcc cacagcgggc gct
//
LOCUS       DATMFS010000148         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_215242, whole genome shotgun sequence.
ACCESSION   DATMFS010000148 DATMFS010000000
VERSION     DATMFS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215242"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VNQ33_02730"
     CDS             complement(<1..360)
                     /locus_tag="VNQ33_02730"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440660.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWJ97045.1"
                     /translation="MTGALPRADVGVFGGSGFYRFLDDVTELSVDTPYGPTAAPVHLG
                     TLGDRTVAFIPRHGAHHEFAPHRVPYRANVWAMHALGVTSVFGPCASGSLQARIAPGS
                     FVVPDQLVDRTSGRPDTF"
     gene            complement(365..745)
                     /locus_tag="VNQ33_02735"
     CDS             complement(365..745)
                     /locus_tag="VNQ33_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWJ97046.1"
                     /translation="MPTYEYRCKHCGHEFDIVQSFSEDSLTECPSCHEQTLKKVFGNV
                     GITFKGSGFYKTDSRSAGSFGSTRDADKSDNGSGSSTSTATKDTKETSSTSSGSGSSS
                     DSSSSSSASPASGSTSTSSSSTPA"
     gene            885..1784
                     /gene="galU"
                     /locus_tag="VNQ33_02740"
     CDS             885..1784
                     /gene="galU"
                     /locus_tag="VNQ33_02740"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWJ97047.1"
                     /translation="MTTPRSAVRKAVIPAAGLGTRFLPATKAQPKEMLPVVDKPAIQY
                     VVEEAVAAGCDDILIVTGRSKRSIEDHFDRQFELEYHLDKSGKLDELGEVVDLAELAD
                     IHYVRQGEPLGLGHAISVAQKHVGDNPFAVLLGDEFMAKDSTLLSDMIKIHTQYGRSV
                     LALQEVEEADIKRYGNVKPERISDQLVQILDLVEKPDPAVAPSNLAVMGRYVFTPEIF
                     DKLEQVEPGVGGEIQLTDAIALLLADQAVYGATFKGGRYDTGTIPAYLETIVELALQR
                     DDIGPEFRRFLADICERHDLHAT"
     gene            1795..3015
                     /gene="glp"
                     /locus_tag="VNQ33_02745"
     CDS             1795..3015
                     /gene="glp"
                     /locus_tag="VNQ33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWJ97048.1"
                     /translation="MIPLAEAREHVLSRCAPRAPLSVPLSDALGCVLAGPVTAGEPVP
                     PWANSAMDGYAVRSADVASASEATPVSLPVVATVAAGAAADTEVGAGQAVRIMTGAPM
                     PLGADAVVMVERTRGGPVAAIVEVLDPVEHGRNVRAAGSDIAPGQLVADVGTVLGPAH
                     LGVLASVNVRRPIVVPRPRLGIISTGDELVDDGSPLEPGQIRESNRPMLVALARTFGV
                     DAVDLGTVADDRDALATALAGAAATCDAVATSGGVSMGDFDLVKVVLGELADMRWMQV
                     AIRPAKPFAFGVLDGTPVFGLPGNPVSSMVSLALLGVPGLRRLAGRSDLDLPRVAAVA
                     GPGLGRRPDGRTAYQRVSCRWVDGRLHAHPVTGQGSHQLASAAGANALAELPDGDGVA
                     EGDPVTVVLLAPPL"
     gene            3179..4180
                     /gene="moaA"
                     /locus_tag="VNQ33_02750"
     CDS             3179..4180
                     /gene="moaA"
                     /locus_tag="VNQ33_02750"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWJ97049.1"
                     /translation="MTDLAVPTRPLADGFGRVHRDLRISVTDRCNFRCTYCMPAEGMD
                     WHPRHELLTFEELARVAAVCVEHFGFDSIRLTGGEPTVRAGLPVLVEQLAALPVDLSL
                     TTNGSTLRTQARDLKAAGLGRLNISLDSLHVDRFAALTRRDELPRVLDGIRAAVDAGF
                     DPVKVNVVVVRGVNDDEIVDFARFGRDEGVTVRFIEFMPLDADHRWSPGQVVPSAEIV
                     AAVDAVFPTEPVRRGSEPAERHRYLDGAGEIGVIGTVTEPFCSSCDRVRLTADGQLRA
                     CLFATDETDLRTLLRGGATDDDLAVAVAATVAGKWAGHGISNVTFTRPTRSMSEIGG"
BASE COUNT          602 a         1636 c         1558 g          672 t
ORIGIN      
        1 gaaggtgtca ggtcgtcctg acgtgcggtc gaccagctga tcgggcacca cgaagctgcc
       61 gggggcgatg cgggcctgga gcgatccgct ggcgcacggg ccgaacacgc tggtgacgcc
      121 gagggcgtgc atggcccaca cgttcgcccg gtacgggact cggtgcgggg cgaactcgtg
      181 gtgcgcgccg tggcggggga tgaacgcgac cgtccggtcg cccagggtcc cgaggtggac
      241 cggcgctgcg gtcgggccgt agggcgtgtc gacgctgagc tcggtgacgt cgtcgaggaa
      301 ccggtagaac ccggacccgc cgaacacgcc gacgtcggca cgggggaggg cgccggtcat
      361 cgccctacgc cggcgtcgac gagctggagg tcgacgtgga gcccgaggcc ggcgaggccg
      421 agctgctgga gctcgaatcg ctggagctgc ccgagcccga cgaggtcgag ctcgtctcct
      481 tcgtgtcctt cgtggcggtc gaggtcgacg agccggaccc gttgtcgctc ttgtccgcat
      541 cgcgggtgga cccgaacgag ccagccgagc ggctgtcggt cttgtagaag cccgagccct
      601 tgaacgtgat gccgacgttg ccgaacacct tcttcagggt ctgctcgtgg caggacgggc
      661 actcggtgag cgagtcctcg gagaacgact gcacgatgtc gaactcgtgg ccgcagtgct
      721 tgcagcggta ctcgtaggtg ggcatcggtc ctcgctggcc aagatcggcg gttagcagtc
      781 tcaatcggag actgccagtg tagggaagca ggcggggatc gggcacatgc agaggggcgc
      841 cgcgtggccc gggcgacctc ggtccgctgc gcgagactgc agccgtgacc accccacgat
      901 ccgcagtgcg caaggccgtc atccccgccg ctggcctcgg cacccgcttc ctcccggcca
      961 ccaaggcgca gccgaaggag atgctgcccg tcgtggacaa gcccgccatc cagtacgtgg
     1021 tggaggaggc tgtcgccgcc ggctgcgacg acatcttgat cgtcaccggc cggtcgaagc
     1081 ggtcgatcga ggaccacttc gaccgccagt tcgagctcga gtaccacctg gacaagtcgg
     1141 ggaagctcga cgagctgggc gaggtggtcg acctggccga gctggccgac atccactacg
     1201 tccgccaggg tgagccgctc gggctcggcc acgccatctc cgtcgcccag aagcacgtcg
     1261 gcgacaaccc cttcgccgtc ttgctgggcg acgagttcat ggcgaaggac tcgacgctcc
     1321 tgtcggacat gatcaagatc cacacgcagt acggccgctc ggtgctcgcc ctccaggagg
     1381 tggaggaggc cgacatcaag cgctacggca acgtgaagcc cgagcgcatc tccgatcagc
     1441 tcgtccagat tctcgatctg gtcgagaagc ccgatccggc cgtggccccg tcgaacctcg
     1501 ccgtgatggg ccgctacgtc ttcaccccgg agatcttcga caagctcgaa caggtggagc
     1561 ccggcgtcgg cggcgagatc cagctcaccg acgcgatcgc gctgctcctc gccgaccaag
     1621 ccgtgtacgg agcgacgttc aagggcggcc gctacgacac cggcaccatc ccggcgtacc
     1681 tcgagaccat cgtcgagctc gccctccagc gcgacgacat cggtccggag ttccgccggt
     1741 tcctcgccga catctgcgag cgccacgacc tccacgccac ctgatcgggt gccgatgatc
     1801 ccgctggccg aggcgcgcga gcacgtcttg tcgcggtgcg cgccccgggc gccgctgtcg
     1861 gtgccgctgt ccgacgcgct gggctgcgtg ctggccgggc cggtgaccgc cggcgaaccg
     1921 gtcccgccgt gggcgaactc ggccatggac ggctacgccg tgcgatcggc cgacgtggcg
     1981 agcgcgtccg aggcgacgcc ggtgtccctg ccggtcgtgg ccacggtcgc tgccggtgcc
     2041 gccgccgaca ccgaggtcgg tgccgggcag gcggtgcgga tcatgaccgg cgccccgatg
     2101 cccctcgggg ccgatgccgt ggtgatggtc gagcggaccc gcggcgggcc cgtcgccgcc
     2161 atcgtcgagg tcctggaccc ggtggagcac ggacgcaacg tccgggcggc aggcagcgac
     2221 atcgctccgg gtcagctcgt cgccgacgtc gggaccgtcc tcggtccggc gcacctgggg
     2281 gtcctcgcgt cggtgaacgt ccgccgcccg atcgtggtgc cccgccctcg cctcgggatc
     2341 atctccaccg gcgatgagct ggtcgacgac ggcagcccgc tcgaaccggg ccagatccgc
     2401 gagagcaacc ggccgatgct cgtcgccctc gcccgcacgt tcggcgtcga tgccgtcgac
     2461 ctcggcacgg tggccgacga ccgcgacgcc ctcgccacgg cgctggccgg cgccgccgcc
     2521 acttgcgatg cggtcgccac cagcggtggg gtgagcatgg gtgacttcga cctggtgaag
     2581 gtcgtgctcg gcgaactggc tgacatgcgc tggatgcagg tggcgatccg tccggccaag
     2641 ccgttcgcct tcggcgtgct cgacggcacg ccggtcttcg gcctgcccgg caaccccgtc
     2701 tcctcgatgg tgtcgctcgc cctgctcggc gtgcccggtc tccgccgtct ggcggggcgg
     2761 tccgacctcg acctgccgcg ggtcgcggcg gtggcgggcc cgggcctggg tcgccggcct
     2821 gacggtcgca ccgcctacca gcgggtctcg tgccgctggg tcgacggccg tctgcacgcc
     2881 caccccgtca ccggccaggg ctcccaccag ctcgcgtcgg cggccggcgc caacgccctc
     2941 gccgagctcc ccgacggcga cggcgtggcc gaaggcgacc ccgtcaccgt cgtcctcctc
     3001 gccccacccc tctgatcagg gccacgacac cgtggcatcg gcgggcttgc gccccgagtc
     3061 ggctggtggt ccggtcgtcg ccaggtatcg gggccagaac cccgtcgatg ccgcaggttc
     3121 gcgacccaca tcgctggcaa ggggaccgtt gctgccggcg gctcgtagcc tgggccgagt
     3181 gaccgatctc gccgtgccga cgcgcccctt ggccgacggc ttcggccgcg tccaccggga
     3241 cctgcggatc tcggtcacgg atcgctgcaa cttccggtgc acctactgca tgccggcaga
     3301 gggcatggac tggcatccgc gccatgagct gctgacgttc gaggagctgg cccgggtggc
     3361 cgccgtgtgc gtcgagcact tcgggttcga ctcgatccgt ctcacgggag gcgagcccac
     3421 cgtgcgggcc gggctcccgg tactcgtcga gcagctcgcc gccctgcccg tcgatctctc
     3481 gctcaccacc aacggctcca ccttgcggac ccaggcccgc gacctgaagg cggccgggct
     3541 cggacgcctc aacatctcgc ttgactcctt gcacgtcgac cgcttcgcgg cgctgacccg
     3601 tcgcgacgag ctgccgcgcg tgctcgacgg catccgtgct gcggtcgacg ccgggttcga
     3661 cccggtgaag gtgaacgtgg tcgtggtgcg aggcgtgaac gacgacgaga tcgtcgactt
     3721 cgcccgcttc ggccgcgacg agggcgtgac cgtccgcttc atcgagttca tgcccctcga
     3781 cgccgaccac cggtggagcc ccggccaggt ggtcccgtcc gcggagatcg tcgccgccgt
     3841 cgacgccgtc ttccccaccg agccggtccg ccgcggctcg gagccggcgg agcgccaccg
     3901 gtacctcgac ggtgccggcg agatcggcgt catcgggacc gtcaccgagc cgttctgctc
     3961 cagctgcgat cgggtccggc tcaccgccga cggccagctg cgcgcctgcc tcttcgccac
     4021 cgacgagacc gacctgcgca ccctgctccg cggcggtgcc accgacgacg acctggccgt
     4081 tgccgtcgcc gccacggtgg cgggcaagtg ggctggccac ggcatcagca acgtcacctt
     4141 caccaggcca acgcggtcca tgtccgagat cggcgggtag ggcccgcgcc gcacgcccgc
     4201 tcgttcgggt tgcgccttgc gccgtcctcg aaaccggagg agggcctgac ggccagcagc
     4261 tcggcacggc tgcgtcgcat ccgctcgcgt tcctggggac tccgcatgct ccgtcccgtc
     4321 cccctcaacc cctgggctcg tgcactcgga acccgttctt cgcgccgggt cctcggtcgt
     4381 cgtcggagac gccggcgacc aggtgacgag atgggcacac ctagacttcc gccatgcctg
     4441 agcgcctcac ccacctcgat ccgctggg
//
LOCUS       DATMFS010000149         4335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_98976, whole genome shotgun sequence.
ACCESSION   DATMFS010000149 DATMFS010000000
VERSION     DATMFS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98976"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="hrcA"
                     /locus_tag="VNQ33_02755"
     CDS             complement(<1..625)
                     /gene="hrcA"
                     /locus_tag="VNQ33_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWJ97050.1"
                     /translation="MALGRIECQPDPCEVSVLDERKASILRAVVEEYIQTAQPVGSGH
                     IASAPGVQVSSATVRNEMAALESEGFLAQPHTSAGRIPTEKGYRYFVDALTQPSLDRT
                     ATQQVRAFFDQAHGEIEQMLADTTRLLTGLTDYAAVVVGPAHEAAAVRSVQLVSLAPS
                     VALFVAVLGNGAVEKRTIELGDPVDESVLAAATAALVAGADGQPLAVL"
     gene            complement(638..2422)
                     /gene="lepA"
                     /locus_tag="VNQ33_02760"
     CDS             complement(638..2422)
                     /gene="lepA"
                     /locus_tag="VNQ33_02760"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWJ97051.1"
                     /translation="MDQSRIRNFSVIAHIDHGKTTLSDRILEVTGAVSARDMRAQFLD
                     SMDIERERGITIKLQSVTLGFQGHTLNMIDTPGHVDFGYEVSRSLAACEGVILLVDAA
                     QGIEAQTLANCYLALENDLEIVACLNKIDLPAADPDLYAAEIENVLGIPAEDILRISA
                     KTGEGVPELLEAVIERIPAPQGDVDEPLQALIFDSHFDQFRGVVSSVRVVNGTMRTND
                     KLKFMQAGVTYEADEIGVRNPVNSPVKELGPGETGYLIAGIKDVAEARSGETVTTARN
                     GAEAALDGYRDPKPMVFCGIYPIDGDQFPDLREALDKMRLSDASVTFEPETSGALGFG
                     FRCGFLGLLHMEIIRERLEREFDLSLIATAPSVAYLVNKTNGDQVEVDNPSEMPPVTE
                     IESISEPWFDVTILTPSDYTGTIMDLCQTRRGEMDKLEYLSPERVELKYKIPLAEVVT
                     DFFDQLKSRTQGYASLDYEPAGYQVSKLVRVDVLLNATPVDAFSMVIHKDKADDYGRK
                     MCEKLRELIPRQMFDVPIQAAIGGRIIARQTVKAKRKDVLAKCYGGDITRKRKLLEKQ
                     KEGKKKMKNIGRVEVPQEAFVSALKLDD"
     gene            complement(2493..2747)
                     /locus_tag="VNQ33_02765"
     CDS             complement(2493..2747)
                     /locus_tag="VNQ33_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97052.1"
                     /translation="MTIAESRRLALFESAKASWGADTAQTLMDLLPPDPDALATKADL
                     AVLGAELRAEMAGQTRTLVLAMLTIEVTIVLAVLGAVVAR"
     gene            2899..3168
                     /gene="rpsT"
                     /locus_tag="VNQ33_02770"
     CDS             2899..3168
                     /gene="rpsT"
                     /locus_tag="VNQ33_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWJ97053.1"
                     /translation="MANIKSQIKRNRQNEKRRVRNKTVRSELRTRTKTAVSAAESGAE
                     NAAEAERLAMKRIDKAATKGVIHKNAAARRKSRLAKRLAAASAEA"
     gene            complement(3288..>4335)
                     /gene="holA"
                     /locus_tag="VNQ33_02775"
     CDS             complement(3288..>4335)
                     /gene="holA"
                     /locus_tag="VNQ33_02775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWJ97054.1"
                     /translation="SPCPGGRLVTARLPVYLVKGSDPVLLGGAVSNLLEQLVDGGDRS
                     LLVDEFDLDTTKLAAALDAAQTIPFLTDFRIVVVRRFGRYSKGDDVAPVVAYLEDALP
                     SSTLVLVGEKTALTSANEDGEPFTKTPKMPPALTKAVTAAGGEVIETDAAARNMSGWV
                     GEQFAAAGLDVEPAVRDKVVKTIGEDGGAVVGIIERLKGAFTPGERLRLADVEPFLGP
                     PGGVPPWDLTDAIDKGDVTLALDRLQRMMGAGERHSLAIMATLQSHYLRMAKLDGANV
                     RGEKEAAAILGMKGSTFPAKKAMNQARKLGSPGIRRAVALLSKADVDLRGAQAWPQEL
                     TMEVLVARLARNSR"
BASE COUNT          641 a         1424 c         1574 g          696 t
ORIGIN      
        1 cgaggacggc gaggggctgg ccgtcggccc cggcgaccag cgcggccgtc gccgcggcga
       61 gcacggactc atcgacgggg tcacccagtt cgatcgtgcg cttctcgacg gccccgttgc
      121 cgaggaccgc gacgaagagg gcgaccgagg gggccagcga caccagctgc acggagcgga
      181 ccgccgccgc ctcgtgggcg gggccgacca ccacggcggc gtagtcggtg aggcccgtca
      241 gcagccgggt ggtgtccgcg agcatctgct cgatctcgcc gtgggcctgg tcgaagaagg
      301 cgcggacctg ctgggtggcc gtgcggtcga gcgacggctg ggtgagcgcg tcgacgaagt
      361 accggtagcc cttctcggtg gggatccgtc ccgccgacgt gtgcggctgg gccaggaacc
      421 cctcactctc gagcgccgcc atctcgttgc gcaccgtggc tgacgagacc tgcaccccgg
      481 gcgcggacgc gatgtggccg gagccgacgg gctgagccgt ctggatgtac tcctccacca
      541 cggcacggag gatggatgcc ttgcgctcgt cgagcacgga gacctcgcag ggatcgggtt
      601 ggcactcgat cctaccgagt gccaaccctg ccgagcatca gtcgtcgagc ttgagggcgg
      661 agacgaaggc ttcctgggga acttcgacgc ggccgatgtt cttcatcttc ttcttgccct
      721 ccttctgctt ctcgaggagc ttgcgcttgc gggtgatgtc gccgccgtag cacttggcca
      781 gcacgtcctt gcgcttggcc ttcacggtct ggcgggcgat gatccggccg ccgatggccg
      841 cctggatcgg cacgtcgaac atctggcgcg ggatgagctc gcggagcttc tcgcacatct
      901 tgcggccgta gtcgtcggcc ttgtccttgt ggatcaccat ggagaaggcg tcgaccggcg
      961 tggcgttgag cagcacgtcg acgcggacca gcttcgacac ctggtagccg gccggctcgt
     1021 agtcgaggct ggcgtagccc tgggtgcggc tcttgagctg gtcgaagaag tcggtgacga
     1081 cctcggcgag cgggatcttg tacttgagct ccacccgctc gggcgacagg tactcgagct
     1141 tgtccatctc gccccgccgg gtctggcaga ggtccatgat cgtgcccgtg tagtcggacg
     1201 gggtgaggat cgtgacgtcg aaccacggtt cggagatgct ctcgatctcg gtcaccggcg
     1261 gcatctccga cgggttgtcg acctcgacct gatccccgtt ggtcttgttg accaggtagg
     1321 cgacgctcgg ggcggtggcg atgaggctca ggtcgaactc gcgctcgagg cgctcccgga
     1381 tgatctccat gtggagcagg ccgaggaagc cgcaccggaa gccgaacccg agcgcacccg
     1441 aggtctccgg ctcgaacgtg acgctggcat cgctgaggcg catcttgtcg agcgcctcgc
     1501 gcagatccgg gaactggtcg ccgtcgatcg ggtagatgcc gcagaacacc atcggcttgg
     1561 gatcgcggta gccgtcgagg gcggcctcgg caccgttgcg ggccgtggtg accgtctcac
     1621 cggagcgggc ctcggccacg tccttgatgc cggcgatgag gtagccggtc tcgcccggcc
     1681 cgagctcctt caccggtgag ttgacggggt tgcgcacgcc gatctcgtcg gcctcgtagg
     1741 tgacgccggc ctgcatgaac ttgagcttgt cgttcgtgcg catggtgccg ttgacgaccc
     1801 ggacgctgga gacgacgccg cggaactggt cgaagtggct gtcgaagatg agcgcctgca
     1861 gcggctcgtc gacgtcgccc tgcggggcgg ggatgcgctc gatgaccgcc tcgagcagct
     1921 cgggcacgcc ctcgccggtc ttggcgctga tgcggaggat gtcctccgcc gggatgccga
     1981 ggacgttctc gatctccgcc gcgtagaggt ccgggtcggc ggccggcagg tcgatcttgt
     2041 tgaggcaggc gacgatctcg aggtcgttct ccagcgccag gtagcagttg gccagggtct
     2101 gggcctcgat gccctgagcg gcgtcgacga gcaggatcac gccctcgcac gcggcgagcg
     2161 accggctgac ctcgtagccg aagtccacgt ggcccggcgt gtcgatcatg ttgagcgtgt
     2221 ggccctggaa gcccagggtc accgactgga gcttgatggt gatcccccgc tcccgctcga
     2281 tgtccatcga atccaggaac tgggcccgca tgtcccgggc gctcacggcg ccggtgacct
     2341 cgaggatccg gtcgctcagg gtcgtcttcc cgtggtcgat gtgggcgatg acgctgaagt
     2401 tgcggatgcg ggactggtcc atggggcctt cgactgccga gctggtcaga cgacgctacc
     2461 aaccgggctg atgcacccca cagctgacca ggtcagcgtg cgacgaccgc tccgagcacc
     2521 gcgagcacga tggtgacctc gatcgtcagc atggcgagca ccagcgtccg tgtctgacct
     2581 gccatctcgg cacggagctc ggcaccgagc acggcgaggt ctgctttggt ggcgagggca
     2641 tcgggatcgg gagggagcag gtccatgagc gtctgggcgg tgtcggcgcc ccaggaggcc
     2701 ttggccgact cgaacagggc gagtcggcgg gattcggcga tggtcatcgt gttccttcgg
     2761 atgcgaattg gcaagggctg gacccaggcg ggccgaaggg ggtggtacgc cccatcgtag
     2821 aggcgggttg tgacagcgag gggtggcacc actacgctgg tccagccttc agaacgcaga
     2881 agtggagcac gagccaccgt ggccaacatc aagagccaga tcaagcgcaa tcgccagaac
     2941 gagaagcgcc gcgtccgcaa caagaccgtc cggtccgagc tgcgcacgcg caccaagacc
     3001 gccgtctcgg ccgccgagtc cggcgcggag aacgcggccg aggccgagcg cctcgccatg
     3061 aagcggatcg acaaggccgc caccaagggc gtgatccaca agaacgcggc ggcccgccgc
     3121 aagtcccgcc tggccaagcg cctggcggct gcttccgccg aagcctgatc ctccggccgg
     3181 tccgccggcc acccgacccg accccgctca ccgcacggtg acggggtcgt ttcgcgttcg
     3241 ggcacctgtg gcgctggacc cggctcagcg cgcgtcggcg aggccgctca gcgggagttg
     3301 cgggcgaggc gggcgacgag gacctccatc gtcagctcct gcggccaggc ttgggcgcca
     3361 cggaggtcga cgtcggcctt cgacagcagg gccacagccc gccggatacc cggcgagccc
     3421 agcttgcggg cctggttcat ggccttcttc gcgggaaagg tcgagccctt catcccgagg
     3481 atcgccgccg cctccttctc gccgcgcacg ttggcgccgt cgagcttcgc catccgcagg
     3541 tagtgcgact ggagggtggc catgatggcc agcgagtggc gctcgccggc gcccatcatc
     3601 cgctggagcc ggtcgagtgc gagggtgacg tcgcccttgt cgatggcatc ggtgaggtcc
     3661 cacggcggaa cgccgccggg cgggccgagg aagggctcga cgtcggccag gcgcagccgt
     3721 tcaccggggg tgaaggcgcc cttcaggcgc tcgatgatgc cgacgacagc accgccgtcc
     3781 tcgccgatgg tcttcaccac cttgtcccgc acggcgggct cgacgtcgag gccggccgcg
     3841 gcgaactgct cacccaccca accggacatg ttgcgggcgg cggcgtcggt ctcgatgacc
     3901 tcgcctcctg ctgccgtgac cgccttggtg agcgccggcg gcatcttggg cgtcttggtg
     3961 aacggctcgc cgtcctcgtt ggccgaggtg agcgccgtct tctccccgac gaggacgagc
     4021 gtcgacgacg gcagggcgtc ctcgaggtag gcgacgacgg gcgcgacgtc gtcgcccttg
     4081 gagtagcggc cgaagcggcg gaccaccacg atgcggaagt cggtgaggaa cgggatcgtc
     4141 tgcgcggcat ccagggcggc ggcgagcttg gtggtgtcga ggtcgaactc gtcgacgagc
     4201 agggagcggt cgcccccgtc gacgagctgc tcgagcaggt tggacacggc gccgcccaac
     4261 aggacggggt cgctcccctt cacgaggtac accggcaggc gggcggtcac gaggcgtcct
     4321 ccggggcagg ggctc
//
LOCUS       DATMFS010000150         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_52007, whole genome shotgun sequence.
ACCESSION   DATMFS010000150 DATMFS010000000
VERSION     DATMFS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52007"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..752)
                     /locus_tag="VNQ33_02780"
     CDS             complement(144..752)
                     /locus_tag="VNQ33_02780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ97055.1"
                     /translation="MELWLIRHGLPLRIDGGDGPADPPLSPEGTEQADLMARWWSRHG
                     IDAVYASPMRRAHETAVPLAAATGHEITLDDGLREFDAHLNFYVPIEELRADEEAWKR
                     LVEEWLSPEAEAKRQEFRKEVIASIDAIVASHPGERVAVVCHGGVINAYLSDIISLPG
                     TMWFEPAYTSVSRAMAGGTHKQVVSLNETPHLPHLIVPATVG"
     gene            792..1559
                     /locus_tag="VNQ33_02785"
     CDS             792..1559
                     /locus_tag="VNQ33_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ97056.1"
                     /translation="MPAPLDLHVVDTPSADPGAAVVVVVHGAMDRSSSFGRVARQLDD
                     LHVVRYDRRGYGRSVGAPVGTLDDHVDDLLGVIDGRAVTVFGHSIGGVIALVAAQRDP
                     GSIRSVLAYEAPSPWADWWPRPRTVPPDDSADEAEAFMRRAIGGHYWDRLPARTRSDR
                     RSEGAALRADIASLQGTSPFEASAIEAPVLAACGSDTTWWHQRATRELADAVPGGEHA
                     VVAGAEHAAHLTHPAATAHLVRRAAARAAGGTPDHRR"
     gene            1572..2501
                     /locus_tag="VNQ33_02790"
     CDS             1572..2501
                     /locus_tag="VNQ33_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWJ97057.1"
                     /translation="MSRIRDLLRRKPKRTAFVLSGGGNLGALQVGMVRALFERRIAPD
                     VILGCSVGALNGSAIASDPSLVMVGRLQEMWRSLADEGVMPAGVFPAVQLARKGVAIH
                     DNTNLRRVIESVLPGGRFEDLTVPFQCVATALVEGTETWFERGPLVDPIIASSAIPAV
                     YPPVEIDGVKYLDGAIVNDVPVSRAVALGCDRIFVLHVGAWDRPRPEPKRPIDMATYA
                     YWLARRSRFQRDLASIPSNIEVLVLPPGGTPVIKYNDFGHSDEMITSAYQATSAFLDA
                     VDADHANDLAAAVPGVRQEDRPRAVPTPDEPLP"
BASE COUNT          343 a          956 c          947 g          378 t
ORIGIN      
        1 cctacgtggc ggtcggccgc atcgacaccg cgatcgacgt gctcgaagac ctcaccgagc
       61 gcgtcgaccc ccgccgacgc gacgtgctcg tggcccgcat cgacacgttg cgggcgggac
      121 tgaactgacg gcgagggctc ccgtcagccc acggtggcgg gaacgatgag gtgggggagg
      181 tgcggcgtct cgttgaggct gacgacctgc ttgtgcgtcc cgccggccat cgcccggctc
      241 acgctggtgt aggccggctc gaaccacatc gtcccgggca gggagatgat gtcggagagg
      301 taggcgttga tgaccccgcc gtggcagacc accgcgaccc gttcgcccgg atggctcgcc
      361 acgatggcgt cgatgctggc gatgacctcc ttgcggaact cctggcgctt ggcctcggcc
      421 tcgggcgaca gccactcctc gaccaggcgc ttccacgcct cctcgtcggc gcggagctcc
      481 tcgatgggca cgtagaagtt gaggtgggcg tcgaactcgc gcaggccgtc gtcgagcgtg
      541 atctcgtgac cggtggcggc ggcgagcggc accgccgtct cgtgggcgcg gcgcatcggg
      601 ctggcgtaga cggcgtcgat gccgtggcgg gaccaccatc tcgccatcag gtccgcctgc
      661 tcggtgccct cgggggagag aggcggatcg gccgggccgt cgcctccgtc gatccgaagg
      721 ggcagtccgt gtctgatcag ccagagctcc acggcgcaga tggtaggcgt cggctgccta
      781 gggtcgacca cgtgcccgcc cctctcgatc tgcacgtggt cgacacaccg tccgctgacc
      841 cgggggcggc ggtcgtggtg gtggtccacg gggcgatgga tcgcagctcg tcgttcggcc
      901 gcgtggcccg ccagctcgac gacctccacg tcgtccgcta cgaccgccgg ggctacggcc
      961 gcagcgtcgg cgctccggtc ggcaccctcg acgaccacgt cgacgacctg ctcggcgtga
     1021 tcgacggccg cgcggtcacc gtgttcgggc acagcatcgg cggggtgatc gccctggtcg
     1081 cggcccagcg cgaccccgga tcgatccgtt cggtgctggc gtacgaggcg ccctccccgt
     1141 gggccgactg gtggccgcgg ccccgcacgg tgccacccga cgactcggcc gacgaggccg
     1201 aggcgttcat gcggcgagcc atcgggggcc actactggga ccggctgccg gcccgcaccc
     1261 gaagcgaccg ccgctcggag ggggcggcgc tgcgggccga catcgcctcg ctgcagggga
     1321 cctcgccctt cgaggcctcg gccatcgagg cgccggtcct cgccgcctgc gggtccgaca
     1381 ccacctggtg gcaccagcgg gcgacgcggg agctggccga cgccgtgccc ggtggcgagc
     1441 acgcggtcgt ggccggagcg gagcacgcgg cccacctgac ccatccggcc gccaccgcgc
     1501 acctcgtgcg gcgggcggcg gcccgagccg ccggcgggac ccctgaccac cggcggtagc
     1561 ctccgtccgg catgtcccgg atccgtgacc tcctccgacg caagccgaag cgcaccgcgt
     1621 tcgtcctgag cggcggcggc aacctcgggg cgctccaggt gggcatggtg cgggcgctgt
     1681 tcgagcggcg catcgctcct gacgtgatcc tgggctgctc ggtcggtgcg ctgaacggct
     1741 cggccatcgc ctcggatccg tcgctggtca tggtcggacg cctccaggag atgtggcgga
     1801 gcctggcgga tgagggcgtg atgcccgccg gcgtgttccc cgccgtgcag ctggcgcgca
     1861 agggcgtcgc gatccacgac aacaccaacc tccgccgggt gatcgagtcg gtcctccccg
     1921 gtggtcgctt cgaggacctc acggtcccgt tccagtgcgt ggcgacggcg ctcgtcgagg
     1981 gcacggagac ctggttcgag cgcgggcccc tggtggatcc gatcatcgcg tcgtcggcca
     2041 tcccggcggt gtacccgccc gtcgagatcg acggggtcaa gtacctcgac ggggccatcg
     2101 tcaacgacgt cccggtcagc cgggcagtcg cgctcgggtg cgaccgcatc ttcgtgctcc
     2161 acgtcggtgc ctgggaccgg ccccggcccg agcccaagcg gcccatcgac atggcgacct
     2221 acgcctactg gctggcccgt cggagccgct tccagcgcga cctggcgagc atcccctcga
     2281 acatcgaggt cctcgtcctc ccaccgggag gcaccccggt catcaagtac aacgacttcg
     2341 gccacagcga tgagatgatc acgagcgcgt accaggcgac gtcggccttc ctcgacgccg
     2401 tcgacgccga ccacgcgaac gacctcgccg cggccgtccc cggtgtccgc caggaggacc
     2461 gtccccgagc ggtcccgacg cccgacgagc cgctcccgta gctccccggt cccgggtggc
     2521 ggcacccgcg gaacgggtga ggcggggggc gaaggcgcag gtcagcgaag gggccgtttg
     2581 gcctattcag gtcctgagcg gagcgtccga tggattaccc gtca
//
LOCUS       DATMFS010000151         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_215446, whole genome shotgun sequence.
ACCESSION   DATMFS010000151 DATMFS010000000
VERSION     DATMFS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215446"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VNQ33_02795"
     CDS             <1..653
                     /locus_tag="VNQ33_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97058.1"
                     /translation="KPEVVEAIAAAGGEAVTVAADVRDFAQVEAAVAKTIEAFGGLNV
                     LYNNAGVSLGDDDDVASTPESTWELTMDVNVKGLWLCCKAALPALLDSGNASIINVAS
                     FVAHLGAATPQLAYTTSKGAVLSMTREIAVIYARQGIRANALCPGPVLTPLLAKYLSD
                     EEKRQRRLVHIPMGRFGTSEEMVNGALFLASDESSFMTGQSLLIDGGITAAYTTPE"
     gene            719..>2027
                     /locus_tag="VNQ33_02800"
     CDS             719..>2027
                     /locus_tag="VNQ33_02800"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWJ97059.1"
                     /translation="MERVTLTLDALKSMIGDGSVDTVLVAFPDNQGRLVGKRVMGQYF
                     LDTVANGSVEACNYLLAVDVEMTPLPGYQAFNWEQGYGDFSAVPDLSTLRLVPWLERT
                     ALVLCDVVEEHDKKTPVSVSPRAILKAQEQRAADLGFLVNIGAELEFFLFKDSYATAA
                     EKRYHGLVPHSDVIEDYHILQTTRDEYIIRDIRNGLQAAGVPVEFSKGEAGKGQHEIN
                     LLYAPATEMADRNTIYKNAAKEIADKHGRSITFMAKYDMDEVGSSCHIHSSLWDLKGK
                     KSQMWDAEKPHNMSDTFRWWLGGLVHASRELALLFAPTVNSYKRFQPDSWAPTAVAWG
                     VDNRTCGLRLVGHGPAYRVESRIPGADVNSYIAFAGVIAAGLHGIENQLDPGDAFVGN
                     AYTDPDVEHIPSTLVEAIDLFEHSDIAKDAFGTDVHHHILNSAK"
BASE COUNT          347 a          763 c          628 g          289 t
ORIGIN      
        1 agaagcccga ggtcgtcgag gcgatcgccg cggccggcgg cgaggccgtc accgtggccg
       61 ccgacgtgcg ggacttcgcc caggtcgagg cggccgtcgc caagaccatc gaagcgttcg
      121 gcggcctgaa cgtgctctac aacaacgccg gcgtcagcct cggcgacgac gacgacgtgg
      181 cgtccacgcc cgagtccacg tgggagctca cgatggacgt caacgtgaag ggcctctggc
      241 tctgctgcaa ggccgccctg cccgccctgc tcgacagcgg caacgcgtcg atcatcaacg
      301 tcgccagctt cgtcgcccac ctcggtgccg ccaccccgca gctcgcctac acgacctcga
      361 agggcgccgt gctgtcgatg acccgcgaga tcgcggtcat ctacgcccgc cagggcatcc
      421 gggccaacgc cctgtgcccc ggcccggtgc tcacgccgct gctcgccaag tacctgtccg
      481 acgaggagaa gcgccagcgc cgcctcgtcc acatcccgat gggccgcttc ggcacctccg
      541 aggagatggt gaacggcgcc ctgttcctcg cctccgacga gtcgagcttc atgaccggcc
      601 agtcgctcct catcgacggc ggcatcaccg ccgcctacac caccccggag tgacggtggg
      661 cggcgcggcc ggcagcggct tctagtctct ccgccacgcc gcactcctgg gagaagcaat
      721 ggaacgggtc acgctcacgc tcgatgcact gaagtcgatg atcggagacg gctcggtcga
      781 caccgtgctc gtcgccttcc cggacaacca gggacggctc gtcggcaagc gggtgatggg
      841 gcagtacttc ctcgacaccg tggcgaacgg gtcggtcgag gcgtgcaact acctgctcgc
      901 ggtcgacgtc gagatgaccc cgctccccgg gtaccaggcg ttcaactggg agcagggcta
      961 cggcgacttc tccgccgtcc ccgacctgtc gaccctgcgg ctcgtcccgt ggctcgagcg
     1021 cacggcgctg gtgctgtgcg acgtggtcga ggagcacgac aagaagacgc cggtctcggt
     1081 ctcgccccgg gcgatcctca aggcgcagga gcagcgggcg gcggacctgg gcttcctcgt
     1141 gaacatcggc gccgagctcg agttcttcct gttcaaggac tcctacgcca ccgcagcgga
     1201 gaagcgctac cacggcctcg tcccgcactc cgacgtgatc gaggactacc acatcctcca
     1261 gaccacccgc gacgagtaca tcatccgcga catccgcaac ggcctccagg ccgcgggcgt
     1321 ccccgtcgag ttctccaagg gcgaggccgg caagggccag cacgagatca acctgctcta
     1381 cgcgcccgcc accgagatgg ccgaccgcaa caccatctac aagaacgcgg ccaaggagat
     1441 cgccgacaag cacggccggt ccatcacgtt catggccaag tacgacatgg acgaggtcgg
     1501 ctcctcctgt cacatccact cgagcctctg ggacctcaag ggcaagaagt cccagatgtg
     1561 ggacgccgag aagccccaca acatgagcga cacgttccgc tggtggctcg gcggcctggt
     1621 ccacgcctcg cgggagctgg cgctgctctt cgccccgacg gtcaactcgt acaagcggtt
     1681 ccagcccgac tcgtgggcgc ccaccgcggt ggcgtggggc gtcgacaacc gcacctgcgg
     1741 cttgcgcctc gtcggccacg ggcccgccta ccgggtcgag tcccggatcc ccggcgccga
     1801 cgtgaacagc tacatcgcct tcgccggcgt catcgcggcg ggcctccacg gcatcgagaa
     1861 ccagctcgac ccgggcgacg ccttcgtggg caacgcctac accgaccccg acgtcgagca
     1921 catcccctcc acgctggtcg aggccatcga cctgttcgag cactccgaca tcgccaagga
     1981 cgccttcggc accgacgtcc accaccacat cctcaactcg gccaagc
//
LOCUS       DATMFS010000152         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_285156, whole genome shotgun sequence.
ACCESSION   DATMFS010000152 DATMFS010000000
VERSION     DATMFS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285156"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VNQ33_02805"
     CDS             <1..1175
                     /locus_tag="VNQ33_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWJ97060.1"
                     /translation="SILVALAALAVVVVGIFTAESRGLGLAIAVALVAGSTYLSRRAL
                     RRDPATLKALPTPGTPVGPAERGVLIVNLKSGGGKAEQLGLEDECRKRGIEPIVLGPD
                     DDLRELAEQAVERGADVLGMAGGDGSQALVASVASAHDLPHVCIPAGTRNHFALDLGL
                     DRDDVIGALDAYGDAVERRIDLAEVNGRVFVNNVSLGVYAKIIQSPGYRDAKGQTAAS
                     MLPELLGPDAEPFDLRFEGPDGVDHAGAQVIQVSNNPYVLSSLAGFGSRARLDTGELG
                     LAAAEIGKAADVAAFVAADAAGRLGSFAGWSEWTAERFTVTSDSPIEAGIDGEALVFD
                     APLEFRALPGAVRVRLPHDAPGYSPAAIEAHSPAWTIGALFRAAAGRPTPVDGQQR"
     gene            complement(1261..>2266)
                     /locus_tag="VNQ33_02810"
     CDS             complement(1261..>2266)
                     /locus_tag="VNQ33_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWJ97061.1"
                     /translation="PTPVSPAADRPPAVAWDWSAALLGLAFAVPAALVARHDVGKGAA
                     FAVGVIPPAVVGLRPLRKGRMASAVLGVLVAVPILAGSILANVPVVAVVGVFLAGIGA
                     ALLAQRSRLGSIALALGLPMIGIGLSYTDVATAAGLAIVMLGGAVYAAAVSLLWPESD
                     PLPPPPHAPPVPTLAYGVLLGATGATAAAIGFALDLDHVGWACAAALLVIRPSAEMQE
                     IRSAGRIASVAIGALLAIGIVRLEPAAGWYGAAIVVVVATVAATRGSRWYITSAFTTF
                     LVFVLLLAGNAAEAASRFDERLSETLLGVGLAGFFGLVIPKLADLDTLQPAPSRPAAK
                     "
BASE COUNT          314 a          872 c          824 g          256 t
ORIGIN      
        1 tgtcgatcct ggtggcgctc gcggccctcg ctgtggtggt cgtcggcatc ttcaccgcgg
       61 agtcgcgcgg cctggggctg gcgatcgcgg tcgccctggt ggcggggtcc acgtacctga
      121 gccgccgagc gctgcggcgc gacccggcca ccctcaaggc gctgcccacg ccgggcacgc
      181 cggtcgggcc cgccgagcgc ggcgtgctga tcgtgaacct gaagtccggc ggtgggaagg
      241 ccgagcagct cggcctggag gacgagtgcc gcaagcgggg catcgagccg atcgtcctgg
      301 gtcccgacga cgacctgcgc gagctggcgg agcaggccgt cgagcggggc gccgacgtgc
      361 tcggcatggc cggcggcgac ggctcccagg ccctggtcgc ctcggtcgcc agcgcccacg
      421 acctcccgca cgtgtgcatc ccggccggca cccgcaacca cttcgccctc gacctgggcc
      481 tcgaccgcga cgacgtcatc ggcgccctcg acgcctacgg cgatgccgtc gagcgacgca
      541 tcgacctcgc cgaggtcaac ggccgcgtct tcgtcaacaa cgtgtcgctc ggcgtctacg
      601 ccaagatcat ccagtcaccc ggctaccgcg acgcgaaggg ccagaccgcg gcgtcgatgc
      661 tgcccgaact gctcggcccc gacgccgagc ccttcgacct ccgcttcgag gggcccgacg
      721 gcgtcgatca cgccggcgcc caggtcatcc aggtgtcgaa caacccctac gtgctctcgt
      781 cgctcgccgg gttcggcagc cgggcccggc tcgacaccgg cgagctcggc ctcgccgccg
      841 ccgagatcgg gaaggccgcc gacgtcgccg cgttcgtcgc cgccgacgcc gccggccgcc
      901 tcggcagctt cgccggctgg agcgagtgga cagccgagcg gttcaccgtg acctcggaca
      961 gcccgatcga agcggggatc gacggcgagg cgctcgtgtt cgacgcaccg ctggagttcc
     1021 gcgccctccc gggcgccgtc cgcgtccgcc tcccccacga cgcccccggc tactcccccg
     1081 ccgccatcga ggcccactcc cccgcctgga ccatcggtgc cctcttccga gccgccgccg
     1141 gccggcccac ccccgtcgac ggccagcagc gctgaggtcc cgtccgcacg ggttcgcatc
     1201 ggaacgtgtc ggtggaccac cgctccaggc tccaggcggc cgaccgacgg ccgggccgcc
     1261 tcacttcgcc gctggtcgcg acggggcggg ttggagggtg tccaggtcgg cgagcttggg
     1321 gatgacgagg ccgaagaagc cggccaggcc gacgccgagc agggtctcgc tgagccgttc
     1381 gtcgaagcgg gaggcggcct cggccgcgtt cccggcgagc aggaggacga agaccaggaa
     1441 cgtcgtgaac gcgctcgtga tgtaccagcg gctgcctcgg gtggccgcca ccgtcgccac
     1501 caccacgacg atggcggcgc cgtaccaacc cgccgccggt tcgagtcgga cgatcccgat
     1561 cgcgaggagc gccccgatgg cgaccgacgc gatgcggccc gcgctgcgga tctcctgcat
     1621 ctcggctgac ggccggatca cgagcagcgc cgccgcgcac gcccagccga cgtggtccaa
     1681 gtcgagggcg aagccgatgg ccgccgccgt cgccccggtc gccccgagca gcaccccgta
     1741 ggccagcgtc ggcacgggcg gggcgtgcgg cggcggaggc agcgggtcgg actcgggcca
     1801 gagcagcgag acggccgcgg cgtagaccgc accgcccagc atgacgatgg ccagcccggc
     1861 ggccgtcgcc acgtcggtgt agctgaggcc gatgccgatc atcggcaggc cgagggccag
     1921 tgcgatgctc cccagccggg accgctgcgc cagcagggcg gcgccgatgc cggcgaggaa
     1981 cacgccgacg acggcgacca ccggcacgtt ggcgaggatc gaaccggcga ggatgggcac
     2041 ggcgacgagc acgccgagca ccgccgacgc catccggccc ttccgcagcg ggcggaggcc
     2101 gacgacggcc ggcgggatga cgccgacggc gaacgccgca cccttcccca cgtcgtgccg
     2161 ggcgacgagc gctgcgggga cggcgaaggc cagcccgagc agcgcggccg accagtccca
     2221 cgcaacagca ggaggccggt cggctgccgg gctcaccggg gtgggc
//
LOCUS       DATMFS010000153         3628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_99385, whole genome shotgun sequence.
ACCESSION   DATMFS010000153 DATMFS010000000
VERSION     DATMFS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99385"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VNQ33_02815"
     CDS             complement(<1..219)
                     /locus_tag="VNQ33_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97062.1"
                     /translation="MDLHALGVTSVFGPCASGSLQARIAPGSFVVPDQLVDRTSGRPD
                     TFYDGEDGHPVHHVAFGDPYDDDLRAVAI"
     gene            208..555
                     /locus_tag="VNQ33_02820"
     CDS             208..555
                     /locus_tag="VNQ33_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97063.1"
                     /translation="MEVHVSGTVVTIQGPRFSTRAESQWFGSHGWEVINMTQHPEAVL
                     CREAGLRYAAIALITDFDAGLEGSGIPAVTQEEVFGFFEANVETVRKVLFRALSAQLP
                     SGRIAAGPGFEVT"
     gene            942..1532
                     /locus_tag="VNQ33_02825"
     CDS             942..1532
                     /locus_tag="VNQ33_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97064.1"
                     /translation="MTLFRRLRQDPGAIRHWLLVGLLAWLLAALVSHALGRADQARER
                     WGRTTVVWVADRALGAGDDLDGALRPARWPAALVPAAAVTDPPADARAAAAVDAGTAI
                     TSAMVERAGAERRTVAVPLPDAHLPVENGDRVDVWATTDPAVTGDGVASTRRVASGAR
                     VTGSSRGSAVLEVAPAQVAAITEAAASATIALVGVP"
     gene            complement(1537..2376)
                     /locus_tag="VNQ33_02830"
     CDS             complement(1537..2376)
                     /locus_tag="VNQ33_02830"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWJ97065.1"
                     /translation="MSYLEAIVLGIVQGLTEFLPISSSGHLRIVPELFGWSDPGASFT
                     AVIQLGTMAAVVIFFWKDLWRIALAWCQDAWRITKTRSTKGERSSDDARMGWYLIVAT
                     IPIAVFGFAFKDQIETGARDLRLIAGTLIIGGLVLLWADKAGERSKGIEHISTQDGVT
                     IGLAQSLALVPGVSRSGATISAGMFLGFTREAAARFSFLLSIPAVVLSGLFSLKDVGD
                     GGIPVGPTAVATVVSFVVGYAAIAWLLRYLTSHTLMVFVVYRVALGLLLFGLLAADVI
                     DAR"
     gene            2535..2801
                     /locus_tag="VNQ33_02835"
     CDS             2535..2801
                     /locus_tag="VNQ33_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97066.1"
                     /translation="MGGTGRAATRSRRLLLALAIVVVIVVSVSAVLALGGAFGSRLDD
                     GGPAPVTVRGTGTATTSTSTSTTAAEPVVLAPPMSIDSTTTEPG"
     gene            complement(2837..>3628)
                     /locus_tag="VNQ33_02840"
     CDS             complement(2837..>3628)
                     /locus_tag="VNQ33_02840"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWJ97067.1"
                     /translation="DDTQTGFGFSVGRTPEDLDISVAVADASRRATRLLGATQPDSGR
                     VTVVLDPYVTAQLLGIIGSTLSGEAVLKGRSLFADRLGDEVAAAGFTLVDDAANPLAY
                     SSSETDGEGLASRRNVLIEGGQLHQFVHNAYTARRAGTVSTANAVRGFSSTPSAGCRA
                     LSLVPGTKLQPELLAEVGDGVLISSVSGLHSGVNPVSGDFSTGAEGLRISGGELGQPL
                     REFTIASSLQRMLAEVAAVGADIEWLPMRSAGVSLVVHGITVSGR"
BASE COUNT          538 a         1302 c         1335 g          453 t
ORIGIN      
        1 gatcgcgacg gcccgcaggt cgtcgtcgta cggatcgccg aaggccacgt ggtggaccgg
       61 gtgcccgtct tcgccgtcgt agaaggtgtc aggtcgtcct gacgtgcggt cgaccagctg
      121 atcgggcacc acgaagctgc cgggggcgat gcgggcctgg agcgatccgc tggcgcacgg
      181 gccgaacacg ctggtgacgc cgagggcgtg gaggtccacg tctcgggcac ggtggtcacg
      241 atccagggac cccggttctc gacccgagcc gagtcccagt ggttcgggtc gcacggctgg
      301 gaggtcatca acatgaccca gcaccccgag gccgtcctgt gccgcgaggc cgggctgcgc
      361 tacgccgcca tcgccctcat caccgacttc gacgccgggc tcgaaggcag cggcatcccc
      421 gccgtcaccc aggaggaggt gttcggcttc ttcgaggcga acgtggagac ggtgcgcaag
      481 gtcctgttcc gggcgctcag cgcccagctc ccctcagggc gcatcgccgc cggtcccggc
      541 ttcgaggtca cctgaccgcc acgaggctcc aacttggagc atcgtggccg ctgagcgccc
      601 aaaccctcca agttggagcc tcgtggcgga agttggaggg ttggtgacgc agagcgacca
      661 cgatcctcca cgatgcaccc acatcaccga cgccgcccgc ccactcgccc gaccacccac
      721 ccgtcagccg acggacgacg gacgacgggc gacgggcgac gggcgacggc gccagatgag
      781 gcgcatcgct cgcagcgggc acgacagcct gcctatggtg cgcgggcctt cccgctgacc
      841 gctcgccgtg gaggtgcgcg cccgtgggtc cggtcccgcc tgctgacatc cgccgtcgtc
      901 gcccgctgcc tggccgccgg gtccacccgc accgcagccg gatgaccctg ttccgccggc
      961 tgcgccagga cccaggggcg atccgccatt ggctgctggt cggcctcctc gcgtggctgc
     1021 tggcagcgct ggtgagccac gctctcgggc gggccgacca ggcccgcgag cggtggggcc
     1081 gcaccaccgt ggtctgggtg gcggaccggg cgctgggcgc cggcgacgac ctcgacggcg
     1141 cgctccgccc ggcgcgatgg cccgccgcgc tcgtgcccgc cgcggcggtc accgacccac
     1201 ccgccgatgc ccgagccgcg gctgccgtcg acgcggggac ggcgatcacc tcagcgatgg
     1261 tcgagcgcgc cggcgccgaa cggcgcactg tcgccgtccc cctccccgac gcccacctcc
     1321 cggtggagaa cggtgaccgg gtcgacgtgt gggccacgac cgaccccgcc gtcaccgggg
     1381 acggggtcgc ttcgacgcgc cgggtcgcga gcggggcgcg ggtgaccgga tcgagccgcg
     1441 gctctgccgt cctcgaggtc gcccctgccc aggtggccgc catcaccgag gccgccgcga
     1501 gcgcgacgat cgccctggtc ggcgtgccgt gacgggtcag cgggcgtcga tgacgtcggc
     1561 ggcgagcagg ccgaacagga gcaggcccag cgccacccgg tacaccacga agaccatcag
     1621 cgtgtgcgac gtgaggtagc gcagcagcca ggcgatggcg gcgtacccga cgacgaacga
     1681 gaccaccgtc gccaccgcgg tggggccgac ggggatcccg ccgtcaccca cgtccttcag
     1741 cgagaacagg cccgagagca cgaccgccgg gatggacagg aggaacgaga agcgagcggc
     1801 ggcctcgcgg gtgaagccga ggaacatgcc agcgctgatg gtggccccgg agcggctcac
     1861 cccggggacg agggcgagcg actgggcgag gccgatggtc acgccgtcct gggtcgagat
     1921 gtgctcgatg cccttgctgc gctcgccggc cttgtcggcc cacagcagca ccagcccgcc
     1981 gatgatcagg gtgccggcga tgagccgcag gtcccgcgcg ccggtctcga tctggtcctt
     2041 gaaggcgaag ccgaagacgg cgatcgggat ggtggcgacg atgaggtacc agcccatgcg
     2101 ggcatcgtcg gacgagcgct cgcccttggt gctccgggtc ttggtgatcc gccaggcgtc
     2161 ctggcaccac gccagcgcga tccgccacag gtccttccag aagaagatga ccaccgccgc
     2221 catggtcccg agctggatga ccgccgtgaa cgacgccccc ggatcgctcc agccgaacag
     2281 ctccggcacg atccgcaggt ggccgctcga cgagatcggc aggaactcgg tgaggccctg
     2341 gacgatcccg aggacgatgg cttcgaggta cgacatcgcc ggacaccttg ccatctcagc
     2401 cgcgacgccc cgcattcacc gcggccctcg gtacggggga tcagccgtcg ccctttcgcc
     2461 ggtggatcga gccgtggttg tctggacggg ttgccaggac gggccggcag caccgcgacg
     2521 cggaggagag ggtcatgggc ggaaccggca gggccgcgac gcggtcgagg cggctgctgc
     2581 tcgctctggc gatcgtggtg gtcatcgtgg tgtcggtgag cgccgtgctc gcgctcggcg
     2641 gggcgttcgg cagccggctc gatgacggtg gacccgcacc ggtcacggtc cgagggaccg
     2701 ggaccgcgac gacctcaacc tcgacctcga ccacggccgc cgaaccagtg gtgctggccc
     2761 caccgatgtc gatcgacagc accaccaccg aaccgggctg agcgcgccgg accggaccga
     2821 cggcacggcg gaccgctcag cggccggaga ccgtgatccc gtggacgacg aggctgacgc
     2881 cggccgagcg catcggcagc cactcgatgt cggcgccgac cgccgccacc tcggccagca
     2941 tgcgctggag cgacgaggcg atggtgaact cgcgcagcgg ctggccgagc tcgccgccgc
     3001 tgatgcgcag gccctcggcc ccggtcgaga agtcgcccga cacggggttg acccccgagt
     3061 gcaggccgga gaccgaactg atgaggacgc cgtcgcccac ctccgcgagc agctcgggct
     3121 ggagcttggt cccgggcacg agcgagagcg cccggcaacc cgccgacggg gtggacgaga
     3181 agccccggac cgcgttggcg gtggagaccg tgccggcacg gcgggccgtg taggcgttgt
     3241 gcacgaactg gtgcagctgg ccgccttcga tcagcacgtt gcggcgcgac gccagtccct
     3301 cgccgtcggt ctccgaggag ctgtaggcca gcgggttggc ggcgtcgtcg acgagcgtga
     3361 acccggccgc ggccacctcg tcgcccagcc ggtcggcgaa cagggaccgg cccttgagca
     3421 cggcctcgcc cgagagcgtc gagccgatga tgccgaggag ctgggcggtc acgtacgggt
     3481 ccaacacgac ggtgacccgg ccggagtcgg gctgggtggc gccgagcagg cgcgtcgccc
     3541 ggcgggacgc atcggccacg gcgaccgaga tgtcgaggtc ctcgggcgtg cggccgacgg
     3601 agaacccgaa cccggtctgg gtgtcatc
//
LOCUS       DATMFS010000154         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_192796, whole genome shotgun sequence.
ACCESSION   DATMFS010000154 DATMFS010000000
VERSION     DATMFS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192796"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VNQ33_02845"
     CDS             complement(<1..782)
                     /locus_tag="VNQ33_02845"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWJ97068.1"
                     /translation="MIDEIRRIQAQAETQIAQAATLEALAEVERSLTGKGSALLDLKK
                     GLGQLDADARRESGLVMNEASEAIRALVAARRAALDTLAQAERAQAERLDLTEVRAGR
                     DAGHLHLITQTIEELEDVFVGLGFTVAEGPEVEDDWHNFTALNFGQYHPARDMQDTFF
                     VGLGGDREVVMRTHTSPVQIRVMEGQEPPIYSVMPGRVYRNEATDATHLAVFHQLECL
                     VVDRGITFAHLAGTIDMFTKAYFGPGWTSRLRPSYFPFTEPSA"
     gene            complement(867..1343)
                     /locus_tag="VNQ33_02850"
     CDS             complement(867..1343)
                     /locus_tag="VNQ33_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ97069.1"
                     /translation="MSNRPRRPPASLRATAAQRAQDAARAAERERTRRQRRWAWIGGV
                     TAAVVLVGAVAAFVVSRPDEQAPPPSEDAVALGCASCHSTNGARSEGPTWKGLYGSTV
                     VLADGSAVTVDEEYLRRAILDPQADVAPGYSMAMPTIDVTPAEADRLVAYIRSLSD"
     gene            complement(1413..>2072)
                     /locus_tag="VNQ33_02855"
     CDS             complement(1413..>2072)
                     /locus_tag="VNQ33_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWJ97070.1"
                     /translation="DVYLDEEAAARPAIAALVERLPDETPVWSLPAGVLDRVGDVTTS
                     QGVLAVVSQRETAWPAPDEAPFVLVLAGLQIPGNVGTLIRAAAAAGAGAVVVAGGVDP
                     TSPKVVRSSAGALFGIDVVVAPSPAEAIGQLRAAGYRIATTVVDGGEPYDAADLGTPL
                     AVVLGSEAHGVDDATRAGADLLVTISMAGPTESLNVAMAGTVLCFEVLRRACSDIDHT
                     A"
BASE COUNT          275 a          740 c          767 g          290 t
ORIGIN      
        1 gccgacggct cggtgaacgg gaagtacgag ggccgcagcc gcgaggtcca gcccggaccg
       61 aagtaggcct tggtgaacat gtcgatggtg ccggcaaggt gggcgaaggt gatgccccgg
      121 tcgacgacca ggcactcgag ctggtggaac acggcgaggt gggtcgcgtc ggtggcctcg
      181 ttgcggtaca cccgccccgg catcacggag tagatgggcg gctcctggcc ctccatcacc
      241 cggatctgca ccggtgaggt gtgggtgcgc atcacgacct cacggtcgcc gccgagcccc
      301 acgaagaacg tgtcctgcat atcccgtgcc gggtggtact gcccgaagtt caaggcggtg
      361 aagttgtgcc agtcgtcttc gacctcggga ccctcggcca ccgtgaagcc cagaccgacg
      421 aacacgtctt cgagctcctc gatggtctgg gtgatcaggt ggaggtgtcc ggcgtcgcgc
      481 ccggcccgca cctcggtgag gtcgagtcgc tccgcctgcg cccgctccgc ctgggcgagg
      541 gtgtcgagcg ccgcacggcg cgccgcgacc agggcgcgga tggcctcgga ggcctcgttc
      601 atgaccaggc cgctctcccg ccgggcgtcg gcgtcgagct ggccgagccc cttcttcagg
      661 tcgagcaggg cggagccctt gccggtgagc gagcgctcca cctcagccag cgcctcgagg
      721 gtcgcagcct gggcgatctg ggtctcggcc tgcgcctgga tgcggcggat ctcgtcgatc
      781 atggtggcgg aaccttgccc gttccgaggg cctcgcgtcc acttggttgc cgcccccatc
      841 cgggtcggtg ggctcccggc gcggggtcag tccgacaggc tccggatgta ggcgacgagg
      901 cggtccgcct cggcaggggt gacgtcgatc gtgggcatcg ccatcgagta ccccggggcg
      961 acgtcggctt ggggatcgag gatcgcccgt cgcaggtact cctcgtcgac ggtgaccgcc
     1021 gagccatccg cgagcacgac cgtgctgccg tagaggccct tccacgtcgg gccttcgctg
     1081 cgggccccgt tcgtgctgtg gcagctcgcg cagccgagcg ccacggcgtc ttcgctcggt
     1141 ggcggcgcct gctcgtcggg cctcgacacc acgaaggcgg ccacggcgcc cacgaggacc
     1201 accgccgctg tgaccccgcc gatccacgcc caccggcgct ggcggcgagt ccgctcccgc
     1261 tcggctgccc tcgccgcatc ctgggcgcgc tgggcggccg tggcgcgaag cgacgccggc
     1321 gggcggcgcg gccggttgct cacggggctc ctcgctggtc catgccacga gtggaagccc
     1381 gcgacgtggc ctctcggcag ggccagaagt ccctaggccg tgtgatcgat gtcgctgcat
     1441 gcccgccgga gcacctcgaa gcacaggacc gtcccggcca tggcgacgtt gagcgactcg
     1501 gtgggaccgg ccatcgagat ggtgaccagg aggtcggcgc cggcccgggt ggcgtcgtcg
     1561 accccgtggg cctcgctgcc gaggaccacg gcgaggggcg taccgaggtc cgcggcgtcg
     1621 tagggctcgc caccgtcgac gacggtggtc gcgatccggt agccggcggc gcggagctgc
     1681 ccgatcgcct cggccggcga gggagcgacg acgacatcga tcccgaacag cgcaccagcc
     1741 gaggaccgca cgaccttggg gctggtgggg tcgaccccac cagccaccac caccgcaccc
     1801 gcgccggcgg ccgccgccgc ccggatcagg gtgccgacgt tgccggggat ctgaagaccg
     1861 gccaggacca gcacgaacgg cgcctcgtcc ggcgcgggcc acgccgtctc gcgctgggac
     1921 acgacggcca gcaccccctg ggacgtggtg acgtcgccga cgcggtcgag cacgccggct
     1981 ggcagggacc agaccggcgt ctcgtcgggc agccgttcga cgagcgcggc gatcgccggc
     2041 cgggcggccg cttcctcgtc gaggtagacg tc
//
LOCUS       DATMFS010000155        10013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_122965, whole genome shotgun sequence.
ACCESSION   DATMFS010000155 DATMFS010000000
VERSION     DATMFS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122965"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2268)
                     /locus_tag="VNQ33_02860"
     CDS             complement(<1..2268)
                     /locus_tag="VNQ33_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97071.1"
                     /translation="MPAAAPRLVNVVPHTHWDREWYKPYPLFRMQLVELLDDLLPRLE
                     GDGSFAHFQLDGQLAVVDDYLEIRPDQGGRITALNESGRLSFGPWYTLPDEFLVSGET
                     HVRNLRLGFATAERFGGSMPVGYLPDMFGHVAQMPQILAGFGLADAVVWRGVPAAIDA
                     PGFWWESPDGTRVRAEYLTDGYGNGARMPTHGKELLEKIDAFRDAQGNKVGDSILWMN
                     GTDHLLPQPHLGRVVAEANEASDDYEIVVTSLAAHIAGMPRDGLPTWKGELRSGARSN
                     LLMGVASCRVDVKQAAARAERWLERVAEPLATCWQPADAYPAAFLAYAWRDVIRNAAH
                     DSICGCSADEVNDAVLHRYAESTRVAEAIAERAVIRVLAEAQQAVAVNPTSRERAGMV
                     TAIIGGDVAPPHTQQLNVRDAVDRTHTINRTTAVPVVQRLIIDDPKISSATVEEQADG
                     TVVATFQADSGPKLQDMTALRLQLEALAEADPEGVVHLDIARTRATQEVLLRTEPVPG
                     FGWRGLAPADLGEHAVRSTGQGLTNGLVTIIPDQGDGTFSLNGTPGMGKIVDDGDEGD
                     TYNWSPPASDGAIERPQDVDVVVSEAGPVRGRIEITRRYRWPTRIDGGRRVGPIDVFV
                     YTTLELRAGEDLVRVAIRFDNPAADHRVRAWFPLPGAASESVAECAFGTVTRGLTAEG
                     GPNEVGLPTYPSRRFVSAGGLTVAHDGLCEYELVDVDGEGDDARASSIAITLLRCVGL
                     ISAGPMTMRALPAGPP"
     gene            2304..2606
                     /locus_tag="VNQ33_02865"
     CDS             2304..2606
                     /locus_tag="VNQ33_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWJ97072.1"
                     /translation="MPKFVMLSTLGPDGHARMRENPERLREVNADVESMGVKVLEQFA
                     LLGPYDFLNILEAPDEGTMARVASTLAARGTLKTLTLTAIDVEDFIDVMRGGPSPA"
     gene            complement(2628..2855)
                     /locus_tag="VNQ33_02870"
     CDS             complement(2628..2855)
                     /locus_tag="VNQ33_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97073.1"
                     /translation="MADAPRDASVRPALEPGTRVEVRSAFDRSWSTGFEVHEVAAGGY
                     RVRRRSDGHVLPVAIPADDVRKERKNSMWWI"
     gene            complement(2918..3403)
                     /locus_tag="VNQ33_02875"
     CDS             complement(2918..3403)
                     /locus_tag="VNQ33_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWJ97074.1"
                     /translation="MSDDATVHTYRTSLAWEGSTGRGYRRYDRTHRLAADPAPADLTL
                     SSDPAFGGDPALLNPEQLLVAAASSCQLLSFLAVAARARLDVVSYRDDAEAVMPEDDD
                     PVRITRIDIRPHVVLADTDAPRPTDERLVHLTEVAHRECFIANSLATEVVVTPTFTWK
                     D"
     gene            complement(3400..3762)
                     /locus_tag="VNQ33_02880"
     CDS             complement(3400..3762)
                     /locus_tag="VNQ33_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97075.1"
                     /translation="MASYRSSRSVLSAFSHRCVLSFASAWSVASAGSCLSVASSGSLL
                     SIGSAGSVLSFGSAGSVLSLGSAGAIGGVGAGPDPGDVVLAARLLAGAALVAAAGQAL
                     LPAPSRSRGTATGTGSIR"
     gene            complement(3886..5931)
                     /locus_tag="VNQ33_02885"
     CDS             complement(3886..5931)
                     /locus_tag="VNQ33_02885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ97076.1"
                     /translation="MPPDPASTQTSDRDPSTAPTEPAAPVRIPHAPGLDGLRGAAVAA
                     VVVFHAGPPTWMPGGFLGVSLFFTLSGYLITTLVLAEVQRDRGMGLAGFWSRRVRRLI
                     PALLMVVLGVAVMARFVKLGRGIRGDIIGALTYTTNWLEIARGQAYGDLFRAPSPLVH
                     LWSLAIEEQFYIVLPLAAWALARRAPDRLRRNLAIGAVGVTVVGFVATVLTNDATVAY
                     YGTIHRAPEIAIGALLACATRPTTEQSPVWLTRVAFGAFAVTVFAWCTTHVSDGWVTG
                     GGLVGFACLSALLVRTASRPGPVSTLLSVSPLRSLGLISYGLYLYHWPIVVLLSPPRV
                     HLAPVPLFVVRVAIALALAIASYLLVEQRFRRGWAATPSGTVIAIGLASLATTLVVTL
                     AIAPTGPKERIVRKPAPAVIDPEDAITQTTTAEAPGPARLAIVGDSVPNWIIRDGGSG
                     LDPKRVALVDGTLEACDGARGNPVARSTTGALVPTPPGCTGWPSQYPRFFERADDVAV
                     LMVGGHAVLDRQIEGQFRGPCDPVAAAWYENDVEERLLFLDQYAEHVVLVLPTWADDL
                     AEWIYPTDHIGRMDCVRSVLSKAAQADGADVIDFADYVCPEGPGRCLPLRTRDGMHID
                     PDKAPAALSWLVDQVLDLTGTGPPKTSKSPASTTSTTPEPDTGSTKGAVAPVPAAGG"
     gene            6060..6767
                     /locus_tag="VNQ33_02890"
     CDS             6060..6767
                     /locus_tag="VNQ33_02890"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWJ97077.1"
                     /translation="MAPQTAPQLRKALKRSAPGLTRERALWADGHEVVVGIDEVGRGA
                     WAGPLTIGAVVLPKQGRVNKIRDSKQLTEREREALFGRIADWCEAWAVGHASEQECDE
                     LGMSEAQRLAARRAIDGLGVTPDQFLVDGKWDFIGGGMTTMIVKGDATCLSISAASIL
                     AKVTRDRMMRTLDESYPAYDFAWNKGYPCPRHKQALRGLGPSAIHRRSWAFMDDIPWT
                     GAVRFMRPDPQGTLFEA"
     gene            complement(6806..7093)
                     /locus_tag="VNQ33_02895"
     CDS             complement(6806..7093)
                     /locus_tag="VNQ33_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97078.1"
                     /translation="MAAPDTTTAHEGADAISDEELERLALAASPDEVPDDAVAWSPTA
                     RDPLIADWYMPAPVAARRDLKTRVITGTVVASIIAINAAGLCVTYGRVILG"
     gene            complement(7235..8488)
                     /locus_tag="VNQ33_02900"
     CDS             complement(7235..8488)
                     /locus_tag="VNQ33_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97079.1"
                     /translation="MTDLTLDEFKAEATAFLDAHAAPKPAATEFVWGQGDDDVALFEE
                     VTPEEEARQLADAKAWLGTKAGAGLHWIAGPTELGGRGLPVAYDHAYRSLETRYAVPN
                     QSFFGIGFGMVVPTILAHGTDETKAYLNRMRSGELVGCQLFSEPGAGSDLAGLTTRAE
                     RDGDEWIISGQKVWTSGAHYSDIGEIICRTDPGLPKHQGLTGFVVDMHAPGVTVRPLR
                     QMTGGAAFNEVFFDGVRVPDDHRLGDVNQGWTVALTTLMNERASIGGGGGGGGMGLAS
                     AARLIQMMEHFGVSDDPVRRDELARLYTGFQVAKFTNQRAAEQLKKTGIPGPELSLGK
                     LMLTDNLTATAEFVAKVLGPRLIADSGEWGTYAWSTFVCGTPGMRIAGGSDETLRNII
                     SERVLGLPKEPGIDARSPFKDLLKN"
     gene            8675..9130
                     /locus_tag="VNQ33_02905"
     CDS             8675..9130
                     /locus_tag="VNQ33_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97080.1"
                     /translation="MDRLRPYPVAVFCAVTLFIWVNRIWLAWTNGDDSVGAKVLWTIP
                     IGAFVLSAVVLAAAMLSGADRGARWFRLLVQAFALGTAGYWMIRVPMITFNQHPDLTA
                     SEEAAFKTVHAVLAVVSVGAAWFAFRWARTAPAGSTSGPPVGPGAVAAG"
     gene            9267..>10013
                     /locus_tag="VNQ33_02910"
     CDS             9267..>10013
                     /locus_tag="VNQ33_02910"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HWJ97081.1"
                     /translation="MGIERIDVRVLLGALGLVAVALSGCSGSSDDHAPEATKTETEAT
                     TTTAVAADDDLKLNEIQVMGTHNSFHIEPPAPELAKLSALDAEQAEQRRYTHPDLTTQ
                     LGEQRIRQIELDVFADSQGGLYSDPAFAASDAEGVAAMDEPGTKVLHEQDVDYHSVCP
                     TLVACLGEVKAWSDANPGHVPVAVNIQFKDGPLIFDVPDQAVPEKWTGEAMDGLDAEI
                     RSVFDEDQIITPDDVRGDHATLEEAVLAKDW"
BASE COUNT         1429 a         3369 c         3875 g         1340 t
ORIGIN      
        1 cgggggaccg gccggcaggg cccgcatggt catggggccg gcagagatga ggccgacgca
       61 gcgcagcaag gtgatggcga tcgaggaggc ccgggcgtcg tcgccctcgc cgtcgacgtc
      121 gaccagctcg tactcgcaca ggccgtcgtg ggcgacggtg agcccgcccg ccgagacgaa
      181 gcggcgggac gggtaggtgg ggaggccgac ctcgttcggg ccgccctccg cggtgaggcc
      241 ccgggtgacg gtgccgaacg cgcactcggc caccgactcc gacgccgcac ccggcagcgg
      301 gaaccaggcg cggacgcggt ggtcggcggc ggggttgtcg aaccggatgg cgacccgcac
      361 caggtcctcc ccggcccgca gctcgagcgt ggtgtacacg aacacgtcga tggggccgac
      421 gcgccggccg ccgtcgatgc gggtgggcca gcggtagcgg cgggtgatct cgatgcggcc
      481 gcggaccgga ccggcctcgg agaccaccac gtcgacgtct tgcggacgct cgatggcgcc
      541 gtcggacgcg ggcggcgacc agttgtaggt gtcgccctcg tcgccgtcgt cgacgatctt
      601 gcccatgccg ggggtgccgt tgaggctgaa ggtcccgtcg ccctggtccg ggatgatcgt
      661 gaccagcccg ttggtgaggc cctggccggt gctgcgcacc gcgtgctcgc cgaggtcggc
      721 gggggcgagg ccgcgccagc cgaagccggg gaccggctcg gtgcgcagca gcacctcctg
      781 ggtggcgcgg gtgcgggcga tgtcgaggtg gacgacgccc tcgggatcgg cctcggcgag
      841 cgcttcgagc tgcagcctca gggccgtcat gtcctggagc ttcgggccgc tgtcggcctg
      901 gaaggtggcg acgacggtgc cgtcggcctg ctcctcgacg gtggcgctgc tgatcttcgg
      961 gtcgtcgatg atgagccgct ggaccaccgg caccgcggtg gtgcggttga tggtgtgggt
     1021 gcggtcgacg gcgtcgcgca cgttgagctg ctgggtgtgg ggtggggcga cgtccccgcc
     1081 gatgatggcg gtgaccatgc cggcgcgctc gcggctggtg gggttgaccg ccaccgcctg
     1141 ctgtgcctcg gcgaggacgc ggatgacggc ccgctcggcg atggcctccg ccacgcgggt
     1201 cgattcggcg tagcggtgga ggacggcgtc gttgacctcg tcggccgagc agccgcagat
     1261 ggagtcgtgg gccgcgttgc ggatgacgtc gcgccaggcg taggcgagga acgccgccgg
     1321 gtaggcgtcg gccggctgcc agcaggtggc gaggggctcg gccacccgct ccagccagcg
     1381 ctcggcccgg gcggccgcct gcttcacgtc gacgcggcac gaggcgacgc ccatgagcag
     1441 gttggatcgg gcgcccgagc gcagctcgcc cttccacgtg ggcaggccgt cgcggggcat
     1501 gccggcgatg tgggccgcga gcgaggtcac gacgatctcg tagtcgtcgg acgcctcgtt
     1561 ggcctcggcc acgactcggc ccaggtgcgg ttggggcagg aggtggtcgg tcccgttcat
     1621 ccagaggatc gagtcgccga ccttgttgcc ctgggcgtcg cggaaggcgt cgatcttctc
     1681 gagcagctcc ttgccgtggg tgggcatgcg ggcgccgttg ccgtagccgt cggtcaggta
     1741 ctcggcccgg acccgggtgc cgtcgggcga ctcccaccag aagccgggag catcgatcgc
     1801 cgccggcacg ccgcgccaca cgacggcgtc ggccagcccg aacccggcga ggatctgggg
     1861 catctgggcc acgtggccga acatgtcagg caggtagccc accggcatcg acccgccgaa
     1921 gcgctcggcg gtggcgaagc cgaggcgcag gttgcggacg tgggtctcgc cggacaccag
     1981 gaactcgtcg ggaagcgtgt accagggccc gaacgagagc cgcccggact cgttcagcgc
     2041 cgtgatccgc ccgccctggt caggacggat ctccaggtag tcgtcgacca cggcgagctg
     2101 cccgtcgagc tggaagtggg cgaacgagcc gtcgccctcg agccgcggga ggaggtcgtc
     2161 gagcagctcg accagctgca tccgaaacag cgggtacggc ttgtaccact cccggtccca
     2221 gtgggtgtgg gggacgacgt tgaccaggcg gggggcggcg gcgggcatgg cccaacgcta
     2281 cggcagggca ctacccttcg cccatgccga agttcgtgat gctctccacg ctgggacccg
     2341 acggccacgc ccggatgcgg gagaaccccg agcggctccg cgaggtcaac gccgacgtgg
     2401 agtccatggg cgtgaaggtg ctcgagcagt tcgcgctgct cggcccctac gacttcttga
     2461 acatcctcga ggcacccgac gaggggacga tggcccgggt ggcctccacg ctcgccgctc
     2521 ggggcacgct caagacgctc acgctcacgg cgatcgacgt ggaggacttc atcgacgtga
     2581 tgcgaggcgg gcccagcccg gcctgaccgc acccgagcgc gggtcggtca gatccaccac
     2641 atggagttct tgcgctcctt gcggacatcg tcggcaggga tggccacggg cagcacgtgg
     2701 ccgtcgctgc ggcggcgcac ccggtagccg ccagccgcga cctcgtggac ctcgaacccc
     2761 gtggaccacg accggtcgaa cgcgctgcgc acctcgacgc gcgtgccggg ctccaacgcc
     2821 gggcgaacgg atgcgtctcg gggggcgtcg gccacccgct gagactaggt ctccgccacg
     2881 cccgctccgg gccaggcggc gccggcgcgg cgagcggtca gtccttccag gtgaaggtgg
     2941 gggtgacgac gacctcggtg gcgagggagt tggcgatgaa gcactcgcgg tgggccacct
     3001 cggtgaggtg gacgaggcgc tcgtcggtgg ggcgcggggc atcggtgtcg gcgaggacga
     3061 cgtggggccg gatgtcgatg cgggtgatgc ggacggggtc gtcgtcttcg ggcatgacgg
     3121 cttcggcgtc gtcgcggtag gacacgacgt cgaggcgggc acgggcggcc acggcgagga
     3181 acgagagcag ctggcacgac gacgccgccg ccaccaggag ctgctcgggg ttgagcaggg
     3241 cggggtcgcc gccgaacgcc ggatccgatg acagcgtgag gtcggcgggc gccgggtcgg
     3301 cggcgaggcg gtgggtccgg tcgtagcgcc ggtagccccg acccgtggat ccctcccagg
     3361 cgagcgaggt ccggtaggtg tggacggtgg cgtcgtcgct caccggatcg accccgtgcc
     3421 cgtggcggtg ccgcgggacc gagacggggc ggggaggagg gcctggccgg cggcggccac
     3481 cagggcggca ccggcgagca ggcgggcggc gagcacgacg tcgccaggat cgggtccggc
     3541 gccgacgccg ccgatggcac cggcggaccc gagcgagagc acggaaccgg cggagccgaa
     3601 cgacaggacc gaaccggccg acccgatgga gagcagcgaa cccgacgacg ccaccgacag
     3661 gcacgagccg gcggacgcga ccgaccaggc tgaggcgaac gacagcacgc aacggtggct
     3721 gaaggcgctc aggacagacc tgctcgaccg gtacgaagcc atgggcgcac ggtaggcgcc
     3781 cgaggccggc cgtgcggctc gtgccctgac cggcagcccc ggcacggtcg tcgccgcacg
     3841 acccggctgg ctggtcggcc gccttcggcg gacgctggtc gggtcctagc cgcctgcggc
     3901 ggggacgggg gcgacggcgc ccttggtgga accggtgtcg ggctccgggg tggtggaggt
     3961 ggtcgaggcg ggggacttcg aggtcttcgg cgggccggtg cccgtgaggt cgaggacctg
     4021 gtcgaccagc cagctcagcg cagccggtgc cttgtccggg tcgatgtgca tgccgtcgcg
     4081 ggtgcgcagc gggaggcacc tccccggacc ctcggggcac acgtagtcgg cgaagtcgat
     4141 gacgtcggca ccatcggcct gggccgcctt gctcagcacc gagcggacgc agtccatgcg
     4201 gccgatgtgg tcggtggggt agatccactc agccaggtcg tcggcccagg tggggagcac
     4261 gagcacgacg tgctcggcgt actggtcgag gaacagcagg cgctcctcga cgtcgttctc
     4321 gtaccacgcg gccgcaacgg gatcgcacgg tccccggaac tgcccttcga tctggcggtc
     4381 cagcacggcg tggccgccga ccatcagcac ggcgacgtcg tcggcccgct cgaagaaccg
     4441 cgggtactgg ctcggccagc ccgtgcagcc cggcggggtg gggaccaggg caccggtggt
     4501 ggagcgggcg accgggttgc cacgagcccc gtcgcacgcc tcgagggtgc cgtcgacgag
     4561 ggccacccgc ttgggatcga ggcccgagcc gccgtctcgg atgatccagt tcggcacgga
     4621 gtccccgacg atggcgaggc gggccggtcc gggcgcctcg gcggtggtgg tctgggtgat
     4681 ggcgtcctcg gggtcgatga cggccggcgc cggcttgcgg acgatcctct ccttgggtcc
     4741 ggtgggagcg atcgcgaggg tcaccacgag ggtggtggcc aagctggcca ggccgatggc
     4801 gatcaccgtg cccgacggcg tcgcagccca gccgcggcgg aagcgctgct cgacgaggag
     4861 gtacgacgcg atggcgaggg ccagggcgat cgccacgcgg acgacgaaca aggggacggg
     4921 ggccaggtgg acccgcggcg ggctcaggag cacgacgatc ggccagtggt acaggtacag
     4981 gccgtagctg atgagcccga ggctccgcag gggcgagacc gaaagcagcg tcgagaccgg
     5041 gccgggccgc gaggccgtgc ggaccagcag cgccgacagg caggcgaacc cgaccagccc
     5101 gccaccggtg acccacccgt cggacacgtg cgtggtgcac caggcgaaca cggtgacggc
     5161 gaaggcgccg aaggccaccc gggtgagcca cacgggggac tgctcggtgg tcggccgggt
     5221 ggcgcaggcg aggagggccc cgatggcgat ctccggggcc cggtggatcg tgccgtagta
     5281 ggcgacggtg gcgtcgttcg tgagcacggt ggccacgaag ccgacgacgg tgaccccgac
     5341 ggcgccgatg gccaggttgc ggcggaggcg gtccggggcc cggcgggcga gggcccacgc
     5401 cgccagcggg agcacgatgt agaactgctc ctcgatcgcc agcgaccaga ggtggacgag
     5461 cggcgacggg gcccggaaca agtcgccgta ggcctggccc cgggcgatct cgagccagtt
     5521 ggtggtgtac gtgagagcgc cgatgatgtc gccccggatg ccccggccca gcttcacgaa
     5581 ccgggccatg acggcgacgc cgagcaccac catcagcagg gccgggatga ggcggcggac
     5641 ccggcgggac cagaagccgg ccaggcccat gccccggtcg cgctggacct cggcgagcac
     5701 gagcgtggtg atcaggtagc ccgacagcgt gaagaacagg gacacgccga ggaagccgcc
     5761 gggcatccac gtgggcggcc cggcgtggaa cacgacgacc gcagccacgg ccgcgccgcg
     5821 cagcccgtcg agcccggggg cgtgcgggat ccgaaccgga gcggccggtt cggtgggggc
     5881 cgtcgacgga tcgcggtccg aggtctgggt ggaggctggg tcgggtggca acggggtggc
     5941 cctcgaaggg cgggacggtc cggccggcgg aggcgaaccg gctcccgatc tttccacgcc
     6001 cgggtggtcg gtacggcacc gtgcgggatc cccggcggcg acggtaggtt cccaccctga
     6061 tggctccgca gaccgctccc cagctccgca aggcgctcaa gcggtcagcg cccggcctga
     6121 ccagggagcg cgccctgtgg gcggacggcc acgaggtggt cgtcggcatc gacgaggtcg
     6181 gccgcggcgc gtgggccgga ccgctcacca tcggtgcggt cgtgctgccg aagcagggcc
     6241 gggtcaacaa gatccgggac tccaagcagc tcaccgagcg tgagcgcgag gccctgttcg
     6301 gccgcatcgc cgactggtgc gaggcgtggg cggtcggcca cgcctcggag caggagtgcg
     6361 atgagctcgg catgtccgaa gcgcagcgcc tcgccgcccg gcgggccatc gatggcctcg
     6421 gcgtcacacc agaccagttc ctcgtcgacg gcaagtggga cttcatcggc ggcgggatga
     6481 ccaccatgat cgtgaagggc gatgccacct gcctgtcgat ctccgcggcg tcgatcctcg
     6541 ccaaggtcac ccgggaccgg atgatgcgca cccttgacga gagctacccg gcctacgact
     6601 tcgcctggaa caagggctac ccctgccccc gccacaagca ggcgctgcgg ggcctcggcc
     6661 cctccgcgat ccaccgccgg agctgggcct tcatggacga catcccctgg accggcgcgg
     6721 tgcgcttcat gagacctgat ccccaaggca cgctcttcga ggcgtagctc ctggccggca
     6781 acgaggacga cgacggcctc gccggtcagc cgaggatcac gcggccgtag gtcacgcaga
     6841 ggccggcggc gttgatggcg atgatcgatg ccaccaccgt gccggtgatc acgcgggtct
     6901 tgaggtcccg tctcgccgcc accggcgcgg gcatgtacca gtcggcgatc agcggatcgc
     6961 gggcggtggg gctccacgca acggcgtcgt cggggacctc gtcgggcgag gcggcgagcg
     7021 ccaggcgctc gagctcctcg tcgctgatcg catcggcgcc ttcgtgcgcc gtggtcgtgt
     7081 cgggagcagc catcgcagcc tcctggtctc ctccgggcca ggctacggga caccgccccc
     7141 gatccgtcgg gctcccgtcc agcgtcgtcg agcaccgcgt cggcgctcgg tggcagggga
     7201 ggtggccgat gcgcccgacg cccgcgcctc cggatcagtt cttgagaagg tccttgaacg
     7261 gtgatctggc gtcgatgccg ggctccttgg ggaggccgag gacgcgctcg ctgatgatgt
     7321 tgcggagggt ctcgtcggac ccgccggcga tgcgcatgcc cggcgtgccg cacacgaacg
     7381 tgctccacgc gtacgtgccc cactcacccg agtcggcgat gagccgcggc ccgaggacct
     7441 tggccacgaa ctcggcggtg gccgtgaggt tgtcggtgag catgagcttg ccgagcgaca
     7501 gctccgggcc ggggatgccg gtcttcttga gctgctcggc ggcgcgctgg ttcgtgaact
     7561 tggcgacctg gaagccggtg tagaggcggg ccagctcgtc gcgccggacc gggtcgtcgg
     7621 agacgccgaa gtgctccatc atctggatca ggcgggccgc actggcgagg cccatgccac
     7681 cgcctccgcc cccgccgccg atggacgccc gctcgttcat gagcgtggtg agggcgacgg
     7741 tccaaccttg gttgacgtcg ccgaggcggt ggtcgtcggg cacgcggacc ccgtcgaaga
     7801 acacctcgtt gaacgcggcg ccgccggtca tctggcgcag gggccgcacg gtcacgccgg
     7861 gggcgtgcat gtcgacgacg aacccggtga ggccctggtg cttgggcagg ccggggtcgg
     7921 tgcggcagat gatctcgccg atgtcggagt agtgggcgcc ggaggtccag accttctggc
     7981 cgctgatgat ccactcgtcg ccgtcgcgct cggcccgggt ggtgaggccg gcgaggtccg
     8041 atccggcgcc gggctcgctg aagagctggc agccgaccag ctcgcccgag cgcatgcggt
     8101 tcaggtacgc cttcgtctcg tcggtgccgt gggcgaggat cgtcggcacg accatgccga
     8161 agccgatgcc gaagaagctc tggttcggca ccgcgtagcg ggtctcgagc gaccggtagg
     8221 cgtggtcgta ggcgacgggc aggccccgtc cgccgagctc ggtgggaccg gcgatccagt
     8281 ggagaccggc gcccgccttg gtgccgagcc acgccttggc atcggccagc tggcgggcct
     8341 cctcctccgg cgtgacctcc tcgaacaggg ccacgtcgtc gtcgccctgg ccccacacga
     8401 actcggtggc ggccggcttg ggcgcggcgt gggcatcgag gaaggcggtg gcctcggctt
     8461 tgaactcgtc gagcgtgaga tcggtcatgg ggtcccctgg cgaagaactt gactgctcgg
     8521 tcaagttacc cggttcccgt cgccgaggcg ggaggcagcg agccgctgcc ctctgcacca
     8581 ggccacccgt gtcggcccgg agcgggagcc ggcgctcgcc ggccggtagg cccgggctgg
     8641 gggacggaga cggtccaccg gtaccttcgg gtgcatggat cggctccgtc cctaccccgt
     8701 cgcggtgttc tgcgcggtga cgctcttcat ctgggtgaac cggatctggc tggcgtggac
     8761 gaacggcgac gactccgtcg gcgccaaggt cctctggacc atcccgatcg gggccttcgt
     8821 cctctcggcg gtcgtgttgg cggcagccat gctgtcggga gccgatcgcg gcgcccgctg
     8881 gttccgcctc ctcgtgcagg ccttcgccct cggtaccgcg gggtactgga tgatccgcgt
     8941 cccgatgatc accttcaacc agcacccgga cctcacggcg tcggaagagg cggccttcaa
     9001 gaccgtgcac gccgtgctgg cggtcgtctc ggtcggcgcc gcctggttcg ccttccgctg
     9061 ggctcggacc gcgccggccg gatcgacctc gggtccgccg gtcggccccg gagccgtcgc
     9121 ggccggctga cctccttcag cgggtggcga cgaccgccag cagcaggtgg tgctcccctc
     9181 cggacacggc cagaggctcc gggtgatcgg cgcaggtcgc gggcgagccg gagcgccgag
     9241 ggcggcggac tggtagtacg gcgcccatgg ggatcgagcg gatcgacgtg cgggtgctgc
     9301 tgggcgccct gggactggtt gccgtggcgc tgtcgggttg cagcgggagc agcgacgacc
     9361 acgcgcccga ggccacgaag acggagacgg aggcgaccac gaccaccgcg gtcgccgccg
     9421 acgacgacct caagctcaac gagatccagg tgatgggaac gcacaacagc ttccacatcg
     9481 aaccgccggc gccggagctg gccaagctga gcgccctcga tgccgagcag gccgagcagc
     9541 ggcggtacac gcaccccgac ctcacgaccc agttgggcga gcagaggatc cgccagatcg
     9601 agctcgacgt gttcgccgac tcccagggtg gcctctactc ggacccggcg ttcgcggcga
     9661 gcgacgccga gggcgtcgcc gccatggacg aaccgggcac caaggtcctc cacgagcaag
     9721 acgtcgacta ccactcggtg tgcccgacgc tcgtcgcctg cctcggcgag gtgaaggcct
     9781 ggtccgacgc caacccgggt cacgtcccgg tggccgtcaa catccagttc aaggacgggc
     9841 cgctgatctt cgacgtaccg gatcaggcgg tgccggagaa gtggacgggc gaggcgatgg
     9901 acggcctcga cgccgagatc cggtcggtgt tcgatgaaga ccagatcatc acgcccgacg
     9961 acgtgcgcgg cgaccacgcc accctcgagg aggccgtgct ggccaaggac tgg
//
LOCUS       DATMFS010000156         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_99523, whole genome shotgun sequence.
ACCESSION   DATMFS010000156 DATMFS010000000
VERSION     DATMFS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99523"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2253
                     /locus_tag="VNQ33_02915"
     CDS             193..2253
                     /locus_tag="VNQ33_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWJ97082.1"
                     /translation="MSVTTIVILVALAVIAYVLLKSIHKIGPAEVGLVNKRVGLRKLK
                     DDNPVAFKGEAGYQATLLMPGLRLRFWPIFGVTKFPWVQIPAGEIGVVIAQVGQPVPI
                     GAKSAEFRPEFGNFSNLEGFLAGGGQKGVQRPVLPPGTLVPLHPVAFMVIGSRQVFGL
                     PVAPELVRMNSGGALTPESFGLDPRQLQVVVIAPSGGQDVVGIVTTLEGQPLESGDIA
                     SRLGGFHDIEAMEQQPAVTDAELIDTLLGSKNDEHNNYQDFQKFLDAGGRIGLQHDPL
                     QYGAYLLNPFLVRVDMVPMLVVNQGEVAVIKGFVGLPTLDTSGSEFKFGSIVRPGHRG
                     IWQEPLRTGKYPINPRVYAAEVVPTFILTLNWSTSNSQAHDLDSRLEPIIGKSREGFV
                     FTIDLQVQIHVSDTRAPKVISMVGTMVNLVNEVLQSAVGNHFRNTLQALEAVQFIETR
                     QAVQRAALEAVTQYLAAYEVETRGVYIQDVVFPEELVEVLTRREIANQERATFVEQQK
                     AETARIETEKAKGTADMQSQLASSAVSVDINRNQAQAREAQANGEAAYVRLTGEAEAD
                     KLRAIGLAEAKATEALGVAKATGYEAQREAIGEMSTALVAVAGAIGEGGIDIMPEVLV
                     TGGGGSFEGLAATLMQALRNGTLTMGSAPRPDGTTPVRRTEVGEALAETAQEVENTEE
                     PLGS"
BASE COUNT          428 a          922 c          873 g          369 t
ORIGIN      
        1 cagccccgtc ggggatcccg tcggaccacg cgtaccagtg gtctccgagc gaccggatgt
       61 gcccggtggt gcgcagcgcc ggggtggcca tggggccgag gatagtcgag aagatcctct
      121 ttcgctcatt cagacgtctt ggtacgctca cctcgactcg acatcgtgcc gagtggtgga
      181 aagtgagttc ccatgtccgt aacgaccatc gtgatcctcg tcgccttggc ggtgatcgcg
      241 tacgtgctgc tcaagtcgat ccacaagatc ggcccggcag aggtgggtct ggtcaacaag
      301 agggtcgggc tgcgcaagct gaaggacgac aacccggtcg cgttcaaggg cgaggccggc
      361 taccaggcca cgctcctcat gcctgggctc cggctccggt tctggccgat cttcggggtc
      421 accaagttcc cgtgggtgca gatcccggcc ggtgagatcg gcgtcgtcat cgcccaggtc
      481 ggccagccgg tgccgatcgg cgccaagtcc gccgagttcc ggcccgagtt cggcaacttc
      541 tcgaacctgg agggcttcct cgccggcggc ggccagaagg gcgtgcagcg cccagtcctg
      601 cctcccggca ccctggtgcc gctgcacccg gtcgcgttca tggtcatcgg cagccggcag
      661 gtgttcggtc tcccggtcgc acccgagctg gtgcggatga acagcggtgg ggcgctcacc
      721 cccgagtcgt tcgggctcga cccccggcag ctccaggtgg tggtcatcgc ccccagcggc
      781 ggccaggacg tggtcggcat cgtcaccacg ctcgagggcc agcccctcga gtcgggcgac
      841 atcgccagcc gcctcggcgg gttccacgac atcgaggcga tggagcagca gcccgccgtc
      901 accgacgccg agctgatcga cacgctgctc ggctccaaga acgacgagca caacaactac
      961 caggacttcc agaagttcct cgacgccggc ggccgcatcg gcctccagca cgacccgctc
     1021 cagtacggcg cctacctgct gaacccgttc ctggtccgcg tcgacatggt gccgatgctc
     1081 gtggtgaacc agggcgaggt cgccgtcatc aagggcttcg tcggcctgcc cacgctcgac
     1141 acgtcgggct cggagttcaa gttcggctcg atcgtgcgcc ccgggcaccg gggcatctgg
     1201 caggagccgc tgcgcaccgg caagtacccc atcaaccccc gcgtgtacgc ggccgaggtc
     1261 gtgccgacgt tcatcctgac gctcaactgg tcgacgtcga actcccaggc ccacgacctg
     1321 gactcccggc tggagccgat catcggcaag agccgtgagg gcttcgtctt caccatcgac
     1381 ctgcaggtgc agatccacgt gtccgacacc cgggcgccga aggtgatctc gatggtcggc
     1441 accatggtca acctggtcaa cgaggtgctg cagtcggcgg tgggcaacca cttccgcaac
     1501 accctccagg ccctcgaagc cgtgcagttc atcgagaccc gccaggccgt gcagcgcgcc
     1561 gcgctcgaag cggtgacgca gtacctggcg gcctacgagg tggagacccg cggcgtctac
     1621 atccaagacg tggtgtttcc cgaggagctc gtcgaggtcc tgacccggcg ggagatcgcc
     1681 aaccaggagc gggccacgtt cgtcgagcag cagaaggcgg agacggcccg catcgagacc
     1741 gagaaggcca agggcaccgc cgacatgcag tcccagctgg ccagctcggc cgtgtcggtc
     1801 gacatcaacc gcaaccaggc ccaagcccgc gaggcgcagg ccaacggcga agccgcctac
     1861 gtgcgcctca ccggtgaggc cgaggccgac aagctccgcg ccatcggcct cgccgaggcc
     1921 aaggccaccg aggcgctcgg cgtcgccaag gccaccggct acgaggccca gcgcgaggcc
     1981 atcggcgaga tgtcgacggc cctggtggcc gtcgccggcg ccatcggcga aggcggcatc
     2041 gacatcatgc cggaggtcct cgtgaccggc ggcggcgggt ccttcgaggg cctggccgcc
     2101 accttgatgc aggcgctgcg caacggcacc ctcaccatgg gctccgcgcc ccgccccgac
     2161 ggcaccaccc cggtgcgccg caccgaggtc ggcgaggccc tggccgagac cgcccaggag
     2221 gtcgagaaca ccgaggagcc cctcgggagc tgaaccggct cctcaccgac cgactcgctc
     2281 ggaccaggcc accggccccc tccccgggag ggggccggtg cgcgtcccgg gtcgaagcca
     2341 cccgccggca aggcatcgcc cgcgagggtt gccggcggag caggaggcga agaagttgga
     2401 ggatctcggt cgctcagcga caccctccct ccaagttccg ccaaggacca ccaacttgga
     2461 cgatccgggt caaagagcgc catctgcctc caagttggag gatcctggtc ggtcaggggc
     2521 cggcggcgac ctgggcggcg agcttgccga tgagctcgcc catgccgggg aggaggtgct
     2581 ggaacacgct gg
//
LOCUS       DATMFS010000157         2093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_76036, whole genome shotgun sequence.
ACCESSION   DATMFS010000157 DATMFS010000000
VERSION     DATMFS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76036"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VNQ33_02920"
     CDS             <1..643
                     /locus_tag="VNQ33_02920"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HWJ97083.1"
                     /translation="ATGGGLARWAAAHPDQAQVTIAGQAGCGFVQGGTRVFPQGDQEI
                     SPECDRYVDETLAAKVAELHPDVVMMMTTAWDVIDQRFEGTGPEAPTDPEYRQHVQQD
                     FADATQAVLGAGAPQVVWIAEPTVNPLWNPVHSPQEEPERHAVLHDAMADLAVQHPDQ
                     VTVADLAWWIDDAGLAEDHPTRPDGVHLSTPAAESLATQWLGPLLVNAAVRGR"
     gene            complement(630..1610)
                     /locus_tag="VNQ33_02925"
     CDS             complement(630..1610)
                     /locus_tag="VNQ33_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HWJ97084.1"
                     /translation="MRAIVLTEYGDPSVLTLTDIAAPEIGPEDVLVEVAATALNRADL
                     LQRRGFYPEPGPKREHEVPGMELSGRVVAVGARVARWAVGDEVMAIVAGGAYAELAAV
                     HERQLMPVPESVDVADAAAIPEVGITAHDALVVQGGLRSGGWALVHAGASGVGSMAVQ
                     IVKAMGAHVVATTSAGKVEAVRALGADRVVDYGAEDYVEAVSEATKGIGVDTVLDVVG
                     GDYLMRNVAAIRVGGSIVQVGVMGGGATEINLGMLLPKRAHLMGTVLRSRPLEEKAAA
                     SARFTAEVVPAIESGAVRPVIDRRYPLADIAEAHRYLETNANVGKVLIDL"
     gene            1634..>2093
                     /locus_tag="VNQ33_02930"
     CDS             1634..>2093
                     /locus_tag="VNQ33_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97085.1"
                     /translation="MDFALSADLVALGQEARAVGRAGTDRREIREDGWLMGYDPGFAL
                     ELAERGWLGMTWPTEVGGGGRRAIDRFVVFEALIAEGAPLAACYFADRQIGPTLLQFG
                     TSEQQARWLPGILAGADVWCIGMSEPDAGSDVASLRTRAVRAGDGSGDWIV"
BASE COUNT          279 a          805 c          737 g          272 t
ORIGIN      
        1 ggccaccggc ggcgggctcg cccgctgggc cgctgcccat cccgaccagg cccaggtgac
       61 catcgccggc caggccggct gcgggttcgt gcagggcggc acccgggtgt tcccccaggg
      121 cgaccaggag atctcgccgg agtgcgaccg gtacgtcgac gagacgctgg ccgccaaggt
      181 cgccgagctc catcccgacg tcgtgatgat gatgaccacg gcgtgggacg tcatcgacca
      241 gcggttcgag ggcaccggcc ccgaggcccc gacggacccg gagtaccgcc agcacgtgca
      301 gcaggacttc gccgacgcca cccaggccgt cctcggcgcc ggtgcgcccc aggtggtgtg
      361 gatcgccgag cccaccgtga acccgctctg gaacccggtg cactcgcccc aggaggagcc
      421 cgagcgccat gccgtgctcc acgacgccat ggccgatctc gccgtccagc accccgatca
      481 ggtcacggtg gccgacctgg cctggtggat cgacgacgcc ggtctggccg aggaccaccc
      541 gacccggccc gacggcgtcc acctcagcac gcccgcggcg gagtcgctgg cgacccagtg
      601 gctgggaccg ctcctggtca acgccgccgt cagaggtcga tgagcacctt gccgacgttg
      661 gcgttggtct cgaggtaccg gtgggcctcg gcgatgtcgg ccagcgggta gcgccggtcg
      721 atcaccggcc ggaccgcgcc ggactcgatg gcggggacca cctcggcggt gaaccgggcc
      781 gatgccgccg ccttctcctc caggggccgg gaccgcagca cggtccccat gaggtgcgcc
      841 cgcttcggca gcagcatccc gaggttgatc tcggtggcac cgccgcccat cacgccgacc
      901 tggacgatcg agccgcccac ccggatggcc gccacgttgc gcatcaggta gtcgccgccc
      961 accacgtcga gcacggtgtc gacgccgatc cccttcgtgg cctccgacac ggcctccacg
     1021 tagtcctcgg cgccgtagtc gaccacccgg tcggcaccca gcgcccggac cgcctcgacc
     1081 ttgccggcgg aggtcgtggc cacgacgtgg gcgcccatgg ccttcacgat ctggaccgcc
     1141 atcgacccca caccggacgc tccggcgtgg acgagcgccc acccgccgga gcggagcccg
     1201 ccctggacga ccagggcatc gtgcgcggtg atcccgacct cggggatcgc cgccgcatca
     1261 gccacgtcca cgctctcggg caccggcatc agctggcgct cgtgcacggc ggccagctcg
     1321 gcgtaggcac cgccggccac gatcgccatc acctcgtcgc cgaccgccca ccgggcgacg
     1381 cgggccccga ccgcgacgac ccggcccgac agctccatgc ccggcacctc gtgctcgcgc
     1441 ttcgggccgg gctccgggta gaacccgcgg cgctgcagca ggtccgcacg gttcaaggcg
     1501 gtggccgcca cctccaccag cacgtcttcc ggtccgatct ccggggccgc gatgtcggtc
     1561 agcgtgagca cggacggatc tccgtactcg gtcaagacga tggctcgcat gcgcgcaccc
     1621 tacggtcctg gtcgtggact tcgcgctctc ggccgacctc gtcgccctcg ggcaggaggc
     1681 gcgtgccgtg gggcgggccg gaaccgaccg gcgcgagatc cgggaggacg gctggctcat
     1741 ggggtacgac cccggcttcg ccctcgagct ggccgagcgg ggctggctgg gcatgacgtg
     1801 gccgaccgag gtgggcggcg gcgggcggcg ggcgatcgac cgcttcgtgg tgttcgaggc
     1861 gctgatcgcc gagggggcgc ccctggccgc ctgctacttc gccgaccggc agatcggccc
     1921 gacgctcctc cagttcggca cctcggagca gcaggcgcgg tggctgcccg ggatcctcgc
     1981 cggcgccgac gtgtggtgca tcgggatgag cgaacccgat gccggttccg acgtcgcctc
     2041 gctgcgcacc cgagccgtcc gggccggcga cggatcgggc gactggatcg tcg
//
LOCUS       DATMFS010000158         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_29910, whole genome shotgun sequence.
ACCESSION   DATMFS010000158 DATMFS010000000
VERSION     DATMFS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29910"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..609
                     /locus_tag="VNQ33_02935"
     CDS             151..609
                     /locus_tag="VNQ33_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWJ97086.1"
                     /translation="MRRLRQLLRSSTVSIVATTTSLVVLGALVTTQAVSPGWANLAAT
                     AVGTVPTFELNRRWVWHRQDAASTKREVIPFAALSVAGMALSTAAVSLAGRWSDHAGL
                     TGAGQALAVQGADVAAFGTRWLAQFVILDRIVFADHARVGQDRRVPVVSR"
     gene            614..>2017
                     /locus_tag="VNQ33_02940"
     CDS             614..>2017
                     /locus_tag="VNQ33_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ97087.1"
                     /translation="MTLRTRLVVALVALSTVGLAVFGVSTYTLYQRSLAKQLDQDLAA
                     NSLGQAGRLARDAVQGAFDPSSCTSDSSTTTTEDVVVPPDLDGGTSVPGGALDAYAEL
                     RSSDGNVLACAVPVSSDSRPDLPDDLHVEPGQMEYLTVRSTEGSGTWRVLARVADSGG
                     PATTQFRSPDGDLVDRTQTLGALEGATVVTAVRTAGLEESLARLVRIELVAAVLLLTA
                     LGGGAWFILRRGLRPLEEMADSAATITAGDLSERVEPADGRTEVGQLGLALNTMLDGI
                     EDSFREREATEQRLRQFLADASHELRTPLTSIQGFAELFRLAAQGEPNDLERLDLPVV
                     LARIEQDADRMKALVEDLLLLARLDAPKAVDPVALDLAVLAAEACSTAAALQTDRTIS
                     LEAPEPVPVRGVGDHLHRAIVNLVANAVKHTPPGTPIEVTVAANATTRHASVTVRDHG
                     PGLDDDALAHVFDRFWQA"
BASE COUNT          249 a          802 c          684 g          282 t
ORIGIN      
        1 gctcgcctcc tgcctcgccg ccctggcggt ggcagcggcg gccggcgtca cctcgaccct
       61 cggcctgctc gtgctcctga cggcagcgaa cgtcctcgcc accgccgtcc gcttcgtcgg
      121 cctgcggcgt tcgatgggac gctgaccgag atgcgccgcc tgcggcagct gctgcgctcc
      181 agcaccgtgt cgatcgtcgc caccaccacg agcctcgtcg tgctgggcgc gctggtgacg
      241 acgcaggcgg tgtcgccggg ctgggcgaac ctcgccgcca ccgccgtggg caccgtgccg
      301 accttcgagc tgaaccgccg ctgggtctgg caccgccagg acgccgcgtc caccaagcgg
      361 gaggtgatcc cgttcgcggc gctgtcggtc gccgggatgg cgctttcgac cgcggccgtc
      421 agcctcgccg gccgctggag cgaccacgcc ggcctcaccg gcgccggcca ggccctggcc
      481 gtccagggcg ccgacgtcgc cgccttcggc acgcgctggc tcgcccagtt cgtgatcctc
      541 gaccgcatcg tgttcgccga ccacgcccgc gtcgggcagg atcggcgagt ccccgtcgtc
      601 tcccggtgat cgcatgaccc ttcgcacacg gctggtggtg gcgctcgtgg cgctgtcgac
      661 ggtggggctg gcggtgttcg gcgtctcgac ctacaccctc taccagcgct cgctcgcgaa
      721 gcagctcgac caggatctgg cggccaactc gctcggccaa gccggccgcc tcgcccgcga
      781 cgcggtccag ggcgccttcg atccctcgag ctgcacctcc gacagcagca ccaccaccac
      841 ggaggatgtc gtcgtgcctc cggacctcga cgggggcacc agcgtgcccg gtggcgccct
      901 cgacgcctac gccgagctgc ggtcgagcga cgggaacgtc ctcgcgtgcg ccgtcccggt
      961 gtcgtctgac agccgccccg acctccccga tgacctccac gtcgagccgg gccagatgga
     1021 gtacctgacc gttcgttcga ccgagggctc gggcacctgg cgcgtcctcg cgcgggtcgc
     1081 ggactccggc ggtccggcga cgacgcagtt ccggagcccc gacggcgacc tggtggaccg
     1141 gacccagacg ctcggtgcgc tcgagggggc caccgtcgtc accgccgtgc gcaccgccgg
     1201 cttggaggag tccctcgccc gcctcgtccg catcgagctg gtcgcggcgg tcctcctgct
     1261 caccgcgctg ggcggcggtg cctggttcat cctccggcgc ggcttgcgcc cgctcgagga
     1321 gatggcggac tcggccgcca ccatcacggc aggtgacctg tcggagcgcg tggagccggc
     1381 cgacggccgg accgaggtcg gccagctggg cttggccttg aacacgatgc tcgacggcat
     1441 cgaggactcg ttccgcgagc gcgaggccac cgagcagcgg ctccgccagt tcctcgccga
     1501 cgcatcgcac gagctgcgca ccccgctgac ctcgatccag ggcttcgccg agctgttccg
     1561 cctggccgcc caaggcgaac cgaacgacct ggagcggctc gacctcccgg tcgtgctcgc
     1621 ccgcatcgag caggacgccg accgcatgaa ggcgctggtc gaggacctgc tgctgctcgc
     1681 ccggctcgac gcccccaagg ccgtcgaccc cgtcgcgctc gacctcgccg tcttggcggc
     1741 cgaggcctgc agcacggccg ccgccctgca gaccgaccgg acgatctcgc tcgaagcgcc
     1801 cgagccggtg ccggttcgag gcgtcggcga ccacctccac cgagccatcg tcaacctggt
     1861 cgccaacgcg gtgaagcaca cgccgccggg aacgccgatc gaggtcacgg tggccgccaa
     1921 cgcgaccacc cgccacgcga gcgtcacggt ccgcgaccac gggcccggcc tcgacgacga
     1981 cgcgctcgcc cacgtgttcg accgcttctg gcaggcc
//
LOCUS       DATMFS010000159         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_308795, whole genome shotgun sequence.
ACCESSION   DATMFS010000159 DATMFS010000000
VERSION     DATMFS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308795"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..747
                     /locus_tag="VNQ33_02945"
     CDS             379..747
                     /locus_tag="VNQ33_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWJ97088.1"
                     /translation="MLKHLQSKAEWNEKSLGAKGFTLIELLVVIVILGILAAVVVFSV
                     RGITDKGDASACEAQLSTVETAVEAYRAQYDSYPPDMSNLTTSFLKKAPSDKWGTISV
                     NSSNGTVTLTNPNGNKCPTP"
     gene            complement(971..2080)
                     /locus_tag="VNQ33_02950"
     CDS             complement(971..2080)
                     /locus_tag="VNQ33_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWJ97089.1"
                     /translation="MHIDQVNVRPIEGLVRELWDRGGTDIHIAAGAPPMFRIDGELHP
                     VAGHAPLQPDEIETLVGAMLPQELQDAFLANKVVDFSFSWEDLTRFRGNVFRQRGQIG
                     IALRAIAYEIPTMEDLLLPPVLGQFCLQRQGLVLMTGPTGAGKSTTQAAMIGRINGTQ
                     RRHILTLEDPIEYVHQNRTSIVSQREIGFDTLSFQDGLKSALREDPDVVLVGEMRDPE
                     SIAATLTVAETGHLVFATLHTNDASSALDRIVDVFPSERQAQVRVQLASSLTGVVAQR
                     LIPRTDGGMIAAFEVLLANTATRSLIREGKSHQIRNVIAQSVREGMQTLEQSLSVLVA
                     HGLITYEDALAHAVVPKEVRPPAPVAAPAPMAPTA"
     gene            2203..3759
                     /locus_tag="VNQ33_02955"
     CDS             2203..3759
                     /locus_tag="VNQ33_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWJ97090.1"
                     /translation="MLTSQARRFGDALVDRHVVTRELVESALDEASRTGQAFPEVIAA
                     QAEIPPSDLAAAWAAALGAHHIDFDTVVVEPEAVALLPEQLGRAARAVPVSVQGRTLV
                     VAFATPLDPAMVKQVTDHVRGNGGDIEIGLSDGGAIDAALNAAYGSSPTTPEATVGHA
                     EPSAELYRIFDRALELGASDIHLAANQPPFIRVVGELVRMPDEPALSAAKVRELAYSI
                     LSVRQQKTFEEVSELDTSHAMGSRVRFRVNVFVQRNAVGVAFRVIPFEVIDFDQLGLP
                     PVVRTFANYPRGLILVTGPTGSGKSTTLASLIDIVNRERQSHIITIEDPIEFVHHSKV
                     ALVNQREVGSDTAGFGAALTSAMRQDPDVILVGEMRDLETISTAITAAETGHLVYATL
                     HTQDAAQTVDRIIDVFPAEQQAQVRIQLANSLMGVVTQQLVPTADGRARAVASEVMVA
                     NHPIRALIRDGKVHQIHNAMMSGRKIGMQSMDDSLADLVRRGLVTLDQAQKRAQNPEE
                     LSRLIGTAPS"
BASE COUNT          607 a         1319 c         1289 g          572 t
ORIGIN      
        1 cagtcggtcg tcgacaagaa gctcgtgtgc ggcgccggcg ggaccgccaa ggtcatcggc
       61 cgggcgcgcg tgcgcttcga gaacgactac gacaactccg tgggggccga caagttcaag
      121 cgcgccgtga tcccgaaggt cctgtcgtgg cagatccgca gctagctcgc ccgggctgac
      181 gtggatcgcc gggtggccgg gtcgcacctc atcggcagca gatcacgagg agtgatcctt
      241 acgacccaca gacatcggtg aaagccgtca cctatactca ccaacgccgt ggggggcacc
      301 cctccaaggc cgaggtggta ccggcatcgc aaatccgcgg tgtcagaccg aacaaccatc
      361 ccttgggggg aaatcaacgt gctcaagcat ctgcagtcga aggccgagtg gaacgagaag
      421 tccctcggag cgaagggctt cacgctcatc gagctcctgg tcgtcatcgt gatcctcggc
      481 atcctcgccg ccgtggtcgt gttctccgtc cgtggcatca ccgacaaggg cgacgccagc
      541 gcctgcgagg ctcagctcag caccgtcgag accgcggtcg aggcctaccg ggcgcagtac
      601 gacagctacc cgccggacat gagcaacctc accaccagct tcctcaagaa ggcgccgagc
      661 gacaagtggg gcaccatctc ggtcaacagc tccaacggca cggtcaccct caccaacccg
      721 aacggtaaca agtgcccgac gccctgatcc gtggcctcgg cgtgagccga acctgatcta
      781 cggcgagccc gctccttcgg gagcgggctc gtccgcgtcg gggtccaccg gtcaggacct
      841 ggccggagtg ggaccggacc gggtgacagg cccggtccca ctcgtctcca cgcgatcgag
      901 cccgccctca atagggcggg ctcgatcatg ttgctcagtg ccgggcagtg ccggcgatgc
      961 gtgcctgcgg tcaggccgtg ggggccatcg gcgccggggc ggccaccgga gcgggcggcc
     1021 gcacctcctt gggcacgacg gcgtgggcga gggcgtcctc gtaggtgatc aggccgtggg
     1081 ccacgagcac cgacagcgac tgctccaggg tctgcatgcc ctcccggacg ctctgggcga
     1141 tgacgttgcg gatctggtgg ctcttgccct cccggatgag cgagcgggtc gccgtgttgg
     1201 ccagcaggac ctcgaaggct gcgatcatcc cgccgtcggt gcgggggatc agccgctggg
     1261 cgacgacacc ggtgagcgaa gaggccagct ggactcgcac ctgtgcctgg cgctccgacg
     1321 ggaacacgtc gacgatgcgg tcgagggcgc tcgaagcatc gttcgtgtgg agcgtggcga
     1381 acaccaggtg accggtctcg gccacggtga gggtggcggc gatgctctcg gggtcgcgca
     1441 tctcgcccac gagcacgaca tcggggtcct cgcgcagggc ggacttgagg ccgtcctgga
     1501 agctcagggt gtcgaagccg atctcgcgct gggacacgat cgacgtccgg ttctggtgga
     1561 cgtactcgat cggatcctcg agcgtgagga tgtggcgccg ctgcgtgccg ttgatgcggc
     1621 cgatcatggc cgcctgggtc gtgctcttgc cggcgccggt gggcccggtc atgagcacca
     1681 ggccctggcg ctggaggcag aactgaccga gcacgggcgg gaggaggagg tcctccatcg
     1741 tcgggatctc gtaggcgatc gcccgcaggg cgatgccgat ctgcccgcgc tggcggaaga
     1801 cgttgccgcg gaagcgggtg aggtcctccc agctgaacga gaagtcgacg accttgttgg
     1861 ccaggaacgc gtcctggagc tcctggggga gcatggcgcc caccagggtc tcgatctcgt
     1921 cgggctggag cggggcatgg ccggcgaccg ggtggagctc gccgtcgatc cggaacatcg
     1981 gcggcgcacc agccgcgatg tggatgtcgg ttcccccccg gtcccagagc tctcgcacca
     2041 gcccctcgat ggggcggacg ttgacctggt cgatgtgcat gggacaagtc tcgcacgggt
     2101 cagacggcgc tgctactgaa ggagcggtcc actagggtcc tctggtcctc gcccctcgaa
     2161 gcgggcggga cgcggacgtc catccggggg ggatacagca ccatgctcac cagtcaggct
     2221 cgtcgcttcg gggacgcctt ggtcgatcgc cacgtcgtga cgcgcgagct cgtcgagtcc
     2281 gcactcgacg aggcgtctcg caccgggcag gccttcccgg aggtcatcgc ggcacaggcg
     2341 gagatcccgc catcggacct tgcggcggcg tgggccgcgg cgctcggcgc ccaccacatc
     2401 gacttcgaca ccgtggtcgt cgagcccgag gcggtggcgc tgctacccga gcagctcggc
     2461 cgggccgcac gggccgtgcc cgtgtcggtc cagggccgga cgctcgtcgt cgccttcgcc
     2521 acccccctcg acccggcgat ggtcaagcag gtcaccgatc acgtgcgcgg caacgggggc
     2581 gacatcgaga tcggcctctc cgacggtggc gcgatcgatg ccgccctcaa cgccgcgtac
     2641 ggctcgagcc cgacgacgcc tgaggcgacc gtcggccatg ccgagccctc ggctgagctg
     2701 taccgcatct tcgaccgcgc cctcgagctc ggtgcatcgg acatccacct ggccgcgaac
     2761 cagcccccgt tcatccgcgt ggtgggcgag ctcgtgcgga tgcccgacga gccggcgctc
     2821 agcgccgcca aggtgcgcga gctggcctac tcgatcctgt cggtccgcca gcagaagacc
     2881 ttcgaagagg tgtccgagct cgacacctcc catgccatgg ggagccgggt ccggttccgc
     2941 gtcaacgtct tcgtccagcg caacgcggtc ggcgtcgcct tccgcgtgat ccccttcgag
     3001 gtcatcgact tcgaccagct cggcctgccg ccggtggtcc ggaccttcgc caactacccg
     3061 cgtggcctga tcctcgtgac cgggccgact ggctcgggca agtcgacgac gctcgcgtcg
     3121 ctcatcgaca tcgtgaaccg cgagcggcag tcgcacatca tcacgatcga agaccccatc
     3181 gagttcgtcc accactcgaa ggtcgcgctc gtgaaccagc gcgaggtcgg ctcggacacg
     3241 gcgggcttcg gcgccgcgct cacgtcagcc atgcggcagg accccgatgt catcctcgtc
     3301 ggcgagatgc gcgacctcga gacgatctcc accgccatca ccgcggcgga gaccggtcac
     3361 ctcgtctacg ccaccttgca cacgcaagat gccgctcaga cggtcgaccg catcatcgac
     3421 gtgttccctg cggagcagca ggcccaggtc cggatccagc tcgccaactc gctcatgggc
     3481 gtcgtcaccc agcagctcgt gcccaccgcg gacggccggg cgcgtgccgt cgcctcggag
     3541 gtgatggtgg cgaaccaccc catccgggcc ctcatccgcg acggcaaggt ccaccagatc
     3601 cacaacgcga tgatgtcggg ccgcaagatc gggatgcaga gcatggacga ctcgctcgcc
     3661 gacctcgtgc gccgcggtct cgtgacgctc gaccaagcgc agaagcgggc ccagaacccc
     3721 gaggaactga gtcgtctcat tggtaccgcc cccagctgac gcccccgagg cgacagcggc
     3781 tccgacg
//
LOCUS       DATMFS010000160         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_7081, whole genome shotgun sequence.
ACCESSION   DATMFS010000160 DATMFS010000000
VERSION     DATMFS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7081"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VNQ33_02960"
     CDS             complement(<1..955)
                     /locus_tag="VNQ33_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97091.1"
                     /translation="MNLLVLCPHFVPDVAPTGEVMTQIAKGLTDRGNRLHIVTSLPWY
                     QHHALEPGWEGQIVRHEDTEWGRITRVHPFPTDKRNIPARALAFAGFTALSGLAGVVT
                     RSEPDAVLAMSPPLTLGAAGWAIAKAHRVPFVFNIQDVFPDVAVELGAITDPRVIEVA
                     SWLERETYLRADAVTVLSEDLKANVAAKISGRRGAAGDPGKVRVIPNFIDTDFIRPAE
                     RENAYRREFGLEGKTVVMYAGNVGWSQSLDLVLAAAAHLADDPDVVFVINGGGVAREG
                     LRAQAAGLANVRFVDMQPKARLPEVAAAADIHVVPLRTGLAR"
     gene            complement(952..2139)
                     /locus_tag="VNQ33_02965"
     CDS             complement(952..2139)
                     /locus_tag="VNQ33_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWJ97092.1"
                     /translation="MTALSATRTTPLRVGVNLLWLVPGVVGGSEEYTTRLLRGLAADL
                     PPDLHLTLFALRSFADAHPDLVAAYPTVTARQDGHRKAERVATEATWLAREAGRRNIE
                     LLHHAGGVVPPGPAIGRIPAALTIHDLQPLVMPENFTSVKRRWLATMLPRSARRASMV
                     FTPSDPASASVVERLAVPEARVRTVPHGIEPPQPVPEGDLAAVRERYRLGEQVVLYPA
                     ITYPHKDHVTLVRAFGRLARSRPDLTLVLPGGAGPSEADVSAAIRASGAGDQIRRTGR
                     IPWSDLAALYACATVVAVPSRFEGFGAPALEAMAGGVPVVVADATALPWVVGDAGLKF
                     RPGDAGQLTGELARVLDQRPVHDTLAAAGRVRAARFTWDHAAAELAAGYRAAAAVGET
                     GRA"
     gene            complement(2136..>2984)
                     /locus_tag="VNQ33_02970"
     CDS             complement(2136..>2984)
                     /locus_tag="VNQ33_02970"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWJ97093.1"
                     /translation="GPVDVVHGTAVAVPPTKAPLVMTIHDLAFLADRSQPTRHGLRFF
                     ERGTELARRHAQLVLVPSEATAVECREHGFDADRIRVVPWGVEVVPVSEEAVEAARRE
                     RGLDRPYVLFVGTMEPRKNLRGLIEAMVLLAGREVDLVLVGPEGWNEDVAALVGRLAG
                     TGIAVHALGFLPPDDLPPLFAGCAAFCFPSRREGFGMPVLEAMAHGAPVVTSSGTATA
                     EVAGDDALLVDPDDHEAIAEALARILDDPALADDLRARGRVRAEAYTWDRTAEATAAA
                     YAEVAP"
BASE COUNT          403 a         1071 c         1143 g          367 t
ORIGIN      
        1 atcgggcgag gccggtgcgc agcggcacca catggatgtc ggcggctgcc gccacctcgg
       61 ggagccgggc cttcggctgc atgtcgacga agcgcacgtt ggcgagcccg gccgcctggg
      121 ccctcaaccc ctcgcgggca acgccgccgc cgttgatcac gaacacgaca tcggggtcat
      181 cggccaggtg ggcggccgcc gccaagacga ggtccagcga ctgcgaccag cccacgttcc
      241 cggcgtacat gacgacggtc ttgccctcca ggccgaactc gcggcggtag gcgttctccc
      301 gctcagccgg gcggatgaag tcggtgtcga tgaagttcgg gatgacccgc accttgccgg
      361 gatcgcccgc cgcaccccgg cggcccgaga tcttggcggc gacgttggcc ttcaggtcct
      421 cggacaagac ggtgacggca tcggcgcgca ggtacgtctc ccgttcgagc cagctggcga
      481 cctcgatgac ccgggggtcg gtgatggcgc cgagctccac ggccacgtcc gggaagacgt
      541 cttggatgtt gaacacgaac gggacgcggt gcgccttggc gatggcccag cccgcggccc
      601 ccagggtgag cggcggtgac atcgccagca cggcgtcggg ctcggagcgg gtgaccacgc
      661 cggcgagacc gctcagcgcc gtgaacccgg cgaaggccag ggcccgtgcc gggatgttgc
      721 gcttgtcggt ggggaacggg tggacccggg tgatgcggcc ccactcggtg tcctcgtggc
      781 ggacgatctg cccctcccag ccgggttcga gggcgtggtg ctggtaccag ggcagcgagg
      841 tgacgatgtg cagccggttg ccccggtccg tcaggccctt ggcgatctgg gtcatcacct
      901 cgccggtcgg tgcgacgtcg gggacgaagt gcgggcacaa gacgaggagg ttcatgcccg
      961 gccagtctcg cccaccgcgg cggcggcccg gtagcccgcg gccagctcgg cggcggcgtg
     1021 gtcccacgtg aaccgggcgg cgcggacccg gccggcggcg gcgagcgtgt cgtggacggg
     1081 ccgctggtcg aggacccggg ccagctcacc ggtgagctgg cccgcatcgc ccggacggaa
     1141 cttcagcccg gcgtcgccga ccacccaagg gagcgcggtt gcgtcggcca ccacgacggg
     1201 gaccccgccc gccatggcct cgagtgcggg tgcgccgaac ccctcgaagc ggctgggcac
     1261 ggccacgacg gtggcgcacg cgtagagcgc ggcgaggtcg gaccacggga tccgaccggt
     1321 gcgccggatc tggtcgccgg cccccgaggc gcggatggcg gccgacacgt cggcctcgct
     1381 cggcccggcg ccaccgggca ggaccagcgt caggtcgggg cgggaccggg cgagccggcc
     1441 gaacgcccgc accagcgtga cgtggtcctt gtgggggtag gtgatggccg ggtacagcac
     1501 cacctgctcg ccgagccggt agcgctcgcg gaccgcggcg aggtcgccct ccggtaccgg
     1561 ctgcggcggt tcgatgccgt ggggcaccgt gcggacgcgg gcctccggca cggcgaggcg
     1621 ctcgaccacc gaggccgatg ccgggtcgct cggcgtgaag accatcgagg cccgccgagc
     1681 cgagcggggc agcatggtgg ccagccagcg gcgcttcacc gacgtgaagt tctccggcat
     1741 gaccagcggc tggaggtcgt ggatggtcag cgcggcgggg atgcggccga tagccggccc
     1801 cggtggcacg acaccgcccg cgtggtgcag cagctcgatg ttgcgtcggc ccgcctcgcg
     1861 ggcgagccac gtggcctcgg tggcgacccg ctcggccttg cggtggccgt cctggcgggc
     1921 cgtcaccgtc gggtaggcgg cgacgagatc ggggtgggca tcggcgaacg accgcagcgc
     1981 gaacagcgtg aggtgcaggt caggcggcag gtcagcggcc agcccccgca gcaggcgcgt
     2041 cgtgtactcc tcgctgccgc cgaccacgcc gggcaccaac cagagcaggt tgacgccgac
     2101 gcgcagcggc gtggtccggg tggcgctgag cgcggtcacg gcgccacctc ggcgtaggcc
     2161 gccgcggtcg cctcggccgt gcggtcccac gtgtaggcct ccgcccgcac ccggccgcgt
     2221 gcccggaggt cgtcggcgag cgccgggtcg tcgaggatgc gagccagcgc ctcggcgatg
     2281 gcctcgtggt cgtcggggtc gacgagcagg gcgtcgtcgc ccgccacctc ggccgtcgcc
     2341 gtccccgacg acgtcaccac cggcgcgccg tgggccatgg cctcgagcac gggcatgccg
     2401 aacccctcgc gccggctcgg gaagcagaac gcagcacacc cggcgaacag cggcggcagg
     2461 tcgtcgggcg gcaggaaccc gagcgcgtgc accgcgatcc cggtgccagc caggcgcccg
     2521 acgagcgcgg cgacgtcctc gttccagccc tccgggccga cgagcaccag atcgacctcg
     2581 cgtcctgcca gcagcaccat ggcctcgatc agaccccgca ggttcttgcg aggttccatc
     2641 gtcccgacga acagcacgta cgggcggtcg agtcctcgct cccgtcgagc ggcctcgacc
     2701 gcctcctcgg agaccgggac gacctcgacc ccccacggca cgacgcggat gcggtcggcg
     2761 tcgaacccgt gctcgcggca ctccaccgcc gtcgcctccg acggcaccag caccagctgg
     2821 gcgtggcggc gagccagctc cgtgccccgc tcgaagaagc ggaggccgtg gcgcgtgggc
     2881 tggctgcggt cggcgaggaa cgccaggtcg tggatggtca tgacgagcgg cgccttggtg
     2941 ggtgggacgg cgacggcggt gccgtgcacc acgtcgacgg gccc
//
LOCUS       DATMFS010000161         6726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_99883, whole genome shotgun sequence.
ACCESSION   DATMFS010000161 DATMFS010000000
VERSION     DATMFS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99883"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VNQ33_02975"
     CDS             <1..671
                     /locus_tag="VNQ33_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97094.1"
                     /translation="APARTVAPFRTVDAFAARPYDEGGCGLVGLAEGDAQRALVTAGT
                     LAPEDHIANILQRCEAEHRIEPVARLYQAALLRAPDTTGLRYWVARSRNGVQLRVIAS
                     QFAGSPEFKRRYGTLTNRRYVERIYQNVLGRAGDATGIAFWTKRLDTRKETRGGVLAG
                     FSESSEHVRRTDPLVQPAAAWFLMLDRAPTAAEQTTIAAFDRPRQDAALAIMALPEYA
                     ARVG"
     gene            734..1603
                     /locus_tag="VNQ33_02980"
     CDS             734..1603
                     /locus_tag="VNQ33_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ97095.1"
                     /translation="MAMPADIGIIDLMLDIPSGDQSEWYEFLKPQLREESKDLEFPAQ
                     YMFGDVPHPDPGDDPVDAVLKQMDHFGIEKAMIGVGFEDGRVDKRSAVVRYPDRFIGS
                     YSVDPNQGLAGVRDLVKAYETLGIKSAIAFPAGYLPQVPINDKRFFPIYAKCIELDIP
                     IACTTGICGPRVPSACQDTALIDEVCWFFPELKFVMRHGAEPWADLAVKLLLKWPNLS
                     YSTSAFAPKYYPQAIIDFANTRGADKVMYAGYFPMGLSLERIFADLANVPLKDEVWPK
                     FLRENAVRVFQLD"
     gene            1726..2796
                     /locus_tag="VNQ33_02985"
     CDS             1726..2796
                     /locus_tag="VNQ33_02985"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWJ97096.1"
                     /translation="MSDSNASRYPFTIPAGWFAVAESPDLEVGQTKAAYYFDTHLVLW
                     RDEERGVHVQDAFCPHLGAHLGHGGTVEGCEIMCPFHGWKFDAEGANTDIPYSERTNK
                     RAKVRTYPAMERNGFVYAWYHPHEEPPTWEVEAVLEIDGGEFSGPKRTNHVVQAGIQE
                     MAENAVDSAHFRYVHNVAEVPVIEKYESDGHRAYMESSQKFPTPRGVVDGRIDSMADG
                     PGVSIVRFSGIIDTLLVTASTPIDDSQTETRFHFYVRSLGDEAVNSSVGDAFAAEVDK
                     QFAEDMPIWEHKAHLTRPALADNDGPFMKFRKWYSQFYVEDESAVSIEDGRTVYPPPY
                     WPEKMDETPAKATASAKHGDNG"
     gene            complement(2828..3556)
                     /locus_tag="VNQ33_02990"
     CDS             complement(2828..3556)
                     /locus_tag="VNQ33_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97097.1"
                     /translation="MSSLVYAQSELKRTFRNFRFFIFSLGFPLALYFLIAGPNRHEKI
                     GGIPLPLYFMTGMMAFGTMIAVVSSGGRIAAERQLGWNRQLRITPLRAPSYLGAKVAS
                     GYAMAASSILLISLAGTLIGVRLTAGEWATLVALVLIGLIPFAAMGIAIGHLVSVDSL
                     GPLMGGLTSILALLGGAWGPIATSGALHTVSEAMPSYWLVEAGKTALGGSVWPLTGWL
                     VIGVWSVAMTVLAAWAYRRDTAKA"
     gene            complement(3553..4485)
                     /locus_tag="VNQ33_02995"
     CDS             complement(3553..4485)
                     /locus_tag="VNQ33_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97098.1"
                     /translation="MFPDQLTPGIELTGLTRSFGPVQAVKGIDLTVAPGEIVALLGPN
                     GAGKSTAIDMLLGLSQPDGGSVSVFGRPPREAIDAGSVGAMLQTGGLIRETTVRELLE
                     VMASLFPAPLPVEEVLALTGIGELAGRRTDKLSGGETQRVRFAIAIVSDPQLLVLDEP
                     TVAMDVSARHGFWEAMRTFAARGRTVLFATHYLEEADAWADRIVLMARGSIVADGPTG
                     EIKAMVGSRTIRATLPDVELQALTALPGVSAAERHGRTVVLTCVDSDAALRGLLAAQP
                     AACDIEVAAAGLEAAFLELTADQPGAAVGAEVPA"
     gene            4809..5963
                     /locus_tag="VNQ33_03000"
     CDS             4809..5963
                     /locus_tag="VNQ33_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97099.1"
                     /translation="MKRWLNVSWPVIVLGLLVSCGTGSSGRLESVASHQDWDGYVVPE
                     VQDVITSAVWNGREMVLVAFDHHGQRFENTVAAYDPTVGTWRDLHDLPSDLPVTVNAL
                     VASPQGVVATGVTCDPATSMIDGDGLACSPGDIRTFLLPNGDDAFVEIAAPSRRTTSQ
                     SIPSLAHGVSADGPYLWVDGSVFRLDQDRQWVPLGGTPVGMELCQLGQRLVGVATDPR
                     PPSGSPLDRLVEYDGTSHQWIPLDLPAQGNFGTLHCGLDAAWFELMNEEPARREDYLI
                     RSDGDPTRIKTPPNMLVGSITFDGTQLTTWASEVDPGTLPSDQRGQLYILDTSTATLV
                     SAADRSLPVHATLEVYPLDGISSLYATTVPSPDGDFSTSYEVDDTPKVAS"
     gene            complement(6126..>6726)
                     /locus_tag="VNQ33_03005"
     CDS             complement(6126..>6726)
                     /locus_tag="VNQ33_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWJ97100.1"
                     /translation="IKDSMMLWDPIRYAETCPSSDGACALVLGSEKVADEAAAHGRQP
                     AWILGTSMRSEPTLSAERDTVLPLGGALCAADVYKQAGIDNPRRDVDAVEMYVPFSWF
                     EPMWLENLGFAEPGEGWKMTEEGATAIDGDLPVNMSGGVLCTNPIGASGMLRFGEAAM
                     QVRGTAGEHQVDSVRKAVGHAYGGGSQFFAMWVVGKDKP"
BASE COUNT         1152 a         2409 c         2161 g         1004 t
ORIGIN      
        1 cagccccggc ccgcacggtc gccccgttcc gcacggtcga cgccttcgcc gcccgcccct
       61 acgacgaggg cggctgcggg ctcgtcgggc tcgcggaggg cgacgcccag cgcgccctcg
      121 tcacggccgg cacgctcgcg cccgaggacc acatcgccaa catcctgcag cgctgcgaag
      181 ccgagcaccg gatcgaaccc gtcgcccgcc tctaccaggc cgcgctcttg cgggcgcccg
      241 acaccaccgg cctgcgctac tgggtcgcgc ggagccgcaa cggggtgcag ctccgcgtca
      301 tcgcatccca gttcgccggc tctcccgagt tcaagcgccg ctacggcacg ctcaccaacc
      361 gccggtacgt cgagcgcatc taccagaacg tgctgggccg ggccggcgac gccaccggga
      421 tcgcgttctg gaccaagcgg ctcgacaccc gcaaggagac gcgcggcggc gtgctcgccg
      481 gcttctccga gtccagcgag cacgtacgcc gcacggaccc cctggtccag ccggccgccg
      541 cgtggttcct catgctcgac cgcgcgccga ccgcggcgga gcagacgacg atcgccgcct
      601 tcgaccggcc ccgccaggac gccgcgctcg ccatcatggc gctccccgag tacgccgccc
      661 gggtcggctg acccgctgct cccgccggga gcgaaacgag aacacgttct acttcggcgg
      721 gtagcctccc cgcatggcca tgcccgccga catcggcatc atcgacctca tgctcgacat
      781 cccctcgggc gaccagagcg agtggtacga gttcctcaag ccgcagctcc gtgaggagtc
      841 gaaggacctc gagttcccgg cgcagtacat gttcggcgac gtcccgcacc ccgacccggg
      901 cgacgatccc gtcgatgccg tgctgaagca gatggaccac ttcggcatcg agaaggcgat
      961 gatcggcgtg ggcttcgaag acggccgggt cgacaagcgc agcgcggtgg tccgctaccc
     1021 cgaccgcttc atcggctcct acagcgtcga ccccaaccag ggcctcgccg gcgtgcggga
     1081 cctggtgaag gcctacgaga cgctcggcat caagtcggcc atcgccttcc ccgccgggta
     1141 cctcccgcag gtgccgatca acgacaagcg gttcttcccg atctacgcca agtgcatcga
     1201 gctcgacatc ccgatcgcct gcaccaccgg gatctgcggg ccgcgggtcc cctcggcctg
     1261 ccaggacacc gcgctcatcg acgaggtctg ctggttcttc cccgagctga agttcgtgat
     1321 gcgccacggc gccgaaccgt gggccgacct ggccgtgaag ctgctgctca agtggccgaa
     1381 cctctcctac tcgacctcgg ccttcgcccc gaagtactac ccgcaggcga tcatcgactt
     1441 cgccaacacg cggggcgccg acaaggtcat gtacgccggc tacttcccga tgggcctcag
     1501 cctcgagcgg atcttcgccg acctggccaa cgtgccgctc aaagacgagg tgtggccgaa
     1561 gttcctgcgc gagaacgcgg tgcgggtctt ccagctcgac tgacgctccc gccctcggcc
     1621 agcgcgggac gggcggccat gggaccttcg tccctttcgc tccgggccga tcggccacta
     1681 cactagaaca cgttctagtt ttcgcccctg aaccaggtgg agtccatgtc cgacagcaac
     1741 gcaagccgct accccttcac catcccggcc gggtggttcg cggtggccga gtcccccgat
     1801 ctcgaggtgg gccagacgaa ggcggcgtac tacttcgaca cccacctggt cctgtggcgc
     1861 gacgaggagc gcggcgtcca cgtgcaggac gcgttctgcc cgcacctcgg cgcccacctc
     1921 ggccacggcg gcaccgtcga gggctgcgag atcatgtgcc cgttccacgg ctggaagttc
     1981 gacgccgagg gggccaacac cgacatcccc tactcggagc gcaccaacaa gcgggccaag
     2041 gtccggacgt acccggccat ggagcgcaac ggcttcgtct acgcctggta ccacccgcac
     2101 gaggagccgc cgacctggga ggtcgaggcc gtcctcgaga tcgacggcgg cgagttcagc
     2161 ggccccaaga ggaccaacca cgtggtccag gccggcatcc aggagatggc cgagaacgcg
     2221 gtcgactccg cccacttccg ctacgtgcac aacgtggccg aggtccccgt catcgagaag
     2281 tacgagtccg acggccaccg cgcctacatg gagtcgagcc agaagttccc gacgccccgc
     2341 ggtgtcgtcg acggccgcat cgactcgatg gccgacggtc ccggcgtgag catcgtccgg
     2401 ttcagcggca tcatcgacac gctcctcgtg acggcatcga cgccgatcga cgactcgcag
     2461 accgagaccc gcttccactt ctacgtgcgg agcctcggtg acgaagccgt gaactcgagc
     2521 gtcggcgacg ccttcgccgc cgaggtcgac aagcagttcg ccgaggacat gccgatctgg
     2581 gagcacaagg cccacctgac ccggccggcc ctcgccgaca acgacgggcc gttcatgaag
     2641 ttccgcaagt ggtactcgca gttctacgtc gaagacgaga gcgcggtctc gatcgaagac
     2701 ggccgcaccg tctacccgcc gccgtactgg cccgagaaga tggacgagac cccggccaag
     2761 gccaccgcca gcgccaagca cggcgacaac ggctgaccga acggctcgct cagggccgcg
     2821 gccccggtca cgccttggcg gtgtcgcgcc ggtaggccca cgccgccagg acggtcatcg
     2881 ccaccgacca gacgccgatc accagccacc cggtgagcgg ccagacgctg ccgccgaggg
     2941 cggtcttgcc cgcctccacc agccagtacg acggcatcgc ctcggacacc gtgtggagcg
     3001 cgcccgacgt ggcgatcggc ccccaggccc cgcccagcag ggcgaggatc gaggtcaagc
     3061 cgcccatgag cgggccgagc gagtcgaccg acaccaggtg cccgatggcg atgcccatgg
     3121 cggcgaacgg gatgaggccg atgaggacca gtgccacgag ggtggcccac tcccctgcgg
     3181 tcagccgcac gccgatgagc gtgccggcga ggctgatcag caggatcgag ctggccgcca
     3241 tcgcgtagcc cgacgccacc ttggcgccga ggtacgacgg agcgcgcagc ggcgtgatcc
     3301 gcagctgccg gttccacccc agctggcgct cggcggcgat ccggccgccc gacgagacga
     3361 cggcgatcat cgtgccgaac gccatcatcc cggtcatgaa gtagagcggc agcgggatgc
     3421 cgccgatctt ctcgtggcgg ttcggcccgg cgatcaagaa gtagagggcc agggggaagc
     3481 cgagcgagaa gatgaagaag cggaagttgc ggaaggtccg cttgagctcg gactgcgcgt
     3541 acacgagcga gctcatgccg gcacctccgc acccacggcc gcaccgggtt ggtcggccgt
     3601 gagctccaag aacgcggcct cgagcccggc ggcggcgacc tcgatgtcgc atgccgccgg
     3661 ttgggcggcg agcaggcccc ggagcgcggc atcggagtcg acgcaggtga gcacgaccgt
     3721 ccgtccgtgg cgctcggccg cactcacacc gggcagtgcg gtgagcgcct ggagctccac
     3781 gtcgggcagc gtcgcccgga tcgtgcggga gccgaccatg gccttgatct cgccggtcgg
     3841 gccgtcggcc acgatgctcc cccgcgccat caagacgatg cggtctgccc aggcgtcggc
     3901 ctcctcgagg tagtgggtgg cgaacagcac cgtcctgccg cgagcggcga aggtccgcat
     3961 cgcctcccag aacccgtggc gggcggagac atccatcgcc accgtcggct cgtcgagcac
     4021 gagcagctgc ggatccgaca cgatggcgat cgcgaaccgc acccgctgcg tctcaccgcc
     4081 cgagagcttg tcggtccgtc gcccggccag ctcgccgatg cccgtgagcg ccagcacctc
     4141 ctcgaccggc agcggcgccg ggaacagcga cgccatcacc tccagcagct cgcgcacggt
     4201 cgtctcccgg atgagcccgc ccgtctggag catcgccccg accgagccgg cgtcgatcgc
     4261 ctcccgcggc gggcggccga acacggagac gctcccgccg tcgggctgag acagcccgag
     4321 cagcatgtcg atggcggtgg acttgccggc gccgttcggt cccagcaggg cgacgatctc
     4381 gccaggagcg acggtgaggt cgatgccctt caccgcctgg accggaccga acgagcgggt
     4441 caatccggtg agctcgatcc ccggggtcag ctggtcagga aacatgacca ccacctttgc
     4501 accaatgacg tgcacagcac gcttgtgtgc cctcccgaca tccaacggtg ctcgctgagc
     4561 gcacggactc agctcgggtg acttgccccc ggtggatcca ggccgagcct gacttcagtg
     4621 gtcaggtgcg ctgtgtagac agcagccatc ctgggatcgt cacgttctgg ccggtgagga
     4681 cccaacacct cgcagcgttg ccatcgcctt gacctgtcct cgtatataac cgtattcagg
     4741 tacgctcgca atcgcatcaa aggcgaactc gcaatcgacg acaagtccgt cagcgtgagg
     4801 agtgcagaat gaagcgttgg ctgaacgtgt catggcccgt gatcgtccta gggttgctcg
     4861 tatcatgcgg caccgggagc agtggccgcc tcgagtcagt agcgagccac caggactggg
     4921 acggctacgt cgttcctgaa gttcaggacg tcatcacctc tgctgtgtgg aacggtcggg
     4981 agatggtgct cgtggccttc gaccaccacg gccagcggtt cgagaacacg gtggcggcgt
     5041 acgatccaac ggtcggaacc tggcgcgacc tccacgacct gccctcagac cttcccgtga
     5101 ccgtgaatgc tctggtggcg agcccacagg gggtcgtggc caccggcgtg acctgcgacc
     5161 cagcgacgag catgatcgac ggagacggcc tcgcctgctc gcctggcgac attcggacgt
     5221 tcctcctgcc caatggtgat gatgccttcg tcgagatcgc tgcaccgagt cgtcggacga
     5281 cctcgcagag catcccaagc cttgcccatg gggtcagcgc cgatggcccc tacctctggg
     5341 tggacgggtc cgtgttccga ctcgaccagg accgacagtg ggtccctctg ggtggtaccc
     5401 ccgtcgggat ggagctttgc cagctcggcc agcgactcgt tggagtcgcc accgatccac
     5461 gccccccatc aggctcgccg ttggaccggc ttgttgaata tgacggaacc tctcaccaat
     5521 ggattccact cgacctgcca gcgcaaggaa attttggaac attgcactgc ggcctcgacg
     5581 ccgcctggtt cgagcttatg aacgaggagc cagccaggcg ggaggactac ctcatcagga
     5641 gcgacggcga tcccactcgc atcaagaccc cacccaacat gctcgtcggc tcgatcacgt
     5701 tcgatggaac gcagctgacc acgtgggcaa gcgaagtaga tcccggcaca ttgccatctg
     5761 atcaacgcgg acagttgtac atcctcgata cctcaacggc cacgctcgtg agcgcagccg
     5821 accgatctct accagttcat gccacgctag aggtctatcc tctcgacggg atctcttcgc
     5881 tctatgcgac cacggtgcca tcccctgacg gagacttctc tacttcctac gaggtagacg
     5941 acacaccaaa ggtcgcctca tgacaataaa aactcttgga ccacgaccgc ggggtagtgc
     6001 gcttgcgcag ttggaccgtc tgcggcacac atctgacaag caaagacgtt cgatagctgt
     6061 aaatcagcta aatggattgt ttagcctgcg tcccatctgg agcggcccgt ccccgctcgt
     6121 cacacctagg gcttgtcctt gccgacgacc cacatggcga agaactggga gccgccgccg
     6181 taggcgtggc cgacggcttt gcggaccgag tcgacctggt gctcgccggc ggtgccccgg
     6241 acctgcatgg ccgcttcgcc gaagcggagc atgcccgagg cgccgatggg gttggtgcac
     6301 agcacgccgc ccgacatgtt gaccggaagg tcgccgtcga tcgcggtggc gccctcctcg
     6361 gtcatcttcc agccctcgcc gggctcggcg aagccgaggt tctcgagcca catgggctcg
     6421 aaccagctga acggcacgta catctcgacg gcgtcgacgt cgcggcgcgg gttgtcgatc
     6481 cccgcctgct tgtagacgtc ggcggcgcag agggcgccgc cgaggggcag caccgtgtcg
     6541 cgctcggccg acagggtggg ttccgaacgc atcgacgtgc cgaggatcca cgccggctgg
     6601 cggccatggg ccgcggcttc gtcggccacc ttctccgagc cgagcacgag ggcgcaggcg
     6661 ccgtcggatg acgggcaggt ctcggcgtag cggatcgggt cccagagcat catcgagtcc
     6721 ttgatg
//
LOCUS       DATMFS010000162         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_285874, whole genome shotgun sequence.
ACCESSION   DATMFS010000162 DATMFS010000000
VERSION     DATMFS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285874"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNQ33_03010"
     CDS             complement(<1..412)
                     /locus_tag="VNQ33_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWJ97101.1"
                     /translation="MAGTTPDKAPRTTRAETSVTSDLGALPHLMIRALKAMIADLAAR
                     NDDPQPPAHGLTAVHGIAARYIEANDDVTVVELAAHLRVTKQSASEIVAALEREGGVV
                     RRPHPADGRARVIELTDLGREGLVESRKRWGELIA"
     gene            480..2213
                     /locus_tag="VNQ33_03015"
     CDS             480..2213
                     /locus_tag="VNQ33_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97102.1"
                     /translation="MSHIQAHEAGADLPLHEQPKIHARRWWILAVLCLSLLIVFIGNS
                     SLNVAIPTLSRDLNATNSQLQWVIAVYSLVFAGLLFSSGATGDRYGRKGALQFGLVVY
                     LIACVGASEASSMSQLIAARAAMGVGAAFIMPSTLSILVNVFSPEERTKAIAIWASVT
                     GAAGAIGPVASGWLLGHFWYGAVFLVNVPILVFALVAGGTLIPRSKDPQQARLDPVGA
                     VLSIIGVVAIVYGLIEAPEHGWTSPTTLISFAVGVVVLTVFVFWELHNDEPMLDIRYF
                     KVPAFSTGTSGMILVFMAMYGVMFLVTQYFQLILGYSALSSAVRFLPMAPIMIIVAPM
                     TPRLSKRFGAHRTVAFGMLMVAVGLLMFRALSVDTSYWYLLVALIPLISGMALAMSPM
                     TAAIMSAVPPRRAGAGSAMNDATRELGAALGIAIMGSVASSQYTHHVDKLTASLSPQI
                     QDAARTSLADALGAAAELGGEAGRTLAVGTEHAFIDGIHFAVTVGSILAVIAAFAVYR
                     FLPRDLADEASLHGPVESMEDAAELGIAGVLPVFPDAVDDETADADQAAHDAGAGASQ
                     IGGLGQQPSPA"
     gene            complement(2387..>3389)
                     /locus_tag="VNQ33_03020"
     CDS             complement(2387..>3389)
                     /locus_tag="VNQ33_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97103.1"
                     /translation="LGSWWRRKEPKLLAAVAMIGVLSIAALVTGTNIPDSPEQGRLNF
                     YHWAFAISFFLLLVTGWLLARLAPVVTPRLRHGRPATAVAVSAVVIAGTAITPLVVQR
                     SSDRLLQPLPAAAVADLIDQLESSDALVGVSPPMLVLVNGDDRYIQVGDTVGARLAVD
                     GWPVLFPPSSKGFVHPDRIADPCAVQRALVISLVRGDVAEPPGTKAADVDAAPTLDRA
                     ALDRLIEQAYGVPVEFGPDLQAALDALPGDQGDLIGATIAFRLGFRPEEVLLARFNLD
                     LLIDHPIAEPALDHDDLVALRGSLPDGAATLVAEDLTAHVLDRHQLEQFRPDFFEGC"
BASE COUNT          478 a         1233 c         1178 g          500 t
ORIGIN      
        1 cggcgatcag ctcgccccac cgcttgcggg actcgacgag gccctcgcgg ccgaggtcgg
       61 tgagctcgat gacccgggcc cgcccgtcgg cggggtgggg acgccggacc acgccgccct
      121 cgcgctcgag cgcggcgacg atctccgagg cggactgctt ggtcacccgg aggtgggcgg
      181 ccagctccac cacggtcacg tcgtcgttcg cctcgatgta gcgggccgcg atcccgtgca
      241 cggccgtgag cccgtgcgcc ggcggctgcg ggtcgtcgtt gcgagccgcc aggtcggcga
      301 tcatcgcctt cagggcgcgg atcatcaggt gcggcagcgc gcccaggtca gacgtgaccg
      361 atgtctcggc gcgcgtggtg cgcggagctt tgtcgggggt cgtgccggcc atatagtcag
      421 gggaccttac ttgtttgcga tcgaagccac cgctcgaggc gggcggcagg agcgaagccg
      481 tgtcccacat ccaagcgcac gaagcgggag ccgacctccc gctccacgag cagccgaaga
      541 tccacgcccg ccgctggtgg atcctggccg tgctgtgcct cagcctgctc atcgtcttca
      601 tcggcaactc gagcctcaac gtggccatcc ccacgctgtc gcgggacctg aacgcgacca
      661 actcccagct gcagtgggtg atcgcggtct actcgctcgt gttcgcgggc ctcctgttct
      721 ccagcggcgc caccggcgac cgctacggcc gcaagggcgc cctgcagttc gggctcgtcg
      781 tgtacctgat cgcctgcgtc ggcgcctccg aggccagctc gatgtcccag ctcatcgccg
      841 cccgagcggc catgggcgtg ggagcggcgt tcatcatgcc gtcgacgctc tcgatcctcg
      901 tgaacgtgtt ctcgcccgag gagcgcacga aggccatcgc catctgggcg agcgtgaccg
      961 gcgccgccgg cgccatcggc ccggtcgcca gcggctggct cctcggccac ttctggtacg
     1021 gcgccgtgtt cctcgtcaac gtgcccatcc tcgtcttcgc cctggtggcc ggcgggaccc
     1081 tcatcccccg ctccaaggac ccgcagcagg cccgcctcga ccccgtcggc gccgtgctgt
     1141 cgatcatcgg cgtcgtcgcc atcgtctacg gcctcatcga ggcgcccgag cacggctgga
     1201 ccagcccgac cacgctcatc tccttcgccg tcggcgtcgt ggtcctgacc gtcttcgtct
     1261 tctgggagct gcacaacgac gagccgatgc tcgacatccg ctacttcaag gtcccggcgt
     1321 tcagcacggg cacgagcggc atgatcctcg tgttcatggc catgtacggc gtgatgttcc
     1381 tggtcaccca gtacttccag ctgatcctcg gctacagcgc cctcagctcg gctgtccgct
     1441 tcctcccgat ggcgccgatc atgatcatcg tggcgccgat gacgccgcgg ctctccaagc
     1501 ggttcggcgc ccaccgcacg gtggcgttcg ggatgctcat ggtggccgtc ggcctcttga
     1561 tgttccgggc gctgagcgtg gacacgtcgt actggtacct gctcgtcgcc ctcatcccgc
     1621 tcatcagcgg catggccctc gccatgtccc cgatgaccgc agcgatcatg tcggccgtcc
     1681 cgccccgccg ggccggcgcc ggctcggcca tgaacgacgc cacccgggag ctgggcgccg
     1741 cgctcggcat cgccatcatg ggcagcgtcg ccagctcgca gtacacgcac cacgtcgaca
     1801 agctcaccgc gagcctctcg ccccagatcc aagacgccgc ccgcacctcg ctggccgacg
     1861 ccctgggcgc cgccgccgag ctgggcggcg aggcgggccg gacgctcgcc gtcggcaccg
     1921 agcacgcgtt catcgacggc atccacttcg cggtgacggt cggctcgatc ctcgccgtca
     1981 tcgccgcctt cgcggtgtac cgcttcttgc cccgcgacct cgccgacgag gcctcgctgc
     2041 acggcccggt cgagtcgatg gaagacgccg ccgagctcgg catcgccggc gtgctccccg
     2101 tcttccccga cgccgtcgac gacgagaccg cagatgccga ccaggccgcc cacgacgccg
     2161 gcgccggcgc cagccagatc ggcggcctgg gccagcagcc ctcccccgcc tgaccactcc
     2221 cgaggcgcag agcccgggcc acgtgaccga agttggtgtg cgaactgatg cagtgcctcg
     2281 gttgccacac caacttcggg cgaggccggc tcaaggagca agggacgacc gatgacggcg
     2341 gcggcacgag cagcggcgag ggaaccggct ctgcgacgag cggccttcag cacccctcga
     2401 agaagtcggg gcggaactgc tcgagctggt ggcggtcgag gacgtgggcc gtgaggtctt
     2461 cggccaccag ggtggcggcg ccgtcgggga gcgaaccgcg gagggcgacg agatcgtcgt
     2521 ggtcgagtgc gggctcggcg atcgggtggt cgatcagcag atcgaggttg aagcgcgcga
     2581 gcaggacctc ctcgggccgg aagccgaggc ggaacgcgat cgtggccccg atgaggtcgc
     2641 cctggtcgcc gggcagggcg tcgagggcgg cctggaggtc cggtccgaac tcgaccggta
     2701 cgccataggc ctgctcgatc agccggtcga gcgccgctcg gtcgagcgtc ggggccgcat
     2761 cgacgtcggc cgccttcgtg cccggcggct cggcgacgtc gccgcggacg aggctgatca
     2821 cgagcgcccg ctggacagcg cacgggtcgg cgatccggtc ggggtgcacg aagcccttcg
     2881 agctcggcgg gaacaagaca ggccaaccgt cgacggcgag gcgagcgccg acggtgtcgc
     2941 caacctggat gtagcggtcg tccccattga cgaggacgag catcggcggc gagacgccca
     3001 cgagggcgtc agacgactcg agctggtcga tcaggtccgc caccgcggcc gccggcagcg
     3061 gctgcagcag gcggtcgctc gaccgttgga ccacgagggg cgtgatcgcg gtgcccgcga
     3121 tcaccaccgc cgacacggcg acggcggtgg ccggccgccc gtgcctcagc ctcggggtga
     3181 cgaccggtgc gagccgggcg agcagccagc ccgtcacgag cagcaggaag aacgagatgg
     3241 cgaaggccca gtggtagaag ttcagccggc cctgctccgg cgagtccggg atgttggtgc
     3301 cggtgaccag cgcggcgatc gacaagacgc cgatcatggc gacggcggcg aggagcttcg
     3361 gctccttgcg ccgccaccag ctgcccagc
//
LOCUS       DATMFS010000163         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_309084, whole genome shotgun sequence.
ACCESSION   DATMFS010000163 DATMFS010000000
VERSION     DATMFS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309084"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VNQ33_03025"
     CDS             <1..500
                     /locus_tag="VNQ33_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ97104.1"
                     /translation="EIIRLLAPFEPSITIVRRSPGAVPGADRVVTADRLDEVLPEAEI
                     VILAAAATDETSRLIDARRIELMDENAILVNIARGSLVDAEALAAALERGHLKGAGID
                     VTDPEPLPDGHPLWTAPRVVITCHSADTEEMVAPLLASRVESNMRALLGDGAFIGVVD
                     PAAGY"
     gene            595..>2595
                     /locus_tag="VNQ33_03030"
     CDS             595..>2595
                     /locus_tag="VNQ33_03030"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWJ97105.1"
                     /translation="MLRAVRAIGIVALVALVALGPPPASAAADDVPTALYVATDGHDD
                     ASCGAIDAPCSTVSEGVSRAKAAIAGGALEVIVRLAAGTYDENVDIGVVASGSSLSLI
                     GTGASTTVIDGLGSASTPASVITVASGTVTLGGLSITGGYAASGGGIHNAAGTLTISE
                     VAVRGNTANAAGNYGGGGGGIYNGTGTLAILDSEVSNNTSAGQGYGGGIFGLDGGTVT
                     IVDSTVSGNTASYYVGGIHNRGLLTITRSTVSDNTTQRNFGGGISNYGGTLVMTDSTM
                     SGNRALGANTGKGGAIYNRGPATVINSTLSGNAATFGGGIHNLGSGALTVTYSTLAEN
                     TATRGGGGYRWEGGTITISNTILFASTCDAYSVIIDGGYNVSDTCTFYAPSGTSIAGS
                     STIGLAPLAANSSTGPQTMAIDTTSSAHHLVPGCTGTDARGLDRPGFGGTANCDAGAY
                     ELQGVAPVVTTDPQSQTFAIGSDATFTSAASGTPAPTVQWQAYNDDAWGDVPGATSTT
                     LTLASVTHSMTGSQYRAVFTNGVATDATTAAAMLTVPVGALDYLVLSPTAPKIVAGGS
                     QVFTAEGFDAFDNALGDVTAVTVFTGSADMVCAAATCTAIAPGSYTVTGTSGTATGTA
                     SVTVESLPDVPLPATRPVASGTLTDVPGTIRSDGRFTATASGF"
BASE COUNT          421 a          880 c          906 g          388 t
ORIGIN      
        1 aggagatcat ccgtctgctc gcgccattcg agccgtcgat cacgatcgtc cggcgctcgc
       61 cgggggcggt ccccggcgcg gaccgcgtcg tcaccgccga ccggctcgac gaggtgctcc
      121 ccgaggcgga gatcgtcatc ctcgcggccg cggcgacgga cgagacctcg cggctcatcg
      181 acgcgcggcg catcgagctg atggatgaga acgcgatcct cgtgaacatc gcgcgcggct
      241 cgctcgtcga cgcggaggcg ctcgccgccg cgctcgagcg cgggcacctc aagggcgccg
      301 ggatcgatgt gacggacccg gagccgctgc ccgacgggca tccgctctgg accgcgccac
      361 gcgtggtcat cacctgccac tccgcggaca ccgaggagat ggtcgccccg ctgctcgcga
      421 gccgggtcga gtcgaacatg cgggcgctgc tcggcgacgg cgccttcatc ggggtcgtcg
      481 atccggcagc ggggtactga gcggacgatt gggtttcggc gtccgcgggg cgcggccggc
      541 ctctctcggc gtctgggcta tggtttgtaa ctcggcccaa aggcgggaag tcacatgctc
      601 agagcagtca gggccatcgg catcgtcgcg ctcgtcgcat tggtcgcgct gggtcctcct
      661 cccgcatcgg cggcggcaga cgacgtgccg acggcgctgt acgtcgccac ggatgggcac
      721 gacgatgcgt cgtgtggcgc gatcgatgcg ccctgctcga ccgtgagcga aggcgtgtct
      781 cgcgccaagg cggcgatcgc tggtggcgcg ctcgaggtca tcgtgcgcct cgccgccggc
      841 acgtacgacg agaacgtcga tatcggtgtc gtagcctccg gaagcagcct gagcttgatc
      901 ggcaccggcg catccacgac cgtcatcgac ggtctgggca gcgcctcgac gcccgcgagc
      961 gtcatcaccg tcgcgagcgg gaccgtcacg ctcggcggcc tctcgatcac cggcgggtac
     1021 gcggcgagcg gcggcgggat tcacaatgcc gcgggcacgc tgacgatctc cgaggtcgcc
     1081 gtgaggggca ataccgcaaa tgccgctggc aactacggtg gcggcggtgg tggcatctac
     1141 aacgggaccg gcacactggc gattctcgac tccgaggtct cgaacaacac gtcggcgggc
     1201 cagggctacg gcggcggcat cttcggccta gacggtggga ctgtcacgat cgttgactcc
     1261 acagtgtcag gaaacaccgc cagctactac gtgggcggca tccacaaccg cggcctgctg
     1321 acgatcaccc gctccacggt gtcggacaac accacccagc ggaacttcgg cggcggtatc
     1381 tccaactacg gcggcacact ggtcatgacc gactcgacga tgtcgggcaa cagggcactc
     1441 ggggcgaaca ccggcaaagg gggcgccatc tacaacagag ggccggccac ggtgatcaac
     1501 tcgacgctgt ccggtaatgc cgcgactttc ggcggcggga tccataacct cggcagcggc
     1561 gcactcaccg tgacctactc cacgctggcc gagaacacgg cgacgagggg cggcgggggt
     1621 taccgctggg agggtggcac gatcaccatc tcgaacacca tcctgttcgc gtcgacgtgc
     1681 gacgcctact cggtgatcat cgacggcggc tacaacgtga gcgatacgtg cacgttctac
     1741 gccccgagcg ggacgagcat cgccggcagc agcacgatcg gcctggcgcc gctggcggcg
     1801 aatagctcca ccggaccgca gacgatggcg atcgacacca ccagctctgc gcaccacctc
     1861 gtgccgggat gcaccggcac cgatgcccgg ggcctggacc ggccggggtt cggcgggaca
     1921 gccaactgcg acgccggcgc ctacgagctg cagggggtcg ctccggtggt gaccaccgac
     1981 ccgcagtcgc agacgttcgc gatcggctcg gatgcgacgt tcacctcggc agcatcgggg
     2041 acaccggcgc cgaccgtgca gtggcaggcc tacaacgatg acgcttgggg cgatgtaccc
     2101 ggagctacgt cgaccacgct gacgctggcg agcgtgacgc actcgatgac cggtagccag
     2161 taccgcgcag tgttcaccaa tggagtggcc acggatgcga cgacggctgc ggcgatgctg
     2221 accgtgccag tgggggcgct ggactacttg gtgctgtcac cgaccgcacc gaagatcgtg
     2281 gcgggtggct ctcaggtgtt caccgccgaa ggctttgacg ccttcgacaa cgcgctcggg
     2341 gacgtgaccg ccgtcacggt gttcacggga tccgctgaca tggtgtgtgc ggcagcaacc
     2401 tgcacggcga ttgctccggg ctcctacacg gtgacgggca cgtcgggcac cgcgacgggg
     2461 accgcctcgg tcacggtgga atcgttgccc gacgtgccgt tgcccgcgac caggcccgtt
     2521 gcgtccggaa cgctgaccga tgttccgggg acgatccggt ccgatggccg gttcaccgcc
     2581 acggcatccg ggttc
//
LOCUS       DATMFS010000164         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_7232, whole genome shotgun sequence.
ACCESSION   DATMFS010000164 DATMFS010000000
VERSION     DATMFS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7232"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VNQ33_03035"
     CDS             complement(<1..690)
                     /locus_tag="VNQ33_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWJ97106.1"
                     /translation="MSETTGNAATRIAVIGQGYVGLPLAVRAVEQGYDVVGYDLDKAR
                     IEQLASGSSFIEDIPDERLQAILATGRFHPSTDGDDLAGFDIAVISVPTPLREGMPDL
                     TYIEASAAQLGSHLKPGACVILESTTYPGTTEELMLPILEDASGLKAGTDFQAGYSPE
                     RIDPGNKTYTLETTPKVVSGIDEASLARVQGFYDDIVVKTVPVRSPKEAELTKLLENT
                     FRHVNIALVNEL"
     gene            768..2240
                     /locus_tag="VNQ33_03040"
     CDS             768..2240
                     /locus_tag="VNQ33_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWJ97107.1"
                     /translation="MPGYHPLTPADLEVAVSPRALQNLVGGAAADAADGATIDLVDPS
                     TGEVFATSPRSGAADVDAACRAAATAFEAWRDTTPSDRQRALLKLADLVEGHADELVA
                     LESENTGKPIGLTAEEEVPPMVDQIRFFAGAARVLEGTSAGEYLAGHTSWIRREPVGV
                     CAQVTPWNYPMMMAVWKIAPALAAGNTVVLKPSDTTPVSTVRLAELAAEAFPEGVLNV
                     ICGDRETGAALVSHKIPQMVSITGSVRAGMEVMRSASDDLKNVHLELGGKAPVIVFED
                     ADVEAAAEAIAIAGYFNAGQDCTAATRVLAAPAVHDELAAALTAQANGHTTGPPSNPD
                     ADFGPLNNADQLERVAGIVSRVPDHASVLAGGSQVGGAGYFFEPTVVAGLRQDDELIQ
                     NEIFGPVITLQSFADEDEALRYANGVEYGLASSVFTTDHARALRVSRRLDFGCVWINT
                     HIPLVAEMPHGGFKHSGTGKDLSRYGLEDYTRIKHVMSAF"
     gene            2240..>2451
                     /locus_tag="VNQ33_03045"
     CDS             2240..>2451
                     /locus_tag="VNQ33_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWJ97108.1"
                     /translation="MRVLLVGAGGVGSAFVKIAARRDFIESLVVADIDAGRASEVAAA
                     FGPDAKGIALDASDAGAVEGVLRAEAS"
BASE COUNT          341 a          876 c          859 g          375 t
ORIGIN      
        1 gagctcgttg accagggcga tgttgacgtg gcggaaggtg ttctcgagca gcttggtgag
       61 ctcggcctcc ttcggtgagc ggaccggcac cgtcttcacg acgatgtcgt cgtagaagcc
      121 ctggacccgg gccaggctcg cctcgtcgat gccggacacg accttgggcg tggtctcgag
      181 cgtgtaggtc ttgttccccg ggtcgatgcg ctcgggggag tagccggcct ggaagtcggt
      241 gccggccttg agcccgctcg cgtcctcgag gatcggcagc atcagctcct cggtggtccc
      301 cgggtaggtg gtggactcga ggatcacgca ggcgcccggc ttcaggtggc tccccaactg
      361 cgcggcggag gcctcgatgt aggtcaggtc gggcatgccc tcgcgcagcg gcgtcggcac
      421 ggagatgacg gcgatgtcga agcccgccag gtcatcgccg tcggtgctcg ggtggaaccg
      481 gccggtggcc aagatcgcct gcaggcgctc gtcggggatg tcctcgatga acgagctccc
      541 cgatgcgagc tgctcgatgc gggccttgtc caggtcgtac ccgaccacgt cgtagccctg
      601 ctccacggcg cggaccgcca ggggaagacc cacgtagccc tgcccgatga ccgcgatccg
      661 agtcgcggcg ttgccggtgg tttcgctcat ggtctacccg ttccctcccc ctggctgatg
      721 cggccgcaaa cggtaccacc ggcccatcgt cgggctcgtc tttcgagatg ccggggtacc
      781 atccgctcac gcccgccgac ttggaggtcg ccgtgtcccc tcgtgccctg cagaacctgg
      841 tgggaggtgc cgccgccgac gctgcggacg gcgccaccat cgacctcgtc gatccctcca
      901 ccggcgaggt gttcgcgacc tcgccgcgct caggcgccgc cgacgtcgac gccgcttgcc
      961 gggcggccgc cacggcgttc gaggcctggc gggacacgac ccccagcgac cgccagcgcg
     1021 ccctcctgaa gctggccgac ctcgtcgagg ggcatgccga cgagctggtc gcgctggagt
     1081 ccgagaacac gggcaagccg atcggtctga ccgccgagga ggaggtgccg ccgatggtcg
     1141 accagatccg gttcttcgcc ggcgccgccc gcgtcctcga gggcacctcg gcgggggagt
     1201 acctggccgg ccacacgtcg tggatccgcc gggagcccgt cggcgtgtgc gcccaggtca
     1261 cgccgtggaa ctacccgatg atgatggccg tctggaagat cgcccccgcg ctggcggcgg
     1321 gcaacaccgt cgtgctgaag ccctcggaca ccaccccggt gtcgaccgtc cggctggccg
     1381 agctggccgc cgaggcgttc cccgagggcg tgctcaacgt gatctgcggc gaccgggaga
     1441 ccggcgcagc cctcgtgtcg cacaagatcc cgcagatggt ctcgatcacc gggtcggtgc
     1501 gtgccggcat ggaggtcatg cgcagcgcct ccgacgacct gaagaacgtc cacctggagc
     1561 tgggcggcaa ggcaccggtg atcgtgttcg aggatgcgga cgtcgaggcg gccgctgaag
     1621 ccatcgccat cgccgggtac ttcaacgccg gccaggactg cacggccgcc acccgggtgc
     1681 tggccgcgcc cgccgtccac gacgagctgg ccgcggcgct caccgcgcag gccaacggcc
     1741 acaccaccgg tccgccgtcg aaccccgatg ccgacttcgg cccgctcaac aacgccgacc
     1801 agctcgagcg ggtcgcgggc atcgtgtccc gggtgcccga ccacgcctcg gtgctggccg
     1861 gcgggtccca ggtcggcggc gccggctact tcttcgaacc gacggtcgtc gccgggctgc
     1921 gccaggacga cgagctcatc cagaacgaga tcttcggccc ggtcatcacc ctgcagtcgt
     1981 tcgccgacga ggacgaggcg ctccgctacg ccaacggcgt cgagtacggg ctggcctcca
     2041 gcgtgttcac cacggaccac gcccgggcgc tgcgggtctc ccgccggctc gacttcgggt
     2101 gcgtctggat caacacccac atcccgctcg tggccgagat gccccacggc gggttcaagc
     2161 actccggcac cggcaaggac ctcagcaggt acggcctcga ggactacacc cgcatcaagc
     2221 acgtgatgag cgccttttga tgcgcgtcct gctggtggga gcgggcggtg tcgggtccgc
     2281 cttcgtgaag atcgcggcgc gacgggactt catcgagtcg ctcgtcgtcg ccgacatcga
     2341 cgccggccgt gcgtcggagg tggcggccgc gttcggcccc gacgccaagg gcatcgccct
     2401 cgatgcgtcc gacgcgggag ccgtcgaggg cgtcttgcgc gccgaggcgt c
//
LOCUS       DATMFS010000165         2700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_52634, whole genome shotgun sequence.
ACCESSION   DATMFS010000165 DATMFS010000000
VERSION     DATMFS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52634"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VNQ33_03050"
     CDS             <1..410
                     /locus_tag="VNQ33_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWJ97109.1"
                     /translation="EPVGTYLVNICTNISCQIMGGEELLHHAEHTLGIKAGSTTTDGL
                     FTIEDVECIAACTEAPCLQVNYRYRHRITIEEFDQLIEDLRAGKLADEVPPHGTLART
                     RQHIPEDRRAGNALPSGGPEPVWLARNAEGGEA"
     gene            410..1783
                     /gene="nuoF"
                     /locus_tag="VNQ33_03055"
     CDS             410..1783
                     /gene="nuoF"
                     /locus_tag="VNQ33_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWJ97110.1"
                     /translation="MAVTDAPKIITTRFDQPDGHTLDGYLRTGGYAGIKAALAKKPAD
                     VVEEVKTASLLGRGGAGFPAGVKWGFCPPGVFPRYLVVNGDESEPGTYKDRLLMERDP
                     HQLIEGVLIACYAVGCSQAFLYVRGEMAFAQERIAAALNEAYEAGYVGKNILGSDFSV
                     DIVLHWGAGAYIVGEETALIESLEGNRGMPRLKPPFFPAAKGLYLQPTIVNNVETLAN
                     LPWIISNGGASFAALGAENSRGMRMFAVSGHVKNPGVYEVEFGVTTFRDLLYAPVYGG
                     GIRGGKELKAFIPGGASAPWLYPEHLDTPLEAGVVGKMGTMLGSGAIVVFDEDTDMVK
                     ACLRLVRFFARESCGKCTPCREGTSWLEKILERILDGHGRPEDLDLLLDVCDNISVGM
                     NWPPKQTTICPLGQSATTPVASAIMRFQDEFEAYIGAPSEITVEVKGPAFVGPYDAAA
                     KEVASVD"
     gene            1776..>2700
                     /locus_tag="VNQ33_03060"
     CDS             1776..>2700
                     /locus_tag="VNQ33_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWJ97111.1"
                     /translation="MTDVETSVSITVDGKQVDAKPGELVIDAAERNGVYIPRFCYHHR
                     MEPVGMCRMCLVEVDTGRGPALQPSCMLTVAPDMVVETESPVAKKAQDGILEFLLINH
                     PLDCPVCDKGGECPLQDHTIAFGPGESRFVEEKRHYEKPISISDNVYLDRERCILCDR
                     CTRFAKDVAGDTLIHFQDRGNGTQVNTFPDHPFASYFSGNTVQICPVGALTAKPYRFK
                     ARPWDLDVVESTCMGCSVGCRLVVDASRNEVLRYVGVDSDPVNWSWLCDKGRFGFEAV
                     GAEARLSAPLAKDATGTLAETRWADALTAAAR"
BASE COUNT          439 a         1030 c          851 g          380 t
ORIGIN      
        1 gggagcccgt cgggacctac ctcgtgaaca tctgcacgaa catcagctgc cagatcatgg
       61 gcggcgagga gctgctgcac cacgccgagc acaccctcgg catcaaggcc ggcagcacca
      121 ccaccgacgg gctcttcacc atcgaagacg tcgagtgcat cgccgcgtgc accgaggccc
      181 cgtgcctcca ggtcaactac cgctaccggc accgcatcac catcgaggag ttcgaccagc
      241 tgatcgagga cctgcgcgcc ggcaagctcg ccgacgaggt cccgccgcac ggcaccctcg
      301 cccgcacccg ccagcacatc cccgaagacc gccgggccgg caacgccctg ccgtcggggg
      361 gccccgagcc ggtgtggctg gctcgcaacg ccgaaggggg cgaggcctga tggccgtcac
      421 cgacgcaccc aagatcatca cgacccggtt cgaccagccc gacggccaca cgctcgacgg
      481 ctacctgcgc accggcggct acgcgggcat caaggccgcg ctggccaaga agccggccga
      541 cgtcgtcgaa gaggtcaaga ccgcttcgct gctcggccgg ggcggcgccg gcttccccgc
      601 cggcgtcaag tggggcttct gcccgcccgg cgtgttcccg cgctacctcg tggtcaacgg
      661 cgacgagtcc gagccgggca cctacaagga ccgcctcctc atggagcgcg atccgcacca
      721 gctcatcgag ggcgtgctca tcgcctgcta cgccgtcggc tgcagccagg cgttcctcta
      781 cgtccgtggc gagatggcct tcgcccagga gcgcatcgct gccgccctga acgaggccta
      841 cgaagccggc tacgtcggca agaacatcct cgggtccgac ttctcggtcg acatcgtcct
      901 gcactggggt gccggcgcct acatcgtggg cgaggagacg gcgctgatcg agagcctcga
      961 gggcaaccgg ggcatgcccc gcctgaagcc cccgttcttc ccggccgcca agggcctgta
     1021 cctccagccc accatcgtca acaacgtcga gacgctggcc aacctcccgt ggatcatcag
     1081 caacggcggc gcgtcgttcg cggcgctggg cgccgagaac tcgcgcggca tgcgcatgtt
     1141 cgcggtgtcg ggccacgtga agaaccccgg cgtctacgag gtcgagttcg gcgtcacgac
     1201 gttccgggac ctgctctacg cgcccgtgta cggcggcggc atccgcggcg gcaaggagct
     1261 caaggcgttc atccccggcg gcgcatcggc accgtggctg taccccgagc acctcgacac
     1321 gccgctcgaa gccggcgtcg tcgggaagat gggcaccatg ctcggctcgg gcgccatcgt
     1381 cgtgttcgac gaggacaccg acatggtcaa ggcctgcctg cggctcgtgc gcttcttcgc
     1441 ccgggagtcc tgcggcaagt gcacgccgtg ccgcgagggc acgagctggc tggagaagat
     1501 cctcgagcgg atcctcgacg gccacggccg ccccgaggac ctcgacctcc tgctcgacgt
     1561 gtgcgacaac atctcggtcg gcatgaactg gccgccgaag cagaccacca tctgcccgct
     1621 cggccagagc gccaccacgc cggtcgcctc ggcgatcatg cgcttccagg acgagttcga
     1681 ggcctacatc ggtgcgccct cggagatcac cgtcgaggtc aagggcccgg ccttcgtcgg
     1741 cccctacgac gcagccgcga aggaggtggc ctccgttgac tgacgtcgag acctccgtgt
     1801 ccatcaccgt cgacggaaag caggtcgacg ccaagccggg cgagctcgtc atcgacgccg
     1861 ccgagcgcaa cggcgtctac atcccccgct tctgctacca ccaccgcatg gagcccgtcg
     1921 gcatgtgccg gatgtgcctg gtggaggtcg acaccggccg cggcccggcc ctccagccca
     1981 gctgcatgct caccgtggcg cccgacatgg tcgtggagac cgagtcgccg gtggccaaga
     2041 aggcccaaga cggcatcctc gagttcctcc tgatcaacca cccgctcgac tgcccggtgt
     2101 gcgacaaggg cggcgagtgt cccctccagg accacaccat cgccttcggc cccggcgaga
     2161 gccgcttcgt cgaggagaag cggcactacg agaagccgat ctcgatctcc gacaacgtct
     2221 acctcgaccg cgagcgctgc atcctctgcg accgctgcac ccgcttcgcc aaagacgtcg
     2281 ccggtgacac gctgatccac tttcaagacc gcggcaacgg cacccaggtc aacaccttcc
     2341 cggaccaccc gttcgcctcg tacttctccg gcaacaccgt gcagatctgc ccggtgggcg
     2401 ccctcaccgc caagccgtac cgcttcaagg cccgcccgtg ggacctcgac gtcgtcgagt
     2461 ccacctgcat gggctgctcg gtcggctgcc gcctcgtggt cgacgcctcg cgcaacgagg
     2521 tgctccgcta cgtcggcgtg gactcggacc cggtcaactg gagctggctc tgcgacaagg
     2581 gccgcttcgg cttcgaggcc gtcggcgccg aggcccgcct cagcgccccg ctcgccaagg
     2641 acgccaccgg caccctcgcc gagacccgct gggccgacgc cctcaccgcc gccgcccggg
//
LOCUS       DATMFS010000166         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193328, whole genome shotgun sequence.
ACCESSION   DATMFS010000166 DATMFS010000000
VERSION     DATMFS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193328"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(561..1472)
                     /locus_tag="VNQ33_03065"
     CDS             complement(561..1472)
                     /locus_tag="VNQ33_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWJ97112.1"
                     /translation="MARRSGLGKGLGALIPNDLTAPSDDATLRELQVSQIEPNQHQPR
                     AQFDEEALVSLTDSIRELGVLQPILVRPSGHDRYELIAGERRWRASKRAGLQTIPAIV
                     RKIDETTSLEQALVENLHREDLHPMEEAAAYQQLIEEFNLSHEDVARRVGKSRSAVSN
                     MLRLFQLPPSIQKLVADRQLSAGHARALLGTPDRSFQEALARRAVAEQLNVREVEEAV
                     RARSGEVEADDDPEPGTKPTPGVAGRRLRPPGLLELEGLLSDRLDTRVKVSMGAKRGK
                     VTIDFASLEDLERIYRAMTAGPESPTA"
     gene            complement(1459..2205)
                     /locus_tag="VNQ33_03070"
     CDS             complement(1459..2205)
                     /locus_tag="VNQ33_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97113.1"
                     /translation="MANQKGGVGKTTTTVNLGAALADLGYRVLVVDLDPQGNASTGLG
                     INIRGLQASMYDVILSDVPIEDCVEASSVKNLFVAPASLDLAGAEIELVPAFSRELRL
                     KQALHEVRDDYDYVLIDCPPSLGLLTINGMAAASEVLVPIQCEYYALEGLGQLMRNVG
                     LVTKNLNPVLDISTIVLVMYDARTKLSDQVAQEVRAHFGDKVCKIMIPRTVRLSEAPS
                     FGQPITAFDPSSRGAVAYRELAREVSGGPA"
BASE COUNT          354 a          801 c          771 g          356 t
ORIGIN      
        1 cccgacgctc atcgtgttcc aagacgggca gccggtgcac cgcttccagg gcgcgtcggg
       61 caaggccggc ctcctcgagc agctcagcga cttcctctga tctgacccac ccaccccatc
      121 gatcggagac gaggccgggc ctacgggccc ggcctcgctc gcgtcaggac ctccggcatc
      181 gacggcggcg gtccccggcg gcgcatcgcc gcgcgctcgg ggctccatcg cccgggtgca
      241 gcgtggacct ggccaccgcg ggaccaccgg tgctcgtcgc ccccgccctc cgcgacgggc
      301 gcgaccgtcg ttccacggcc cggtcacctc ggtcccgctg ccaccgacct tctgacaaac
      361 cgtcaataga tcttccagca tccccgcggc agatccaggt gacctcgacc ctgaacccct
      421 agccgagccg cagggtttcc cacatggttt cccacacctg tggataaccc cgcggacgac
      481 ggtgaggccg agccgccaag agcggccgcc gagtcacgtg agcgcgacca tcgagcggag
      541 gtcgatggtc gccgctcgac tcaggcggtg ggcgactccg ggccagcggt catggcccgg
      601 tagatgcgct cgaggtcttc gaggctggcg aagtcgatcg tgaccttgcc gcgcttcgcc
      661 cccatcgaga ccttgacccg cgtgtcgaga cgatcggaga gcaggccttc gagctcgagc
      721 aagcccggcg ggcgcagccg gcgacccgcc acgcccgggg tcggcttggt cccgggctcg
      781 ggatcgtcgt cggcctccac ctcacccgaa cgggcccgga cggcctcctc gacctcccgg
      841 acgttcagct gctcggcgac ggctcggcgg gccagcgcct cctggaagga ccggtccggg
      901 gtgccgagca gggcgcgggc gtggccggca gagagctgac gatcggcgac gagcttctgg
      961 atgctgggcg ggagctggaa caaccgcagc atgttcgaga ccgctgaacg gctcttgccc
     1021 acgcgccggg ccacatcctc gtgggagagg ttgaactcct cgatgagctg ctggtaggcc
     1081 gccgcctcct ccatcgggtg gaggtcctcg cggtggaggt tctccaccag cgcctgctcg
     1141 agcgacgtgg tctcgtcgat cttgcggacg atcgcgggga tggtctgcaa gccggcacgc
     1201 ttggaggccc gccaccgccg ctcccccgcg atcagctcgt agcggtcgtg tcccgatgga
     1261 cggaccagga tgggctggag gacacccagc tcacggatcg aatcggtcag cgagaccagc
     1321 gcctcttcgt cgaactgcgc ccgaggctgg tgctggttcg gctcgatctg gctgacctgg
     1381 agctctcgca gggtcgcgtc gtcgctcggc gccgtgagat cgttggggat gagcgcgccg
     1441 aggcccttcc cgagtccgct acgccgggcc accgctgacc tcccttgcca gttcccggta
     1501 cgccaccgca ccacgagacg acggatcgaa cgccgtgatc ggctggccga acgacggggc
     1561 ttccgagaga cggacggtac gcgggatcat gatcttgcac accttgtcac cgaagtgggc
     1621 acggacctcc tgcgccacct gatccgacag cttggtccgg gcgtcgtaca tcaccaagac
     1681 gatggtcgag atgtccagca cggggttgag gttcttggtg accagaccga cgttgcgcat
     1741 cagctgaccc aggccctcga gcgcgtagta ctcgcactgg atcggcacga ggacctcgga
     1801 tgcagccgcc atcccgttga tggtcagaag gccgagcgac ggcggacagt cgatgagcac
     1861 gtagtcgtag tcatcccgca cctcgtgaag cgcctgcttg agccgcagct cccggctgaa
     1921 ggccggcacc agctcgatct cggcgccggc caggtccagg ctggccggtg ccacgaacag
     1981 gttcttgacc gaagaggcct cgacgcagtc ctcgatcgga acatcgctca ggatcacgtc
     2041 gtacatggag gcctggagcc cacggatgtt gatgcccaga ccagtgctgg cgtttccctg
     2101 aggatcgagg tcgaccacga ggacccgata ccccaggtcg gcaagcgcag cgcccaggtt
     2161 cacggtggtg gtggtcttcc cgacgccgcc cttctggttc gccacggcga ggatgcgggg
     2221 aaggggcggg cggcttcgcg gcgtcgctgc ggaagcctcg tcgcccgtcg gatccgactc
     2281 ga
//
LOCUS       DATMFS010000167         9230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_309217, whole genome shotgun sequence.
ACCESSION   DATMFS010000167 DATMFS010000000
VERSION     DATMFS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309217"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VNQ33_03075"
     CDS             <1..246
                     /locus_tag="VNQ33_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HWJ97114.1"
                     /translation="LTAERRREYVRIVKGMAEEGRIAVRNLRRSGRQELEAFQKDGEL
                     SEDELARAEKELDKVTHAAEAEIDAALAAKEQELLED"
     gene            330..1913
                     /locus_tag="VNQ33_03080"
     CDS             330..1913
                     /locus_tag="VNQ33_03080"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWJ97115.1"
                     /translation="MDDQRGSRSDDPKPTDAAEGVRIIGADEAAEAMERDDVAPRRTD
                     DELRYGDRPPAPPAGPRPALRFPLDASADPTRIERPPVEPAPDPVTGPVSLPHWTEPP
                     TGEVPAVLIGDEGPLMDEDEDDLDVWSSFATSTPRWRDADDDWGDDGFMEKLADESEV
                     RVGALGDVDAEPSHEEYFGFDDMEERVEERTGDPGVPTDPGPRWEVGDDDWSAAGDQP
                     EAVVEEAWGADEPWADDGDADEYVDEDIYAPAGGGGDRNMPMAVGVGVGLAAIGVIAF
                     VAGSAATTILVTLILGACAVEYFEVLRKVGWESARVVGLAAVVALPLAVHWRGLPAYA
                     LIAFLTVATILIWYVVGAGGRDPRVLEGTGATLLGVIWIGGLGSSATFMASMSKGISL
                     LLVAVLATVAYDVGGLFIGKAMGHRPLAAASPNKTVEGLLGGMAGAFLVPVILVGMLD
                     VGPWSGLGAGLALGIGAAVAAPLGDLCQSVLKRDLGVKDMGSLLPGHGGVLDRFDAML
                     FVLPAVLWLTLALGPFNVV"
     gene            1910..3121
                     /gene="dxr"
                     /locus_tag="VNQ33_03085"
     CDS             1910..3121
                     /gene="dxr"
                     /locus_tag="VNQ33_03085"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWJ97116.1"
                     /translation="MTTTVALAGSTGSIGTQTIEVVAAEPDRYEIVALGATGRNLDQL
                     ADQVAAVRPKVVAIADPSRADAARERLGGVEIRVGTEGLASLGAEADVCVNGVVGFAG
                     LEVTLATLLAGRRLALANKESLIAGGPVVQRARRTPGAELVPVDSEHGALHQCLRSRD
                     ESGPARVAALVLTASGGPFRGRGAADLAQVTVEDALAHPTWSMGPKITVDSSTLMNKG
                     LEVIEANELFGTPAGEHGVGIGFDEIDVVVHPQSVVHSMVTYTDGSTIAQLSMPSMCL
                     PIGYALAWPDRSAAPFGAIDWTTLSRLDFEPPDFGAFPCLGLAYAAGRAGGVAPAWLN
                     AANEEAVDAFLNGAIAWTDIASVCATVLDGHEAGVPADVEDVVEADRRARLAARSVMG
                     LAPPPQDPLDA"
     gene            3114..4556
                     /locus_tag="VNQ33_03090"
     CDS             3114..4556
                     /locus_tag="VNQ33_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWJ97117.1"
                     /translation="MPEPPPADTDTIEAPPRPAAPGGATRLASSVSSEGYGVDPESPG
                     VRFGWLRLGALLAVLVLVGFLNPQMLLVILALMLMIALHELGHYLTAKRAGMKVTEFF
                     LGFGPKIWSVRRGETEYGIKCIPAGAYVKIPGMVNIDEVAPEDEARTYRQQSFGARVS
                     VAVAGSAMHFMLALVLLYVSAVFIGQPADAFSTNPASPPPVVKQVHPGSGAAAAGLKP
                     GDEIRTIDGTATPTGTALRTVSAPHYGDTVPVTFLRDGRIQTADLTLGAYSFTLADGA
                     VAKTCGLGITMADPPDETVGPIQGLVKAPQQFGQIFAISVQGLGSFFSPSGISSFGSQ
                     VGSAHEDSKAAKESEADNSETCAEKAAAIAKDPAGPAVSASSTGENRILSIYGLVRIG
                     SSVDVVSLVTLFALINIFIGIFNLVPLLPFDGGHVAIAVYENIQERRLHRRRYFADVT
                     RLLPLTYGVVLVLGLLFVSSLYLDVVNPIG"
     gene            4559..5806
                     /gene="ispG"
                     /locus_tag="VNQ33_03095"
                     /gene_synonym="gcpE"
     CDS             4559..5806
                     /gene="ispG"
                     /locus_tag="VNQ33_03095"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWJ97118.1"
                     /translation="MDDGSITLAARRPTRQIVLAHPTHPVAVGGGAPVSVQSMTTTKT
                     ADVDGTLQQVYALYGAGADIVRCTCNDADAAAGLAHIVPRSPVPIVADVHNNYRMALA
                     ALEAGVHCLRLNPGNIKRPEHIKLIAQEAKDRGVPIRIGVNGGSLDERLYQKYGGKVT
                     PEAMVESAQMELAYFDEVGFDQVKISVKASSVPLMVEAYRQLSEATDHPLHLGVTEAG
                     PPPAGVVKATAGIAALLAEGIGDTIRYSLTADPVEEARAGRVLLEAMGLRERKGLDLI
                     ACPSCGRAEVDVIKVANDAQAALDGMDFPIQVAVMGCVVNGPGEARDADLGIAAGRKK
                     GHLFVKGQVVAVVPEDEMVDALVHWAGTIGEEGIEGALTYADANAQAEADADRAALLD
                     EQGADANASEAKVELIRKNLADR"
     gene            complement(5833..6366)
                     /locus_tag="VNQ33_03100"
     CDS             complement(5833..6366)
                     /locus_tag="VNQ33_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWJ97119.1"
                     /translation="MSATDDPVGRSSEDRAPDGAPAPGTSGRRGDLDGDGRLDARERF
                     GVLKEAAIQAEYATGRHEDTEEEAKRHVALRIGTIIVGFVVLFGGLAMMILPGPGIIG
                     IIAGLGILSRELTWAERMLEYAKEKARVDELKEQPTWVKAGAWAITIGAVAASAWYLF
                     LADPKPELTEALPWNWD"
     gene            complement(6484..6753)
                     /locus_tag="VNQ33_03105"
     CDS             complement(6484..6753)
                     /locus_tag="VNQ33_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWJ97120.1"
                     /translation="MPLRPRLPRAPPRRAGPPRARPLIETRCGLQGPAPYGGPPVRAT
                     WLADRAGSLVDEHPGAYKDIHKVMRDQADLVTVRAELRQVVNDKG"
     gene            complement(6684..6977)
                     /locus_tag="VNQ33_03110"
     CDS             complement(6684..6977)
                     /locus_tag="VNQ33_03110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97121.1"
                     /translation="MSIEITHEPQGDRGRYHLLVDGAEAGEIDYRMDGEVRVFVHTGV
                     RDAYEGQGLAGKLAKHVLDDARANGIRIGATCPYVRGYLERHPEEQDLLAHAL"
     gene            complement(7000..7293)
                     /locus_tag="VNQ33_03115"
     CDS             complement(7000..7293)
                     /locus_tag="VNQ33_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97122.1"
                     /translation="MDIEIDDERHGALGQYHLFVDGTEVGELDYRDGDGRRVFTHTGV
                     RPAFEGRGLAARLVRRGLDDARAEQRKVIPQCSYVAAYLAQHPGDADLLATTE"
     gene            7400..7969
                     /locus_tag="VNQ33_03120"
     CDS             7400..7969
                     /locus_tag="VNQ33_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97123.1"
                     /translation="MLRTPFRTVALVLLAGTLVAACGASGGDDAAGTTSASKSGSTTS
                     AAPTTEAADATETTEADDPAETAEADAPGDDICVPLKVLSDFDIESSQLVNGGDWAAT
                     QAYFVEHTDDVLAAYDEAIAMDSEVEDDLKALRAVTETTAETAADSTGLMDFSAKLLD
                     QPGIMEAGAAGFALNTFAEDNCGFSTGGN"
     gene            8053..>9230
                     /gene="proS"
                     /locus_tag="VNQ33_03125"
     CDS             8053..>9230
                     /gene="proS"
                     /locus_tag="VNQ33_03125"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWJ97124.1"
                     /translation="MAKPPVLTPQADDFPRWYQDVLAKAELADNGPVRGTMVIRPYGY
                     GLWERMVEEVDARIKDAGAENAYFPLFIPESYLTREAEHVEGFSPELAVVTHAGGKEL
                     EEPVVVRPTSETVIGEFMAKWVNSHRDLPLLLNQWANVVRWELRTRIFLRTSEFLWQE
                     GHTAHATEADASAYALRILEDVYRDFMVEVLAMPVHIGRKTKQERFAGATNTLACEAM
                     MGDGKALQMGTSHELGQNFAKAFEIKYQSAEGVEELCWTTSWGVSTRMMGGLIMCHGD
                     DKGLVIPPRLASNQVVVLVIKDEDGVGAAAQALVDEIKAEGIRVKLDDRTDTSFGRRA
                     TAWELKGVPVRVEVGPRDLVNGEVTVVRRDTGAKTQVGIAGLATDLPAVLDGIQADLL
                     A"
BASE COUNT         1342 a         3326 c         3273 g         1289 t
ORIGIN      
        1 ctcaccgccg aacgccgtcg ggagtacgtg cggatcgtca agggcatggc cgaggagggc
       61 cggatcgccg tgcgcaacct gcgccgctcg ggccgccagg agctggaggc cttccagaag
      121 gacggcgagc tgtccgaaga cgagctcgct cgggccgaga aggagctcga caaggtcacg
      181 cacgccgcgg aggcagagat cgatgctgcg ctcgcagcca aggagcagga gctgctcgaa
      241 gactgaccag ggtgcggccg gtccgcacca cacccccgag ccggcggcgg tcgctgcggt
      301 tcggaccagg cgcaacccca ggaggtgcca tggacgacca acgaggatct cgatcagacg
      361 acccgaagcc gaccgacgcg gcagagggcg tccgcatcat cggcgccgac gaggccgccg
      421 aggcaatgga gcgcgacgac gtcgctcccc gacggaccga tgacgaactg cgctacggcg
      481 accgcccccc ggcgccgccg gcgggccccc gtcccgcgct gcggttcccg ctcgatgcca
      541 gcgcggatcc gacccgcatc gaacggccgc cggtcgaacc ggccccggac ccggtcaccg
      601 ggcccgtgtc gctcccgcac tggaccgagc cgcccaccgg cgaggtcccg gccgtgctga
      661 tcggcgacga gggcccgctg atggacgagg acgaagacga cctcgacgtg tggtcgtcct
      721 tcgcgacgtc caccccgcgc tggcgcgacg ccgacgacga ctggggcgac gacgggttca
      781 tggagaagct cgccgacgag agcgaggtgc gcgtgggcgc cctcggcgat gtcgacgccg
      841 aacccagcca cgaggagtac ttcgggttcg acgacatgga ggagcgggtc gaggagcgca
      901 ccggcgatcc cggcgtgccc accgatcccg gtccccgctg ggaggtcggc gacgacgact
      961 ggagcgcggc cggcgaccag cccgaggcgg tcgtcgagga ggcctggggc gccgatgagc
     1021 cgtgggctga cgacggcgac gcggacgagt acgtcgacga ggacatctac gccccagcgg
     1081 gcggcggcgg cgaccgcaac atgccgatgg ctgtcggcgt cggggtcggc ctggccgcga
     1141 tcggcgtcat cgccttcgtc gccggatcgg ccgccaccac catcctcgtg accctcatcc
     1201 tcggcgcgtg cgccgtggag tacttcgagg tcctccgcaa ggtcggctgg gaatcggctc
     1261 gcgtggtcgg cctggccgcg gtcgtggcgc tcccgctcgc cgtccactgg cgcggtctcc
     1321 cggcgtacgc gctgatcgcc ttcttgacgg tggcgaccat cttgatctgg tacgtcgtcg
     1381 gtgccggcgg gcgcgacccg cgggtgctcg aaggcacggg ggccaccctg ctcggcgtga
     1441 tctggatcgg cggcctcggc tcgtcggcca cgttcatggc gtcgatgtcc aagggcatct
     1501 cgctcctgct cgtcgccgtc ttggccaccg tcgcctacga cgtcggcggc ttgttcatcg
     1561 gcaaggcgat gggccaccgg ccgcttgccg cggccagccc gaacaagacc gtcgaaggcc
     1621 tcctcggggg catggcgggc gcgttcctcg tgcccgtgat cctcgtgggc atgctcgacg
     1681 tgggtccgtg gtccggcctc ggcgccggcc tggcgctcgg catcggagca gcggtcgcgg
     1741 cgccgctcgg cgacctgtgc cagtccgtgc tgaagcgcga cctcggcgtc aaggacatgg
     1801 gcagcctgct gcccggccac ggcggcgtgc tggaccgctt cgacgccatg ttgttcgtcc
     1861 tgccggccgt gctctggctg acgctggccc tcggtccgtt caacgtggtg tgaccaccac
     1921 cgtcgccctc gccgggtcca ccggctccat cggcacccag accatcgagg tcgtggcggc
     1981 cgaacccgac cgctacgaga tcgtcgccct cggcgccacc ggacggaacc tcgatcagct
     2041 cgccgaccag gttgcggcgg tgcggccgaa ggtcgtcgcc atcgccgacc cgtcccgggc
     2101 cgatgcggcg cgcgagcgcc tcggcggcgt ggagatccgc gtcggcaccg aaggcctcgc
     2161 cagcctcggc gccgaggccg atgtctgcgt gaacggcgtg gtgggcttcg ccggtctcga
     2221 ggtcaccctc gccacgctgc tcgccggcag gcgcctggcg ctggccaaca aggagtcgct
     2281 catcgccggt ggcccggtcg tgcagcgggc gcgccgcacg cccggggccg aactggtgcc
     2341 cgtcgacagc gagcacggtg cgctccacca gtgcctgcgc tcccgggacg agtccggtcc
     2401 ggcgcgcgtc gccgcgctcg tcttgacggc cagcggcggc ccgttccgcg ggcgcggcgc
     2461 cgccgacttg gcccaggtga ccgtcgagga cgccctggcc cacccgacct ggagcatggg
     2521 ccccaagatc acggtcgact cgtcgaccct gatgaacaag ggcctcgagg tcatcgaggc
     2581 caacgagctg ttcggcacac cggcggggga gcacggcgtc gggatcggct tcgacgagat
     2641 cgacgtcgtg gtccacccgc agtcggtcgt ccactcgatg gtcacctaca cggacggctc
     2701 caccatcgcc cagctctcga tgccctccat gtgcctgccc atcgggtacg ccctggcctg
     2761 gcccgaccgc tcggcggcgc cgttcggcgc catcgactgg acgacgctgt cccgcctcga
     2821 cttcgagccg ccggacttcg gcgccttccc gtgcctcggc ctcgcctacg ccgccgggcg
     2881 cgccggcggg gtggctcccg cctggctcaa cgccgccaac gaggaagcgg tcgacgcgtt
     2941 cttgaacggc gccatcgcct ggaccgacat cgcgtcagtg tgcgccacgg tcctcgatgg
     3001 tcacgaggca ggcgttcctg ccgatgtgga ggacgtggtc gaagccgacc gccgggcgcg
     3061 gctcgcagcc cgtagcgtga tggggctcgc acccccgccc caggaccctc tcgatgcctg
     3121 aaccgccccc cgccgacacc gacacgatcg aggccccgcc gcgccccgcc gcgccgggcg
     3181 gtgcgacccg tctggccagc tccgtctcct ccgaggggta cggcgtcgac ccggagtcgc
     3241 cgggcgtccg cttcggctgg ctgcgcctcg gtgcgctcct cgccgtgctg gtgctggtcg
     3301 ggttcttgaa cccgcagatg ctcctcgtga tcctcgccct catgttgatg atcgccctgc
     3361 acgagctggg ccactacctc acagccaagc gggcgggcat gaaggtcacc gagttcttct
     3421 tgggcttcgg accgaagatc tggtccgtgc gccgcggcga gaccgagtac gggatcaagt
     3481 gcatcccggc cggggcctac gtgaagatcc ccgggatggt caacatcgac gaggtcgcgc
     3541 cagaggacga ggcgcggacc taccggcagc agagcttcgg tgcacgcgtg tcggtcgccg
     3601 tcgccggctc ggcgatgcac ttcatgttgg ccctcgtgct gctctacgtg agcgcggtgt
     3661 tcatcgggca accggccgac gcgttctcga cgaacccggc cagcccgcca ccggtggtga
     3721 agcaggtcca cccgggctcg ggcgcggcgg cggcgggcct gaagcccggc gacgagatcc
     3781 gcaccatcga cgggacggcg acgccgaccg gcaccgcgct gcgcacggtc tccgcgccgc
     3841 actacggcga caccgtgccg gtgaccttcc tccgcgacgg caggatccag acggctgacc
     3901 tcacgctcgg cgcctactcc ttcacgctcg ccgacggcgc cgtggccaag acgtgcggcc
     3961 tgggcatcac gatggccgat ccgccggacg agaccgtcgg tcccatccag ggactggtca
     4021 aggccccgca gcagttcggt cagatcttcg ccatctcggt ccagggcctc ggcagcttct
     4081 tcagcccgtc gggcatctcg tccttcggct cgcaggtcgg gagcgcccac gaggacagca
     4141 aggcggccaa ggagtccgag gccgacaaca gcgagacgtg cgccgagaag gccgccgcca
     4201 tcgccaagga cccggcgggc ccggccgtct cggcctcgtc gaccggcgag aaccggatcc
     4261 tgtccatcta cggcctggtg cgcatcggct cgtcggtcga cgtggtctcg ctggtgacgc
     4321 tcttcgccct catcaacatc ttcatcggca tcttcaacct ggtcccgctg ctgcccttcg
     4381 atggcgggca cgtggccatc gccgtgtacg agaacatcca ggagcggcgc ttgcaccgcc
     4441 gtcggtactt cgccgacgtg acccggctgc tgcccctcac ctacggggtg gtcctcgtgc
     4501 tcggcctgct gttcgtgtcg agcctgtacc tcgacgtcgt caacccgatc ggctgatcgt
     4561 ggacgacggc tccatcaccc tggccgcccg gcgcccgacc cggcagatcg tcctggcgca
     4621 cccgacccac cccgtcgcgg tgggcggcgg tgcgccggtg tcggtccagt cgatgaccac
     4681 caccaagacg gccgacgtcg acggcaccct ccagcaggtg tacgccctct acggcgccgg
     4741 ggccgacatc gtccgctgca cctgcaacga cgccgacgcc gcggcgggcc tcgcccacat
     4801 cgtgcctcgc tcgccggtcc cgatcgtggc cgacgtccac aacaactacc ggatggcgct
     4861 ggccgcgctg gaggccgggg tccactgcct gcgcctgaac cccgggaaca tcaagcggcc
     4921 cgagcacatc aagctcatcg cccaggaggc gaaggaccgg ggcgtcccga tccgcatcgg
     4981 ggtcaacggc ggctcgctcg acgagcggct gtaccagaag tacggcggga aggtcacccc
     5041 ggaggcgatg gtcgagagcg cccagatgga gctggcttac ttcgacgagg tcgggttcga
     5101 ccaggtgaag atctcggtca aggcatcgag cgtgccgctg atggtcgagg cgtaccggca
     5161 gctgtccgag gcgacggacc accccctgca cctgggcgtc accgaagcgg gtccgccgcc
     5221 agcgggtgtg gtgaaggcca cggccggcat cgcggccctg ctggccgagg gcatcggcga
     5281 cacgatccgc tactcgctca ccgccgaccc cgtcgaggag gcgcgcgccg ggcgcgtgct
     5341 gctggaggcc atgggcctcc gtgagcgcaa ggggctcgac ctcatcgcct gcccgtcgtg
     5401 cggacgggcc gaggtcgacg tgatcaaggt ggcgaacgac gcccaggcgg cgctcgacgg
     5461 catggacttc ccgatccagg tcgccgtgat gggctgcgtg gtgaacggcc cgggcgaggc
     5521 gcgcgacgcc gatctcggca tcgccgccgg tcgcaagaag ggccatctgt tcgtgaaggg
     5581 ccaggtcgtc gccgtcgtgc ccgaagacga gatggtcgac gcactggtcc actgggcggg
     5641 caccatcggc gaggagggca tcgaaggcgc cctcacctac gccgatgcca acgcccaggc
     5701 cgaggccgac gccgaccgcg ccgccctcct cgacgagcag ggcgccgacg ccaacgcgtc
     5761 ggaggccaag gtcgagctga tccgcaagaa cctcgccgac cgctgacccg cctccgggtc
     5821 gtcagagaga ggtcagtccc agttccaggg cagcgcctcg gtgagctcgg gcttcgggtc
     5881 ggccaggaac aggtaccagg ccgacgcggc gacggcgccg atcgtgatcg cccaggcacc
     5941 ggccttcacc cacgtgggct gctccttgag ctcgtcgacc cgtgccttct ccttggcgta
     6001 ctcgagcatc cgctcggccc aggtcagctc tcgggagagg atgcccaggc cggcgatgat
     6061 gccgatgatg ccgggacccg ggaggatcat catggcgagg ccgccgaaca gcacgacgaa
     6121 gccgacgatg atcgtgccga tgcggagcgc gacgtggcgc ttggcctcct cctcggtgtc
     6181 ctcgtggcgg ccggtggcgt actcggcctg gatcgccgcc tccttcagca cgccgaagcg
     6241 ctcacgagcg tcgaggcggc cgtcgccgtc caggtcgccg cgccgcccgc tggtcccggg
     6301 agcaggagcg ccgtcagggg ctcggtcctc ggaggagcgg ccgaccgggt catccgttgc
     6361 tgacatcgac ccagtatcgc ggtcgaggcc gacggcgtca gtagggcatt cccccgaacc
     6421 gaccgatcgg gaccacctgc tcccgatccg gggaccggga gcaggccgcc atcgagcgac
     6481 ggctcagccc ttgtcgttga cgacctggcg cagctcggcc cggaccgtca cgaggtcggc
     6541 ctggtcccgc atcaccttgt ggatgtcctt gtaggcaccg gggtgctcgt ccaccaggga
     6601 gccggcgcgg tcggcgagcc acgttgcccg aaccggcggg ccaccgtacg gagccggccc
     6661 ttgcaggccg cagcgagttt cgatcagagg gcgtgcgcga ggaggtcctg ctcttcgggg
     6721 tggcgctcga ggtagccgcg gacgtagggg caggtggcgc cgatcctgat gccgttcgcc
     6781 cgggcgtcgt cgaggacgtg cttggcgagc ttgccggcca ggccctggcc ctcgtaggcg
     6841 tcgcgcacgc cggtgtggac gaacacccgg acctcgccgt ccatgcggta gtcgatctcg
     6901 ccggcttcgg cgccgtcgac caagaggtgg tagcggccgc ggtcgccctg gggctcgtgg
     6961 gtgatctcga tgctcatggc ccccaggatg ccgggtggat cactcggtcg tcgcgaggag
     7021 gtcggcgtcg ccggggtgct gggcgaggta ggcggcgacg tacgagcact gggggatgac
     7081 cttccgctgc tcggcccggg catcatcgag gccgcggcgc acgagccggg cggccaggcc
     7141 ccggccctcg aaggccggac gcacgccggt gtgggtgaac acccggcggc cgtcgccgtc
     7201 gcggtagtcg agctcgccga cctcggtgcc gtccacgaac aggtggtact gcccgagcgc
     7261 gccgtgccgt tcgtcgtcga tctcgatgtc catggcccca ggatgcccgg atgcgacccc
     7321 gacgagacgc ctccaggttg ggaccgttct gggacgccga tctgcggccc ggtaccggcg
     7381 aggcccgtac gatcgtcgca tgctccgcac gccgtttcgc accgtcgccc tcgtgctgct
     7441 cgccggcacc ctcgtcgccg catgcggcgc gtcgggcggc gacgacgcgg ccggcaccac
     7501 cagcgcatcc aagtccggat ccacgacgtc ggcggcaccc accaccgagg cggccgacgc
     7561 gaccgagacc acggaggccg acgatccggc ggagacggcc gaggccgacg cgcccggcga
     7621 cgacatctgc gtgccgttga aggtgctgag cgacttcgac atcgagtcct cccagctggt
     7681 gaacggcggg gactgggccg ccacgcaggc gtacttcgtc gagcacaccg acgacgtcct
     7741 cgccgcctac gacgaggcga tcgccatgga cagcgaggtc gaggacgacc tgaaggccct
     7801 ccgagcggtc accgagacca ccgcggagac ggcagccgac tcgaccggtc tcatggactt
     7861 cagcgccaag ctcctcgacc agccgggcat catggaggca ggggccgcgg gcttcgccct
     7921 caacaccttc gccgaggaca actgcgggtt ctcgacgggc ggcaactgac ggcgagccgg
     7981 cgccgcccgg ctcggcaccg tcgcggatcc gaggtcggag cgggggagcg gcggccggta
     8041 cggtggcccg ccatggccaa gccccccgtc ctcactcccc aggctgacga cttcccccgc
     8101 tggtaccaag acgtgctggc gaaggcggag ctggcggaca acgggccggt gcgcggcacg
     8161 atggtcatcc ggccctacgg ctacggcctg tgggagcgca tggtcgagga ggtcgacgcc
     8221 cgcatcaaag acgccggcgc cgagaacgcc tacttcccgc tgttcatccc cgagagctac
     8281 ctgacccggg aggccgagca cgtcgagggc ttcagccccg agctggcggt ggtgacccac
     8341 gccggcggca aggagctgga ggagcccgtg gtggtccgcc ccacgagcga gacggtcatc
     8401 ggcgagttca tggccaagtg ggtcaacagc caccgcgacc tgccgctgct gctcaaccag
     8461 tgggccaacg tcgtgcgctg ggagctgcgg acccgcatct tcctgcggac ctcggagttc
     8521 ctctggcagg aggggcacac ggcgcacgcc accgaggccg acgccagcgc ctacgcgctc
     8581 cggatcctcg aggacgtgta ccgcgacttc atggtcgagg tgctggccat gcccgtgcac
     8641 atcggccgca agaccaagca ggagcgcttc gccggcgcca ccaacacgct cgcctgcgag
     8701 gcgatgatgg gcgacggcaa ggcgctccag atgggcacca gccacgagct gggccagaac
     8761 ttcgccaagg cgttcgagat caagtaccag agcgccgagg gtgtcgagga gctgtgctgg
     8821 accaccagct ggggcgtgtc gacccgcatg atgggcgggc tcatcatgtg ccacggcgac
     8881 gacaagggcc tggtgatccc gccccgtctg gcttcgaacc aggtcgtggt cctcgtgatc
     8941 aaagacgaag acggcgtggg cgcggccgcg caggcgctgg tggacgagat caaggccgag
     9001 ggcatccggg tgaagctcga cgaccgcacc gacacctcgt tcggccgccg cgccacggcg
     9061 tgggagctga agggcgtgcc cgtgcgggtc gaggtgggcc cgcgcgacct cgtgaacggc
     9121 gaggtcacgg tcgtgcgccg tgacacggga gcgaagacgc aggtcggcat cgccggcttg
     9181 gcgacggacc tgcccgcggt gctcgacggc atccaggccg acctcctggc
//
LOCUS       DATMFS010000168         5278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_332693, whole genome shotgun sequence.
ACCESSION   DATMFS010000168 DATMFS010000000
VERSION     DATMFS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332693"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="thrC"
                     /locus_tag="VNQ33_03130"
     CDS             <1..1039
                     /gene="thrC"
                     /locus_tag="VNQ33_03130"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWJ97125.1"
                     /translation="VGRLFDHELTRRGERATIVVATSGDTGSAAIEACRGRSNLDIVV
                     LHPAGRVSDVQRKQMTTVREPNVHNVAVEGTFDDCQDLVKQMFADAAFRDAVGLSAMN
                     SINWARVMAQVVYYVTAAQAVGAGDPVSYSVPTGNFGNVFAGWVARRTGTPIGQLVVG
                     SNRNDILTRWLSSGVMEKTGVHPTISPSMDIQVSSNAERLLFELYERNALLLVDRMAE
                     FRDEGRVETGDTRLASVTEVFDGASFDDDATRAEIRRTYEATGQILDPHTAVGVAAAR
                     AARRDPSLPMICLATAHPAKFPDAVEAAIGIRPDLPPHLADLFDREESYETMPADLAV
                     LQHHVKALTGH"
     gene            1075..1440
                     /locus_tag="VNQ33_03135"
     CDS             1075..1440
                     /locus_tag="VNQ33_03135"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97126.1"
                     /translation="MEKVDGIGGFFFTAQDPAALGRWYAEHLGIAEVPTAYDAEVWTQ
                     SAGETVFAPFPADAGDETPVGPSGWGINFRVTDLEAMAAQLRGAGIEVEVDPTEYPNG
                     WFAQLSDPEGNAIQLWQPT"
     gene            1534..1776
                     /locus_tag="VNQ33_03140"
     CDS             1534..1776
                     /locus_tag="VNQ33_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWJ97127.1"
                     /translation="MAATMTSKGQITVPKAVRDALGLSEGDRVYFRVEGDRAFLARTP
                     DFLDLAGTFAVPAARRGAEWSNVVSAARADRAARIG"
     gene            1773..2168
                     /locus_tag="VNQ33_03145"
     CDS             1773..2168
                     /locus_tag="VNQ33_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ97128.1"
                     /translation="MTAFVDTNVLIRHLTGDPADQAAAATAFLAAADQLLLTDLITAE
                     TAYVLGSYYEAPREQVAAALRSLVSMPSVSTIDPALLLRAIQVYELDRLDFAEAYLVA
                     CAEANGVPRIASFDRAIDRVASVERVEPA"
     gene            2235..2447
                     /locus_tag="VNQ33_03150"
     CDS             2235..2447
                     /locus_tag="VNQ33_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWJ97129.1"
                     /translation="MARTNIDIDEDACAAVMRRYGCKTKREAVNLALRQVAREMSLDE
                     ALAMRGTGWDGDLDEIRSGMGAPELW"
     gene            2444..2830
                     /locus_tag="VNQ33_03155"
     CDS             2444..2830
                     /locus_tag="VNQ33_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HWJ97130.1"
                     /translation="MTLIDSSAWIEFLRDGNVATADAVEALLRSGGALTEPVVMEVLA
                     GARSGRHGEQIRTLLGTVDLLPVTAEDWATAALLYRMCRVNGATIRSMVDCLIASVAI
                     RNDVPVLAEDRDFEALAAHTPLRLAA"
     gene            complement(2897..>5278)
                     /locus_tag="VNQ33_03160"
     CDS             complement(2897..>5278)
                     /locus_tag="VNQ33_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWJ97131.1"
                     /translation="LSGGQRQRVALARAVLADPKILLLDDATSAVDSRIEAEIHQTLR
                     EVLAGRTTLLVAHRRSTLLLADRIAVLDAGRVVDVGTHEQLEGRCSLYRLLLTGPGDE
                     LAADPDNLAEPPAEDVTVDGITPALWIRTGDEEAAAGQALAATGKRDTGSLGVGIGGP
                     GGGPGGGAMGMLGGLPASPELLARVDALRPANDEPDQPLDDARDEDRDFQFTRFIRPW
                     RRQLAVGFGLVALDAVASLAGPLLIRHGIDAGIDADPQSMRAVWMASLAYLFVVTADF
                     FVMTWETFITGRTAERSLWSLRVKVFSHLQRLGLDYYDREMGGRIMTRMTSDIEALTS
                     LLQTGLVTALVALLTCVGVGVALVVMDWRLALMAMVVMPPLVAATVWFRRRSGAAYDD
                     ARDKVAVVNADFQESLTDVRVAQAFTREDQNTKRFAGRSRDYLGARLRAQQLVAIYFP
                     FVEFLSGVAGAIVLAGGAALVRDDAMATGTLIAFVLYLDLFFSPIQQLSQVFDTYQQA
                     RVALVRIGEVLGEPSSIPEADDPVHPGQLTGRIELQDVRFAYASTGLEVLHGVDLTIE
                     AGETVAIVGETGAGKSTVEKLVARYYDVSSGSVRIDGTDIRDLDLGEYRRQLGVVPQE
                     AFLFAGTIRDNLAYGRPDATDAEVEDAARSVGAHDVIASLPGGYLHVVGEQGRSLSAG
                     QRQLLALARAQVVDPKILLLDEATANLDLASEARVSEAMGMVTSGRTALVIAHRLSTA
                     VTADKIVVMDDGLVAEVGTHADLLAADGTYARLWSAFNGEAPPDPEPAVAAAD"
BASE COUNT          760 a         1811 c         1940 g          767 t
ORIGIN      
        1 cgtcggccgg ctcttcgacc acgagctgac ccggcgcggc gagcgggcga ccatcgtcgt
       61 cgccacctcc ggcgacacgg ggtcggctgc catcgaggcg tgccgcgggc ggtcgaacct
      121 ggacatcgtg gtcttgcacc ccgccgggcg ggtgagcgac gtccagcgca agcagatgac
      181 cacggtccgc gagcccaacg tccacaacgt ggccgtcgag ggcacgttcg acgactgcca
      241 ggacctggtg aagcagatgt tcgccgacgc cgccttccgc gacgccgtgg gcctctccgc
      301 catgaactcc atcaactggg ctcgggtgat ggcgcaggtc gtctactacg tgaccgccgc
      361 ccaggccgtc ggcgccggcg acccggtctc gtactcggtg cccaccggca acttcggcaa
      421 cgtgttcgcc gggtgggtcg cccgtcgcac cggcacgccc atcggccagc tggtggtcgg
      481 gtccaaccgc aacgacatcc tcacccggtg gctgtcctcc ggcgtgatgg agaagaccgg
      541 cgtccacccg accatcagcc cgagcatgga catccaggtg tcgtccaacg ccgagcgcct
      601 cctgttcgag ctgtacgagc gcaacgcgct gctgctggtc gaccgcatgg ccgagttccg
      661 cgacgagggc cgcgtcgaga ccggcgacac ccgcctcgcc tcggtcaccg aggtgttcga
      721 cggcgcctcg ttcgacgacg acgccacccg cgccgagatc cgccgcacct acgaggccac
      781 cggccagatc ctcgacccgc acaccgcggt gggcgtcgcc gccgcccggg ccgcccggcg
      841 ggacccgtcg ctgccgatga tctgcctggc caccgcccac ccggccaagt tccccgacgc
      901 cgtcgaagct gccatcggca tccgccccga cctcccgccc cacctggcgg acctcttcga
      961 ccgcgaggag tcctacgaga cgatgcccgc cgatctggcg gtcctccagc accacgtcaa
     1021 ggccctcacc ggccactgac cgcgatcggg ctcggggtgg gcgagactcc gggcatggaa
     1081 aaggtcgacg ggatcggtgg gttcttcttc acggcgcagg atccggcggc gctcgggcgc
     1141 tggtacgccg agcacctcgg gatcgccgag gtgccgacgg cctacgacgc cgaggtgtgg
     1201 acccagtcgg cgggcgagac cgtgttcgcc ccgttcccgg ccgatgccgg cgacgagacg
     1261 ccggtgggcc ccagcgggtg gggcatcaac ttccgcgtga cggacctcga agcgatggcg
     1321 gcccagctgc gtggggccgg catcgaagtc gaggtcgacc cgaccgagta ccccaacggc
     1381 tggttcgccc agctcagcga ccccgaaggc aacgccatcc agctctggca gccgacctga
     1441 gccgggcggc cgctaccggt gcggcgttgt ggagggctga acgtcgtgcc agaatccttc
     1501 ttgttctgaa aaagtaagga gaaggttgtg gatgtggcag cgaccatgac gtcgaagggg
     1561 cagatcacgg tgcccaaggc ggtccgcgac gcactcggac tgtctgaggg tgaccgggtg
     1621 tacttccggg tcgagggcga tcgggccttc ctcgcccgga cgcccgactt cctcgatctc
     1681 gcggggacct tcgccgtgcc agcggcacgg cggggtgccg agtggagcaa cgtcgtgtcg
     1741 gccgcgcggg ctgatcgtgc cgcgcgaatc gggtgaccgc cttcgtcgac accaacgtcc
     1801 tcatccggca cctgacgggt gaccccgccg atcaggccgc ggctgcaacc gccttcttgg
     1861 cggcagcgga tcagctgctg ctcacggatc tgatcaccgc ggagaccgcg tacgtgctgg
     1921 gctcgtacta cgaggcaccg cgggagcagg ttgcggcagc cctccgctcg ctcgtgtcga
     1981 tgccgtctgt gtctacgatc gatcccgcgc tcctgctccg ggcgatccag gtgtacgagc
     2041 tcgatcggct cgacttcgcc gaggcatacc tcgtcgcctg cgccgaagcc aacggcgtgc
     2101 cccggatcgc ctcgttcgat cgggcgatcg accgggtcgc ttcggtcgag cgggtggagc
     2161 cggcctgacc ggttcgtctg gcagcaggtc cgccgtccgg ccatgttggt gtatcgcagg
     2221 tgtatcatga gtgtatggct cggaccaaca ttgacatcga tgaggacgcg tgtgccgcgg
     2281 tgatgcgcag atacggctgc aagaccaagc gcgaggcggt caacctcgcg ctgcggcagg
     2341 tcgcccggga gatgtcgctc gatgaggccc tggcgatgag gggcaccggc tgggacggtg
     2401 atctcgacga gatccgctcg gggatgggtg cgcccgagct gtggtgaccc tcatcgactc
     2461 gtcggcctgg atcgagttcc tgcgcgacgg gaacgtggcc accgccgacg cggtcgaagc
     2521 cttgttgcgg agtggcggcg cgctgaccga gccggtcgtg atggaggtcc tcgctggcgc
     2581 tcgttcaggc cggcacggcg agcagatccg gacgctgctc ggcacggtcg accttctgcc
     2641 cgtgactgcc gaggactggg caacggccgc gttgctgtac cggatgtgcc gggtcaacgg
     2701 ggcgacgatc cgctcgatgg tcgactgcct gatcgcttcg gtcgccatcc gcaacgacgt
     2761 tccggtgctc gccgaggacc gggacttcga ggcgctcgcc gcccacacgc cgctccggct
     2821 cgctgcttga cgaccgaaac ctccggacac cgcctccctc ggggagtgag gcggtgtccg
     2881 cccgcctgag acctgttcag tcggcggcgg cgacggcagg ttcggggtca ggcggcgcct
     2941 cgccgttgaa ggcggaccag aggcgggcgt aggtgccgtc ggcggcgagg aggtcggcgt
     3001 gggtgccgac ctcggcgacg aggccgtcgt ccatcaccac gatcttgtcg gcggtgaccg
     3061 cggtggacag gcggtgggcg atgaccagcg ccgtgcgccc ggaggtgacc attcccatgg
     3121 cttcgctgac gcgtgcctcg ctggccaggt ccaggttcgc cgtggcctca tcgagcagga
     3181 ggatcttcgg gtccaccacc tgggcccggg ccagggccag gagctggcgc tggccggccg
     3241 acagcgagcg gccttgctcg ccgacgacgt ggaggtaccc gccggggagg ctggcgatca
     3301 cgtcgtgggc acccacggat cgggcggcgt cttccacctc ggcgtcggtg gcgtcggggc
     3361 ggccgtaggc caggttgtcg cggatggtgc ccgcgaacaa gaacgcctcc tgcggcacga
     3421 cgccgagctg gcggcggtac tcgccgaggt cgaggtcgcg gatgtcggtg ccgtcgatcc
     3481 gcaccgaccc gctgctcacg tcgtagtagc gggccaccag cttctcgacg gtggacttgc
     3541 cggcaccggt ctccccgacg atcgcgacgg tctcgcccgc ctcgatggtc aggtcgacgc
     3601 cgtgcagcac ctcgagtccg gtcgaggcgt aggcgaaccg cacgtcctgg agctcgatgc
     3661 ggccggtgag ctggcccggg tgcacggggt cgtcggcctc ggggatcgac gacggctcgc
     3721 ccagcacctc gccgatgcgc acgagcgcca cccgagcctg ctggtaggtg tcgaacacct
     3781 gcgacagctg ctggatcggg gagaagaaca ggtcgaggta gagcacgaag gcgatcagcg
     3841 tgccggtggc catggcgtcg tcgcgcacga gcgcggcacc gccggcgagg acgatggccc
     3901 cggcgacacc ggagaggaac tcgacgaacg ggaagtagat cgccaccagc tgctgggcgc
     3961 gcaggcgggc cccgaggtag tcgcgcgacc gcccggcgaa gcgcttcgtg ttctggtcct
     4021 cccgggtgaa ggcctgggcg acgcggacgt cggtgaggct ctcctggaag tcggcgttga
     4081 ccacggccac cttgtcgcgg gcgtcgtcgt aggccgcacc cgaccggcgc cggaaccaga
     4141 cggtggcggc caccaggggc ggcatcacga ccatcgccat gagggcgagg cgccagtcca
     4201 tgaccacgag ggccacgccg acgccgacgc aggtgagcag cgcgacgagc gcggtgacca
     4261 gcccggtctg gagcagcgag gtgagcgcct cgatgtcgga ggtcatgcgg gtcatgatcc
     4321 gcccgcccat ctcccggtcg tagtagtcga ggcccagccg ctggaggtgc gagaacacct
     4381 tgacccgcag cgaccacagc gatcgttcgg cggtgcggcc ggtgatgaac gtctcccacg
     4441 tcatgacgaa gaagtcggcg gtgaccacga agaggtaggc cagtgacgcc atccacacgg
     4501 cgcgcatgct ctgcgggtcg gcgtcgatgc cggcgtcgat gccgtggcgg atgagcagcg
     4561 gaccggcgag cgaggcgacg gcgtcgagcg ccaccaggcc gaaacccacg gcgagctggc
     4621 ggcgccacgg ccggatgaac cgggtgaact ggaagtcccg gtcttcgtcc cgggcgtcgt
     4681 cgagcggctg gtcgggctcg tcgttcgcgg ggcgcagggc gtcgacgcgg gccaggagct
     4741 cggggctggc gggcaggccg ccgagcatgc ccatggcgcc gccgccgggg ccgccgcccg
     4801 gtccgccgat gcccaccccg agcgatccgg tgtcgcgctt gccggtggcg gcgagcgcct
     4861 ggccggcggc ggcctcctcg tcgcccgtgc ggatccagag cgctggggtg atgccgtcga
     4921 cggtcacgtc ctcggcgggc ggttcggcca ggttgtcggg gtcggcggcc agctcgtcgc
     4981 cgggtccggt gagcagcagc cggtagaggc tgcagcggcc ttcgagctgc tcgtgcgtgc
     5041 cgacgtcgac cacgcggccg gcgtcgagca cggcgatgcg atcagcgagg agcagggtgg
     5101 agcggcggtg ggccaccagc agggtggtgc gcccggcgag cacctcgcgg agggtctggt
     5161 ggatctcggc ctcgatgcgg ctgtccactg ccgaggtggc gtcgtcgagc agcaggatct
     5221 tcggatcggc gagcacggcc cgggccaggg cgacgcgctg gcgctgcccg cccgacag
//
LOCUS       DATMFS010000169         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193448, whole genome shotgun sequence.
ACCESSION   DATMFS010000169 DATMFS010000000
VERSION     DATMFS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193448"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="VNQ33_03165"
     CDS             complement(<1..1735)
                     /locus_tag="VNQ33_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97132.1"
                     /translation="MARRLGRVQGTLAVLTALGITAVVPVVAGTPVAAAPERTAAPAA
                     APPSGQVAAQPANARWANQIFERHAGAASPADVTALAAALDRSTNPSATIRAALLKRT
                     AVEPAVGQKFQDLLERSADPSGLGYWSTRVSDGRLSYEDLVVTLTISNEAWTKAGATP
                     EGYTHWVYEHVLGRGSDPSGQAYWESRLESGTSRDRFVRSFLRSGERGRAVVRNSYRG
                     ILLRNPDAGGLNSWVKKYTTSKAGELDIDVALLASHESRNAGCGYDPKYCLFPFPNSR
                     QTLQDSSTVTGQRIAFKPEWLPANNKGKHVDPTEWNRNDGFSPGQAIVLRVPGVDLEQ
                     SGLAGIDNIGASLDEDAPVIIWDQTSEERVPYWAELDSHAAAGSADQVLYIRPAVNYQ
                     AGHEIIVGIENLKDAAGDVLPAPAKFARERDWIVEHDIIDDFPDDVDLLGFGDPQYIV
                     QTTFDMWRMQQQGMAMEDLYLAWHFTVASTENTTGRMLHIRDDALAAVGSGAPAFTVG
                     KVTPNPREGVAKRIEGTFQVPKYLTGSGQPGDGFKNGANGVPVRNGTYTASYDCEIPS
                     SAADAPARPTVY"
     gene            1900..>2374
                     /locus_tag="VNQ33_03170"
     CDS             1900..>2374
                     /locus_tag="VNQ33_03170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWJ97133.1"
                     /translation="MSDGTLFDDAVGREPRSDTWTVPELSVHVGRLLVGAFPDDVWVE
                     GQIRNLNRSSNGHVYFQLAEPCPAGQQPNTQLSVTLLAPERTLVNTQIKRAGGGVRME
                     DGIEVRIQGRIRWYGPRGTLQLRMHGIDPDYTLGRLQADKDRTLAVLAAEGLLDRN"
BASE COUNT          321 a          843 c          841 g          369 t
ORIGIN      
        1 cgtacacggt gggccgggcg ggcgcgtcgg cggcgctgct ggggatctcg cagtcgtagg
       61 acgcggtgta ggtcccgttg cggacgggca caccgttggc cccgttcttg aacccgtcgc
      121 cgggctggcc gctcccggtc aggtacttgg gcacctggaa cgtgccctcg atgcgcttgg
      181 cgacgccctc tcgcgggttg ggcgtgacct tgccgacggt gaacgccggc gcgcccgagc
      241 cgacggcggc cagcgcgtcg tcgcggatgt ggagcatgcg gcccgtggtg ttctcggtcg
      301 aggcgacggt gaagtgccaa gccaggtaga ggtcctccat cgccatcccc tgctgctgca
      361 tccgccacat gtcgaacgtc gtctggacga tgtactgggg gtcgccgaag ccgagcaggt
      421 ccacgtcgtc ggggaagtcg tcgatgatgt cgtgctcgac gatccagtcg cgctcgcggg
      481 cgaacttggc cggagcgggc agcacgtcac ccgccgcgtc cttcaggttc tcgatgccga
      541 cgatgatctc gtggccagcc tggtagttca cggccggccg gatgtacagc acctggtcgg
      601 cagatccggc cgcggcgtgg gagtcgagct cggcccagta cggcacccgc tcctcgctgg
      661 tctggtccca gatgatcacg ggggcgtcct catcgaggct ggcgccgatg ttgtcgatgc
      721 cggcgaggcc cgactgctcg agatcgacgc cgggcacccg gagcacgatg gcctgaccgg
      781 ggctgaagcc gtcgttgcgg ttccactcgg tcgggtcgac gtgcttgccc ttgttgttgg
      841 ccgggagcca ctcgggcttg aaggcgatcc gctgtccggt caccgtgctg ctgtcctgca
      901 gcgtctggcg gctgttcggg aacgggaaca ggcagtactt cgggtcgtag ccgcagccgg
      961 cgttgcggga ctcgtggctg gccaggaggg cgacgtcgat gtcgagctcg cccgccttgg
     1021 aggtcgtgta cttcttcacc cagctgttga gaccaccggc gtcggggttg cgcaggagga
     1081 tgccccggta gctgttgcgc acgacggccc ggccccgctc gcccgagcgg aggaacgaac
     1141 gcacgaaccg gtcccgggac gtgccggact cgaggcggga ctcccagtac gcctggccgc
     1201 tcgggtccga gccccgaccg agcacgtgct cgtagaccca gtgcgtgtag ccctccgggg
     1261 tcgcccccgc cttggtccac gcctcgttcg agatggtgag ggtcacgacc aggtcctcgt
     1321 agctgaggcg tccgtcggag acacgcgtgc tccagtagcc gaggccgctc ggatcggcgc
     1381 tgcgctcgag cagatcctgg aacttctgcc cgacggcggg ctccaccgcc gtacgcttca
     1441 gcagtgcggc gcggatggtg gccgacgggt tcgtgctgcg gtcgagcgcg gcagcgagcg
     1501 cggtcacgtc ggcgggcgaa gcggccccgg cgtggcgctc gaagatctgg ttcgcccagc
     1561 gggcgttggc gggttgagcg gcgacctgac cgctcggtgg agctgcggcc ggcgcggccg
     1621 tcctctccgg tgccgccgcc accggggtgc ccgcgaccac cgggaccacg gcggtgatcc
     1681 ccagcgcggt gagcaccgcc aacgtcccct gcacccgacc gagccgtcgt gccatcagct
     1741 gtcccccttt cgcagcgcgc cccctaggca cgcaacgcgg tcgaaacact acccgccgtg
     1801 ctccccatgt gagaccgatc cgtctcgcga cgccagatgg gacgcaccag gcccgtggtc
     1861 ccgcacgcgg accgctccga tccgcctacc gtcgggcggg tgagcgacgg caccctcttc
     1921 gacgacgccg tcggccgcga gccccggagc gacacgtgga ccgttcccga gctgtccgtc
     1981 cacgtcggcc gcctcctcgt cggcgccttt cccgacgacg tgtgggtcga agggcagatc
     2041 cgcaacctga accgctccag caacggccac gtctacttcc agctcgccga gccgtgcccc
     2101 gccggccagc agccgaacac ccagctctcg gtcacgctgc tcgcccctga gcgcaccctc
     2161 gtcaacacgc agatcaagcg ggccggcggc ggggtccgca tggaggacgg catcgaggtc
     2221 cgcatccagg ggcgcatccg ctggtacgga ccgcggggca ccctgcagct gcgcatgcac
     2281 ggcatcgacc ccgactacac gctcggccgc ctgcaggccg acaaggaccg cacgctcgcg
     2341 gtgctggccg ccgaggggct gctcgaccgc aacg
//
LOCUS       DATMFS010000170         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_100263, whole genome shotgun sequence.
ACCESSION   DATMFS010000170 DATMFS010000000
VERSION     DATMFS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100263"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2584
                     /locus_tag="VNQ33_03175"
     CDS             <1..2584
                     /locus_tag="VNQ33_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HWJ97134.1"
                     /translation="SPDRSRVALIRDDGWIEVHDLEGARTRLVGSFLLDGAAFVVWGG
                     NDRARFVVVDESGFLNQFVVAPPSRDEVATRSEAEVESERLADTDLLYRRPLQLHLAR
                     QLRRLAHPRPPAPTTVAMHLDGSWGAGKTTLAKFMARDLAHADPAEPGSNRSATGFPD
                     VEWNVVYLNAWRAAQGAPWWWSLTAGIREGLAGQRTSGQRLGFRALDLVVRLVRTGTL
                     LSYSAVAAVAAALTGLAIAFWGWPPSVPDLAKIAAGLAVVGTFATAVNRFVLWDSPTG
                     ARLHERADRSPMDDVCAHLGWLRRWAPRRPERRHRTLAAVAIGAFLVLGACAVAFVLS
                     MPVRDETAALDDPRAVWWITLAVGAAVGVAVFALTAPAHRSAICPEFANAEDPDLAER
                     PAALPPRAGGALVGATLLAAAATAVGWVGLEVLGWGWWRWAIAVAAAIGAAVGGWSDQ
                     RHSPQSGAGIARLGTAIGAGWLAAAPFAVWHLLAGRDLVPERWGWGPAFWAALLGLLA
                     LAGPAAVAVADRRGNQRKAPILVVVDEVDRCTAEEVVQLLEAIQTVARSEVPVPHAPW
                     RQPVSLAFLVLGDGRWIRTAFEHGYDDFNPAEPDLLDPLGARFIQKLFEITARVPDLA
                     PDQFISFVSSVLELPGAAAPRRPRPQDGIDLAPSATGAPETASGGLRAPRAPGPQVGD
                     GQAEQPVRDVFVSPDDQADAAKAAALRESTADVDADLQHAEHQLKAYAPYLEPNPRGI
                     RRTATAFQVNMAVHRTLWVLDPDSDPDHRDLLTRWTILVTRWPALAERLVDPTATSEA
                     DVLDQEVRKAMAHPEVRAVLGDPDAQQRLIDAFSHLRVPAHRRPAAVTIDELRTAAGN
                     RPFR"
BASE COUNT          318 a         1028 c          936 g          358 t
ORIGIN      
        1 gagccccgac cgatcccgcg tcgccctgat ccgagacgac ggctggatcg aggtccacga
       61 cctggagggt gctcggaccc ggctcgtcgg ctcgttcctc ctggacgggg cagccttcgt
      121 cgtctggggc gggaacgacc gtgcccgctt cgtggtcgtc gacgagtccg ggttcctcaa
      181 ccagttcgtc gtcgcaccac ccagcaggga cgaggtcgcg acccgctccg aggcggaggt
      241 ggagagcgag cggctcgctg acaccgacct gctgtaccgg cgtccgctcc agctgcacct
      301 cgcccgtcag ctgcggcggc tcgcccatcc ccgtccaccg gcgccgacga cggtcgccat
      361 gcacctcgac gggtcgtggg gagcggggaa gacgacgctc gccaagttca tggcgcgcga
      421 cctcgcccac gccgaccccg cggaacccgg atcgaaccgg tcggccaccg ggttccccga
      481 cgtcgagtgg aacgtggtgt acctcaacgc gtggcgagcg gcgcagggcg caccctggtg
      541 gtggtcgctc accgcgggga tccgcgaggg gctcgccggc cagcggacgt ccgggcagcg
      601 actcggcttc cgtgccctcg acctggtcgt tcggctggtc cggaccggca ccctcctcag
      661 ctacagcgcg gtggcggcgg tcgcggcggc gctcaccggc ctcgccatcg cgttctgggg
      721 ctggccgccg agcgtgcccg acctggcgaa gatcgcggcg gggctcgccg tcgtcgggac
      781 cttcgccacc gccgtcaacc ggttcgtgct gtgggactcc ccgaccggag cccggctcca
      841 cgagcgcgcc gaccgctcgc cgatggacga cgtctgcgct cacctgggct ggctccgccg
      901 ctgggcgccg cggcgacctg agcggcggca ccgcaccctg gccgccgtgg cgatcggcgc
      961 gttcctcgtg ctcggcgcgt gcgccgtcgc cttcgtcttg tccatgcccg tacgcgacga
     1021 gacggcggcc ctcgatgacc cccgggcggt ctggtggatc accctggcgg tcggcgccgc
     1081 ggtcggcgtg gccgtcttcg cgctcaccgc acccgcccac cggtcagcca tctgcccgga
     1141 gttcgccaac gcggaggacc ccgacctcgc cgagcgccct gccgcccttc caccccgggc
     1201 cggcggggcg ctggtcggcg ccaccctcct cgccgcggcc gcgaccgccg tcgggtgggt
     1261 cgggctcgag gtgctcggct ggggttggtg gcggtgggcc atcgccgtcg ccgccgccat
     1321 cggagccgcg gtcggcgggt ggagcgatca gcgccattca ccgcagtccg gtgccggcat
     1381 cgcccggctc gggaccgcca tcggcgccgg atggctcgcc gccgcgccgt tcgcggtgtg
     1441 gcacctcctc gccggccgcg acctggtgcc cgagcgctgg ggatggggtc cggccttctg
     1501 ggcggccctt ctcggcctgc tcgcgctggc cggccccgcc gcggtcgcgg tcgccgatcg
     1561 acgggggaac cagcggaagg cgccgatcct ggtggtggtc gacgaggtcg atcgctgcac
     1621 cgcagaggag gtcgtccagc tgctcgaggc gatccagacc gtcgcccggt ccgaggtccc
     1681 tgtgccgcac gcgccgtggc ggcagccggt ctcgctcgcc ttcctcgtgc tcggagacgg
     1741 acgctggatc cggaccgcct tcgagcacgg ctacgacgac ttcaacccgg cggagcccga
     1801 tctcctcgat ccgctcggtg cacggttcat ccagaagctg ttcgagatca ccgcccgcgt
     1861 accggacctc gctcccgacc agttcatctc cttcgtgagc tccgtcctcg agctgcccgg
     1921 cgcagccgct ccgcgacggc cccgaccgca ggacggcatc gacctcgccc cgagcgcgac
     1981 gggtgccccg gagaccgcat cgggcgggct ccgcgccccg cgggcaccgg gacctcaggt
     2041 cggcgacggg caggcggagc agccggtgcg ggacgtcttc gtctcgcccg acgaccaggc
     2101 cgatgcggcc aaggcggcag cgctgcgcga gtcgaccgcc gacgtcgacg ccgacctgca
     2161 gcacgcggag caccagctca aggcctacgc gccctacctc gaacccaacc cccgcggcat
     2221 ccggcgcacc gccacggcgt tccaggtcaa catggcggtg caccgcacgc tctgggtcct
     2281 cgaccccgac tccgaccccg accaccgaga cctcctgacg cgctggacga tcctggtgac
     2341 ccgctggccg gcgctcgctg agcggctggt ggaccccacc gcgacgagcg aggccgacgt
     2401 gttggaccag gaggtgcgca aggcgatggc ccaccccgag gtccgagccg tgctcggcga
     2461 ccccgacgcg cagcagcggc tgatcgacgc cttctcccac ctgcgggtgc cagcgcaccg
     2521 acgaccagca gccgtgacga tcgacgagct ccgtaccgcg gcggggaacc ggccgttccg
     2581 ctgagcccgg tgcgggcgcg ggagcggtgt gcgccgcgcc ggcgaacccg tccggaaatc
//
LOCUS       DATMFS010000171         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193466, whole genome shotgun sequence.
ACCESSION   DATMFS010000171 DATMFS010000000
VERSION     DATMFS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193466"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VNQ33_03180"
     CDS             <1..698
                     /locus_tag="VNQ33_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97135.1"
                     /translation="LLAVVAFGIFFFRLRRTDLTAMVAVVVVAAGSGFATAMKLPEGA
                     AVKAANLRWMWTVSAFAWIALVWLVWKVLPVLWKEVLGVPSLVFGSTAVVVAMAAVVS
                     SSDLSTDRDGAIAKDTTTLIANVEREVPEGRYRVKYAGGSVVLSIGPALVHDLEGRGD
                     DLYLDIGPFNRAYGDHRTYSDQEIDGTLLVTAEADAEYPEGTRLVGRQRFRVNSEDER
                     LTTVRVYLVDDAP"
     gene            715..1263
                     /locus_tag="VNQ33_03185"
     CDS             715..1263
                     /locus_tag="VNQ33_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97136.1"
                     /translation="MTDPTPADGDQAKGGAKRASRRRQVVGIWVGAGLGVLVIVAVLL
                     TTLASGGGDGGTTTTAAPPSNANPVPTGIVVTGTVVTPDGDYTEFCKRLTNQASAVTA
                     TPGTPEQLSQLAKTIDFASLEEVAPDGLLPSLRTLASQRDEVVALFDQLDDFSEVTNA
                     DFPAGFLDAVHVMTQATEEKCP"
     gene            complement(1285..1995)
                     /locus_tag="VNQ33_03190"
     CDS             complement(1285..1995)
                     /locus_tag="VNQ33_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97137.1"
                     /translation="MADELDELGAGDEAPLRRERVPTEEEPEADGHRRLRRLLGGACL
                     LLGIAVVVAAVLTTSDDRGTDHADGKSGDTTTTVAGGSDGTKTSVESATSTAADAKPQ
                     WPAATGGRPPAFGKTGDPAPTDPGDLPDGFYVWTDFDGWHGWLVGGGADDRLTLTSDN
                     AITKATPVGGPVDVQLQGNVFTLQRGSATERVVGVDFNPGYFARNMIVAVDGAQPTFV
                     GAKATKVPQIWAMRLEPR"
     gene            2133..>3087
                     /locus_tag="VNQ33_03195"
     CDS             2133..>3087
                     /locus_tag="VNQ33_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWJ97138.1"
                     /translation="MPLDPNRWTLKTQEAVNAAITRAAAEHNPEVTPDHLLVALLGQS
                     EGVVLPILEKVGASPAALRNRAEDGLAKLPKAYGGGEARLSRALNNVIELADAQRAEL
                     HDEYLSTEHLLLALAGQLDLDREELLAALQSVRGSHRVTSQNPEEQYQALERYGRDLT
                     EAARQGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVEGD
                     VPEGLKNKRVISLDLTGMVAGAKYRGEFEERLKAVLKEITDAQGEVITFIDELHTIVG
                     AGQGGGEGGLDIANVFKPALARGELNLIGATTLNEYQKYIEK"
BASE COUNT          463 a         1071 c         1095 g          458 t
ORIGIN      
        1 tgctcctcgc ggtcgtggct ttcggcatct tcttcttccg cctccgacgg accgatctga
       61 cggcgatggt cgcggtcgtc gtcgtcgcgg cggggtcggg cttcgccacg gcgatgaagc
      121 tgccggaggg tgctgcggtg aaggcagcca acctccggtg gatgtggacg gtcagcgcgt
      181 tcgcgtggat cgccctcgtc tggttggtct ggaaggtcct ccccgtgctc tggaaggagg
      241 tcctcggcgt cccgtcgctc gtcttcggtt cgacggccgt ggtggtcgcg atggcggcgg
      301 tggtcagttc gtcggatctg tccaccgacc gcgatggcgc catcgccaag gacacgacca
      361 cgctcatcgc caacgtcgag cgggaggtgc ccgagggccg gtaccgggtg aagtacgccg
      421 gtggatcggt ggtgctgagc atcggtccgg ccctggtcca cgacctcgag ggccgtggcg
      481 acgacctcta tctggacatc ggaccgttca accgcgccta cggcgaccac cgcacctact
      541 ccgaccagga gatcgacggc accctcctcg tcacggccga ggcggacgcc gagtaccccg
      601 aggggacgcg actggtgggc aggcagcggt tccgtgtgaa ctctgaggac gagaggctga
      661 ccaccgtccg ggtgtacctg gtggacgacg cgccctgagc tacgaggaga cccgatgacc
      721 gaccccaccc ccgcagatgg cgatcaggcg aagggtgggg cgaagcgcgc gtctcgccgg
      781 cgtcaggtcg tcggcatctg ggtcggtgcc ggtctcggcg tcctcgtcat cgtcgccgtg
      841 ctgctcacca cgctcgcatc gggcggcggc gacggcggta ccaccaccac ggcggcccca
      901 ccgtcgaacg ccaatccggt ccccaccggg atcgtcgtca cgggcacggt ggtcaccccc
      961 gacggcgact acaccgagtt ctgcaagcgc ctcaccaacc aggccagtgc ggtgaccgcg
     1021 acgccgggca cgcccgagca gctctcgcag ctggccaaga cgatcgactt cgcgtcgctg
     1081 gaggaggtcg ctcccgacgg gctcctgccg agccttcgga cgctggccag ccagcgcgac
     1141 gaggtcgtcg cgctgttcga ccagctcgat gacttctccg aggtgaccaa cgccgacttc
     1201 ccggccgggt tcctcgacgc ggtccacgtg atgacgcagg cgaccgagga gaagtgcccg
     1261 tgacgggctg aggtcagggc cgggtcaccg cggctcgagc cgcatggccc agatctgcgg
     1321 caccttcgtc gccttggccc cgacgaaggt gggctgcgct ccgtcgaccg ccacgatcat
     1381 gttgcgggcg aagtagccgg ggttgaagtc gacgccgacc acccgctcgg ttgcgctccc
     1441 ccgctgcagg gtgaagacgt tgccctggag ctgcacgtcg acaggaccgc cgacgggggt
     1501 cgccttggtg atcgcgttgt cgctggtgag cgtgagccgg tcgtcggcgc cgccgccgac
     1561 gagccagccg tgccacccgt cgaagtcggt ccagacgtag aagccgtcgg ggaggtcgcc
     1621 ggggtcggtg ggggccgggt cgccggtctt gccgaacgcc gggggccggc caccggtggc
     1681 ggcgggccac tgcggcttcg cgtcggccgc ggtgctggtg gccgactcca ccgaggtctt
     1741 ggtcccgtcg ctgcctccgg ccacggtggt ggtcgtgtcc ccggacttgc cgtcggcgtg
     1801 gtcggtgccg cggtcgtcgg aggtcgtgag cacggcggcg accaccacgg cgatccccaa
     1861 caggaggcac gcgccgccca ggagccggcg gagcctgcgg tgcccgtcgg cttcgggctc
     1921 ctcctcggtg gggacccgtt cgcgtcggag gggtgcctcg tcgcccgcac ccagttcgtc
     1981 gagctcgtcg gccacggcag cgttcctccc gttcagcggc cggtggagca tagttcggag
     2041 gcggtcgtga cctgcatctc gatcggcccg ctgggcgtca agacttgagt ttgatgtagg
     2101 caagttttgt gatggcgaga tagcatgggt ccatgccctt ggaccccaac cgctggaccc
     2161 tgaagacaca ggaggccgtc aacgccgcga tcacgcgcgc cgcggccgag cacaaccccg
     2221 aggtcacccc cgaccacctg ctcgtcgcgc tgctcggaca gagcgagggg gtcgtcctgc
     2281 cgatcctcga gaaggtgggc gcgtcgcccg ccgccctgcg caaccgcgcc gaggacggcc
     2341 tggccaagct ccccaaggcc tacggcgggg gcgaggcccg gctgtctcgc gccctcaaca
     2401 acgtcatcga gctggccgac gcccagcggg ccgagctcca cgacgagtac ctctccaccg
     2461 agcacctcct cctggcgttg gccggccagc tggacctcga ccgcgaggag ctgctcgccg
     2521 cgctccagag cgtgcggggc agccaccgcg tcacgagcca gaaccccgag gagcagtacc
     2581 aggcgctgga gcgctacgga cgggatctca ccgaggcggc gcgccagggc aagctcgacc
     2641 cggtcatcgg gcgcgacgag gagatccgcc gcaccatcca ggtgctgagc cgccgcacca
     2701 agaacaaccc ggtgctcatc ggtgagcccg gcgtcggcaa gaccgccatc gtcgagggcc
     2761 tggcgcgccg catcgtcgag ggcgacgtgc ccgagggcct gaagaacaag cgggtcatca
     2821 gcctcgacct caccggcatg gtcgccggcg ccaagtaccg cggcgagttc gaggagcggc
     2881 tgaaggccgt cctcaaggag atcaccgatg cccagggcga ggtcatcacc ttcatcgacg
     2941 agctccacac catcgtcgga gccgggcagg gcggtggcga gggaggtctc gacatcgcca
     3001 atgtgttcaa gcccgccctg gcgagggggg agctgaacct gatcggggcg accaccctca
     3061 acgagtacca gaagtacatc gaaaagg
//
LOCUS       DATMFS010000172         2152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_309359, whole genome shotgun sequence.
ACCESSION   DATMFS010000172 DATMFS010000000
VERSION     DATMFS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309359"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /gene="rpsF"
                     /locus_tag="VNQ33_03200"
     CDS             complement(<1..323)
                     /gene="rpsF"
                     /locus_tag="VNQ33_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWJ97139.1"
                     /translation="MLAMRAYEMMVICDGDLDESAVQGVINQATAQIESAGGTIAKTD
                     KWGKRRFAYEINHKNEGFYVVFEIEAEPGALDDVDRNLRLADEVVRHKIIRLPDSEAH
                     RRGLLG"
     gene            complement(479..1012)
                     /locus_tag="VNQ33_03205"
     CDS             complement(479..1012)
                     /locus_tag="VNQ33_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWJ97140.1"
                     /translation="MIPVEAPGIEAGTTAPLGSAGLGEGVAGEVAGRTPLRGFGEVQV
                     SLTTDGGRICELCLLSAMTADQRERGLMEVTDEDLGGYDGMLFEYPKEVDGQFWMRDT
                     PMPLSIAFFDAQGKLVSTTDMQPCGDTSSCPAYPAAGTFKYAIEVPQGLLEEVGATPG
                     ATLHIHTRRCPLADGGG"
     gene            complement(1234..2061)
                     /gene="panB"
                     /locus_tag="VNQ33_03210"
     CDS             complement(1234..2061)
                     /gene="panB"
                     /locus_tag="VNQ33_03210"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWJ97141.1"
                     /translation="MRNRKVRNGDEPLVMVTAYDAPGARVADEAGVDMILVGDSVAMV
                     VLGYDDTLQVTVDDLAHHTAAVARAKPRPLIVADLPWMSYHVSTTDTVRNAAQLIRAG
                     GECVKLEGGRKRLPMIEALIDAEIPVMGHLGLTPQSVHAMGGFKVQGKEADAAMGLVS
                     DAKALAHAGCFAIVLEGVPTEVASLVTAAVDVPIIGIGAGDDCDGQVLVYHDVLGIED
                     RIAPKFVRRYADLKTAAVEGVAAYAADVRTRAFPSAAESYHLSAEAAETLGLYGRPA"
BASE COUNT          309 a          735 c          789 g          319 t
ORIGIN      
        1 cccaggagac cgcggcggtg ggcctcggag tccgggagcc ggatgatctt gtggcggacg
       61 acctcgtccg cgaggcgcag gttgcggtcg acgtcgtcga gcgctcccgg ctcggcctcg
      121 atctcgaaca ccacgtagaa cccttcgttc ttgtggttga tctcgtaggc gaagcggcgc
      181 ttgccccact tgtcggtctt ggcgatggtg ccgccagcag actcgatctg ggccgtggcc
      241 tggttgatca ctccctgtac ggcggactca tcgaggtcgc cgtcgcagat aaccatcatt
      301 tcgtaggctc gcatcgcgag catcacctcc tggacccggg tctcccgggg cccctgtgcc
      361 ggaattggcg atggggcaag ggcttcaatt ggaccgtgcg aacctagcgg aacacggccg
      421 tccgagcctc atcggagacc atcgtccccc gggggtctga cagcgaaggc cgggcgcgtc
      481 agccgccgcc gtccgccagg ggacagcgcc gggtgtggat gtggagcgtc gcgcccggcg
      541 tggccccgac ctcctcgagc aggccctgcg gcacctcgat ggcgtacttg aacgtgcccg
      601 cggcggggta ggcgggacag ctggaggtgt cgccgcacgg ctgcatgtcg gtggtggaga
      661 cgagcttccc ctgggcatcg aagaaggcga tcgacagcgg catcggggtg tcccgcatcc
      721 agaactgccc gtcgacctcc ttcgggtact cgaagagcat cccgtcgtag ccgcccaggt
      781 cctcgtcggt gacctccatc agaccccgct cccgctgatc ggcggtcatg gccgacagga
      841 ggcagagctc gcagatcctg ccgccgtcgg tggtgaggct gacctgcacc tcgccgaagc
      901 cccgcagcgg ggtccggccc gccacctcgc cggccacgcc ttcgcccagc cccgcggaac
      961 cgagcggcgc ggtggtcccg gcctcgatcc cgggggcctc gaccgggatc acggccgtgg
     1021 tggtgacggc cgcgtcggag ggatcgccgg agttggagcg gacgagcgag acgacgacca
     1081 gcgccaggcc gatcagcagg cagcccgcgc cgatgacgag acccagggat ggacccgcgg
     1141 gcgcagcgtc aggccgctca gtggtggacg ggggcgcgtc gatcccccca cggtacccgg
     1201 ggcctccgga ccccggccgg tggccgggcc cgctcaggcc gggcgtccgt agaggccgag
     1261 ggtctcggcc gcttcggcgg agaggtggta cgactccgcc gccgacggga acgcacgagt
     1321 gcggacgtcg gcggcgtagg cagccacgcc ttccaccgca gccgtcttga gatcggcgta
     1381 gcggcgcacg aacttggggg cgatgcggtc ctcgatgccg aggacgtcgt ggtacacgag
     1441 cacctggccg tcgcagtcgt cgccggcgcc gatgccgatg atcggcacgt cgaccgccgc
     1501 ggtgacgagc gaggcgacct cggtgggcac gccctcgagc acgatggcga agcagccggc
     1561 gtgggccagc gccttggcgt cggacacgag ccccatcgcc gcgtcggcct ccttgccctg
     1621 gaccttgaag ccgcccatgg catggaccga ctgcggggtc aggccgaggt ggcccatcac
     1681 ggggatctcg gcgtcgatca gcgcctcgat catcgggagg cgcttgcggc cgccctcgag
     1741 cttcacgcac tcgccgccgg cccggatgag ctgggcagcg ttgcggacgg tgtcggtggt
     1801 ggacacgtgg tagctcatcc acggcaggtc ggccacgatc agcggacggg gcttggcccg
     1861 ggcgacggcg gccgtgtggt gcgcgaggtc gtcgaccgtg acctggaggg tgtcgtcgta
     1921 gccgagcacc accatggcga cggagtcgcc gaccaggatc atgtcgaccc cagcctcgtc
     1981 ggcgacgcgg gcaccgggtg cgtcgtacgc cgtgaccatc acgagcggct cgtcgccgtt
     2041 gcggaccttg cggttccgca cggcaggcac cgtgatgggc gggcgcgatg cgacaggaga
     2101 agatgacgtc gagctcatgg gagcctccct tgctgatccg tccagtgccc tt
//
LOCUS       DATMFS010000173         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193510, whole genome shotgun sequence.
ACCESSION   DATMFS010000173 DATMFS010000000
VERSION     DATMFS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193510"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VNQ33_03215"
     CDS             <1..1160
                     /locus_tag="VNQ33_03215"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWJ97142.1"
                     /translation="LLASVSARLSGCVRPGDTVARLGGDEFGILLEEMEGPEHAAHVA
                     RRILESLDVAFPLDGRQVFTRASVGVAVADSTSGHAGADLLSDADVALYAAKGSGKST
                     FREFERTMRVTAIERLELGQDLRQALHQNQFFCHYQPIVDLVTGRVVALEALIRWDHP
                     HRGLVGPNSFIELAEESGMISEIGMAVLQMAAWQAANWRAEGRVPAALELHVNLSGRQ
                     LEDPYLVEKVKRVLASTGFPGNLLVLEITESVAVETGAAHIELLEQLKALGIRFAIDD
                     FGTGYSSLNYLRALPVDVLKIDRAFAQTVDGETDTVLLEAIVRLGHSLGIDMIAEGIE
                     HEEQAVTLRRLGCRRAQGYLFHRPLSVSEIPLVIGSTRPVPDPAPHGPAPT"
     gene            1173..2033
                     /locus_tag="VNQ33_03220"
     CDS             1173..2033
                     /locus_tag="VNQ33_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ97143.1"
                     /translation="MGDDWEPSWVDVDGMRLRYLDTDPTGGPPADGDALPLLVIPGHT
                     ARIEGFGPMIAAGLAAAHRVLVLDCPGSGESEKPDRPYDLRFYEDTLVAFLDHLGIDQ
                     AIPVGGSLGGNLVLRLGHRFPERFPRLVLWAPGSAWKAKPVLAWLTRHVIGSRLLRKA
                     VFWPVVKTQSRFWYSPDYPDRQRELDETFAYYRRVMSPGFLAMYWGMAADQLGRSLFD
                     LAPDIAQPTRMMWGDQDNGAQMGRGVARLHQLLPDNVLHVFPGRRHSLEAECPEDLAE
                     QILIGAAGSG"
     gene            2050..>2462
                     /gene="yihA"
                     /locus_tag="VNQ33_03225"
     CDS             2050..>2462
                     /gene="yihA"
                     /locus_tag="VNQ33_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWJ97144.1"
                     /translation="MAATPPLPLRFVMSGTRVEDLPPTRGEVAIVGRSNVGKSSLINS
                     IANRKGLAKVSKTPGRTQLLNLFELPDGTSVLDLPGYGFANAPASVRATWPEMIEGYL
                     LGRRNLHAVVALIDAEVGPTRNDVQTLAWLRSHGLP"
BASE COUNT          342 a          927 c          827 g          366 t
ORIGIN      
        1 ggctcctcgc atcggtgtcc gcccgcctga gcggctgcgt ccgccctggc gacaccgtgg
       61 cccgcctcgg cggcgacgag ttcggcatcc tcctggagga gatggagggt cccgagcacg
      121 ccgcccacgt ggcccgtcgc atcctcgagt cgctcgacgt ggccttcccg ctcgacggcc
      181 gccaggtgtt cacccgggcc agcgtcggcg tcgccgtggc cgacagcacc tcgggccatg
      241 ccggcgccga cctcctctcc gacgccgacg tagcgctcta cgccgcgaag ggctcgggca
      301 agtccacgtt ccgggagttc gagcggacga tgcgcgtcac cgcgatcgag cggctcgagc
      361 tcggccagga cctgcgccag gcgctgcacc agaaccagtt cttctgccac taccagccga
      421 tcgtcgacct cgtcaccggc cgggtcgtcg ccctcgaggc gctgatccgc tgggaccatc
      481 cgcaccgcgg cctcgtcggc cccaactcgt tcatcgagct ggccgaggag agcgggatga
      541 tcagcgagat cggcatggcc gtgctccaga tggcggcatg gcaggccgcc aactggcggg
      601 ccgagggccg tgtccctgcc gcgctggagc tccacgtcaa cctgtcgggc cgccagctcg
      661 aagatccgta cctcgtcgag aaggtcaagc gggtcttggc ctccaccggc ttcccgggca
      721 acctcctggt gctggagatc accgagagcg tggccgtcga gaccggtgcc gcgcacatcg
      781 agctcctcga gcagctcaag gcgctcggca tccgcttcgc catcgacgac ttcggcaccg
      841 ggtactcgtc gctcaactac cttcgggccc tccccgtcga cgtgctcaag atcgaccggg
      901 cgttcgcgca gaccgtcgac ggcgagaccg acacggtgct gctcgaggcg atcgtgcgcc
      961 tcggccactc gctcggcatc gacatgatcg ccgagggcat cgagcacgag gagcaggccg
     1021 tcacccttcg gcgcctcggc tgccggcgtg cccagggcta cctgttccac cgcccgctgt
     1081 cggtcagcga gatcccgctc gtcatcggtt cgacccggcc ggtgcccgat ccggcgccgc
     1141 acggcccggc gccgacctga caacctgtcc ccgtggggga tgactgggaa ccgagctggg
     1201 tcgacgtcga cggcatgcgc ctgcgctacc tcgatacgga tccgacgggt ggcccgccgg
     1261 ccgacgggga cgccctgccg ctgctggtga tcccgggtca cacggcgcgc atcgagggct
     1321 tcgggccgat gatcgccgcc ggcctggccg cggcccaccg ggtgctggtg ctcgactgcc
     1381 cgggcagcgg cgagtccgag aagcccgacc gcccctacga cctgcggttc tacgaggaca
     1441 ccctggtggc cttcctcgac cacctcggca tcgaccaggc gatccccgtc ggcggaagcc
     1501 tcggcggcaa cctcgtcctc cgcctgggcc accggttccc cgagcggttc ccgcgcctcg
     1561 tcctctgggc accgggctcg gcgtggaagg cgaagccggt gctcgcctgg ctgacccgcc
     1621 acgtgatcgg gtcacggctg ctgcgcaagg cggtcttctg gccggtggtc aagacccaga
     1681 gccgcttctg gtactcgccg gactacccgg atcgccagcg cgagctcgac gagacgttcg
     1741 cctactaccg ccgagtcatg tcgcccgggt tcctcgccat gtactggggg atggcggccg
     1801 accagctggg gcgcagcctg ttcgacctgg cgcccgacat cgcccagccc acccggatga
     1861 tgtggggcga ccaggacaac ggcgcgcaga tgggcagggg cgtcgcccgc ctccaccagc
     1921 tgctgcccga caacgtgctg cacgtcttcc cgggacggcg ccactcgctc gaggccgaat
     1981 gccccgagga cctggccgag cagatcctga tcggagctgc gggttcgggg tgagtggtag
     2041 caacggggga tggccgccac ccccccgctc ccgctccgct tcgtgatgtc gggcacgcgg
     2101 gtcgaggacc tcccgccgac gcggggcgag gtggcgatcg tcggccggtc caacgtcggc
     2161 aagtcgtcgc tcatcaactc gatcgccaac cgcaagggcc tggccaaggt gtccaagacg
     2221 cccggccgca cccagctgct caacctgttc gagctgcctg acggcacctc ggtcctggac
     2281 ctgcccggct acggcttcgc caacgccccc gcctcggtgc gggcgacgtg gcccgagatg
     2341 atcgaggggt acctgctcgg gcgcaggaac ctccatgccg tcgtcgccct catcgacgcc
     2401 gaggtcggac ccaccaggaa cgacgtgcag acgctcgcgt ggctccgctc gcacgggctg
     2461 cc
//
LOCUS       DATMFS010000174         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_216608, whole genome shotgun sequence.
ACCESSION   DATMFS010000174 DATMFS010000000
VERSION     DATMFS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216608"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VNQ33_03230"
     CDS             complement(<1..722)
                     /locus_tag="VNQ33_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWJ97145.1"
                     /translation="MLVRRIARPLLSTIFITSGIESLRRPSGPAQVAEPVIDLVDRQA
                     VQPAAQKVATGAAGLADAAVAVTADIAEATPDERARDAAGAAASAVDGAAEPVRRVAI
                     GGIDLADETYVRINGAVQVGAGLLLAAGKAPRLASTALAASLVPTTLAGHRFWEFEGD
                     ERKAQQIHFLKNAGLLGGLILAAADTEGRPGLAWRAKNRRDDSGVIATAAAATASVAG
                     KAAKADAKAAKRLAAANAKLARA"
     gene            944..1849
                     /locus_tag="VNQ33_03235"
     CDS             944..1849
                     /locus_tag="VNQ33_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWJ97146.1"
                     /translation="MTDPRFESVAAVREGLRKVDYLSDEGIAGIVYLADRLQKPILVE
                     GPAGTGKTQLAKSVAEMIGARLIRLQCYEGLDEAKALYEWNYKKQLLRIQASGGEGDS
                     WDTVEDDLFSDEFLMERPLLEAIRADEPVVLLIDEVDRIEVETEALLLEILSDYQVSI
                     PELGTVTAKQIPLVFLTSNNTRELSEALKRRCLFLHVDYPDLEREKEIVLTKVPDITD
                     NLADQVARIVRSLRQLELKKSPSVSETLDWANTLMLLGVEQIDAETASGTANILLKYQ
                     SDIAKAVREFAADKQGARQFAPGPK"
     gene            1855..3354
                     /locus_tag="VNQ33_03240"
     CDS             1855..3354
                     /locus_tag="VNQ33_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ97147.1"
                     /translation="MADTAEPEVHAAGEPLLDLLNGFIVELREAGLPVSLTENLDAMA
                     AIEHIPLEERETFKYALAATLVKNQAHWRSFETVFEVYFSLRGAKYGIDDDSLESDLA
                     DLLDDAENDGQGEGQGGQAGQGGGDALTPEELQQLLYQAMMKGDEALMRAIARQAVKR
                     FAGMEPGRPVGGTYYLYRTLRNLDLDNLLEKMMEQRRQAADDAGEPMSPLEERLEKDE
                     YEHRVEALKKEIEAEIRRRLVADRGVEAMAKTLRKPLPEDVDFMHASREEMIGLRKAL
                     VPLTRRLAVRLARKRRHNRKGPLDFRRTVRASLSYGGVPAEPKFRHPKPHKPEIFVIA
                     DISGSVAAFARFTLMLVYAISGQFSKVRSFVFIDGIDEVTSFFEGVEDVTEAVHRVNT
                     EADVVWVDGHSDYGHAFEVFHNKWGKEIGPKTTVIILGDARNNYHASQSWVIKEMQHK
                     ARHVYWLNPEPKSYWDTGDSIVGDYGAHCDDVFECRNLRQLERFVEHLT"
BASE COUNT          654 a         1558 c         1502 g          592 t
ORIGIN      
        1 gccctggcca gcttggcgtt ggccgccgcc aggcgcttgg ccgccttggc atcggccttg
       61 gccgccttgc cggcgaccga tgcggtcgca gcagcggcgg tggcgatcac gccgctgtcg
      121 tcgcgccggt tcttggcgcg ccacgccagt ccgggccggc cctcggtgtc ggctgccgcg
      181 aggatgaggc cgccgagcag gcccgcgttc ttcaggaagt ggatctgctg ggccttgcgc
      241 tcgtcgccct cgaactccca gaaccggtgg ccggcgaggg tggtgggcac gagcgacgcg
      301 gccagggccg tggacgccag gcggggcgcc ttgccggcgg cgagcagcag tccagcgccc
      361 acctgcacgg cgccgttgat gcggacgtag gtctcgtcgg ccaggtcgat gccgccgatc
      421 gccacccggc gcaccggctc ggccgcgccg tccacggcgg atgccgcggc cccggcggcg
      481 tcccgagcgc gctcgtcggg cgtggcctcg gcgatgtcgg ccgtcacggc gacggcggcg
      541 tcagcgaggc ccgccgctcc cgtggcgacc ttctgggccg cgggctggac ggcttggcga
      601 tcgaccagat cgatgaccgg ttcggccacc tgcgcgggac cggatggacg ccggagcgac
      661 tcgatgccgg aggtgatgaa gatcgtggac aggagggggc gggcgatgcg tcgaaccaac
      721 atgcgccccc acttcccctg gcggcgccgt cctacacccg gtccgctggt gcgcagagcg
      781 ggcgacgagg tgccctcctg ctcggcctca tcggcctcgg gctgggcctg ctcggcgcca
      841 gcgcctcggt ggcccgcggc ccgatccgca cggcctgatc ctggtcccgc cggccggtca
      901 tttcgattga ccggccggtc aagaccggcg gtaacctgcg ccaatgaccg atccccgctt
      961 cgagtccgtc gccgccgtcc gagaggggct gcgcaaggtg gactacctgt ccgacgaagg
     1021 catcgccggc atcgtctacc tcgccgaccg gctccagaag ccgatcctgg tggagggccc
     1081 ggccggcacc ggcaagaccc agctggccaa gtcggtggcc gagatgatcg gcgcccgcct
     1141 catccgcctc cagtgctacg agggcctcga cgaagccaag gcgctctacg agtggaacta
     1201 caagaagcag ctcctgcgca tccaggcgtc gggcggcgag ggcgactcgt gggacacggt
     1261 cgaagacgac ctgttctccg acgagttcct catggagcgc ccgctgctgg aggccatccg
     1321 cgccgacgag ccggtcgtgc tgctcatcga cgaggtcgac cgcatcgagg tcgagaccga
     1381 ggccctgctc ctggagatcc tgtcggacta ccaggtgtcc atcccggagc tgggcaccgt
     1441 cacggccaag cagatcccgc tggtgttcct cacctcgaac aacacccgtg agctgtccga
     1501 ggccctcaag cgccgctgcc tgttcctcca cgtcgactac cccgacctgg agcgcgagaa
     1561 ggagatcgtc ctcaccaagg tccccgacat caccgacaac ctggccgacc aggtggcccg
     1621 catcgtgcgg tcgctgcgcc agctggagct gaagaagagc ccgtcggtgt ccgagaccct
     1681 ggattgggcc aacacgctca tgctgctcgg cgtcgagcag atcgacgccg agaccgcgtc
     1741 gggcaccgcc aacatcttgc tgaagtacca gtccgacatc gccaaggcgg tccgtgagtt
     1801 cgcggccgac aagcagggcg cccggcagtt cgccccgggg ccgaagtagg cgtcgtggcc
     1861 gacaccgccg agcccgaggt ccacgcggca ggggagcccc tgctcgatct gttgaacggg
     1921 ttcatcgtcg aactgcggga ggccggcctg ccggtcagcc ttaccgagaa cctcgacgcc
     1981 atggcggcca tcgagcacat cccgctcgag gagcgggaga cgttcaagta cgccctggcc
     2041 gccaccctgg tgaagaacca ggcccactgg cgctcgttcg agacggtgtt cgaggtgtac
     2101 ttctcgctgc gcggcgccaa gtacgggatc gacgacgact cgctggagtc ggacctggcc
     2161 gacctgctcg acgacgccga gaacgacggc cagggcgaag gccagggcgg gcaggcgggc
     2221 cagggcggtg gcgacgccct cacgcccgaa gagctccagc agctgctcta ccaggcgatg
     2281 atgaagggcg acgaggccct catgcgcgcc atcgcccgcc aggccgtcaa gcgcttcgcc
     2341 gggatggagc ccgggcgccc ggtcggcggc acgtactacc tgtaccgcac cctgcggaac
     2401 ctcgacctcg acaacctgct cgagaagatg atggagcagc ggcggcaggc ggccgacgac
     2461 gccggcgagc cgatgagccc gctcgaggag cgcctggaga aggacgagta cgagcaccgg
     2521 gtcgaggcgc tgaagaagga gatcgaagcg gagatccgcc gtcgcctggt ggcggaccgc
     2581 ggcgtggagg ccatggccaa gaccctccgc aagcccctgc ctgaagacgt cgacttcatg
     2641 cacgcgtcgc gagaggagat gatcgggctc cgcaaggcgc tggtaccgct cacccgccgc
     2701 ctcgccgtgc gcctggcccg caagcgccgc cacaaccgca aggggccgct ggacttccgg
     2761 cgcaccgtgc gcgcctcgct cagctacggc ggcgtccccg ctgagcccaa gttccgccac
     2821 ccgaagccgc acaagcccga gatcttcgtg atcgccgaca tctccggctc ggtcgccgcg
     2881 ttcgcccggt tcaccctgat gctcgtctac gcgatcagcg gccagttctc gaaggtccgc
     2941 tcgttcgtgt tcatcgacgg catcgacgag gtcacctcgt tcttcgaggg cgtcgaggac
     3001 gtcaccgagg ccgtccaccg ggtcaacacc gaggccgacg tggtgtgggt cgacggccac
     3061 tccgactacg gccacgcctt cgaggtgttc cacaacaagt ggggcaagga gatcgggccg
     3121 aagaccacgg tgatcatcct gggcgacgcc cggaacaact accacgcctc ccagagctgg
     3181 gtcatcaagg agatgcagca caaggcccgc cacgtctact ggctcaaccc cgagcccaag
     3241 tcctactggg acaccggcga ctccatcgtc ggcgactacg gcgcccactg cgacgacgtc
     3301 ttcgagtgcc gcaacctccg ccagctcgag cgcttcgtcg agcacctcac ctgatcgacc
     3361 cgcgggccga aggccgtcgg aaggccgacc gcggatcacg ccccgggaca tccgctcgga
     3421 gggtcggggc gggcgtgccg cggctcagct gggccaggcg gatcccaccg gaccggtcac
     3481 ctccccgcgg tgctcgccgg gctggcgctc accacgacgg cacggagacc ctgctctcga
     3541 tcgtgtgcgc cctcggacgc tcgttaggat cggcgctcgg tccagggagc gagcgggagc
     3601 ggggatgacg gacagcgtgg gagacggtcc gcgggcatgc ccgcagtgcg gcgagacggt
     3661 gccgtccggt cggtcggtgt gcccggcctg cggccggttg ctcggaccgg cggtgccgcc
     3721 gcccaccgcc ccaccgcgcc cggcgggctg gggaccgggt ggctcctcgc cggggttctc
     3781 gcctccgcac ccgccggccg gccccgcctc ggtgccccca gcgaacacga accctgctct
     3841 tggtcacggc cccggcttcg ggggatcgac cgccccgggg gcgtgggcgg cgccgggcat
     3901 ggccccgctg ccgcgctccg tgccgccacg caagcagcgc gcatggatcg ggctgctcgt
     3961 ggtgctcgtc gtgctcggtg ccgtcggcgt gggtgtcgcc gcggtggtgc agcggtcaga
     4021 cgacgatgcc gacgcagcgg gcaaggggtc cgacaagacg accacgaccc tcgctccgac
     4081 gaccaccacg atcccgcgcg tgacgcaggc cggcctgctc gacgactact gcaacgctcc
     4141 cggcctcatg tgtggcccga ggtgccgccg cacgtgccgg gcgagccgga tcggacgtac
     4201 acgaaggtca tggacacggg tgtggacacc gccagcaacg agaccgccgg cagctactac
     4261 cccgtggatc cttcgtcgct cggcgggtcg acggtgatct ccccgc
//
LOCUS       DATMFS010000175         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_332869, whole genome shotgun sequence.
ACCESSION   DATMFS010000175 DATMFS010000000
VERSION     DATMFS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332869"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VNQ33_03245"
     CDS             complement(<1..903)
                     /locus_tag="VNQ33_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97148.1"
                     /translation="MPQAAPAPPPAVAAPAKPKGAIGIWIGVLLIVVGIVLGIVLVVA
                     GVKSFANGFNDLQHVPINGAGIVRIEGTGSQSIYAERPTSTGGTTFNTSTGTTFSGYG
                     PNVAVRVIGPDGRDVAVRVPSGREDYRADYREGVRIGKFDAPVEGEYRIVSRLQDPNG
                     NWDEIAVGHAIELSGIGTIAGGIFGGGLIVLIGIIVVIIFAVRRSRSKKQISQGSAPY
                     PGAYGAPMAGWPAGPGYAPPAAPGYGSPAGPAAGAWSSNPGWVPPPVAPPGSESPGPT
                     WSPPPPPAPQAPAPGSTWQPPTAPP"
     gene            complement(958..2064)
                     /locus_tag="VNQ33_03250"
     CDS             complement(958..2064)
                     /locus_tag="VNQ33_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWJ97149.1"
                     /translation="MAGFTPPPYPYDRLAPLHEAASRLPGGVVDLSIGTPCDPPPSFV
                     AEALADTAAARSYPPSVGTPAYRSAASDWVQRRFGAEVDPGAVAACVGTKEVVAGVPH
                     WLRLRTPERDTVLYPEISYPTYAMGAALAGCRAIPVPVDDAWRIRLDAVDPADADRAV
                     CLWVNTPGNPAGGLDDLGAAAAWGRDRGVPVFSDECYAEFTWDGPARSILAEGTDGVV
                     AVHSLSKRSNLAGARVGFYAGDADLVRFISEVRKHAGFMVPGPMQRAGALAWADDDHV
                     AVQRGRYRDRLEALAAGLRSFGLEVGLPAGAFYLWVAAPHGDAWALADELARRGGLLV
                     SPGEFYGPAGSGHVRIAAVQPLDRIELALQRLAG"
     gene            complement(2068..3090)
                     /gene="dapD"
                     /locus_tag="VNQ33_03255"
     CDS             complement(2068..3090)
                     /gene="dapD"
                     /locus_tag="VNQ33_03255"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:TIGR03535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWJ97150.1"
                     /translation="MTDPAAPTSAAWAVGIATYSADGKVLDTWFPDPILGLTEGALRL
                     AEEHGHTGGSGTYPLSRGQALDYLRVDVQHLTESDDLRRVKRAAVVTFISDLDDAPVD
                     THDCYLRLHLLSHRLVPPHGCNLDGIFGLLTNVVWTNLGPCAVEGFEQTRMRLRVAGH
                     TVTVFGIDKFPRMVDYVVPSGVRIADADRVRLGAHLAEGTTVMHEGFCNFNAGTLGES
                     MVEGRISAGVVVGVGSDIGGGSSIQGTLSGGGKTVISIGEGCLLGANAGLGISLGDRC
                     TVQAGCYILGGTLVTTPDGQVVKAATLSGQSDLLFRQNSKTGAMEVLSQTVDWGSLNT
                     ALHQND"
BASE COUNT          485 a         1180 c         1221 g          410 t
ORIGIN      
        1 cggaggagcc gtcgggggct gccaggtcga ccccggtgcc ggggcctgcg gtgcgggagg
       61 cggaggcggt gaccacgtgg gcccggggct ctccgaaccg ggcggcgcca ccggcggcgg
      121 cacccagccg gggttcgacg accaggcccc ggcggcggga ccggccggcg agccgtagcc
      181 cggcgcggcg ggcggcgcgt agcccggccc cgcgggccac cccgccatcg gggcgccgta
      241 ggcacccggg tagggcgccg agccctggct gatctgcttc ttcgagcggg accggcgaac
      301 ggcgaagatg atgaccacga tgatgccgat cagcacgatc aggccgccgc cgaagatccc
      361 gccggcgatc gtaccgatgc ccgacagctc gatggcgtgg ccgacggcga tctcgtccca
      421 gttgccgttg gggtcctgca agcgtgacac gatccggtac tcgccctcga ccggcgcgtc
      481 gaacttcccg atccgcaccc cttcgcggta gtcagcccgg tagtcctccc tgcccgacgg
      541 gacccgcacg gcgacgtcgc ggccatcggg cccgatcacc cgcacggcaa cgttcggtcc
      601 gtacccgctg aaggtggtgc ccgtcgaggt gttgaaggtg gttcccccgg tcgaggtggg
      661 ccgctcggca tagatcgact gggacccggt gccttcgatg cggacgatgc ccgcgccgtt
      721 gatgggcacg tgctgcaggt cgttgaaccc gttggcgaac gacttcaccc cggcgaccac
      781 caagacgatg cccaagacga tgccgacgac gatcaacaag acgccgatcc agatgccgat
      841 ggcacccttc ggcttcgcag gtgcggcgac ggcgggcggg ggcgcgggtg cagcttgggg
      901 catcacccca ccagtctcgc ccatgaccga cgggttcgga gaggaatggg acccgactca
      961 gccagcgagg cgctgcaggg cgagctcgat ccggtccagg ggctggaccg cggcgatgcg
     1021 gacgtgcccc gagccagccg ggccgtagaa ctcgccgggg ctcacgagca ggccgccccg
     1081 ccgagcgagc tcgtccgcca acgcccacgc atcgccgtgc ggcgcggcca cccacaggta
     1141 gaacgcaccg gccggaaggc cgacctccag gccgaacgag cgcagcccgg cggccagggc
     1201 ctcgagccgg tcccggtacc ggccgcgctg cacggcgacg tggtcgtcgt cggcccacgc
     1261 cagggcgccg gctcgctgca tcgggccggg gaccatgaac ccggcgtgct tgcgcacctc
     1321 cgagatgaag cgcaccagat ccgcatcgcc ggcgtagaag ccgacccggg ccccggcgag
     1381 gttcgaccgc ttcgacagcg agtgcacggc caccacgccg tcggtgccct ccgccaggat
     1441 cgaccgggcc ggaccgtccc aggtgaactc ggcgtagcac tcgtcggaga agacgggcac
     1501 gccccggtcg cggccccagg ccgccgccgc gcccaggtcg tcgaggccgc cggccgggtt
     1561 gcccggcgtg ttcacccaga ggcacaccgc ccggtccgcg tccgccggat cgacggcatc
     1621 gagccggatg cgccacgcgt cgtcgaccgg aacggggatc gcccggcaac cggcgagcgc
     1681 agcgcccatc gcataggtcg ggtagctgat ctccgggtag aggacggtgt cgcgctcggg
     1741 ggtgcggagg cgcagccagt gcgggacgcc agccacgacc tccttggtcc cgacgcaggc
     1801 cgccacggcg ccaggatcga cctcggcccc gaagcggcgc tgaacccagt cagaagcggc
     1861 cgaacggtag gccggcgtgc cgaccgaggg cgggtaggac cgggcggcgg ccgtgtcggc
     1921 gagcgcctcg gccacgaacg agggcggtgg gtcgcacggc gtgccgatgg acaggtcgac
     1981 cacgcctccg ggcaagcggg atgcggcctc gtggagcggg gcgaggcggt cgtaggggta
     2041 cggcggcggc gtgaacccgg ccatcggtca gtcgttctgg tggagcgccg tgttcagcga
     2101 tccccagtcg accgtctgcg acagcacctc catcgccccg gtcttcgagt tctggcggaa
     2161 gagcaggtcc gactggcccg acagcgtggc ggccttcacg acctggccgt cgggggtcgt
     2221 gaccagcgtg cccccgagga tgtagcagcc ggcctggacg gtgcagcggt cgccgagcga
     2281 gatcccgagc ccggcgttgg caccgagcag gcagccctcc ccgatggaga tgaccgtctt
     2341 gcccccgccc gagagcgtcc cctggatcga cgagccaccg ccgatgtccg agcccacgcc
     2401 gacgaccaca ccggcgctga tgcggccttc gaccatcgac tcgccgagcg tgccggcgtt
     2461 gaagttgcag aagccctcgt gcatcacggt ggtgccctcg gcgaggtggg cgccgaggcg
     2521 gacgcggtcg gcgtcggcga tgcggacgcc gctgggcacc acgtagtcga ccatccgcgg
     2581 gaacttgtcg atcccgaaga cggtcacggt gtgcccggcc acgcgcagcc gcatccgggt
     2641 ctgctcgaac ccttcgacgg cgcagggacc gaggttggtc cacacgacgt tggtgagcag
     2701 gccgaagatg ccgtcgaggt tgcagccgtg gggcgggacc aggcggtgcg acagcaggtg
     2761 gagccgcaag tagcagtcgt gggtgtcgac cggggcgtcg tcgaggtcgc tgatgaacgt
     2821 caccaccgcg gcccgcttca cccggcggag gtcgtcggac tcagtcaggt gctggacatc
     2881 gacccgcagg tagtcgaggg cctggccccg gctcagcgga taggtgccgg agccgcccgt
     2941 gtgcccgtgc tcctctgcca ggcgcagcgc accctcggtg agcccgagga tggggtcggg
     3001 gaaccacgtg tcgagcacct tgccgtcggc gctgtaggtg gcgatgccca ccgcccacgc
     3061 ggcggaggtc ggagctgctg ggtcggtcac ggccgacacc gtaccggtcg ccgcgccgtc
     3121 gccccagcag gtgacgggcc tcaggcgacc tgccacgagc gcagcttggc ccacgctgct
     3181 tcgtccagcc gggccgtgag cgccatcccg ccctcgccga cctcctcgcg caggacctcg
     3241 ccctcgcggt ggacctcggc gagcacgtcg ccgcggtcga acgggatgag gaggtc
//
LOCUS       DATMFS010000176         6195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_123625, whole genome shotgun sequence.
ACCESSION   DATMFS010000176 DATMFS010000000
VERSION     DATMFS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123625"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VNQ33_03260"
     CDS             complement(<1..515)
                     /locus_tag="VNQ33_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97151.1"
                     /translation="MGDLGFWRTAEADPAHLALVGPDGTERTAGELLAASHRAANAFR
                     ALGLEGGDTVAVLLPNSIELIELYLGALEVGLYFTPINWHLVGPEVAYILQDSDAKVF
                     VASERFAEVARNAAAETGFPAEASFSLGAVDGFRPLAELYEGQSDDVPDKRSTGAAMH
                     YTSGTTGRPKGV"
     gene            complement(582..1580)
                     /locus_tag="VNQ33_03265"
     CDS             complement(582..1580)
                     /locus_tag="VNQ33_03265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97152.1"
                     /translation="MTVRFERVAGPLVPWPELDRRPGRTVFQTRAWLDFLIETQGAEP
                     VTARLFDDGEAAGWFTGAVVRRAGVRILGSPLKGWTTPAMGFDLPLGSDPRAVLDGLA
                     RFAFDDLRCLHLEVADRQVLDEAAAPDGFVVGRLPGYELALADDDTMLAGMTRGGRRD
                     VRRALRNGIVTTEVDPQDPGTFAASYYAQVTEAFAKRDRRPTYPQARVESLIRHLGPT
                     GRLVLLEARTPDGDLAATGIFPGMAGGTAEYWMGASWRRHQHLLPNEALMWHALRAWR
                     DRGAVRMNFGGGGSYKAKYGGSPHVLPWLRTSRFRVLEHARSVAAGRYGRSRRRPW"
     gene            complement(1600..2610)
                     /locus_tag="VNQ33_03270"
     CDS             complement(1600..2610)
                     /locus_tag="VNQ33_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97153.1"
                     /translation="MSTGFDVAPAWPNGARFAFSIFDDTDKTTLENGPPVYDLVTDLG
                     LRITKSVWPVAPDGPARTGGTTCDDPGYVAWVQQLQEVGHEIGFHNASDHPSVRATTH
                     RALDRFAELFGGPPRIGADHVGNHEAVYWGPKRLTGVRSQAYALGMQVTRPYRTTTSG
                     EVPDSEYFWGDLLRDRIDYWRNFTFRGIDTLRACPQLPYHDPARPYVNWWFASSHAPT
                     VDAFVAMFTPERLDELEESGGACIAYTHFGMRFAPDGTVDRRVAATLEDIARRNGWFA
                     PVSDVLDHIRAQRGDPGPITDAQRRSLEQRWVLEQLRSRAGTEARRVVARVRREPDPW
                     AP"
     gene            complement(2666..3739)
                     /locus_tag="VNQ33_03275"
     CDS             complement(2666..3739)
                     /locus_tag="VNQ33_03275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97154.1"
                     /translation="MTDGDTVFAHVEVGRARRTLRTTGVATAIHRPGSLPPTAWPTAS
                     ADRAAAARWSDRHPAVLTAARLELAEGAGSRAREALEAVGAHLVASGGRRLDVRLPAA
                     NPVADWLHGSGWMQGAVPGSVVISRSLRYPWSHPTPLGTAPSRSGGLPPSVRRLGRRL
                     ATMHPAQAPELIRIAAAEVAAAIRFRYAPGSLRVRPVDGAPPGTSPFAVTRYRTIRTA
                     LALVPPELRRTRLVDIGCGDGRVLREAIDRGWASVHGRELDPELGDRARTAIGSHGTV
                     DVGDGLAAPLPDDTGVVYLNNPFQVDGLIRLAGLLADSLTRRRRPLVVVYINPPSIEP
                     LAAEGFTLVQCTPAFSMLTFASG"
     gene            3946..5025
                     /locus_tag="VNQ33_03280"
     CDS             3946..5025
                     /locus_tag="VNQ33_03280"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWJ97155.1"
                     /translation="MRAALLHNTGDETLEIVDDIEVDAPGPGEVTIKIEATGVCHSDL
                     HAMAGSLPQPAPFVPGHEGAGVISAVGEGVTALAEGDHVVVAWSPPCGKCNNCVERKQ
                     PHLCVMIQFAIAGTPRFTRNGEPVFGMAGTGTFAEYLTVPQEAAVKIDDDIPFEIASL
                     IGCGVSTGVGAAINTAKVTPGSKVVVFGCGGVGIAAIQGAHVAGASVIVAVDLNEDKL
                     ELAKSFGATHGCKPDDLAALQAELTGDGFDFAFEAIGLPVTMRAAYDAVRRGGTACVI
                     GVGAMDKEVSFNGFEIFFSEKTFMGSYYGSVDVRSDFHRLLSLWKSGQLNLDGMITNR
                     MKIDEINDAFDIMKKGDAIRTVITF"
     gene            5120..5632
                     /locus_tag="VNQ33_03285"
     CDS             5120..5632
                     /locus_tag="VNQ33_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97156.1"
                     /translation="MGGAGEVWVGVTGHRDLARPDEVAAAVDAALDALAGDEGLPLVA
                     LSGLAEGADRIVAQRVLARPGGRLIALLPLEPADYVADFADVASVEEFTALLGAADEY
                     EVVRGAPSDDWTREAAYERAGHAMLDRADVLLALWDGEPGRGRGGTAEMVFEAGLIGR
                     RCEVVAVERA"
     gene            5632..>6195
                     /locus_tag="VNQ33_03290"
     CDS             5632..>6195
                     /locus_tag="VNQ33_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWJ97157.1"
                     /translation="MLNDSRRRWFVLAFLWVVIMAIGITGFVKQGSADDVDRVFLDNL
                     YLTFQLGTLDYDGSSGPMNWQVQLARFVVPLLAAGTILQTASVVFREEFRRYRLRYEK
                     GHTVICGLGETGTRLATAFAEAGAHVVAIEPDSAVAAGSGVAERLDHVLSGSGTDEEL
                     LRTAGIDRAARIVVCTGDDATNVQVTQV"
BASE COUNT          838 a         2221 c         2288 g          848 t
ORIGIN      
        1 acgcccttgg ggcggccggt ggtgcccgac gtgtagtgca tggcggcgcc ggtgctgcgc
       61 ttgtcgggca cgtcgtcgct ctggccttcg tacagctcgg cgagcggccg gaagccgtcg
      121 acggcgccga gggagaacga cgcctcggcg gggaagccgg tttcggcggc ggcgttgcgg
      181 gcgacctcgg cgaagcgctc ggaggccacg aacaccttgg cgtcgctgtc ttggaggatg
      241 tacgccacct cggggccgac gaggtgccag ttgatgggtg tgaagtacag gcccacctcg
      301 agggcgccga ggtacagctc gatcagctcg atcgagttcg gcaggagcac ggcgacggtg
      361 tcgccgccct cgagcccgag cgcgcggaac gcgttggcgg cccggtggct ggcggccagc
      421 agctcgccag cggtgcgctc cgtcccgtcg gggccgacca gcgcgaggtg cgcggggtcg
      481 gcttcggccg ttctccagaa tccaaggtca cccatggacg gggactgtag gagaaaataa
      541 gaacgcgttc tagttctggg cgctggggcg gcggctgcgg ctcaccacgg ccggcggcgg
      601 ctccgcccgt accgcccggc ggccaccgac cgggcgtgct cgagcacgcg gaaccgggag
      661 gtgcgcagcc acgggagcac gtgcggcgag ccgccgtact tcgccttgta ggagccgccg
      721 ccgccgaagt tcatccggac cgcgccccgg tcccgccacg cccgcagggc gtgccacatg
      781 agcgcctcgt tcggcagcag gtgctggtgg cgccgccagc tggcacccat ccagtactcg
      841 gcggtccccc cggccatccc cgggaagatg ccggtggccg ccaggtcgcc gtcgggggtg
      901 cgggcctcca gcagcaccag gcggccggtc ggccccaggt ggcggatcag gctctcgacg
      961 cgggcctggg ggtaggtggg gcgccggtcg cgcttggcga acgcctcggt cacctgggcg
     1021 tagtaggacg cagcgaaggt gccgggatcc tgcgggtcga cctcggtcgt gacgatcccg
     1081 ttccgcaggg cccggcggac gtcgcggcgg ccgcctctgg tcatcccggc gagcatcgtg
     1141 tcgtcatcgg cgagcgccag ctcgtagccc ggcagccggc cgaccacgaa gccgtccggc
     1201 gcggcggcct cgtcgaggac ctggcggtcg gccacctcca ggtggaggca ccgcaggtcg
     1261 tcgaaggcga accgggccag cccgtcgagc accgcccggg ggtccgatcc cagcggcagg
     1321 tcgaagccca tcgccggcgt cgtccacccc ttcagcgggc tgcccaggat ccggactccc
     1381 gcccgccgga cgacggcgcc ggtgaaccac ccggccgcct cgccgtcgtc gaagagccgt
     1441 gccgtcaccg gctccgcccc ctgggtctcg atcaggaagt ccagccaggc ccgtgtctgg
     1501 aacaccgtcc gcccggggcg gcggtccagc tcgggccacg gcaccagcgg cccggcgacg
     1561 cgctcgaagc ggacggtcat cgggcggctc ggtccacggt cacggtgccc acggatccgg
     1621 ctcgcgccgc acccgcgcca cgacgcggcg ggcctccgtc ccggctcggg agcggagctg
     1681 ctccagcacc caccgctgct ccaacgaccg gcgctgcgcg tcggtgatcg gccctgggtc
     1741 tccccgctgg gcgcggatgt ggtcgagcac gtcggacacg ggggcgaacc agccgttgcg
     1801 ccgggcgatg tcctcgagcg tggcggcgac ccggcggtcg acggtcccgt ctggcgcgaa
     1861 gcgcatcccg aagtgggtgt aggcgatgca cgccccgccc gactcctcga gctcgtcgag
     1921 gcgctccggg gtgaacatgg cgacgaacgc gtcgacggtg ggggcgtggc tcgaggcgaa
     1981 ccaccagttc acgtacggcc gggccgggtc gtggtacggc agctgggggc aggctcggag
     2041 cgtgtcgatg ccgcggaagg tgaagttccg ccagtagtcg atgcggtcgc gcagcaggtc
     2101 gccccagaag tactcgctgt cggggacctc gcccgaggtg gtcgtccggt agggccgggt
     2161 gacctgcatg cccagcgcgt aggcctggct gcggacgccg gtgagccgct tcggccccca
     2221 gtacacggcc tcgtggttgc cgacgtggtc ggcaccgatc cgaggcggtc caccgaacag
     2281 ctcggcgaac cggtcgagcg cccgatgggt ggtggcccgg accgaagggt ggtcgctggc
     2341 gttgtggaag ccgatctcgt ggccgacctc ctggagctgc tgcacccagg cgacgtagcc
     2401 ggggtcgtcg caggtggtgc ccccggtgcg ggcgggtccg tcgggcgcca ccggccagac
     2461 ggacttggtg atccgcagcc cgaggtcggt gacgaggtcg tagaccggtg gtccgttctc
     2521 cagcgtcgtc ttgtcggtgt cgtcgaagat cgagaaggcg aaccgcgccc cgttcggcca
     2581 cgccggcgcg acatcgaacc cggtgctcac ggtggcctct cctccctcac gacccgctcc
     2641 ccgggtcacg gtaccggctc cggcctcaac ctgaggcgaa cgtcagcatc gagaacgccg
     2701 gggtgcactg gacgagcgtg aacccctcgg cggcgagcgg ctcgatgctg ggcgggttga
     2761 tgtagacgac cacgagcggc cggcgacggc gggtgagcga gtcggcgagc aggcccgcca
     2821 ggcggatgag cccgtcgacc tggaacgggt tgttcaggta gacgaccccg gtgtcgtcgg
     2881 gcagcggggc ggccaagccg tcgcccacgt cgaccgtgcc gtggctgccg atggcggttc
     2941 gggcccggtc gcccagctcg ggatccagct cccggccgtg gaccgaggcc catccccggt
     3001 cgatcgcctc gcgcagcacc cggccgtcac cgcagccgat gtcgacgagg cgagtccgcc
     3061 gcagctccgg tggcacgagc gccagtgccg tgcggatcgt ccggtaccgg gtcaccgcga
     3121 agggggaggt ccccggcgga gctccgtcga ccggccgcac gcgcagcgag cccggggcgt
     3181 agcggaagcg gatcgccgcc gccacctccg cggcagcgat gcggatcagc tcgggcgcct
     3241 gggccgggtg catggtcgcc agccgtcgcc ccaggcggcg caccgacggg gggaggccgc
     3301 cggaccggga cggcgccgtg cccagcggcg tcgggtggct ccacgggtac cgcagcgacc
     3361 ggctgatcac caccgatccc ggcacggcgc cttgcatcca gccggacccg tgcagccagt
     3421 cggcgacggg attggctgcc ggcagccgca cgtcgaggcg gcggccgccc gatgccacga
     3481 ggtgcgcgcc gacggcctcc agcgcctccc gggctcggct ccccgcgccc tcggcgagct
     3541 cgaggcgcgc cgccgtcagc accgccgggt ggcggtccga ccagcgggcg gcggccgctc
     3601 ggtccgccga tgcggtgggc caagcggtgg gcgggaggga gccgggccgg tggatggccg
     3661 tggccacccc cgtggtccgc agcgtgcggc gcgcccgccc gacctcgacg tgggcgaaca
     3721 cggtgtcccc atcggtcacg gcgtagcgct ggacgctccg gcgcggggtg ccgacgagct
     3781 ggacgtgtcg tgcccttgct tcgcccacgt gtgcaatgcc tccctcaccg atcgggcctg
     3841 gagcctaggc agccacgtcc ccgccggggc cgtggtcaga actggaacgc gttctactaa
     3901 ggtccgcgga gcaaggtccc aggggaactc caaggagcaa cgaacatgcg cgctgcgctg
     3961 ctgcacaaca cgggcgacga gacgctcgag atcgtcgacg acatcgaggt cgacgcaccc
     4021 ggccccggcg aggtcaccat caagatcgaa gccacgggcg tctgccactc ggacctccac
     4081 gccatggctg gctcgctccc gcagccggcg ccgttcgtgc ccggccacga gggtgccggc
     4141 gtcatctccg ccgtcggcga aggcgtcacc gcgctcgccg agggggacca cgtcgtcgtc
     4201 gcctggagcc cgccctgcgg caagtgcaac aactgcgtcg agcgcaagca gccgcacctg
     4261 tgcgtgatga tccagttcgc catcgccggc accccgcgct tcacccgcaa cggcgagccc
     4321 gtcttcggca tggccggcac cggcaccttc gccgagtacc tcaccgtccc gcaggaagcc
     4381 gccgtcaaga tcgacgacga catccccttc gagatcgcct cgctcatcgg ctgcggcgtg
     4441 tccaccggcg tgggagccgc catcaacacc gccaaggtca cccccggctc caaggtcgtg
     4501 gtgttcggct gcggcggcgt cggcatcgcc gccatccagg gcgcccacgt ggccggcgcg
     4561 tcggtcatcg tcgccgtcga cctcaacgag gacaagctgg agctggccaa gtcgttcggt
     4621 gccacgcacg gctgcaagcc cgacgacctc gccgccctcc aggccgagct caccggcgac
     4681 ggcttcgact tcgccttcga ggccatcggc ctcccggtca ccatgcgcgc cgcctacgac
     4741 gccgtgcgcc gcggcggcac cgcctgcgtg atcggcgtgg gcgcgatgga caaggaggtc
     4801 tcgttcaacg gcttcgagat cttcttctcg gagaagacgt tcatgggcag ctactacggc
     4861 tcggtcgatg tccgctcgga cttccaccgg ctcctcagcc tctggaagag cggccagctc
     4921 aacctcgacg gcatgatcac caaccggatg aagatcgacg agatcaacga cgccttcgac
     4981 atcatgaaga agggcgacgc catccgcacg gtcatcacgt tctgacctcg cttcgcatcg
     5041 gacggatctg aagcagccgg ggccttcggg ccccggctgc tccgcgtctc aggccggctc
     5101 cgcctggcat gatgcgctgg tgggtggtgc gggagaggtc tgggtcggcg tgacggggca
     5161 ccgggacctg gcgcgccccg acgaggtggc cgccgcggtc gacgccgcgc tggatgcgct
     5221 cgccggcgac gaggggctgc cgctcgtggc cctctccggt ctggcggagg gtgccgaccg
     5281 gatcgtggcc cagcgggtgc tggcccggcc cggtggccgg ctgatcgcgc tccttcccct
     5341 ggagccggcc gactacgtgg cggacttcgc cgacgtcgcc tcggtcgagg agttcaccgc
     5401 gctgcttggt gcggccgacg agtacgaggt ggtccgagga gcaccgagcg acgactggac
     5461 ccgggaggcc gcctacgagc gcgccggcca cgccatgctc gaccgggccg acgtgctgct
     5521 cgcgctgtgg gacggcgagc cgggccgggg ccgaggcggc accgcggaga tggtgttcga
     5581 ggccgggttg atcggccgcc gctgcgaggt cgtcgcggtg gagcgggcct gatgctgaac
     5641 gacagcagga ggcgctggtt cgtcctggcg ttcctgtggg tcgtcatcat ggcgatcggc
     5701 atcaccggct tcgtgaagca gggctcggcc gacgacgtgg accgggtgtt cctcgacaac
     5761 ctctacctca cgttccagct cggcaccctg gactacgacg gcagctccgg tccgatgaac
     5821 tggcaggtcc agctggcccg gttcgtcgtc ccgctcctcg ccgccgggac gatcctgcag
     5881 acggcgtcgg tggtgttccg cgaggagttc cgccgctacc gcctccgcta cgagaagggc
     5941 cacacggtca tctgcggcct gggcgagacg ggtacccggc tcgccactgc cttcgccgag
     6001 gccggcgccc acgtggtggc gatcgagccc gactcggccg tcgctgccgg ctcgggcgtc
     6061 gccgagcggc tcgaccacgt gctgtcgggc agcggcaccg acgaggagct gctgcgcacg
     6121 gccggcatcg accgagccgc ccggatcgtg gtctgcaccg gcgatgacgc cacgaacgtg
     6181 caggtcaccc aggtc
//
LOCUS       DATMFS010000177         5535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_123665, whole genome shotgun sequence.
ACCESSION   DATMFS010000177 DATMFS010000000
VERSION     DATMFS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123665"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VNQ33_03295"
     CDS             <1..809
                     /locus_tag="VNQ33_03295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ97158.1"
                     /translation="GLADACDQVGVRIIEHTPVTGLERRPEGGIRLVAARGTVDARQV
                     ALGTNVFPSLIKRTRLLTVPVYDHVLVTEPLSSQQRDAIGWSERQGIGDSANQFHYYR
                     QTADHRILWGGYDAVYHWGRRVKDRYDHRPATSQRLAEHFFATFPQLEGLRFTHAWGG
                     PIDTCTRFCAFFGTAHDGQVAYAAGYTGLGVGATRFGADVMLDLLAGEETERTRLAMV
                     RSTPIPFPPEPAAWLGITATRWSLAQADRNEGRRNLWLRTLDRLGLGFDS"
     gene            908..1285
                     /locus_tag="VNQ33_03300"
     CDS             908..1285
                     /locus_tag="VNQ33_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97159.1"
                     /translation="MQSEQFIAKGIAIGRVAYGVACMAAPRAMLGPSGPRAEGSSVMM
                     ARFFGVRDLVLGGGTLLALQRDPDAAVSWVEWSAAADALDVAVGIASHRDLDRAGRFG
                     VAGIALPAALGGAWAARRLRAAR"
     gene            complement(1292..2044)
                     /locus_tag="VNQ33_03305"
     CDS             complement(1292..2044)
                     /locus_tag="VNQ33_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97160.1"
                     /translation="MTRTTHRIFLSAMAASFALVISACGASGGSSDGGKDTTTTTTKA
                     PAESTTTTTADDAEAAQDRADSVDVELSDFPEGWDSAPADDPDAESPLNDCDPALGDR
                     STQLARHATDDFSIGSFERGDGVQFTARTVVFEDEAAAEAAVAPFSDSKVLSCIDDAL
                     KSAYTSAGEGITVEGSLEQGDADFGADETVALSATYTVSAEDGSSMDVNLGVLVMRTG
                     DVATNIVVQTVDPDFDPSVLPVEKLADQLNAA"
     gene            complement(2218..2904)
                     /locus_tag="VNQ33_03310"
     CDS             complement(2218..2904)
                     /locus_tag="VNQ33_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97161.1"
                     /translation="MEPADDARRDWLRVDAADVDWVVEGSFLRSTWQCIWGRGCLGIL
                     DEPAEELGQGCCSVGAHLDGEDEARTTATLAAMLDPVRFQFHAEAADGVFSDETATNT
                     RVVDGACIFLNRPGFAGGEGCALHLGAIDDGEPPMDWKPSVCWQLPIRVDWEPIDGGR
                     ERATVRRWSRADWGDEGKDMAWCCTEGDRAYGADRPVIESLADELTGVMGAEAVEELR
                     RQLAEPPEAR"
     gene            2987..3466
                     /locus_tag="VNQ33_03315"
     CDS             2987..3466
                     /locus_tag="VNQ33_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97162.1"
                     /translation="MPHPFRSLVVTGSLLVLATGAAACGSSGGSDASASTTAAKATTT
                     SHATTTTEASTTGSSSGETPTTTGQVVDPATGEVDCDALLQEYTVVFDPDDLASSVAF
                     FRKYEPSMPDDVAAASERLAAAYEKAGDLGHMDMGSLDLTADAQAFTDWLDNGCPAG"
     gene            3492..4895
                     /locus_tag="VNQ33_03320"
     CDS             3492..4895
                     /locus_tag="VNQ33_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWJ97163.1"
                     /translation="MRASIDRFVSFIARVITRGLYRSVEVIGFDDLPSGPRLIVANHF
                     NGFVDPVVLAGALGRLPRFIAKATLWKTPGVPVLMRVVGVLPVHRTADGGGGDNARTF
                     VEVVRELHEGMTVAIFPEGTTHDNQHLSPVRTGAARLALDAAHDRVPGMHIVPVGVTF
                     EDKVALRSRVVIRAGTPIDPAGLAYLDAEGEPIGSDDHDAVRRLTEEIRSGLAAAAPD
                     FETLLDAVEMRRAADVALRTHGVAPLAEVSLARREQLAKELDRLPEDRREPIARAAAE
                     YELLLGAVRVTDRYLMPRVLVKDLVKRLVITAVFVAVLAPFALAGIVVNLIPTLLVVL
                     AGLAAKAPVSKGTNRVLVGFVAFPVMWLSFAVWDVGGSFMSDALSLATWPLSPEIDWI
                     FGSRGGWGASLLVFVACPVFGLLAVWLAEQITRLYRTWRAVSTSLNRRGQLRDLLERR
                     DALVTMVGDAHGSAVGS"
     gene            5001..>5535
                     /locus_tag="VNQ33_03325"
     CDS             5001..>5535
                     /locus_tag="VNQ33_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97164.1"
                     /translation="MTTGEAGIGRAATGTDQAPLALVRQAGSVLLGSPEPIVVAVDAF
                     LARGHVLFEDVPGVGKTLLAKALARSIGGTFGRVQGTPDLLPSDLTGISHLDEDRRSW
                     VFRPGPLFNNIVLVDEINRATPRTQSALLEAMAEGQVTVDGTTHPLPEPFLVIATQNP
                     QNDDPGTFPLVAGQRDRF"
BASE COUNT          708 a         2081 c         1956 g          790 t
ORIGIN      
        1 gggggctggc ggatgcgtgc gaccaggtcg gggtgcggat catcgagcac acgccggtca
       61 ccggcctcga gcgccggccc gagggcggga tccgcctggt ggcggcgcgc gggaccgtcg
      121 acgcccgcca ggtggcgctc ggcaccaacg tgttcccgtc gctcatcaag cgcacgcgcc
      181 tgctcaccgt gcccgtctac gaccacgtgc tggtcaccga gccgctctcc tcccagcagc
      241 gcgacgccat cggctggtcc gagcgccagg gcatcggtga cagcgccaac cagttccact
      301 actaccgcca gacggctgac caccggatcc tgtggggcgg ctacgacgcg gtctaccact
      361 ggggccgacg ggtgaaggac cgctacgacc accgccccgc cacgtcccag cggctcgccg
      421 agcacttctt cgccaccttc ccccagctcg aggggctgcg gttcacccac gcgtggggcg
      481 gtccgatcga cacgtgcacc cggttctgcg cgttcttcgg gaccgcgcac gacgggcagg
      541 tcgcctacgc cgccgggtac accggcctcg gcgtgggcgc cacccgcttc ggcgccgacg
      601 tgatgctgga cctcctcgcc ggcgaggaga ccgagcgcac ccgcctggcg atggtccgct
      661 ccacgcccat cccgttccca cccgagccgg ccgcgtggct cggcatcacc gccacccgct
      721 ggtcgctcgc ccaggccgac cgcaacgagg gccgccgcaa cctctggctg cgcacgctcg
      781 accgcctcgg cctcggcttc gactcctgac cgaccccgac ctgatcctgg gcttcgtggg
      841 cgaggccccc catcgaggcg gctggcccac gagctgcgat cggggtgcgg tgtgcgatgc
      901 tcggccgatg cagagcgaac agttcatcgc caaggggatc gcgatcgggc gggtggccta
      961 cggcgtggcg tgcatggccg cgccgcgggc gatgctcggc ccgtcagggc cacgagccga
     1021 gggctcgtcg gtgatgatgg cccggttctt cggcgtgcgc gacctggtgc tcgggggcgg
     1081 cacgctcctc gccctccagc gcgaccccga tgccgcggtc agctgggtcg agtggagcgc
     1141 ggcggccgac gcgctcgacg tcgccgtcgg catcgcatcg caccgggacc tcgaccgagc
     1201 gggccggttc ggcgtcgccg gcatcgccct gcccgccgcg ctcggcggtg cctgggcggc
     1261 gcgccgcctg cgcgccgccc gctgaccggg gtcaggcggc gttgagctga tcggccagct
     1321 tctcgacagg caggacgctc gggtcgaagt cggggtcgac cgtctggacg acgatgttgg
     1381 tggcgacgtc gccggtgcgc atcacgagca cgccgaggtt cacgtccatc gacgagccgt
     1441 cctcggccga gacggtgtag gtggcgctga gggcgacggt ctcgtcggcc ccgaagtcgg
     1501 cgtcgccctg ctccaggctg ccttcgacgg tgatcccctc gccggccgac gtgtacgccg
     1561 acttgagcgc gtcgtcgatg cagctcagga ccttggagtc gctgaacggg gccacggccg
     1621 cctcggccgc cgcctcgtcc tcgaacacca cggtgcgggc ggtgaactgc acgccgtcgc
     1681 cgcgttcgaa cgagccgatc gagaagtcgt cggtggcatg gcgcgccagc tgcgtggagc
     1741 ggtcgccgag ggccgggtcg cagtcgttca gcgggctctc ggcgtcgggg tcgtcggccg
     1801 gcgccgagtc ccagccctcg gggaagtcgg agagctcgac gtcgacggag tcggcccgat
     1861 cctgggctgc ctcggcgtca tcggccgtgg tcgtggtggt cgactcggcc ggcgccttcg
     1921 tggtggtggt ggtggtgtcc ttgccgccgt cggacgagcc gccggaggct ccgcaggccg
     1981 agatcaccag ggcgaacgag gccgccatgg cgctgaggaa gatccgatgg gtggtgcgcg
     2041 tcacggggtg ctcctgctcg aggtgggtgg tccgccgggt cgcaaccatg ttgccccacg
     2101 ccggccgccg agctgggcaa aagtgacccg cgggagcccg gcccgcggcc tcgcacgacc
     2161 tggcgtgcgc gcgacgtgag ggagatcagc cgccacctcg acgggacgtg ccggggttca
     2221 gcgggcctcc ggaggctccg ccagctggcg ccgcagctcc tccaccgcct cggcgcccat
     2281 cacccccgtc agttcgtcgg cgagcgactc gatgacgggc cggtcggcgc cgtaggcccg
     2341 gtcgccctcg gtgcagcacc aggccatgtc cttgccctcg tcgccccagt cagcgcgcga
     2401 ccagcgtcgg accgtcgccc gctcccggcc accgtcgatc ggctcccagt ccacgcggat
     2461 ggggagctgc cagcagacgg agggcttcca gtccatcggc ggttcgccgt cgtcgatcgc
     2521 accgaggtgc agggcgcagc cctcgccgcc ggcgaagccc ggacggttga ggaagatgca
     2581 ggcgccgtcg acgacccgcg tgttcgtcgc ggtctcgtcg ctgaagaccc cgtcggcagc
     2641 ctcggcgtgg aactggaacc gaaccgggtc caacatcgcg gcgagcgtcg ccgtggtgcg
     2701 cgcctcgtcc tcgccgtcga ggtgcgcacc gaccgagcag cagccttggc cgagctcctc
     2761 ggccggctcg tcgaggatgc cgaggcagcc cctcccccag atgcactgcc aggtcgagcg
     2821 gaggaacgac ccctccacga cccagtcgac gtcggcggca tcgacccgca gccagtcgcg
     2881 tcgtgcgtcg tcggcgggtt ccatggtggg aagcctggca cggctggggg tcctagcctg
     2941 atgccgctcc gacccgaccc cacccgcccc gcccaggacg tgcgccatgc cccacccctt
     3001 ccgctccctc gtggtcaccg gctcgctgct cgtcttggcc acgggcgcgg cggcctgtgg
     3061 ctcatctggc gggagcgacg catcggcgag caccacggcg gcgaaggcca ccacgacctc
     3121 gcacgcgacc accaccaccg aggcgagcac caccggcagc agcagcggcg agaccccgac
     3181 gaccacgggc caggtcgtgg acccggccac gggcgaggtc gactgcgacg cgctgctgca
     3241 ggagtacacg gtcgtgttcg accccgatga cctggcctcc agcgtcgcct tcttccggaa
     3301 gtacgagcct tcgatgcccg acgacgtcgc cgccgcatcc gagcggctgg cggcggccta
     3361 cgagaaggcc ggcgacctcg gccacatgga catgggctcc ctcgacctca ccgccgacgc
     3421 ccaggccttc acggactggc tcgacaacgg gtgccccgcc gggtgagccc gtctcgcgag
     3481 acggccggct gatgcgggcg tcgatcgacc ggttcgtctc gttcatcgcc cgcgtcatca
     3541 cacggggcct ctaccgctcg gtcgaggtga tcgggttcga cgacctgccc agcggtccgc
     3601 gcctcatcgt cgccaaccac ttcaacgggt tcgtcgaccc ggtcgtgctg gcgggggcgc
     3661 tcgggcggct gccgcggttc atcgccaagg ccaccctctg gaagacgccg ggcgtgccgg
     3721 tgctgatgcg ggtcgtcggc gtgctgcccg tgcaccggac ggcggacggc ggcggcggcg
     3781 acaacgcccg cacgttcgtc gaggtcgtac gggagctgca cgagggcatg acggtggcga
     3841 tcttcccgga gggcaccacg cacgacaacc agcacctgtc gccggtgcgc accggggccg
     3901 cacgcctcgc gctcgacgcc gcgcacgacc gcgtgccggg gatgcacatc gtccccgtcg
     3961 gcgtcacgtt cgaggacaag gtcgccctgc gctcccgcgt cgtcatccgc gccggtacgc
     4021 cgatcgaccc ggccggcctc gcctacctcg atgcggaggg cgagcccatc gggtccgacg
     4081 accacgacgc cgtgcgccgc ctcaccgagg agatccgatc gggcctggcg gccgccgcgc
     4141 ccgacttcga gaccctgctc gacgccgtcg agatgcggcg agccgccgac gtggccctgc
     4201 gcacgcacgg cgtcgcgccg ttggcggagg tgtccctcgc ccgccgtgag caactggcca
     4261 aggagctgga ccgcctgccc gaggaccggc gcgagcccat cgcccgagcc gccgccgagt
     4321 acgagctcct gctgggcgcg gtgcgcgtca cggatcggta cctgatgccg cgggtcctcg
     4381 tgaaggacct ggtgaagcgg ctcgtgatca ccgccgtctt cgtggcggtg ctggccccgt
     4441 tcgcgctggc cggcatcgtc gtcaacctga tccccaccct gctcgtggtc ctcgccgggc
     4501 tggccgccaa ggcgccggtg tcgaagggga ccaaccgggt cctggtcggg ttcgtcgcct
     4561 tcccggtgat gtggctctcg ttcgcggtgt gggacgtcgg aggctcgttc atgtccgacg
     4621 cgctgagcct ggccacctgg ccgctgtcgc ccgagatcga ctggatcttc gggtcgcgcg
     4681 gcgggtgggg agcgtccctg ctcgtgttcg tcgcctgccc ggtgttcggg ctgctcgccg
     4741 tgtggctggc cgagcagatc acccgcctct accggacgtg gcgcgccgtg agcaccagcc
     4801 tcaaccgccg cggccagctc cgggacctgc tcgagcggcg cgacgcgctg gtgacgatgg
     4861 tcggcgacgc ccacgggtcc gccgtcggct cctgaccacc ccgctccacc gggctcggcc
     4921 gccgacccgc cgcgatcgtc gggtcatcac cacgctgtgc aacactcgcg ccacgctggc
     4981 ggatcagggg gacgggcacg ttgaccacag gtgaagcggg gatcgggcga gcggcgaccg
     5041 gcaccgacca ggcgccgctg gccctcgtcc gccaagccgg ctcggtgctg ctcggcagtc
     5101 ccgagcccat cgtcgtggcc gtcgacgcgt tcctcgcacg ggggcacgtc ttgttcgagg
     5161 acgtacccgg tgtcggcaag acgctgctcg cgaaggcgct cgcccgctcg atcggcggca
     5221 cgttcggccg agtccagggc acgcccgacc tgctgccctc ggacctcacc gggatctccc
     5281 acctcgacga ggaccggcgg tcttgggtgt tccgccccgg cccgctgttc aacaacatcg
     5341 tcctcgtgga cgagatcaac cgggcgacgc cccgcaccca gtcggcgctg ctcgaggcca
     5401 tggccgaagg ccaggtcacc gtcgacggga ccacgcaccc gctgcccgag ccgttcctcg
     5461 tcatcgccac ccagaacccg cagaacgacg atccgggcac gttccccctg gtggccggcc
     5521 agcgcgaccg ctttg
//
LOCUS       DATMFS010000178         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193652, whole genome shotgun sequence.
ACCESSION   DATMFS010000178 DATMFS010000000
VERSION     DATMFS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193652"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VNQ33_03330"
     CDS             complement(<1..447)
                     /locus_tag="VNQ33_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ97165.1"
                     /translation="MTDPLRVILANDAELIIEGLKGVLAPYPERVQVVATAQGDPDIV
                     QEALVETDADVMLIDSFGRTGAGIDAVAKVLATDPSFAVVVFTEADDLRHLFSALRAG
                     VRGYLLKTISSDELVASLERIAGGEVVVDAGLATEAAIMAARTSSRQ"
     gene            complement(597..1235)
                     /locus_tag="VNQ33_03335"
     CDS             complement(597..1235)
                     /locus_tag="VNQ33_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97166.1"
                     /translation="MFLESVEPHFSMFTLSGFPSFAATERLEAQMLAEADLPLLAPPS
                     SGRVSPTTFHVALADGEPLGLAASTVGPLAELPLGLGLRAAGVDVETEVDLAGPACEL
                     VSLSVDPDADRDTTGVTEALYRSFYRHAKRSGALSLVVGVDPWIFDTLTEQYGVPFAV
                     LGPTLDLLGRELLAIGGEITALEEGVRRNAPGFFAYLSRPESGAAAVGAVPI"
     gene            complement(1320..2315)
                     /locus_tag="VNQ33_03340"
     CDS             complement(1320..2315)
                     /locus_tag="VNQ33_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97167.1"
                     /translation="MTTRWRALLASALLVALAACSGGSGDAGAPTATAAPEATATTAP
                     APEGTAGCGREPDVARLGDEAPGDVAATFTSGGVERVYRLSVPPDYEPDEPAPLLFNL
                     HGSGSNAIQASVYGDVPRRAAEGGMIVVTPEAVAGQWQLGGTGTDGDFLIGLLDDVEA
                     RYCIDQARTHIMGMSLGAWKAAATACSFPGRFASAALVTVEVFPGECEPLPVIAFHGT
                     GDHVVPYGEGADPGITITGFNANLPGAVSNIERWAANGGCDPEPEISEIGDDVVRRRF
                     GGCDPGIGVELYTVKGGDHTWPGADVEIAPPDGTTQTIDATDLALAWFADHPARG"
BASE COUNT          342 a          872 c          895 g          326 t
ORIGIN      
        1 ctgccgggag gaggtgcgag ccgccatgat cgccgcctcg gtggccaggc cggcgtcgac
       61 cacgacctcc ccgcccgcga tgcgctcgag ggacgcgacc aactcgtcgg aggagatggt
      121 cttgaggagg tagccgcgca ccccggcgcg cagggcggag aacaggtggc ggaggtcgtc
      181 cgcttcggtg aacaccacga cggcgaacga cggatcggtg gcgaggacct tggccaccgc
      241 gtcgatgccc gcccccgtcc gtccgaagga gtcgatcaac atgacgtcgg cgtcggtctc
      301 gaccagcgcc tcctgcacga tgtccggatc gccctgggcc gtggcgacga cctggacccg
      361 ctcggggtac ggagcgagca cgcccttcag tccttcgatg atgagctctg cgtcgttggc
      421 gaggatcacg cgcaggggat cggtcatggt gctgggtcct tcaagacgag ggttccggtg
      481 gtgcggagga tggtctcggc cacgggcgga ctcccacgga gcaagctcgg gcccgcggcc
      541 cttcctaccc tccgcctggg tgggcgatac ccggtccacc ggcggcggca gcatcgtcag
      601 atgggcacgg caccgaccgc ggccgcgccg gactccgggc gggagaggta ggcgaagaag
      661 ccgggagcgt tgcgacggac cccctcctcg agggcggtga tctcaccgcc gatcgccagg
      721 agctcgcgtc cgagcaggtc cagcgtcggg cccaggacgg cgaacggcac gccgtactgc
      781 tcggtcagcg tgtcgaagat ccacggatcc acgccgacca ccaggctgag ggcgcccgac
      841 cgcttggcgt gacggtagaa cgaccggtac agcgcttcgg tcacccccgt ggtgtcacgg
      901 tccgcgtcgg gatccaccga gagcgagacg agctcgcacg ccggcccggc caggtcgacc
      961 tcggtctcga cgtccacccc cgccgctcgc agcccgagac cgagcggcag ctcggccaac
     1021 ggccccaccg tcgacgccgc gagcccgagc ggctccccgt cggccagcgc cacgtggaac
     1081 gtggtgggcg acacccgccc gctcgacggc ggcgcgagca ggggcagatc ggcctcggcc
     1141 agcatctgcg cctccaaccg ctccgtggcc gcgaacgagg ggaacccgct cagggtgaac
     1201 atcgagaagt gcggctccac ggattcgagg aacatcggac gggcctcccg ggcacggaca
     1261 gaacgccgcg ccagtacccg cgcctccccc gatccaaccc ggtccggcga ccgcaggcgt
     1321 cagccgcgcg ccgggtggtc ggcgaaccac gcgagcgcca ggtccgtggc gtcgatcgtc
     1381 tgcgtggtgc cgtcgggcgg ggcgatctcg acgtcggccc cgggccaggt gtggtcgccg
     1441 cccttcaccg tgtagagctc gaccccgatg ccggggtcgc agccgccgaa cctccggcgc
     1501 acgacgtcgt cgccgatctc ggagatctcg ggctccggat cgcagccgcc gttcgccgcc
     1561 cacctctcga tgttggagac ggcgccgggg aggttggcgt tgaagccggt gatggtgatg
     1621 ccgggatcgg ccccctcgcc gtagggcacg acgtggtcgc cggtgccgtg gaaggcgatg
     1681 accgggaggg gttcgcactc gccggggaac acctcgacgg tgacgagggc ggccgacgcg
     1741 aagcgtccgg ggaacgagca ggcggtcgcc gccgccttcc aggcgcccag cgacatgccc
     1801 atgatgtggg tccgggcctg gtcgatgcag tagcgggctt cgacatcgtc gaggaggccg
     1861 atcaggaagt cgccgtcggt cccggtgccg ccgagctgcc actggccggc gacggcctcg
     1921 ggcgtcacga cgatcatccc gccttcggcc gcccgccggg ggacgtcgcc gtacacgctg
     1981 gcctggatgg cgttcgagcc gctgccgtgg aggttgaaca gcagcggtgc cggctcgtcg
     2041 ggttcgtagt ccggcgggac cgacaaccgg tagacccgct cgacgccgcc gctggtgaac
     2101 gtggcggcca cgtcgccggg ggcctcatcg ccgagccggg cgacgtcggg ctccctgccg
     2161 cagccggctg tgccctcagg cgccggtgcg gtggtggccg tcgcctcggg agcggccgtg
     2221 gcggtgggcg cgcccgcgtc accgctgcca cccgagcacg ccgccagcgc gacgagcagg
     2281 gccgacgcga gcagcgcccg ccaccgggtg gtcactcggc ccgcgcgtcc ttctcgatgg
     2341 cggcggcgtc ggccttgagc tgcttggcct cgatcttctc cgccttcttc atggcgtggg
     2401 tgatcttggc ctccatgtcc gacacaccga cgacg
//
LOCUS       DATMFS010000179         2905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_286306, whole genome shotgun sequence.
ACCESSION   DATMFS010000179 DATMFS010000000
VERSION     DATMFS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286306"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..668)
                     /locus_tag="VNQ33_03345"
     CDS             complement(204..668)
                     /locus_tag="VNQ33_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ97168.1"
                     /translation="MASWDEFARRAPDVARAGERLLTQFGPGLGFLATVDRAGAPRLH
                     PICPVVADGELWAFVITASPKCADLRRDGRYALHSFPPEEVDDEFVVRGAAAEASDLA
                     FGDRQWERIRAATTAAVGREHETPFRLGIDRAMVAAYEARGVFPPTYSTWRA"
     gene            complement(692..2125)
                     /gene="miaB"
                     /locus_tag="VNQ33_03350"
     CDS             complement(692..2125)
                     /gene="miaB"
                     /locus_tag="VNQ33_03350"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWJ97169.1"
                     /translation="MTDAAPDPKRYLVRTFGCQMNEHDSERIAGLFDADGMEATDDLE
                     QADVVVLNTCCIRENADNKLYGTLGHLKSLKDRRPGMEIVVSGCLAQKDKEGIRERAP
                     YVDVVFGTHNVHRATELLHRSREQGPLTEILEATVADDHELFPSAMPARRDEGYTAWV
                     TIQIGCDNSCAFCIVPAVRGEEISRPFGRLVDEVRDLAATGVTEVTLLGQNVNSYGRD
                     LMLAARQAGEDVRVRPLFADLLREVGAVDGIRRVRYTSPHPKDLRAETIAAMAETPEV
                     CEHLHLPVQSGSDRVLAAMHRGYTAERYLEKLAAARAAVPDLAVTTDLIVGFPGETDD
                     DFERTLELAAAAEYDSAYTFIYSPRPGTEAAEMVDQFVDPARVAERFDRLKAVLERSA
                     LRRHEARIGRVEEVVVEGPSRKDPSVTTGRTRQNKLVHFASGSPLRVGTYAEVLVTGA
                     APHFLRGDLVGVTAAARHRTRIPVTAG"
     gene            2304..>2905
                     /locus_tag="VNQ33_03355"
     CDS             2304..>2905
                     /locus_tag="VNQ33_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97170.1"
                     /translation="MDVIDKVRATLAATTGAITLVVYHALRSRDIGSGAAVLIALAAS
                     ICAALAIDALLFGYGLRFRSARKMLLKTGKYEGTWFVEHDDLDQHSLATISSVDGVVS
                     YQGIGVETTSGRICVVWQSTDIAFSIESGRFRYAFEARVQGEPYRVGEGPASQSRLLR
                     GYGILAFNEEVRGEPHTGLDTYIDIDSDPSVESMKASEHWG"
BASE COUNT          439 a          982 c         1053 g          431 t
ORIGIN      
        1 agcgccagcg acgacttgcc gcttgccgtg gtgcccacga ccgccagcgg cggcggcgac
       61 ccctccggca ggagccggtg cgtcgcatca ggcatcgagc tgcgcctcgc cagccgcccg
      121 tgtgcgttcg aggcgggcca ggctgcgacg gaggccgccg aagcccccac gcccacgacc
      181 gctgaccgac acggaccagg agctcatgcg cgccaggtcg agtaggtcgg cgggaacaca
      241 cctctcgcct cgtaggcggc gaccatcgca cggtcgatgc cgagccggaa cggcgtctcg
      301 tgctcacggc ccaccgctgc ggtggtggcg gcgcggatgc gctcccactg gcggtcgccg
      361 aaggcgaggt cggaggcctc ggccgccgcg cctcgcacga cgaactcgtc gtccacctcc
      421 tcaggaggga acgagtgcag cgcgtaccgg ccgtcgcgcc tcaggtcggc gcacttcggc
      481 gacgccgtga tgacgaacgc ccacagctcg ccgtcggcca ccacggggca gatcggatgg
      541 agccgcggcg cgcccgctcg gtcgacggtg gcgaggaacc cgaggccggg gccgaactgg
      601 gtgagcagcc gctcccccgc cctcgccaca tcgggtgcgc gacgagcgaa ctcgtcccag
      661 gaagccatgg ccgggacctt aggggcgagg ctcagccggc ggtgacgggg atgcgggtgc
      721 ggtggcgggc ggccgcggtg acaccgacca gatcacctcg gaggaagtgc ggggccgctc
      781 ccgtgacgag cacctcggcg taggtaccca cccgaagcgg cgacccgctg gcgaagtgca
      841 cgagcttgtt ctggcgcgtc cgcccggtgg tgactgacgg gtccttgcgc gatggcccct
      901 cgaccacgac ctcctcgacc cggccgatgc gcgcctcgtg ccggcgcagg gcactgcgct
      961 cgagcacggc cttgagccgg tcgaaccgct cggccacccg ggcgggatcg acgaactggt
     1021 cgaccatctc ggccgcctcc gtaccgggac ggggcgagta gatgaacgtg taggcggagt
     1081 cgtactcggc agcggcggcg agctcgagcg tgcgctcgaa gtcgtcgtcg gtctcgccgg
     1141 ggaagcccac gatcaagtcg gtggtgacgg cgaggtccgg caccgccgcc cgggccgccg
     1201 ccagcttctc caggtagcgc tccgcggtgt agccgcggtg catggcggcg aggacccggt
     1261 cgctgcccga ctgcacgggg aggtggaggt gctcgcacac ctcgggcgtc tcggccatcg
     1321 ccgcgatcgt ctcggctcgg aggtccttcg ggtgcgggct cgtgtagcgg acccggcgaa
     1381 tgccgtcgac ggcgccgacc tcgcggagca ggtcggcgaa cagcggccgg acccgcacgt
     1441 cctcgccggc ctggcgggcg gcgagcatga ggtcgcggcc gtagctgttc acgttctggc
     1501 cgagcagcgt cacctcggtg acgccggtgg cggccaggtc gcgcacctcg tcgaccagcc
     1561 ggccgaacgg gcgggagatc tcctcgcccc ggaccgccgg gacgatgcag aaggcgcagg
     1621 agttgtcgca gccgatctgg atcgtgaccc aggcggtgta gccctcgtcg cgccgggcgg
     1681 gcatggccga ggggaacagc tcgtggtcgt cggcgacggt cgcctcgagg atctcggtga
     1741 gggggccctg ctcccggctg cggtgcagca gctcggtggc ccggtgcacg ttgtgggtgc
     1801 cgaagaccac gtcgacgtag ggcgcccgct cccggatgcc ctccttgtcc ttctgggcca
     1861 ggcagcccga caccacgatc tccatgcccg gccggcggtc cttcagcgac ttgaggtgcc
     1921 cgagcgtgcc gtagagcttg ttgtcggcgt tctcccggat gcagcaggtg ttgaggacca
     1981 cgacgtcggc ctgctcgagg tcatcggtgg cctccatccc gtcggcgtcg aacagccccg
     2041 cgatccgctc ggagtcgtgc tcgttcatct ggcacccgaa ggtgcgcacg aggtagcgct
     2101 tcgggtcggg agcagcgtcg gtcatggccc gacgagtcta cggagcacct accgaggtcg
     2161 tgagatccac cgggcgcaga tccgtcgaca caagctccgg tggcgggcca gctcgaacga
     2221 cgtgtcggac tgggggtcgc agcagaccaa gcccggggcc agagggccga gcgagcacaa
     2281 atgggcttac tagtgtccga tgggtggacg tgatcgacaa ggtgagagca acgcttgcgg
     2341 ccaccaccgg tgccatcacg ctggtcgtgt accacgcgct gcgaagccgg gacatcggtt
     2401 ctggtgctgc cgttctgatc gcccttgcgg cgagcatctg cgctgcgctt gcgatcgacg
     2461 ctctgctgtt tggctacggc cttcggttcc gctctgcgag aaagatgctc ctcaagacgg
     2521 gaaagtacga gggcacgtgg ttcgtcgagc atgacgatct cgatcagcac agcctcgcga
     2581 ccatcagctc cgtcgatggg gtggtctcct accaagggat cggtgtcgag acgacttccg
     2641 ggcgcatctg cgtcgtgtgg caatccaccg acatcgcgtt cagcatcgaa agcggccggt
     2701 tccgctacgc attcgaggcg cgggtccaag gggagcccta ccgagtcggc gagggtccgg
     2761 caagccagtc acggctcctg cgcggatacg ggatactcgc cttcaacgag gaggtgaggg
     2821 gtgaacctca caccggtctg gacacctata tcgacatcga ctcagaccca tcggtcgagt
     2881 cgatgaaggc gtccgagcat tgggg
//
LOCUS       DATMFS010000180         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_52997, whole genome shotgun sequence.
ACCESSION   DATMFS010000180 DATMFS010000000
VERSION     DATMFS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52997"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2135
                     /locus_tag="VNQ33_03360"
     CDS             303..2135
                     /locus_tag="VNQ33_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ97171.1"
                     /translation="MSTTTFAALGVPDAIAAALADRGIDEPFPIQVATIPPALAGRDL
                     CGKAPTGSGKTIAFGVPMVARVTRSAPKKPHGLVLAPTRELAIQVAEELENISGDLRI
                     LAVYGGAGIEPQIKKLRAGIDVIVATPGRLIDLLDRNCCDLRSVTFAVVDEADRMADM
                     GFLPDVKRILDETRDDRQTVLFSATLDGAIDSLVKRYQKNPVVHEIEDEETGDVVHHF
                     WLAPRPDRVALTAEIVDRLGPTVVFSRTKHGSDRIARQLGNAGVKAAAIHGNRSQAQR
                     ERALEAFHRGDVQALVATDVAARGIHVDGVLGVVHFDLPHDEKDYIHRSGRTGRAGMT
                     GTVVTLIDANQRKDAVKIKKGAGVGDVEISEPDVASLPEGPQQAFRPKSRKKAQVKDL
                     APRTPANSPSRAKAKAKSKANTDRQRDWDEPKPRGARPSRPSGRNDDRSGARPSDLSR
                     GSARGSGRPGRSEREHRADHGSPRSSRPEDRSERSERQARWDGEERATKSSRENRADR
                     RNRQFTDEERARRAEANEREEARRDQQRSTDRASHRAKQSSSNGSSDRTLPGDVAGGA
                     KVRPSGASRRKAKREVLAASGEEVPRAKKRRSGKPRPPAKHRAR"
     gene            2203..2826
                     /locus_tag="VNQ33_03365"
     CDS             2203..2826
                     /locus_tag="VNQ33_03365"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ97172.1"
                     /translation="MGRTQEERRAETRGLLLSAAAELFAQKGFHATSAEAVATAADRT
                     TGALYNHFGGKEGLLVGLLEQWVEQTIVDIGAAIEGETDLDGRFGALWDGVVRRDSES
                     GDAWLLLEFELWLHAVRDPELGAVGAQRFRVMRAGLAGGLEDWSQEFGFELPAPSDDL
                     AAQVIALLLGAAFQYRLDPAAFPQPVVVSGLRRILGLPAPVTSGANR"
     gene            2823..4040
                     /locus_tag="VNQ33_03370"
     CDS             2823..4040
                     /locus_tag="VNQ33_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ97173.1"
                     /translation="MSLTLDQVDLLEDTWAKGVPYDQFALLRAEAPVFWHEHPVYTGF
                     WAVTRYDDVRAVSRDHTTFSAELGSTFLQDMTEEQMELVRMTILNMDPPKHSRFRRLV
                     SAGFKPRMIAQLVEHIEELADDIVADLEGRDEIEFVERVAAELPLRVICELLGVPVED
                     RHLVFEWSNRMVGAQDPDFRVSEADGEAAMAEIYLYCDALAEDRRQNPQDDIMTALAL
                     AEVDGDRLSKEELNMFFVTLVVAGNETTRNLISHSMVAILDQPGLKAQLVDGIDDDGL
                     WASATEEFLRWGASIHNFRRTATVDTEIAGQPIKAGDKVVIYYPAANRDPDHFTDPDV
                     FDARRTPNDHVTFGGGGTHFCLGANLARAQIKTMLRTFLRRYPNVAPAGEHKRMRSDF
                     VNGIKYLPVRLNG"
     gene            4111..5241
                     /locus_tag="VNQ33_03375"
     CDS             4111..5241
                     /locus_tag="VNQ33_03375"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HWJ97174.1"
                     /translation="MQTDVAEKLGIEFPIFAFTHCRDVVVAVSKAGGLGVLGAVGFTP
                     EQLEVELNWIDEHIGDKPYGVDIVIPGKYEGMGATDADKLEEDLKAMIPQEHRDFVQK
                     LLAEHNVPDLPEEEKMRELIGFGTAIAGPQAEIALAHDKVVLLANALGTPPPDVIEDV
                     HSKGKFIAALCGSAAQAARHKAAGVDIVIAQGTEGGGHTGEIGSIVLWPEVLDVLGDT
                     PMLAAGGIGSGRQMAAALAMGAQGVWTGSLWLTVAEADVPPAQMQSYFDATSKDTLRS
                     RSWTGKPCRMLRNDWTDAWEDPANPAPLGMPMQFMVTSNAVQRGHKYPEQAKDVQFNP
                     VGQIVGRFEKMRPSRDVIFEMVEECIEATERMSNLMNQSVEA"
     gene            5246..>6311
                     /locus_tag="VNQ33_03380"
     CDS             5246..>6311
                     /locus_tag="VNQ33_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ97175.1"
                     /translation="MSIDRCPVAHGTDFTDPDLVQERVPLPEFAYLRQNEPMFWNPQT
                     RENSSFDDGGFWLATRHEDVKAISCARDGWSSEENTAIVKFEGSIGKDERDIQKQMML
                     NMDDPHHAKVRGIVSRGVFTPRGVSKIEDALRARANAICTEAKAKGTGNFVEDVACEL
                     PLQALADLMGFPQDDRKQIFDWSNQMMAYDDPDFEVDPAVAAAEILGYAMTIGETRKA
                     CPADDIITKLVSASTESDTLTTEEFAWFVLMLAVAGNETTRNAISHGMHAFMQFPEQW
                     EIYKRDRPETAADEIVRWATPVVMFQRTALEDAEVAGQHVKAGQRVGLVYSSANFDET
                     VFEDPHSFNILRDPNPHVGFG"
BASE COUNT         1064 a         2343 c         2079 g          825 t
ORIGIN      
        1 gctggaacgc cgcggtggac gagtggttcg ccgccaaccc ggagtgacct ctgaccacct
       61 cgcaggaccg cggcccgccg agctcggtcc ggtaggttgg gctggtcgat cagtgcgggc
      121 tctggccttc gcgatcggac agcgatctca gagggggcgc cgagaggcga acccaagacc
      181 cacgttcggg tccgtcggct ccggctgaca ggagcgggag atggctgtcg acctcggctc
      241 cggccgacgc cgttcctgcc acacccgatc cgccctccgt ggcgtcaagg agatctgccc
      301 tcatgtccac caccacgttc gccgccctcg gcgtgcccga tgcgatcgcg gccgcgctcg
      361 ccgaccgcgg catcgacgag cccttcccca tccaggtcgc caccatcccg ccggcgctgg
      421 ccgggcgcga cctctgcggc aaggcgccca ccggctccgg caagacgatc gccttcgggg
      481 tgccgatggt cgcccgcgtc acccgctccg ccccgaagaa gccgcacggc ctggtgctgg
      541 cgccgactcg cgagctggcc atccaggtgg ccgaggagct cgagaacatc tcgggtgacc
      601 tccgcatcct cgccgtctac ggcggtgccg ggatcgagcc ccagatcaag aagctgcgcg
      661 ccggcatcga cgtcatcgtc gccacgcccg gccgcctgat cgacctgctg gaccgcaact
      721 gctgcgatct ccgctcggtg acgttcgccg tcgtcgacga ggccgaccgc atggccgaca
      781 tggggttcct ccccgacgtg aagcggatcc tcgacgagac gcgcgacgac cgccagaccg
      841 tcttgttctc cgccaccctc gacggagcca tcgactccct cgtgaagcgg taccagaaga
      901 acccggtggt ccacgagatc gaagacgagg agacgggcga cgtcgtccac cacttctggc
      961 tggccccccg ccccgaccgc gtggccctca ccgccgagat cgtcgaccgg ctcggcccga
     1021 ccgtcgtctt cagccgcacc aagcacggtt cggaccgcat cgcccgccag ctcggcaacg
     1081 ccggcgtgaa ggcggcggcc atccacggca accgcagcca ggcgcagcgc gagcgggcgc
     1141 tcgaggcgtt ccaccgcggc gacgtgcaag cgctcgtggc caccgacgtc gccgcccgcg
     1201 gcatccacgt cgacggcgtc ctcggcgtgg tccacttcga cctcccgcac gacgagaagg
     1261 actacatcca ccgctcgggc cgcaccggcc gcgccgggat gaccggcacg gtcgtcaccc
     1321 tcatcgacgc caaccagcgc aaggacgcgg tgaagatcaa gaagggcgcc ggtgtcggcg
     1381 acgtcgagat cagcgagccg gacgtggcgt cgctgcccga ggggccccag caggcgttcc
     1441 gcccgaagtc ccgcaagaag gcccaggtca aagacctcgc cccccgcacg ccggccaaca
     1501 gcccgagccg ggccaaggcc aaggccaaga gcaaggcgaa caccgaccgc cagcgggatt
     1561 gggacgagcc caagccccgc ggcgcgcgcc cgagccggcc gagcggtcgc aacgacgacc
     1621 gcagcggcgc ccgcccctcc gacctcagcc ggggctcggc tcgtgggtcg ggtcggcccg
     1681 gacgcagcga gcgcgagcac cgcgccgacc acggttcgcc ccgctcctcg cgtccggagg
     1741 accgctccga gcgctccgag cgccaggctc gctgggacgg cgaggagcgt gccaccaagt
     1801 cgagccgcga gaaccgggcc gaccgccgca accgccagtt caccgacgag gagcgcgccc
     1861 gccgggctga ggccaacgag cgcgaagagg cccgccgcga ccagcagcgc tcgaccgacc
     1921 gcgccagcca ccgtgccaag cagtcgagct cgaacggctc ctccgaccgc accctccccg
     1981 gcgacgtcgc cggcggcgcc aaggtccgcc ccagcggcgc cagccgccgc aaggccaagc
     2041 gcgaggtgct cgccgccagc ggcgaagagg tcccccgcgc caagaagcgc cgcagcggca
     2101 agccccgccc cccggccaag caccgcgctc gctgacgccg tgtgacctgt tgcatacatc
     2161 ggatgctcga catttgtagg gtacccgatg taacttgacg gcatggggag aacgcaggag
     2221 gaacggcgcg ccgagacgcg cgggctgctg ctgagcgcgg cggcggagct gttcgcccag
     2281 aagggcttcc acgcgacctc cgcggaggcg gtggccacgg cggctgatcg cacgacgggt
     2341 gccctctaca accacttcgg cggcaaggag ggcctgctcg tcgggctgct ggagcagtgg
     2401 gtcgagcaga cgatcgtcga catcggcgcg gcgatcgagg gcgagaccga cctcgacggg
     2461 cggttcggcg cgctgtggga cggggtcgtc cgccgcgaca gcgagagcgg cgacgcctgg
     2521 ctcctgctcg agttcgagct gtggctgcac gccgtgcgcg acccggagct gggggccgtc
     2581 ggcgcccagc gcttccgggt catgcgcgcc ggtctggcgg gcggtctcga ggactggtcg
     2641 caggagttcg gcttcgagct gcccgcgccg tccgacgacc tggccgccca ggtgatcgcc
     2701 ctgctgctcg gcgccgcgtt ccagtaccgc ctcgaccctg ctgcgttccc gcagccggtc
     2761 gtggtgtcgg gcctgcgccg gatcctcggc cttcccgcac ccgtcacgtc cggagcgaac
     2821 cgatgagcct caccctcgac caggtcgacc tcctcgaaga cacctgggcg aagggggtgc
     2881 cctacgacca gttcgcgctg ctgcgcgccg aggcgcccgt gttctggcac gagcacccgg
     2941 tgtacacggg cttttgggcg gtcacccgct acgacgacgt ccgtgcggtc agccgcgacc
     3001 acacgacctt cagcgccgag ctgggctcga cgttcctcca ggacatgacg gaggagcaga
     3061 tggagctggt gcggatgacg atcctcaaca tggatccgcc gaagcactcg cgcttccgcc
     3121 ggctcgtcag cgccgggttc aagccccgca tgatcgccca gctggtagag cacatcgagg
     3181 agctggccga cgacatcgtc gccgacctcg agggcaggga tgagatcgag ttcgtcgagc
     3241 gcgtggccgc cgagcttccc ctgcgggtga tctgcgagct gctcggcgtg ccggtcgagg
     3301 accgccacct cgtgttcgag tggagcaacc gcatggtggg cgcccaggac cccgacttcc
     3361 gggtgtccga ggcggacggc gaggcggcga tggccgagat ctacctgtac tgcgacgcgc
     3421 tggccgagga ccgccggcag aacccgcagg acgacatcat gacggcgctc gcgctggccg
     3481 aggtcgacgg cgaccggttg agcaaggaag agctgaacat gttcttcgtc accctcgtgg
     3541 tggcgggcaa cgagacgacc cggaacctga tctcgcactc gatggtcgcg atcctcgacc
     3601 agcccggcct caaagcgcag ctggtcgacg gcatcgacga cgacgggctc tgggcgtcgg
     3661 ccaccgagga gttcctccgc tggggtgcgt cgatccacaa cttccggcgc accgccacgg
     3721 tcgacaccga gatcgccggc cagccgatca aggccggcga caaggtcgtg atctactacc
     3781 cggctgcgaa ccgcgacccg gaccacttca ccgatcccga cgtgttcgac gcccgccgca
     3841 cgcccaacga ccacgtcacc ttcggcgggg gcggcaccca cttctgcctc ggcgccaacc
     3901 tcgcccgggc ccagatcaag acgatgctgc gcacgttcct ccgccgctac ccgaacgtcg
     3961 cgccggccgg cgagcacaag cgcatgcgct ccgacttcgt caacggcatc aagtacctcc
     4021 cggtccgcct gaacggctga cgccccggac cacccaccgt tccaaccgac cgatcccacc
     4081 gtttccacca tctcgaaagc agacccctcc atgcagaccg acgtagccga gaagctcggc
     4141 atcgagttcc cgatcttcgc cttcacccac tgtcgcgacg tcgtcgtggc ggtcagcaag
     4201 gccggcggcc tcggcgtctt gggcgccgtc ggcttcaccc ccgagcagct cgaggtcgag
     4261 ctcaactgga tcgacgagca catcggcgac aagccctacg gcgtcgacat cgtcatcccc
     4321 ggcaagtacg agggcatggg cgccaccgat gcggacaagc tcgaggaaga cctcaaggcg
     4381 atgatccccc aggagcaccg cgacttcgtg cagaagctcc tggccgagca caacgtgccg
     4441 gatctccccg aagaggagaa gatgcgcgag ctgatcggct tcggcaccgc catcgccggc
     4501 ccccaggccg agatcgccct cgcccacgac aaggtcgtgc tcctcgccaa cgcgctgggc
     4561 acgccgccgc cggacgtgat cgaagacgtc cactcgaagg gcaagttcat cgccgccctc
     4621 tgcggctcgg ccgcccaggc ggcccgccac aaggcagcgg gtgtcgacat cgtcatcgcc
     4681 cagggcaccg aaggcggcgg ccacaccggc gagatcggct cgatcgtgct gtggcccgag
     4741 gtgctcgacg tgctcggcga cacgcccatg ctggccgcgg gcggcatcgg cagcggccgc
     4801 cagatggccg ctgctctcgc catgggtgcg cagggcgtgt ggaccggctc cctgtggctc
     4861 accgtcgccg aggccgatgt gccgccggcg cagatgcaga gctacttcga cgccaccagc
     4921 aaggacaccc tgcgcagccg ctcgtggacc ggcaagccct gccgcatgct ccgcaacgac
     4981 tggacggacg catgggagga cccggcgaac ccggcgccgc tcggcatgcc gatgcagttc
     5041 atggtcacct cgaacgccgt ccagcgcggc cacaagtacc ccgagcaggc caaagacgtg
     5101 cagttcaacc cggtcggcca gatcgtcggc cgcttcgaga agatgcgccc gtcgcgggat
     5161 gtgatcttcg agatggtgga ggagtgcatc gaagccaccg agcgcatgag caacctgatg
     5221 aaccagagcg tggaggcctg atcagatgag catcgaccgc tgtcccgtcg cccacgggac
     5281 cgacttcacc gaccccgacc tcgtgcagga gcgcgtgccg ctgcccgagt tcgcctacct
     5341 ccgccagaac gagccgatgt tctggaaccc gcagacccgt gagaactcga gcttcgacga
     5401 cggcggcttc tggctggcca cccgccacga ggacgtcaag gccatctcct gcgcccgcga
     5461 cggctggtcg agcgaggaga acacggccat cgtcaagttc gagggctcga tcggcaagga
     5521 cgagcgcgac atccagaagc agatgatgct gaacatggac gacccgcacc acgccaaggt
     5581 gcgcggcatc gtgtcccgcg gcgtgttcac cccccggggc gtgtcgaaga tcgaagacgc
     5641 cctccgtgcc cgcgccaacg ccatctgcac cgaggccaag gccaagggca cgggcaactt
     5701 cgtcgaggac gtcgcctgcg agctcccgct ccaggcgctg gccgacctga tgggcttccc
     5761 gcaggacgac cgcaagcaga tcttcgactg gtcgaaccag atgatggcct acgacgaccc
     5821 ggacttcgag gtcgatccgg ccgtcgccgc cgccgagatc ctcggctacg ccatgaccat
     5881 cggagagacc cgcaaggcgt gcccggccga cgacatcatc accaagctgg tgtcggccag
     5941 caccgagtcc gacaccctca cgaccgagga gttcgcctgg ttcgtgctga tgctggcggt
     6001 ggccggcaac gagaccaccc gcaacgccat cagccacggc atgcacgcct tcatgcagtt
     6061 cccggagcag tgggagatct acaagcgcga ccgccccgag accgcggccg acgagatcgt
     6121 ccgctgggcc acgcccgtcg tcatgttcca gcgcacggcg ctcgaagacg ccgaggtcgc
     6181 cggtcagcac gtgaaggccg gccagcgcgt cggactcgtc tactcgtcgg ccaacttcga
     6241 cgagacggtg ttcgaggacc cgcactcgtt caacatcttg cgcgacccca acccgcacgt
     6301 cggcttcggc g
//
LOCUS       DATMFS010000181         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_286377, whole genome shotgun sequence.
ACCESSION   DATMFS010000181 DATMFS010000000
VERSION     DATMFS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286377"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VNQ33_03385"
     CDS             <1..614
                     /locus_tag="VNQ33_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWJ97176.1"
                     /translation="RWANGDPHGARSTLLTEAELAHHDHHNGSAAWLWHDASRLGARG
                     LTEQIRSVADTGDSPVVAARAAHVSALEAGDADGLVAAADAFEALGAILLAAEAASDA
                     SSELRRSGDQRRGAALANRSKQLMERCPGVRTPSLAPADVAVPLSAREREVASLAGSG
                     LTSQEIADRLFLSIRTVDNHLQKAYAKLGVNRRDQLAQALGEA"
     gene            complement(924..1727)
                     /locus_tag="VNQ33_03390"
     CDS             complement(924..1727)
                     /locus_tag="VNQ33_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97177.1"
                     /translation="MSRTVVVTGSASGMGAATARRLVATGHRVIGVDLHDATVIADLG
                     TRAGRASAIAEVAGLVGGPSGSLDGVVTWAGLAGLTDRPGSLLASVNYFGTVELLDGL
                     RPLLARGDRPAAVAISSNATVIQPNVPDAVTEACLSGDEDEARTIADEVGSLNAYPAT
                     KTAVARWVRRHAPGAAWAGAGITLNAVAPGAVETPLLQESRQDRTVGEFVDALPIPAG
                     RKGTAEELAGFVEFLLGPDARWFCGSFLLVDGGTDASLRPDDTPANWVP"
     gene            2008..>2796
                     /locus_tag="VNQ33_03395"
     CDS             2008..>2796
                     /locus_tag="VNQ33_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97178.1"
                     /translation="MKKLLGLLAVLALTVAAASCGDRGSDSSGGTDSGSTTAPASDGA
                     GGKGDWGDLKGVCGPKEKGGEVPSGDDSQGVSEDSITLGTVADPGFSARPGLNQEIFD
                     AGDAFVEWCNAAGGINGKQLKLNKHDAKLTDYKPALDDACKTDFAMVGGGAVQDNLWE
                     TTGKACDLIDVAGFAVTPEKAGLAGQQPSETRTVQPVPNPGDRYIVAGLKLVDDKFPG
                     ALDKTGFLTADFQTLAAQFTKESQGFEAEGADIVYKGTYGINGEA"
BASE COUNT          414 a         1030 c         1017 g          335 t
ORIGIN      
        1 cgcgctgggc caacggtgac ccgcacggtg cccgttccac gctcctcacc gaggccgagc
       61 tcgcccatca cgatcaccac aacgggtcgg ccgcgtggct gtggcacgac gcatcccgcc
      121 tcggcgcccg cggcctgacc gagcagatcc ggtcggtcgc cgacacgggc gacagcccgg
      181 tggtcgccgc ccgcgccgcc cacgtcagcg cgctcgaagc cggcgatgcc gacgggctcg
      241 tcgctgccgc ggacgcgttc gaggcgctcg gcgccatcct cctcgccgcc gaggccgcgt
      301 cggatgcgtc gagcgaactg cgccgcagcg gcgatcagcg gcgcggcgca gcgctggcca
      361 accggtccaa gcagctgatg gagcgctgcc ccggagtccg cacgccctcg ctcgcccccg
      421 cggatgtggc cgtcccgctg agcgcccgcg agcgggaggt ggcgtcgctc gccgggagcg
      481 ggctcacgag ccaggagatc gccgaccgcc tcttcttgtc gatccgcacc gtcgacaacc
      541 acctccagaa ggcgtacgcc aagctcggcg tgaaccgccg cgaccagctg gcccaggcac
      601 tcggcgaggc ctgagccagc cgccccggag aggaaccggg agatgagtag tcactgctca
      661 tgacccccga ggccgcggtc cgtaccgtca cgtcggtgcg ccgggccggc cggaccaccc
      721 tcttcaccgg ccggatcacg gtcctgccgg ccgccgctcc agacgggagg gcggcggccg
      781 gccgactcgt gcggccgtcg ccccagcggg aggggcggcg gccgtccgaa cacccgtctc
      841 cggccggttg ccctgcccgg accccgacgg tgagcgctcc cgccaggggg cggtggccga
      901 cggagctcgc acgggtgcgg cgctcagggg acccagttgg cgggggtgtc gtcggggcgg
      961 aggctggcgt cggtaccgcc gtcgaccaag aggaacgagc cgcagaacca gcgggcgtcg
     1021 gggccgagga ggaactcgac gaaccccgcc agttcctcag ccgtgccctt gcggcccgcg
     1081 gggatcggga gggcgtcgac gaactcgccg acggtccggt cctgccggct ctcctgcagc
     1141 agcggcgtct cgacggcgcc cggcgccacc gcgttgagcg tgatgccggc cccggcccag
     1201 gccgcacccg gggcgtggcg ccgcacccag cgggcgaccg ccgtcttggt ggcgggatag
     1261 gcgttgagcg agccgacctc gtcggcgatg gtccgggcct cgtcctcgtc tcccgagagg
     1321 cacgcctcgg tgacggcatc ggggacgttg ggctggatca cggtggcgtt ggagctgatg
     1381 gccaccgccg cgggccggtc gccccgggcc agcaggggac ggagcccatc gagcagctcg
     1441 acggtcccga agtagttgac cgaggcgagc agggagcccg ggcggtcggt gagcccggcc
     1501 agccccgccc acgtgaccac gccgtcgagc gagcccgacg gcccgcccac cagcccggcg
     1561 acctcggcga tggccgacgc ccgtccggcc cgggtgccca ggtcggcgat cacggtggcg
     1621 tcgtggagat cgacaccgat gacccggtgg cccgttgcga cgaggcggcg ggcggtggcg
     1681 gcgcccatgc ccgaggctga accggtgacg acgacggtgc ggctcatgcg acgcgctccg
     1741 ctccgggcgc cgcggcgcgg aggggccgat cggccgacgg caggcgctcg tcgccgtgga
     1801 tcgctctggt ggccggttcc aggtcatccg acatgaacag agcatggccg aagatttgac
     1861 ggtccgtcag aaccgcccag caccggccac ccgatcgggg taggttccgg tcgtcgccgt
     1921 ggagacggcg tcgtgctgtc aaggggtgca gtggtgaacg ggctccggtc cctcctcgct
     1981 gcatggacac gaacagatgg ggaaaacatg aagaagctcc tcggcctgtt ggccgtgctc
     2041 gcgctcaccg tcgccgccgc cagctgcgga gaccgcggct cggactcctc gggaggcacc
     2101 gacagcggct cgaccaccgc accggcgtcc gacggcgccg gcggcaaggg cgactggggc
     2161 gacctgaagg gcgtctgcgg cccgaaggag aagggcggcg aggtgccctc cggcgacgac
     2221 tcgcagggcg tgagcgaaga cagcatcacg ctcggcaccg tggccgaccc cggcttttcg
     2281 gcccgccctg gtctcaacca ggagatcttc gacgccggcg atgcgttcgt cgagtggtgc
     2341 aacgccgcgg gcggcatcaa cggcaagcag ctgaagctca acaagcacga cgccaagctc
     2401 acggactaca agccggcgct cgacgacgcc tgcaagaccg acttcgccat ggtcggcggc
     2461 ggcgccgtgc aggacaacct ctgggagacc accggcaagg cctgcgacct catcgacgtg
     2521 gctggcttcg ccgtcacgcc ggagaaggcc gggctcgccg gccagcagcc ttcggagacc
     2581 cgcaccgtcc aacccgtgcc caaccccggt gaccgctaca tcgtcgccgg cctcaagctg
     2641 gtcgacgaca agttccccgg cgccctggac aagaccggct tcctcacggc cgacttccag
     2701 accctggcgg cgcagttcac gaaggagtcg caaggcttcg aggccgaggg cgccgacatc
     2761 gtctacaagg gcacctacgg catcaacggc gaggcc
//
LOCUS       DATMFS010000182         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_30697, whole genome shotgun sequence.
ACCESSION   DATMFS010000182 DATMFS010000000
VERSION     DATMFS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30697"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VNQ33_03400"
     CDS             complement(<1..1912)
                     /locus_tag="VNQ33_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97179.1"
                     /translation="MKVLQVREAAGDRPAIELHGQVTVVRGLDPLRRSWLIDVLGRLA
                     SGQDPDAAGELVAHGVVFPLDRTSLALLDLERPVPSVVRSEDLPGHDPRVAQAVSDRD
                     RALIRRRELTQQITEQREALGAAVAERTDATEELEALRRGDGAAREAQAAADAARARL
                     DAELASASAERARNREALNSAVLARGVASEARVASAERLERARERRRTAMKKATEAAA
                     ALEQARPLVADDPTDLLVARRVELASAEAAAGEADPDQDSSPVSRMLADLERRRVELV
                     RLQEAVGDPGAGAVRGSLDQLLGAGTEAAPVVAALALADTWRDLHQQLSALDSGVSGE
                     EYAAEQRVAEARQAVIEAEVEYNQPVLTPEQITKVEAAHALVLEAQDRTEGRFGGSRL
                     RKKLEDARAEERRVLERLGFSTYADYMMSSSSRGVGPANRSTLETARARLKLAEEGLA
                     ALPGAADRARRRTELLQRRDAVAPRVAALLGHEPTGPESEDELRSLREPAAPSDSAMA
                     ELARQLTAVGVAVVGPPYDRDDLVLLARFYLSEQETADERRSDIDHALDALDHAILAV
                     RDARDRGQHDLPPLDPLPELAQPIAEPAADGDAAAQAVTLRQARWADVEAARSAVAEA
                     EAAVAQHRQASAA"
BASE COUNT          239 a          742 c          777 g          261 t
ORIGIN      
        1 gcgcggcgct cgcctggcgg tgctgggcga cggccgcttc ggcctcggcc accgcggacc
       61 gagccgcctc gacgtcggcc cagcgggcct ggcggagggt gacggcctgg gcagcggcat
      121 cgccatccgc ggcgggctcg gcgatgggct gggcgagctc cggcagcggg tcgagcgggg
      181 gaaggtcgtg ctggccccgg tcacgagcgt cgcggaccgc gaggatggcg tggtcgagcg
      241 cgtcgagggc gtggtcgatg tcggaccgac gctcgtcggc ggtctcctgc tcggacaggt
      301 agaagcgcgc cagcaagacg aggtcgtcgc ggtcgtacgg gggcccgacg accgccacgc
      361 ccaccgcggt gagctggcgg gccagctcgg ccatggccga gtcgctgggg gcggccggct
      421 cgcgcaggga gcggagctcg tcttcggact ccggaccggt gggctcgtgc ccgaggagcg
      481 ccgccactcg gggcgccacg gcatcgcgcc gctgcagcag ctcggtgcgg cggcgggcgc
      541 ggtcagcagc acccgggagc gcggccaggc cctcctcggc cagcttgagc cgggcccggg
      601 cggtctcgag ggtcgagcgg tttgccgggc cgacgccgcg gctcgacgag ctcatcatgt
      661 agtcggcgta ggtggagaag ccgagccgtt cgaggacccg gcgctcctcg gcccgggcgt
      721 cctcgagctt cttgcggagt cggctgcccc cgaagcgccc ctccgtacgg tcctgggctt
      781 cgaggacgag cgcgtgggcg gcctcgacct tggtgatctg ctcgggcgtg aggaccggct
      841 ggttgtactc gacctcggcc tcgatgaccg cttgtcgagc ctcggccacc cgctgctcgg
      901 cggcgtactc ctcacccgac acaccggaat ccagagcgct cagctgctgg tgcaggtcgc
      961 gccaggtgtc ggccagcgcc aaggcggcga ccaccggggc ggcctcggtc cccgcgccga
     1021 ggagctggtc gagcgagccg cggaccgcac cggccccggg gtcgccgacg gcctcctgga
     1081 ggcgcaccag ctcgacccga cgccgttcga gatcggccag catccggctg acaggcgagc
     1141 tgtcctggtc tgggtcggcc tcgccagcgg cagcctcggc gctggcgagc tcgacgcggc
     1201 gggcgaccag caggtcggtc gggtcgtcgg ccacgagcgg acgggcctgc tcgagcgcgg
     1261 ccgctgcctc cgtcgccttc ttcatcgccg tccgccggcg ctcgcgagcc cgctcgagcc
     1321 gctcggctga cgcgacccgg gcctcgctgg ccacgccacg ggccaacacg gcgctgttga
     1381 gcgcctcgcg gttgcgggcg cgctccgccg atgctgacgc gagctcggca tcgaggcggg
     1441 cccgcgccgc atcggccgca gcctgcgcct cgcgtgctgc gccgtcgccg cggcgcaggg
     1501 cctccagctc ctcggtggcg tccgtccgct cggccacggc cgcgcccagc gcctcgcgct
     1561 gctcggtgat ctgctgggtg agctcccggc ggcggatcag cgcccggtcg cggtcggaga
     1621 cggcctgcgc cacgcgcgga tcgtgtccgg gaaggtcctc ggagcggacc accgacggga
     1681 ccggtcgctc gaggtccaag agcgcgagcg aggtgcggtc gagcgggaag acgacgccgt
     1741 gggccaccag ctcaccagcg gcatccgggt cctgccccga agcgagacgc cccaggacat
     1801 cgatgagcca ggagcgccgc agtggatcga ggccccgcac cacggtcacc tggccgtgca
     1861 gctcgatcgc ggggcggtca ccggctgcct cgcgcacctg gagcaccttc actgcacacc
     1921 ctcccgttcc cacggccgat cgatcgtgag gctgcgagga cggtacctga tcggcccgtc
     1981 agcgcggtag ctccccccgc aggtcaggag accgggatg
//
LOCUS       DATMFS010000183         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53014, whole genome shotgun sequence.
ACCESSION   DATMFS010000183 DATMFS010000000
VERSION     DATMFS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53014"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VNQ33_03405"
     CDS             <1..474
                     /locus_tag="VNQ33_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWJ97180.1"
                     /translation="LAEADLPVNLAVSLHLANDAERDVLVPINKRYPLDVLAGACADY
                     VDATHRRLSFEWALIDGVNDTDRHAHELADYARPLRAHVNVIPLNPTPGYAVRGTPPE
                     GVRAFAALLDRLGINATIRRTRGTDIDAACGQLRATSGSAEPTPVAAPARRSTSG"
     gene            complement(476..>2265)
                     /locus_tag="VNQ33_03410"
     CDS             complement(476..>2265)
                     /locus_tag="VNQ33_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWJ97181.1"
                     /translation="APSPMTLSRYDEWDGTYATTSNRSANYGLYAFRNPDTPYKRAFW
                     HPGIGIWQYDSAGVGAPYTAIERMDVRTTSADVARGMAARYCSPSASVIGHGPPYSGL
                     ERRYAAWTPWGYPCTLCEQEFQAMTSGTDFANITLVDGISVTGGAKQRSCTLTGVTGV
                     LPCWYVDPSVGTIEGATGWAQIAPLDGGSPTSTPTPLAAPFYVIDRGATEERHWLRAD
                     SGYSIDIMGTRTIGTNERPRSNQAGSGVTWRSGSGLCDLTTGRGACLPPAPPGLSLTV
                     ANVSGTFRPISLDAQGDGKGDILWYAPGAAADYLWSGQGSGRFSDAKVSIGGRYSDVL
                     PVDIDADGDDDILWYDRASGGSYLWAADGRGGYRSVTLTRPKGLRPFIVDTDGNGREE
                     IFWYAPGAAADSLWAWNGLAFTSRTQSVSGSYVPVAGDFDGNGRDDVLWYAAGSTADH
                     LWLSTGLGTHRDVAVSVAGSYTPLVGDFDGDGKDDVFWYAPGSVPDHVWFGGPGGAFA
                     SRSTPVSGTYRPLVVDLEHDGRDDVVWYAPGPPSDAWWRWGADRSATAASVIADGTHQ
                     PIVGAFGSSGGDGIFWYAPGPTPDGTWWR"
BASE COUNT          311 a          842 c          814 g          298 t
ORIGIN      
        1 ctggctgagg cggacctccc ggtcaacctc gccgtctccc tgcacctggc gaacgacgcc
       61 gagcgcgacg tcctcgtgcc catcaacaag cggtacccgc tcgacgtcct cgccggtgcg
      121 tgcgccgact acgtcgacgc cacccaccgg cgcctgtcgt tcgagtgggc gctgatcgac
      181 ggggtcaacg acaccgaccg ccacgcccac gagctggccg actacgcccg gccgctccgc
      241 gcccacgtca acgtgatccc gctcaacccc acgccgggct acgcggtgcg gggcacgccg
      301 cccgaagggg tgcgggcgtt cgccgccctg ctcgaccgcc tggggatcaa cgccacgatc
      361 cgccgcaccc gcggcaccga catcgacgcc gcgtgcgggc agctgcgagc caccagcggc
      421 agcgccgagc ccacgcccgt ggccgctccc gcccgccgct cgacctccgg gtagctcagc
      481 gccaccaggt gccgtcgggg gtcggtccgg gggcgtacca gaagatcccg tcgccgccgc
      541 tgctcccgaa cgcgcccacg atcggctggt gggtgccgtc ggcgatgacc gaggctgcgg
      601 tcgccgagcg gtccgccccc caccgccacc aggcatcgga cggcgggccg ggcgcgtacc
      661 agacgacgtc gtcgcggccg tcgtgctcga ggtcgaccac gagcggccgg taggtccccg
      721 agaccggcgt gctccgtgag gcgaacgcgc cgcccggacc gccgaaccac acgtggtccg
      781 gtaccgatcc gggggcgtac cagaacacgt cgtccttgcc gtctccgtcg aagtcgccca
      841 ccagcggggt gtacgagccg gcgaccgaga ccgccacgtc gcggtgcgtg cccagacccg
      901 tggagagcca gaggtgatcg gcggtcgagc cggcggcgta ccagaggacg tcgtctctgc
      961 cgttgccgtc gaagtcgccg gccaccggca cgtagctccc gctgacggac tgcgtccggc
     1021 tggtgaacgc caggccgttc cacgcccaga gcgagtcggc ggccgctccc ggggcgtacc
     1081 agaagatctc ctcgcgcccg ttgccatcgg tgtcgacgat gaacgggcgc aggcccttcg
     1141 gccgcgtcag cgtcaccgac cggtagccgc cccgtccgtc ggccgcccag aggtacgagc
     1201 cgcccgaggc ccggtcgtac cagaggatgt cgtcgtcgcc gtcggcgtcg atgtcgaccg
     1261 gcagcacgtc gctgtagcgc cctccgatgc tgaccttggc atcggagaag cgccccgagc
     1321 cctggcccga ccagaggtag tcggcggcgg cgcccggcgc gtaccagagg atgtcgccct
     1381 tgccgtcgcc ctgggcatcg agcgagatgg gccggaaggt gccgctgacg ttcgccaccg
     1441 tgaggctgag ccccggcggc gccgggggca ggcacgcgcc gcgtccggtc gtgaggtcgc
     1501 agagccccga gcccgaacgc caggtcacgc ccgacccggc ctggttggag cgggggcgct
     1561 cgttcgtccc gatcgtgcgg gtccccatga tgtcgatcga gtacccggaa tcggcccgga
     1621 gccagtgccg ctcctcggtg gctccccggt cgatcacgta gaacggtgcg gcgagcggcg
     1681 tgggggtgct ggtcgggctg ccgccgtcga gcggagcgat ctgcgcccag cccgtggccc
     1741 cctcgatggt gccgaccgag ggatcgacgt accagcacgg caggacgccg gtgacgcccg
     1801 tgagcgtgca cgaccgctgc ttcgctccac ccgtcaccga gatgccgtcg acgagggtga
     1861 tgttggcgaa gtcggtcccc gaggtcatgg cctggaactc ctgctcgcac aacgtgcacg
     1921 ggtagcccca cggggtccag gccgcgtacc gccgctcgag ccccgagtag ggcgggccgt
     1981 ggccgatgac cgaggcggac gggctgcagt agcgcgccgc catgccacgg gccacgtcgg
     2041 ccgacgtggt gcggacgtcc atgcgctcga tggccgtgta gggcgcgccg acccccgccg
     2101 agtcgtactg ccagatgccg atccccgggt gccagaaggc ccgcttgtag ggggtgtcgg
     2161 ggttccggaa ggcgtagagc ccgtagttcg cggaccggtt ggaggtggtg gcgtacgtgc
     2221 cgtcccactc gtcgtagcgc gacagggtca tcggcgacgg cgccg
//
LOCUS       DATMFS010000184         3123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_7717, whole genome shotgun sequence.
ACCESSION   DATMFS010000184 DATMFS010000000
VERSION     DATMFS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7717"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1419
                     /locus_tag="VNQ33_03415"
     CDS             85..1419
                     /locus_tag="VNQ33_03415"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWJ97182.1"
                     /translation="MPATVVVGTQWGDEGKGKFTDLVAKEMQLVVRYQGGHNAGHTLV
                     VDGESFALQLCPSGILYDHITPVIGNGVVVDPRVLIAEMDMLTSKGVDPTRLKVSGNA
                     HLILPYHQEIDRLTERRLGSRKLGTTKRGIGPAYADKSSRVGLRVQDLLDPKIFRAKL
                     EVLAKEKNAILAKVFNQLPLDPEEIADEYLDVCRPRIEPHIADTVSLVHEALERQEHV
                     LFEGAQATFLDLDHGTYPFVTSSNPVAGGVCTGAGVGPRYIDRVIGVAKAYVTRVGTG
                     PFPTELAISGEAVGGKDRELADEEEIRLGDHFVDVGHEYGTNSGRRRRPGWFDAVMLR
                     QAVRLNSLSELAITKLDILDTLDTVKVCVAYDVRGERVTHLPYHQSDLHAATPIYEEL
                     PGWKTDLSAITETHELPPAAEAYLAFLEEQVGVPVTLVGVGPGREQFVHRNA"
     gene            1513..2748
                     /gene="purD"
                     /locus_tag="VNQ33_03420"
     CDS             1513..2748
                     /gene="purD"
                     /locus_tag="VNQ33_03420"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWJ97183.1"
                     /translation="MKVVVVGSGGREHALAVVLGRTAEVVVTPGNPLIPGSVGTDVED
                     LVADGSVDLVVVGPEQPLVAGLADRLRAGGVRVFGPGAEGAKLEGSKAYMKMIAEEAG
                     LPTARWEAFTDADDAVDYLNAMKGPYVVKTDGLAAGKGVLVTDSLDDAVSDVRAKLGG
                     DTFGESGRRVVIEEALTGPEVSVFAVCDGTRAVCLPAAQDFKRVGDGDAGLNTGGMGA
                     WSPLPFLPDGFEEQVARDFIAPTLAELQQRGIDYRGVLYAGFILTDEGPKLLEYNVRF
                     GDPDSQVVLLRMTSDLAGLLAAAADGDLAKAPEPTFSDDAAVLVVAASEGYPVVPRTG
                     DAVEGLDAAASVVGVTVLGAGVGRRLDGTVTTAGGRVLNVVGIGATTDDARSKAYGAL
                     GRITWPGEHHRTDIAASPA"
     gene            2791..>3123
                     /locus_tag="VNQ33_03425"
     CDS             2791..>3123
                     /locus_tag="VNQ33_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWJ97184.1"
                     /translation="MWRLRGILAISGVVAAVVPLAAAGAAGADPVPGQVVINEIHYHP
                     VVDEQEFLELHNPGDDPVDLSGACFTAGIGGCFAAGTTLPAGGYVVAAQSLSAYATAF
                     PSAPAPAVQ"
BASE COUNT          442 a         1108 c         1125 g          448 t
ORIGIN      
        1 gtggtgggga acgccttcgg ggtgggggat cgccaggcag tagcgttgcc cccggtctga
       61 atcgccgtcg tgaggagcag ggccgtgcca gcgaccgtcg tggtcggtac gcagtggggt
      121 gacgagggga aggggaagtt caccgacctc gtcgccaagg agatgcagct cgtcgtccgc
      181 taccagggcg gccacaacgc cggtcacacc ctcgtggtcg acggcgagag cttcgccctc
      241 cagctctgcc cctccggcat cctctacgac cacatcacgc cggtcatcgg caacggcgtc
      301 gtggtcgacc cccgcgtgct catcgccgag atggacatgc tcaccagcaa gggcgtcgac
      361 ccgacccggc tcaaggtgag cggcaacgcc cacctgatcc tcccgtacca ccaggagatc
      421 gaccgcctca ccgagcgccg cctgggcagc cggaagctcg gcaccaccaa gcgcggcatc
      481 ggccccgcct acgccgacaa gtcctcccgc gtcggcctgc gggtccagga cctgctcgac
      541 ccgaagatct tccgggccaa gctcgaggtg ctggccaagg agaagaacgc catcttggcc
      601 aaggtcttca accagctgcc cctggatccc gaggagatcg ccgacgagta cctcgacgtg
      661 tgccgcccgc ggatcgaacc gcacatcgcc gacaccgtgt cgctcgtgca cgaggcgctc
      721 gagcgtcagg agcacgtgct cttcgagggg gcgcaggcca cgttcctcga cctcgaccac
      781 ggcacctatc cgttcgtgac gtcatcgaac ccggtggccg gcggcgtctg caccggcgcc
      841 ggggtcgggc cccgctacat cgaccgcgtg atcggtgtgg ccaaggccta cgtgacccgc
      901 gtcggcaccg gcccgttccc caccgagctg gccatctccg gcgaggccgt cggcggcaag
      961 gaccgagagc tggccgacga ggaggagatc cgcctcggcg accacttcgt ggacgtgggc
     1021 cacgagtacg gcaccaactc gggccggcgc cgccgtccgg gctggttcga cgccgtgatg
     1081 ctgcgccagg ccgtccgcct caactcgctg tcggagctgg ccatcaccaa gctcgacatc
     1141 ctcgacacgc tcgacacggt gaaggtgtgc gtcgcctacg acgtgcgcgg tgagcgggtg
     1201 acgcacctgc cgtaccacca gagcgacctc cacgcggcca cgccgatcta cgaggagctt
     1261 cccggctgga agaccgacct gtcggccatc acggagaccc acgagctccc gccggcggcc
     1321 gaggcctacc tcgccttcct cgaggagcag gtcggcgtcc cggtgaccct ggtcggcgtc
     1381 ggccccggcc gcgagcagtt cgtccaccgc aacgcctagc tgggctcgca cgtcagcccg
     1441 acggggccga cggggccgct cgtgtgccat cgtgagcggc ggtcaggtgc ggatcgatcc
     1501 agggagcggt tcatgaaggt cgtcgtcgtc ggttccggtg ggcgcgagca cgcgctggcc
     1561 gtcgtgctcg gccgcaccgc ggaggtggtg gtcacgcctg gcaacccgct gatccccggg
     1621 tcggtcggca cggacgtcga ggacctcgtg gccgacggct cggtcgacct cgtggtcgtg
     1681 gggcccgagc agccgctggt cgccgggctc gccgaccgtc tgcgggccgg tggcgtccgg
     1741 gtgttcgggc cgggggccga gggcgccaag ctcgagggct cgaaggcgta catgaagatg
     1801 atcgcggagg aggccggcct gccgacggcg aggtgggagg cgttcaccga tgccgacgac
     1861 gccgtggact acctgaacgc catgaagggc ccctacgtgg tgaagaccga cggcctggcg
     1921 gctggcaagg gcgtcctcgt caccgactcg ctcgatgacg cggtcagcga cgtccgggcc
     1981 aagctcggcg gcgacacgtt cggcgaatcc ggccgccggg tcgtgatcga ggaggcgctc
     2041 accggtcccg aggtgtcggt gttcgcggtg tgcgacggga cgcgcgccgt gtgcctgccg
     2101 gcggcgcagg acttcaagcg cgtcggcgac ggcgatgccg ggctgaacac ggggggcatg
     2161 ggggcgtggt cgccgctgcc gttcctgccc gacggcttcg aggagcaggt ggcccgggac
     2221 ttcatcgccc ccacgctcgc cgagctgcag caacggggga tcgactaccg gggcgtgctc
     2281 tacgccgggt tcatcctcac ggacgaaggg ccgaagctgc tcgagtacaa cgtgcgcttc
     2341 ggggacccgg actcgcaggt cgtgctgctg cgcatgacgt cggacctcgc cgggctgctg
     2401 gcggcggcgg ccgacggcga tctggcgaag gcgcccgagc ccacgttctc cgacgacgct
     2461 gccgtgctgg tggtggcggc gagcgagggc taccccgtcg tcccccgaac cggcgacgcg
     2521 gtcgagggcc tcgacgccgc cgcgtcggtg gtgggcgtga cggtcctcgg cgccggggtc
     2581 ggccgtcgtc tcgacgggac cgtcaccacg gccgggggtc gtgtcttgaa cgtcgtcggc
     2641 atcggggcga cgaccgacga tgcccgctcg aaggcgtacg gcgcgctcgg ccgcatcacg
     2701 tggcccggcg agcaccaccg caccgacatc gcggcatcgc cggcctgagc cgcctctccg
     2761 aggagacgct ccatcgggca tgatcgctcc atgtggcgtc tgcggggcat cttggcgatc
     2821 tctggcgtgg tggcggccgt tgtgcccctt gcggccgcgg gcgcggcggg cgcggacccg
     2881 gtaccgggcc aggtggtgat caacgagatc cactaccacc ccgtcgtgga cgagcaggag
     2941 ttcctggagc tccacaaccc gggtgacgac ccggtcgact tgagcggcgc ctgcttcacc
     3001 gccggcatcg ggggctgctt cgctgcgggg accacgctgc cggcaggcgg gtacgtcgtc
     3061 gccgcccaat cgctctcggc ctacgccacc gccttcccct cggctcccgc ccccgcggtg
     3121 cag
//
LOCUS       DATMFS010000185         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_146736, whole genome shotgun sequence.
ACCESSION   DATMFS010000185 DATMFS010000000
VERSION     DATMFS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146736"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2438
                     /locus_tag="VNQ33_03430"
     CDS             <1..2438
                     /locus_tag="VNQ33_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWJ97185.1"
                     /translation="LAHASRGHGADFAGIVLIVVGVVAGMGVYAGAGGPVGRGTADLV
                     GFIVGAARVIIPILLVVLGALLVRGAPAPREIDPDDPTVVDLDPQAAEHPFARTVFGG
                     VLIAIAGLGLLHVLRHAPSFSDGADAMADSAGYLGGAIGAPVRAAISTVGAVIVFLAV
                     GFAGVLVITRTTVRHVAGKTAAGVAKGARPVGRQAGAFLNNLFSLGSERAAEGDGEPS
                     LVVGGDIDRRDAEAATAFYDQDQDPDAAPAKPKRTRKPKATLPLPEPDPANPPEQLEI
                     ALPPGTQPSAWKLPPTKLLDLSGSQSVDRKMVEETGRNLEHALAEHGVETRLVGMTVG
                     PTVTRYELELGPGVKVARVTSLHKDIAYAMATPDVRILAPIPGKQAIGVEIPNVRKQL
                     VTVGDILTSSEARAATHPLEVAIGRDIDGKAILANLAAMPHILIAGATGAGKSSCLNS
                     LLTSILMRSTPDQVRMILVDPKRVEMGQYNKLPHLLTEVVTVPKKAANALAWAVREME
                     RRYDLLAECGFRDITGYNAAFDRGDLNDPNAELLGPDAESVTNYQRMPLILVVIDELA
                     DLMMVAARDVEESICRLAQMARAVGIHLVIATQRPSVNVITGLIKANVPARLAFAVSS
                     ATDSRVILDQTGAERLLGKGDMLLLGPSSSIAQRVQGSWVTEDEVRNIAAFWKRQTPE
                     LVYDEAVQGSDEGSGAPGSSGSNGGGAGGGNGDDDDDLLAQATELVVRSQLGSTSMLQ
                     RKLRVGFARAGRLMDELEEKGVVGPSTGSKAREVLMTPEELDGGGDSSGGGGEFDVDE
                     PLNPEDAAGPVIP"
     gene            2701..3210
                     /locus_tag="VNQ33_03435"
     CDS             2701..3210
                     /locus_tag="VNQ33_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HWJ97186.1"
                     /translation="MKKLFSFPNPVNEVAARLVAGGVVLLTLVILLGQTWAIGLLAYG
                     FVARVLTGPTLSPLGQFATRVLAPALPFEAKMVPGPPKRFAQGIGAALSVAAAISYFA
                     FANTTVALVLVAMITVAATLESVFAFCLGCQIFAGLMKLGVIPESVCEECANIWARIP
                     DPSKDTAAV"
BASE COUNT          466 a         1226 c         1109 g          475 t
ORIGIN      
        1 cgctggccca cgccagccgc ggtcacgggg ccgacttcgc cggcatcgtc ctcatcgtgg
       61 tcggcgtggt cgccggcatg ggggtctacg ccggtgccgg tggtcccgtc ggccgcggca
      121 ccgccgacct cgtcggcttc atcgtgggcg cggcacgggt catcatcccg atcctgctcg
      181 tggtcctcgg tgcgctcctc gtgcgcgggg ccccggcacc gagggagatc gaccccgacg
      241 atccgacggt ggtcgacctc gacccgcagg ccgccgagca cccgttcgcc cgcaccgtgt
      301 tcggcggcgt gctcatcgcc atcgccggcc tcggcctgct gcacgtcctg cgccacgcgc
      361 cgagcttctc cgatggcgcc gatgccatgg cggactccgc tggctacctg ggcggcgcga
      421 tcggcgcacc cgtccgggcc gccatcagca cggtgggggc ggtcatcgtc ttcctcgccg
      481 tcggcttcgc cggcgtgctg gtcatcacgc ggaccacggt ccggcacgtg gccggcaaga
      541 ccgcggccgg cgtcgccaag ggtgcccggc cggtcgggcg ccaggccggt gcgttcctca
      601 acaacctctt ctcgctcggc agcgagcggg cagccgaagg cgacggcgag ccgtcgctcg
      661 tggtcggggg cgacatcgac cggcgcgacg ccgaggccgc cacggcgttc tacgaccagg
      721 accaagaccc cgacgccgcg ccggccaagc ccaagcgcac ccgcaagccc aaggccaccc
      781 tgcccctgcc cgagccggat ccggccaacc cgcccgagca gctggagatc gccctgccgc
      841 cgggtacgca gccgtcggcc tggaagctgc cgcccaccaa gctgctggac ctgtcgggca
      901 gccagagcgt cgaccgcaag atggtcgagg agaccggccg caacctcgag cacgccctgg
      961 ccgagcacgg ggtggagacc cgtctcgtcg gcatgaccgt cggccccacc gtcacccgct
     1021 acgagctgga gctcgggccg ggcgtgaagg tggcccgggt caccagcctc cacaaggaca
     1081 tcgcctacgc catggccacg cccgacgtgc gcatcctggc gcccatcccg ggcaagcagg
     1141 ccatcggcgt cgagatcccc aacgtccgca agcagctggt caccgtgggc gacatcctca
     1201 ccagctccga ggctcgggcg gccacccacc cgctcgaggt ggccatcggg cgagacatcg
     1261 acggcaaggc catcctcgcc aacctcgccg ccatgcccca catcctcatc gccggtgcca
     1321 cgggcgccgg caagtcgtcg tgcctcaact cgctgctgac gtcgatcctc atgcggtcca
     1381 cgcccgatca ggtccgcatg atcctcgtgg acccgaagcg ggtcgagatg gggcagtaca
     1441 acaagctgcc ccacctgctc accgaggtgg tcaccgtccc gaagaaggcc gccaacgcgc
     1501 tggcgtgggc cgtgcgggag atggagcgcc gctacgacct gctggccgag tgcggcttcc
     1561 gcgacatcac cggctacaac gccgccttcg accgaggcga cctcaacgat cccaacgccg
     1621 agctgctcgg ccccgacgcg gagtcggtca cgaactacca gcggatgccg ttgatcctcg
     1681 tggtgatcga cgagctggcc gacctgatga tggtcgcggc ccgagacgtg gaggagtcga
     1741 tctgccgcct ggcgcagatg gcccgtgccg tcggcatcca cctcgtcatc gccacccagc
     1801 ggccgtcggt caacgtcatt accggcctca tcaaggccaa cgtgccggcc cgtctcgcct
     1861 tcgccgtgtc gagcgccacc gactcccggg tcatcctcga ccagacgggg gccgagcgcc
     1921 tcctcggcaa gggcgacatg ctcctgctcg gcccgtcctc gtccatcgcc cagcgggtgc
     1981 agggctcgtg ggtcaccgag gacgaggtcc gcaacatcgc cgcgttctgg aagcgccaga
     2041 cccccgagct ggtctacgac gaagctgtcc agggctccga cgagggatct ggcgcgccgg
     2101 gcagctcggg cagcaacggc ggcggcgctg gtggcggcaa cggtgacgac gacgacgacc
     2161 tgctcgccca ggccaccgaa ctggtggtcc gcagccagct cggctccacc tccatgctcc
     2221 agcgcaagct gcgcgtcggg ttcgcccgcg ctggccgcct gatggacgag ctcgaggaga
     2281 agggcgtcgt cgggccgtcg acgggctcca aggcccgtga ggtgctgatg acgcccgagg
     2341 agctcgacgg cggaggcgac agcagcggcg ggggagggga gttcgacgtc gacgagcccc
     2401 tgaaccccga ggacgccgcc ggccccgtca tcccctgacg gcgccctcgg cacgggtgac
     2461 caccccgccc ccgcctcgcc cccgcctcgc ccgggaaccc cggtcgccac cagtctccaa
     2521 gttggaggat gttggcgctc aacgacgatc ttcctccaag ttggagaaac ggggcgggcg
     2581 ggccgtggaa gttggaggat gatggaccga gagcggcgat catcctccaa cttcggcggg
     2641 agacggcgtg gctgcgtcga ggtggtggca attcctgacc tactgggtag ggttttggtt
     2701 gtgaagaagc tgttctcctt cccgaacccg gtcaacgagg tcgcagcccg gctggtggcc
     2761 ggcggcgtcg tcctgctgac cctggtgatc ctgctgggcc agacgtgggc catcggcctc
     2821 ctggcctacg ggttcgtcgc ccgggtcctc accggcccga ccctgagccc gctcggccag
     2881 ttcgccaccc gggtcctggc cccggcgctc ccgttcgagg cgaagatggt ccccggcccg
     2941 cccaagcgct tcgcccaggg catcggtgcc gccctgtccg tggcggccgc catctcgtac
     3001 ttcgccttcg ccaacaccac cgtggccctc gtcctcgtgg ccatgatcac cgtggccgcc
     3061 acgctcgaga gcgtgttcgc cttctgcttg ggctgccaga tcttcgccgg cctcatgaag
     3121 ctcggcgtga tccccgagtc ggtgtgcgag gagtgcgcca acatctgggc ccgcatcccc
     3181 gacccgtcca aggacaccgc cgccgtctga ccggggcggt ggcgggcgtc agcgggtggt
     3241 ggtgcgggtg gccttggtga agtcgagctt cagcgt
//
LOCUS       DATMFS010000186         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53140, whole genome shotgun sequence.
ACCESSION   DATMFS010000186 DATMFS010000000
VERSION     DATMFS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53140"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VNQ33_03440"
     CDS             <1..341
                     /locus_tag="VNQ33_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97187.1"
                     /translation="SSLAIFSAYWLADVENLSKHERQTAATIAALCVSLWILAVLARP
                     YNRWKLALIWAMAGIAVAAVVIPFVRHFFELDIPASFLPQALAIGAAGAVGIELVGRL
                     SSRWSDRSHG"
     gene            354..1430
                     /locus_tag="VNQ33_03445"
     CDS             354..1430
                     /locus_tag="VNQ33_03445"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWJ97188.1"
                     /translation="MDHPILLALSMAFAVAAAVWARSALRLRKVAGRAEREFGRTSDA
                     ATVARSSFRKDVHSTVLYAVLSFVTLGGAFLESGSIWLLTLVLIPVVVSVVYGRDFVR
                     DSRLAESRLELERRAEEVLSQDDLAPKRWAERLAPEHLPDISGFEVGRVYQAGTGMMA
                     GDFYDVFRVAPTRVVALIGDVTGHGIEPSITAFQAKYLLRVFLRQFRDPAQALEELNA
                     QLSALDRDEEMISLCVVVFDTDAGTIRYASAGHPAVWLWHEKDVRPLRSTGPLLMLDP
                     RGTFHSRELRLDSGDLVLLYTDGLSEARAGDQLFGEERIANAIRRDPGADPDVLVKSL
                     LEQARDFARQPITDDIAILALRRD"
     gene            1715..>2780
                     /locus_tag="VNQ33_03450"
     CDS             1715..>2780
                     /locus_tag="VNQ33_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97189.1"
                     /translation="MAQLDVPVPVLPVVDDHGTAWFLSARPDDNGSYLVTAADPDGWV
                     RTSTFQVGGSPNTESTLIMAPSGSGVLASATRCRGDGCVDSDLVVARYERRANDIATV
                     GTPWETPRDRRSSGAAPRPVATTGGVAVLQDSYQLLEFREGMSPDRATLPDNAGDPCL
                     TADGLIVAVEVRPGRSDTGGTGQPTVVTPSQQTADNAVLRLLYLDDQEWSTAAGSERS
                     WASTYRTGAAFHCGAEGPYYALASQAAELAWSDGWNEIRPVEVTWDLYARSTARGPLG
                     VRDGSLTLLDPISSTAASLADVPLPTSVSAALDEAVAASSGQREDPVSGWLAIGGSKS
                     TTGVVCLRAANEHENEVTCYV"
BASE COUNT          399 a          969 c          918 g          494 t
ORIGIN      
        1 tctcctcgct cgccatcttc agcgcctact ggctggcgga cgtcgagaac ctgtcgaagc
       61 acgagcgcca gaccgcggcc accatcgccg ccctctgcgt gtcgctgtgg atcctggcgg
      121 tcctcgcccg gccctacaac cggtggaagc tggcgctgat ctgggcgatg gccgggatcg
      181 ccgtggccgc cgtcgtcatc ccgttcgtcc ggcacttctt cgagctcgac atcccggcgt
      241 ccttcctgcc ccaggccctg gccatcggcg ccgccggcgc ggtgggcatc gaactcgtcg
      301 gccggctctc ctcccggtgg tccgaccgga gccacgggta gcatctccgc accatggacc
      361 atccgatcct cctggcgttg tcgatggcct tcgccgtggc ggcggcggtc tgggcccgct
      421 cggcgctgcg cctccggaag gtggccgggc gcgccgagcg cgagttcggc cgcacctccg
      481 acgccgcgac ggtggcgcgc tcctcgttcc gcaaggacgt gcactccacg gtcctctacg
      541 ccgtgctcag cttcgtcacc ctgggcggag cgttcctcga gagcggctcg atctggctcc
      601 tgacgctcgt gctcatcccc gtcgtcgtct ccgtggtgta cggacgcgac ttcgtccggg
      661 actcccgtct ggccgagagc cggctggagc tcgagcgccg ggccgaggag gtcctgagcc
      721 aagacgacct cgcccccaag cgctgggccg aacgcctggc gccggagcac ctccccgaca
      781 tctccgggtt cgaggtcggc cgggtgtacc aggccggcac cgggatgatg gccggcgact
      841 tctacgacgt gttccgcgtc gcgcccaccc gggtcgtggc gctcatcggc gacgtcaccg
      901 gccacggcat cgagccgtcc atcaccgcct tccaggccaa gtacctgctg cgggtcttcc
      961 tccggcagtt ccgcgatccg gcccaggcgc tggaggagct gaacgcccag ctgtccgccc
     1021 tcgaccgcga cgaggagatg atctcgctct gcgtcgtggt gttcgacacc gacgccggca
     1081 ccatccggta cgcatcggca ggccacccgg cggtctggct gtggcacgag aaggacgtcc
     1141 gcccgctgcg gagcaccggc ccgctcctca tgctcgatcc caggggcacg ttccactccc
     1201 gggagctgcg gctcgactcc ggcgacctgg tgctgctcta caccgacggg ctctccgagg
     1261 cccgcgccgg agaccagctc ttcggcgagg agcgcatcgc caacgccatc cgccgcgacc
     1321 ccggtgctga ccccgacgtc ttggtgaagt cgctgctgga gcaggcccgt gacttcgccc
     1381 gccagcccat caccgacgac atcgccatcc tcgccctccg ccgcgactga cgacccaccc
     1441 aggatcgtat gagggatccg atcatcccct tcgagacctg cgacatcggc ggcaacgcca
     1501 tcctccccta ctgacgtccg gcgctctgct agcctttccg gcgctctgct agcctttctt
     1561 tgaagaccaa ttctgagggg gcggggcgat gaacgcgacg tggaaggctg cgtggctcat
     1621 cgcggcgtgt ggagcattgt tggtcgggtg cggtcagacc gagccgctgc cccatgctca
     1681 ggcaccatcg aacgccagca gcgctggagg ggcgatggcc cagctggacg tgccggtgcc
     1741 agtgcttccg gtcgtcgacg atcatggaac cgcttggttt ctctccgctc gcccggatga
     1801 caacggctcg tacctggtga cggcggccga tcctgatggt tgggttcgca cgtcgacgtt
     1861 ccaagtcggt ggatccccca acacggagtc gacgctgatc atggcgccgt cggggagcgg
     1921 tgttctcgcc agcgccacgc gttgccgggg tgatgggtgc gtcgactctg acttggtcgt
     1981 ggctcgatac gagagacggg ccaacgacat cgccaccgtt ggcacgccat gggagacgcc
     2041 acgcgaccgc aggtcatccg gggcggcacc tcgcccggtc gctacgacag gcggtgttgc
     2101 ggttcttcag gacagctatc aactacttga gttccgcgag gggatgtcgc cggatcgtgc
     2161 cacattgccg gacaacgcgg gcgatccttg cttgaccgcc gacggcttga tcgttgccgt
     2221 ggaggtacgc ccaggtcgta gtgataccgg cgggacggga cagccaaccg tcgtcacccc
     2281 ttcgcagcag acggcggaca acgcggtgct caggctgctc tatctcgatg accaggagtg
     2341 gtccactgct gctggctcgg agcgatcctg ggccagcacg tatcgaacgg gggcggcgtt
     2401 ccactgcggc gctgaagggc cgtactacgc actcgcctcg caagctgccg agttggcttg
     2461 gagcgacggt tggaacgaga ttcgtccggt tgaggtgacg tgggatctgt acgcgcgatc
     2521 caccgctcga gggccgctcg gagtccgaga tggatcgctg acgctgttgg atccaatctc
     2581 gagtacggcg gcttcgctcg ctgatgtacc tctaccaacg tcggtatccg ctgcgctcga
     2641 tgaggccgtt gccgcgtcga gcgggcaacg agaagaccca gtcagtggct ggctggccat
     2701 tggcgggtcg aagtcgacca ccggtgttgt gtgccttcgc gccgccaatg agcatgagaa
     2761 cgaggttacc tgctatgtgt
//
LOCUS       DATMFS010000187         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_193850, whole genome shotgun sequence.
ACCESSION   DATMFS010000187 DATMFS010000000
VERSION     DATMFS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193850"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNQ33_03455"
     CDS             complement(<1..697)
                     /locus_tag="VNQ33_03455"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HWJ97190.1"
                     /translation="MRQPSGWRLALALTAVTFCLSACWALLTPVFHGPDEPEHYDLIS
                     SLASNPHYPEYDQRGLDPTVDDLAQAMLNVGGGSRAPHLGAATPPPILASADLSDPPT
                     GAVRPASNQMPQHPPLYYESMAAALRTMRLVDPGARAPAIASEVVFLRLLNAAVACTI
                     PAAAMAALRLLSPRRTSGIAAGLACLVVPQFSHISGSINNDNLMNLFAAWSIVALLSI
                     LRRGLTVRRAVAAG"
     gene            complement(841..1893)
                     /locus_tag="VNQ33_03460"
     CDS             complement(841..1893)
                     /locus_tag="VNQ33_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWJ97191.1"
                     /translation="MSLATPRAYRMTAWEQPPALVEVPVPVPAAGQVVVRVAGCGLCH
                     SDVAMMAMPAAVGEGLGWQIPFTLGHESAGWVYEIGEGVTAVAVGDAVAVASPASCGT
                     CRWCREGRENACADGLVGRGYGRDGGLADLLVVDDVRALLPTGALDPVLAGPLTDAGA
                     TSHHAVRRVLPRLRDEGTVVVLGVGGLGGFAVQILRALSPARIVAIDPDPARRELAVL
                     YGADEVLDGVTEGTGRQLRAVLGPDGADAVLDVVGTDATIACGVRALASGGAFGLVGA
                     AGGTLRKPWYGTLPRDGELFTFQGSDLADARAVLDLAAAGRISVDIVPFSLDDVDDAY
                     AALGTGGLTGRIVVQP"
BASE COUNT          330 a          847 c          835 g          250 t
ORIGIN      
        1 ctccggccgc gaccgcgcgg cggaccgtca ggcctcgccg gaggatcgac agcagggcga
       61 cgatcgacca cgccgcgaac aggttcatca ggttgtcgtt gttgatcgaa cccgagatgt
      121 ggctgaactg cggcaccacc aggcaggcga ggccggcggc gatgccgctc gtgcgccgcg
      181 ggctgagcag ccgcagcgcg gccatggccg ccgccgggat cgtgcacgcg accgccgcgt
      241 tgaggagccg gaggaacacc acctcgctgg cgatcgccgg ggctcgagca cctgggtcga
      301 ccaaccgcat cgtcctcagg gccgccgcca tcgactcgta gtagagcggc gggtgctggg
      361 gcatctggtt gctcgccggg cgcacggcac cggtgggggg atcgctcagg tcggcagaag
      421 ccaggatcgg cggaggcgtg gcggcgccga ggtgcggggc acgcgatccg ccgccgacgt
      481 tcaacatggc ctgggcgagg tcgtcgacgg tggggtcgag gccgcgctgg tcgtactcgg
      541 ggtagtgcgg gttgctcgcc aacgaggaga tcaggtcgta gtgctcgggc tcgtcaggcc
      601 cgtggaacac aggcgtgagc agggcccagc acgcgctcag gcagaaggtc acggcggtca
      661 gggcgagcgc gaggcgccag ccgctcggct gcctcaccga cgcccgctgc cgggaagcga
      721 cggaccaacg gcgccgtccg aggtccgcgc cgccgcgcgc cggcgaggga accggtctcc
      781 gatcatcggc tcgatcctac gagtcgctcg ccgctcgacc agagtccggc ggcgatccgc
      841 tcagggctgg acgacgatgc gcccggtcag gccgccggtg ccgagggcgg cgtacgcatc
      901 gtcgacgtcg tccagcgaga aggggacgat gtcgacggag atgcggcccg cggccgccag
      961 gtcgagcacg gcgcgggcgt cggcgaggtc cgacccctgg aacgtgaaca gctcgccgtc
     1021 tcgcggcaag gtgccgtacc agggcttgcg gagcgtgccg cccgccgcgc ccacgaggcc
     1081 gaacgctccc ccggaggcca gagcccggac accgcacgcg atcgtcgcgt cggtgccgac
     1141 cacgtcgagc acggcatcag cgccgtcggg tccgaggacc gcccgcaact ggcgcccggt
     1201 cccctccgtc acgccgtcga gcacctcgtc ggcaccgtag aggacggcga gctcccgccg
     1261 agccgggtct ggatcgatgg cgacgatccg agccggcgac agcgcccgca ggatctggac
     1321 cgcgaagccg ccgaggccgc cgacgccgag caccaccacg gtgccctcgt cgcgcaggcg
     1381 gggcagcacg cgcctgaccg catggtggga ggtggctccc gcatcggtga gcggccccgc
     1441 gagcaccggg tcgagggccc cggtgggcag cagggcgcgg acgtcatcga ccaccaggag
     1501 gtcagccagt cctccgtcgc gcccgtagcc ccgcccgacg aggccgtccg cacaggcgtt
     1561 ctcccggccc tcccggcacc aacggcacgt cccgcacgac gccggcgagg cgacggcgac
     1621 cgcatcgccc accgccaccg cggtgacccc ctcgccgatc tcgtacaccc agccggcgga
     1681 ctcgtggccg agcgtgaagg ggatctgcca gccgagccct tcgcccaccg ccgccggcat
     1741 cgccatcatc gccacgtccg agtggcacag gccgcagccg gccacccgga ccacgacctg
     1801 gcccgccgcc ggcaccggca ccggcacctc gacgagggcg ggcggttgct cccacgccgt
     1861 catccggtag gcgcgcggcg tggcgagtga catcggcgcc accgtacgcg acccaggcac
     1921 gcacgagtcc ggctgccgcg tgccggccgg gctcgtctgg gtcgtcgcga ggcggatgaa
     1981 cacctcctgg acgatccccc cggcgggcgt ctcgtcgttc aggaggcgga gggcgagtcc
     2041 gaacgccgcc ccggcgaggc accgctgcgc ctcagcgggg acgtctcggt cgccgcgcca
     2101 ggccgccaac gccagggctg cgccgcggac cacggggacg tcgccgtcac cccgccccgg
     2161 tatcggcccc ggcgcgcttg gcgaaccccg acacgacctc gatgtggctg gtgtggccga
     2221 acaggtccac gaccacggac ccgacgtgct cgtagccggc ag
//
LOCUS       DATMFS010000188         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_146892, whole genome shotgun sequence.
ACCESSION   DATMFS010000188 DATMFS010000000
VERSION     DATMFS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146892"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2064)
                     /locus_tag="VNQ33_03465"
     CDS             complement(<1..2064)
                     /locus_tag="VNQ33_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HWJ97192.1"
                     /translation="MQQPGLDLHQPVRTIANPAPRTAPDDIAPAALPRLLDDHIRFLP
                     LVDGRGHLVAVASARRGDLRIGDRVIADDQPTFVIAEIGINHNGSVDLALDLVDRAAE
                     AGADCAKFQLRDLGSLYREGGREGEDLGTQYTLDLLDRFALSEADLFRAFDRCVERGL
                     VPLCTPWDVESLAALERYGLPGYKVASADLTNHDLLAAIAETGKPIIMSTGMSTESEI
                     AESVAVLQRHGAAFALLQANSTYPAPYKDVNLRYLDQLRRIGGGPVGYSGHERGWHIP
                     VAAVALGARIIEKHLTVDRSLEGNDHKVSLLPGELARLVAEIRQVEEAMGTDATRTIS
                     QGEAMNRVTLAKSVVATRDIAVGEIISAGDLSVKSPGRGLQPNRRDELIGRASTRAVA
                     AGDFFYPSDLDDGRVEPRPYRFRRPWGLPVRYHDLQAIIAKARPDFVEFHFSYQDLEL
                     DPAAFIPEVIDSGYAVHAPDLFRGDHILNLAADDDAWWNRSLDELARTLELARALSPS
                     FTKTDLPIVIVSLGGFTADHPLAVTERAARYDRVIAGLQRLDLSGVEVVAQTLPPFPW
                     YLGGQLHCNLFVDPADTAQFARDAGIGLCLDVSHSKLACNHRGASFAEFVEELGPYVR
                     HLHLVDAQGTDGEGIQVGEGDIDWPVLAGQLDRLAPGVGFIPEIWQGHKNDGEGFWIA
                     LDRLEQ"
BASE COUNT          282 a          659 c          848 g          297 t
ORIGIN      
        1 ctgctcgagg cggtcgaggg cgatccagaa gccctcgccg tcgttcttgt ggccctgcca
       61 gatctcgggg atgaagccga cgccgggagc gaggcggtcg agctggccgg cgagcacggg
      121 ccagtcgatg tcgccctcgc cgacctggat gccctcgccg tcggtcccct gggcgtcgac
      181 caggtggagg tggcggacgt aggggcccag ctcctcgacg aactcggcga agctggcgcc
      241 gcggtggttg caggccagct tcgagtgcga cacgtcgagg cacaggccga tgccggcgtc
      301 gcgggcgaac tgggcggtgt cggccgggtc gacgaacagg ttgcagtgca gctggccgcc
      361 caggtaccag gggaacggcg gcagcgtctg ggccacgacc tcgacgccgg acaggtcgag
      421 gcgctggagc ccggcgatga cgcggtcgta gcgggcggcg cgctcggtga cggccagcgg
      481 gtggtcggcg gtgaagccgc cgaggctgac gatcacgatc ggcaggtcgg tcttcgtgaa
      541 cgacggggag agggcgcggg ccagctcgag cgtgcgggcc agctcgtcga gcgagcggtt
      601 ccaccacgcg tcgtcgtcgg cagccaggtt gaggatgtgg tcgccgcgga acaggtcggg
      661 ggcgtggacg gcgtagccgc tgtcgatcac ctcggggatg aacgccgccg gatccagttc
      721 gaggtcctgg tacgagaagt ggaactcgac gaagtccggg cgggccttgg cgatgatggc
      781 ctgcaggtcg tggtagcgga ccggcaggcc ccacggccgc cggaaccggt acggccgggg
      841 ctcgacgcgg ccgtcgtcga ggtccgacgg gtagaagaag tccccggccg cgacggcccg
      901 ggtgctggcc cggccgatca gctcgtcgcg gcggttgggc tggaggccgc ggcccgggct
      961 cttcacgctg aggtcgccgg cgctgatgat ctcgccgacg gcgatgtccc gggtggcgac
     1021 gacgctcttg gcgagggtga cccggttcat ggcctcgccc tggctgatgg tgcgcgtggc
     1081 gtcggtgccc atggcctcct cgacctggcg gatctcggcc accaggcggg ccagctcgcc
     1141 gggcaggagg ctgaccttgt ggtcgttgcc ctcgagcgag cggtcgacgg tcaggtgctt
     1201 ctcgatgatg cgggcgccga gggcgacggc ggcgacgggg atgtgccacc cccgctcgtg
     1261 ccccgagtac ccgaccggtc cgccgccgat gcgccggagc tggtcgaggt agcggaggtt
     1321 cacgtccttg tagggcgcgg ggtaggtgct gttggcctgg agcagggcga aggcggcgcc
     1381 gtggcgctgg agcacggcga cggactcggc gatctcggac tcggtggaca tgccggtgga
     1441 catgatgatc ggcttgccgg tctcggcgat ggccgccagc aggtcgtggt tcgtgaggtc
     1501 ggccgaggcc accttgtagc cgggcagccc gtagcgctcc agcgcggcga ggctctcgac
     1561 atcccacggc gtgcacagcg gcaccaagcc gcgctcgacg caccggtcga acgcgcggaa
     1621 caggtcggct tcggacaggg cgaagcggtc gagcaggtcg agcgtgtact gggttcccag
     1681 atcctcgccc tcgcgtccgc cctcgcggta gagggagccc aggtcgcgga gctggaactt
     1741 ggcgcagtcg gccccggcct cggcggcgcg gtcgaccaga tcgagagcca ggtcgacgct
     1801 gccgttgtgg ttgatgccga tctcggcgat gacgaaggtc ggctggtcat ccgcgatcac
     1861 ccggtcgccg atccgcaggt cgccgcggcg ggccgaggcg acagcaacca ggtggccgcg
     1921 cccgtcgacc agcggcagga agcggatgtg gtcgtcgagc agccggggca gggcggcagg
     1981 ggcgatgtcg tcgggcgccg tgcggggcgc cgggttggcg atggtccgca ccggctggtg
     2041 gaggtcgagg ccgggctgct gcacgaccca gcggcggaag tcgccg
//
LOCUS       DATMFS010000189         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_217088, whole genome shotgun sequence.
ACCESSION   DATMFS010000189 DATMFS010000000
VERSION     DATMFS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217088"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VNQ33_03470"
     CDS             complement(<1..1033)
                     /locus_tag="VNQ33_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ97193.1"
                     /translation="MLDAALASQLQTHLEKLRQPIELVTSLDDSPKSTELRELLDQIA
                     SLSGKVEVIAADDDDRRPSFLIRRTGTDVEVRFAGIPLGHEFTSLVLALLQVGGHPST
                     ADDDVIQQVMDLEGEHHFETYFSLTCQNCPDVVQALNLMSILNPNITHTAIDGALFQD
                     EVDERKVMAVPSVFRNGEPFDQGRMSLEQIVAKLDTGAAERVAAELAGKDPFDVLVVG
                     GGPAGAASAVYASRKGIRTGIAAERFGGQVLDTMAIENFISVPYTEGPKLATALEQHV
                     HQYDVDVMNLQRAAKLVPAAEPGGYVTVELASGASLSSRTVVLSTGARWRAMNVPGED
                     EYRNKGVTYC"
     gene            complement(1154..1717)
                     /gene="ahpC"
                     /locus_tag="VNQ33_03475"
     CDS             complement(1154..1717)
                     /gene="ahpC"
                     /locus_tag="VNQ33_03475"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HWJ97194.1"
                     /translation="MSLVNTEIKPFSTTAFHNGEFVDVTDADLKGSWSVVFFYPADFT
                     FVCPTELGDLADNYEEFKRLGVEVYSVSTDTHFTHKAWHGSSDTIGKIQYPMLGDPTL
                     EISRNFEILREGQGLADRGTFLVDPEGVIQFLEVTAEGIGRNAVELLRKVKAAQYVAA
                     HPGEVCPAKWEEGDDTLTPSLDLVGKI"
     gene            1814..3367
                     /locus_tag="VNQ33_03480"
     CDS             1814..3367
                     /locus_tag="VNQ33_03480"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HWJ97195.1"
                     /translation="MPLIATVDRIRRSEAGAEPRQGGQGYGAAIDRRQFLQRAIVAGA
                     GAAVIPSAMVSAQPATPGAGPYGGLAAEPDENGLHLPEGFTSRILAVGGETVAGTGYT
                     WHAFPDGAATFATDDGGWIYACNSEVTAVFAPDQGGVSAIRFDADGEVVDAYRILEGS
                     TSNCAGGLTPWGTWLSCEEPVDKRGRLWECDPTGEQEAVAHEAMGLWNREAAAVDPER
                     KAVYMTEDDPEGLLYRYTPAAYPDLSNGDVHAAVVADNGAVTWAEVPDPSASSKPTKA
                     QVDGATTFNGAEGIWYDRGSIYFTSKGDNKVHHIDLATQRYEVIWDSDPASEGKEGAV
                     LSGVDNITVDHATGDLFVAEDGGNMEVVLISADGTVAPFARVAEDPTGSEITGPCLSP
                     DRTRLYFSSQRGPSPKMLKEVIPGCTFDARNAGITYEITGPFRTAAAEPEPVATTIAK
                     ANPSGAPNSSAGSDSGEGESTSAPVVIGGIALVAAAGVGATIALRRRRGASGSPATPP
                     ADEDAAPGA"
     gene            complement(3378..4409)
                     /locus_tag="VNQ33_03485"
     CDS             complement(3378..4409)
                     /locus_tag="VNQ33_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWJ97196.1"
                     /translation="MSDSDAVAPDTAVALLATLAGASGSEGLAYAAPPVSLQGGYYAE
                     MIRFRLADPPEGLAGDLVARIVPNPVVAAWESALQRDVARQGFPTPSVRLVADDRTPL
                     GRSLLVMDHVEGRSTLSGLHGIGIVTQIPAVVRHLPDQLAALAARLHALDAHAVTAEL
                     EALDGGIPTTTKGFVEGELWRAAVADRPDLVAVAEQLIATEPPSARRAISHGDLHPFN
                     VLVTASGPVLIDWTVARVAHPAFTLGFTDLMLSNPPLEVPSALGGGLRALGRSLARRF
                     HATYRSLAQPADHVSAEELDWHRKVHALRILVELAGWDAAGARPASHPWLILEPVTER
                     LLGITTRTR"
     gene            complement(4430..>4803)
                     /locus_tag="VNQ33_03490"
     CDS             complement(4430..>4803)
                     /locus_tag="VNQ33_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ97197.1"
                     /translation="VTVVTWGATVAKSVAAADQLAEQEGIEAEVIDLRTILPWDHGIV
                     AGSAARTSRVLVVHEDTLTAGFGGEIAAWVGEHCFADLDAPVRRIGATDTPVAYEPAL
                     EDAILPQVTDIATALLDLATW"
BASE COUNT          718 a         1572 c         1842 g          671 t
ORIGIN      
        1 ggcagtacgt cacgcccttg ttgcggtact cgtcctcgcc gggcacgttc atggcccgcc
       61 agcgggcacc ggtcgacagc acgaccgtgc gggaggagag gctggcaccg ctggccagct
      121 cgacggtgac gtagccaccg ggctcggcag cgggaacgag cttggcggcc cgctgcaggt
      181 tcatcacgtc gacgtcgtac tggtggacgt gctgctcgag cgccgtcgcc agcttcgggc
      241 cctcggtgta gggcaccgag atgaagttct cgatggccat ggtgtcgagc acctggccgc
      301 cgaagcgctc ggcggcgatg ccggtgcgga tgcccttgcg gctggcgtag acggccgatg
      361 ccgcaccggc cgggccgccg ccgacgacga ggacgtcgaa cgggtccttg ccggccagct
      421 cggcggcgac gcgctcggcg gcgccggtat cgagcttggc cacgatctgc tcgaggctca
      481 tgcggccctg gtcgaacggc tcgccgttgc ggaagaccga cgggaccgcc atcaccttgc
      541 gctcgtcgac ctcgtcttgg aacagggcgc cgtcgatggc ggtgtgggtg atgttcgggt
      601 tcaagatgct catgaggttg agcgcctgga cgacgtcggg gcagttctgg caggtgagcg
      661 agaagtaggt ctcgaagtgg tgctcgccct cgagatccat gacctgctgg atcacgtcgt
      721 cgtccgcggt ggacgggtgg ccgccgacct ggaggagggc gaggacgagc gacgtgaact
      781 cgtggcccag cgggatgccg gcgaagcgga cctcgacatc ggtgccggtc cggcggatga
      841 ggaacgacgg gcggcggtcg tcgtcatcgg cggcgatgac ctcgaccttg ccggagagcg
      901 aggcgatctg gtcgagcagc tcgcgcagct ccgtggactt ggggctgtca tcgagcgagg
      961 tgacgagctc gatcggctgg cggagcttct cgaggtgggt ctgcagttgg gaggcgaggg
     1021 cggcgtcgag catggcggca gctccaggag gtcgggtcgg gagggtgcgg aacggaggga
     1081 tcggggcgcg tccgggaaga ccgagggcgg gccgttgcgg cccgccctcg gcttcaccag
     1141 gtggtggggt ggatcagatc ttgccgacga gatccagcga gggggtgagg gtgtcgtcgc
     1201 cctcttccca cttggcgggg cagacctcgc cggggtgggc ggcgacgtac tgggcggcct
     1261 tcaccttgcg gaggagctcc acggcgttgc ggccgatgcc ctcagcggtg acctcgagga
     1321 actggatgac gccctcgggg tcgacgagga aggtgccgcg gtcggccagg ccctggccct
     1381 cgcggaggat ctcgaagttg cgggagatct cgagcgtggg gtcgccgagc atcgggtact
     1441 ggatcttgcc gatggtgtcg gagctgccgt gccaggcctt gtgggtgaag tgggtgtcgg
     1501 tggacaccga gtacacctcg acgccgaggc gcttgaactc ctcgtagttg tcggccaggt
     1561 cgcccagctc ggtggggcag acgaaggtga agtcggccgg gtagaagaac acgaccgacc
     1621 acgagccctt gaggtcggcg tcggtgacgt cgacgaactc gccgttgtgg aaggcggtgg
     1681 tggagaaggg cttgatctcg gtgttgacga gcgacatgtg cgaaggacct ttcggatctc
     1741 gggtgcggtt gagcaatagg cactctatat gaaacccggc ttagatcatc atcttcacct
     1801 attgattctg ccaatgcctc tgatagctac cgtcgatcga atccgcaggt cagaggcggg
     1861 cgcggagccc cggcaaggcg gccagggcta cggtgcggcc atcgaccgac gacagttcct
     1921 ccagcgggcc atcgtggccg gggccggcgc cgccgtgatc ccgtcggcga tggtgtccgc
     1981 ccagcccgcg acaccgggcg caggtcccta cggcgggctc gcggccgaac ccgacgagaa
     2041 cggcctgcac ctgcccgagg gcttcacgtc gcgcatcctc gcggtgggtg gcgagaccgt
     2101 cgcggggacc ggctacacct ggcacgcctt ccccgatggg gccgccacct tcgccaccga
     2161 tgacggcggc tggatctacg cctgcaacag cgaggtcacc gcggtgttcg cccccgatca
     2221 gggcggcgtg agcgccatcc gcttcgacgc cgacggcgaa gtggtcgacg cctaccggat
     2281 cctggagggg agcacctcga actgcgccgg cgggttgacg ccgtggggca cctggctgag
     2341 ctgcgaggag ccggtcgaca agcgaggccg gctctgggag tgcgacccga ccggcgagca
     2401 ggaggccgtc gcccacgagg cgatggggct ctggaaccgc gaggccgccg cggtcgaccc
     2461 ggagcgcaag gccgtctaca tgaccgagga cgaccccgag ggcctgctct accgctacac
     2521 gcctgccgcc taccccgacc tgtcgaacgg cgacgtccac gcagccgtcg tggccgacaa
     2581 cggcgcggtg acgtgggccg aggtccccga tccgtcggcg tcgtccaaac ccaccaaggc
     2641 gcaggtcgac ggggccacca cgttcaacgg cgccgagggg atctggtacg accgcggatc
     2701 gatctacttc acctccaagg gcgacaacaa ggtccaccac atcgacctgg cgacgcagcg
     2761 gtacgaggtc atctgggaca gcgacccggc cagcgagggc aaggaggggg ccgtgctgtc
     2821 cggtgtcgac aacatcaccg tcgaccacgc caccggcgac ctgttcgtgg ccgaggacgg
     2881 cgggaacatg gaggtcgtgc tgatcagcgc cgacggcacg gtcgctccgt tcgcccgggt
     2941 ggccgaggac cccaccgggt cggagatcac cgggccgtgc ctctcccccg accgcacccg
     3001 gctctacttc tccagccagc gcggaccgtc gccgaagatg ctcaaggagg tgatccccgg
     3061 ctgcacgttc gacgcccgca acgccggcat cacctacgag atcaccggac cgttccgcac
     3121 ggcagccgcg gagcctgagc cggtcgccac caccatcgcc aaggccaacc cgtccggcgc
     3181 gccgaacagc tccgccggct cggacagcgg tgagggtgag agcaccagcg caccggtcgt
     3241 gatcggcggg atcgccctcg tcgccgcggc gggggtcggc gcgacgatcg ccctccgccg
     3301 ccgccgtggc gccagcggat cacccgcgac cccgcccgcc gacgaggacg ccgcgcccgg
     3361 agcctgagcg acgggcgtca gcgggttcgg gtcgtgatgc cgaggagccg ctcggtgacc
     3421 ggctcgagga tgagccaggg gtggctcgca ggccgggcgc cggcggcgtc ccagccggcg
     3481 agctcgacga ggatgcgcag ggcgtggacc ttgcggtgcc agtcgagctc ctcggccgag
     3541 acgtggtcgg cgggctgggc gagcgagcgg taggtcgcgt ggaaccgccg agcgagcgat
     3601 cggccgagcg cccggagccc gccgccgagg gcgctcggaa cctccagcgg cgggttcgac
     3661 aacatgagat cggtgaagcc gagcgtgaag gcgggatggg cgacgcgcgc gacggtccag
     3721 tcgatcagga ccgggccgct cgcggtcacc agcacgttga acgggtgcag gtcaccgtgg
     3781 ctgatcgccc gtcgggcgct cggcggctcg gtggcgatca gctgctcggc gacagcgacg
     3841 aggtcggggc ggtccgccac cgcggcgcgc cagagctcgc cttcgacgaa gcccttcgtg
     3901 gtggtgggga tgccgccgtc gagcgcctcg agctcggcgg tgacggcatg ggcgtcgagg
     3961 gcgtggagcc tggcggccaa cgccgccagc tgatccggca ggtggcggac gacggccggg
     4021 atctgggtga cgatcccgat gccgtggagc cctgacagcg tcgagcggcc ctcgacgtgg
     4081 tccatcacga ggagcgaccg gccgagcggc gtgcgatcgt cggcgaccag gcggacggac
     4141 ggcgtgggga acccctgccg ggcgacgtcg cgctggaggg cggactccca ggcggcgacc
     4201 accgggttcg gcacgatgcg ggcgacgagg tcgccggcca gcccttcggg cggatcggcc
     4261 agccggaacc ggatcatctc cgcgtagtac ccgccttgga gcgagaccgg cggagcggcg
     4321 taggcgaggc cctcggagcc gctggcaccg gcgagcgtgg cgagcagggc caccgccgtg
     4381 tccggcgcca ccgcgtcgct gtcgctcacg gtggccaccg taccggcggc taccaggtgg
     4441 cgaggtcgag cagggcggtg gcgatgtcgg tgacctgcgg gaggatggcg tcctcgaggg
     4501 ccggctcgta ggccaccggg gtgtccgtgg cgccgatgcg ccggaccggt gcgtcgaggt
     4561 cggcgaagca gtgctcgccg acccaggcgg cgatctcgcc gccgaagccg gcggtgagcg
     4621 tgtcctcgtg cacgacgagg acccggctgg tgcgggcggc ggacccagcc acgatcccgt
     4681 ggtcccacgg caggatcgtc cggaggtcga tgacctcggc ctcgatcccc tcctgttcgg
     4741 cgagctggtc ggcagcggcg accgacttgg cgacggtggc gccccacgtg acgacggtga
     4801 cgt
//
LOCUS       DATMFS010000190         2822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_238369, whole genome shotgun sequence.
ACCESSION   DATMFS010000190 DATMFS010000000
VERSION     DATMFS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238369"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VNQ33_03495"
     CDS             complement(<1..234)
                     /locus_tag="VNQ33_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97198.1"
                     /translation="MVTDRVRIDSLAAGREHLARAQRVPGSVLVVPIDRTVPAWLGEL
                     AAAAHVVDATVVAEARRPADLVGEAGDGWEIGVC"
     gene            complement(269..2044)
                     /locus_tag="VNQ33_03500"
     CDS             complement(269..2044)
                     /locus_tag="VNQ33_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWJ97199.1"
                     /translation="MTAPERTAALPPALPPAPASAPQLTDRGAVVLAVAVWVSAVAHR
                     PVPALVLVGVVLGVLVVRRPVVLVVAAALLAGTLASRAHGGLHPVAPGPYSGVATLVA
                     DPSPTAFGWRADVRLGHRRVELTAGDAAGGALSAALTGERIRVEGKLAPPRPSSPWLV
                     PRHVVGRLAATSVERVDAGAAPWRAANRFRRLLQRGASTMGERERSLYAGFVLGDDRD
                     QPPEVVDDFRGAGLTHLLVVSGQNVAFLLVLVSPITTRLRWGGRWAVTIAVIAVFGVV
                     TRFEPSVLRASAMAAVAVTAGAVGRPTGSVRTLALAVSGLILVDPLLVHSTGFQLSVG
                     ASLAIAVGASRIARVLPGPGPLADAVGVTLAAQFGVAPVLVPLAGGLPVVSLLANPLA
                     VPVAGLVTTWGLPAGVVAGLGGHRLASVVHLPTELMIRWVAAVARVGASVPLGALGWP
                     HLAGLAVALAVAARPPPLRIAAVPVALVSVLAPAVALRSPPTEVVADGLSVHRAGGAT
                     VVVVDGAPPVDALEVLRTAGVRRIDVLVVDGRGAAELVRVLRHRWSLRRVIDAGSSGP
                     VRLLVGGLDISVEPPAPPVVTARPP"
     gene            complement(2041..>2822)
                     /locus_tag="VNQ33_03505"
     CDS             complement(2041..>2822)
                     /locus_tag="VNQ33_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWJ97200.1"
                     /translation="DPDVDPFWHDAFRRRPPLLERATAMARPMRSWAVAVLGIVVVVG
                     AGWWLLRPGAPPIESALPAAEAAPAPGSAPASGAGRSSPAPAAGASTSTTVAPVVVQA
                     AGAVAKPGVYVLDPGSRVDDLVEAAGGVTEQADVDRVNLAAPVADGERVWVPSRGEET
                     VPEVVAGSGGGGTGGGGAGAAVEAAGGGGGTTPGPSSPVDLNSATAEQLDGLPGVGPT
                     TAAAILAYRDEHGRFGSVEELLDVRGIGEAKLEQLRPLVRV"
BASE COUNT          431 a         1128 c         1027 g          236 t
ORIGIN      
        1 gcagaccccg atctcccagc cgtcgcccgc ctcgccgacg agatcggcgg gtcggcgcgc
       61 ctcggcgacc accgtcgcgt ccacgacatg agcggccgcc gcgagctcac cgagccacgc
      121 cggcacggtg cggtcgatcg ggaccaccag gaccgacccc ggaacccgct gggcacgggc
      181 cagatgctcc cgaccggccg caaggctgtc gatgcgcact cggtcggtga ccacgcacgg
      241 accctaccgc ccgacccacg gcccgggatc agggtggacg ggccgtgacc accggcggtg
      301 cgggcggttc gaccgagatg tcgagcccgc ccacgagcag ccggacgggg ccggacgatc
      361 cggcgtcgat gacccgccgg agggaccacc ggtggcgcag cacccgtacc agctcggcag
      421 cgccccgccc gtcgaccacc aagacgtcga tccgcctcac gccggcagtg cggagcacct
      481 cgagcgcgtc gacgggcggc gcgccgtcca ccacgaccac ggtcgcccca cccgcccggt
      541 gcaccgacag cccgtcggcc accacctccg tgggcggtga gcgcagggcg accgcagggg
      601 cgaggaccga gacgagcgcg acggggacgg cggcgatgcg cagcggaggc ggacgggccg
      661 cgacggccag cgccaccgca agcccggcga ggtgcggcca cccgagggcg ccgagcggga
      721 ccgacgcgcc gacccgggcg accgcggcca cccaccggat catcagctcc gtcgggaggt
      781 ggaccaccga cgccagccgg tggccaccga ggccggcgac gacacccgcc ggcagacccc
      841 aggtggtgac gagcccagct accgggaccg ccagcgggtt ggcgaggagc gacaccaccg
      901 gaagcccgcc agccagcggc accagcaccg gcgccacccc gaactgggcg gccagggtga
      961 cgccgacggc atcggccagc ggtcccggac ccggcagcac ccgggcgatg cgcgaggccc
     1021 cgaccgcgat ggccagcgag gcgccgaccg acagctggaa gccggtcgag tggacgagga
     1081 ggggatccac caggatcagg ccgctcacgg cgagcgccag ggtgcgcacc gacccggtcg
     1141 ggcggccgac cgcgcccgcg gtcacggcca ccgccgccat cgccgaggcc cgcaggaccg
     1201 atggctcgaa ccgcgtcacc acaccgaaca cggcgatcac cgcgatggtc acggcccacc
     1261 gaccacccca ccgcagccgc gtcgtgatcg gcgacaccag gaccaagagg aaggccacgt
     1321 tctgtcccga gacgacgagc aggtgcgtga gcccggcccc acggaagtcg tccacgacct
     1381 ccggcggctg gtcccggtcg tcgccgagga cgaagccggc gtagagcgac cgctcacgct
     1441 cgcccatcgt cgacgcgccg cgctggagca gccgacggaa ccggttggcc gcccgccagg
     1501 gggccgcccc ggcgtcgacc cgctccaccg aggtggcggc gagccggccg acgacgtgcc
     1561 ggggcaccag ccacggcgag ctcgggcgcg gtggcgccag cttgccttcg acgcggatgc
     1621 gctcgccggt gagggccgcg ctgagcgctc cacccgccgc gtcgcccgcg gtcagctcca
     1681 cccgccggtg accgagcctc acgtcggcgc gccacccgaa ggcggtgggc gacggatccg
     1741 ccaccagggt cgcgacaccc gagtacggcc cgggggcgac cggatgcagg ccgccgtggg
     1801 cgcgggaggc gagggtgccg gccagcagtg ctgcggcaac cacgagcacc accggacggc
     1861 ggaccaccag gactcccagc acgacaccca cgagcaccag cgccggcacc gggcggtgcg
     1921 ccacggcaga cacccagacc gcgacggcca ggaccaccgc gccacgatcg gtgagctggg
     1981 gtgccgaggc gggcgccggc gggagcgccg gtggaagggc cgcagtgcgc tcgggtgcgg
     2041 tcacacccgg acgaggggtc ggagctgctc cagcttcgcc tcaccgatgc cccgcacatc
     2101 gaggagctcc tcgaccgatc cgaagcgacc gtgctcgtct cggtaggcga ggatggccgc
     2161 tgcggtcgtc ggaccgaccc cgggaaggcc gtcgagctgc tccgccgtcg ccgagttcag
     2221 atccaccggc gacgacggac cgggggtggt accgccccct cccccggcgg cctccaccgc
     2281 ggcccctgct cccccgccgc cggtaccgcc cccacccgat ccggcgacga cctcgggcac
     2341 ggtctcctca cctcggctcg gcacccacac gcgctcaccg tccgcgacgg gggcggcgag
     2401 gttcacccgg tcgacgtcgg cctgctcggt gacgcctccc gccgcttcca cgaggtcatc
     2461 cacgcgcgaa ccggggtcca gcacgtagac gcccggcttc gccacggctc ccgccgcctg
     2521 caccacgacc ggtgcgaccg tcgtggacgt ggaggctccc gcggcgggcg ccggtgacga
     2581 gcggccggcc ccgctcgccg gagcagagcc cggtgccggc gcggcctcgg ccgcgggcag
     2641 tgcggattcg atgggcggcg cgccgggccg gagcagccac cagcccgcac ccacgaccac
     2701 gacgatgccg agcacggcga cagcccacga ccgcatcggc cgggccatcg ccgtcgcccg
     2761 ctccagaagc gggggccgac ggcggaaggc gtcgtgccag aacgggtcga cgtccgggtc
     2821 gg
//
LOCUS       DATMFS010000191         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_194066, whole genome shotgun sequence.
ACCESSION   DATMFS010000191 DATMFS010000000
VERSION     DATMFS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194066"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VNQ33_03510"
     CDS             <1..117
                     /locus_tag="VNQ33_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97201.1"
                     /translation="TSMTVELIAPIAMDEGLRFAIREGGRTVGAGRVTEILK"
     gene            131..451
                     /gene="rpsJ"
                     /locus_tag="VNQ33_03515"
     CDS             131..451
                     /gene="rpsJ"
                     /locus_tag="VNQ33_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWJ97202.1"
                     /translation="MADAQKIRIRLKAYDHEVIDQSTKKIVETVLRTNATVRGPIPLP
                     TSKHRYTVIRSPHKYKDSREHFEMRIHKRLLDILEPTPKTVDSLQRLDLPAGVDIEIK
                     LQTA"
     gene            complement(530..1636)
                     /locus_tag="VNQ33_03520"
     CDS             complement(530..1636)
                     /locus_tag="VNQ33_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97203.1"
                     /translation="MTATRPVVWAVVRDLGRTGVPIALARLATWPATSEAVDLHLIAR
                     HDGPLRTELERAGIGVTALEPADGRSRAATVAAGASHLGRTDAGAAVLGAAWRRRIRN
                     LPAPDTVLVQGAGAWPVAEALRAEAAPRIVVHLHELEVAVRRSIGPSSLPSLAAADQV
                     LAVSEPVASLARSLGVPDDRLSIVGGTVDLVSSAARGRTDVVSVGEAGWRKGTDRAVA
                     AAHVLRRREPTLRWHWIGRALEPGWAFAVDAPLPILHHAPVADPWSVVHEPAALVVPS
                     REDPLPLVALEAAARSVPVIACRGAGGLDGLLAEGRGWLVDPTDPGDLAATVAEVVAA
                     RPTAGPEALRDHVERHHTLEAVGPRWLHALTGGT"
     gene            complement(1633..2772)
                     /locus_tag="VNQ33_03525"
     CDS             complement(1633..2772)
                     /locus_tag="VNQ33_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97204.1"
                     /translation="MIAVPRVVLVGHDASATGAPLTALAFARWAAASGAAHVEVLLDR
                     GGPLVAGFEAVAPTHVRARAAGEVVAAADALGGLSAGRWARRGALLTARPPTGADVTV
                     VAASVAAWRSAAAVARGGRRLVLWLHELDGVADRVVPASERAILLEATSRIVAVEARV
                     AAMATERWGVAEDRVSVVGSFVDPLGDGAAAGQATAHPPHDVVGVGSLVPRKGADHLV
                     ALTALFRQRGRDLRSAWVGGPLDGPYADLVRTDRAAAGLEDVLDLVGPVDDVGPWWPA
                     SGVVVHLAREDPAPLVVIEAGLRAIPVVTWDTGGAADLLRTAGLGHLVADAGDLVAVA
                     DAVEALLDDGAGRAEAGAALRAAATARTTDRLAPALLQAFTGGRG"
BASE COUNT          395 a         1068 c         1114 g          295 t
ORIGIN      
        1 acgtcgatga cggtggagct gatcgctccg atcgcgatgg atgagggact gcggttcgcc
       61 atccgtgagg gcggtcgcac cgtgggcgcc ggccgcgtca ccgagatcct caagtagaga
      121 cgacgacaac atggcagacg cccagaagat ccgcatccga ctcaaggcct acgaccacga
      181 ggtcatcgac cagtcgacca agaagatcgt ggagacggtg ctgcgcacca acgccacggt
      241 ccggggtccc atcccgcttc cgacgagcaa gcaccgctac acggtgatcc gctcgccgca
      301 caagtacaag gactcccgcg agcacttcga gatgcgcatc cacaagcgcc tgctcgacat
      361 cctcgagccc acgcccaaga cggtcgactc gctgcagcgc ctcgacctcc cggctggcgt
      421 cgacatcgag atcaagctcc agacggcctg atcgccgccc cttccggggg ctgcggttcg
      481 acgaagtccg gtgatgggcg ccttcgggcg cccatcaccg cgtccgggct caggtcccgc
      541 cggtgagggc gtgcagccag cggggcccga ccgcctcgag cgtgtggtgg cgctccacgt
      601 ggtcgcgcag ggcttcgggt ccggcggtgg gccgggcggc gacgacctcg gcgaccgtcg
      661 ccgccaggtc gcccgggtcg gtgggatcga ccagccagcc ccggccctcg gcgaggaggc
      721 cgtcgaggcc tccggcgccg cggcaggcga tgacgggaac ggagcgagcc gcggcctcga
      781 gggccacgag cgggaggggg tcctcccggc tcgggacgac cagcgcggcg ggttcgtgga
      841 cgaccgacca cgggtcggcc accggggcgt ggtgcaggat cgggagcggc gcgtcgacgg
      901 cgaacgccca cccgggttcg agcgctcgcc cgatccagtg ccaccgcagc gtcggctcgc
      961 gccggcgcag gacgtgggcg gcggcgaccg cccggtcggt gcccttgcgc cagccggcct
     1021 cgccgaccga cacgacgtcg gtgcggcccc gtgccgcgct cgagacgagg tcgacggtcc
     1081 ccccgacgat cgacaggcga tcgtcgggga ccccgagcga acgggccagc gatgcgaccg
     1141 gctcggagac cgccagcacc tgatcggcgg cggcgaggga ggggagcgag ctgggcccga
     1201 tgctgcgccg caccgcgacc tcgagctcgt ggaggtggac gacgatccgg ggcgccgctt
     1261 cggcacggag ggcttcggcc accggccagg cgccggcacc ctgcacgagg accgtgtcgg
     1321 gggccgggag gttccggatc cggcggcgcc acgcagctcc gagcaccgcg gcgccggcat
     1381 cggtccgccc gaggtgcgac gcgccggccg caacggtggc ggcacgagat cgcccgtcgg
     1441 ccggttcgag ggcggtgacc ccgatgccgg cgcgctccag ctcggtgcgc aggggcccgt
     1501 cgtggcgggc gatgaggtgc aggtcgacgg cttcgctcgt ggccggccag gtggccagcc
     1561 gggccaaggc gatggggacg ccggtccgcc cgaggtcgcg cacgaccgcc cacacgaccg
     1621 ggcgggtggc ggtcaccccc gtcctccggt gaacgcctgg agcagggcgg gcgccagccg
     1681 gtcggtggtg cgggcggtcg ccgccgcccg gagcgcggcc ccggcctccg ctcggccggc
     1741 gccgtcatcg agcagcgcct cgacggcatc ggccacggcg accaggtcgc ctgcatcggc
     1801 cacgaggtgg cccagcccgg ccgtccgcag gaggtcggcg gcgccgccgg tgtcccaggt
     1861 gaccaccggg atggcccgca ggccggcttc gatgaccacg agcggtgccg gatcctcgcg
     1921 ggccaggtgc acgacgaccc cgctggcagg ccaccacggg ccgacgtcat cgaccggacc
     1981 gaccaggtcg agcacgtctt cgagccccgc agcagcgcgg tcggtgcgga cgaggtcggc
     2041 gtaggggccg tcgagcggtc cgcccaccca ggcgctgcgc aggtcccgac cgcgctggcg
     2101 gaacagcgcg gtgagcgcca cgaggtggtc ggcgcccttg cggggcacga gcgagccgac
     2161 tccgacgacg tcgtgcggcg ggtgcgccgt ggcctgcccc gcggcggcgc cgtcaccgag
     2221 cgggtcgacg aacgagccga cgaccgacac gcggtcttcg gccacacccc accgctcggt
     2281 ggccatcgcc gccacgcggg cctcgaccgc gacgatgcgg gaggtcgcct cgagcaggat
     2341 cgcccgctcg gatgccggga cgacgcggtc ggccacgccg tcgagctcgt ggagccacag
     2401 gaccaggcgg cggccgcccc gggcgacagc agcggccgag cgccaagcgg cgacgctggc
     2461 ggccacgacg gtgacgtcgg cccccgtcgg cggccgggcg gtgagcaggg cgccgcgccg
     2521 ggcccagcgg ccggcggaga gcccgccgag cgcgtcggcc gcggccacca cctcccccgc
     2581 ggcccgggca cggacgtggg tcggggcgac ggcctcgaac ccggcgacca ggggcccgcc
     2641 ccgatcgagc agcacctcga cgtgggccgc gcccgaggct gcggcccagc gggcgaacgc
     2701 cagggcggtg agcggcgcgc ccgtggcgct ggcgtcgtga ccgacgagca cgacccgggg
     2761 cacggcgatc acggcgggca gtatcccacg gaccgcccgg cggccggagg tgagcgccgt
     2821 gttgacgctc ctgtcagcag cgccggagac tacggggctc cggcgtgccc tc
//
LOCUS       DATMFS010000192         4177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_194091, whole genome shotgun sequence.
ACCESSION   DATMFS010000192 DATMFS010000000
VERSION     DATMFS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194091"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..2146)
                     /locus_tag="VNQ33_03530"
     CDS             complement(422..2146)
                     /locus_tag="VNQ33_03530"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97205.1"
                     /translation="MSTATLKNTSPIITPDSGKAGLYPSTINVSGLGTSLLSLKVVVR
                     GANHGCTKDLDVLLVGPDGTKTTLMSDNGAPAIEFDGCTNLQKDSITFDDACPDFANS
                     MPSGANICVRPSDNDALGHQGDSWPDVGDNFPALNLSTFAGKNPNGAWKLYVVDDASS
                     DSGGFNGGWELQLSTNNSPPVANPQVVTAYKGTPVPVVLTGTDPDGDPLTCTAPATTT
                     QGKGTLAGSGCNRTYTAATRTSGTDGFAFKVTDVNGSSSANANVVFNITNRAPTATDQ
                     TVTVGRGERVAIALTGTDPDAGEGLALRCTPTLGNSTFGKLSGSGCNVTYVANNVNGT
                     DTYPFTVDDGFSGNDQGTLTINVVDPVLPGCTSADSTNARYICRVYLDLLGRAADPSG
                     KAFWLRKVDGGESRVVIIRKYQTTAEYNRRVVDDVYKTFLQRNPDAGGQTFWAEKIRK
                     GANPDEIRTQVIGSNEYYTKAGASSEGFAAAIYQQITRTPATPQQINTVVSQIKAGKS
                     RTSIAAALLATRAGDTATVQGIYERFLRRTPPAAEITYWVNKLQAGITELKLIEATVS
                     SNEYFNRS"
     gene            2459..2962
                     /locus_tag="VNQ33_03535"
     CDS             2459..2962
                     /locus_tag="VNQ33_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97206.1"
                     /translation="MNMFLWLGVASTVLLIVSLVFDASDGFFDLLDGIEVGGDWLSLP
                     VLAAFFGAFGFGAGAFFGSIGLVAIVPGLVLGFVFAVLAARLIRFVDGGPHDAPETEA
                     SLLGAMGRVVTATSPGSYGEVLLQRANGPTKVACRADQVIPLGTEVVVVDVASSTLVT
                     VIPFPTE"
     gene            2970..>4177
                     /locus_tag="VNQ33_03540"
     CDS             2970..>4177
                     /locus_tag="VNQ33_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWJ97207.1"
                     /translation="MSPILIALAGLAVLGVAVVMFVVTRIRVAGPNEAFIITGRKGKG
                     SGDTSGQKVVMGASVFVVPFVQKLGAVDLSSRQIGVSVPAAVSSNGIRCVLEGVAVVK
                     VGGTEELIRAAAQRFLGQQQEIDVFTREVLAGSLRAIVGRLSVEEIIRDRAAFASAVA
                     EEAENSLTGQGLVLDTFQLQDIQAEGDYLVDLGRPEAARAAMEAAVAEANARREAEQA
                     RIRAEEEIAMANRQLSLKQAEIKAQTDAAAAEAAASGPLAQAARDQDVIAAQEQVAAR
                     RADLKERELDTEVRKPADAQRYAVEQEAASHKAQEIARAEAAAEQVRLSGAAEREKRT
                     ALADAVKAEGVAEAAAVLAKGQAQAEAMEKKADAYKHYGEAAVLDIVAEALPKVVAEA
                     ARPMSQIDKVT"
BASE COUNT          548 a         1401 c         1572 g          656 t
ORIGIN      
        1 ccgatggagg acggcccgac gggggtcggc ccgacggcgg ccgcgggtcc gggcgagcgg
       61 cgggggcgcc ggcctccggg cggcggggcg gccgggccgg gccggagcct gcgggacggc
      121 ccgacgatgg cgatccgccc ggacggggcg gccggcgtgg cccgggaggg cgctgggcgt
      181 cggacatcga ggtggtctcc agggtgggga gccggtcggg cggtgcgggt ggacccggac
      241 tcggggaccc gaccagcgta cggaacggcg gatggcggtc ccggtcatgg tgccatcgac
      301 gaacccgttg ccgccggtgg gaggcgccac cagccgctgc catgagatcg gtccggggcc
      361 ggacgcgaac cgcccgcccg gcggcggacg cggggcgggc ggttctgggg gctcggggcc
      421 gctacgagcg gttgaagtac tcgttcgagg acacggtggc ctcgatgagc ttcagctcgg
      481 tgatgccggc ctgcagcttg ttcacccagt aggtgatctc ggctgccggc ggggtgcggc
      541 gcaggaaccg ctcgtagatg ccctggaccg tggcggtgtc gccggctcgg gtggcgagca
      601 gcgccgcggc gatcgaggtg cggctcttgc ccgccttgat ctgggacacg accgtgttga
      661 tctgctgcgg agtcgccgga gtccgggtga tctgctggta gatcgccgcg gcgaagccct
      721 cggaggaggc tcccgccttc gtgtagtact cgttcgaccc gatcacctgg gtgcggatct
      781 cgtcggggtt cgcccccttg cggatcttct cggcccagaa cgtctggccg cccgcgtcgg
      841 ggttccgctg gaggaacgtc ttgtagacgt cgtccacgac ccggcggttg tactccgcgg
      901 tggtctggta cttgcggatg atcacgacgc ggctctcgcc cccgtccacc ttgcgcagcc
      961 agaaggcctt gccgctcggg tccgcggccc ggccgaggag atccaggtag acccggcaga
     1021 tgtaccgggc gttggtcgag tcggccgacg tgcagcccgg cagcaccgga tccacgacgt
     1081 tgatggtgag cgtgccctgg tcgttcccgc tgaagccgtc gtcgacggtg aacgggtagg
     1141 tgtcggtgcc gttcacgttg ttggccacgt aggtgacgtt gcagcccgag ccgctgagct
     1201 tgccgaacgt ggagttgccg agcgtcggcg tgcagcggag ggccaggcct tcgccggcgt
     1261 cggggtcggt gccggtgagg gcgatggcca cccgctcgcc tcgtccgacg gtcacggtct
     1321 ggtcggtggc ggtgggtgct cggttcgtga tgttgaacac cacgttggcg ttggcgctcg
     1381 acgagccgtt gacgtcggtg accttgaagg cgaagccgtc ggtgccagag gtgcgggtgg
     1441 cggcggtgta ggtgcggttg cagcccgagc cggccagcgt gcccttgccc tgggtggtgg
     1501 tggcgggtgc ggtgcaggtg agcgggtctc cgtcaggatc ggtgccggtg agcacgaccg
     1561 gaacgggcgt gcccttgtac gcggtgacga cctgcgggtt ggcgacgggc gggctgttgt
     1621 tcgtgctcag ctgcagctcc cagccgccgt tgaagccacc gctgtcgctg gaggcatcgt
     1681 cgaccacgta cagcttccat gcgccgttcg ggttcttgcc tgcgaaggtc gagaggttga
     1741 gcgccgggaa gttgtcgccc acgtcgggcc aggagtcgcc ctggtgcccg agggcgtcgt
     1801 tgtcggacgg ccggacgcag atgttggctc cgctgggcat ggagttggcg aagtcggggc
     1861 aggcgtcgtc gaaggtgatc gagtccttct gcaggttggt gcagccgtcg aactcgatcg
     1921 ccggggcgcc gttgtccgac atcagggtgg tcttggtgcc gtcgggcccg acgaggagca
     1981 cgtcgaggtc cttggtgcag ccgtggttgg cgccgcgcac cacgaccttc aggctgagca
     2041 ggctggtccc gagccccgac acgttgatgg tggacgggta caggccggcc ttgccggagt
     2101 ccggcgtgat gatggggctg gtgttcttga gcgtggcggt gctcaccgct ccggccgggg
     2161 gtgccccgac cacgctggcg accacccccg ccagcagtgc acctgcgagc gccactgcgc
     2221 ccgtccgccg agtgacgcgt cgcgtccgtc cctgcaacat gcgaaccctc catgggagcc
     2281 gacgaccccc ctggctgccg gctggtggca gtgtgaccgc ggaccctggc ccatgcaagc
     2341 cagacccgag cgttcgctcg gatcgtcccc ctgggcgggt gggtaccagg ccacccgcgg
     2401 gccggatcgg aaactcctag cgacccggag cagcccgttc gctagggtcg gcttgaacat
     2461 gaacatgttc ctgtggctcg gggtggcgag caccgttctg ctcatcgtgt cgttggtctt
     2521 cgatgcctcc gacggcttct tcgacctgct cgacggcatc gaggtgggcg gcgactggct
     2581 gtcgctcccc gtcctggcgg cgttcttcgg agcgttcggc ttcggcgccg gcgccttctt
     2641 cgggtcgatc gggctcgtgg cgatcgtgcc cggtctcgtc ctcggcttcg tgttcgctgt
     2701 tctggccgcc cgcctcatcc gcttcgtcga cgggggcccg cacgacgcgc ccgagaccga
     2761 ggcatcgctg ctcggggcga tgggccgcgt cgtcaccgcc accagccccg gttcctacgg
     2821 cgaggtcctg ctgcagcgag ccaacggtcc caccaaggtc gcctgccgag ccgaccaggt
     2881 catcccgctc ggaaccgagg tggtcgtcgt cgacgtcgcc tcgtccaccc tcgtcaccgt
     2941 catccccttc cccacggagt gaacccctga tgtctcccat cctcatcgcc ctcgccggtc
     3001 tggccgtgct cggcgtggcc gtcgtcatgt tcgtcgtgac gcgcatccgg gtggccggcc
     3061 cgaacgaggc gttcatcatc accggccgca agggcaaggg cagcggcgac acctcgggcc
     3121 agaaggtggt catgggggcg agcgtcttcg tggtgccctt cgtccagaag ctcggcgccg
     3181 tcgacctcag cagccgccag atcggggtca gcgtcccggc cgccgtctcg tcgaacggga
     3241 tccgctgcgt gctcgagggg gtggccgtcg tcaaggtcgg cgggaccgag gagctgatcc
     3301 gggccgcggc ccagcggttc ctcggccagc agcaggagat cgacgtgttc acccgcgagg
     3361 tgctggccgg ctcgctgcgc gccatcgtcg ggcgcctgtc ggtcgaggag atcatccgcg
     3421 accgcgccgc cttcgcctcg gccgtcgccg aagaggccga aaacagcctc accggccagg
     3481 gcctggtgct cgacaccttc cagctccaag acatccaggc cgagggcgac tacctcgtcg
     3541 acctcggccg gcccgaggct gcccgcgccg cgatggaggc cgccgtggcc gaggccaacg
     3601 cccgccgtga ggccgagcag gcccgcatcc gcgccgagga ggagatcgcg atggccaacc
     3661 ggcagctgtc gctgaagcag gccgagatca aggcccagac cgatgccgcg gcagccgagg
     3721 cggccgcctc cggacccctg gcccaggccg cacgggacca agacgtcatc gccgcccagg
     3781 agcaggtggc cgcccgccga gccgacctga aagagcgcga gctcgacacc gaggtccgca
     3841 agccggccga tgcccagcgc tacgccgtcg agcaggaggc ggcctcgcac aaggcccagg
     3901 agatcgcccg ggcggaggcc gccgccgaac aggtccggct gtcgggcgcc gccgaacggg
     3961 agaagcgcac ggcgctcgcc gacgcggtca aagccgaggg tgtggccgag gccgcggccg
     4021 tgctggccaa gggccaagcg caggccgaag cgatggagaa gaaggccgac gcgtacaagc
     4081 actacggcga ggccgcggtg ctcgacatcg tcgccgaggc cctgccgaag gtcgttgccg
     4141 aagccgcccg gccgatgtcg cagatcgaca aggtcac
//
LOCUS       DATMFS010000193         4341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53329, whole genome shotgun sequence.
ACCESSION   DATMFS010000193 DATMFS010000000
VERSION     DATMFS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53329"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VNQ33_03545"
     CDS             complement(<1..1443)
                     /locus_tag="VNQ33_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWJ97208.1"
                     /translation="MVLALVAAGCSSESSDGKDGGPQQPAVDIEAHGGTGQVWLKTEP
                     GAELELQDADGKVVPTQVVDDTAQVTKVDTRTADDNGALVFRYVPVGKGYVVKQVDGP
                     GESKPVDVTGVDDHPAQTFYDDQKIEEGYGYLTTRDGTKLAINVTLPGPVEDGPYPTV
                     VEYSGYDPANPKPSLVTISKTLAGGQGFATVGVNVRGSGCSGGSFQLWEDAQAVDGYD
                     VVETIAAQPWVKGHKVGMVGISYPASAMLYTAATNPPHLAAIAPMAGYDDGFRALLWP
                     GGVQNKGFARSWVEERDKNAKPLEADWTKKQVKEGDATCKENMELRGQNVALSELIDA
                     MPYFPTQNDLGDSFAPSTFVNKIDVPTFLVASWQDEQVGGHAPTMIPNLEHVPHLYAT
                     LTNGLHTEGLANPTVLQRWLEFLQLYVAEEVPDTTGMAPLYHAVPAEIIGDADAASTL
                     PIPPEHFNQSTTYEDALAEYEDQPPVRVLFD"
     gene            1663..2328
                     /locus_tag="VNQ33_03550"
     CDS             1663..2328
                     /locus_tag="VNQ33_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HWJ97209.1"
                     /translation="MVYARWLLGEIYGLVQINGFWMTWNLLLAFIPAALAVPLLWRPH
                     ARTAAWWVGIGVFALFLPNAPYVLTDLIHLRWDAARAASDGVLVFGVLPIYGSFVLVG
                     MSAYLFCVEGIVREVRSVRPGASRSLIELPVHALCSLGIVLGRIARLNSWDTITEPVG
                     TLESVFATLTWRGAPAAFIVILVAVWASFTVLRTLVRAAADWGSGWANRFGLAGSAPR
                     PAS"
     gene            complement(2344..3330)
                     /locus_tag="VNQ33_03555"
     CDS             complement(2344..3330)
                     /locus_tag="VNQ33_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWJ97210.1"
                     /translation="MGTDGFTLAGTEVAIPALGVGTWAWGDAKTWGYGTYDAALTGDT
                     IRDAWDASIDGGATFFDTAEVYGNGESERIIGRLLAEDPVRAAKVQLATKFMPVPWKV
                     NVRSALLSSLRASLDRLGLERVDLYQIHGPVSLRGHGALAEALAAAHQEGLVRSVGVS
                     NYSVSETKKFAAALRERGLPLASNQIEFSLLRTSPERSGLLATCRELGVVPLAYSPIG
                     QGRLTGKYTAENPPPSTRNFSAHPMEEVDAVVAELRRVGEANGGKTPSQVALNWIIAK
                     GAVPIPGAKNREQAEQNAGALGWSMGDDDVAALDAVALEGVRSLSNRLWQHG"
     gene            3394..4275
                     /locus_tag="VNQ33_03560"
     CDS             3394..4275
                     /locus_tag="VNQ33_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97211.1"
                     /translation="MTPTQRILLAAAVAAFATAAAACGTSTGPEAIDDPRPTTTAASP
                     PTTDPAGGDEIVWQEFTGGGFTPAAYALGGVPDVTIYADGRILRSVDADADTGTLRPV
                     DLEEAQIPAGELAAFLDAAADSALFEPGTDFGQPGVTDLPSTSVTLRTGDTPQSVDVY
                     ALDLTIDPSSRDVTPEQAERRDQLKDLLAQARSLAKGATPYVPGRVRASLVTSGSQID
                     AGAAPRDWPGPPLSAFPEPAADGSDTSCLVVEGPDAATVIEAAADNQSAFWRIGGEVH
                     QIVTAPLVPGEEGCPPR"
BASE COUNT          582 a         1603 c         1483 g          673 t
ORIGIN      
        1 atcgaacagg acgcgcaccg gcggctggtc ctcgtactcg gcgagggcgt cctcgtaggt
       61 ggtgctctgg ttgaagtgct ccggcgggat cggcagcgtc gacgctgcgt ccgcgtcgcc
      121 gatgatctcg gcaggaacgg cgtggtacag cggggccatg cccgtggtgt cggggacctc
      181 ctcggccacg tacagctgca ggaactccag ccaccgctgc aggaccgtcg ggttcgccag
      241 gccctcggtg tggaggccgt tggtcagcgt ggcgtacagg tgcgggacgt gctcgaggtt
      301 cgggatcatc gtcggggcgt ggccgccgac ctgctcgtcc tgccacgacg ccaccaggaa
      361 cgtgggcacg tcgatcttgt tcacgaacgt cgacggcgcg aaggagtcgc ccaggtcgtt
      421 ctgcgtcggg aagtacggca tggcatcgat cagctcggac agggccacgt tctggccgcg
      481 cagctccatg ttctccttgc aggtcgcatc gccctccttg acctgcttct tggtccaatc
      541 agcctcgagc ggcttggcgt tcttgtcgcg ctcttcgacc cagctgcgag cgaagccctt
      601 gttctgcacg ccgccgggcc agagcagggc acggaagccg tcgtcgtagc cggccatcgg
      661 ggcgatggcg gcgaggtgcg gcgggttggt ggccgccgtg tagagcatcg ccgaggccgg
      721 ataggagatg ccgaccatcc cgaccttgtg gcccttgacc cacggttggg cggcgatggt
      781 ctcgacgacg tcgtagccat cgacggcctg ggcgtcttcc cacagctgga aggagccacc
      841 cgaacagccc gaaccgcgga cgttgacgcc gacggtcgcg aagccctgcc cgccggcgag
      901 cgtcttggag atggtcacca gcgacggctt cgggttggcc gggtcgtagc cggagtactc
      961 gaccacggtc gggtacggcc cgtcctcgac ggggccgggg agggtgacgt tgatggcgag
     1021 cttcgtccca tcccgcgtgg tcaagtagcc gtagccctcc tcgatcttct ggtcgtcgta
     1081 gaacgtctgg gcggggtggt cgtcgacgcc ggtcacgtcg accggcttcg actcgccggg
     1141 cccatcgacc tgcttgacca cgtagccctt gcccaccggc acgtaccgga acacgagcgc
     1201 gccgttgtcg tcggcggtgc gcgtgtcgac cttggtcacc tgcgccgtgt cgtccaccac
     1261 ctgggtcggc acgaccttgc cgtcggcgtc ctggagctcg agctccgcgc cgggctcggt
     1321 cttgagccac acctgtccgg tgcccccgtg cgcctcgatg tcgaccgcgg gctgctgcgg
     1381 cccaccgtcc ttgccatccg aactttcgga gctgcaaccc gccgccacca gcgccagcac
     1441 catgaccgca gccgcagctg cccgtcgaac ctgcacacca acccccagaa acggcttgct
     1501 gagcgcccga ccctacgggc cctcctccgc acgggacgca caccagtgtc agcgagctcg
     1561 ccccggggga cgcccatgcg gagatgtgat ggtagcaaca accgaccatc ggtcggtttc
     1621 agcccgaccg tcgcgtgcga cgccggtcca ccagactgga gcatggtcta cgcgcgctgg
     1681 ctcttgggcg agatctacgg cctcgtccag atcaacggct tctggatgac gtggaacctc
     1741 ctcctcgcgt tcatccctgc ggcgctggcc gtgcccctgc tgtggcgtcc gcacgcccgc
     1801 acagcggcct ggtgggtggg catcggcgtg ttcgcgctgt tcctcccgaa cgcgccgtac
     1861 gtcctgaccg acctcatcca cctccggtgg gacgcggccc gcgcggcgag cgacggtgtc
     1921 ctggtgttcg gggtcctgcc gatctacggg tcgttcgtcc tcgtcggcat gagcgcctac
     1981 ctcttctgcg tcgagggcat cgtgcgggag gtgcgctcgg tgcgacccgg cgcctctcgg
     2041 tccctgatcg aactgccggt ccacgccctc tgctcgctcg gcatcgtctt gggccgcatc
     2101 gcccggctca acagctggga caccatcacc gaaccggtcg gcacactcga gtccgtgttc
     2161 gccacgctca cctggcgcgg cgcacccgcc gcgttcatcg tgatcctcgt cgcggtctgg
     2221 gcctccttca ccgtgctgcg cacgctcgtc cgagcagcgg cggactgggg ctcgggctgg
     2281 gcgaaccggt tcggcctcgc gggcagcgcg ccccgccccg cctcctgatc ggccgaaccg
     2341 ggctcagccg tgctgccaga ggcggttcga caggctgcgc acgccttcga gcgccaccgc
     2401 gtcgagcgcg gcaacgtcgt cgtcgcccat cgaccagccc agcgcacccg cgttctgctc
     2461 ggcctgctcg cggttcttgg cgccggggat cggcaccgct cccttggcga tgatccagtt
     2521 gagcgcgacc tggctcgggg tcttgccgcc gttcgcctcg ccgacgcgcc ggagctcggc
     2581 gaccacggcg tcgacctcct ccatcgggtg ggccgagaag ttccgggtcg acggaggcgg
     2641 gttctccgcc gtgtacttcc cggtcaggcg gccctgcccg atcggcgagt aggccagcgg
     2701 cacgacgccg agctcgcggc acgtggcgag gagcccgctc cgctcgggcg acgtgcgcag
     2761 gagcgagaac tcgatctggt tcgacgccag cggcagaccc cgctcgcgca gggcggctgc
     2821 gaacttcttc gtctccgaca ccgagtagtt cgacacgccc accgatcgga cgaggccctc
     2881 ctggtgcgcg gccgccagcg cttcggcgag ggcgccgtgg cctcggaggc tgaccgggcc
     2941 gtggatctgg tagaggtcga cgcgctcgag accgagccga tcgagcgacg cccgcagcga
     3001 cgacaagagc gcggagcgca cgttgacctt ccacggcacc ggcatgaact tcgtggcgag
     3061 ctgcaccttg gccgcccgta ccggatcctc ggccaggagg cgcccgatga tgcgctcgct
     3121 ctcgccgttc ccgtagacct cggcggtgtc gaagaacgtg gcacctccgt cgatcgacgc
     3181 gtcccacgcg tcacggatcg tgtcaccggt gagggcggcg tcgtaggtgc cgtagcccca
     3241 ggtcttggcg tcaccccacg cccacgtccc cacgcccagc gcggggatcg ccacctcggt
     3301 gccggccagc gtgaacccat cggtgcccat cgccgcaacg tacctccggg ggaacgaacc
     3361 tggagcgtcc gtcgtcggag ggacgtgcac cccatgacgc caacccagcg aatcctcctg
     3421 gccgccgcgg tggcggcctt cgccaccgcg gcggcggcct gcgggacgag caccggcccg
     3481 gaggcgatcg acgacccgcg gccgaccacc accgcggcat cgccgccgac gaccgatccg
     3541 gccggcggtg acgagatcgt ctggcaggag ttcaccggtg gcgggttcac gcccgcggcg
     3601 tacgcgctcg gcggcgtccc cgatgtgacc atctacgccg acggccgcat cctgcgctcg
     3661 gtcgacgccg atgccgacac cggcaccctc cgcccggtcg acctcgagga agcgcagatc
     3721 cccgccgggg agctggcggc gttcctcgac gccgccgccg actcggccct gttcgagccg
     3781 ggtaccgact tcggccagcc cggcgtcacc gacctgccgt ccaccagcgt caccctgcgc
     3841 accggcgaca cgccgcagtc cgtcgacgtc tacgccctgg acctcaccat cgacccgtcg
     3901 tcccgcgacg tgacaccaga gcaggcggag cggcgggatc agctcaagga cctcctggcc
     3961 caggcccgct cgctggcaaa gggggccacg ccctacgtac ccgggcgcgt gcgggcgagc
     4021 ctcgtcacgt ccggcagcca gatcgacgcc ggtgcggcgc cgcgggactg gcccggcccg
     4081 ccgctgtccg ccttccccga gccagcggcc gacgggtccg acacctcgtg cctggtggtc
     4141 gaaggtcccg acgccgccac cgtgatcgag gcggcggccg acaaccagag cgcgttctgg
     4201 cgcatcggcg gcgaggtcca ccagatcgtc accgcccccc tcgtgcccgg cgaagagggc
     4261 tgtccacccc gctgaccggg gcgccggacc ggcccgggtc aggcggtcgg ggcgtcggcg
     4321 tcgggatgct cggcgaggag g
//
LOCUS       DATMFS010000194         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_101110, whole genome shotgun sequence.
ACCESSION   DATMFS010000194 DATMFS010000000
VERSION     DATMFS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101110"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VNQ33_03565"
     CDS             <1..841
                     /locus_tag="VNQ33_03565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440870.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWJ97212.1"
                     /translation="IAVRLRKRVRDEVGLAISVGIARTKFLAKVASAVGKPDGLLEVP
                     AGTELDFLHPLPVERLWGVGPVTARKLHERGITTVSQVAELPEPSLVGLLGAGSGRHL
                     FALAHNRDPRRVDTGRRRRSIGSQQALGRGPRSVDDLDAVLIGLVDRVSRRMRTAERV
                     GRTVVLRLRFGDFTRATRSHTLAEATDETLVLLQAVRALLRAEVPRLRTDGVTLLGLS
                     VTNLQDAKAVQLALPFDGKDRRRLDAAVDQVRTKFGTKSIGRTTLVTRPLREEAPRLP
                     GHD"
     gene            890..1213
                     /locus_tag="VNQ33_03570"
     CDS             890..1213
                     /locus_tag="VNQ33_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97213.1"
                     /translation="MESTSLQFAATVRTLGAAARERGLVVPGFRSPPRRPGAERTLRW
                     AADGTATVAVAVKGRPLQAVVADLIEGVVLVNQLDGIEATRVRTAMWEAVVAAHSDAA
                     WRGAA"
     gene            1232..1317
                     /locus_tag="VNQ33_03575"
     tRNA            1232..1317
                     /locus_tag="VNQ33_03575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Leu,seq:taa)
     gene            1380..1934
                     /locus_tag="VNQ33_03580"
     CDS             1380..1934
                     /locus_tag="VNQ33_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97214.1"
                     /translation="MATTTVFVDDAVRGRLPEVCAKTGVPATRHLTIHQDLTGGLGAA
                     WVLVFFGPLGWLVLALVAVSRRRSTLTVRIPYADRELDRYVRTARRTFHLAIATGVLL
                     LAVFGAAFGLALSDPSPLVLAGLAVLVLGGVAVLVNGMIARRMLVGVGLDASGRWVLL
                     SRVHPAFAAACHAVPVSTPGRLPA"
     gene            2003..2764
                     /locus_tag="VNQ33_03585"
     CDS             2003..2764
                     /locus_tag="VNQ33_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97215.1"
                     /translation="MGRRHLYLHETIDIVGQGQYDYMAHSLKEPTNVMPDMLTLQGTF
                     FVCAMGGGRWPQVVNIWDVGAAGWEGWAANVDRLNLKRRSAFYGDWWDEASQWRTGGF
                     DRLCGGVPGCPTTTELAERGVKGTLFVHQCLQVRPGSGLEYLAAVVEEQVPLWQEHGH
                     EPTGIYEVLGNQHEVVVVWATSIAAQTRLRAARDAARGLSDEVPGDDRLVDWEERSAE
                     YVTGGDTHLMTPQPGTVYGPESWDEASLEDWLPPA"
     gene            3061..>3387
                     /locus_tag="VNQ33_03590"
     CDS             3061..>3387
                     /locus_tag="VNQ33_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97216.1"
                     /translation="MPFLRRPLVVGALLVVLAVTGCSGAKGGAVSSGGSSSTVPTTEA
                     TTTTAEATTTTAAPTTTTEATTTTTTAPVDPTSVAEPAGEPLRSGSVGSRTKAVQQAL
                     ADQHYDP"
BASE COUNT          400 a         1317 c         1202 g          468 t
ORIGIN      
        1 catcgcggtg cgcctccgga agcgggtgcg cgacgaggtc gggctcgcca tctcggtggg
       61 catcgcccgc accaagttcc tcgccaaggt ggccagcgcg gtcggcaagc ccgacgggtt
      121 gctcgaggtg ccggcgggga cggagctgga cttcttgcac ccgctgccgg tcgagcggct
      181 ctggggcgtc ggcccggtca ccgcccgcaa gctgcacgag cgcggcatca ccaccgtcag
      241 ccaggtggcc gagctgccgg agccgtcgct cgtcggcctg ctcggcgcgg gctcgggccg
      301 ccacctgttc gccctcgccc acaaccgcga cccgcggcgg gtcgacaccg gccgccgtcg
      361 ccggtcgatc ggctcccagc aggcgctggg ccggggtccg cgttcggtcg acgacctcga
      421 cgccgtgctg atcggcctgg tcgaccgcgt gtcccgccgc atgcgcacgg ccgagcgggt
      481 cgggcgcacg gtcgtgctgc gcctccgctt cggcgacttc acccgcgcca cccggtcgca
      541 cacgctcgcc gaggccaccg acgagaccct cgtcctgctc caggcggtgc gagccctgct
      601 gcgggccgag gtcccgcgcc tgcgcaccga cggcgtcacc ctgctcggcc tgtcggtcac
      661 caacctccag gacgccaagg cggtgcagct ggcgctgccg ttcgacggca aggaccgccg
      721 ccggctcgat gcggccgtcg accaggtccg caccaagttc ggcacgaagt ccatcggccg
      781 caccacgctg gtcacgcgcc cgctccgaga agaggcgccc cgcctccccg gccacgactg
      841 agggcacagg accttcgctg tcagaccccc tggggacgat ggctgggtca tggaaagcac
      901 gtccctccag ttcgccgcca ccgtccgcac cttgggtgcc gccgcccggg agcgcggcct
      961 ggtggtcccc ggcttccgct cgcctccccg ccggcccggt gcggagcgca cgctgcgctg
     1021 ggctgccgac ggcacggcca ccgtcgccgt ggcggtcaag ggccggcccc tccaggccgt
     1081 ggtcgccgac ctcatcgagg gcgtcgtcct ggtcaaccag ctcgacggca tcgaggccac
     1141 ccgcgtccgc accgccatgt gggaggccgt cgtcgccgcc cactccgacg cggcctggcg
     1201 cggcgccgcc tagcgtttcg tcccggtccc ggcccgggtg gcggaactgg cagacgcaga
     1261 gggcttaaac ccctcgggtc cgaaaggacc gtgcgggttc gaaccccgcc ccgggcaccg
     1321 gccgcggagc cgggagggct ggagcgacgg gtgaggcgtg gagcggtagc gtccgggcca
     1381 tggcgacgac gacggtgttc gtggacgatg cggtccgggg tcggcttccg gaggtgtgcg
     1441 ccaagaccgg cgtgccggcc acccggcacc tgaccatcca ccaggacctg accggcggtc
     1501 tcggtgccgc atgggtgctc gtgttcttcg gtcccctcgg ctggctggtc ctcgccctcg
     1561 tggccgtgag ccgccgtcgc agcaccctca ccgtgcgcat cccgtacgcg gaccgcgagc
     1621 tcgatcgcta cgtccgcacg gcgcggcgga cgttccacct cgccatcgcc accggcgtgc
     1681 tgctgctggc cgtgttcggc gccgccttcg ggctggcgct gtccgatccg tcgccgctgg
     1741 tgctcgccgg cctcgcggtg ctcgtgctcg gcggcgtcgc cgtgctggtc aacggcatga
     1801 tcgcccgccg gatgctcgtc ggcgtgggcc tcgacgcttc gggccgctgg gttctcctca
     1861 gccgggtgca ccccgccttc gccgccgcct gccatgcggt gcccgtgtcg actccgggcc
     1921 ggcttcccgc ctgacggccg gcccccggcg cgccgggtgg cctcgcccgc cgctcgccgt
     1981 cgctggccta cggtctggcg ccatgggccg acgccacctg tacctgcacg agaccatcga
     2041 catcgtcggg cagggccagt acgactacat ggcccactcc ctcaaggagc ccaccaacgt
     2101 gatgcccgac atgctcacgc tgcagggcac gttcttcgtg tgtgccatgg gcggcgggcg
     2161 ctggccgcag gtcgtgaaca tctgggacgt cggcgcggcg ggctgggagg gctgggccgc
     2221 caacgtcgac cggttgaacc tgaagcggcg cagcgcgttc tacggcgact ggtgggacga
     2281 ggcgtcgcag tggcgcaccg gagggttcga ccgcctctgc ggcggggtcc ccggctgccc
     2341 gacgaccacc gagctggccg agcggggcgt gaagggcacc ctcttcgtgc accagtgcct
     2401 gcaggtccgg cccggttcgg gcctcgagta cctggcggcc gtcgtggagg agcaggtgcc
     2461 cctctggcag gagcacggcc acgagcccac cgggatctac gaggtcctcg gcaaccagca
     2521 cgaggtcgtg gtggtgtggg ccacgtcgat cgcggcccag acccggctcc gggccgcgcg
     2581 cgacgctgcc cggggcctga gcgacgaggt ccccggcgac gaccgcctgg tggactggga
     2641 ggagcggtcc gccgagtacg tcaccggcgg cgacacccac ctcatgacgc cccagccggg
     2701 cacggtctac ggacccgagt cgtgggacga ggcgagcctc gaggattggc tccccccggc
     2761 gtagctcggg caggtcccgt ggcctggccg ttcccgggcg cgccgatgcc gccgtcgcct
     2821 cccccttcct gctcggtcgg gccccgaacg cggacgagac caaggcctgg gtcgaccggg
     2881 ccctcgccgg caccgcccgc accacgctct cctcgcgggg atctggcgcc gagggcgggc
     2941 gcggcccgca tcggcggctg gccctcgacg ctccccacgg ggcggtgacc gccgttcacg
     3001 tgaccgctga cctgcggtgc tgcctgtctt cgcgccggca cgttctgcca gacttccccg
     3061 atgccattcc tgcgccgacc cctcgtcgtt ggcgcgctgc tggtcgtgct cgccgtgacc
     3121 ggctgctcgg gtgccaaggg cggtgccgtc agctcgggcg ggtcgtcgtc gacggtcccc
     3181 accactgagg ccaccacgac gacggccgag gccaccacca cgaccgcggc gcccacgacc
     3241 accaccgagg ccaccaccac gaccaccacg gccccggtcg atcccaccag cgtcgccgag
     3301 ccagccggtg aacccctcag gtcgggctcg gtcggcagcc gcaccaaggc cgtccagcag
     3361 gcgttggcgg accagcacta cgacccg
//
LOCUS       DATMFS010000195         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_8111, whole genome shotgun sequence.
ACCESSION   DATMFS010000195 DATMFS010000000
VERSION     DATMFS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8111"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VNQ33_03595"
     CDS             complement(<1..669)
                     /locus_tag="VNQ33_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWJ97217.1"
                     /translation="MSDRLDRPDRLGIVDWGIGGLGLLGHLDRARPGLDVVYWSDTGS
                     TPYGLVPTAALAARLRSVAGELAGRGCTEVVLACNAASTVVDRLAGAPIPVSGIIAAG
                     VAAVPARLDGVVGVVGGRRTIRSGRYRRVLTRPGRRVVSRVAQPLSAHIEAGRIGTTA
                     FEQDLRRIVAPLRGADAVILACTHYPAASDAFAAELPGTLLIDPAAQMAGDLIARLVP
                     GTGER"
     gene            complement(680..2638)
                     /locus_tag="VNQ33_03600"
     CDS             complement(680..2638)
                     /locus_tag="VNQ33_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ97218.1"
                     /translation="MEAVGTDAGLLSPTLLAQLAAAVRGEVSEGLLTSLAGLLPSIDQ
                     ITLKAVPGTPYSRQKVYESDDLEVMVANWEADFRCAPHDHGGASGFVALLRGSFNERR
                     FAWDGPSLVVTGEAGRAEAEWLAFGPEVIHDMVAPLGGLTLHAYSPPPQRMNVYDLDR
                     EEVLDLVGDFGAWIPDEDVPRTGFAEATEADADHEIVTGSAEAAPAEVIGKPVIWVAH
                     TIQYRGGSAAFGVAADTMVRELAVAQPDADVQLARLDGKADFVAEMGRLAAEGRQIRE
                     LHFIGHSGMYGPMFKSTEWPEQFSPHEWREMQIPFAADGRAFFHACRTARWFAPFFAR
                     TFDVPCSGNHGYTTVSARKDRFKWAGRNPEERKDLYLVATPGKKSHGVAGSVKKYTGA
                     PVEPMTEYLPQDPDGVTSYDKVAERYDAAYVDITVREAEWAWITEHAERARRERGRPF
                     RIAEIGCGNGALLRALDDRGDVEAGFGFDDSVAMVGRAQARSEDHERLQFAPIDGPLI
                     DLPDDSVDVALSFLSFRYLDWDPIMEEVRRVLAPGGRLWVVDMVAQPVRVRELPLLAT
                     STLAHLRMPKRHPQFDQDLHALTSHPDWLTMLEYNPIRAEHEYRWFLESRFPAGKLEV
                     LTVTTTQRVVAFDSGPLEKGVTEPLSYP"
     gene            complement(2695..>3961)
                     /locus_tag="VNQ33_03605"
     CDS             complement(2695..>3961)
                     /locus_tag="VNQ33_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03767 family metallophosphoesterase"
                     /protein_id="HWJ97219.1"
                     /translation="CAQTGDNTDNRTAGEVAWWLGVLAGEVVTPNSGAEGTYEGVQRS
                     GWRGAWHPDDPSHDIYGRAGFPCLPGFLDAAVSPFQATGLEVPWLAVFGNHDVLYQGT
                     FGERATHLGDLLASAGTKPRGAFGLVRSNVHARLAGTGADRWEGWGKGPGVQQVTPDP
                     ASRRSVLLDEYLQKLVDAGGHGYTQHHVEDRQSWWSQPAGDGVQVIGLDTCSHRQGSG
                     GSLGPKQTAWLEQELQQCHRRYRNPSGTWVQSGGSDRLVVLLSHHNSWTMDNRHEDDV
                     DPGPRTLGSDLVATLARFPNVVLWVNGHSHEHKILVHRGSAPGSGCWEVDTASGIDFS
                     QQGRTFELVDNGDGTISIFVTVLDHAAPPAVEHRTDDGWTTAELASISRELAANDDRW
                     IDPFALLGGPEDRNVELVVAAPFPLGEAG"
BASE COUNT          540 a         1405 c         1476 g          540 t
ORIGIN      
        1 ccgctccccc gtccccggca cgaggcgggc gatgaggtca ccggccatct gggcggctgg
       61 atcgatcagc agcgttcccg gcagctctgc cgcgaaggca tcgctcgccg ccggatagtg
      121 cgtgcaggcg aggatcacgg cgtcggcgcc tcggagcggg gcgacgatgc gacgcaggtc
      181 ctgctcgaag gcggtggtgc cgatgcgtcc ggcctcgatg tgggccgaca gcggctgggc
      241 gacgcgggac acgacgcggc ggccgggtcg ggtgagcacc cggcggtagc gaccgctgcg
      301 gatcgtgcgg cggccgccga ccacgcccac gacgccgtcg agtcgggccg gcaccgcggc
      361 cacgccggcg gcgatgatcc ccgagaccgg gatcggtgcg cccgcgaggc ggtcgacgac
      421 ggtgctcgcc gcgttgcagg ccagcaccac ctcggtgcag ccccgaccgg ccagctcccc
      481 cgccacggac cggaggcggg cggcgagggc agccgtgggc accagtccgt acggggtgga
      541 cccggtgtcg gaccagtaga cgacgtcgag gcccggccgg gcgcgatcga ggtgaccgag
      601 gaggccgagc ccaccgatgc cccagtcgac gatcccgagg cggtccggtc ggtcgagccg
      661 gtcgctcacc gcagcgccgt cacgggtacg acagcggctc ggtgacgccc ttctccagcg
      721 gaccggagtc gaacgccacc actcgctggg tcgtcgtcac cgtcaggacc tcgagcttgc
      781 cggccgggaa ccggctctcc aggaaccagc ggtactcgtg ctcggcccgg atcgggttgt
      841 actcgagcat cgtcagccag tcggggtgcg acgtgagggc gtgcaggtcc tggtcgaact
      901 gcgggtggcg cttcggcatg cgcaggtgcg cgagcgtcga cgtggcgagc aacggcagct
      961 cgcggacccg caccggctgg gcgaccatgt cgaccaccca gaggcggcca ccaggggcca
     1021 gcacccgccg gacctcctcc atgatcggat cccagtcgag gtaccggaac gacaggaacg
     1081 acagggccac gtcgaccgag tcgtcgggca ggtcgatcag cggaccgtcg atcggggcga
     1141 actgcagccg ctcgtggtcc tcgctgcggg cctgggcccg gccgaccatg gcgacggagt
     1201 cgtcgaagcc gaagccggcc tcgacgtcgc cgcggtcgtc cagcgcccgc agcagcgcgc
     1261 cgttgccgca gccgatctcg gcgatgcgga acggccgacc ccgttcgcgc cgggcgcgct
     1321 cggcgtgctc ggtgatccac gcccactcgg cctcgcggac ggtgatgtcg acgtaggcgg
     1381 cgtcgtaccg ctcggcgacc ttgtcgtagg aggtgacgcc gtcggggtcc tgcgggaggt
     1441 actcggtcat gggctcgacc ggcgcccccg tgtacttctt gaccgagccg gcgacgccgt
     1501 gggacttctt gcccggcgtg gccaccaggt acaggtcctt gcgctcctcg gggttccggc
     1561 cggcccactt gaagcggtcc ttgcgggccg agaccgtggt gtagccgtgg ttgccggagc
     1621 agggcacatc gaacgtgcgg gcgaagaacg gggcgaacca gcgggcggtc cgacaggcgt
     1681 ggaagaacgc tcggccgtcg gccgcgaacg ggatctgcat ctcccgccac tcgtggggcg
     1741 agaactgctc gggccactcg gtcgacttga acatcggccc gtacatgccg gagtggccga
     1801 tgaagtggag ctcgcggatc tggcggccct cagcggccag ccggcccatc tccgccacga
     1861 agtcggcctt gccgtcgagc cgggcgagct ggacgtcggc atcgggctgg gcgacggcca
     1921 gctcgcggac catggtgtcg gcagccacgc cgaacgctgc tgagccgccc cggtactgga
     1981 tcgtgtgggc cacccagatg accggcttgc cgatgacctc ggccggcgcg gcctcggcgg
     2041 acccggtgac gatctcgtgg tcggcgtcgg cctcggtcgc ctcggcgaac cccgtgcggg
     2101 gcacgtcctc gtcggggatc caggcgccga agtcgcccac gaggtccagg acctcctcgc
     2161 ggtcgaggtc gtagacgttc atgcgctgcg gcggcggcga gtaggcgtgg agcgtcagcc
     2221 cacccagcgg ggcgaccatg tcgtggatca cctcgggccc gaaggccagc cactcggcct
     2281 cggcccggcc ggcctcgccg gtcaccacga ggctcggtcc gtcccaggcg aaccggcgct
     2341 cgttgaacga gccccggagc agggcgacga acccgctcgc cccgccgtgg tcgtgcggcg
     2401 cgcagcggaa gtccgcctcc cagttggcga ccatcacctc gaggtcgtcg gactcgtaga
     2461 ccttctgccg ggagtagggc gtacccggca cggccttcag cgtgatctgg tcgatcgacg
     2521 ggaggagccc ggcgagcgag gtcagcagac cctcggatac ctcgccgcgc acggccgcgg
     2581 cgagctgtgc cagcagcgtc ggggacagca gccccgcatc cgtcccaacc gcttccatgc
     2641 cctgcctccc gccctgggag tcaaccacac cgcaccgccg ggcggggatg aaggtcagcc
     2701 ggcctcgccg agcgggaacg gagcggccac gaccagctcg acgttgcggt cctcgggccc
     2761 gccgaggagg gcgaacgggt cgatccagcg gtcgtcgttc gccgccagct cccggctgat
     2821 cgaggccagc tccgcggtgg tccacccatc gtcggtgcgg tgctcgaccg ccggcggtgc
     2881 cgcgtggtcc agcacggtca cgaagatcga gatcgtcccg tcgccgttgt cgaccagctc
     2941 gaacgtgcgg ccctgctggc tgaagtcgat gccgctggcc gtgtcgacct cccagcaccc
     3001 gctgccgggg gcgctcccgc ggtgcacgag gatcttgtgc tcgtggctgt ggccgttgac
     3061 ccagagcacc acgttcggga accgagccag cgtggcgacg aggtcggaac cgagcgtgcg
     3121 gggaccgggg tcgacgtcgt cctcgtggcg gttgtccatc gtccagctgt tgtggtggct
     3181 gagcaggacg acgagccggt cgctgccacc cgactgcacc caggtgccgc tcgggttgcg
     3241 gtaccggcgg tggcactgct gcagctcctg ttcgagccag gccgtctgct tcgggccgag
     3301 cgagccgccc gacccctggc ggtggctgca cgtgtcgagg ccgatgacct ggaccccgtc
     3361 gcccgccggc tgggaccacc acgactgccg gtcctcgacg tggtgctgcg tgtagccgtg
     3421 accaccggcg tcgaccagct tctgcaggta ctcgtcgagc aggaccgagc ggcgggacgc
     3481 ggggtcgggc gtgacctgct ggacgccggg ccccttcccc cagccctccc agcggtcggc
     3541 accggtgccg gccagccgcg cgtgcacgtt ggagcggacg aggccgaagg cgccccgggg
     3601 cttggtgccg gcgctggcga ggaggtcgcc caggtgcgtg gcccgctcac cgaaggtgcc
     3661 ctggtagagc acgtcgtggt tgccgaacac ggcgagccac gggacctcga ggccggtcgc
     3721 ctggaacggg ctcaccgctg cgtcgaggaa gccgggcaag caggggaagc cggcccgtcc
     3781 gtagatgtcg tggctcgggt cgtcggggtg ccaggcgccg cgccacccgg agcgctgcac
     3841 gccctcgtag gtgccctcgg cacccgagtt cggggtgacc acctcaccgg cgagcacgcc
     3901 gagccaccag gcgacctcgc cggccgtgcg gttgtcggtg ttgtcgccgg tctgggcgca
     3961 c
//
LOCUS       DATMFS010000196         4808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_194234, whole genome shotgun sequence.
ACCESSION   DATMFS010000196 DATMFS010000000
VERSION     DATMFS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194234"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VNQ33_03610"
     CDS             complement(<1..827)
                     /locus_tag="VNQ33_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWJ97220.1"
                     /translation="MSKTNPGNFFEDFELGQVIHHATPRTITEGDRAVYGALYPTRFS
                     IPSSAAFAAASGLSPAPVEELIGFHIAFGKTVPDISLNAVANLGYAELRFHRPLVPGD
                     TLRTSSEVIGLKENSNAKTGVVYVRSTAVDQDGDVAIDWARWVMVHKRDVEAPAPEPV
                     VPELAKVVAPSDLVIPAGLDFTSYDFAAAGEPHRFDDYEVGEKIDHVDGVTLSESEHM
                     MATRLWQNTAKVHFNTEARPDGQRLIYGGHIISMARALSFNGLANAQLISAINGGAHT
                     "
     gene            993..>4808
                     /locus_tag="VNQ33_03615"
     CDS             993..>4808
                     /locus_tag="VNQ33_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWJ97221.1"
                     /translation="MGRCGRAISIIAALGIVLSALVLLPRAPASAADADPPPVPDPFQ
                     VEITSAGFQPSTIEVVAGQSVVFTNNSGATRKVLGDDAIFDSGDIPVGGAFTVAIPDS
                     RIVDFDSPGTPTFTGTLQVGANGFTGPADAKLTGYLPTVPAPVEDPTDFAIEPRWGIQ
                     ASLVRLLVVFSPSATVADGNRLLGAIGGRISGRIGTTQILTIDIAKPAAGSFDGLFDA
                     LAELRSDPAVTAAAMDTVGEEQSVPPVPAGATDAGFAWQPYPLTDQTKTGAFGLLQAR
                     FPQAWNVLDAIRAKKTTVDVGIVDSGYDLNHGEFAGAAGKPARIQYVPSCDYGRCSAN
                     PAEKAARSHGTAASSIIAGSTVGANPLANVVATTGATPGMEAQGFVTFVSQNNSVWGL
                     VDEKKPGGKAPDLRAINLSLSTMSFERLPESATTRWALTFLNKTCGPGDNDDASGTLP
                     CTPNTEDGFRKEMAARGAIAGVVAHMAADKNVVIVQSATNFSGVSGNASFCVPFGSGN
                     CEIERIDAQWSAEFAWARLHLQSGQPNNILIVGAIGPNKSRDDYSNTGADVSAAGAGV
                     VAATPSSPARLYQGFGGTSAAAPYVTGLVSLLAQYAPNLTAAQIVNLIRDHAIADTTD
                     GAAPRIDAYATLTAVPGALIDVVDVNDVSADGNRRVIIPQEGSGEPRSEDLRFGPVVD
                     GVLLDNEGDGVIDMRDFRRFRDAFLSGCEAGITASADGCPPAVGSIKLNGDPNNIKFD
                     LNLDVCRVACSISEGIAPRSDPAGASSLSPFDEAAVGVNATGTPTTTNRTTSGMEVLQ
                     MLWGKSRAGGAAADTEGYSADDLRWLLSSGDLEVHPDSLMRLGATSMTIKGQVSTDKG
                     MTWTATGKTRTVTLINVKDGYVPFTVPAELGVEGSLVRVVVSGTTADGKKLVTDKPRV
                     TLHAGQDVRVDPKVISAKLQLTPTNLAKGKKGTATVRLQRDGRPVQKKSGTPSNAAPS
                     AASADGATTAADDGSTSDYAISDAAELFGAPVEFTAEGSGAAPADPSVGTADETIDDR
                     GEADAEIDAGEDAGDAEIGATVTVDGDEIEATVPTKVTGPARLIYTWEQRITAWNRHF
                     EGLVDPDPGLPGLGGTYDYDEGPVAGTLPVVMKRTGTIDLDAPIPTLDETVNSADYSI
                     EQTSPFGTGTITNSIQPLRRETKDYLLDRVHVDRDGTSATIAGLRQVSDIGYRYNCNE
                     ANPSNCSALSHLQFFDSWLSITTRRDPNVYKNGQVSGYRGGDGTSFLHAPDPDADLHL
                     RKDSSGHWIP"
BASE COUNT          766 a         1707 c         1671 g          664 t
ORIGIN      
        1 gtgtgggcgc cgccgttgat ggccgagatc agctgggcgt tggccaggcc gttgaacgac
       61 agggcccggg ccatcgagat gatgtggccg ccgtagatga ggcgctggcc gtcggggcgg
      121 gcctcggtgt tgaagtgcac cttggccgtg ttctgccaga ggcgggtggc catcatgtgc
      181 tcggactcgg agagggtgac gccgtcgacg tggtcgatct tctcgcccac ctcgtagtcg
      241 tcgaagcggt gcggttcgcc ggcggcggcg aagtcgtacg aggtgaagtc gagcccggcc
      301 gggatcacca gatctgacgg ggcgacgacc ttggccagct cgggcacgac cggctcgggg
      361 gccggggcct cgacgtcgcg cttgtggacc atcacccagc gggcccagtc gatggcgacg
      421 tcgccgtctt ggtccacggc ggtggaccgc acgtagacca cgccggtctt ggcgttcgag
      481 ttctccttga ggccgatgac ctccgacgac gtgcggagcg tgtcaccggg gacgagcggc
      541 cggtggaagc gcagctcggc gtagccgagg ttggcgacgg cgttgagcga gatgtcgggg
      601 acggtcttgc cgaaggcgat gtggaagccg atgagctcct cgaccggcgc cggcgacagg
      661 ccgctggcgg cggcgaaggc ggccgacgac gggatcgaga accgggtggg gtacagggcg
      721 ccgtagacgg cccggtcgcc ctcggtgatg gtgcggggcg tggcgtggtg gatcacctgg
      781 cccaactcga agtcctcgaa gaagttgccg gggttggtct tgctcatcgt catcccatcg
      841 ggtcgctcgg aacggtccac gacgctaccg agcgcgcccg ggacggcccg aaccgcggcc
      901 tcctcgcgtg cctacgtagt cggctacgga tgcgccttcc gccccgttca ggtccactgc
      961 aggggcagac ctctcgagtc agtggagtca ccgtgggcag gtgcggccgg gccatctcga
     1021 tcatcgcggc gctgggcatc gtcctcagcg cgctcgtctt gctgccgcgg gcgccggcct
     1081 cggcggccga tgcggacccg ccgccggtcc ccgatccgtt ccaggtcgag atcacgtcgg
     1141 cgggcttcca accctcgacc atcgaggtcg ttgccggcca gagcgtggtc ttcacgaaca
     1201 acagcggtgc gacccgcaag gtcctcggcg acgacgccat cttcgactcg ggcgacatcc
     1261 ccgtcggtgg tgcgttcacg gtcgccatcc ccgacagccg gatcgtcgac ttcgactccc
     1321 cgggaacgcc caccttcacc ggcacgctcc aggtcggcgc caacggcttc accggccccg
     1381 ccgacgcgaa gctcaccggc tacctgccga ccgttccggc cccggtggag gatccgaccg
     1441 acttcgcgat cgagccccgg tggggcatcc aggcgtcgct cgtgcgcctg ctcgtcgtct
     1501 tctcgccgag cgcgaccgtg gccgacggca atcggctcct cggagccatc gggggtcgga
     1561 tctccggccg gatcgggacc acccagatcc tcacgatcga catcgccaag ccggccgccg
     1621 gaagcttcga cggcctgttc gacgccttgg cggagctccg gtcggatccg gcggtgacgg
     1681 cggcggcgat ggacacggtg ggcgaggagc agtcggtgcc ccccgttccc gccggcgcca
     1741 ccgatgccgg cttcgcgtgg cagccctacc cgctcacgga tcagacgaag acgggtgcct
     1801 tcgggctcct ccaagcgcgg ttcccccagg cgtggaacgt cctcgacgcg atccgggcga
     1861 agaagaccac ggtcgacgtc gggatcgtcg actccggata cgacctgaac cacggcgagt
     1921 tcgcaggcgc ggcgggcaag ccggcgcgca tccagtacgt cccgagctgc gactacgggc
     1981 gctgctcggc caacccggcg gagaaggcgg cgcgcagcca cgggacggct gcctcctcga
     2041 tcatcgcggg cagcacggtg ggcgccaatc cgctggcgaa cgtcgtggcc accacgggag
     2101 cgacgccggg gatggaggcc cagggcttcg tcaccttcgt ctcccagaac aactcggtct
     2161 gggggctggt cgacgagaag aagcccgggg gcaaggctcc cgacctccgt gccatcaacc
     2221 tcagcttgag cacgatgtcg ttcgagcggc tgcccgagag cgccaccacg cgctgggcgc
     2281 tcacgttctt gaacaagacg tgcgggcccg gcgacaacga cgatgccagc ggcacgctcc
     2341 cgtgcacgcc gaacaccgag gacggcttcc gcaaggagat ggcggctcgc ggcgcgatcg
     2401 cgggcgtcgt ggcccacatg gcggccgaca agaacgtcgt gatcgtccag tccgcgacca
     2461 acttcagcgg cgtgtccggc aacgcctcgt tctgcgtgcc cttcgggagc gggaactgcg
     2521 agatcgagcg gatcgacgcc cagtggagcg ccgagttcgc ctgggcccgc ctgcacctcc
     2581 aatcggggca gcccaacaac atcctcatcg tcggtgcgat cggaccgaac aagagccgcg
     2641 acgactactc caacaccggc gccgacgtgt cggccgccgg ggccggcgtc gtcgccgcca
     2701 cgcccagctc gcccgcccgc ctgtaccagg gcttcggcgg cacgtccgcg gccgcgccgt
     2761 acgtcacggg tctggtcagc ctcctcgcgc agtacgcccc gaacctcacc gcggcccaga
     2821 tcgtgaacct gatccgggac cacgcgatcg ccgacaccac cgacggcgcc gccccccgca
     2881 tcgacgccta cgccacgctc accgcggtcc cgggcgcgtt gatcgacgtc gtggacgtga
     2941 acgacgtgtc ggccgacggc aaccgccggg tcatcatccc ccaggaggga tccggcgagc
     3001 cccgttccga ggacctccgg ttcggccccg tggtcgatgg cgtgctcttg gacaacgagg
     3061 gtgacggcgt catcgacatg cgcgacttcc ggcgcttccg agatgcgttc ctctcgggct
     3121 gcgaggcggg catcaccgcc tcggcggacg ggtgcccgcc cgcggtgggg agcatcaagc
     3181 tgaacggcga ccccaacaac atcaagttcg acctcaacct ggacgtctgc cgggtcgcct
     3241 gctcgatctc cgagggcatc gccccccgca gcgacccggc cggtgcgagc agcttgagcc
     3301 ccttcgacga agccgcggtc ggcgtcaacg cgacgggcac gcccacgacc accaaccgga
     3361 ccacgtcggg gatggaggtg ctgcagatgc tctggggcaa gagccgcgcc gggggcgcgg
     3421 ccgccgacac ggagggctac tcggccgacg acctccggtg gctgctcagc tccggtgacc
     3481 tcgaggtgca ccccgacagc ctgatgcggc tgggggcgac ctcgatgacg atcaagggcc
     3541 aggtcagcac ggacaagggc atgacctgga cggcgaccgg caagacccga acggtgacgc
     3601 tcatcaacgt gaaggacggg tacgtcccgt tcaccgtccc ggccgagctc ggcgtcgagg
     3661 gctcgctcgt gcgggtcgtg gtgtccggca cgacggcgga cggcaagaag ctggtgaccg
     3721 acaagccgcg cgtcacgttg cacgccggcc aggacgtccg ggtcgacccg aaggtcatct
     3781 cggcgaagct gcagctgacg ccgacgaacc tcgccaaggg caagaagggc acggcgacgg
     3841 tgcggctcca gcgcgacggc cgccccgtcc agaagaagtc gggcacccca tcgaacgctg
     3901 cgccgtccgc cgcctcggcc gatggcgcga cgaccgccgc cgacgacggc tcgacgtcgg
     3961 actacgcgat cagcgatgcg gccgagctgt tcggcgcacc ggtcgagttc acggcagaag
     4021 gatcaggggc cgctcccgcc gacccgtcgg tgggcacggc cgacgagacg atcgacgacc
     4081 gcggcgaggc cgacgccgag atcgacgccg gtgaggacgc gggcgacgcc gagatcggcg
     4141 cgaccgtgac cgtcgacggc gacgagatcg aagcgacggt gccgacgaag gtcacggggc
     4201 cggcccgcct catctacacc tgggagcagc ggatcaccgc gtggaaccgc cacttcgagg
     4261 ggctggtcga cccggatccg ggtctgcccg gcctcggggg cacctacgac tacgacgagg
     4321 ggccggtggc cggcaccctt ccggtcgtca tgaagcgcac cgggaccatc gacctcgatg
     4381 cgccgatccc gacgctcgac gagacggtga actcggcgga ctactcgatc gagcagacgt
     4441 cgccgttcgg gaccgggacc atcaccaaca gcatccagcc gctccgccgc gagacgaagg
     4501 actacctcct ggaccgggtc cacgtggacc gagacggcac ctcggcgacg atcgccggtc
     4561 tgcgccaggt cagcgacatc ggctaccgct acaactgcaa cgaggccaac cccagcaact
     4621 gctccgcgtt gagccacctc cagttcttcg actcgtggct gtcgatcacg acccgccgcg
     4681 acccgaacgt gtacaagaac ggccaggtct ccgggtaccg cggcggcgac ggcacgtcgt
     4741 tcctccatgc ccccgatccc gatgccgatc tccacctccg caaggactcc tcgggtcact
     4801 ggatcccg
//
LOCUS       DATMFS010000197         4781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_77311, whole genome shotgun sequence.
ACCESSION   DATMFS010000197 DATMFS010000000
VERSION     DATMFS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77311"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNQ33_03620"
     CDS             <1..609
                     /locus_tag="VNQ33_03620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97222.1"
                     /translation="GAVADLHRRAFPSYASTKLGAGYCRRMLRAYADRPGAWIDVAVG
                     ADGRIIGYLVAAPPATQRAVDRALLPWAALNAFRRPGDLVGNVRRFGGRISRRRRAAD
                     AGPAAPGAVAGPPVGARQPPGAATVRVVLVAVDAAARGQGGADALLDAFARTARGRGH
                     LGADLSVAPGNGAAHGAYARNGWRPDGTGTRFTLDLSGPAAP"
     gene            complement(587..1294)
                     /locus_tag="VNQ33_03625"
     CDS             complement(587..1294)
                     /locus_tag="VNQ33_03625"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HWJ97223.1"
                     /translation="MLALIPARGGSQGIPRKNLAEVGGRSLVAWAVAAAQESARVDRI
                     IVSTDDDEIAAAATAAGAEVPFLRPSHLAGHDTTDLPVFVHALDWLATEQGQHPSIVV
                     HLRPTSPARRRGLVDDAIAHLQAHPEASSLRSVSPSPLTPWKMYEERDGFLAPLLGSI
                     EQEAFNQPRQQLPPAWIHDGVIDVVRTETLRAGSMSGPRILAWHSDAAEAVDIDHPED
                     LARADAAVRSVRERPDR"
     gene            complement(1300..2763)
                     /locus_tag="VNQ33_03630"
     CDS             complement(1300..2763)
                     /locus_tag="VNQ33_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWJ97224.1"
                     /translation="MTSPAEVDDPDGLDLAPPPLATFDTAPKRLLIPRWMLLVGLIIA
                     QALLARYMTQIPRLGQIQAVMILALVGYGALKRNTPLMLCIAAYLPGAEIVWRQAHVT
                     LPYLFAPYTLTAIAVLSLITCYPSINRIGREALLYFALLIPSSLITFSIAQHGGRKLV
                     SFALSGAAALAALTMLFSQVAIRPWLYRRLLWVMLISGVGPLSVALTAISDYIVNVGT
                     LEFNGESNSVTSGGFGPVQVSSLMGLTVLVAVLLVLAERDVATRVIAGVVGIWSATQS
                     LLTFSRGGMFAVAIAVAGLVVVQAWNREARRKVFALVGVSLLIGYYIIIPRLDAFTRG
                     GLEERFSDTQTGRTTLAHNDIQIFLEHPVFGVGPGMSKYRRLPYEICELRSDRCNYEG
                     SSHTEFTRMIAEHGSVGLAAMAILLLLAFHAIRRAGPSLAFTVVMLLWAIAQMVYANM
                     RIAAVPFAFALAFIQIRDPNDDPVTEEPDPTLEPAIS"
     gene            complement(2765..4522)
                     /locus_tag="VNQ33_03635"
     CDS             complement(2765..4522)
                     /locus_tag="VNQ33_03635"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWJ97225.1"
                     /translation="MLATKDTNEVSLDEYVSVLRRRWLWIVLTPLIFGGLAFAKDVRA
                     ERVYSASTQLLLRTNQSEQFLNANPTVQSLPERALQNELRLITSRKVKLAVADAYGKP
                     ITVRAAAGGEDDVIVLTASSNSGKVAAEQANAYAETYQDVRLETQLEDLNDARDIVQQ
                     QVDDFQKQVDAFNEPLAKLDEQILALPAGDPRYQTLVDTREQVRSRTESERNDAQNQL
                     NEYKQRLQLLDVNERLQTDGGIIVLNPATVPSSPTSPNTFQDVAQALIVGVFVGLALA
                     FARDQLDDSLRSKADVERAVRGVPTLALVPYDAASNDVRTPRISTLIAPKSATAESYR
                     GLRTTMQYAALERPMRTVQVTSGSAGEAKTTTITNLAMAFALSGKRVALVDCDLRKPK
                     LHRFLEVDGSKGFTSVVLGELPLQEALQTSPLHPNIDVLPSGFLPPNPSELLSHDRTA
                     RIFQTLTEHYSIVFIDCPPVLPVTDSLVISRYVDATLFLVMADQTSRRTARRAVEMLR
                     QVGAPLLGTVINGAADQETYGSLYEYYGYVKRSNVPLVGRFIKRKAVDIPSATEDILP
                     QVDDVTATSDELDPEQVST"
     gene            complement(4569..>4781)
                     /locus_tag="VNQ33_03640"
     CDS             complement(4569..>4781)
                     /locus_tag="VNQ33_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97226.1"
                     /translation="LAAALRSLLDAGPRARTAMGERGRHVAEEMFDQVQVTRQIERIY
                     HHVIIGGTDGATPEPARPIPAARSDR"
BASE COUNT          679 a         1524 c         1860 g          718 t
ORIGIN      
        1 ggcgccgtcg ccgacctgca ccgtcgggcg ttcccctcct acgcgagcac gaagctgggc
       61 gccggctact gccgtcggat gctgcgggcc tacgccgacc ggcccggcgc ctggatcgac
      121 gtcgcggtcg gcgccgacgg ccggatcatc ggctacctcg tggccgcacc gccggccacg
      181 cagcgcgccg tcgaccgggc gctgctcccc tgggcggcgc tgaacgcctt ccgccgcccg
      241 ggcgacctgg tcggcaacgt ccgccggttc ggaggtcgga tctcccgtcg ccgccgcgcc
      301 gccgacgccg gccccgcggc ccccggtgcc gtcgccggtc ctcccgtcgg agcacgccag
      361 ccgcccggcg cggcgacggt gcgcgtcgtg ctcgtggccg tcgacgccgc ggcccgtggc
      421 cagggcgggg ccgatgcgct cctcgacgcc ttcgcccgga ccgcgcgcgg ccgcggccac
      481 ctcggggccg atctgagcgt cgcccccggc aacggtgcgg cccacggcgc ctacgcccgc
      541 aacggatggc gacccgacgg cacgggcacc cgcttcaccc tcgacctcag cggtccggcc
      601 gctccctgac ggagcggacg gcggcgtcgg cgcgcgccag gtcctcggga tggtcgatgt
      661 cgaccgcctc ggcggcatcg gagtgccatg ccaggatccg gggtcccgac atcgagccgg
      721 cccggagcgt ctcggtccgg accacgtcga tgacgccgtc gtggatccac gccggcggca
      781 gctgctgacg gggctggttg aacgcctcct gctcgatcga tccgagcagc ggggcgagga
      841 agccgtcgcg ctcctcgtac atcttccagg gcgtgagcgg cgacgggctc accgagcgca
      901 gcgacgatgc ctcggggtgc gcttggaggt gggcgatggc gtcatcgacg aggccacgcc
      961 ggcgggcggg cgaggtgggg cggaggtgga ccacgatgga cgggtgctgg ccctgttcgg
     1021 tcgccagcca gtcgagggcg tgcacgaaga ccggcaggtc ggtggtgtcg tgtccggcga
     1081 ggtggctcgg gcgcaggaac ggcacctcgg cgccggcggc ggtggccgcg gcggcgatct
     1141 cgtcgtcgtc ggtgctgacg atgatgcggt cgacccgtgc cgattcctgc gccgcggcca
     1201 cggcccaggc gaccaggctc cggccgccga cctcggcgag gttcttgcgg gggatgccct
     1261 ggctgccacc tcgcgccggg atcagcgcga gcacgcgcat cacgagatcg cgggctcgag
     1321 cgtcgggtcc ggctcctcgg tgaccgggtc gtcgttcgga tcgcggatct ggatgaacgc
     1381 gagcgcgaac gcgaacggga ccgcggcgat gcgcatgttg gcgtagacca tctgcgcgat
     1441 ggcccagagg agcatgacga cggtgaacgc gagcgacggt ccggcgcgcc ggatcgcgtg
     1501 gaacgcgagc aggaggagga tggccatggc ggcgaggccg acgctgccgt gctcggcgat
     1561 catgcgggtg aactcggtgt gggacgagcc ctcgtagttg caccggtcgg agcgcagctc
     1621 gcagatctcg tagggaaggc gccggtactt ggacatgccg ggacccacac cgaagaccgg
     1681 gtgctcgagg aagatctgga tgtcgttgtg cgccagcgtg gtccggcccg tctgcgtgtc
     1741 ggagaaccgt tcctcgagac cgcctcgcgt gaacgcgtcg agcctcggga tgatgatgta
     1801 gtagccgatg agcaagctga cgccgacgag ggcgaacacc ttccgccggg cctctcggtt
     1861 ccacgcctgc acgacgacca ggccggccac ggcgatggcc acggcgaaca tgccgccgcg
     1921 cgagaaggtg agcaggctct gggtggccga ccagatgccg acgacccccg cgatcacgcg
     1981 cgtcgccaca tcccgttcag ccagcaccag caagacggct acgagcaccg tgaggcccat
     2041 gagactggac acctgcacgg ggccgaagcc gccgctggtg acgctgttcg actcgccgtt
     2101 gaactccagc gtgcccacgt tgacgatgta gtcgctgatg gcggtgaggg cgaccgacag
     2161 cgggccgacg ccgctgatca gcatcaccca gagcagccgc cggtacagcc acggccggat
     2221 ggccacctgc gagaagagca tggtgagcgc ggcgagggcc gcggccccgg agagggcgaa
     2281 cgagaccagc ttgcgcccgc cgtgctgggc gatcgagaac gtgatcagcg acgaggggat
     2341 caagagcgcg aagtacagga gcgcctctcg gccgatccgg ttgatcgagg ggtagcacgt
     2401 gatcagcgag agcaccgcga tggcggtcag cgtgtaggga gcgaacaggt acgggagggt
     2461 gacgtgggcc tggcgccaca cgatctcggc gccgggcagg taggcggcga tgcagagcat
     2521 gagcggggtg ttccgcttga gggccccgta gccgaccagg gcgaggatca tgaccgcctg
     2581 gatctgcccg aggcgcggga tctgggtcat gtaccgcgcc aggagggcct gggcgatgat
     2641 gaggccgacc agcagcatcc accgggggat gaggagccgc ttgggggcgg tgtcgaaggt
     2701 cgcgagcggg ggcggagcca ggtcgaggcc gtcggggtcg tcgacctccg cggggctggt
     2761 catctcaggt ggagacctgt tccggatcga gctcgtcgct cgtcgccgtc acgtcgtcga
     2821 cctgcggcag gatgtcttcg gtggccgagg ggatgtcgac ggccttgcgc ttgatgaagc
     2881 ggcccacgag cgggacgttc gagcgtttga cgtagccgta gtactcgtag agcgagccgt
     2941 aggtctcctg gtcggccgcg ccgttgatga cggtgccgag gagcggggcg ccgacctggc
     3001 gcagcatctc gaccgcccgg cgggcggtcc gcctcgaggt ctggtcggcc atgaccagga
     3061 acagggtcgc gtcgacgtag cggctgatga ccagcgagtc ggtgacgggg aggaccggcg
     3121 ggcagtcgat gaagacgatc gagtagtgct cggtcagcgt ctggaagatg cgagccgtcc
     3181 ggtcgtggct gagcagctcg gacgggttcg gcgggaggaa gcccgagggg agcacgtcga
     3241 tgttcgggtg cagcggcgag gtctgcaggg cctcctgcag cggcagctcg cccagcacga
     3301 cggaggtgaa gcccttggag ccgtcgacct cgaggaagcg gtggagcttc ggcttgcgga
     3361 ggtcgcagtc gacgagggcg acccgcttgc ccgacagggc gaacgccatc gccaggttgg
     3421 tgatggtggt ggtcttggcc tcgccggcgc tgccgctcgt gacctggacc gtgcgcatcg
     3481 gccgttccag ggccgcgtac tgcatggtgg tcctcaggcc gcggtaggac tcggcggtgg
     3541 cggacttggg agcgatgagc gtgctgatgc gaggcgtgcg cacgtcgttg ctggcggcgt
     3601 cgtaggggac cagcgccagc gtcgggacgc cgcggaccgc ccgctccacg tcggccttgg
     3661 agcgaagcga gtcgtcgagc tggtctcggg cgaacgccag ggcgaggccg acgaagacgc
     3721 cgacgatgag ggcctgggcg acgtcttgga aggtgttcgg cgaggtcggc gagctcggca
     3781 ccgtcgccgg gttgagcacg atgatgccgc cgtcggtctg gagccgttcg ttgacgtcga
     3841 ggagctggag gcgctgcttg tactcgttca gctggttctg ggcgtcgttg cgctcggatt
     3901 cggtccgcga ccgcacctgc tcacgggtgt cgaccagggt ctggtagcgc gggtcgccgg
     3961 cgggcagggc gaggatctgc tcgtcgagct tggccagcgg ctcgttgaag gcgtcgacct
     4021 gcttctggaa gtcgtcgacc tgctgctgga cgatgtcgcg ggcgtcgttc agatcctcga
     4081 gctgcgtctc gaggcggacg tcctggtagg tctcggcgta cgcgttggcc tgctcggcgg
     4141 cgaccttgcc gctgttcgac gacgcggtga gcacgatgac gtcgtcttcg ccaccggccg
     4201 cagcgcgcac ggtgatcggc ttcccgtagg catcggcgac ggcgagcttc accttgcggc
     4261 tggtgatcag gcgcagctcg ttctggagcg cccgctccgg caggctctgc accgtggggt
     4321 tggcgttgag gaactgctcc gactggttgg tgcggagcag cagctgcgtc gacgcggagt
     4381 agacccgctc ggctcggacg tccttggcga aggcgagccc gccgaagatc agcggggtga
     4441 ggacgatcca gagccatcga cgtcggagca ccgacacgta ttcgtcgagg ctgacttcgt
     4501 tggtgtcctt ggtggcgagc atgggtccgt ttcaggttgg tcagcgagcc gagcgcagcg
     4561 ttcgagtatt accgatcgga gcgggccgcg gggatcggtc gggcgggttc gggggtggcg
     4621 ccgtcggtgc caccgatgat cacgtggtgg tagatccgct cgatctgccg ggtgacctgc
     4681 acctggtcga acatctcctc ggcgacgtgt cgaccgcgct cgcccatcgc ggtgcgggcg
     4741 cgggggccgg catcgagcag ggatcggagg gcggcggcga g
//
LOCUS       DATMFS010000198         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_194251, whole genome shotgun sequence.
ACCESSION   DATMFS010000198 DATMFS010000000
VERSION     DATMFS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194251"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VNQ33_03645"
     CDS             <1..661
                     /locus_tag="VNQ33_03645"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HWJ97227.1"
                     /translation="KRYVEFMGGADAVLEKARASFDDGDYRWVAEVVSHVVFADPANE
                     AARALEADALEQLGYQAESGPWRDFYLTGAQELRNGHPELGISSTAAPDTVAAMTTEM
                     LLDYLGVRIDGLAAATRSWAIDLTATDRGERWRVGIERGALHSIRLEPGPEDLPDTDV
                     AIEADHAPLAQLLFAPEPVAELAALVDAGSVTVTGDRPGLEAFLATLDSFALMFPIVT
                     P"
     gene            777..1334
                     /locus_tag="VNQ33_03650"
     CDS             777..1334
                     /locus_tag="VNQ33_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWJ97228.1"
                     /translation="MAEDRSAISIVRESKILAYTVGAMTLAAGLVLLFWPDRTLTVVA
                     RLAGILLVVVGIGDLLETIRHHRGMSYWGLLLLRAVINIGFGLVLLLWPSITVGVMVW
                     LVGLDLVIAGAIGLLARGQMGPEFKSAITSRSILTILFGIVIMVWPHATVSVLAFLVA
                     AMLVLFGLVFLWSGYQLSKARVIEV"
     gene            complement(1359..2183)
                     /locus_tag="VNQ33_03655"
     CDS             complement(1359..2183)
                     /locus_tag="VNQ33_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ97229.1"
                     /translation="MITHLPGIGVTVISKWLYNCYVIHDGGGGQPFVVDLGMPSQLPL
                     VADALRAHGSDASELGAAVATHGHADHVGGLPALRQAHGTAVHLPRAIAELRDGTRPL
                     RPPGPRAVAEILPVMASQPRDLVATKEIAGTMKEIGWDGKGVRLPFEPDSWLADGDHL
                     PGFPDWQVLTTPGHSDDSTCLYHAPTRTLLSGDAVLSVEGRGWFNPEYVDGRLSVQTE
                     ARLRLLDVEHLLPGHGLVVQGPDVMNTALGFTEKPREHSKLKALYRVLSNHATRHD"
     gene            complement(2213..>2534)
                     /locus_tag="VNQ33_03660"
     CDS             complement(2213..>2534)
                     /locus_tag="VNQ33_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HWJ97230.1"
                     /translation="PTPTPTPAPAALVAPMGYVAPVAGVYLPVVTTPPKSKIAAGLLA
                     FFLGTLGIHRFYLGYNGIGITMLLLTVLSFGILAPIVAIWALVECIVIFAGGMKDSHN
                     RPLG"
BASE COUNT          342 a          884 c          922 g          386 t
ORIGIN      
        1 gaagcgctac gtcgagttca tgggcggcgc cgacgccgtc ctcgagaagg cccgagcctc
       61 gttcgacgac ggcgactacc gctgggtggc cgaggtcgtg agccacgtcg tgttcgccga
      121 tccggccaac gaggcggccc gcgccctgga ggccgacgcc ctcgagcagc tcggctacca
      181 ggccgagtcc gggccctggc gcgacttcta cctgaccggt gcccaggagc tgcgcaacgg
      241 ccacccggag ctggggatca gctccacggc cgcgcccgac accgtcgccg ccatgaccac
      301 cgagatgctc ctcgactacc tcggcgtgcg gatcgacggc ctggccgcgg ccacccggtc
      361 ctgggcgatc gacctcaccg ccaccgaccg cggcgagcgc tggcgggtcg gcatcgagcg
      421 gggtgcgctc cacagcatcc gcctggagcc cggacccgag gatctgcccg acaccgatgt
      481 cgccatcgag gccgaccacg ctccgctggc ccagctcctg ttcgccccgg agcccgtggc
      541 cgagctcgcc gccctcgtcg acgccggctc ggtgaccgtc accggtgacc ggcccgggct
      601 cgaggccttc ctcgccaccc tggactcgtt cgccttgatg ttccccatcg tcaccccctg
      661 aggtcggacc agggcgaggg accgcccccg ccctgggtcg accaggggtc acccggacgg
      721 ggctaccgtc aggggcgcgg gcgacggcgc ccggcaggga cagcaggagg gtcggcatgg
      781 ccgaagaccg ctcagccatc agcatcgtgc gggagtcgaa gatcctcgcc tacaccgtcg
      841 gggcgatgac cctggccgcg ggtctcgtgc tgctcttctg gcccgaccgc acgctcaccg
      901 tcgtggcccg cctcgccggc atcttgctcg tggtcgtcgg catcggcgac ctgctcgaga
      961 ccatccgcca ccaccgcggc atgtcctact gggggctgct gctgctgcgt gcggtcatca
     1021 acatcgggtt cggcctggtc ctgttgctct ggccgagcat caccgtcggc gtgatggtgt
     1081 ggctcgtggg cctcgacttg gtcatcgccg gcgccatcgg tctcctcgcc cgagggcaga
     1141 tgggacccga gttcaagtcg gccatcacca gccgctcgat cctgacgatc ctcttcggca
     1201 tcgtgatcat ggtctggccc cacgccaccg tcagcgtgct cgccttcctc gtcgcggcga
     1261 tgctcgtcct cttcggcctg gtcttcctct ggtcgggcta ccagctcagc aaggcgcgcg
     1321 tgatcgaggt gtagcggcct tcggctctcg cgccgtgctc agtcgtgacg ggtggcgtgg
     1381 ttgctgagca cccggtagag ggccttcagc ttggagtgct cgcgtggctt ctcggtgaag
     1441 ccgagggcgg tgttcatcac gtcggggcct tgaacgacga ggccgtggcc gggcaggagg
     1501 tgctcgacgt cgagcaggcg gagccgggcc tcggtctgca ccgacaggcg gccgtcgacg
     1561 tactcggggt tgaaccagcc cctgccctcg accgacagca cggcgtcgcc ggacagcagc
     1621 gtgcgcgtgg gggcgtggta gaggcaggtc gagtcgtcgc tgtggccggg cgtggtgagg
     1681 acctgccaat cggggaagcc gggcaggtgg tcgccgtcgg ccagccacga gtcgggctcg
     1741 aacgggaggc gcacgccctt gccgtcccag ccgatctcct tcatcgtccc ggcgatctcc
     1801 ttcgttgcca ccaggtcacg gggctggctc gccatcaccg ggaggatctc ggccaccgcc
     1861 ctcggcccgg gcgggcgcag ggggcgggtg ccgtcgcgca gctcggcgat ggcgcggggg
     1921 aggtgcaccg cggtgccgtg ggcctggcgc agcgcgggca ggccgccgac gtggtcggcg
     1981 tggccgtggg tggcgacggc ggcgccgagc tcggatgcat cggagccgtg cgcgcggagc
     2041 gcatcggcca cgagcgggag ctgcgacggc atgccgaggt cgaccacgaa gggctggcca
     2101 ccgccgccgt cgtggatcac gtagcagttg tagagccact tcgagatcac ggtcaccccg
     2161 atcccgggca ggtgcgtgat cacgggttcc ggcgcgcggc gagccatcgg gttcagccca
     2221 gaggccggtt gtgcgagtcc ttcatgccgc cggcgaagat gacgatgcat tcgaccagcg
     2281 cccagatcgc aacgatcggg gcgaggatgc cgaagctcaa caccgtcagc aggagcatgg
     2341 tgatgccgat gccgttgtag ccgaggtaga agcggtggat gcccagcgtg ccgaggaaga
     2401 aggcgagcag cccggcggcg atcttcgact tcgggggcgt ggtcacgacg ggcaggtaga
     2461 cgccagcgac cggcgcgacg tatcccatgg gcgccacgag ggcggccggt gccggcgtcg
     2521 gcgtcggcgt cggc
//
LOCUS       DATMFS010000199         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_77353, whole genome shotgun sequence.
ACCESSION   DATMFS010000199 DATMFS010000000
VERSION     DATMFS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77353"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="menD"
                     /locus_tag="VNQ33_03665"
     CDS             complement(<1..1186)
                     /gene="menD"
                     /locus_tag="VNQ33_03665"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HWJ97231.1"
                     /translation="MSDDLRPQDIQAAFCAALVDEWVRGGVTDAVVCPGSRSTPMALA
                     LAADDRIAVHVHHDERAGGFVALGLGLASGRPALVLTTSGTAAVELHPSVVEAHQASV
                     PVIVVTADRPPELHHVGAPQTVDQARLFGGAVRWFADPGVPGAAASGTWRSLAARSVA
                     EATTGVAGPGPVHLNLAFREPLVGTAGELPPGRPDGAPWHTTTGKRSAVDRLGTARLE
                     ELLDADRGVIVAGAGAGDPAVVQSLSAATGWPVLADPRSGCRNPAAGVVAAFDALLRH
                     QPTASALAPQVVLRLGAPPASKVLGQWIAASGAVEVAVDEHGRWFDPTHQAAHVLHAD
                     PAAACQALVQLVGGRSNEAWSAAWAAAEAAAQRALDAVLADHIEPTEPGIARTVAAAL
                     PEG"
     gene            1222..1977
                     /locus_tag="VNQ33_03670"
     CDS             1222..1977
                     /locus_tag="VNQ33_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ97232.1"
                     /translation="MPAAPPRIVLLHGFTQTGTSWRPIAERLPPGWDVTAPDLPGHGT
                     AGALRLDLAASAARLAEQIGSEPALVVGYSMGGRTALRLALDHPDAVAGLVLVGATAG
                     IDDPGERAARRRVDGERADRIEAEGVEPFLDRWLAQPLFASLRPRPDDLAARRANTAA
                     GLASSLRLAGTGTMDPPWWDELPRIDVPALVLWGEADTKFAALGRRLAGGIGASAETT
                     SLVDVGHAAHLEASDAVAGLVTGWARRTLGYER"
     gene            complement(1981..2862)
                     /locus_tag="VNQ33_03675"
     CDS             complement(1981..2862)
                     /locus_tag="VNQ33_03675"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HWJ97233.1"
                     /translation="MNIWVAGARPRTLPAAVVPVAVGTACAVGEVPGGLIWWRAAAAM
                     VVALALQVATNYANDYSDGIRGTDADDKRVGPVRLVGQGLAEPGSVKRAALLSFAVAG
                     LVGTALALAVGPELFVVGAAAIAAGWFYTGGPKPYGYAGLGEAFVFVFFGVVATAGSA
                     YVQTGSLRALALGASIPVGLLATALLVVNNLRDIPGDTEVGKRTLAVRLGDQRTRTLY
                     AAMLVAAFAVVPLVAGLSDRPVAAIALLAVFVARKPVFEVLSGARGPALIPVLGETGK
                     AQLAFGLLFTIGLAIVV"
     gene            3008..>3420
                     /locus_tag="VNQ33_03680"
     CDS             3008..>3420
                     /locus_tag="VNQ33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97234.1"
                     /translation="MRRLIALDLPGGSDFVLALRRCWANGDAVLVLDPRLPAIARTTL
                     IGAARPHHAITAPGADPVPLDPAAPPVEDGDALVVTTSGSTGEPKVLVHTHDSLLAHA
                     GAVHERLEVDPARDRWLATLPLNHLGGFGVVARSVL"
BASE COUNT          452 a         1348 c         1251 g          369 t
ORIGIN      
        1 tgccctcggg cagggcggcg gcgacggtcc gggcgatccc gggctccgtc ggctcgatgt
       61 gatcggccag cacggcgtcg agggcgcgct gcgccgccgc ctcggctgcg gcccacgcgg
      121 ccgaccacgc ctcgttcgac cgcccgccta cgagttggac gagcgcctgg cacgccgccg
      181 ccggatcggc gtggagcacg tgggcggcct ggtgggtggg gtcgaaccac cggccgtgct
      241 cgtcgaccgc cacctcgacg gcgccggagg cggcgatcca ctgcccgagc accttcgagg
      301 ccgggggcgc accaagccgc agcacgacct ggggcgcgag cgccgacgcc gtgggctggt
      361 ggcggagaag cgcatcgaac gccgccacca cgccggcggc cgggttccgg caccccgacc
      421 gggggtcggc cagcaccggc caccccgtcg cagcagacag ggactggacc acggccgggt
      481 caccagctcc cgcacccgca acgatcacgc cgcgatccgc gtcgagcagc tcctcgaggc
      541 gcgcggtccc gagccggtcg accgccgagc gcttgccggt cgtggtgtgc cagggcgcgc
      601 cgtcgggtcg accgggcggc agctcgccgg cggtcccgac gagcggctcg cggaacgcca
      661 ggttcaggtg caccggcccg ggaccggcga caccggtggt ggcctccgcc accgaccggg
      721 cggcgagcga gcgccaggtg ccagacgccg ccgcgccggg cacgcccggg tcggcgaacc
      781 accgcaccgc accgccgaag aggcgggcct ggtcgacggt ctgcggcgcg cccacgtggt
      841 gcagctcggg cggccggtcc gccgtcacga cgatcacggg cacggacgcc tggtgggcct
      901 cgaccacgct cgggtgcagc tcgacggccg ccgtcccgct ggtggtgagc acgagcgccg
      961 gccgccccga cgccaggccg agcccgagcg ccacgaagcc gccggcccgc tcgtcgtggt
     1021 gcacgtggac ggcgatgcgg tcgtcggccg ccagggcgag ggccatcggc gtggagcgcg
     1081 agccggggca cacgaccgcg tcggtgaccc cgccgcgcac ccactcgtcg acgagcgcgg
     1141 cgcagaaggc ggcctggatg tcctgcggtc ggaggtcgtc gctcacctgc ccatctgagc
     1201 acagggtgcg aggatgcagc gatgcccgcc gcgcccccac ggatcgtcct cctccacggg
     1261 ttcacccaga ccggcaccag ttggcggccg atcgccgagc ggcttccgcc gggctgggac
     1321 gtgacggcac ccgacctgcc ggggcacggc accgccggcg cgctgcgcct ggacctggcg
     1381 gccagcgccg cccggctggc cgagcagatc ggcagcgagc cggccctcgt ggtcggctac
     1441 tcgatgggcg gccgcacggc cctgcgcctc gccctcgacc accccgatgc cgtcgccggc
     1501 ctggtcctcg tcggcgccac cgcgggcatc gacgaccccg gcgagcgcgc tgcccggcgg
     1561 cgggtcgacg gcgagcgcgc cgaccgcatc gaggcggagg gtgtcgagcc gttcctcgac
     1621 cggtggctcg cccagcccct gttcgccagc ctgcgccccc ggcccgacga cctcgcggcc
     1681 cgccgggcga acaccgcggc cggcctggcg tcgtcgctcc gcctggccgg gaccgggacc
     1741 atggatccgc cgtggtggga cgagctgccc cgcatcgacg tgccggccct cgtcctgtgg
     1801 ggcgaggccg acaccaagtt cgccgccctc ggccgccgcc tggccggggg catcggcgcg
     1861 agcgccgaga cgacctcgct ggtcgacgtc ggccatgccg cccacctcga agcgagcgac
     1921 gccgtcgccg gcctcgtcac cggctgggcc cgccgcaccc tcggctacga gcggtgagcc
     1981 tcagacgacg atcgccaggc cgatcgtgaa gaggaggccg aacgcgagct gcgccttgcc
     2041 ggtctcgccg agcaccggga tgagcgccgg gccacgagca cccgagagca cctcgaacac
     2101 cggcttgcgg gccacgaaca cggcgagcag cgcgatggcg gcgaccggcc ggtcgctcag
     2161 ccccgccacc aacgggacca cggcgaaggc ggcgaccagc atcgcggcgt agagggtgcg
     2221 ggtgcgctgg tcgccgaggc ggacggcgag ggtccgcttg ccgacctcgg tgtcgcctgg
     2281 gatgtcgcgc aggttgttca cgacgaggag ggccgtggcg agcaggccca cggggatcga
     2341 agcaccgagc gcgagcgcac ggagcgatcc cgtctggacg tacgcggacc cagccgtggc
     2401 gaccacgccg aagaagacga acacgaacgc ctcgccgagc ccggcgtacc cgtacggctt
     2461 gggaccgcct gtgtagaacc acccggcggc gatggcggcg gcgcccacca cgaacagctc
     2521 ggggccgacg gcgagggcga gcgcggtgcc caccagaccg gcgacggcga acgacagcag
     2581 cgccgccctc ttgaccgagc ccggctcggc gaggccctgg ccgaccagcc gcaccggccc
     2641 gacgcgcttg tcgtcggcgt cggtcccgcg gatgccgtcg ctgtagtcgt tggcgtagtt
     2701 cgtggcgacc tgcagcgcga gggccacgac catggccgcc gccgcccgcc accagatcag
     2761 cccgcccggc acctcgccca ccgcgcacgc cgtcccgacc gccacgggca cgaccgcagc
     2821 cggcagcgtc cgaggccgag caccagccac ccagatgttc atggccccga gtctggccgc
     2881 accaccgtct ccaccccgaa ccccgacccc acccgatgcg aacttcgtag gtgagaacgc
     2941 gcatccggga cctctcacct acgaagttcg gcgatggcgg tgcggtggat tctgcgagga
     3001 tggaccggtg aggcggctga tcgcgttgga cctaccgggc gggtccgact tcgtgctggc
     3061 cctgcggcgc tgctgggcga acggcgatgc cgtgctcgtg ctcgaccccc ggctgcccgc
     3121 catcgcccgc acgacgctca tcggcgccgc ccgtccccac cacgccatca ccgctcccgg
     3181 cgccgacccg gtgccgctcg atccggcggc gccgcccgtc gaggacggcg atgcgctcgt
     3241 cgtcacgacc agcgggtcga ccggcgagcc caaggtcctg gtccacaccc acgactccct
     3301 cctcgcccac gccggcgccg tgcacgagcg cctggaggtg gacccggccc gggaccggtg
     3361 gctggccacg ctgcccctca accacctggg cggcttcggc gtggtcgccc ggtcggtcct
//
LOCUS       DATMFS010000200         5849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_333766, whole genome shotgun sequence.
ACCESSION   DATMFS010000200 DATMFS010000000
VERSION     DATMFS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333766"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VNQ33_03685"
     CDS             complement(<1..1806)
                     /locus_tag="VNQ33_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWJ97235.1"
                     /translation="MPGRTLSEADSKILLAGHGVPFAPERVVATAEEAVTAAVDVGLP
                     VVVKLNGDAIAHKTERGLVHLDLADEDAVRAAARSLLDAATPEDGEVGLLVAPMLKGN
                     RELIAGLADDPQFGMTVMVGIGGILAEAIADVAIRPVPITDVDAAEMIDELGTQALLG
                     AFRGEAAVDRTALAQVLLGLSSAAEADASIRSADLNPLIVVDGTPIAVDALVEVGDPA
                     SAAGGAKKRPTDAGFRALFDPKGVVVAGASSHPGKFGFVSLHNVLASGYEGAVGATNR
                     ERAEVLGVETVADIAELPDDTFDLIFVCTPASVNPELLRAAAAKGITAAFVTSAGYGE
                     AGEDGIAAQAELVALCDELGILLAGPNGQGVVSTPSKLCAQIVAPYPPAGSIGVASQS
                     GNFVSSFLNYAVQTGVGISRAVSAGNAAAVTVPDYLDFYADDPATAVGLAYVEGVPDG
                     RAFLDRMSSVARRKPLVLVKGGATSGGQRAAASHTGSLATDDRVFDGACRQAGVTRAA
                     TVEEAFEAAATFATQPLPKGARVVVMTTAGGWGVVTADAITRSDLELAELPADLLAAI
                     DQKLPPRWSRNNPVDLAGGETRDTIPEVLELIAAHD"
     gene            complement(1886..2506)
                     /locus_tag="VNQ33_03690"
     CDS             complement(1886..2506)
                     /locus_tag="VNQ33_03690"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ97236.1"
                     /translation="MAATEPVLSTREAIIVEARRCFAEHGYEGTSLNEIAEAVGIRRP
                     SLLHHFPSKEAVYAEVFRISLAEWSIRVEDAVGRGVEGWQKVDLVITTGFRFFQENPE
                     FVRIISREALDDGANLGIDLGAALRPYLQRAMGWFEREIEAGHFRRVDTEQLLITAYS
                     IMFGYFSEAPFLTSLLDRDPTSPAALEARLKHVRNLFHVALSPTAP"
     gene            2613..3008
                     /locus_tag="VNQ33_03695"
     CDS             2613..3008
                     /locus_tag="VNQ33_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97237.1"
                     /translation="MDIVAAVFIENIELRQVEGPSTRIDLTGVHFSLAAPAPVPVTME
                     PHLVVLVRCAPDEAGTGALETVYQRDGEQVARNVQPLQVEPGKFAYRLVRAELTFDDY
                     GTVEAEIRIDQGHITTVPFTLLPPVAAGS"
     gene            3031..4179
                     /locus_tag="VNQ33_03700"
     CDS             3031..4179
                     /locus_tag="VNQ33_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWJ97238.1"
                     /translation="MSQHPLATHAVGEVVGEVLDRLGDEPDAAAVFVTSPFAGAMEDI
                     AATVRTLLRPASLIGATAASVLAGEREVEGEAAVSLFAMRFGPRRRFHSPPVTEAVRI
                     RAEREDDGWRVEASGSAAFAGSTLVLLADPFSFPVETFIEDLGTRRPGLTVVGGLASS
                     STGPGGNRLVADGRVFSDGAVGLLLPAGVGVTPVVSQGCRPVGEPLVVTKASGNLLEE
                     IAGQPALDRLMAQADAATTEDRNRMARGLHVGLVVDERKAEFDRGDFLIRSVLGADHA
                     RKAVAVGAEVEVGATVQFQVRDADSADEDLRDLLAGTRGDAALLFTCTGRGTALFGTP
                     HHDARIVSQHVDGGPVAGMFCAGEVGPLGDRTFVHVLSATALLFDDPF"
     gene            complement(4176..4724)
                     /locus_tag="VNQ33_03705"
     CDS             complement(4176..4724)
                     /locus_tag="VNQ33_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97239.1"
                     /translation="MGDRPFELGGVNHLALVCRDMQATVDFYEGILGMPLVKTIELPV
                     GLGQHFFFDCGGGDCLAFFWFPDAQEPQAGVSGPGCRPDQGALASAIGSMNHVAFQVP
                     AERLEEYQAKLRAAGVETSEIANHDDSEFGLADEWNGDVFVRSLYFLDPDGILLEFAA
                     WTRALGQPGDVVHQPNRAVTRI"
     gene            complement(4827..>5849)
                     /locus_tag="VNQ33_03710"
     CDS             complement(4827..>5849)
                     /locus_tag="VNQ33_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ97240.1"
                     /translation="EAVGVVGAIIPWNVPLFEAVMKVAPAVIAGCTVVLKPSPETALD
                     PYVFAEIMEEAGLPKGVLSIVPGGREIGEYLVTHPLVDKITFTGSTAAGMKIGAACGA
                     LLRPVTLELGGKSAAMVLDSADVAAIVPQLLDAGIMNNGQACVAQTRILASRENYANV
                     LDAVTEAVAALVVGDPLDPATQVGPVVAERQRERILGLLDTGRSEGARVTTGGGRTSH
                     EKGWFIEPTVFGDVDNKMTIAQEEIFGPVLSVIPFDDVEDAVRIANDSQYGLSGSVWS
                     ADVDEATAIAKRIRTGTINVNHFAMAFGAPFGGYKNSGLGRELGPEGVDAFMEKKALS
                     LDPAAG"
BASE COUNT          806 a         2049 c         2144 g          850 t
ORIGIN      
        1 gtcgtgggcc gcgatcagct cgaggacctc ggggatcgtg tcgcgggtct cgccgccggc
       61 gaggtccacg gggttgttgc ggctccagcg gggcgggagc ttctggtcga tggcggcgag
      121 gaggtcggcg ggaagctcgg ccagctccag gtcgctgcgg gtgatggcgt cggcggtgac
      181 caccccccac ccgccagccg tggtcatcac gacgacccgg gcgcccttcg gcagcggctg
      241 ggtggcgaac gtcgccgccg cctcgaacgc ctcctccacg gtggccgcgc gggtcacgcc
      301 ggcttggcgg caggcgccgt cgaacacccg gtcgtcggtg gccagcgacc cggtgtggct
      361 cgccgccgcc cgctgaccgc cggacgtggc gccgcccttc acgaggacca gcggcttgcg
      421 tcgggcgacc gacgacatgc ggtcgaggaa cgcccggccg tccggcacgc cctcgacgta
      481 ggcgagcccc acggccgtcg ccgggtcgtc ggcgtagaag tcgaggtagt cgggcacggt
      541 gaccgccgcg gcgttgccgg cgctgaccgc tcggctgatg cccacgcccg tctgcaccgc
      601 gtagttgagg aacgacgaga cgaagttgcc cgactggctg gccacgccga tcgagccggc
      661 cggggggtag ggcgccacga tctgggcgca gagcttggac ggggtggaca ccacgccctg
      721 gccgttgggg ccggcgagca agatgcccag ctcgtcgcac agggccacca gctcggcctg
      781 ggcggcgatg ccgtcctccc ccgcctcgcc gtagcccgcc gacgtgacga aggcggcggt
      841 gatgcccttg gcggccgccg ctcggaggag ctccgggttc accgaggccg gcgtgcacac
      901 gaagatgagg tcgaaggtgt cgtcgggcag ctcggcgatg tcggccacgg tctcgacgcc
      961 gaggacctcg gcccgctccc ggttggtagc gccgaccgca ccctcgtagc ccgaggcgag
     1021 gacgttgtgg aggctgacga acccgaactt gccggggtgg ctggaggcgc cggcgaccac
     1081 gacgcccttc gggtcgaaca gcgcccggaa ccccgcatcg gtcggccgct tcttggctcc
     1141 tcccgctgcg ctggctggat caccgacctc gacgagggcg tcgacggcga tgggcgtgcc
     1201 gtccacgacg atgagcgggt tcaagtcggc cgagcggatc gacgcatccg cctcggcggc
     1261 ggacgacagg ccgagcagga cctgggcgag cgccgtccgg tcgaccgctg cctcgccccg
     1321 gaacgcgccg agcagtgcct gggtcccgag ctcgtcgatc atctcggcgg cgtcgacgtc
     1381 ggtgatgggc accggccgga tggcgacgtc ggcgatggcc tcggcgagga tgccgccgat
     1441 ccccaccatc acggtcatcc cgaactgcgg gtcgtcggcc agcccggcga tcagctcccg
     1501 gttgcccttc agcatcggcg cgacgaggag cccgacctcg ccgtcctctg gcgtggcggc
     1561 gtcgaggagc gagcgcgctg cggcgcgcac ggcgtcctcg tcggcgaggt cgaggtgcac
     1621 gagaccccgc tcggtcttgt gggcgatggc gtcgccgttc agcttcacca cgaccggcag
     1681 cccgacgtcg accgctgcgg tgaccgcctc ctccgccgtg gccacgaccc gctccggagc
     1741 gaacggcacc ccgtgcccag cgagcaggat cttcgagtcg gcttcgctca gcgtgcgtcc
     1801 gggcatggcg ggcgatccta ccgaccgatc ctaccgacca gccctcactg ccccgggcca
     1861 ccgggtgacg cccaataggg acgatctagg gggccgtggg cgagagggcg acgtggaaga
     1921 ggttcctcac gtgcttgagg cgggcttcga gcgccgcggg gctggtgggg tcccggtcca
     1981 ggaggctggt gaggaacggg gcctcggaga agtagccgaa catgatgctg taggccgtga
     2041 tgagcagctg ctcggtgtcg acccggcgga agtgcccagc ctcgatctcg cgctcgaacc
     2101 agcccatcgc ccgctggagg tacggccgca gcgctgcgcc caggtcgatg ccgaggttgg
     2161 cgccgtcgtc gagcgcttca cggctgatga tccggacgaa ctccgggttc tcctggaaga
     2221 agcggaagcc ggtggtgatg accagatcga ccttctgcca gccctcgacc ccgcgcccga
     2281 cggcgtcttc gacgcggatc gaccactcgg ccagggagat ccggaacacc tccgcgtaca
     2341 ccgcctcctt ggacgggaag tggtgcagca ggctgggacg gcggatgccg accgcctcgg
     2401 cgatctcgtt gagcgaggtg ccctcgtacc cgtgctcggc aaagcaccgc cgagcttcga
     2461 cgatgatcgc ttcccgcgtg gagaggaccg gttcggtggc ggccatcggt gcgatcctac
     2521 gccgtgacct gtcgcgaacg tgtggggttc cgggcatcgg cccggccggg acgggttccg
     2581 aagggcggca gagggccggt acggtccacg gaatggacat cgtggccgcc gtcttcatcg
     2641 agaacatcga gctccggcag gtggagggtc cgtcgacccg gatcgacctc accggcgtgc
     2701 acttctccct cgccgcaccc gctcccgttc cggtgacgat ggagccccac ctagtcgtgc
     2761 tcgtgcgctg cgcccccgac gaagccggga ccggcgcgct cgagaccgtg taccagcgcg
     2821 acggcgagca ggtggcccgc aacgtgcagc cgctccaggt cgaaccgggc aagttcgcct
     2881 accggctggt ccgcgctgag ctgacgttcg acgactacgg cacggtcgag gccgagatcc
     2941 gcatcgacca gggccacatc accacggtcc cgttcacgct gctgccgccc gtcgccgctg
     3001 ggagctgacg cgatgccgtt cgcggcggcg atgtcacagc acccgctcgc cacccacgcg
     3061 gtcggcgagg tcgtcgggga ggtgctcgac cgcctcggtg acgaacccga tgccgccgcc
     3121 gtcttcgtga ccagcccgtt cgccggagcg atggaggaca tcgcggcgac cgttcggacg
     3181 ctgctccggc ccgcctcgct gatcggtgcc accgccgcgt cggtgctcgc cggcgaacgc
     3241 gaggtcgagg gcgaggcagc cgtctcgctg ttcgccatgc gcttcggccc gcgtcggcgc
     3301 ttccacagcc cgccggtcac cgaggccgtg cgcatccgag ccgagcgcga agacgacggg
     3361 tggcgcgtcg aggcgtccgg cagcgccgcc ttcgccggct ccaccctcgt cttgctggcc
     3421 gatccgttct cgttccccgt ggagacgttc atcgaggacc tcgggactcg gcggccgggc
     3481 ctcacggtgg tgggcggcct ggcgtcgtcg tccaccggtc cgggcggcaa ccgcctcgtg
     3541 gccgacggcc gcgtgttcag cgacggcgcc gtcggtctcc tgctcccggc cggcgtcggc
     3601 gtcaccccgg tggtgtcgca gggctgccgc cccgtcggtg agccgctggt ggtcacgaag
     3661 gccagcggga acctcctcga ggagatcgca ggccagccgg ccttggaccg gctgatggcc
     3721 caggccgacg cggccaccac cgaggatcgc aaccgcatgg cacgagggct gcacgtgggc
     3781 ctggtggtcg acgagcgcaa ggccgagttc gatcgcggcg acttcttgat ccgatcggtc
     3841 ctgggggcgg accacgcccg caaggccgtc gccgtcggag ccgaggtgga ggtgggggcg
     3901 accgtccagt tccaggtccg cgacgcggac tccgccgacg aggacctgcg cgacctgctc
     3961 gccggcaccc ggggcgatgc cgccctgctc ttcacgtgca ccgggcgggg aacggccctg
     4021 ttcggcaccc cgcaccacga cgcccggatc gtgagccagc acgtcgacgg cggacccgtg
     4081 gccgggatgt tctgcgccgg cgaggtcggc ccgctgggcg accgcacgtt cgtgcacgtc
     4141 ctgtccgcca ccgccctgct gttcgacgac cccttctaga tcctggtgac cgcccggttg
     4201 ggctggtgga cgacgtcgcc gggctggccc agggcacggg tccaggcggc gaactccagg
     4261 aggatgccgt cggggtccaa gaagtagagc gatcggacga agacgtcgcc gttccactcg
     4321 tcggccaggc cgaactcgct gtcgtcgtgg ttggcgatct cgctggtctc cacgcctgcc
     4381 gcccgcagct tcgcctggta ctcctcgagc ctctcggccg ggacctggaa ggcgacgtgg
     4441 ttcatcgagc cgatggcact ggccagggcg ccctggtcag gccggcagcc gggaccgctc
     4501 acgccggcct ggggctcctg ggcatcgggg aaccagaaga aggcgaggca gtcaccgccg
     4561 ccgcagtcga agaagaagtg ctggcccaag ccgaccggca gttcgatcgt cttgaccagc
     4621 ggcatgccga ggatgccctc gtagaagtcg acggtggcct gcatgtcccg gcacacgagc
     4681 gccaggtggt tcaccccacc cagctcgaac ggtcgatcac ccatggtccc ctgctcctgt
     4741 cggcgatgcg ccgattctgg ctcggacgcg accgtccccg ccgagatgct cggcggggac
     4801 ggggcgctgc gtcgggcgga cccggcctag ccggcggcgg gatcgaggct gagggccttc
     4861 ttctccatga aggcatcgac gccttcgggg ccgagctcgc ggccgaggcc ggagttcttg
     4921 tagccgccga agggagcgcc gaacgccatg gcgaagtggt tgacgttgat ggtcccggtg
     4981 cggatccgct tggcgatggc cgtggcctcg tcgacatcgg cgctccacac ggaaccggac
     5041 aggccgtact gcgagtcgtt ggcgatgcgg acggcgtctt cgacgtcgtc gaacgggatg
     5101 accgacagga ccgggccgaa gatctcctcc tgggcgatgg tcatcttgtt gtcgacgtca
     5161 ccgaacacgg tgggctcgat gaaccagccc ttctcgtggc tggtgcggcc gccgccggtg
     5221 gtgacgcgag cgccctcgct gcggccggtg tcgagcaggc cgaggatgcg ctcgcgctgg
     5281 cgctcggcga cgaccgggcc cacctgggtg gcggggtcga gcggatcgcc caccaccagg
     5341 gcggcgacgg cctcggtcac ggcgtcgagc acgttcgcgt agttctcacg ggaggcgagg
     5401 atgcgggtct gggccacgca ggcctgaccg ttgttcatga tgccggcgtc gagcagctgc
     5461 gggacgatgg cggccacatc ggcgctgtcg aggaccatgg ccgccgactt gccgcccagc
     5521 tccagggtca cggggcggag gagggcgccg caggcggcac cgatcttcat gccggccgcg
     5581 gtggacccgg tgaaggtgat cttgtcgacc agcgggtggg tgacgaggta ctcgccgatc
     5641 tcgcggccgc cggggacgat gctcagcacg cccttgggca ggccggcctc ctccatgatc
     5701 tcggcgaaga cgtaggggtc gagcgccgtc tccggcgacg gcttcaggac cacggtgcag
     5761 ccggcgatga cggccggggc caccttcatg acggcctcga acagcggcac gttccacggg
     5821 atgatggcgc cgacgacgcc caccgcctc
//
LOCUS       DATMFS010000201         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_124501, whole genome shotgun sequence.
ACCESSION   DATMFS010000201 DATMFS010000000
VERSION     DATMFS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124501"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNQ33_03715"
     CDS             <1..518
                     /locus_tag="VNQ33_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97241.1"
                     /translation="YLREMAICYSDDILHRMMSMACRDPMSGIPNLEFLSVYLEELIA
                     AEVDHDPDRAGRTSLALVVVRPPGPAMVERMTSRLLVGHLIRSAVPPELIVAHVAPER
                     FVVVMPPLVAPETAVSSLRAALHGRREFEALKVTVIPLPRDRDELLGLVSSWRREAMK
                     GPPITGEGDLG"
     gene            577..1695
                     /locus_tag="VNQ33_03720"
     CDS             577..1695
                     /locus_tag="VNQ33_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWJ97242.1"
                     /translation="MSATYDASQHARQLAVELGAIVDGARFHLSRKGYDQGEVDAFLQ
                     RCADTVQRVTSLIDEGSGNAREDRAWVNFWDGGGDDSIVDDNGIVGMLGDARFHLRKK
                     GYDVGEVDELLGKLHRRATELDAAVARRLEGPPVDDHVTESHEFDLTIVDHPLVEPAD
                     PPVEREEPVPPAARRAPDEHDGPGLGRVPRSSTPVDEPRAGGVSGASEEIHDTFVAEA
                     IAEAYAAGSRQGAACSWLSEAADQRDARLDRARWWGRPSETARREADELRRAVAEECL
                     RIIGDAEREAERALVRVAEQVQQRLAKVRVEAGQELERIVAEQADRSAPAGATAEVGR
                     ASSGPPPAADSSFDEPMSPVVSLPRRPAGSPSYGRPTD"
     gene            1990..2583
                     /locus_tag="VNQ33_03725"
     CDS             1990..2583
                     /locus_tag="VNQ33_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ97243.1"
                     /translation="MAKPSIEVSDDQLVCRMRDGDRVAFEVLYERHYADVEKYVRRLC
                     PGLELVDDVVSEAFFKTLRAVANGRGPVDGFRLYIFTAARTTAIRMMGVASVEAASPM
                     MDLLLDEADGGPLEVGEGEGAAVEALRALPERAQRVLWLMEVEGWTSAEVGRAMNISA
                     NAASALAYRSRQRLRERYQKMLLELDPPAWPAAPHRS"
BASE COUNT          373 a          839 c          969 g          418 t
ORIGIN      
        1 tctacctgcg tgagatggcc atctgctaca gcgacgacat cctccatcgg atgatgtcga
       61 tggcgtgccg tgaccccatg agcgggatcc cgaacctgga gttcctctcg gtctacctcg
      121 aggagctcat cgccgcggag gtcgatcacg acccggaccg ggcaggacgc acctccctcg
      181 cgctggtggt ggtgaggcct ccgggcccgg ccatggtcga gcgcatgacc tcgcgcctgc
      241 tggtggggca cctcatccgc agtgcggtac ccccagagct gatcgtcgcc cacgtcgcgc
      301 ccgagcggtt cgtggtcgtg atgccgccgc tggtggctcc cgaaacggcg gtgagctcgc
      361 ttcgagcggc gctgcacggc cgccgcgagt tcgaggcgct gaaggtgacg gtgatcccgc
      421 tgcctcgtga ccgcgacgag ctgctgggcc tggtgagctc gtggcggcgt gaggcgatga
      481 agggtccccc catcacgggt gagggcgacc tgggttgaac cgagtggacg ggtcgatcac
      541 ccgtgcccat ccacatcagc cccaaggagc gcactgatgt ccgccaccta cgacgcgtcg
      601 cagcatgcgc gtcagcttgc cgtcgaactg ggagccatcg tcgatggcgc ccgcttccac
      661 ctctcacgca aggggtacga ccagggcgag gtggatgcct tcttgcagcg ctgtgcggac
      721 acggtccagc gggtcacctc gctcatcgac gaaggctccg ggaacgcccg ggaggaccgt
      781 gcctgggtga acttctggga cggtgggggc gacgactcga tcgttgacga caacggcatc
      841 gtcgggatgc tcggcgatgc ccggttccac cttcggaaga agggctacga cgtcggtgag
      901 gtcgacgaac tgctcggcaa actgcaccgt cgggccacgg agctcgacgc cgccgtcgcc
      961 cgtcgcctgg agggtcctcc cgtcgacgac cacgtcacgg agtcccacga gttcgacctc
     1021 acgatcgtgg accacccgct cgtcgagccg gcggatccgc cggtcgaacg ggaggagccg
     1081 gtgccaccgg ccgctcgccg tgcaccggac gagcacgatg ggcccggact cggccgcgtc
     1141 cccagatcga gcacgccggt ggatgagccc cgtgcgggcg gcgtctcggg cgcatccgag
     1201 gagatccacg acaccttcgt ggccgaggcc atcgccgagg cctacgccgc aggctcacgt
     1261 caaggtgcgg cctgcagctg gctgagcgaa gcggctgatc agcgtgatgc tcggctcgat
     1321 cgcgctcgct ggtggggccg gccgtcggag acggcccgcc gcgaggccga cgagctgcgc
     1381 cgagcggtcg ccgaggagtg cctgcggatc atcggggacg ctgagcggga ggcggagcgc
     1441 gccctggtgc gcgttgccga gcaggtgcag cagcggctgg ccaaggtccg cgtggaggcg
     1501 gggcaggagc ttgagcgcat cgtcgccgag caggccgatc ggtccgcacc ggccggtgcc
     1561 accgcagagg tgggccgggc atcgtcgggt cctccgcctg ctgctgactc gagtttcgac
     1621 gagcccatgt cgcccgtcgt ctcgctgccg cgccgtccag cgggatcgcc gtcgtacggc
     1681 cggccgacgg actgagacat gacccgccct ggccgatcgc cgccgatgag gtggcatcgc
     1741 ctggccttct cggcggtcct ggaggcaggt gggtgagacg gccgtcaggc ccgggtgagg
     1801 gcaggggtga agtgcagctc gcgccggggt gaaccaggcc tccaagcctt gtcgggcgtt
     1861 ttcgccgtcg tcatgctgct cgtggcgtgc ccagccgggt gcgcggggga tctccagcgg
     1921 catcgagcac tcgatgccgg tctggtcagg cgcgtctttt gtcgcgcctg tgcaacaggg
     1981 agggttgtga tggcgaagcc atccatcgag gtgtcggacg accagttggt ttgtcggatg
     2041 cgagacggag accgcgttgc gttcgaggtg ctctacgagc gccactacgc cgatgtcgag
     2101 aagtacgtga ggcggctgtg tcctggactg gagctggtcg acgatgtcgt gtcggaggcg
     2161 ttcttcaaga cgctgcgtgc ggtggccaac gggcgggggc ccgtggacgg gttccggctc
     2221 tacatcttca ccgccgcacg cacgacggcg atccggatga tgggggtggc gtcggtcgag
     2281 gcggcgtcac cgatgatgga tctcctcctc gacgaggccg atggcggccc gcttgaggtc
     2341 ggtgagggcg aaggcgcggc ggtcgaggcg ttgcgagcgc tgcccgagcg ggcgcagcgc
     2401 gtcttgtggc tcatggaggt cgaagggtgg accagcgcgg aggtcgggcg ggccatgaac
     2461 atctcggcca acgccgcgag cgcgttggcc taccggtccc ggcagcgctt gcgtgagcgg
     2521 taccagaaga tgctgctcga gctggatccc ccggcgtggc ctgcggctcc tcaccgttcc
     2581 tgaggcctga cggtgtgag
//
LOCUS       DATMFS010000202         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_31500, whole genome shotgun sequence.
ACCESSION   DATMFS010000202 DATMFS010000000
VERSION     DATMFS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31500"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1200)
                     /locus_tag="VNQ33_03730"
     CDS             complement(106..1200)
                     /locus_tag="VNQ33_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HWJ97244.1"
                     /translation="MRHQIFTTIGLAGLLTIGIAVPALGAPGDDGFLGTAEDFAVLAG
                     STVTNTGPTVVTGALGVSPGSAVTGFPPGTVLGVIHSGDTTADTAQTDTTTAYNDIAI
                     LPPGTDLTGQDLGGLTLPAGVYDFATTAQLTGTLTLDAAGVADSVFIIRTGSTLTTAS
                     SSTVALINGADLCNVYWQIGSSATLGTNSTFQGTILALTSITATTGAAVEGRLLARNG
                     AVTLDENVVTRPICTAAPLPTTTGLTVSPNPVTAGATTELTATVTGSTPTGTVEFFDG
                     TSSLGSATVVGGTATLSITTLTPGDHTITAVYSGDGANATSTSAPVVVTVGAALPPTG
                     LDGGAAGLIGVLLLAAGGIVLSLRRIRQAH"
     gene            complement(1402..1475)
                     /locus_tag="VNQ33_03735"
     tRNA            complement(1402..1475)
                     /locus_tag="VNQ33_03735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1439..1441),aa:Pro,seq:cgg)
     gene            complement(1512..>2485)
                     /locus_tag="VNQ33_03740"
     CDS             complement(1512..>2485)
                     /locus_tag="VNQ33_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ97245.1"
                     /translation="PAASHIDMTRVNATGTALGVVAAAGLAAFAWGALIERNRYQVRQ
                     EILPVLAAGSRTITILHLSDLHMAPWQREKQDWLARIASITEPDLVITTGDLLGHEDG
                     LSGVRRAFEPFLARRTPGAFVHGSNDYFAPRAINPFAYFAGPSRAEERGRLDTAGLET
                     FLQDQLGWFDVAEQARAIELRGSSLELIGVDDPHLGLDRLDRLPQLVEEMREVKPEDQ
                     PAVTLALAHAPYRRVLDAFTTQGADVIFAGHTHGGQVRIPGLPALVTNCDIPREQAQG
                     LSTWTHARRTAWLEVSAGIGTSIYAPVRFACPPEAVVLTLVAAPASA"
BASE COUNT          370 a          824 c          900 g          391 t
ORIGIN      
        1 gcgcggcgag ctcgtcgatc cctcccggtc gatcgagccg cagctggtgc agttgttgat
       61 ggaatagttg acgggcgctc gatcagacca gtctgactat tccgactagt gtgcctgcct
      121 gatccggcgc aagctgagca cgatgccgcc ggccgccagc agcaggacgc cgatcagccc
      181 cgcggcgcca ccatcgagtc ccgtgggtgg cagcgctgcg ccgacggtga cgacgaccgg
      241 cgcggaggtc gacgtggcgt tcgcaccatc gccggagtag acggcggtga tcgtgtgatc
      301 gccaggcgtg agcgtcgtga tcgagagcgt cgcggtgccg cccacgacgg tcgcgctgcc
      361 gagcgatgac gtcccgtcga agaactcgac cgtgccagtc ggggtgctcc ccgtgacggt
      421 ggcggtcagc tccgtcgtcg cgcccgcggt gaccgggttc gggctcacgg tcagcccggt
      481 ggtggtcggc aggggtgccg ccgtgcagat cggtcgggtc acgacgttct cgtccagggt
      541 cacggcgccg ttccttgcga gcagtcggcc ctccacggcg gccccggtgg tggcggtgat
      601 cgaggtgagc gcgaggatcg tgccctggaa ggtcgagttc gttcccagcg tcgctgagct
      661 gccgatctgc cagtagacgt tgcagaggtc cgcaccattg atgagcgcca cggtgctgct
      721 cgacgcggtg gtgagcgtcg agccggtcct gatgatgaac accgagtccg cgacgcccgc
      781 cgcatccagc gtgagggtgc cggtgagctg cgcggtcgtg gcgaagtcgt agacccccgc
      841 ggggagcgtc aggccgccga gatcctgccc ggtgagatcc gtcccgggcg ggagtatcgc
      901 gatgtcgttg tacgccgtcg tcgtgtcggt ctgcgcggtg tccgccgtcg tatcgccgga
      961 atggatgacg ccgaggacgg tcccgggtgg gaagccggtc acggcgctgc cgggtgacac
     1021 accgagtgct cccgtgacga cggtcggtcc cgtgttggtg acggtcgaac cggcgagcac
     1081 cgcgaagtcc tcggcggttc cgaggaagcc gtcatcgcct ggcgcaccga gcgcgggcac
     1141 ggcgatcccg atggtgagta gcccggcaag gccgattgtg gtgaaaatct ggtggcgcat
     1201 ttggtgcccc ctaacgggac cgatgccgca cgtccagcag gtgatcgagg ccggccgaca
     1261 ctggcccagc tgtgctcgcc tctggctaga actaccgcaa gtcaaccacg ccgcggcgga
     1321 acttgggact actggccgcc gggctcgggc cgggatgagg acgacgaagg gctgagatcc
     1381 ttatgatctc agccctgcac gtcggggtga caggatttga acctgcggcc tcttcgtccc
     1441 gaacgaagcg cgctaccaag ctgcgccaca ccccgaagga cctacagagt ctaccggatg
     1501 tcgggaccag ctcaggcgct cgcgggcgcg gcgacgagcg tcagcacgac cgcctccggc
     1561 gggcaggcga agcgcaccgg cgcgtagatc gacgtgccga ttcccgcgga cacctcgagc
     1621 caggcggtgc gccgggcgtg ggtccaggtg gagagtccct gcgcctgctc ccgagggatg
     1681 tcgcagttgg tgacgagcgc gggcagcccc gggatccgca cctggccgcc atgcgtgtgg
     1741 ccggcgaaga tgacgtccgc gccctgcgtc gtgaatgcgt cgagcacccg acgatacggc
     1801 gcgtgcgcga gggcgagggt gacggcgggc tgatcctccg gcttcacctc ccgcatctcc
     1861 tccaccagct gggggagccg gtcgagccga tcgagcccca ggtgcggatc gtcgacgccg
     1921 atcagctcga ggctcgagcc ccgcagctcg atcgcccggg cctgctcggc gacgtcgaac
     1981 caaccgagct ggtcttgtag aaacgtctcg aggcctgccg tgtcgaggcg cccgcgctcc
     2041 tcagccctgc tcggccccgc gaagtaggcg aatggattga tcgcccgcgg cgcgaagtag
     2101 tcgttcgacc cgtggacgaa ggcgcccggc gttctccgtg ccaggaacgg ctcgaatgcc
     2161 cgccgcacgc ctgaaagccc gtcctcgtgg ccgagcaggt caccggtcgt gatcaccaga
     2221 tccggctcgg tgatcgacgc gatccgggcg agccagtcct gcttctcccg ctgccacggc
     2281 gccatgtgca ggtcgctcag gtgcaggatc gtgatcgtcc gcgatccggc cgcgagcacc
     2341 ggcaggatct cctggcgcac ctggtagcgg ttgcgctcga tgagcgcccc ccacgcgaac
     2401 gcggcgagac cggccgcggc gacgacgccg agtgccgtgc cggtcgcgtt gactcttgtc
     2461 atatctatgt gggaggcggc ggggc
//
LOCUS       DATMFS010000203         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53564, whole genome shotgun sequence.
ACCESSION   DATMFS010000203 DATMFS010000000
VERSION     DATMFS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53564"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /gene="glgX"
                     /locus_tag="VNQ33_03745"
     CDS             <1..1441
                     /gene="glgX"
                     /locus_tag="VNQ33_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWJ97246.1"
                     /translation="YNTIGFFAPHADYAAHPGRQVEEFKGMVRALHQADIEVILDVVY
                     NHTGEGNHLGPTLSMRGIDNCAYYRLVEGDEQHYMDYTGTGNSLNVRHPHTLQLIMDS
                     LRYWVTEMHVDGFRFDLAATLARQFHEVDRLSAFFDIVQQDPVISQVKLIAEPWDVGP
                     GGYQVGNFPPQWSEWNGLYRDTVRDFWRGEPATLGEFASRIAGSSDLYQDNGRRPYAS
                     INFVTAHDGFTLNDLVTYNHKHNEANGEDGRDGADDNRSWNCGVEGPTDDEEVLELRR
                     RQRRNLMATLMLSQGVPMILHGDEIGRSQGGNNNVYCQDNEVSWVDWDSRAEERALLE
                     FTSALAAFRNRHPIFRRRRFFEGKPVRKADELRDIEWFTPSGDEMEEQNWEDELGKSI
                     AVFLNGDAIADRDARGIRVTDDSFVLAFNAHSEPADFTLPPEDYGQGWKVVVDSSTGE
                     VERPGAETLGAHATFTVPPRALVVMQRVG"
     gene            1441..>2395
                     /locus_tag="VNQ33_03750"
     CDS             1441..>2395
                     /locus_tag="VNQ33_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWJ97247.1"
                     /translation="MSAPSSTYRLQLRPGFGFDHARALVDQLAELGVGALYLSPVMEA
                     TPGSTHGYDVVDPARLRDELGGERGFRDLVAAAREAGLGVVVDIVPNHMSVEVPEANP
                     WWSSVLREGQASPYAHHFDIDWSRGRILVPILGSEADLSELSVEDGHLTYFDHRFPLA
                     EGTAHGTPQEVHDRQHYELVDWRRGNSELNYRRFFDITTLAAVTVERPEVFEQAHREI
                     LRWVDAGDVTGLRVDHPDGLADPGEYVQRLREAAPDAWIVIEKILHPGEDLPAGWPVD
                     GTTGYDALREIFGVLLDPAGERTFTALADRLGVPTDYAAVEE"
BASE COUNT          378 a          861 c          815 g          341 t
ORIGIN      
        1 ctacaacacg atcggcttct tcgcgccgca cgcggactac gccgcccacc cgggtcgcca
       61 ggtcgaggag ttcaagggca tggtgcgggc gctgcaccag gccgacatcg aggtcatcct
      121 cgacgtggtc tacaaccaca cgggggaggg caaccacctc ggcccgaccc tgtcgatgcg
      181 gggcatcgac aactgcgcgt actaccggct ggtcgagggc gacgagcagc actacatgga
      241 ctacaccggc acgggcaact cgctgaacgt gcgtcacccg cacacgctcc agctcatcat
      301 ggactcgctg cgctactggg tcaccgagat gcacgtggac gggttccgct tcgacctcgc
      361 ggccacgctc gcgcgccagt tccacgaggt cgaccgcctc tcggcgttct tcgacatcgt
      421 gcagcaggac ccggtcatct cccaggtcaa gctcatcgcc gagccctggg acgtcggacc
      481 cggcgggtac caggtcggca acttcccgcc gcagtggtcg gagtggaacg gcctctaccg
      541 cgacaccgtg cgcgacttct ggcgcggtga gccggcgacg ctgggcgagt tcgcctcccg
      601 gatcgcgggc tcgtccgacc tctaccagga caacggtcgg cggccctacg cctcgatcaa
      661 cttcgtcacg gcgcacgacg ggttcacgct caacgacctc gtcacctaca accacaagca
      721 caacgaggcg aacggtgagg acggccgcga cggcgcggac gacaaccggt cgtggaactg
      781 cggcgtggag ggcccgacgg acgacgagga ggtgctggag ctgcgccgcc gccagcgccg
      841 caacctcatg gccaccctga tgctgtccca gggcgtgccg atgatcctgc acggcgacga
      901 gatcggtcgc tcgcagggcg gcaacaacaa cgtctactgc caggacaacg aggtcagctg
      961 ggtcgactgg gactcgcgtg ccgaggagcg cgctctcctc gagttcacct cggccctggc
     1021 ggccttccgc aaccgtcacc cgatcttccg gcgccgccgc ttcttcgagg gcaagccggt
     1081 ccgcaaggcc gacgagctgc gcgacatcga gtggttcacc ccctccgggg acgagatgga
     1141 ggagcagaac tgggaggacg agctcggcaa gtccatcgcg gtgttcctga acggtgacgc
     1201 gatcgccgac cgtgacgccc gggggatccg ggtcaccgac gactcgttcg tgctggcgtt
     1261 caacgcccac agcgagccgg cggacttcac gctcccgccg gaggactacg gccagggctg
     1321 gaaggtggtc gtcgacagct cgaccggcga ggtcgagagg ccgggcgccg agacgctcgg
     1381 cgcccacgcc acgttcaccg tcccgccccg agcgctcgtc gtgatgcaac gggtcggctg
     1441 atgtccgctc cgtcgtcgac gtaccggctg cagctgcggc ccgggttcgg cttcgaccac
     1501 gcacgcgcgc tggtggacca gctcgcggag ctcggcgtgg gcgcgctcta cctgtccccg
     1561 gtgatggagg cgacgcccgg ctcgacccac ggctacgacg tcgtcgatcc cgcacgactg
     1621 cgcgacgagc tcggcggcga gcgcggcttc cgcgacctcg tggcggccgc gcgcgaggcg
     1681 ggcctgggcg tcgtcgtcga catcgtgccg aaccacatgt ccgtcgaggt gcccgaggcg
     1741 aacccgtggt ggtccagcgt gctgcgcgag gggcaggcct ccccgtacgc ccaccatttc
     1801 gacatcgact ggtcccgggg acgcatcctc gtgccgatcc tcgggagcga ggccgacctg
     1861 tccgagctgt cggtcgagga cggccatctg acctacttcg accaccgctt cccgctggcc
     1921 gagggcacgg ctcacggcac cccgcaggag gtgcacgatc ggcagcacta cgagctggtc
     1981 gactggcgcc gcggcaacag cgagctcaac taccggcggt tcttcgacat cacgaccttg
     2041 gccgccgtga ccgtcgagcg gcccgaggtg ttcgagcagg cgcaccgcga gatcctccgc
     2101 tgggtcgatg ccggggacgt gacgggcctg cgggtcgacc atcccgacgg gctcgccgac
     2161 cccggcgagt acgtgcagcg actgcgggag gccgcgcccg acgcctggat cgtgatcgag
     2221 aagatcctgc acccgggtga ggacctgccc gccggctggc cggtcgacgg caccaccggc
     2281 tacgacgccc tccgtgagat cttcggcgtg ttgctcgacc ccgccgggga gcgcacgttc
     2341 acggcgctcg ccgaccggct cggcgtcccc accgactacg ccgcggtcga ggagg
//
LOCUS       DATMFS010000204         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_262420, whole genome shotgun sequence.
ACCESSION   DATMFS010000204 DATMFS010000000
VERSION     DATMFS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262420"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VNQ33_03755"
     CDS             complement(<1..182)
                     /locus_tag="VNQ33_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWJ97248.1"
                     /translation="MAVIPARLQPVLDEVRPVAEALVGAGHRAYLVGGIVRDQLLAKP
                     LAPESDIDLTTDATPP"
     gene            complement(419..1501)
                     /locus_tag="VNQ33_03760"
     CDS             complement(419..1501)
                     /locus_tag="VNQ33_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWJ97249.1"
                     /translation="MTQVVELLQYLLEERGSDLLVKVGSPPCLRRNGKLERTPLETLG
                     PEVIESLAAELLTPIKAAEFQETGETDLAHSVPGLGRFRVNVYRQRGSVSLAIRRVVP
                     GAPAIADLGVPQVVADLASSRDGLVIISGPAASGKTTTAAAMIDHINASQSRHIVTIE
                     DPIEVLHADRMAIVSQREVGADTADVIDALRRVARQDADVIFISDLIDPLAVSEALSV
                     AAAGRLVIGVMNTLSVIDTLSWLIDRQEQHAQEHVRQLLARVLRGIICQRLVDRSDGR
                     GRVAVVESVTATPKVRDAIASGADPTLIERLMSEGDYHGMATFDQALFNLFSDDLISL
                     DEALDRAAHPEDFRIAVQQAGLATLR"
     gene            complement(1534..2565)
                     /locus_tag="VNQ33_03765"
     CDS             complement(1534..2565)
                     /locus_tag="VNQ33_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWJ97250.1"
                     /translation="MPVLYATHPQYLEHSAGRGHPESPARLRAVQLGVQRAGLAEALV
                     AVEPVAAGDAELERIHPEAYRRALRSFCASGGGQLDGDTAVVPESWDAALLAAGAGLE
                     VIRRLQSGDGDAGFCAVRPPGHHALASQAMGFCLFNNVAVAAAQLRSEGERVAIVDYD
                     AHHGNGTQDLFWDDPDVFYVSTHEWPQYPGTGGIREVGVGEGVGTTLNLPFPSHTTGD
                     VYRRAFDEVVIPALAAWQPTWLLISAGFDAHRADPLTDLGLTSADFADVTVALLSLVP
                     PGRRLLFLEGGYDLDALSLCTGSVLSALADDGAYRPEAATSGGPGGDVCAAALKIRGE
                     LADGYLRSP"
     gene            2684..3403
                     /locus_tag="VNQ33_03770"
     CDS             2684..3403
                     /locus_tag="VNQ33_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97251.1"
                     /translation="MAADDRWWGARAHLQVSAWRGSDHTVVMSPVPDHPAPGPADIQR
                     ELDVLADRGVRRVLTGALHHGEVAPFRAAGFVEHERLHLLRHDLRDLPESTTPVRLRR
                     AWRRDQHDVLTIDERAFDEFWALDRGGLDDAVRATPASRFRIAASDRATTGYAITGRA
                     ADRGYLQRLAVDPDRHREGIGRALVADSLRWLRRTGARVAVVNTQEHNAGALGLYLAT
                     GFALEPHGLTVLSCALGSPPR"
     gene            3400..>3607
                     /locus_tag="VNQ33_03775"
     CDS             3400..>3607
                     /locus_tag="VNQ33_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97252.1"
                     /translation="MIRRIVTAGLLTASLLVVAPTPSHAQSAPVASGPRLTLVDQPIS
                     VAPDGSFSVVLDVTDAPTATQIAVD"
BASE COUNT          501 a         1328 c         1314 g          464 t
ORIGIN      
        1 tgcggcggcg tggcgtcggt ggtgaggtcg atgtcggact cgggcgcgag cggcttggcg
       61 agcagctggt cccgcacgat cccgcccacc aggtacgcgc ggtggcccgc gccgaccagg
      121 gcctcggcca ccggccggac ctcatcgagc acaggctgca gccgagcggg gatcacggcc
      181 accaaggtac gggacgcctc cgaaccggca ggagtccgtt gccggccgga cagcccgccg
      241 acgacgtgcg atccgccttc ggtcaggcgg cgcgaccgcc ggcgtcagct cggatttcgg
      301 gtcacaaagg cacctctgcg gtgcacgagt gacccgaaat cggagccgcg accgaggacg
      361 ggtgcgatgc ggcgccggtc gcgcggggac gaacccggcc ggttgcggga cctgcctcct
      421 agcgcagcgt cgcgaggccg gcctgctgga cggcgatgcg gaagtcctcg gggtgggcgg
      481 ctcggtccag ggcctcgtcg aggctgatca ggtcgtcact gaacaggttg aacagggcct
      541 ggtcgaacgt cgccatgccg tggtagtcgc cctcgctcat caggcgctcg atcagggtcg
      601 ggtcggcgcc cgaggcgatg gcgtcgcgca ccttcggggt cgcggtgacc gactcgacca
      661 cggcgacgcg gccccggccg tcggagcggt ccacgaggcg ctggcagatg atgccgcgca
      721 gcacccgggc gaggagctgg cggacgtgct cttgggcgtg ctgctcctgg cggtcgatca
      781 gccagctgag ggtgtcgatc accgacaggg tgttcatcac gccgatgacc aaccggccgg
      841 cggcggcgac cgacagcgcc tcgctgacgg cgagggggtc gatgaggtcg ctgatgaaga
      901 tgacgtcggc atcttggcgg gcgacgcgcc gcagggcgtc gatgacgtcg gcggtgtcgg
      961 ccccgacctc gcgctggctg acgatcgcca tgcggtccgc gtggaggacc tcgatcggat
     1021 cctcgatggt gacgatgtgg cgcgactgcg aggcgttgat gtggtcgatc atcgcggcgg
     1081 cggtcgtcgt cttcccggag gcggccggcc ccgagatgat cacgaggccg tcgcggctgc
     1141 tggccagatc ggccaccacc tgcggcacgc cgaggtcggc gatcgccggg gcaccgggca
     1201 ccacgcgccg gatcgccagg ctcaccgagc cgcgctggcg gtacacgttg acgcggaagc
     1261 gaccgaggcc ggggacgctg tgggccaggt ccgtctcccc ggtctcttgg aactccgccg
     1321 ccttgatggg ggtgaggagc tccgcggcga ggctctcgat gacctcggga ccaagggtct
     1381 cgagcggggt ccgctcgagc ttgccgttcc gacggaggca cggcggcgac cccaccttca
     1441 cgagcaggtc cgagccccgc tcctcgagca ggtactgcag cagttcgacg acctgcgtca
     1501 cggcaaccct ccggggtcag gacaggcgcc tcctcatgga gaccgcaggt atccgtcggc
     1561 cagctcaccc cggatcttga gggctgcggc gcagacatcg ccgcccgggc cgccgctcgt
     1621 ggcggcctcg ggacggtagg cgccgtcgtc ggccagagcc gacagcaccg aacccgtgca
     1681 caaggagagg gcgtcgaggt cgtagccccc ctcgaggaac agcaggcgac ggcccggcgg
     1741 caccagcgag agcagggcca cggtcacgtc ggcgaagtcg gcggaggtca gaccgagatc
     1801 ggtgaggggg tcggcgcggt gggcgtcgaa gccggccgag atcagcagcc acgtcggctg
     1861 ccacgccgcc agggccggga tgaccacctc gtcgaacgcg cgccggtaga cgtcgccggt
     1921 ggtgtggctc gggaacggca ggttgagggt ggtaccgacc ccctcgccga ccccgacctc
     1981 ccggatgcca ccggtcccgg ggtactgcgg ccactcgtgg gtggacacgt agaagacgtc
     2041 ggggtcatcc cagaacaggt cctgggtccc gttgccgtgg tgggcgtcgt agtccacgat
     2101 cgccacccgc tccccctcgc tgcgcagctg cgcggcagcc accgccacgt tgttgaagag
     2161 gcagaagccc atcgcctggc tggcgagcgc gtggtggccc ggcggccgca ccgcgcagaa
     2221 cccagcgtca ccgtcgccgg actggagccg ccggatcacc tcgaggccgg cgcccgcggc
     2281 cagcagggcg gcgtcccagg attcgggcac caccgcggtg tcgccgtcga gctgcccgcc
     2341 gccggacgcg cagaacgacc gcagcgcgcg gcggtaggcc tcggggtgga tccgctcgag
     2401 ctccgcgtca ccggcggcga ccggctccac ggcgaccaac gcctcggcga gcccggcccg
     2461 ctgcaccccg agctgcaccg cacgcagccg cgccggcgac tcgggatggc cgcggccggc
     2521 cgagtgctcg aggtactgcg ggtgggtcgc gtacaggacc ggcatcgctc cagggtacgg
     2581 gcgccggggc gccgcggccc tcagccgacg agccgggtcg cggccccgta ccacctaagg
     2641 tccggaccag tgctcgacac ctcaccaccc cgggcctggc gtgatggctg ccgatgatcg
     2701 gtggtggggc gcacgcgccc acctccaggt gtcggcctgg cgcggcagcg accacacggt
     2761 ggtgatgagc ccggtccccg atcaccccgc cccgggaccg gccgacatcc agcgcgagct
     2821 cgacgtcctc gcggatcgtg gcgtccggcg ggtgctcacc ggcgccttgc accacggtga
     2881 ggtggcaccg ttccgcgccg ccggcttcgt cgagcacgaa cggctgcacc tcctgcgcca
     2941 cgacctgcgc gacctgcccg agtcgacgac gcccgtccgc ctccgccgag cctggcggcg
     3001 cgatcagcat gacgtcttga cgatcgacga gcgggcgttc gacgagttct gggccctcga
     3061 ccgcggcggc ctcgacgacg ccgtgcgcgc cacgccggcc agccgcttcc gcatcgccgc
     3121 gtcggaccgg gccaccaccg gctacgccat caccggacga gccgcggacc gcggctacct
     3181 gcagcgcctg gccgtcgacc ccgaccgcca ccgcgagggg atcggccggg ccctcgtggc
     3241 cgacagcctt cgctggctgc gtcgcaccgg cgcccgcgtc gcggtcgtca acacgcaaga
     3301 gcacaacgcc ggtgcgctcg ggctctacct ggccaccggc ttcgcgctcg agccccacgg
     3361 cctgaccgtg ctcagctgcg cgctcggaag cccgccccgg tgatccgccg catcgtcacg
     3421 gcaggcctgc tcaccgcctc gctgctggtc gtcgctccga cccccagcca cgcccagagc
     3481 gccccggtag cgtccgggcc tcgcctgacg ctcgtcgacc agccgatctc cgtggccccc
     3541 gacggcagct tctcggtcgt gctcgacgtg accgacgcac ccacggccac ccagatcgcc
     3601 gtcgaca
//
LOCUS       DATMFS010000205         5004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_168758, whole genome shotgun sequence.
ACCESSION   DATMFS010000205 DATMFS010000000
VERSION     DATMFS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168758"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..1190
                     /locus_tag="VNQ33_03780"
     CDS             429..1190
                     /locus_tag="VNQ33_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97253.1"
                     /translation="MALLDVRDIEVRFGGVVALDGLSFDVAAGEICALIGPNGAGKTT
                     MFNVVSRLYEAQAGHVTFDGQDLLALPPHKIAKIGVARTFQNLALFPALTVLENVMVG
                     AHIEGKVGFISSMLRIGSATEEKRLRSECREILDRMSLGHLADRPAFGLPFGTLKRLE
                     FARALASRPKLLLLDEPANGLTHGEVDELGDTIKHIRDEFDLTILLVEHHMSMVMAIS
                     HMVVVMDFGRKIAEGTPDQVRDDPVVIEAYLGTPA"
     gene            1190..1897
                     /locus_tag="VNQ33_03785"
     CDS             1190..1897
                     /locus_tag="VNQ33_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97254.1"
                     /translation="MLLELRSVSGGYGPVNVLHGLDLGVTEGEVGVILGANGAGKTTT
                     LRAICGMLDDISGEVFFDDRDITRDSPQATARRGVAHVPQGRGTFGDLSVDENLRLGA
                     ITRKDGGVRSDLDRWYEAFPRLAERRTQAAGSMSGGEQQMLAIARALMSRPRLLLLDE
                     PSLGLAPLITRDLFARLGEINRTEGLTMLIVEQNAGLALEIAQRGYVLETGSIVAQGT
                     AEELAADEDVRKAYLGF"
     gene            1897..2808
                     /locus_tag="VNQ33_03790"
     CDS             1897..2808
                     /locus_tag="VNQ33_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ97255.1"
                     /translation="MDALNDSLFVTQFLNGIGLGAVYASVALALVIIYRTTGLLNFAQ
                     GEMALFSTFVTWWLTERGVAIWPALLGSMVFSFFAGAAIERLIIRPVEKSKNQLNLVI
                     VTLGLFLAINAVCQLLFIKPGQDQVLMPLMFPGGKLNLIVIDIPWAIVGRAAVLAVEC
                     AVLYLLLQKTKIGLALRAVASNPESSRLVGINPGTMLMLGWGIAAVLGAVAGTMAASR
                     GLGGFDTSLMQQVLVYAFAAAALGGFDSPLGAVVGGLIVGVARTLTVQYIGWLEGIDL
                     IVPLGLIFVVLLVRPNGLFGRNIVERV"
     gene            2808..3911
                     /locus_tag="VNQ33_03795"
     CDS             2808..3911
                     /locus_tag="VNQ33_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ97256.1"
                     /translation="MSDRSTNLPDTPPAFATPSAPVVAGEKRLPWLRIVFGVVVAVVA
                     LALPYLNDPSTNQIFCQVMYLALAAMGLNLLTGYNGQISLGHGAFFGVGAFTTAILVV
                     DHGWMIEATFPVAALLAALVGVAVGFPALRVKGLYLALITLGLAVLFPLVVKKFVHGS
                     GGVALLKPKRSQVSSVVDFWADDIYRYYVCLAILVVLFILAWNLVRGRMGRAMIAVRD
                     QEVAASTVGIGVANVKVWSFALSAAYAGLAGALSVYIDRTADATNPIVYFQRSIEFLI
                     AVVIGGAATIGGPLLGAMLLVAIRRRTEGTEAMAPALLGGALILVIYVLPDGIVGGLR
                     RLSTWIRGRTSGGAPPPDGAPEPVAPAPAPSPT"
     gene            3934..>5004
                     /locus_tag="VNQ33_03800"
     CDS             3934..>5004
                     /locus_tag="VNQ33_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ97257.1"
                     /translation="MHTKRTTKQRRPLTALLLLAVLALLATSCGRSEDTGKGGGSDGG
                     GGGNGDGESGAVVDTADCPDTGTAGIDGDTIKLASSFPQSGLTSAFAQISKGYLAYFD
                     KVNAEGGVEVAGKKYKIEVTAKDDEYKPASTAQNIESEVGTDGTDAFAVFNVVGTANN
                     VAIRDDLNDLCVPNIFAATGSPVWGNESYPWTIGSTLAPYTVEAKAFADYLKENKPDA
                     KVAMLVQNDDFGKAYEEGFKQQIEGSDITVTQVKTYEAGTDEVTAEVTSLKASGADTF
                     FNGATLLACPNALNAADEQGWDAVTFVSGTCISKTLMGIAGEAADGVFATTNIKDPLN
                     PAFANDSAMKEYNETLKEYGAKD"
BASE COUNT          754 a         1805 c         1639 g          806 t
ORIGIN      
        1 gccgaggaga cggagatccc cgtcaccctc gtggaccagc cgctcgaggc cgtggtcctc
       61 ggcgccggcc gctgcatcga ggcctacgac cagctccgcg tgatgttcat gggcgcccgc
      121 ggctgacggc ttcccggtcc gccgaggcgc cgcagaacgt ggaggatcct ggtcgctcag
      181 cgacaacgat cctccacgtt ccgccacgga cctccaagtt ggaggatctg ggtcacagag
      241 cgccatcacc ctccaagttg gaggaacatg gtcggggggg ggagcgggct gacgggtcct
      301 ggcggcttgc gcctgtcacg agaacacgtt ccggtccagg tatctcctgg tcctgactag
      361 ggtgaccgcc accacgcggg tgtggcccgc cgtggcggtc caggggacga acaaggggtt
      421 gccgggtctt ggccctgctc gacgtacgtg acatcgaggt gcggttcggc ggggtggtgg
      481 ccctcgacgg cctgagcttc gatgtcgccg ccggggagat ctgcgccctc atcggcccga
      541 acggcgccgg caagaccacc atgttcaacg tggtgagccg cctctacgag gcccaggccg
      601 gccacgtgac cttcgacggg caggacctgc tggcgctgcc gccccacaag atcgccaaga
      661 tcggtgtggc ccgcaccttc cagaacctgg cgctgttccc ggcgctcacg gtgctggaga
      721 acgtcatggt cggcgcccac atcgagggca aggtcgggtt catctcgtcg atgctgcgga
      781 tcggctcggc caccgaggag aagcggctcc gctcggagtg ccgcgagatc ctcgatcgca
      841 tgtcgctcgg ccacctggcc gaccggccgg cgttcggcct gcccttcggc acgctcaagc
      901 ggctcgagtt cgcccgcgcc ctggcctcac gccccaagct gctgctcctc gacgagccgg
      961 ccaacggcct cacccacggc gaggtcgacg agctcggcga caccatcaag cacatccgcg
     1021 acgagttcga cctcacgatc ctgctggtgg agcaccacat gtcgatggtc atggccatct
     1081 cgcacatggt cgtggtcatg gacttcggcc ggaagatcgc cgagggcaca ccggaccagg
     1141 tgcgcgacga cccggtcgtc atcgaggcct acctggggac gccggcctga tgctcctcga
     1201 actgcgatcc gtctccggcg gctacggccc ggtgaacgtc ctgcacgggc tcgacctcgg
     1261 ggtcaccgag ggcgaggtcg gggtcatcct cggggccaac ggcgctggca agaccaccac
     1321 gctgcgggcc atctgcggga tgctcgacga catctcgggc gaggtgttct tcgacgaccg
     1381 cgacatcacc cgggacagcc cgcaggccac ggcgcgcagg ggagtcgccc acgtcccgca
     1441 aggacggggc accttcggcg acctctcggt cgacgagaac ctgcggctcg gcgccatcac
     1501 ccgcaaagac ggcggcgtcc ggtccgacct ggaccgctgg tacgaggcct ttccccgcct
     1561 ggccgagcga cgcacgcagg ccgcggggtc gatgtcgggc ggtgagcagc agatgctcgc
     1621 catcgcccga gccctcatga gccggccgcg gctcctgctg ctcgatgagc cgtcgctcgg
     1681 cctggcgccg ctcatcaccc gtgacctgtt cgcacgcctc ggcgagatca accgcaccga
     1741 agggctgacc atgctgatcg tggagcagaa cgccgggctg gcgctcgaga tcgcccagcg
     1801 cggctacgtc ctcgagacgg ggagcatcgt cgcccagggc accgccgagg agctcgcggc
     1861 cgacgaagac gtccgcaagg cgtacctggg gttctgatgg acgcgctcaa cgacagcctc
     1921 ttcgtcaccc agttcctgaa cggcatcggc ctgggcgcgg tgtacgcgtc ggtcgccctc
     1981 gccctcgtga tcatctaccg caccacgggc ctgctgaact tcgcccaggg cgagatggcc
     2041 ctgttctcca ccttcgtcac ctggtggctc accgagcgcg gtgtggccat ctggccggcg
     2101 ctgctcggct cgatggtgtt ctcgttcttc gccggtgccg ccatcgagcg gctcatcatc
     2161 cggccggtcg agaagtcgaa gaaccagctc aacctcgtga tcgtcacgct gggcctgttc
     2221 ctcgccatca acgccgtctg ccagctgctc ttcatcaagc ccggtcagga ccaggtcctg
     2281 atgcccctga tgttcccggg cggcaagctg aacctgatcg tcatcgacat cccgtgggcc
     2341 atcgtcggcc gagccgccgt gctcgccgtc gagtgcgcgg tcctgtacct gctgctgcag
     2401 aagaccaaga tcgggctggc gctgcgggcc gtcgcctcga accccgagtc gtcccgcctc
     2461 gtcggcatca atccgggcac catgctcatg ctcgggtggg gcatcgctgc tgtgctgggc
     2521 gccgtcgccg gcacgatggc ggccagccgc gggctcggcg ggttcgacac ctcgctgatg
     2581 cagcaggtgc tggtctacgc cttcgccgcg gcggcgctcg gcgggttcga cagcccgctc
     2641 ggcgcggtgg tgggcggttt gatcgtgggc gtcgcccgga cgctgaccgt gcagtacatc
     2701 gggtggctcg aggggatcga cctgatcgtt cccctcggct tgatcttcgt cgtgctgctg
     2761 gtgcggccga acggcctgtt cggccgcaac atcgtggagc gggtctgatg agcgatcggt
     2821 ccaccaacct tcccgacacg cctccggcct tcgccacccc gtcggctccg gtcgtcgccg
     2881 gcgagaagcg gctgccttgg ctgcggatcg tgttcggtgt cgtcgtcgcg gtggtggcgc
     2941 tggccctgcc gtacctcaac gatccgtcca cgaaccagat cttctgccag gtgatgtacc
     3001 tcgccctggc ggccatgggc ctcaacctgc tcaccggcta caacggccag atctcgctgg
     3061 ggcacggcgc gttcttcggt gtcggcgcct tcaccaccgc catcctcgtg gtcgaccacg
     3121 gctggatgat cgaggcgacg ttccccgtcg ccgccctgct ggctgcgctg gtcggagtcg
     3181 ccgtcggctt cccggccctc cgggtcaagg ggctctacct ggcgctcatc accctcggcc
     3241 tcgccgtgct cttcccgctc gtggtgaaga agttcgtgca tggctctggt ggcgtggccc
     3301 tgctcaagcc caagcgctcc caggtgtcgt ccgtggtcga cttctgggcc gatgacatct
     3361 accgctacta cgtgtgcctg gcgatcctcg tggtgctctt catcctggcg tggaacctcg
     3421 tgcggggccg catgggccgg gcgatgatcg ccgtgcgcga ccaggaggtg gccgcttcga
     3481 cggtgggcat cggcgtggcc aacgtcaagg tctggtcgtt cgccctctcg gccgcctacg
     3541 cggggcttgc cggtgcgctg tcggtgtaca tcgaccgcac cgccgacgcc accaacccga
     3601 tcgtctactt ccagcgctcc atcgagttcc tgatcgcggt ggtcatcggc ggcgccgcca
     3661 ccatcggcgg cccgttgctc ggagccatgc tgctggtcgc catccgccgc cgcaccgagg
     3721 gcaccgaggc catggccccc gccctgctcg gcggcgcgct gatcctcgtc atctacgtgc
     3781 tgcccgacgg catcgtcggg ggcctccgcc gcctcagcac ctggatccgg ggccgcacgt
     3841 ccgggggcgc acctccaccg gatggcgcgc ccgagccggt ggcgcccgca cccgcacctt
     3901 cgcccacctg acccccaacg aggagacact cccatgcaca ccaagcgcac gaccaagcag
     3961 cggcggccct tgaccgcact gctcctgctc gccgtcctcg ccctgctggc cacctcctgc
     4021 ggccgcagcg aggacaccgg caagggcgga ggttccgacg gcggtggtgg aggcaacggc
     4081 gacggcgagt ccggcgccgt cgtcgacacc gccgactgcc ccgacaccgg caccgccggc
     4141 atcgacggcg acaccatcaa gctggcctcg tcgttcccgc agtcgggcct gacgtcggcg
     4201 ttcgcgcaga tctcgaaggg ctacctcgcc tacttcgaca aggtgaacgc ggaaggcggc
     4261 gtcgaggtgg ccggcaagaa gtacaagatc gaggtcaccg ccaaagacga cgagtacaag
     4321 ccggcctcca ccgcccagaa catcgagtcc gaggtcggca ccgacggcac cgacgccttc
     4381 gccgtcttca acgtggtcgg cacggccaac aacgtcgcca tccgtgacga cctcaacgac
     4441 ctgtgcgtgc cgaacatctt cgccgccacc ggctcgccgg tctggggcaa cgagagctac
     4501 ccgtggacca tcggctccac actggctccc tacaccgtcg aggccaaggc gttcgccgac
     4561 tacctcaagg agaacaagcc cgacgccaag gtggcgatgc tcgtccagaa cgacgacttc
     4621 gggaaggcct acgaagaggg cttcaagcag cagatcgagg gctcggacat caccgtgacc
     4681 caggtcaaga cctacgaggc cggcaccgac gaggtcaccg ccgaggtcac gtcgctgaag
     4741 gcctccggcg ccgacacctt cttcaacggg gccaccttgc tggcctgccc gaacgccctc
     4801 aacgcggccg acgagcaggg ctgggacgcc gtcaccttcg tgtcgggcac ctgcatctcc
     4861 aagaccctga tgggcatcgc cggcgaggcc gccgacgggg tgttcgccac cacgaacatc
     4921 aaggacccgc tgaaccccgc cttcgccaac gactcggcga tgaaggagta caacgagacc
     4981 ctgaaggagt acggcgccaa ggac
//
LOCUS       DATMFS010000206         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_333858, whole genome shotgun sequence.
ACCESSION   DATMFS010000206 DATMFS010000000
VERSION     DATMFS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333858"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VNQ33_03805"
     CDS             complement(<1..333)
                     /locus_tag="VNQ33_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97258.1"
                     /translation="MSDGTADPTTVADAFEGAHGYRPFDADNHYYEALDAFSRHLDPK
                     HKARVFDWAVMGRRTYPVLGGKVFRGVKNATFDPVAFPGVLADYFRGNPNDEDPLELL
                     SRHEPIRHE"
     gene            complement(382..1206)
                     /locus_tag="VNQ33_03810"
     CDS             complement(382..1206)
                     /locus_tag="VNQ33_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ97259.1"
                     /translation="MRVPSTDGVELELHDFGGDGPTLLIAHATGFCAGAYLPMVPLLA
                     EQFHVRGIDFRAHGDSTAPDDLSWRGMADDVLAVVDALGGDPILGFGHSMGGASLVAA
                     ELLRPGTLERAFVFEPIVIPAAWNDRGPGQNPMSTAARRRRPTFASRDEVLGRYARRP
                     PLGVFRADALAAYVDHGFTDAPDGTVTLKCTPEHEALTFEAPNKPTIEQVHEVTTPLV
                     VARGERDGELGPAAFSGLIAAAAPNAELRRYERLSHFGPFEDPDLLAREAVAFLRN"
     gene            complement(1218..1616)
                     /locus_tag="VNQ33_03815"
     CDS             complement(1218..1616)
                     /locus_tag="VNQ33_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWJ97260.1"
                     /translation="MATRRQVDKALREVRAACLALPEVIERPSHSAPTFFIRGKKSFV
                     MFHDDHHGDGILGIWCAAPPGVQEELVEAEHERFYRPAYVGHRGWLGVRLDVAPDWDE
                     LRGIVTDAYRQVAPKALVAQLDSEQAGAAE"
     gene            complement(1620..>2134)
                     /locus_tag="VNQ33_03820"
     CDS             complement(1620..>2134)
                     /locus_tag="VNQ33_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97261.1"
                     /translation="IIGQEYRFNTIRPTFTAAPWRLRVLVAKLAVVTSATAVVALVMV
                     AACGLIGALMLSPFTIDGTDQRVVWGTVLFCAGWTMLGMGLGAIIRQPIAAMLVLLVE
                     AFVVENIIVAISEGAAKWMPFVNGIQMTVRDPDESGMFRSPLNGGIYFFVIATVIWVI
                     GAVLATKRDA"
BASE COUNT          315 a          701 c          807 g          311 t
ORIGIN      
        1 ctcgtggcgg atgggctcgt ggcggctgag cagctcgagc gggtcctcgt cgttcgggtt
       61 gccccggaag tagtcggcga ggacgcccgg gaaggcgacc ggatcgaagg tggcgttctt
      121 cacgcctcgg aagaccttgc cgccgaggac cgggtaggtg cgccggccca tcaccgccca
      181 gtcgaagacc cgggccttgt gcttgggatc gaggtggcgg ctgaacgcgt cgagcgcctc
      241 gtagtagtgg ttgtcggcgt cgaagggccg gtagccgtgg gcgccttcga aggcgtcggc
      301 gacggtcgtg gggtcggcgg ttccatcaga cactggctcg ctccctggcg ctggccgtac
      361 cccctcaggc tagaacgtgt ttcagtttct gaggaaggcg acggcctcgc gggccagcag
      421 gtcggggtct tcgaagggcc cgaagtggct gaggcgctcg tagcggcgca gctcagcgtt
      481 cggtgctgcg gcggcgatga ggcccgagaa ggccgccgga ccgagctccc cgtcgcgctc
      541 gccgcgggcg accaccaggg gggtggtgac ctcgtggacc tgctcgatgg tgggcttgtt
      601 cggcgcctcg aacgtgaggg cctcgtgctc gggcgtgcac ttcagggtga ccgtgccgtc
      661 gggagcatcg gtgaacccgt ggtcgacgta cgcggccagg gcatcggcgc ggaacacgcc
      721 gagcgggggc cggcgggcgt agcggcccag cacctcgtcg cgggaggcga acgtcggccg
      781 gcgtcggcgg gcggcggttg acatcgggtt ctggccgggg ccgcggtcgt tccacgccgc
      841 cgggatgacg atcggctcga acacgaacgc ccgctcgagc gtgccgggcc gcaggagctc
      901 ggcggccacg aggctggcgc cgcccatgga gtggccgaag ccgaggatcg ggtcgccgcc
      961 gagcgcatcg accacggcga ggacgtcgtc ggccatgccc cgccacgaca ggtcgtcggg
     1021 tgctgtcgag tcgccgtgcg cccggaagtc gatgccccgc acgtggaact gctcggccag
     1081 cagcgggacc atcgggaggt aggcgccggc gcagaagccg gtggcgtggg cgatcagcag
     1141 ggtggggccg tcgccgccga agtcgtgcag ctcgagctcg acgccgtcgg tggaggggac
     1201 gcgcatggga gctggtgcta ctcggcggcg ccggcctgct ccgaatcgag ctgggcgacg
     1261 agcgccttgg gcgccacctg gcggtaggcg tcggtgacga tgccgcgcag ctcgtcccag
     1321 tcgggtgcca cgtcgaggcg cacgccgagc cagccccggt gcccgacgta ggcggggcgg
     1381 tagaagcgct cgtgctcggc ctcgaccagc tcctcttgca ccccgggcgg cgccgcgcac
     1441 cagatgccga ggatcccgtc accgtggtgg tcgtcgtgga acatcacgaa cgacttcttg
     1501 ccgcggatga agaacgtcgg tgcgctgtgg gacggccgct cgatcacctc gggcagcgcg
     1561 aggcaggcgg cccgcacctc tcggagggcc ttgtcgacct gccgtcgggt cgccatcgcc
     1621 tacgcgtccc gcttggtggc gaggacggcg ccgatgaccc agatcacggt ggcgatgacg
     1681 aagaagtaga tgccgccgtt cagcggagac cggaacatcc cgctttcatc gggatcacgg
     1741 accgtcatct ggatgccgtt cacgaacggc atccacttcg ccgcaccctc ggagatcgcc
     1801 acgatgatgt tctcgaccac gaacgcctcc accagcaaga cgagcatggc ggcgatcggc
     1861 tgccggatga tggccccgag ccccatgccg agcatcgtcc atccggcgca gaacagcacg
     1921 gtgccccaca cgacgcgctg gtcggtgccg tcgatcgtga acggactgag catcagcgcg
     1981 ccgatgagac cgcaggccgc caccatgacg agcgccacca cggcggttgc gctcgtgacg
     2041 acggccagct tcgcgaccag gacgcggagg cgccaagggg ccgccgtgaa cgtcggtcgg
     2101 atggtgttga accggtactc ctgcccgatg atct
//
LOCUS       DATMFS010000207         3446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53610, whole genome shotgun sequence.
ACCESSION   DATMFS010000207 DATMFS010000000
VERSION     DATMFS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53610"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VNQ33_03825"
     CDS             <1..625
                     /locus_tag="VNQ33_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HWJ97262.1"
                     /translation="LLDTKGMTLEAVTEAFAAHPDAAVVRLHSGDPAWYSAIGEQIAW
                     CVAHERSFEVVPGVSSVAAAAAALGAELTVPGVSQTVVLTRLAKRTTRSLGPGDDLAA
                     YAAQGGLLALFLSAGDPERLQEVLLGPGSAYSSATPAAIVHRVSWPDEQVITTTVGGI
                     AAALIERGITTTALVLVGDALGARTASCERSHVYDPSFATAFRDATG"
     gene            622..1806
                     /gene="cbiE"
                     /locus_tag="VNQ33_03830"
     CDS             622..1806
                     /gene="cbiE"
                     /locus_tag="VNQ33_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HWJ97263.1"
                     /translation="MSASIAVVGLHGGEAFGRAAAEALAGAGVVAAAPRHLQQLDLAA
                     AQEHIELSGPLHAVLDQVAARLDDGRSVAVVASGDPGFFGIVRMLGERFGAVRLVVHP
                     APSSVALAFGRIGTNWDDPIVVSAHGRPIDAAVEAATRYPKVAVLTAPQHPPELLGRA
                     LVAAGCPARQVAVATRLGEPDEAVTRTDLDGLAAGAFDPLSVVVLLDLNPDDAAETLA
                     WGLPEQEFAHRGGLITKSEVRAVALGKLALPRHGVLWDVGAGSGSVGIECARLSPRLR
                     VVAVERNEDDAERIRSNAKRHGVAVEVVVGSAPEALAGLPTPDRVFVGGGGIDVVRTA
                     FDALRPGGTLVATHVLVDRAAAAWELLGDTVQVATSRAVPIADGFRLDAQNPVFVSWG
                     TR"
     gene            1803..2225
                     /locus_tag="VNQ33_03835"
     CDS             1803..2225
                     /locus_tag="VNQ33_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HWJ97264.1"
                     /translation="MTDVVVGLGLTRDASIDDVRAAIDDALSEIDCGWSDVVRLATTV
                     RRRGHPAIDAVTADGTIPLSLFEPSDLARVTVPNPSAAVGARAGTTSVAEAAALMAAN
                     AEILLLPKQTGKGVTVALAEHALPHPSAALRAGSRGPG"
     gene            2598..3035
                     /locus_tag="VNQ33_03840"
     CDS             2598..3035
                     /locus_tag="VNQ33_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97265.1"
                     /translation="MERSKGQSTVHTQDRPATRPESAAPELDGLRMGRRHRKLLGIGR
                     LPADTMRPAGPQCLPRAVETLEDLDAPGRHRRELLVELRQFHPEHVSLGLELEPILPG
                     NALDLPERQVLTVALPLLALGSRSRREDDVLIVAVHRAQASGS"
     gene            complement(3243..>3446)
                     /locus_tag="VNQ33_03845"
     CDS             complement(3243..>3446)
                     /locus_tag="VNQ33_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97266.1"
                     /translation="GLFEHDAFRWQFLRELAERRGKHFEPSGVVFAEARAARFDRIAD
                     AIEHHLDLAALAALIAEASDAPA"
BASE COUNT          431 a         1271 c         1243 g          501 t
ORIGIN      
        1 cctcctcgac accaagggca tgaccctcga agcggtgacc gaggcgttcg ccgcccaccc
       61 cgacgccgcc gtcgtgcggc tccactcggg cgacccggcc tggtactccg ccatcggcga
      121 gcagatcgcc tggtgcgtcg cccacgagcg ctcgttcgag gtggtgccgg gcgtgtcatc
      181 tgtagccgcg gcggccgccg cgctcggcgc ggagctgacc gtgcccggcg tgtcgcagac
      241 cgtcgtgctg acccgcctcg ccaagcggac cacgaggtcc ctcggccccg gcgacgacct
      301 cgccgcctac gcggcccagg gcgggctgct ggccctgttc ctctcggccg gcgacccgga
      361 gcggctccag gaggtgctgc tcggacccgg ctcggcctac tcgtcggcaa cgccggcggc
      421 gatcgtgcac cgggtgagct ggcccgacga gcaggtgatc accacgacgg tcggcgggat
      481 cgccgccgcc ctcatcgagc ggggcatcac cacgaccgcg ctcgtcttgg tgggcgacgc
      541 gctcggcgct cgcacggcct cgtgcgaacg cagccacgtc tacgacccgt cgttcgccac
      601 cgcgttccgc gacgccaccg gatgagcgcg tcgatcgcgg tcgtcggcct gcacggcggt
      661 gaggcgttcg gccgggccgc cgccgaggcc ctggccggcg ccggggtcgt cgccgccgcc
      721 cctcgccatc tccagcagct ggacctggcc gcggcccagg agcacatcga gctgtccggt
      781 ccgctccacg ccgtgctcga ccaggtggcg gcgcggctcg atgacgggcg ttcggtcgcc
      841 gtcgtcgcat cgggcgaccc ggggttcttc gggatcgtgc ggatgctggg cgagcggttc
      901 ggggccgtgc gcctcgtcgt ccatccggcg ccgtcatcgg tggcgctcgc gttcgggcgc
      961 atcggcacca actgggacga ccccatcgtg gtgtcggccc acggccgtcc catcgacgcc
     1021 gccgtcgaag ccgcgacccg ctaccccaag gtcgccgtgc tcaccgcgcc ccagcacccg
     1081 cccgagctgc tgggaagggc gctggtggcc gccgggtgcc cggcgcgcca ggtggccgtc
     1141 gccacccgcc tgggcgaacc ggacgaggcc gtcacccgca ccgacctcga cggcctggcg
     1201 gcgggtgcgt tcgacccgct gtccgtcgtg gtgctcctcg atctgaaccc cgacgatgcg
     1261 gccgagaccc tggcctgggg cctgcccgag caggagttcg cccaccgggg cggcctcatc
     1321 acgaagtccg aggtgcgggc ggtggcgctc gggaagctcg ccctgccccg ccacggcgtg
     1381 ctgtgggacg tgggcgccgg cagcggatcg gtgggcatcg agtgcgcccg gctctcacct
     1441 cggctgcggg tcgtcgccgt cgagcgcaac gaggacgatg ccgagcgcat ccggtccaac
     1501 gccaagcgcc acggcgtcgc cgtcgaggtg gtcgtcggct ccgctcccga ggcgctggcg
     1561 ggcctgccga ccccggaccg ggtgttcgtc ggcggcgggg ggatcgacgt cgtgcggact
     1621 gccttcgacg cgctccgacc cggcgggacc ctcgttgcca cccacgtgct ggtcgaccgg
     1681 gcagcagccg cctgggagct gctgggcgac accgtgcagg tcgccacgtc acgggccgtc
     1741 ccgatcgccg acggattccg cctcgacgcc cagaaccccg tgttcgtcag ctgggggacg
     1801 aggtgaccga cgtcgtggtc ggcctgggcc tgaccaggga cgcatcgatc gacgacgtcc
     1861 gcgccgccat cgacgacgcc ctgtccgaga tcgactgcgg ctggagcgac gtggtccgcc
     1921 tggcgaccac cgtccgccgc cgcggccacc ccgccatcga tgccgtcacc gctgacggca
     1981 ccatcccact ctcgttgttc gaaccgagcg acctggcccg ggtgacggtc cccaacccgt
     2041 ccgccgccgt cggcgcccga gcgggcacca ccagcgtcgc cgaggccgcc gccttgatgg
     2101 ccgccaacgc cgagatcctc ctccttccca agcagaccgg gaagggcgtc acagtcgccc
     2161 tcgccgaaca cgccctgccc catccttcgg ctgctcttcg agcggggagc cgcgggccgg
     2221 gttgactccg ccgcctggcc gtccgccgga cgggcccgat cggccaggcc atcgtgccct
     2281 ggggccgaaa cgcttggttc cgttgggtcg tacgggctgt ccgcgctgtc cgggctgtct
     2341 gctggccacg ctgcgcccgc ggacgctgac tcgggggcac caggccatgc ctcgggatcg
     2401 agcgcttgtg cagggtcgat gaggtcgggc tccgtatcgg tctgccagcg gctcagcgga
     2461 gcgtcgggat cgatctcgta gacgccacga gcgacgcggt gcacgcgccc tcgctccacc
     2521 tcgtagccca tcgcgtcggc aatcgccttg gtgggcgtgg ggctgtacac cgcgtatccg
     2581 tagtggtgca ggagggcatg gagcgctcga agggacagtc gaccgtgcac acgcaggatc
     2641 gcccggcaac acgcccggag tccgcggccc cagagctcga tggcctccgg atgggcaggc
     2701 ggcatcggaa gctcctcggc atcgggaggc tgccggccga cacgatgagg ccagcggggc
     2761 cacaatgcct ccctcgcgcc gtcgagaccc tggaggatct cgatgcgccg ggtcgtcatc
     2821 gtcgagagct gctggtcgag ctccgtcagt tccatcctga gcacgtttcg ctcggtctgg
     2881 agcttgagcc gatcctgccg gggaacgcgc tggatcttcc ggagcggcag gttctcacgg
     2941 tagcgctgcc actgctcgcg cttggatccc gatctcgtcg ggaggatgac gtcctgatcg
     3001 tcgcggtgca tcgagcgcag gcctccgggt cgtagcggtg ggaggaggga gaccctcgag
     3061 ggaggccgtt gaccggcgag tcgccactac cttgcccggg gggtgtgaca ctccgacggc
     3121 gacgtcatac cgccgggagt cggcggcgcc ccgcaacctc ccctccctgc gtcccagcgt
     3181 gtacgcggcg aatcgacgtc gattcgccgc gtacggactc gagactcgga gcgggggcgg
     3241 ggtcaggcgg gtgcgtcgga ggcctcggcg atgagggcgg cgagggcggc gaggtcgagg
     3301 tggtgctcga tggcgtcggc gatgcggtcg aagcgggcgg ctcgtgcctc ggcgaacacg
     3361 accccgctcg gttcgaagtg cttgcccctc cgctcagcca actccctgag gaactgccag
     3421 cggaaggcat cgtgctcgaa gaggcc
//
LOCUS       DATMFS010000208         6635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_77488, whole genome shotgun sequence.
ACCESSION   DATMFS010000208 DATMFS010000000
VERSION     DATMFS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77488"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..938
                     /locus_tag="VNQ33_03850"
     CDS             42..938
                     /locus_tag="VNQ33_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWJ97267.1"
                     /translation="MAMNGNYVFRETAANLTRNITLTVASVLTVFISLSIVGATVLVR
                     QGAQNLTRQFENGVEFIVYVEPDASSSQLAEVRTSLEDNPGIAEASYIDRDETYADFK
                     QLFKGQDTMLENVRASDLPTSFRVRPKDSSVATVQGLVNYYRKQPNVFEVRAATDVIK
                     QMKGMTDFVNKLLFFSAGFLLAASTLLILNTIRTAMFARRREIEVMKLVGATNWFIRV
                     PFMLEGLVQGIIGGLLAIGAVFFFRGWIDDLLNKGQGLELLQSFTINRGDVVLACLWV
                     GITAIVLSVGSSLIATRRFLDV"
     gene            1130..1729
                     /locus_tag="VNQ33_03855"
     CDS             1130..1729
                     /locus_tag="VNQ33_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HWJ97268.1"
                     /translation="MGGTGRGPLPPAGYEAFFAAQHAPLARLAYALTGSRSDAQDLAQ
                     EVLVRSFERWSTVGAYASPEAWARRVLVNLVYSRTRRRRTEAIGLQRLRPIAAPRDGT
                     ELSDAAEAFWAAVRTLPPRQAQAVALRYGADLPAVEVAQILDVDPGTVRAHLHAARAR
                     LSAVLHVDADDQPSPTAASATRNPPADPTGHAKEHDDDV"
     gene            1719..2780
                     /locus_tag="VNQ33_03860"
     CDS             1719..2780
                     /locus_tag="VNQ33_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97269.1"
                     /translation="MTFDEWTTEAHRSLQAWNPEPAVRPAAAMARSEAGGRSAADPVA
                     TSAYAELAPARGRPPASTRRRLLAAVAAAAIVAAALAAMAVPRAGTDVAVGPQTAPTR
                     PAARPSEAVGAPSFECAAADPERGASTDVFRDATDVAGWRAGNVDDLLSPGGAQPSPN
                     EPVNHATGTLAESPGEDRAETTTRAAAFVAAVAPGWTCTVWSPEVDAADRSRPGPIVD
                     EHNVVTNRLGGMCFVPDASADPDSVWGVDPSLAAALDQVDGLTAWNVILADGGGNRQD
                     PGRRWLEFTFEADPGAGDSGSTTRVLAAVSAAVDAAGAAAGVPTECSPTERSGPEHPQ
                     LRSAPPPGPGVARRGVRIG"
     gene            complement(2727..3569)
                     /locus_tag="VNQ33_03865"
     CDS             complement(2727..3569)
                     /locus_tag="VNQ33_03865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HWJ97270.1"
                     /translation="MLRPAGIDAATAVLAWAGFLAPEAEAAELAAAADDDAHLAAMVQ
                     RRTTGEPLAWITGTTTFCGETLRVDPGVYVPRPQTEPLARAAAGVLPRDGTAVDLCTG
                     SGALAVVLARQHPDARVIGTDLDPAAVRCARANGVEAYEGDLAAAAPSEVHGQVDLVT
                     AVVPYVPSEALHLLPRDVTAHEPRRALDGGTHGTAILVRAVEAAAGLLRPGGHLVLEL
                     GGDQDRRLGATLARLGFTGIRPHHDEDGDLRRLEARSPTHPGRRRSPDPNATSGDTGS
                     WRRG"
     gene            complement(3594..4841)
                     /locus_tag="VNQ33_03870"
     CDS             complement(3594..4841)
                     /locus_tag="VNQ33_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ97271.1"
                     /translation="MSDQAPTTVIGLNPFEPGFYDDPYEQYAAIREADPISQTALGPW
                     LITRWADVHTLLRKPGTSVEERNIEMDGPSRRQQMNERRAEQGAPPIERSLAILNIDP
                     PDHTRIRKLVAKAFTPRAVERIRTTARQQVDAILDDLDGRGEPIDLISELAFPLPFAV
                     INEMLGMPEGGDTLQLRAWSHTITQVLDPLLAMAHPDEIIAASVAMRGVIAEAIAWKR
                     TRDDDDILTAMIQAEEDGEQLTDAELLENVMLLFLAGHETTVNLIGNGVNALLDHPDQ
                     LGRLIGDPSLDANAVEELLRFDSPVQFSRRIALEPLSIEGVDIAPGELVMTCLGAANR
                     DPAKFGPSAAELRLDRPDAREHVSFGSGVHHCLGAALARLEGQEAIGRLVRRFPDMER
                     AGTPTHNQRIVLRGYDDLPISLS"
     gene            4892..6079
                     /locus_tag="VNQ33_03875"
     CDS             4892..6079
                     /locus_tag="VNQ33_03875"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWJ97272.1"
                     /translation="MVDRTMRGAAAVVGVADAVSPTGELQGTVRSLEVQVIREALDDA
                     GLTIADVDGLFTCTTPGWAASLELAEYLGIAPRWTDSTQTGGSSFEIHVQHAAAAISL
                     GLCDVAVVVYAATPRSSFKKGSGGYTSRQSLPGPTPSAEWEQPYAMRMPMGAYALAAS
                     RHMHEFGTTAEQLAQIAVDTRTWATMNPRARLQDPITVDDVLASPMQASPLHKLDCCL
                     VTDGAGAVVLTSTERARSLRKPPVLVLGTGTCHTHAMISEMPDLTTTGAAVSGPLAFA
                     QAGLTPADIDVAQIYDSFTITVLLLLEDLGFCPKGEGGAFVAGGVLAPGGALPANTSG
                     GGLAYTHPGMFGIFLLVEAVRQLRGECGPRQVDGAEVALAHGSGGVLSAMSTTILGTE
                     ATA"
     gene            6119..6523
                     /locus_tag="VNQ33_03880"
     CDS             6119..6523
                     /locus_tag="VNQ33_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWJ97273.1"
                     /translation="MASRPRCRRPPVRSGRPLGPVPSSSSGAGPASRRSTSREACPSC
                     LGADLEFRPAAGTGTVYAMSEMPAPGNAGMQGRAPYVVALVDLPEGVRMLTGIVRADG
                     GLGAAEVAVGDAVRVAWEPLADGRHLPVFVRA"
BASE COUNT          873 a         2329 c         2444 g          989 t
ORIGIN      
        1 aggcccgcgg cgtctacgag tagggcagcg aggctcgaac catggccatg aacggcaact
       61 acgtcttccg cgagaccgcg gcgaacctga cccggaacat cacgctcacg gtcgcgtccg
      121 tcctcacggt gttcatctcc ctctcgatcg tcggggccac cgtcctggtc cgccagggcg
      181 cgcagaacct gacccgccag ttcgagaacg gcgtcgagtt catcgtgtac gtcgagcccg
      241 acgcgtcgag ctcccagctc gccgaggtcc gcacgtcgct ggaggacaac cccggcatcg
      301 ccgaggccag ctacatcgac cgcgacgaga cctacgccga cttcaagcag ctgttcaagg
      361 gccaggacac gatgctcgag aacgtgcggg cgtcggatct gcccaccagc ttccgggtgc
      421 ggcccaagga ctccagcgtc gccacggtcc aagggctcgt gaactactac cgcaagcagc
      481 cgaacgtgtt cgaggtacga gcggccaccg acgtgatcaa gcagatgaag ggcatgaccg
      541 acttcgtcaa caagctgctc ttcttctccg ccggcttcct gctcgccgcc tcgacgctgc
      601 tcatcctcaa caccatccgc acggcgatgt tcgcccgccg acgggagatc gaggtgatga
      661 agctggtcgg tgccaccaac tggttcatcc gcgtcccgtt catgctcgaa gggctggtcc
      721 aaggcatcat cggcggcctc ttggccatcg gcgccgtgtt cttcttccgg gggtggatcg
      781 acgacctgct caacaagggc caggggctcg agctcctgca gtcgttcacc atcaaccgcg
      841 gcgacgtcgt gctcgcctgt ctctgggtcg gcatcaccgc gatcgtgctc agcgtgggca
      901 gctcgctcat cgccacccgt cgcttcctcg acgtctagcc ctgttccggt cggcctcctc
      961 gccgccggac cagcgtcttg ggcgctgcgg tgggagcgtg gcggcgcttc gtgggccgtt
     1021 tcccgcctgc gaggtgaacg gtgccttcga acgttctcat gccgactcaa cgtttcgggc
     1081 ttgtcggacg tgagtgatgt cgatgcgttc gacagcggat accacctcga tgggaggcac
     1141 cggccgcggg ccgttgccgc cagccggcta tgaggcgttc ttcgcggccc agcacgcgcc
     1201 cctggctcgc ttggcgtacg cgctgacggg cagtcgctcc gacgctcagg acctcgccca
     1261 ggaggtgctg gtgcggtcgt tcgagcgctg gtcgaccgtt ggcgcctacg ccagtcccga
     1321 ggcatgggcc cgacgggtgc tggtcaacct ggtgtacagc cggacgcggc ggcgacggac
     1381 ggaggcgatc ggcctccagc ggctccggcc gatcgccgcc ccgcgggacg gcacggagct
     1441 gtccgacgcg gccgaggcgt tctgggcggc ggtacggacg ctgccgcctc gacaggccca
     1501 ggcagtggcc ttgcgctacg gcgctgacct gcccgcggtc gaggtcgccc agatcctcga
     1561 cgtcgacccc ggcaccgtcc gagcacatct gcacgcggca cgagcacggc tgtccgccgt
     1621 gctgcacgtg gacgcggatg accaaccgtc gccgaccgcc gcgtcggcga ctcggaaccc
     1681 accggccgat ccgaccggcc acgcgaagga gcatgacgat gacgtttgac gagtggacga
     1741 ccgaggccca ccggagcctc caggcgtgga acccggaacc ggcggtccga cccgccgcgg
     1801 cgatggcccg atccgaggcc ggcggccggt ccgcagcaga tccggttgcg acgtcggcgt
     1861 acgccgagct cgctcctgct cgcggccgac cgcccgcatc gacgcggcga cggctgctgg
     1921 ctgcggtggc cgccgccgcc atcgtcgccg ccgcgctcgc cgcgatggcg gtcccccggg
     1981 ctgggaccga cgtggcggtc ggtccgcaga ccgcgcccac ccggcccgct gcccggccat
     2041 cggaggcggt cggtgcgccg tcgttcgagt gcgctgcggc tgacccggag cgcggcgcgt
     2101 cgaccgacgt gttccgcgac gccacggacg tcgcggggtg gcgggccggc aacgtcgacg
     2161 acctcctgtc ccccggcggg gcgcaaccct cgccgaacga accggtgaac cacgcgacgg
     2221 gcaccctggc cgagtcgccg ggtgaggacc gggcggagac gacgacacga gccgcggcgt
     2281 tcgtggcggc ggtcgctccg ggctggacgt gcaccgtgtg gtcgccggag gtcgatgccg
     2341 ccgaccgctc ccggccgggt ccgatcgtcg acgagcacaa cgtcgtcacc aaccggctgg
     2401 gcgggatgtg cttcgtgccg gacgcgtcgg cggatccgga ctccgtgtgg ggcgtcgacc
     2461 cgagcctcgc cgcggcgctg gaccaggtcg acggcctcac ggcctggaac gtgatcctcg
     2521 ccgacggagg cggcaaccgc caggacccgg gccggcggtg gctggagttc accttcgagg
     2581 ccgacccggg cgccggcgac agcgggtcca ccacgcgggt cctggccgcg gtcagcgcgg
     2641 cggtcgacgc ggcgggtgcg gccgccggcg tgcccaccga gtgctcgccc actgaacgca
     2701 gcggcccgga gcatcctcag ctgaggtcag cccctcctcc aggacccggt gtcgccagac
     2761 gtggcgttcg gatcgggtga tcgtcgccgc ccggggtggg tgggcgatcg ggcctcgagg
     2821 cgacggaggt cgccgtcttc gtcgtggtgc gggcggatgc cggtgaagcc gaggcgggcc
     2881 aacgtcgcac cgaggcgccg gtcttggtcg ccaccgagct cgaggacgag gtggccgcca
     2941 ggtcggagga ggccggcggc ggcttccacg gctcgcacca ggatcgccgt gccgtgcgtg
     3001 ccgccgtcga gcgctcgccg gggttcgtgg gcggtcacgt cgcgggggag gaggtggagg
     3061 gcctcggacg ggacgtaggg gacgaccgcg gtgacgaggt cgacctggcc gtgcacctcg
     3121 gacggcgcgg ccgcggcgag atcgccctcg taggcctcca cgccgttggc gcgagcgcag
     3181 cgcacggccg ccgggtcgag gtcggtgccg atcacgcggg cgtcggggtg ctggcgggcg
     3241 agcaccacgg caagggcacc gctgccggtg cacaggtcga cggcggtgcc gtcacgaggc
     3301 agcacgccag ccgccgctcg ggccaggggc tcggtctggg ggcgggggac gtagacgccg
     3361 gggtcgacgc gcagggtctc gccgcagaac gtcgtggtgc ccgtgatcca ggccagcggc
     3421 tcgcctgtcg tccgccgctg caccatcgcc gcgaggtggg cgtcgtcgtc cgctgcggcg
     3481 gccagttcgg cggcttcagc ctcgggcgcg aggaacccgg cccaggccaa gacggcggtg
     3541 gcggcatcga tgccggcggg gcggagcacg ccgcccaccc ttgccgacga tcgctacgag
     3601 agcgagatgg gcaggtcgtc gtagccgcgg aggacgatcc gctggttgtg ggtcggcgtg
     3661 ccggcccgct ccatgtcggg gaagcggcgg acgaggcgtc cgatggcctc ctggccctcg
     3721 aggcgggcga gggcggcgcc gaggcagtgg tgcacgcctg agccgaacga gacgtgctcg
     3781 cgggcatcgg gccggtccag ccgcagctcg gcggcgctgg ggccgaactt ggccgggtcg
     3841 cggttggcgg caccgaggca ggtcatcacc agctcgccgg gcgcgatgtc gacgccctcg
     3901 atcgagagcg gttcgagggc gatgcgccgg gagaactgga cggggctgtc gaagcggagc
     3961 agctcctcga cggcgttggc gtcgagcgac gggtcgccga tgaggcggcc gagctggtcg
     4021 gggtggtcga ggagggcgtt cacgccgttg ccgatgaggt tcaccgtggt ctcgtggccg
     4081 gcgaggaaga ggagcatcac gttctcgagc agctcggcgt cggtgagctg ctcgccgtcc
     4141 tcttcggcct ggatcatggc ggtgaggatg tcgtcgtcgt ctcgggtccg cttccaggcg
     4201 atggcctcgg cgatcacgcc gcgcatggcg accgatgcgg cgatgatctc gtcggggtgc
     4261 gccatggcga gcagcggatc gagcacctgg gtgatggtgt gggaccaggc ccggagctgc
     4321 agcgtgtcgc cgccctcggg catgccgagc atctcgttga tgacggcgaa ggggaggggg
     4381 aacgccagct cgctgatgag gtcgatgggc tcgccgcgcc cgtcgaggtc gtcgaggatg
     4441 gcatcgacct gctggcgggc ggtggtgcgg atgcgctcga cggcgcgggg cgtgaacgcc
     4501 ttggccacga gcttgcggat gcgggtgtgg tcgggcgggt cgatgttgag gatggccagg
     4561 ctgcgctcga tgggcggcgc tccctgctcg gcgcgccgct cgttcatctg ctggcggcgc
     4621 gacgggccgt ccatctcgat gttgcgctcc tcgacgctgg tgccgggctt gcgcaggagc
     4681 gtgtggacgt cggcccagcg ggtgatcagc cacggcccga gcgccgtctg gctgatgggg
     4741 tccgcctcgc ggatggccgc gtactgttcg tacgggtcgt cgtagaagcc cggctcgaac
     4801 gggttcagcc cgatcacggt cgtcggtgcc tggtcgctca tgacccgacg ataccgtgtt
     4861 cccagcagtt gttgcagtag gagtgcaaag agtggtcgat cgaacgatgc ggggcgccgc
     4921 agccgtcgtc ggggtggctg atgcggtgtc gcccaccggt gagctccagg ggacggtgcg
     4981 gtcgctcgag gtccaggtca tccgcgaggc gctcgacgat gccggcctga ccatcgccga
     5041 tgtcgacggc ctgttcacct gcaccactcc aggttgggcc gcatcgctgg agctcgccga
     5101 gtacctcggc atcgcgcccc gctggaccga ctccacccag accggcgggt ccagcttcga
     5161 gatccacgtg cagcacgccg ccgccgcgat cagcctcggc ctgtgcgacg tggcggtggt
     5221 ggtctacgcc gccaccccgc ggtcctcgtt caagaagggg tcgggcggct acacgagccg
     5281 ccagtcgctc cccggtccga ccccttcggc cgagtgggag cagccctacg ccatgcgcat
     5341 gccgatgggt gcctacgcgc tggccgccag ccggcacatg cacgagttcg gcacgaccgc
     5401 ggagcagttg gcccagatcg ccgtcgacac ccggacgtgg gcgacgatga acccacgggc
     5461 tcgcctccag gacccgatca ccgtcgacga cgtgctcgcg tcgccgatgc aggcgtcgcc
     5521 gctgcacaag ctcgactgct gcctcgtcac cgacggcgcc ggtgccgtgg tgctcaccag
     5581 caccgagcgg gcccgctcgc tgcgcaagcc gcccgtgctc gtgctgggaa cgggcacctg
     5641 ccacacccac gccatgatca gcgagatgcc cgacctcacc accaccggtg cggcggtgtc
     5701 gggtccgctg gcgttcgccc aggccggtct cacccccgcc gacatcgacg tcgcccagat
     5761 ctacgactcg ttcaccatca ccgtgctgct gctgctcgag gacctcggct tctgccccaa
     5821 gggtgaaggc ggtgccttcg tcgccggtgg cgtgctcgcc cccggcggcg cgctgccggc
     5881 caacaccagc ggcggcggcc ttgcctacac gcatccgggc atgttcggga tcttcctctt
     5941 ggtcgaggcg gtgcggcagc tgcgagggga gtgcggaccc cgccaggtcg acggcgccga
     6001 ggtggccctc gcccacggca gcggcggtgt gctctcggcc atgtccacga cgatcctcgg
     6061 cacggaggcc acggcatgac cgagcggctc gtcatcccgg cccccgaggg cgcccccgat
     6121 cgccagccgc ccccggtgtc gccggccgcc ggtccgttct gggaggccac tcggtcctgt
     6181 tccctcgtca tccagtggtg ccggtcctgc gagcaggcga tccacttccc gcgaggcctg
     6241 cccctcgtgc ctcggtgccg atctcgagtt ccggcccgcc gccgggaccg gcaccgtcta
     6301 cgccatgagc gagatgccgg cgcccggcaa cgcgggcatg cagggccggg cgccctacgt
     6361 ggtggcgctg gtcgacctgc ccgaaggcgt ccgcatgctg accggcatcg tgcgcgccga
     6421 tggcggcctg ggcgccgccg aggtggcggt cggcgatgcc gtccgcgtcg cctgggagcc
     6481 gctggccgac ggccgccacc ttccggtctt cgtgcgcgct tgaggctcga gagcagccgt
     6541 tgcgccgtgg acttcgccgg acttcgttgg aatcccgcga ggtctggcac aatggttcgt
     6601 ccccatcggt cgcccccgac ccctggaact ggagt
//
LOCUS       DATMFS010000209         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_168810, whole genome shotgun sequence.
ACCESSION   DATMFS010000209 DATMFS010000000
VERSION     DATMFS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168810"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VNQ33_03885"
     CDS             <1..807
                     /locus_tag="VNQ33_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ97274.1"
                     /translation="IVVSPYLFWTSFAGGLVAPERTIVRPCLHDEPEARFELFEPLVT
                     GVAKLWLNTDPEAELLDEIWPAHAPAEVIGEGIPVPEAYDPAGFRKRHGLSDRPFALY
                     AGRREGAKNWDALLDGFVAAIRKEDLDLDLVTFGVGEVRVPPDVEGRVVDLGLLSDRD
                     KDDAFAAAAAYLQPSAMESFSRTVMEAWLAGTPVIANAASAVVSWHCDRSGAGLTYED
                     RTELEQCLRFVAEAPTEAAALAAPGRQYVLDRYTWPVTLDRMEASLEAMP"
     gene            804..2297
                     /locus_tag="VNQ33_03890"
     CDS             804..2297
                     /locus_tag="VNQ33_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97275.1"
                     /translation="MSTPSPASASQRILVVSPYPPVRDGIGAYAVQQVRALRRQGHHV
                     EVVSPWPSAAHHHLSLEGPKGARALKKLMAGFDRVIVHFHPDVFYANPSTPTSRISTG
                     TALASAFRSGPPVEIRLHEVDRRWASATEQVEASSRSGFVATQVFEQAARASRAVFKA
                     ADEITVHVPEHERMMVDQFGVAPGRVRLVDHGEDFTRRVPDDQAVARASLGLPADGHV
                     FVCIGFIQPHKGFDRAATAFRGLGDKGASLHVVGSVRVDDPAALDHQRELEALESQIP
                     GVHLHLGFVSDEAFDRWIVAADTVVLPYRHIWSSSVAERARLYDRPVIATRVGGLADQ
                     IGEHPANTVVDDNAGLARAMAAAVGAVGADGDAARSAAPTWTFTGPIDRDTVMAEITR
                     RAAAQRGVRLKPVTAQPGAAAARSSAPAHVATPLRRLGLNPPPPPTSARPGVSTVKRL
                     IRRVIAWEVDPLREQLVDLQRAAVKAAETADARLDEVADDQGGSPSL"
     gene            complement(2451..3095)
                     /locus_tag="VNQ33_03895"
     CDS             complement(2451..3095)
                     /locus_tag="VNQ33_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97276.1"
                     /translation="MTAPANERPKSIGLAPGLDDAAAGQDAAKYGTSNPVVQKLLARW
                     MATMHTVLGDTSGVVVDIGIGEGFALERMFPADTPAVGLEYRHDKALVASEKLPEVAV
                     VRGDAGVLPFPDRSADLVTSIEVLEHLPGYEQAVVEMARICRGRLVVSVPWEPWFRLG
                     NLGRGKNVERWGNDPEHVNFFSPRKLRTALDGHFGDVKVTKAFPWIIAEATNPR"
     gene            complement(3092..4084)
                     /locus_tag="VNQ33_03900"
     CDS             complement(3092..4084)
                     /locus_tag="VNQ33_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ97277.1"
                     /translation="MDWESRQGHQLPLSRCLNEGPAVGCDVSVLMPAYNEAENLVEVI
                     PATAAALDALGRSWEIVIVDDGSTDGTRSVMSGLRSANVRYIRLRRNSGKSAALEVGL
                     NHVQGEYVVLMDADGQDDPDELPKMFAKLDEGYDLVTGRREVRNDRFIKRNTSKVYNG
                     ATSMVTGVKMKDFNSGYKLMTRELADSLEMYGELHRYIPVLAVWNGFKVTEVTVEHHE
                     RLHGTSKFGPARFWRGFLDLVTVKFLTTYTARPFHLFGGIGFGIGFGGAAILLWMFAS
                     KLLGHNIGQRPVLQLGVLLIVVAVQMISLGLLGELMVNLRRRDRLDSSAEGDLR"
     gene            4083..>4377
                     /locus_tag="VNQ33_03905"
     CDS             4083..>4377
                     /locus_tag="VNQ33_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWJ97278.1"
                     /translation="MSPGKVLITGAQGFVGRHLTTHLEACGDEVTGVDRLPDPSGTND
                     GVDITDAGAIRELIASIGPRTIYHLAGWADVGGSWKAPVEAFRANAEGTLNVLS"
BASE COUNT          614 a         1647 c         1494 g          622 t
ORIGIN      
        1 atcgtcgtgt cgccgtacct gttctggacg tcgttcgccg ggggcctggt cgcaccggag
       61 cgcaccatcg tgcggccgtg cctccacgac gagcccgagg cccgcttcga gctgttcgag
      121 ccgctggtca ccggcgtcgc caagctctgg ctgaacacgg atcccgaggc cgagctgctc
      181 gatgagatct ggccggccca cgcgccggcc gaggtcatcg gcgagggcat cccggtgccc
      241 gaggcctacg acccggccgg tttccggaag cgccacggcc tgagcgaccg gccgttcgcc
      301 ctctacgccg ggcgccgcga gggggccaag aactgggatg ccctgctcga cggcttcgtg
      361 gccgccatcc gcaaggagga cctcgacctc gacctcgtca cgttcggcgt gggcgaggtc
      421 cgcgtgccgc ccgacgtcga ggggcgcgtc gtcgacctcg gcctgctgtc ggaccgggac
      481 aaagacgacg ccttcgccgc cgccgccgcc tacctgcagc cgtcggccat ggagtcgttc
      541 tcccgcacgg tgatggaggc ctggctcgcc ggcaccccgg tcatcgccaa cgccgccagc
      601 gccgtcgtct cctggcactg cgaccgcagc ggcgccggtc tcacctacga ggaccgcacc
      661 gagctcgagc agtgcctgcg gttcgtggcc gaggcaccca ccgaggccgc cgccctcgct
      721 gctcccgggc gccagtacgt gctcgaccgc tacacctggc cggtcacgct cgaccgcatg
      781 gaagcgagcc tggaggcgat gccgtgagca cgccctcgcc cgcgtccgcc agccagcgga
      841 tcctcgtcgt ctcgccctac ccgcccgtgc gcgacggcat cggggcctac gccgtgcagc
      901 aggtccgggc gctgcgccgc cagggccacc acgtcgaggt cgtgtcgccg tggccgtcgg
      961 cggcccacca ccacctctcg ctcgagggtc cgaagggcgc ccgggccctg aagaagctga
     1021 tggccgggtt cgaccgcgtc atcgtccact tccaccccga cgtcttctac gccaacccgt
     1081 cgacgcccac gtcccgcatc agcaccggca ccgccctcgc ctcggcgttc cggagcgggc
     1141 cgcccgtcga gatccgcctg cacgaggtcg accgccggtg ggcgtcggcc accgagcagg
     1201 tcgaggcctc cagccgcagc gggttcgtcg ccacgcaggt cttcgagcaa gccgcccggg
     1261 cctcacgggc cgtcttcaag gcggccgacg agatcaccgt ccacgtcccc gagcacgagc
     1321 gcatgatggt cgaccagttc ggcgtggcgc cgggccgggt gcgcctcgtc gaccacggcg
     1381 aggacttcac ccgccgggtt cccgacgacc aggccgtcgc ccgggcctcg ctgggcctgc
     1441 ccgccgacgg ccacgtcttc gtgtgcatcg ggttcatcca gccccacaag gggttcgacc
     1501 gggccgccac ggccttccgc ggcctcggtg acaagggcgc ctcgctgcac gtcgtcgggt
     1561 cggtccgcgt cgacgacccg gccgcgctcg atcaccagcg cgagctcgag gccctcgagt
     1621 cccagatccc gggggtccac ctccacctcg ggttcgtgag cgacgaggcc ttcgaccggt
     1681 ggatcgtcgc cgccgacacc gtggtgctcc cctaccggca catctggtcg tcgtcggtcg
     1741 ccgaacgagc ccgcctctac gatcgcccgg tcatcgccac ccgcgtcggc ggcctggccg
     1801 accagatcgg cgagcacccc gccaacaccg tcgtcgacga caacgccggc ctcgcccggg
     1861 cgatggcggc ggccgtcggc gccgtcggcg ccgacggcga cgccgctcga tccgccgccc
     1921 cgacctggac cttcaccggt ccgatcgatc gtgacaccgt catggccgag atcacccgtc
     1981 gggcggcggc ccagcgcggg gttcggctca agccggtcac cgcccagcca ggggccgccg
     2041 ccgctcggtc gtcggctccc gcgcacgtcg ccaccccgct gcgccgcctc ggcctcaacc
     2101 cgccaccccc gccgacatcg gcccgtcccg gcgtgagcac ggtcaagcgg ctcatccgcc
     2161 gggtcatcgc gtgggaggtc gaccccctcc gcgagcagct cgtcgacctc cagcgtgcgg
     2221 cggtgaaagc cgccgagacg gccgacgccc ggctcgacga ggtcgccgac gaccagggcg
     2281 gctcgccgag cctctgaatc ggaggcgtcg atcgaggtct ccgacaccag cgggagcctc
     2341 tcgacggtgc tgcacggtcg tgccgctgcg gtggatcccg cgcgcgccct cgtgggggtg
     2401 ggtcgcctcg cccccacgag gcgcggcgga cccaccggtc ttcgaggggg tcagcggggg
     2461 ttcgtggcct cggcgatgat ccaggggaag gccttggtga ccttcacgtc cccgaagtgc
     2521 ccgtcgagcg cggtgcggag cttgcgaggg ctgaagaagt tcacgtgctc ggggtcgttg
     2581 ccccagcgct cgacgttctt gccccggccc aggttgccga ggcggaacca cggctcccac
     2641 ggcaccgaca ccacgagccg gccccggcag atgcgggcca tctccacgac cgcctgctcg
     2701 tagccgggga ggtgctcgag cacctcgatc gaagtcacca ggtccgcgga gcgatcgggg
     2761 aacgggagga cgccggcatc gccgcgcacc accgccacct cgggcagctt ctccgacgcc
     2821 acgagcgcct tgtcgtggcg gtactcgagg ccgacggccg gggtgtcggc cgggaacatg
     2881 cgctcgaggg cgaagccctc gccgatgccg atgtcgacca ccacgccgga ggtgtcgccg
     2941 agcacggtgt gcatcgtcgc catccagcgg gcgaggagct tctgcaccac cgggttcgac
     3001 gtgccgtact tggcggcgtc ctggcccgca gcagcgtcgt cgagcccggg agccaggccg
     3061 atggacttgg ggcgttcgtt cgccggcgcg gtcaccggag gtcgccttca gccgacgagt
     3121 cgagccggtc ccgccgccgg aggttcacca tcagctcgcc gagcaggccg agcgagatca
     3181 tctgcaccgc gaccacgatg agcagcacac cgagctggag caccggccgc tggccgatgt
     3241 tgtggccgag cagcttcgag gcgaacatcc acagcaagat ggcggcgccg ccgaagccga
     3301 tgccgaagcc gatgccgccg aagaggtgga acggccgcgc cgtgtacgtg gtgaggaact
     3361 tcaccgtgac caggtccagg aagccgcgcc agaaccgggc cggaccgaac ttcgaggtgc
     3421 cgtgcagccg ctcgtggtgc tcgacggtca cctcggtgac cttgaagccg ttccacacgg
     3481 caagcaccgg gatgtagcgg tggagctcgc cgtacatctc caggctgtcg gccagctccc
     3541 gggtcatgag cttgtagccc gagttgaagt ccttcatctt cacgccggtc accatcgagg
     3601 tggcgccgtt gtagaccttc gacgtgttgc gcttgatgaa gcggtcgttg cggacctcgc
     3661 ggcggccggt gacgaggtcg tagccctcgt cgagcttggc gaacatcttc ggcagctcgt
     3721 ccgggtcgtc ttggccgtcg gcgtccatca gcacgacgta ctcgccctgc acgtggttca
     3781 gcccgacctc gagcgcggcc gacttgccgg agttgcgacg cagccggatg tagcggacgt
     3841 tcgcgctgcg caggccggac atcaccgaac gggtgccgtc ggtggacccg tcgtcgacga
     3901 tgacgatctc ccagctgcgg ccgagcgcgt cgagcgccgc ggccgtcgcg gggatcacct
     3961 cgaccaggtt ctcggcctcg ttgtaggccg gcatgagcac agaaacgtca cagcccacgg
     4021 ccggcccctc gttcagacat cgggacaagg gtagttggtg gccctgccta gactcccagt
     4081 ccatgtcgcc ggggaaggtc ctgatcacag gtgcgcaggg cttcgtcgga cgccacctga
     4141 ccacccatct cgaagcctgc ggcgacgagg tcaccggcgt cgaccggctc ccggatccgt
     4201 ccggcaccaa cgacggcgtc gacatcaccg acgccggcgc catccgcgag ctcatcgcca
     4261 gcatcgggcc ccgcacgatc taccacctgg ccggctgggc cgacgtgggc ggttcgtgga
     4321 aggcgccggt cgaggcgttc cgggccaacg ccgagggcac gctcaacgtg ctcagcg
//
LOCUS       DATMFS010000210         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_262572, whole genome shotgun sequence.
ACCESSION   DATMFS010000210 DATMFS010000000
VERSION     DATMFS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262572"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..652
                     /locus_tag="VNQ33_03910"
     CDS             227..652
                     /locus_tag="VNQ33_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWJ97279.1"
                     /translation="MASRTKHLEHLAEIPLFSALSKRDLGRIAKASNEVTVPAGHVLV
                     DQGDAGREAFVIVEGTATVKRNGRKVGTLGPGDSIGELALLDHGPRTATVTADTDLAT
                     LVLSAREFASVVEEVPGLAQKLLAQLAARVRELDRQIYG"
     gene            801..1601
                     /locus_tag="VNQ33_03915"
     CDS             801..1601
                     /locus_tag="VNQ33_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97280.1"
                     /translation="MRTTTARGARIGILVSAVALVATACQRTEVVLYDASKKPNLAFH
                     HITRDGSDRYDLKVGTGALRVNAPRTNKGTPQGSTTRIAIWPPATKMTTDQQSCATWK
                     DDQGAWAQEGLALRIRIDGSRFRAIVVAKNIVYGANWQLNVYTWDSGRTPYFTTHGAV
                     SLRTPFASGDVPQPLPWKVCARAEGRIVTIKGWRLDESEPGWSNPQHTGTVTLPSEWV
                     FAGKAGWYAGHIDPGGTIGMGGLRTSTWVVTDKGGSPGTKGAPTAPGA"
     gene            1650..>2758
                     /locus_tag="VNQ33_03920"
     CDS             1650..>2758
                     /locus_tag="VNQ33_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HWJ97281.1"
                     /translation="MATEETTADKRIKPHHLALGLGVGVAAFTAVSGITPLITKWHDE
                     SPIGREVFFNIPGWMKLAFYIVVPMFIVYGAYMFSLRMRNWERGQPDKRATTTKNVKT
                     RAENYRSGVYMQTLLRDPAAGVMHSFIYFSFLVLLAVTTVLEINHQVPVSWKFLHGTT
                     YQAYSLVGDTAGIFLFVGVSWAIIRRYVARPYRIRIKTKSDHALGLGTLFALALTGFL
                     AEGWRIALEGTPAFEKWSIVGYPIATAVDGMGSLSTMHRAWWLVHVASFLTFLFILPV
                     TMMRHMFTSPLNMFLRDKDRPKGAMRPMPNLMETELENFGASVIEDFTWKQLLDTDSC
                     TMCGRCTAQCPAHLTGKPLDPREIVLKVGEVMARTG"
BASE COUNT          448 a         1033 c          876 g          401 t
ORIGIN      
        1 tcgacatccc caccggggtg ccgctcgtct acgagctcga cgacgacgcc cgcccgacgg
       61 agtccacatc cgccctcgaa cgagccctgg gcgaccccga agccatcaag gccgccgccg
      121 atgccgtcgc ccgccaagcc acccgaggcg cctgacccgc tccctcgccg ccgccccgac
      181 cgcgatctgc ggcacgggcc gtggcgcgtc cgtaagctgc cccgccatgg ctagccgcac
      241 gaagcacctg gaacacctcg ctgagatccc gttgttctcg gcgctgtcga agcgagacct
      301 cggacgcatc gccaaggcgt cgaacgaggt gacggtcccg gcgggccacg tgctcgtcga
      361 tcagggcgac gccggtcggg aggcgttcgt gatcgtcgag gggacggcca ccgtcaagcg
      421 caacgggcgg aaggtcggca cgctcggccc cggcgactcc atcggcgaac tggcgctgct
      481 cgaccacggg ccccgaacgg ccaccgtcac cgccgacacc gacctggcca ccctcgtgtt
      541 gagcgcccgc gagttcgcca gcgtggtcga ggaggttccg ggcctggcgc agaagctgct
      601 cgcgcagctg gcggcgcgcg tgcgcgagct cgaccgccag atctacggct gagaccggcc
      661 tcggcggccc cgggcggcag cgagcctccg cgggtgaacg acgtgtcacc cgtcacagaa
      721 tcacgacatg agatgatgtc gtctgcctag cgtgtcggga tcagatcggc cgtgggaggt
      781 ccgatcggca gggggccacc atgcggacga ccacagctcg cggtgcacgc atcggcatcc
      841 tggtgagcgc cgtcgcgctc gtcgccaccg cctgccagcg caccgaggtc gtgctctacg
      901 acgcctccaa gaagccgaac ctggccttcc accacatcac ccgcgacggc agcgaccgct
      961 acgacctcaa ggtcggcacc ggcgccctgc gggtgaacgc cccacgcacc aacaagggca
     1021 cgccgcaggg ctcgaccacg cgcatcgcca tctggccgcc ggccaccaag atgaccaccg
     1081 atcagcagtc gtgcgccacc tggaaggacg accagggggc gtgggcccaa gagggcctcg
     1141 ccctgcggat ccgcatcgac ggctcgcgct tccgggccat cgtcgtcgcc aagaacatcg
     1201 tctacggcgc caactggcag ctgaacgtct acacctggga ctccggtcgg acgccctact
     1261 tcacgaccca cggcgccgtc tcgcttcgca ccccgttcgc gtcgggagac gtgccccagc
     1321 cgctgccctg gaaggtgtgc gcccgagccg agggccggat cgtgacgatc aagggctggc
     1381 gtctcgacga gtccgagccg gggtggtcca acccgcagca caccggcacg gtgaccctcc
     1441 cgtcggagtg ggtcttcgcc ggcaaggccg gctggtacgc gggccacatc gaccccggcg
     1501 ggacgatcgg gatggggggc ctgcgaacgt ccacgtgggt cgtcaccgac aagggcggat
     1561 caccgggcac gaagggcgct cccacggccc ccggggccta gctgccaaat cggtgccgct
     1621 caggcggcgc gagtaccttt gcccttgtta tggctaccga agagaccaca gctgacaagc
     1681 ggatcaagcc gcaccacctg gccctcggtc tgggcgtggg ggtcgccgcg ttcaccgcgg
     1741 tgtcgggcat cacgcccctc atcaccaagt ggcacgacga gtcgcccatc ggccgtgagg
     1801 tcttcttcaa catccccggg tggatgaagc tcgccttcta catcgtcgtc ccgatgttca
     1861 tcgtctacgg cgcctacatg ttctcgctgc ggatgaggaa ctgggagcgg ggccagcccg
     1921 acaagcgggc caccacgacc aagaacgtca agacccgcgc cgagaactac cgctccggcg
     1981 tctacatgca gaccctgctg cgcgaccccg ccgccggcgt catgcacagc ttcatctact
     2041 tctcgttcct ggtgctgctg gccgtcacca cggtcctcga gatcaaccac caggtgcccg
     2101 tgagctggaa gttcctgcac ggcaccacct accaggcgta ctcgctggtc ggcgacaccg
     2161 ccggcatctt cctcttcgtg ggcgtcagct gggcgatcat ccgccgctac gtcgcccgcc
     2221 cgtaccggat ccgcatcaag accaagtccg accacgcgct cggcctcggc acgctcttcg
     2281 ccctcgccct caccggcttc ctcgccgagg gctggcgcat cgccctcgag ggcacgcccg
     2341 ccttcgagaa gtggtcgatc gtcgggtacc ccatcgccac ggccgtcgac ggcatgggga
     2401 gcctctcgac catgcaccgc gcctggtggc tcgtccacgt ggcctcgttc ctcacgttcc
     2461 tcttcatcct ccccgtgacg atgatgcgcc acatgttcac gtcgccgctc aacatgttcc
     2521 tgcgggacaa ggaccgcccg aagggcgcca tgcggccgat gccgaacctc atggagaccg
     2581 agctggagaa cttcggtgcg tcggtcatcg aggacttcac ctggaagcag ctgctcgaca
     2641 ccgactcgtg caccatgtgc gggcggtgca cggcccagtg cccggcccac ctgacgggca
     2701 agccgctcga cccccgcgag atcgtgctca aggtgggcga ggtcatggcc cgcaccgg
//
LOCUS       DATMFS010000211         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_8575, whole genome shotgun sequence.
ACCESSION   DATMFS010000211 DATMFS010000000
VERSION     DATMFS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8575"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VNQ33_03925"
     CDS             complement(<1..2045)
                     /locus_tag="VNQ33_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97282.1"
                     /translation="MRNNRWVWIGVYVVATALLAGGVVFLIQDRDQSSEAAAPVLPEL
                     ATMPFASGLTPVGPTTGRVGDSLLFDAEGGTEDVRSVELWDGDRRIETQAVDGTPGAV
                     VTLPALSVGQHAVYAKVTNADGEASLTAPVPVAIVANGTEPVPPVTVVPDEDETLGQL
                     AERLDIGIDRISVLAAGSDLLPPEQVKDLPLAAGDVIPVDAIVGAQPDGDAGYVEAVS
                     ADPVVPEGGGASTGDLALSAEAAGCQITLTVSGASGKTQVYEAAGSAVGWTRVGERSG
                     DGTVSIPVATPGVHLFYALAGNEQTTPLQVTMPPGCTKASGWTGNAFITDGILTVTTG
                     VGGAGQWLYLQADGGTAVRVPATGYLPPVGLYTDIGALLPPMAGTTLDLELWQGSEFL
                     DTKVASGHLNAPEGRSIGDVTGEGRQLDLKLTDPAGTAAILGSQDRKLTFTWTAGARI
                     EAVLYQVLVDDQPITQTRVPPEQLLASGVSIRSSAGAAGSGTFTINTADIPGRQATGR
                     EATAGATASYEAKTAQLTDKNIVSSNKPVSVTPIGPGTVKRAAEAPIVIDLPTYGDNV
                     YVRAIGIGSEGSGVTSASNTIRVQLPVPQGIGGKAVDFVTGDDFGDTTFAPGHRPVRS
                     FQNCMWADVGWFGPIDYGKYLDRSPHGQSARIEAMSAAYPADGTYCPQDFPPPPPPPS
                     "
     gene            2160..>2417
                     /locus_tag="VNQ33_03930"
     CDS             2160..>2417
                     /locus_tag="VNQ33_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97283.1"
                     /translation="MLLAKLAIPLAMATVGAGAAVAAVVTDEPASTQVSTAAQVWIDA
                     PTGEEPVAPGSVHVGAHATATGSMGSLVLYVDGKQVATDSEL"
BASE COUNT          315 a          850 c          886 g          366 t
ORIGIN      
        1 gagggcggcg gaggcggtgg tgggaagtcc tgcgggcagt aggtcccgtc cgcggggtag
       61 gccgccgaca tggcctcgat ccgcgccgac tgtccatggg gagatcggtc gaggtacttg
      121 ccgtagtcga tcggcccgaa ccagccgacg tcggcccaca tgcagttctg gaacgaccgc
      181 accggacggt ggcccggtgc aaacgtggtg tcgccgaagt cgtcgcccgt gacgaagtcg
      241 acggccttgc cgccgatgcc ctgggggacc gggagctgga cccggatggt gttcgatgcc
      301 gatgtcacgc ccgagccctc cgagccgatg ccgatggcgc gcacgtagac gttgtcgccg
      361 taggtcggca ggtcgatgac gatgggggct tcggccgccc gcttcacggt gccgggcccg
      421 atcggcgtga ccgacacggg cttgttgctc gacacgatgt tcttgtcggt cagctgggcg
      481 gtcttggcct cgtacgacgc ggtcgccccg gccgtcgcct ccctcccggt ggcctgcctc
      541 ccggggatgt cggcagtgtt gatcgtgaag gtgcccgagc ccgcggcacc agcggagctc
      601 cggatgctga cgccgctggc gagaagctgc tcgggtggca ccctcgtctg ggtgatcggc
      661 tgatcgtcga cgaggacctg gtacagcacg gcctcgatcc gggcgccggc ggtccaggtg
      721 aaggtgagct tgcgatcctg tgagccgagg atcgcggcgg tgccggccgg gtccgtgagc
      781 ttcaggtcca gctggcggcc ctcgccggtc acgtcgccga tggagcggcc ctccggggcg
      841 ttcaggtggc cgctcgccac cttcgtgtcg aggaactcgc tgccctgcca gagctcgagg
      901 tccagggtgg tgccggccat cggtggcagc agcgcgccga tgtccgtgta caggccgacc
      961 ggtgggaggt accccgtcgc gggcactcgc acggcggtgc cgccgtcggc ttggaggtag
     1021 agccactgcc cggcgccgcc gacccccgtc gtcacggtga ggatcccgtc ggtgatgaac
     1081 gcgttccccg tccagcccga cgccttggtg cagcccgggg gcatcgtcac ctgcagcggc
     1141 gtcgtctgct cgttgcccgc cagcgcgtag aagaggtgca cgcccggggt ggcgaccggg
     1201 atggacaccg tgccgtcgcc ggaccgctcg ccgacgcgcg tccaccccac ggcactgccc
     1261 gctgcctcgt agacctgggt cttgccgctc gcgccggaga cggtgagcgt gatctggcag
     1321 ccggcggcct cggccgacag cgccaggtcg ccggtcgacg cacctccgcc ctccggaacg
     1381 actggatcgg ccgacaccgc ctccacgtag ccggcgtcgc cgtcaggctg ggcgccgacg
     1441 atggcatcga cggggatcac gtcgccagcc gcgaggggca ggtccttcac ctgctcgggc
     1501 ggcaggaggt ccgatccggc ggcgaggacc gagatgcggt cgatgccgat gtcgaggcgc
     1561 tcggcgagct gccccaaggt ctcgtcctcg tcgggcacca ccgtgaccgg cggcacgggt
     1621 tcggtgccgt tggcgacgat ggcgacgggg acgggcgcgg tcagcgacgc ctccccgtcg
     1681 gcgttggtca ccttggcgta gacggcgtgc tggccgaccg acagcgccgg cagcgtgacc
     1741 accgcgccgg gtgtgccatc caccgcctgg gtctcgatgc gccggtcgcc atcccacagc
     1801 tcgacgctgc ggacgtcctc cgtgccgccc tcggcgtcga acagcaggct gtcgccgaca
     1861 cgaccggtgg tgggaccgac cggcgtcagc cccgatgcga acggcatcgt ggccagctcg
     1921 ggcagcacgg gtgccgccgc ctcgctgctc tggtcgcggt cttggatcaa gaagacgacg
     1981 ccgccggcga ggagcgccgt ggcgaccacg tacaccccga tccacaccca tcggttgttg
     2041 cgcatccgcg gaacctaagc ccgcgctccc tcctggctca tccgatcggc gacatctggg
     2101 accagatcgg gaccgggtgc ccctcctctt gcgggtgcgg gggttcgtag ggttgcggcg
     2161 tgctcctcgc caagctcgcc atcccgctcg ccatggccac cgtcggagcg ggagcggccg
     2221 tcgccgcggt cgtgaccgat gagccggcat cgacgcaggt gtcgacggcg gcacaggtgt
     2281 ggatcgacgc accgacgggg gaggagccgg tcgcacccgg gtccgtccac gtgggggccc
     2341 acgccaccgc caccggctcg atgggctcgc tcgtcctcta cgtcgacggc aagcaggtcg
     2401 ccaccgactc cgagctc
//
LOCUS       DATMFS010000212         3730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_77599, whole genome shotgun sequence.
ACCESSION   DATMFS010000212 DATMFS010000000
VERSION     DATMFS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77599"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VNQ33_03935"
     CDS             <1..1920
                     /locus_tag="VNQ33_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97284.1"
                     /translation="KPGATVVLEGPDGSTAEVTEAGTSKVGSTQLETRTIAGRGYAID
                     LVRPADDASWSGEWKASITDPDQEGDDATLQVFVFSDIGVAFAGKPQLERGAATELTV
                     KLALPKGVKAADVIEASEAKVRLRNPVTGQVDEIPLEGKPGGPFTGSFTTPGTLTSNV
                     VEATAEVHITTTSDAELVSQSAPVDVLVLRPKGSIQFIPGSLQMPSLTGSGTTKGDMT
                     LVGGSADGCVWFGRAAVPEPPEGAAPIAVTLADGAPLPGEDGCIAVPKGEIVTISVEA
                     APDGRASGTVAGTLTVHEKVEGRQKASTTEVPFRFDMARGVDQARRLLLSIVMLVGGL
                     GLPMLALILLNAVTARFQDLDAVQGTALPVQVQQSQISRIDGSYPRPLSLRSDDFGSL
                     ATAGSNRRFTFGGVEFRARASRNPFGATIAMAAPEGGAAKLKGKVGSRVELDPALAGS
                     WIFLLDSDKTRLAAHGEVVGLLIAFVAEGSVAPQTDRMLSDITLRLPGIAAGLAGLVR
                     RVKVKAKKAKGGDRTGSATDGTGLEGEGSADPAMEPGSALPSDDAGPATTDAVDSDSD
                     SDSDADPVPEPEAEPEGAPESEPAGEADADAPVPPVGFGGVAPGGVPPRPSDDPLPPD
                     DDDPGGPPVGFTGVR"
     gene            complement(1931..2818)
                     /locus_tag="VNQ33_03940"
     CDS             complement(1931..2818)
                     /locus_tag="VNQ33_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWJ97285.1"
                     /translation="MAVVFGLLVALFYGSGDFFGGLAAKRTAAITVVIGSFALSTALV
                     FAATAGWFVVGTPPTPATSDLWLGVAVGLTGPAAVGLLYRGLAMGRMSVVAPITAVVA
                     AIVPLVWALADGERPSWIALCGVAVALVSIVMVAAAPEHDEHPDDAAVAPLSKVVPPA
                     VASGLGFGALYVELGETSTHAGLWPLFVARPVAVAVTAVAALALARRARRPASAIVPA
                     RSAWPFVAGAGVLDVTANAIYLAATNAGLLSIVAVLSSLYPAATVVLARIVLGERLHR
                     VQVTGLVLAAAGIAAMATG"
     gene            2859..>3730
                     /locus_tag="VNQ33_03945"
     CDS             2859..>3730
                     /locus_tag="VNQ33_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWJ97286.1"
                     /translation="MADEILEVDLLAFERGSAEQQRAVVDGLMRSLSTGFVYVTHDVS
                     GDLIDEAYALLEQFFTLPADTKARFVAPGTHGQTGYTGLLVETAASSDVPDWKEMLNW
                     ARDVPPGHPLRTRFPHRYHDQVLPEAAVPGIAHVLNTFHDAIADLQRRILRIIAVGIG
                     GHVDLFEKMLVDGSTLSRAIHYPAMEAAPDAPHVWAGEHADINLITALPRATARGLEV
                     KLKDEDRWIDAVAPHDGAIINTGLMLEIVTNGVITPGIHRVVAHADQPGDRYSVVQFC
                     HPTPWTILSPVATCV"
BASE COUNT          573 a         1409 c         1298 g          450 t
ORIGIN      
        1 aagccggggg cgaccgtcgt cctcgaggga cccgacggga gcaccgccga ggtgaccgag
       61 gcggggacct ccaaggtggg cagcacccag ctcgagaccc gcaccatcgc cggccgcggc
      121 tacgcgatcg acctcgtgcg cccggccgac gacgcctcct ggagcggtga gtggaaggcg
      181 tcgatcaccg accccgacca agaaggcgac gacgccaccc tccaggtgtt cgtcttcagc
      241 gacatcgggg tggcgttcgc cggcaagccg caactcgagc gtggcgccgc caccgagctc
      301 acggtgaagc tcgccctgcc caagggcgtg aaggccgccg acgtgatcga ggcgtcggag
      361 gccaaggtcc ggctccgcaa cccggtcacc ggccaggtcg acgagatccc gctcgagggg
      421 aagcccggtg ggccgttcac cggcagcttc acgactcctg gcaccctcac ctcgaacgtg
      481 gtcgaagcca cggccgaggt gcacatcacc accacgagcg atgccgagct ggtgtcgcag
      541 tccgccccgg tggacgtcct cgtcctccgc cccaagggct ccatccagtt catcccggga
      601 tccctccaga tgccgtcgct gaccggaagc ggcacgacca agggcgacat gaccctggtc
      661 gggggctcgg cagacgggtg cgtgtggttc ggcagggccg ccgtgcccga accgcccgaa
      721 ggggccgcgc ccatcgccgt caccctggcc gatggcgcgc cgctgcccgg cgaggacggc
      781 tgcatcgccg tcccgaaggg cgagatcgtc acgatctcgg tcgaggcggc gcccgacggc
      841 cgggcatcgg gcacggtggc ggggacgctc acggtgcacg agaaggtgga gggccgccag
      901 aaggcgtcca ccaccgaggt cccgttccgg ttcgacatgg cccgcggcgt cgatcaggcc
      961 cgacgcctgc tgctcagcat cgtgatgctg gtcggcggcc tcgggctgcc gatgctggcg
     1021 ctcatcctct tgaacgcggt cacggcccgc ttccaggacc tcgacgccgt gcagggcacc
     1081 gccctgccgg tccaggtgca gcagagccag atctcccgca tcgacggcag ctacccgcgg
     1141 ccgctctcgt tgcggtccga cgacttcggc agcctcgcca cggccggcag caaccggcgc
     1201 ttcacgttcg gcggcgtcga gttccgcgcc cgcgcctcgc gcaacccgtt cggggccacc
     1261 atcgccatgg ccgctcccga aggcggtgct gccaagctca agggcaaggt cggcagccgc
     1321 gtcgagctcg acccagccct cgccggttcg tggatcttcc tcctggattc cgacaagacc
     1381 cgcctggccg cgcacggcga ggtcgtcggg ctgctcatcg ccttcgtggc cgagggcagc
     1441 gtcgcgccgc agaccgatcg catgctctcg gacatcaccc tgcgcctccc gggcatcgcg
     1501 gcgggcctgg cgggcctggt gcggcgggtc aaggtgaagg ccaagaaggc caagggcggc
     1561 gaccgaaccg gcagcgccac cgatggcacg ggcctcgaag gcgaagggtc ggccgatccg
     1621 gcgatggagc ccggatcggc ccttccgtcc gacgacgccg gtccggcgac gaccgatgcc
     1681 gtggactcgg actcggactc ggactcggac gccgacccgg tgcccgagcc cgaagccgag
     1741 cccgaaggcg cgccggagtc cgagcccgca ggcgaggcgg acgcggacgc gccggtgccc
     1801 ccggtcggct tcggtggcgt cgcccctggc ggcgtccccc cgcgcccgtc cgacgacccg
     1861 ctcccgcccg acgacgacga tccgggcggc ccgccggtgg gcttcaccgg ggtccggtag
     1921 cgccgccccg tcagccggtg gccatggcgg cgatgccggc cgcggccagc accagcccgg
     1981 tgacctgcac gcggtgcagg cgttcaccca agacgatgcg tgccagcacc acagtggccg
     2041 ccgggtagag cgacgacaag acggccacga tcgacagcag cccggcgttg gtggcggcga
     2101 ggtagatggc gttggccgtc acgtcgagca cgccggcccc ggccacgaac ggccacgccg
     2161 aacgggcggg gacgatcgcc gaggcgggcc gtcgggctcg cctcgccagc gcgagcgcgg
     2221 cgacggccgt caccgccacg gccaccggcc gggcgacgaa caggggccac agcccggcgt
     2281 gggtgctggt ctcgccgagc tccacgtaga gcgcgccgaa cccgagtccc gatgcgaccg
     2341 ccggcggcac caccttggac agcggggcga ccgctgcgtc gtcggggtgc tcgtcgtgtt
     2401 cgggggccgc tgccaccatc acgatcgaga ccagggcgac ggcgacaccg cagagcgcga
     2461 tccacgacgg gcgctcgccg tcggccagcg cccacaccag cggcacgatc gcggcgacga
     2521 ccgccgtgat cggcgcgacg acgctcatgc ggcccatggc caggccgcgg tacagcaagc
     2581 cgacggcggc aggaccggtg agcccgaccg ccacacccag ccagaggtcg ctggtcgccg
     2641 gggtcggcgg ggtcccgacg acgaaccagc cggccgtcgc cgcgaacacc agcgccgtgc
     2701 tcaaggcgaa cgagccgatg accaccgtga tcgccgcggt ccgcttggcg gcgagcccgc
     2761 cgaagaagtc gccggacccg tagaagaggg ccacgagcaa cccgaagacg acggccatag
     2821 ggcggcgacg gtactgcctc gtgggtagcg tcagaaccat ggctgacgag atcctcgagg
     2881 tcgacctcct cgcgttcgag cgggggagcg ccgagcagca gcgggcggtg gtcgatggcc
     2941 tgatgcggtc cctgtccacc gggttcgtgt acgtgaccca cgacgtgagc ggcgacctga
     3001 tcgacgaggc gtacgccctg ctggagcagt tcttcaccct cccggcggac accaaggccc
     3061 ggttcgtggc acccgggacg catggccaga ccgggtacac cggcctcttg gtggagacgg
     3121 cggcctcgtc cgacgtcccg gactggaagg agatgctcaa ctgggcgcgc gacgtgccgc
     3181 ccggccaccc gctccgcacc cgcttcccgc accgctacca cgaccaggtc ctgcccgagg
     3241 ccgccgtgcc gggcatcgcc cacgtcctca acacgttcca cgacgccatc gccgatctgc
     3301 agcgccgcat cctgcgcatc atcgccgtcg gcatcggcgg ccacgtggat ctcttcgaga
     3361 agatgctcgt cgacgggtcg accctcagcc gggccatcca ctacccggcg atggaggccg
     3421 cccccgacgc gccccacgtg tgggcgggcg agcacgccga catcaacctc atcaccgcgc
     3481 tgccccgggc caccgcccgt gggctcgagg tgaagctcaa ggacgaggac cggtggatcg
     3541 acgccgttgc tccccacgac ggcgccatca tcaacaccgg cctgatgctc gagatcgtca
     3601 ccaacggcgt catcacccct ggcatccacc gggtcgtcgc ccacgccgac cagcccggcg
     3661 accgctactc ggtcgtgcag ttctgccatc cgaccccgtg gacgatcctc agcccggtcg
     3721 ccacctgcgt
//
LOCUS       DATMFS010000213         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_124826, whole genome shotgun sequence.
ACCESSION   DATMFS010000213 DATMFS010000000
VERSION     DATMFS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124826"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VNQ33_03950"
     CDS             <1..759
                     /locus_tag="VNQ33_03950"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ97287.1"
                     /translation="HLEILRVEAGRLASIDPSKLGAAVPSIEGWDVERVVRHVGKVHE
                     WVLGMLRLEPGQGLGGAPVPHGLPKGPDCLPAYRAVAGELIAHLEADDPSRPCLNFAG
                     TGDVAWWMRRQAQEVSVHRVDAHDALHAAGGPAPDLIEVDGAADGIDEWARFFLAIRW
                     AQRFGELPEDLAGRTVHIHGTDDPAPADGAEWLLSFTADGVQVEATHAKGDVALRGSA
                     NDLLLTLWRRRPLRALDVLGDAAVAERLLDVARF"
     gene            802..2502
                     /locus_tag="VNQ33_03955"
     CDS             802..2502
                     /locus_tag="VNQ33_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWJ97288.1"
                     /translation="MRRIAPLLLASALVLAACSSGGSDAGQDSTTTKPKGSASTTTGA
                     GGGETAGGGHACDQGAAFDEVPVEPVAGVDSDRTMTSPVDGTGIRLHWFPVAGASADD
                     PAPTILMGPGWSLPGDTSIDGDALFSALTIGSMRKDGYNVLTWDPRGFGKSGGTATVN
                     DPGKEGQDVRAMLDFVASQPGAQTDGEGDPRAGMVGFSYGGGIQLTTASIDCRVDAIV
                     PGIAWNSLQTSLYKADTVKTGWAKVLTSAVDPDRLDPHINSASASGLRDGTLSDEDRD
                     WFVSRGPGDDAIAKIDIPTLFVQGTVDTLFSLDEATTNYGILRKDDVPTAMLWFCGGH
                     GTCLTDPGDRGRVAAASFAWLDHYLKGEDTDLPAGVDLVDQDGRRWTAEDWPVAEGEP
                     LTGTGSGTLALKAEGGSGGDPTALDLDPDDPLGGVVASITPTQADNAVDVAIDAGDAD
                     VLALGAPKLTITYSGDWTGDVQGAEGRVFAQLVDDETGLVLGNQITPIQVTVDGTTHT
                     AESDLEIIAHHVTPGHGITLQIVAATSAYGIPVKQGTIDFDSIEISVPTAAPKAVAQG
                     "
     gene            2520..>2799
                     /locus_tag="VNQ33_03960"
     CDS             2520..>2799
                     /locus_tag="VNQ33_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWJ97289.1"
                     /translation="MDLPVMPPLLPMLAKASPELPDAEPGAYWFEPKWDGFRCIVFRD
                     GDEIELGSRNDRPLTRYFPELLEPLKASLPDKCVVDGEVVVAGPDGLDF"
BASE COUNT          410 a         1040 c          981 g          368 t
ORIGIN      
        1 catctggaga tcttgcgggt ggaggcgggg cggctcgcct ccatcgatcc gtcgaagctg
       61 ggcgcggccg tgccgtccat cgagggatgg gacgtcgagc gggtcgtgcg gcacgtcggc
      121 aaggtccacg agtgggtgct cgggatgctc cggctcgagc ccggccaggg gctcggcggt
      181 gcgccggtac cgcacggtct gcccaagggg cccgactgcc tgcccgccta ccgcgccgtg
      241 gccggcgagc tgatcgccca cctcgaggcc gacgacccga gccggccttg cctcaacttc
      301 gccggcacgg gcgacgtggc ctggtggatg cggcggcagg cgcaggaggt gtcggtgcac
      361 cgggtcgatg cgcacgatgc cctccacgcg gccggcggtc cggcgcccga cctgatcgag
      421 gtcgacggcg ccgctgacgg catcgacgag tgggcccggt tcttcctggc gatccgctgg
      481 gcgcagcgct tcggcgagct gcccgaggac ctggccggcc gcaccgtgca catccacgga
      541 accgatgatc cggcaccggc cgacggagcc gagtggctgc tgtcgttcac cgccgacggc
      601 gtgcaggtgg aggccaccca cgccaagggc gacgtcgccc tgcgcggctc ggccaacgac
      661 ctgctcctca ccctgtggcg gcgccgaccg ctccgcgccc tcgacgtcct cggcgatgcg
      721 gccgtggccg agcggctgct cgacgtggcg cggttctgac gctccgtcag gacgacgcca
      781 ccaggagtag cgtcgcggct cgtgcgccgc atcgctcctc tcctgctcgc cagtgccctc
      841 gtcctcgccg cctgcagctc cggcggcagc gatgcgggcc aggactcgac caccacgaag
      901 ccgaagggca gcgcgtccac cacgaccggc gcgggtggtg gcgaaaccgc cggcgggggc
      961 catgcgtgcg accagggcgc ggcgttcgac gaggtgccgg tcgagccggt ggccggggtc
     1021 gacagcgacc ggaccatgac ctcgcccgtc gacggcaccg gcatccggct gcactggttc
     1081 ccggtcgcag gggcgtcggc cgacgatccc gcgcccacca tcctcatggg tccgggctgg
     1141 tcgctccccg gcgacacgtc catcgacggc gacgcgctct tcagcgcgct gaccatcggg
     1201 agcatgagga aggacgggta caacgtcctc acctgggacc cgcggggctt cgggaagtcg
     1261 ggcggcaccg ccacggtgaa cgacccgggc aaggaaggcc aagacgtgcg ggcgatgctc
     1321 gacttcgtgg cctcccagcc cggagcccag accgatggcg agggcgatcc ccgcgccggg
     1381 atggtcggct tctcctacgg cggcggcatc cagctgacca cggcgtcgat cgactgccgg
     1441 gtcgacgcca tcgtccccgg catcgcctgg aactcgctgc agacgagcct ctacaaggcc
     1501 gacaccgtga agaccggctg ggcgaaggtc ctgaccagcg cggtcgatcc cgaccggctc
     1561 gacccgcaca tcaactcggc ttcggcatcg ggcctgcgcg acggcacgtt gagcgacgag
     1621 gaccgcgact ggttcgtcag ccgcgggccg ggcgacgacg ccatcgccaa gatcgacatc
     1681 ccgacgctgt tcgtccaggg caccgtcgac accctcttca gcctcgacga ggccaccacc
     1741 aactacggga tcctgcggaa ggacgacgtc ccgacggcga tgctgtggtt ctgcggcggg
     1801 cacggcacct gcctgaccga ccccggtgac cgcggccgcg tggccgccgc cagcttcgcc
     1861 tggctcgacc actacctgaa gggcgaggac acggacctgc cggcgggtgt cgacctcgtc
     1921 gaccaggacg gcaggcgctg gacggccgag gactggcccg tcgccgaggg cgaaccgctc
     1981 acgggcacgg ggtcgggcac cctcgccctc aaggccgagg gcggcagcgg cggcgacccg
     2041 accgcactcg acctggaccc cgacgaccct ctcggcggtg tcgtcgcctc gatcaccccg
     2101 acccaggccg acaacgcggt cgacgtggcc atcgacgcgg gcgacgccga cgtgctggcc
     2161 ctcggcgccc ccaagctgac catcacgtac tccggcgact ggaccggcga cgtgcagggc
     2221 gccgagggcc gggtgttcgc ccagctggtc gacgatgaga ccgggctcgt gctcggcaac
     2281 cagatcaccc cgatccaggt cacggtggac ggcacgacgc acaccgccga gagcgatctc
     2341 gagatcatcg cccaccacgt gacccccggc cacgggatca cgctccagat cgtcgccgcc
     2401 accagcgcct acggcatccc ggtgaagcag ggcacgatcg acttcgactc gatcgagatc
     2461 tccgtcccga cggccgctcc gaaggccgtc gcacaaggct gacccctaac ctcgccggca
     2521 tggacctgcc ggtgatgcca cccctgctcc cgatgctcgc caaggcgtcg ccggagcttc
     2581 ccgacgccga gccgggggcg tactggttcg agccgaagtg ggacgggttc cgctgcatcg
     2641 tgttccgcga cggtgacgag atcgagctgg gttcccgcaa cgaccggccg ctcacccggt
     2701 acttccccga gctgctcgag ccgctgaagg cctcgctccc ggacaagtgc gtggtcgacg
     2761 gcgaggtcgt cgtcgccggc cccgatgggc tcgacttcg
//
LOCUS       DATMFS010000214         3647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_31895, whole genome shotgun sequence.
ACCESSION   DATMFS010000214 DATMFS010000000
VERSION     DATMFS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31895"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VNQ33_03965"
     CDS             complement(<1..1042)
                     /locus_tag="VNQ33_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97290.1"
                     /translation="MGFNIADLFERAVDAVPDRRALVCGDRTLTFAEFDAEANQLANH
                     LLAEGFGHGDHIGIYGQNSAEWMIAMVAAFKIRAVPININFRYVEDELAYLFDNADLV
                     ALVHSREYIDRIEAVTAKVPGLRHFVMIDDGSDLDPARIGAVSWADAIAGASPERPQL
                     PRTDDDHYVLYTGGTTGMPKGVVWRHEDVFYALGGGVDAYTNERVTHGMQLAEKAAAS
                     EAPMVTLNAPPLMHGAAQWGALRFFFEGNTVVFVPKFSPEEVWATIERDRVTTIVITG
                     DAMARPLVECLHEHPDRWDLSSLFVVSSSAAIFSPTLKDQLLDLLPNIIIVDAIGSSE
                     SGMNGMVIQTKGE"
     gene            complement(1174..2661)
                     /locus_tag="VNQ33_03970"
     CDS             complement(1174..2661)
                     /locus_tag="VNQ33_03970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWJ97291.1"
                     /translation="MTGLDAAFLYLENPTNHMHVAMTMVLDPSTVPGGYSFENLKEFI
                     RGRLHLVPPFTRRLCEVPLNLAHPLWVEDPNFDLDFHIRRIGCPAPGGRRELGEMAGQ
                     IASTPLDRSRPLWELWVIEGLKQDRIGVVTKVHHAAIDGSSGAELMVHLFDLEPEAAE
                     PPPPADHEPEHIPNDFELLGHAAASRVRKLIALPKLIGDTVVSVNRVAQGRRSSEEGK
                     TGAAPLTAPRTSWNGSLSPLRSVGFARVTLDDVKALKDAFGCTVNDVVLGLCAGTLRS
                     YMQANEDVPDTPLVAACPVSVRAAEDAAPGNKVSAMFATLDTQLDDPVERIRAIQACT
                     VGAKEEHKAVGADMLQNWAEYAAPNTFNLASRLYSSWGLADSHRPIHNVIISNVPGPG
                     FPLYYAGAEMVAAYPMGPVMEGVGLNITVMSYRGSVDFGFMVDRELVSDVWDMADRVK
                     PALEELQAAAKLLAEGAEPTVDPGSPVDADGIPPANPAPMRADLT"
     gene            2793..3494
                     /locus_tag="VNQ33_03975"
     CDS             2793..3494
                     /locus_tag="VNQ33_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97292.1"
                     /translation="MTDLAESPARAIADLPGGPYDIEFFFDPGCPFAWQTSVWVRRVV
                     ELRGIKVGWRFISLKFINEDKDEYMPGMRDAQHQGLRYHRICAAAREQFGNGAVAGLY
                     KAWGEAYWNTEAEGEFPERIKAGFANAVPADLVASVGLPESILDAADDESWDAQIRAE
                     SDEAFRRTGDDVGTPIITYLPSGNSLFGPVISSVPDDETALKFYDALRVFADFPQFSE
                     LKRTKRAPLDLPLFN"
BASE COUNT          547 a         1184 c         1325 g          591 t
ORIGIN      
        1 tctcgccctt ggtctggatg accatgccgt tcatgccgct ctcggaggat ccgatggcat
       61 ccacgatgat gatgttcggc agcaggtcga gcagctggtc cttgagcgtc ggcgagaaga
      121 tggcggcgct ggaggagacc acgaacaggg acgagaggtc ccagcggtcg gggtgctcgt
      181 ggaggcactc gacgagcggt cgggccatgg cgtcgccggt gatgacgatg gtggtgaccc
      241 tgtcgcgctc gatggtggcc cacacctcct cgggcgagaa cttgggcacg aacaccacgg
      301 tgttgccctc gaagaagaag cgcagcgcgc cccactgggc ggcgccgtgc atgagcggcg
      361 gggcgttgag cgtgaccatc ggagcctcgg acgccgccgc cttctcggcg agctgcatgc
      421 cgtgggtgac ccgctcgttg gtgtaggcgt cgacgccccc gccgagggcg tagaagacgt
      481 cctcgtggcg ccacacgacg cccttcggca tgccggtggt gccaccggtg tagagcacgt
      541 agtggtcgtc atcggtgcgg gggagctgcg ggcgctcggg gctggcgccg gcgatggcgt
      601 cggcccacga gacggcgccg atgcgggccg gatcgaggtc cgagccgtcg tcgatcatga
      661 cgaagtggcg cagcccgggg accttggcgg tgacggcctc gatgcggtcg atgtactcgc
      721 gggagtggac cagcgccacg aggtcggcgt tgtcgaacag gtaggccagc tcgtcttcga
      781 cgtagcggaa gttgatgttg atcggcacgg cgcggatctt gaaggccgcc accatggcga
      841 tcatccactc ggcggagttc tggccgtaga tgccgatgtg gtcgccgtgc ccgaagccct
      901 cggcgagcag gtggttcgcc agctggttcg cctcggcgtc gaactcggcg aaggtgagcg
      961 tgcggtcgcc gcacaccagg gcgcggcggt cgggaacggc gtcgacggcg cgctcgaaca
     1021 gatcagcgat gttgaacccc atggggtggt ttctaccacc cgaacgagaa caggtttcag
     1081 ttttcctgac gacccgtcag gtccacctcg cgtcgaactt ttcgtgactt tggtggctga
     1141 gccaccacca tcacgaaaag ttgcggggtt gggtcaggtg aggtcggcgc gcatgggggc
     1201 ggggttggcg ggcgggatgc cgtcggcgtc gaccggtgac ccgggatcca cggtgggctc
     1261 ggcgccctcc gcgaggagct tggcggcagc ctggagctcc tcgagcgccg gcttcacccg
     1321 atcggccatg tcccacacgt ccgacaccag ctcgcggtcg accatgaagc cgaagtccac
     1381 cgagccgcgg tagctcatga ccgtgatgtt gaggcccacg ccctccatga ccgggcccat
     1441 cgggtaggcc gccaccatct cggcaccggc gtagtagagc gggaagcccg ggccggggac
     1501 gttcgagatg atcacgttgt ggatcgggcg gtggctgtcg gccagacccc agctggagta
     1561 gagccgcgag gccaggttga acgtgttggg cgcggcgtac tcggcccagt tctggagcat
     1621 gtcggcgccg accgccttgt gctcctcctt ggcgccgacc gtgcaggcct ggatggcgcg
     1681 gatgcgctcg acggggtcgt cgagctgggt gtcgagcgtg gcgaacatgg ccgagacctt
     1741 gttgcccggc gccgcgtcct ccgcggcgcg caccgacacg gggcaggccg cgaccagcgg
     1801 ggtgtcgggc acgtcctcgt tggcttgcat gtaggaccgg agcgtgccgg cgcacaggcc
     1861 gagcacgacg tcgttgacgg tgcacccgaa cgcgtccttg agcgccttca cgtcgtcgag
     1921 cgtcacgcgg gcgaagccga ccgagcgcag cggcgacagg ctgccgttcc acgaggtgcg
     1981 gggcgcggtg agcggtgcgg caccggtctt gccctcctcg ctgctgcgcc ggccctgggc
     2041 gacgcggttg accgacacca ccgtgtcgcc gatgagcttg ggcagggcga tgagcttccg
     2101 cacccggctg gccgcggcgt ggccgagcag ctcgaagtcg ttggggatgt gctcgggctc
     2161 gtggtccgcg ggaggcgggg gctcggccgc ctccggctcc aggtcgaaca ggtggaccat
     2221 cagctcggcg ccggagctgc cgtcgatggc ggcgtggtgg accttggtga cgacgccgat
     2281 gcggtcctgc ttcaggccct cgatcaccca cagctcccag aggggacggg agcggtcgag
     2341 cggggtgctg gcgatctgac cggccatctc gccgagctcc cggcgcccgc cgggagccgg
     2401 gcagccgatc cgccggatgt ggaagtcgag gtcgaagttg gggtcctcca cccacagcgg
     2461 atgggccagg ttgaggggca cctcgcacaa ccgccgggtg aagggcggca ccaggtgcag
     2521 ccggccgcgg atgaactcct tcaggttctc gaacgagtag ccgccgggca cggtgctcgg
     2581 atccagcacc atcgtcatgg cgacgtgcat gtggttggtc gggttctcga ggtacaagaa
     2641 ggctgcgtca agcccggtca agcgttccaa ctgctgctcc cactcgtttt cggtccgggt
     2701 gcagtctgcc agcaaccggc cgcccgggac atcgaacggg gtcgcggccg gacctgagga
     2761 attcggagcc gcccgttctt gttgaagctt ctatgaccga tctcgccgaa tcccctgccc
     2821 gcgccatcgc cgacctcccc ggcggtccct acgacatcga gttcttcttc gacccgggct
     2881 gccccttcgc ctggcagacg tcggtgtggg tccggcgggt ggtggagctc cgtggcatca
     2941 aggtgggatg gcggttcatc tcgctgaagt tcatcaacga ggacaaggac gagtacatgc
     3001 cgggcatgcg cgacgcccag caccagggcc tgcgctacca ccggatctgc gccgccgccc
     3061 gcgagcagtt cggcaacggt gccgtcgccg gtctctacaa ggcgtggggc gaggcctact
     3121 ggaacaccga agccgaaggc gagttccccg agcggatcaa ggccggtttc gccaacgccg
     3181 tgcctgcgga cctcgtggcc tcggtcgggc tccccgagtc gatcctcgac gcggctgacg
     3241 acgagtcctg ggatgcgcag atccgagccg agagcgacga ggccttccgc cgcacgggcg
     3301 acgacgtcgg cacccccatc atcacctacc tcccgtcggg caactcgctg ttcggcccgg
     3361 tgatcagcag cgtccccgac gacgagaccg ccctcaagtt ctacgacgcc ctgcgggtct
     3421 tcgccgactt cccgcagttc tcggagctca agcgcaccaa gcgggccccg ctggacctcc
     3481 cgctcttcaa ctgagcggcc ggcccggagg gggtaccggg actagaacgt gttctagtct
     3541 ggggcggtgg acttcgatga cacgcctgaa gaggccgcct tccggaccga ggtccgggac
     3601 tggctggtgg cgcatgcccc ggccaagggc gggcccgacg acttctc
//
LOCUS       DATMFS010000215         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_53995, whole genome shotgun sequence.
ACCESSION   DATMFS010000215 DATMFS010000000
VERSION     DATMFS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53995"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..963)
                     /gene="tsf"
                     /locus_tag="VNQ33_03980"
     CDS             complement(166..963)
                     /gene="tsf"
                     /locus_tag="VNQ33_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWJ97293.1"
                     /translation="MPDFTAKDVQALRQSTGAGMMDAKKALQENAGDAEAAAKWLREK
                     GLAKSAAREDRDNSQGTVAVSLVDGVAAIAQLKCETDFVAKSDQFSDLVQEIATIVAT
                     QGTEAASKKSEAIDDLKVVLKENIELGEIVRFEAAEGNVIDTYVHRQDGRGVLGVIVE
                     LAGGGSAELAHDIAVHIAFSKPKYLTNDEVPEAEVAAERETLEAITKAEGKPEQAIAK
                     IVEGRIGAWFKDQVLIDQPYVKDDKQTIAQLLGGASIARFALVSIGS"
     gene            complement(956..1900)
                     /gene="rpsB"
                     /locus_tag="VNQ33_03985"
     CDS             complement(956..1900)
                     /gene="rpsB"
                     /locus_tag="VNQ33_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWJ97294.1"
                     /translation="MTEPVVTMKQLLEARVHFGHQTRRWNPKMKRFIFGERNGIYIID
                     LEQTLQRLETAYTFVRDTVADGGSILFVGTKKQAQDPIQSYAEKCGMPYINERWLGGM
                     LTNFETMSKRVAKMAEYRRMRDSGEFEAMPKKEALLISRELAKLERNLGGIAQMEKLP
                     SAVFILDTKEEHIAVTEANKLKLPIVAVVDTNCDPDIIQYVIPGNDDAIRSGALMCRV
                     IADAVEEGRLIASKRPGAAQPPKARSSEEEAAVAAQQAEARRQAAAAAAEREARIAAQ
                     QAAPQPAAEVVEAPEATPEAAEAAPESADPVEETTTDA"
     gene            complement(2115..>2565)
                     /locus_tag="VNQ33_03990"
     CDS             complement(2115..>2565)
                     /locus_tag="VNQ33_03990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWJ97295.1"
                     /translation="DGRGAVGGRRAPALRRGHDVYRPPTDAPVLDPFRPPPEPWLAGN
                     RGIEYATPAGAPVQAIGPGRVAFAGQVAGRLVVTVNHPDGLRSSYTGFGAITTRRNAV
                     VRSGDVVGTAGGPFHLGVRRGDTYLDPALLWGAAPGAGRVFLVSEHG"
BASE COUNT          314 a          860 c          953 g          438 t
ORIGIN      
        1 catgcggcgg gcgctgacga tctcctccgc cagctggcgg acgacctcac cggcgatgcc
       61 gtaggtgccg ccgccgaacg cctcaccgga cagcttgagc acgatccgcg accaccgtgt
      121 gggtgctccg ggcggggcgg gttccggaga ggcgtccgcc atgggtcagc tcccgatcga
      181 gaccagcgcg aagcgggcga tggacgcccc gcccagcagc tgggcgatgg tctgcttgtc
      241 gtctttgacg tagggctggt cgatgagcac ctggtctttg aaccaggcgc cgatgcggcc
      301 ctcgacgatc ttggcgatgg cctgctcggg cttgccctcg gccttcgtga tggcctcgag
      361 cgtctcgcgc tcggcagcca cctcggcctc ggggacctcg tcgttggtca ggtacttcgg
      421 cttggagaag gcgatgtgca cggcgatgtc gtgcgccagc tcggcggagc cgcccccggc
      481 gagctccacg atgaccccga gcacgccgcg gccgtcttgg cggtggacgt aggtgtcgat
      541 cacgttgccc tcggccgcct cgaaccggac gatctcgccc agctcgatgt tctccttcaa
      601 gacgaccttg aggtcgtcga tcgcctcgct cttcttcgag gcggcctccg tgccctgggt
      661 ggccacgatg gtggcgatct cctggacgag atccgagaac tggtcggact tggcgacgaa
      721 gtcggtctcg cacttgagct gggcgatggc ggcgacgccg tcgaccaggg acacggcgac
      781 ggtgccctgg ctgttgtcgc ggtcctcccg ggcggccgac ttggccaggc ccttctcacg
      841 gagccacttg gccgcagcct cggcatcgcc ggcgttctcc tggagcgcct tcttcgcgtc
      901 catcatcccg gcgccggtcg actggcggag ggcttggacg tccttggcgg tgaaatcagg
      961 catcggtggt ggtctcctcg accgggtcgg cggattcggg ggcggcctcg gcggcctcgg
     1021 gggtggcctc cggggcctcg acgacctcgg cggcgggctg cggcgccgcc tgctgggcgg
     1081 cgatgcgggc ctcgcgctcg gcggcggcgg ccgcggcctg gcgacgggcc tcggcctgct
     1141 gggcggcgac ggccgcctcc tcctcgctgc tgcgggcctt cggcggctgc gccgcgccgg
     1201 ggcgcttgga ggcgatgagg cggccctcct cgacggcgtc ggcgatcacg cggcacatga
     1261 gggcgccgga gcggatcgcg tcgtcgttgc ccgggatgac gtactggatg atgtcggggt
     1321 cgcagttggt gtcgacgacg gcgacgatcg gcagcttgag cttgttggcc tcggtgaccg
     1381 cgatgtgctc ttctttggtg tcgaggatga acacggcgga cgggagcttc tccatctggg
     1441 cgatgccgcc caggttgcgc tcgagcttgg ccagctcgcg gctgatgagg agggcttcct
     1501 tcttgggcat ggcctcgaac tcgccggagt cgcgcatgcg gcggtactcg gccatcttgg
     1561 cgacgcgctt ggacatggtc tcgaagttgg tgagcatgcc gccgagccag cgctcgttga
     1621 tgtagggcat gccgcacttc tcggcgtagg actggatggg gtcctgggcc tgcttcttgg
     1681 tgcccacgaa gaggatcgaa ccgccgtcgg cgaccgtgtc gcgcacgaag gtgtaggcgg
     1741 tctcgaggcg ctggagcgtc tgctcgagat cgatgatgta gatgccgttg cgctcaccga
     1801 agatgaagcg cttcatcttc gggttccagc gccgggtctg gtggccgaag tggactcggg
     1861 cctcgagcag ttgcttcatg gtcacgacgg gttccgtcac ggaccgctcc tctccaacgg
     1921 tttcggggac ctggccccaa gcgccaccac tgcgggaggc gaccgttggg aggggccggg
     1981 caatcggttt gggtatcggt ccaccgggcg atgggccggt tgacgagtgt agcgatgact
     2041 ggcacgcccg cccaaggtgg cgatgatggg gacctgctcg ccccgccccg gcgccggctg
     2101 cgcccggtgc ttgctcatcc gtgctccgag acgaggaaga cccggccggc tccgggcgcg
     2161 gcgccccaca ggagcgccgg gtcgaggtac gtgtcgccgc ggcgcacccc gaggtggaac
     2221 ggcccccctg ccgtgcccac cacatcaccg ctgcgcacca cggcgttccg gcgtgtcgtg
     2281 atggccccga agccggtgta ggaggagcgc agcccgtctg ggtggttcac ggtcacgacc
     2341 agacggcccg cgacctggcc ggcgaaggcc acccggccgg gaccgatcgc ctgcaccggc
     2401 gcacccgcgg gcgtggcgta ctcgatgccg cggttgccgg ccagccacgg ctccggcggc
     2461 ggccggaacg gatcgaggac cggggcgtcg gtcggagggc ggtagacgtc gtggccccgc
     2521 cggagcgccg gggcgcgcct ccctcccacc gcgcctctcc cgtct
//
LOCUS       DATMFS010000216         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_101809, whole genome shotgun sequence.
ACCESSION   DATMFS010000216 DATMFS010000000
VERSION     DATMFS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101809"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1248
                     /locus_tag="VNQ33_03995"
     CDS             295..1248
                     /locus_tag="VNQ33_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWJ97296.1"
                     /translation="MAAATLQTHPTGSSMIPPLAPEPGLRPDDEAFVADAARAGRSFS
                     PGLIAAIAAFADDPGAAGALLLRDVPIGTIPATPVTPTSPTGKDLRSEQVLLAVARLL
                     GEPVGYLPEHGGSIVQNLVPTKADVGRQTSTSSGVDLAFHTETAFHPFGPRYLLLLCL
                     RGDPGAATTLASVDDLLPGLRSETVETMRKPLFRTAVDESFGGQPGVPYGPARPVLGG
                     TDEQPWLCWDEALTTGTTPEAQAAHEELVAVVAQRRREIVLADGDLLIVDNTRCVHGR
                     RPFQARFDGTDRWLQRSFVVESLAPSVHDRTGRTITTQFAA"
     gene            complement(1398..2234)
                     /locus_tag="VNQ33_04000"
     CDS             complement(1398..2234)
                     /locus_tag="VNQ33_04000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWJ97297.1"
                     /translation="MSEDARLAAALWETGGWEATNIAAAVVAPGGAVATIGPGDRSFR
                     LASVTKLLTAYACLVAVEEGTLDLDEPTGPPGVTVRHLLAHAGGYGFDTGVLLLPERK
                     RIYSNTGFDALAEHLAHAAGMPADDYLVLAVFEPLGMDHTDVRGGSLAHGAWSTGADL
                     ARFAAELLQPTLIHPATLAEATTVAYPELAGIVPGIGPQNPNDWGLGFEIRGYKTPHW
                     TGRTNSAATFGHFGASGTFLWVDPAVQRSLLVLTDRNFGPWALEAWPPLADAVIAALR
                     PS"
     gene            complement(2227..>2451)
                     /locus_tag="VNQ33_04005"
     CDS             complement(2227..>2451)
                     /locus_tag="VNQ33_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HWJ97298.1"
                     /translation="RVGIAMNYSAGWIRQQENQQLGIPLETARTFGPRLRELCGFGVY
                     SGLIGHIDRRSPADVVLGAAPSDGAMVWDV"
BASE COUNT          322 a          925 c          882 g          322 t
ORIGIN      
        1 gccctgctcg ccgccctcgt ggccggcgtc tccgaggccc tgcgcgacga cgccaccgag
       61 gacctcgacc gggtcgagcg cacctggcag tccctcggcg ccctcaccga cggctgaccc
      121 gcacccgcgc ccgtcgaggg gcgagcgcac cggcgggtcg ctggtgcggc tgcccgcgct
      181 cacggatgag cggacggcaa gccatgtgca acaagtattt ctatacgatg ttaaagtgga
      241 acgaatgttc catccggcac cccgtgccgt cgcacccttc gaggaggatc ggccatggcc
      301 gccgcgacgc ttcagaccca cccgaccggg tcctcgatga tccccccgct cgcgcccgag
      361 cccggcctgc gccccgacga cgaggcgttc gtcgccgacg ccgcccgggc cgggcggtcg
      421 ttctcgcccg ggctcatcgc cgccatcgcc gcgttcgccg acgacccggg tgccgccggc
      481 gcgctgctgc tgcgcgacgt ccccatcggc accatccccg ccaccccggt gacgcccacg
      541 agcccgacgg gcaaggacct ccggtccgag caggtgctgc tggccgtcgc ccgcctcctg
      601 ggcgagccgg tgggctacct gccggagcac ggcggctcca tcgtgcagaa cctcgtgccc
      661 acgaaggccg acgtcggccg ccagacctcg acgtcgtcgg gcgtcgacct ggcgttccac
      721 accgagaccg ccttccaccc gttcggcccg cgctacctcc tgctcctgtg ccttcgcggc
      781 gatcctggag cggccaccac cttggcttcg gtcgacgatc tgctcccggg cctgcggtcc
      841 gagaccgtcg agaccatgcg caagccgctg tttcgcacgg cggtcgacga gtccttcggc
      901 ggccagcccg gcgtgcccta cggcccggcg cgtccggtcc tcggcggcac cgacgagcag
      961 ccctggctgt gctgggacga agcgctcacc accggcacca ccccggaggc ccaggcggcc
     1021 cacgaggagc tcgtcgccgt cgtcgcccaa cggcgtcggg agatcgtgct cgccgacggc
     1081 gacctcctga tcgtcgacaa cacccgctgc gtgcacggcc gccggccctt ccaggcccgc
     1141 ttcgacggca ccgaccgctg gctgcagcgc agcttcgtgg tcgagagcct ggccccctcg
     1201 gtccacgatc gaaccggccg caccatcacc acccagttcg ccgcctgatc ggccccccga
     1261 tcgcccctca ggggagtcgg gcaccgtcgg atcccgcccg ggtcggcctc ccacccgccc
     1321 gaacgccgac gcggcgtcgt gccgctcggt accggcacgc ccgcctcgcc ggtgcacccg
     1381 gaccggcggg cggggtgtca ggagggccgg agcgcggcga tgacggcgtc ggccagcggc
     1441 ggccaggctt cgagcgccca agggccgaag ttgcggtcgg tgaggacgag gaggctgcgc
     1501 tgcacggccg gatcgaccca gaggaacgtg cccgacgcgc cgaagtggcc gaaggtcgca
     1561 gcagagttgg tgcggcccgt ccagtgcggc gtcttgtagc cgcggatctc gaagccgagg
     1621 ccccagtcgt tcgggttctg cgggccgatg ccgggcacga tgccggcgag ctcgggatag
     1681 gcgacggtgg tcgcctcggc cagcgtcgcg ggatggatca gggtcggctg caggagctcg
     1741 gcggcgaacc gggcgaggtc ggcgccggtg gaccaggcgc cgtgagcgag cgagccgccg
     1801 cgcacgtcgg tgtggtccat cccgagcggc tcgaacacgg ccagcaccag gtagtcgtcg
     1861 gccggcatcc cggcggcgtg ggcgaggtgc tcggccaggg cgtcgaagcc ggtgttcgag
     1921 tagatccgct tccgctcggg gagcagcagc acgcccgtgt cgaagccgta gccgccggcg
     1981 tgggcgagca ggtggcgcac cgtcacgccg ggtgggccgg tgggctcgtc gaggtcgagc
     2041 gtgccctcct cgacggcgac gaggcaggcg taggccgtga ggagcttggt caccgaggcc
     2101 agccggaacg agcggtcgcc ggggccgatg gtggcgacgg cgccgccggg tgcgacgacg
     2161 gctgcggcga tgttggtggc ctcccaccct ccggtctccc agagcgcagc ggcgagtcgg
     2221 gcgtcttcag acatcccaga ccatcgctcc gtcggagggc gcggcgccga gcacgacgtc
     2281 ggcgggggag cggcggtcga tgtggccgat cagcccggag tacacgccga acccgcacag
     2341 ctcccgcagc cgcggcccga acgtgcgagc cgtctccagg gggatgccca gttgctggtt
     2401 ctcctgctgg cggatccagc ccgcgctgta gttcatggcg atgccgaccc g
//
LOCUS       DATMFS010000217         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_194528, whole genome shotgun sequence.
ACCESSION   DATMFS010000217 DATMFS010000000
VERSION     DATMFS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194528"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VNQ33_04010"
     CDS             <1..1137
                     /locus_tag="VNQ33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWJ97299.1"
                     /translation="SSGSVVDDDVLVGLAKHIHEASVPVAVWVGPSGSSALGGAAQLA
                     GAAERIGIAPGSRLGKTGPLVIPAEYLSAPFLAASDRLENGTINSKEAFTLKVTADKE
                     APVIGEFLIDLPGFEVKEVKQGKRVVSQPLTTPVFSSLPVQEQLFHTVSSPAAAYLLL
                     VIGLSLIIFEFFTAGVGVAGLVGAGCFVMSCYGLANLPVRTWALGLLVLAMFGFAVDV
                     QTGVPRVWSVIGVISLMVGSVFLFDGFDLSWITLLVGIVGVSMAMIAGMPAMVRTRFS
                     TPTIGREWMIGEEGEAVTNVSPDGVVQVRGALWRARTNRATPIDVHDGIRVVEVDGLL
                     LEVEPLEGGAVDYREKRRGGDDDEAPEEADDGRVLIDGKPLDPS"
     gene            1267..1527
                     /locus_tag="VNQ33_04015"
     CDS             1267..1527
                     /locus_tag="VNQ33_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWJ97300.1"
                     /translation="MSAQRSGEEWQLRAACRGPQATVFFPPGTFERKDEKLARESRAK
                     EICRTCPSKRPCLDYAISIREPHGIWGGLNEAERKSLMIQAV"
     gene            complement(1576..1977)
                     /locus_tag="VNQ33_04020"
     CDS             complement(1576..1977)
                     /locus_tag="VNQ33_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97301.1"
                     /translation="MAANVGRWWKYAQAKLNSVVSSSHAELDRLEADQAARTADKPWL
                     ASDGEAPTLEETRARIEWEAEEQRRQAEARAAADVEAADSPEDALAPASASAPPDPEV
                     VQARIELDRQARQSTERLEQIRKELGVDPPG"
BASE COUNT          266 a          725 c          763 g          361 t
ORIGIN      
        1 agctccgggt ccgtcgtcga cgacgacgtg ctggtcggcc tggcgaagca catccacgaa
       61 gcctcggtgc ccgtcgcggt gtgggtcgga cccagtggct cgagcgccct cggcggcgcc
      121 gcccagttgg cgggcgccgc cgagcgcatc ggcatcgccc ccggctcccg cctcggcaag
      181 accgggccgc tcgtgatccc cgccgagtac ctgtcggccc cgttcctcgc cgcgtccgac
      241 cggctcgaga acggcacgat caacagcaag gaggcgttca ccctgaaggt gacggccgac
      301 aaggaggcgc cggtcatcgg cgagttcctg atcgacctcc ccggcttcga ggtcaaggag
      361 gtcaagcagg gcaagcgcgt ggtgagccag ccgctcacga cgccggtctt ctcgtcgctc
      421 ccggtgcagg agcagctgtt ccacaccgtc tccagcccgg cggctgccta cctgctgctg
      481 gtcatcgggc tgtcgctcat catcttcgag ttcttcacgg ctggcgtcgg cgtggccggc
      541 ctggtcgggg ccggttgctt cgtgatgtcc tgctacgggc tggccaacct gcctgtccgg
      601 acgtgggcgc tcggcttgct ggtcctggcg atgttcggct tcgcggtcga cgtccagacg
      661 ggtgtgcccc gggtgtggtc cgtcatcggc gtgatcagcc tgatggtggg gtcggtgttc
      721 ctcttcgacg ggttcgacct gtcgtggatc accctcttgg tggggatcgt cggggtgtcg
      781 atggcgatga tcgccggcat gcccgccatg gtccggaccc ggttctcgac gccgaccatc
      841 gggcgggagt ggatgatcgg cgaggagggg gaggccgtca ccaacgtctc gcccgatggc
      901 gtggtgcagg tgcgcggggc gctgtggcgg gctcgcacca accgggccac gcccatcgac
      961 gtccacgacg gcatccgggt cgtcgaggtc gacggcctgc tgctcgaggt cgaacccctc
     1021 gagggcggtg cggtcgacta ccgcgagaag cggcggggcg gcgatgacga cgaggcgccc
     1081 gaggaggccg acgacggccg cgtcctcatc gacggcaagc cgctcgaccc cagctgacct
     1141 gcgcatctga cgccggcgcg atccctccgc gctcgcaaga gttcacttcc gcgctcgcga
     1201 tagttggggg ttgtgcccgc cccgaggcgg tcgtacggtc cggtttcacc gaaagggggc
     1261 tttctggtga gtgcacaacg atctggcgaa gagtggcagc tgcgggctgc gtgcaggggt
     1321 ccgcaagcga ccgtgttctt ccctccgggg acgttcgagc gcaaggacga gaagctggcg
     1381 cgcgagagca gggcgaagga gatctgtcgg acctgtccgt cgaagcgccc gtgcctcgac
     1441 tacgccatct cgatccgtga accgcacggc atctggggcg ggctcaacga ggccgagcgc
     1501 aagagcttga tgatccaagc cgtctgaggc gctgtccgtc tgaggcgctg cctcatctcg
     1561 gtggcgggcg ggcggtcaac ccggcgggtc gactcccagc tccttgcgga tctgctcgag
     1621 gcgctccgtc gactggcgcg cctggcggtc gagctcgatc cgagcctgga cgacctcggg
     1681 gtccggcgga gccgatgccg acgccggcgc gagcgcgtcc tcgggcgagt cggcggcctc
     1741 gacgtcggcc gcggcgcgag cttcggcctg ccgccgctgc tcctcggcct cccactcgat
     1801 gcgggctcgg gtctcctcca gcgtgggcgc ctcgccgtcg ctcgcgagcc acggcttgtc
     1861 ggcggtccgc gccgcctgat cggcctcgag ccggtcgagc tccgcgtggc tggagctcac
     1921 cacgctgttg agcttcgcct gggcgtactt ccaccaccgg ccgacgttgg ctgccatggc
     1981 gccagtctcg cgtcgatcag tcgtggtcgc cgcggtcctt gcggacggcc gccttcttgc
     2041 cccggatcgt ggtggccttc atggcatcga tgacccggtc gaccgagtcg gtcgggacct
     2101 cgaccagggc gaacc
//
LOCUS       DATMFS010000218         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_147848, whole genome shotgun sequence.
ACCESSION   DATMFS010000218 DATMFS010000000
VERSION     DATMFS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147848"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..646)
                     /locus_tag="VNQ33_04025"
     CDS             complement(191..646)
                     /locus_tag="VNQ33_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97302.1"
                     /translation="MVVAAYQDTGYNVMLLLHILAVIVAFAPAFVWPFASVKLKKEGK
                     PVGPAIGALAAGNTAKIHGPALVLVGLFGFGLVGMSDKYIKFDQSWVMAAMVVWFLML
                     GIIFGMMLPAEKKSAGGDESADKLVSMAGGIMHLLLLVMLYLMVFKPGL"
     gene            757..1245
                     /locus_tag="VNQ33_04030"
     CDS             757..1245
                     /locus_tag="VNQ33_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97303.1"
                     /translation="MVTQVAEDVKLTAVGGRTATIGEWTTTFQLLTVVIDPYTHESAW
                     LLETAGRVLANFRGADCRVAWTVTADERDAKRFLGPWAEEFLTFADPDRELVKALELE
                     RLPALAYIRQDLAIVGTAQGWKPDEWEHLGALVGKVNSWSHPNLPGNGDPGPFEGSPA
                     LG"
     gene            complement(1258..>2492)
                     /locus_tag="VNQ33_04035"
     CDS             complement(1258..>2492)
                     /locus_tag="VNQ33_04035"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWJ97304.1"
                     /translation="AKSKEVPSVDDKLGDAIRKAAEVVATGAHDDQFPIDVFQTGSGT
                     SSNMNANEVIARLADLSLGRTGKAKVSPNDHVNASQSSNDVFPTAIHLAATEAVVTDL
                     IPALEHLADALRSKADELAGVVKAGRTHLMDAVPITLGQELGGYATQVTQSVRRLRST
                     LPRLGELPIGGTAVGTGLNTPAGFGADVVKRLAKRTGLPLTEAADHIAAQSSRDGLVE
                     ASGQVRVAAVALIKIANDLRWMASGPNTGLAEIHLPDLQPGSSIMPGKVNPVLCEAVT
                     QVGAQVIGNDAAIAFSGSQGNFELNVFMPVMARNLLESIELVANVSRLFADRCIAGIT
                     ADVERCRANAESSPAVATSLNPYLGYERTAQLIKESQKTGTPIRELVTATGEVSDEDL
                     DRALDVTALTKGGLPPTP"
BASE COUNT          364 a          858 c          894 g          376 t
ORIGIN      
        1 ccggcccgtt ggccgggtgg cggtcgatca gccggcggca ggcggtgacg agcgagaccg
       61 ggtcgtcggc gaacgacgcc agcgaggccg ccgcctcctg caccacgtcg tcgggcggcg
      121 cgcccgtgga gcgggccacg aaccgcaggc gctcgatcgg gtgcacgacc gcgacgctat
      181 cggcgggtga tcagagtccg ggcttgaaga ccatcaggta gagcatcacg agcaggagca
      241 ggtgcatgat cccgcccgcc atcgagacga gcttgtccgc gctctcgtcg ccaccggccg
      301 acttcttctc ggcgggcagc atcatcccga agatgatgcc gagcatcagg aaccacacga
      361 ccatcgccgc catgacccac gactggtcga acttgatgta cttgtcgctc atcccgacca
      421 ggccgaaccc gaagaggccc acgaggacca gggccggccc gtggatcttg gcggtgttgc
      481 cggcggcgag ggcgccgatg gcgggaccga ccggcttgcc ctccttcttc agcttcaccg
      541 aggcgaaggg ccacacgaac gcgggagcga aggcgacgat caccgcgagg atgtgcagca
      601 ggagcatgac gttgtagccc gtgtcttggt acgcggcgac gaccatgggg ttccctccgg
      661 gggttcggtt ccagggcgaa cctagtccga gacccctgac cgagacgcca gacccgaatc
      721 tgcttcgcgc caggcgcacc aactaccgtt cccgccgtgg tcacgcaggt tgcagaagac
      781 gtcaagctca ccgccgtcgg ggggcgcacc gccaccatcg gagagtggac caccaccttc
      841 cagctgctga ccgtcgtcat cgacccgtac acgcacgaga gcgcctggct gctggagacc
      901 gcaggccgcg tgctggccaa cttccgcggc gccgactgcc gggtggcctg gaccgtgacc
      961 gccgacgagc gcgacgccaa gcggttcctc gggccgtggg ccgaggagtt cttgaccttc
     1021 gccgatcccg accgggagct ggtgaaggcc ctcgagctgg agcggctccc cgccctcgcc
     1081 tacatccgcc aggacctggc catcgtgggc accgcccagg gctggaagcc cgacgagtgg
     1141 gagcacctcg gtgccctcgt cggcaaggtg aactcgtgga gccaccccaa cctgccgggc
     1201 aacggcgacc cgggcccctt cgagggctcc cccgccctcg gctgatcgcc cgccccctca
     1261 gggggtcggc ggcaacccgc ccttcgtgag agccgtcaca tcgagggccc gatcgaggtc
     1321 ctcgtcggag acctcccccg tggccgtcac cagctcgcgg atcggggtcc ccgtcttctg
     1381 gctctccttg atgagctgcg ccgtgcgctc gtagccgagg tacgggttga gcgaggtggc
     1441 gacggccggg ctggactcgg cgttggcccg gcagcgctcg acgtctgcgg tgatgccggc
     1501 gatgcagcgg tccgcgaaga gccggctgac gttggccacc agctcgatgg actcgagcag
     1561 gttccgggcc atcaccggca tgaacacgtt gagctcgaag ttgccctgcg agccggagaa
     1621 ggcgatggca gcgtcgttgc cgatgacctg ggcgccgacc tgggtgacgg cctcgcacag
     1681 gaccgggttg accttgccgg gcatgatcga cgagccgggc tggaggtcgg gcagatggat
     1741 ctcggccagg cccgtgttgg gtccgctggc catccagcgc aggtcgttgg cgatcttgat
     1801 gagggccacg gcggcgacgc ggacctggcc cgatgcctcg accagcccgt cccgggacga
     1861 ctgggcggcg atgtggtcgg cggcctcggt gagcggcagg ccggtgcgct tggccagccg
     1921 cttcacgaca tcggcgccga agcccgccgg cgtgttcagc ccggtgccga ccgcggtgcc
     1981 cccgatgggc agctcgccca ggcgcggcag tgtgctgcgc agccggcgga ccgactgggt
     2041 cacctgggtg gcgtagccgc cgagctcctg gccgagcgtg atcggcaccg cgtccatcag
     2101 gtgcgtccga ccggccttca ccacaccggc gagctcgtcg gccttcgacc gcagggcgtc
     2161 ggccaggtgc tcgagcgccg ggatgaggtc ggtgacgacg gcctcggtgg cggccaggtg
     2221 gatcgccgtc gggaacacgt cgttggagga ctggctggcg ttgacgtggt cgttcgggct
     2281 gaccttcgcc ttgcccgtgc gcccgaggct gaggtcggcg aggcgggcga tgacctcgtt
     2341 ggcgttcatg ttcgacgacg tgcccgagcc ggtctggaag acgtcgatcg ggaactggtc
     2401 gtcgtgcgcc ccggtggcca ccacctcggc ggccttgcgg atggcgtcgc cgagcttgtc
     2461 gtcgaccgac ggcacctcct tgctcttggc gt
//
LOCUS       DATMFS010000219         3703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_77983, whole genome shotgun sequence.
ACCESSION   DATMFS010000219 DATMFS010000000
VERSION     DATMFS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77983"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /gene="murJ"
                     /locus_tag="VNQ33_04040"
     CDS             <1..1940
                     /gene="murJ"
                     /locus_tag="VNQ33_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWJ97305.1"
                     /translation="TFDEENDPAPVWQPPEQRRDRPDRPTVVRPRTISALGAPEPLPP
                     AVPWLSPPLESFSDLVWQPPTGPGATTLLDPDPDIWVLDDAPVPPPVGGAATAEETAP
                     AVGLNDDPRHLATEAKASSAKGLLQANLLVASGTAVSRLTGLVRTSLILVALVGGLND
                     AYNGANNTPNMIYELILGGVLTASLVPLFTDDMERGDGQDGTSAIISFALVALVIVSL
                     LALIVGPALMHLFATGAPKANHDVYLEVGVPLSLLFAPQVFFYGLMAVWSAVLNSRHR
                     FMAAAWAPVLNNVIAIATLVLAVKVAGGNPSLRDAAADRRLIWILGVGTTLGIVVMAL
                     SLYPAIRRSGVRLKFKVKMRHPAVRRAIRLSGWTFGYVIANLIAVVVIQILAKPSTGD
                     PTRYTVAYQYFQLPHALLAVSIMTTFEPLLGRADSRGDRSLFNSQLLLGFRLTALLII
                     PAAVGYLALPKGLDARTFTATGDLGKALGLSGIIAAFAFGLPGFSTYLYGLRGFYAMK
                     DTRTPFIINCGENAINIAAAVVFARWWGVVGLALSFSLAYTVAAAVTVVVLARRSPGF
                     DWRGLAHTWLKLVVAAGIMGGFVYGVVSSLAPSSAAMLVPTVLAGVVVGIVTYFASIY
                     ALRVPGISELMTRLPGLRRFA"
     gene            1995..2813
                     /locus_tag="VNQ33_04045"
     CDS             1995..2813
                     /locus_tag="VNQ33_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HWJ97306.1"
                     /translation="MIKLFKRWWAYLTAGANQKFNENADPKIQLEQAITEAQDQHRRL
                     KEHAANVIAQQHLTEQQLDRQLAQLEKLNTNARQAVKMADDATKKGDAAKAAEYTRAA
                     ETIATQLIAVETQIADLKEQHYAATQAADQAKAAVATNGQVLQQKLAEKQKLLSQLEQ
                     AKMQEQMNKAMASLSETVGQEVPTLNEVRDKIEQRYAKAKGMSELQETSVETRMVEIE
                     AAAQNVEAQARLDEIRSSMGIDTAAAPAEAPAAPETATATEPPAEPTAQPSPGV"
     gene            complement(2815..>3703)
                     /gene="proB"
                     /locus_tag="VNQ33_04050"
     CDS             complement(2815..>3703)
                     /gene="proB"
                     /locus_tag="VNQ33_04050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWJ97307.1"
                     /translation="LQAVSAIGQSRLMRVYDECFAGHGLVAGQVLLAPLDFGIRQQYL
                     HARGTLERLLDLGVVPVINENDAVADDEIRFGDNDRIGALVAQLIGADLLVLLTDAPG
                     LLTADPRIDAQASLIEEVLEVTADLERVAGGAGSARGSGGMSSKVAAAKMASWSGVRA
                     VIAAAHREGVLADAVAGTSGVGTVVQPRSQRISARKLWIAFALESGGRVTVDEGARRA
                     LLERGVSLLPAGVTGVSGGFVADQGVEIEGPDGVVFAKGLTRLSALELSEVSGRRTGD
                     LPDGMPHEVVHRDDLVIVP"
BASE COUNT          571 a         1415 c         1192 g          525 t
ORIGIN      
        1 tgaccttcga cgaggagaac gaccccgcgc ccgtgtggca gcccccggag cagcgccgcg
       61 accggcccga ccgccccacc gtcgtccggc cccggaccat ctcggccctc ggcgcgcccg
      121 aaccgctccc cccggcggtg ccctggctca gcccaccgct cgagtcgttc tccgacctgg
      181 tctggcagcc gcccacgggc ccgggggcga cgaccctcct cgatcccgat cctgacatct
      241 gggtgctcga cgatgctccg gtccccccgc cggtcggtgg tgcggcgacc gcggaggaga
      301 cggctcccgc cgtcgggctc aacgacgatc cccgccacct ggcgaccgag gccaaggcgt
      361 cgtcggcgaa gggcctgctg caggccaacc tcctcgtggc cagcggcacg gccgtctccc
      421 ggctcaccgg gctggtccgc accagcctga tcctggtggc gctcgtcggc gggctgaacg
      481 acgcctacaa cggggcgaac aacaccccca acatgatcta cgagctcatc ttgggcgggg
      541 tcctcaccgc gtcgctcgtc ccgctgttca ccgacgacat ggaacgaggc gacggccagg
      601 acggcacgtc ggcgatcatc tcgttcgcac tggtcgcgct ggtgatcgtc tcgctgctgg
      661 cgctcatcgt cggccccgcc ctcatgcacc tgttcgccac cggcgccccg aaggcgaacc
      721 acgacgtcta cctcgaggtc ggggtgccgc tgtcgctcct gttcgccccc caggtgttct
      781 tctacggtct catggcggtc tggtcagcgg tcttgaactc acgccaccgg ttcatggccg
      841 cggcgtgggc cccggtgctc aacaacgtca tcgccatcgc caccctcgtc ctcgccgtca
      901 aggtggccgg cggcaacccc agcctccgcg acgcggccgc tgaccggcgg ctcatctgga
      961 tcctcggcgt cgggaccacg ctcggcatcg tcgtgatggc gctctcgctg tatccggcca
     1021 tccggcgctc cggcgtccgg ctcaagttca aggtgaagat gcgccatccc gcggtgcgcc
     1081 gggcgatccg cctgtccggc tggaccttcg gctacgtcat cgccaacctg atcgccgtcg
     1141 tggtcatcca gatcctggcc aagcccagca ccggtgaccc cacccgctac acggtcgcct
     1201 accagtactt ccaactcccc cacgccctcc tcgccgtgtc gatcatgacc accttcgagc
     1261 ccctcctcgg ccgagccgac tcccgcgggg accggtcgct cttcaacagc cagctgctcc
     1321 tcggcttccg actgaccgcg ctcttgatca tcccggccgc ggtgggctac ctcgccctcc
     1381 ccaagggcct cgacgcccgg accttcacgg ccaccggcga cctcggcaag gccctcgggc
     1441 tcagcgggat catcgccgcg ttcgccttcg ggctccccgg cttctcgacc tacctgtacg
     1501 ggctccgtgg cttctacgcg atgaaggaca cgaggacccc gttcatcatc aactgcggtg
     1561 agaacgccat caacatcgcg gccgccgtcg tgttcgcccg ctggtggggc gtcgtggggc
     1621 tcgccctctc gttctcgctc gcctacaccg tggccgccgc cgtcaccgtc gtggtcctcg
     1681 cccgccggag tcccggcttc gactggcgcg gcctggccca cacctggctc aagctcgtcg
     1741 tcgccgccgg catcatgggc gggttcgtgt acggcgtcgt ctcctccctg gctccgtcgt
     1801 ccgccgcgat gctcgtcccg acggtcctcg ccggcgtcgt ggtgggcatc gtcacgtact
     1861 tcgcgtcgat ctacgccctc cgcgtgccgg gcatctcgga gctcatgacc cggctgcccg
     1921 gcctgcgacg cttcgcctga cgcgctcacg gccacccgac cccacgcccg tctcggtgcc
     1981 ataggctgcg gcgcatgatc aagctcttca agcgctggtg ggcctacctg accgctggtg
     2041 cgaaccagaa gttcaacgag aacgccgatc ccaagatcca gctcgagcag gccatcaccg
     2101 aagcccagga ccagcaccga cggctgaagg agcacgcggc caacgtcatc gcccagcagc
     2161 acctgaccga gcagcagctc gaccgccagc tggcccagct cgagaagctg aacaccaacg
     2221 cccgccaggc cgtgaagatg gccgacgacg ccaccaagaa gggcgacgcg gccaaagcgg
     2281 ccgagtacac gcgggcggcc gagaccatcg ccacgcagct catcgccgtc gagacccaga
     2341 tcgccgatct gaaggagcag cactacgccg ccacccaggc cgccgatcag gccaaggccg
     2401 ccgtcgccac caacggccag gtcctgcagc agaagctggc ggagaagcag aagctcctca
     2461 gccagctcga gcaggccaag atgcaagagc agatgaacaa ggccatggcc tccctctcgg
     2521 agaccgtcgg ccaggaggtc ccgaccctca acgaggtccg cgacaagatc gagcagcgct
     2581 acgccaaggc gaagggcatg agcgagctgc aggagacctc ggtggagacc cgcatggtcg
     2641 agatcgaggc cgccgcccaa aacgtcgagg cccaggcccg cttggacgag atccgcagct
     2701 cgatgggcat cgacacggcg gcggctcccg ccgaagcgcc ggccgcaccc gagacggcga
     2761 cggccaccga gccgccggcc gagcctacgg cccagccctc gcccggggtc tgagctaggg
     2821 gacgatcacg aggtcgtcgc ggtggacgac ctcgtgcggc atgccatcgg gcaggtctcc
     2881 ggtgcggcgg cctgacacct ccgacagctc gagtgcgctg aggcgcgtca ggcccttggc
     2941 gaacacgacc ccgtcgggtc cctcgatctc gacgccctga tcggcgacga agccgccgga
     3001 cacgccggtg acgcccgccg gcagaaggga gacgccccgc tcgagcaggg cacggcgggc
     3061 accctcgtcg acggtgacgc ggccaccgga ctccagggcg aacgcgatcc agagcttgcg
     3121 ggcgctgatg cgctgggatc ggggctgcac cacggtcccc acacccgagg tgccggcgac
     3181 ggcatcggcc agcacgccct cgcggtgcgc ggcggcgatg accgcccgga caccggacca
     3241 cgatgccatc ttggcggccg ccaccttgga ggacatgccg ccgctgcccc gcgcgctgcc
     3301 ggcgcccccg gcgacgcgct ccagatcggc ggtgacctcg aggacctctt cgatgagcga
     3361 cgcctgggcg tcgatgcggg gatcagcggt gagcaggccc ggtgcatcgg tcaacagcac
     3421 gaggaggtcg gcaccgatga gctgggcgac gagggccccg atgcggtcgt tgtcgccgaa
     3481 gcggatctcg tcgtcggcga cggcgtcgtt ctcgttgatg accggcacca cccccagatc
     3541 gaggaggcgt tcgagggtgc cccgagcgtg gaggtactgc tggcggatgc cgaagtcgag
     3601 cggtgccagc agcacctgac cggcgacgag gccgtgaccg gcgaagcact cgtcgtagac
     3661 tcgcatgagc cggctctggc cgatggccga gaccgcctgg agc
//
LOCUS       DATMFS010000220         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_78069, whole genome shotgun sequence.
ACCESSION   DATMFS010000220 DATMFS010000000
VERSION     DATMFS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78069"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VNQ33_04055"
     CDS             complement(<1..1461)
                     /locus_tag="VNQ33_04055"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97308.1"
                     /translation="MELDLAAIHERLAAELGDRPCLIWRGQTWSWTDVTDRSRRLANV
                     FVEHGITRRTSLAGCAGWESPHDHVGLYLHNGNAYLEAQLGAAKAAAAAFNVNYRYVA
                     DELAYLFLDSNAAAIVYQGCFAATLADVLPQLRRQPLLLRVDDGSGDELLPGALDYEE
                     ALASSSPEPPDVATSGDDLYMLYTGGTTGNPKGVLWRQADFLVAALGVRRKDGTEHRS
                     LEELVEPAVRRNLATLPAPPLMHGAAMWNALSTWINGGTIVVQDRVDRMDPADILDCC
                     ERHQVSSLLIVGDAFARPLIDEQRRQPRDLSALRFVLTGGAILSPSLRADVLDLVPQV
                     RIVDVLGSSESGRQAVASTVAGDEIGPTRFAPSATAAVLDDGLTRRLEPGDGEIGWLA
                     QGGRIPRGYRGDEAKTASTFPVVDGGRWSVPGDRAALRADGSVELHGRESVTINTGGE
                     KVFAEEVEQVLKHHPAVYDVLVVGRPSPAWGQEVVAV"
     gene            complement(1471..1980)
                     /locus_tag="VNQ33_04060"
     CDS             complement(1471..1980)
                     /locus_tag="VNQ33_04060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ97309.1"
                     /translation="MQATLHTNRGDINLTLFDDSAPKTVANFAGLAKGEPTPSDQKAG
                     QSRTTPFYDGLVFHRVIDGFMIQGGCPDGSGRGGPGYKFEDEFGGPQDFNTPYKLAMA
                     NAGPGTNGSQFFITVGPTPHLLNRHTIFGEVTDAESRAVVDAIASTPVGRGDRPHDDV
                     VINSVTISD"
     gene            2037..2675
                     /locus_tag="VNQ33_04065"
     CDS             2037..2675
                     /locus_tag="VNQ33_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HWJ97310.1"
                     /translation="MTSDLDLRERAELCDLFERVGPDAPTLCGDWTTQDLAAHLVVRE
                     RDLLGSPGILIPWFAGETEKRMDHQIRRHGYPGLVDLVRSGPPFGPMRIPPVRSLANL
                     VEFYVHHEDVRRPAGEGPRTDRPDLDDQLWSLLGRMAPLMVRKAGVKGVRLVLRRDDG
                     QEHRAGRGDDVVITGPAGELVLELYGRRTVAQVAYDGPADAVARVRDAGFGI"
     gene            complement(2676..>3277)
                     /locus_tag="VNQ33_04070"
     CDS             complement(2676..>3277)
                     /locus_tag="VNQ33_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWJ97311.1"
                     /translation="GPRRRRGLGLTEPPPPPARRRRAPRLDPDTRRGLIADAAEQLLA
                     EHDPLLVTFDEVADAAGVSRALVHAYLGDRRGLIDAVQVQIVGRLDTWVGHGIRRGET
                     TPARLRAIVQGVLAFVEAEQDAWGVLGASGGFDHPALHGVRSRWASVLTADGDLDEVR
                     AQAAVSAVLGSAGSWVNRGIDPAEVEAALAPLLRGDRAG"
BASE COUNT          441 a         1140 c         1255 g          441 t
ORIGIN      
        1 gacggccacc acctcctggc cccacgccgg cgacggccgc cccacgacga gcacgtcgta
       61 gacggctggg tggtgcttga ggacctgctc gacctcctcg gcgaagacct tctcgccgcc
      121 ggtgttgatc gtcaccgact cccggccgtg gagctcgacg gacccgtcgg ctcggagggc
      181 cgctcggtcg ccggggaccg accaccgccc gccgtcgacg accgggaagg tcgatgccgt
      241 cttggcctcg tcgccgcggt acccgcgggg gatccgcccg ccctgggcga gccagccgat
      301 ctcgccgtca ccaggctcga ggcgccgggt gagaccgtcg tcgaggacgg ccgcggtggc
      361 ggagggggcg aagcgagtcg gtccgatctc gtcgccggcc acggtgctgg cgacggcctg
      421 gcgcccggat tccgatgacc cgaggacgtc gacgatgcgg acctggggca ccaagtcgag
      481 cacgtcggcg cgcagcgacg gggacaggat cgctccgccc gtgaggacga accggagggc
      541 gctcagatca cgcggctgcc ggcgctgctc gtcgatgagc gggcgggcga aggcgtcgcc
      601 gacgatcagc agcgagctca cctggtggcg ctcgcagcag tcgaggatgt cggccgggtc
      661 catgcggtcg acgcggtcct gcacgacgat ggtgccgccg ttgatccagg tgctgagcgc
      721 gttccacatg gcggcgccgt gcatgagcgg cggcgccggc agcgtggcca ggttccggcg
      781 cacggccggc tccaccagct cctcgagcga ccggtgctcg gtgccgtcct tgcggcggac
      841 gccgagggcc gccaccaaga agtcggcctg gcgccacagg acgcccttgg ggttccccgt
      901 ggtgccgccg gtgtagagca tgtagaggtc gtcgcccgag gtggcgacgt cgggcggctc
      961 cggggacgac gaggcgagcg cctcctcgta gtcgagggcg cccggcagca gctcgtcgcc
     1021 cgacccgtcg tcgacgcgga gcagcagcgg ctggcggcgg agctggggga gcacgtcggc
     1081 cagggtggcg gcgaagcagc cctggtacac gatggccgcc gcgttcgagt ccaagaacag
     1141 gtacgccagc tcgtcggcca cgtagcggta gttgacgttg aaggccgcgg ccgccgcctt
     1201 ggccgcgccg agctgggcct ccaggtaggc gttgccgttg tgcaggtaca gccccacgtg
     1261 gtcgtggggc gactcccacc cggcgcagcc tgcgagggac gtgcgccggg tgatgccgtg
     1321 ttcgacgaag acgttggcca gccggcgcga ccgatcggtc acgtcggtcc acgaccacgt
     1381 ctggccccgc cagatcaggc aggggcggtc accgagctcg gccgcgagcc gctcgtggat
     1441 ggccgccagg tcgagctcca ccggtgacgg tcagtcggag atggtgacgc tgttgatcac
     1501 cacgtcgtcg tggggacggt cgccgcggcc gaccggggtg gaggcgatgg cgtcgaccac
     1561 ggcgcggctc tcggcgtcgg tgacctcacc gaagatcgtg tggcggttga gcaggtgcgg
     1621 cgtggggccc accgtgatga agaactgcga gccgttggtg ccggggccgg cgttggccat
     1681 ggccagcttg tagggcgtgt tgaagtcctg cgggccgccg aactcgtcct cgaacttgta
     1741 gccggggccg ccgcggccgg agccgtcggg gcagccgccc tggatcatga acccgtcgat
     1801 gacgcggtgg aagaccaagc cgtcgtagaa cggggtcgtg cggctctggc cggccttctg
     1861 gtcgctcggg gtgggctcgc ccttggcgag gccggcgaag ttggccacgg tcttgggcgc
     1921 gctgtcgtcg aagagggtca ggttgatgtc gccacggttg gtgtgaaggg ttgcctgcat
     1981 ggtcggcatc ctgccacgtc gaccagccgg aacggacatg gcagtaccgt cagcgcatga
     2041 ccagcgacct ggacctccgt gagcgggccg agctgtgcga cctgttcgaa cgggtcgggc
     2101 ccgacgcgcc gaccctgtgc ggcgactgga ccacccagga cctcgccgca cacctcgtgg
     2161 tgcgcgagcg cgacctcctc ggctcgccgg gcatcctcat cccgtggttc gccggggaga
     2221 cggagaagcg gatggaccac cagatccgac gccatggcta cccgggcctg gtcgacctcg
     2281 tgcgctccgg tccgccgttc ggtccgatga ggatcccacc cgtccgctcg ctcgccaacc
     2341 tcgtcgagtt ctacgtgcac cacgaagacg tccgtcggcc ggcaggcgag ggaccgcgga
     2401 ccgatcgccc cgacctcgat gaccagctgt ggtcgctgct ggggcgcatg gcgcccctca
     2461 tggtccgcaa ggccggggtg aagggcgtcc gcttggtgct gcgccgcgac gacggccagg
     2521 agcaccgcgc cgggcgaggc gacgacgtcg tgatcaccgg accggcgggg gagctggtcc
     2581 tcgagctgta cggccggcgc accgtggccc aggtcgccta cgacggtccg gccgacgccg
     2641 tggcccgggt gcgagacgcc gggttcggga tctgatcagc cggcgcggtc gccgcgcagg
     2701 agcggggcca gcgccgcctc cacctcggcg gggtcgatgc cccggttcac ccacgagcca
     2761 gccgagccga gcacggcgct caccgcagct tgcgcccgga cctcgtcgag gtcgccgtcg
     2821 gcggtgagca cggaggccca ccgcgaccgc acgccgtgca gcgctggatg gtcgaacccg
     2881 ccgctggcgc cgagcacacc ccacgcatcc tgctccgcct cgacgaaggc gaggacgccc
     2941 tgcacgatgg cgcgcagacg cgccggcgtg gtctcgccac ggcggatgcc gtggcccacc
     3001 caggtgtcga ggcggcccac gatctgcacc tgcacggcgt cgatcaaccc tcggcggtcg
     3061 cccaggtagg cgtggacgag cgcccgcgac acgccggccg cgtcggccac ctcatcgaag
     3121 gtgaccagca gcggatcgtg ctcggcgagg agctgctcgg ccgcgtcggc gatgaggccg
     3181 cggcgcgtgt cggggtcgag ccgcggcgcc cggcggcggc gggcgggcgg cggcgggggc
     3241 tcggtcaggc cgaggccccg gcgccggcga gggccac
//
LOCUS       DATMFS010000221         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_263242, whole genome shotgun sequence.
ACCESSION   DATMFS010000221 DATMFS010000000
VERSION     DATMFS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263242"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..919
                     /gene="recO"
                     /locus_tag="VNQ33_04075"
     CDS             191..919
                     /gene="recO"
                     /locus_tag="VNQ33_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWJ97312.1"
                     /translation="MGTYRDSGVVLRTQKLGEADRIVTMLTRGRGVVRAVGRGVRKTS
                     SRFGGRLEPFMHVDVQFAEGRSLDVIAQVVTIDPFASHLGADYAAYTAGTAMLETAER
                     LVADTAEPSVPQYHLLVGALRALTEHRQPPGILLDSFQLRSLAIAGYAPSFDGCARCG
                     LEGPHRHFHAGSGGMLCDGCRVPGSSVPSATTVALLAALLVGDWSAVGLADERSRREA
                     GGIVANYLSWHLERGLRSLAHVDR"
     gene            919..1674
                     /locus_tag="VNQ33_04080"
     CDS             919..1674
                     /locus_tag="VNQ33_04080"
                     /inference="COORDINATES: protein motif:HMM:NF011404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWJ97313.1"
                     /translation="MPRNPTPHPSGATPPRLAPEQVPQHVAIVMDGNGRWAKERGLPR
                     TEGHKAGEAALFDVVEGAIEIGVKAVSAYAFSTENWKRSPDEVKFLMGFNRDVIRRRR
                     DEMHELGVRVRWAGRRPRLWRSVIRELEEAEQLTKDNTVCTLTTCVNYGGRAEIADAA
                     AALARDVKAGRLNPEKVTEATFARYLDEPWMPDVDLFWRTSGEQRTSNFLPWQSAYAE
                     LVFSDIAWPDVDRRALWAAVEEYATRQRRYGSA"
     gene            1842..2690
                     /locus_tag="VNQ33_04085"
     CDS             1842..2690
                     /locus_tag="VNQ33_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ97314.1"
                     /translation="MYDALVIGGGPAGLQAALTLGRMHRTVVLVDSGRYRNSAAEHVH
                     NFLTHDGRSSEELRKLAREELAGYATVETRDATVQSMRETAEGFVADGDDGPISARTV
                     VLATGMVDLLPDVPGLGEAWGAEVANCPFCHGHEFAERPVGVFGDGPHVDEVRSMLEP
                     IASEVITFRHDDLVEVERADGGLVAHRGPGDHVWLAGLFLHPGTKQAAPFAEQLGLEL
                     LPSGCVRIDALGRTNRPRVFAAGDLAHVEELPVPMPSLLAAAAAGLAAGSSAVRHLAA
                     SDPQDV"
BASE COUNT          383 a         1037 c         1000 g          388 t
ORIGIN      
        1 aactccagct tgcggacctt gttgccgccg aagccgaagc cgctgaggtc ctcgcgcttc
       61 atccagagtc cgtccacgtc ctgcaggggc gtgggccaca cgccgaggtg ggcgcggggg
      121 cgatcgctca ggtccatgga cccgagcgtg ccacaccggc ggacgaccgc gactggggca
      181 gaatggagcg gtgggcacct atcgcgactc cggtgtcgtc ctgcgcaccc agaagctggg
      241 ggaggccgac cgcatcgtca cgatgctcac gcgcggacgc ggcgtggtgc gggccgtggg
      301 tcggggggtc cgcaagacct ccagccgctt cggcggtcgc ctcgagccgt tcatgcacgt
      361 cgacgtgcag ttcgccgagg gccgctcgct cgacgtcatc gcccaggtcg tgacgatcga
      421 ccccttcgcg tcgcacctcg gcgccgacta cgccgcctac accgcgggca cggcgatgct
      481 cgagaccgcc gagcgcctcg tcgccgacac cgccgagccc tcggtcccgc agtaccacct
      541 gctggtgggg gcgctgcgcg cgctgaccga gcaccgccag ccgcccggca tcctgctcga
      601 ctcgttccag ctgcggtcgc tcgcgatcgc cggctacgca ccgtcgttcg acggctgcgc
      661 ccgctgcggc ctcgagggtc cgcaccgcca cttccacgcc ggctccggcg gcatgctgtg
      721 cgacggctgc cgcgtgcccg gctcctccgt cccgtcggcc accaccgtgg ccctgctcgc
      781 cgccctgctg gtgggcgact ggagcgcggt cggtctcgcc gacgagcggt cgcgccgcga
      841 ggccggcggc atcgtcgcca actacctgtc ctggcacctc gagcgcggcc tgcgctccct
      901 ggcccacgtg gatcgctgat gccccgcaac cccaccccgc acccgtcggg ggccaccccg
      961 ccgcgcctcg cccccgagca ggtgccccag cacgtcgcga tcgtcatgga cggcaacggg
     1021 cgttgggcga aggagcgtgg actgccgcgc accgagggcc acaaggccgg cgaggcggcg
     1081 ctcttcgacg tcgtcgaggg cgcgatcgag atcggcgtca aggccgtcag cgcctacgcc
     1141 ttcagcaccg agaactggaa gcggtcgccc gacgaggtga agttcctcat gggcttcaac
     1201 cgcgacgtca tccgccgccg ccgcgacgag atgcacgagc tcggcgtccg ggtgcgctgg
     1261 gccggccggc gcccgcggct gtggcgcagc gtcatccgcg agctggagga ggccgagcag
     1321 ctcacgaagg acaacaccgt ctgcaccctg acgacgtgcg tgaactacgg cggccgcgcc
     1381 gagatcgccg acgccgccgc ggcgctcgcc cgcgacgtca aggcgggccg gctgaacccc
     1441 gagaaggtca ccgaggccac cttcgcgcgc tacctcgatg agccctggat gcccgacgtc
     1501 gacctcttct ggcgcacctc gggggagcag cgcaccagca acttcctgcc gtggcagtcc
     1561 gcctacgccg agctggtgtt cagcgacatc gcgtggcccg acgtcgaccg ccgcgccctc
     1621 tgggccgcgg tcgaggagta cgccacgcga cagcgccgct acggctcggc ctgaccgcac
     1681 ccgcctccgg cggacgagat gtgtagcgct ctccaccttt tctcggccga atggccggat
     1741 tttcggtgga caacgcgaca catctggcgc cgcggagcga cgtgtcggcg gcatcgccct
     1801 gggtaccccc aagtgcaggc cacgacacgg agggagacgg gatgtacgac gcgctggtca
     1861 tcggcggggg accggcgggg ctgcaggccg ccctgaccct gggccgcatg caccggacgg
     1921 tggtgttggt cgactccggg cggtaccgca acagcgccgc cgagcacgtg cacaacttcc
     1981 tgacccacga cggacgctcc tcggaggagc tgcgcaagct cgcgcgcgag gagctggccg
     2041 ggtacgccac ggtcgagacg cgcgacgcca cggtgcagtc gatgcgagag acggcggagg
     2101 ggttcgtggc ggacggcgac gacggtccga tctccgctcg caccgtggta ctcgcgacgg
     2161 gcatggtcga cctgctgccg gacgtcccgg gcctcggcga ggcgtggggt gccgaggtgg
     2221 ccaactgccc cttctgccac ggacacgagt tcgccgaacg tccggtcggg gtgttcggcg
     2281 acggacctca cgtcgacgag gtgcggtcga tgctcgagcc gatcgcgtcg gaggtgatca
     2341 ccttccgcca cgacgacctc gtcgaggtcg agcgcgccga cggtggactc gtcgcgcacc
     2401 gagggccggg cgaccacgtg tggctggccg ggctcttcct gcatccgggc acgaagcagg
     2461 cggcaccgtt cgccgaacag ctcggcctgg agctcctacc gtcggggtgc gtccgcattg
     2521 acgcgctggg ccgcacgaac cggccccggg tcttcgcggc cggggacctg gcgcacgtcg
     2581 aggagctgcc cgtgccgatg ccgtcgctgc tggcggccgc ggccgcgggg ctggccgcgg
     2641 ggtcgagcgc cgtccgccac ctggcggcct ctgatccgca ggatgtgtag cgctctccac
     2701 cctttctcgg ccgaatggcc ggattatcgg tggacaccgc tacacatctc gcgccgtcag
     2761 acggtggcgg gcccctcgag cgaccagcgg gtcgtgcccg cgaggacg
//
LOCUS       DATMFS010000222         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_239664, whole genome shotgun sequence.
ACCESSION   DATMFS010000222 DATMFS010000000
VERSION     DATMFS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239664"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VNQ33_04090"
     CDS             complement(<1..1503)
                     /locus_tag="VNQ33_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ97315.1"
                     /translation="MSDAPVTVLGISALYHDAAAAIVVDGEVVAAAQQERFSRKKHDA
                     AFPLDAIRYCLKEAGVATDGLTAVAYYDKPLTTFVRILKSYVAAGPRGIRTFPKAMSE
                     WSTHKLWTSLEIERGIRTLGWDMPKDLYYAEHHISHAAAAFYPSPFERAAIVTMDGVG
                     EWATSSIGVGRGRTVELLREQRFPDSLGLLYSAFTYHCGFRVNSGEYKLMGLAPYGEP
                     VYVDRILEQLVDLADDGSLRMDLRYFDYLAGRQMTNRRFDDLFDGPARPPESPITQRE
                     CDLARSIQEVVERAVLGTARTARELTGERQLVLAGGVALNCVANGRLRAEGTFDDIWV
                     QPAAGDAGSALGCALWAYHEVLEKPRTPASPDAMHGTYLGPRYSGDDVARDLEAAGRP
                     FERITDPDVLADRVAALLADGQVVAVLQGRMEFGPRALGHRSILADPRDPEAQRTLNL
                     KVKQRESFRPFAPAVLAEHAEEWFDLDGPSPYMTFVAPVAEERWVAPEGEP"
     gene            complement(1500..1715)
                     /locus_tag="VNQ33_04095"
     CDS             complement(1500..1715)
                     /locus_tag="VNQ33_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HWJ97316.1"
                     /translation="MHAGPEDPGLSLPTRMGMRVRHTGRLGREVLHFGAQQRLWWFVP
                     VIVVLMVIALAATTTTAAVPVAVYTLF"
     gene            complement(1764..>2370)
                     /locus_tag="VNQ33_04100"
     CDS             complement(1764..>2370)
                     /locus_tag="VNQ33_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97317.1"
                     /translation="GVNEINTQSLVPEAVPSHYGITEDKEANDGKTFATRFGESTPSN
                     RELLADVWYAYSEHSLIHKVANWFSSRADARPATPTAADRPAPVAQAVPVEIDPTLQD
                     GRDAGAVYERIKQLTLSLSDRYDVPSLLYWQPFKSVGDIEQAATDQITPPTIDISTLL
                     LDHQDVFIDGAHTNEEGARIVAERIWKDLRPAVQTWHEEHP"
BASE COUNT          327 a          781 c          899 g          363 t
ORIGIN      
        1 cggctcgccc tcgggcgcga cccagcgctc ctcggccacg ggggcgacga aggtcatgta
       61 gggcgacggg ccgtcgaggt cgaaccactc ctcggcgtgt tcggccagga ccgccggtgc
      121 gaacggtcgg aagctctcgc gctgcttcac cttcaggttg agcgtccgct gggcctcggg
      181 gtcgcggggg tcggccagga tcgagcggtg gccgagggcc cgggggccga actccatccg
      241 gccctggagc acggcgacga cctggccgtc ggcgagcagc gccgccaccc ggtccgccaa
      301 gacgtccggg tcggtgatgc gctcgaaggg gcgtccggcc gcctccaggt cgcgggcgac
      361 gtcgtcaccg gagtagcggg ggccgaggta ggtcccgtgc atggcgtcgg gcgacgccgg
      421 ggtcctgggc ttctccaaga cctcgtggta cgcccagagg gcgcagccca gcgcgctgcc
      481 ggcgtcgccc gcggcgggct ggacccagat gtcgtcgaac gtgccctccg cgcgcaggcg
      541 gccgttggcc acgcagttca gcgccacgcc gccggcgagg acgagctggc gctcaccggt
      601 gagctccctg gcggtgcgcg ccgtacccag cacggcgcgc tcgacgacct cctggatcga
      661 gcgggccagg tcgcactcgc gctgggtgat gggcgactcg ggcggccggg ctgggccgtc
      721 gaacaggtcg tcgaagcgac ggttcgtcat ctgccggccg gcgaggtagt cgaagtagcg
      781 caggtccatc cgcagcgaac cgtcgtcggc gaggtcgacg agctgctcga ggatgcggtc
      841 cacgtagacc ggttcgccgt agggcgccag ccccatgagc ttgtactcgc ccgagttcac
      901 ccggaacccg cagtggtacg tgaacgcgga gtagagcagg ccgagcgagt ccgggaagcg
      961 ctgctcccgg agcagctcca cggtccggcc tcggcccacg ccgatggagc tggtggccca
     1021 ctcgccgacg ccgtccatgg tcacgatcgc ggcccgctcg aacggagacg ggtagaacgc
     1081 cgccgcggcg tggctgatgt ggtgctcggc gtagtacagg tccttcggca tgtcccagcc
     1141 gagggttcgg atgccccgct cgatctcgag gctcgtccag agcttgtgcg tcgaccactc
     1201 ggacatggcc ttcgggaagg tgcggatgcc gcgagggccg gcggcgacgt agctcttcag
     1261 gatgcggacg aacgtggtca gcggcttgtc gtagtaggcc accgcggtca gcccgtcggt
     1321 ggcgacgccg gcctccttga ggcagtagcg gatggcatcg agcgggaacg cggcgtcgtg
     1381 cttcttgcgg ctgaagcgct cctgctgggc cgccgcgacc acctcgccgt ccaccacgat
     1441 cgcggcagca gcgtcgtggt acagggcgct gatccccaag acggtgaccg gtgcgtcgct
     1501 caaaacagcg tgtagacggc gacgggcacc gcggcggtgg tggtggtggc ggccagcgcg
     1561 atcaccatga gcacgacgat cacgggcacg aaccaccaga gacgctgctg ggcaccgaag
     1621 tgcaggacct cacggcccag ccgacccgtg tggcggaccc tcatgcccat gcgggtcggc
     1681 agcgagagcc ccgggtcctc cggtccggca tgcatcaggc gagcgtagca aggccctgga
     1741 cgcgcttccg gcgcggcgct cggtcacggg tgctcctcgt gccacgtctg caccgcgggc
     1801 ctgaggtcct tccagatccg ctcggcgacg atccgggctc cctcctcgtt ggtgtgggcg
     1861 ccgtcgatga acacgtcctg gtggtcgagc aggagcgtgg agatgtcgat ggtcggcggg
     1921 gtgatctgat ccgtcgccgc ctgctcgatg tcgccgacgg acttgaacgg ctgccagtac
     1981 aggaggctgg gcacgtcgta gcgatcgctg agcgacaggg tcagctgctt gatccgctcg
     2041 tagaccgcac cggcgtccct gccgtcttgg agggtggggt cgatctcgac cggcacggcc
     2101 tgggccaccg gggcgggtcg gtcggcggcg gtcggggtcg cggggcgggc gtcggcccgg
     2161 ctggagaacc agttggccac cttgtggatc agcgagtgct cggagtaggc gtaccagacg
     2221 tcggcgagca gttcgcggtt gctcggcgtg gactcgccga agcgggtggc gaaggtcttg
     2281 ccgtcgttcg cctccttgtc ctcggtgatg ccgtagtgcg acggcacggc ctcggggacg
     2341 agcgattggg tgttgatctc gttgacgccg
//
LOCUS       DATMFS010000223         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_239712, whole genome shotgun sequence.
ACCESSION   DATMFS010000223 DATMFS010000000
VERSION     DATMFS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239712"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VNQ33_04105"
     CDS             <1..775
                     /locus_tag="VNQ33_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWJ97318.1"
                     /translation="TRPLTLTPLLTHEQPDLLVSHGRLGTFEWPGLRRGVVPFAVVDA
                     KANGPLGLASAGRRHLVALPAPLHVAPHPIETETRWPRARAVGFGTNEGSPIGDDLFR
                     SVRPYQSGDEQRRVHWKATAHHGQLMVRESDGLGVVIVRLVVDLGLPGPGADLAAGRA
                     AWVAAAALDRGWSVELTTLDASSEIPQLASLGRTFGPPPVLVDPPLVPLPTVTAPVRN
                     AKEVRRRLCTTACGTPVAPTGSGWRGLTCRVDRDGVHWS"
     gene            832..2388
                     /locus_tag="VNQ33_04110"
     CDS             832..2388
                     /locus_tag="VNQ33_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97319.1"
                     /translation="MDWPTEPLPADEPTRPRSQAAVYVVCALAMVIVCSAAETPTLGL
                     GCSLALAVGVGITLAAGLRRPFGGVPGAVVALIAMMAMAMLLAFTGGPPSAWMLVPVV
                     GAFVLGFDWHLVRRLRPLPFAFGFLVVLGLASGDRWTYPVGVLWLALALGALTSLESD
                     RRAAQPKVRAVSVGPEAPDVQATDLVTTVLVALAIALVAALVLSTPSCQPTDSSDLGP
                     SGSRSPYGTGSGSGLGSGSESGSGAGSGRTYVPDDDGRFLVPDDGSGSGSTSDIPSPD
                     LLPAPGESRRLLGDDGTTMFTQRGEDGAGAITVTDPDGTSRTYTYREQPDGTTRIQEL
                     DDDGRPGRTLTYDPKGQIATGGSGSDAGSGSAFDQQDREQEKEDDKPPSHLDWRLLLG
                     LVLLLAAGGALAWWWSKRPPEAPPVAAPPWALRLAREIDREGAARGPRRDRSQSLVRY
                     ADDLRAGPIPDDRVIEVAVTVSAALFGRHDPGPETQARAESTWAAVVEAHPAPGRAER
                     RRARAGTPTA"
     gene            complement(2480..>2942)
                     /locus_tag="VNQ33_04115"
     CDS             complement(2480..>2942)
                     /locus_tag="VNQ33_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97320.1"
                     /translation="INLRDPEERAWPASVGGRPEGLGGEKETAGEVTPSADPGVYIWN
                     SFDGWHLWVVGGEGIDGLTGTITSSDDVVGAVSSAPDAGTVSVEGKTITFDLAAGSAV
                     AGVDFDPGFAKELTFTLKTAGGDVAPAQVFTGASREPVDAVPVVIDKAVVD"
BASE COUNT          342 a         1184 c         1039 g          377 t
ORIGIN      
        1 gacccgaccg ctgaccctga cgccgctgct cacccacgag cagcccgacc tgctcgtcag
       61 ccacggccgc ctcggcacct tcgagtggcc gggcctgcgg cggggcgtgg tgcccttcgc
      121 cgtggtggac gccaaggcca acggaccgct cggcttggcc tccgccggcc gccgccacct
      181 cgtcgcgctg ccggcgccgt tgcacgtcgc gccccacccg atcgagaccg agacccggtg
      241 gccgcgcgcc cgcgccgtcg ggttcggcac gaacgagggc tcacccatcg gcgatgacct
      301 gttccggtcg gtgcgcccct accagtcggg cgacgagcag cgccgggtgc actggaaggc
      361 cacggcgcac cacggccagc tgatggtccg cgagtccgac ggcctgggcg tcgtcatcgt
      421 ccggctcgtg gtcgacctgg gcctccccgg cccgggcgcc gacctggcgg cgggccgcgc
      481 cgcgtgggtg gcggccgctg cgctcgaccg cggctggtcg gtcgagctca cgacgctcga
      541 tgcgtcgagc gagatccccc agttggccag cctcggacgc accttcggcc cgccgccggt
      601 gctcgtcgac ccgccgctcg tgcccctgcc caccgtcacc gcgccggtcc gcaacgccaa
      661 ggaggtgcgg cggcgcctgt gcaccaccgc gtgcggcacg ccggtggccc cgaccggcag
      721 cgggtggcgc gggctcacct gcagggtcga ccgcgacggg gtgcactggt cgtgaccacg
      781 ttccccggtc cgcccggccc cgccggtgca ccgcccggcc ccccgccccc gatggactgg
      841 ccgaccgaac cgctgccggc cgacgaaccg acccggccgc gcagccaggc ggcggtgtac
      901 gtggtgtgcg ccctggccat ggtgatcgtg tgctcggcgg cggagacgcc cacgctgggc
      961 ctcgggtgct ccctcgccct ggccgtcggc gtcggcatca ccctcgcggc cggcctgcgc
     1021 cggccgttcg ggggcgtccc cggcgccgtc gtcgccctca tcgcgatgat ggcgatggcg
     1081 atgctgctcg ccttcacggg cggcccgccg tcggcgtgga tgctcgtacc ggtggtcggc
     1141 gccttcgtgc tcgggttcga ctggcacctc gtgcgccggc tgcggccgct cccgttcgcg
     1201 ttcgggttcc tcgtggtcct cggcctggcg agcggcgacc ggtggaccta tcccgtcggc
     1261 gtgctctggc tcgccctcgc gctcggcgca ctgacctcgc tggagtccga ccggcgcgcc
     1321 gcgcaaccca aggtccgggc ggtgagcgtc ggccccgagg cacccgacgt ccaggccacc
     1381 gacctcgtca ccaccgtcct cgtcgccctc gcgatcgcgc tcgtcgccgc cctcgtcttg
     1441 tcgaccccct cctgccagcc gacggactcc tccgacctcg gcccgtccgg ttcgaggtcc
     1501 ccgtacggaa cggggagcgg gtccgggctc ggatcgggat ccgagtcggg gtccggcgcc
     1561 ggatcgggcc gcacctacgt gcccgacgac gacgggcgct tcctcgtgcc cgacgacgga
     1621 tccggctcag gcagcaccag cgacatcccg agccccgacc tgctgccggc tcctggcgag
     1681 tcgcgccggc tgctcggcga cgacggtacg accatgttca cccagcgcgg cgaggacggc
     1741 gccggcgcca tcaccgtgac cgaccccgac ggcaccagcc gcacctacac gtaccgcgag
     1801 caacccgacg gcacgacccg catccaggag ctcgacgacg acggccggcc cggccgcacc
     1861 ctcacctacg acccgaaggg ccagatcgcg accggcggct ccggttcgga cgccggctcc
     1921 ggttcggcct tcgatcagca ggaccgggag caggagaaag aagacgacaa gccgccgtcc
     1981 cacctcgact ggcggctcct cctcgggctg gtgctgctgc tggcggctgg cggcgccctc
     2041 gcctggtggt ggagcaagcg gccgcccgaa gcgccgccgg tcgccgcccc gccctgggcc
     2101 ctccgcctgg cgcgcgagat cgaccgcgag ggagcggcgc gcggcccccg gcgcgaccgc
     2161 tcgcagtcgt tggtgcgcta cgccgacgac ctgcgagctg ggccgatccc cgacgaccgg
     2221 gtgatcgagg tggccgtgac cgtgtccgcc gcgctgttcg gccgccacga tcccggcccc
     2281 gagacccagg cccgggccga gtccacgtgg gctgcggtcg tcgaggccca cccggcgccg
     2341 ggccgagccg agcgccgccg agcacgcgcc ggcaccccga ccgcctgacc cgggacggtc
     2401 gcaccgagcc ccgcgaaccg gccggcggca cccggtccgg cgccgccgac gaggcaaggg
     2461 ccgccggtca ccgcagaggt cagtcgacca cggccttgtc gatgacgacg ggcaccgcat
     2521 ccaccggctc gcggctcgcc ccggtgaaca cctgggcagg cgccacatcg ccgccggcgg
     2581 tcttcagcgt gaaggtcagc tccttggcga agccggggtc gaagtcgacg cccgccacgg
     2641 cgctcccggc ggcgaggtcg aaggtgatcg tcttgccctc gaccgagacc gttccggcat
     2701 caggggcgga gctcacggcg ccgacgacgt cgtcgctgct cgtgatggtg ccggtgaggc
     2761 cgtcgatgcc ctcgccgccg acgacccaca ggtgccagcc gtcgaagctg ttccagatgt
     2821 acacaccggg atcggccgag ggggtgacct cgccggccgt ctccttctct ccgccgaggc
     2881 cctcgggccg gccgccgacc gaggccggcc acgcccgctc ctcgggatct cggaggttga
     2941 tg
//
LOCUS       DATMFS010000224         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_9322, whole genome shotgun sequence.
ACCESSION   DATMFS010000224 DATMFS010000000
VERSION     DATMFS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9322"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VNQ33_04120"
     CDS             complement(<1..544)
                     /locus_tag="VNQ33_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97321.1"
                     /translation="MLLLVTLGLLVATVSAIRGVWSPCGLSMLSSITPMTEAGRGNRF
                     STTARWFVLGGILGGLSLGLLAAGGAALVARADLGDTARWGIAAVAAIATTSIDLGAF
                     GIELPVFKRQVNDAWLRRYRGWVYGVGFGWQIGFGVATYIMTAGVFLTIALAVLGASP
                     AAALAIGATFGFVRGSAVHIG"
     gene            639..2006
                     /locus_tag="VNQ33_04125"
     CDS             639..2006
                     /locus_tag="VNQ33_04125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ97322.1"
                     /translation="MGGEPNASTASSRLRDLDTCAVLDAIPGVVAVVSADGEIVWINE
                     SMSEVTGYTVDDLVGTSMLDHLDLEWNPLSLESISYALDHPGMRLPTMLRFHTKHGPP
                     VVMEVTANNQIANPQICGLVAHLRPSDERQLLDLILESFASGDDLASTISRVHDVARA
                     ETLQAESAVFLVFSGGDDGSVLASSPEVAAISAIEGLSTPWSVAASSGRPVLLSDLAS
                     LPGPLRAAAEAAGFAACWSYPVCRTGTGVVDAVLVAWRRTTGAPEPTATMLAERLVRL
                     CALVLERVEHSRDLQHAADHDALTGLVNRSRFFASVDAHLAAERETLGVVYLDLDGFK
                     PINDRWGHAHGDRVLVAVADRLRASVRPGDTVARLGGDEFAIACPGAASDELVRLADR
                     LLAVVRAPITVDGEVLRVGASIGLASCAAGASPADVLVAAADSALLTAKANAKGTWQL
                     SETLA"
BASE COUNT          313 a          857 c          831 g          341 t
ORIGIN      
        1 ggccgatgtg caccgcgctg ccccggacga agccgaacgt ggcaccgatg gccaaggccg
       61 cggcggggct ggcgccgagc acggccaagg cgatggtgag gaagacgccg gccgtcatga
      121 tgtaggtggc gacgccgaag ccgatctgcc agccgaagcc gacgccgtac acccagcctc
      181 ggtagcggcg gagccacgca tcgttcacct ggcgcttgaa gacgggcagc tcgatgccga
      241 acgcgcccag gtcgatggac gtggtggcga tggctgcgac cgccgcgatc ccccaccggg
      301 ccgtgtcgcc caggtcggcg cgcgccacca gcgccgcgcc gccggcagcg aggaggccga
      361 ggctgagacc gccgaggatg ccgcccagca cgaaccagcg ggccgtggtg gagaagcggt
      421 tgccccgccc ggcctcggtc atcggggtga tggacgacag catcgagagg ccgcacggcg
      481 accacacgcc gcggatggcg gagacggtgg cgaccaggag acccagggtg acgagcagca
      541 gcatggtgac cccgatggta ccggcggcgg ggcacccgat ctcggccatc gggtgtgccg
      601 ccctcgatac cgtggccgcc gtgccgacag gaggggccat gggaggtgag ccgaacgcgt
      661 cgaccgcgtc ctcacgcctg cgcgatctcg acacctgcgc cgtcttggac gcgatccccg
      721 gtgtcgtggc cgtggtcagc gcggacggcg agatcgtctg gatcaacgag tcgatgtcgg
      781 aggtcacggg ctacacggtc gacgacctgg tcggcacgag catgctggac cacctggacc
      841 tcgagtggaa cccgctgtcg ctcgagtcga tcagctacgc gctcgaccat ccggggatgc
      901 gcctccccac catgttgcgg ttccacacga agcacgggcc gccggtggtg atggaggtca
      961 ccgccaacaa ccagatcgcc aacccgcaga tctgcggcct ggtggcgcac ctccggccga
     1021 gcgacgagcg gcagctcctc gacttgatcc tcgagtcgtt cgcgtccggc gacgacctcg
     1081 cttcgaccat cagccgggtc cacgacgtcg cccgggccga gacgctccag gctgagtccg
     1141 ccgtcttctt ggtgttctcg ggtggcgacg acggttcggt gctcgcctcg tcacccgaag
     1201 tcgccgccat cagcgcgatc gaggggctgt cgaccccgtg gtcggtggcg gcgtcgtcgg
     1261 gccggcccgt gctgctctcg gacctcgcgt cgctgccggg gccgctccga gccgccgctg
     1321 aggcggccgg gttcgcggcg tgctggtcct acccggtctg tcgcaccggt accggcgtgg
     1381 tcgacgccgt cttggtggcc tggcgccgta cgaccggcgc ccccgagccc acggccacga
     1441 tgctcgccga gcgcctcgtg aggctgtgcg ccctggtcct cgagcgcgtc gagcacagcc
     1501 gcgacctgca gcacgccgcc gaccacgacg ccctcaccgg gctcgtgaac cggtctcggt
     1561 tcttcgcatc cgtggacgcg caccttgccg cggagcggga gacgctgggc gtggtctacc
     1621 tcgatctcga cgggttcaag cccatcaacg accgctgggg gcacgcccac ggcgaccggg
     1681 tcctggtcgc cgtcgcggac cgcctccgtg cgtcggtgcg ccccggcgac accgtcgccc
     1741 gcttgggcgg cgacgagttc gccatcgcct gccccggtgc cgcgtccgac gagctggtcc
     1801 gcctggccga ccgcctgctc gccgtcgtgc gggcacccat cacggtggac ggcgaggtgc
     1861 tgcgggtcgg cgccagcatc ggcctggcct cctgcgccgc gggcgccagc cccgcagacg
     1921 tcctcgtcgc cgccgccgac tccgccctcc tgaccgccaa ggcgaacgcc aaaggcacct
     1981 ggcagctcag cgagaccctc gcctgagcgt cgccgcgtcg gggctccgcg cgtcgagccc
     2041 tcacggtcgg cgagcggcgg ccggtccgtt cgcgcgctga tggaccgcct tcgttgcatc
     2101 gtcaccgacg tcggtggtcg tccgtgcacc cggcgcctat ccgtggctga ccgcgggtgg
     2161 tgcggcccag tcggtaggtt tcgcccccgt gaccgttgcc gatcagctgc cgcgtgaccg
     2221 caacgcccga tcgtggtcga aggcgatctg gctctacctc gcgctcgtct tggtgtacca
     2281 ccggtccttc gagtggtcgt tccggagccc ggcgctgcag aactggtcga cgatcctggt
     2341 gt
//
LOCUS       DATMFS010000225         3483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_148067, whole genome shotgun sequence.
ACCESSION   DATMFS010000225 DATMFS010000000
VERSION     DATMFS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148067"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VNQ33_04130"
     CDS             complement(<1..674)
                     /locus_tag="VNQ33_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWJ97323.1"
                     /translation="MPWPTWQEAATASVVLVVLSLVLRRIDRRDAKVVAAFAQEVALV
                     SFLYMLWRLARQLPLLHEDGAIERGQWIYDVQKAVHLPSELSMEQWLAEHHWFAQVTV
                     DYYATVHVPALLCFLVWLWVRHRDQYPKWRNALAITTGFCLFIRFIRVSPPRLTPGHG
                     FWDLAAAMGRDIYGPVGTGASDQFAAMPSIHVAWACIVAFGIWHCAQSRWRIIGPIHL
                     VFTFWAV"
     gene            complement(799..2265)
                     /locus_tag="VNQ33_04135"
     CDS             complement(799..2265)
                     /locus_tag="VNQ33_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWJ97324.1"
                     /translation="MSPANQTMLARFAAATSIFALLMVGVVAVTAGDGNGSGEAAPAA
                     PVEVSLTEFAINPSAIVVPLGGSINVTNSGTMAHNVSVLDTPIKTPDLGAGESTKVDL
                     SSLEPGEYELFCAITGHKDSGMTATLTISDGSAAATAAAEETASASASEHADHAAADI
                     GSLEATDAMAKRIDKQMQDGMDAGVAAFLDNATKYAEGKISSGNQILEPKILPDGTKQ
                     FHLTAAVTDWEVSPGKVVKAWTYNGMVPGPWIKVDPNDDVEVVLTNNLPISTDIHFHG
                     VDVPNDQDGLAPITQDYIKPHTTYTYKFNVGPNNQQAMYHAHYHGQEAVLNGLFSIFQ
                     VGEVPIPPAGTYGQFQMPANVKVSQEIPMVLNDAGVIGLSLNGKAFPLTEPIVAEKDS
                     YVMLHYYNEGLVGHPMHLHRQPHIVIAKDGFALKAPYQVDTLWVSPGERYTVLIKASE
                     VGTWAFHCHILNHAESDNGLIGMVTAMVVPDPNAEKGT"
     gene            2407..3081
                     /locus_tag="VNQ33_04140"
     CDS             2407..3081
                     /locus_tag="VNQ33_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ97325.1"
                     /translation="MTIRVFLLDDHEVVRRGVRDLIESEDDLTVVGEASTEAEALERI
                     GLARPDVAVLDVQLEQGSGIEAAREIRSRHPEIRCVMLTSFADDEALVDSIMAGASGY
                     ILKQVRGHDLIEGVRKVGAGGSLLDPVMTSRVLDRLREGTEEDERTASLTPQERRILD
                     LLAEGLTNRQIGERLHLAEKTVKNYVSNLLMKLGMARRTEAAVYAARQADRQARAAHH
                     SSAGTR"
     gene            3113..>3483
                     /locus_tag="VNQ33_04145"
     CDS             3113..>3483
                     /locus_tag="VNQ33_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ97326.1"
                     /translation="MDDSTRPADLQRLIAAIASIGADLDLPVVLRRITEAATELVGAT
                     YGALGVLSPDGTHLSAFVTVGIDEETADEIGELPKGHGILGLLIVHPEPLRLDDLRQH
                     PESFGFPPNHPPMTSFLGVPI"
BASE COUNT          530 a         1169 c         1228 g          556 t
ORIGIN      
        1 accgcccaga acgtgaacac gaggtggatg ggcccgatga tccgccacct gctctgggcg
       61 cagtgccaga tgccgaaggc gacgatgcac gcccaggcca cgtggatgct cggcatggcc
      121 gcgaactggt ccgaggcgcc cgtccccacc ggcccgtaga tgtcacggcc catcgccgcg
      181 gccaggtccc agaacccgtg ccccggcgtg aggcgcggcg gtgagacccg gatgaaccgg
      241 atgaagaggc agaacccggt ggtgatggcc agcgcgttgc gccacttcgg gtactggtcg
      301 cggtggcgga cccacagcca cacgaggaag cagagcaggg ccggcacgtg gacggtggcg
      361 tagtagtcga cggtgacctg ggcgaaccag tggtgctccg ccagccactg ctccatcgac
      421 agctcgctcg gcaggtggac cgccttctgg acgtcgtaga tccactggcc gcgctcgatg
      481 gcgccgtcct cgtgcaggag ggggagctga cgggccaagc gccacagcat gtagaggaac
      541 gagaccagcg ccacctcctg ggcgaaggcg gccacgacct tggcgtcgcg ccggtcgatc
      601 cgccgcagca cgagcgacag gacgacgagc accacggatg cggtcgctgc ttcttgccag
      661 gttggccacg gcatggtgga gggcagcctg gcaagaccgg ccggtcacgg caaccccggg
      721 acgcgggaga tccccgggcg cgggcccggg gatctcggtg tgcggcgggt gggggtgtgc
      781 gaaccggccc gccgcaggtc aggtgccctt ctcggcgttc ggatccggga ccaccatcgc
      841 ggtcaccatg ccgatcaagc cgttgtcgct ctcggcgtgg ttgaggatgt ggcagtggaa
      901 ggcccaggtg ccgacctcgg acgccttgat cagcaccgtg taccgctcgc caggcgacac
      961 ccagagggtg tcgacctggt acggcgcctt gagggcgaac ccgtccttgg cgatcacgat
     1021 gtggggctgg cggtgcaggt gcatcgggtg ccccaccagg ccctcgttgt agtagtggag
     1081 catcacgtag ctgtcctttt cggccacgat cggctccgtg agcgggaagg ccttgccgtt
     1141 gagcgacagg ccgatcaccc cggcgtcgtt gagcaccatc gggatctcct ggctgacctt
     1201 cacgttggcg ggcatctgga actggccgta ggtgccggcg ggcgggatcg ggacctcgcc
     1261 gacctggaag atcgagaaca ggccgttcag gaccgcttct tgcccgtggt agtgggcgtg
     1321 gtacatcgcc tgctggttgt tcgggcccac gttgaacttg taggtgtagg tcgtgtgggg
     1381 cttgatgtag tcctgcgtga tgggggccag gccgtcttgg tcgttcggga cgtcgacgcc
     1441 gtggaagtgg atgtcggtgc tgatgggcag gttgttggtg agcacgacct cgacgtcgtc
     1501 gttggggtcc accttgatcc acggcccggg gaccatgccg ttgtaggtcc aggccttcac
     1561 gaccttgccg gggctgacct cccagtcggt gacggcggcg gtcaggtgga actgcttggt
     1621 gccgtccggg aggatcttcg gctcgagaat ctggttgccc gacgagatct tgccctcggc
     1681 gtacttggtg gcgttgtcca agaaggcggc gacgccggcg tccatgccgt cttgcatctg
     1741 cttgtcgatg cgcttggcca tggcatcggt ggcttcgagc gacccgatgt cggcggcggc
     1801 gtgatcggcg tgctcgctcg cgctggccga ggccgtctcc tccgcggcgg ctgtggccgc
     1861 ggcggagcca tcggagatgg tgagcgtggc ggtcatgccc gagtccttgt gaccggtgat
     1921 ggcgcagaag agctcgtact cccccggctc cagcgacgac aggtcgacct tggtcgactc
     1981 gcctgcaccc aggtcggggg tcttgatcgg ggtgtcgagc accgacacgt tgtgggccat
     2041 cgtcccgctg ttggtcacgt tgatcgagcc gccgagcgga accacgatcg ccgaggggtt
     2101 gatggcgaac tcggtcaggg acacctcgac gggagccgcc ggtgcggcct ccccggagcc
     2161 gttcccgtcg cccgcggtca ccgcgacgac gccgaccatc aagagggcga agatcgacgt
     2221 ggcggccgcg aagcgggcga gcatggtctg gttggcagga ctcacgacag gacctcgagc
     2281 agatcagcgg ttgcgttgcg tgtccatcgt ctgcacgcac ggccgcggcc gacaggggcc
     2341 gaggtcccag atctgatggg accaaggtcc ctgtccgttc cggctccgac ctgcgacgat
     2401 gagccgatga ccatccgggt gttcctcctc gacgaccacg aggtcgtgcg gcgcggcgtc
     2461 cgggacctga tcgagagcga ggacgatctc accgtcgtcg gcgaagcgtc cacggaggcc
     2521 gaggccctcg agcgcatcgg tctcgcccgc cccgatgtcg ccgtcctcga cgtgcagctc
     2581 gaacagggct cgggcatcga ggccgcgcgc gagatccgct cgcgccaccc cgagatccgc
     2641 tgcgtgatgc tcacctcgtt cgccgacgac gaggcactcg tcgacagcat catggccggc
     2701 gcctcggggt acatcctcaa gcaggtccgc ggccacgacc tgatcgaggg cgtccgcaag
     2761 gtaggagccg gcggttcgct gctcgacccc gtcatgacct cgcgggtcct cgaccggctc
     2821 cgcgagggga ccgaggagga cgagcgcacc gcgtcgctga ccccgcagga gcgcaggatc
     2881 ctcgacctcc tcgccgaggg tctcaccaac cgccagatcg gtgaacgcct ccacctcgcc
     2941 gagaagacgg tgaagaacta cgtgtcgaac ctcctcatga agctgggcat ggcccgccgc
     3001 accgaggccg ccgtgtacgc cgcccgccag gcggaccgcc aggctcgtgc cgcgcaccac
     3061 agcagtgctg ggacccgctg aaccggacgg tgcgctgcgt accatcgggt gggtggacga
     3121 ctcgacgcgc ccggccgacc tccagcgcct gatcgctgcc atcgcctcga tcggcgccga
     3181 cctcgacctc cccgtcgtcc tccgccggat caccgaggcg gcgaccgagc tcgtcggtgc
     3241 cacctacggg gcgctgggcg tgctcagccc cgacggcacc cacctctccg ccttcgtcac
     3301 ggtgggcatc gacgaggaga cggcggacga gatcggcgag ctgccgaagg gccacggcat
     3361 cctcggcctg ttgatcgtgc accccgagcc gctgcgcctc gacgacctcc gccagcaccc
     3421 cgagagcttc ggcttcccac cgaaccaccc gcccatgacg tcgttcctcg gggtccccat
     3481 cca
//
LOCUS       DATMFS010000226         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_78352, whole genome shotgun sequence.
ACCESSION   DATMFS010000226 DATMFS010000000
VERSION     DATMFS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78352"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..2272
                     /gene="infB"
                     /locus_tag="VNQ33_04150"
     CDS             269..2272
                     /gene="infB"
                     /locus_tag="VNQ33_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWJ97327.1"
                     /translation="MGGPRPNVPGAGVSPGGPGGRPGGPGGGPGGRPGGPAGRRPPRR
                     KGRRRRNREELQPMDLPSYTPDNSPVPDGVVVVERASTPQELGPKLNRTAADVVRFLM
                     QQGEMVTATQSLTDDMIELFVAEIGAEVRLVDPGEEQEVELQKVLGVEELDDEADEEA
                     TEWRAPVITVMGHVDHGKTKLLDRIRNANVVAGEAGGITQHIGAYQVVKDGKAITFID
                     TPGHEAFTAMRARGADATDIVILVVAADDGVMPQTIEAIDHARAADVPIVVAVNKIDR
                     DNADPDKVLAQLAERGLVPESWGGDTIVCQISALQEIGIDELLDNLLVVAELEDLRAN
                     PKGRAKGVVLEAKLDPGRGPVATILVQRGTLKVGDPLVAGPAWGRVRALINDKGEQVK
                     SAGPSTPVQVLGLSDVATAGDDFVVAKDERTAAKVAEQREHYKRIANISGSASVFHGG
                     AKLEDIFSQIQAGEAATLNLIIKADVTGSLEAVTASLKKLERDEVKLSFVREGVGGIL
                     KSDIELAMASNATIIGFNVRPDRQVREFAATEDVEIRTYEVIYHLLEDIEAAMVGMLA
                     PEFEEVVTGEAEVREIFSVPKIGKIAGCYVTNGQITRGSKVRFLRDGTIIWKGAISSL
                     RRFKDDVREVNQGFECGIGLTDFQDLKDGDIIETYEDREIPRS"
     gene            2408..2707
                     /locus_tag="VNQ33_04155"
     CDS             2408..2707
                     /locus_tag="VNQ33_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWJ97328.1"
                     /translation="MFASVLVVDLHLPMTDSLKAKRAVITPILEGARRRHRVAVSETD
                     HQDQWQRSELAFATVASSETQARAVLDAVERFVWSFPEVEVITARRSWLDEEEDD"
     gene            2707..3138
                     /locus_tag="VNQ33_04160"
     CDS             2707..3138
                     /locus_tag="VNQ33_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HWJ97329.1"
                     /translation="MPKRSTARDYPRTARLNTLVRDILARELGRIDDERLEFVTLTSV
                     VVDGDLKKAVAYYDHSRGDDADEEVAEAFDEHKSRLQRAIGREAHIKRTPDTRFELDG
                     VLRSAQHIESVIRTLPDLDPGATPVEYDPDLYKPEPSDDDA"
BASE COUNT          507 a         1199 c         1087 g          427 t
ORIGIN      
        1 gccggccccg ccccgtgcgg cgccgcccgc tgcgccgcct cgaccctcgg ctccgccggc
       61 cgccccggcc gccccggcgg cacccgcgac gccgcccgtg tccgcatccg gcaagccgat
      121 cccgccgccc ccgccacccc gttcgctcac cggcaagccg atcccgccgc cccctggcat
      181 gggcgggcgc gtctcacgtc cggctcccgg tcggcccggt ggcccgtctg gcatgccggc
      241 ccgcacgggt ggcggtccca accgccccat gggtggtccg cgccccaacg tccccggcgc
      301 cggcgtctcg cccggcggtc ccggcggccg ccccggcggt cctggtggcg gcccgggcgg
      361 tcgtcccggt ggtcccgctg gtcgccggcc cccgcgccgc aaggggcgcc gccgccgcaa
      421 ccgcgaagag ctccagccga tggacctccc gtcgtacacc ccggacaact ccccggtgcc
      481 cgacggcgtg gtcgtggtcg agcgggcctc caccccgcag gagctcggtc ccaagctgaa
      541 ccgcaccgcc gccgatgtgg tccggttcct catgcagcag ggcgagatgg tcacggccac
      601 ccagtccctc accgatgaca tgatcgagct gttcgtcgcc gagatcgggg ccgaggtccg
      661 cctcgtcgat ccgggcgagg agcaggaggt cgagctgcag aaggtgctcg gcgtcgagga
      721 gctcgacgac gaggccgacg aggaggccac cgagtggcgg gctccggtca tcaccgtcat
      781 gggccacgtc gaccacggca agaccaagct gctcgaccgc atccgcaacg ccaacgtcgt
      841 cgcgggtgag gccggtggca tcacccagca catcggtgcg taccaggtcg tcaaggacgg
      901 caaggccatc accttcatcg acaccccggg ccacgaggcg ttcaccgcca tgcgcgcccg
      961 cggcgcggat gccaccgaca tcgtcatcct cgtggtggcg gccgacgacg gcgtcatgcc
     1021 ccagaccatc gaggccatcg accacgcccg ggcggccgac gtgccgatcg tcgtggcggt
     1081 caacaagatc gaccgcgaca acgccgatcc cgacaaggtc ctggcccagc tcgccgagcg
     1141 cggcctggtg cccgagtcct ggggcggcga caccatcgtc tgccagatct cggcgctgca
     1201 ggagatcggc atcgacgagc tgctcgacaa cctcctggtc gtcgccgagc tcgaagacct
     1261 ccgggccaac ccgaagggcc gggccaaggg cgtcgtgctc gaggccaagc tcgacccggg
     1321 acgcggtccc gtggccacga tcctcgtgca gcgaggcacc ctcaaggtcg gcgacccgct
     1381 ggtcgccggt cccgcctggg gccgcgtgcg agcgctcatc aacgacaagg gcgagcaggt
     1441 gaagtcggcg ggtccgtcga cgcccgtcca ggtcctcggc ctctccgacg tcgccaccgc
     1501 cggtgacgac ttcgtggtcg ccaaagacga gcgcacggcg gccaaggtgg ccgagcagcg
     1561 cgagcactac aagcgcatcg ccaacatctc cggctcggcc tccgtgttcc acggcggcgc
     1621 caagctcgag gacatcttca gccagatcca ggcgggcgag gcggccacgc tcaacctgat
     1681 catcaaggcc gacgtgaccg gttcgctcga agcggtcacg gccagcctga agaagctcga
     1741 gcgcgacgag gtgaagctgt cgttcgtccg tgagggcgtc ggcggcatcc tcaagtccga
     1801 catcgagctg gcgatggcct ccaacgccac gatcatcggc ttcaacgtgc gccccgaccg
     1861 ccaggtccgc gagttcgcgg cgaccgagga cgtcgagatc cgcacctacg aggtgatcta
     1921 ccacctcctc gaggacatcg aggcggccat ggtgggcatg ctcgcccccg agttcgaaga
     1981 ggtggtcacc ggcgaggccg aggtccgcga gatcttctcg gtgccgaaga tcggcaagat
     2041 cgccggctgc tacgtcacca acgggcagat cacccgcggc tccaaggtcc gcttcctccg
     2101 ggacggcacc atcatctgga agggcgccat ctcgtcgctc cgccgcttca aggacgacgt
     2161 ccgcgaggtc aaccagggct tcgagtgcgg catcggcctc accgacttcc aggacctcaa
     2221 ggacggcgac atcatcgaga cctacgagga tcgcgagata cctcgttcgt gaggagacgc
     2281 ctgccctgac tcggcggtgg ggccgggtct gaggcggttt cgggtcgcct gtgcaccgca
     2341 ggggtgcatg cgtgacccga aatcggatcg gttggcggga tcgacgggtc ggtaggctcg
     2401 ttccgctgtg tttgccagtg tcctggtcgt cgacctccac ctcccgatga ccgattcgct
     2461 gaaggcgaag cgtgcggtca tcacgcccat cctcgaaggc gctcgccgcc ggcaccgggt
     2521 ggcggtgtcg gagaccgacc accaggatca gtggcagcgc agcgagctgg ccttcgccac
     2581 ggtggcgtcc agtgaaaccc aggcccgagc ggtgctcgac gccgtggagc ggttcgtgtg
     2641 gtcgttcccc gaggtcgagg tcatcaccgc ccgccggtcc tggctcgacg aggaagagga
     2701 cgactgatgc cgaagcgatc caccgcccgg gactaccccc gcaccgcgcg cctgaacacc
     2761 ctggtgcgcg acatcctcgc ccgcgagctg gggcgcatcg acgacgagcg cctggagttc
     2821 gtgaccctga cgtcggtcgt ggtcgacggc gacctcaaga aggccgtggc ctactacgac
     2881 cactcccgcg gcgacgacgc cgacgaggag gtggccgagg cgttcgacga gcacaagagc
     2941 cggctccagc gggccatcgg ccgcgaggcg cacatcaagc gcacgcccga cacccgcttc
     3001 gagctcgacg gcgtgctgcg cagcgcgcag cacatcgagt cggtcatccg caccctgccc
     3061 gacctcgacc ccggcgccac gccggtcgag tacgaccccg acctgtacaa gcccgagccg
     3121 agcgacgacg acgcctgatg gctcggcgcg ggggagggca ccaggggccc gacggcctgg
     3181 tggtggtcga caagcccacc ggctggacca gccacgacgt
//
LOCUS       DATMFS010000227         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_102340, whole genome shotgun sequence.
ACCESSION   DATMFS010000227 DATMFS010000000
VERSION     DATMFS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102340"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VNQ33_04165"
     CDS             complement(<1..946)
                     /locus_tag="VNQ33_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97330.1"
                     /translation="MTVVSDASETERAAPDPTRSAETRPATDTLRTDQVAPFRPAILG
                     IRGGTTAVSVALASPAFTEGDLWVVGWCFLVVAYNVFRIVRPVRYVDDTRSLVRVIGE
                     VAFHTIAVAATGYWESPFIFALITAVMVAGFARGFAFALRVSIASCLAIGIPFMLQDG
                     STVTDLRTTAQWTVELILVALIAGYARRVTGEADRAHSLALDRLGRLADANTLLYSLH
                     RVAQSLPASLDMEDVLDTTMGRLRDLFSFDGAAVLLLDETDESWVVVRWDGPRPAPSY
                     QTHELPGPLREALAVRHPVMVDELLHEGGGVSGEMASGL"
     gene            1001..1600
                     /gene="coaE"
                     /locus_tag="VNQ33_04170"
     CDS             1001..1600
                     /gene="coaE"
                     /locus_tag="VNQ33_04170"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228820.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWJ97331.1"
                     /translation="MLLIGLTGGIGSGKSTVSARLAEHGGVVIDADAIVHELQAPGQP
                     VLVAMAERFGPEILEADGTLRRQAVADIVFNDTDALRDLGAIVHPAVAVEIERRIAEQ
                     VDTGRVVILDVPLLVESGRDDMAATIVVDVDPEIAVARLIEHRAFSDADARARVARQA
                     SREERVARADFLIDNSGTVAELEAQADAAWAWIEGLPRR"
     gene            1649..>2716
                     /locus_tag="VNQ33_04175"
     CDS             1649..>2716
                     /locus_tag="VNQ33_04175"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HWJ97332.1"
                     /translation="MAQQVKGVVATTKGAPVSVETIEVPDPGPGEALIAVQACGVCHT
                     DLHYREGAINDDFPFLLGHEAAGIVESVGPDVTDVAPGDFVILNWRAVCGECRSCRRG
                     RPQYCFATRNATQKMTLDGRDLSPALGIGAFAEKTLVAAGQATKVDARARPEAAGLLG
                     CGVMAGIGSAMFTGEVGPGDSVAVFGCGGVGDAAIMGARLAGAAKIIAVDLDAAKLEI
                     AKELGATHTVLASEVDVVDAVQAATDGHGADVCIEAVGHPAVLEQAFYARDLAGTVVQ
                     VGVPNPEMRIELPMIDFFGRGGRLKPSWYGDCLPSRDFPMLIDLYLQGRLDLDRFVSE
                     TIALDQVEEAFHRMERGEVLRS"
BASE COUNT          392 a          952 c         1010 g          362 t
ORIGIN      
        1 agaggcccga tgccatctcc ccggacacgc cgccgccctc gtggagcagc tcgtcgacca
       61 tgaccgggtg gcggaccgcc aacgcctcgc gcagcggccc gggcagttcg tgggtctggt
      121 acgagggtgc cggccggggg ccgtcccagc ggaccaccac ccagctctcg tcggtctcgt
      181 cgaggagcaa gacggcggcg ccgtcgaacg agaacaggtc ccggagccgg cccatcgtcg
      241 tgtcgagcac gtcctccatg tcgagcgacg ccggcaggga ctgggccacg cggtgcagcg
      301 agtagaggag ggtgttggcg tcggccaggc gtcccaagcg gtcgagggcg agggagtgcg
      361 cccgatcggc ctcgccggtg acccgccggg cgtacccggc gatcagcgcg acgaggatga
      421 gctccaccgt ccactgcgcc gtggtccgca ggtcggtcac cgtggagccg tcctgcagca
      481 tgaacgggat gccgatggcg aggcaggagg cgatgctgac gcgcagcgcg aaggcgaacc
      541 cccgggcgaa cccggccacc atcaccgcgg tgatcagggc gaagatgaac ggcgactccc
      601 agtagccggt ggcggccacg gcgatggtgt ggaaggcgac ctcgccgatg acccgcacga
      661 ggctgcgggt gtcgtccacg tagcgcaccg ggcgcacgat gcggaagacg ttgtaggcga
      721 cgaccaggaa gcaccagccg accacccaca ggtcgccctc ggtgaacgcc gggctggcca
      781 gggcgaccga cacggccgtg gtgccgccgc ggatgccgag gatggccgga cggaacgggg
      841 cgacctggtc ggtgcgcagc gtgtcggtcg ctggccgggt ctcggccgag cgcgtcgggt
      901 ccggcgcagc gcgctcggtc tcagaggcgt cggacacgac cgtcatcggt ggtcaggcta
      961 ggactccggt ggcagcccgt gcgggtccta ccgtgtgccc atgctgctca tcgggttgac
     1021 gggagggatc gggtccggga agtcgaccgt gtcggcgcgc ctggccgagc acggcggcgt
     1081 ggtgatcgat gctgacgcca tcgtccacga gctgcaggcg ccgggccagc ccgtgctggt
     1141 cgcgatggcc gagcgcttcg gcccggagat cctcgaggcc gatgggacgc tgcgccgcca
     1201 ggccgtggcc gacatcgtct tcaacgacac cgacgccctc cgggacctcg gggcgatcgt
     1261 ccaccccgcg gtcgcggtgg agatcgagcg ccggatcgcc gagcaggtgg acaccggccg
     1321 cgtggtgatc ctcgacgtgc cgctgctcgt cgagtccggg cgcgacgaca tggccgcgac
     1381 gatcgtggtc gacgtcgatc ccgagatcgc cgtcgcccgg ctgatcgagc accgggcctt
     1441 ctccgacgcc gacgcccggg ctcgggtcgc ccgccaggcg tctcgggagg agcgggtcgc
     1501 cagggccgac ttcctcatcg acaacagcgg caccgtcgcc gagctggagg cccaggccga
     1561 cgcggcgtgg gcctggatcg agggccttcc gcgccgctga cccccgcggg gtacggtccg
     1621 atcgaaggtc gaaccgaagg ggtcagcgat ggcacagcag gtcaagggcg tggtcgccac
     1681 caccaaggga gcgccggtct cggtcgagac catcgaggtc ccggaccccg gacccggcga
     1741 ggcgctcatc gcggtccagg cctgcggggt gtgccacacc gacctgcact accgggaggg
     1801 cgcgatcaac gacgacttcc cgttcctgct cggccacgag gccgcgggca tcgtggagtc
     1861 ggtcggtccc gacgtcaccg acgtggcgcc gggggacttc gtgatcctca actggcgggc
     1921 ggtgtgcggc gagtgccggt cgtgtcgccg cgggcgtccg cagtactgct tcgccacccg
     1981 caacgccacc cagaagatga cgctcgacgg gcgcgacctc agcccggcgc tgggcatcgg
     2041 cgccttcgcc gagaagaccc tcgtcgccgc cggccaggcc accaaggtcg acgcccgggc
     2101 ccgtcccgaa gccgccggcc tgctgggctg cggcgtcatg gcgggcatcg gctcggccat
     2161 gttcaccggc gaggtcggac cgggcgactc cgtcgccgtc ttcggctgcg gcggtgtggg
     2221 cgatgccgcc atcatgggcg cccgcctcgc cggcgcggcg aagatcatcg ccgtcgacct
     2281 cgatgcggcc aagctcgaga tcgccaagga gctcggtgcc acccacaccg tgctggcctc
     2341 ggaggtcgat gtcgtcgacg ccgtccaggc ggccaccgac ggccacggtg ccgacgtgtg
     2401 catcgaggcg gtcggccacc cggccgtact ggagcaggcg ttctacgccc gagacctggc
     2461 cggcaccgtg gtccaagtcg gggtgccgaa cccggagatg cgcatcgagc tgccgatgat
     2521 cgacttcttc ggccggggcg gccgcctcaa gccgtcgtgg tacggcgact gcctcccgag
     2581 ccgggacttc ccgatgctca tcgacctcta cctgcagggg cgcctcgatc tggaccggtt
     2641 cgtgtccgag acgatcgccc tcgaccaggt cgaggaggcg ttccaccgca tggagcgcgg
     2701 cgaggtgctc cggtcc
//
LOCUS       DATMFS010000228         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_288239, whole genome shotgun sequence.
ACCESSION   DATMFS010000228 DATMFS010000000
VERSION     DATMFS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288239"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="fbiC"
                     /locus_tag="VNQ33_04180"
     CDS             complement(<1..322)
                     /gene="fbiC"
                     /locus_tag="VNQ33_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase"
                     /protein_id="HWJ97333.1"
                     /translation="MSLTTGSSWDQPLPELMEQARAVRGARTGTRITYSPKVFIPLTM
                     LCRDKCGYCTFAQPPARLDAPFLTPEQVLRIARQGASAGCHEALFTLGERPEERYPVA
                     AEWLA"
     gene            445..1791
                     /locus_tag="VNQ33_04185"
     CDS             445..1791
                     /locus_tag="VNQ33_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97334.1"
                     /translation="MSPRTTTDAPRKVAAALAVALLLGAVAVVADGASAAASTPVMGR
                     NRVSAADLAGWFRSKGKTSKATVSIDVLAAHFISEGADEGVAGDLAFAQSIVETGYFA
                     FSARVLPSYNNFSGLGAVDGGTGAAAFSTAQLGVRAQIQHLRAYADPAVTTAKLAHPV
                     IDPRFSLVAPKGKAPTWEQFGNGIWATDPGYAAKVLNIYGQILAYAGNPSSPTTTTTA
                     PTTTTTAPPVRAFPPFASASAAVDQAYGDILARTPSATERSAAAAALDAGAKTPSQLM
                     AELVAGEGAVDAQPVARLYLAGLGRLPDRSGLQYWTRRHAAGIALVRLAEQFIASSEF
                     QRRYGNPTNAGYVDVLYRNVLDRGADASGSDYWNRRLAFGVITRAGLLVQFSESNENR
                     TKTATKVEASLVYVGMVLRAPDPSVLTWWATKKAGGYPLSTLTDLVYGSAAYRARF"
     gene            complement(1813..>2381)
                     /gene="pgl"
                     /locus_tag="VNQ33_04190"
     CDS             complement(1813..>2381)
                     /gene="pgl"
                     /locus_tag="VNQ33_04190"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWJ97335.1"
                     /translation="LSGGETARGCYEHLADVSGSQIDWWKVDVYWGDERCVPHDDPAS
                     NYYLAREALLDRVGAANATHLMRCAEGPDPYQLRLGDLGRIDLIHLGMGADGHTASLF
                     PESQALDADPGRLVAMNDDPLGNNPYQRMTLTFAGIARAALAIVTVTGEEKAPALAAI
                     ANGASLPAARIASEQVVWLVDEAAASLL"
BASE COUNT          323 a          879 c          852 g          327 t
ORIGIN      
        1 cggcgagcca ctcggcggcc acgggatagc gctcctcggg ccgctcaccc agggtgaaca
       61 gcgcctcgtg gcagccggcc gacgcacctt ggcgggcgat gcgcagcacc tgctccgggg
      121 tgaggaaggg cgcatcgagc cgggccggcg gctgcgcgaa ggtgcagtag ccgcacttgt
      181 cgcgacacag catggtgagc gggatgaaca ccttggggga gtaggtgatg cgcgtgccgg
      241 tgcgggcgcc gcgcaccgcg cgggcctgct ccatgagctc gggcagcggc tggtcccagg
      301 aggaaccggt ggtcagcgac atgggcggca ggctacctgc gggacctggt cgtgcggccc
      361 gcccggccca cgagcggcgg cctcacccaa cgtgatgaga acctcactcc ttgaggttct
      421 gctggtcccg ccgatggagt tcgcatgtca ccacgcacca cgacggacgc gccgcgcaag
      481 gtggccgccg ctctggctgt cgccttgctc ctcggtgccg tggcggtcgt cgccgacggc
      541 gcctcggcgg ccgcctccac accggtcatg ggccgcaacc gggtgagcgc cgccgacctg
      601 gcggggtggt tccgctccaa gggcaagacc tcgaaggcga ccgtgtcgat cgacgtgctg
      661 gccgcccact tcatctccga gggtgccgac gagggcgtgg ccggcgacct ggcgttcgcc
      721 cagtcgatcg tcgagaccgg ctacttcgcc ttctccgccc gggtgctgcc ctcgtacaac
      781 aacttctcgg gcctcggggc cgtcgacggc gggaccggcg cggcggcctt ctcgacggcg
      841 cagctcggcg tccgggccca gatccagcac ctgcgcgcct acgccgaccc ggcggtcacg
      901 acggcgaagc tcgcccaccc cgtgatcgac ccccgcttct cgctggtggc gccgaagggc
      961 aaggccccca cctgggagca gttcggcaac ggcatctggg ccaccgaccc cggctacgcc
     1021 gccaaggtcc tcaacatcta cggccagatc ctggcctacg ccggcaaccc atcgtcgccg
     1081 acgaccacga ccaccgcacc caccaccacg accacggcgc cgccggtgag ggccttcccg
     1141 cccttcgcgt cggccagcgc ggcggtcgac caggcgtacg gcgacatctt ggcccgcacg
     1201 ccctccgcca ccgagcgctc ggccgccgcg gcggccctcg acgccggcgc caagacgccc
     1261 tcccagctga tggccgagct ggtggccggt gagggggccg tcgacgccca gccggtcgcc
     1321 cgcctctacc tggccggcct cggccggctc ccggaccgca gcggcctcca gtactggacc
     1381 cggcgccatg cggccggcat cgcgctcgtg aggctggcgg agcagttcat cgcgtcgtcg
     1441 gagttccagc gccgctacgg caacccgacc aacgccgggt acgtcgacgt gctgtaccgc
     1501 aacgtgctcg accgcggcgc cgatgccagc ggctcggact actggaaccg ccgcctcgcc
     1561 ttcggcgtga tcacccgggc gggcctgctc gtgcagttca gcgagtccaa cgagaaccgg
     1621 accaagacgg cgaccaaggt cgaggcctcc ctcgtctacg tgggcatggt gctgcgggcg
     1681 cccgatccgt cggtcctcac ctggtgggcg acgaagaagg cgggtgggta cccgctcagc
     1741 acgctcacgg acctcgtcta cggcagcgcc gcctaccggg cgcggttctg acggctctgg
     1801 cggcccggcc gctcagagga gcgaggcggc ggcctcgtcg acgagccaga cgacctgctc
     1861 gctcgcgatc ctcgccgccg gcaggctcgc tccgttcgcg atcgcggcca gcgccggcgc
     1921 cttctcctca ccggtgaccg tcacgatcgc gagcgcggcg cgggcgatgc cggcgaaggt
     1981 cagcgtcatc cgctggtagg ggttgttgcc cagcgggtcg tcgttcatgg ccacgagacg
     2041 gccagggtcg gcgtcgagcg cctgggactc ggggaacagc gaggcggtgt ggccatcggc
     2101 gcccatgccg aggtggatga ggtcgatgcg gcccaggtcg ccgaggcgca gctggtacgg
     2161 gtccggtccc tcggcgcagc gcatcaggtg ggtggcgttg gcggcgccga cccggtcgag
     2221 gagggcttcg cgggccaggt agtagttcga cgccgggtcg tcgtggggga cgcaccgctc
     2281 gtcgccccag tacacgtcga ccttccacca gtcgatctgg ctgccggaga cgtcagccag
     2341 gtgctcgtag cagccgcggg ccgtctcgcc accggagagg g
//
LOCUS       DATMFS010000229         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_288261, whole genome shotgun sequence.
ACCESSION   DATMFS010000229 DATMFS010000000
VERSION     DATMFS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288261"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VNQ33_04195"
     CDS             <1..475
                     /locus_tag="VNQ33_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97336.1"
                     /translation="TYVRSRTLDSIAKPGEGEPPPFVSRIPHEFPPPVPPGAHALAAA
                     DAAYSMAPYLLGPDDALVITAAWPPCRCASISLWNRQLQTFDYLRGPVSLNRAQAVAD
                     ADGSVRVVVAHRDPGVSNWLTTEGRPFGLMFWRFMLPEGPIATPIAEVVPVDSLR"
     gene            935..>2768
                     /locus_tag="VNQ33_04200"
     CDS             935..>2768
                     /locus_tag="VNQ33_04200"
                     /inference="COORDINATES: protein motif:HMM:NF023835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HWJ97337.1"
                     /translation="MSDTTEALEAAAWAAADGSATPEQLTLLEADPERWRDLVEDLLE
                     DLDDRLDAVRQLTGPERNQVVADFEAELAQLEAAYDLLSKTDDPVAAIAAADPAGEVR
                     LQASWASGQIVVWAAGPGTEPATAEELSDRLEAIGGPAVGWSVHPAVVLPGGSRAAAL
                     SIPIGEALGWLVAVGGGLGREGVGASVAWLGRVAVAAVRLVARGAIVPTLPGAKQPDG
                     RAIELGVKWVPALVEEATVSAMAAAMPGPVTVLAHADPRNVTLAVQGAVVDTIVRQAA
                     GMLEFPAPPPVVRTTAAVAEAFVTRLDGTSFEAPVAAGAEVSKRIDRWAKPLTGTARV
                     KLVITLDPPDSGNAWFLSVLGPGAEGTLLPIEQALADGKATKPLADELNRVERAFPAL
                     LRPGALRRGQVYLSQDEAWELMTNTGPLLEAAGFEVRVPALSRKKPSPSLRLFAESMG
                     GESVVGAHQLANVSWSVLFDDVELTAEDIARLAAEARPLVRSHGKWVELDAVDLKEAA
                     AALAERAEKTKMTGAEILRHSVGLEGNHLAGGVTVDGSGWASELFRKASHISTDPVTT
                     PEGFVGELRGYQAEALAWLGFLHTVDLGGCLALDMGLGKTPTMLA"
BASE COUNT          382 a         1013 c         1009 g          364 t
ORIGIN      
        1 cacctacgtc cgcagccgga cgctcgacag catcgccaag ccgggggagg gggagcctcc
       61 gccgttcgtg tcccgcatcc cccacgagtt cccgccgccg gtcccgcccg gcgcccatgc
      121 cctggcggcg gccgacgcgg cgtacagcat ggcgccgtac ctgctcggac ccgatgacgc
      181 gctggtgatc acggccgctt ggcccccgtg ccgctgcgcc agcatctcgc tctggaaccg
      241 tcagctccag acgttcgact acctgcgagg tccggtgagc ctcaaccggg cgcaggcggt
      301 ggctgacgcc gacggcagcg tccgggtggt ggtcgcccac cgcgaccccg gcgtgtcgaa
      361 ctggctcacc accgaaggcc ggcccttcgg cttgatgttc tggcgcttca tgctccccga
      421 gggccccatc gccacgccca tcgccgaggt cgtccccgtc gactcgctgc gctgatcggg
      481 cggctcggga ggggacgcgg gaaggagagg ggggctcgag gcgagcgcca gagcgaagcg
      541 caacagaccg gggagcaacg tggaagatga atgccgctaa ccgaccacga tcctccaagt
      601 tccgccacag gactccaact tggaggataa gggcggtgag cgtccacgat cctccaagtt
      661 ggagtcccgt ggcgagcgat tgaggagacc gggcccgagg gcgtggcgac cgggggcgga
      721 cgggggtggc gaccgagggc gtggcgacgg ggggcagacg ggatgcacga ggaccgcgtc
      781 ggcaggggta cactggttct tgctcggcgt ccctcctgcg caggcggggg gtggagctgg
      841 tcccgtccga ggccagccca cacgaacgcc ctgagcggac ggtccgacga tcgggtgatc
      901 ggcgggctac ggcgcaggga gcgccggacc agagatgtct gacaccaccg aagcgttgga
      961 agcggccgcg tgggcggcag cagacggcag cgcgacaccc gaacaactga cgctgctcga
     1021 ggccgacccc gagcggtggc gcgacctcgt cgaggacctc ctcgaggatc tcgacgaccg
     1081 cctggacgcg gtgcgccagc tgaccgggcc cgagcgcaac caggtcgtcg ccgacttcga
     1141 agcggagctc gcccagctcg aagccgccta cgacctgctc tccaagaccg acgaccccgt
     1201 ggccgccatc gccgccgccg acccagcggg cgaggtgcgg ctgcaggcat cgtgggcgtc
     1261 ggggcagatc gtcgtgtggg cggctggtcc cggcaccgag ccggcgacgg ccgaggagct
     1321 gtcggaccgg ctggaggcca tcggcggccc ggcggtcggc tggagcgtcc acccggcggt
     1381 ggtcctgccg ggcggctcgc gggccgcggc gctgtcgatc cccatcggtg aggccctggg
     1441 ctggctcgtc gccgtgggcg gcggactggg tcgcgagggc gtcggcgcga gcgtcgcctg
     1501 gctcggacgg gtcgccgtcg ccgcggtgcg gctggtggcc cggggcgcga tcgtcccgac
     1561 gctcccaggc gccaagcaac ccgatggccg cgccatcgag ctcggcgtca agtgggttcc
     1621 ggccctcgtc gaggaggcca ccgtctcggc catggccgcg gccatgcccg gtccggtgac
     1681 cgtgctcgcc cacgccgatc cgcgcaacgt gaccctcgcc gtccagggtg cggtcgtcga
     1741 cacgatcgtc cgccaggccg ccggcatgct cgagttcccg gctccccctc ccgtggtccg
     1801 caccaccgcc gcggtggccg aggccttcgt gacccgcctc gacggcacct ccttcgaggc
     1861 tccggtggcc gctggtgccg aggtctccaa gcgcatcgac cgctgggcga agcccctcac
     1921 gggcaccgcc cgggtcaagc tcgtcatcac gctcgacccg ccggactcgg gcaacgcctg
     1981 gttcttgtcg gtgctcggcc ccggggccga gggcacgctg ctgcccatcg agcaggcgct
     2041 ggccgacggc aaggccacca agcccctggc cgacgagctc aaccgcgtcg agcgggcgtt
     2101 ccccgcgctg ctccggcccg gcgccttgcg ccgcggccag gtgtacctga gccaggacga
     2161 ggcctgggag ctgatgacca acaccggccc gctgctggaa gcagccgggt tcgaggtccg
     2221 ggtgccggcc ctgtcccgca agaagccgtc gccgtcgctg cgcctcttcg ccgagtcgat
     2281 gggcggcgag tccgtcgtcg gcgcccacca gctggccaac gtgagctggt cggtgctgtt
     2341 cgacgacgtc gagctgacgg ccgaggacat cgcccgtctc gcggccgagg cacggccact
     2401 cgtccgctcg cacggcaagt gggtcgagct cgatgccgtc gacctgaagg aagcagcggc
     2461 cgccctggcc gaacgggccg agaagacgaa gatgaccggg gcggagatcc tccgccactc
     2521 ggtgggcctc gagggcaacc acctcgccgg tggcgtcacc gtcgacggca gcggctgggc
     2581 ctccgagctc ttccgcaagg cgtcgcacat ctccaccgat cccgtcacga cgcccgaggg
     2641 cttcgtcggc gagctgcgcg gctaccaggc cgaggcgctc gcctggctcg ggttcctcca
     2701 caccgtggac ctcggcggct gcctcgccct ggacatgggc ctgggcaaga cgcccacgat
     2761 gctcgccc
//
LOCUS       DATMFS010000230         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_195105, whole genome shotgun sequence.
ACCESSION   DATMFS010000230 DATMFS010000000
VERSION     DATMFS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195105"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1968
                     /locus_tag="VNQ33_04205"
     CDS             439..1968
                     /locus_tag="VNQ33_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWJ97338.1"
                     /translation="MDALGIDIGSTNVKVVLVRDDGTTLTAAHRPLTWFRDGPTAEQD
                     GGAMWAAVRGALADAVAAVGPETVAAVGGIGLCGQYSSIVPVDAAGTPLAPMRLYLDQ
                     RGTPHCLDILGRHEEAFLTWLDRHPIPPVGGGLALGHLLAFQHDEPAVHERTAAYLEP
                     VDYVTARLTGTIAATQGSMFASQLIDNRTLGATAYDAELVALAGIDPSRLPPLVEVGS
                     VVGTVLPEVADGLGLPTGVPVITGMTDSCAGAHATGAEARGRVGIAIGTTGVVLSSVE
                     ALGVDLDHEVLAMPGLRTDRYLVSAENGIAGRAVEHVLAHLVHATDALGDHGAADPFA
                     GFDAALDASPAGARGVRFLPWLSGSLSPQADAAMRGGFVGVSLDSQRVDLVRAAAEGV
                     AHNLRWLLDPVEAFTGQRADEVVLTGGAARSAAWSQVLADVLQRPVHVLQEPGHSGAR
                     ASAGWALEQLGHGGGEWVRLDRTFEPDPSTADVHAHAQGQFTSVFEALRPLGLGPVAP
                     S"
     gene            complement(2118..>3674)
                     /locus_tag="VNQ33_04210"
     CDS             complement(2118..>3674)
                     /locus_tag="VNQ33_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWJ97339.1"
                     /translation="MNGANALIRTLVDSGVDVCFMNPGTSEMHFVAALDDVREMRGVL
                     ALFEGVATGAADGYARMADKPAAVLLHLGPGLGNGLANLHNARKGHTPVVNIVGDHAT
                     YHKQYDAQLESDIETVARNVSTWLRTSASVEEVAADAADAVAVASGPPAQIATLILPA
                     DVSWSEGGTPAAPVAPAQPTPVDADAVAAIAAVLQGDEPAALFVGGRACRADALVDAS
                     RVANTTGTKLLAETFPARIERGAGRVPVDRLAYLAEFAAMQLDGLKHLVLVDSKAPVS
                     FFAYPGKASDLVPEGCTVHVLADAAGDPAGALAALAAAVGAAPDAATIQVEARPELPT
                     GPLTADGVCQALGALLPEGAIVSDEGNTSGLFAAGHTAGAPAHDWLTLTGGAIGQGLP
                     AAVGAAVACPDRKVIVLESDGSAMYTMQSWWTMAREELDVVTVLFNNASYAVLNMELN
                     RVGATEGGPKAKEMLDLTRPTMDFMSMAQGCGVPATRATTAEEFTEQFQAALAAKGPR
                     VIECIVPSII"
BASE COUNT          445 a         1303 c         1432 g          494 t
ORIGIN      
        1 acgtcgaggc cggctccggc gacgagggcg gcgatgagga gcacgaccgg tgccgggtcc
       61 gcctcccgcg gcggtcgagg gggctgatcg gggacccacc gatccgcgga ggcggtaccg
      121 ggcggaggtg gcgggagcgg cggaggcggc gcccacccga cagcggtcgc cccgtgcacc
      181 gggctgggta ccggcccggg cggtggcggg agcggccggc cctggggccg cgcccagcct
      241 tcgtccggtc ccgtcgtcgc tggcatctga ccccctgtac cgatgcccct gccccgtgca
      301 ggtggctcca ccggcgcccg ggacggtacg tggtcccctg tgtgacggtc ccggaggggg
      361 tgtggagatc gagtgcaggc gggctccagc acgggccgga tcggggcgta gggtccgagc
      421 gccgaaaggg gaggtgccgt ggacgccctg gggatcgaca tcggttcgac caacgtgaag
      481 gtggtgctgg tccgcgacga cgggaccacg ctcaccgccg cgcaccgtcc gctcacctgg
      541 ttccgcgacg ggcccaccgc cgaacaagac ggtggcgcga tgtgggcggc cgtgcgcggt
      601 gcgctggctg atgccgtggc cgccgtcggg cctgagaccg tggccgccgt gggtgggatc
      661 gggctgtgcg ggcagtactc gtccatcgtc ccggtcgacg ccgccgggac gccgctcgcc
      721 cccatgcggc tctacctcga ccagcgcggg acgccgcact gcctcgacat cctcggccgc
      781 cacgaggagg ccttcctgac gtggctcgac cgccatccga tcccgcccgt cggcggcggt
      841 ctggccctcg gccacctgct ggcgttccag cacgatgagc ccgcggtcca cgagcgcacg
      901 gcggcctacc tggaaccggt cgactacgtc accgcccggc tcaccggcac gatcgccgcc
      961 acgcagggct cgatgttcgc cagccagctg atcgacaacc ggaccctcgg cgcgaccgcc
     1021 tacgacgccg agctcgtcgc cctggccggg atcgacccga gtcggctgcc tccgctggtc
     1081 gaggtgggca gcgtggtcgg gacggtcctg ccggaggtgg ccgatggcct cggcctcccg
     1141 accggcgtgc cggtgatcac cggcatgacc gacagctgcg ccggcgccca cgccacgggg
     1201 gccgaggcgc ggggccgcgt cggcatcgcc atcggcacca ccggcgtggt gctcagctcc
     1261 gtcgaggcgc tgggcgtcga cctcgatcac gaggtgctcg ccatgccggg gcttcggacg
     1321 gaccgctacc tcgtgtcggc cgagaacggc atcgccggac gcgccgtcga gcacgtgctc
     1381 gcccacctgg tccacgccac cgatgccctc ggcgaccacg gagcggctga cccgttcgcc
     1441 gggttcgacg ccgcgctgga cgcgtcgccc gcgggagcgc ggggcgtgcg cttcctcccg
     1501 tggttgtcgg ggtcgttgtc accgcaggcc gatgcggcga tgcgcggtgg gttcgtcggt
     1561 gtgtcgctcg acagccagcg cgtcgacctg gtgcgggcgg cggcagaggg cgtcgcccac
     1621 aacctccgct ggctgctcga cccggtcgag gcgttcaccg gccagcgcgc cgacgaggtc
     1681 gtcctcaccg gcggtgccgc tcgctcagcg gcctggtccc aggtgctggc cgacgtcctc
     1741 cagcggcccg tgcacgtgct gcaggagccc ggccactccg gcgcccgcgc ctcggcgggc
     1801 tgggcgctgg agcagttggg ccacggcggg ggcgagtggg tccgcctcga ccgcaccttc
     1861 gagcccgacc ccagcacggc ggacgtgcac gcccacgccc aggggcagtt caccagtgtg
     1921 ttcgaggccc tccgtcccct cggcctcggt cccgtcgcgc cgagctgacc catccccggg
     1981 ggggggtagc gggtgccatc gctcctgcca tggctgaact tggtgtgcgt atcgaggcag
     2041 gacagcagga accacaccaa gttcggttgg gcctcgccgt gcagagcgag acggtcagga
     2101 agccgccgcc gcgagggtca gatgatcgac gggacgatgc actcgatgac gcgggggccc
     2161 ttggcggcga gggcggcctg gaactgctcg gtgaactcct cggccgtggt ggcccgggtg
     2221 gcggggacgc cgcagccctg ggccatcgac atgaagtcca tcgtgggccg ggtgaggtcg
     2281 agcatctcct tggccttggg cccgccctcg gtggcaccga cccggttcag ctccatgttg
     2341 agcaccgcgt agctggcgtt gttgaacagc acggtgacga cgtcgagctc ctcgcgcgcc
     2401 atcgtccacc agctctgcat cgtgtacatc gccgagccgt cggactcgag cacgatcacc
     2461 ttgcggtccg ggcaggcgac ggccgcgccg acggcggccg ggagcccctg gccgatggcg
     2521 ccgccggtga gcgtcagcca gtcgtgcgcc ggcgcgcccg ccgtgtggcc ggcggcgaag
     2581 aggcccgacg tgttgccctc gtcggagacg atggcgccct cgggcaagag cgcgcccagc
     2641 gcctggcaca cgccgtcggc ggtgagcgga ccggtgggca gctcggggcg ggcctcgacc
     2701 tggatcgtgg ccgcgtcggg cgccgcgccc accgcggcgg ccagcgcggc gagggcgccc
     2761 gctgggtcgc cggccgcgtc ggcgaggacg tgcaccgtgc agccctcggg caccaggtcg
     2821 ctggccttgc cggggtaggc gaagaacgag accggcgcct tggagtcgac gaggacgagg
     2881 tgcttcaggc cgtcgagctg catggcggcg aactcggcca ggtacgcgag gcggtcgacg
     2941 gggacgcgcc cggcgccgcg ctcgatgcgg gcggggaacg tctcggccag gagcttggtg
     3001 ccggtggtgt tggcgacccg gctggcgtcg accagggcgt cggcccggca ggcccggccg
     3061 cccacgaaca gggcggccgg ctcgtcgccc tggagcacgg cggcgatggc ggcgacggcg
     3121 tcggcgtcca ccggcgtggg ctgggccggg gcgacgggag cggcgggcgt gccgccctcg
     3181 ctccagctga cgtcggcagg gaggatcagc gtggcgatct gggccggcgg gcccgaggcg
     3241 acggccacgg cgtcggcggc gtcggccgcc acctcctcga ccgaggccga ggtgcgcagc
     3301 cacgtcgaca cgttgcgggc cacggtctcg atgtcggact ccagctgggc gtcgtactgc
     3361 ttgtggtacg tcgcgtggtc gccgacgatg ttcaccacgg gcgtgtggcc cttgcgggcg
     3421 ttgtgcaggt tggccaggcc gttgccgagg ccggggccga ggtgcagcag gacggcggcg
     3481 ggcttgtcgg ccatgcgggc gtagccgtca gcggcgccgg tggccacgcc ttcgaacaag
     3541 gcgagcacgc cgcgcatctc gcgcacgtcg tcgagggcgg cgacgaagtg catctccgac
     3601 gtgccggggt tcatgaagca gacgtcgacc cccgagtcga cgagcgtgcg gatgagggcg
     3661 ttggcgccgt tcat
//
LOCUS       DATMFS010000231         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_311168, whole genome shotgun sequence.
ACCESSION   DATMFS010000231 DATMFS010000000
VERSION     DATMFS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311168"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..897
                     /locus_tag="VNQ33_04215"
     CDS             67..897
                     /locus_tag="VNQ33_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWJ97340.1"
                     /translation="MAANEFEQQAKRWRVAPGSRVHLDRIDTRSKAGAPGSKEVTTGV
                     VEGLVAQLGEWQTRLWAEGSRSVLMVLQAMDAGGKDGTIRKVFTGVNPQGVRVASFKA
                     PTPEELAHDFLWRIHKQAPAHGELGVFNRSHYEDVLIVRVDELVSKAVWKARYEQIRA
                     FEETLAAAGTTVVKFFLHISPDEQAERLQARLDDPTKRWKFAAADLDVRKDWDAYQAA
                     YEDAISETSTKGAPWYVIPADRKWYRNWAILQVLLATMTGLDPQFPPEEPGLEGLKVV
                     "
     gene            complement(907..1902)
                     /locus_tag="VNQ33_04220"
     CDS             complement(907..1902)
                     /locus_tag="VNQ33_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ97341.1"
                     /translation="MNPLQRIRSIKVKLSVVIVAAVVVTLAVNEIGLALNFKAGFRAG
                     VAAIIALGMVQLMARGMTSPLRSMERAATAMAEGDLTKRVSASSSDEVGRLAKAFNHM
                     ADELAAEDQHRRELIANVSHELRTPISALRATLENVVDGVVAPEPALLRTMLAQTERL
                     QRLVTQLLDLSRLESGGTPLHKLPFPVVDLLDAVADEAALHSPDLAFDVQIDPLDLVV
                     DGDPERLHQVIANLVENAARFAPDDTAVTLAARRAGPHVVLEVADHGPGIPPESLARV
                     FERFYRTDEARSADDGGSGLGLAIARWIVELHGGTIRAETVEPHGCRMVVRLPAR"
     gene            complement(1905..>2390)
                     /locus_tag="VNQ33_04225"
     CDS             complement(1905..>2390)
                     /locus_tag="VNQ33_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ97342.1"
                     /translation="DDYLTKPFSMRELVARVQALLRRLDALSGRTQPKALHLDDGIEI
                     DLDARRVRQHGEVVHLTPTEFDLLVGLAERPGRVLTRERLLIDVWGYRDGSGARTVDS
                     HVRALRRKLGDGVVRTVHGVGYALGESAEPAGDSDDAGAGAPAAGSSTASDGAEAAGT
                     P"
BASE COUNT          347 a          831 c          896 g          316 t
ORIGIN      
        1 tacgccgggt gcgcgacccg gccgtctcga cgcctcgccg ccggctcggg cgtagcttgt
       61 cggtccatgg ctgcgaacga gttcgagcaa caggcgaagc gctggcgcgt ggcgccgggg
      121 tccagggtgc acctcgaccg gatcgacacc cgctcgaagg cgggagcccc gggcagcaag
      181 gaggtcacga ccggggtggt cgagggcctc gtcgcccagt tgggcgagtg gcagacgcgg
      241 ctgtgggccg agggcagccg ctcggtcctg atggtcctcc aggcgatgga cgcaggcggc
      301 aaggacggca cgatccgcaa ggtgttcacc ggggtcaacc cgcagggcgt gcgggtcgcc
      361 tcgttcaagg cgccgacgcc cgaggagctg gcgcacgact tcctctggcg catccacaag
      421 caggcgccgg cccacggcga gctcggcgtc ttcaaccgct cgcactacga ggacgtgctc
      481 atcgtgcggg tcgacgagct cgtctcgaag gcggtctgga aggcccgcta cgagcagatc
      541 cgcgcgttcg aggagacgct tgccgccgcc gggacgacgg tcgtgaagtt cttcctccac
      601 atctcgcccg acgagcaggc cgagcggctg caggcccgcc tcgacgaccc gacgaagcgc
      661 tggaagttcg cggccgccga cctcgacgtc cgcaaggact gggatgcgta ccaggcggcc
      721 tacgaggacg cgatcagcga gaccagcacg aagggcgcgc cctggtacgt gatccctgcc
      781 gaccggaagt ggtaccggaa ctgggccatc ctccaggtcc tcttggccac catgaccggt
      841 ctcgacccgc agttcccgcc cgaggagccc ggcctcgagg gcctgaaggt ggtctgaccc
      901 tcggcatcat cgggcaggaa gccgcaccac catgcggcag ccgtggggct cgacggtctc
      961 ggcgcggatg gtgccgccgt gcagctccac gatccagcgg gcgatggcca gccccagccc
     1021 cgagccgccg tcgtcggcgc tgcgggcctc gtcggtccgg tagaagcgct cgaacacccg
     1081 ggcgagcgac tcgggcggga tgcccggccc gtggtcggcc acctcgagca ccacgtgcgg
     1141 gccggcccgg cgggcggcga gggtcaccgc ggtgtcgtcc ggggcgaagc gggccgcgtt
     1201 ctcgaccaga ttggcgatga cctggtgcag ccgctccggg tccccgtcca ccacgaggtc
     1261 gagcggatcg atctgcacgt cgaacgcgag gtcgggggag tggagcgcgg cctcgtcggc
     1321 cacggcgtcg aggaggtcga ccaccgggaa cggcagcttg tgcagcggcg tcccgccgga
     1381 ctcgagccgg ctcaggtcca gcagctgggt gacgaggcgc tggaggcgct cggtctgggc
     1441 gagcatcgtg cgcagcagcg ccggctcggg agccacgacc ccgtcgacga cgttctcgag
     1501 cgtcgcccgg agggccgaga tcggcgtccg cagctcgtgc gacacgttgg cgatgagctc
     1561 gcgccggtgc tggtcctcgg cggccagctc gtcggccatg tggttgaacg ccttggccag
     1621 gcgaccgacc tcgtcggacg agctggccga gacccgcttc gtgaggtcgc cctcggccat
     1681 cgccgtggcc gcccgttcca tcgaccgcag gggcgaggtc atgccccgcg ccatgagctg
     1741 caccatcccg agggcgatga tcgcggcgac gccggcacgg aagccggcct tgaagttgag
     1801 ggccaggccg atctcgttga cggcgagcgt gaccaccacc gcggcgacga tgacgacgct
     1861 gagcttgacc ttgatcgacc ggatccgctg gagcgggttc acgatcacgg ggtgccagcg
     1921 gcctcggcgc cgtcggatgc ggtggaggac ccggccgccg gggcacccgc tcccgcgtcg
     1981 tccgagtcgc cagccggctc agccgactcg ccgagcgcgt agcccacacc gtgcaccgtg
     2041 cggacgacgc cgtcgcccag cttccggcgc agggcccgga cgtgggagtc gacggtgcgg
     2101 gcaccggagc cgtcgcggta gccccacacg tcgatgagca ggcgctcccg ggtgagcacg
     2161 cgcccgggcc gctcggccag ccccacgagc aggtcgaact cggtgggggt gaggtgcacg
     2221 acctcgccgt gctggcgcac gcgccgggca tcgaggtcga tctcgatgcc gtcgtcgagg
     2281 tgcagggcct tgggctgggt gcgtccggac agcgcgtcga ggcggcgcag gagggcctgg
     2341 acacgggcca ccagctcgcg catcgagaac ggcttggtca ggtagtcgtc
//
LOCUS       DATMFS010000232         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_169801, whole genome shotgun sequence.
ACCESSION   DATMFS010000232 DATMFS010000000
VERSION     DATMFS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169801"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1214
                     /locus_tag="VNQ33_04230"
     CDS             78..1214
                     /locus_tag="VNQ33_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ97343.1"
                     /translation="MSNETPRGAGRYEVPIGIEIAAAWAWRIVVIGAALYVLALGLGH
                     FSEVTIPIAVAILLAALTIGGVDWLVRHRVPRLVASLAVVLGLVLALVGMLGLVGQQL
                     STQFDDLRDSVVEGIAQLQDWARTGPLGLSDRQLADWVDRVQSAISSSDSSVVNQATE
                     VGTQIGHFVTGFFIALFALFFFLYEGGRIWSWVVVLFPKGARPRVASSGRAAWGSLTA
                     FVRATVLVALVDAIGIAGVALILQVPLAAAIGVLVFLGAFVPIVGALVSGMVAVLVAL
                     VAQGPVTALLMLAGVIAVQQLESHVLQPFLMGAIVALHPLAILLAIATGLVVAGVVGA
                     LLSVPFAACLNSVVRHLANGEPPDTDGVLGEESRDLGDPPPATA"
     gene            1224..2414
                     /gene="ilvA"
                     /locus_tag="VNQ33_04235"
     CDS             1224..2414
                     /gene="ilvA"
                     /locus_tag="VNQ33_04235"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWJ97344.1"
                     /translation="MDLDDARAARELLDGVSEVTPLTHSRWLHALTGRDIRFKCENLQ
                     RAGSFKIRGAYTRIARLSDEERSRGVVAASAGNHAQGVALAAAQLGADATIFMPHGAA
                     LPKVAATRGYGADIRFHGSGVTEALVAASEFAEETGATLIHPFDHPDIVAGQATVGLE
                     IIEQLPEVATILVPLGGGGLAAGIGLVKTERPDITVIGVQAEAAAAYPDSLAEGMPVE
                     ADLGATMADGIAVARPGEVPFGLIRDHVDEVVTVSEESMSQALIGLLERAKLLVEPAG
                     AVGVAALLEHPDRFDGPVVPVLSGGNIDALLLLEVIRHGLASGGRFLMFRVRISDRPG
                     ELMRLLTALAEMDVNILNVHHDRGSEALGVREVEVALQVATRGPEHRADVWRRLVELG
                     YDPI"
BASE COUNT          279 a          923 c          842 g          387 t
ORIGIN      
        1 cgaatccgaa tcgcatggga cctcctgtgt cgtcgtcaac accgtgcggt gtcggcatgt
       61 tcccgtagcc tcgatgcgtg agcaacgaga cgccgcgcgg tgccgggcgg tacgaggttc
      121 ccatcggcat cgagatcgcc gcagcctggg cctggcgcat cgtcgtcatc ggcgccgcgc
      181 tgtacgtcct ggccctgggc ctgggccact tctccgaggt caccatcccc atcgccgtgg
      241 cgatcctgct ggcggcgctg acgatcggtg gcgtcgactg gctcgtgcgg caccgggtgc
      301 cgcggctcgt ggcctcgctc gcggtcgtgc tggggctggt gctcgcgctc gtgggcatgc
      361 tcggtctggt cggccagcag ctgtccaccc agttcgacga cctgcgcgac agcgtggtcg
      421 agggcatcgc ccagctgcag gactgggccc gcaccgggcc gctcggcctg tccgatcgcc
      481 agctggcgga ctgggtcgac cgcgtgcagt ccgcgatctc gtccagcgac tcctccgtgg
      541 tcaaccaggc caccgaggtc ggcacccaga tcggccactt cgtcaccggc ttcttcatcg
      601 cgctgttcgc gctgttcttc ttcctctacg agggcggccg catctggtcg tgggtggtcg
      661 tgctgttccc gaagggcgcg cgtcctcggg tggcctcgtc cggccgcgcc gcgtggggct
      721 cgctcaccgc gttcgtccgc gccacggtcc tcgtcgcgct cgtcgacgcg atcggcatcg
      781 ccggcgtcgc actgatcctg caggtcccgc tcgcggcggc catcggcgtc ctggtgttcc
      841 tcggcgcctt cgtgccgatc gtcggcgcgc tcgtgtcggg catggtcgcc gtcctggtgg
      901 ccctcgtcgc gcaagggccg gtcacggcgc tcctgatgct cgccggcgtg atcgcggtcc
      961 agcagctcga gtcccacgtg ctccagccgt tcctgatggg cgcgatcgtc gccctgcacc
     1021 cgctggccat cctgctggcg atcgccacgg ggctcgtcgt ggccggcgtg gtcggagcgc
     1081 tgctctcggt gccgttcgcg gcgtgcctca acagcgtggt ccggcacctc gcgaacggcg
     1141 agccaccgga caccgacggg gtgctggggg aggagtcccg cgacctcggc gacccaccgc
     1201 ccgccacggc ctagggtgga cgtgtggatc tcgacgatgc gcgcgccgcg cgcgagctgc
     1261 tcgacggagt cagcgaggtc acgccgctga cgcactcccg ctggctgcac gccctcaccg
     1321 ggcgcgacat ccggttcaag tgcgagaacc tccagcgcgc cgggtcgttc aagatccgcg
     1381 gcgcctacac gcgcatcgcg cggctgtccg acgaggagcg ctcccgcggc gtcgtcgccg
     1441 ccagcgccgg caaccacgcg cagggcgtcg ccctcgccgc ggcccagctc ggcgccgacg
     1501 ccacgatctt catgccccac ggcgccgccc tgcccaaggt cgcggccacc cgcggctacg
     1561 gcgccgacat ccgcttccac ggctcgggcg tcaccgaggc gctcgtcgcg gcctcggagt
     1621 tcgccgagga gaccggcgcc accctgatcc acccgttcga ccaccccgac atcgtcgccg
     1681 gccaggccac cgtgggcctc gagatcatcg agcagctgcc cgaggtcgcc acgatcctcg
     1741 tcccgctggg cggtggtggg ctcgcggccg gcatcggcct ggtcaagacc gagcgccccg
     1801 acatcaccgt catcggcgtg caggccgagg ccgccgcggc ctaccccgac tcgctcgccg
     1861 aggggatgcc cgtcgaggcc gatctcggcg ccacgatggc cgacggcatc gccgtggccc
     1921 gccccgggga ggtgcccttc gggctgatcc gcgaccacgt cgacgaggtc gtcacggtct
     1981 cggaggagtc gatgagccag gcgctgatcg gcctgctcga gcgcgccaag ctgctcgtcg
     2041 agcccgccgg tgcggtgggc gtggcggcgc tgctggagca ccccgaccgg ttcgacgggc
     2101 ccgtcgtgcc cgtgctctcc ggcggcaaca tcgacgccct gctgctgctc gaggtgatcc
     2161 gtcacggctt ggcgtccggc ggccgattcc tcatgttccg cgtgcgcatc tccgaccgcc
     2221 ccggcgagct gatgcgcctg ctcaccgcgc tggccgagat ggacgtgaac atcctgaacg
     2281 tgcaccacga ccgcgggtcc gaggccctcg gcgtgcgtga ggtcgaggtc gccctgcagg
     2341 tggccacccg cggtcccgag caccgcgccg acgtgtggcg ccgcctcgtc gagctcggat
     2401 acgacccgat ctgaccccac acgcacgagc g
//
LOCUS       DATMFS010000233         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_169804, whole genome shotgun sequence.
ACCESSION   DATMFS010000233 DATMFS010000000
VERSION     DATMFS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169804"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VNQ33_04240"
     CDS             complement(<1..600)
                     /locus_tag="VNQ33_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97345.1"
                     /translation="MAEPVDQGERAAGASAFLFGRGAVYTVARASQLLAALVVTPLVT
                     RQLSTGAYGTLATASRVSQVVAVVVGLGLPAGLVRIYFDGRGHDRARRLVASTFLVAL
                     AMALVVHLTGPWWSALFRGLPYGAALTAAVWLAVPVCMLEASQGILRAKDDASGFVIA
                     AMTASAGGQLVGLVVVARGGGAAGYLIAVAVVTALAAVLA"
     gene            complement(593..1603)
                     /locus_tag="VNQ33_04245"
     CDS             complement(593..1603)
                     /locus_tag="VNQ33_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97346.1"
                     /translation="MNPENSFYGHRSVLAEAIGSDDPVPRYRAHVQHGWAPFSPVGHP
                     ERVVPSLRYLTWSGGDARTVRSQGIDGVEAVGAPFLYLRRSKGLDGSAPAPRPGDGSM
                     LVYPSHGWEREQMQGNHRRFVDEIRDRAEGPVTVCLYWIEFRQADLRQAYESAGFRVI
                     SHGTRDDPGFLWRQLDELLRHDIVASNHLSTAVWYGGAVGRDLVRFGPEFTMYGEAYR
                     QAWAAYQRRRWPELDERIVAGADAVAMAEAELGADCLRDQAELRGLLAPLDAAARPWR
                     TRARRARTFGEYTGRRTVAVARSRIRPGQIQRRDLETMARGGPAAPAPDTSARPRPSE
                     RG"
     gene            complement(1644..2858)
                     /locus_tag="VNQ33_04250"
     CDS             complement(1644..2858)
                     /locus_tag="VNQ33_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ97347.1"
                     /translation="MARPLRVAHIITKLAVGGAQESALVTLTGLDAEAFDQWLVCGTE
                     VDDEGSLRPAADEAGVRVVDVPCLVRAVSPVADLRAVAALRDHFRRERPDIVHTHSSK
                     AGFIGRAAARSAGIPVVVHSVHGWSFHPEMRPSLRRAVVAAERIAARGTTVLVHEGVP
                     DRAKGLDQGIGDPGQYVLVRNGIDLGAFSAEVDDVEPGQVRRSLGVPDGAWLLGTVGR
                     LADQKAPLAMVDAVAPLMKGSRDLWFVWVGDGPLRDAVAEKAAALGIAPRFRLAGVRR
                     DVPAVLRAMDTFVLSSLWEGLPRTVTEAMASSVPVVATAVDGVAEVVDDGVSGLLVPP
                     HDAGALGRAIQRVREDDGLRARLAAAGVGRAQEFDRSEMLRRLADLYRGLAGGSTPAE
                     VAAGWSSTPAWS"
     gene            complement(2858..>3515)
                     /locus_tag="VNQ33_04255"
     CDS             complement(2858..>3515)
                     /locus_tag="VNQ33_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWJ97348.1"
                     /translation="KLPLGELERASLFFDIGEIHRARYGRVKRLIDLSLGLVGLVPLL
                     LAVPFVWTVNLVGNRGPLLYRQERVGRGGSTFEILKFRTMREAPAAGAGEGAAWTEVD
                     DPRITTFGRVLRTSHLDELPQMVNIVRGQLSVVGPRPEQPHYVRQLSASLPFYGMRHL
                     VRPGLTGWAQVKYGYAGDERDALEKLQYEFFYLRHQGLRFDARIIGRTVRSVLGSEGR
                     "
BASE COUNT          546 a         1331 c         1203 g          435 t
ORIGIN      
        1 ggccagcacc gccgccagcg cggtcacgac cgccaccgcg atcaggtacc cggcagcacc
       61 cccgcctcgc gccaccacca ccagcccgac cagctgcccg cccgcggacg ccgtcatcgc
      121 cgcgatcacg aagcccgagg cgtcgtcttt cgcccgcagg atcccctgcg atgcctcgag
      181 catgcagacg ggcaccgcca gccacaccgc cgcggtgagc gccgctccgt agggcaaccc
      241 gcgaaagagc gccgaccacc acggtccggt gaggtgcacg accagggcca tggccagcgc
      301 caccaggaag gtcgaggcga ccagccggcg ggcgcgatcg tggccgcggc catcgaagta
      361 gatgcggacg agccccgcgg ggagacccag cccgacgacc acggccacca cctggctgac
      421 ccgggaggca gtggcgagcg tgccgtacgc gccggtggac agctggcgcg tcaccaaggg
      481 cgtgacgacg agagccgcca gcagctgcga ggcgcgggcg acggtgtaga ccgccccgcg
      541 cccgaagagg aacgcgctgg cgccggccgc gcgttcgccc tggtcgacgg gttcagccac
      601 gttcggaagg gcgcggtcgg gccgaggtgt ctggcgctgg ggccgcaggg ccgccgcggg
      661 ccatcgtctc gagatcacgc cgctggatct gcccaggacg gatccgagac cgcgccacgg
      721 ccacagttcg gcgccccgtg tactcgccga acgtccgtgc ccgacgggcc ctggtccgcc
      781 aagggcgcgc cgcggcgtcg agcggtgcca ggagcccgcg gagctcggcc tggtcgcgca
      841 ggcagtccgc tccgagctcc gcctccgcca tcgccacggc atcggcgcca gcgacgatcc
      901 gctcgtcgag ctcgggccag cgccgtcgct ggtaggcggc ccacgcctgc cggtaggcct
      961 cgccgtacat cgtgaactcc ggcccgaagc gcaccagatc gcgcccgacc gcccccccgt
     1021 accagacggc cgtcgacaag tggttgctcg ccacgatgtc atggcggagc agctcatcga
     1081 gctgtcgcca gaggaagccc gggtcatccc gggtgccgtg gctgatgacc cggaaaccgg
     1141 cggattcgta ggcctggcgg agatccgcct gacggaactc gatccagtac aggcacaccg
     1201 tcaccgggcc ctccgcccga tcacggatct cgtcgacgaa gcggcggtgg ttgccctgca
     1261 tctgctcgcg ctcccagccg tggctcgggt acaccagcat cgagccgtca cccggacgcg
     1321 gcgccggggc cgatccatcg aggcccttcg accgacgcag gtacaagaac ggggcgccga
     1381 cggcctcgac gccgtcgatt ccctggctcc ggacggttcg ggcgtcgccg ccagaccagg
     1441 tcaggtaccg cagcgagggg acgacgcgct ccgggtgtcc gacgggtgag aacggagccc
     1501 aaccgtgctg gacgtgggcg cggtaccgcg ggacgggatc gtctgagccg atcgcctccg
     1561 ccaggacgct tcgatggccg tagaagctgt tctcggggtt caccgctcct ccatcgaccg
     1621 caggaggccg tggtggaggg ccgtcatgac cacgccggtg tcgacgacca gccggccgcc
     1681 acctccgccg gggtcgatcc acccgccaag cctcggtaga ggtcggcgag ccgccgcagc
     1741 atctcggagc gatcgaactc ctgggcgcgc ccgacgccgg cggccgccaa acgagcgcgc
     1801 agaccatcgt cctcgcggac tcgttggatg gcccgcccga gcgctccggc gtcgtggggc
     1861 ggtaccagca agccggagac cccgtcatcg accacctcgg ccacgccgtc gaccgccgtg
     1921 gccacgacgg gcaccgacga agccatcgcc tcggtcacgg tcctcggtag gccctcccac
     1981 aacgaggaca gcacgaacgt gtccatggcg cgcagcacgg ccggcacgtc gcgccggacc
     2041 cctgcgagcc ggaacctggg cgcgatgccg agcgcggccg ccttctccgc gacggcatcg
     2101 cgcagcggtc cgtcgccgac ccacacgaac cagaggtccc tcgagccctt catcagcgga
     2161 gcgaccgcgt cgaccatcgc cagcggtgcc ttctggtcgg cgagccgtcc cacggtgccc
     2221 agcagccacg cgccgtcggg aacaccgagc gagcgtcgga cctggcctgg ctccacgtcg
     2281 tcgacctcgg cgctgaacgc gcccagatcg atgccgttgc gcaccagcac gtactgccct
     2341 ggatcgccga tcccctggtc gaggcccttg gcacggtccg gcacgccctc gtggaccagc
     2401 acggtggtcc cccgggctgc gatccgctcc gccgcgacca cggcccggcg cagcgacggt
     2461 cgcatctcgg ggtggaagct ccacccgtgg acggagtgga cgacgaccgg gatgcccgcc
     2521 gagcgagccg ccgccctgcc gatgaagccc gccttgctgc tgtgggtgtg aacgatgtcg
     2581 ggccgctccc gccggaagtg gtcgcgaagc gccgccacgg cacgaaggtc ggccaccggc
     2641 gagaccgccc ggacgaggca gggcacgtcg accacccgga cccctgcctc gtcggcggcc
     2701 gggcggaggc tgccttcgtc gtcgacctcg gtcccgcaca ccagccactg gtcgaacgcc
     2761 tcggcatcga gtcccgtcag ggtcacgagc gcgctctcct gcgcaccgcc gaccgccagc
     2821 ttggtgatga tgtgggcaac ccgcagcggc ctcgccatca ccgtccctcc gacccgagca
     2881 cggacctgac cgtacgcccg atgatcctgg catcgaagcg cagcccttgg tggcgaaggt
     2941 agaagaactc gtactggagc ttctcgaggg cgtcacgctc gtctcctgcg tagccgtact
     3001 tcacctgggc ccagccggtg agaccgggcc gcacgaggtg gcgcatgccg tagaacggca
     3061 gcgatgcgct gagctggcgg acgtagtgcg gctgctcggg ccgagggccc accaccgaca
     3121 gctggccgcg cacgatgttc accatctgcg ggagctcgtc gaggtgggag gtccgcagca
     3181 ctcgaccgaa cgtggtgatg cgcgggtcat cgacctccgt ccacgctgcc ccctctccgg
     3241 ctccggcggc tggggcctcc ctcatggtcc ggaacttcaa gatctcgaac gtgctgccgc
     3301 cgcggcccac gcgctcttgg cggtacagca gcggaccgcg gttgccgacc aagttgaccg
     3361 tccagacgaa cggcaccgcg aggagcagcg ggacgagtcc cacgagcccg agggacaggt
     3421 cgatgaggcg cttcacccgt ccgtagcgcg cccggtggat ctcgccgatg tcgaagaaca
     3481 gcgaggcccg ctccagctcc cccaacggca gcttg
//
LOCUS       DATMFS010000234         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_240100, whole genome shotgun sequence.
ACCESSION   DATMFS010000234 DATMFS010000000
VERSION     DATMFS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240100"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2835)
                     /locus_tag="VNQ33_04260"
     CDS             complement(<1..2835)
                     /locus_tag="VNQ33_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWJ97349.1"
                     /translation="MSCVGATVSAEARRVLAADPCGTLNVRLAAAAGRAGGTGIIDVA
                     SPDQLTALAAELARRSVASWWLRPGPAVLPEHVAAAGLEPSIVVLAPVDVDAPAFSSV
                     VDAWAEVGGDVVAQVVSRAEGEAAKAGGVAGLVASGSEAGGRIGDTEAFILFQHLVGL
                     GLPVWVRGGIGRHTAAAVVAGGGAGVVLDAQLGLLREAGLTHDLRRAVEAMDGSETRV
                     VGGHRFLTRPDLPAAAVPEDTPAAEVASFLGSDPRTSLVPIGQDGGFAAGLAARYVTV
                     GGVVQAVQAAIDDHLAAAAADPPLAEGHGVAGPHGTRYPIAQGPMTRVSDRAEFAAAV
                     ADGGGLPFLALALLRGPEVRDLLVQTKELLGDRPWGVGVLGFVPADLRAEQLEVVHDV
                     APPHALIAGGRPSQAAPLEAAGIDTYLHVPSPGLLDRFLKDGARKFVFEGRECGGHVG
                     PRSSFALWDAQIERLLDVPDPDQLQVLFAGGIHDARSAAMAAAAAATLAAKGAHVGVL
                     MGTAYLFTEESVAAGAIQPAFQDVAVTCDTTVLLETAPGHATRCVETDFVRAFANRRD
                     ELIAEGVDPKARWAELESLNLGRLRIASKGLVRDEDGRRDVPPDEQRREGMYMIGEVA
                     TLRHEVTTVAALHDEVSRGSSEWLRPVDDLRLAVREREREADPLDIAIVGMDGFFPGA
                     IGLDQYWAAIVAGTDAVTEVSDERWDVAKYYDADAFTKDAGRKTPSRWGGFLRSVGFD
                     PLAYGIPPASLAAIEPVQLLSLEVASRALADAGYADREFDRSRASVVFGAESGNELAG
                     AYGIRAFLPQIFGDVPAGLEDWLPALTEDSFPGVLANVIAGRVANRLDLGGVNYTVDA
                     ACASSLAALDAACKELRAGTSDLVLCGGADLHNGLNDYLLFASVHALSPTGRCRTFDA
                     NADGIALGEGVACVVLKRRVDAERDGDRIYA"
BASE COUNT          399 a         1031 c         1127 g          317 t
ORIGIN      
        1 cgcgtagatc cggtcgccgt cgcgctcggc gtccacccgg cgcttcaaca cgacgcaggc
       61 gacaccctcc ccgagggcga tgccgtcggc gttggcgtcg aacgtgcggc agcggccggt
      121 gggcgacagc gcgtgcacgg aggcgaacag caggtagtcg ttgaggccgt tgtgcaggtc
      181 ggcgccgccg cacagcacga ggtcgctggt gccggcccgc agctccttgc aggcggcgtc
      241 gagggcggcg agcgaggagg cgcacgccgc gtccaccgtg tagttcacgc cgccgaggtc
      301 gaggcggttg gcgacacggc cggcgatgac gttggcgagc acgccgggga acgagtcctc
      361 ggtgagcgcc ggcagccagt cctcgaggcc ggcggggacg tcgccgaaga tctgcggcag
      421 gaaggcgcgg atgccgtagg caccggccag ctcgttgccg ctctcggcac cgaacacgac
      481 ggacgcacgg gaccggtcga actcgcggtc ggcgtacccg gcatcggcga gggcccggga
      541 ggcgacctcg agcgacagca gctgcaccgg ctcgatggcg gcgagcgacg ccggcgggat
      601 gccgtaggcg agcgggtcga agccgaccga gcggaggaac ccgccccagc gagacggcgt
      661 cttgcggccg gcgtccttcg tgaacgcatc ggcgtcgtag tacttggcga cgtcccagcg
      721 ctcgtcggag acctcggtga ccgcatcggt gccggcgacg atcgccgccc agtactggtc
      781 gagcccgatg gcaccgggga agaacccgtc catgccgacg atggcgatgt cgagcggatc
      841 ggcctcgcgc tcgcgctcgc gcacggcgag gcggaggtcg tcgaccgggc gcagccactc
      901 gctgctgccc cggctgacct cgtcgtgcag ggcggcgacg gtggtgacct cgtggcggag
      961 ggtcgccacc tcgccgatca tgtacatgcc ctcgcggcgc tgctcgtcgg ggggcacgtc
     1021 gcggcgcccg tcctcgtcgc gcaccaggcc cttcgacgcg atgcggaggc ggccgaggtt
     1081 gaggctctcc agctcggccc accgggcctt ggggtcgacg ccctcggcga tgagctcgtc
     1141 gcgccggttg gcgaaggccc gcacgaagtc ggtctcgacg cagcgggtcg cgtgaccggg
     1201 tgccgtctcg agcaggaccg tggtgtcgca ggtgacggcg acgtcctgga acgccggctg
     1261 gatggcaccc gccgccaccg actcctcggt gaagaggtac gcggtgccca tgagcacgcc
     1321 gacgtgagcg cccttggcgg cgagggtggc ggccgcggcc gcggccatgg cggccgagcg
     1381 ggcgtcgtgg atgccgccgg cgaagaggac ctggagctgg tcgggatcgg gcacgtcgag
     1441 caggcgctcg atctgggcgt cccagagggc gaacgaggag cgggggccga cgtggccgcc
     1501 gcactcgcgg ccctcgaaca cgaacttgcg ggcgccgtcc ttcaagaagc ggtccagcag
     1561 gcccggcgac gggacgtgga ggtaggtgtc gatgcccgcc gcctcgagcg gcgcggcctg
     1621 gctgggacgg ccgccggcga tgagcgcgtg cggcggcgcc acgtcgtgca ccacctccag
     1681 ctgctcggcg cggaggtcgg cgggcacgaa gcccagcacg cccacgcccc acggccggtc
     1741 gccgaggagc tccttggtct gcacgaggag gtcgcgcacc tcggggccgc gcaggagcgc
     1801 gagggcgagg aacgggagac cgccgccatc ggccacggcg gcggcgaact cggcccggtc
     1861 gctgacgcgc gtcatcggcc cctgggcgat cgggtagcgg gtgccgtgcg gcccggcgac
     1921 cccgtgcccc tcagccagcg gcgggtcggc ggccgccgca gcgaggtggt cgtcgatcgc
     1981 ggcctgcacg gcctggacca cgccgcccac ggtcacgtag cgagcggcga gaccggcggc
     2041 gaacccgccg tcttggccga tggggaccag cgaggtccgc gggtcggacc ccaggaacga
     2101 ggcgacctcg gccgctggcg tgtcctcggg gacggcggcg gcaggcaggt cgggccgggt
     2161 caggaaccgg tggccgccga ccacccgggt ctcgctgccg tccatggcct cgacggcacg
     2221 gcggaggtcg tgggtgaggc cggcctcgcg cagcagcccg agctgggcgt cgagcaccac
     2281 ccccgcaccg ccaccggcga cgacggcagc ggccgtgtgc cggccgatgc cgccccggac
     2341 ccagaccggc aggccgagcc cgacgaggtg ctggaagagg atgaacgcct cggtgtcgcc
     2401 gatgcggccg ccggcctcgg agcccgacgc gacgagcccg gccacgccgc cagctttggc
     2461 ggcctcgccc tcggcgcggg acacgacctg ggcgaccacg tcgccgccca cctcggccca
     2521 ggcgtcgacg accgacgaga acgccggagc atccacgtcg acgggggcca agacgacgat
     2581 cgacggctcc agcccggccg cggcgacgtg ctcgggcagc accgccggac cgggccgcag
     2641 ccaccacgag gcgaccgagc ggcgggccag ctcggccgcg agcgcggtga gctgatcggg
     2701 cgacgccacg tcgatgatgc cggtgccgcc ggcacgtccg gcggcagcag ccaggcggac
     2761 gttcagcgtg ccgcacggat cggcggccag gacccggcgc gcctcagcgg acacggtggc
     2821 tcccacgcag ctcacgactc ggttcctggc gcacccgatc cccccggaac gtgg
//
LOCUS       DATMFS010000235         4278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_102526, whole genome shotgun sequence.
ACCESSION   DATMFS010000235 DATMFS010000000
VERSION     DATMFS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102526"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..982
                     /gene="coaA"
                     /locus_tag="VNQ33_04265"
     CDS             59..982
                     /gene="coaA"
                     /locus_tag="VNQ33_04265"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HWJ97350.1"
                     /translation="MNSALYETFSRDEWRARRAATPLTLDADDLDELRGLNEALDLDE
                     VESVYLPLSRLVNLHVAAVRGRQDATQVFLDRAQDRVPYVIGVAGSVAVGKSTTARVL
                     RALLARWPDHPRVDLVTTDGFLYPNGELEKRGLLGRKGFPESYDVRRLLQFLRDVKSG
                     VDETRAPTYSHLTYDVLPAEDDIVVRHPDILIVEGLNVLQVSRGTTTFVSDFFDLSIY
                     VDADVTDVRRWYVERFHTLRRTVFTRSDSYFQRYAELSDDEATETALQIWKDINERNL
                     VANIEPTRDRADVILRKGPDHHVDEVRLRRI"
     gene            1068..1853
                     /locus_tag="VNQ33_04270"
     CDS             1068..1853
                     /locus_tag="VNQ33_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWJ97351.1"
                     /translation="MSSRTGTATLGTLVDCWRYPVKSLQGGRTEHLVIGPSGVDGDRT
                     HAVIDPGSGHLLSAKRVSALLHGSATDERITFPDGQVLRFDDPAIDQVLSDWLGRTVH
                     LADEEEAGERSYEMTFDPPNDDAEYFEIPAPPGTFLDLAPVHVVTTATLAGCAAARPG
                     LDWDVRRFRPNLVLDVDIEPFGEQAWLGQQVAVGDVVLVVDSPTVRCAMPLRSQAGGI
                     DREPGLFRAMSELNEAFANHLGLYCSVSEPGTVSVGDPVAVIS"
     gene            1850..2641
                     /locus_tag="VNQ33_04275"
     CDS             1850..2641
                     /locus_tag="VNQ33_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97352.1"
                     /translation="MTISRRVVLAGGLAAAVTACSSGGSDRGGGTSSTATTTTSPGTG
                     SILGDDGTPISDLPRSPRFTAEDFDALGTCVMTPEQTAGPFPLDEQFLRTDITEGVAG
                     TPLRLGFRVTGMAGGACSPTYPASVEVWHADATGDYSAFTDGGGGKDEAEGTTFLRGT
                     QQATNDGIVQFETVYPGWYTGRAVHVHVRVHLGGELALTTQVYFDPDVTAKVYEAEPY
                     AEFGLPDTSWDGDAIAGDPKADGTALALTRTDTGVTGLINLGIPI"
     gene            complement(2662..4059)
                     /gene="sufB"
                     /locus_tag="VNQ33_04280"
     CDS             complement(2662..4059)
                     /gene="sufB"
                     /locus_tag="VNQ33_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWJ97353.1"
                     /translation="MAVTDLDLGKYKLGWSDAEDYVFKPKRGLSTDIVNEMSWMKGEP
                     DWMLARRLKSLKAFDRRPMPNWGGDMSEIYFDDIYYYIKPTDHQVDEWEELPDAIKAT
                     YEKLGIPEAERKYLAGVTAQYESEVVFHRNREDLERLGVIFCDMDTALREYPELVKQY
                     FGTVIPANDNKFSALNTAVWSGGSFIYVPPGVKVDMPLQAYFRINAENMGQFERTLII
                     ADEGSEVHYIEGCSAPTYTSDSLHSAVVEIVVKPSARVTYTTIQNWSNNVFNLVTKRA
                     RVEAEGHMEWIDGNIGSRLTMKYPAVWLVGPKASGEVLSVAYAGSGQHQDTGSKMVHA
                     APETTSKIVSKSISKDGGRTSYRGLIRVEDDAYGCKSHVQCDALILDDISVSDTYPYM
                     EVGARDAVIGHEATVSKVADEQLFYLMSRGLSEDQAMGMVVNGFIEPVTKTLPMEYAV
                     EWSRLIELQMEGSVG"
BASE COUNT          627 a         1536 c         1447 g          668 t
ORIGIN      
        1 ccgccggctc ccgcgggttt ggccccgtgc ggcctccgct ccgtagcttt ggccgggcgt
       61 gaactccgcc ctgtacgaga ccttctcccg tgacgagtgg cgggcgcgcc gagcggccac
      121 gccgctgacc ctcgacgccg acgacctcga cgagctgcgc ggcttgaacg aggcgctcga
      181 cctcgacgag gtggaatccg tctacctccc cctctcccgg ctggtgaacc tccacgtcgc
      241 cgcggtgcgc ggccgccagg atgcgaccca ggtgttcctc gaccgggccc aggaccgggt
      301 gccgtacgtc atcggcgtgg ccggcagcgt ggccgtcggc aagagcacca ccgcccgcgt
      361 gctgcgcgcc ctgctcgccc ggtggcccga ccacccccgc gtcgacctcg tcaccaccga
      421 tggcttcctc tacccgaacg gcgagctcga gaagcggggc ctgctcggcc ggaagggctt
      481 ccccgagagc tacgacgtgc gacggctcct gcagttcctc cgcgacgtga agtcgggcgt
      541 cgacgagacg cgggcgccca cctactcgca cctcacctac gacgtgctgc ccgccgagga
      601 cgacatcgtc gtgcggcacc cggacatctt gatcgtcgag ggtctcaacg tgctccaggt
      661 cagccgcggg accacgacgt tcgtctcgga cttcttcgac ctgtcgatct acgtcgacgc
      721 cgacgtcacc gacgtccgcc gctggtacgt cgagcggttc cacacgctgc gccgcaccgt
      781 gttcacccgg tccgactcgt acttccagcg ctacgccgag ctctccgacg acgaggccac
      841 cgagaccgcc ctccagatct ggaaggacat caacgagcgc aacctggtgg ccaacatcga
      901 gcccacccgt gaccgcgccg acgtcatctt gcgcaagggc cccgaccacc acgtcgacga
      961 ggtccgcctc cgccgcatct gaccccgccc ccagccgagc gggggctcgg ccacctccgt
     1021 gcggtgatcg cccggcaggg gaagccacgt gctggtatcc atggcccatg tcctcccgca
     1081 ccggcacggc aaccctcggc acgctcgtcg actgctggcg ctatccggtg aagtcgctcc
     1141 agggcggtcg caccgagcac ctggtgatcg gcccgtccgg cgtcgacggc gaccgcaccc
     1201 acgccgtcat cgacccgggg tccggtcacc tgctgtcggc caagcgggtg tccgccctgc
     1261 tgcacggatc ggccaccgac gagcgcatca cgttccccga cggccaggtc ctccgcttcg
     1321 acgacccagc catcgaccag gtcctctccg actggctggg ccgcacggtc cacctggccg
     1381 acgaggagga ggccggcgaa cggtcctacg agatgacgtt cgacccgccc aacgacgacg
     1441 ccgagtactt cgagatcccg gcgccgcccg ggacgttcct cgacctggca cccgtgcacg
     1501 tcgtcaccac ggccaccctc gccgggtgcg cggccgcccg gcccgggctc gactgggacg
     1561 tccgccggtt ccgcccgaac ctcgtgctcg acgtggacat cgagccgttc ggcgagcagg
     1621 cgtggctcgg gcagcaggtc gccgtgggcg acgtcgtgct ggtcgtcgac agccccacgg
     1681 tccgctgcgc catgccgctg cggtcccagg ccggcgggat cgaccgcgag cccgggctgt
     1741 tccgggcgat gagcgagctg aacgaggcct tcgcgaacca cctcggcctg tactgctccg
     1801 tgtccgagcc cggcaccgtg tcggtcggcg accccgtggc ggtgatctcg tgaccatctc
     1861 ccggcgggtg gtcctcgccg gcgggctcgc cgcggccgtc accgcgtgca gcagcggcgg
     1921 gagcgaccgg ggcggcggaa cgtcatcgac cgcgaccacg accacgtcac cggggaccgg
     1981 ttcgatcctg ggcgacgacg ggaccccgat cagcgacctc ccccgctccc cccgcttcac
     2041 cgccgaggac ttcgatgccc tcggcacctg cgtcatgacg cccgagcaga cggcgggccc
     2101 gttcccgctc gatgagcagt tcctccgcac ggacatcacc gagggcgtcg ccgggacgcc
     2161 gctgcgcctc gggttccggg tgacgggcat ggccggcggc gcgtgcagcc cgacctaccc
     2221 ggcgtccgtg gaggtgtggc acgccgacgc caccggcgac tactcggcgt tcacggacgg
     2281 cggcggcggc aaggacgagg ccgaggggac cacgttcctc cgcggcaccc agcaggcgac
     2341 caacgacggc atcgtgcagt tcgagacggt ctaccccggt tggtacacgg ggcgggcggt
     2401 ccacgtccac gtgcgggtgc acctcggcgg cgagctcgcg ctcaccacgc aggtgtactt
     2461 cgacccggac gtgacggcga aggtctacga ggccgagccc tacgccgagt tcggcctgcc
     2521 cgataccagc tgggacggcg acgccatcgc cggcgacccg aaggccgacg gcaccgcgct
     2581 ggcgctcacc cggaccgaca ccggcgtgac cggcctcatc aacctcggca tcccgatctg
     2641 acgagacgcc caggcggccg gtcagccgac cgagccctcc atctggagct cgatgaggcg
     2701 ggaccactcg acggcgtact ccatcgggag ggtcttggtc accggctcga tgaagccgtt
     2761 gacgaccatg cccatcgcct ggtcctcgga gaggccgcgg ctcatcaggt agaagagctg
     2821 ctcgtcggcc accttggaga cggtggcctc gtggccgatg acggcgtcgc gggcgccgac
     2881 ctccatgtag gggtaggtgt ccgacaccga gatgtcgtcg aggatgaggg cgtcgcactg
     2941 gacgtggctc ttgcagccgt aggcgtcgtc ttcgacccgg atgaggccgc ggtacgaggt
     3001 ccggccgccg tccttggaga tcgacttcga cacgatcttg gaggtggtct cgggcgcggc
     3061 gtggaccatc ttcgagccgg tgtcttggtg ctggccgctg ccggcgtagg cgaccgagag
     3121 gacctcgccc gaggccttcg ggccgacgag ccagaccgcc gggtacttca tggtgaggcg
     3181 gctgccgatg ttgccgtcga tccactccat gtggccctcg gcctcgaccc gggcccgctt
     3241 ggtgaccagg ttgaagacgt tgttcgacca gttctggatg gtggtgtagg tgacgcgggc
     3301 agacggcttg accacgatct cgacgaccgc ggagtgcagc gagtcggagg tgtacgtcgg
     3361 agcggagcag ccctcgatgt agtgcacctc ggaaccctcg tcggcgatga tcagcgtgcg
     3421 ctcgaactgg cccatgttct ccgcgttgat gcggaagtag gcctgcagcg gcatgtcgac
     3481 cttgacgccc ggcgggacgt agatgaacga gccgcccgac cacacggccg tgttgagcgc
     3541 cgagaacttg ttgtcgttgg ccgggatgac ggtcccgaag tactgcttga ccagctccgg
     3601 gtactcccgc agggcggtgt ccatgtcgca gaagatcacc ccgagccgct cgaggtcctc
     3661 ccggttgcgg tggaagacga cctcggactc gtactgggcg gtgacgcccg cgaggtactt
     3721 gcgctcggcc tcggggatgc ccagcttctc gtaggtcgcc ttgatggcgt cgggcagctc
     3781 ctcccactcg tcgacctgat ggtccgtggg cttgatgtag tagtagatgt cgtcgaagta
     3841 gatctccgac atgtcgccgc cccagttggg catcgggcgg cggtcgaagg ccttcaggct
     3901 cttgaggcgc cgggcgagca tccagtcggg ctcgcctttc atccacgaca tctcgttcac
     3961 gatgtcggtg ctcaggccgc gcttcggctt gaagacgtag tcctcagcat cggaccagcc
     4021 gagcttgtac ttgccgagat cgagatcggt gactgccatg acgtggttcc ccaggaggac
     4081 gagcgaattt ctcctacgcc gatggtagca attccacctc cccgatccac agggcggcgg
     4141 tcaggcgtgg gcggcgccct cggaggcttc gatgatgcgg tagcagtcga cgcggtcgag
     4201 gtggacctcg agcgctcgcc gcgcgtcccg gagccgctgc ggggtctggg tgccgaggat
     4261 ggacaccgga cccgacgg
//
LOCUS       DATMFS010000236         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_148425, whole genome shotgun sequence.
ACCESSION   DATMFS010000236 DATMFS010000000
VERSION     DATMFS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148425"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1107
                     /locus_tag="VNQ33_04285"
     CDS             172..1107
                     /locus_tag="VNQ33_04285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWJ97354.1"
                     /translation="MGTVLPPGRLAVGMQLPIQSKSTNFVEPWEEHAGAAELAAVAQA
                     ADRAGFFYVGVCDHIAIPSEKAGVMSTEWWDTVATLAWLAGITTNVRLLSHIYVPAYR
                     HPLQVAKSFATIDAISRGRVIMGVGAGHVEAEFELLGLPFAERGTLLDESIDAVRAAF
                     AEDYPTLPGPVWPAADLGQHPRPVQEDGPPIWVGGSSPAALRRAAQRGDGWLPQGPVT
                     PQVISQIIALRDEAGRHDAFDFGALAGPVYVGEADWDLGSPTLCGPPEKIAHVLRKFQ
                     AMGVGQVQVRLRSRSVEELLDQIDRFGAEVIPQLG"
     gene            1138..1920
                     /locus_tag="VNQ33_04290"
     CDS             1138..1920
                     /locus_tag="VNQ33_04290"
                     /inference="COORDINATES: protein motif:HMM:NF005909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97355.1"
                     /translation="MLLEGKVAIVSGIGPGMGRDISLAFAREGARVALGGRTLSRLEA
                     VAEEVEALGVRALPVVCDIADEAACTEAVNHAAAELGRLDILVNNAYDGGDAKLFMDA
                     DFDRWRQTIEVNLFGSLAMTRAAVPHLEATGDGRVIMINTMSVQRIQERWGSYAASKG
                     ALATVTKTLALELGPKGIRVNGIHPGYIYGDSVEWYFNHQAEKRGVTFQEVYDDVASE
                     TCLKYLPHSEEIAGTAVYFASPLAKPVTGQMIGVNAGHWFNG"
     gene            1944..2297
                     /locus_tag="VNQ33_04295"
     CDS             1944..2297
                     /locus_tag="VNQ33_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HWJ97356.1"
                     /translation="MKLFAFVKRKEGMSREEFLAYWHTQHGPMIRDTPELGGQTLSYV
                     QYPAAPQDQSGWDGVAVQEFASWGDFVAMLSGPAGDTMREDEANFLDSSSIKVVFTEG
                     QVVMIDPADPPAARP"
     gene            2294..3088
                     /locus_tag="VNQ33_04300"
     CDS             2294..3088
                     /locus_tag="VNQ33_04300"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ97357.1"
                     /translation="MSGRFEGRQALVTGGGSGIGEAVCRLFASEGAAVTVLDRDGGAA
                     DRVAADIGGWGVACDVRDADGVNTAVLDAASAMGGLTDLVNNAGLGTAKPLLSYTDKE
                     WALLIGVNLTGTFHGIRAAGPIMLEAGRGSIVNNASLTGVRPTRGEGPYSAAKAGVLN
                     LTQSAALELAPAVRVNAVAPGMIHTPLTDIVVQNDEWRTAAESGTPAGRVGGADEVAE
                     VIAFLSSDAASYVTGQTIVVDGGSVLPSLQSDRLLRAISESGFGLG"
     gene            3100..3561
                     /locus_tag="VNQ33_04305"
     CDS             3100..3561
                     /locus_tag="VNQ33_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWJ97358.1"
                     /translation="MLTDADHIRNLIGRSCDLMDAGDFEGIGALFVEASLAAGEGEPF
                     AQGPDEIAAFFARVTQRHDGALGTKHLVTNTVMGEPDASGSVLARSSYLVLQAVDGLP
                     LQPIITGRYHDRFAKGAGGWYFTERRFFVDLAGDLSHHLADPSIAGPGAAS"
     gene            3558..3683
                     /locus_tag="VNQ33_04310"
     CDS             3558..3683
                     /locus_tag="VNQ33_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97359.1"
                     /translation="MSGLDEVLRSMPTGVGPRFGQGPDGVLAASVEEPPLRWSGP"
     gene            3680..>4113
                     /locus_tag="VNQ33_04315"
     CDS             3680..>4113
                     /locus_tag="VNQ33_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ97360.1"
                     /translation="MTAPRPSATWITPLDPDLDPLARPDGLRLAIKDCIDVAGVVTTA
                     GSPLIAERAEPATVDAPCLEVARAAGARIVGKANLHELCFGATGVNPHYGTPVNPRDP
                     RRVPGGSSSGSAVAVADREADIGFGTDTAGSIRNPAASCGIVG"
BASE COUNT          583 a         1482 c         1466 g          582 t
ORIGIN      
        1 acgccaacca ggagtcgttc acccacggca cctcggccca gcgcaagcag tggttcacga
       61 ccggcttcga gtccggcgac gccggccgct gcgacacgtt cggctgactc gcccgcaccc
      121 tcagcggtga aactgcaaca cgttctagtt ttctgacgta gggtccgggc gatggggacc
      181 gtcttgcctc cgggacggct ggccgtgggc atgcagctcc cgatccagtc gaagtcgacc
      241 aacttcgtgg agccgtggga ggagcacgcc ggtgccgccg agctggccgc ggtcgcccag
      301 gccgccgacc gggccgggtt cttctacgtg ggggtgtgcg accacatcgc catcccctcg
      361 gagaaggccg gggtcatgag cacggagtgg tgggacaccg tcgccacgct cgcctggctg
      421 gcgggcatca cgacgaacgt gcgcctcctc agccacatct acgtgcccgc ctaccgccac
      481 cccctccagg tggcgaagtc gttcgccacc atcgacgcca tctcccgcgg ccgggtgatc
      541 atgggcgtcg gtgctggcca cgtggaggcc gagttcgagc tgctcggcct gccgttcgcg
      601 gagcggggca cgctgctcga cgagtccatc gacgccgtgc gcgccgcctt cgccgaggac
      661 tacccgacgc tgcccggacc ggtgtggccg gcggccgacc tcggccagca cccgcgtccg
      721 gtccaggagg acggcccgcc gatctgggtc gggggttcgt cacccgcggc cctgcggcgc
      781 gccgcccagc gcggcgacgg ctggctgccg caggggccgg tcaccccgca ggtcatcagc
      841 cagatcatcg cgctgcgcga cgaggccggc cgccacgatg ccttcgactt cggcgccctg
      901 gccgggccgg tgtacgtcgg cgaggccgac tgggacctcg gttcccccac gctgtgcgga
      961 ccgcccgaga agatcgccca cgtgctgcgg aagttccagg cgatgggcgt gggccaggtc
     1021 caggtccggc tgcgctcgcg ctcggtcgag gagctcctcg accagatcga ccgcttcggc
     1081 gccgaggtca tcccccaact cggctgaacg ccgcacccac ggatcaggag tcactgcatg
     1141 ctgctcgaag gcaaggtcgc catcgtctcg gggatcggcc ccggcatggg ccgggacatc
     1201 tcgctcgcgt tcgcccggga gggcgcccgc gtggcgctcg ggggccggac gctgagccgg
     1261 ctggaggcgg tggccgagga ggtcgaggcg ctcggcgtcc gcgccctgcc ggtggtgtgc
     1321 gacatcgccg acgaggccgc ctgcaccgag gcggtgaacc acgcggccgc cgagctgggc
     1381 cggctcgaca tcctcgtcaa caacgcctac gacggcggcg acgccaagct cttcatggac
     1441 gccgacttcg accgctggcg ccagaccatc gaggtcaacc tgttcggctc cctcgccatg
     1501 acccgcgccg cggtgccgca cctcgaggcg accggcgacg gccgggtgat catgatcaac
     1561 acgatgtcgg tgcagcggat ccaggagagg tggggctcgt acgcggcgtc caagggggcg
     1621 ctggccaccg tcaccaagac gctggcgctg gagctcgggc cgaaggggat ccgggtcaac
     1681 ggcatccacc ccggctacat ctacggcgac tcggtcgagt ggtacttcaa ccaccaggcc
     1741 gagaagcggg gggtcacgtt ccaggaggtc tacgacgacg tggcgtcgga gacgtgcctc
     1801 aagtacctgc cgcactccga ggagatcgcc ggtaccgccg tgtacttcgc atcacccctg
     1861 gccaagccgg tcaccggcca gatgatcggc gtcaacgccg gccactggtt caacgggtga
     1921 cctcgtccga aggaggacct gcgatgaagc tgttcgcgtt cgtgaagcgc aaggagggga
     1981 tgtcccgcga ggagttcctc gcctactggc acacccagca cgggccgatg atccgcgaca
     2041 cccccgagct gggcgggcag acgctcagct acgtgcagta cccggcggcg ccgcaggacc
     2101 agtcgggctg ggacggcgtc gccgtgcagg agttcgcctc ctggggcgat ttcgtcgcca
     2161 tgctctcggg cccagctggc gacacgatgc gcgaggacga ggccaacttc ctcgactcgt
     2221 cgtcgatcaa ggtcgtgttc accgagggcc aggtcgtcat gatcgatccc gcagacccgc
     2281 cggcggctcg accgtgagcg ggcggttcga ggggcgccag gcgctcgtga ccggcggtgg
     2341 ctcggggatc ggcgaggcgg tgtgccgcct gttcgcatcc gaaggtgctg ccgtcaccgt
     2401 cctcgaccgc gacgggggag cggccgaccg ggtggccgcc gacatcggcg ggtggggcgt
     2461 ggcgtgcgac gtgcgcgacg ccgacggggt caacaccgcg gtgctggacg cggcgtcggc
     2521 catgggcggg ctgacggatc tggtgaacaa cgcggggctg ggcacggcca aaccgctgct
     2581 cagctacacc gacaaggagt gggcactgct gatcggcgtg aacttgaccg ggaccttcca
     2641 cggcatccga gccgcgggtc cgatcatgct cgaggcagga cggggctcga tcgtcaacaa
     2701 cgcctcgctc accggcgtcc ggccgacccg gggagagggc ccgtactcgg cggccaaggc
     2761 cggcgtgctc aacctcacgc agtcagccgc gctcgagctg gcgccggcgg tgcgggtgaa
     2821 cgccgtcgcc ccggggatga tccacacgcc cttgaccgac atcgtcgtgc agaacgacga
     2881 gtggcggacg gccgccgagt ccggcacgcc agcgggtcgc gtcggtggtg cggacgaggt
     2941 cgccgaggtg atcgccttcc tctccagcga tgccgcctcc tacgtgaccg gccagaccat
     3001 cgtcgtcgac ggcggatcgg tgctgccgag cctccagtcc gaccgcctct tgcgggccat
     3061 ctccgagtcc ggcttcgggc tgggttgatc ggatcccccg tgctgaccga cgccgaccac
     3121 atccgcaacc tcatcggccg ctcctgcgac ctgatggacg cgggcgactt cgagggcatc
     3181 ggcgccctct tcgtcgaagc gtcgctcgcc gcgggggagg gggagccgtt cgcccagggc
     3241 ccagacgaga tcgccgcctt cttcgccagg gtcacgcagc gccacgacgg tgcgctcggc
     3301 acgaagcacc tggtcacgaa caccgtgatg ggggagcccg acgcctcggg gagcgtgctg
     3361 gcccggtcgt cgtacctcgt gctgcaggcg gtcgacgggc tcccgctgca gccgatcatc
     3421 accggccgct accacgaccg cttcgccaag ggcgctggtg gctggtactt caccgagcgc
     3481 cggttcttcg tcgacctcgc cggggacctg tcgcaccacc tcgccgaccc ctcgatcgcc
     3541 ggacccggcg ccgcgtcgtg agcgggctgg acgaggtcct gcggtcgatg cccaccggcg
     3601 tcggccctcg cttcggccag ggacccgacg gcgtgctcgc tgcctccgtc gaggagccgc
     3661 cgctccgctg gagcgggccg tgaccgctcc ccggccctcg gcgacctgga tcacccccct
     3721 cgacccggac ctcgacccgc tcgcccggcc cgacgggctc cgcctggcga tcaaggattg
     3781 catcgacgtc gccggggtcg tcaccaccgc cggatcgccc ctcatcgccg agcgcgccga
     3841 gccggcgacg gtcgatgccc cgtgcctgga ggtggcgcgg gccgcggggg cgcgcatcgt
     3901 gggcaaggcc aacctccacg agctgtgctt cggggccacg ggcgtcaacc cgcactacgg
     3961 cacgccggtg aacccgcggg atccccgccg ggtgcccggc ggctcatcga gcggatcagc
     4021 cgtggcggtg gccgatcggg aggccgacat cgggttcggg accgacacgg cgggatcgat
     4081 ccgcaacccg gcggcctcct gcggcatcgt ggg
//
LOCUS       DATMFS010000237         3446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_169834, whole genome shotgun sequence.
ACCESSION   DATMFS010000237 DATMFS010000000
VERSION     DATMFS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169834"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNQ33_04320"
     CDS             <1..392
                     /locus_tag="VNQ33_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97361.1"
                     /translation="GPAELAARTRLQTLTRHQVGRLPPALQAEHAEWVASTSATPTAE
                     ERRTRARRLALSLLELGEPDEAVELELHRWKVHPGMAHEALRWAHARRAGTEAAPSAA
                     ALATAAAAPIPPPRRPRPVYDPADDVA"
     gene            complement(438..1901)
                     /locus_tag="VNQ33_04325"
     CDS             complement(438..1901)
                     /locus_tag="VNQ33_04325"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWJ97362.1"
                     /translation="MPVAVALDHLAALPADPPEGAVVAVGVRADRLADDAPGLDLDLA
                     RISGFEGKVGQLLVAATDAGTRLLVGLGKEPDPTTFRRIGASISKRAASQASLLVDLL
                     GHLDGADRAAAAEALALGLILGTYRFGTYKPAPVGTALASISVVAKGGARSAGAVERG
                     VAVATAVVLVRDLVNTPGGDLTPKAFAAEAAKVARDQGLEIEVLDQAAIRKAKLGGLL
                     GVNRGSAQEPRFVKVVHRPEGRAKGKVALVGKGVTFDAGGLSIKTASGMEWMKTDMAG
                     AATVLGAMSLVPTVAPKLEVTAYLPLTDNMLGPDATRVGDVLTARNGKTIEVLNTDAE
                     GRLILADGLSLASEDEPDAIIDLATLTGAVVVALGERTAGLMTNHEALRDRVLAAAEA
                     AGEAVWPLPLPDHLRKGLDSEVADLRNIGTGPYGGALTAALFLREFVGEVPWVHLDIA
                     GPADTTSAWDENARGGTGFGVRTLVNLLSAWSKLPSA"
     gene            complement(1942..2478)
                     /locus_tag="VNQ33_04330"
     CDS             complement(1942..2478)
                     /locus_tag="VNQ33_04330"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWJ97363.1"
                     /translation="MKIYTRKGDDGTTGLYGGGRVPKDSAAPEAYGTVDEAQSALGLA
                     RAAAGRGSELDGLLVHLERDLWVLMAELATEHENARKLVDGATRVTAEMVGVLEEKID
                     ELTARFEQPTEFVVPGETELAARLDVARTVIRRAERRSMAVAADGSQVVPYLNRLSDL
                     VWTLARWSEGTSVETRSV"
     gene            2577..>3446
                     /locus_tag="VNQ33_04335"
     CDS             2577..>3446
                     /locus_tag="VNQ33_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWJ97364.1"
                     /translation="MTSSPFLDLLADRVVVYDGATGTWLQTQDLSLDDYGGEAFEGCT
                     DILGVTRPDVIAALHTAYLEVGADVVETNTFGAFGVPLGEYDIPERAHELCVANARIA
                     REVADGFSTPDRKRYVAGSLGPGTKSPSLSQIRFAELRDHYQVAAQGLLEGGVDLFIL
                     ETHFDLLAVKAAVIGVRRAMAKVGRQVPIQCQVTMELTGRMLVGTEISAALAAIDPLK
                     VDIIGLNCATGPTEMSEHIRHLSQHSRVPISVLPNAGLPSVVDGKMHYDLTPEQMEAH
                     QRRFVTELGVQVIG"
BASE COUNT          469 a         1306 c         1228 g          443 t
ORIGIN      
        1 ccgggcccgc cgagctggcg gcgcggaccc ggctgcagac gctgacccgc caccaggtcg
       61 gccggctgcc accggcgctc caggccgagc acgccgagtg ggtggcctcc accagcgcga
      121 cgccgaccgc cgaggagcgg cggacccggg cccggcggct ggccctcagc ctgctggagc
      181 tgggtgagcc cgacgaggcc gtcgagctcg agctgcaccg ctggaaggtc cacccgggca
      241 tggcccacga ggccctgcgc tgggcgcacg cccgccgagc cggcaccgag gccgcaccct
      301 cggcggccgc cctggccacc gcggccgcgg ctcccatccc accgcctcga cggccccgcc
      361 cggtctacga cccggccgac gacgtcgcct gacgcggtgg ctgcggcgca cccgccagcc
      421 gggcgccgcc gccggggtca ggcactcggg agcttcgacc aggcggacaa gaggttgacg
      481 agggtccgca ccccgaagcc ggtgccgccc cgggcgttct catcccaggc cgacgtggtg
      541 tcggcaggcc cggcgatgtc gaggtggacc cacggcacct cgccgacgaa ctcgcggagg
      601 aacagcgccg cggtgagcgc cccgccgtag gggccggtgc cgatgttgcg gagatccgcc
      661 acctcgctgt cgaggccctt gcgcaggtgg tcgggcaggg gcagcggcca caccgcctca
      721 ccggcggcct cggcggcggc gagcacccgg tcgcgcagcg cctcgtggtt ggtcatgagg
      781 cccgccgtgc gctcgccgag ggcgacgacg acggcgccgg tgagcgtcgc caggtcgatg
      841 atcgcatcgg gctcgtcctc ggaggcgagc gacaggccgt cggcgaggat caggcggccc
      901 tcggcatcgg tgttgaggac ctcgatcgtc ttgccgttgc gggcggtgag cacgtcgccg
      961 acgcgggtgg cgtcggggcc gagcatgttg tcggtgagcg ggaggtaggc ggtgacctcg
     1021 agcttgggcg ccaccgtcgg gaccaggctc atggcgccga ggacggtggc ggcaccggcc
     1081 atgtcggtct tcatccactc catgcccgac gcggtcttga tgctgaggcc gccggcatcg
     1141 aacgtgacgc ccttgcccac gagcgccacc ttgcccttgg ccctcccctc cggccggtgc
     1201 acgaccttca cgaagcgggg ctcttgcgcc gaaccccggt tgacgccgag caggccgccg
     1261 agcttggcct tgcggatggc ggcctggtcg agcacctcga tctcgaggcc ctggtcgcgg
     1321 gccaccttgg cggcctcggc ggcgaacgcc ttgggcgtga ggtcgccgcc gggcgtgttc
     1381 accaggtccc ggaccagcac cacggccgtg gcgacggcga cgccgcgctc gaccgcgccc
     1441 gcggatcggg caccgccctt ggccacgacc gagatcgagg ccagcgccgt accgacgggc
     1501 gccggcttgt aggttccgaa ccggtaggtg ccgaggatca gccccagcgc cagcgcctcg
     1561 gccgcggcgg cgcgatcagc gccgtcgagg tgaccgagca ggtcgaccag gagcgatgcc
     1621 tgcgaggcgg cccgcttgct gatggaggcg ccgatgcggc ggaacgtcgt cgggtcgggc
     1681 tccttgccca ggcccaccag caggcgggtg cccgcatcgg tcgcggccac cagcagctgg
     1741 ccgaccttgc cctcgaaccc gctgatgcgg gcgaggtcca agtcgaggcc gggggcgtcg
     1801 tccgcgaggc ggtccgctcg cacgccgacg gcgacgacgg cgccctccgg cgggtcggcg
     1861 ggcagggccg cgaggtgatc gagggcgacg gcgacaggca tggcccgcag cgtacggcgc
     1921 accgtgccga cgatcgcccg atcagaccga gcgggtctcc accgacgtgc cctcgctcca
     1981 ccgggccagg gtccagacca gatcggacag ccggttgagg tacggcacga cctgcgagcc
     2041 gtccgcggcc accgccatcg accggcgctc ggcgcggcgg atgacggtcc gggcgacgtc
     2101 gagccgggcg gccagctcgg tctcccccgg cacgacgaac tcggtgggct gctcgaagcg
     2161 ggccgtgagc tcgtcgatct tctcctcgag caccccgacc atctcggcgg tcacccgggt
     2221 ggcgccgtcc acgagcttgc gggcgttctc gtgctcggtg gccagctccg ccatcagcac
     2281 ccacaggtca cgctcgaggt ggaccaggag gccgtcgagc tcgctgcccc tgcccgcggc
     2341 cgcccgggcc agaccgagcg cggactgggc ctcgtcgacc gtgccgtagg cctccggcgc
     2401 cgccgagtcc ttgggcacgc gccctcctcc gtagagaccc gtggtgccgt cgtcgccctt
     2461 gcgggtgtag atcttcatgc ccggccgaca ctaccggcca cctgcagacg ccggcccggg
     2521 gccgggcaca ggtatcggac ttacggcacg tcgggacgcg ccagtagctt gaacccgtga
     2581 cctcttcgcc cttcctggac ctcctagccg atcgcgtcgt cgtgtacgac ggcgccaccg
     2641 gcacctggct gcagacccaa gacctgagcc tcgacgacta cggaggcgag gcgttcgagg
     2701 gctgcaccga catcttgggc gtcacccgcc ccgacgtcat cgccgccctg cacaccgcgt
     2761 acctcgaggt cggcgccgat gtggtcgaga ccaacacgtt cggcgcgttc ggcgtgccgc
     2821 tcggcgagta cgacatcccc gagcgggccc acgagctgtg cgtggccaac gcccgcatcg
     2881 cccgggaggt ggccgacggc ttctccacac cggaccgcaa gcgctacgtg gccggctccc
     2941 tcgggccggg caccaagtcg ccgtcgctca gccagatccg cttcgccgag ctgcgcgacc
     3001 actaccaggt cgccgcccag ggcctgctcg agggcggggt cgacctgttc atccttgaga
     3061 cccacttcga cctgctcgcc gtgaaggcgg cggtcatcgg cgtccgccgg gccatggcca
     3121 aggtcggccg ccaggtgccg atccagtgcc aggtcacgat ggagctcacc ggccgcatgc
     3181 tcgtgggcac cgagatcagc gccgcgctcg ccgccatcga cccgctgaaa gtcgacatca
     3241 tcggcctcaa ctgcgccacc ggccccaccg agatgagcga gcacatccgc cacctcagcc
     3301 agcacagccg ggtgccgatc tccgtcctcc cgaacgccgg ccttccgagc gtcgtcgacg
     3361 gcaagatgca ctacgacctc acaccggagc agatggaggc ccaccagcgg cggttcgtca
     3421 ccgagctcgg cgtccaggtc atcggc
//
LOCUS       DATMFS010000238         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_32340, whole genome shotgun sequence.
ACCESSION   DATMFS010000238 DATMFS010000000
VERSION     DATMFS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32340"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VNQ33_04340"
     CDS             <1..774
                     /locus_tag="VNQ33_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWJ97365.1"
                     /translation="QRLFAEAYEGHPYIHVPAVVDELSSTRVLTTELAPGVRFADMLT
                     WGQEERNLAAETIYRFVFGSLYRLSAFNGDPHPGNYLFERGGKVTFLDYGLVKRFTSE
                     ELQPFEDMIKAMVIDEDVAEFRRIVEAVGLLTPNPAFTDEAIGDYFGHFYEFVRDDAE
                     LTMTEAYASETVRRFFDTSGEHGEIMRAANVPKSMVIIQRINLGLYAILGQLEATANW
                     RRLAEELWPWTDGAPSTPMGEAEAAWRAERAAARSIPAD"
     gene            complement(771..1244)
                     /locus_tag="VNQ33_04345"
     CDS             complement(771..1244)
                     /locus_tag="VNQ33_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97366.1"
                     /translation="MTASTTEPAPPAPAAAPARPGPTGIVEATAIIAAIGVVVALIGL
                     GILLLPVTTPTQDCGTSAGFLLGGRVDQMVSETDPPAGVTAAEAKANNASPCRERVAD
                     RAKPAALLLGTGLIAALGAALAEMAIRLVRRSKRRRAGAAPQPRAADDRSAPAAS"
     gene            1604..1846
                     /locus_tag="VNQ33_04350"
     CDS             1604..1846
                     /locus_tag="VNQ33_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97367.1"
                     /translation="MLTLLAASDNGGAFLGDDLLPYLVLALGGALVAGNVAAIVKPPK
                     QHRGEDDLERAPMGRSVFMAVLGAIAAVWALASLLT"
     gene            complement(1872..2309)
                     /locus_tag="VNQ33_04355"
     CDS             complement(1872..2309)
                     /locus_tag="VNQ33_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ97368.1"
                     /translation="MDAGEVIERLGLVPHPEGGWYRETWRDLPADGGRGVGSAIYFLL
                     DGHERSHWHRVDAAEAWHHYAGAPVELAIAEDDDHAVLTLGPDLGAGQEPQAVVPARA
                     WQAARPLGGWALVGCTVSPAFDFSGFELATEGWAPDGWDRPGW"
BASE COUNT          338 a          885 c          925 g          344 t
ORIGIN      
        1 cagcggctct tcgccgaggc ctacgagggc cacccgtaca tccacgtccc ggccgtggtc
       61 gacgagctga gctcgacccg ggtgctgacc accgagctgg cgcccggcgt ccgcttcgcc
      121 gacatgctca cgtggggcca agaggagcgc aacctcgccg ccgagaccat ctaccggttc
      181 gtgttcggca gcctctaccg cctctcggcg ttcaacggcg acccgcaccc cggcaactac
      241 ctcttcgagc gcgggggcaa ggtcacgttc ctcgactacg gcctcgtcaa gcggttcacc
      301 tccgaggagc tgcaaccgtt cgaggacatg atcaaggcga tggtgatcga cgaggacgtc
      361 gccgagttcc ggcgcatcgt cgaggcggtc ggcctgctca cgccgaaccc ggcgttcacc
      421 gacgaggcga tcggcgacta cttcggccac ttctacgagt tcgtccgaga cgacgccgag
      481 ctcaccatga ccgaggcgta cgcgagcgag acggtgcgcc gcttcttcga cacgtcgggc
      541 gagcacggcg agatcatgcg ggccgccaac gtgcccaagt cgatggtgat catccagcgg
      601 atcaacctcg ggctctacgc catcttgggc cagctcgagg ccacggcgaa ctggcggcgg
      661 ctcgccgagg agctgtggcc gtggaccgac ggcgcgccgt cgacgccgat gggcgaggcc
      721 gaggctgcct ggcgcgccga gcgggcggcg gctcgctcga tcccggctga ctaggaggca
      781 gcgggcgccg agcggtcgtc ggcggcccgt ggctggggcg cggcgccggc acggcggcgc
      841 ttcgaccggc ggacgagccg gatcgccatc tccgccaggg cggcgccgag ggcggcgatg
      901 agccccgtcc cgaggagcag cgcggcgggc ttggcgcggt cggccacccg ctcccggcac
      961 ggggacgcgt tgttggcctt ggcctcggcc gcggtgaccc ccgcgggcgg gtcggtctcg
     1021 ctgaccatct gatcgacccg cccgccgagc aggaaccccg ccgaggtccc gcagtcttgg
     1081 gtgggcgtgg tgaccggcag cagcaggatc ccgagcccga tcagcgccac gacgaccccg
     1141 atggcggcga tgatcgccgt cgcctcgacg atgcccgtcg gcccggggcg cgccggtgcg
     1201 gcggcaggag ccggcggcgc gggttcggtg gtggaagcgg tcacggcgtc ctcatgaggc
     1261 ggggcgggtc gccagcgtag gtctggaccg gccggacccg gacgcgccac cgcctcccag
     1321 aggggaggcg gtgggctggc actgcggtgg gtgtgtctgg ggtggagcgg aagccaccac
     1381 agtgctgtgt gccctggaca agcacaccga gcattgcgtg atggagggag gaaagtggct
     1441 cccgctcctg aaccccgtct gaccggtggg ggcgatggtg ccaccggcat ctcaaccatc
     1501 ggcggcgcac ctgcggaact tgagcagatt ttcacgcttc gtgtcgggaa aagtgctcag
     1561 agtgaggagg ttgtcagaac cgggcgagca gtaccctgcg gaggtgctca ccctgctcgc
     1621 cgccagcgac aacggcggtg ccttcctcgg cgacgacctg ctcccctacc tcgtgctggc
     1681 cctgggcggt gcgctggtgg ccggcaacgt cgccgccatc gtgaagccgc cgaagcagca
     1741 ccggggcgag gacgatctcg agcgggcccc catgggccgc tcggtcttca tggccgtgct
     1801 cggcgcgatc gcggccgtgt gggcgctcgc ttccctgctg acctgagccg acgcgacctc
     1861 gaccggcctg gtcaccaacc aggtcggtcc cacccgtcgg gggcccagcc ctcggtggcg
     1921 agctcgaacc cggagaagtc gaacgcgggg ctgaccgtgc agcccaccag cgcccagccg
     1981 cccaggggcc gggcggcctg ccaggcccgt gccgggacca ccgcctgcgg ctcctgaccg
     2041 gcgccgaggt cgggaccgag ggtgagcacg gcgtggtcgt cgtcttcggc gatggccagc
     2101 tcgaccggcg cgcccgcgta gtggtgccag gcttcggcgg cgtcgacgcg gtgccagtgg
     2161 gagcgctcgt ggccgtcgag caggaagtag atggcgctgc ccacgccgcg cccgccatcg
     2221 gccggcaggt cccgccacgt ctcccggtac cacccgccct cggggtgcgg caccaatccc
     2281 agccgttcga tgacctcgcc tgcgtccatc gccccagtgt ggcgcggcgg ctcctcccgc
     2341 cggagcccgt gggagacgcc gcgaccggcc gcgctcgtcg cggtggaggg gtgggagctg
     2401 cggccgtcgg accgtgatcg cggtggcggc cgcaggttag gagctgaggg cgccgtctcg
     2461 gccggactgg ccggcggcct tccacctctc gc
//
LOCUS       DATMFS010000239         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_169890, whole genome shotgun sequence.
ACCESSION   DATMFS010000239 DATMFS010000000
VERSION     DATMFS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169890"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNQ33_04360"
     CDS             complement(<1..469)
                     /locus_tag="VNQ33_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWJ97369.1"
                     /translation="MAHTHSRVYRDGVVVERDIDDDRISDALAEPGTIVWVDLCDPTE
                     GDLDKLAFELGLHELAVEDALGDHQRPKLDRYRDHLFLTCHAAAVDTESAQLQETEIN
                     AFIGKEWLITVRKSRGFDICEVQDRWDRSPDLAVHGVSFLLYGLLDVVVDSYFD"
     gene            606..3452
                     /gene="uvrA"
                     /locus_tag="VNQ33_04365"
     CDS             606..3452
                     /gene="uvrA"
                     /locus_tag="VNQ33_04365"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWJ97370.1"
                     /translation="MSDSLIIRGAREHNLRNVSLDLPRDRLIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGQMDKPDVDFIEGLSPAISIDQKSASRNPRSTVGTV
                     TEIYDYLRLLFARIGVPHCPEHGVVISRQTPQQIVDRVLELPEGSRFQVLAPVVRGRK
                     GDYHTLLADLAAQGYARARIDGELHDLPVQDLELARYENHTIEVVVDRLVQREGIERR
                     LTDSLETALRLAEGVAEVELVPREEGEEPTRLTFSQHLACPVDGKSYDELSPRNFSFN
                     SPYGACPRCDGLGTQFEVDAELVVPDPDATLAEGAIQPWASGHMQYHQRLLESVCEDH
                     GIAFDKPWRTLTAKQQRMLLHGTGVGKTDVRYKNRYGRVRTYTATYEGAITWIQRRHA
                     DTDSDSQREQFEGYMREVDCPACEGARLKPFTLAVTIDGKSINEFSTISIKDAAEAFG
                     RIELSERDKLIAERVLKEINARIGFLLDVGLDYLSLHRSSATLAGGEAQRIRLASQIG
                     SGLVGVLYVLDEPSIGLHQRDNRRLIDTLTRLRDLGNTVLVVEHDEDTMAVADYVVDI
                     GPGAGEHGGAIVHAGTYKSLLKNKDSLTGQYLSGKRSIPVPATRRTPNGQKLAVKGAR
                     EHNLQGIDVDFPLGVLLAVTGVSGSGKSTLVNDILLRSLMQRIYKSKTPPGLHKRVTG
                     IEHLDKVINIDQSPIGRTPRSNPATYTGVWDHIRKLFAQTTEAKVRGYLPGRFSFNVK
                     GGRCENCAGDGTIKIEMHFLPDVYVPCEVCKGARYNRDTLDIEFKGKNVAEVLDLPCE
                     EALEFFANQPVIARHMQTLVDVGLGYVRLGQPAPTLSGGEAQRIKLASELSKRSTGHT
                     IYVLDEPTTGLHFEDIRKLLTVLGRLVDQGNTVLVIEHNLDVIKTADWIIDLGPEGGS
                     GGGTVLIEGTPETVAATPESYTGQFLRPLLGLEG"
BASE COUNT          605 a         1313 c         1195 g          516 t
ORIGIN      
        1 cgtcgaagta gctgtcgacc acgacgtcga gcaggccgta cagcaggaag ctcacgccgt
       61 ggaccgccag gtcgggggag cggtcccagc gatcctgcac ctcgcagatg tcgaagcccc
      121 tcgacttccg caccgtgatg agccactcct tcccgatgaa ggcgttgatc tcggtctcct
      181 gcagctgcgc cgactcggtg tcgacagctg ctgcgtggca cgtcaggaac aggtggtccc
      241 ggtagcggtc gagcttcggg cgctggtggt cgccgagggc gtcttcgacg gccagctcgt
      301 ggaggcccag ctcgaacgcc agcttgtcca agtcgccctc cgtcgggtcg cagaggtcga
      361 cccacacgat ggtgcccggc tcggcgaggg cgtcggagat gcggtcgtcg tcgatgtcgc
      421 gctcgacgac gacgccgtcg cggtacacgc gggagtgggt gtgggccatc ggctacgagc
      481 gtgccaggag cagagcgttc acgggcacga tcttgtcccg ccacccggtg gggagcggtg
      541 atctcggacc ggagcagaac aggtgttcgc caaacggccg gccggcgccg ataggctgcc
      601 gccctgtgtc tgactcgctg atcatccgcg gtgcccgcga gcacaacctc cgcaacgtct
      661 cgctcgatct gccccgcgac cggctcatcg tgttcaccgg gctgtccggg tcggggaagt
      721 cctccctggc cttcgacacc atctacgccg agggccagcg ccgctacgtc gagtcgctgt
      781 cgtcctacgc ccggcagttc ctcgggcaga tggacaagcc cgacgtcgac ttcatcgagg
      841 gcctgtcacc ggccatctcc atcgaccaga agtcggcgag ccgcaacccc cgctcgacgg
      901 tgggcaccgt caccgagatc tacgactacc tgcggctgct gttcgcccgc atcggcgtgc
      961 cgcactgtcc cgagcacggg gtggtgatca gccgccagac gccccagcag atcgtcgacc
     1021 gcgtgctcga gctgcccgag ggcagccggt tccaggtgct ggcccccgtg gtccgcggcc
     1081 gcaagggcga ctaccacacg ctgctcgccg acctggccgc ccagggctac gcccgggccc
     1141 gcatcgacgg cgagctgcac gacctgccgg tgcaggacct cgagctggcc cgctacgaga
     1201 accacaccat cgaggtcgtg gtggaccggc tggtgcagcg cgagggcatc gagcgccgcc
     1261 tcaccgactc gctggagacg gcgctgcgcc tggccgaggg cgtcgccgaa gtggagctgg
     1321 tgccgcgtga ggagggcgag gagcccaccc gcctgacctt cagccagcac ctcgcctgcc
     1381 cggtcgacgg caagagctac gacgagctgt cgccccggaa cttctcgttc aactcgccct
     1441 acggcgcctg cccgcgctgc gacggcttgg gcacccagtt cgaggtcgac gccgagctgg
     1501 tggtccccga ccccgacgcc acgctggccg agggcgctat ccagccctgg gcatcgggcc
     1561 acatgcagta ccaccagcgc ctgctggagt cggtgtgcga ggaccacggc atcgcgttcg
     1621 acaagccgtg gcgcacgctc accgccaagc agcagcggat gctcctgcac ggcaccggcg
     1681 tgggcaagac cgacgtgcgc tacaagaacc gctacgggcg ggtgcgcacg tacaccgcca
     1741 cctacgaggg cgccatcacc tggatccagc gccgccacgc cgacaccgac tccgacagcc
     1801 agcgcgagca gttcgagggc tacatgcgcg aggtcgactg cccggcctgc gagggtgccc
     1861 gcctcaagcc gttcaccctg gccgtgacca tcgacggcaa gagcatcaac gagttctcca
     1921 ccatctccat caaggatgcg gccgaggcgt tcgggcgcat cgagctgtcc gagcgcgaca
     1981 agctcatcgc cgagcgggtg ctcaaggaga tcaacgcccg catcgggttc ctgctcgacg
     2041 tcgggctcga ctacctgtcg ctgcaccgct cgtcggccac gctggctggc ggcgaggccc
     2101 agcgcatccg cctggccagc cagatcggct cgggcctcgt gggcgtcttg tacgtgctcg
     2161 acgagccgtc catcgggctg caccagcgcg acaaccggcg gctcatcgac acgctcacgc
     2221 gcctgcgcga cctcggcaac accgtgctgg tggtggagca cgacgaggac acgatggcgg
     2281 tggccgacta cgtggtcgac atcggccccg gagcggggga gcacggcggc gccatcgtcc
     2341 acgccggcac ctacaagtcc ctgctcaaga acaaggactc gctcaccggg cagtacctgt
     2401 cgggcaagag gtccatcccg gtgcccgcca cccggcggac gccgaacggc cagaagctgg
     2461 cggtcaaggg cgcccgcgag cacaacctcc agggcatcga cgtcgacttc ccgctcggcg
     2521 tgctgctcgc ggtcaccggc gtctccggct cgggcaagtc cacgctcgtc aacgacatcc
     2581 tgctgcgctc gctgatgcag aggatctaca agtcgaagac cccacccggc ctgcacaagc
     2641 gggtgacggg catcgagcac ctggacaagg tgatcaacat cgaccagtcg cccatcgggc
     2701 ggacaccccg gtcgaacccg gccacctaca ccggggtgtg ggaccacatc cggaagctct
     2761 tcgcccagac caccgaggcg aaggtgcgtg gctacctgcc cgggcgcttc agcttcaacg
     2821 tcaagggcgg ccgctgcgag aactgcgccg gcgacggcac catcaagatc gagatgcact
     2881 tcctgcccga cgtctacgtc ccgtgcgagg tgtgcaaggg ggcgcggtac aaccgggaca
     2941 cgctcgacat cgagttcaag ggcaagaacg tcgccgaggt gctggacctg ccgtgcgagg
     3001 aggcgctcga gttcttcgcc aaccagccgg tcatcgcccg ccacatgcag acgctggtcg
     3061 acgtcggcct cggctacgtg cgcctcgggc agccggcacc cacgctgtcg ggcggcgagg
     3121 cccagcgcat caagctggcg tcggagctgt cgaagcggtc caccggccac accatctacg
     3181 tgctcgacga gcccaccacc ggcctgcact tcgaagacat ccgcaagctc ctgaccgtgc
     3241 tcggccgcct ggtcgaccag ggcaacaccg tgctggtcat cgagcacaac ctcgacgtca
     3301 tcaagacggc cgactggatc atcgacctcg gtcccgaggg cggctccggc ggcggcaccg
     3361 tgctcatcga gggcacaccc gagacggtgg cggccacgcc ggagtcctac accggccagt
     3421 tcctccggcc cctcctcggc ctggaaggct gagcccatgg cgctcgacga cctcgacctg
     3481 cacgccctct accacctcgc caccgaagcg gagtgggagg agtaccagga ggagggtgcg
     3541 atcgtcccgc cgtcgctgga gaccgagggc ttcgtccacg gctcgtgggg ccaccaggtc
     3601 ccgggcacgg tgaccaagca cttcgccca
//
LOCUS       DATMFS010000240         4522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_54723, whole genome shotgun sequence.
ACCESSION   DATMFS010000240 DATMFS010000000
VERSION     DATMFS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54723"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VNQ33_04370"
     CDS             <1..365
                     /locus_tag="VNQ33_04370"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441992.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWJ97371.1"
                     /translation="IPGVVHGMHSYLMQDEWGQVSEAESISAGLDYPGVGPEHAHLSD
                     IGRAQYHPVTDAEVIEAFQALTRTEGIIPALEPAHGLAWILREAPSLQGQTVLLNLSG
                     RGDKDVAQMMDILGEDVS"
     gene            362..1171
                     /gene="trpA"
                     /locus_tag="VNQ33_04375"
     CDS             362..1171
                     /gene="trpA"
                     /locus_tag="VNQ33_04375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWJ97372.1"
                     /translation="MSPAAPLPAAPAPGPMETSLRARRDGGRKLLVPYLTGGLGPWTE
                     HLRAMADAGADVIEVGIPFSDPAMDGPVIQQASERALQQGTTPGSVLADLRGVDAGVP
                     LVTMTYYNLVFHMGDERMAGELADAGVSGVVLPDVPMEEQGPWRAAAEGAGIETVLMV
                     GPITPDDRLARVCEQSRGYIYGVNLMGITGERKSLGEEGAVLARRVKSLTDKPVLMGF
                     GVSTPEQAVEVSASSDGAIVGTAVVRRILAGESPEQLHAFIASLRKALDES"
     gene            complement(1335..1802)
                     /locus_tag="VNQ33_04380"
     CDS             complement(1335..1802)
                     /locus_tag="VNQ33_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97373.1"
                     /translation="MTFARPSSRAVAATLMAAAIGLLAPACSSGGSDAAKGTTTTAAP
                     TTTEAPTTTTAPATTTPAAPVGLPDQEAVAQKLYDAWKANDKVTAATVADPVAIDNIW
                     RAAPGDYSLYNSCSTGEFDTSGCLFRGNPGTIQIDLEKRGDNWVVAGAFYSEP"
     gene            complement(1841..2509)
                     /locus_tag="VNQ33_04385"
     CDS             complement(1841..2509)
                     /locus_tag="VNQ33_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ97374.1"
                     /translation="MSDESGPAGQAYVVAPDSGSGPGILVLHAWWGLTPFFHSVCDKL
                     ADAGFVALAPDLHGDGRTADTPDEAEALLASTDPNRTASLVVSSMSALRSMPATPDGP
                     IGILGYSMGASWAFWAATRFPDDIAAVTAYYGSQDIDFSPAKAAFQGHFAEIDEFESE
                     DDVNYLEAQLKLCGHEVEFHRYPGTGHWFAEADRAAAHVPDADERAWARTVEFLHRRL
                     DSRR"
     gene            2570..2899
                     /locus_tag="VNQ33_04390"
     CDS             2570..2899
                     /locus_tag="VNQ33_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97375.1"
                     /translation="MTEDRAFDDGCPLCEAARITPWHHEDDVCWIADCEICATPMVVW
                     RGHGTEPPEHERGHMMAQLVRVAQANLGAHWIDGSMRNIPNHFHAHARPDGGFFGPGG
                     GPGRLAR"
     gene            3066..3473
                     /locus_tag="VNQ33_04395"
     CDS             3066..3473
                     /locus_tag="VNQ33_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HWJ97376.1"
                     /translation="MPVDGPDVNVFEAVGGLSWFVALVERFYAGVADDPILRPMYPED
                     LSGPIEHTAGFLAQYWGGGTAMYSDHRGHPRLRMRHAPFVIGQAERDAWLGHMVDAVK
                     GGGLPPAIEQRMIDYFETASTHMINDSPLGLRP"
     gene            3491..3955
                     /gene="bcp"
                     /locus_tag="VNQ33_04400"
     CDS             3491..3955
                     /gene="bcp"
                     /locus_tag="VNQ33_04400"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWJ97377.1"
                     /translation="MADAPAAGDRAPAFNLNDQNGAKLRLSSFKGRKVLVYFYPKADT
                     PGCTTQSCELRDIKDDIGDTAVVGISPDEPEKLKKFDEKYGLGFTLLSDPDHVTAEKY
                     GVWGEKVNYGRKYMGIIRSAFLIDEKGKVSHSWPKISPKDTPKKLLKALAEG"
     gene            4036..4119
                     /locus_tag="VNQ33_04405"
     tRNA            4036..4119
                     /locus_tag="VNQ33_04405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4070..4072,aa:Leu,seq:caa)
     gene            complement(4165..>4522)
                     /locus_tag="VNQ33_04410"
     CDS             complement(4165..>4522)
                     /locus_tag="VNQ33_04410"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWJ97378.1"
                     /translation="VWSGADLADQRAAGVTVPADGDELFRHRLRVAASCDDGADVDDV
                     AVAAHAALAAAPSMLVTAALDDLVGAEHRPNVPGTIDEHPNWRIPLPVPLDDLAATPL
                     ADRIVAAISTGRGHQG"
BASE COUNT          637 a         1563 c         1672 g          650 t
ORIGIN      
        1 gcatccccgg cgtggtccac gggatgcact cgtacctcat gcaagacgag tgggggcagg
       61 tctccgaggc cgagtccatc tccgccggtc tcgactaccc gggcgtcggc cccgagcacg
      121 cccacctgtc cgacatcggc cgggcgcagt accacccggt caccgacgcc gaggtcatcg
      181 aggcgttcca ggccctcacc cgcaccgagg gcatcatccc ggcgctcgaa ccagcccacg
      241 ggctggcctg gatcctccgc gaggccccga gcctgcaggg gcagaccgtc ctgctcaact
      301 tgtcgggccg gggagacaag gacgtcgccc agatgatgga catcctcggg gaggacgtgt
      361 cgtgagccct gccgccccgc ttcccgctgc cccggccccc ggcccgatgg agacgtcgct
      421 gcgcgcccgg cgcgacggcg gccgcaagct cctcgtcccg tacctcacgg gcggcctcgg
      481 gccctggacc gagcacctcc gcgccatggc cgacgccggc gccgatgtca tcgaggtcgg
      541 catcccgttc tccgatccgg ccatggacgg cccggtcatc cagcaggcat cggagcgggc
      601 gctccagcag ggcaccacgc ccggctcggt cctcgccgac ctgcgcggcg tcgatgccgg
      661 cgtcccgctg gtgacgatga cctactacaa cctcgtgttc cacatgggcg acgagcgcat
      721 ggccggcgag ctggccgatg ccggggtgtc cggcgtcgtg ctgcccgacg tgccgatgga
      781 ggagcagggc ccgtggcgag ccgcggccga aggcgccggc atcgagacgg tgctgatggt
      841 cgggccgatc acccccgacg accgcctcgc ccgcgtctgc gagcagagcc ggggctacat
      901 ctacggcgtc aacctcatgg gcatcaccgg cgagcggaag tcgctcggcg aggagggtgc
      961 cgtcctcgcc cgccgggtga agtccctcac cgacaagccg gtcctgatgg gcttcggcgt
     1021 cagcacgccc gagcaggccg tcgaggtgtc ggcctcttcc gacggcgcca tcgtcggcac
     1081 cgccgtcgtc cgccgcatcc tggccggcga gagccccgag cagctccacg ccttcatcgc
     1141 cagcctcagg aaggccctcg acgagtcctg agcacccccc atcccgtccg aacttcgtag
     1201 gtcagggcga ccgtttggtt gcgctcccct acgaagttcg ccgggtctcc gggtcctcgc
     1261 ctgaacttcg taggccaggg cgacgatgtc gtcgtgctcg cctacgaagt tcgttggagg
     1321 ttcggaccgg agggtcaggg ttcgctgtag aaggcgccgg cgacgaccca gttgtcgccg
     1381 cgcttttcga ggtcgatctg gatggtgccg gggttgccgc ggaagaggca gcccgaggtg
     1441 tcgaactcgc cggtggagca gctgttgtag agcgagtagt cgcccggtgc ggcgcgccag
     1501 atgttgtcga tggcgaccgg gtcggcgacg gtggccgccg tgaccttgtc gttcgccttc
     1561 caggcgtcgt agagcttctg ggccaccgct tcttggtcgg gcaggcccac cggtgcggcg
     1621 ggtgtcgtgg tggcgggggc cgtggtggtg gtcggcgctt ccgtcgtggt gggtgccgcg
     1681 gtggtcgtgg tgcccttggc ggcgtcgctg ccaccggagg agcaggccgg ggcgaggagg
     1741 ccgatggcag cggccatcag cgtggcggcc acagcgcggg agctcggtcg tgcgaacgtc
     1801 atggtcggcc accgtaacgg ccgcccgccc ggcccccggg tcagcgtcgg ctgtcgaggc
     1861 gacggtggag gaactcgacc gtgcgcgccc aggcccgctc gtcggcgtcg ggcacgtggg
     1921 cggcggcgcg gtcggcctcg gcgaaccagt ggccggtgcc ggggtagcgg tggaactcga
     1981 cctcgtggcc gcagagcttc agctgggctt cgaggtagtt gacgtcgtct tcggattcga
     2041 actcgtcgat ctcggcgaag tgcccctgga acgcggcctt ggcgggcgag aagtcgatgt
     2101 cctggctgcc gtagtacgcg gtgacggcgg cgatgtcgtc ggggaagcgg gtggcggccc
     2161 agaacgccca cgacgcgccc atcgagtagc cgaggatgcc gatggggccg tcgggcgtgg
     2221 ccggcatcga gcggagggcc gacatcgacg acaccaccag gctggccgtg cggttcgggt
     2281 cggtcgacgc gagcagcgcc tcggcctcgt cgggcgtgtc ggccgtccgc ccgtcgccgt
     2341 ggaggtccgg ggcaagcgcc acgaacccgg cgtcggccag cttgtcgcac accgagtgga
     2401 agaacggggt gagcccccac cacgcgtgca gcaccaagat cccggggccc gaccccgagt
     2461 ccggagccac cacgtacgcc tggccggcag gaccggattc gtcggacatg gcgtcgaccg
     2521 tagcggtgtc gtcctcgtgc acccgcgtgc cgcccgtacg atgggcgcgg tgacggagga
     2581 tcgtgcgttc gacgacgggt gcccgctgtg cgaggcggcg cggatcacgc cgtggcacca
     2641 cgaggacgac gtgtgctgga tcgccgactg cgagatctgc gccacgccca tggtcgtgtg
     2701 gcggggacac ggcaccgagc cgccggagca cgagcgcggc cacatgatgg cgcagctcgt
     2761 gcgggtggcc caggccaacc tgggcgccca ctggatcgac ggcagcatgc gcaacatccc
     2821 gaaccacttc cacgcccacg cccgtcccga tggcgggttc ttcgggcccg gcggcggccc
     2881 cgggaggctg gcccgatgat cgagcggatg caggccggca cccgctcggc tgggctccgg
     2941 cagcatcggc aggggcgcgg cagccgtcgt ggcggggtgc gccgtcgtgc tcctcgccgt
     3001 ggcgatggtc ctctgctcga cgggtcgccg cccggtcccg cagcgcggcg gccgacgtgg
     3061 tgaccatgcc ggtcgacggg ccggacgtga acgtgttcga ggcggtgggc gggctgtcgt
     3121 ggttcgtcgc cctggtggag cggttctacg ccggggtggc cgacgacccg atcctgcggc
     3181 cgatgtaccc cgaggacctc agcggtccca tcgagcacac cgcgggcttc ctcgcccagt
     3241 actggggcgg cggcaccgcg atgtactccg atcaccgcgg ccacccgcgc ctgcggatgc
     3301 gccacgcgcc gttcgtgatc ggccaggccg agcgcgacgc ctggctcggc cacatggtcg
     3361 atgccgtgaa gggcggtggg ctgccgccgg cgatcgagca gcggatgatc gactacttcg
     3421 agacggcgag cacccacatg atcaacgaca gcccgctcgg cctccgcccc tgagccggta
     3481 cggtgagcac atggctgatg ctcctgctgc tggtgaccgc gctcccgcct tcaacctcaa
     3541 cgaccagaac ggggccaagc tccggctgtc gtcgttcaag gggcgcaagg tgctcgtgta
     3601 cttctacccg aaggccgata cgcctggctg caccacgcag tcgtgcgagc tgcgcgacat
     3661 caaagacgac atcggcgaca cggccgtcgt cggcatcagc cccgatgagc ctgagaagct
     3721 gaagaagttc gacgagaagt acggcctcgg cttcaccctc ttgtccgacc ccgatcacgt
     3781 cacggccgag aagtacggcg tctggggcga gaaggtcaac tacggccgca agtacatggg
     3841 catcatccgc tcggcgttcc tcatcgacga gaagggcaag gtcagccact cgtggccgaa
     3901 gatcagcccg aaggacacgc cgaagaagct gctgaaggcc ctcgccgaag gctgaccggc
     3961 cggcggacgc cgcgccgatc ggctgcgggt ctacggagga gcgacgccta cactcggcgc
     4021 tcgcagcacc accttgcccg ggtggcggaa ttggcagacg cgggggactc aaaatcctct
     4081 gccctcacgg gcgtgcgggt tcgattcccg ccccgggcac tacggcacct cgcagcggcc
     4141 gccggagcgg cacggcaggg ggacctaccc ctggtggccg cggccggtgc tgatggcggc
     4201 gacgatgcgg tcggcgaggg gcgtggccgc caggtcgtcg agtgggacgg ggagggggat
     4261 gcgccagttg gggtgctcgt cgatggtgcc ggggacgttg gggcggtgct cggctcccac
     4321 cagatcgtcg agggcggcgg tcacgagcat cgacggcgcc gcggcgaggg cggcatgggc
     4381 ggcgacggcc acgtcgtcca cgtcggcgcc gtcgtcgcac gacgcggcga cgcggaggcg
     4441 gtggcggaac agctcgtcgc cgtcggcggg caccgtgacc ccggcggccc gctggtcggc
     4501 taggtccgcg ccggaccaca cg
//
LOCUS       DATMFS010000241         7663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_32386, whole genome shotgun sequence.
ACCESSION   DATMFS010000241 DATMFS010000000
VERSION     DATMFS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32386"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1100
                     /locus_tag="VNQ33_04415"
     CDS             189..1100
                     /locus_tag="VNQ33_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWJ97379.1"
                     /translation="MRKVIVLAIVGFFAQLIDGALGMAYGVTSSSLMLAAGLAPAAAS
                     ASVHLAEIGTTLASGASHWRFGNVDWKIVLRLGVPGAIGAFAGATFLSGLSSDAAKPW
                     MAGILLALGVYVLLRFTFGSLKTPDGKTSLRARFLSPLGLIAGFIDATGGGGWGPVAT
                     PTLLVSGKVEPRKVIGSVDTSEFLVAVAASVGFFIGIGNEGVKLGYVVALLAGGLLAA
                     PIAAYLVRVIPPQLLGSLVGGFIVLTNSRTILNASDVTGGAAAAVYLSVTVLWAAAVA
                     WSVKRYRAAKAADVEGARSSAPSIAPA"
     gene            complement(1225..1593)
                     /locus_tag="VNQ33_04420"
     CDS             complement(1225..1593)
                     /locus_tag="VNQ33_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97380.1"
                     /translation="MKDRTLVSSIAAPLVVAVLLSSWLLWTRSAPHRQGGYCMNASVE
                     IAGVLQRADTSGDVGRGPLPPVGKILAEVDQVDVTRFQVNTPPEVQDDVDLLVAERDP
                     AAFARIAQDYLERCRKASTE"
     gene            complement(1590..3227)
                     /locus_tag="VNQ33_04425"
     CDS             complement(1590..3227)
                     /locus_tag="VNQ33_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97381.1"
                     /translation="MAEAMKRTGTRVRDLAPRDRALALVLIIALGAAPFAAAVSGLAK
                     GWQPTGDVAVISLRTLDVWKGHPPLLGQTSTAEELTGTPANHPGPIENWVLAGTTKAA
                     GDRAGIVVGTALINSAALVGVAWLCVRRGGVALLALMSVAVAGLVWSLGAASLYDPFN
                     SELATYPMLVAIVAAWCVLVGDLRVTPILAAAVTVVGQLHIVGPGFCAPLVLIALAAI
                     GLAWRRHPGTVRRDRAYLFGALGVLAVGWSPVLAYELGPEPSNVAALWKAATTPRPRI
                     GLSFTLERLATAVAPVPTFLRRTGRFGFISDVSPVQVFLAFLIIAGGVALSPLVKRRD
                     GRNHPGRLAWLLVAVAVTSTISGSGQPPLSAFRADGTRWLWVVSLGFWVALAWEGWNL
                     VSDARRDALRRPLAIGGGAVAVLVLLGALGTARLADQRDGDLMSVTDRTASAVIDDLP
                     EGTYHVRFEGNQALVAVGPGVVYRLEASGSDVVLDRNVFTRGYTDRRTDGRTTDAQLR
                     ITSDAGAKANDGEQLLSSEAVDPGDPDAGTIKVFVTR"
     gene            complement(3237..3830)
                     /gene="leuD"
                     /locus_tag="VNQ33_04430"
     CDS             complement(3237..3830)
                     /gene="leuD"
                     /locus_tag="VNQ33_04430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWJ97382.1"
                     /translation="MDAVRIVTGTAVPLDRSDVDTDQIIPSDWLKQVERTGFEKGLFS
                     EWRDDRDFVLNQEQYAGATILIGGPNFGTGSSREHAVWAIQQYGFRAVVSPRFGDIFR
                     NNAGKNGLLPVVVPPEVGEQLMRAVEADPTLEITIDVERRTVEAPAIGLTVEFPLDPS
                     VQHRILEGLDDIGITLRQADDITAYETSRPDWLPTTS"
     gene            complement(3834..5237)
                     /gene="leuC"
                     /locus_tag="VNQ33_04435"
     CDS             complement(3834..5237)
                     /gene="leuC"
                     /locus_tag="VNQ33_04435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWJ97383.1"
                     /translation="MTAPKTLSQKVWDAHVVSQTPGEPDLLFIDLHLVHEVTSPQAFD
                     GLRLAGRGVRHPELTVATEDHNVPTENTNLEIADPISRKQVQTLRNNCAEFGITLHPM
                     NTPGQGIVHIIGPEQGLTLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
                     PQAAAKTLAITVDGDLPAGSTAKDVILAILGRIGTGGGIGHIAEYRGSAIRALSMEGR
                     MTVCNMSIEAGAKAGLIAPDQVTFDYLEGRDHAPKGAAWDEAVAYWKTLPTDEGAAFD
                     KEVVLDAAEIFPHVSWGTNPGQVAPITDRVPDPASFQDPTERNAAERALEYMGLTAGQ
                     PLKEVAVDTVFIGSCTNGRIEDLRAVAEVAKGRTVSSGMRTLVVPGSWRVKAQAEAEG
                     IDQVLTEAGFDWRDPGCSMCLAMNPDKLAPGERSASTSNRNFEGRQGRGGRTHLVSPA
                     VAAATAIAGHFATPEDL"
     gene            5306..5968
                     /locus_tag="VNQ33_04440"
     CDS             5306..5968
                     /locus_tag="VNQ33_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWJ97384.1"
                     /translation="MEQTLSGVGVVDKSVRVLDAVAESPGSCTLGELVDATGIPKATA
                     HRLAAALEVHGLLRRDRDGRYLLGVRLIGLGRAAAEDWPLAEAARPALEALRDTTGES
                     VQLYRRDGDHRVCVVSLESPHELRTIVPQGSRLPLGLGSAGRILSGEAAGEPWASSVG
                     ERAPGVASVSAPVVVDGRLVAAVGISGPVGRLGDHPGDRHGAAVTEAAAAIGAASARA
                     GA"
     gene            6110..>7663
                     /locus_tag="VNQ33_04445"
     CDS             6110..>7663
                     /locus_tag="VNQ33_04445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ97385.1"
                     /translation="MQLNPAKFARPVHSDGRRYVPAIDGLRVLAAFGVISAHVGSEYG
                     PSQHLVAYYGAAAYPALIVFFTISGFLVYRPFARAALAPRVKKASDRSTNFWVYILRR
                     VLRIFPLYWAVLFVAVLWNGAGNLHSLADWAQAVFLLPFPDPMKLLSGPLGIAMWTLA
                     IELPFYVVVPLFSAGVKQARIRLAPESDPFYAQVLATCSLIALFFILGLIFGGELAFP
                     VMSLPVGMLLAVLETRQALARRRYSGIKFLADNWWLCLVGYVLLVLVGAGLALDAYSS
                     QEALRAAAEGRGFDPYAHIRNLYFPLQLAMALLLFIPVAFGQRKSLMVRFMAAAPLQI
                     IAPLTYGIYLWHVPLIRHTSEWFGPGFLGFPVPGTTGFGMPTLFLAIGLAILTFVFVE
                     LPAAKLRDAVDRWSRGQGRVAGEPTDEPEGEESSEFGGGADRVVDRIPDHMWRPDKKA
                     PVYVRNVGEGDDEYSDAEAPLDDVVVDAGHPGADPSRQPVGAGAPAGPSRRPNSARAM
                     SRAGEDANPQ"
BASE COUNT          973 a         2659 c         2866 g         1165 t
ORIGIN      
        1 cgcagcttct tcagcaggag cccggctagg accccgaggc cgaggaggac cgccagcttg
       61 cgcaccttgc cttcctgcat ggctccgcac cctacaagcg ttcatcgaca ccgccgcgcc
      121 gagcgccgag gttgcgcgcc acggaaccat gtccgtaata tctaccggac cgctaggaaa
      181 ggtccgcaat gcgcaaggtg atcgtgctcg caatcgtcgg gttcttcgcc cagttgatcg
      241 acggcgcgct gggcatggcg tacggcgtga cctcgtcgtc gctcatgctc gcggccggcc
      301 tcgctccggc tgcggcatcg gcctcggtgc acctcgcgga gatcggcacc acgctggcgt
      361 cgggggcatc gcactggcgg ttcggcaacg tcgactggaa gatcgtgctg cgactcggcg
      421 tgccgggcgc catcggggcg ttcgccggcg ccaccttcct gtccggcctc tccagcgatg
      481 ccgccaagcc gtggatggcg ggcatcctgc tcgccctcgg cgtgtacgtc ttgttgcgct
      541 tcaccttcgg ctcgctcaag acacccgatg ggaagacctc gctgcgggcc cgcttcctca
      601 gcccgctcgg tctcatcgcc gggttcatcg acgccaccgg tggcggtgga tggggtcccg
      661 tggccacgcc cacgctgctg gtctcgggca aggtcgagcc ccggaaggtg atcggctcgg
      721 tcgacacgag cgagttcctg gtggccgtcg ccgcgagcgt cggcttcttc atcgggatcg
      781 gcaacgaggg cgtgaagctc gggtacgtgg tggccctgct ggccggcggg ttgctcgccg
      841 cgcccatcgc cgcctacctc gtccgggtca tcccgccgca gctcctcggc tcgctcgtcg
      901 gcggcttcat cgtgttgacc aactcccgca ccatcctcaa cgcgtccgac gtcaccggtg
      961 gtgccgcggc ggcggtgtac ctgtcggtca cggtcctgtg ggcggcagcc gtcgcctggt
     1021 cggtgaagcg ctaccgcgcc gccaaggccg ccgacgtcga gggcgcccgc tcgtccgcac
     1081 cgtccatcgc ccccgcctga tcggccgggc cagcccgtgc tcttgcggag cgggccgcga
     1141 ggtgggcccg gcgctccagg cgggcggtga ccgtgaagcc gccgcagacg tgcccggtgg
     1201 tggacgcgcc caccaccgac ggggtcactc ggtggaggcc ttgcggcagc gctcgaggta
     1261 gtcctgggcg atgcgggcga acgccgccgg gtctcgctcc gccaccagca ggtcgacgtc
     1321 gtcttggacc tcgggcgggg tgttgacctg gaagcgggtg acgtcgacct ggtcgacctc
     1381 ggcgaggatc ttgccgaccg gtgggagcgg cccgcggccg acgtcgccgc tggtgtcggc
     1441 ccgctggagg acgccggcga tctccacgct ggcgttcatg cagtagccgc cctggcgatg
     1501 gggggccgag cgcgtccaga ggagccagga cgacagcagc acggcgacca cgaggggagc
     1561 ggcgatgctc gagacgagcg tgcggtcctt caccgggtca cgaacacctt gatcgtgccg
     1621 gcgtcgggat cgccggggtc gacggcctcg gacgagagga gctgctcgcc gtcgttcgcc
     1681 ttggctccgg cgtctgaggt gatgcgcagc tgggcgtcgg tggtgcgccc gtcggtgcgg
     1741 cgatcggtgt agccccgggt gaagacgttc cggtcgagga cgacgtcgga cccggacgcc
     1801 tccaggcggt agacgacgcc ggggcccacg gcgaccaggg cctggttgcc ctcgaagcgc
     1861 acgtggtacg tgccttcggg caggtcgtcg atcacggcgg acgcggtccg gtcggtgacc
     1921 gacatgaggt cgccgtcgcg ctggtcggcg agcctggccg tgcccagggc gccgaggagc
     1981 acgagcacgg ccacggcacc gccgccgatg gccagcggcc ggcgcagcgc gtcccgccga
     2041 gcatcggaga ccaggttcca cccctcccag gcgagcgcca cccagaagcc gaggctgacg
     2101 acccacagcc agcgggtgcc gtcggcgcgg aacgccgaga gcggtggctg gcccgagccg
     2161 ctgatggtgg acgtgacggc cacggccacg agcagccagg cgagacggcc ggggtggttg
     2221 cggccgtcac ggcgcttcac caggggcgag agggcgacgc cgcccgcgat gatcaagaag
     2281 gccaggaaga cctggacggg gctgacgtcg gagatgaagc cgaaccgccc ggtgcggcgc
     2341 aggaacgtgg ggaccggggc cacggccgtc gccaggcgct cgagggtgaa cgagaggccg
     2401 atgcgcgggc ggggggtcgt ggctgccttc cacagcgcgg ccacgttgct cggctccggg
     2461 ccgagctcgt aggcgaggac cggcgaccag ccgacggcga gcacgccgag agcaccgaac
     2521 aggtaggccc ggtcccgccg caccgtgccc gggtggcggc gccacgccag gccgatggcg
     2581 gcgagggcga tcagcacgag cggcgcgcag aaccccggtc cgacgatgtg gagctggccg
     2641 accacggtga cggcggcagc gaggatcggg gtgacccgga ggtcgcccac gagcacgcac
     2701 caggccgcca cgatggcgac gagcatcgga taggtggcca gctccgagtt gaacgggtcg
     2761 tacagggagg cggcgccgag cgaccagacg aggccggcca ccgccaccga catcagggcc
     2821 aggagggcca cgccgccgcg tcgcacgcac agccacgcca cgcccaccag ggcggcgctg
     2881 ttgatcagcg cggtgccgac cacgatgccg gcccggtcgc ccgccgcctt cgtggtcccg
     2941 gcgagcaccc agttctcgat cggacccggg tggttcgccg gggtgccggt cagctcctcg
     3001 gcggtgctgg tctggccgag gaggggcggg tggcccttcc acacgtcgag ggtccgcagc
     3061 gagatgaccg ccacgtcgcc ggtgggctgc cagcccttcg ccagccccga gacggcggcg
     3121 gcgaacgggg cggcgccgag ggcgatgatc aacacgagcg cgagggcccg gtcccgaggt
     3181 gcgaggtccc ggacgcgcgt gcccgttcgc ttcatcgcct cggccatcgg catggtctag
     3241 ctggtggtcg gcagccaatc gggacgggag gtctcgtagg cggtgatgtc gtcggcctgg
     3301 cgcagcgtga tgccgatgtc gtcgagccct tcgaggatcc ggtgctggac cgaggggtcc
     3361 agggggaact cgacggtgag cccgatggcg ggcgcctcga ccgtgcggcg ctccacatcg
     3421 atcgtgatct cgagcgtcgg atcagcctcg acggcccgca tcagctgctc gccgacctcg
     3481 ggcggcacca cgacgggcag caggccgttc ttgccggcgt tgttgcggaa gatgtcgccg
     3541 aagcggggcg agacgacggc ccggaagccg tactgctgga tggcccacac ggcgtgctcg
     3601 cgggaggagc cggtgccgaa gttggggccg ccgatgagga tggtggcgcc ggcgtactgc
     3661 tcctggttga gcacgaagtc ccggtcgtcg cgccactccg agaagagccc cttctcgaac
     3721 ccggtgcgct cgacctgctt gagccagtcg gacgggatga tctgatcggt gtccacatcg
     3781 gaccgatcga gcgggaccgc ggtccccgtg acgatgcgta ctgcgtccat ggttcagagg
     3841 tcctcgggag tggcgaagtg gccggcgatg gccgtggcgg cggcgacggc gggggagacg
     3901 aggtgggtgc ggccgccccg tccctgccgg ccttcgaagt tgcggttgct ggtggaggcc
     3961 gagcgctcgc cgggggcgag cttgtcgggg ttcatggcca ggcacatcga gcagcccggg
     4021 tcgcgccagt cgaagccggc ttcggtgagg acctggtcga tgccctcggc ctcggcctgg
     4081 gccttgaccc gccacgagcc cgggaccacg agggtgcgca tgcccgagga gaccgtgcgg
     4141 cccttggcca cctcggccac cgcccgcagg tcctcgatgc ggccgttggt gcacgatccg
     4201 atgaacacgg tgtcgacggc cacctccttg aggggctggc cggcggtgag gcccatgtac
     4261 tccagcgccc gctcggcggc gttgcgctcg gtggggtcct ggaagctggc cggatccggc
     4321 acgcggtcgg tgatcggggc gacctggccg gggttcgtgc cccaggagac gtgcgggaag
     4381 atctcggcgg cgtcgagcac gacctccttg tcgaaggcgg cgccctcgtc ggtgggaagg
     4441 gtcttccagt aggcgacggc ctcgtcccac gccgcgccct tcggcgcgtg gtcccggccc
     4501 tcgaggtagt cgaaggtgac ctggtccggg gcgatgagcc cggccttggc cccggcctcg
     4561 atcgacatgt tgcagaccgt catccggcct tccatggaga gggcccggat ggccgagccg
     4621 cggtactcgg cgatgtggcc gatgccgccg ccggtgccga tgcgcccgag gatggcgagg
     4681 atgacgtcct tggcggtgct gccggcgggc aggtcgccgt cgacggtgat ggcgagggtc
     4741 ttggcggcgg cctggggcag cgtctgggtg gcgagcacgt gctcgacctc gctggtgccg
     4801 atgccgaacg ccagggcgcc gaacgcgccg tgggtcgagg tgtgcgagtc gccgcagacc
     4861 acggtcatgc cggggagggt gaggccctgc tccgggccga tgatgtggac gatgccctgg
     4921 cccggggtgt tcatcgggtg cagggtgatg ccgaactcgg cgcagttgtt gcgcagcgtc
     4981 tggacctgct tgcgggagat cgggtcggcg atctcgaggt tggtgttctc ggtcgggacg
     5041 ttgtggtcct cggtggcgac ggtcagctcc gggtggcgga cgccgcggcc ggccaggcgg
     5101 aggccgtcga aggcctgggg gctggtgacc tcgtgcacga ggtgcaggtc gatgaagagc
     5161 aggtcgggct ctccgggcgt ctgggacacc acatgggcgt cccagacctt ctggctcagg
     5221 gtcttgggtg cagtcatcgt gttcgctcgg ttcgcttcgg ctccgacttg cgttgtctca
     5281 atatatggga caacaatgtc aactgatgga acagacccta agcggtgtcg gcgtggtcga
     5341 caagtcggtc cgcgtgctcg acgccgtggc cgagtccccc ggctcgtgca cgctcggcga
     5401 gctggtcgac gccaccggca tcccgaaggc gactgcgcac cgcctcgccg ccgccctgga
     5461 ggtgcacggc ctcctgcggc gcgaccggga cggccgctac ctgctcggcg tccggctgat
     5521 cgggctgggc cgggcggcgg ccgaggactg gcccctggcg gaggcggccc ggccggcgct
     5581 ggaggcgctg agggacacga cgggggagag cgtgcagctc taccgccgcg atggcgacca
     5641 ccgcgtgtgc gtggtgtcgc tcgaatcgcc ccacgagctg cgcacgatcg tcccccaggg
     5701 ctcccggctg ccgctcggac tcgggtccgc cgggcgcatc ttgtcgggtg aggccgcggg
     5761 ggagccgtgg gcgtccagcg tcggcgagcg ggcgccgggc gtggcatcgg tgagcgcccc
     5821 ggtcgtggtc gacgggcgcc tggtcgccgc ggtcggcatc tcgggacccg tcggccgcct
     5881 cggtgaccac cccggagacc gccacggtgc cgcggtgacc gaagcggcgg cggcgatcgg
     5941 tgcggcttcg gcacgcgccg gtgcctgagc ccgtggcccc ggcgaccgcc cgaggtcggg
     6001 ctgcgggtgg tcggcggccg ggctggcaga ctggctcggc gatgctcctc acgtccctgg
     6061 tctccggagc cggcgcggcc ggcggccccc tgctcccgac ggagcgtccg tgcagctgaa
     6121 cccggcgaag ttcgcccggc ccgtccactc cgacggacgc cgctacgtcc cggccatcga
     6181 cggcctccgg gtgctggccg ccttcggcgt gatctcggcg cacgtcggct cggagtacgg
     6241 gccgtcgcag cacctggtgg cctactacgg cgctgctgcc taccccgcgc tgatcgtctt
     6301 cttcacgatc tcgggcttcc tcgtgtaccg gccgttcgct cgggccgcgc tggcaccccg
     6361 ggtcaagaag gcgtcggacc gcagcaccaa cttctgggtg tacatcctgc gccgggtcct
     6421 gcggatcttc ccgctgtact gggcggtgct gttcgtggcc gtgctgtgga acggcgccgg
     6481 caacctgcac tcgctggccg actgggcgca ggccgtcttc ctgctgccgt tcccggaccc
     6541 gatgaagctg ctgtcgggcc cgctcggcat cgccatgtgg acgctggcca tcgagctgcc
     6601 cttctacgtc gtcgtcccgc tcttctcggc gggggtcaag caggctcgga tccgcctggc
     6661 tcccgagagc gacccgttct acgcgcaggt gctggccacc tgctcgctca tcgcgctctt
     6721 cttcatcctc gggctcatct tcggcggcga gctggcgttc ccggtgatga gcctcccggt
     6781 cggcatgctc ctcgccgtcc tcgagacccg ccaggcgctc gcccgccgcc gctactcggg
     6841 catcaagttc ctggccgaca actggtggct gtgcctggtc gggtacgtgc tcttggtgtt
     6901 ggtgggtgcc gggctcgccc tcgacgccta ctcctcgcag gaggcgctgc gggctgcagc
     6961 cgagggacgt gggttcgacc cctacgccca catccgcaac ctgtacttcc cgctgcagct
     7021 ggccatggcg ctgctgctgt tcatcccggt cgccttcggc cagcgcaagt cgctcatggt
     7081 ccggttcatg gcggcggctc ccctgcagat catcgccccg ctcacttacg gcatctacct
     7141 ctggcacgtg ccgctgatcc ggcacacgtc cgagtggttc ggccccgggt tcctcgggtt
     7201 ccccgtgccg ggcaccaccg ggttcggcat gccgacgctg ttcctcgcca tcggcttggc
     7261 gatcctcacg ttcgtgttcg tcgagctccc ggccgccaag ctgcgcgacg ccgtcgaccg
     7321 ctggagccgg ggccaaggcc gtgtcgccgg cgagcccacc gacgagcccg agggcgagga
     7381 gtcgagcgag ttcggcggtg gcgccgaccg ggtggtcgac cgcatccccg accacatgtg
     7441 gcggcccgac aagaaggctc cggtctacgt ccgcaacgtc ggcgagggcg acgacgagta
     7501 ctccgatgcc gaagccccgc tcgacgacgt ggtcgtcgac gccggccacc cgggcgccga
     7561 tccctcgcgc cagcccgtcg gtgccggcgc gcccgcgggc ccgtcgcgcc gccccaactc
     7621 cgcccgggcc atgagccggg cgggcgagga cgccaacccc cag
//
LOCUS       DATMFS010000242         4514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_240293, whole genome shotgun sequence.
ACCESSION   DATMFS010000242 DATMFS010000000
VERSION     DATMFS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240293"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VNQ33_04450"
     CDS             <1..387
                     /locus_tag="VNQ33_04450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97386.1"
                     /translation="VPGEIDLLYIDGAHRFKPALDDIKRWSAKVRPGGDLLIHDSFSS
                     VGVTGAIGVSLLANPGWRYLGRSESMTHYRREPLTSNERVRNIGRQLASLPYFAKNLV
                     IKALILAKLGFLTQPLFQHDPATWPH"
     gene            375..1094
                     /locus_tag="VNQ33_04455"
     CDS             375..1094
                     /locus_tag="VNQ33_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ97387.1"
                     /translation="MAALMKAAPMTSLTIVMPVYNERETLRTALDRLLAVTMPVETEV
                     VVVDDGSTDGCTETITDLVESGAVTLYVQNPNQGKGAALRRGIAEAEGDLLTILDADL
                     EYDPSDFPALLQPILDGDARVVYGARSYGGHAAYSFWFVLGNKMLALWASFLFDAWLT
                     DIETCLKVAPTGHWRKVDLRSNGFGIEAELTGKLLTMNERIFEVPISYRARGREEGKK
                     IQWTDGVAALWILLRIRLRGT"
     gene            1091..2518
                     /locus_tag="VNQ33_04460"
     CDS             1091..2518
                     /locus_tag="VNQ33_04460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ97388.1"
                     /translation="MTAEVQPAPRRIIPPARTAALLGLLVLVGFVIRIDALRNSAPDV
                     PVLGDARAYHLLAENLADGRGYIRPYEFAADGTEIKTAEYPPALPVVLAAATKAGASS
                     ETSQRLLLCGVGALTVGLVGLIGRRLGGDSAGYLAALLAAIHPGLWNSDVSLMAEPLA
                     SFTGAALVVAALWVVNRPDRWRWAAFGAVAGLGCFVRSEFLLMGPALMAVVAWKTAEA
                     WRPRLGRLALGVGVLVAVLVPWTVRNLVAFDGQLVPLSNNSGSVAQGANCGAAYSGPF
                     KGLWVTNVSLDGNDADQSRAGCFAGFDLTDDDEAQAAARLRADGIGYLTDHKGQVPGV
                     VAARLGRTFGLYQFEQQRNFAGAEGRNVLWDGRGTRSFQLLALVGIAGLVVSSVRRTR
                     TWERWLLVVPPAAVVVIVAFTYGNPRFRAAAEPAVVVLAALGALDVVGAVVGRSAGVA
                     GAGAAEVDGIEGAEGGLVPLVEEDG"
     gene            complement(2426..4330)
                     /locus_tag="VNQ33_04465"
     CDS             complement(2426..4330)
                     /locus_tag="VNQ33_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97389.1"
                     /translation="MPADDPADARRGDRRWLLIAIVAAVPILVLIAALAQRTWYPTGD
                     QAQAELRMLSLPDHPPLVGAAGRIADEQFRQGNHPGPLMFWVTWPAYKLLGGSSWAFE
                     AATALVNAAWLGLSVWLVKRRAGTGACAWYGAVALVLIGGFGLDALSQPWNPWVALLP
                     FAVLLLTTWGAIEGDRWSPVIATVAGSYALQGHVGYLPLVLPLVGVALVAPLVRWWRA
                     PMVPAPDAGADPDRASDPASASRPRAAVWLLPLGAAVAVALFAWSGPLYDLLANDPSN
                     VAKIFANFSTPSDPPIGLQAGIEAVFQSTGPVGSWVWGGAAVEGPAWPGVLLVLAWLV
                     VAVLVAVRRTRPALTRFNVLLAGTLVIGMFAVSRIFGSLYLYTFRWIVVIVAFQVFTL
                     GWGIAVLLPRPSPDAQRRLAGLGVVGLVLLSAATAVRISRQEIPYDQSWRSEQVLAPA
                     VAAKLDPDRRYIVRWDDPAYLGGLGFGLILDLERRGFTVGGQPQFDAAIEPHRVMCSG
                     DFDAVVTVVTGEHRIAAWQARDGITELASTDPREDPAAWNRTADELVDVFNAQRPAGQ
                     DAMTRDSLENSLNLLLLAPGQSKEVVALASDLVLGGVPSAVFLDERDQATLGPLDAVD
                     LSSTRTSHPC"
BASE COUNT          640 a         1642 c         1636 g          596 t
ORIGIN      
        1 gtccccggcg agatcgacct gctgtacatc gacggtgcgc accgcttcaa gccggcgctc
       61 gacgacatca agcgctggag cgccaaggtc cgccccggtg gggacctgtt gatccacgac
      121 tcgttctcct ccgtgggcgt caccggtgcc atcggcgtct cgctgctcgc caacccgggc
      181 tggcgctacc tgggccgctc ggagtcgatg acccactacc ggcgcgagcc cctcacgagc
      241 aacgagcggg tccgcaacat cggacgccag ctcgcctcgc tgccgtactt cgccaagaac
      301 ctcgtcatca aggcgctgat cctcgccaag ctcgggttcc tcacccagcc gctcttccag
      361 cacgaccccg ccacgtggcc gcactgatga aggccgcgcc gatgacctcg ctcaccatcg
      421 tcatgcccgt gtacaacgag cgcgagacgc tccgcacggc gctcgaccgg ctgctggccg
      481 tcacgatgcc cgtcgagacc gaggtggtgg tcgtcgacga cggctccacc gacgggtgca
      541 ccgagaccat caccgacctg gtggagtccg gggcggtcac gctctacgtg cagaacccca
      601 accagggcaa gggcgcggcg ctgcgccggg gcatcgccga agccgagggc gacctgctca
      661 ccatcctcga cgccgacctc gagtacgacc cgtccgactt ccccgcgctg ctccagccga
      721 tcctcgacgg cgacgcccgg gtggtctacg gcgcccggtc ctacggcggc cacgccgcct
      781 actcgttctg gttcgtgctg gggaacaaga tgctcgccct ctgggcgtcg ttcctgttcg
      841 acgcctggct caccgacatc gagacctgcc tgaaggtggc ccccacgggc cactggcgca
      901 aggtcgatct ccggtcgaac ggcttcggca tcgaagccga gctcaccggc aagctcctga
      961 cgatgaacga gcgcatcttc gaggtgccga tctcgtatcg cgcacgcggg cgcgaggagg
     1021 gcaagaagat ccagtggacc gacggcgtcg ccgccctctg gatcctcctg cgcatccgcc
     1081 tccgggggac gtgaccgccg aggtccagcc ggcgccgcgc cggatcatcc cgcccgcccg
     1141 caccgccgcc ctgctgggcc tgctggtgct ggtgggcttc gtcatccgca tcgacgccct
     1201 gcgcaacagc gcgccggacg tccccgtgct gggcgacgcc cgggcgtacc acctgctggc
     1261 cgagaacctc gccgacggcc gcgggtacat caggccgtac gagttcgccg ccgacgggac
     1321 ggagatcaag acggcggagt acccgccggc gctgcccgtg gtgctcgccg ccgccacgaa
     1381 ggccggtgcc tcgtcggaga cctcgcagcg gctcctcctg tgcggtgtgg gcgcgctcac
     1441 ggtcgggctc gtcgggttga tcggccgccg cctcggcggc gacagcgcgg gctacctcgc
     1501 cgcgctcctc gccgccatcc acccggggct ctggaacagc gacgtctccc tgatggccga
     1561 gccgctggcc tcgttcacgg gggcggccct cgtcgtggcc gcgctctggg tcgtgaaccg
     1621 ccctgatcgg tggcgctggg ccgcgttcgg cgcggtggcc ggcctcggct gcttcgtgcg
     1681 gtccgagttc ctcctgatgg gcccggcgct catggccgtc gtggcgtgga agacggccga
     1741 ggcgtggcgg ccccgcctcg gccggctcgc cctgggcgtc ggggtgctcg tggcggtgct
     1801 cgtgccgtgg accgtccgga acctggtggc gttcgacggt cagctggtgc cgctgtcgaa
     1861 caactccggc tcggttgccc agggggccaa ctgcggtgcc gcctactccg gcccgttcaa
     1921 gggcctctgg gtcaccaacg tctccctcga cggcaacgac gccgaccaga gccgggccgg
     1981 ctgcttcgcc gggttcgacc tcaccgacga cgacgaggcc caggccgccg cccggctccg
     2041 ggccgacggc atcggctacc tgaccgacca caagggccag gtccccggcg tcgtcgccgc
     2101 ccggctcggc cggaccttcg ggctgtacca gttcgagcag cagcggaact tcgccggcgc
     2161 cgagggccgc aacgtcttgt gggacggccg ggggacccgc agcttccagc tgctcgccct
     2221 cgtcggcatc gccgggctcg tcgtctcgtc ggtccgccgc acgcgcacgt gggagcggtg
     2281 gctgctggtg gtgcccccgg ccgccgtggt cgtcatcgtc gccttcacct acggcaaccc
     2341 gcgctttcgc gccgccgcgg agccagccgt ggtggtcctc gccgcgctcg gcgcgctcga
     2401 cgtcgtcggc gccgtcgtgg gccgctcagc aggggtggct ggtgcgggtg ctgctgaggt
     2461 cgacggcatc gagggggccg agggtggcct ggtcccgctc gtcgaggaag acggctgagg
     2521 gcacgccgcc cagcacgagg tccgacgcca gggcgacgac ctccttgctc tgaccgggag
     2581 cgagcagcag gaggttgagg ctgttctcga ggctgtcgcg ggtcatggcg tcctgcccag
     2641 ccggccgctg ggcgttgaag acgtcgacca gctcgtcggc ggtgcggttc caggcggcgg
     2701 ggtcctcgcg ggggtcggtg gacgccagct cggtgatgcc gtcgcgggcc tgccaggcgg
     2761 cgatgcggtg ctcgccggtg acgacggtga cgacggcgtc gaagtcaccc gagcacatga
     2821 cccggtgggg ctcgatggcg gcgtcgaact ggggctggcc gccgacggtg aacccgcggc
     2881 gttcgaggtc gaggatgagg ccgaagccca agccgccgag gtacgccggg tcgtcccacc
     2941 ggacgatgta gcgccggtcc gggtcgagct tcgccgccac cgcgggcgcc aggacctgct
     3001 cggatcgcca cgactggtcg taggggatct cctggcgaga gatgcgcacg gcggtggcgg
     3061 cgctcagcag gacgaggccg acgacaccga gcccggcgag ccggcgctgc gcgtcgggcg
     3121 agggccgggg gaggaggacc gcgatccccc aaccgagcgt gaagacctgg aacgccacga
     3181 tcacgacgat ccagcggaac gtgtagaggt acagcgaccc gaagatccgc gacacggcga
     3241 acatcccgat caccaaggtg ccggcgagca agacgttgaa ccgggtgagc gccggtcggg
     3301 tgcgccggac cgccacgagc acggcgacca ccagccaggc caagacgagg agcacgcccg
     3361 gccacgccgg cccctccacg gcggcgccgc cccagaccca ggagcccacc ggccccgtgc
     3421 tctggaacac cgcctcgatg ccggcctgca acccgatggg cggatcagac ggcgtcgaga
     3481 agttggcgaa gatcttggcg acgttgctcg ggtcgttggc gagcaggtcg tagagcggcc
     3541 cggaccaggc gaagagggcc acggcgacgg ccgcgccgag cggcaggagc cagaccgcgg
     3601 cgcggggccg gctggcggag gccgggtcgc tcgcccggtc gggatccgca ccggcatcag
     3661 gtgccggcac catgggagcg cgccaccagc gcaccagggg tgcgaccagc gcgacgccca
     3721 ccagcgggag cacgaggggg aggtagccga cgtggccctg gagcgcgtag ctcccggcca
     3781 cggtggcgat cacgggcgac cagcgatcgc cctcgatcgc accccaggtg gtgaggagca
     3841 ggacggcgaa cgggaggagg gccacccacg ggttccaggg ctgggagagc gcatcgaggc
     3901 cgaacccgcc gatgagcacc agcgcgacgg cgccgtacca ggcgcatgcc ccggtgccgg
     3961 cccggcgctt caccagccac accgacaggc cgagccacgc cgcgttgacc agcgccgtcg
     4021 ccgcctcgaa cgcccacgac gaaccgccga gcagcttgta ggcgggccac gtcacccaga
     4081 acatgagcgg gcccgggtgg ttgccctggc ggaactgctc gtcggcgatg cgcccggcag
     4141 cccccacgag gggcggatgg tccggcagcg acagcatccg cagctcggcc tgggcttggt
     4201 caccggtcgg gtaccacgtg cgctgggcga gggcggcgat cagcaccagg atcggcaccg
     4261 cggccacgat cgcgatgagc agccaacggc ggtccccgcg gcgagcgtcg gcgggatcgt
     4321 cggcgggcac ggtgagccag ggtagccagg gggccgatct aggatgctcc gccatggctg
     4381 cgacgaccac cgccgcgtcg caagccgcgg tcgagccggg ttcgctgagc agccggaacc
     4441 cccagctcga gggtcttcgg gccgtcgccg ccctcgcggt gctggtcacg cacgtgtcgc
     4501 tcaacgccat gggc
//
LOCUS       DATMFS010000243         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_195336, whole genome shotgun sequence.
ACCESSION   DATMFS010000243 DATMFS010000000
VERSION     DATMFS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195336"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VNQ33_04470"
     CDS             <1..1977
                     /locus_tag="VNQ33_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ97390.1"
                     /translation="PDDLLAEPEDGVHIDRVLGTGPGRQRVLRVQTGDGSYKRLRMER
                     STSDELGDHLMLLTGREVQGERDAADLLSDQTVLLERIARGAPVGESLLAVADLAARR
                     LGDGEVLIGYFDGTSYLAESPSVDDELMAVLDRTGISRPPLQPPPPGADLVRTNEGWD
                     SVVKAASGGRCIRAFTVDLSGSEGLVGRLVFLRHHDEDLTPEERDLVALAVDLATIAV
                     DRHHLQARLAHGALHDELTGLPNRRFLLARLRDAFEAEGVRAGLLFVDLDRFKLINDS
                     LGHDAGDQLLQEVSYRFRRALRPADLVARVGGDEFVVLCPDLDSVDAVAALAARLTDA
                     LAAPIDLPGGRVVVSASIGVVHATGPADPAELLQDADLAMYDAKERGRNRTALFHVEL
                     RDRALARLEVENALRDALRADEMVLHFQPVVRLRDRTMVGVEALLRWERPGIGLVKPS
                     AFVPVATDTGLILPLGRWVIEQASRHAARWPSLEVAANLSARQLADADLVDFVADCLS
                     RHGVPPEQLCLEVTEADLVTDPDAIVEQLRRFKELGVRLAIDDFGTGFATLDYLRRFS
                     AADILKVDASFVAGLSDPSSHDLAIVSAALVLADNLGFDTVAEGVETAAQAEVLERLG
                     CELAQGHLFSPSITAEAIDDLLTRGAVFDGAGSR"
BASE COUNT          250 a          800 c          667 g          284 t
ORIGIN      
        1 cccgacgacc tgctggccga gcccgaggac ggcgtgcaca tcgaccgggt gctcggcacc
       61 ggacccggcc gccagcgcgt gctccgggtg cagacgggcg acggctcgta caagcggctc
      121 cgcatggagc gctcgaccag cgacgagctg ggcgaccacc tcatgctgct caccgggcga
      181 gaggtccagg gcgagcgcga cgccgccgac ctgctcagcg accagaccgt gctcctcgag
      241 cgcatcgccc ggggtgcgcc ggtcggggag tccctcctcg ccgtggccga cctcgccgcc
      301 cgccggctcg gcgacggtga ggtgctcatc ggctacttcg acggcaccag ctacttggcc
      361 gagtcgccct cggtcgacga cgagctgatg gcggtgctcg accgcaccgg catcagccgt
      421 ccgcccctcc agccaccgcc gcccggcgcc gacctcgtcc gcaccaacga aggctgggac
      481 tccgtcgtga aggcggcgtc cggcggccgc tgcatccgcg ccttcaccgt cgacctctcg
      541 gggtccgaag ggctggtcgg ccgactcgtg ttcctccgcc accacgacga ggacctcacc
      601 ccggaggagc gcgacctggt ggcgctggcg gtcgacctcg ccaccatcgc cgtcgatcgc
      661 caccacctgc aggctcggct cgcccacggc gcgctgcacg acgagctcac cggactgccg
      721 aaccgccgct tcctgctcgc ccggctgcgt gacgcgttcg aggccgaggg cgtccgcgcc
      781 ggtctgctct tcgtcgacct cgaccgcttc aagctcatca acgacagcct cggccacgat
      841 gccggcgacc agctgctcca ggaggtgagc taccgcttcc gccgcgccct gcgccccgcc
      901 gacctggtgg cgcgcgtcgg cggcgacgag ttcgtcgtgc tgtgccccga tctcgacagc
      961 gtcgacgccg tggccgcgct ggctgcccgc ctcaccgatg cgctcgcggc gccgatcgac
     1021 cttcccggcg gccgcgtggt ggtgtcggcc agcatcggcg tcgtccacgc caccggtccg
     1081 gccgaccccg ccgagctgct ccaagacgcc gacctcgcca tgtacgacgc caaggagcgg
     1141 ggccggaacc gcacggcgct gttccacgtc gagctccgcg accgcgccct ggcgcggctc
     1201 gaggtcgaga acgccctgcg cgacgcgctg cgcgccgacg agatggtcct gcacttccag
     1261 ccggtggtgc gccttcgaga ccggacgatg gtgggggtcg aggcgctgct ccggtgggag
     1321 cgtcccggca tcggtctcgt gaagcccagc gcgttcgtgc ccgtcgccac cgacaccggt
     1381 ctgatcctcc cgctcggccg ctgggtcatc gagcaggcct cccgccacgc ggcccgctgg
     1441 ccctcgctcg aggtggcggc caacctctcg gcccggcagc tggccgacgc ggacctggtc
     1501 gacttcgtgg ccgactgcct ctcccgccac ggcgtcccgc ccgagcagct gtgcctggag
     1561 gtcaccgagg ccgacctcgt caccgatccc gatgccatcg tcgagcagct ccgccggttc
     1621 aaggagctgg gggtccgcct cgccatcgac gacttcggca ccggcttcgc cacgctcgac
     1681 tacctgcgcc ggttctcggc cgccgacatc ttgaaggtcg acgcctcgtt cgtggccggt
     1741 ctcagcgatc cgtcgtcgca cgacctcgcc atcgtgtcgg ccgccctcgt cctcgccgac
     1801 aacctcggct tcgacacggt ggccgagggc gtcgagaccg cggcccaagc cgaggtgctc
     1861 gagcgcctcg gctgcgagct ggcccagggc cacctcttca gcccgtcgat caccgccgag
     1921 gcgatcgacg acctcctcac ccggggcgcc gtgttcgacg gcgccgggag ccgttgacga
     1981 gccgaccgct cgcgcccagg c
//
LOCUS       DATMFS010000244         5348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_311434, whole genome shotgun sequence.
ACCESSION   DATMFS010000244 DATMFS010000000
VERSION     DATMFS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311434"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VNQ33_04475"
     CDS             complement(<1..608)
                     /locus_tag="VNQ33_04475"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWJ97391.1"
                     /translation="MLSKVLGAVGRTLITAGTLILLFVAYQLWGTGLQEAKSQKVLSK
                     DFEEQLASIDQTATTTTAPPTTTTSTPEGTTPTIAVTKVEPVTQNLPLPAEGDPIAKI
                     EIPSIGVNRTVVSGVALSQLKRGPGHYAETPLPGQKGNVAIAGHRTTYGQPFHNIDKV
                     KKGDQIIFKTMQGTFVYEATDTLIVKPSQVEILEDKGDNRVT"
     gene            748..1422
                     /gene="ccmA"
                     /locus_tag="VNQ33_04480"
     CDS             748..1422
                     /gene="ccmA"
                     /locus_tag="VNQ33_04480"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWJ97392.1"
                     /translation="MDAVVRFRAAVCLLGRFPALAGVDLDVAAGEIVLLQGPNGAGKT
                     TLLRACAGLVPVTEGQAGVLGVDLVADRRPVRPLVGLLGHSTGLYDDLTVVENVRFWS
                     RAGRSQRADADAALARLGLDGRLASVPVARLSAGQRRRTALAVLVARRPRLWLLDEPH
                     AALDQDGRDLVDGLMVDAVAAGATVLFASHELDRAEAVAHRSVTVAGGTVNGLLAGAG
                     QEVPVA"
     gene            1415..2122
                     /locus_tag="VNQ33_04485"
     CDS             1415..2122
                     /locus_tag="VNQ33_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWJ97393.1"
                     /translation="MPDLAADSMLRGSMLRDAAMVVGKDLRLEWRSRVTLSQILPFAL
                     LVLILFAFALDPDRRILEEATPGLFWLAVLFSMLLAIQRAYAVETADGVIDALRLSDL
                     DPAGIFLGKAAGIGLHLLVLEVVLFVGVTVLYGATPHGGLLVVATMLLATTGLAAAGS
                     LYGVLAAGLRVRDTLLPILMLPVVAPVLIAGTEAFSAAYANASGDGWKWCGLLAAFSV
                     LYLAIGIFAFEPLLEES"
     gene            2119..2934
                     /gene="ccsA"
                     /locus_tag="VNQ33_04490"
     CDS             2119..2934
                     /gene="ccsA"
                     /locus_tag="VNQ33_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWJ97394.1"
                     /translation="MTSPSAGTGAGIMRLHPDGTASRGSRILGIVVLVSVVVFLYVAL
                     VASAPDKSLDAQGQLVRIMYLHVPSAIACYTAFFVTAVASGMYLWKKTVGWDALASSS
                     AEIGVLFVALTLLTGSIWGHTTWGTWWEWDPRLTSTALMFVLYLGYLALRRAIIDPVS
                     RAKQSAILGIIAFVNVPIVHYSVDWWRSLHQTATVASLDPKIDGLRLFALMFGMVVAL
                     LLYVWLMMHRFRLAFAEAALEAKGLDLALAERRAEARADGGSVPPSVTTGGAS"
     gene            2934..3092
                     /locus_tag="VNQ33_04495"
     CDS             2934..3092
                     /locus_tag="VNQ33_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97395.1"
                     /translation="MGVVLAEASAWTYVTATYAVTIGLLGGYTAWLIQKGRKVGRQLP
                     PEDRKWSS"
     gene            3080..3607
                     /locus_tag="VNQ33_04500"
     CDS             3080..3607
                     /locus_tag="VNQ33_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWJ97396.1"
                     /translation="MVVVTVDLPDDRDLDEGGLDLSPRDDAPVRAARTTRGRWAPIVV
                     LVLLGLVVGAIAMQARGASLYFKNADEAVAEKASLGTKRFRLQGTVVGKPVEKDVATE
                     FTLQYNGVSVEVVHTGSEPAMFKAGIPVVAEGHWNEAGTAFDSDRLLVKHDADYKDYD
                     EKHPERVDSDQKTDT"
     gene            3604..>5348
                     /locus_tag="VNQ33_04505"
     CDS             3604..>5348
                     /locus_tag="VNQ33_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWJ97397.1"
                     /translation="MNAALGFGGVVLGVVGCLGAIVTLAIGLKNHRPDLLRMSRTYAL
                     MILGAAVLSFFAMERALITRDFSLEFVATNGSTKTPALYNVATLWSALEGSILLWTLI
                     LAGYCVAVVLKFRKRLTDPLVGWAMLTMFVVALFFFGLMLGPANPFTTLAKVPLDGPG
                     PNPLLQNHPLMAFHPPMLYLGYVGFTVPFAFAVAALATGRLGEGWLVETRRWTLFAWS
                     FLTIGIILGAWWSHEVLGWGGYWAWDPVENASFLPWLTGTAFIHSVMVQERRGMLRVW
                     NLSLLCATFSLTILGTFLTRSGVLDSVHAFSDSTIGPLLLGFFALIVGVTVGLIAWRG
                     DRLRSPARIDSPASREGAFLLNNIVFAAFAFVVLLGTVFPLIAESLNGDRVTVGVPYF
                     ERMTMPIGIVLLFLMAIAPVLPWRKASVDTLRTRLLWPGWIGTGALVVALLLGARGFA
                     PLLAFFLAGFAAGSALRQLALATRRQGFRGLLGRTNGGMIVHLGVIMLAVAFTASNAY
                     QSEREARMCVTAKEGCPSTITVAGHDITYLGSADLSTAARTQISARLLVDGKVYRPGI
                     QQFPNGNQQIGKPTV"
BASE COUNT          647 a         1974 c         1795 g          932 t
ORIGIN      
        1 aaggtgacgc ggttgtcgcc cttgtcctcc aggatctcga cctggctggg cttcacgatg
       61 agcgtgtcgg tcgcctcgta gacgaaggtg ccctgcatcg tcttgaagat gatctggtcg
      121 cccttcttca ccttgtcgat gttgtggaag ggctggccgt aggtggtgcg gtggccggcg
      181 atggcgacgt tgcccttctg gcccggcagc ggcgtctccg cgtagtggcc gggcccccgc
      241 ttgagctggc tcagggccac ccccgacacg acggtgcggt tgacgccgat ggacgggatc
      301 tcgatcttcg cgatgggatc gccctcggcc ggcagcggca ggttctgggt gaccggctcg
      361 accttggtca ccgcgatggt cggcgtggtg ccctcgggcg tgctcgtggt ggtggtcggc
      421 ggggccgtgg tggtcgtggc ggtctggtcg atcgacgcca gctgctcctc gaagtccttc
      481 gagagcacct tctgcgactt ggcctcctgc aggcccgtgc cccacagctg gtatgccacg
      541 aacagcagga tcagggtccc ggccgtgatc agcgttcttc cgactgcacc gaggaccttg
      601 gagagcacgg ggggtgagac tacgtgaggg accggaaagt tcccgagcgc cggacccgtg
      661 aatcatgcca cgtgccgggg ccgtcatgga ccgggtcgat ccgggttcgt caccggctcc
      721 gggccgccgg taccgtttgt ggtgcccatg gacgccgtcg tcagattccg cgccgcggtc
      781 tgtctgctcg gtcgctttcc cgcactggcc ggcgtcgacc tcgacgtggc cgccggcgag
      841 atcgtgctgc tgcaaggacc caacggtgcc ggcaagacca cgttgctgcg cgcctgcgcg
      901 ggcctggtcc cggtcaccga ggggcaggcc ggcgtcctcg gcgtcgacct ggtggccgac
      961 cggcgcccgg tccgcccgct ggtcggcctc ctcggccaca gcaccgggct ctacgacgat
     1021 ctgaccgtgg tcgagaacgt ccgcttctgg tcgagggccg gccggtcgca gcgcgccgac
     1081 gccgatgccg ccctcgcccg cctcgggctc gacggccgcc tcgccagcgt cccggtcgcc
     1141 cgcctgtccg ccgggcagcg gcgccggacc gcgctggccg tcctcgtcgc ccgccgcccc
     1201 cgcctctggc tgctcgacga gccccatgcc gcgctcgacc aggacggccg cgacctcgtc
     1261 gacggcctga tggtcgatgc cgtcgccgcc ggtgccaccg tgctgttcgc ctcccacgag
     1321 ctcgatcgcg ccgaggccgt ggcccaccgg tcggtcaccg tggccggcgg caccgtcaac
     1381 ggcctcctcg ccggcgccgg acaggaggtg cccgttgcct gacctcgccg ccgactcgat
     1441 gctgcgcggt tcgatgctgc gcgatgccgc catggtggtc ggcaaggacc tccgactgga
     1501 gtggcgcagc cgcgtcacgc tcagccagat cctcccgttc gcgctgctgg tgctgatcct
     1561 cttcgccttc gccctcgacc ccgatcgccg gatcctcgag gaggcgacgc cgggcctgtt
     1621 ctggctggcc gtgctgttct cgatgctcct cgccatccag cgggcctacg ccgtggagac
     1681 cgcggacggc gtcatcgacg ccctccgcct ctcggatctc gatccggccg ggatcttcct
     1741 cggcaaggcg gccggcatcg gcctgcacct gctcgtgctc gaggtcgtgc tgttcgtcgg
     1801 cgtgaccgtg ctctacggcg ccacgcccca cggcggcctg ctcgtcgtgg ccaccatgct
     1861 cctcgccacc accggactgg ccgccgccgg cagcctctac ggcgtgctcg ccgccggcct
     1921 gcgggtgcgg gacacgctcc tgcccatctt gatgctcccc gtcgtggccc cggtgctgat
     1981 cgccgggacc gaggccttct ccgccgccta cgccaacgcg tcgggcgacg gatggaagtg
     2041 gtgcggcctg ctggctgctt tctccgtgct ttacctcgcg atcgggatct ttgccttcga
     2101 acccctcctg gaggaatcgt gacgtccccc tcagccggca ccggtgccgg catcatgcgc
     2161 ctgcacccgg acggcaccgc gtcgcgcggc agccggatcc tcggcatcgt cgtgctggtc
     2221 tccgtggtgg tgttcctcta cgtggccctg gtcgcctcgg cgccggacaa gagcctcgac
     2281 gcccagggcc agctcgtgcg gatcatgtac ctgcacgtgc cctcggccat cgcctgctac
     2341 acggcgttct tcgtgacggc ggtcgccagc gggatgtacc tctggaagaa gaccgtgggc
     2401 tgggacgccc tggcgtcgtc gtcggcggag atcggcgtgc tgttcgtggc gctgaccctg
     2461 ctgaccggtt cgatctgggg ccacaccacc tggggcacct ggtgggagtg ggacccgcgc
     2521 ctcaccagca cggcgctgat gttcgtgctc tacctcggct acctggcgct gcgccgggcg
     2581 atcatcgacc cggtctcgcg ggcgaagcag tccgccatcc tcggcatcat cgccttcgtc
     2641 aacgtgccga tcgtccacta ctcggtcgac tggtggcgca gcctccacca gacggccacc
     2701 gtggccagcc tcgatccgaa gatcgacggg ctgcgcctct tcgccctgat gttcggcatg
     2761 gtcgtggccc tgctgctgta cgtctggctg atgatgcacc gcttccgcct ggccttcgcc
     2821 gaggcggcgc tggaggcgaa gggcctggac ctggcgctcg ccgagcgccg ggccgaggcc
     2881 cgggccgacg gtgggtcggt cccgccgtcc gtgaccaccg ggggagcgtc gtgatgggcg
     2941 tcgtgctcgc cgaagccagc gcatggacct acgtgaccgc cacctacgcg gtcaccatcg
     3001 ggctgctggg cggctacacc gcctggctca tccagaaggg ccgcaaggtc ggcaggcagc
     3061 tgccgcccga ggatcggaag tggtcgtcgt gaccgtcgac ctccccgacg accgggacct
     3121 cgacgagggc gggctcgacc tcagcccccg cgacgacgct ccggtccgcg ccgcccgcac
     3181 cacccgcggc cgctgggcgc ccatcgtcgt gctcgtcctg ctcgggctgg tcgtgggcgc
     3241 catcgccatg caggcccggg gcgcgtcgct gtacttcaag aacgccgatg aggccgtggc
     3301 cgagaaggcg agcctcggca ccaagcggtt ccggctccag ggcaccgtcg tcggcaagcc
     3361 cgtcgagaag gacgtcgcca ccgagttcac cctccagtac aacggcgtga gcgtcgaggt
     3421 cgtccacacg ggcagcgagc cggccatgtt caaagccggc atccccgtcg tggccgaggg
     3481 ccactggaac gaggccggga ccgcgttcga cagcgaccgc ctgctggtca agcacgacgc
     3541 ggactacaag gactacgacg agaagcaccc cgaacgcgtc gacagcgacc agaagaccga
     3601 cacgtgaacg ccgccctcgg gttcggcggc gtcgtcctcg gcgtcgtcgg gtgcctcggc
     3661 gccatcgtca ccctcgccat cggcctgaag aaccaccgcc ccgacctgct gcgcatgtcg
     3721 cgcacctatg cgctcatgat cctgggcgcg gcggtgctgt cgttcttcgc catggagcgg
     3781 gcgctcatca cccgggactt ctcgctcgag ttcgtggcca ccaacgggtc gaccaagacg
     3841 ccggcgctct acaacgtcgc cacgctctgg tcggcgctcg aagggtcgat cctgctgtgg
     3901 acgctgatcc tcgccggcta ctgcgtcgcc gtcgtcttga agttccgcaa gcggctcacc
     3961 gacccgctcg tcggctgggc gatgctcacc atgttcgtgg tggcgctgtt cttcttcggg
     4021 ctgatgctcg gcccggcgaa cccgttcacg acgctggcca aggtgccgct cgacggtccc
     4081 ggcccgaacc cgctgctgca gaaccacccg ctgatggcgt tccacccgcc gatgctctac
     4141 ctcgggtacg tcggcttcac ggtgccgttc gcgttcgccg tcgccgcgct cgccaccggc
     4201 cggctggggg aggggtggct ggtcgagacg cggcgctgga cgctgttcgc ctggtcgttc
     4261 ctcaccatcg gcatcatcct cggtgcctgg tggtcccacg aggtcctcgg ctggggcggg
     4321 tactgggcgt gggacccggt ggagaacgcc tcgttcctcc catggctcac cggcaccgcc
     4381 ttcatccact cggtgatggt gcaggagcgc cgcggcatgc tgcgcgtctg gaacctgtcg
     4441 ctgctgtgcg ccacgttctc gctcacgatc ctcggcacgt tcctcacccg gtccggggtg
     4501 ctcgattcgg tccacgcctt cagcgattcg accatcggcc cgctgctcct cgggttcttc
     4561 gccctcatcg tcggcgtcac ggtcgggctc atcgcctggc ggggcgaccg cctccgctcg
     4621 ccggcccgca tcgactcgcc ggcgtctcgc gagggcgcct tcctgctcaa caacatcgtg
     4681 ttcgcggcgt tcgcgttcgt cgtgctgctc ggcaccgtgt tcccgctgat cgccgagtcg
     4741 ctcaacggcg accgcgtcac cgtcggcgtg ccctacttcg agcggatgac gatgcccatc
     4801 gggatcgtcc tgctgttcct catggccatc gccccggtcc tgccgtggcg caaggcgtcg
     4861 gtcgacacgc tgcgcacccg gctgctctgg cccggctgga tcggcaccgg cgcactcgtc
     4921 gtggccctgc tgctcggcgc ccgcgggttc gccccgctgc tcgccttctt cctggccggc
     4981 ttcgcagcgg gatcggcgct gcgccagctg gcgctggcca cccgccgcca ggggttccgc
     5041 ggcctgctcg ggcgcaccaa cggcgggatg atcgtgcacc tcggcgtgat catgctggcc
     5101 gtggccttca ccgcgtcgaa cgcgtaccag tccgagcgcg aggcccggat gtgcgtcacc
     5161 gccaaggagg ggtgcccgtc caccatcacg gtggccggcc acgacatcac ctacttggga
     5221 tcggccgacc tctcgaccgc ggcccgcacc cagatcagcg cccgcctcct cgtcgacggc
     5281 aaggtctacc ggcccgggat ccagcagttc ccgaacggaa accagcagat cggcaagccg
     5341 actgtgaa
//
LOCUS       DATMFS010000245         2978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_78935, whole genome shotgun sequence.
ACCESSION   DATMFS010000245 DATMFS010000000
VERSION     DATMFS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78935"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VNQ33_04510"
     CDS             complement(<1..241)
                     /locus_tag="VNQ33_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97398.1"
                     /translation="MSGFCAVLVSVRADRLVAGPAHPEAKSALRKLVRTLPEPRRERA
                     EAASDAVVFDPTTWDRRDPALPEPAHLGGVQRAVID"
     gene            complement(313..765)
                     /locus_tag="VNQ33_04515"
     CDS             complement(313..765)
                     /locus_tag="VNQ33_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97399.1"
                     /translation="MTVRFRPATPADLPAVVALLHDDALGARREHSELAPYEAAFAEI
                     EADSNHLVVVGTVDDEVVACLQATALPCLTHGGRRRAQLEGIRVASGHRGGGIGAALV
                     RWAIDWAREQRCAIVQLTTDKERPDALRFYESLGFAPTHQGCKLVLEP"
     gene            complement(1163..2041)
                     /locus_tag="VNQ33_04520"
     CDS             complement(1163..2041)
                     /locus_tag="VNQ33_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97400.1"
                     /translation="MTTGLLANPAHAEDATDPAAVEAAATANIAAGQSGISQEVTAEG
                     TSADLGNGAVEVSTDPSDGLTVTAPTGTDISLGVPGETDDGTVVGSNVVYPDVATDAA
                     VVARATPEGAQALIVIDGNSAPERYTFPIEVNGQDTVLRESDDGGIEVISPDSTDPVA
                     TVAPPWATDAHGAPVSTHYEIEGSSIIQVVDHHGAAYPVTADPKYSWGYVTGTAYYNR
                     KETRSLKTRSYAYVVAAGLCAAFGGATAGAACAVGAAVAAQWNYVASNAYGDGKCVKI
                     KMPIMWAYAYSGGNCK"
     gene            complement(2231..>2978)
                     /locus_tag="VNQ33_04525"
     CDS             complement(2231..>2978)
                     /locus_tag="VNQ33_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ97401.1"
                     /translation="ALDPAPFGPVFRSRPAARLTRNGIQRIIDTNLDRAGLTDPTRLD
                     EGARRQISTAFPGGRSEDIRDRALIANLYWGCFRASELTQRDWRHLKIVTEGIEWTIP
                     NDKTNQEGRQRRVTGVPRHNDPFLCPVLALEEWRTHLETLIDRPVKASDPVFPTLDRR
                     TRLTAHMSVQAANHAVQQAAHRAGLDGDYGSHSLRSGFTTDALDGDVTRDQIRHHGGW
                     RNSQSLDPYIRRSATWARTNPAIQMLNRES"
BASE COUNT          416 a          867 c         1173 g          522 t
ORIGIN      
        1 ggtcgatgac ggcccgctgg acaccaccga ggtgggctgg ctccgggagg gctgggtccc
       61 gtcggtccca ggtggtgggg tcgaacacga ccgcatcgga ggcggcctcg gctcgctcgc
      121 gccgcggttc agggagggta cggaccagct tgcgcagggc gctcttggcc tcggggtgag
      181 ccgggccggc gacgaggcga tcggcgcgca cggagacgag gaccgcacag aaaccggaca
      241 ccagatggcc gcttcaccgc gcggaggcgg cggcatgatc aacgacgccc tgctccacga
      301 gcaggcggac cgctacggtt cgaggacgag cttgcagccc tggtgggtgg gggcgaagcc
      361 gagcgactcg tagaaccgga gggcgtcggg ccgctccttg tcggtggtga gctgcacgat
      421 ggcgcaccgc tgctcccggg cccagtcgat ggcccagcgc acgagcgccg cgccgatccc
      481 gccgccgcgg tgaccgctgg cgacccggat gccttcgagc tgggcccggc ggcgaccacc
      541 gtgggtcaag caggggagcg cggtggcctg caggcaggcg accacctcgt cgtcgacggt
      601 gcccacgacc accaggtggt tcgagtcggc ctcgatctcg gcgaaggcgg cttcgtacgg
      661 agcgagctcg gagtgctcgc gtctcgcgcc gagggcgtcg tcgtggagca gcgctacgac
      721 ggccgggagg tcggccgggg tggccgggcg gaaccggacg gtcaccgcaa cccccggatc
      781 aggaaccggt ggtgctccat tctgcggagc ttgggttcac caggttccga gaacgtgggc
      841 ttggcttgca catcttgtgg gtggcttcga atccgtgacg acggtctctg aggagggacg
      901 gcgtcgaggg gacgctgcga gtgctgcgtc ccctcgacgg ttgttttcag gcgttgccgc
      961 ccctgggcgg ggccgagtga atcggagcgt cgctatcccc acatccgaag tgacacgagg
     1021 accacgatta cagcgattcc tccaactcgg atcgctggac tcgccttggc gaaccgctcg
     1081 cctccagcga gagctctcac gccgaacaca agcacgatcg caaggagcgt cagcacgacg
     1141 aacgggagga gggtccccca actcacttgc agttgcctcc ggagtacgcg tatgcccaca
     1201 tgatgggcat cttgatcttg acgcacttgc cgtcgccgta ggcgttgctg gcgacgtagt
     1261 tccactgcgc ggcgactgcg gccccgacag cacatgcggc gccggcggtg gcgccgccaa
     1321 acgctgcgca caagccagcc gccacgacgt atgcgtagga gcgggtcttg agcgacctgg
     1381 tctccttccg gttgtagtac gcggtgccgg tgacatagcc ccacgagtac ttcgggtcag
     1441 cggtgacggg gtaggcggcg ccgtggtggt caacgacctg gatgatcgag gaaccctcga
     1501 tctcatagtg ggtcgagacc ggggcgccgt gagcgtcggt ggcccaaggc ggggcgacag
     1561 tcgcaacagg atcggtgctg tcgggactga tgacctcgat gcccccgtcg tcgctctcgc
     1621 ggagaacggt gtcctggccg ttgacttcga tggggaacgt gtagcgctcg ggcgcgctgt
     1681 tgccgtcgat gacgatgagc gcctgggctc cttcgggggt agcccgggcg acgacggctg
     1741 cgtcggttgc gacgtcgggg tagacgacgt tgctgccgac gacggtgccg tcgtcagttt
     1801 cgccagggac gccaagcgag atatcggtgc cggtcggagc ggtgaccgtc agaccgtcgc
     1861 tcggatcggt ggagacctcg acggctccat tgccaaggtc ggcggaggtt ccctccgcgg
     1921 tgacttcctg ggagatcccg gattgtcctg ctgcaatgtt cgctgtcgct gctgcttcga
     1981 cggctgcggg gtcggtggcg tcctcggcgt gtgctgggtt ggccagcagg ccggtggtca
     2041 tgctgagcgc tgcggcgagg ccgagtgctc ggatcgaatg gtgctttccc acgtcatctc
     2101 cacctgttgt gaggccggag gtcggtctcg ggtcagttgg tgtcgacgtg gggtggaaac
     2161 catgtggtcc gccgacgggt gggagggtag ctgagggccg atgtcggtgc ttcgggatgg
     2221 cggcgcggtg tcacgactct cggttgagca tctggatggc ggggttggtg cgggcccacg
     2281 tggctgatct acggatgtag gggtcgaggc tctggctgtt gcgccagccg ccgtggtgcc
     2341 tgatctggtc gcgggtgacg tcgccgtcga gggcgtcggt ggtgaaccct gaccggaggg
     2401 agtggctgcc gtagtcaccg tcgagcccgg cgcggtgggc ggcttgttgg acggcgtggt
     2461 tggcggcctg gacgctcata tgggcggtga ggcgggtgcg ccggtcgagg gtggggaaca
     2521 ccgggtcgct ggctttgacg gggcggtcga tgagggtttc gaggtgggta cgccattcct
     2581 cgagagcgag gacggggcag aggaacgggt cgttgtggcg ggggacgccg gtgactcgcc
     2641 gctggcggcc ttcctggttg gtcttgtcgt tggggatggt ccactcgatg ccttcggtga
     2701 cgatcttgag gtggcgccag tcgcgttggg tgagctcgga tgctcggaag cagccccagt
     2761 agaggttggc gatgagcgcc cggtcacgga tgtcttcgga gcgtccgccg gggaaggcgg
     2821 tgctgatctg tcgacgggcc ccttcgtcca ggcgggtggg gtcggtgagg ccggcccggt
     2881 cgaggttggt gtcgataatc cgttggatgc cgttgcgggt caggcgggcg gcggggcggg
     2941 atcggaacac gggtccgaag ggggcggggt cgagggct
//
LOCUS       DATMFS010000246         3996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_54931, whole genome shotgun sequence.
ACCESSION   DATMFS010000246 DATMFS010000000
VERSION     DATMFS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54931"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..92
                     /locus_tag="VNQ33_04530"
     tRNA            18..92
                     /locus_tag="VNQ33_04530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Arg,seq:ccg)
     gene            complement(156..725)
                     /locus_tag="VNQ33_04535"
     CDS             complement(156..725)
                     /locus_tag="VNQ33_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWJ97402.1"
                     /translation="MNWFRCSSAAALLVGAAAVLVPVLRSSDHPTGHGAPGYRTSRSA
                     DDVTGADRSTTTARPAAHALDPGLVAAYERARVAAAGAGKDLVINSGYRTPAYQQQLL
                     DEEVAKRGSFAEANRWVFTPERSMHVQGLAIDVGDGPAADWLVEHGARFGLCHTLGWE
                     WWHFEWRQRWQDSGSCPPPADDPSEAPGV"
     gene            911..2194
                     /locus_tag="VNQ33_04540"
     CDS             911..2194
                     /locus_tag="VNQ33_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWJ97403.1"
                     /translation="MFSHVLGRADSPRTPVLSHRPARHPLATDAAIGVGVAAVWLVVF
                     EIGKRTGWRPVDPRAWVIAGACLGLAVAVRRVGGVGLFVLLALAYPLVYGRPLLSYFH
                     LLPLLVLGFAGAQMGGRRHRVVLVVGACAVSVAILLSPLMTPRLWSPDRPGFEPPGPR
                     PRINLSDLALAEFATVSVALLGAAIASQRAAARDLADRNEELERLRRSEADRAVAEER
                     LRISREVHDLVAHHLTAILMRAQAADRVRDSRPDEAQRAVAWIADESREALRSTRLTV
                     STLREAASAAPGDRDDGPLADDLAQMVERVRQVGLHARLEARWDAEPRLSPSAGVAVR
                     RTVQEALTNVLKHAHATDALVLLRPDGGGLSTWVLDNGRATAPPGPPVPGSGAGLTGM
                     SERALACGGRVDHGPAPDGGWQVHLWVPILIGGER"
     gene            2191..2859
                     /locus_tag="VNQ33_04545"
     CDS             2191..2859
                     /locus_tag="VNQ33_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ97404.1"
                     /translation="MRAIRVLVVDDQEMIRAGIRLMVDHEPDLEVVGEAGDGAQAQEA
                     VASLAPDVVVMDIRMPVVDGIEATRRIVASGSPSAVLVLTTFDDDEFVFGALRAGAAG
                     FLLKDSPPDDLLNAIRAVNEGGSLVDPGLTRRLIERWSALEDAAKPEPARVELAAITP
                     REREILVGLARGWSNRELADNFFLSEGTIKSHVSSLLSKLGLRSRVQAVIYAYEAGVV
                     RVGE"
     gene            complement(2999..>3996)
                     /locus_tag="VNQ33_04550"
     CDS             complement(2999..>3996)
                     /locus_tag="VNQ33_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97405.1"
                     /translation="RAKAPLRADFFGDTLVGPTILQHGTEEQKKHFLPKIMKGEMAWC
                     QGFSEPDSGSDLASLKTSAVLDGDEWVINGQKVWTTQAQFADYCFLLARTDPDAPKHK
                     GISYLLVPMKQDGIEVRGIVQPDGTAEFNEVFFTDARCPRDNVVGGLNDGWKVANSTL
                     SHERGMSATTGYRRFQQELDYMVEEAQRNGRIDDPIVRQGLARYYSKIQILRINGLRS
                     LTQTLNGTKDLGVISLGLTNKMFWSEMHRNAMELALDIFGAASMLADTGPESGGWPAV
                     MRAKGRDGYKPSPMMSSFFFSRSETIWGGTAEIQRNIVGERFLGLPREPKPQASS"
BASE COUNT          529 a         1441 c         1436 g          590 t
ORIGIN      
        1 ctagggtgac ggcccctgcc ctcgtagctc aggggataga gcatcgccct ccggaggcgt
       61 gtgcgcaggt tcgaatcctg ccgagggcac cacgtgaacg gcccggcctc agcgccgggc
      121 cgttccgcgt cgaggccacg cagcgggccc gcacctcaga cccccggcgc ctcggacggg
      181 tcgtcggcgg gcggtgggca cgagcccgag tcctgccagc gctggcgcca ctcgaagtgc
      241 caccactccc agcccagggt gtggcacagc ccgaatcggg ccccgtgctc cacgagccag
      301 tccgcggccg gcccgtcacc gacgtcgatg gccaggccct gcacgtgcat cgaccgctct
      361 ggggtgaaca cccagcggtt ggcctccgcg aacgagcccc gcttcgccac ctcctcgtcg
      421 agcagctgct gctggtacgc cggcgtccgg tacccggagt tgatcaccag gtccttgccg
      481 gcaccggccg ccgccactcg tgcccgctcg taggcggcga cgaggccggg gtccaaggcg
      541 tgcgccgccg gccgtgccgt ggtcgtcgat cggtccgccc cggtcacgtc gtcggccgat
      601 cgggacgtcc ggtagccggg agccccgtgc cccgtcgggt ggtcggagct ccgcaggacg
      661 ggcacgagga cggcggcggc accgacgagc agcgcagcgg cggacgaaca gcggaaccag
      721 ttcacggcga cgatcgtccg cgctcgccgt cgccagcaca tcggcccgga ggccgcttcc
      781 ccgccgccga aaggcaggac cgcagcttcg cgatgccagg accaggatcg gccgttcggc
      841 cgaggtccac ccaccggcat cggccgttcg gccgatgccg gtgggtggcg tcgtcggtag
      901 ggtccccgag gtgttctcgc acgtcctggg ccgcgcggac agcccccgca cgccggtcct
      961 gtcgcaccgc ccggcccggc accccctcgc caccgatgcc gccatcggcg tcggtgtggc
     1021 agcggtgtgg ctcgtcgtgt tcgagatcgg gaagcggacg ggctggcgac cggtcgaccc
     1081 gcgggcgtgg gtcatcgccg gtgcctgcct cgggttggcc gtcgccgttc ggcgcgtcgg
     1141 cggcgtcggc ctgttcgtcc tgctcgccct cgcctacccg ctggtgtacg gacggccgct
     1201 gctcagctac ttccacctcc tgccgctgct cgtcctcggg ttcgccggcg cgcagatggg
     1261 cggccggcgg catcgggtgg tgctggtcgt cggggcctgc gccgtatccg tcgccatcct
     1321 cctgtccccg ctgatgaccc ctcgcctctg gtcacccgac cgtccggggt tcgagccgcc
     1381 tggcccgcgg ccccgcatca acctgtccga tctggccctc gccgagttcg ccacggtcag
     1441 cgtcgccctg ctgggtgcgg ccatcgcctc gcagcgagca gcggctcgcg acctcgccga
     1501 tcgcaacgaa gagctcgaac gcctgcggcg gtccgaggcc gaccgcgccg tcgccgagga
     1561 acggctccgc atctcccgcg aggtccacga cctcgtggcc caccacctga cggcgatcct
     1621 gatgcgggcc caggcggccg atcgcgtgcg cgactcccgg cccgacgagg cccagcgggc
     1681 ggtcgcctgg atcgcggacg agagccgcga ggcgctgcgg tccacccgcc tgaccgtctc
     1741 gaccctccgc gaggccgcct cggcagcgcc cggcgaccga gacgacggac ctctcgccga
     1801 cgacctggcc cagatggtgg agcgggtgcg ccaggtcggt ctccacgccc ggctggaagc
     1861 ccggtgggat gccgagcctc ggctgtcgcc gtcggccggg gtagccgtcc gccggacggt
     1921 ccaagaggcg ctgaccaacg tgctcaagca cgcccacgcc accgacgccc tggtgctgct
     1981 ccggcccgac ggcggcggcc tgagcacctg ggtgctggac aacggccggg ccaccgctcc
     2041 acccggtcct ccggtgccgg gcagcggggc cggactgacc ggcatgagcg agcgggcgct
     2101 ggcctgcggg ggccgggtcg accacggccc cgcgccggac gggggctggc aggtgcacct
     2161 gtgggtgccg atcctgatcg gcggtgagcg gtgagggcga tccgggtcct cgtcgtcgac
     2221 gaccaggaga tgatccgcgc cgggatccgg ctgatggtcg accacgagcc ggacctggag
     2281 gtggtgggcg aggcgggcga cggcgcccag gcccaagagg ccgtcgcctc gctcgctccc
     2341 gacgtggtgg tgatggacat ccgcatgccc gtggtcgacg gcatcgaagc gacccgccgc
     2401 atcgtcgcct cgggatcgcc cagcgccgtc ttggtgctca ccacgttcga tgacgacgag
     2461 ttcgtgttcg gtgccctgcg ggccggtgcc gccggcttcc tcctcaaaga cagcccgccc
     2521 gacgatctgc tcaacgccat ccgggccgtg aacgaaggcg gctcgctcgt cgacccaggc
     2581 ctcacccgcc ggctgatcga gcgctggtcg gctctcgaag atgcggccaa gcccgagccg
     2641 gcccgcgtcg agctcgcggc gatcacccct cgcgagcgcg agatcctcgt cgggctggcg
     2701 agggggtgga gcaaccgcga gctggccgac aacttcttcc tcagcgaagg gacgatcaag
     2761 tcccacgtga gcagcctgct gtcgaagctc ggcctgcgca gccgggtcca ggccgtgatc
     2821 tacgcctacg aggccggcgt cgtccgcgtc ggcgagtgag gccggcgact ccttcggtgc
     2881 gggctcaggt cccgaggaag gcccaggaag cgctcacccg ccgccgttgc gccatacgtg
     2941 ttgcgctgga tctcccggcg aagccgccat cacacccgaa cccgccaaca agccgacctc
     3001 acgacgacgc ctgcggcttg ggctcgcgag ggagaccaag gaagcgctcg cccacgatgt
     3061 tgcgctggat ctcggcggtg ccgccccaga tggtctcgga ccggctgaag aagaacgacg
     3121 acatcatcgg gctgggcttg tagccgtcgc ggcccttggc ccgcatgacc gcgggccacc
     3181 cgccggactc cggaccggtg tcggcgagca tggaggcggc gccgaagatg tcgagcgcca
     3241 gctccatcgc attgcggtgc atctcggacc agaacatctt gttggtgagg ccgagcgaga
     3301 tgacgcccag gtccttcgtc ccgttgagcg tctgggtcag cgagcgcagg ccgttgatcc
     3361 gcaggatctg gatcttcgag tagtagcggg ccaggccctg gcggacgatc ggatcgtcga
     3421 tccggccgtt gcgctgggcc tcttcgacca tgtagtcgag ctcctgctgg aagcggcggt
     3481 agccggtggt ggccgacatg ccccgctcgt ggctgagggt ggagttggcg accttccagc
     3541 cgtcgttcag gccgccgacg acgttgtcgc gcggacagcg ggcgtcggtg aagaacacct
     3601 cgttgaactc ggcggtgccg tcgggctgga cgatgccgcg gacttcgatg ccgtcctgct
     3661 tcatcggcac cagcaggtac gagatgccct tgtgcttggg ggcatcgggg tcggtgcggg
     3721 cgaggaggaa gcagtagtcg gcgaactggg cctgggtggt ccagaccttc tggccgttga
     3781 tgacccactc gtcgccgtcg agcaccgcgc tcgtcttgag cgaggccagg tcggagccgg
     3841 agtcgggctc ggagaagccc tggcaccagg ccatctcgcc cttcatgatc ttggggagga
     3901 agtgcttctt ctgctcctcg gtgccgtgct ggaggatggt cgggcccaca agggtgtcgc
     3961 cgaagaagtc ggcgcgcagc ggggccttgg cccggg
//
LOCUS       DATMFS010000247         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_263747, whole genome shotgun sequence.
ACCESSION   DATMFS010000247 DATMFS010000000
VERSION     DATMFS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263747"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VNQ33_04555"
     CDS             complement(<1..903)
                     /locus_tag="VNQ33_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HWJ97406.1"
                     /translation="MTIDDQDLRALVPQVLSVLVGRGADFATAEDAVQEALIRALRSW
                     SDDPPRDPKAWLVTVSWRVFLDMARSSSARLEREMRVDAEPLPGAVPTVDDTLRLYFL
                     CAHPDLTPSSAVALTLRAVGGLTTRQIAEAYLVPEATMAQRISRAKRTVADAGVDQPG
                     DAATVMRVLYLVFNEGFSGDVDLAAEAIRLTRQLAAAMDDAEVRGLLALMLLHHARRA
                     SRTGPDGRLIPLADQDRSRWDTLLIAEGVAVLQAALARDHLGQYQAQAAIAALHADAP
                     RVDETDWVQIVEWYDELLRFADTPV"
     gene            complement(916..1326)
                     /locus_tag="VNQ33_04560"
     CDS             complement(916..1326)
                     /locus_tag="VNQ33_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWJ97407.1"
                     /translation="MAKYLMLKHYRTTTPTPNEFASMDQWTPDEVEAHFQFMRDYAAR
                     LEATGEMVGGEGLSPDGVWVQAGREGKPPVVDGPFAETKDLIAGWFIIDVDSYERAVE
                     LAGELSAAPGPGGEPIYEWLELRPIMSAPPTFDE"
     gene            complement(1600..2085)
                     /locus_tag="VNQ33_04565"
     CDS             complement(1600..2085)
                     /locus_tag="VNQ33_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ97408.1"
                     /translation="MTFTPHTLNPELDLELVREIDVAPELVWRAWTEPELIEQWFAPA
                     PWAIAEASVDLRPGGAMAMTMRSPEGEEFPSVGCYLEVVPNERLVWTESMDPGFRPKT
                     IDLPLTAILELERLPNGGTRYRAVALHQDPAGVKQHQEMGFDEGWNAALDQLIALMSD
                     Q"
     gene            complement(2082..2426)
                     /locus_tag="VNQ33_04570"
     CDS             complement(2082..2426)
                     /locus_tag="VNQ33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWJ97409.1"
                     /translation="MPKRSDGIDEVLHALADPTRRQVVERLVSGPASTSELAEPFAMA
                     LPSFTQHLGVLERAGLVTSTKEGRVRTYRIARTALELVDGWLAEQRRIWERKLDQLDG
                     LLLNLNLKEQPT"
     gene            2578..>2827
                     /locus_tag="VNQ33_04575"
     CDS             2578..>2827
                     /locus_tag="VNQ33_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97410.1"
                     /translation="MGRYFTNDEYEAQFGRTLYAAMVDSADLGEAAVTADRIVAGGED
                     DAAWYREWTATAEGVVADAERSRAAGNDVDARSALLRAA"
BASE COUNT          410 a          957 c         1068 g          392 t
ORIGIN      
        1 gacgggcgtg tcggcgaagc ggaggagctc gtcgtaccac tcgacgatct ggacccagtc
       61 ggtctcgtcg acccgcgggg cgtcggcgtg cagcgcggcg atggcagcct gggcctggta
      121 ctggccgagg tgatcccggg cgagcgcggc ctggaggacc gcgacgcctt cggcgatgag
      181 cagggtgtcc cagcggctgc ggtcctggtc ggcgagcggg atgaggcgac cgtcggggcc
      241 ggtgcggctg gcccgccggg cgtggtgcag gagcatgagg gcgaggaggc cccgaacctc
      301 ggcgtcgtcc atcgccgccg ccagctggcg ggtgaggcgg atggcttcgg cggcaaggtc
      361 gacgtcgccg gagaagccct cgttgaacac gaggtagagg acccgcatga cggtggcggc
      421 gtcgccgggc tggtcgaccc cggcgtcggc gacggtgcgc ttggcccggc tgatgcgctg
      481 ggccatcgtc gcctcgggca cgaggtaggc ctcggcgatc tggcgggtgg tgaggccgcc
      541 gacggcccgc agcgtgagcg ccaccgcgga cgacggggtc aggtcggggt gggcgcagag
      601 gaagtagagg cgcagggtgt cgtcgacggt cggcaccgcg cccggcagcg gctcggcgtc
      661 gacccgcatc tcccgctcca gccgggccga cgacgaccgg gccatgtcga ggaacacccg
      721 ccacgacacg gtgacgagcc aggccttcgg gtcccggggc gggtcgtcgg accaggaccg
      781 cagcgcccgg atgagcgcct cttggaccgc gtcctcggcg gtggcgaagt cggcgccccg
      841 gccgacgagg accgacagga cctgcgggac gagcgcccgc aggtcctggt catcgatcgt
      901 cacccgacca tccgatcact cgtcgaacgt gggcggcgcg ctcatgatcg gtcggagctc
      961 gagccactcg tagatcggct cgccgccggg gccgggcgcc gccgacagct cgccggccag
     1021 ctcgaccgca cgctcgtagg agtcgacgtc gatgatgaac cagccggcga tcaggtcctt
     1081 cgtctcggcg aacggcccgt cgaccaccgg cggcttgccc tcgcgccccg cctgcaccca
     1141 gacgccgtcg ggcgacagcc cctcgccgcc gaccatctcg ccggtggcct cgagccgagc
     1201 ggcgtagtcc cgcatgaact ggaagtgggc ttccacctcg tcaggcgtcc actggtccat
     1261 cgacgcgaac tcgttggggg tcggggtggt ggtccggtag tgcttgagca tcaggtactt
     1321 ggccatgagg gttctccttg cttcgtgccg gccctggtgg ccggctccac ccctgggacg
     1381 gagccgaggt gacgtcctcg acatcgttgc ccgagaaatc cccgcccgat ccccgagccg
     1441 gtcccgttca cgccacccgg cgtggaacgt ggaggaagag cgaccgcatg cgaccatctt
     1501 cctccacgtt ctgccaccag cctccaagtt ggtggatcgg ggtcgccagg cgaccgggat
     1561 gctccaagtt ggaggctggt ggcgggaccg gtgtcggggc tactggtcgg acatgagggc
     1621 gatgagctgg tcgagggcgg cgttccagcc ctcgtcgaag cccatctcct ggtgctgctt
     1681 cacgccggcc gggtcctggt ggagggcgac ggcccggtag cgggtgccgc cgttcggcaa
     1741 ccgctcgagc tcgaggatgg cggtgagcgg caggtcgatg gtcttggggc ggaagccggg
     1801 gtccatggac tcggtccaca ccaggcgctc gttggggacg acctcgaggt agcagccgac
     1861 gctcgggaac tcctcgccct cgggcgagcg catcgtcatc gccatcgcac cgccagggcg
     1921 caggtccacc gaggcttcgg cgatggccca gggcgccggg gcgaaccact gctcgatcag
     1981 ctccggctcg gtccaggccc gccagaccag ctcgggggcg acgtcgatct cgcggaccag
     2041 ctccaggtcc agctcggggt tcagggtgtg cggggtgaag gtcatgtcgg ttgctccttg
     2101 aggttgaggt tgaggaggag cccgtcgagc tggtcgagct tgcgctccca gatgcggcgc
     2161 tgctcggcca gccagccgtc gacgagctcc aaggccgtgc gggcgatgcg gtaggtccgc
     2221 acccgtccct ccttggtgga cgtcaccagg cccgcccgct ccagcacgcc caggtgctgg
     2281 gtgaacgacg gcaacgccat ggcgaacggc tcggccagct ccgacgtgga ggcaggaccc
     2341 gagacgagcc gttcgaccac ctggcgacgg gtggggtcgg ccaaggcgtg gagcacctcg
     2401 tcgatcccgt ccgaacgctt aggcataaac ctaagtattg acgcggcgcc cgcgtttgtc
     2461 cagcgcgggc cgcgacgccg tgccaacctc ctgacggacg acggacgacg gacgacagac
     2521 ggacggacgg acggacggac gacggacgga cgacaccgac cgcgggaggt cgacgccatg
     2581 ggccgctact tcaccaacga cgagtacgag gcgcagttcg gccgcacgct ctacgcggcg
     2641 atggtcgact cggccgacct gggcgaagcc gccgtgaccg cggaccgcat cgtggcaggc
     2701 ggcgaggacg atgcggcctg gtaccgggag tggacggcta ccgccgaggg cgtggtcgcc
     2761 gacgccgagc ggtcccgagc cgccggcaac gacgtcgacg cccgcagcgc gctcctgcgg
     2821 gccgccg
//
LOCUS       DATMFS010000248         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_170013, whole genome shotgun sequence.
ACCESSION   DATMFS010000248 DATMFS010000000
VERSION     DATMFS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170013"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1828)
                     /locus_tag="VNQ33_04580"
     CDS             complement(71..1828)
                     /locus_tag="VNQ33_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97411.1"
                     /translation="MANERSRWTRSAVVALMAAAALLPAAAGSEPTGATRHDGRRAAA
                     PVEPRILVDLSVARPSGLAVADDGTLYLTEPGTGRVVRLTDSGTEVLLRLGSDQEPNG
                     LALTDDGTLVVAATSGIVTLGPDGTRHDLPVPGAGEIAAVDVDTSGVVYASDRTAKQV
                     VRVEPDGTASVVPFTGLGEVEGIDVDDAGTVSVLDATAHDVVQRTAAGVQSSVGVTGL
                     VSPVGIDITDGRMLISDAGTGHLLERDADGTVRDLATDLPSPREVALPAGGAAYVAVD
                     GTSGCRCPEAPGSVTRVPDGAAAGAIDLGDHSTIRSVAAAGAATALFTSTDGAPGYGP
                     DNPLRRIVGDGPAEDVPFAGDPGAVDAAPDGTAYVVQFEGSTSSVLRRSPDGSTGPVD
                     LPTEPGMDQVAGISVDEQGRLFVAMGSGYGSGRFRVVEPLAAGGPKVRYDSGGQSLTA
                     MAAAGGLLHLAVSDGPGVSLLRVDLGTGTVTDRGPLDAPPYAFDADAAGTLYVIGDEP
                     ADAPDIHVIDPAGAVTALPYRGQRQPNELSIGTDGTLYVVDAEIGLLALDGIGPVTAP
                     IPPTAGPAAPVPGAPALTG"
     gene            1901..>2644
                     /locus_tag="VNQ33_04585"
     CDS             1901..>2644
                     /locus_tag="VNQ33_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HWJ97412.1"
                     /translation="MVTHEPEITEPVELCLPGGKRLNPAAKGWSRQVLHTANLRGRWG
                     RTKRWDYWAVLAGDWSIAIVYADVDYLGIAEVWWCHLPTGRTGGRAANVPGARGIALP
                     DLPGTDALRYRSSKIEVALTDDAEGTTLVAWWKEDDGSRSSLNARVDLPAAHESLNVV
                     IPWSDTQFQYTSKHQARPAHGTLEVGGDVVRFGSASTADGVADDDESTELVRVPEAWG
                     VLDVGRGRWPYETRWNWGGGAGRSVDGSVV"
BASE COUNT          341 a          969 c          986 g          348 t
ORIGIN      
        1 cgctgacgcc gcgctggccg agaccgagga gcgttggctc gccctcggcg aggagctgga
       61 aggctgaccg ctacccggtg agcgcgggcg cgcccggcac gggtgccgcg ggtccagccg
      121 tgggcgggat cggtgcggtc accggaccga tgccgtcgag ggccagcagc ccgatctcgg
      181 catccaccac gtagagggtc ccgtcggtcc cgatcgacag ctcgttcggc tggcgctggc
      241 cccggtaggg gagggcggtg acggcgcctg ccggatcgat gacgtggatg tccggcgcat
      301 cggccggttc gtcgccgatg acgtagagcg ttcccgccgc atcggcgtcg aacgcgtatg
      361 gaggtgcgtc gagagggccg cgatcggtca ccgtgccggt gccgagatcg acccggagca
      421 gcgagacgcc cgggccgtcg ctcaccgcga ggtgcaacag cccgcccgct gctgccatcg
      481 cggtgaggct ctggccgccg ctgtcgtagc ggaccttcgg tcctcccgca gccaggggct
      541 cgaccacccg gaagcgaccc gacccgtagc cgctgcccat cgccacgaag agccggccct
      601 gctcgtcgac gctgatcccg gcgacctggt ccatgcccgg ctcggtgggc aggtcgaccg
      661 gaccggtgga cccgtccggg gaccggcgca ggaccgagct ggtgcttccc tcgaactgca
      721 ccacgtaggc ggtgccgtcg ggcgcggcgt cgaccgcacc ggggtcgcca gcgaacggca
      781 cgtcctcggc ggggccgtcg cccacgatcc gccggagggg gttgtccggc ccgtagcccg
      841 gcgcgccgtc cgtcgaggtg aagagcgccg tcgccgcgcc tgcggcggcg acggagcgga
      901 tggtggagtg gtcgcccagg tcgatcgcgc cggccgcagc gccgtcggga acccgggtca
      961 cgctgcccgg ggcttccggg cagcggcagc cgctggttcc gtcgacggcg acgtaggccg
     1021 ccccgccggc cggcagggcc acctcccgtg gcgacggcag gtccgtggcg aggtcgcgga
     1081 cggtgccgtc cgcgtcgcgc tcgagcaggt ggccggtgcc ggcatcgctg atgagcatcc
     1141 ggccgtcggt gatgtcgatg ccgacggggc tcacgaggcc ggtgacgccg acggaggact
     1201 gcaccccggc cgcggtgcgc tggacgacgt cgtgcgcggt ggcatcgagg accgagacgg
     1261 tgccggcgtc atccacatcg atgccctcga cctcgcccag acccgtgaac ggcaccaccg
     1321 aggcggtgcc gtcgggctcg actcgaacga cctgcttcgc cgtccggtcg ctggcgtaca
     1381 ccacccccga cgtgtcgacg tcgaccgccg cgatctcgcc ggcaccgggg accgggaggt
     1441 cgtgtcgggt gccgtcggga ccgagcgtca cgatcccgct ggtggcagcg acgacgaggg
     1501 tgccgtcgtc ggtgagcgcc aagccgttgg gctcctggtc ggagccgagg cgcagcagca
     1561 cctcggttcc cgagtcggtc agccgcacca cgcgtccggt ccccggctcc gtgaggtaca
     1621 gcgttccgtc gtcggccacc gcgaggccgc tcggccgcgc gaccgagagg tcgacgagga
     1681 tccggggctc caccggggct gccgcccgtc gcccgtcgtg gcgggtggcc cccgtcggtt
     1741 cgctccccgc cgcggccggc agcagcgctg ccgccgccat cagcgccacg accgctgacc
     1801 gggtccacct gctccgctcg ttcgccacga tggactccat tcgtcacggg tcggtgacaa
     1861 ggtacgccgc cgcgagaaca ccgtgcttga ctcacgagcg atggtcacgc acgaacccga
     1921 gatcaccgag cccgtcgagc tgtgcctgcc gggcggcaag cggctgaacc cggcggccaa
     1981 gggctggtcc cgccaggtcc tccacaccgc caacctgcgc ggccggtggg gccgtaccaa
     2041 gcggtgggac tactgggccg tcctcgccgg cgactggtcg atcgccatcg tgtacgccga
     2101 cgtcgactac ctcgggatcg ccgaggtctg gtggtgccac ctgccgaccg ggaggaccgg
     2161 tggccgggcc gccaacgtcc cgggcgctcg cggcatcgcc ctgcccgacc tccccggcac
     2221 ggacgcgctg cggtaccggt cgtcgaagat cgaggtcgcc ctcaccgacg atgccgaggg
     2281 caccacgctc gtcgcctggt ggaaggagga cgacgggagc cgcagctccc tcaacgcccg
     2341 tgtcgacctg ccggccgccc acgagtcgct caacgtcgtg atcccctggt ccgacaccca
     2401 gttccagtac acgtcgaagc accaggcccg tccggcccac ggcacgctcg aggtcggcgg
     2461 ggacgtggtg cgcttcggct cggcgtcgac ggctgacggg gtcgcggacg acgacgagtc
     2521 caccgagctc gtgcgggtgc cggaagcctg gggcgtcctc gacgtcgggc ggggccgctg
     2581 gccctacgag acccggtgga actggggcgg cggcgccgga cgttcggtcg acgggtccgt
     2641 cgtc
//
LOCUS       DATMFS010000249         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_288514, whole genome shotgun sequence.
ACCESSION   DATMFS010000249 DATMFS010000000
VERSION     DATMFS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288514"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VNQ33_04590"
     CDS             complement(<1..403)
                     /locus_tag="VNQ33_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97413.1"
                     /translation="MSRSDDQRVADILEAADELDLVVAVGRVAFSDSVLHIRAAERLL
                     EIIGEASTASPTSSKTPTPKCRGAMSRRSGYCSHTTTTASISTRSGRSRRNRCPNSTA
                     RFAQTDDHRSASAGPWQQFFSGVGVPPIAGMV"
     gene            complement(400..690)
                     /locus_tag="VNQ33_04595"
     CDS             complement(400..690)
                     /locus_tag="VNQ33_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWJ97414.1"
                     /translation="MGAHRDLVESKRDEIRAAVQRHRGRSVAVFGSVARGDETESSDI
                     DFLVEFEPGSSLFDLLHLSEELERLLGVPVDVVSAGGLKDRDDHIRREAVVL"
     gene            1509..1997
                     /locus_tag="VNQ33_04600"
     CDS             1509..1997
                     /locus_tag="VNQ33_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWJ97415.1"
                     /translation="MSTLIAIGYDDTTTALSAMDESYKLAGDLIIQPDAIAAIVRSED
                     GKFRTTTNQHEVGSGATWGMFWGFLFGILFFVPFLGMAMGAAFGALGGKLAKGAIDQN
                     FQNQIRDALQPGTSALFLIVEEITTDKAIEALSQYGGNVIKTSLSKEAEAEIQAHLHG
                     EA"
BASE COUNT          360 a          764 c          753 g          323 t
ORIGIN      
        1 gcaccatccc tgcgatcggg ggcacgccaa cgccgctaaa gaactgctgc cagggtccgg
       61 ccgaagcaga ccgatggtcg tcagtctgag cgaagcgcgc tgttgagttc gggcaccgat
      121 tccgacgcga tctgccagac ctggtcgaga tcgacgcggt ggtagtggtg tgcgagcagt
      181 atccggagcg ccgagacatc gcgccacggc acttcggggt gggcgtcttt gaactcgtcg
      241 gagacgcggt gctcgcctcg ccgatgatct ccaacagacg ttcggcggcg cggatgtgga
      301 ggacgctgtc gctgaacgcc acgcggccca cggcgaccac gagatcgagc tcgtcggccg
      361 cttcgaggat gtcggcgacg cgctggtcgt cgctccggct caaaggacga cggcctcgcg
      421 tcggatgtga tcgtcccggt ccttcaaccc gccagcggag accacatcga cgggcacgcc
      481 cagcaagcgc tccagctcct cgctcaggtg cagcagatcg aagagcgagc ttcccggctc
      541 gaactcgacc aggaagtcaa tatcgctgga ctccgtctcg tcgccacgag ccaccgaccc
      601 gaacaccgcg actgaacgcc cccggtgtcg ctggacagcc gcgcggatct catcccgctt
      661 cgactccacg aggtcccggt gcgcacccac gccggtaacg ctaacgccaa gaacacgttc
      721 cgagagacga cgatcgcctc ctcatgtggc cacaggttga tcaacccccg gcgccgcggc
      781 gacacgtggc gggtcgagac gtgctcgacc tcgaacccgg gcaggcggcc tcgcgcgacg
      841 cgtggggcat gccctgggag cgcggtccag tgggcgactc gtgagcggcg cgaccgagcg
      901 ggtgacggct accgggcgcc tcggtgtccc gatctctggc ggatggcgtt cggcggtgtg
      961 gaccggacgc gcatccgctg cggtggcgcg gtgtcgaggt ggcggtcgac ggcggcggcc
     1021 tcgtggaggc tgcgccgccc agtcgacccg ggagggcggg tgcccttcgg cgtgaggcgg
     1081 ccggaggtca cggcacgggg gttcttcgag gagcgagatg caggcatggg agtcctctcg
     1141 agatggcgag gggtgggtcg gtgcgaccgg gatctcctgc ccatggcgcc ggcaacgccg
     1201 tgccaggtgc acgagcggta cggcgatgag gtcctgggag gccggtggga ggggccgacg
     1261 gggcggtgcc actcgtcggc gggagcacgc tcgggcgcgc gacgcatcac aggaacatgc
     1321 ggagcaacct acggacacgc ctcgctggcg gtcaacggag ttgcgctccg tctctgactc
     1381 gccgccgagc gttcacccga ccgccaccgg gtggcccggg atcgtgcccg atgtcaccgc
     1441 tagggtcagc caccgcgcgt ggcggtggtg ccgccctgcg cccccaccac cccaaccaag
     1501 gagcaccgat gtccacgctc atcgcgatcg gctacgacga caccaccacg gccctctcgg
     1561 ccatggacga gtcctacaag ctcgccggcg acctcatcat ccagcccgat gccatcgccg
     1621 ccatcgtccg ctcggaggac ggcaagttcc gcaccaccac caaccagcac gaagtcggct
     1681 ccggtgccac ctggggcatg ttctggggct tcctcttcgg catcctcttc ttcgtgccgt
     1741 tcctcgggat ggcgatgggc gcggcgttcg gtgcactcgg cggcaagctc gccaagggcg
     1801 ccatcgacca gaacttccag aaccagatcc gcgatgccct ccagcccggc acgtcggccc
     1861 tcttcctgat cgtcgaggag atcaccaccg acaaggcgat cgaggcgctg tcgcagtacg
     1921 gcggcaacgt catcaagacc tcgctctcca aggaggccga ggccgagatc caggcgcacc
     1981 tccacggcga ggcgtgatcc gtcggtcctg atcgacgcgg tgcccgggcg gccggtccgg
     2041 gcaccgtcgg ttcgggggaa cggcttggtc cggacccgaa gcgaagtgct agacaagact
     2101 gctcgtcggc tccggcccac ggaccaaacc agctagcccc catcgtctag cggcctagga
     2161 caccaccctc tcaaggtggc ggcacgggtt cgaatcccgt
//
LOCUS       DATMFS010000250         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_9900, whole genome shotgun sequence.
ACCESSION   DATMFS010000250 DATMFS010000000
VERSION     DATMFS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9900"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..908)
                     /locus_tag="VNQ33_04605"
     CDS             complement(177..908)
                     /locus_tag="VNQ33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWJ97416.1"
                     /translation="MANVTYELADGTATITVDDGKANAITEALIGEIHAALDAAEADG
                     AVVLLAGRPGRFSGGFDLPTLTAFTSESADLLKAGFDLSHRLLSFPRPVVIACTGHAI
                     AMGSFLLLSGDHRIGIADAGHKIGATEVAIGMAMPWAAIELLRQRLTRADFDRAALLA
                     EVFTPETAVEAGFLDEVVLEDQLLIRARETAARLASLDAKAHHVTKQRARGAMLETLA
                     AAIQRDDDEFRAAIAQLAADAGTSA"
     gene            complement(969..1652)
                     /locus_tag="VNQ33_04610"
     CDS             complement(969..1652)
                     /locus_tag="VNQ33_04610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97417.1"
                     /translation="MTWDQQFARGVRTVVDPVVGTFNRRPPKPGRSPWNHNIAYHHVA
                     LEAAPSPCRRALDVGCGQGHLVEELAAVTDSVVGLEPDPQALARAADRLDHLDNVTLV
                     QADVLTAELPLAGFDLVTAVAVLHHLPLEAGLSRLADLVAPGGSLVVIGLAEDQGWRD
                     LARSAVAFPVANALVLKRGHTDVGAPITDPTETLDDIRAAAHRIVPGAKIRRRLLFRY
                     TLVWTRPAG"
     gene            1745..>2317
                     /locus_tag="VNQ33_04615"
     CDS             1745..>2317
                     /locus_tag="VNQ33_04615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWJ97418.1"
                     /translation="MTETTSDKSVVRVGLLGCGNVGASLVQLVEARGDEIAARTGTRL
                     EIARVAVRSLTRKRPVHLDDAVLTRDASEVVTDPSVDVVVEVIGGIEPARELILAAMA
                     AGKPVVTANKELLANHGAELYAAASEAGVDLLFEAAVAGGIPILRPLRESLAGEDVRR
                     IMGIVNGTTNFILTRMSEQGTGYGEALAEAQ"
BASE COUNT          315 a          771 c          902 g          329 t
ORIGIN      
        1 ggccgatggg aaggcccgcc tggtgatccg ccgcgagacc gaacaggacc tcctcgccac
       61 cgacgtcggc tgaacccggg cgacgtcggt ggaaccccgg ggacgtcggc ggaaccctgg
      121 tgaggtcgcc acggttcggg gacgtcggtg gcatcgaagg gtgccgagtt cgatgctcag
      181 gctgaggttc cggcgtcggc ggcgagctgg gcgatggcgg cgcggaactc gtcgtcgtct
      241 cgctggatgg cggcggcgag ggtctcgagc atggcgcctc gggcccgctg cttggtgacg
      301 tggtgggcct tggcatcgag cgacgccaag cgggcggcgg tctcacgggc ccggatgagc
      361 agctggtcct cgagcacgac ctcgtcgagg aagccggcct cgacggcggt ctccggcgtg
      421 aagacctcgg ccaacagggc ggcgcggtcg aagtcggcgc gggtgaggcg ctggcgcagc
      481 agctcgatcg cggcccacgg catcgccatg ccgatcgcca cctcggtcgc tccgatcttg
      541 tgcccggcgt cggcgatgcc gatgcggtgg tcgccgctga gcagcaggaa cgaccccatg
      601 gcgatggcgt ggccggtgca ggcgatcacg accggtcgcg ggaacgacag cagccggtgg
      661 gagaggtcga agccggcctt cagcagatcg gccgactcgg aggtgaacgc ggtcagcgtc
      721 ggcaggtcga acccgccgga gaagcggccg ggcctgccgg ccagcaacac gacggcgcca
      781 tcggcctcgg ccgcgtcgag cgcggcatgg atctcgccga tgagcgcctc ggtgatggcg
      841 ttggccttgc cgtcgtcgac ggtgatcgtg gcggtgccgt cggccagctc gtaggtcacg
      901 ttcgccatcg gtggcgtccc cttcgtggat gtcctgggtg gtctggcgac gacgctagcg
      961 aggcgcggtc agccagccgg gcgggtccac acgagcgtgt agcggaacag gaggcggcgc
     1021 cggatcttcg cccccgggac gatccggtgc gcggcggctc ggatgtcgtc gagcgtctcg
     1081 gtcgggtcgg tgatcggcgc gccgacgtcg gtgtggccgc gcttgagcac gagcgcgttg
     1141 gccaccggga aggcgacagc tgaccgggcc aggtctcgcc agccctggtc ctcggccagg
     1201 ccgatgacga cgagggagcc accgggtgcg accaggtcgg cgaggcggga gagcccggcc
     1261 tccagcggca ggtggtggag cacggcgacc gcggtgacca ggtcgaaccc cgcgaggggc
     1321 agttcggcgg tgagcacgtc ggcttgcacc agcgtgacgt tgtcgaggtg gtcgagccgg
     1381 tcggcagccc gggcaagggc ctgcgggtcg ggttcgaggc ccacgaccga gtcggtgacg
     1441 gcggcgagct cctcgaccag gtggccctgc ccgcacccga cgtcgagcgc tcggcgacag
     1501 ggcgatggtg cggcttccag cgctacgtgg tggtaggcga tgttgtggtt ccacggcgac
     1561 cgtcccggct tgggagggcg ccggttgaag gtgcccacca ccgggtcgac gacggtgcgg
     1621 acgcctcggg cgaactgctg atcccacgtc acggcgccac gttggcacac cgggtctgcg
     1681 cgtcgcggtc gcgcccggcc cttctcgttc ggggcggtga tcgggtggcc ggtagcctcg
     1741 ccccgtgacc gagacgacct ctgacaagtc cgtggtgcgc gtgggcctgc tcggctgcgg
     1801 caacgtgggc gcgtccctcg tgcagctggt ggaagctcgt ggcgacgaga tcgccgcccg
     1861 gaccggcacc cgcctggaga tcgcccgggt cgccgtgcgc agcctcaccc gcaagcgccc
     1921 cgtccacctc gatgacgcgg tcctcacccg ggatgcgtcc gaggtggtca ccgatccttc
     1981 ggtcgacgtc gtggtcgagg tcatcggcgg catcgagccg gcccgggagc tgatcctggc
     2041 ggccatggcg gcgggcaagc cggtcgtcac cgccaacaag gagctgctcg ccaaccacgg
     2101 cgccgagctc tacgcggcag catcggaagc cggcgtcgac ctcctgttcg aggcagccgt
     2161 cgccggtggc atcccgatcc tgcggcccct gcgggagtcg ctggcgggcg aggacgtgcg
     2221 ccgcatcatg ggcatcgtca acggcaccac gaacttcatc ctcacccgca tgtccgagca
     2281 gggcacgggg tacggcgagg cgctcgccga ggcccag
//
LOCUS       DATMFS010000251         3615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_32639, whole genome shotgun sequence.
ACCESSION   DATMFS010000251 DATMFS010000000
VERSION     DATMFS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32639"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VNQ33_04620"
     CDS             <1..515
                     /locus_tag="VNQ33_04620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ97419.1"
                     /translation="KQCIDLDKTYTATFETSKGDITVELDTKRTPVTTNNFVNLARSG
                     YYDGTDIHRVVESAGFVQGGSPHTQDAADPGPGYTIPDEGGPYTAEDYVPGILAMART
                     NEPNSAGAQFFFLAHDGGTDALASYGTYAVFGKTTKGLDVLAAMAALDDGTEAPTEQI
                     ILEHVTIEES"
     gene            complement(544..1209)
                     /locus_tag="VNQ33_04625"
     CDS             complement(544..1209)
                     /locus_tag="VNQ33_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HWJ97420.1"
                     /translation="MSGHLALVGGGEFTEGCTFDAGLLEASGASEVLIVPTAAAYEHP
                     QRLVERAAGWFDRLGVPARGLDLLARPDASAPAVVEALAASTFTYLVGGSPLHLRAVL
                     KDSPAWDAIEAAWLGGGVLAGSSAGAMVLCDPMVDPRGGAFTLGLGLRPGVAVIPAHN
                     HWSEDAAHRTRKMSPPDLVLAGIDESTALIRDPEGAWRAEGAGEVVLFLGGAPTDLSA
                     LPG"
     gene            1274..2131
                     /locus_tag="VNQ33_04630"
     CDS             1274..2131
                     /locus_tag="VNQ33_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWJ97421.1"
                     /translation="MTIKRVGILGSGIMGSGIAEVAAKTGHEVVLRSRKQETADAMVA
                     SLTKSLTKQVERGKLSQEDADAALARVTATDHIGAVADCDLVIESVVEDMDVKKALFA
                     ELDGIIKDGGIIATNTSTLSVIDMAVATQRPDRVVGVHFFNPAPMMSLVEIVRPLTAS
                     DETVATVTAFATACGKDPVEVKDRAGFIVNALLFPYLNNAVRMLENGTANRDDIDTAM
                     KGGCNFPMGPLALLDLVGLDTSLSILDALYEEFRDPNYAAVPALRRMVTAGQLGRKSG
                     IGFYDYRKN"
     gene            2261..2932
                     /gene="aat"
                     /locus_tag="VNQ33_04635"
     CDS             2261..2932
                     /gene="aat"
                     /locus_tag="VNQ33_04635"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWJ97422.1"
                     /translation="MSLPVEPPPSRWEMPDPDRADASGLVAIGADLEPGTILAAYRTG
                     LFPMPVRWSRAPGWWSPDPRGIIPLDGLKVSRSLRKACDRMEVRVDVAFDDVIEACSD
                     PLRPHGWISKGIKAAYRDLHRRGWAHSVEAWSDGELVGGLYGIAIGGLFAGESMFHRA
                     TDASKVALVGLVDRLNEGGAALLDVQWKTDHLATLGAIEIARAEYRERVAEAVRRPLP
                     PAFGG"
     gene            2977..3255
                     /locus_tag="VNQ33_04640"
     CDS             2977..3255
                     /locus_tag="VNQ33_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWJ97423.1"
                     /translation="MCRNITTLRGLEPHATHEEIEAAARQYVRKISGVQKTSDATEEA
                     FERAVAIITEASHDLLHALPPRKVPPKYDPPLRRIAAKKAAETAPAAS"
BASE COUNT          579 a         1345 c         1215 g          476 t
ORIGIN      
        1 cgaagcagtg catcgacctc gacaagacct acacggccac cttcgagacc tcgaagggcg
       61 acatcacggt cgaactcgac accaagcgga cgcccgtcac cacgaacaac ttcgtgaacc
      121 tcgcccgctc cgggtactac gacgggaccg acatccaccg cgtcgtcgag tccgccggct
      181 tcgtgcaggg cggctcgccc cacacccaag acgccgccga ccccgggccc ggctacacga
      241 tcccggatga aggcggcccg tacacggccg aggactacgt acccgggatc ttggcgatgg
      301 cgcggaccaa cgaacccaac agcgctggcg cccagttctt cttcctggcc catgacggcg
      361 gtaccgacgc gctcgccagc tacgggacct acgcggtgtt cggcaagacg accaagggcc
      421 tcgacgtcct cgcggccatg gccgccctcg atgacggcac cgaggccccc accgagcaga
      481 tcatcctcga gcacgtgacc atcgaggaga gctgagcgag cccgcctcca agcccacctg
      541 gcgctagccc gggagcgcgc tcaggtccgt gggggcgccg ccgaggaaca ggaccacctc
      601 accggcgccc tcggcacgcc aggccccctc cgggtcccgg atcaaggcgg tggactcgtc
      661 gatgccggcc aggaccaggt ccggtgggct catcttgcgg gtccggtgtg cagcgtcttc
      721 ggaccagtgg ttgtgagccg ggatcacggc gacccctggc cgcaggccga gcccgagggt
      781 gaacgcgccg ccccgggggt cgaccatcgg gtcgcacagg accatggccc cagcggacga
      841 accagcgagc acgccgcccc ccagccatgc cgcctcgatg gcatcccacg caggcgagtc
      901 cttgagcacc gcccggaggt gcaggggcga gcccccgacg aggtaggtga acgtcgacgc
      961 cgccagcgcc tccaccaccg cgggagccga ggcatcagga cgagcgagca ggtccaggcc
     1021 gcgggcgggc acgccgaggc ggtcgaacca gccggccgcc cgctcgacga ggcgctgggg
     1081 gtgctcgtag gccgcggcgg tgggcacgat gagcacctcg gaggcgccgc tggcctccag
     1141 cagacccgcg tcgaacgtgc atccctcggt gaactcgccg ccgccgacca gggccaggtg
     1201 tccagacatt cccctgaccc tatccaggcg ccggttgggc gccaggaagg ccggatcggt
     1261 agcttgaccg cccatgacga tcaagcgtgt gggcattctg ggttcgggga tcatggggtc
     1321 cggcatcgcc gaggtggcgg ccaagaccgg ccacgaggtg gtgctgcggt cccgcaagca
     1381 ggagacggcc gacgccatgg tcgcctccct caccaagtcc ctgaccaagc aggtcgagcg
     1441 gggcaagctc tcgcaggagg acgccgacgc cgccctggcc cgcgtcaccg ccaccgacca
     1501 catcggcgcc gtcgccgact gcgacctcgt gatcgagtcg gtcgtcgagg acatggacgt
     1561 gaagaaggcg ctgttcgctg agctcgacgg catcatcaaa gacggcggca tcatcgccac
     1621 caacacctcg acgctgtcgg tcatcgacat ggccgtcgcc acccagcgcc ccgaccgcgt
     1681 cgtcggcgtg cacttcttca acccggcccc gatgatgtcg ctcgtcgaga tcgtccggcc
     1741 gctcaccgcc tccgacgaga ccgtcgccac cgtcaccgcc ttcgccacgg cctgcggcaa
     1801 ggaccccgtc gaggtcaaag accgcgccgg cttcatcgtg aacgccctgc tgttccccta
     1861 cctcaacaac gccgtgcgca tgctcgagaa cggcaccgcc aaccgcgacg acatcgacac
     1921 ggcgatgaag ggcggctgca acttcccgat gggcccgctc gccctgctcg acctggtggg
     1981 cctcgacacc tcgctgtcga tcctcgacgc cctgtacgag gagttccgcg acccgaacta
     2041 cgccgccgtg cccgccctgc gccgcatggt caccgccggc cagctcggcc ggaagtccgg
     2101 catcggcttc tacgactacc ggaagaacta gcccccgtac cgaagttggt gtgcgaatcg
     2161 aggcagagcg tcgattcgca caccaacgtc gagcgcagcc gaaccgggtt ccgcaccgag
     2221 cgcgagccgt cgaccacggt tctgccaagc tcgacgggcc atgagcctgc ccgtcgagcc
     2281 gccgcccagc cggtgggaga tgcccgaccc ggatcgggcc gatgcgtcgg gtctggtcgc
     2341 catcggggcc gacctggaac cgggcacgat cctcgccgcg taccggaccg ggctgttccc
     2401 gatgccggtc cgctggagcc gggcgccggg atggtggtcg cccgatcccc gcgggatcat
     2461 cccgctcgac gggctgaagg tgagccgctc gctgcgcaag gcgtgcgacc ggatggaggt
     2521 gcgcgtcgac gtcgcgttcg acgacgtgat cgaggcgtgc agcgacccgc tccggccgca
     2581 cggctggatc agcaagggca tcaaggcggc gtaccgagac ctgcaccgcc ggggctgggc
     2641 gcacagcgtc gaggcctggt ccgacggcga actggtcggt gggctgtacg ggatcgcgat
     2701 cggaggcctg ttcgccggtg agtcgatgtt ccaccgggcg accgacgcgt cgaaggtggc
     2761 cctggtcggc ctggtcgacc ggctgaacga ggggggtgcc gccctgctcg acgtccagtg
     2821 gaagaccgac cacctggcca cgctcggcgc gatcgagatc gcccgggcgg agtaccggga
     2881 gcgcgtcgcc gaggcggtgc ggcggccgct cccgccggcg ttcggcgggt gaccgaccag
     2941 gtgacagcgg cagagggccg cgcgaccatg ggtcgcatgt gccgcaacat caccaccctc
     3001 cgggggctcg agccccacgc cacccacgag gagatcgagg cggcggcccg ccagtacgtg
     3061 cgcaagatca gtggcgtgca gaagacgtct gacgccaccg aggaggcgtt cgagcgcgcc
     3121 gtcgccatca tcaccgaggc atcgcacgac ctgctccacg ccctgccgcc ccgcaaggtc
     3181 ccgccgaagt acgacccccc gctccgccgc atcgccgcca aaaaggcggc cgagaccgca
     3241 cctgccgctt cctagccgag cccgtccggc ccgcctctga tgcgtggcgt ttggtgaccg
     3301 gtgcctgtgc acctgggcgc tcaccgccct ctccggacga cgtcatggtc gtttcgatgc
     3361 gagacctggc cgactcgctg gctgctctgg cccagcgcca cgggctctcg ggcctcggtg
     3421 ccgaggcggt ggcggcggtc gtcctcctcg acgcgccgtt gcgcgcctcc gacgatggcc
     3481 ctcgcatccg agccgcggcg gcccaggagc aggtcacctc tcgcgtggtc gatccccctg
     3541 aggtagggcc gcctcgtact gcatcgcgcc gatcacgttc cccgacgggt cttcgagcca
     3601 cgccagggtg ccgac
//
LOCUS       DATMFS010000252         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_240550, whole genome shotgun sequence.
ACCESSION   DATMFS010000252 DATMFS010000000
VERSION     DATMFS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240550"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VNQ33_04645"
     CDS             <1..207
                     /locus_tag="VNQ33_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97424.1"
                     /translation="DFAPRWIEAVVICAAALFGTISVAWVTQPMTDWPYERYRQLHGL
                     LRRGAGPSSATRPASSGTQPASAS"
     gene            complement(215..1114)
                     /locus_tag="VNQ33_04650"
     CDS             complement(215..1114)
                     /locus_tag="VNQ33_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ97425.1"
                     /translation="MTGDRSSSAPRLGICLSEFPQDADGRWRNVGEVVQRVEDSGADG
                     LWLADHILWHSKAIDAQSTLPALASHATSITIGSCVLQMPLRDPVTVAKTMANTQMMA
                     PGRVVCGLGIGENVEEYERLHKSMATRGKVIEASIDEIRRIWATEGGTRWMNPAPVDI
                     PIWVGGRAEAARRRAAQMGDGWLPYLCSPRWFGRHRTLMEEEAEAAGRDHAAITKAVV
                     AFVDIPAVESAQQGAEKLEHWFGFDPAPLMGFLLRGSADEVAQQLHEYVDQGASEVIV
                     VIANDRPLDVLDQLSPAFDALSA"
     gene            1149..1787
                     /locus_tag="VNQ33_04655"
     CDS             1149..1787
                     /locus_tag="VNQ33_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97426.1"
                     /translation="MVGIIMSRDAGIGQACADELGMALGSAAAVVQVHDAGGVASVDD
                     PVEVLVTVPAHPVPGVASRDDQAAFQLEVQRAVEAVLPGMVARGGGRIVLVVTATGLP
                     GQTWRDGTGSAMWAMIGLARSAAREVAAAGVTVNVVRTGLIDDEPSRAAAAADGTVAE
                     AITAAQRLAPVRRPVVPADVAAAVGYLVSPDASYVTGVVLPVDGGLTIGQGT"
     gene            complement(1833..>2066)
                     /locus_tag="VNQ33_04660"
     CDS             complement(1833..>2066)
                     /locus_tag="VNQ33_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97427.1"
                     /translation="GGLFRFDGTAPPADLLWLLGYAVVGVLLLDGRERRREAVAGHRD
                     PVTLFRAVAFGLMVVGIVVFSGPAPQSFLYFNF"
BASE COUNT          280 a          730 c          781 g          275 t
ORIGIN      
        1 gacttcgccc cccgctggat cgaggcggtc gtcatctgcg ccgctgccct cttcggcacc
       61 atctccgtgg cctgggtgac ccagcccatg accgactggc cctacgagcg ctaccgccag
      121 ctccacggtc tgctgcgccg cggcgccggt ccgtcgagcg ccacccgccc ggcttcatcc
      181 ggcacccagc ccgccagcgc cagctgacgg cccctcaggc actgagggcg tcgaaggcgg
      241 gggagagctg gtcgaggacg tcgagcgggc ggtcgttggc gatcacgacg atcacctcgg
      301 acgctccctg gtcgacgtac tcgtggagct gctgggcgac ctcgtccgcg ctgccccgga
      361 gcaggaagcc catgagcggt gccgggtcga agccgaacca gtgctcgagc ttctccgcgc
      421 cctgctgcgc gctctcgacc gccgggatgt cgacgaaggc caccacggcc ttggtgatgg
      481 ccgcatggtc gcggcccgca gcctcggcct cctcctccat cagggtccgg tgccggccga
      541 accagcgggg cgagcagagg tacggcagcc agccgtcgcc catctgagcg gcgcggcggc
      601 gggcggcctc ggcccggccc ccgacccaga tcgggatgtc gaccggcgcc gggttcatcc
      661 aacgggtgcc gccttcggtg gcccagatgc gccggatctc gtcgatcgac gcctcgatga
      721 ccttgccgcg cgtcgccatc gacttgtgga ggcgctcgta ctcctcgacg ttctcgccga
      781 tgcccagccc gcacacgacc cggcccggcg ccatcatctg ggtgttcgcc atcgtcttgg
      841 ccacggtcac gggatcgcgc agcggcatct ggagcacgca ggagccgatg gtgatcgagg
      901 tggcgtgcga ggccagggcc ggcagcgtcg actgggcgtc gatggccttg gagtgccaga
      961 ggatgtggtc ggcgagccac aggccgtcgg caccggagtc ctcgacgcgc tgcacgacct
     1021 cacccacgtt gcgccaccgg ccatcggcgt cctgggggaa ctccgagagg cagatgccga
     1081 ggcgaggagc ggaggaggag cgatcgccgg tcatctccgc aggctaggtc ggcgggtagc
     1141 gtgacctggt ggtcgggatc atcatgagcc gcgatgccgg catcggccag gcgtgcgcgg
     1201 atgagctggg gatggcgctg gggtcggcgg cggccgtggt ccaggtccac gacgcaggcg
     1261 gcgtcgcgag cgtcgacgat ccggtcgagg tgctcgtcac cgtgcccgcc cacccggtgc
     1321 ccggcgtggc gagccgcgac gaccaggcgg ccttccagct cgaggtccag cgggcggtgg
     1381 aggccgtgct gcccggcatg gtcgcccggg gcggcggccg gatcgtgctc gtggtcacgg
     1441 ccaccggcct gccgggtcag acgtggcgcg acggcacggg ctcggcgatg tgggcgatga
     1501 tcggcctcgc tcggagcgcg gcgcgcgagg tcgccgccgc cggggtcacc gtgaacgtgg
     1561 tccgcaccgg cctgatcgac gacgagccga gccgggcggc ggccgccgcc gatggtacgg
     1621 tggccgaagc catcacggcc gcgcagcggc tcgcacccgt gcgcaggccg gtggtgccag
     1681 ccgacgtcgc cgccgccgtg ggctacctgg tgtcgcccga cgcctcctac gtcaccggcg
     1741 tcgtcctccc ggtcgacggt ggcctcacga tcggccaggg cacgtgaacc gcgcgatcgc
     1801 gatcggcgcg ggcttcgtcg ggggcgcagc cgtcagaagt tgaagtacaa gaagctctgg
     1861 ggggcggggc cgctgaagac gacgatgccg acgaccatga ggccgaaggc gacggcgcga
     1921 aacagcgtga cggggtctcg gtggccggcc acggcctcgc ggcggcgctc gcggccgtcg
     1981 aggagcagca cgccgacgac ggcgtagccg aggagccaga gcaggtcagc cggcggcgcg
     2041 gtgccgtcga agcggaacaa gccgcc
//
LOCUS       DATMFS010000253         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_9994, whole genome shotgun sequence.
ACCESSION   DATMFS010000253 DATMFS010000000
VERSION     DATMFS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9994"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="VNQ33_04665"
     CDS             complement(<1..1989)
                     /locus_tag="VNQ33_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97428.1"
                     /translation="MDDLLTFTIIGLVTGSLYAIAASGLVVTYTTSGIFNFAHGATGM
                     FAAFVYWRLRVDLGWPAPIALVAVLFVIAPLFGAVVERVIIRGLWNVSEVTKLIVSIS
                     LLFGVYSAATILFSPNVGRRLPGFFEGSSIDLGVYDLSWHEAITIGAAALTAAVLRFL
                     LFGTRSGVAMRAVVDDRDLSRLNGGRPDRSAMLSWAIGSSLAALAGILLAGSQGALTH
                     IPLTLLVVNAYGAAMFGRLRSLPLTFLGAALIGLAESYAIGYLKLDTPLGSIVGHQLA
                     TPLSLNGFRAAIPVIILFVVLLFLPPLKVRAGAQLTSREIIPSPGAPRWLLGTLVLGA
                     AAFAFATMYEGIRTFQMGQGLALGIVMLSLVPLIGYAGQISLAPMAFAGIGAIVMGGW
                     GGGGSPLGLVAAVLVTAAVGALVALPAIRLRGLYLALATAAFAVLMDQLVFNQSAVMP
                     NGSLAVGRLDLFGFSFDDDRAHLVLIAVVFGLVSTLIVWMRDRPFGRRLLAMKSSDAA
                     CVTLGVNIASTKLQVFTLSAGIAGLGGALYGAQLNSVSPPTFQFLNGLPIVLFAVIGG
                     IAAVGGALFGGIVYALSFLILPEVWPALTNLLVLGPALAGISLGRNPNGAVNETARSI
                     RQYRAERAAAKEAAASGTTIDDLDLIGLDGFTDAERVEL"
     gene            complement(1996..>2541)
                     /locus_tag="VNQ33_04670"
     CDS             complement(1996..>2541)
                     /locus_tag="VNQ33_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ97429.1"
                     /translation="VTFQKFIGEGANAAAFEQARQQVGFEPEVRYYETNFYDQAFIDA
                     GGSAVDGALISSVFIPMEEAADHPATQQYLDVMDQQGGKKALLGMQSTSAWLLFATLA
                     RDCDQAGDLTRSCILKGADAVTEWTGGGLHAATDPGENDPAECQMVMEIKDGKFTRWG
                     PTDEDFSCPDDAVVEVEPATK"
BASE COUNT          427 a          852 c          936 g          326 t
ORIGIN      
        1 gagctcgacg cgctcggcgt cggtgaagcc gtcgaggccg atcaggtcca ggtcgtcgat
       61 cgtggtcccc gacgctgcgg cctccttggc cgccgcccgc tcggcgcggt actggcggat
      121 cgagcgggcg gtctcgttga cggcgccgtt cgggttgcgc ccgaggctga tgccggcgag
      181 ggccggaccg agcacgagca ggttcgtgag cgccggccac acctcgggga ggatcaggaa
      241 gctgagggcg tagacgatgc cgccgaacag cgcgccgccc acggcggcga tgccgccgat
      301 gacggcgaac aacacgatgg gcaggccgtt caggaactgg aaggtgggcg ggctcaccga
      361 gttgagctgg gcgccgtaga gcgcaccgcc gagcccggcg atgcccgccg acagggtgaa
      421 gacctggagc ttggtgctgg cgatgttcac cccgagcgtg acgcatgcgg catcgctgga
      481 cttcatggcg agcagccgcc ggccgaaggg ccggtctcgc atccacacga tgagggtgga
      541 caccaggccg aagacgacgg cgatgagcac caggtgcgct cggtcgtcgt cgaacgagaa
      601 cccgaacagg tcgaggcgcc ccacggccag gctgccgttc ggcatcaccg cgctctggtt
      661 gaacacgagc tggtccatga gcaccgcgaa cgccgcggtg gccagggcga ggtagaggcc
      721 gcggaggcgg atcgccggca gggccaccag ggcaccgacc gcggcggtca ccaagacggc
      781 ggccacgagg ccgagcggcg atccgccgcc gccccacccg cccatcacga tggcgccgat
      841 gccggcgaac gccatcggtg ccagcgagat ctggccggcg tagccgatga gcggcacgag
      901 cgacagcatc acgatgccga gcgccaggcc ctggcccatc tggaacgtgc ggatgccctc
      961 gtacatcgtg gcgaaggcga aggccgcggc gccgaggacg agcgtgccca gcagccaccg
     1021 gggggcaccg ggtgacggga tgatctcgcg ggaggtgagc tgcgcccccg cccgcacctt
     1081 cagcggtggg aggaagagca gcaccacgaa caagatgatc accgggatgg cggctcggaa
     1141 cccgttgagc gagagcggcg tcgccagctg gtgcccgacg atcgagccga gcggggtgtc
     1201 gagcttgagg tacccgatgg cgtaggactc ggcgaggccg atgagggcgg cgccgaggaa
     1261 cgtgagcggc aggctgcgga ggcgcccgaa catcgccgcg ccgtaggcgt tgaccaccag
     1321 cagggtcagc gggatgtggg tgagcgcccc ctggctcccg gcgagcagga tcccggccag
     1381 cgcggcgagc gacgacccga tggcccacga cagcatcgcc gagcgatcgg ggcggccgcc
     1441 gttgagccgc gacaggtcgc ggtcgtcgac gaccgcccgc atcgccaccc cgctccgggt
     1501 cccgaacagg aggaagcgca gcacggcggc ggtgagggcg gcggccccga tggtgatggc
     1561 ttcgtgccag gacaggtcgt acacgccgag gtcgatggag ctcccctcga agaacccggg
     1621 gaggcgccgg cccacgttcg gcgagaacag gatcgtcgcc gccgagtaga cgccgaagag
     1681 gaggctgatc gacacgatga gcttcgtcac ctcggacacg ttccacagcc cgcggatgat
     1741 cacccgctcc accaccgcgc cgaacagcgg cgcgatgacg aacagcaccg cgacgagcgc
     1801 gatcggtgcc ggccacccca ggtcgacgcg cagcctccag tacacgaacg ccgcgaacat
     1861 gccggtggcg ccgtgggcga agttgaagat ccccgacgtc gtgtaggtga ccaccaaccc
     1921 gctcgccgcg atcgcgtaga gcgagccggt gaccagcccg atgatcgtga acgtgaggag
     1981 gtcgtccaag gcctgctact tcgtggccgg ctcgacctcg accaccgcgt cgtcggggca
     2041 cgagaagtcc tcgtcggtcg ggccccagcg ggtgaacttc ccgtctttga tctccatgac
     2101 catctggcat tcggccgggt cgttctcccc ggggtcggtg gcggcgtgga gcccgcctcc
     2161 ggtccactcg gtgacggcgt cggcgccctt caggatgcag ctccgggtga ggtcgccggc
     2221 ctggtcgcag tcgcgggcca aggtggcgaa cagcagccac gccgaggtgc tctgcatgcc
     2281 gagcagcgcc ttcttgccgc cctgttggtc catcacgtcg aggtactgct gggtggccgg
     2341 gtggtccgcc gcctcctcca tggggatgaa cacgctgctg atcaaggcgc cgtcgacggc
     2401 cgaaccgccg gcgtcgatga aggcctggtc gtagaagttc gtctcgtagt accggacctc
     2461 gggctcgaaa ccgacctgct ggcgggcctg ctcgaaggcg gcggcgttgg cgccctcgcc
     2521 gatgaacttc tggaacgtca c
//
LOCUS       DATMFS010000254         4926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_10000, whole genome shotgun sequence.
ACCESSION   DATMFS010000254 DATMFS010000000
VERSION     DATMFS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10000"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VNQ33_04675"
     CDS             complement(<1..370)
                     /locus_tag="VNQ33_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWJ97430.1"
                     /translation="MPDFSPFRSLRYDPARFAPEDVTAPPYDVLSPEDRAALVAKNPH
                     NAVLIDLPTEDDGDDRYAEAGRRLQAWIDEGALVRDGAPELTAYRMTTTDDLGNPVAT
                     LGVLGSLALSRPDEGEILPHE"
     gene            483..941
                     /locus_tag="VNQ33_04680"
     CDS             483..941
                     /locus_tag="VNQ33_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97431.1"
                     /translation="MAETNVLKKYLDAGVSFTNLTQAKAEALVKELVKVGEVQTEQAQ
                     AAVLDLLERSKKNTEALIEQIRKEISESAESLGLATIADITRLEKLIASIKIPGTGSA
                     APEAPAPAAKKAPAKKAPAKKKAAAKKAPAKKAAAKKAPAKKAAAKKSAS"
     gene            886..1758
                     /locus_tag="VNQ33_04685"
     CDS             886..1758
                     /locus_tag="VNQ33_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWJ97432.1"
                     /translation="MRRRRPRPRRPPPRSRPAEAPTATRRRLDAELVRRGLIPSRERA
                     REAIERGEVVVAGAPATKASRLVAADEPIVVSGPPARFVGRGGEKLAGALDRFDVDLA
                     DARVLDAGASTGGFTDCALQAGASRVIAVDVGRGQLHQRLRVDPRVEVRERTNVRHLA
                     PGDLGPAVDAVVADLSFISLRTVTPALLGLVRDGGDLIWLVKPQFEAGRQEASRNRGV
                     IADPAVWHRVLGEVSGTLREHGAAIMGLMTSPLRGADGNVEFLLHARAASSQPLGVDV
                     EGSIDAVVAEAGAT"
     gene            1755..2591
                     /locus_tag="VNQ33_04690"
     CDS             1755..2591
                     /locus_tag="VNQ33_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWJ97433.1"
                     /translation="MSTVGLFVHSGRDDAIALGRTVAAWLAHRGHTVVLEPGEAELIG
                     ATGAVLDDDRAELDLVVAIGGDGTVLRAARRAVAADADVLGINLGELGYLAEIEPDAW
                     EAALEAYLAGGHQLEERMLVSARTESGALPPGLNEVVIEKQALGRTIRLGVSIDGGYF
                     TSYVADGLIVATPTGSTAYSLSARGPIVAPSHRALVMTPVSPHTLFDRSLVLGPTSIV
                     EVAVLGDRDAAVSIDGYEQGRLAVGESISITAAPDPARFATFGGRNFHQILKAKFGLS
                     DR"
     gene            2588..4240
                     /gene="recN"
                     /locus_tag="VNQ33_04695"
     CDS             2588..4240
                     /gene="recN"
                     /locus_tag="VNQ33_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWJ97434.1"
                     /translation="MSGTAPPPDHLVELAVTDFGIIEHVRLLFGPGMTALTGETGAGK
                     TLLIGAIDLLLGGRADPALVRTGRDEAVVEGRFVVDGEEVVLARVVPTDGRSRAYVNG
                     RMATATVLGEQARALIDLHGQHAHQSLLAPAVQRAALDQFASVDPEPLRAARAERRRV
                     AASLEELGGDAGARAREADLLRFQISEIEAASLADAEEDAALDELEDRLADAVAHREA
                     ASRAVEALGGDGAAVDQVGAAVADLDGRSPFAGLLERLRSLQAELTDVAAELRSTGEA
                     IEDDPARLAEIRERRQLLVELRRKYGSAPHGGSPEGGAGTLADVIAYGDDALQRLADI
                     DGHDARAAQLDREGAAAVEAEARAAAAVGRARRGAAADLAAAVEANLAELAMASARFT
                     VEVGETDPGDDVTFTLAANPGSAPGPLAKVASGGELARAMLALRLVLSVAPPVLVFDE
                     VDAGIGGGAALAVGRSLAALGADHQVLVVTHLPQVAAFADAQVAVRKELTDGITVARA
                     RPLTPDDRVVELSRMLSGTPESDRVREAADELLHLAARERGR"
     gene            4360..>4926
                     /gene="steA"
                     /locus_tag="VNQ33_04700"
     CDS             4360..>4926
                     /gene="steA"
                     /locus_tag="VNQ33_04700"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HWJ97435.1"
                     /translation="MTDHEHDHDATSAVLSDRAEHAGDAHTAWPSITGIARVDRRTKD
                     LAKRLVPGEIAVIDHTDLDTVAAESLLASGVVGVVNASRCISGRYPNPGPLLLSAAGV
                     VVVDGMGEDIMTAVADGTVVTIEGDRVLVDGQVTASGTRQDTATFGLVLDHARNNMGD
                     ELQRFAENTLNYIQREGHLLIDDPDIPDI"
BASE COUNT          681 a         1812 c         1794 g          639 t
ORIGIN      
        1 gctcgtgagg gaggatctcg ccctcgtccg ggcggctgag cgccaaggac ccgagcacgc
       61 ccaaggtggc gaccgggttc ccgaggtcgt cggtggtggt catccggtac gccgtcagct
      121 ccggcgcgcc gtcgcgcacc agcgcgcctt cgtcgatcca cgcctgcagc cggcgcccgg
      181 cctcggcgta gcggtcgtcg ccgtcgtctt cggtcgggag gtcgatcagg accgcgttgt
      241 gcgggttctt ggcgacgagc gccgcccggt cctcggggga gaggacgtcg tagggcggag
      301 cggtcacgtc ctcgggggcg aaccgggccg ggtcgtagcg gagggagcgg aacggggaga
      361 agtcgggcac cgctctttgc tagcaggcgc gtccgacgcc gctgtgagcc ttcggtggcc
      421 tctggacgat cagggtgcta actcttgcaa gcagaacgct cgcgggacta tcgtttggct
      481 ccatggctga gaccaacgtc ctcaagaagt acctcgatgc cggggtgtcc ttcaccaacc
      541 tgacccaggc gaaggcggaa gccctcgtca aggagctggt caaggttggc gaagtccaga
      601 ccgagcaggc ccaggccgcc gtcttggatc tcctcgagcg gagcaagaag aacaccgagg
      661 cgctcatcga gcagatccgc aaggagatct cggagtcggc ggagagcctc gggctcgcca
      721 ccatcgccga catcacccgg ctcgagaagc tgatcgcctc catcaagatc cccggtaccg
      781 gctcggccgc tcccgaggct ccggctcccg ccgccaagaa ggccccggcc aagaaggccc
      841 ccgccaagaa gaaggccgca gcgaagaagg cgcccgccaa gaaggctgcg gcgaagaagg
      901 ccccggccaa gaaggccgcc gccaagaagt cggccagctg aggctcccac ggccacgcgc
      961 cgcaggctcg acgccgaact ggtccgtcga ggcctgatcc ccagcagaga acgggcccgc
     1021 gaggccatcg agcgaggcga ggtcgtggtg gccggggcac cggcgaccaa ggcgtcgcgc
     1081 ctcgtcgcgg ccgatgagcc catcgtcgtc tccgggccgc cggcccggtt cgtcggccgg
     1141 ggcggggaga agctcgcggg ggccctcgac cgcttcgacg tcgatctggc cgatgcccgg
     1201 gtgctcgacg ccggcgcgtc caccggtggc ttcacggact gcgcgctcca ggccggagcc
     1261 agccgggtca tcgccgtcga cgtcgggcgc gggcagctgc accagcggct ccgggtcgat
     1321 ccccgggtcg aggtgcgcga gcgcaccaac gtgcgccacc tcgcgccggg cgacctcggc
     1381 cccgcggtcg acgccgtggt cgccgacctg agcttcatct cgctgcggac cgtcacgccg
     1441 gccctgctcg gcttggtgcg cgacggcggc gatctcatct ggctggtgaa gccgcagttc
     1501 gaagccggcc ggcaagaggc gtcccgcaac cgaggggtca tcgccgaccc ggcggtgtgg
     1561 caccgcgtcc tgggtgaggt ctctggcacg ctccgggagc acggagccgc catcatgggg
     1621 ctgatgacct ctccgctccg tggtgccgat ggcaacgtcg agttcctctt gcacgcccgg
     1681 gccgcctcgt cccagcccct cggcgtcgac gtcgagggct cgatcgatgc ggtggtggct
     1741 gaggccgggg cgacgtgagc accgtcgggc tcttcgtcca cagcggccgc gacgacgcca
     1801 tcgccctcgg tcgcaccgtc gcggcctggc tcgcccaccg cgggcacacc gtcgtgctgg
     1861 agcccggcga ggctgagctg atcggcgcga cgggtgcggt gctcgacgat gaccgagccg
     1921 agctggatct cgtcgtggcg atcggcggcg acggcaccgt cctgcgggcc gcgcgacggg
     1981 cggtcgcggc cgacgccgac gtcctcggca tcaacctggg cgagctcggc tacctcgccg
     2041 agatcgagcc cgatgcctgg gaggccgccc tcgaggccta cctcgccggc gggcaccagc
     2101 tcgaagagcg catgctcgtg tccgcccgga ccgagtccgg cgcgctgccc cccggcctca
     2161 acgaggtcgt catcgagaag caggccctgg gccgcacgat ccggctgggg gtgagcatcg
     2221 acggcggcta cttcacgagc tacgtggccg acggtctcat cgtggccacc cccaccggct
     2281 cgacggcgta ctcgctgtcg gctcggggcc ccatcgtggc gccgtcgcac cgggccctgg
     2341 tgatgacgcc ggtgtcgccg cacaccctct tcgaccgctc gctggtgctc ggccccacca
     2401 gcatcgtcga ggtggccgtg ctgggagacc gcgacgctgc cgtgtccatc gacggctacg
     2461 agcagggccg gctggcggtg ggcgagtcca tcagcatcac ggccgcgccg gaccccgcgc
     2521 gcttcgcgac cttcggcggg cggaacttcc accagatcct caaggccaag ttcgggctga
     2581 gcgaccgatg agcggtaccg cgccgccgcc cgaccacctg gtcgagctgg ccgtcaccga
     2641 cttcgggatc atcgagcacg tgcggctcct cttcggaccg gggatgacgg ccctgacggg
     2701 ggagacgggc gccggcaaga cgctgctgat cggcgcgatc gacctgctgc tcggcggccg
     2761 cgccgatcca gcgctggtcc gcaccgggcg cgacgaagcg gtggtcgagg ggcggttcgt
     2821 ggtcgacggc gaggaggtcg tcctcgcacg cgtcgtgccc accgacgggc ggtcgcgggc
     2881 ctacgtcaac gggcgcatgg ccacggccac ggtgctgggc gagcaggcgc gggcgctgat
     2941 cgacctccac ggccagcacg cccaccagtc gctgctggcc ccggcggtcc agcgggcggc
     3001 gctcgaccag ttcgcctcgg tcgaccccga gcccctccga gcagctcggg ccgagcgtcg
     3061 ccgcgtcgcc gcatccctcg aggagctcgg gggagatgcc ggtgcccggg cgcgggaggc
     3121 cgatctcctg cggttccaga tcagcgagat cgaagccgcc tcgctcgccg acgccgagga
     3181 ggacgcggcg ctcgacgagc tggaggaccg cctggccgac gccgtggcgc accgggaggc
     3241 cgcgtcccgg gccgtcgagg ccctcggtgg cgacggcgcg gccgtcgacc aggtgggtgc
     3301 ggcggtggcg gacctcgacg ggcggtcgcc gttcgccggc ctcctcgagc ggctccgctc
     3361 cctgcaggcc gagctcaccg acgtggcggc ggagctgcgc tccaccggtg aggcgatcga
     3421 ggacgacccc gcccggctgg cggagatccg cgagcgccgc cagctgctgg tggagctccg
     3481 ccgcaagtac ggcagcgcgc cgcacggcgg aagcccagag ggcggcgccg gcacgctcgc
     3541 cgacgtcatc gcctacggcg atgacgcgct ccagcgcctg gcggacatcg acggccatga
     3601 cgcccgggcg gcgcagctcg accgcgaggg tgctgcggcc gtggaggccg aggcccgagc
     3661 cgccgcggcc gtcggccggg ctcgccgtgg cgccgcggcc gacctggccg cggcggtgga
     3721 ggccaacctc gcggagctgg ccatggccag cgcccgcttc acggtcgagg tcggcgagac
     3781 cgacccgggc gacgacgtga ccttcacgct ggccgccaac cccggttcag cccccggccc
     3841 gctggcgaag gtggcctcgg gcggcgagct ggctcgggcc atgctcgccc tgcggctcgt
     3901 gttgagcgtc gccccgccgg tcctggtgtt cgacgaggtc gacgccggca tcggcggcgg
     3961 cgccgccctg gccgtcggcc gatcgctcgc cgccctgggc gccgaccacc aggtcctggt
     4021 ggtcacccac ctcccgcagg tggcggcgtt cgccgatgcg caggtggccg tccgcaagga
     4081 gctcaccgac gggatcaccg tcgcccgcgc ccggccgctc acccccgatg accgggtggt
     4141 ggagctgtcg cggatgctgt cgggcacacc ggagagcgat cgcgtgcgcg aggcggccga
     4201 cgagctgctc cacctcgcgg cgcgggaacg ggggcggtga gccctgcctg gtccgttcac
     4261 cgagacgtca cctatgctcg ccgcggcgac gcgcggtccc ccaggcgacg gcaccgccac
     4321 tcgtccgcac cagacggtgc ccgaccgccg gggtgggtga tgacagacca cgagcacgat
     4381 cacgacgcca cgagtgcggt tctgtccgac cgagccgagc acgccggcga cgcccacacc
     4441 gcctggccgt ccatcaccgg gatcgcccga gtcgaccgga ggacgaagga cctcgccaag
     4501 cggctcgtgc ccggggagat cgcggtgatc gaccacaccg acctcgacac cgtcgccgcg
     4561 gagagcctgc tcgcctcggg tgtcgtcggc gtggtcaacg cgtcgcggtg catctcgggc
     4621 cgctatccca accccggtcc gctcctgctc agcgccgcag gggtggtggt cgtcgacggc
     4681 atgggcgagg acatcatgac cgccgtcgcc gacgggacgg tcgtcaccat cgagggcgac
     4741 cgggtgctgg tcgacggcca ggtcaccgcc tcgggcaccc gccaagacac cgccaccttc
     4801 ggtctcgtgc tcgatcacgc ccgcaacaac atgggcgacg agctccagcg cttcgccgag
     4861 aacaccctca actacatcca gcgcgagggc cacctcctga tcgacgaccc cgacatcccg
     4921 gacatt
//
LOCUS       DATMFS010000255         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_335691, whole genome shotgun sequence.
ACCESSION   DATMFS010000255 DATMFS010000000
VERSION     DATMFS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335691"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..490)
                     /locus_tag="VNQ33_04705"
     CDS             complement(44..490)
                     /locus_tag="VNQ33_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97436.1"
                     /translation="MRAKPGPLALVVIVAVAVAVAVAVTALVVGRSVLRDDPPCAALR
                     QPVAGLDGYDWDNGVTSSGSAWISVLADGTTEADAPSRRQIADAVEVDDAGFDRFRAA
                     LPGELEAVADRQRSVAVDPESNRADPAVERDARALARHGMATCNLV"
     gene            complement(511..999)
                     /locus_tag="VNQ33_04710"
     CDS             complement(511..999)
                     /locus_tag="VNQ33_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWJ97437.1"
                     /translation="MPAFDVDGPIGKLTRKMATSSWFRKVGPRVVPPMDRTLHKLTGG
                     RVIVSKMMVPSMVLTTTGVKSGLPRESPLACVPDGEGGWWVVGSNFGQEKHPAWTGNL
                     IADPNASVSYEHQDTPVLSHLLTDEEKAETWPKLLAVWPAYDDYVASSGRNLRVFHLT
                     PA"
     gene            1020..1814
                     /locus_tag="VNQ33_04715"
     CDS             1020..1814
                     /locus_tag="VNQ33_04715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97438.1"
                     /translation="MSTKAFPWAGKHVVITGGSSGIGEALAVALAVRGTKLTLLARGA
                     DRLAAVAGPLGATGVPCDVSDRDAVAGVLAAAAEERGPVDVLVCSAGITLPGRFLEAE
                     DQTFRDMIDVDYYGTLWPVRAVAPSMAERQTGSIVGVASAAALVGVYGYAAYGAAKFA
                     VRGLFETLRTELVPHGVHVGCVFPPDVDTPMLAAENLVKPAETKAIAGSIKPIAADQV
                     AQAVIRGVEKRQAEIYADAQTRALARTVATAPGIYRTLFDRRVRTA"
     gene            complement(1843..>2158)
                     /locus_tag="VNQ33_04720"
     CDS             complement(1843..>2158)
                     /locus_tag="VNQ33_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97439.1"
                     /translation="LPVKVVATKHDKVKPSKRDQRRRDLAEGCLLTPADVLWVSSAKN
                     VNIDELRARIMGWLAPVEDWSPAPPTRKERAEARAAEAAAASVPAPNDEYDEYDDEDE
                     WA"
BASE COUNT          286 a          776 c          789 g          307 t
ORIGIN      
        1 gaggcgcgtc acgggtcagg ctggtcgagg ggaaggggcg ggctcagacg aggttgcagg
       61 tcgccatgcc gtgacgggcc agggcgcggg catcgcgctc gaccgccgga tccgctcgat
      121 tcgactcggg gtcgaccgcg acggagcgct ggcggtcggc taccgcctcc agttcgccgg
      181 gcagcgcggc gcggaagcgg tcgaagccgg cgtcgtccac ctccaccgcg tcggcgatct
      241 gccgacggct cggtgcgtca gcctccgtgg tgccgtcggc caggacgctg atccacgccg
      301 agcccgacga cgtgacgccg ttgtcccagt cgtagccgtc gagaccggcg accggctggc
      361 ggagggctgc gcagggtgga tcgtctcgga gcacgctgcg accgacgacc agggcggtca
      421 ccgccaccgc caccgccacc gccaccgcca cgatcaccac cagcgccagg ggtcccggct
      481 tcgcccgcat cgcccgaccg taccggccga tcaggccggg gtcaggtgga agacccgcag
      541 gttcctgccg ctggatgcca cgtagtcgtc gtaggcgggc cagacggcga ggagcttggg
      601 ccaggtctcg gccttctcct cgtcggtgag gaggtgcgac aggacgggtg tgtcctggtg
      661 ctcgtacgag acgctggcgt tggggtcggc gatgaggttg ccggtccagg ccggatgctt
      721 ctcctggccg aagttggagc cgaccaccca ccagccgccc tcaccgtcgg gcacgcacgc
      781 cagcggcgac tcgcggggca ggccggactt cacgcccgtc gtcgtgagca ccatgctcgg
      841 caccatcatc ttgctgacga tgacccggcc gccggtgagc ttgtggaggg tgcggtccat
      901 cggcggcacg accctcgggc cgaccttccg gaaccacgag ctggtcgcca tcttgcgggt
      961 gagcttcccg atcgggccat cgacgtcgaa cgcaggcatg gggtgagagg ctacgaccca
     1021 tgagcacgaa ggcgttcccc tgggccggca agcacgtcgt catcaccggc ggatcgagcg
     1081 gcatcggcga agcgctggcg gtcgccctgg ccgtcagggg cacgaagctg acgctgctgg
     1141 cccgcggtgc cgaccgcctc gccgcggtgg ccggtccgct cggggcgacc ggggtgccgt
     1201 gcgacgtgag cgaccgcgac gcggtggccg gcgtgctggc cgccgcggcc gaggagcgag
     1261 ggccggtcga cgtactggtc tgctcggccg gcatcaccct gccgggccgg ttcctcgagg
     1321 ccgaggacca gacgttccgg gacatgatcg acgtcgacta ctacggcacg ctctggccgg
     1381 tgcgggcggt agctccgtcg atggccgagc ggcagaccgg gtcgatcgtc ggcgtggcgt
     1441 cggccgccgc cctcgtcggc gtctacgggt acgccgcgta cggcgccgcc aagttcgcgg
     1501 tgcgcggcct gttcgagacg ctgcggaccg agctggtccc ccacggggtc cacgtcggct
     1561 gcgtgttccc gcccgacgtc gacacgccga tgctcgccgc cgagaacctg gtgaaaccgg
     1621 ccgagaccaa ggccatcgcc ggctccatca agcccatcgc cgccgaccag gtggcccagg
     1681 cggtcatccg cggcgtcgag aagcgccagg ccgagatcta cgccgacgcc cagacgaggg
     1741 ccctcgcccg caccgtggcc acggcccccg ggatctaccg caccctcttc gaccgacggg
     1801 tccgcacggc ctgacggccg gcggcgtcag gccgtccgac cgtcaggccc actcgtcttc
     1861 gtcgtcgtac tcgtcgtact cgtcgttcgg cgccgggacc gaggcggcag cagcctccgc
     1921 ggccctcgcc tcggctcgct ccttgcgggt gggcggggcg ggcgaccagt cctccaccgg
     1981 ggcgagccag cccatgatgc gggcgcggag ctcgtcgatg ttcacgttct tggccgagct
     2041 gacccagagg acgtcggccg gcgtgagcag gcagccctcg gccagatccc tgcggcgctg
     2101 gtcgcgcttc gacggcttca ccttgtcgtg cttggtggcc acgaccttga ccggcagc
//
LOCUS       DATMFS010000256         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_263834, whole genome shotgun sequence.
ACCESSION   DATMFS010000256 DATMFS010000000
VERSION     DATMFS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263834"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..593)
                     /locus_tag="VNQ33_04725"
     CDS             complement(9..593)
                     /locus_tag="VNQ33_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97440.1"
                     /translation="MLFPKALWAGLEAGEVTVAFRSWKRPTVKTGGTLITPAGRLAID
                     AVEVIDPIAITDADVRQAGAVDADAQRALLRTEDGRRTYRVAFHLLGDDPRIALRNDA
                     ALSDDERAAIRTKLAGYDRRSPTGPWTTAALEVIRDHPAVVSTELAERLGTERPAFKL
                     NVRKLKALGLTESLDVGYRLSPRGEAYLAGGGAG"
     gene            complement(595..1116)
                     /locus_tag="VNQ33_04730"
     CDS             complement(595..1116)
                     /locus_tag="VNQ33_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97441.1"
                     /translation="MIGCYPGSFNPPTVAHLAVAQAAVDAAGLDRLDLVVSRVALGKE
                     HLPEATLAERVGVLESVAASRPWLRVAVTDARLITDIARSYDAVVMGADKWRQVNDAA
                     WYADEAERDAAVASLPLVLVAPRGDDRPDGVRLLDLDDAHRHVSATAVRDGAEHARGW
                     VLPEAADQHRSER"
     gene            complement(1113..1553)
                     /locus_tag="VNQ33_04735"
     CDS             complement(1113..1553)
                     /locus_tag="VNQ33_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HWJ97442.1"
                     /translation="MSAGYSGTPLAKKLGIEEGHVVALLSAPAGFDAVLAETLPDAVA
                     VRTRATGTADVIVTFHTSRAQLERRIDALLRVLDVDGGLWIGWPKRASGVATDITEDT
                     VREVFLPLGLVDNKVCAIDGTWSGLRVVWRKELRAERRAMRATS"
     gene            1649..>2427
                     /locus_tag="VNQ33_04740"
     CDS             1649..>2427
                     /locus_tag="VNQ33_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ97443.1"
                     /translation="MSRMQVVIVGAGFGGLAAARELADAPVDVTLVDRNNFHTFQPLL
                     YQVATSGLASADVAYPVRGIAARQENLAFRQADVIGADLEAKVLHLQHPEHEVEDLVY
                     DHLVVAAGATTNTFGIPGVDHHGFPLYNLADAVRLRNHVLERFEAADAEPRLVDDGAL
                     TFVVVGGGPTGVEVAGALTELFHVVLRRDFRTLDVGRARVVLVEMLPELLGPFSTVSR
                     AHAREALEERGVEVRTGAEVARVRATRVTLADGEELAAHTL"
BASE COUNT          311 a          835 c          944 g          337 t
ORIGIN      
        1 gggatcgttc agccggcgcc cccgcccgcc aggtaggcct cgccccgagg cgacagccgg
       61 tagccgacgt ccaggctctc ggtgaggccg agggccttca gcttgcggac gttgagcttg
      121 aacgcgggcc gctcggtgcc gaggcgctcg gcgagctcgg tcgacaccac cgcggggtgg
      181 tcccggatca cctcgagagc cgccgtggtc cacgggccgg tcggggagcg gcggtcgtag
      241 ccggcgagct tggtgcggat cgccgcccgc tcgtcgtcgg agagcgccgc gtcgttgcgc
      301 agggcgatgc gcgggtcgtc gcccagcagg tggaaggcca cccggtacgt ccggcggccg
      361 tcttcggtcc ggagcagggc ccgctgggca tcagcatcca ccgcgccggc ctggcggacg
      421 tcggcatcgg tgatcgcgat ggggtcgatg acctcgacgg cgtcgatcgc caaccgaccc
      481 gccggggtga tgagcgtgcc gccggtcttc accgtcggac gcttccacga ccggaacgcc
      541 acggtcacct cgccggcctc gagcccggcc cacagggctt tggggaagag cacgtcagcg
      601 ctctgatcgg tgctggtcgg cggcctcggg caggacccac ccgcgtgcgt gctcggcgcc
      661 gtcgcgcacg gcggtcgcgc tcacgtggcg gtgcgcgtcg tcgaggtcga gcagccggac
      721 gccgtcaggt cggtcgtcgc cgcgcggcgc gaccaggacg agggggaggg aggcgacggc
      781 cgcatcgcgc tcggcttcgt cggcgtacca ggcggcgtcg ttgacctgcc gccacttgtc
      841 ggcgcccatg acgacggcgt cgtaggagcg ggcgatgtcg gtgatgaggc gggcatcggt
      901 gacggcgacc cggagccacg gccggctggc ggcgacggac tcgagcaccc cgacgcgctc
      961 ggcgagcgtg gcttcgggga ggtgctcctt gccgagcgcg acccggctca cgacgaggtc
     1021 gagccggtcg agcccggcgg cgtcgaccgc ggcttgggcc accgcgaggt gggccacggt
     1081 cggggggttg aaggagccgg ggtagcagcc gatcacgacg tggccctcat cgctcgacgt
     1141 tcagcccgga gctccttgcg ccagacgacc cggagcccgg accaggtgcc gtcgatggcg
     1201 cagaccttgt tgtcgacgag gcccagcggg aggaacacct cgcgcaccgt gtcctcggtg
     1261 atgtcggtcg ccacgccgct ggcgcgcttg ggccagccga tccagaggcc gccgtcgacg
     1321 tcgagcacgc ggaggagcgc gtcgatgcgg cgctcgagct gcgctcgcga ggtgtggaac
     1381 gtgacgatga cgtcggcggt ccccgtggca cgggtgcgga cggcgacggc gtcggggagc
     1441 gtctcggcga gcacggcgtc gaagccggcc ggggcgctga ggagcgcgac gacgtggccc
     1501 tcctcgatgc ccagcttctt ggccagcggc gttcccgagt agcccgcgct catcggccca
     1561 gtgtgccggt tgggccggcg accgggccgg catgcggctc ttgtgaaaag aagcacaatg
     1621 tcgagggcgc gatccgtagg ctgaggtcat gagccggatg caggtggtga tcgtgggggc
     1681 ggggttcggc gggctcgcgg ccgcccgcga gctggccgac gcgccggtcg acgtgaccct
     1741 ggtcgaccgg aacaacttcc acacgttcca gccgctgctg taccaggtgg ccacctcggg
     1801 gctcgccagc gccgacgtcg cctacccggt gcgggggatc gcagcccgtc aggagaacct
     1861 ggcgttccgc caggccgacg tgatcggcgc cgacctcgaa gccaaggtgc tgcacctcca
     1921 gcaccccgag cacgaggtcg aggacctcgt ctacgaccac ctggtcgtcg cggctggcgc
     1981 caccaccaac accttcggga tcccgggcgt cgaccaccac ggcttcccgc tctacaacct
     2041 ggcggacgcg gtgcggctgc ggaaccatgt gctcgagcgc ttcgaggccg ctgacgcgga
     2101 gccgcgcctc gtcgacgacg gggcgctgac gttcgtcgtc gtgggcggcg gtcccacggg
     2161 cgtcgaggtc gcgggcgcgc tcaccgagct gttccacgtg gtgctgcgcc gggacttccg
     2221 cacgctcgac gtgggccggg cccgggtcgt cctcgtcgag atgctgccgg agctgttggg
     2281 tccgttcagc accgtgtcgc gggctcacgc ccgtgaggcg ctggaggagc gcggcgtcga
     2341 ggtccggacg ggtgcggagg tggcccgggt ccgggcgacc cgggtgacgc tcgccgacgg
     2401 cgaggagctg gcggcccaca cgctcat
//
LOCUS       DATMFS010000257         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_79296, whole genome shotgun sequence.
ACCESSION   DATMFS010000257 DATMFS010000000
VERSION     DATMFS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79296"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VNQ33_04745"
     CDS             <1..964
                     /locus_tag="VNQ33_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97444.1"
                     /translation="PPQFVVASFDGAGWHEKWEFWRAVQEAVPFHFTGFLSGTYLLSE
                     ATKDTYVGPGHDPGISSIAWAPPEAVPLVIADLNEALARGDEIGSHFNGHFCSGVARP
                     VGSWSTADWNDEMDQFDSFITNVGANNDIEDQLDLTPEDIKGERTPCLEGDPTQMFPA
                     FQQHGIVYDSSVTRTGLRWPVKDERYGIWQMGMSTFPMHGGGRPLITMDYNFYYRQRG
                     ASAGGVTPEESAADSEQVLGTYRDMYHAALNGNRAPLILGNHFNEWNNSAYQNAMADF
                     IEETCGEPETYCVPFEDLVAWMEAQDPAYVTDLQALPAENAPRP"
     gene            complement(1001..1387)
                     /locus_tag="VNQ33_04750"
     CDS             complement(1001..1387)
                     /locus_tag="VNQ33_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97445.1"
                     /translation="MITSVHSLIYSDDAPATRAFFRDVLGLPFVEDAGSEEGWLIFGT
                     GPSELGVHPTSTFWEGKQYDSPRQHVLSFMCDDLEATVAELTAKGATFASGPHEQSFG
                     VSAMVEVPGADPIMIYEPRHEIAYRL"
     gene            complement(1798..>2842)
                     /locus_tag="VNQ33_04755"
     CDS             complement(1798..>2842)
                     /locus_tag="VNQ33_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97446.1"
                     /translation="DERRVPATGWNRTMQPGSARGRGGARNRITAVLTYARPYTGSAG
                     TSTNELPAGSMTLVERDSGDAVPIMAGWPRSVPYGADAGEHMVDIQPAGDLKPCTWYR
                     AGVTANLVDARNQPVTPMTWDFRTGTDGKGNRCEDDPYNANENFVRKVTRDLFDRSAT
                     EQELQAANDGFERGTTRRTFTSNTLASAEERRVLVTAAFQQYLGRTPDPSGLNYWTTK
                     LKTITVPDLAARLLASDEVYRKAGSTNSGYVTALYPLVHGRDVDPSGHAYWTKKLDGG
                     LDRAKLAKMLLTSHESARRTVAQSYQDLLGRAPDPAGWDYWTAKFEKAGDPRTLWLSL
                     ITSNEYDRKAQAA"
BASE COUNT          403 a          966 c         1029 g          444 t
ORIGIN      
        1 gcccccgcag ttcgtggtgg ccagcttcga cggggccggc tggcacgaga agtgggagtt
       61 ctggcgcgcc gtccaagagg cggtcccgtt ccacttcacc ggcttcttgt ctggcaccta
      121 cctgctgagc gaggccacga aggacacgta cgtcggcccc ggccacgatc ccggcatctc
      181 gtcgatcgcc tgggcgcctc ccgaagccgt gccgctcgtg atcgcggacc tcaacgaggc
      241 cctcgcccgc ggcgacgaga tcggcagcca cttcaacggc cacttctgct ctggcgtcgc
      301 ccgtccggtc ggctcgtgga gcaccgcgga ctggaacgac gagatggacc agttcgactc
      361 gttcatcacc aacgtcggcg ccaacaacga catcgaagac cagctcgacc tcacgcccga
      421 ggacatcaag ggcgagcgga caccgtgcct cgagggcgac cccacccaga tgttcccggc
      481 gttccagcag cacgggatcg tctacgacag ctcggtcacc cgcaccggcc tgcggtggcc
      541 ggtgaaagat gagcgatacg gcatctggca gatgggcatg tcgacgtttc cgatgcacgg
      601 gggcggccgg ccgctgatca cgatggacta caacttctac taccgccagc gcggcgccag
      661 cgccggaggc gtgacgcccg aggagtcggc cgccgactcg gagcaggtcc tcggcaccta
      721 ccgcgacatg taccacgcgg cgctgaacgg caaccgggcc ccgttgatcc tgggcaacca
      781 cttcaacgag tggaacaact cggcgtacca gaacgccatg gccgacttca tcgaggagac
      841 gtgcggcgag cccgagacgt actgcgtgcc gttcgaggac ctcgtcgcct ggatggaagc
      901 ccaggatccg gcctacgtca ccgacctcca ggccctgccg gccgagaacg cacctcgccc
      961 gtaggcgccg ccgacgcctc cttgcggagc ccgctcgtgc tcagagccgg tacgcgatct
     1021 cgtgccgcgg ctcgtagatc atgatcggat ccgcaccggg cacctccacc atcgccgaca
     1081 ccccgaagct ctgctcgtgc gggcctgagg cgaacgtggc gcccttggcc gtgagctcgg
     1141 ccacggtcgc ctcgaggtcg tcgcacatga aggacaagac gtgctggcgg gggctgtcgt
     1201 actgcttgcc ctcccagaac gtggaggtcg ggtggacccc gagctcgctc ggcccggtgc
     1261 cgaagatcag ccacccctct tcggagccgg cgtcctccac gaagggcagg ccgagcacgt
     1321 cgcggaagaa ggcccgggtg gccggcgcgt cgtcggagta gatgaggctg tggaccgacg
     1381 tgatcatggg ccgcacccta ggacgctcgc ttcgcgggga cggggtgacg ggtacgtgga
     1441 ccgtgcggat cggaccggtg aactcatctc gaagcgcggg gggaccggcg tggtgagggg
     1501 tccgtccggg tagatcaaga gcacaccccc gccagaggag gccgatgtgc tcacgccgcc
     1561 tgcccatccg ttcgactcgc tccgcgtact gcggatcgtg gccgccacgg cgctcgccgc
     1621 cgcgctcggc tggtcgccgt tgcaccgctg accaggcgtc gccggctgga tcagctggcg
     1681 cggtgatcgc cacggcggcg ggccgatggt ccgcccggtg cgcggcggcg cgccgcccgg
     1741 caccgggccg ggcagcatcg ggcgacgtgc aggttccgcg cgccggttgg tcggcggtca
     1801 ggcggcttgg gccttgcggt cgtactcgtt gctcgtgatc agcgacagcc acagcgtgcg
     1861 ggggtcgccg gccttctcga acttggcggt ccagtagtcc cagcccgcag ggtcgggtgc
     1921 tcggccgagc aggtcctggt acgactgggc gaccgtccgg cgggccgact cgtggctggt
     1981 cagcagcatc ttggcgagct tggcgcggtc gaggccgccg tcgagcttct tcgtccagta
     2041 ggcgtggccg ctcgggtcga cgtcacggcc gtgcacgagc gggtacaggg cggtcacgta
     2101 gccgctgttg gtggagcccg ccttccggta cacctcgtcg gaggcgagga gccgagcagc
     2161 gaggtccggg accgtgatgg tcttcagctt ggtcgtccag tagttcaggc cgctcgggtc
     2221 cggggtccgg cccaggtact gctggaaggc agcggtgacc aggacccggc gctcttcggc
     2281 ggaggcgagg gtgttcgagg tgaaggtgcg gcgggtggtg ccccgctcga acccgtcgtt
     2341 cgcggcttgc agctcctgct cggtggcgga gcggtcgaac aggtccctgg tgaccttgcg
     2401 cacgaagttc tcgttggcgt tgtacgggtc atcctcgcag cggttgccct tgccgtcggt
     2461 gccggtgcgg aagtcccacg tcatcggcgt caccggctgg ttgcgggcgt cgaccaggtt
     2521 ggcggtgacg ccggcccggt accaggtgca gggcttcaga tccccggccg gctggatgtc
     2581 gaccatgtgc tcgccggcgt cggcgccgta cgggacgctc cggggccagc cggccatgat
     2641 cgggaccgcg tcacccgaat cgcgctcgac gagggtcatc gacccggccg ggagctcgtt
     2701 ggtcgaggtg ccggcgctgc ccgtgtaggg gcgggcgtag gtgagcaccg cggtgatgcg
     2761 gttccgcgcg ccgccgcggc cccgagcgga tccgggctgc atggtgcggt tccacccggt
     2821 ggcggggacg cgccgctcgt cg
//
LOCUS       DATMFS010000258         3843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_263963, whole genome shotgun sequence.
ACCESSION   DATMFS010000258 DATMFS010000000
VERSION     DATMFS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263963"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..695)
                     /locus_tag="VNQ33_04760"
     CDS             complement(108..695)
                     /locus_tag="VNQ33_04760"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWJ97447.1"
                     /translation="MGRRVLVIDNYDSFVYNLVQYLGELGAEPLVYRHDAITLEQAVD
                     LDPDGILISPGPGTPDDAGVSNEAIVHFTGKKPVFGVCLGHQCLGQVFGGTVVRAPEI
                     MHGKTSLIRHQGVGVFTGLPQPLEATRYHSLVVDRDSVPDVLEITAETDDGIVMGLRH
                     RDAPTEGVQFHPESILTVGGHDLVGNFLTQVDAYS"
     gene            complement(749..937)
                     /locus_tag="VNQ33_04765"
     CDS             complement(749..937)
                     /locus_tag="VNQ33_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97448.1"
                     /translation="MFAAAVIAVSFTGILVVTWLASRAEEAKTRCDEHRRRIERQLEI
                     PPVRPMAPRRPPRPPAPG"
     gene            1056..1655
                     /locus_tag="VNQ33_04770"
     CDS             1056..1655
                     /locus_tag="VNQ33_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HWJ97449.1"
                     /translation="MSEDSDETLADETSAGTEVDDAVDAPEPETSDAASGAAADETAD
                     ADADADGGAEEPDTAGDETAAAKADRKAKADAAVAERKVVARRTVTSRRVTPKGGPAA
                     AKAAKDTKGAAPVSTSKARDAKKTANVTKAPAPTPQQAYAQGPSPWYVPAIMFGLLII
                     GALIIMTNYMGVFGDASNIRLVLGLAFILGGIVTATQYR"
     gene            complement(1853..2158)
                     /locus_tag="VNQ33_04775"
     CDS             complement(1853..2158)
                     /locus_tag="VNQ33_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97450.1"
                     /translation="MSSAATPALVLGAFGGASVLRIVVAVAFAAVLLWMGSKMLGGFA
                     RPVPAPPDPGELRKVRFTFRCSICGTEVRMTQANDEVPEPPRHCMEDMDLVTSPYDE"
     gene            complement(2331..3524)
                     /locus_tag="VNQ33_04780"
     CDS             complement(2331..3524)
                     /locus_tag="VNQ33_04780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWJ97451.1"
                     /translation="MATAVIVDAVRTPGGKRYGKLSGWHAADLAAETLKALAERNDLD
                     PALVEDVIMGCVMQVGNQSVNVGRSAVLAAGWPEEVPATTIDRQCGSSQQSVHFAAQG
                     VIAGAYDIVVAAGVEVMSTTPMGASVTPGSFPMGPKVMERYAPRGGVVMQGTSAELIA
                     QKWGISREDMDAFAVESHKRAAAATAEGRFENEIIPVAEKRFDRETSQVIETGELVTA
                     DEGIRPDSSLESLAKLKPAFDPENGQVTAANSSQITDGASAVLIMSEDKAKELGLKPR
                     AKVHSFAVVGVDPILMLTGPIPATKKVLDKAGLTLDDIDLIEINEAFASVVLAWEKEY
                     SPDMSKVNVNGGAIALGHPLGCSGTKLMATLLNELERTGGRYGLQTMCEGAGMANATI
                     IERLD"
BASE COUNT          597 a         1287 c         1422 g          537 t
ORIGIN      
        1 ccacgacgac gctgccgccc acctccacga cgacctcgat cccctgagct gctcgtctcc
       61 gggcgtcggg tcggccggct ggtgagggcc ggcgcggcag gggccggtca ggagtaggcg
      121 tcgacctggg tgaggaagtt gcccacgagg tcgtgcccgc ccacggtcag gatcgactcg
      181 gggtggaact ggacgccctc ggtgggggcg tcgcggtggc gcaggcccat cacgatgccg
      241 tcgtcggtct cggcggtgat ctccagcacg tcgggcaccg agtcccggtc gaccaccagc
      301 gagtggtagc gggtggcctc gagcggctgc ggcagcccgg tgaacacgcc cacgccctgg
      361 tggcggatga gcgaggtctt gccgtgcatg atctccggcg cccgcaccac ggtgccgccg
      421 aagacctggc cgaggcactg gtggccgagg cagaccccga acaccggctt cttgcccgtg
      481 aagtggacga tggcctcgtt cgacacaccg gcgtcgtcgg gcgtgcccgg gccgggggag
      541 atcaagatcc cgtcaggatc gaggtcgacc gcctgctcga gggtgatggc gtcgtggcgg
      601 tacaccagcg gctcggcgcc cagctcaccc aggtactgga cgaggttgta gacgaaggag
      661 tcgtagttgt cgatcaccag cacgcggcgg cccatgggcg gccacggtag cgggggcatc
      721 gcgccggctc cgcctccgaa acgggcgctc agcccggcgc tggcggacgg ggtgggcggc
      781 gcggtgccat cgggcggacc ggggggatct ccagctggcg ctcgatgcgg cggcggtgct
      841 cgtcgcagcg ggtcttggcc tcctcggcac gggaggcgag ccacgtcacg accaagatgc
      901 cggtgaacga cacggcgatc acggcagcag cgaacatcag cggtcccttc gtcgatcgac
      961 gggtacccca ccgagggccg atcccaaccg cgacgtgacg gcggtgcccc ggtcgccgcc
     1021 agccgtccga cagcggttca gtaggctgcc ggaccatgtc cgaagactcc gacgagaccc
     1081 tcgcagacga aacctccgcc ggcaccgagg tggacgacgc cgtcgacgct cccgagcccg
     1141 agaccagcga tgcggcatct ggcgctgctg ctgacgagac cgccgatgcc gatgccgacg
     1201 ccgacggtgg cgcagaggag cccgacacgg ccggcgacga gaccgcagcg gccaaggcgg
     1261 accgcaaggc gaaggccgat gcggccgtgg ccgagcgcaa ggtcgtggcc cgccgcaccg
     1321 tcaccagccg ccgcgtgacc cccaagggcg gtccggcggc ggcgaaggcg gccaaagaca
     1381 ccaagggcgc ggcgccggtc tcgacctcga aggcccgtga cgccaagaag acggcgaacg
     1441 tgaccaaggc cccagcgccg acccctcagc aggcctacgc ccagggcccg agtccgtggt
     1501 acgtgccggc gatcatgttc ggcctgctca tcatcggtgc gctgatcatc atgaccaact
     1561 acatgggcgt gttcggcgac gcgtcgaaca tccgcctggt cctcggcctc gccttcatcc
     1621 tcggcggcat cgtcaccgcc acccagtacc gctaggccga gggcggagcg tcgccctgcc
     1681 gcggcgcccg cggcctcacc tggaatcgga catcgaggtt gccgtttgcg aacgggctgc
     1741 ggacagaggt tgccgggaag ttacagggat gtgaccctcc ccaatctttt ccacagcctg
     1801 gggaggacgg gcgccgtcgt ccccagggca cctcggggcc gtgaggcgga cgtcactcgt
     1861 cgtagggcga cgtgaccagg tccatgtcct ccatgcagtg acggggaggc tcggggacct
     1921 cgtcgttggc ctgggtcatg cgcacctcgg tgccgcagat gctgcaccgg aacgtgaagc
     1981 ggaccttgcg cagctcgccg gggtcgggcg gtgcgggaac ggggcgggcg aagccgccga
     2041 gcatcttcga ccccatccac aagaggacag cagcgaaggc cacggcgacg acgatgcgca
     2101 agacgctggc gccgccgaag gccccgagga cgagggcagg cgtggcggcg gaggacatgc
     2161 ggtcagccta cggacgcgac cgagggctca ccgaacccgg tcaccaccga ccggccccca
     2221 aaagcgacgc agccccggcc gagggccggg gctgcgcgcg atcagctcga acccggaggg
     2281 aggggtgggt tcggccgacc gctctggtga gcgtgacgtg ctggagctcg tcagtccagt
     2341 cgctcgatga tggtggcgtt ggccatgccg gcgccctcgc acatcgtctg gaggccgtag
     2401 cgaccgccgg tgcgctcgag ctcgttgagc agggtggcca tgagcttggt gcccgagcag
     2461 ccgagcgggt ggccgagggc gatggcgccg ccgttgacgt tcaccttgct catgtcgggg
     2521 gagtactcct tctcccaggc caagacgacg gaggcgaagg cctcgttgat ctcgatgagg
     2581 tcgatgtcgt cgagggtgag gccggccttg tcgagcacct tcttggtggc ggggatgggg
     2641 ccggtgagca tcaggatcgg gtcgacgccg acgacggcga acgagtgcac cttggcacgg
     2701 ggcttgaggc ccagctcctt ggccttgtct tcgctcatga tgagcacggc cgaggcgcca
     2761 tcggtgatct gggaggagtt ggccgcggtg acctggccgt tctccggatc gaacgcgggc
     2821 ttgagcttgg cgagggactc cagggaggag tcgggccgga tgccctcgtc ggcggtgacg
     2881 agctcgccgg tctcgatgac ctggctggtc tcgcggtcga agcgcttctc ggcgacgggg
     2941 atgatctcgt tctcgaaccg gccctcggcg gtggccgccg ccgcccgctt gtggctctcg
     3001 acggcgaagg cgtccatgtc ctcgcgggag atgccccact tctgggcgat gagctcggcc
     3061 gacgtgccct gcatgacgac gccgccgcgc ggggcgtagc gctccatgac cttggggccc
     3121 atggggaagc tgccgggggt gaccgaggca cccatcggcg tggtggacat gacctcgacg
     3181 ccggcggcga cgacgatgtc gtaggccccg gcgatgacgc cctgggcggc gaagtggacc
     3241 gactgctgcg aggagccgca ctggcggtcg atggtggtgg caggcacctc ctcgggccag
     3301 ccggcggcga gcacagccga gcggccgacg ttcacggact ggttgccgac ctgcatgacg
     3361 cagcccatga tcacgtcctc gacgagggcc ggatcgaggt cgttgcgctc ggcgagcgcc
     3421 ttgagcgtct cggcggccag gtcggcggcg tgccaacccg acagcttccc gtagcgcttg
     3481 ccgccgggcg tgcggacggc atcgacgatg acagcggtag ccatggtggc cccttcccct
     3541 tccggcccct gcggggtccg agtggcgttc ttgaccgggc ggtcaaggac cagtcaagcg
     3601 gggtctggtc ggggaacgca accccacccc gggtccgcgg gcgagccgag cgtcacaagc
     3661 gtctgctagt gaagggtccg acttcgacca ccgagccaga ggatcccgga aggccgccat
     3721 gacctccatc accgaacgca ccgccagcaa gaccatcgcc agcgccttcc tcgacgtggt
     3781 cgcgtcgcag ggaagcgacg tcgccgtccg ctggaaggag ggcgacgact ggaaggcgtg
     3841 gac
//
LOCUS       DATMFS010000259         3795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_311833, whole genome shotgun sequence.
ACCESSION   DATMFS010000259 DATMFS010000000
VERSION     DATMFS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311833"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VNQ33_04785"
     CDS             complement(<1..840)
                     /locus_tag="VNQ33_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWJ97452.1"
                     /translation="MTTTLLLLGLGISGLALLWAGWMARVRSREVQLADLLRLPYGEQ
                     DVDVARVAAEYGTVVSGTLGLAERAIKQAGSATSLRTRIERADLSVRPSELVVIAACS
                     GFAVGAAATAWTGRWWAVVLVVLVTPAVTSKVLDLLARRRNKRFAQQLPDALVLISSS
                     LSAGHTFLRSIQLLATEAESPMSDELRRVVSETQLGTSLVDSLEHLSTRMRNRDLEWM
                     VQAIRIQQQTGGHLTDLMATLAEVIRGREEVRREIAVLTAEGRISAWILGLMPVGLFL
                     AVET"
     gene            complement(837..2249)
                     /locus_tag="VNQ33_04790"
     CDS             complement(837..2249)
                     /locus_tag="VNQ33_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWJ97453.1"
                     /translation="MSLADRLAAVPDDVPGEPAPRRRPTPGPDDQWTATKRAVRAMVL
                     EEVAPIASDLGPDDLNAAVRSALDLILQREDVTVSPMERRAFVNEMIADTLGYGPLDP
                     LLQDESVTEIMCNGYDEVWVERSGRIEEAGVSFADEGQYRKVIERIVLAVGRRVDESS
                     PMVDARLSDGSRVNAVIPPLAINGPVLTIRKFAADPLTAMDLIATGTYTSDLVTVLDA
                     CVRGRMNILVSGGTGTGKTTNLNVMSSFVPASERIITIEDSAELQLQQRHVVSLEARP
                     ANAEGQGEVVIRDLVRNALRMRPDRIIIGECRSAEALDMLQAMNTGHEGSMTTVHANS
                     PRDAMSRLEMMVLMAGFDLPTRVVREQITAAVDIVVQCERLPNGRRLVTSLSEITGLE
                     GDTIVMQEVFRFDVSGSDDVGQLLATGLRPAFADRLATKGFPLPASVFRAPAAGPAPR
                     RPIEVPDASTLAGRTIGAER"
     gene            complement(2246..3589)
                     /locus_tag="VNQ33_04795"
     CDS             complement(2246..3589)
                     /locus_tag="VNQ33_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97454.1"
                     /translation="MSSVNGSAPPTAEVSEPPPAEAVQTAVVQTAPVVLVHDQRGALW
                     SKVDAVAATLAPRPVVLRSADLGELGRLLTEQHVDVLVAGPGLDNRSGIERLRIIRDE
                     LPRLVTVLAVDRPDHHDAATVVRAGAVDLVQSTIDEERLAATINRAASLALRIVDPAP
                     SAAEPLPAPPPERPQRVITVASASGGCGKTFLATNLAWFLTHKGGRRVCILDLDLQFG
                     EVTAALQLRPRYTITDLIQHGDEPQSELDAHIEEFCEQDETGVWVLAAPRDPTEAAMI
                     QPEDIGRVIDAAARHFDDVIIDTPPMLGDAVLTAFHRSDELFVLATLDIPSVRNMRVF
                     LDTLDRLNVRTDAVRLFLNKAEDDAGMEVRQIDDLFPRGFDGTLPYSRDVQRSLNAGL
                     PVMAHRPGSAISHRLGDATKRLLDPSQRQAYEAERSEPRSSGRWWRGRSHEAAAS"
     gene            complement(3586..>3795)
                     /locus_tag="VNQ33_04800"
     CDS             complement(3586..>3795)
                     /locus_tag="VNQ33_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97455.1"
                     /translation="GDESTPTTVAPRSTEGQLTLLLALTPAQAEQAIFAASFNHVWFT
                     VLPKDAPPSKTGGVTYEDGYVGVGA"
BASE COUNT          515 a         1294 c         1453 g          533 t
ORIGIN      
        1 ggtctcgacg gcgaggaaca ggccgaccgg catgaggccc aggatccagg ccgagatgcg
       61 gccctcggcg gtgagcaccg cgatctcccg ccgcacctct tcgcggccgc ggatgacctc
      121 ggcgagcgtc gccatcaggt cggtgaggtg gccgccggtc tgctgctgga tccggatcgc
      181 ctgcaccatc cactcgaggt cgcggttgcg catccgcgtc gagaggtgct cgagggagtc
      241 gaccagggac gtgcccagct gcgtctcgct gaccacgcgc ctgagctcgt cggacatcgg
      301 cgactcggcc tcggtcgcca gcagctggat cgagcggagg aacgtgtgcc cggcgctgag
      361 cgacgaggag atgaggacca gggcgtcggg cagctgctgg gcgaagcgct tgttccgccg
      421 gcgggccagg aggtcgagca ccttggaggt gacggccggg gtgacgagga cgacgagcac
      481 cacggcccac cagcgaccgg tccacgccgt ggccgccgct ccgacggcga agcccgagca
      541 cgcggcgatg accaccagct cggaggggcg caccgacagg tcggcgcgtt cgatccgggt
      601 gcgcagcgac gtcgcggacc cggcctgctt gatggcccgt tcggccaggc cgagcgtgcc
      661 cgacaccacg gtgccgtact cggcggcgac ccgggcgacg tcgacgtcct gctcgccgta
      721 ggggagccgc aggaggtcgg cgagctggac ctcgcgggag cggacccggg ccatccagcc
      781 ggcccacagg agggcgagcc cgctgatccc caggcccagg agcagcaggg tggtggtcac
      841 cgctcggcgc cgatcgtccg accggccagc gtgctcgcgt cgggaacctc gatcgggcgg
      901 cgaggcgccg gccccgccgc cggcgcccgg aagacgctgg ccgggagcgg gaaccccttg
      961 gtggcgaggc ggtcggcgaa ggcgggccgg aggccggtgg cgaggagctg gccgacgtcg
     1021 tcggagccgg agacgtcgaa gcggaacacc tcttgcatca cgatggtgtc gccctcgagg
     1081 ccggtgatct ccgagagcga ggtcaccagg cggcgcccgt tgggcagccg ctcgcactgc
     1141 acgacgatgt cgacggcagc ggtgatctgc tcccgcacga cccgggtggg caggtcgaac
     1201 cccgccatca ggaccatcat ctcgagccgg ctcatcgcat cgcgcggcga gttggcgtgg
     1261 accgtggtca tggagccctc gtggcccgtg ttcatcgcct ggagcatgtc gagcgcctca
     1321 gccgaccggc actcgccgat gatgatgcgg tcgggccgca tccgcagggc gttgcggacg
     1381 aggtcccgga tcaccacctc gccctggcct tcggcgttgg cggggcgcgc ctccaagctc
     1441 accacgtggc gctgctgcag ctgcagctcc gcggagtcct cgatcgtgat gatccgctcc
     1501 gatgccggga cgaacgacga catcacgttg aggttcgtcg tcttgccggt gccggtccca
     1561 ccggacacga ggatgttcat gcggccgcgg acgcaggcgt cgagcacggt cacgaggtcg
     1621 gacgtgtagg tcccggtcgc gatgaggtcc atcgctgtca gcgggtccgc ggcgaacttc
     1681 cggatcgtca agaccgggcc gttgatggcg agcggcggga tcacggcgtt gacgcgggag
     1741 ccgtcggaca ggcgggcgtc gaccatgggc gagctctcgt cgacccgtcg gcccacggcc
     1801 aacacgatgc gctcgatcac cttgcggtac tggccttcgt cggcgaacga cacgccggcc
     1861 tcctcgatgc ggccgctgcg ctcgacccag acctcgtcgt agccgttgca catgatctcg
     1921 gtgaccgact cgtcctggag cagcgggtcg agcggcccgt agccgagggt gtcggcgatc
     1981 atctcgttca cgaacgcccg ccgctccatc ggcgagacgg tcacgtcctc ccgctggagg
     2041 atgaggtcga gggcggaccg gaccgcggcg ttgaggtcgt cggggccgag atccgacgcg
     2101 atgggcgcga cctcctcgag caccatcgcc cgcacggcgc gcttggtggc cgtccactgg
     2161 tcgtcgggcc ccggcgtcgg ccgacgtcgc ggtgcgggct cgccggggac gtcgtcgggg
     2221 accgcggcga ggcggtcggc caggctcatg acgccgcggc ctcgtggctg cggccccgcc
     2281 accagcgacc gctggaccgg ggctcggacc gttcggcctc gtaggcctgg cgctggctcg
     2341 ggtccagcag gcgcttggtg gcgtcgccca ggcggtggct gatcgccgag ccgggccggt
     2401 gcgccatcac cggcagcccg gcgttgaggg agcgctgcac gtcgcgggag tagggcagcg
     2461 tcccgtcgaa gccgcgcggg aacaggtcgt cgatctggcg gacctccatg cccgcgtcgt
     2521 cctcggcctt gttgaggaag agccggaccg cgtcggtgcg cacgttcagc cggtccagcg
     2581 tgtcgaggaa gacccgcatg ttccgcacgc tcgggatgtc gagggtggcc agcacgaaca
     2641 gctcgtcgga ccggtggaac gccgtgagca cggcgtcgcc cagcatcggc ggcgtgtcga
     2701 tgatgacgtc gtcgaagtgg cgggcggcgg catcgatcac ccgtccgatg tcctcaggct
     2761 ggatcatcgc cgcctcggtg ggatcccgtg gcgcggccag cacccacacg ccggtctcgt
     2821 cctgctcgca gaactcctcg atgtgggcgt cgagctccga ttgcggctcg tcgccgtgct
     2881 ggatgaggtc ggtgatggtg tagcgggggc ggagctgcag ggcggcggtc acctcgccga
     2941 actggaggtc gaggtcgagg atgcagaccc ggcgcccgcc cttgtgggtc aggaaccagg
     3001 ccaggttcgt ggcgaggaac gtcttgccgc agccccccga cgccgacgcg acggtgatga
     3061 cccgctgggg ccgctccggc ggcggggcgg ggaggggctc ggcggcgctg ggagcggggt
     3121 cgacgatccg cagcgcgagc gatgccgctc ggttgatggt ggcggcgagg cgctcctcgt
     3181 cgatggtgga ctggaccagg tcgaccgcac cggcccggac gaccgtggcg gcgtcgtggt
     3241 ggtcgggtcg gtcgacggcg aggacggtca cgagccgggg gagctcgtcg cggatgatgc
     3301 gcagccgctc gatcccggag cggttgtcca ggcccggccc ggccaccagc acgtcgacgt
     3361 gctgctcggt gaggagccgg cccagctcgc cgaggtcggc gctgcggagc accacgggcc
     3421 gcggcgccaa ggtggcggcc acggcgtcca ccttcgacca cagcgcgccg cgctggtcgt
     3481 ggaccaagac caccggggcg gtctggacca ccgccgtctg caccgcctcg gcgggcggcg
     3541 gctcggacac ctcggcggtc ggaggggcgc tcccgttgac cgagctcatg cgcccacccc
     3601 gacgtagccg tcttcgtagg tgacgccgcc ggtcttgctc ggcggtgcat ccttcggcag
     3661 caccgtgaac cacacgtggt tgaagctcgc cgcgaagatc gcctgctccg cctgcgccgg
     3721 ggtgagcgcc agcagcaggg tgagctggcc ctcggtgctg cgcggggcga cggtggtggg
     3781 cgtcgactcg tcgcc
//
LOCUS       DATMFS010000260         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_32906, whole genome shotgun sequence.
ACCESSION   DATMFS010000260 DATMFS010000000
VERSION     DATMFS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32906"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VNQ33_04805"
     CDS             <1..795
                     /locus_tag="VNQ33_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97456.1"
                     /translation="VLSLVAFFALWWGLSRSGVWDEQFIPRPSAVWDRFIDSIHTRTT
                     TKTTSAGLELVIEKRGLQGSLLWEHWWATVRRILWGLLWAVVVGVPLGLLLTTVNWFR
                     VVFEPWIDFLRSLPPLAYFSLLIIWFGIGDTSKIWLLFLAGVAPITLSIVSGARSIPR
                     DRLLAARALGASRFQTIRYLVLPSVLPDFFNGFRLAVGFAWTTIVAAETTAGLPGIGG
                     LAWASKKTGRSDIVVLCIIVIGITAVLFDFGIKTLERRLLPWRGRG"
     gene            879..1955
                     /locus_tag="VNQ33_04810"
     CDS             879..1955
                     /locus_tag="VNQ33_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWJ97457.1"
                     /translation="MNHQRHRRPALVAVVLAAAISLVAVGCGSSDSGSSKDDPTTTAA
                     AAKDTGKKVDAPKKLRIAYQAIPNGDLIVKQQKLLEKALPGTEIDWQLFDSGGSVNEA
                     VAAGDIDLGLAGSSPVSRGISNGIDYRVVWIHDVIGDAEALVVSDDIGGIEDLAGKTI
                     ATPFASTSHFSLLAALNDAGVDPSTVKIIDSEPDEIYAAWSQGDIDGAYVWNPNLAKI
                     VDDGGKVLITSADLAEKGQTTYDLGVASNAFAEKYPDVLDVWAKAQDQAVSLIQEDPD
                     SAADIIAAELDITPEEAASQIGDLIFVRAADQAGPDYLGGQLAENLYATAQFNKEQGE
                     IDKVLDQDAYQDAVDAAPATSVGS"
     gene            2006..2746
                     /locus_tag="VNQ33_04815"
     CDS             2006..2746
                     /locus_tag="VNQ33_04815"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97458.1"
                     /translation="MAHEFASDRGTVRALDLVDTTIEAGEFVTLAGPSGCGKTTLLQI
                     LAGFLAPSEGTVEVGGHPVTGPDPERGVVFQQATLFPWLSVRGNVEFGPKMAGASKAE
                     RRATADRLLDLVGLSEFGGHHTYELSGGMQQRAQIARVLATDPALLLMDEPFGALDAL
                     TRLRLQDQLRRLWLETGKTVLFITHSVDEAAFLGSRVLVMSPRPGRIVFDEATPYGRG
                     ADLPENPRSTPEFIAFRDRVAGVIHAPV"
BASE COUNT          449 a         1153 c          949 g          450 t
ORIGIN      
        1 gtcctctcgc tcgtcgcgtt cttcgcgttg tggtggggcc tctcgcgctc cggcgtctgg
       61 gacgagcagt tcatccccag gcccagcgcg gtgtgggacc gcttcatcga ctccatccac
      121 acccggacca cgaccaagac gacctcggcc ggcctcgagc tcgtgatcga gaagcggggc
      181 ctccagggtt cgctgctctg ggagcactgg tgggccacgg tgcggcgcat cctgtggggc
      241 ctgctgtggg ccgtggtcgt cggcgtcccg ctcggcctgc tgctcaccac cgtcaactgg
      301 ttccgcgtcg tgttcgagcc gtggatcgac ttcctccgca gcctcccgcc gctcgcctac
      361 ttcagcctcc tgatcatctg gttcggcatc ggcgacacgt cgaagatctg gctgctgttc
      421 ctcgccggcg tggcgcccat caccctctcg atcgtctccg gtgcccgcag catcccgcgg
      481 gaccggctgc tcgccgcccg ggcgctcggt gcgtcgcggt tccagacgat ccggtacctc
      541 gtgctgccct cggtcctgcc ggacttcttc aacgggttcc ggctggcggt cggcttcgcc
      601 tggaccacga tcgtcgccgc cgagaccacc gccgggctcc ccggcatcgg cggcctggcg
      661 tgggcatcga agaagaccgg gcgctccgac atcgtcgtgc tctgcatcat cgtcatcggc
      721 atcaccgccg tgctcttcga cttcggcatc aagacgctcg agcgccggct gctcccgtgg
      781 cgaggccgcg gctgagcgcc cccgatcccg gccgcagatc gcggtcggtc caccgacgcc
      841 caccggcgtc gccgatccgt tccctgggag ggaatctgat gaaccaccaa cgacaccgac
      901 gaccagcgct ggtcgccgtc gtcctggccg ccgcgatctc cttggtggcg gtcggctgcg
      961 gctccagcga cagcggctcg tccaaggacg atccgaccac cacggctgcc gccgccaaag
     1021 acaccggcaa gaaggtcgat gcgcccaaga agctccgcat cgcctaccag gcgatcccga
     1081 acggcgacct catcgtcaag cagcagaagc tgctcgagaa ggccctgccc ggcaccgaga
     1141 tcgactggca gctgttcgac tcgggcggct cggtcaacga ggccgtcgcc gccggcgaca
     1201 tcgacctcgg cctggccggc tcgagccccg tgtcccgcgg catctcgaac ggcatcgact
     1261 accgcgtggt ctggatccac gacgtcatcg gcgacgccga agcgctcgtc gtctccgacg
     1321 acatcggcgg catcgaggac ctcgcgggca agaccatcgc cacgccgttc gcgtcgacct
     1381 cgcacttcag cctgctcgcc gcgctgaacg acgccggcgt cgacccgtcc acggtgaaga
     1441 tcatcgactc ggagcccgac gagatctacg ccgcctggag ccagggcgac atcgacggcg
     1501 cctacgtgtg gaacccgaac ctggcgaaga tcgtcgacga cggcggcaag gtgctgatca
     1561 cctcggccga cctcgccgag aagggccaga ccacctacga cctcggcgtc gcctccaacg
     1621 cgttcgccga gaagtacccc gacgtgctgg acgtgtgggc caaggcccag gaccaggccg
     1681 tctccctgat ccaggaggac cccgactccg ccgcggacat catcgccgcc gagctcgaca
     1741 tcacgccgga ggaggccgcc agccagatcg gcgacctcat cttcgtgcgg gccgccgacc
     1801 aggccggacc ggactacctc gggggccagt tggccgagaa cctctacgcc accgcccagt
     1861 tcaacaagga gcagggcgag atcgacaagg tcctcgacca agacgcctac caggacgcgg
     1921 tcgacgccgc gcctgccacc tccgtcggga gctgaagatg accgtcgcag ggtcacccgg
     1981 gcccgcccgg gtgaccctgt cacgggtggc gcacgagttc gcgtcggacc gtgggaccgt
     2041 gcgcgccctc gacctggtcg acacgaccat cgaagccggc gagttcgtca cgctcgccgg
     2101 gccgtcgggg tgcggcaaga ccaccctcct gcagatcctc gccggcttcc tcgccccctc
     2161 cgaagggacg gtcgaagtcg gcggccaccc cgtcaccgga cccgaccccg agcggggcgt
     2221 ggtgttccag caggcgaccc tgttcccctg gctgagcgtc cgcggcaacg tcgagttcgg
     2281 cccgaagatg gcgggcgctt cgaaggccga gcgccgggcg acagccgacc gcctcttgga
     2341 cctcgtgggg ctcagcgagt tcggcggcca ccacacctac gagctgtccg gcggcatgca
     2401 gcagcgggcc cagatcgccc gcgtgctcgc caccgacccc gcgctgctct tgatggacga
     2461 gccgttcggc gccctcgacg cgctcacccg gctgcggctc caagaccagc tgcgccgcct
     2521 ctggctggag accggcaaga ccgtcttgtt catcacccac agcgtcgacg aggccgcctt
     2581 cctcggatcg agggtgctcg tgatgagccc ccgccccggc cgcatcgtct tcgacgaggc
     2641 cacgccgtac ggccgcggtg ccgatctccc ggagaacccg cgcagcacgc ccgagttcat
     2701 cgccttccgc gaccgcgtgg ccggcgtcat ccacgcaccc gtgtagcgcg gctccgaccg
     2761 ccgcgcgtgt gacggctgac cgcacgcggc ccttgtgatc ggcggtcgca gaggataaaa
     2821 cccgactaat cccatcgtga atagagagtt atctgtgcac cgcctccgaa cctcccgccc
     2881 gatcctggcc ctcgtcctcg tgggcgcctt cgccctcggc ctgctcagcg cctgcagcgc
     2941 gtccgacgcc tccacccggg cgagcgccca agacgacgag ccgacgacca ccgcccccca
     3001 g
//
LOCUS       DATMFS010000261         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_170637, whole genome shotgun sequence.
ACCESSION   DATMFS010000261 DATMFS010000000
VERSION     DATMFS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170637"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2484
                     /locus_tag="VNQ33_04820"
     CDS             <1..2484
                     /locus_tag="VNQ33_04820"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HWJ97459.1"
                     /translation="LIADLDPLAWKEPVMHSELDPATYGLTIWDLEREFLTGGVAGTT
                     RMKLGDLLHVLRDAYCRTIGIEYMHISEPEQKEWIQAHVEGVNDRIDPDEQRHILGRL
                     NAAEAIERFLATKYVGQKRFGIEGAESAIPILDAVLDAAATDGLDGAVLGMAHRGRLN
                     VLTNIVGKSYDQLFKEFEGYIDPDSTQGSGDVKYHLGQTGTFTGRRGHTIELELAANP
                     SHLETVDPVVVGMVRAKQDAINDPEAFSVLPVLLHGDAAFAGQGVVAETLNCSTIKGY
                     RVGGTVHLIINNQLGFTTSPESARSSEYSTDVAKMIEAPIFHVNGDDPEACVRVARLA
                     YEFRQAFHKDVVIDMVCYRRHGHNEGDDPSYTQPLMYKRIDARRSVRKLYTEALVKRG
                     DISLEEAEQALDDFSARLNTVLAETRDAKPESEVIARPVPPAIGVLPHVDTGVELAEL
                     QRINAGLVTVPEGFEIHPKLVKQFDTRSKMVEGGEVDWGTAEAMAFGSLLLEGTDIRV
                     AGQDSRRGTFSHRHSVLSDYSTGAEYAPLAHLSPEQGKFFIYDSLLSEYAAMGFEYGY
                     SVVAKDALVCWEAQFGDFVNGAMIVIDQYLVAAEDKWGQTSGLVLLLPHGLEGQGPEH
                     SSGRVERFLTLCAEDNIQVANATTAAQYFHLLRRQMHREVRKPLVVMTPKSLLRAKVA
                     RSPLSALTAGSFQELLDDPGVTDANAVQRLVFCSGKVAYDAIARRDEKSHPAAIVRIE
                     QLYPFPYQQVTELLTRYPNVSEVVYLQEEPDNMGPRSFVNERLWPLVGEGVKYRQVSR
                     TGSGSPATGSHAIHLQEQSQILDQTFDGL"
BASE COUNT          427 a          979 c          797 g          370 t
ORIGIN      
        1 ctcatcgcgg acctcgatcc gctggcgtgg aaggagcccg tgatgcacag cgagctcgac
       61 cccgccacct acggcctcac catctgggac ctcgagcgcg agttcctcac cggcggtgtc
      121 gccggcacca cccgcatgaa gctgggcgat ctcctccacg tgctgcgcga cgcctactgc
      181 cgcaccatcg gcatcgagta catgcacatc tccgagccgg agcagaagga gtggatccag
      241 gcccacgtcg agggggtcaa cgaccgcatc gatcccgacg agcagcgcca catcctcggc
      301 cgcctgaacg cggcggaggc catcgagcgc ttcctcgcca ccaagtacgt cggccagaag
      361 cggttcggga tcgagggcgc cgagtccgcc atcccgatcc tcgacgccgt gctcgacgcg
      421 gccgccaccg acgggctgga cggtgccgtg ctgggcatgg cccaccgcgg ccgcctcaac
      481 gtgctcacca acatcgtcgg caagagctac gaccagctct tcaaggagtt cgagggctac
      541 atcgaccccg actccaccca gggttcgggc gatgtcaagt accacctcgg ccagaccggc
      601 accttcaccg gccgccgcgg gcacaccatc gagctggagc tggccgccaa cccgagccac
      661 ctcgagacgg tcgaccccgt cgtggtcggc atggtgcgag ccaaacagga cgccatcaac
      721 gaccccgagg cgttctcggt gctgccggtg ctgctgcacg gcgacgccgc cttcgccggc
      781 cagggcgtgg tggccgagac gctcaactgc tccaccatca agggctaccg ggtgggcggc
      841 accgtccacc tgatcatcaa caaccagctc ggcttcacca cctcgcctga gtctgctcgc
      901 tcgtcggagt actccaccga cgtcgccaag atgatcgagg cgccgatctt ccacgtgaac
      961 ggcgacgacc ccgaggcctg cgtccgggtc gcccggctcg cctacgagtt ccgccaggcg
     1021 ttccacaaag acgtcgtcat cgacatggtc tgctaccgcc gccacggcca caacgagggc
     1081 gacgacccga gctacacgca gccgctcatg tacaagcgga tcgacgcccg ccgctcggtc
     1141 cgcaagctct acaccgaggc gctcgtcaag cgcggcgaca tctccctgga ggaggccgag
     1201 caggcgctcg acgacttcag cgcccggctc aacacggtgc tcgcggagac gcgcgacgcc
     1261 aagcccgagt ccgaggtcat cgcccggccg gtgccgcccg ccatcggcgt gctgccccac
     1321 gtggacaccg gcgtcgagct cgccgagctc cagcggatca acgccggact ggtcacggtg
     1381 cccgagggct tcgagatcca cccgaagctg gtgaagcagt tcgacacccg ctccaagatg
     1441 gtcgaaggcg gcgaggtcga ctgggggacc gccgaggcca tggcgttcgg ctcgctgctg
     1501 ctcgagggca ccgacatccg cgtcgccggc caggactccc ggcgcggcac cttctcgcac
     1561 cgccactcgg tcctgtcgga ctactccacc ggcgccgagt acgccccgct ggctcacctg
     1621 tcgccggaac agggcaagtt cttcatctac gactcgctgc tcagcgagta cgccgccatg
     1681 ggcttcgagt acggctactc cgtcgtggcc aaagacgccc tcgtctgctg ggaagcgcag
     1741 ttcggcgact tcgtcaacgg cgccatgatc gtcatcgacc agtacctcgt ggccgccgag
     1801 gacaagtggg gccagacctc cgggctcgtc ctgctcctcc cgcacggcct cgagggccag
     1861 gggccggagc actcctcggg tcgcgtggag cggttcctca ccctgtgcgc cgaggacaac
     1921 atccaggtcg ccaacgccac caccgccgcc cagtacttcc acctgctgcg ccgccagatg
     1981 caccgcgagg tgcgcaagcc gctggtggtc atgacgccca agtcgctgct ccgagccaag
     2041 gtcgcccgct cgccgctgtc ggcgctgacc gcggggtcgt tccaggagct gctcgacgat
     2101 cccggcgtca ccgacgccaa cgccgtgcag cgcctggtgt tctgctcggg caaggtcgcc
     2161 tacgacgcca tcgcccgccg cgacgagaag agccatccgg ccgccatcgt ccggatcgag
     2221 cagctctacc cgttcccgta ccagcaggtc accgagctgc tgacccgcta cccgaacgtc
     2281 agcgaggtcg tctacctcca ggaggagccc gacaacatgg gcccccgctc gttcgtcaac
     2341 gagcgcctct ggccgctggt gggcgagggc gtcaagtacc gccaggtctc gcgcaccggc
     2401 tccgggagcc cggccaccgg ctcccacgcc atccacctcc aagagcagtc ccagatcctg
     2461 gatcagacct tcgacggcct ctaggccgcc ggaggctcgg gccggccgcg ttcggacccg
     2521 tcgatacacc acgtgcgccg catcgtcggc tgctacgtgg cggcgttcgc cct
//
LOCUS       DATMFS010000262         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_264059, whole genome shotgun sequence.
ACCESSION   DATMFS010000262 DATMFS010000000
VERSION     DATMFS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264059"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..841)
                     /locus_tag="VNQ33_04825"
     CDS             complement(101..841)
                     /locus_tag="VNQ33_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97460.1"
                     /translation="MLWLQNARGSSAVEAGMLMLPSTIGIFAFIPLGGRMSQRRGSRT
                     PVLVGLVVMAAGLVVVGQLSVDSTLVVLGAGLVIVGLGLGLLSTPVSNTAVGEAPPEL
                     AGTAAGVFKMSSMVGGALGVAVLAAIARSATQGDVDVAAKAAGMSGDEIDQAHRALVQ
                     STSFTDAIAKLPADLAQKVTDAAIRAFTDGVAQSLGDHRRPGDRRHGRGRLPLAGPRA
                     RGPERTHRSIRHDRLRVRRLLRSCHVHR"
     gene            complement(846..>2091)
                     /locus_tag="VNQ33_04830"
     CDS             complement(846..>2091)
                     /locus_tag="VNQ33_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97461.1"
                     /translation="LFIGSCVLVALATSGLAVIAGRAIQGAAGSTLLASGLSLLTIAT
                     DGPGRLRAVSYWGSASAIGAAAGPLIGGLLVDTMGWQGLFWIDAAIAVVLVPVTLRSV
                     GESSDPNRSRSIDWIGTVLIALVLAPFILGVSEGSDWGWTSPATLACFVVSIASIVGF
                     AKVEATVSAPLVDLRLLRNRLLIGATLGILIGAATINGLMFVLSLFFQDPSTLDMNPL
                     QAGLATLPATIGMIALTPFVPKLASRFGTRSVIGFGFIAMTVGFALLMAVDSDWGYLM
                     FVLPVVLAAIGMCLSNNPCSSVATSSVDEAQVGAASGISNMARYVGAAVMTAIVAALY
                     SVVSTNQAADGAATAEALASGFRSAAVALTAVSAAGIALVYLVGQHRPAHAGTGVDVA
                     IAAAAPSHTIPRPSAAAPVAAT"
BASE COUNT          338 a          703 c          806 g          244 t
ORIGIN      
        1 cggcggcatc tgggcggcgt cgtgcaccga cttgaacgag accatctcgg ccaggtcggc
       61 cttggcccgg ggcatcagcc cctggatcgt ggtgagcagg tcatcggtgg acatggcagc
      121 tcctcaggag acgtcggaca cggagtcggt cgtgacggat ggatcggtgg gttcgttccg
      181 gcccgcgggc ccgcgggccc gccagaggaa ggcgaccgcg gccgtggcga cgatcgccag
      241 gacgccggtg atcacccagg ctctgggcga cgccgtcggt gaaggcgcgg atggcggcgt
      301 cggtcacctt ctgggcgagg tcggcgggca gcttggcgat ggcatcggtg aacgaggtcg
      361 actggaccag ggcgcggtgc gcctggtcga tctcgtcgcc gctcatgccg gccgccttgg
      421 cggcgacgtc gacgtcgccc tgcgtggcgc tgcgggcgat ggcggcgagc acggccacgc
      481 cgagcgcgcc gccgaccatg ctcgacatct tgaagacgcc ggcagcggtg ccggccagct
      541 caggcggggc ctcgccgacc gcggtgttcg agaccggcgt cgacagcagg cccaggccca
      601 agccgacgat caccaggccc gcacccagca cgacgagggt ggagtccacg ctgagctggc
      661 cgacgaccac gagcccggcg gccatgacca ccaggccgac gagcacgggg gtccggctgc
      721 cccgcctctg cgacatccgg ccgccgagcg ggatgaaggc gaagatgccg atggtcgacg
      781 ggagcatcag catcccggcc tcgacagcgc tggagccccg ggcgttctgc agccagagca
      841 cgaggtcacg tcgccgcgac cggtgcggcg gcgctcgggc gcgggatggt gtgggagggc
      901 gctgccgcgg cgatggccac gtcgactccc gttccggcgt gggccgggcg gtgctggccg
      961 acgaggtaga cgagggcgat gccggccgcc gagacggccg tcagcgcgac cgccgcgctg
     1021 cggaacccgc tcgccagggc ctcggccgtg gccgcgccgt cagccgcctg gttcgtggac
     1081 accaccgagt agagggcggc gacgatggcg gtcatgaccg cggcgccgac gtagcgggcc
     1141 atgttcgaga tgcccgaggc cgcgcccacc tgggcctcgt cgaccgagct ggtggccacc
     1201 gacgagcacg ggttgttcga gaggcacatg ccgatggcgg ccagcacgac cgggagcacg
     1261 aacatcaggt agccccagtc cgagtcgacc gccatcagca gggcgaagcc gaccgtcatg
     1321 gcgatgaacc cgaacccgat caccgagcgg gtgccgaacc gcgaggccag cttcgggacg
     1381 aacggggtca gggcgatcat gccgatggtc gccgggaggg tggccaggcc ggcctggagc
     1441 gggttcatgt cgagcgtcga cggatcctgg aagaacaggc tgagcacgaa catgaggccg
     1501 ttgatcgtgg cggcgccgat gaggatgccg agggtggccc cgatgaggag ccggtttcga
     1561 aggagcctca ggtccaccag gggagcggac acggtggcct cgaccttggc gaaccccacg
     1621 atggaggcga tcgacacgac gaagcacgcc agcgtcgccg gcgacgtcca gccccagtcc
     1681 gaaccctcgc tcacgccgag gatgaacggg gcgaggacga gggcgatcag gaccgtgccg
     1741 atccagtcga tggagcggct ccggttcgga tccgaggact cgcccacgga ccgcagcgtg
     1801 accggcacga gcacgacggc gatggcggcg tcgatccaga acaggccctg ccagcccatc
     1861 gtgtccacca gcagcccgcc gatgagcggt ccggcggcgg ctccgatggc cgacgcggat
     1921 ccccagtagg agacggcgcg cagccgcccg gggccgtcgg tggcgatggt gaggaggctc
     1981 aggccgctgg ccaggagggt cgaccccgcg gcgccctgga tggcccgccc ggcgatgacg
     2041 gccagcccgc tggtggccag ggcgacgagc acgcacgatc cgatgaacag c
//
LOCUS       DATMFS010000263         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_170767, whole genome shotgun sequence.
ACCESSION   DATMFS010000263 DATMFS010000000
VERSION     DATMFS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170767"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VNQ33_04835"
     CDS             <1..454
                     /locus_tag="VNQ33_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97462.1"
                     /translation="TTTAAPTTTAAPTTTTSSTVIPGSSTTLAPTTTAVPATTTPDET
                     PTTVAEGSTTLAPTPSTTLPEQVTVPGSEVEGTTTMPGTDLTGGTASTSTTAGGTGTS
                     GGSDTPGTTTVELARTGSDSGRLAGIGALTLAAGCCFVVASRRRIRSA"
     gene            complement(514..900)
                     /gene="ssrA"
                     /locus_tag="VNQ33_04840"
     tmRNA           complement(514..900)
                     /gene="ssrA"
                     /locus_tag="VNQ33_04840"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1003..1791)
                     /locus_tag="VNQ33_04845"
     CDS             complement(1003..1791)
                     /locus_tag="VNQ33_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97463.1"
                     /translation="MPPSPPRPRSRHRIVEVALVLALTGAIVVLAFTAVDGGPRATAG
                     SKFVTVGQSIAGSDSDHSSGEWDTDPMDDEEPFAATGDLFAALPADEDIEDLLEISDG
                     RVTMQIEEYLEPGPAKAFCSDVAPDAVEGAWRAWTASGGGVEVDARIHELGSVAEARR
                     AIESRQTDAFLACIPDGVESSVRHMQVDGGEITDSSTTTTGDQLEQILLIERGEEGTL
                     FAWRQVDRYVVTIKVFDFDNDEGAALDLTSADDLLALSTEALGG"
     gene            complement(1847..2326)
                     /gene="smpB"
                     /locus_tag="VNQ33_04850"
     CDS             complement(1847..2326)
                     /gene="smpB"
                     /locus_tag="VNQ33_04850"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWJ97464.1"
                     /translation="MAPKRTGDNVVATNRQAHRDYEILDTWEAGIMLKGSEVKSLREA
                     KVQLRDAYARFTGRELFLVGLHIASYSNASAQGGHELERTRKLLLHEHELTEMKARID
                     QEHLTLVPLSLYFKKGRAKLELGIGKGRKHYDKRQVIAQRDSDMDAKRAMAARRRGE"
     gene            2385..2798
                     /locus_tag="VNQ33_04855"
     CDS             2385..2798
                     /locus_tag="VNQ33_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HWJ97465.1"
                     /translation="MLRIARSAHLAMIRHAVAGLPNEACGLLGGPVGGGHVSAFAPTR
                     NADASAKTYSIAVDGWTEADRIFLPLGIDAVGIMHSHTHTDPYPSPTDVDSANPMLMG
                     FHYVIVSLRDREPMLRSWLLDDGRIVETPVAIVEG"
     gene            2976..>3466
                     /locus_tag="VNQ33_04860"
     CDS             2976..>3466
                     /locus_tag="VNQ33_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HWJ97466.1"
                     /translation="MAVYDSVTDLIGNTPIVDVSALSPNPNVRILAKLEGQNPGGSVK
                     DRAAKWMIEAAEASGDLKPGMTVLESSSGNTGIALAMICKMKGYPFKVVLPENVSEER
                     RQALEVWGSEIISSPAGEGSNGAMRRARVLAADNPEWFFPFQYGNDANPRAHYETTGP
                     EIWR"
BASE COUNT          506 a         1197 c         1212 g          551 t
ORIGIN      
        1 gaccaccacg gcggcgccga ccaccacggc ggcgccgacc accacgacgt cgagcacggt
       61 gatccccggc agttcgacca cgctcgcccc gacgacgacc gcggtccccg ccacgacgac
      121 ccccgacgag accccgacga cggtggccga gggctccacg acgctcgccc cgacaccgtc
      181 cacgacgctg cccgagcagg tcacggtgcc aggcagcgag gtcgagggca ccacgaccat
      241 gccggggacc gacctgaccg gcgggaccgc ctcgacgtcc accacggcgg gcgggaccgg
      301 gacatctggc ggttccgaca cgccggggac cacgaccgtc gagctcgctc ggaccggctc
      361 ggactccggg cggctggctg ggatcggcgc cttgaccctc gccgctgggt gctgtttcgt
      421 ggtggcgagc cgacgccgca tccggtcagc ctgaggccga cacgaccgct ggcctgggca
      481 ggtgcccagg ccaggagatc gagacaagtg ggttggtgga gctggggaga atcgaactcc
      541 cgtccgcgag gcggttgacg accgcgctac gaccgttccc gagacgagcc gtgacggctg
      601 gctccctgcc gggtcaggtg ggcttgcgcc ctccgcctcg tctttccgag gcgtcagcgg
      661 tctttctcgc cgtcagtggt ctttcccacc gtcaaccgct acttctgttg ccgggctgga
      721 gcggtcaggc cccgtgcgcc attgctggtc acgatgtctc tcttggctag atcagtgaat
      781 gatcaggcgg cgagagcgaa gtcgccatcg gcgcttctgt tggttccccg tttaacgagt
      841 ctgagaaact cgggtcgcac gttcggcttc caggtcccca cgtcgaaacc ggtcagcccc
      901 ttgtcaagca accgcccacg gtcacgtggt ggatgggcgg ctggaccagc gtaccggccg
      961 gcgcgggaag gaccaccagg ggttcggggg ctcgacctct ggtcagccgc cgagggcctc
     1021 ggtggagagc gccaagaggt cgtccgccga ggtgaggtcg agcgcggcgc cttcgtcgtt
     1081 gtcgaagtcg aacaccttga tcgtcacgac gtaccggtcc acctgccgcc aggcgaacag
     1141 cgtgccctcc tctccccgtt cgatgagcag gatctgctcg agctggtcac ccgtcgtcgt
     1201 cgtggacgag tcggtgatct cgccgccgtc gacctgcatg tgccggacgc tcgactccac
     1261 cccgtcgggg atgcaggcga ggaacgcatc ggtctggcgg ctctcgatcg cccgccgggc
     1321 ttccgccacg gagcccagct catggatccg ggcatccacc tcgacaccgc cgcccgatgc
     1381 ggtccaggct cgccaggctc cctccaccgc gtcgggcgcc acatcgctgc agaaggcctt
     1441 ggccggtcca ggttcgaggt actcctcgat ctgcatggtg acgcgtccgt cggagatctc
     1501 gaggaggtcc tcgatgtcct cgtcggcggg gagggcggcg aagaggtcgc cggtggctgc
     1561 gaacggctcc tcgtcgtcca tcgggtccgt gtcccactcg cccgacgagt gatccgagtc
     1621 ggagccggcg atcgactggc cgaccgtcac gaacttcgac ccggccgtcg ctcggggacc
     1681 gccgtcgacg gccgtgaagg ccaggacgac gatggctccg gtgagggcca ggacgagggc
     1741 gacctcgacg atccggtggc gcgaccgggg ccgtggcggc gagggcggca cgtgcccctt
     1801 cgacgtcacc ggctaccggg cggagccggt ccccgggctg ctcgggctac tccccgcggc
     1861 ggcgggcggc catggcgcgc ttggcgtcca tgtcggagtc gcgctgggcg atcacctggc
     1921 gcttgtcgta gtgcttgcgg cccttgccga tgccgagctc cagcttggcc cggcccttct
     1981 tgaagtagag ggagagcggg acgagcgtga ggtgctcctg gtcgatccgg gccttcatct
     2041 cggtgagctc gtgctcgtgg agcaggagct tgcgggtgcg ctcgagctcg tggccgccct
     2101 gggccgaggc gttggagtac gacgcgatgt gcaggccgac gaggaacagc tctcgtccgg
     2161 tgaagcgggc gtaggcgtcg cgcagctgca ccttggcctc gcgcagcgac ttcacctcgc
     2221 tgcccttcag catgatgccg gcctcccagg tgtcgaggat ctcgtagtcc cgatgggctt
     2281 gccggttggt ggcgacgacg ttgtcgcccg ttcgcttggg tgccatgacg ggggcgaggc
     2341 taccggtcgg ctgggctccg ccacccggcc actacggtga cgccgtgctg cgcatcgccc
     2401 ggtcggccca cctcgccatg atccgccatg cggttgccgg cctgccgaac gaggcgtgcg
     2461 gcctgctggg cggtccggtc ggcggcggcc acgtgtcggc gttcgcgccc acccgcaacg
     2521 ccgatgcgtc ggcgaagacg tactcgatcg cggtcgatgg ctggaccgag gcggatcgga
     2581 tcttcctccc gctcggcatc gacgccgtgg ggatcatgca ctcgcacacc cacaccgacc
     2641 cgtacccgtc gccgacggac gtcgactcgg ccaacccgat gctgatgggg ttccactacg
     2701 tgatcgtgtc gctgcgcgac cgcgagccga tgctgcgctc gtggctcctc gatgacggca
     2761 ggatcgtcga gacgcccgtc gccatcgttg agggctgacc cggctcctcg tccccgccca
     2821 gccgcggctg ccaaggcgga cgtcgtaggc gagcgcgctc gaaagggcgt tcgaaacggc
     2881 gtcgtgaccg acgaagttcg ggtggggttt gggggatcgg cgcgatgggc ctcctagaat
     2941 cctgagcgtt ccagtcacct ttcgaggagc ccaccgtggc ggtctacgac agcgtcaccg
     3001 atctcatcgg caacaccccg atcgtcgatg tcagcgcgct gagcccgaac ccgaacgtcc
     3061 ggatcctcgc caagctcgag ggccagaacc cgggcggatc ggtcaaagac cgcgccgcca
     3121 agtggatgat cgaggcggcc gaggccagcg gtgacctgaa gccgggcatg accgtgctgg
     3181 agtcgtcgtc gggcaacacc ggcatcgccc tggcgatgat ctgcaagatg aagggctacc
     3241 ccttcaaggt cgtcttgccg gagaacgtgt ccgaggagcg gcgccaggcc ctcgaggtgt
     3301 ggggatcgga gatcatctcg tcaccagcgg gcgaagggtc gaacggtgcg atgcgccgcg
     3361 ctcgggtgct cgccgccgac aaccccgagt ggttcttccc gttccagtac ggcaacgacg
     3421 ccaacccgcg tgcgcactac gagaccaccg ggccggagat ctggcg
//
LOCUS       DATMFS010000264         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_33150, whole genome shotgun sequence.
ACCESSION   DATMFS010000264 DATMFS010000000
VERSION     DATMFS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33150"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VNQ33_04865"
     CDS             <1..264
                     /locus_tag="VNQ33_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWJ97467.1"
                     /translation="IAERVAPEIGADGVTYDPSIAKVSLVGAGMKTNPGVAATVFETL
                     AANHINIEMISTSAIRISCVVAGEQCDAAVAALHTAFGLDHPS"
     gene            392..1360
                     /locus_tag="VNQ33_04870"
     CDS             392..1360
                     /locus_tag="VNQ33_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWJ97468.1"
                     /translation="MGIGAGLLLAVGLLGACGETGTEKSSQTSGDLASGAGSGESAAL
                     KAELAKGPAAGTQIEDRKVVYHGSLTIRVANAEKAADEAIGSATDVNGYLGKQDSQLE
                     GDREVSVTLRIPAADFDDVMAKVAGLGKVQARKVDSDDVTDQVVDLEGRIENARASTE
                     RLRALLAKAENIDNIAALEDRLTQREAEIEQISGQLEVIQDDVQFATVRATFTEKDEP
                     AVSDDLPGPLQSLKAGLVAFVTVALGLLAALAFALPFLLVALVVWLGYRRWRRSHPKP
                     ERPVPPPWPGSTPLPAPPDPVPAGAAAPPEPPSGQTPDPTAEPPAT"
     gene            1410..2429
                     /locus_tag="VNQ33_04875"
     CDS             1410..2429
                     /locus_tag="VNQ33_04875"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWJ97469.1"
                     /translation="MRVGIVGATGQVGGVMRGILAERDFPVTELRLFASARSVGRTLD
                     WKGTEIAIEDAAAADFSGLDIVLFSAGGATSKELAPKVAAAGAVVIDNSSAWRMDPDV
                     PLVVAEVNGHAATARPKGIIANPNCTTMAAMPVLGPLHREAGLKTLVVSTYQAVSGGG
                     LAGVAELDEQAKKVVDQAAGLTFDGQAVDFPEPQKFPAPIAFNVVPKAGNIVDDGLEE
                     TDEEQKLRNETRKILDVPELLVSGTCVRVPVFTGHSLSINAAFERPITPEQARQILDY
                     APGVELSEIPTPLQAAGADPTYVGRIRVDSTVEHGLALFLSNDNLRKGAALNAVQIAE
                     LVASE"
     gene            complement(2644..>2842)
                     /locus_tag="VNQ33_04880"
     CDS             complement(2644..>2842)
                     /locus_tag="VNQ33_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97470.1"
                     /translation="LAQAVRKGTVEQQRHELSDVLAWLASLAHQLDLSLEDAAQRYAD
                     GCPRCGSIPCSCPSFPLSAER"
BASE COUNT          436 a         1020 c          991 g          395 t
ORIGIN      
        1 atcgccgagc gggtcgctcc cgagatcggc gccgacggcg tcacctacga cccgtccatc
       61 gccaaggtgt cgctcgtggg cgcagggatg aagaccaacc cgggcgtggc cgccacggtc
      121 ttcgagaccc tggctgcgaa ccacatcaac atcgagatga tctcgacgtc ggccatccgc
      181 atctcgtgcg tggtggccgg tgagcagtgc gacgccgccg tggctgcgct ccacaccgcc
      241 ttcgggctcg atcaccccag ctgagatccc ggccccgcag gggcgacctt gtgacggccg
      301 acacaggtcg tacgctccgt ccatggccaa cacggcggaa ccagggcggg tgatcggtcg
      361 tcggaccggc atgagccgaa cgaagcggtg gatgggtatc ggggccggtc tgctcctggc
      421 ggtgggcctg ctcggtgcgt gtggggagac cgggaccgag aagtcgtccc agacctccgg
      481 agaccttgcg tccggcgcag ggagcgggga gtcggccgcc ctgaaggccg agctggccaa
      541 gggcccagct gccggaaccc agatcgagga ccgcaaggtc gtgtaccacg ggtcgctcac
      601 gatccgggtg gcgaacgccg agaaggcggc cgacgaggcg atcggctccg ccacggacgt
      661 gaacgggtac ctcggcaagc aggattccca gctcgagggc gaccgagagg tctcggtgac
      721 cctccgcatc cccgccgccg acttcgacga cgtgatggcg aaggtggccg gcctcgggaa
      781 ggtccaggcc cgcaaggtcg actccgacga cgtcaccgac caggtggtcg acctcgaagg
      841 ccgcatcgag aacgcccgag ccagcaccga gcggctgcgg gcgctgctcg ccaaggcgga
      901 gaacatcgac aacatcgccg ccctcgagga ccgcctcacc cagcgggagg ccgagatcga
      961 gcagatctcc ggccagctcg aggtgatcca agacgacgtg cagttcgcca cggtgcgggc
     1021 cacgttcacc gagaaggacg agccggcggt gtccgacgac ctgcccgggc cgctgcagtc
     1081 gctgaaggcc ggcctcgtgg ccttcgtgac cgtcgcgctc gggctgctcg ccgccctcgc
     1141 cttcgccctg ccgttcctgc tggtggccct ggtggtctgg ctcgggtacc ggcgttggcg
     1201 ccgcagccac ccgaagcccg agcgcccggt cccgccgccg tggccgggga gcacgcccct
     1261 gccggcgccg cccgatccgg tgcccgctgg agccgctgcc ccgcccgaac caccgtcggg
     1321 tcagacgccc gacccgacgg cggaaccgcc agccacctga gcgaatccgc cttggggagc
     1381 gccggggggt caccggtagc ctgatcgtca tgcgcgtagg catcgtgggt gcaacaggtc
     1441 aggtcggcgg cgtcatgcgg ggcatcctcg ccgagcggga cttcccggtc accgagctgc
     1501 ggctgttcgc gtcggcccgg tcggtgggcc gcaccctgga ctggaagggc acggagatcg
     1561 ccatcgagga cgcggccgcg gccgacttct cgggcctcga catcgtcttg ttcagcgccg
     1621 gtggcgccac ctcgaaggag ctcgctccca aggtggcggc ggccggcgcc gtggtcatcg
     1681 acaactcgtc ggcgtggcgc atggaccccg acgtccccct ggtggtggcg gaggtcaacg
     1741 gccacgccgc caccgcccga ccgaagggca tcatcgccaa cccgaactgc accaccatgg
     1801 ccgccatgcc ggtgctgggt ccgctgcacc gcgaggccgg gttgaagacg ctggtcgtct
     1861 ccacctacca ggccgtatcg ggaggtgggc tcgccggggt ggccgagctc gacgagcagg
     1921 cgaagaaggt cgtcgaccaa gccgccgggc tcaccttcga cggtcaggcg gtcgacttcc
     1981 cggagccgca gaagttcccg gcccccatcg ccttcaacgt ggtgcccaag gccggcaaca
     2041 tcgtcgatga cggcctcgag gagaccgacg aggagcagaa gctccgcaac gagacccgca
     2101 agatcctcga cgtcccggag ctgctcgtgt caggcacctg cgtgcgggtc ccggtcttca
     2161 ccggccactc gctgtcgatc aacgccgcct tcgagcgccc catcacgccg gaacaggccc
     2221 gccagatcct cgactacgca cccggcgtgg agctgtcgga gatccccacg ccgttgcagg
     2281 ctgccggtgc cgaccccacc tacgtgggcc gcatccgggt cgacagcacg gtcgagcacg
     2341 gcctggcgct gttcctctcg aacgacaacc tccgcaaggg cgcggccctc aacgccgtcc
     2401 agatcgcgga gctcgtcgcc tcggaatgag gctgcacctc atccgaagcg acgatgtggc
     2461 tcgatcgttg gctacggctg caaccgtggt cgctgcttgc ggttctcccc gccgacttcg
     2521 tcgttgagct agccatgccg tcttgatccg ctctgggtgt ctccggccct ggggcctccg
     2581 acgcccgggc cgctcgggcc ctcgccttcg gcgacaagct ggcgctggcg gtcacaggcc
     2641 cgctcagcgc tcagcgctca gagggaacga ggggcaggaa caggggatcg acccgcagcg
     2701 agggcagccg tccgcgtagc gctgggcggc gtcctcgagc gacaggtcca gctggtgggc
     2761 cagcgaggcc agccaggcca gtacgtcgga gagctcgtgg cgctgctgct ccaccgtgcc
     2821 cttgcgcacg gcctgggcga gc
//
LOCUS       DATMFS010000265         3527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_79455, whole genome shotgun sequence.
ACCESSION   DATMFS010000265 DATMFS010000000
VERSION     DATMFS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79455"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNQ33_04885"
     CDS             <1..192
                     /locus_tag="VNQ33_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWJ97471.1"
                     /translation="VHEASTGAPVLRQAVAYLDCEVRQAVEVGNHTLFLGEVVDTAFL
                     KDEETPVLRMEDTRMNYGG"
     gene            267..1772
                     /locus_tag="VNQ33_04890"
     CDS             267..1772
                     /locus_tag="VNQ33_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWJ97472.1"
                     /translation="MASAVSDPVVAAAIDEIDANLDVLLAVEWLPSDCDEAMVRVREY
                     EALSRRLHAVTLRLLDAIGRSGVSRADGHASAKVMVRHGANLSNAQARRRDQVVRMQR
                     EMPAVAAGHAAGRIGVPQVERIARTWANARVRDQLVQVDAQMARAAAMLPYREFDEAL
                     TNWERLADEDGAGDRAERNDANRDFRMPHNLDGSGRFEGGGGSLDTQIIEDIWQRQVR
                     REWEADWAEARCRLGDGATTADLVRTDGQRRFDAMKTCMVRGDLVEYGVEARTCTDLV
                     QDVITFERYLAKIFGTDPGPDPRLATWWDDLLDTLAEPVPSETEPRPESGLESEVAPE
                     SEVDPVSEADPGAAPEPEADPGTTPPAGRPPTSDLVGYRCGTIDGRPLDPAEVVVATL
                     IGQVRRVVTGADSVVLDMGRRARCFTGARQLAVRLSNSSCVWTGCDVPSSECQCDHLD
                     AFNGPAEGRTHPGNGAPQCGPHNRFKEGGFTVVRDRRGRWHVFRPDGTEIT"
     gene            1852..3093
                     /locus_tag="VNQ33_04895"
     CDS             1852..3093
                     /locus_tag="VNQ33_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ97473.1"
                     /translation="MSDTPRIPEHGSDAKELLALIDQRHAEDIDWRGGKAFSLVYNVD
                     DHEHEELIEQVALRYIHDNALNPFKYPSVLQMELDVISMACQLLGTEPDAGSLSSGGT
                     ESIFLAVQVARDHARKERGIAEPQLLTPSTAHPAFAKAAKYLDLEHILIPVGDDGRAD
                     TAATEAAITERTGLIVGSAPCYPYGVIDDIPALAGLALERDILFHTDACLGGWILPWW
                     ERLGEAVAPWDFRVPGVTSISADIHKYGYTFKGASTVLYKSRDLLSHQFFWYDDWPGG
                     LYASGTAAGTRSAAPIAGAWAAINHLGEDGYLRLTEIVRDTTRKMQAGIGAIDGLRIT
                     HAPDLSLFEIGSDTLDIGAVGDVMDDRGWNLDRQQGGLHLMLSPYHARIADQFLADLA
                     DGAATTEASRGKAASYGGLAT"
     gene            3107..>3527
                     /locus_tag="VNQ33_04900"
     CDS             3107..>3527
                     /locus_tag="VNQ33_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWJ97474.1"
                     /translation="MTTVVGAVLVGGASSRMGTDKALLPLEGAAMAHRVVAALVAGGA
                     TAIEVIGGDPAIARALDLSDLIDGWGARDDLWKGIGPIGGLATAVIEARPPATSDPGP
                     LPANVEAATAAAARDDVIVVVAACDQPDLTGAVVDSLV"
BASE COUNT          540 a         1249 c         1258 g          480 t
ORIGIN      
        1 gtgcacgagg cctccaccgg cgctccggtc ctgaggcagg ccgtcgccta cctcgactgc
       61 gaggtccgcc aggccgtcga ggtcggcaac cacacgctct ttctcggcga ggtggtcgac
      121 accgccttcc tcaaggacga ggagaccccg gtcctgcgca tggaggacac gaggatgaac
      181 tacgggggct gaccggcctc cgccgatgtc cgatttcgtg ctgtttcagg ggtttcgctg
      241 tcagactgcc ttcgtacgat ctgggtatgg cttcggcagt ctcggatccg gtggtggcgg
      301 cggcgatcga tgagatcgac gcgaacctcg acgttctcct ggccgtcgag tggttgccgt
      361 cggactgcga cgaggcgatg gtgcgggtcc gggagtacga ggcgctgagt cggcggctcc
      421 acgccgtgac gttgagactc ctcgatgcga tcggccggtc cggggtctcc agggcagatg
      481 gccacgcgtc ggcgaaggtg atggtccggc acggcgcgaa cctgtcgaac gcgcaggcgc
      541 ggcgccggga ccaggtggtg cggatgcaac gggagatgcc ggccgtggcg gcagggcatg
      601 cggccgggcg gatcggcgtg ccacaggtcg aacggatcgc ccgcacctgg gcgaacgcac
      661 gggtccgcga ccaactggtg caggtggacg cccagatggc tcgagccgcg gccatgctcc
      721 cataccgcga gttcgacgag gcgctgacga actgggagcg cctcgcggat gaagacggcg
      781 cgggtgatcg ggcggagcgc aacgatgcga accgggattt ccggatgccc cacaacctcg
      841 acggctccgg gcgcttcgag ggtggcggcg gatcactcga cacccagatc atcgaggaca
      901 tctggcagcg ccaggtgcgg cgcgagtggg aagcggactg ggccgaggcg cgctgccggc
      961 tcggcgacgg ggccacgacg gcggacctgg tccgcaccga cggtcagcgc cgcttcgacg
     1021 ccatgaagac ctgcatggtt cgcggggacc tcgtggagta cggcgtcgag gctcgcacgt
     1081 gcactgatct ggtgcaggac gtgatcacgt tcgagcgcta cctcgccaag atcttcggca
     1141 ccgaccccgg acccgacccc cgtctcgcca cctggtggga cgacctcctc gacaccctcg
     1201 ctgaacccgt cccttccgag accgagccga ggcccgaatc cggcctcgag tcggaggtgg
     1261 ccccagagtc ggaggtggac ccagtgtcgg aggcggaccc cggggcggcg ccggagccgg
     1321 aagccgatcc gggcacgaca cctccagccg gcaggccccc gaccagcgac ctcgtcgggt
     1381 accggtgcgg gacgatcgac gggcggccgc tcgacccggc tgaggtcgtg gtcgccacgc
     1441 tgatcgggca ggtgcggcgg gtcgtgaccg gcgcggactc cgtggtgctg gacatgggcc
     1501 gcagggcgcg gtgcttcacc ggtgcccggc agctcgcggt gcgtctctcg aactcgtcat
     1561 gcgtgtggac cggctgcgac gtcccctcca gcgagtgcca gtgcgaccac ctcgacgcct
     1621 tcaacggacc agcggaaggc agaacccacc ccggcaacgg ggcaccccaa tgcgggccgc
     1681 acaaccggtt caaggaaggg ggcttcaccg tcgtccgaga ccgccgcggc cggtggcacg
     1741 tcttccgacc ggacggcacc gagatcacct gagccgccct ccccagggca ggcgctccgg
     1801 cgcggaccac ggccgggcgc gcccgctgac ctgctggcct aggttctggc catgagcgat
     1861 acgccacgga tcccggagca cggcagcgat gcgaaggagc tgctggcgct gatcgaccag
     1921 cggcacgcag aggacatcga ctggcggggc gggaaggcgt tcagcctcgt ctacaacgtc
     1981 gacgaccacg agcacgagga gctcatcgag caggtcgccc tccggtacat ccacgacaac
     2041 gccctgaacc cgttcaagta cccgagcgtg ctccagatgg agctcgacgt catctccatg
     2101 gcgtgccagc tgctcggcac cgagcccgac gccggctcgc tcagctccgg cggcacggag
     2161 tcgatcttcc tggccgtcca ggtggctcgc gaccacgctc gcaaagagcg ggggatcgcc
     2221 gaaccgcagt tgctgacgcc gtcgacggcg caccccgcct tcgccaaggc ggccaagtac
     2281 ctcgacctcg agcacatcct catcccggtg ggcgacgacg gccgggccga caccgccgcc
     2341 accgaagcag cgatcaccga gcgcacggga ctcatcgtcg gctcggcgcc ctgctacccg
     2401 tacggggtca tcgacgacat cccggcgctg gccgggctgg cgctcgagcg cgacatcctc
     2461 ttccacaccg acgcctgcct cggcggctgg atcctgccct ggtgggagcg cctcggcgag
     2521 gcggtggcgc cgtgggactt ccgggtgccg ggcgtgacgt cgatctcggc cgacatccac
     2581 aagtacggct acacgttcaa gggcgcgtcg accgtgctct acaagagccg ggacctcctg
     2641 agccaccagt tcttctggta cgacgactgg cccggcgggc tctacgcgtc gggcacggcg
     2701 gccggcaccc ggagcgcggc gcccatcgcc ggtgcgtggg cggccatcaa ccacctgggc
     2761 gaggacggct acctgcggct cacggagatc gtgcgggaca cgacccggaa gatgcaggcc
     2821 gggatcgggg ccatcgacgg gctgcggatc acccacgcgc cggacctgtc gctgttcgag
     2881 atcggctcgg acacgctcga catcggggcc gtgggcgacg tcatggacga ccgcggctgg
     2941 aacctcgacc gccagcaggg cgggctgcac ctgatgctgt cgccgtacca cgcccgcatc
     3001 gccgaccagt tcctcgccga cttggccgac ggcgccgcca ccaccgaggc gagccgcggc
     3061 aaggcggcct cctacggcgg cctcgccacc tgagcgaggc gacccggtga ccaccgtcgt
     3121 cggcgcggtg ctcgtgggcg gggccagctc ccgcatgggg accgacaagg ccctgctccc
     3181 gctcgaaggg gcggcgatgg cccaccgcgt cgtcgccgcg ctcgtcgccg gcggcgcgac
     3241 ggcgatcgag gtcatcggcg gcgatccggc catcgcacgc gccctggacc tctcggacct
     3301 gatcgacggg tggggagccc gcgacgacct ctggaagggc atcggcccca tcggtggcct
     3361 ggctaccgcg gtgatcgagg ctcgtccacc ggcgacctcg gatcccgggc ctctgcccgc
     3421 caacgtcgag gcggcgaccg ccgccgccgc acgcgacgat gtgatcgtcg tcgtggcggc
     3481 gtgcgaccag cccgacctca ccggagccgt ggtggactcg ctcgtcc
//
LOCUS       DATMFS010000266         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_103621, whole genome shotgun sequence.
ACCESSION   DATMFS010000266 DATMFS010000000
VERSION     DATMFS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103621"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VNQ33_04905"
     CDS             <1..571
                     /locus_tag="VNQ33_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWJ97475.1"
                     /translation="PLFATGYDPASPVTTPDGLAEPDELAGDTVVAALAKAVHLLDQA
                     GIPIDAPLGEVQWAQRGEHRVPVHGGYEGEGVLNILAPFGALNSLSLEPDPAPLPAVA
                     PERTMTSGIAAGGYRVTYGTSFLMAVELTDHGPQGIGLLAYGQSGDDRSPHHVDGTEA
                     YAAKATRPLLFTDEAIDADPDLVRRTLRG"
     gene            complement(817..1716)
                     /locus_tag="VNQ33_04910"
     CDS             complement(817..1716)
                     /locus_tag="VNQ33_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWJ97476.1"
                     /translation="MTTNLGGSNAPGRARGYNGLAIASLVLGIIWLGGLGALLAVVLG
                     LVGLSQIKRSGDKGRGVAIAGVVLGAIGLLVPITAVGILIASNRDDPPELSVDNGSSD
                     TSQTTTGTVPASLDGTWSVRTGGDTTAGFRIEESFASGLTDHTAVGRSDDVSGSITVD
                     GTEITEAAFTVGLDSLEFTDDPGLPVGNRANALKQNGLETDTFPEASFELTEPIDFGA
                     KPAEGETVTASATGDLTIHGVTKSVTFDVEAKIVGDTIRVATAEPVPVALADYGIDKP
                     TMGPVASIADAGSFEFLVVLGQE"
     gene            complement(1789..3162)
                     /locus_tag="VNQ33_04915"
     CDS             complement(1789..3162)
                     /locus_tag="VNQ33_04915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ97477.1"
                     /translation="MGTPATLDASLSTRLAAAGASADLETESGIQRVRGWCLVLALVQ
                     TALYPGQFWYLAWGAMAVLFLSWVWVRWALQPESKTPVALVGIIAMATDSIVVIMIMS
                     NLMTSSDDPIQLLPLALAVEAAVRWARVGGVVGGICGGLLLTGWSLGTHVRNDLHFSV
                     GYAAFRFGVVVLVASIVGNAVREMRQQRRAAEAVFQASRDLMLTFSLDGSVLSANPAS
                     EAVLGFTADELRSRDRSFLLEPEDGRDSVALLTHTDGPQRVVRRIQHKDGHLVWIELD
                     LVPDVQAGMVYAIGRDVSDRSRTESELRHRVDHDGLTGAWNRHALVAYLHRMLNRGYL
                     PGLVFIDLDRFKEINDLHGHLTGDGVIIELADRLAGAAGHEGSVARYAGDEFCIVVDD
                     PDDLDLIAERTRRAFDGSFDVNGHTLVVSGSVGSAAARSGDTPESLVHRADQAMYQVK
                     KATRLAL"
     gene            complement(3241..>3451)
                     /locus_tag="VNQ33_04920"
     CDS             complement(3241..>3451)
                     /locus_tag="VNQ33_04920"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HWJ97478.1"
                     /translation="VDLSSSFGDIRSGDQILALGQIVYTLTGQPGIGGVRFTVEGEDV
                     TVPLTDGTLSDDPLSRDDFKAIAPR"
BASE COUNT          527 a         1209 c         1234 g          481 t
ORIGIN      
        1 cccgctgttc gccaccggct acgaccccgc atcaccggtg accacgcccg acgggctggc
       61 cgagcccgac gagctggccg gcgacacggt ggtcgccgcc ctggccaagg cggtccacct
      121 gctcgaccag gccgggatcc cgatcgatgc accactcggc gaggtgcagt gggcccagcg
      181 cggcgagcac cgggtccccg tgcacggggg ctacgagggc gaaggcgtcc tcaacatcct
      241 cgccccgttc ggtgccctca actcgctgag cctcgaaccg gaccctgctc cgctgccagc
      301 cgtggccccg gagcgcacga tgaccagcgg catcgccgcc ggcggctacc gggtcaccta
      361 cgggaccagc ttcttgatgg cggtcgagct caccgaccac ggcccgcagg gcatcgggct
      421 gctcgcctac ggccagtccg gcgacgaccg ctcgccgcat cacgtcgacg gcaccgaggc
      481 ctacgccgcc aaggcgaccc gccccctcct gttcaccgac gaggccatcg acgccgaccc
      541 cgacctggtg cgcaggaccc tgcggggctg acctcgacga ccggctcctg ccgggtcgcg
      601 ggttccaccg ccgccgtcgt cggtcgctgc tcgtgcacct gggcccgccc cagccgaggt
      661 tggtgtgaat tctgctgtcc tgcctcagaa ctcacaccaa cttcgttcgg ggggggcagg
      721 acggacggac cgtgagctcg gctgtccggg cagaccatgc ccgccgccca gggtgacggg
      781 ccggccgggg tgggagccag gatcaggctg gccgagctac tcctggccca gcaccacgag
      841 gaactcgaag gagccggcgt cggcgatgga ggcgaccggg cccatcgtcg gcttgtcgat
      901 gccgtagtcg gcgagggcga ccgggaccgg ctcggcagtg gcgacccgga tggtgtcgcc
      961 cacgatcttg gcttcgacgt cgaacgtcac cgacttggtg acgccgtgga tggtgaggtc
     1021 gcccgtggcc gacgcggtga cggtctcgcc ctcggccggc ttcgcaccga agtcgatggg
     1081 ctcggtgagc tcgaagctgg cctcggggaa cgtgtccgtc tccaggccgt tctgcttgag
     1141 ggcgttggcc cggttgccga ccggcaggcc cgggtcgtcg gtgaactcga gcgagtcgag
     1201 gccgacggtg aacgccgcct cggtgatctc ggtgccgtcg acggtgatcg agccgctgac
     1261 gtcgtccgag cggcccaccg cggtgtggtc ggtgagaccg ctggcgaagg actcctcgat
     1321 gcggaacccc gccgtggtgt cgccacccgt gcgcaccgac caggtcccgt cgagcgacgc
     1381 cggcaccgtg cccgtggtgg tctggctggt gtcggaggag ccgttgtcga ccgacagctc
     1441 cggcgggtcg tcgcggttgg acgcgatcag gatcccgaca gcggtgatcg gcaccagcag
     1501 accgatcgcg ccgaggacga cgccggcgat cgcaacgccg cgccccttgt ccccggagcg
     1561 cttgatctgc gacaggccga cgagcccgag caccaccgcg agcagcgcgc cgagtccacc
     1621 cagccagatg atgccgagca ccagggaggc gatcgccagc ccgttgtagc cgcgcgcacg
     1681 cccgggcgcg ttcgatccac caaggttcgt cgtcatcgga ccagtgtggc cggggcggcc
     1741 gggcgagaga gcggatcccg gcagattccc tgggagctct caggattcct acagggcgag
     1801 gcgggtggcc ttcttgacct ggtacatcgc ctggtccgcc cggtggacga ggctctcggg
     1861 cgtgtcgccg gatcgagcgg cggcgctgcc gaccgagccg gacaccacca gggtgtggcc
     1921 gttcacgtcg aacgacccat cgaacgcgcg gcgggtgcgc tcggcgatca ggtcgaggtc
     1981 atcggggtcg tcgaccacga tgcagaactc gtcgccggca tagcgggcga ccgagccctc
     2041 gtggcctgcc gcgccggcca agcggtcggc cagctcgatg atcacgccgt cgccggtgag
     2101 gtggccgtgg aggtcgttga tctccttgaa ccggtccagg tcgatgaaca ccagtcccgg
     2161 caggtagccg cggttgagca tgcggtgcag gtaggcgacc agcgcatggc ggttccaggc
     2221 accggtgaga ccgtcgtggt ccacgcggtg gcgcagctcg gactcggtgc ggctgcggtc
     2281 gctgacgtcg cgcccgatcg cgtagaccat cccggcctgc acgtcgggca ccaggtcgag
     2341 ctcgatccac acgaggtggc cgtctttgtg ctggatgcgc cgcacgacgc gctgcgggcc
     2401 gtcggtgtgg gtgagcagcg cgaccgagtc ccggccgtcc tcgggctcca gcaggaacga
     2461 gcggtcccgc gaccgcagct cgtcggccgt gaacccgaga accgcctcgg aggccgggtt
     2521 ggccgacagc acggatccgt cgagcgagaa ggtgagcatc aggtcgcgcg acgcctggaa
     2581 cacggcttcg gcggcgcgcc gctgctggcg catctcccgc acggcattgc ccacgatcga
     2641 ggcgaccagc acgaccactc cgaagcgaaa ggcggcgtac ccgaccgaga agtggaggtc
     2701 gttgcggacg tgggtcccga gggaccagcc ggtgagcagc agcccaccgc agatgccgcc
     2761 gaccacgccg ccgacgcggg cccaccgcac cgcggcctcg accgcaagcg ccaggggaag
     2821 cagctggatc gggtcgtccg agctggtcat gaggttgctc atgatcatga tgacgacgat
     2881 cgagtcggtc gccatggcga tgatgcccac cagcgcgacc ggcgtcttcg attccggctg
     2941 gagcgcccag cggacccaga cccagctcaa gaacaggacc gccatggcgc cccaggcgag
     3001 gtaccagaac tggcccgggt agagcgcggt ctgcaccagc gcgaggacga ggcaccagcc
     3061 ccgcacgcgc tggatgccgg actcggtctc gagatccgcg gacgcccctg ctgcagccag
     3121 gcgagtgctg aggctcgcat cgagcgtcgc gggggttccc atccctgttc catcggcagg
     3181 acctgggacg accttcagga tggtcgggaa atcacccgaa caaggccctg accagcggtg
     3241 tcaccggggt gcgatggcct tgaagtcgtc gcgcgaaagc ggatcgtcgc tgagcgtgcc
     3301 gtcggtcagg ggcacggtca catcctcgcc ctccacggtg aagcgcaccc cgccgatgcc
     3361 cggctgcccg gtgagcgtgt agacgatctg gcccagcgcc aggatctggt cgccgctgcg
     3421 gatgtcgccg aacgacgacg agaggtcgac g
//
LOCUS       DATMFS010000267         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_79477, whole genome shotgun sequence.
ACCESSION   DATMFS010000267 DATMFS010000000
VERSION     DATMFS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79477"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VNQ33_04925"
     CDS             complement(<1..249)
                     /locus_tag="VNQ33_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97479.1"
                     /translation="MIAIQATDRAAKAHVGWSPVPPATNDLVSRLVAAGMIAPVPITD
                     QGPSRVEQVSSRRSLVLGLSGVTMVVEVLSIAGLVPKLW"
     gene            complement(246..1229)
                     /locus_tag="VNQ33_04930"
     CDS             complement(246..1229)
                     /locus_tag="VNQ33_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWJ97480.1"
                     /translation="MSKAEAIDTSVGPIGGQALPEGVMMRRGQSWGAAVRRQDGTIGT
                     TSRVLPASLDTWRRLPLVRGVMALGETAALGTKATVWAAQERGDESGDGYTRKGLAAT
                     IAIAIIAVVGIFGLLPAVLVKLAGVDGAVTFPLAEGLIRLGILVGYLWLLGRSPQVQR
                     VFAYHGAEHMTIHAFEHRIALVPAEIRHFSRRHPRCGTAFLLVVVITTMFVHAFMGHH
                     SWPWLVAGRILGLPLVAGLAYEAIRFAGRHQHRWYGRIIMAPGSWLQGITTAEPTDDM
                     IEVAVAALEATLAAEPLAPASDDLTAEAIGAATTATTTLAAGPTATAGAAR"
     gene            complement(1499..1930)
                     /locus_tag="VNQ33_04935"
     CDS             complement(1499..1930)
                     /locus_tag="VNQ33_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97481.1"
                     /translation="MPDNGAARTTVVVANEEPTVCEVLARVVEADGYEAVRVTDPSQV
                     VGGVLSAPALALVLDLEADNVHQLRALRGGDHPRSTSSRAVVIASGPANALLAWQADA
                     DAVLTRPFRAEELQAAITEVLAREPLDRATVRRTHIESLTA"
     gene            complement(1941..>3132)
                     /locus_tag="VNQ33_04940"
     CDS             complement(1941..>3132)
                     /locus_tag="VNQ33_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWJ97482.1"
                     /translation="PAHATGKPLDPREIVLKTGEVMAASGTPAVSPPISTVPDIIIES
                     NSVFDRITPEEVWSCTSCKACDEICPVNIEILDKILDMRRYLTLMEASFPSELGSAFN
                     GMENQGNPWGMSQSDRAAWTAKVDDVAIVDGSEAFDHEFLYWVGCAGSFDDKNQKVTQ
                     AMAKLMARAEIDFAILGPSENCTGDSARRSGNEYIFQMLAMQNIEMMNGMGVKKIITQ
                     CPHCFNTLMNEYPQLGGNYEVVHHSQFLEWLIDQGRLDLSEAKLEERVVYHDSCYLGR
                     HNDVYMAPRNVIGSLAGIEIVEASANGTKGMCCGAGGARMWMEETIGTKVNDARSEQL
                     LDTGAERVATACPFCYIMIDDGVKGLGKGDDVQVADIAMHLLEALEAGEAKGIGGVLE
                     TIED"
BASE COUNT          446 a         1021 c         1174 g          491 t
ORIGIN      
        1 ccagagcttc gggaccagcc cggcgatgct cagcacctcg acgaccatgg tgacgcccga
       61 cagcccgagc accaggctgc gccggctgct cacctgctcg acccggctgg gtccctggtc
      121 cgtgatgggc acgggggcga tcatgcccgc tgcgaccagc cgggacacga ggtcgttggt
      181 cgccggcggg accggcgacc aaccgacgtg cgccttggcg gcgcggtcgg tggcctggat
      241 ggcgatcacc gggctgctcc ggcggtggcg gtgggcccgg cggcgagggt ggtggtggcg
      301 gtggtggcgg cgccgatggc ctcggcggtg aggtcgtcgg acgcgggcgc gagcggttcg
      361 gcggcgaggg tcgcctcgag ggcggcgacg gccacctcga tcatgtcgtc ggtgggctcg
      421 gccgtcgtga tcccctgcag ccaggagccc ggcgccatga tgatccgccc gtaccagcgg
      481 tgctggtggc ggccggcgaa gcggatggct tcgtaggcga ggccggccac gagcggcagt
      541 ccgaggatgc ggccggcgac cagccacggc cagctgtggt ggcccatgaa ggcgtggacg
      601 aacatcgtcg tgatcacgac gaccagcagg aacgccgtcc cgcagcgtgg gtggcggcgg
      661 ctgaagtggc ggatctcggc gggcacgagg gcgatgcggt gctcgaaggc gtggatcgtc
      721 atgtgctcgg caccgtggta ggcgaacacc cgctgcacct ggggcgaccg gccgagcagc
      781 cagaggtacc cgacgaggat gccgaggcgg atgaggccct cggccagcgg gaaggtcacg
      841 gcgccgtcga cgccggcgag cttcacgagc acggcgggca gcaggccgaa gatcccgacc
      901 acggcgatga tggcgatggc gatggtggcg gccaggccct tgcgggtgta cccgtcgcct
      961 gactcgtcac cccgctcctg agccgcccag acggtggcct tggtgccgag cgccgcggtc
     1021 tcgccgagcg ccatgacgcc ccggaccagt ggcagccggc gccacgtgtc gaggctggcc
     1081 ggcaggaccc ggctggtggt gccgatcgtc ccgtcctggc ggcggacggc cgctccccag
     1141 gactggcccc gtcgcatcat cacgccttcg ggcagcgcct gcccgccgat cggtccgacg
     1201 ctcgtgtcga tcgcttcagc cttgctcacg ttcggtgccc ctccagctgc tgaccacgga
     1261 gagcgatcag ctgatgagca aatactacgc acagtggcag cacttgtcta ggccaaatga
     1321 cctagttcgg accgtgctca tcccctcacc gaacgtgatc ctcccaccga tccaccccac
     1381 ccgctaccgg caagcagcac cccaccgaag ttggtgtgaa aaccgaggca ggagcgcagt
     1441 tcgcacacca agttcggtca ccaagttcgg tcgtggaacg ggtcgggccg aacccgggtc
     1501 aggcggtgag ggactcgatg tgggtgcggc ggaccgtggc ccggtcgagg ggctcgcggg
     1561 cgaggacctc ggtgatggcg gcctgcagct cctcggcccg gaacgggcgg gtcagcacgg
     1621 cgtcggcgtc ggcctgccag gcgagcaggg cgttggccgg gcccgaggcg atcacgacgg
     1681 cccgcgagct ggtggagcgg ggatggtcgc cgccgcgcag ggcccggagc tggtggacgt
     1741 tgtccgcctc gaggtcgagc accagggcca gggccggggc gctgagcacg ccaccgacga
     1801 cctggctcgg atcggtgacg cggacggcct cgtagccgtc ggcctcgacg acgcgggcga
     1861 gcacctcgca gaccgtgggt tcctcgttgg ccaccacgac ggtggtccgt gctgcgccgt
     1921 tgtcgggcaa ggtcggcgcc tcagtcctcg atggtctcga gcacgccgcc gatgcccttg
     1981 gcctcgccgg cctcgagcgc ctcgaggagg tgcatggcga tgtcggcgac ctggacgtcg
     2041 tcgcccttgc cgaggccctt cacgccgtcg tcgatcatga tgtagcagaa ggggcaggcg
     2101 gtggcgacgc gctcggcgcc cgtgtcgagc agctgctcgg agcgggcgtc gttgaccttg
     2161 gtgccgatgg tctcctccat ccacatgcgg gcgccgccgg cgccgcagca catgcccttg
     2221 gtcccgttgg ccgaggcctc cacgatctcg atgcccgcca gcgagccgat gacgttgcgg
     2281 ggggccatgt agacgtcgtt gtggcggccc aggtagcagg agtcgtggta gacgacccgc
     2341 tcctcgagct tggcctcgct gaggtcgagg cggccctggt cgatcagcca ctcgaggaac
     2401 tgcgagtggt gcacgacctc gtagttgccg ccgagctgcg ggtactcgtt catcagcgtg
     2461 ttgaagcagt gcgggcactg cgtgatgatc ttcttcacgc ccatgccgtt catcatctcg
     2521 atgttctgca tggccagcat ctggaagatg tactcgttgc cggaccggcg ggcggagtcg
     2581 ccggtgcagt tctccgacgg gccgaggatg gcgaagtcga tctcggcgcg cgccatgagc
     2641 ttcgccatgg cctgggtgac cttctggttc ttgtcgtcga aggacccggc gcagccgacc
     2701 cagtagagga actcgtggtc gaaggcctcg gagccgtcga cgatcgccac gtcgtcgacc
     2761 ttggcggtcc aggcggcccg gtcggactgc gacatgcccc acgggttgcc ctggttctcc
     2821 atgccgttga aggcggaacc gagctcgctc gggaagctgg cctccatgag cgtgaggtag
     2881 cggcgcatgt cgaggatctt gtcgaggatc tcgatgttga ccgggcagat ctcgtcgcac
     2941 gccttgcagc tggtgcagga ccagacctct tcgggcgtga tccggtcgaa cacggagttg
     3001 gactcgatga tgatgtcggg caccgtgctg atggggggcg acacggcggg ggtgccggag
     3061 gcggccatga cctcgccggt cttgagcacg atctcgcggg ggtcgagcgg cttgccggtg
     3121 gcgtgggccg gg
//
LOCUS       DATMFS010000268         4714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_312303, whole genome shotgun sequence.
ACCESSION   DATMFS010000268 DATMFS010000000
VERSION     DATMFS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312303"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VNQ33_04945"
     CDS             complement(<1..382)
                     /locus_tag="VNQ33_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HWJ97483.1"
                     /translation="MADGEHLWTSCQESLRTQVSDAVWRSTFQDITEIDVEDDILRLA
                     VPSAMVKDRVEGRYLSLVEDIVLDQSGQRLSILLEVRPNAGESSTVSDPLDERIGARP
                     AEVRTDGGAVSDNGLADNRHAPPSL"
     gene            765..899
                     /gene="rpmH"
                     /locus_tag="VNQ33_04950"
     CDS             765..899
                     /gene="rpmH"
                     /locus_tag="VNQ33_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWJ97484.1"
                     /translation="MKRTYQPNVRKRAKNHGFRKRMSTKAGRAVLRNRRLKGRAKLSA
                     "
     gene            899..1255
                     /gene="rnpA"
                     /locus_tag="VNQ33_04955"
     CDS             899..1255
                     /gene="rnpA"
                     /locus_tag="VNQ33_04955"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWJ97485.1"
                     /translation="MRPVRDRRTFVALRARGIRVRRGPLALTFLAEGEADTTRVAYAI
                     TKRVGGAVERNRLRRRLRAVFVDLAREAPGSVPAGVLLVSAGPDVSGRDPEELRNDVK
                     RLLAALETRRFGGEAP"
     gene            1270..1527
                     /gene="yidD"
                     /locus_tag="VNQ33_04960"
     CDS             1270..1527
                     /gene="yidD"
                     /locus_tag="VNQ33_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWJ97486.1"
                     /translation="MIRRLVLFYQHLRAGRPSPCRFQPSCSSYAVEALEVHGAVRGSW
                     LAIRRLSRCHPWGGQGWDPVPEPTDRHPGHRHDHHHERTVV"
     gene            1527..2636
                     /locus_tag="VNQ33_04965"
     CDS             1527..2636
                     /locus_tag="VNQ33_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HWJ97487.1"
                     /translation="MYVFIAKPLEFFYGLYPNYAVAIALLTLSIMILLLPLTLKGTRS
                     MLAMQKLQPELKKIQNKYKDDRQKLNEEMMAFYKENNINPVSGCLPLLLQMPVFIVLY
                     RTLFQLLNRAPYGYDMGAAAVRATTNVNGGIWERFGFFQPQHISHTSQLYIDLSNTRV
                     MEAWGMNLAESAQKALREGVGHAAAYIVLVLLVTATSYYQQKQVSGRNTQAQVNPQQQ
                     MLMRIMPLFFAFISFTLPAGIVVYFLVSNLFRVGQQALITRTMYRDDSGPLATTGRVS
                     DKSSNKSSADAEKPKGFFAQMKEIGLPNPGAAGDDAKASSSSSGAAKANAKAKAGSGS
                     SGSKGSTTTSTTSTAGPSRAAPSAANRSKSKKKRR"
     gene            2643..3536
                     /gene="jag"
                     /locus_tag="VNQ33_04970"
     CDS             2643..3536
                     /gene="jag"
                     /locus_tag="VNQ33_04970"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HWJ97488.1"
                     /translation="MQWVETTGRTIEDAKEAALDQLGIDESEAEFEVVEEPKTGLFGR
                     PRGEARIRARVVPKAPRPKQERRPRRQKGGGKDRSSEPAAAGAESDEDASAGDADAGT
                     SQGGGNRRGGGRKRSGSNGGGRPAKNQQEGNQMTDEEVPVSDQADIITEFLEGLLDSL
                     GAEGTVTRTVVDDETIELGVEGDDLGLLIGPKGQTLTAVHELSRTVLQRRATGRHVGR
                     VRIDIGGYRQRRQEALARFVRQQAEAVLSEGAARALEPMNSADRKVVHDTVNEIAGVE
                     TVSEGADPDRRVVIVPEGASA"
     gene            3533..4159
                     /locus_tag="VNQ33_04975"
     CDS             3533..4159
                     /locus_tag="VNQ33_04975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HWJ97489.1"
                     /translation="MTLELTGLLEEARQRGFLGPGPVEPHLDHARAFAVAAGEAPARA
                     VDLGAGGGLPGLVLAHEVWPGTAWTFVDAQRKRTDFLAEAVEALGLDDRVVVVTERAE
                     IIGRDAEHRRTYDLVVSRSFGRPAVVAECAAPLLRPGGRLVVSEPPATNVSDRWPTAP
                     LATLGCAPAQAQVIGEGEGYHFVRIDQAEVTADRFPRRVGIPTKRPLF"
BASE COUNT          726 a         1632 c         1657 g          699 t
ORIGIN      
        1 cgaggctcgg gggtgcgtgc cggttgtcgg cgaggccgtt gtcggagacc gcgccgccat
       61 cggtcctgac ctcggcgggc cgtgctccga tgcgctcatc caacgggtcg gagacggtgg
      121 agctctcgcc ggcgttggga cggacctcga gcaggatcga gagacgctgt ccagactggt
      181 cgagcacgat gtcttcgacc agggagaggt agcggccctc gacgcggtct ttgaccatgg
      241 cgctgggcac ggcgaggcgg aggatgtcgt cttcgacgtc gatctcggtg atgtcctgga
      301 acgtcgaacg ccagaccgca tcggacacct gggtccggag ggattcctgg cagctcgtcc
      361 acaggtgttc tccgtcagcc acctggtcga accgtccttt tcctcggcgt tccacagcgg
      421 ggtccacacc tgtggaaaac atggcgtacc cggaggcggc tcgggcgctt ccgggggtga
      481 gaagatctcg ccgtcggcgg cctcgggaac ccgagggacc gcgctggcag gggccggacc
      541 gtagcaccgt ccgcgggccg ccgcgcaagg gtgggtccgg cgtcggcacc accgccggtc
      601 cggcaccagg tggcggccgc gattgccccg tggtggcacg gggacgtagc gttgcccgga
      661 tgcctccgcg cgcctgcgcc gaggtgcacc gtcgattcga tgcggccgcg ttcttggtgg
      721 ccgcccgagc tgccacccgt gcggctcgtg aggaggagag agcagtgaag cgcacttacc
      781 agcccaacgt ccgcaagcgg gccaagaacc acggcttccg caagcggatg tccaccaagg
      841 cgggccgcgc cgtgctgcgg aaccgccgcc tgaagggccg ggcgaagctg tcggcctgat
      901 ccgtcccgtc cgtgaccgcc gcaccttcgt ggcgttgcgg gcgcgcggga tccgcgtgcg
      961 acgcgggccg ctcgccctca ccttcctcgc ggaaggggag gcggacacca cccgggtcgc
     1021 ctacgcgatc accaagcggg tgggcggtgc cgtggagcgg aaccggcttc gtcgtcggct
     1081 ccgagccgtg ttcgtcgatc tggctcgcga ggcgccgggc tcggtccccg ctggtgtgct
     1141 gctggtctcc gccggcccgg acgtgtccgg ccgagacccc gaggaactga ggaacgacgt
     1201 gaagaggctg ctcgccgcac tcgagacccg ccggttcggc ggagaggcgc cgtgagcacc
     1261 cccgcgcgca tgatccggcg tctcgtgctg ttctaccagc accttcgcgc cgggcggccc
     1321 tccccttgcc gcttccaacc gagctgctcg tcgtacgccg tcgaggcgct cgaggtgcac
     1381 ggggccgtac gcggctcctg gctcgccatc cgccgcttga gccggtgcca cccttggggc
     1441 gggcaaggtt gggatccggt acccgaaccc actgatcgtc accccggtca ccgccacgat
     1501 caccaccacg aacggacggt cgtctgatgt acgtgttcat cgcgaagccg ctggagttct
     1561 tctacggcct ctaccccaac tacgcggtcg ccatcgcgct gttgacgctg tcgatcatga
     1621 tcctgctgct cccgctgacc ctgaagggca cgcggagcat gctggcgatg cagaagctcc
     1681 agcccgagct caagaagatc cagaacaagt acaaggacga tcgccagaag ctcaacgaag
     1741 agatgatggc gttctacaag gagaacaaca tcaacccggt ctcgggttgc ttgccgctcc
     1801 tgctgcagat gccggtgttc atcgtcttgt accggaccct cttccagctg ctgaaccggg
     1861 cgccctacgg ctacgacatg ggtgccgccg ccgtccgggc caccaccaac gtgaacggtg
     1921 gcatctggga gcggttcggc ttcttccagc cccagcacat ctcccacacc tcgcagctct
     1981 acatcgacct cagcaacacc cgggtcatgg aggcctgggg catgaacctc gccgagtcgg
     2041 cccagaaggc gctgcgcgag ggtgtcggcc atgcggcggc gtacatcgtc ctcgtcctgc
     2101 tggtgacggc gacctcgtac taccagcaga agcaggtctc gggtcggaac acgcaggcac
     2161 aggtcaaccc acagcagcag atgctgatgc ggatcatgcc gctgttcttc gccttcatct
     2221 cgttcaccct gcccgccggc atcgtcgtct acttcctcgt gtcgaacctg ttccgggtcg
     2281 gccagcaggc cctgatcacc cggaccatgt accgcgacga cagcggaccc ctggccacca
     2341 ccgggcgagt tagtgacaaa tcgtcaaaca aatcctctgc tgatgcagag aagcccaagg
     2401 gtttcttcgc ccagatgaag gagatcgggc tgccgaaccc gggggccgcg ggcgacgacg
     2461 ccaaggcgtc gtcctcgtcg agcggggccg ccaaggccaa cgccaaggcc aaggccggat
     2521 ctggatcatc gggatcgaag ggatccacca ccacgtcgac cacgtcgacg gctggaccga
     2581 gccgagccgc accatccgcg gccaaccggt ccaaatccaa gaagaagagg aggtgatcgc
     2641 tcgtgcaatg ggttgagacc accgggcgca ccatcgagga cgccaaggaa gccgcgctgg
     2701 accagctcgg catcgacgag agcgaagcgg agttcgaggt cgtcgaggag cccaagaccg
     2761 ggctcttcgg ccgcccccgc ggcgaggccc gcatccgggc ccgcgtcgtg ccgaaggccc
     2821 cccggcccaa gcaggagcgc cggccgcgcc ggcaaaaggg cggcggcaag gaccgcagca
     2881 gcgaacccgc ggcggcgggc gccgagtcgg atgaggacgc ctccgccggt gatgccgacg
     2941 ccggtaccag ccaaggcggt ggcaaccgtc gaggcggcgg tcgcaagcgc tctggatcga
     3001 acggtggcgg ccgtcccgcc aagaaccagc aggaaggcaa tcagatgacc gacgaagagg
     3061 tcccggtcag cgatcaagct gacatcatca ccgagttcct cgaaggcctg ctcgattcgc
     3121 tgggcgccga gggaacggtc acgcgcaccg tggtcgacga cgagaccatc gagctcgggg
     3181 tggagggcga cgatctcggc ctgctgatcg gcccgaaggg ccagacgctc accgcggtgc
     3241 acgagctgtc ccgcacggtc ctgcagcgcc gggccaccgg gcgccacgtc ggccgcgttc
     3301 ggatcgacat cggcggctac cgccagcggc gccaggaagc cctcgcccgc ttcgtgcgcc
     3361 agcaggccga ggcggtcttg agcgagggcg cggcccgggc gctcgagccg atgaactcgg
     3421 cggaccgcaa ggtggtccac gacacggtga acgagatcgc cggcgtcgag acggtgtccg
     3481 aaggcgccga ccccgaccgc cgcgtggtga tcgtccccga gggcgccagc gcgtgaccct
     3541 cgagctgacc ggcctcctgg aggaggcccg ccagcgcggt ttcctgggcc cgggcccggt
     3601 cgagccacac ctcgaccacg cccgggcctt tgccgttgct gcgggcgaag cccctgcccg
     3661 tgccgtcgac ctgggcgcgg ggggtggcct tcccggcctg gtcctcgccc acgaggtctg
     3721 gccgggcacc gcctggacgt tcgtggatgc ccagcgcaag cgcaccgact tcctcgccga
     3781 ggcggtcgag gcgctgggcc tcgatgaccg cgtcgtggtc gtcaccgagc gggcggagat
     3841 catcggccga gacgccgagc atcgtcgcac ctacgacctg gtggtgtctc ggagcttcgg
     3901 ccggcccgcg gtcgtagccg agtgcgccgc tcccctgctg cggcccggtg gccggctcgt
     3961 ggtgagcgag ccgcccgcca ccaacgtgtc cgatcggtgg ccgacggcgc cactggccac
     4021 gctcgggtgc gctccggcgc aagcccaggt catcggggag ggcgagggct accacttcgt
     4081 ccgcatcgac caggctgagg tcaccgccga tcggtttccc cggcgcgtgg ggatccccac
     4141 caagcgcccg ctcttttgac cggacgctcc cggggcgtgc aggccctggc gcctggtgtc
     4201 cgctggctgg cgctcgatct ggtccatgtc ggtgctctcc ggtgtggcgc gggcagcgct
     4261 gctcgggctc gggcgcctca cccgagccca agaacgtccg cacgagccac tcgtcgatga
     4321 ccggcgaaga cccggtgccg gctgttgtgt tcgggagggc ctcgggaacg gggcggcagg
     4381 agctcatggc ctcggtcggt cgttccgcca tcggtgaccg ggccgcaggg gtcggtggtg
     4441 gatgcgagcg ggttcttggc gctcgcggtg ccacccgcgc tgaggccggc gacgtcctga
     4501 cctgcgcatc gtccgccgaa taggccgaca gaaccgggcg tcccgggtcg gtggtcgccc
     4561 gcagtccgtc gggagttgga gagggcgccg acgtgggccg tcggcagggt gggagcgcgt
     4621 gggggccctg tcgaacgggt gcccacttcg cttgaccacc gtttcacgtg aaacaggcgg
     4681 gggactggca gaacggctcg tccactgcgg cgtt
//
LOCUS       DATMFS010000269         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_126112, whole genome shotgun sequence.
ACCESSION   DATMFS010000269 DATMFS010000000
VERSION     DATMFS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126112"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VNQ33_04980"
     CDS             <1..293
                     /locus_tag="VNQ33_04980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWJ97490.1"
                     /translation="REVFEEVGLVVDLVGEPRVVIDADPQRVDVVYRAKVPSGVDPAM
                     ASPQSPEILETRWFPRDALPELQHETVTALLALARPGPAVSAPPIGVESLDR"
     gene            412..831
                     /locus_tag="VNQ33_04985"
     CDS             412..831
                     /locus_tag="VNQ33_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ97491.1"
                     /translation="MPLPRPATRSPWASLSRPGGAWLPRAKRRAEARRHLERAVEVFG
                     FAGATGLRRRAEDELAGGSLDRNRPADELLNPTEVHVARLAVTGLTNRDIAGQLSMSP
                     RTVENHVGAVYRKIGVAGRTGLTAGSLSDPVLRAVPG"
     gene            complement(881..1576)
                     /locus_tag="VNQ33_04990"
     CDS             complement(881..1576)
                     /locus_tag="VNQ33_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97492.1"
                     /translation="MAIGSSIDGRDAVLRERLRSLSDLPLLSDGEELVAAATIADGRD
                     AENELAALGSQPNIDLTTAAELMERVRRGEAARDLLVGSTRRLVVSLARRHPSTPEVT
                     LALLDAGDEAVGRAADRYDPTGGLPFSSFARWWVERAMREVDRHPSQGRAENPSPADP
                     VLLTALGHLHREDTRVVELRLGLHGGPALSDQDTARVLGIDTDAEHEREQRAVAKLRH
                     PCTPGDLTHLRSL"
     gene            1795..1929
                     /locus_tag="VNQ33_04995"
     CDS             1795..1929
                     /locus_tag="VNQ33_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97493.1"
                     /translation="MKAFVGRYSQSSEPTALGRTDPSCSTLQTLESWIYLVSTDPLGS
                     "
     gene            2342..>3382
                     /locus_tag="VNQ33_05000"
     CDS             2342..>3382
                     /locus_tag="VNQ33_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ97494.1"
                     /translation="MTDVGVGRPAVFDPFEDGFVDDPYDQYARLRAGDPVHRSALLHG
                     WVVTRFDDVSKLLRDRSISSEITKATPNPLTIMELERLAERSRASETIVLMDDPDHAR
                     VRKLMSEPFRPSEIERLRQRVEARIEGALDQLREERGSGVIELDLTAEFAYPLPVEIF
                     SEMLGVPDEDHARFRYLSQQVAKTVDPVMSGSERDECMAALDEMHEYLTAQAARKRAE
                     PADDLMSALVHAADDDGVSFTDSELIAQLVTLYLAGHEPVASLLSAGTLALLRHPDQL
                     AKVQANRSLVRNTVHELLRYDGPNHFMRRITTQPTVVGDVELPAGEVIYASPAAANRD
                     PDRWGGTADQVVV"
BASE COUNT          455 a         1221 c         1236 g          470 t
ORIGIN      
        1 gccgcgaggt cttcgaggag gtcggcctgg tggtcgatct ggtcggcgag ccgcgggtgg
       61 tcatcgacgc ggacccgcag cgggtggacg tcgtctaccg ggcgaaggtg ccgtcgggcg
      121 tcgaccctgc catggcctcg ccgcagtcgc ccgagatcct cgagacgagg tggtttcccc
      181 gcgacgcgct cccggagctc cagcacgaga ccgtcacggc gctgctcgcc ctcgcccggc
      241 ccggcccggc ggtgtcggcg ccaccgatcg gggtcgagtc gctcgaccgg tagcgttgcc
      301 ggacggcgaa ccaccccgac cgctcgccct cgctgtgtct ctcgcacccc ggcaacctgg
      361 tctgtccgcg atcttcggcc gttcggggcg ggcacttcga ggccgcccgc gatgcctttg
      421 cctcgaccgg caacaagatc gccctgggca tcgctgagtc gacctggggg cgcatggctg
      481 ccgcgcgcca agcgccgcgc cgaggcccgc cgccacctgg agcgtgccgt cgaggtgttc
      541 gggttcgccg gcgccaccgg tctccgtcgg cgggccgagg acgagctggc cggtgggtcg
      601 ctcgaccgga accggccggc ggacgaactg ctcaatccca ccgaggtgca cgtcgcccgc
      661 ctcgccgtca ccggtctcac caaccgcgac atcgccggtc agctgtccat gagccccagg
      721 acggtcgaga accacgtggg cgccgtctac cgcaagatcg gcgtggcggg ccgcaccggt
      781 ctcacggccg gctcgctgtc ggacccggtg ctgcgtgctg tcccgggctg aacgccgacc
      841 cggccgctcg gtgcagccgg tcggcgacgg gtcgcgccgg tcagagcgag cgcaggtggg
      901 tgagatcgcc cggggtgcag gggtggcgca gcttggcgac cgcccgctgc tcgcgctcgt
      961 gctcggcatc ggtgtcgatg ccgagcaccc gtgcggtgtc ctggtcggag agggcggggc
     1021 cgccgtggag gccgaggcgc agctcgacga cgcgcgtgtc ctcgcggtgg aggtggccga
     1081 gcgcggtgag cagcaccggg tctgccggtg acgggttctc ggcacggccc tgcgacgggt
     1141 ggcggtcgac ttcgcgcatg gcccgctcga cccaccagcg ggcgaacgag ctgaagggca
     1201 ggccgccggt ggggtcgtag cggtcggctg ctcggccgac ggcctcgtcg cccgcatcga
     1261 ggagggcgag ggtgacctcg ggcgtgctgg ggtggcggcg ggcgagcgac accacgaggc
     1321 ggcgggtgga tccgaccagc aggtcgcggg ccgcttcgcc ccggcgcacc cgctccatga
     1381 gctcggccgc tgtcgtgagg tcgatgttcg gctggctgcc gagggcggcg agctcgttct
     1441 ccgcatcgcg cccgtcggcg atggtggcgg cggccaccag ctcttcgcca tcggagagca
     1501 gggggaggtc gctcagggag cggaggcgct cgcggaggac cgcgtcacgg ccgtcgatcg
     1561 aggatccgat ggccatcgag ctccttcctc tcgcacccgg gcgacctacc cggtgggaag
     1621 cgaaacggta ccgccggtgc ccacccaggt ggcggatcgt ccacgacgat ctgcgtccgg
     1681 ggcgccacct ggtgcgttca gccccggcaa ggggcgtgga cggccccctc aaccggccgg
     1741 cctcgctcca acccggctcg ggacctcaca ccggtctggg tcgagaacgg cgaggtgaag
     1801 gccttcgtcg gccgctactc gcagagcagc gagccgacgg cgctgggtcg gaccgacccg
     1861 tcgtgcagca cgcttcagac cctcgagtcc tggatctacc tcgtgagcac ggatccgctc
     1921 ggttcctgat cgtggtgccg cccgtggatc gcgccgggcg cgggcggcgc cgcctacccg
     1981 tcctgcgggg agggcaggac gcggcggccg gtggctgacg ggaggagcag ccaccggccg
     2041 gtcgcccgca ccagacgtcg cggtgcaggc tggccgagtg ctgcggctcg cccgtgtccg
     2101 aaagattcgc tgacaccgcc ctcatgttcc tggtagacca gccgatgtcg tgaggggccg
     2161 gcgtgcggag ggcacgccgg cccttcgtca cctccggggc cgagccgcgc cgttcgcccc
     2221 atgtgccggc cggggagtcc cggcaggctg gccccgtgac ctgacggatc gtcagctcac
     2281 gggcaccccg ggtggtagac caccatcgtc cggccaggtg aacggcacag gggaagcagc
     2341 gatgaccgac gtgggtgtgg gcaggccggc ggtgttcgac ccgttcgaag acgggttcgt
     2401 cgacgacccg tacgaccagt acgcgcgcct gcgcgccggc gatccggtgc accgttcggc
     2461 gctgctgcac ggctgggtcg tgacccgctt cgacgacgtg tcgaagctgc tgcgggaccg
     2521 gtcgatctcg agcgagatca ccaaggcgac gcccaacccc ctcaccatca tggagctcga
     2581 gcgcctggcc gagcggtccc gcgcctcgga gacgatcgtg ctcatggacg acccggacca
     2641 cgcccgggtc cgcaagctga tgtccgagcc gttccggccc agcgagatcg agcggctgcg
     2701 acagcgggtg gaggcccgca tcgagggcgc cctcgaccag ctgcgggagg agcgggggag
     2761 cggcgtcatc gagctcgacc tcaccgccga gttcgcctac ccgctgcccg tcgagatctt
     2821 ctccgagatg ctcggcgtgc ctgacgagga ccacgctcgg ttccgctacc tctcgcagca
     2881 ggtggcgaag acggtggacc cggtgatgag cgggtccgaa cgagacgagt gcatggccgc
     2941 gctcgacgag atgcacgagt acctgaccgc ccaagccgcc cgcaagcgcg ccgaaccggc
     3001 cgacgacctg atgtccgcgc tcgtccacgc tgccgacgac gacggcgtct cgttcaccga
     3061 cagcgagctc atcgcccagc tcgtgaccct gtacctggca ggtcacgagc cggtcgcctc
     3121 gctgctcagc gcgggcaccc tcgcgctgct gcgccatccc gaccagctcg ccaaggtgca
     3181 ggccaaccgt tcgctcgtcc gcaacaccgt gcacgagctc ctgcgctacg acggacccaa
     3241 ccacttcatg cgccgcatca ccacccagcc gaccgtggtg ggcgacgtgg agctgccggc
     3301 tggcgaggtc atctatgcca gccccgccgc cgccaaccgc gaccccgacc gctggggcgg
     3361 caccgccgac caggtggtgg tc
//
LOCUS       DATMFS010000270         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_126116, whole genome shotgun sequence.
ACCESSION   DATMFS010000270 DATMFS010000000
VERSION     DATMFS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126116"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VNQ33_05005"
     CDS             <1..1619
                     /locus_tag="VNQ33_05005"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ97495.1"
                     /translation="SMSAPPLGRVASLGRRLPPAARAAAARLPGSLAPPVEPGRPTPR
                     VAIIGSGFGGLGMACRLRQAGIDTFTIYEKADAIGGTWRDNTYPGAACDVPSHLYSLS
                     FAPKTDWTRKFPEQPEILGYLNEVTDRFGLRSHLRLGVEVSAATWDDAGSCWHLELAD
                     GTVADADVVVAATGQLNRPQIPGIAGLDQFGGTVFHSARWDHDHDLAGRDVAVIGIGA
                     SAIQFVPEIARTCGRLTLFQRSSNFVAPKPDGVFSEETRERFRRYPALQRLYRLSIWL
                     RFDARWLLFRKGSSAARLLEKRFTSELAKIVTDELPAKALLPDYPVGCKRILISNDWY
                     PTLMRDNVRVVTDSVERITPAGVVADGVEHPAETVIFGTGFASTGFLAPIRVTGRGGR
                     DLHAEWRDGAEAHLGITVAGFPNLFVLYGPNTNLGHNSIIFMLERQIAYALTWIRRLV
                     EDDATAVEVRRDAQAASNARLQRHLERTVWAASCHSWYKTASGKITNNWSGPTVAYWW
                     RTRRPDRAEFATTPGPGRRPAHSGAAGSSE"
     gene            1696..>2004
                     /locus_tag="VNQ33_05010"
     CDS             1696..>2004
                     /locus_tag="VNQ33_05010"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWJ97496.1"
                     /translation="MTTEPVRVAVTGGAGQIGYSLLFRIASGALLGPDTPVILQLLEI
                     TPALGALDGVAMELEDCAFPLLAGVVKTDDADVAFGDVDIALLVGSRPRTAGMERGDL
                     L"
BASE COUNT          280 a          768 c          661 g          295 t
ORIGIN      
        1 cgtcgatgag cgctcctccc cttggtcgcg tcgcatcgct cggacgtcgg ctgccgccgg
       61 ccgcgcgcgc cgccgcagcc cggctgccgg ggtcgctcgc gccgcccgtc gagcccggcc
      121 ggcccacgcc tcgcgtcgcg atcatcggct cgggcttcgg tgggctcggc atggcgtgcc
      181 gtctgcggca ggcggggatc gacaccttca cgatctacga gaaggccgac gccatcgggg
      241 gcacgtggcg ggacaacacc tatcccgggg ctgcctgcga cgtgccgtcg cacctctact
      301 cgctgtcgtt cgccccgaag accgactgga cccgcaagtt cccggagcag ccggagatcc
      361 tcggctacct gaacgaggtc accgaccgct tcggcctccg gtcgcacctg cggctcggcg
      421 tcgaggtgag cgcggccacc tgggacgacg ccgggtcctg ctggcacctc gagctcgccg
      481 acgggaccgt cgccgatgcc gatgtggtcg tggcggccac cggtcagctc aaccgccccc
      541 agatccccgg catcgccggc ctcgaccagt tcggcggcac cgtcttccac tcagcccgct
      601 gggaccacga tcacgacctg gccgggcgcg acgtcgccgt gatcggcatc ggcgccagtg
      661 ccatccagtt cgtccccgag atcgccagga cctgtggtcg cctgacgctc ttccagcgca
      721 gcagcaactt cgtcgcaccc aagcccgacg gcgtgttctc cgaggagacc cgcgagcggt
      781 tccggcggta cccggcgctc cagcggctct accgcctgtc gatctggctg cggttcgacg
      841 cccggtggct gctgttccgc aagggctcgt ccgccgcccg gctgctcgag aagcgcttca
      901 cgtcggagct ggccaagatc gtgaccgacg agctcccggc gaaggcgctg ctgcccgact
      961 accccgtcgg gtgcaagcgc atcttgatct cgaacgactg gtacccgacc ctcatgcggg
     1021 acaacgtgcg cgttgtgacc gactcggtcg agcgcatcac ccccgctggc gtggtggccg
     1081 acggcgtgga gcacccggcc gagacggtca tcttcggaac cgggttcgcc tcgaccggct
     1141 tcctcgcacc gatccgggtc accggccgcg gcgggcgcga cctgcacgcc gagtggcgcg
     1201 acggcgccga ggcccacctc ggcatcactg tggccggctt cccgaacctg ttcgtgctgt
     1261 acgggccgaa caccaacctc ggccacaact cgatcatctt catgctcgag cgccagatcg
     1321 cctacgccct cacgtggatc cgtcggctgg tggaagacga tgccaccgcc gtcgaagtgc
     1381 gcagggacgc ccaggccgcg tccaacgccc ggctgcagcg ccacctggag cggaccgtgt
     1441 gggcagcctc gtgccactcc tggtacaaga cggcatccgg caagatcacc aacaactggt
     1501 cgggtccgac cgtcgcctac tggtggcgga cccgacggcc ggaccgagcg gagttcgcca
     1561 cgacacccgg tcccggccgg cgccccgccc attcgggggc agcggggtcc tccgaatagg
     1621 gtcgacgtgc tgctcggcgc caacgttcgg cgccgagcag caaccgagca ccgcgcccat
     1681 cgaggagacc tcccaatgac caccgagccc gtacgcgtcg cagtcaccgg cggagccggc
     1741 cagatcggct acagcctgct gttccgcatc gccagcgggg ccctgctcgg cccggacacg
     1801 ccggtcatcc tgcagctgct cgagatcact ccggcgctcg gtgcgctcga cggcgtcgcc
     1861 atggagctcg aggactgtgc gttcccgctg ctcgccggcg tggtcaagac cgacgacgcc
     1921 gacgtggcct tcggcgacgt cgacatcgcc ctgctggtcg gctcccggcc ccggaccgcc
     1981 ggcatggagc gcggcgacct gctc
//
LOCUS       DATMFS010000271         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_79572, whole genome shotgun sequence.
ACCESSION   DATMFS010000271 DATMFS010000000
VERSION     DATMFS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79572"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VNQ33_05015"
     CDS             <1..425
                     /locus_tag="VNQ33_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97497.1"
                     /translation="DLSGRRVDVARDLDAMTVVIGVLGLVGGALAIANTTLVAVFERL
                     AEIALRRSLGASRGAIAGQFLVESAITGVLGGAVGAAIALLLLVVACAVRGWPLVLPG
                     TWIVGGPVVGAGVGVIAGALPAWRASTIAPAEILRGTR"
     gene            422..964
                     /locus_tag="VNQ33_05020"
     CDS             422..964
                     /locus_tag="VNQ33_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97498.1"
                     /translation="MSSPDPTAGLRIDPCGPEDLGFLYALAATVTQRWYRLCRRGLPP
                     PHEFEEALWQGVTCQFVTRSPDGTAIAVSSLHDLDLRNGIGWIDVVGDDDLHIDAVGP
                     TTLHLLDHAFGNWPLRKLYATHLDLQPSPFSAAGVPFDVEARLREFAWHDGMYWDRVI
                     TAVRRTQWTEHQAASQAGRR"
     gene            961..1557
                     /locus_tag="VNQ33_05025"
     CDS             961..1557
                     /locus_tag="VNQ33_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97499.1"
                     /translation="MSILSGARTVVRPLSPEHYETLRRAELTPMLGTVRFGGATPSPA
                     DHVRSLWTGVLLQSTAHDPASDELQALLTCYDADLRNGHAWIAIQAGPTAIGNGIAFA
                     GLALFVTDLFAEWRFRHLYAETSTSSFERFRSGEGQLFDVEGTLRDHHLRGSATSDKH
                     LLRIRRDRWMESIGAIVARRRAALERQPRALPRPDHGT"
     gene            1559..>2380
                     /locus_tag="VNQ33_05030"
     CDS             1559..>2380
                     /locus_tag="VNQ33_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97500.1"
                     /translation="MLLLDHIIASAVGGCRVWFPDAGEAAPIPLGELIEEAVALAPWI
                     EAEASGGAVGIPMTNDRGCLRAVLGAWLAGVPVASLPVPGRGGSAERYLEGLRAAIAT
                     SAVSIVIADTPVARLLGAHGVDATAFDEVPVRPGEPLRAVPVTFVQHTSGTTAAPRGL
                     RFDDGALLGNVEAVLRRLEVGPEAGSCSWLPLSHDMGFVGMTLASLVGAHRRADGVVT
                     IADPRRFVTDPAHWLRLCEATGSTITAAPNFALDLVVRTDRRRRIEGDLSSLRSCI"
BASE COUNT          304 a          898 c          818 g          360 t
ORIGIN      
        1 cggacctgtc gggccggcgg gtggacgtgg cccgagatct cgacgccatg accgtggtga
       61 tcggtgtgct gggcctcgtc ggcggcgcac tggcgatcgc gaacaccacg ttggtcgcgg
      121 tcttcgagcg gctcgccgag atcgcgctgc gtcgatccct cggtgcatcc cgtggcgcca
      181 tcgccggtca gttcctcgtc gagagcgcca tcaccggtgt cctcggcggg gccgtgggag
      241 cggccatcgc cttgctcctg ctggtggtgg cgtgcgctgt gcgcggctgg cccctcgtgc
      301 ttcccggtac ctggatcgtg ggcgggccgg tggtgggcgc gggcgttggc gtgatcgcgg
      361 gagccctgcc ggcgtggcgg gcgagcacca tcgctcccgc cgagatcctg cgaggaaccc
      421 ggtgagctcc ccagatccca ccgccggact gcgcatcgac ccctgcgggc ccgaggatct
      481 gggattcctc tacgcgctcg ccgccacggt gacccagcgc tggtaccgcc tctgccgccg
      541 ggggctcccg cctccccacg agttcgaaga agccctctgg cagggcgtga cctgccagtt
      601 cgtgacccga tcgcccgacg gcaccgcgat cgccgtctcc tcgttgcacg acctcgacct
      661 gcgcaacggc atcgggtgga tcgatgtcgt gggcgacgac gacctccaca tcgacgccgt
      721 cggccccacc acgctccacc tccttgacca cgccttcggc aactggccgc tgcgcaagct
      781 ctacgccacc cacctggacc tccagccctc tccgttcagc gcggctgggg tgccgttcga
      841 cgtggaagca cgcctccggg agttcgcttg gcacgacggc atgtactggg accgggtgat
      901 caccgccgtg cggcgcacgc agtggaccga gcaccaggcc gcgagccagg cgggccggcg
      961 atgagcatcc tgtcgggcgc gcgcaccgtc gtgcgaccgc tgtcgcccga gcactacgag
     1021 acccttcggc gagccgagct caccccgatg ctcggcaccg tccgcttcgg cggcgcgacc
     1081 ccgagcccgg cggaccacgt gcgctcgctg tggaccggcg tcctcctcca gagtacggcg
     1141 cacgaccccg cctcagacga gctccaggca ctcctcacct gctacgacgc cgacctccgg
     1201 aacggccacg cgtggatcgc gatccaagcg ggcccgacgg cgatcggcaa cggcatcgcc
     1261 ttcgccggcc tggcgctctt cgtgaccgac ctgttcgcgg agtggcggtt ccgccacctc
     1321 tacgcggaga ccagcacgtc gtcgttcgag cgcttccgga gcggcgaggg ccagctgttc
     1381 gacgtcgagg gcacgctgcg ggaccaccac ctgcgggggt cggccacctc ggacaagcac
     1441 ctgctgcgga tccgacggga tcggtggatg gagagcatcg gagcgatcgt cgcccgccgg
     1501 cgcgccgcgc tcgaacggca accccgcgcc ctgccgcgcc cggaccacgg gacctgacgt
     1561 gctgctgctc gatcacatca tcgcctcagc cgtcggtggc tgccgggtgt ggttccccga
     1621 tgccggggag gcagcgccga tcccgctcgg cgagctcatc gaggaggcgg tcgcgctcgc
     1681 cccgtggatc gaggcggagg ccagcggagg ggccgtcggc atcccgatga ccaacgaccg
     1741 cggctgcctc cgcgcggtgc tgggtgcctg gttggcagga gttcccgtgg cctccctgcc
     1801 cgttccgggg cggggcgggt ccgccgaacg gtacctcgag ggcctacgcg ccgcgatcgc
     1861 gaccagcgcg gtctccatcg tgatcgccga cacgcccgtc gccaggctcc tcggcgccca
     1921 cggcgtggat gcgaccgcgt tcgacgaggt ccccgtgcga ccaggagaac cgctccgtgc
     1981 cgtgccggtg accttcgtgc agcacacctc gggcaccacg gctgccccga gaggtctgcg
     2041 cttcgacgac ggcgcgctcc tcggcaacgt cgaggccgtg ctgcgccgcc tcgaggtagg
     2101 ccctgaggca gggagctgca gctggctgcc gctctcgcac gacatggggt tcgtgggcat
     2161 gaccctcgca tcgctcgtgg gcgcccaccg tcgcgccgat ggtgtggtga ccatcgccga
     2221 tccgcgccga ttcgtgaccg acccggccca ctggctccgg ctgtgcgagg ccaccggcag
     2281 caccatcacc gcagcgccca acttcgccct cgacctggtc gtgcggaccg accgacgtcg
     2341 tcgcatcgag ggcgacctgt cctccctccg ctcgtgcatc
//
LOCUS       DATMFS010000272         3340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_33364, whole genome shotgun sequence.
ACCESSION   DATMFS010000272 DATMFS010000000
VERSION     DATMFS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33364"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNQ33_05035"
     CDS             complement(<1..197)
                     /locus_tag="VNQ33_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97501.1"
                     /translation="MNVVVIKGVLSRAPELRELPSGDRLAQFEVTIRDPDRPTASVPV
                     ALLDPPERTLALDRGAEVVVTG"
     gene            complement(357..1562)
                     /locus_tag="VNQ33_05040"
     CDS             complement(357..1562)
                     /locus_tag="VNQ33_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ97502.1"
                     /translation="MAPNDRSSSRRGTGGRKPSTGSRSGTAGTRSDTSNSSKGAKPSS
                     GARKGAPPRSGAAPRGASGASRSGGGRGQGGRPQPDREEARTAGPKQWGSLGRKGAGR
                     LTAEEFDARDSVGARGRDERPAPAQRGSDPWVRVDDVRDEAASAVSRGKAKPAAKGPA
                     SRSTGGSRSGRKPPAEGPTKERRGRDYRQAGAGTGGAEAKTELVKALGAARGNRANER
                     LREAAHAFERERYTEARSLLKPIAEQAPQAATVRELLGLTYYRLGQWKLAVQELEAFR
                     QMTGSAEQNPVLADAYRGLKKPRQVEELWDELREASPSADLVTEGRIVMAGSLADRNK
                     LSDAIALLEKSHRGTKSPQIHHLRQAYALADLYERAGDIARARELFRWVAEREPDFAD
                     AAERASSIG"
     gene            1650..3275
                     /locus_tag="VNQ33_05045"
     CDS             1650..3275
                     /locus_tag="VNQ33_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97503.1"
                     /translation="MSNAPAAGEAAAPASADRSPDQAGMVLVALILVAAVANLNLAVA
                     NVALPDIGRAFDASQSSLNLIAVGYSLGLAASVLYLGALGDRYGRKMMLVFGISLSVP
                     ACILAAVAPNEQVLIVARLVGGVSAGMAYPTTLALITALWSGPGRTKSIALWSALGGA
                     ISSLGPLVSGALLEHFFWGSVFLVTLPLAVVALLLALRFVPAHVNEATEPVDNLGGIF
                     SAVLVGGLVLAINFAPLPSKRELAVGLSAIAVAAGVGFVVRQKRVANPLYDLDIAARR
                     IFWVAACAGIIVFGSLMGAMFIGQQFLQNVLEYDTLSAGAAILPAALLMIVAAPRSAK
                     LVEARGARFTLLVGYAFCFLGFLTMFTLWTDHAHYAVVALAYAFLGIGVGLAGTPASR
                     SLTSSVPVSKAGMASGTADLQRDLGGAVMQSILGAVLTAGYAKAFSASIAASPNADKV
                     SSAVQAQLTKSFSSAEVTAKQYPQYADQIIDAGRQAFVDGQDKAYLAGLLAVLLGGLL
                     VWFLFPKPDEERDLLASYLASDQTAAAAADPAT"
BASE COUNT          383 a         1285 c         1142 g          530 t
ORIGIN      
        1 ccggtgacga ccacctcggc cccgcggtcg agcgccaacg tccgctccgg cggatcgagc
       61 agggcgaccg gcaccgaggc ggtcggccgg tccggatccc ggatcgtgac ctcgaactgg
      121 gccagccggt cgcccgacgg cagctcgcgc agctccggcg cgcgagacag cacccctttg
      181 atgacgacga cgttcatgga accctccatg ttcatgccgg ccgagccggc gatgggtggt
      241 ggaggacggt tcgcacaccg tcgagcacgg cgggccccga gatccggagc cgtggcgacc
      301 gcccgtcagc gaacgggcgg cgatgcaccg aggaccctac gccgggggtc tgacggtcaa
      361 ccgatggagc tggcccgctc ggcggcgtcg gcgaagtcag gctcgcgctc ggcaacccag
      421 cggaagagct cccgggcccg ggcgatgtcg cccgctcgct cgtacagatc ggcgagggcg
      481 taggcctggc gcaggtggtg gatctgcggg ctcttggtgc cgcggtggct cttctcgagc
      541 aaggcgatgg cgtccgacag cttgttgcgg tcggcgagcg aaccggccat gacgatccgg
      601 ccctcggtca cgaggtccgc cgacggcgat gcctcccgca gctcgtccca cagctcctcg
      661 acctggcggg gcttcttcag cccccggtac gcatcggcga gcaccgggtt ctgctcggcc
      721 gagccggtca tctgccggaa ggcctccagc tcctgcacgg ccagcttcca ctggcccagc
      781 cggtagtagg tcagcccgag cagctcgcgc acggtcgccg cctggggcgc ctgctccgcg
      841 atcggcttga gcaggctccg ggcttcggtg tagcgctcgc gctcgaaggc atgggcggcc
      901 tcgcgcagcc gctcgttggc gcggttgccg cgcgccgccc cgagggcctt gaccagttcg
      961 gtcttggcct cggcaccacc cgtcccggcg ccggcctggc ggtagtcgcg gccgcgccgc
     1021 tccttggtgg gaccctccgc cgggggcttg cggccgctgc ggctgccgcc cgtcgaccgg
     1081 gaagccggtc ccttcgcggc cggcttggcc ttgccccggc tcacggcgct cgccgcctcg
     1141 tctcgaacgt cgtcgacgcg cacccacgga tccgagccgc gctgggcggg tgcgggccgc
     1201 tcgtcgcgac cccgggcgcc gacgctgtcg cgagcatcga actcctcggc ggtcaggcga
     1261 ccggcaccct tgcgaccgag gctcccccac tgcttgggac ctgcggtgcg ggcctcctcg
     1321 cggtccggtt ggggccggcc gccctggccg cggccgccgc cggagcggct ggcgcccgac
     1381 gcaccccgcg gcgcggcgcc gctgcgaggc ggcgctccct tgcgggctcc agaggagggc
     1441 ttggcgccct tcgagctgtt ggacgtgtcg gagcgagtgc cagcggtacc ggagcgggac
     1501 ccggtgctgg gcttccttcc accagtgccg cggcgcgacg aagagcgatc gttcggggcc
     1561 acggcgtcac cctatcgggg cgggtggttc cgataggttt ccggccaccg ggtgagcgct
     1621 caggcacccg ccaggaggga agcacacccg tgtccaacgc cccagcagcc ggtgaggctg
     1681 ccgcccccgc gtcggcggac cggtctccag accaagccgg gatggtgctc gtcgcgctga
     1741 tcctggtcgc cgccgtcgcc aacttgaacc tggccgtggc caacgtcgcc ctgcccgaca
     1801 tcggcagggc gttcgacgcg tcccagtcct cgctgaacct gatcgccgtc ggctactcgc
     1861 tcggcctggc cgcctcggtc ctgtacctgg gcgccctggg cgaccgctac ggccgcaaga
     1921 tgatgctcgt cttcggcatc tcgctgtcgg tgccggcgtg catcctggcg gccgtcgcac
     1981 cgaacgagca ggtcctgatc gtcgcccgcc tcgtcggtgg cgtgtccgcc ggcatggcct
     2041 atccgaccac gctggcgctg atcacggccc tgtggtcagg tcccggccgc accaagtcga
     2101 tcgccctgtg gtccgccctg ggcggcgcca tctcgtcgct cggcccgctc gtctccggcg
     2161 ccttgctcga gcacttcttc tggggctcgg tcttcttggt gaccctgccg ctcgcggtgg
     2221 tcgccctgct cttggccctg cgcttcgtcc ccgcccacgt caacgaggcc accgagcccg
     2281 tcgacaacct gggcggcatc ttctccgcgg tcctcgtcgg cggcctggtc ctggccatca
     2341 acttcgctcc cctgcccagc aagcgggagc tcgccgtcgg gttgtccgcc atcgccgtgg
     2401 ctgcgggcgt cgggttcgtg gtgcgccaga agcgggtcgc caacccgctc tacgacctcg
     2461 acatcgccgc tcgccggatc ttctgggtgg cggcctgcgc cggcatcatc gtgttcggct
     2521 cgctgatggg cgcgatgttc atcggccagc agttcctcca gaacgtgctc gagtacgaca
     2581 cgttgagcgc gggggcggcg atcctcccgg ccgccctgct catgatcgtc gccgcgcccc
     2641 ggtcagcgaa gctcgtcgag gcccgcggcg cccgcttcac cctcctggtg gggtacgcgt
     2701 tctgcttcct cggcttcctc acgatgttca ccctctggac cgaccacgcc cactacgccg
     2761 tggtcgccct cgcctacgcg ttcctcggca tcggcgtcgg gctggccggc acccccgcct
     2821 cgcgctcgct caccagctcg gtcccggtgt cgaaggccgg gatggcatcg ggcaccgccg
     2881 acctgcagcg cgacctcggc ggcgcggtga tgcagtccat cttgggcgcc gtcctcaccg
     2941 cgggctacgc caaggccttc tcggcatcga tcgccgcgtc gccgaacgcc gacaaggtct
     3001 cctcggccgt ccaggcccag ctcaccaagt cgttctcgag cgcagaggtg acagccaagc
     3061 agtacccgca gtacgccgac cagatcatcg acgccgggcg ccaggccttc gtcgacggcc
     3121 aggacaaggc ctacctggcc ggcttgctgg ccgtcctgct cggtgggctg ctggtctggt
     3181 tcctcttccc caagcccgac gaggagcgcg acctgctcgc ctcctacctc gcctcggacc
     3241 agaccgccgc cgcggctgcg gaccccgcca cctgacggcc gccaccagag gacctcggcg
     3301 tgccgacggc cggctctcgc gcgctcgcgg cctcccccac
//
LOCUS       DATMFS010000273         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_170871, whole genome shotgun sequence.
ACCESSION   DATMFS010000273 DATMFS010000000
VERSION     DATMFS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170871"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VNQ33_05050"
     CDS             <1..506
                     /locus_tag="VNQ33_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97504.1"
                     /translation="LTRRRWYLVAGLIAALAWIAGTAFAASAWDPLREAEVVPVGQPI
                     QANGSDDLAVLTDLPQAERAISCVARTGKKRTPVPPATVDIVVDSDGSRWHLIGVLTD
                     APDETRIVCRPDDKQTDTARYGTALVDLESRVGTGRAIAWTGLIAGLGLAGATLTART
                     RRILAHD"
     gene            499..1266
                     /locus_tag="VNQ33_05055"
     CDS             499..1266
                     /locus_tag="VNQ33_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWJ97505.1"
                     /translation="MTDLSLYVAAWRQSADAVLALEPADWDVPTDLPGWTAKDVLAHL
                     VHLERVMVEGETDAIGGGSVPADYTNAGVAALRDVPVEQLRADLADLVARRAKTLEEL
                     PDPQAAAVNTPAGVEWTWDVALRNRVIDLWSHEQDIRRAVGLPGGLDALGAQVTVGTF
                     AAALPYVLGRKAGAPAGSVVRWDVTGPVPVDSTIGVGDDGRARRTDAEPTATVRMDTE
                     AFVILGAGRRGPDGVEVEIDGDRELAERVLGAMAVTT"
     gene            1392..2051
                     /locus_tag="VNQ33_05060"
     CDS             1392..2051
                     /locus_tag="VNQ33_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97506.1"
                     /translation="MARRLRERTEQLTLAECRALDPTTHPRDFRQPNDTSCGATSLVV
                     SRMLHDEAYALWLTTGHDVRSGASDTAPFAERWPAEVVRMHRRVSGLRDHDGDLQWPW
                     LRVVGTSPWGAARQMTGAGGSGMPGHDYEARTLDPDDLGSAFDLVLAAVEAGHTVPLY
                     VGDEVRPAHVVLAVSARDGAVRFFEPSAGRLVRVDREAFATATLRLGGWSVPWFAVLP
                     R"
BASE COUNT          265 a          830 c          752 g          267 t
ORIGIN      
        1 agctgacccg aagacgctgg tacctggtgg cgggcctgat cgccgcgctg gcgtggatcg
       61 ccggcaccgc gttcgcggcc tccgcatggg atccgctgcg cgaggccgag gtcgtcccgg
      121 tgggacagcc catccaggcg aacgggtcgg acgacctcgc cgtcctgacc gacctgccgc
      181 aggccgagcg cgcgatctcg tgcgtcgccc gcacgggcaa gaagcgcacc ccggtgccgc
      241 cggccacggt ggacatcgtg gtcgacagcg acggcagccg ctggcacctc atcggcgtcc
      301 tgaccgacgc ccccgacgag acgcgcatcg tgtgccgtcc cgacgacaag cagaccgaca
      361 ccgctcgcta cgggaccgcc ctggtggacc tcgagagccg cgtcgggacg ggccgggcga
      421 tcgcctggac cgggctcatc gcgggactgg ggctggccgg tgccaccctg accgcacgca
      481 cccgcaggat cctcgcccat gactgacctc agcctgtacg tcgccgcctg gcggcagtcc
      541 gccgacgccg tgctcgccct cgagcccgcc gactgggacg tccccaccga cctgcccggg
      601 tggaccgcga aggacgtcct cgcgcatctg gtccacctcg aacgcgtgat ggtcgagggc
      661 gagaccgacg cgatcggcgg cgggagcgtg ccggccgact acacgaacgc cggcgtcgcc
      721 gccctgcgcg acgtcccggt ggagcagttg cgcgccgacc tggccgacct cgtcgcccga
      781 cgtgctaaga ccctcgagga gctccccgac ccgcaggccg cagccgtgaa caccccggcc
      841 ggtgtggagt ggacgtggga cgtcgcgctg cgcaaccgcg tgatcgacct ctggtcgcac
      901 gagcaggaca tccgccgcgc cgtcgggctg cccggtggac tcgacgcgct tggcgcgcag
      961 gtcaccgtgg gcacgttcgc cgcggcgctg ccctacgtgc tgggccgcaa ggccggggcg
     1021 cccgccggca gcgtcgtgcg ctgggacgtc accggcccgg tccccgtgga ctccacgatc
     1081 ggcgtgggcg acgacggtcg cgctcgccgc accgacgccg agcccaccgc gaccgtgcgg
     1141 atggacaccg aggcgttcgt gatcctcggg gccggtcgcc gcggccccga cggggtcgag
     1201 gtcgagatcg acggcgaccg ggagctggcc gagcgcgtcc tgggcgccat ggccgtcacc
     1261 acctgatgcg tcccgggccg gcagtccccg tcgcgcggct ggtccgcctg gcacgacgcg
     1321 gcctcgcccg actccagcgc accccgcgca ctccgcgggg attcaccgca ccccaggccc
     1381 tgccgcccga ggtggcgcga cgcctgcgcg agcgcaccga gcagctgacc ctcgccgagt
     1441 gccgtgccct cgacccgacg acccacccgc gcgacttccg ccagcccaat gacacctcgt
     1501 gcggcgcgac ctcgctcgtc gtgtcgcgga tgctgcacga cgaggcgtac gcgctgtggt
     1561 tgaccaccgg ccacgacgtg cgcagcggcg ccagcgacac cgcgccgttc gccgagcgct
     1621 ggcccgcgga ggtcgtgcgg atgcaccgcc gcgtctctgg cctgcgcgac cacgacggcg
     1681 acctccagtg gccctggctg cgcgtcgtcg gcacctcacc ctggggcgcc gcccgacaga
     1741 tgaccggcgc cggcggctcc gggatgcccg gtcacgacta cgaggcacgc actctcgacc
     1801 ccgacgacct cggctccgcc ttcgacctcg tcctggcggc cgtcgaggcc ggccacaccg
     1861 tgccgctcta cgtgggcgac gaggtccgtc cggcgcacgt cgtgctggcc gtgtccgccc
     1921 gagacggggc agtgcgcttc ttcgagccga gcgccggccg cctcgtgcgg gtcgaccgcg
     1981 aggcgttcgc gaccgccacc ctgcggctcg gcggctggtc cgtgccgtgg ttcgcggtgc
     2041 tgccacgctg atccggacat ggcgactggg cggccacacg cttcgctcga gaggtcggtc
     2101 cgggcacctt cggg
//
LOCUS       DATMFS010000274         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_289534, whole genome shotgun sequence.
ACCESSION   DATMFS010000274 DATMFS010000000
VERSION     DATMFS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289534"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VNQ33_05065"
     CDS             complement(<1..1011)
                     /locus_tag="VNQ33_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97507.1"
                     /translation="MDFELPADDDPRRVAVRAWLVEHPSPSGADLAAAGYVSPHWPEP
                     WGLAADPIHQLIIDDELRAAGVRRPNNPIGIGWAGPTLIHAGTPEQQARYLPKLLSGE
                     EIWCQLFSEPEAGSDLANLATRAVRDGDEWIVNGSKIWTSLAQFATYGILIARTDPDV
                     SKHQGISYFVCPMDLPGIEIRPIIEMTGGHTFNEVFLTDVRLPADSLVGDVNNGWSLA
                     KVTLGNERVSLSSGGALWGMGPNGADLLDLVRAAGGVADPLVRQRLARLHTTEEILRL
                     IRLRTVSALVRGSAPGPEASIRKVMADEHGQAIMELAKDLAGPGGLLSDTGPLGTPPE
                     VWN"
     gene            1107..>2325
                     /locus_tag="VNQ33_05070"
     CDS             1107..>2325
                     /locus_tag="VNQ33_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97508.1"
                     /translation="MAGWNYAEIWEVIAAQIPEAPAQIQGGRTLTWREFDQRANGVAH
                     ALLEAGAQEQDKVAQYLYNCPEYLESMFAAWKAGLVPVNTNYRYADDELVYLWDNADA
                     VAVVFHGSFTGTIERILDRVPDVKTWLWVDDGSGPCPDWATPYEDAAASHPEKVEAPW
                     GRDGDHLYMLYTGGTTGMPKGVMWRQDDHIRNIIALGTNPRYAEDPVDYGVPAELVGG
                     PGIVSLPACPLMHGTGCMTQLIALSGGGCVVTLEGRNLDIEELFDTIERHKANTTAIV
                     GDAFAKPMLRALDANPGRWDLSSMFVFTSSGVMFSEPSKQRLIEHLPHAMIVDAFSSS
                     EAVGMGQSVSAAGMDPDTAKFAQGPNTKVFTEDLREVAPGSGEIGRVAVGGHQPIGYY
                     KDPEKTAATFIEVD"
BASE COUNT          360 a          817 c          827 g          321 t
ORIGIN      
        1 gttccagacc tcgggcggcg tgcccagggg gcccgtgtcg ctgagcaggc ccccggggcc
       61 ggcgaggtcc ttcgccagct ccatgatcgc ctggccgtgc tcgtcggcca tgaccttgcg
      121 gatcgaggcc tcgggaccgg gcgccgagcc ccgcaccagg gcggagacgg tgcgcaggcg
      181 gatgaggcgc aggatctcct cggtggtgtg caggcgggcg agccgctggc gtacgagcgg
      241 atcggcgacc ccgccggcgg cccggacgag atcgagcagg tcggcgccgt tcggtcccat
      301 cccccacagc gcgccgcccg acgagagcga gacccgttcg ttgccgaggg tgaccttggc
      361 gagggaccac ccgttgttca cgtcgcccac gagggagtcg gccgggagcc ggacgtcggt
      421 gaggaacacc tcgttgaagg tgtgaccgcc ggtcatctcg atgatgggcc ggatctcgat
      481 gccaggcagg tccatcgggc acacgaagta cgagatcccc tggtgcttgg agacgtcggg
      541 gtcggtgcgg gcgatgagga tgccgtaggt ggcgaactga gcgagcgagg tccagatctt
      601 ggacccgttg acgatccact cgtcgccgtc gcgcacggcg cgggtggcga ggttggccag
      661 gtcgctgccg gcctcgggct cgctgaagag ctggcaccag atctcctcgc ccgacagcag
      721 cttgggcagg taccgggcct gctgctcggg cgtgccggcg tggatgaggg tcggcccggc
      781 ccagccgatg ccgatggggt tgttcggccg gcggacgccc gccgctcgga gctcgtcgtc
      841 gatgatcagc tggtggatcg gatcggcggc cagcccccac ggctcgggcc agtgcggcga
      901 gacgtacccg gcggccgcca ggtccgctcc gctgggcgag gggtgctcga ccagccaggc
      961 gcgcaccgcc acgcgtcgag ggtcgtcgtc ggccggcagc tcgaagtcca cgaggggtga
     1021 ccgtacccaa gcgcgagacc ggctggtcgc ccgccacagc ggtggtccta tgctcggctc
     1081 tcgaccgaca cagcacaggg gacgacatgg ctggatggaa ctacgcagag atctgggagg
     1141 tcatcgccgc ccagatcccg gaggcaccgg cgcagatcca gggcggccgc acgctcacgt
     1201 ggcgggagtt cgaccagcgg gccaacggcg tcgcccacgc cctcctcgag gcgggcgccc
     1261 aggagcagga caaggtcgcg cagtacctct acaactgccc cgagtacctc gagtcgatgt
     1321 tcgcggcgtg gaaggccggc ctcgttccgg tgaacacgaa ctaccggtac gccgacgacg
     1381 agctggtcta cctgtgggac aacgccgacg ccgtggccgt ggtgttccac gggtccttca
     1441 ccggcaccat cgagcgcatc ctcgaccgcg tgcccgacgt gaagacctgg ctgtgggtcg
     1501 atgacggcag cggcccctgc ccggactggg cgaccccgta cgaggacgcg gccgcgtcgc
     1561 accccgagaa ggtcgaagcc ccctggggcc gcgatggcga ccacctctac atgctctaca
     1621 cgggcggcac cacgggcatg cccaagggcg tcatgtggcg ccaggacgac cacatccgca
     1681 acatcatcgc cctgggcacc aacccccgct acgccgagga ccccgtcgac tacggcgtgc
     1741 ccgccgagct ggtgggcgga cccggcatcg tcagcctccc ggcctgcccc ctgatgcacg
     1801 gcaccggctg catgacgcag ctgatcgccc tctccggcgg cggctgcgtc gtcacgctcg
     1861 agggccgcaa cctcgacatc gaggagctgt tcgacacgat cgagcgccac aaggccaaca
     1921 ccacggccat cgtcggcgac gccttcgcca agccgatgct gcgggcgctc gacgccaacc
     1981 ccggccgctg ggacctgtcc agcatgttcg tgttcacgtc gtcgggcgtc atgttctcgg
     2041 agccgtcgaa gcagcggctc atcgagcacc tcccccacgc catgatcgtc gacgcgttca
     2101 gctcgtcgga ggccgtcggc atgggccagt cggtgtcggc cgccggcatg gaccccgaca
     2161 cggcgaagtt cgcccagggc cccaacacca aggtgttcac cgaggacctc cgcgaggtcg
     2221 cgcccgggtc gggtgagatc gggcgggtcg ccgtcggcgg ccaccagccc atcgggtact
     2281 acaaggaccc cgagaagacg gcggccacct tcatcgaggt cgacg
//
LOCUS       DATMFS010000275         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_149898, whole genome shotgun sequence.
ACCESSION   DATMFS010000275 DATMFS010000000
VERSION     DATMFS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149898"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /gene="dinB"
                     /locus_tag="VNQ33_05075"
     CDS             <1..1251
                     /gene="dinB"
                     /locus_tag="VNQ33_05075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWJ97509.1"
                     /translation="AFFVSVELLRRPELRGKPVVVGGSGARGVVAAASYEARSYGVHS
                     AMPGARARRLCPHAVFLPGDHALYSEVSGRVMDVFRSFTPLVEPISLDEAFLDVRGAR
                     RLMGTPVEIAHRLRARVLDQEGLTCSVGIARVKFLAKLASEEAKPKASPSGPVFGDGV
                     FEVLPDRELAFLHALPAQALWGVGPATLARLERLGVRTVGDLAALPLGTVTGALGRAS
                     GTHLHALANARDPRPVVPEQQARSVSHEETFSRDRFRREELEVEAVRLADAVATRLRR
                     GGLTGRTVTVKVRFGDFSTITRSATVDGGVDSGHELAQIARRLLSEVDISPGVRLLGV
                     GVSQLGDQAGRGGRQLSLDDLLAGADAVSRADWTSAEEAMDAVRARFGDTALGPASLA
                     GPGGLRVARKGQQQWGPDEDPQKG"
     gene            1354..1776
                     /locus_tag="VNQ33_05080"
     CDS             1354..1776
                     /locus_tag="VNQ33_05080"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HWJ97510.1"
                     /translation="MPLSEDEQRILQQIEQQFYATDPDLAGEIGNHSLYTHCLRRMRW
                     AAAVFAVGLVILVVALFFGASFLISFGGFVVMLGAALWFERSLRKMGRAGMAQLSASL
                     HTGGLRDYFGETGDRVRNRLRPHDDGGDAEDEADTDRS"
     gene            complement(1802..>2058)
                     /locus_tag="VNQ33_05085"
     CDS             complement(1802..>2058)
                     /locus_tag="VNQ33_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWJ97511.1"
                     /translation="LQVSRRLATRAGVPLDPAETPFELVERARPSVGGAVAADLEVLA
                     TLVTEARWSPGGLSEPAQARLDALAERVVAAIDHPAPAPA"
BASE COUNT          242 a          768 c          762 g          286 t
ORIGIN      
        1 gcgttcttcg tgtccgtcga gctcctccgc cgaccggagc tgcggggcaa gcccgtcgtg
       61 gtcggcggca gcggcgcccg cggcgtggtg gcggcggcgt cctacgaggc ccgctcgtac
      121 ggcgtccact cggcgatgcc cggcgcccgg gcccggcgcc tgtgcccgca cgccgtgttc
      181 ctccccggcg atcacgccct ctacagcgag gtgagcgggc gggtgatgga cgtgttccgg
      241 tccttcaccc cgctggtgga gccgatctcg ctcgatgagg cgttcctcga cgtgcggggc
      301 gcccggcgcc tgatgggcac gcccgtcgag atcgcccacc ggctgcgggc ccgggtcctc
      361 gaccaggagg gcctcacctg ctcggtgggc atcgcccggg tgaagttcct ggccaagctg
      421 gcgtccgagg aggccaagcc gaaggcatcg ccgagcggcc cggtcttcgg cgacggcgtg
      481 ttcgaggtgc ttccggatcg ggagctggcc ttcctccacg ccctgccggc ccaggcgctg
      541 tggggcgtcg gccccgccac cctcgcccgc ctcgagcggc tgggcgtgcg cacggtgggc
      601 gacctggcgg ccctgcccct cggcaccgtc acgggcgcgc tcggccgggc ctcgggcacc
      661 cacctccacg ccctcgccaa cgcccgggac cctcgtccgg tggtgcccga gcagcaggcg
      721 aggtcggtca gccacgagga gaccttcagc cgggaccgct tccgccggga ggagctggag
      781 gtcgaggccg tccgcctggc cgacgccgtc gccacccgcc tgcgccgcgg cggcctcacc
      841 ggccgcaccg tcacggtcaa ggtccgcttc ggcgacttca gcaccatcac ccgctcggcc
      901 accgtcgacg gcggggtgga ctccggtcac gagctggccc agatcgcccg ccgcctcctg
      961 agcgaggtcg acatctcgcc cggtgtccgg ctgctcggcg tgggggtctc gcagctcggc
     1021 gaccaggccg gtcggggcgg gcgccagctc tcgctcgacg acctgctggc aggagcggac
     1081 gcggtgtccc gggccgactg gacgtcggcc gaggaggcca tggacgccgt ccgggcccgt
     1141 ttcggcgaca ccgcgctggg cccggcgagc ctcgccggac cgggtggcct gcgcgtcgcc
     1201 cgcaagggcc agcagcagtg gggacccgac gaggaccccc agaagggctg aggatccggg
     1261 gcgggaacag gtccgaacgc gttgtcgcgt gcctcccgct gtgcgacact gaacggaccg
     1321 ggtcggggcc cggcggtcga aaggttgacg gacgtgccgc tatctgagga cgagcagagg
     1381 atcctccagc agatcgagca gcagttctac gcgaccgacc ccgatctggc gggcgagatc
     1441 ggcaaccact cgctctacac ccactgcctg cgccggatgc gctgggctgc ggccgtgttc
     1501 gcggtcggcc tcgtgatctt ggtcgtcgcc ctgttcttcg gcgccagctt cttgatctcg
     1561 ttcggcggct tcgtggtgat gctcggcgcc gccctctggt tcgagcgcag cctgcgcaag
     1621 atgggccggg ccggcatggc ccagctctcg gcctcgctcc acaccggcgg cctccgcgac
     1681 tacttcggcg aaaccggcga ccgcgtccgc aaccgcctcc gtccccacga cgacggcggc
     1741 gacgccgaag acgaagccga caccgaccgc tcctgaaccg ctcgaacgcc ggtgctgcgg
     1801 cctaggccgg cgccggggcc gggtggtcga tcgcggccac cacgcgctcg gctagggcgt
     1861 cgaggcgggc ttgggctggc tcggacaggc cgccggggga ccacctggct tcggtgacca
     1921 gcgtggcgag cacctcgagg tcggcggcga cggcgccacc gacgctcggc ctggcccgtt
     1981 cgacgagctc gaagggggtc tcggcggggt cgaggggcac gccggctcgg gtggccaggc
     2041 gcctgctcac ctggagcc
//
LOCUS       DATMFS010000276         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_33547, whole genome shotgun sequence.
ACCESSION   DATMFS010000276 DATMFS010000000
VERSION     DATMFS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33547"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VNQ33_05090"
     CDS             complement(<1..515)
                     /locus_tag="VNQ33_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HWJ97512.1"
                     /translation="MPVGAGGHEQALAARGVRKELGAYYTPPDVVDGLLDLVLDPILA
                     ERSSGGADAVSAVRVVDPACGTGNFLVAVAARVGAALERLGLEPGEARRRAVRCVRGV
                     ELDAATARLCRDALKAVHVSGGGRSVVRGDALLDDLLPAGRFDLVVGNPPFLSQLASS
                     TARTRGDAEALR"
     gene            583..1473
                     /locus_tag="VNQ33_05095"
     CDS             583..1473
                     /locus_tag="VNQ33_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97513.1"
                     /translation="MSVHLRTPRAVVALVLGLTLALAACGGGSDDTASSDGGKGTAAT
                     APDTDTGSIPALDGGTHQFDAVDGSKARFINLLAKDGEGVGIDVYWGANAETGKKAAT
                     VAYGEVSDWETFQVDQDSFLTRTDGKEEVVVTFYPEGETERPIIQQAEPIEGDMRLTY
                     TMGTGDGIGLSADQYPGSLGVGFDHEAGDPPAGKAWVAFNGSGLGGIDDGDFMVLSTA
                     DGCNDLEGTDIEGGTANSGQAYLVDVGTQSFTASDANTECAERLDPVDIDFAEGDQYV
                     VYAYGTSLDDRRLMAVKVGE"
     gene            1601..>3373
                     /locus_tag="VNQ33_05100"
     CDS             1601..>3373
                     /locus_tag="VNQ33_05100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ97514.1"
                     /translation="MTDLTDDASASTPEPAATPEPATTLDAAAEPEAAEPEAPASAPE
                     PAAELGAEPGLAAPEDDGPTFADLGLRVEILDALNALGYEEPTPIQRETIPTIIAGRD
                     LLGQAATGTGKTAAFALPILERLVPDAAVEPIALVLVPTRELAVQVSEAIYKYGRELD
                     AQVLPIYGGQPIIHQLKVIKRGVHVVVATPGRAVDHINRGTLPLDAVELVVLDEADEM
                     LDMGFAEDIDAILDACPFERQTVLFSATMPSRIASIAKRHLKDPVRIQVNREAVAAGE
                     SPRVRQSAYLVARSHKPAALGRILDVESPTAAIVFCKTRGEVDELTETLNGRGYRAEA
                     LHGGMNQEQRDRVMARLRSGTAELLVATDVAARGLDVDQLTHVVNYDVPSAPESYVHR
                     IGRVGRAGREGVAITLAEPRQRRLLDNIERLTKQTIKIEKIPTVADLRARQMELTLDQ
                     VREALESGDDRNRFRAVVDALADEFDMADVALAAVKLVHEASGATEDLEEIPDIATRD
                     KPKHKGDRWDKGTKGKHKDKPWEKDKPGKGKGAKSWDKGDRGPQGPTSRLYVGAGRGD
                     GVRPQDLVGAIAGETHLRGNDIGAI"
BASE COUNT          562 a         1297 c         1145 g          369 t
ORIGIN      
        1 cgaagcgcct cggcatcacc ccgggtccgg gcggtcgacg aggccagctg gctgaggaac
       61 ggcgggtttc ccaccaccag gtcgaaccgg ccggcgggga ggaggtcgtc cagcagcgca
      121 tcgccccgca ccaccgaccg cccgccaccg gagacgtgga ccgccttgag cgcgtcccgg
      181 cacaggcggg cggtcgccgc gtcgagctcc acgccccgga cgcagcgcac ggcccggcgg
      241 cgcgcctcgc cgggctcgag acccagccgc tcgagcgccg cgccgacgcg cgccgccacc
      301 gccacgagga agttgccggt gccgcaggcc ggatcgacca cccgtaccgc ggagaccgca
      361 tccgcacccc cgctggaccg ctcggcgagg atcgggtcga gtacgaggtc gagcagcccg
      421 tcgaccacat cgggcggcgt gtagtacgca ccgagctcct tgcggacccc gcgggcggcg
      481 agcgcctgct cgtgacctcc cgcacccacc ggcatcgagc cagcctggca tctgcgagcg
      541 gggtagcgtc ccggacgaga cgaacgacct gcgggaggtc ccatgtctgt tcacctgcgc
      601 acaccgcgcg ccgtggtcgc cctggtcctc gggctcacgc tcgccctggc cgcgtgcggc
      661 ggcggctccg acgacaccgc gtcgtccgac ggcggcaagg gcaccgccgc cacggcaccg
      721 gacaccgaca ccggctcgat cccggccctc gacggcggaa cccaccagtt cgacgccgtc
      781 gacggctcga aggcccggtt catcaacctg ctcgcgaagg acggcgaggg cgtcggcatc
      841 gacgtctact ggggcgccaa cgcggagacc ggcaagaagg cggcgaccgt cgcctacggc
      901 gaggtgagcg actgggagac gttccaggtc gaccaggact cgttcctcac gcgaaccgac
      961 gggaaggagg aggtcgtcgt gaccttctac cccgagggcg agaccgagcg gccgatcatc
     1021 cagcaggccg agcccatcga gggcgacatg cggctgacct acacgatggg cacgggcgac
     1081 ggcatcggcc tcagcgcgga ccagtacccg ggctcgctcg gcgtcggctt cgaccacgag
     1141 gcgggggatc cgcccgccgg caaggcatgg gtggccttca acggcagcgg gctcggcggc
     1201 atcgacgacg gtgacttcat ggtcctgagc accgcggacg gctgcaacga cctcgaaggc
     1261 accgacatcg agggcggcac ggccaactcc ggccaggcct acctcgtcga cgtcggcacc
     1321 cagtcgttca ccgcatcgga cgccaacacg gagtgcgccg agcggctcga ccccgtcgac
     1381 atcgacttcg ccgagggcga ccagtacgtc gtctacgcct acggcacgag cctcgacgac
     1441 cggcggctga tggcggtcaa ggtcggcgag tagcccacag gctggggatc tggcgcggcc
     1501 agggcgtagc ctggacggtc caccgttcga gtcacaccga cgaccaccgc ccgcacgatc
     1561 gggccacgca cgtcgaatcg gctcaccact gggagccacc atgaccgatc tgaccgacga
     1621 cgcgtccgcg tcgacccccg aaccggctgc gacgcccgag ccggccacga ccctcgatgc
     1681 ggccgccgaa cccgaagctg cggagccgga ggcgccggcc tccgcgcccg agcccgctgc
     1741 tgagctcggc gccgagcccg ggctggccgc acccgaagac gacggcccca cgttcgccga
     1801 cctcggcctg cgggtcgaga tcctcgacgc cctcaacgcc ctcggctacg aggagcccac
     1861 gccgatccag cgcgagacga tcccgacgat catcgccggc agggacctgc tcggccaggc
     1921 ggcgacgggc accggcaaga ccgcggcgtt cgcgctgccg atcctcgagc gcctcgtccc
     1981 cgacgccgcc gtcgagccga tcgccctggt cctcgtaccg acccgggagc tggcggtgca
     2041 ggtgtccgaa gcgatctaca agtacggccg cgagctcgat gcccaggtgc tgcccatcta
     2101 cggcggccag ccgatcatcc accagctcaa ggtcatcaag cgcggggtcc acgtggtggt
     2161 cgccacgccc ggccgcgccg tcgaccacat caaccggggc acgctcccgc tcgatgccgt
     2221 cgagctggtg gtgctcgacg aagccgacga gatgctcgac atgggcttcg ccgaagacat
     2281 cgacgccatc ctcgacgcct gcccgttcga gcggcagacc gtgctgttct ccgccacgat
     2341 gccgtcgcgc atcgcgtcca tcgccaagcg ccacctcaag gacccggtcc gcatccaggt
     2401 gaaccgcgag gccgtcgccg ccggcgagtc gccccgcgtc cgccagagcg cctacctcgt
     2461 ggcccgcagc cacaagcccg ccgcgctcgg gcgcatcctc gacgtggagt cgccgaccgc
     2521 agcgatcgtg ttctgcaaga cccgaggcga ggtcgacgag ctcaccgaga cgctcaacgg
     2581 ccgcggctac cgagccgaag ccctccacgg cggcatgaac caggagcagc gcgaccgggt
     2641 catggcccgc ctccgctccg gcaccgccga gctgctcgtc gccaccgacg tcgccgcccg
     2701 cggcctcgac gtcgaccagc tgacccacgt cgtcaactac gacgtgccca gcgcgccgga
     2761 gtcctacgtg caccgcatcg ggcgcgtggg ccgggccggg cgcgagggcg tggccatcac
     2821 cctggccgag ccccgccagc gccggctgct cgacaacatc gagcggctca cgaagcagac
     2881 catcaagatc gagaagatcc ccaccgtggc cgacctccgc gcccggcaga tggagctcac
     2941 cctcgaccag gtgcgcgagg cgctggagtc cggcgacgac cggaaccgct tccgggccgt
     3001 ggtcgacgcc ctcgccgacg agttcgacat ggccgacgtg gccctcgccg ccgtgaagct
     3061 ggtccacgag gccagcggcg ccaccgagga cctcgaggag atccccgaca tcgcgacgcg
     3121 cgacaagccc aagcacaagg gcgacaggtg ggacaagggc accaagggca agcacaagga
     3181 caagccctgg gagaaggaca agcccggcaa gggcaagggc gccaagtcct gggacaaggg
     3241 cgaccgcggc ccccagggcc cgacctcgcg gctctacgtg ggcgccggcc ggggcgacgg
     3301 cgtacggccc caggacctcg tgggcgccat cgccggcgag acgcacctgc ggggcaacga
     3361 catcggcgcc atc
//
LOCUS       DATMFS010000277         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_33565, whole genome shotgun sequence.
ACCESSION   DATMFS010000277 DATMFS010000000
VERSION     DATMFS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33565"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VNQ33_05105"
     CDS             complement(<1..399)
                     /locus_tag="VNQ33_05105"
                     /inference="COORDINATES: protein motif:HMM:NF045164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97515.1"
                     /translation="MSQFDSQTSTIDGHSYTVRMLPARRATRMLAKLSSMIGPALGTL
                     AEGGKLSDLADAKVNGKLFSRAAVALFAHVDEDAVDAILMELADVTTVEPGGLLKPIY
                     DVHFMGRQGALMKWAAFALRVQYADFFDVAS"
     gene            complement(399..818)
                     /locus_tag="VNQ33_05110"
     CDS             complement(399..818)
                     /locus_tag="VNQ33_05110"
                     /inference="COORDINATES: protein motif:HMM:NF023110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HWJ97516.1"
                     /translation="MAVETYAPEELVITFGPVLMSGFADADVTIEHNEDSFTFKAGLD
                     GKGTRTKNANKSGRVTVPLMQTSLANDLLSAIAQVDRKTGQGVFPLLAKDLSGRTVYA
                     AQDAWIVKPPASGFGKEAGQRDWIFETDNLEFHVGGN"
     gene            complement(831..>2054)
                     /locus_tag="VNQ33_05115"
     CDS             complement(831..>2054)
                     /locus_tag="VNQ33_05115"
                     /inference="COORDINATES: protein motif:HMM:NF023291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HWJ97517.1"
                     /translation="FPERFRVYTAVADMVEDGFPASSAIVRAVSAILSQQPKVQRVIV
                     GRRDGKPTTKHALTPTAVHLAKYTVTVDGKEASFTADATSTVAKVSAGLVAAINAVSG
                     GAVTAAAVGGAGTETGLTITAKVAGEVYSLELATTLGAKLSVEETTADVGIADDIAAL
                     ALATGDWYGLVLVSQGKAEILAAAAVIESMRRILGVTSMDSDILDGVATTDLASQLKA
                     AGYARTFLLYSPAAHEYGAAAWMGNRFPGQPGGSTWKFKTLRGVSVYSLTASQEAAAK
                     AKNANTYTSIGGVSITAEGVTASGEFIDVTHGVDWLQAQLQEGVYSLLVNSDKVPFTD
                     GGVALVENVVRGCLARGVSADLLAADPEPTVSVPRVRDVPQPDRAARHLPDVRFEAVL
                     AGAVHSLTISGTITV"
BASE COUNT          299 a          693 c          744 g          318 t
ORIGIN      
        1 gctcgccacg tcgaaaaaat cggcgtactg cacgcgcagc gcgaaggccg cccacttcat
       61 cagcgcgccc tgtcggccca tgaagtgcac gtcgtagatg ggcttgagca gcccgcccgg
      121 ctccacggtg gtgacgtcgg ccagctccat caggatcgcg tccaccgcgt cctcgtcgac
      181 gtgcgcgaag agcgccaccg cggcgcgcga gaagagcttg ccgttcacct tcgcgtcggc
      241 gaggtcagag agcttgccgc cctcggccag cgtgccgagt gccgggccga tcatgctgga
      301 gagcttcgcc agcatccgcg tcgcccggcg ggccgggagc atgcgaacgg tgtagctgtg
      361 cccgtcgatc gtcgacgtct gcgaatcgaa ctgggacatc agttgccccc aacgtggaac
      421 tcgaggttgt ccgtctcgaa gatccagtcg cgctgcccgg cctccttgcc gaagcccgac
      481 gccggaggct tcacgatcca ggcgtcctgc gcggcgtaga cggtgcggcc ggagaggtcc
      541 ttggcgagga gcgggaagac gccctgcccc gtcttgcgat cgacctgggc gattgccgag
      601 aggaggtcgt tcgcgaggct cgtctgcatg agcggcacgg tgacgcggcc cgacttgttc
      661 gcgttcttcg tccgcgtgcc cttgccgtcg agcccagcct tgaaggtgaa gctgtcctcg
      721 ttgtgctcga tcgtcacgtc ggcgtcggcg aagcccgaca tgagcaccgg gccgaaggtg
      781 atgacgagct cttcgggagc gtaggtttcg actgccatgg tggacctcga tcagaccgtg
      841 atggtcccgg agatggtgag cgagtggacg gcgccggcga ggacggcctc gaagcgcacg
      901 tcgggaaggt ggcgggcggc gcggtccggc tgcggcacgt cccgcacgcg ggggaccgag
      961 acggtgggct cggggtcggc ggcgaggaga tccgccgaca cccctcgggc taggcagccg
     1021 cgcaccacgt tctcgacgag ggccacgccc ccgtccgtga acggcacctt gtcgctgttc
     1081 acgaggaggg agtagacgcc ctcctggagc tgggcctgga gccagtccac gccgtgggtg
     1141 acgtcgatga actcgcccga ggcggtgacg ccctcggccg tgatcgagac gccgccgatc
     1201 gaggtgtacg tgttcgcgtt cttcgccttc gccgcggcct cctgggaggc ggtgagcgag
     1261 tagacggaga cgccccgcag cgtcttgaac ttccacgtgc tcccgcccgg ctgcccgggg
     1321 aagcggttcc ccatccacgc cgcggcgccg tactcgtgcg ccgcgggcga gtagaggagg
     1381 aaggtgcgcg cgtagcccgc ggccttgagc tgcgaggcca ggtcggtcgt cgccacgccg
     1441 tcgaggatgt cggagtccat ggacgtgacg cccaggatcc gacgcatcga ctcgatcacg
     1501 gcggcggcgg cgaggatctc cgccttgccc tgggagacga ggacgaggcc gtaccagtcg
     1561 ccggtcgcca gcgcgagggc cgcgatgtcg tcggcgatcc ccacgtcggc cgtggtctcc
     1621 tcgacggaga gcttcgcgcc cagcgtggtg gcgagctcga gggagtagac ctcgccggcc
     1681 accttcgcgg tgatggtgag cccggtctcg gtcccggcgc cgcccaccgc ggccgccgta
     1741 acggcgccgc ccgagacggc gttgatcgca gccacgagcc cggcggacac cttggcaacg
     1801 gtggacgtgg cgtcggccgt gaagctggcc tccttgccgt cgacggtcac ggtgtacttc
     1861 gcgaggtgca ccgcagtcgg ggtgagcgcg tgcttcgtcg tgggcttgcc gtcccggcgc
     1921 cccacgatca cccgctgcac cttcggctgc tgcgagagga tcgcggagac cgcacgcacg
     1981 atcgcgctgc tcgccgggaa gccgtcctca accatgtcgg ccaccgcggt gtagacgcgg
     2041 aagcgctcag ggaa
//
LOCUS       DATMFS010000278         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_312558, whole genome shotgun sequence.
ACCESSION   DATMFS010000278 DATMFS010000000
VERSION     DATMFS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312558"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /gene="aceE"
                     /locus_tag="VNQ33_05120"
     CDS             complement(<1..1882)
                     /gene="aceE"
                     /locus_tag="VNQ33_05120"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWJ97518.1"
                     /translation="MGIDDGFVGQLYDADPDETREWLDSLDAVASEHGPGRAEYLIQR
                     LLAHGGDIGLQIPHLVQTPYVNTIPLADEQARYWFPGDLDLERRIRAFIRWNAAVMVV
                     KANHVAEGIGGHLATFASSASLYDVGFNHFWRGKDDGNNGDSVYIQGHAAPGIYARAY
                     LEGRLDEIELDNFRREIGGNGLSSYPHPRLMPDFWEFPTVSMGLGPINSIYQARFNRY
                     LHHRRLEDTSELRVWAFVGDGECDEPETLGSIALAGRERLDNLTWVINCNLQRLDGPV
                     RGNGKIIQELEGVFRGAGWNVIKVVWGSAWDELIENDHDGLLVRKMNETVDGEFQRFT
                     VEDGAYIREHFFGPDPRLRKMVDHLSDEELRWLPRGGHDYRKLYAAYKAATEQRGAPT
                     VILAKTIKGWTLGADVEGRNATHQIKKLSNKQLRDLRLRLHLEEEIPEESLADGQEPP
                     YYRPAEGTPAHEYLMARRQALGGSMPKRVVRTTSPLTLPDDSVFDEFRSGSGKKAAST
                     TTAFTRMLRAFSRDPGFGRRVVPIIPDEARTFGMDSLFKEFGIYASEGQKYEPVDHKL
                     LLSYHESIDGQLLEEGITEAGSMASWTAAATSYATHGVPMVPFFIFYSMFGFQRVGDL
                     IWA"
BASE COUNT          292 a          694 c          766 g          347 t
ORIGIN      
        1 cggcccagat caggtcaccc acccgctgga agccgaacat cgagtagaag atgaagaacg
       61 gcaccatcgg cacgccgtgg gtggcgtacg aggtggcggc ggcggtccac gaggccatcg
      121 agcccgcctc ggtgatgccc tcctccagca gctggccgtc gatcgactcg tggtagctga
      181 gcagcagctt gtggtcgacg ggctcgtact tctggccttc cgacgcgtag atgccgaact
      241 ccttgaacag ggagtccatg ccgaaggtgc gggcctcgtc ggggatgatc ggcacgacgc
      301 ggcgaccgaa gccgggatcg cgggagaagg cccgcagcat gcgggtgaag gccgtggtgg
      361 tggacgccgc cttcttgccg gagccgctgc ggaactcgtc gaacaccgag tcgtcgggca
      421 gcgtgagcgg cgacgtggtg cgcacgacgc gcttgggcat cgagccgccg agcgcctgcc
      481 gccgggccat gaggtactcg tgggccggcg tgccctccgc cggccggtag tacggcggct
      541 cctgcccgtc ggccagggac tcctcgggga tctcctcctc caggtgcagc cggaggcgga
      601 ggtcccggag ctgcttgttc gacagcttct tgatctggtg ggtggcgttg cggccctcca
      661 cgtcggcgcc gagcgtccag cccttgatgg tcttggccaa gatgaccgtc ggggcgcccc
      721 gctgctcggt ggcggccttg taggcggcgt agagcttccg gtagtcgtgg ccgccgcggg
      781 ggagccagcg cagctcctcg tcggacaggt ggtccaccat cttccgcagc cgggggtccg
      841 ggccgaagaa gtgctcgcgg atgtaggcgc cgtcctcgac ggtgaaccgc tggaactcgc
      901 cgtcgacggt ctcgttcatc ttccggacga gcagcccgtc gtggtcgttc tcgatgagct
      961 cgtcccacgc cgagccccac acgaccttga tcacgttcca gccggcgccg cggaagacgc
     1021 cctcgagctc ctggatgatc ttgccgttgc cgcgcaccgg cccgtcgagg cgctggaggt
     1081 tgcagttgat gacccaggtc aggttgtcca gccgctcgcg gccggccagg gcgatggagc
     1141 ccagcgtctc gggctcgtcg cactcgccgt cgcccacgaa cgcccagacc cgcagctccg
     1201 acgtgtcctc caggcggcgg tggtggaggt agcggttgaa ccgggcctgg tagatcgagt
     1261 tgatgggccc gagccccatc gacaccgtcg ggaactccca gaagtccggc atgaggcgcg
     1321 ggtgcgggta gctggagagg ccgttgccgc cgatctcgcg ccggaagttg tcgagctcga
     1381 tctcgtcgag gcggccctcg aggtacgccc gggcgtagat gcccggtgcg gcgtggccct
     1441 ggatgtagac ggagtcgccg ttgttgccgt catccttgcc ccgccagaag tggttgaagc
     1501 cgacgtcgta gagcgaggcc gacgaggcga aggtggcgag gtggccgccg atgccctcgg
     1561 ccacgtggtt cgccttcacc accatcaccg cggcgttcca gcggatgaac gcacggatgc
     1621 gccgctcgag gtcgaggtcg cccgggaacc agtaccgggc ctgctcgtcg gccagcggga
     1681 tcgtgttcac gtacggcgtc tgcaccaggt gcgggatctg cagcccgatg tcgccgccgt
     1741 gggcgagcag gcgctggatc aggtactcgg cccgtcccgg cccgtgctcc gacgcgacgg
     1801 cgtcgagcga gtcgagccac tcgcgcgtct cgtccggatc ggcgtcgtac agctggccca
     1861 cgaacccgtc atcgatgccc attcgtccac tgtgccgcgt gccggggcgc ttcgcacccg
     1921 gctcgtcact cgttcgctgg cgctcgctgc gttcttcgaa cgtgaggacg ggctggcgcc
     1981 cggtggtctc gcagctcgga gaacacgcct cgctggtcgc ggcatagttg cgggatgggc
     2041 gaacggctgc gggtgtacac ggatggggcc tgctcgggga acccgggtcc gggcgggtg
//
LOCUS       DATMFS010000279         3206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_126435, whole genome shotgun sequence.
ACCESSION   DATMFS010000279 DATMFS010000000
VERSION     DATMFS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126435"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VNQ33_05125"
     CDS             complement(<1..336)
                     /locus_tag="VNQ33_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ97519.1"
                     /translation="MPRAYLDHASTSPLRPVAREAMVAALTELVGDPGRIHEEGMASR
                     VALEQAREQVAALLGARSREVVFTSGATESIAAAVSGAAARASSRGAVDGDRRTHQVV
                     TAVEHSAVRF"
     gene            657..1697
                     /locus_tag="VNQ33_05130"
     CDS             657..1697
                     /locus_tag="VNQ33_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97520.1"
                     /translation="MSAPGGSAYVAASEGKVLIGAQEQYGADGTVRQSAWFRVDAETG
                     GAEPIRLPSDKASVGPVIAVHDGFLLASRSCEEPVGDAGECFGNPELFFLGTRANTWT
                     PVEYPVELSGASAPRVFPVLDAARIGDETVIVASPTLGSQRHSSRMALSWADDRLSVK
                     KSLGPQRTPKHCIAGTSLVSLAQDGRQIIIEDLAGDAETETRPVPDGADTAFGGAPLN
                     LGCTDQGGLLFSRSSGVDGTVTVFPLAGGEAAKVAAITPNHYIVSIVSAPSEVVLSTT
                     GRDEPNRNDGAARVTSKLGQQALSAEIGNSYLTTDGATGAVYAVGPTESMVIAGEVEK
                     GYQTADIKIQKV"
     gene            1704..2642
                     /locus_tag="VNQ33_05135"
     CDS             1704..2642
                     /locus_tag="VNQ33_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97521.1"
                     /translation="MPSNHTATNHVPSKATGPGEPRRVSLVIFGFALIAVGLVAPAGP
                     AAAGTITGQNRDQSQIVQFNTYGGLGNATSGFNERINFDILDSIGSRNHKPNFLSLNE
                     VCEQGYNILVWVLTGTGSGYSTSNPSGYTFHHTVSLGQFFGGQIGSDLDDDCGAWYGN
                     ALLMRGAQVSGTGGEGWYSSSIQGPSSYGELRNWMCRKSAWSNTSYCSTHTTPLCRSD
                     STVPQIAAFRDIANYASSGGVYALGDLNCPPYLAGWAGAGFNEIEYPSPTGIIHHTTD
                     AGFKYDYIWKKNATFSAAPYIFEAARSDHHWVQAYP"
BASE COUNT          548 a         1083 c         1071 g          504 t
ORIGIN      
        1 gaagcggacc gccgagtgct cgacggcggt gaccacctgg tgcgtgcggc ggtccccgtc
       61 gaccgctcct cggctgctgg ctcgggcggc cgcaccgctg acggcggcgg cgatcgactc
      121 ggtcgccccg ctcgtgaaca cgacctcccg gctgcgggca ccgaggagcg ccgcgacctg
      181 ctcccgcgcc tgttcgagcg cgacccggga tgccatgccc tcctcgtgga tgcgcccggg
      241 gtcgccgacg agctcggtga gcgcggccac catcgcctcg cgcgcgacgg ggcggagggg
      301 ggaggtggac gcgtggtcga ggtacgcccg gggcacggtg cgaaccctac gaggtggcgc
      361 gccggacgaa gccgaagccg cgccggtcgc cggagccgtc ggtgaaggtg ctgtcgtgcc
      421 aggtgatccg cccgttgacg agcacgggcg acggaagcga cgaagcactg gtcgacagct
      481 cccacgagat gtgctacgac ggtcgaagta atcttcggag gttgaggatg aagcggtgga
      541 gctgtcgagc ggcgctgctc gtgctggttt gtgccgtctc tgcgtgcggt agcagcggtg
      601 gtgaagcggt cgactcaggg gctcagagcg gacctacccc gtggcaggat acggtcgtgt
      661 cggcacctgg cggcagcgcg tacgtcgctg cgtcggaggg caaggtgctc atcggcgcac
      721 aggagcagta cggggccgat gggaccgtga ggcaatcggc ttggtttcgc gtcgacgcag
      781 agaccggtgg tgcggagccg atccggcttc cctccgacaa ggcatccgtc ggcccggtga
      841 tcgcggtgca cgacgggttc ctgctcgcat cccggtcgtg cgaggagccg gtgggcgatg
      901 ccggagaatg cttcggaaac cccgagctct tcttcttggg cacccgagcc aacacgtgga
      961 cgcccgtcga gtacccagta gagctgtcgg gagcgtccgc accgagggtc ttccccgtcc
     1021 tcgacgcagc acgcatcggc gacgagaccg tcatcgtcgc ctcgccgacc ctgggctccc
     1081 agcggcacag ttctcgcatg gcgctgagct gggccgacga ccgcctctcg gtgaagaagt
     1141 ccctcggtcc gcagcgtacg ccgaagcact gcatcgccgg cacatcgctc gtgtcgctcg
     1201 cgcaggacgg acgccagatc atcatcgagg atctggccgg ggacgcggag accgagacgc
     1261 gcccggtacc cgacggcgca gacaccgcct ttggcggcgc ccccctcaac ttgggctgca
     1321 ccgaccaagg cggcctcttg ttctcccggt cctccggcgt ggacggcacc gtgaccgtct
     1381 ttccactggc cggcggcgag gcggcgaagg tcgccgctat cacccccaac cactacatcg
     1441 tgagcatcgt atccgcaccc tcggaagtcg tattgagcac cacgggtcgc gatgagccga
     1501 acaggaacga cggcgccgcg cgcgtcacct cgaagctcgg ccagcaggcg ctgtccgccg
     1561 agatcggcaa ctcctacctg accaccgatg gtgccaccgg cgccgtctac gccgtcggcc
     1621 ccaccgaatc catggtgatc gccggagagg tcgagaaggg ctaccagacc gccgacatca
     1681 agatccagaa ggtctgacgc aaggtgccga gcaaccacac agccacgaac cacgttccat
     1741 caaaggccac gggtccagga gaaccccgtc gtgtaagcct cgtcatcttc ggcttcgctc
     1801 tcatcgcggt cggacttgtt gcacccgcgg ggcccgccgc cgccggaacg atcaccggtc
     1861 agaaccgcga tcagtcccag atcgtgcagt tcaacacgta cggtggattg ggaaatgcca
     1921 ccagtggttt caacgagcga atcaacttcg acatcctcga ctcgatcggg tcgaggaacc
     1981 acaagcccaa cttcctgagc ctcaatgagg tctgcgagca gggttacaac atcctcgtgt
     2041 gggtccttac tggaaccggg tctggctaca gcacaagtaa cccatctggc tacacgttcc
     2101 accacaccgt cagcctcggg cagttcttcg gaggacagat cggatcagat ctcgacgatg
     2161 actgcggggc atggtatgga aacgcgctcc tcatgcgcgg ggcgcaggtg agcggaaccg
     2221 gcggtgaggg ctggtactcg tcgagcatcc agggtccgtc gagctacggc gagctccgca
     2281 actggatgtg ccggaagtcc gcatggtcca acacgagcta ctgcagcacc cacacaacac
     2341 cgctctgtag gtcggactcg acggtgcccc agatcgccgc cttccgcgat atcgccaact
     2401 atgcctcgtc cggcggcgtg tacgccctgg gcgacctcaa ctgcccgccc tatctcgccg
     2461 gatgggctgg agcaggtttc aacgagattg aatacccgtc tccaactggc atcattcacc
     2521 atacgactga cgccggattc aagtacgact acatctggaa gaagaacgcc acgttctcgg
     2581 ccgccccgta catcttcgaa gctgcacgtt cagaccacca ctgggttcag gcctacccct
     2641 gaaccgcgct ggcctggtgc cgagcagaag gtgctctccg agcgcacaga acccgggtcc
     2701 ccaccgccat catcagctct tccaccgacc cggcacctcg gcctcggcga gatcgctcgt
     2761 gaccgctcgg ggcccaccca gcggcggaac ggacggaggt cgtcatgaga cgccccggcg
     2821 ccagtggcag gaagctcgga gctcagatca cggcggttcc cagtgattcg gcggatgagc
     2881 tcttcaagac ccggaggtgt ccttgggggg gcatcggctt cgcccgacag caccgctcgg
     2941 gccgcggaga cggcgtcggc agggtcggac ttgccgtgac ggctccgctg ttggcggttc
     3001 gggcggttga ccttgagcgc ggccccgccg gcttcggcca gtggggcgag cccggcccga
     3061 ggcctgaacc ggctcggtct cagccggtga tggcggtgat gcaggtggcg tcgccgctgg
     3121 cccgggacgc ctcggtgagc gcggtggcat cgccgtagag ggtctcgagc atgccccgga
     3181 ccatgccgcg gtcgacggcg cagatc
//
LOCUS       DATMFS010000280         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_219276, whole genome shotgun sequence.
ACCESSION   DATMFS010000280 DATMFS010000000
VERSION     DATMFS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219276"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VNQ33_05140"
     CDS             complement(<1..1301)
                     /locus_tag="VNQ33_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWJ97522.1"
                     /translation="MPAPGEGPIVVVGGGAAATFLLHRMAADGTSRRPVVIVEPAERL
                     GPGIAYSTPDPVHLMNVCRIRLSVDGDGGQDLIGWLGERGVRADADTYLRRSMYGDYL
                     ADVAGRCADLVGVDHRRNRVAGLARDDRGWTVTLDDGAAVEAGDVVLATGFPGRPVPP
                     CDAPLDDPRVVADPWTPGVLDRPGIEEAVIIGTGLTMVDVALSLVERHPDVQIVAASR
                     RGLVPRTSRPGLPATIDPEVLPVPGMGGRDLVHHVIRLGRTQEDWQAVIDNVRPRAND
                     IWRELPYGEQGHILRRWFPWWNVHRHRTAPAVGARLAALLESGQLRIVRSRIASADGS
                     GAGDGRLTVRFARAGLPDERADLLVNAAGPSDDLSVSGDPVVQQLLHTGDVVIGPHRL
                     GLAVDRAGRAQGSGLGPTPGLWLVGALRRGTEWETTAVPEIR"
     gene            1673..2407
                     /locus_tag="VNQ33_05145"
     CDS             1673..2407
                     /locus_tag="VNQ33_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97523.1"
                     /translation="MDTLEAATESGDAINGLGTKFMLDLDTYVYGATLGFEGADFYLS
                     GRGGALGDVPAGVVSAAFVFWNPDFVAAGWDRTADVLPRRQAAQEFAGVCHRWAEEHM
                     GDDVDTARIAELAGRLNEAASPACAPLFAAWRSLDEPEPDRHKALALHRVNGLRELRG
                     ALHGAAVLATGVTPHAAVARRTPYMLGVFGWEEPHPDKEQVRAPWAEAQAATERSVAP
                     VFEALEPAERAEFVELVNALQASVND"
     gene            2788..3585
                     /locus_tag="VNQ33_05150"
     CDS             2788..3585
                     /locus_tag="VNQ33_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWJ97524.1"
                     /translation="MARPTWSGVISFGLVSVPVKAYTATRDKDVRFHRIERDTGARIK
                     QQNLSAATGEPVEDEDIVMGYELAPGKHVTFEREEIAAMRPDSTKAVQVLDFVDLDEV
                     DPVYFDKTYWLGPDGAAAVEPYELLLEAMEERNRVAIGAVVMRNKGRLAAIRPMGGLL
                     AMSTMRYADEVVASEDIDELPSERSDPEPRALDLALQVVDTMTSEWDPEHYPNTYNDV
                     FLDAIEARADGIVAEPAAEGEAEAPVIDLMAALRESVDAAKKKAAEG"
BASE COUNT          563 a         1397 c         1394 g          480 t
ORIGIN      
        1 cggatctcgg ggacggcggt ggtctcccac tcggtgccgc ggcggagcgc gcccacgagc
       61 cacaggccgg gggtcgggcc gaggccgctg ccctgggcgc ggccggcgcg gtcgacggcc
      121 agaccgaggc ggtgggggcc gatgacgacg tcgccggtgt gcaggagctg ctgcaccacc
      181 gggtcgccgg acaccgacag gtcgtcgctc gggccggcgg cgttgacgag gagatcagcg
      241 cgctcgtcgg gaaggcccgc acgggcgaag cggacggtga gccggccgtc tcctgcaccg
      301 gagccgtcgg ccgaggcgat gcggctgcgg acgatgcgga gctgaccgga ttcgagcagc
      361 gccgccaggc gggcgccgac ggccggggcg gtgcggtggc ggtgcacgtt ccaccacggg
      421 aaccagcggc ggaggatgtg gccctgctcc ccgtagggca gctcccgcca gatgtcgttg
      481 gcccggggtc ggacgttgtc gatgacggcc tgccagtcct cctgcgtccg tcccaggcgg
      541 atgacgtggt ggacgaggtc gcgcccaccc atccccggca ccgggaggac ctcagggtcg
      601 atggtggcgg gcagcccggg ccggctcgtc ctcggcacca ggccacggcg agaggcggcg
      661 acgatctgca cgtcggggtg gcgctcgacc agcgagaggg cgacgtccac catcgtcagc
      721 ccggtcccga tgatcaccgc ctcctcaatg cccggacgat cgagcacgcc gggcgtccac
      781 gggtcggcga cgacgcgagg gtcatcgagc ggcgcatcgc acgggggcac gggtcggccg
      841 gggaagcccg tcgccaacac gacgtcgccc gcctcgaccg cggcgccgtc atcgagggtc
      901 accgtccaac cgcggtcgtc gcgggccagg ccggcaaccc ggttgcggcg gtggtcgaca
      961 cccaccaggt cggcgcagcg gccggcgacg tccgccaggt agtcgccgta catggaccgc
     1021 cgcaggtagg tgtcggcgtc ggcgcggacg ccgcgctcgc cgagccagcc gatgaggtcc
     1081 tggcctccgt cgccgtcgac cgacagccgg atgcggcaca cgttcatcag gtgcaccggg
     1141 tccggcgtgc tgtacgcgat gccggggccg aggcgctcgg cgggctcgac gatcacgacc
     1201 ggccgccggg aggtcccgtc agccgccatc cggtgcagca ggaacgtcgc cgccgctccg
     1261 ccgccgacca ccacgatggg cccctcacct ggcgctggca ccggctgctc ccgtcactcg
     1321 tcgtgcacgc gaaggctagg ccagcaccgc caccgcccct gaccaggcgg ttgcgggtcg
     1381 aggcatccca ccgccgtcgc ccggcggggc gccgtggggc ccaacgcgac gaggacccac
     1441 ggaaggtgga ggaaacgtga ccgcatgcga ccgtcttcct ccaagttctg ccccccgctt
     1501 ccaacttggt ggatcgaggt cgccagcggc cgagatcctc caagttggaa gctggtggca
     1561 cagaccggta gcgggtcggt tgtcacacgc aacgcactgg tggcgggact gtgcgatgct
     1621 cccagcctcg tgatcgtgcg acacgagcac cggcaccagg gggaacaggc acatggacac
     1681 gctcgaagct gccaccgagt ccggcgacgc catcaacggg ctggggacca agttcatgct
     1741 cgacctggac acgtacgtgt acggggcgac gctcgggttc gagggcgcgg acttctacct
     1801 gtcgggccgg ggcggtgccc tgggcgacgt gcccgccggc gtcgtgtcgg cggcgttcgt
     1861 gttctggaac cccgacttcg tcgccgccgg atgggaccgc accgccgacg tgctgccccg
     1921 ccgccaggcc gcccaggagt tcgccggcgt ctgccaccgc tgggccgagg agcacatggg
     1981 cgacgacgtc gacaccgccc gcatcgccga gctcgccggc cggctgaacg aggccgcctc
     2041 gcccgcctgc gcaccgctgt tcgccgcgtg gcgcagcctc gacgagcccg agcccgatcg
     2101 ccacaaggcc ctcgccctcc accgggtcaa cgggctccgc gagctgcgcg gcgccctcca
     2161 cggtgcggcc gtcctcgcca cgggcgtgac gccccacgcc gccgtcgccc gccgcacgcc
     2221 ctacatgctc ggggtcttcg gctgggagga gccccacccg gacaaggagc aggtccgggc
     2281 gccgtgggcc gaagcccaag ccgccaccga gcgttcggtc gccccggtgt tcgaagcgct
     2341 cgagcccgcc gagcgagccg agttcgtcga gctcgtcaac gccctccaag ccagcgtcaa
     2401 cgactgacct cgctccccgc cgcgactccc ccgaggcgac gccgggcgga tcggcccacc
     2461 agcgggaccg gtcgacacga cgttcggacc gaggcgggga cccgaccggt acgtcgtgcg
     2521 gcttcagctc gttcgtgtgc cggatccacc cgaagttcgc cgcggaggcg agggcgaagg
     2581 cgagggcgaa ggcgagggcg aaggcgatgt ggtcggttgg ggcgggtcgt cggagggggt
     2641 gggcggcgtc gcctgccggc gtcgcctgag ggtgcgggca tcgccgccca tcgggtgggg
     2701 acaggtcgat ggctagcgtg gcctcaccgc ttctcccctc gcgaaaggcg ctccgatgcc
     2761 cgcaaccccc accccggccg gcctctgatg gcccgcccca cctggtccgg cgtcatcagc
     2821 ttcgggctgg tcagcgtccc ggtgaaggcg tacacggcca cgcgcgacaa ggacgtccgc
     2881 ttccaccgca tcgaacggga caccggtgct cgcatcaagc agcagaacct ctcagccgcc
     2941 accggcgagc cggtcgagga cgaggacatc gtcatgggct acgagctggc acccggcaag
     3001 cacgtcacgt tcgagcggga ggagatcgcg gccatgcggc ccgacagcac caaggcggtc
     3061 caggtgctgg acttcgtcga cctcgacgag gtcgatcccg tctacttcga caagacgtac
     3121 tggctcggac ccgacggtgc cgcggccgtc gagccgtacg agctgctgct cgaggcgatg
     3181 gaggagcgca accgagtcgc catcggcgcc gtcgtcatgc gcaacaaggg ccggctggcc
     3241 gccatccggc cgatgggcgg gctgctcgcc atgtcgacga tgcgctacgc cgacgaggtc
     3301 gtggccagcg aggacatcga cgagctcccc agcgagcgga gcgaccccga gccgcgtgcg
     3361 ctcgacctcg ccctccaggt ggtcgacacc atgacgtccg agtgggaccc cgagcactac
     3421 ccgaacacct acaacgacgt gttcctcgac gccatcgagg cccgggcgga cggcatcgtc
     3481 gccgagcctg ccgccgaggg cgaggccgag gctccggtca tcgacctcat ggccgccctg
     3541 cgcgagtccg tcgacgccgc caagaagaag gccgccgagg gctgaagctc agtccgcctt
     3601 gccccgacca ccgggatcgc ccgggcgatc ccggtggtcg gatcatcgag gtcctcgggc
     3661 tcggcccgca gctgggcgag gcggtcgtcc gacatccagg ccggcggttc gtcggcgatg
     3721 acgagctcgg gatagcgatc ggtgatctca tcgccagact gggcgagcac cacggcccac
     3781 aggccgcccg tgccgtagtc gtacgccacc aggaacgcac gcggctcgac tgcg
//
LOCUS       DATMFS010000281         3942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_56121, whole genome shotgun sequence.
ACCESSION   DATMFS010000281 DATMFS010000000
VERSION     DATMFS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56121"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VNQ33_05155"
     CDS             complement(<1..581)
                     /locus_tag="VNQ33_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ97525.1"
                     /translation="MAWEVEEFLASLTAVAAATTRAYRADLVDFTRWAGSAGLTGPDQ
                     VSRRDLRRYLADLSDRPLAPRTLARRASALRRYFGWQTRTARLAADPTLGLRAPRGTG
                     RLPKIVHEEQLHQILDEPPARAGLTDDQLADPRQRAVHLRDQAVLELLYGSGLRVSEL
                     CGLRQRDLDLLGRRITVWGKGSKQRMLPLSEPSV"
     gene            complement(642..1850)
                     /gene="dprA"
                     /locus_tag="VNQ33_05160"
     CDS             complement(642..1850)
                     /gene="dprA"
                     /locus_tag="VNQ33_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWJ97526.1"
                     /translation="MSAATPPGSAEARRELACAVALASLEGIGPATVLACLRGCGTEE
                     AWAAVVAGRATRIPALASALGRRSAREPGVEEHIARHAAAIDPGALLERHERAGQRVA
                     AYGTPGYPERLVGDVAPPAVVFWQGSLGGLDRPTVAIVGTRNATRLGFETASRLAVEL
                     VDRGVSVVSGLALGIDGAAHRAVVERRGGGVPIGVVATGLESTYPRRHQRLHRQVAAA
                     GLLLSESPIGSEPSRWRFPARNRIIAGLSDAVVVVESRSAGGSMLTAAEAVARDRTVL
                     AVPGHPTSAAAAGTLDLIADGAVPVRDVTDVLVAIGLGGQKPIAGPGAAPLAVPGAPV
                     SPLAASLLEHLDPNPMTLGELVLTSATGVDEASAALLELERAGLVTRSGAWFERARVV
                     GRPSGPAVVR"
     gene            complement(1847..3361)
                     /locus_tag="VNQ33_05165"
     CDS             complement(1847..3361)
                     /locus_tag="VNQ33_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWJ97527.1"
                     /translation="MGFASIPSAIVVGVDGHRVAVEVHAARGIPGLTIVGLPDASCRE
                     ARDRVRAAVLASGSKWTDQRVTVNLAPSSTRKVGSALDLAIAIAVLVASGELEEEQVR
                     GLAFVGELGLDGTVRPVPGIVPIIDALDADQVVVPVGSATDAELVEGPRVRAVATLRE
                     VLAALRTEEPWPDHDRPPNPVVVGASLDLSDVRGQPVIRKALEIAAAGGHHLLMIGPP
                     GAGKTMLASRLPGLLPALDRESAMEVSRIHSAAGHSLHGGLIRRPPLRSPHHTATMVS
                     LIGGGTDAMRPGEVSMAHAGVLFLDELAEFPPTVLDALRQPLEEGVIRVNRARFSAKL
                     PARFLLVAAMNPCPCGEAGRPGACRCPDGVLTRYHRRLSGPLLDRFDLRVDVLRPDVE
                     QLLGGEPGESSAAVARRVAAARARASARGVLANAHLRGSELERWAPVTTDAHDLLAET
                     LRAGRLSARGLDRVRRVARTIADLAGHDGPLTTAHLATALHLRADVRIGAGVLA"
     gene            3707..3934
                     /locus_tag="VNQ33_05170"
     CDS             3707..3934
                     /locus_tag="VNQ33_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97528.1"
                     /translation="MWPYADDEVEVRRIQPYQASKRYRCPGCQQDIPKGTGHVVAVPT
                     HAPDDRRHWHHGCWEHRHERRPGRTARAPRR"
BASE COUNT          541 a         1492 c         1464 g          445 t
ORIGIN      
        1 accgacggct cgctcagggg gagcatccgc tgcttggagc ccttgcccca caccgtgatc
       61 cgccggccca gcaggtcgag gtcgcgctgg cgcagcccgc acagctccga cacccgcagg
      121 ccgctcccgt acagcagttc gagcaccgcc tggtcccgca ggtggaccgc ccgctgccgc
      181 ggatcggcca gctggtcgtc ggtcagaccc gcgcgagcgg gcggctcgtc gaggatctgg
      241 tgcagctgct cctcgtggac gatcttcggc agccggccgg tgccacgcgg cgcccggagc
      301 ccgagcgtcg ggtccgcggc gaggcgcgcc gtcctcgtct gccacccgaa gtagcgccgg
      361 agggcagacg cccgtcgagc cagcgtccga ggcgcgagcg ggcggtcgct gaggtcggcc
      421 aggtagcgcc gcaggtcccg gcggctcacc tggtccggcc cggtgaggcc ggctgatccg
      481 gcccagcggg tgaagtccac gagatcggcc cggtacgcgc gggtggtggc cgctgccacc
      541 gccgtcaacg atgccaagaa ctcctcgact tcccaggcca cggcgccaga gtaccggtaa
      601 cgacgcgacc gcgaacggtg atggcgctcg ccttttgagg atcaccggac caccgccggg
      661 ccagacgggc gcccgacgac cctggcccgc tcgaaccacg cgccggaccg ggtcaccaga
      721 cccgcccgtt cgagctccag cagcgcagcc gaggcctcgt cgactccggt ggccgacgtg
      781 aggaccagct cgcccagcgt catcgggttg ggatcgaggt gctccaggag ggaggctgcg
      841 agcgggctca ccggtgctcc gggcaccgcg agcggcgccg cacccgggcc ggcgatgggc
      901 ttctggccgc ccaacccgat ggcgacgagc acatcggtga cgtcgcggac cgggaccgcc
      961 ccatcggcga tcagatcgag cgttccggct gccgccgccg acgtcgggtg gcccgggacc
     1021 gcgagcaccg tgcggtcccg ggccactgcc tccgcggccg tcagcatcga gcccccggct
     1081 gagcgggact cgacgacgac cacggcgtcg gagaggccgg cgatgatgcg gttgcgggcc
     1141 gggaagcgcc agcgggaggg ctcggagccg atcggcgact cgctcaggag gaggcccgct
     1201 gccgccacct gccggtgcag gcgctggtgg cgccgcgggt acgtgctctc caagcccgtg
     1261 gccacgaccc cgatcgggac cccgccgccc cggcgctcga cgacggccct gtgggcggcg
     1321 ccgtcgatgc ccaaggcgag ccctgacacc accgacacgc ctcggtcgac gagctcgacg
     1381 gcgagccggc tggcggtctc gaaccccagg cgggtggcgt tccgggtgcc gacgatcgcg
     1441 acggtcggcc ggtcgagacc gcccagcgag ccctgccaga acacgaccgc cggcggggcc
     1501 acgtcgccga ccagccgctc gggataccca ggggtcccgt aggcagcgac tcgctgcccc
     1561 gcgcgctcgt ggcgctcgag gagcgcgccc gggtcgatcg ctgccgcgtg gcgggcgatg
     1621 tgctcctcca caccgggctc ccgcgcgctg cgccgcccga gcgccgaggc gagggccggg
     1681 atccgcgtgg cccgaccggc gaccacggcc gcccaggcct cctccgtgcc gcagcctcgg
     1741 aggcacgcga gcaccgtggc cggaccgatg ccctccaacg acgccagcgc gacggcgcag
     1801 gccagctccc ggcgggcctc ggccgatccg ggtggggtgg cggcgctcat gccagcaccc
     1861 cggctccgat gcggacatcg gcccgcaggt gcagggccgt cgcgaggtgc gccgtggtca
     1921 gcggaccgtc gtgaccagcg aggtccgcga tcgtccgagc cacccgccgc acccggtcga
     1981 ggccccgggc gctcaagcgc ccagcccgga gcgtctcggc gaggaggtcg tgggcgtcgg
     2041 tggtcaccgg cgcccaccgc tccagctcgc tgccccgcag gtgggcgttg gcgaggacgc
     2101 cacgggcgct cgcccgggcg cgcgctgcgg cgacgcgtct ggccacagcg gccgacgact
     2161 cccccggttc tccaccgagc agctgctcga catcgggacg caagacgtcg acccggaggt
     2221 cgaaccggtc gagcaggggg cccgacaagc ggcggtggta ccgggtgagg accccgtcgg
     2281 ggcagcggca ggctccgggc cttcctgcct cgccgcacgg gcaggggttc atggcggcga
     2341 ccagcaagaa gcgcgccggc agcttggccg agaagcgagc ccggttcacg cggatcacgc
     2401 cttcctccag cggctggcgg agcgcatcga gcaccgtggg cgggaactcg gccagctcgt
     2461 cgaggaacag cacgccggcg tgggccatgg acacctcgcc gggccgcatc gcatcggtcc
     2521 cgccgccgat gagcgacacc atcgtcgccg tgtggtgcgg agaccgcagc ggtggtcggc
     2581 ggatgaggcc gccgtgcagc gagtgccccg ccgccgagtg gatccgcgac acctccatcg
     2641 ccgactcgcg gtcgagcgcg ggcagcaggc ccggcagccg gctggccagc atggtcttgc
     2701 ccgcgccggg cgggccgatc atcaagaggt ggtggccacc ggccgcggcg atctccagcg
     2761 ccttgcggat gaccggctgt ccgcggacgt cgctcaggtc gagcgacgct cccacgacga
     2821 ccgggttggg cgggcggtcg tggtccggcc acggctcctc cgtgcgcagc gccgcgagca
     2881 cctcgcggag ggttgcgacc gcacggaccc ggggcccctc gaccagctcc gcatccgtcg
     2941 ccgagccgac gggcaccacc acctggtcgg cgtcgagcgc atcgatgatg ggcacgatcc
     3001 cgggtaccgg ccgcacggtg ccgtcgagcc cgagctcacc gacgaacgcg aggccgcgaa
     3061 cctgttcctc ctcgagctcg ccggacgcga cgaggacagc gatggcgatg gcgaggtcga
     3121 gcgcgctccc caccttccgg gtggaggacg gcgccaggtt cacggtgacc cgctggtcgg
     3181 tccacttcga cccgctcgcg agcacggccg ctcgcacgcg gtcgcgcgcc tcgcggcacg
     3241 acgcgtcggg caagccgacg atggtcaggc ccgggatccc ccgggcagcg tggacctcca
     3301 cggcgacgcg gtggccgtcg acgccgacga cgatggcaga tgggatggat gcgaagccca
     3361 cgacggctcc tcctcgcccc gcacggagcg gaccaggggt cagggaggga cgtcgttgcc
     3421 gccgacggtc gggcggggcg gcgcttcgcc gcggggccgt ccaccgacgg cgaccagcac
     3481 cctacccacg aggggtgacg gcgagcaccg cctgcgccgc atcagcagca caccgggcac
     3541 cgagcgccga ccgggcgatg cgtcggcgga cgagctggtc cccgtaacct cgggccatgg
     3601 gcttcaaccc gcaccggaag atggccaaga cgcctgcgga ctacgccatg gtcatcggcg
     3661 ccgtcgtggt gtgcgtgctg ctgctcgcct gggccttcct cggctgatgt ggccctacgc
     3721 cgacgacgag gtcgaggttc ggcgcatcca gccgtaccag gcgtcgaagc ggtaccggtg
     3781 ccccggctgc cagcaggaca tccccaaggg cacgggccac gtggtcgccg tgccgaccca
     3841 cgcccccgac gaccggcgcc actggcacca cggctgctgg gagcaccgcc acgagcggcg
     3901 gccggggcgt acggcccgag cgccgcgccg ctagcgccgt ca
//
LOCUS       DATMFS010000282         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_241394, whole genome shotgun sequence.
ACCESSION   DATMFS010000282 DATMFS010000000
VERSION     DATMFS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241394"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VNQ33_05175"
     CDS             complement(<1..281)
                     /locus_tag="VNQ33_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97529.1"
                     /translation="MSSRTTAPPSMDWQASLFGDAEPVPDWAFSTVERVELGDGAWVD
                     IAQDWMSGADRLFERLLTTVTWREREVPMYEKVVPEPRLSHWWGRDGERA"
     gene            complement(444..1679)
                     /locus_tag="VNQ33_05180"
     CDS             complement(444..1679)
                     /locus_tag="VNQ33_05180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWJ97530.1"
                     /translation="MSSSTGPLAGVRIIESSMLGPAAITTQLADLGAEVIKVEAPSGD
                     YIRQMTWPIVEGVSLMHLHLNRGKKSITLDLRTPEAVQTYLDLVRDADVVIEAMRPGG
                     LERRGLGYDKLREVNPAIVFCTISGYGMTGPYAELPSHGVAYDVWAGLVKPEITEDGF
                     AAIPEHPSMGIHAGPLFGALGVLAGVIKARATGEGCRLEIAQSDAAAAMDWYRSESWK
                     AYERPESEVTGNASDNYVRRAPGTAGMRDGVRYQFYEASDGAFVLLQASEREFWENFC
                     RGIGRPELFEQNPGAKFADHAVGNVELRRQLKEIFSTRPAQEWVDLGLEINVPIINVN
                     TPQTLKDDPQFQARFEWIPAERLGAEQQPSPIKYLDLDIPLPEKAPTVGQHTDEVLRD
                     LLGKSDDDIAKLREHGALG"
     gene            complement(1818..2366)
                     /locus_tag="VNQ33_05185"
     CDS             complement(1818..2366)
                     /locus_tag="VNQ33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97531.1"
                     /translation="MSKRLLAALLAVLAATFALTACGSSAGDNADTPAAKDTGTDGEK
                     SKSQEETDKAESCDGFTAGADDVVRTFCDGTAKVDLTVGGDDYTLSGGDCATEGGYFT
                     VNIGVVTDSGFSGTKPDYFGSNMPEADGDYGESDQAFATFAVDGQEYSVPKTTGTHDA
                     DSATFSGTTTDGDIAVTGTVTC"
     gene            2518..>2780
                     /locus_tag="VNQ33_05190"
     CDS             2518..>2780
                     /locus_tag="VNQ33_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97532.1"
                     /translation="MGTVDFDVFDADNHYYEAPDAFTRHLDPRMQKRTMQWADVDGRT
                     RLVVGGRINRFIPNPQFDPVARPGCLDDYFRGRSPADDIRGAFG"
BASE COUNT          386 a          901 c         1033 g          460 t
ORIGIN      
        1 gcccgctcgc cgtcgcggcc ccaccagtgg gagagccggg gctcgggcac gaccttctcg
       61 tacatcggca cctcgcgctc acgccacgtg accgtggtga gcagccgctc gaagagcctg
      121 tccgccccgc tcatccagtc ctgggcgatg tcgacccacg ccccgtcgcc cagctccacc
      181 cgctcgacgg tcgagaacgc ccagtccggc acgggctcag catcgccgaa cagcgatgcc
      241 tgccaatcca tcgacggcgg agcggtggtg cgcgacgaca cctggccatg ctagcgaccg
      301 gaatcgaaca ggtgttcgct tccacgattt cgggtcacct gggcacctca gacgtgctca
      361 ggtgacccga aatcgccggt tgttcgcctt tcgggtcggc tgtgcaccgg ggcggtgccc
      421 aggcgacccg aaatcgggtg ggttcagccg agggcgccgt gctcgcggag cttggcgatg
      481 tcgtcgtcgg acttgccgag gaggtcgcgc aggacctcgt cggtgtgctg gccgacggtg
      541 ggggccttct cgggcagcgg gatgtccaag tcgaggtact tgatgggcga cggctgctgc
      601 tcggcgccca ggcgctcggc cgggatccac tcgaaccggg cctggaactg cggatcgtcc
      661 ttgagcgtct gcggcgtgtt gacgttgatg atggggacgt tgatctcgag cccgaggtcc
      721 acccactcct gggccgggcg ggtcgagaag atctccttga gctgccggcg gagctcgacg
      781 ttgccgacgg cgtggtcggc gaacttggcg ccggggttct gctcgaacag ctcgggccgg
      841 ccgatgcccc ggcagaagtt ctcccagaac tcgcgctcgc tggcctggag caggacgaag
      901 gcgccgtcgg atgcctcgta gaactggtag cggacgccgt cgcgcatgcc ggcggtgccc
      961 ggcgcccggc gcacgtagtt gtccgaggcg ttgccggtga cctcggactc ggggcgctcg
     1021 taggccttcc agctctcgga gcggtaccag tccatggcgg cggcagcgtc ggactgggcg
     1081 atctcgaggc ggcagccctc gcctgtggcg cgtgccttga tgaccccggc cagcacgccc
     1141 agcgcaccga acaggggacc ggcgtggatg cccatcgacg ggtgctcggg gatggcggcg
     1201 aagccgtcct cggtgatctc gggcttcacg agcccggccc acacgtcgta ggcgacgccg
     1261 tggctgggca gctcggcgta ggggccggtc atgccgtagc cggagatggt gcagaacacg
     1321 atggccgggt tgacctcgcg gagcttgtcg tagcccaggc cgcggcgctc gaggccgccg
     1381 gggcgcatgg cctcgatcac cacgtcggcg tcgcggacca agtcgaggta ggtctgcacg
     1441 gcctcggggg tgcgcaggtc caaggtgatg gacttcttgc cccggttgag gtggaggtgc
     1501 atcagcgaga cgccctccac gatgggccac gtcatctggc ggatgtagtc gcccgagggc
     1561 gcctcgacct tgatgacctc ggcgccgagg tcggcgagct gggttgtgat ggcggcgggg
     1621 ccgagcatcg agctctcgat gatgcggacg cccgcgaggg ggccggtgct ggaagacatg
     1681 gtgacctcgg gctgtcgaat ctgacggtcc gtcagattag cgttccgtcc gaggtcggcc
     1741 cacgctcggc gcagccccac gcgtggggat gggcgcggtg gtccaccgac gcgatggcgg
     1801 gtgcacgcac ccgtcggtca gcaggtgacg gtgccggtga ccgcgatgtc gccgtcggtg
     1861 gtggtgccgg agaacgtggc gctgtcggcg tcgtgggtgc cggtcgtctt cggcaccgag
     1921 tactcctggc cgtcgacggc gaacgtggcg aacgcctggt cgctctcgcc gtagtcgccg
     1981 tcggcttcgg gcatgttcga gccgaagtag tcgggcttcg tgcccgagaa gccggagtcg
     2041 gtcacgacgc cgatattgac ggtgaagtag ccgccttcgg tggcgcagtc accgccggac
     2101 agtgtgtagt cgtcgccgcc gaccgtgagg tcgaccttgg cggtgccgtc gcagaaggtg
     2161 cggacgacat cgtccgcgcc ggccgtgaac ccgtcgcagc tctcggcctt gtcggtctcc
     2221 tcctgcgact tggacttctc gccgtcggtg ccggtgtctt tcgctgcggg ggtgtcggcg
     2281 ttgtcgccgg cgctgctccc gcaggcggtc agtgcgaagg ttgcagcgag gacggcgagc
     2341 agggcggcga gcaggcgctt cgacatcggt ggctccttgg gggtggggtg gcggcgcgat
     2401 cgtaggtctg cgaccggcgc cgaggaggga gcgagcagga tcggcgcgcc gggggtaggt
     2461 tgaaaacgag aacacgttct cgtttcgtcg tcatccacca gcaggcaggg ggccgccatg
     2521 ggcacggtcg acttcgacgt cttcgacgcc gacaaccact actacgaagc tcccgatgcc
     2581 ttcacccgcc acctcgaccc gcggatgcag aagcggacca tgcagtgggc cgatgtcgac
     2641 ggcaggaccc gcctcgtggt gggcgggcgc atcaaccggt tcatcccgaa cccgcagttc
     2701 gacccggtgg cgcggcccgg ctgcctggac gactacttcc ggggccgatc gccggccgac
     2761 gacatccgcg gcgcgttcgg
//
LOCUS       DATMFS010000283         8840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_219382, whole genome shotgun sequence.
ACCESSION   DATMFS010000283 DATMFS010000000
VERSION     DATMFS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219382"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1460
                     /locus_tag="VNQ33_05195"
     CDS             186..1460
                     /locus_tag="VNQ33_05195"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HWJ97533.1"
                     /translation="MATAAAALAAAAVVIGSPVSSRPAGKAAPAAVAGVDDDVHLNEI
                     QVVGSHNSYKKMVSDKEEALRRQFIDGAADLMQYQHEPLGTQFQSRKVRQVEIDIYLD
                     SAGGLYSNPLLRSAAGVGPYDPAMNEPGIKVLHIQDVDYASTCLTFVDCLTQMKTWSD
                     ANPSHVPIAVLVEFKDDELDFPGFTFTSPEKFTAANIGTVDDEIRSVMDADDLITPDD
                     VRGSHATLDEAVTTDGWPTLGASRGKFLFLMDNKGGYRTNYLSGHPNLEGRVLFTNAD
                     PGDPDAGFVERNDSSDSSIPGLVKAGYVVRTRADGDTKEARANDTGPRDAALASGAQW
                     VSTDYEGPGLAVGFTTPYSVEIPGGTVARCNPVNAPPGCDSALLDTIYTPLAPPDWST
                     TTSTTTTTTTVVGSDPEAEGATPVPGEASYTG"
     gene            1541..1954
                     /locus_tag="VNQ33_05200"
     CDS             1541..1954
                     /locus_tag="VNQ33_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97534.1"
                     /translation="MLWERDGVFVLAASQALVDVHEVLAWVVIVANGLAGSWALAAHK
                     DERFRVRQLWWFTALAELAVFVQVALGVVILQSHKGEDYGFHVLYGFSAAFAVGIIYS
                     YRGQLKEKRYLLYGFGGLFIMGLGLRLLTIGQGAI"
     gene            complement(1959..2420)
                     /locus_tag="VNQ33_05205"
     CDS             complement(1959..2420)
                     /locus_tag="VNQ33_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HWJ97535.1"
                     /translation="MKTRLASLALVALVLVGAGSLTACTPQLNAVEAAREQIGQPYKS
                     GGESRAEGGFDCSGLTFYAWKQAGVTLPRSSSAQYAWATKINKADLQPGDLVFYSSSK
                     SASNISHVALYAGNGKIIQAHKPGVPASEDDLATWWTSNLIGYGRVPASAL"
     gene            complement(2615..2851)
                     /locus_tag="VNQ33_05210"
     CDS             complement(2615..2851)
                     /locus_tag="VNQ33_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWJ97536.1"
                     /translation="MAVTAYILIQTEVGKAASVASEVREIPGVVAADDVTGPYDVVVR
                     VEAPSMDELGRLVVSRVQMLAGITKTTTMPVVNL"
     gene            complement(3066..4862)
                     /locus_tag="VNQ33_05215"
     CDS             complement(3066..4862)
                     /locus_tag="VNQ33_05215"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWJ97537.1"
                     /translation="MTATTSNEKLKAWVAEWAEVFGPTDVHWCDGSAEEYESLCRLLV
                     DGGTFEKLSDAKRPNSYLALSDPGDVARVEDRTFICSAKEVDAGPTNNWREPAEMKET
                     LLGHYRGAMAGRTLYVVPFSMGPLGSPIAHIGVQLTDSPYVAVSMRIMTRMGQGALDV
                     LGDGDWVKCLHSVGYPLTDGRADVPWPCDADNKYITHFPETNEIWSYGSGYGGNALLG
                     KKCFALRIASTMARDQGWMAEHMLIVGITNPAGEKRYVAGAFPSACGKTNMAMLIPTL
                     PGWTCETIGDDIAWMKFGEDGRLYAINPEAGFFGVAPGTGDDTNPNAIASLHANSIFT
                     NVAKTDDGDVWWEGLTNETPAHLIDWKGNDWTPESGVPAAHPNARFTAPAEQCPSIDP
                     KWEDPAGVPISAILFGGRRATNVPLVTEAFDWQHGTFLGSIMSSEKTAAAAGTIGEVR
                     FDPFAMLPFMGYNAGDYMQHWLDIGKATDADKLPKLFWVNWFRKGDDGSFLWPGFGEN
                     SRVLKWVLERVSGEAEAVDTAIGRVPLPDGIDTTGLDSVPTETLAELLKVDNEAWRGE
                     IPLIEEHFAFIGEHLPSELRDELQELEKRLAN"
     gene            complement(4908..5162)
                     /locus_tag="VNQ33_05220"
     CDS             complement(4908..5162)
                     /locus_tag="VNQ33_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97538.1"
                     /translation="MEVVVVQGVIKSYDPGTGDGIIMCDTDLADYDLAPGSIHGSPFR
                     MLRQGQRVIFDLDDSGRATGLRLGSEVDMGTPGFGPAPEV"
     gene            complement(5363..5575)
                     /gene="rpmB"
                     /locus_tag="VNQ33_05225"
     CDS             complement(5363..5575)
                     /gene="rpmB"
                     /locus_tag="VNQ33_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWJ97539.1"
                     /translation="MASTCEVCGKKPSFGMSVSHSHRRTKRRWSPNIQQVRAVVAPGT
                     VRRVNVCTGCLKAGKVTKATRAKTSA"
     gene            5727..7394
                     /locus_tag="VNQ33_05230"
     CDS             5727..7394
                     /locus_tag="VNQ33_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HWJ97540.1"
                     /translation="MTTLERLGADDLRLVVAAFRDALQAHKDGINRLNVYPVPDGDTG
                     SNMALTITSVATELEGVDGDLAAVCKAIGHGALMGARGNSGVILSQILRGLTQTLATA
                     ADGEVDAALLAQGLEVAAKGAYEAVGNPVEGTILTVIRVASEGASAAAGAGGSLVEVL
                     DAARTSGHEALANTPEQLEVLKAAGVVDAGGTGLMLLIDGFLHVVDDRELPAASVTEA
                     VADAGHVEDLQILAHGDDGHERGLADLRYEVMYILEAPDAAIPAFKDVWAGIGDSIVV
                     VGGDGLFNCHIHTDDIGPAIEAAIEIGKPSKIRVTDLLEEVEEERWVREAEEHPEPEP
                     DAAPVATAVVAIATGDGVKRIFHSLGARVLTGGQSMNPSTAQILEAVEAAPSDCVVIL
                     PNNKNIIAVAEQVDATTDKTVVVVPTKGIPEGFAALVAYDPDAEIAENASGMADAAAN
                     VLAGEVTQAVRDSSCDVGPIVEGDFLGIARDGIQAVAPSVSEATIGLLDHLLTEDHEI
                     VTLITGEGSSDAETRKVLLWIEEHHDGVETETHHGGQPLYPYFVGIE"
     gene            7404..>8840
                     /locus_tag="VNQ33_05235"
     CDS             7404..>8840
                     /locus_tag="VNQ33_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97541.1"
                     /translation="MADDEGDEVVTPITGEGHRNEADGPAGRPVRRPRQVTSLAGFDD
                     PPPPLHALVRSWPWAGWLVAAVVALRLVAVAGMRLYLYVDSAEYDRIDFSGDWRRPWA
                     TPCLYWLIPGRARGLVVGQALVGAVCWAALALSAAAWFRFRTSRIVVAAVLAGLGCTT
                     IVTNWDAAKLSESLGLSLTVLVIASWLNLIRRTEVGTALFVALATLPWLFVRQSLIPT
                     AWLVVGLAALVAAITWRRRGSEGLAVRVLAALCLVLAAETAVATVTYSHNQEIVQENL
                     RVIISNRVASDPARLVWFTDHGMPAPASGRFDPETLRSDPAFSTWVAHEGPSTYLRYL
                     LTHPWYTATAPLDDFTDVQRSTADAVEPAAAMLSPPDRYAASRPVIPSVVEDVIFGAG
                     DTGTVIFLLVLVGGWTLARIRRRSARWAIPWALVGLAITSLYTGWHGATPELGRLALL
                     GAVTLRIGLYLQLAVLIEDEVVGRRRPVV"
BASE COUNT         1271 a         3036 c         3176 g         1357 t
ORIGIN      
        1 tgttcgcccc cggctacgcc tcggcgaccg tgatcgacgt cgatccggcg tccggcctga
       61 tctcggtggt ggagcgcgga tcggaggcga cccccgaggt ggggtgaccg gcgcgctacc
      121 gtcgtctccg gttcatcttg agttcgggga gcgttcacca catgggtcac cgtcggttcg
      181 tcgcattggc cacggctgca gccgcactcg ctgcggccgc cgtcgtcatc gggtctcccg
      241 tcagcagccg gcctgccggg aaggcagccc ctgctgcggt cgcgggcgtc gacgacgacg
      301 tgcacctgaa cgagatccag gtggtcggca gccacaactc gtacaagaag atggtgtcgg
      361 acaaggagga ggcgctgcgc cggcagttca tcgacggcgc cgccgacctc atgcagtacc
      421 agcacgagcc gctcgggacg cagttccaga gccgcaaggt ccgccaggtc gagatcgaca
      481 tctacctaga ctcggcgggc ggcctctact cgaacccgct cctgcggagc gccgccggcg
      541 tcggcccgta cgacccggcc atgaacgagc cgggcatcaa ggtgctgcac atccaagacg
      601 tcgactacgc cagcacctgc ctcaccttcg tcgactgcct cacgcagatg aagacgtgga
      661 gcgatgcgaa cccgagccac gtgcccatcg ccgtgctggt cgagttcaaa gacgacgagc
      721 tcgacttccc gggcttcacc ttcacctcgc ccgagaagtt cacggccgcc aacatcggca
      781 ccgtcgacga cgagatccgc tcggtcatgg acgccgacga cttgatcacc cctgatgacg
      841 tgcggggcag ccacgcgacg ctcgacgagg cggtcaccac cgatggctgg cccacgctcg
      901 gcgcgagccg cggcaagttc ctcttcctga tggacaacaa gggcggctac cgcaccaact
      961 acttgagcgg gcatcccaac ctcgagggcc gggtcctgtt cacgaacgcc gatccgggtg
     1021 acccagacgc cggcttcgtg gagcgcaacg actcgtcgga ctcgagcatc cccggtctcg
     1081 tgaaggcggg ctacgtggtc cgcacccggg ccgacggcga caccaaggag gcccgcgcca
     1141 acgacacggg tcctcgggac gcggcgctcg ccagcggcgc ccagtgggtg agcaccgact
     1201 acgaaggccc ggggctcgcc gtcgggttca ccacgccgta ctccgtcgag atccccggtg
     1261 gcacggtcgc acgctgcaac ccggtgaacg cgccgcccgg ctgcgactcg gcgctgctcg
     1321 acacgatcta caccccgctc gccccgccgg actggagcac cacgacgtcg accaccacca
     1381 ccacgaccac cgtcgtgggc tccgatcccg aggccgaggg cgcgacgccg gttccgggtg
     1441 aggccagcta caccggctga tccccaggat cgggccacgg ggttgatcgt cacccggtcg
     1501 gagacggcgg cggcttcggc ccacttcgca cggcggcctc gtgctgtggg agcgtgacgg
     1561 cgtgttcgtg ctcgctgcct cgcaggcgct ggtcgatgtc cacgaggtcc tggcctgggt
     1621 ggtcatcgtg gcgaacggcc tggccggcag ctgggccttg gccgcccaca aggacgagcg
     1681 gttccgggtc aggcagctct ggtggttcac cgcgctcgcg gagctcgccg tgttcgtcca
     1741 ggtggcgctc ggcgtcgtga tcctgcagtc ccacaagggc gaggactacg ggttccatgt
     1801 gctgtacggg ttctcggcgg ccttcgcggt cgggatcatc tacagctacc gcggccagct
     1861 gaaggagaag cggtacctgc tctacggctt cggcggcctc ttcatcatgg ggctgggcct
     1921 gcgcctgctc accatcgggc agggcgcgat ctgatgtctc agagggcgct ggccggcacc
     1981 cggccgtagc cgatgaggtt gctggtccac cacgtggcca ggtcgtcctc gctggcgggc
     2041 acgccgggct tgtgggcctg gatgatcttg ccgttgccgg cgtagagcgc cacgtggctg
     2101 atgttcgacg ccgacttgga gctgctgtag aacacgaggt cgccgggctg gaggtcggcc
     2161 ttgttgatct tcgtggccca ggcgtactgg gcgctggagg agcgggggag ggtcacgccg
     2221 gcctgcttcc atgcgtagaa ggtgagcccg gagcagtcga acccgccttc ggcgcgcgac
     2281 tcgccgccgc tcttgtacgg ctggccgatc tgctcccggg cggcttcgac ggcgttgagc
     2341 tgcggggtgc aggcggtcag gcttccggcg ccgacgagga cgagggcgac gagggcgagc
     2401 gaggcgaggc gggtcttcac gagaacgggt tcctgggacg gagacagaga cggtgcgtca
     2461 gttcagtaac gacaagatga cgctgcgatc gagaaaccgt aacgaaaccg caatgttgag
     2521 tccagtcggg cgacgcgcac cctcgccgtc gccctgctgc caggcgcgtc gaaacgagcc
     2581 ctgggcacgg cggggcccag ggcaggtggt ggcgctagag gttgaccacc ggcatcgtcg
     2641 tggtcttggt gatgccggcc agcatctgca cgcggctgac gaccaagcgg cccagctcgt
     2701 ccatcgacgg ggcctcgacg cgcaccacca cgtcgtaggg gccggtcacg tcgtcggcgg
     2761 cgaccacccc ggggatctcc cggacctcgg aggcgaccga cgcggccttg ccgacctcgg
     2821 tctggatgag gatgtacgcc gtcacggcca tggctgcctc ctgggcgctc gtgctgggat
     2881 cagcaaccta acaacgacgg cagggccagg tcggcagtgg gaacggaacg tcgccggcgg
     2941 caccgccgca ttcggcgctg cggccacgac gttcggagcg ggcgggtgga ggaggggcga
     3001 gcggctcccg ggaacggcga aggccgcccc ggagggcggc cttcggtcac gtcaggaagc
     3061 ggggctcagt tggcgaggcg cttctcgagc tcctggagct cgtcgcggag ctccgagggg
     3121 aggtgctcgc cgatgaaggc gaagtgctcc tcgatcagcg ggatctcgcc ccgccaggcc
     3181 tcgttgtcga ccttgagcag ctcggcgagg gtctcggtgg ggacgctgtc gaggcctgtg
     3241 gtgtcgatgc cgtcgggcag cgggacccgg ccgatggcgg tgtcgacggc ctcggcctcg
     3301 ccgctgacgc gctcgaggac ccacttgagg acccggctgt tctcgccgaa cccgggccag
     3361 aggaacgagc cgtcgtcgcc cttgcggaac cagttgaccc agaacagctt cgggagcttg
     3421 tcggcgtcgg tggccttgcc gatgtcgagc cagtgctgca tgtagtcgcc ggcgttgtag
     3481 cccatgaacg gcagcatggc gaaggggtcg aagcggacct cgccgatggt gccggcggcg
     3541 gcggccgtct tctcggagct catgatcgag ccgaggaagg tgccgtgctg ccagtcgaag
     3601 gcctcggtga ccagcgggac gttggtggcg cggcggccgc cgaagaggat ggccgagatc
     3661 ggcacgccgg cggggtcctc ccacttgggg tcgatcgacg ggcactgctc ggccggtgcg
     3721 gtgaagcggg cgttcgggtg ggcggcggga acgccggatt cgggcgtcca gtcgttgccc
     3781 ttccagtcga tgaggtgggc gggggtctcg ttcgtgaggc cctcccacca cacgtcgccg
     3841 tcatcggtct tggccacgtt ggtgaagatc gagttggcgt ggagcgaggc gatggcgttc
     3901 gggttggtgt cgtcgccggt gccgggcgcc acgccgaaga agccggcttc ggggttgatg
     3961 gcgtagaggc ggccgtcttc gccgaacttc atccaggcga tgtcgtcgcc gatggtctcg
     4021 cacgtccagc ccggcagggt ggggatgagc atggccatgt tggtcttgcc gcaggccgac
     4081 gggaaggccc cggcgacgta cctcttctcg ccggccgggt tggtgatgcc gacgatgagc
     4141 atgtgctcgg ccatccagcc ctggtcgcgg gccatggtgg aggcgatgcg cagggcgaag
     4201 cacttcttgc cgagcagggc gttgccgccg tagcccgagc cgtagctcca gatctcgttg
     4261 gtctcgggga agtgggtgat gtacttgttg tccgcatcgc acggccacgg cacgtcggcg
     4321 cggccgtcgg tgagcgggta gcccaccgag tggaggcact tcacccagtc gccgtcgccg
     4381 aggacgtcga gggcgccctg gcccatgcgg gtcatgatgc gcatgctcac ggccacgtag
     4441 ggcgagtcgg tgagctggac cccgatgtgg gcgatgggtg agccgagcgg gcccatggag
     4501 aacggcacca cgtacagggt gcggccggcc atggcgccgc ggtagtggcc gaggagggtc
     4561 tctttcatct cggcgggctc acgccagttg ttggtggggc cggcgtcgac ctccttggcg
     4621 gagcagatga aggtgcggtc ctcgacgcgg gcgacatcgc ccgggtccga gagggcgagg
     4681 tagctgttgg ggcgcttggc atccgacagc ttctcgaagg tgcctccgtc gacgaggagc
     4741 cggcagagcg actcgtattc ctcggcggat ccgtcgcacc agtggacgtc ggtgggtccg
     4801 aagacctccg cccactcggc gacccacgcc ttgagcttct cgttgctggt ggtggcagtc
     4861 atgatctctc cggtgaccgg gcggaaaagc cgcaggtttt ggttgcttca gacttcgggt
     4921 gccggaccga agcccggcgt gcccatgtcg acctcggacc cgaggcgcag ccccgtggcc
     4981 cgaccgctgt catcgaggtc gaagatcacc cgctgcccct ggcggagcat gcggaacggt
     5041 gagccgtgga tcgacccggg agccaggtcg tagtcggcca gatcggtgtc gcacatgatg
     5101 atcccgtcgc ccgtccctgg gtcgtacgac ttgatcacac cttgcacaac aacgacctcc
     5161 acctgtggat gcggtgtcgc gaccctagtg ccgaggtccg atgtgacgca caaccaacgg
     5221 gccctcacgc catacgaagt tgggatgccg ttcatcggtt ctggcgatgc ggcgaggtca
     5281 gggcatcggc aactgcgatg gggcgcgacg aaggcgccgg gaagcccggc gccttcgtgt
     5341 cagaacggag cgaccgaagc ggtcaggcgg aggtcttggc ccgggtggcc ttggtgacct
     5401 tgccggcctt caggcagccg gtgcacacgt tgacccggcg cacggtgccg ggcgccacga
     5461 cggcgcgcac ctgctggatg ttcgggctcc accgccgctt ggtgcgacgg tgcgagtggc
     5521 tcacgctcat gccgaacgac ggcttcttgc cgcacacttc acacgtcgag gccatcactg
     5581 cactccaggg ttcgctgctg atcgacccgg actgggccga gggacaacgg tacgcggtcc
     5641 caccgggcgg tcacaagccg agcggccccg tcggtgacgc atccgtcgtc tgaccggccg
     5701 cggcgcagtc ggtaggctgc gccgccatga ccacgctcga gcgcctcggt gccgacgatc
     5761 tccggctcgt cgtcgccgca ttccgagacg ccctgcaggc gcacaaggac ggcatcaacc
     5821 gactcaacgt ctaccccgtc cccgatggcg acaccggcag caacatggcg ctcaccatca
     5881 cctcggtcgc gaccgagctc gagggtgtcg atggcgacct ggcggccgtg tgcaaggcca
     5941 tcggccacgg ggccctcatg ggcgcccgcg gcaacagcgg cgtcatcctc tcccagatcc
     6001 tgcggggcct cacccagacc ctcgccaccg ccgccgacgg cgaggtcgac gccgccctcc
     6061 tggcccaggg cctcgaggtg gcggccaagg gcgcgtacga ggcggtgggc aaccccgtcg
     6121 agggcacgat cctcaccgtc atccgcgtcg catcggaggg agcgtcggcc gcggccggcg
     6181 ccggcggctc gctcgtcgag gtgctcgacg ccgcccgcac gtccgggcac gaggcgctgg
     6241 ccaacacgcc tgagcagctc gaggtcctga aggccgcggg cgtcgtggat gccggcggca
     6301 ccgggctgat gctgctgatc gacggcttcc tccacgtggt ggacgaccgc gagctcccgg
     6361 cggcgtccgt caccgaggcg gtggccgacg ccggccacgt cgaggacctg cagatcctcg
     6421 cccacggcga cgacggccac gagcggggcc tggccgacct ccgctacgag gtcatgtaca
     6481 tcctcgaggc gcccgacgcc gccatccccg ccttcaagga cgtgtgggcc ggcatcggcg
     6541 actccatcgt ggtggtgggc ggcgacggcc tcttcaactg ccacatccac accgatgaca
     6601 tcggccccgc catcgaggcc gccatcgaga tcggcaagcc gtcgaagatc cgggtgaccg
     6661 acctcttgga ggaggtcgag gaggagcgct gggtccggga ggccgaggag caccccgagc
     6721 ccgagcctga cgccgccccg gtcgccaccg cggtggtggc catcgccacc ggcgacggcg
     6781 tgaagcggat cttccactcg ctcggtgccc gggtcctcac cggcggccag tcgatgaacc
     6841 cgtccacggc acagatcctg gaggcggtcg aggcggcgcc gtcggactgc gtggtgatcc
     6901 tccccaacaa caagaacatc atcgccgtgg ccgagcaggt cgacgccacc accgacaaga
     6961 cggtcgtggt cgtccccacc aaggggatcc ccgagggctt cgccgccctg gtggcctacg
     7021 accccgacgc cgagatcgcc gagaacgcgt cgggcatggc ggacgcggcg gccaacgtgc
     7081 tcgccggcga ggtcacccag gccgtgcggg actcgtcgtg cgacgtcggg ccgatcgtcg
     7141 agggcgactt cctcggcatc gcccgcgacg ggatccaggc ggtcgcgccg tcggtcagcg
     7201 aggcgaccat cggcctgctc gaccacctcc tgaccgagga ccacgagatc gtcaccctca
     7261 tcacgggcga ggggtcgtcc gacgccgaga cccgcaaggt cctgctctgg atcgaagagc
     7321 accacgacgg ggtggagacg gagacccacc acggcgggca gccgctctac ccctacttcg
     7381 tcggcatcga gtagcccgtg gtcgtggcgg acgacgaggg cgacgaggtc gtgacgccga
     7441 tcacgggcga gggccaccgg aacgaggcgg acgggccggc cggccggccc gtccgccggc
     7501 cgcgccaggt cacgtcgctc gccgggttcg acgatccgcc gccgccgctg cacgcgctcg
     7561 tccggtcctg gccgtgggcg ggctggctgg tggccgccgt ggtcgcgctg cggctcgtcg
     7621 ccgtggctgg catgcggctg tacctctacg tcgactcggc ggagtacgac cggatcgact
     7681 tcagcggcga ttggcggagg ccgtgggcca cgccgtgcct ctactggctg atccccggcc
     7741 gcgcccgcgg gctcgtcgtc ggccaggccc tcgtcggcgc ggtgtgctgg gccgccttgg
     7801 cgctcagcgc cgcggcctgg ttccggttcc gcacctcgcg gatcgtggtc gccgccgtgc
     7861 tcgcggggct gggctgcacc accatcgtca ccaactggga tgccgccaag ctctccgagt
     7921 cgctcggctt gtcgctgacg gtgctcgtca tcgcctcctg gctgaacctc atacgacgga
     7981 ccgaggtggg gacggcgctg ttcgtcgcgc tggcgaccct gccgtggctg ttcgtgcgcc
     8041 agagcctgat cccgacggcc tggctcgtgg tgggcctcgc ggcgctcgtc gctgcgatca
     8101 cctggcggcg gcgcggctcg gaaggcctcg cggtgcgggt cctcgccgcc ctgtgcctcg
     8161 tgctggcggc cgagaccgcg gtcgcgacgg tcacctacag ccacaaccag gagatcgtgc
     8221 aggagaacct gcgcgtcatc atctcgaacc gggtggcctc ggatccggcc cggctcgtgt
     8281 ggttcaccga ccacgggatg ccggcgccgg cctcgggccg gttcgacccg gagacgctgc
     8341 gctcggaccc ggcgttctcg acctgggtcg cgcacgaggg cccgagcacc tacctgcggt
     8401 acctcctgac ccacccctgg tacacggcga ccgccccgct cgacgacttc accgacgtcc
     8461 agcgctccac ggccgacgcc gtggagcccg cggcggcgat gctctcgccg ccggaccgct
     8521 acgccgccag ccgcccggtg atcccgtccg tcgtcgagga cgtcatcttc ggcgccggcg
     8581 acacgggcac ggtgatcttc ctcctggtcc tggtgggcgg ctggacgctg gcgcggatcc
     8641 gccggcgctc ggcgcgctgg gcgatcccct gggcgctggt cgggctggcg atcacgtcgc
     8701 tctacaccgg ctggcacgga gcgacccccg agctcggccg gctggcgctc ctgggcgcgg
     8761 tgacgctgcg gatcgggctg tacctgcagc tcgcggtgct catcgaagac gaggtcgtcg
     8821 gccgccgccg accggtcgtc
//
LOCUS       DATMFS010000284         6357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_264508, whole genome shotgun sequence.
ACCESSION   DATMFS010000284 DATMFS010000000
VERSION     DATMFS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264508"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VNQ33_05240"
     CDS             complement(<1..1232)
                     /locus_tag="VNQ33_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97542.1"
                     /translation="MGITDRIDLARGRGLTLGTLLEMVASVHPRRTLVTEPDGTSLTA
                     TGAAELVDLWAGGVAARSEPGDRVVIATPNGYEQFLLTLAVARAGRLPAPVNAQMAPT
                     EVDHVVADSGATLVVRSSDDVASDDLFGPAVPADPEDVAALFYTSGTTGTPKGAELSH
                     AGLLGGLSMVAAVPTELHRDELVVALPVAHIFGFAIVVGAAVAAIPAYLLPRFNPVEV
                     LDAIESRRATVFAGVPAMYRMLEEAGADDRDLKSIRLWISGADAMPADLAKRFKRRGA
                     SATLPLIGSLGEAIFAEGYGMVETAGGVAIRVSPPMVPASIGGSVGMPLPGVRFKVVD
                     EDGAEVPLGGVGELWLCGPGVLKGYHGAPEASAGALTDDGWLRTGDLARRGPLGIINF
                     EGRMKDVIKRGGYSVYAV"
     gene            complement(1258..4590)
                     /locus_tag="VNQ33_05245"
     CDS             complement(1258..4590)
                     /locus_tag="VNQ33_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWJ97543.1"
                     /translation="MPATPSDAELLEGLNPVQREAVIHDEGPLLVIAGAGSGKTRVLT
                     SRIAHLIKNGVSPFEILAITFTNKAAQEMKHRVGALVGPVAEKMWVSTFHSACVRILR
                     RDGHRLGYPSSFTIYDQADAVRLTGYVIRDKGLDAKRFPPRSVHGTISAAKNDDVGPE
                     AFAERVREPFERKIADVYAEYQDRLLKAGAMDFDDILRNAVELFRREPEVLDHYRRRF
                     QHVLVDEYQDTNKVQNELILQLAGEHHNVCIVGDSDQCLLPGTLVDTPDGQRPIEDIQ
                     VGDHVLGARGGDVAEAGTVTAVAPGRYEGRVYRVKAGDGEIVGTPHHMVPVRVVLDEG
                     THIVYLMGRTGQGYRIGRTKSVRPGNKGDQQLGTLVRMNQERGDCLWILGVYPTLEDS
                     AWWESWFAAQYGLPTACFHTVGRSGMAMGQEALDRLFAAIDTETRAKDLLEDFDLHFE
                     FPHVVPRNGRRRQTLNLTMFSDVRGSNAGHRIQWSSNRGDIADRLREAGFPARDGKRP
                     GTFLLELYRSSFVEALETANAIAFAGGLDVRRRASVAGHIWDLQPLSHLRPGMEMLHA
                     SEDGTLSPVRVDSVSSGDYSGPVHDLEVSPTHTFVAGSLVVRNSIYRFRGADIRNILE
                     FEEAFPDATVVVLEQNYRSTQTILDAANAVITNNLSRKPKELWTDEGDGHAIVRYHAD
                     TETDEAQWVAHEIAKLHDSGDHTWGDVAVFYRTNGQSRVMEEHLLRAGIPYKVVGGTR
                     FYDRREIKDALAYLKAVVNPADEVAVKRVLNVPKRGIGDSSVNKLEAWAAMHGYTFMQ
                     ALRRAGEAGIQGRAVKGIADFLSVIDASAEVVDQGPGPLLESVLNRSGYLDELQVEHS
                     VDAEGRLENLSELVGSAREAESVDVFLEQVSLVADSDQIPDDDSQVVLMTLHSAKGLE
                     FPAVFLIGLEDGVFPHLRSLGEPTELEEERRLAYVGITRARERLYLTHAWSRTIFGST
                     QYNPPSRFLDEIPQRLVQSIDGNRRTSRAGSWSDSAAMDSSTSSWGDRPERRSRISSE
                     QRQRNRERMVDQAIAAGQQAASSASTVSGYKVGDDVVHGKWGEGVVLDLQGSGDKTEI
                     TIHFPTVGQKVLLLAWAPLKKA"
     gene            4668..5996
                     /locus_tag="VNQ33_05250"
     CDS             4668..5996
                     /locus_tag="VNQ33_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWJ97544.1"
                     /translation="MALISSDVPASSAADSRSAKRTSRRKQSVQSRAVKRTLLLVVTL
                     FVFYYVVIPTFARSKESADRLAQLNPLWILLAVGLEIASLLCYTVLTRVTLPPEPRLS
                     LFTIFRIQLATKAVGNLVPGGSAAGGTLGYSLLTTAGVAPTAAGFTLATVGLGSAVVL
                     NLILWITLLISIPMNGFRPAYVTAAIVGVLLLAFAAGLVYLLIEGRDVAERVLRAIFR
                     KLPYVEEETAARFVHQTADRIQDLARQPELVRRGVLWATANWLLDAACLWTLLHAFGY
                     TMNPINLVVAYGVIGVVASIPITPGGLGVVETALPALLVTFGAPPVPAGFAVLSWRLI
                     QFWMPIPIGGLSYASLKLGPLGRRRRLAAVRDLAREAGENARVRVWDQQTGEYRVVKA
                     ETAEALLAGDDADRLSLEPPPDHPVPGDLDSEDLDAEDLDDGPQAAAGPI"
     gene            complement(5997..>6357)
                     /locus_tag="VNQ33_05255"
     CDS             complement(5997..>6357)
                     /locus_tag="VNQ33_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97545.1"
                     /translation="GASKRDVNLSPEDLAAVKARCEDEEDLCVLGLRFSGDFMAPAER
                     FARLEQELGESFVGVTIDSSKGNVHGIRPMAHSVLTEDLVDEPGHPTQDALHQVLDLF
                     RTRLVPPGREVPHRRRA"
BASE COUNT          920 a         2259 c         2176 g         1002 t
ORIGIN      
        1 tcgaccgcat agaccgagta gccgccccgc ttgatgacgt ctttcatccg gccctcgaag
       61 ttgatgatgc ccagcggccc acggcgggcc aggtcgccgg tgcggagcca gccgtcatcg
      121 gtcagggcgc cggcgctcgc ctccggagca ccgtggtacc ccttcagcac gccgggaccg
      181 cagagccaca gctcgccgac gccgcccagg ggcacctcgg caccgtcctc gtcgacgacc
      241 ttgaaccgca cgccgggcag cggcatcccg accgagccgc cgatcgacgc cggcaccatc
      301 ggcggcgaca cccggatggc caccccgccc gccgtctcga ccatgccgta gccctcggcg
      361 aagatcgcct caccgagcga cccgatcagc gggagggtgg ccgacgcgcc ccggcgcttg
      421 aaccgcttgg cgagatcggc cggcatggcg tccgcgcccg agatccacag ccggatcgac
      481 ttcaggtccc ggtcgtcggc gcccgcctcc tcgagcatcc ggtacatggc cggcaccccg
      541 gcgaacaccg tcgcccgacg cgactcgatg gcgtccagca cctcgaccgg gttgaaccgg
      601 ggcaggaggt acgccgggat ggcagccacc gctgcgccca cgacgatggc gaacccgaag
      661 atgtgcgcca ccggcagggc gaccaccagc tcgtcacggt gcagctcggt gggcaccgcc
      721 gccaccatcg acaggccgcc gagcaggccg gcgtggctga gctcggcgcc cttcggcgtg
      781 cccgtggtgc ccgaggtgta gaagagggcg gcgacgtctt cgggatcggc gggcacggcc
      841 ggaccgaaca gatcgtcgct cgccacgtcg tcgctcgatc ggacgacgag cgtcgccccc
      901 gagtcggcga cgacgtggtc cacctccgtc ggcgccatct gggcgttcac cggcgcgggc
      961 agccggccgg cccgggcgac ggcgagggtc aggaggaact gctcgtagcc gttcggcgtg
     1021 gcgatgacga cccggtcgcc cggctcgctg cgagcggcga ccccgccggc ccagaggtcg
     1081 accagctcgg cggcgccggt cgcggtcagg ctcgtgccgt ccggctcggt caccagggtg
     1141 cgtcgggggt gcacgctcgc caccatctcc agcagcgtgc ccagggtcag ccccctgccc
     1201 cgcgccaggt cgatgcggtc ggtgatcccc atgaggtcca ccctagggcc gcgctgatca
     1261 ggccttcttc agcggcgccc acgccaagag cagcaccttc tggcccaccg tcgggaagtg
     1321 gatggtgatc tcggtcttgt ccccagaccc ctggaggtcg agcaccacgc cctcgcccca
     1381 cttgccgtgg acgacgtcgt cgccgacctt gtacccgctg acggtcgagg ccgacgacgc
     1441 ggcctgctgc cccgctgcga tggcctggtc gaccatccgc tcgcggttgc gctggcgctg
     1501 ctcggacgag atccggctcc ggcgctcggg ccgatcaccc cagctcgagg tcgaggaatc
     1561 catcgccgcg ctgtccgacc agctgccagc acgcgaggtg cggcggttgc cgtcgatgga
     1621 ctgcaccagg cgctggggga tctcgtcgag gaaccggctc ggcgggttgt actgcgtcga
     1681 gccgaagatc gtccggctcc acgcgtgcgt caggtagagc cgctcgcggg cccgggtgat
     1741 gccgacgtag gcgaggcgcc gctcctcctc cagctcggtg ggctccccca gcgagcggag
     1801 gtgcgggaag accccgtcct cgagcccgat gaggaacacg gccggaaact ccaggccctt
     1861 ggccgagtgc agggtcatga gcacgacctg cgagtcgtcg tcggggatct ggtccgagtc
     1921 ggcgaccagg ctgacctgct cgaggaacac gtcgaccgac tccgcttcgc gtgcggagcc
     1981 caccagttcg gacaggttct ccagccgtcc ctcggcatcg accgagtgct ccacctgcag
     2041 ctcgtcgagg tagcccgacc ggttgagcac cgactcgagc agcgggcccg gcccctggtc
     2101 cacgacctcg gccgatgcat cgatcaccga caagaagtcg gcgatgccct tgaccgcccg
     2161 gccctggatc ccggcttcgc cagcccgccg cagcgcttgc atgaacgtgt agccgtgcat
     2221 ggccgcccag gcctcgagct tgttcaccga cgagtccccg atgccccgct tcggcacgtt
     2281 gagcacccgc ttgaccgcca cctcgtccgc cgggttcacc acggctttca ggtaggccag
     2341 ggcgtctttg atctcccgcc ggtcgtagaa gcgggtaccg cccacgacct tgtacgggat
     2401 gccggcgcgc agcaggtgct cctccatgac ccggctctgc ccgttggtcc ggtagaagac
     2461 ggcgacgtcg ccccacgtgt ggtcgcccga gtcgtgcagc ttggcgatct cgtgcgccac
     2521 ccactgcgcc tcgtcggtct cggtgtcggc gtggtagcgg acgatggcgt gcccgtcgcc
     2581 ctcgtcggtc cacagctcct tgggcttgcg gctcaggttg ttggtgatga ccgcgttggc
     2641 cgcatcgagg atcgtctgcg tcgatcggta gttctgctcc agcaccacga cggtggcatc
     2701 cggaaacgcc tcctcgaact ccaggatgtt ccgaatgtcc gctcctcgaa agcggtaaat
     2761 tgagttcctc acgaccaagg atccggcaac gaaggtgtgg gttggcgaca cctcgaggtc
     2821 gtgcaccggg ccggagtagt cgcctgagga cacggagtcc acgcgcacag gcgagagcgt
     2881 gccgtcctcg ctggcgtgca gcatctccat gcctgggcgc aggtggctga gaggttgcag
     2941 gtcccagatg tgcccagcga cgcttgcgcg acgtcgcaca tcaaggccac ctgcaaaggc
     3001 aatggcgttg gctgtctcga gcgcctcgac aaacgacgat cggtacagct cgagcaggaa
     3061 ggtcccgggc ctcttgccat cgcgggccgg gaaacccgct tcgcggagac gatcggcaat
     3121 gtcgccccgg ttggaggacc actggatccg gtgtccggcg ttggagccac ggacgtcgga
     3181 gaacatcgtg aggttcaagg tctgtctgcg acgtccattc ctcggcacga catgggggaa
     3241 ctcgaagtga aggtcgaagt cttcgagcag gtcctttgct cgtgtctccg tgtcaatcgc
     3301 agcaaacagc cgatcgaggg cctcctgccc catagccatt cccgaccggc cgacggtgtg
     3361 gaagcacgca gtgggtaggc cgtattgagc agcaaaccag gactcccacc atgctgagtc
     3421 ctccaaggtc gggtacacgc caagaatcca caggcagtcg ccgcgctcct ggttcatgcg
     3481 aaccagtgtc ccgagctgct ggtcgccctt gttccccggt cgcacgctct tcgtccgccc
     3541 aatccggtag ccctgaccgg tgcgtcccat caggtagacg atgtgggtgc cctcatccag
     3601 cacaacccgt accgggacca tgtggtgcgg ggttccgacg atctcgccat cacccgcctt
     3661 cacccggtag acgcggccct cgtagcgacc aggagcgacg gcggtgacgg tgccagcctc
     3721 ggcgacgtca ccgccacggg cgcccaggac gtggtcgccg acctggatgt cctcgatcgg
     3781 gcgctggccg tcgggggtgt cgaccagcgt gccggggagg aggcactggt cgctgtcgcc
     3841 gacgatgcag acgttgtggt gctctccggc gagctggagg atcagttcgt tctggacctt
     3901 gttggtgtcc tggtactcgt cgacgaggac gtgctggaag cggcggcggt agtggtcgag
     3961 cacctcgggc tcgcggcgga acagctcgac ggcgttgcgg aggatgtcgt cgaagtccat
     4021 ggcaccggcc ttgagcaggc ggtcctggta ctcggcgtag acgtcggcga tcttgcgctc
     4081 gaacggctcc cgcacccgct cggcgaacgc ctcggggccg acgtcgtcgt tcttggccgc
     4141 cgagatggtg ccgtgcaccg agcggggcgg gaagcgcttg gcgtcgagcc ccttgtcacg
     4201 gatgacgtag ccggtgaggc ggacggcatc agcctggtcg tagatcgtga acgaggacgg
     4261 gtagccgagg cggtgcccgt cgcgccggag gatgcgcacg caggccgagt ggaacgtcga
     4321 cacccacatc ttctcggcca ccggtccgac cagggcgccg acgcggtgct tcatctcctg
     4381 ggccgccttg ttggtgaagg tgatggccag gatctcgaac ggcgacacgc cgttcttgat
     4441 gaggtgggcg atgcgcgagg tgagcacccg ggtcttgccc gaaccggcgc cggcgatgac
     4501 gagcaggggt ccctcgtcgt ggatcacggc ctcccgctgc acggggttca gcccctcgag
     4561 gagctcggcg tcagacggcg tggcaggcat cgcggccacc ctaccggcgg cccctgacag
     4621 cggaatccgg gccgcgatgg cggagaaggg ggtaccggac gggaagaatg gccctcatct
     4681 cctctgacgt gccggcgtcc agcgccgccg attcccgtag cgccaagcgg acctccaggc
     4741 gcaagcagtc ggtccagagc cgcgcggtca agcgcacgct gctgctggtc gtcacactgt
     4801 tcgtcttcta ctacgtggtc atcccgacct tcgcccggtc gaaggagtcg gcggaccgcc
     4861 tggcccagct caacccgctg tggatcctgc tggcggtggg cctcgagatc gcctccctgc
     4921 tctgctacac ggtgctcacc cgggtgaccc tgccgccgga gccccggctg tcgctgttca
     4981 ccatcttccg gatccagctg gcgaccaagg ccgtcggcaa cctggtgccg ggcgggtcgg
     5041 cggccggcgg cacgctgggc tacagcctcc tcacgacggc cggggtcgct cctaccgctg
     5101 cggggttcac gctcgccacg gtcggtctgg gttcggccgt ggtgctcaac ctcatcttgt
     5161 ggatcaccct gctcatctcg atcccgatga acggcttccg cccggcctac gtcaccgccg
     5221 ccatcgtcgg cgtgctgctc ctcgccttcg cggcgggcct cgtctacctg ctgatcgagg
     5281 gccgcgacgt ggccgagcgg gtcctgcggg ccatcttccg gaagctcccg tacgtggagg
     5341 aggagacggc ggcccgcttc gtccaccaga ccgccgatcg catccaggac ctggcccggc
     5401 agcccgagct ggtccgccgg ggcgtgctgt gggcgacggc caactggctc ctcgatgccg
     5461 cgtgcctgtg gaccctgctg cacgcgttcg gctacacgat gaacccgatc aacctcgtgg
     5521 tcgcctacgg cgtgatcggc gtcgtggcgt cgatccccat cacccccggt ggcctcggtg
     5581 tggtcgagac cgcgctgccg gccctgctgg tcaccttcgg cgccccgccc gtgccagccg
     5641 gcttcgccgt gctgtcgtgg cgcctgatcc agttctggat gccgatcccg atcggcggcc
     5701 tgtcctacgc ctcgctcaag ctcggtccgc tcggccggcg gcggcggctc gccgccgtgc
     5761 gcgacctggc gcgagaggcc ggcgagaacg cccgcgtccg tgtctgggac cagcagaccg
     5821 gcgagtaccg cgtggtcaag gccgagaccg ccgaagccct gctggccggc gacgatgccg
     5881 atcgcctgtc gctcgagccc ccgccggacc atccggtccc cggcgacctc gacagcgagg
     5941 acctcgacgc cgaagacctc gacgacggac cgcaggcggc ggccggcccg atctgatcaa
     6001 gcgcgccggc ggtggggcac ctctcgcccc ggcggcacca gacgggtgcg gaacaggtcc
     6061 aggacctggt gcagcgcgtc ctgcgtgggg tggccgggct cgtccacgag gtcctcggtg
     6121 agcaccgagt gggccatcgg gcggatgccg tggacgttgc ccttcgacga gtcgatcgtg
     6181 acgcccacga acgactcgcc cagctcctgc tcgagccggg cgaagcgctc ggccggcgcc
     6241 atgaagtcgc cgctgaagcg caggccgagg acgcacaggt cctcctcgtc ctcgcaccgg
     6301 gccttgaccg cggcgaggtc ctcgggcgac aggttcacgt cgcgcttgga ggcgccg
//
LOCUS       DATMFS010000285         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80017, whole genome shotgun sequence.
ACCESSION   DATMFS010000285 DATMFS010000000
VERSION     DATMFS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80017"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2455
                     /gene="ligA"
                     /locus_tag="VNQ33_05260"
     CDS             <1..2455
                     /gene="ligA"
                     /locus_tag="VNQ33_05260"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWJ97546.1"
                     /translation="DEQAVDRAAALRTEIAEHNRRYHTDDAPTISDADYDELVRELRA
                     IEERFPELAVADSPTSQVGAPPSATFAPVVHRQPMMSLDNAFSADELVAWGERLARRL
                     ARTAKAAEIAAGRAEPATLFDEPGDAPAEDGGAGVDPADVDPADVDPDVSAPDLADVA
                     PDVAEAVGYCCELKIDGVAISLRYEDGILVQAATRGDGKVGEDVTANVRTIDDIPDRL
                     NDTPAGIPPVLEVRGEVYMSFEAFGDLVAFQEEENRVRAEAGRKPAPVASNPRNAAAG
                     SLRQKNAEVTARRKLSMWSYQLGEVAGGPEFATHQETLDQLDAWGFAVNPEHRVLDTA
                     DEVAAFCDHWQHHRHDLPYEIDGVVVKVDDLARREALGFTARAPRWAIAYKFPPEERI
                     TVLERIDVSVGRTGRVTPFAVLDPVFVGGVTVARATLHNQDQVAAKDVREGDAVIVRR
                     AGDVIPEVVGPYLPDAKAEKAHEARPPWEFPKFCPSSRPVELVRPEGESDTRCPDPEC
                     PFKVAGMIEHFAGRGSMDIEGFGVQRIQLFLDLGLIGDVADIFSIDWDRLAAIRAEIT
                     GWAGEALAAARERTQRPKASWDEVVLPEDVVAAQPEQLSADVPAHLVASSTEDPQQLK
                     AIAGTLAGLGDDGVANLKAGIEAARDRPLANLLVGLNIRHLGPSGSLALARAFGHLDA
                     IVEAPVEAMAAVDGVGTVIAESVQAWLAVEVHRDLVARLRGEVNVEGPEVSTLPQVLL
                     GKNVVVTGTLEGYTRDSAEAAITDRGGKSPGSVSKKTTALVIGESPGASKVTKAESLG
                     IPVLDLAGFEHLLETGELP"
     gene            complement(2489..>2901)
                     /locus_tag="VNQ33_05265"
     CDS             complement(2489..>2901)
                     /locus_tag="VNQ33_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97547.1"
                     /translation="FAAAAWLIGSTDSNQNADLNKAVIISLTPNEGAQAVRQTEVGAD
                     LAVGYDGRLTINGIEIPEDQMVGARDPKDVSPEDLAQNGLRPNNRNQVYFKPGPGKVI
                     EKFEPGTIDITLRYFKDGQPNEGAGTVQWSIKAI"
BASE COUNT          407 a         1048 c         1047 g          399 t
ORIGIN      
        1 cgacgagcag gcagtcgacc gggccgcggc cctccggacg gagatcgcgg agcacaaccg
       61 gcgctaccac accgacgatg cgcccacgat ctcggatgcg gactacgacg agctggtccg
      121 ggagctgcgg gcgatcgagg agcgcttccc ggagctggcg gtcgccgatt cgcccaccag
      181 ccaggtcggc gcgcccccgt cggccacgtt cgcccccgtg gtgcaccgcc agccgatgat
      241 gtcgctcgac aacgccttct cggccgacga gctggtggcc tggggcgagc ggttggcccg
      301 ccgactggcc cgcaccgcca aggcggcgga gatcgcggcc ggccgggccg agccggcgac
      361 cctgttcgac gagccgggcg atgcccctgc ggaggacggc ggggcggggg tcgaccctgc
      421 cgacgtcgac cctgccgacg tcgaccccga cgtctccgcc ccggacctgg ctgacgtggc
      481 gcccgatgtg gcggaggcgg tcggctactg ctgcgagctc aagatcgacg gggtcgccat
      541 ctcgctgcgc tacgaggacg gcatcctcgt ccaggccgcc acccgcggcg acggcaaggt
      601 gggggaggac gtcaccgcca acgtccggac catcgacgac atcccggatc ggctcaacga
      661 cacgccggcg ggcatcccgc ccgtgctcga ggtgcgcggc gaggtgtaca tgtcgttcga
      721 ggccttcggt gacctggtcg ccttccagga ggaggagaac cgggtccgcg ccgaagccgg
      781 ccgcaagccg gcaccggtgg cgtccaaccc caggaacgcc gcggccgggt cgctgcgcca
      841 gaagaacgcc gaggtcacgg cccgccgcaa gctctccatg tggagctacc agctgggcga
      901 ggtggccggc ggccccgagt tcgccaccca ccaggagacg ctcgaccagc tcgacgcctg
      961 gggcttcgcc gtcaacccgg agcaccgggt gctggacacg gccgacgagg tggcggcgtt
     1021 ctgcgaccac tggcagcacc accgccacga cctcccgtac gagatcgacg gcgtcgtcgt
     1081 gaaggtcgac gacctcgcac ggcgggaggc gctcggcttc acggcccgtg cgccccggtg
     1141 ggccatcgcc tacaagttcc cgcccgagga gcgcatcacc gtgctcgagc ggatcgacgt
     1201 ctcggtgggc cgcaccggtc gcgtcacgcc cttcgccgtg ctcgacccgg tgttcgtcgg
     1261 cggcgtcacc gtcgcccggg ccacgctcca caaccaggac caggtggccg ccaaggacgt
     1321 gcgcgagggg gacgcggtca tcgtgcgccg ggccggcgac gtgatccccg aggtcgtggg
     1381 cccgtacctg cccgacgcca aggccgagaa ggcgcacgag gcccggcctc cgtgggagtt
     1441 ccccaagttc tgcccgtcgt cgcgccccgt cgagctcgtc cggccggagg gggagagcga
     1501 cacccgctgc cccgacccgg agtgcccgtt caaggtggcc gggatgatcg agcacttcgc
     1561 gggccggggg tcgatggaca tcgagggctt cggcgtccag cgcatccagc tcttcttgga
     1621 cctgggcctc atcggcgacg tggcggacat cttctccatc gactgggacc gcctggccgc
     1681 catccgagcc gagatcacgg gctgggcggg tgaggcgctc gccgcggccc gcgagcggac
     1741 gcagcggccg aaggcgtcgt gggacgaggt cgtgctgccc gaggacgtgg tggcggccca
     1801 gcccgagcag ctgtcggctg acgtgcccgc ccacctcgtg gcgtcgagca ccgaggaccc
     1861 ccagcagctc aaggccatcg ccggcaccct cgccggcctg ggcgacgacg gcgtcgccaa
     1921 cctgaaggcc ggcatcgagg cggcgcgcga ccggccgctg gccaacctgc tggtgggtct
     1981 caacatccgc cacctgggcc cgtcgggctc gctcgccctg gcccgggcct tcggccacct
     2041 cgacgccatc gtcgaagcac cggtggaggc catggccgcc gtcgacggcg tcggcacggt
     2101 catcgccgag tcggtgcagg cctggctggc ggtcgaggtg caccgggacc tcgttgcccg
     2161 cctgcgcggc gaggtcaacg tcgaagggcc cgaggtgtcg acgctgccgc aggtgctgct
     2221 gggcaagaac gtcgtggtca cgggcacgct cgagggctac acccgagaca gcgccgaggc
     2281 cgccatcacc gaccggggcg gcaagtcgcc gggctcggtg tcgaagaaga ccaccgccct
     2341 cgtcatcggc gagtcgcccg gcgcgtcgaa ggtcaccaaa gccgagtccc tcgggatccc
     2401 ggtgctcgac ctcgccggct tcgagcacct gctggagacg ggcgagctcc cctgatcccc
     2461 cggcggccgg taccgccgga tcagccggtc agatcgcctt gatcgaccac tggacggtgc
     2521 cggcgccctc gttgggctgg ccgtctttga agtaccgcag cgtgatgtcg atggtccccg
     2581 gctcgaactt ctcgatgacc ttgccgggac cgggcttgaa gtacacctgg ttgcggttgt
     2641 tgggccgaag cccgttctgg gccaggtcct caggcgagac gtccttcgga tcgcgggcac
     2701 ccaccatctg gtcctcgggg atctcgatcc cgttgatggt cagccgcccg tcgtagccga
     2761 cggccaggtc ggcgccgacc tcggtctggc gcaccgcctg ggccccttcg ttgggcgtga
     2821 ggctgatgat gacggccttg ttgaggtcgg cgttctggtt cgagtcggtg gatccgatca
     2881 gccaggccgc cgcggcgaag g
//
LOCUS       DATMFS010000286         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_33841, whole genome shotgun sequence.
ACCESSION   DATMFS010000286 DATMFS010000000
VERSION     DATMFS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33841"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VNQ33_05270"
     CDS             <1..1260
                     /locus_tag="VNQ33_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ97548.1"
                     /translation="IVGGAAHLLDCIPSKAMIATGGAMNDMRRASGMGLVHVDAELDF
                     EALRARISGIENRLEGSVTGMLRSQGVNLLQGTGRLLDAHHVEVTEADGTTRVVEADT
                     VVLSTGSRPRIPEWAPIDGRRVLTTRDAYPPPELPSHLVVVGSGVTGVEFVHMFSAFG
                     ADVTLIVSRQQVLPQKDPEVAAVLEDDFLGRGVRLFKGARAEGIDVTDDDVAVRCDDG
                     RVARGSHALLCIGSIPNSEGLGLEDIGVALDHGYVTVDHNCKSSVPNIYAAGDLSGKL
                     PLSSVASMHGRKIAEHALGLHAGPHRHIDYEKAASAIFTDPEIADVGLAEADAFADGR
                     KVRVTKVPFSASAKALINDDPRGFVKIISDPATGVVLGGSIVGQHAAELISVIAVAVT
                     AGLKVSDLAESLFVHPALAEALSDAAD"
     gene            1271..>2265
                     /locus_tag="VNQ33_05275"
     CDS             1271..>2265
                     /locus_tag="VNQ33_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97549.1"
                     /translation="MTAPVDHAGPEPGAVDHTLSDARTRPDLGGRAELPPVHRLRGLT
                     ARGRAAAVALGVLLALVPLIAAAVNHGRWEPQGDDALIELRARDVFTDRNPLVGQPST
                     SGSYGEKAENVAHPGPLGFVVLAPSTRVLGPVAGTLVAAAAVSAASMLAVAWLLFRQL
                     GPRGGAAGAALAALAAFSAGAAGLVDPLSSNFGRMALLAASVGVWALLCGDLRTAPLT
                     AAFWTFAAQQHLSVLPAAAVLGGVGVLAAAVWVGRLRGPGRRQALAWAGAALAVGLVL
                     WAPVLYQEVTGDPGNLTALSHYSGDGQREDLGPRSALNQLANALGPRPFVGRSSP"
BASE COUNT          275 a          875 c          797 g          318 t
ORIGIN      
        1 atcgtgggcg gtgccgccca tctgctcgac tgcatcccct cgaaggcgat gatcgccacc
       61 ggcggggcca tgaacgacat gcgccgggcc agtggcatgg gtctggtcca cgtcgacgcc
      121 gaactggact tcgaggcgct gcgagctcgc atctccggca tcgagaaccg cctcgagggg
      181 tcggtcaccg ggatgctccg gagccagggc gtgaacctgc tgcagggcac gggccggctc
      241 ctcgacgccc accacgtcga ggtgaccgag gccgacggca ccacccgggt cgtcgaagcc
      301 gacaccgtgg tgctgtccac cggcagccgg ccccgcatcc ccgagtgggc gcccatcgac
      361 ggccgccgcg tcctcaccac ccgcgacgcc tacccgccgc cggagttgcc gtcgcacctc
      421 gtggtggtcg ggtccggtgt caccggtgtg gagttcgtcc acatgttctc ggcgttcggc
      481 gctgacgtca cgctcatcgt gagccgccag caggtcctcc cgcagaagga ccccgaggtc
      541 gccgccgtgc tcgaggatga cttcctcggc cgcggcgtcc gcctgttcaa gggggctcgg
      601 gccgagggca tcgacgtcac cgacgacgac gtcgcggtgc gctgcgacga cggccgcgtc
      661 gctcggggat cgcatgcgct gctgtgcatc gggtcgatcc cgaactccga ggggctcggc
      721 ctggaggaca tcggcgtggc gctcgaccac gggtacgtca cggtggacca caactgcaag
      781 agctcggtgc ccaacatcta cgccgccggc gacctgtcgg ggaagctccc gctctcgtcg
      841 gtggcgtcga tgcatgggcg caagatcgcc gagcacgcgc tcggcctgca cgccgggccg
      901 caccgccaca tcgactacga gaaggcggcg tcggccatct tcaccgaccc cgagatcgcc
      961 gacgtcggac tcgccgaggc cgatgcgttc gccgacggcc gcaaggtgcg ggtgaccaag
     1021 gtcccgttct cggcatcggc caaggccctc atcaacgacg acccccgcgg cttcgtgaag
     1081 atcatctccg atccggccac cggcgtcgtc ttgggcgggt ccatcgtcgg ccagcacgcc
     1141 gccgagctca tctcggtgat cgccgtcgcc gtcaccgccg ggctcaaggt cagcgacctg
     1201 gccgagagcc tgttcgtgca cccggccctg gccgaggccc tgagcgacgc tgccgactga
     1261 acgacccgcc gtgaccgcac ccgtcgatca cgccggaccc gagcccggcg ccgtggacca
     1321 caccctctcg gacgcccgca cccgtccgga cctcgggggc cgggccgagc tgccgcccgt
     1381 ccaccgcctg cgcggcctga cggcgcgggg ccgggcggcc gcggtcgccc tgggcgtcct
     1441 cctggcgctc gtgccgctga tcgccgccgc tgtgaaccac gggcggtggg agccgcaggg
     1501 cgacgacgcc ctcatcgagc tgcgggcgcg cgacgtcttc accgaccgca acccactcgt
     1561 cggccagccc tccaccagcg ggtcctacgg cgagaaggcg gagaacgtcg cccatcccgg
     1621 accgctcggc ttcgtggtcc tggcgcccag cacccgggtg ttgggcccgg tcgccggcac
     1681 gctcgtggcg gccgccgcgg tgagcgcggc gtcgatgctg gccgtcgcct ggctgctgtt
     1741 ccgccagctc ggcccgaggg gcggcgcggc cggcgccgcg ctggccgccc tcgcggcctt
     1801 ctccgccggc gcggccggcc tggtcgatcc gctgagctcg aacttcggcc gcatggccct
     1861 gctcgccgcg tctgtggggg tgtgggcact gctgtgcggc gacctgcgca cggcgcccct
     1921 caccgccgcc ttctggacct tcgccgccca gcagcacctg tccgtcctgc cggccgcggc
     1981 cgtgctgggc ggcgtcggcg tgctcgctgc ggcggtgtgg gtcgggcggc tccgcggtcc
     2041 cggccgccgc caggcgctgg cgtgggccgg cgccgcgctc gccgtcgggc tggtcctgtg
     2101 ggcaccggtg ctctaccagg aggtcacggg cgaccccggc aacctgaccg ccctgtcgca
     2161 ctactcgggc gacggccagc gcgaggacct cgggccgcgc tcagcgctca accagctggc
     2221 caacgcgctg ggccctcgac cgttcgtcgg gcgatcgagc cccaa
//
LOCUS       DATMFS010000287         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_289942, whole genome shotgun sequence.
ACCESSION   DATMFS010000287 DATMFS010000000
VERSION     DATMFS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289942"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VNQ33_05280"
     CDS             complement(<1..626)
                     /locus_tag="VNQ33_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97550.1"
                     /translation="MMKSRGAAVIGAFALIALAIFIRGLLVSDDGGGSDGPKQSSSGG
                     APVVACTPELLAVCEAMAADGLIADDPPALDLPDAAAPPSDVDAWVTWNPAPQIANYV
                     SSRSGTTSVWSTTEAFGSAPEVILADGSTATNLAADCRATTTWACLGGLAPELSIGVG
                     DPATSEGIARLAPFAQAFAADDDPSTLDVGALDAIVRSPAEGQSDATT"
     gene            complement(676..2136)
                     /locus_tag="VNQ33_05285"
     CDS             complement(676..2136)
                     /locus_tag="VNQ33_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97551.1"
                     /translation="MLSEPKQMQRAVEAFVAEIAPAMRDVVARLPDLDSKEMDRDVAT
                     EAFHLTTALIDVDRSHTDDELWGLIFAFGRWLPAQLARAKPDDLRRSDLLVGRAGWLD
                     QVSPMFEILVTADRRFGTSHSRTYYDRSLNLAFTVAALEAAPSRAELQAIDEFRNQML
                     QAMSGLPSSPLPPRSGPPATGTPAAAGADQAAIAAGGTAAPAAEPLPPARPIEELLAE
                     LDDLVGLDSVKTEVRLVTNLLRVQQLRTERKLPTTPQSRHLVFTGNPGTGKTTVARLL
                     AAIYRSLGVVERGQLVETDRSGLVSGFVGQTALKVMEVADRATGGVLLIDEAYALARG
                     GENDFGREAIDTIVKVIEDRREELVVIAAGYPDEMAEFIGANPGLASRFPKTIFFPDY
                     DDDELWRIFVDQGEEAGYQADAGAEAKVRAWFASVPRDKGFGNGRLARNLFEDAVARQ
                     ASRVVALTDPTDEQLTALTAADIAEPGQGPRHSSTT"
     gene            complement(2144..>2748)
                     /locus_tag="VNQ33_05290"
     CDS             complement(2144..>2748)
                     /locus_tag="VNQ33_05290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97552.1"
                     /translation="ATVPVIRPATAADLPAVGRALAAAFDGDPVWSHLASPKADWSTR
                     AAAWFEAEARSQHRGHGEVLVDHQVRGAAIWAPPGHWRGTVSEGLALAIPSVRLFRGR
                     LRRAIADLAAMERKHPKDPPHWYLAILGTDPEHQGSGVGSALIRAVTDRCDEQGLGAY
                     LESSKEQNVPFYARHGFEVREEIAPGGGPPIWLMWRDPRG"
BASE COUNT          355 a          954 c         1057 g          382 t
ORIGIN      
        1 atcgtggtgg catccgactg accctctgcg gggctccgga cgatggcgtc gagcgcaccg
       61 acgtcgagcg tcgaggggtc gtcgtcagcg gcgaacgcct gggcgaacgg agcgaggcgg
      121 gcgatgccct cggacgtggc cggatcgccg acgccgatcg acagctccgg ggccagcccg
      181 ccgaggcagg cccaggtcgt ggtggcccgg caatcagcag cgaggttcgt cgccgtgctc
      241 ccgtcggcga ggatcacctc cggtgccgac ccgaacgcct cggtggtgga ccagaccgac
      301 gtggtgcccg agcgggagct gacgtagttg gcgatctgcg gtgccgggtt ccaggtgacc
      361 caggcgtcga cgtcggacgg aggcgctgcc gcgtcgggaa ggtcgagcgc tgggggatcg
      421 tcggcgatga ggccgtcggc cgccatcgcc tcgcagacgg cgaggagctc cggggtgcag
      481 gccacgaccg gtgcgccgcc gctgctcgac tgcttggggc cgtctgagcc gccgccgtcg
      541 tcgctcacga ggaggccgcg gatgaagatg gcgagcgcga tcagggcgaa cgccccgatg
      601 accgcagcgc cccggctctt catcatggtg ccgaacgcta ctggtgcacc gctcggcgcc
      661 ggtgccggag cgccgtcagg tggtgctgct gtggcggggc ccctggccgg gctcggcgat
      721 gtccgccgcg gtgagggcgg tgagctgctc gtcggtgggg tcggtcagcg ccacgacgcg
      781 gctggcctgg cgggcgaccg cgtcctcgaa caggttccgg gccaggcggc cgttgccgaa
      841 gcccttgtcc cgcgggaccg aggcgaacca cgcccggacc ttcgcctcgg cgccggcatc
      901 ggcctggtag ccggcctcct ctccctggtc gacgaagatc cgccacagct cgtcgtcgtc
      961 gtagtccggg aagaagatcg tcttcgggaa gcgggaggcc aggcccgggt tggcgccgat
     1021 gaactcggcc atctcgtcgg ggtacccggc cgcgatcacc accagctcct cgcgccggtc
     1081 ctcgatcacc ttcacgatcg tgtcgatggc ctcgcggccg aagtcgttct cgccgccgcg
     1141 ggccaaggcg taggcctcgt cgatgagcaa gacgccaccg gtcgcgcggt ccgcgacctc
     1201 catgaccttg agggcggtct ggccgacgaa ccccgagacc agcccggagc ggtcggtctc
     1261 cacgagctgg ccccgctcca ccacgccgag cgagcggtag atcgccgcca ggaggcgggc
     1321 gacggtggtc ttgccggtgc cggggttccc ggtgaagacc aggtggcggg actgtggcgt
     1381 cgtgggcagc ttccgctcgg tgcggagctg ctggacgcgc aggaggttgg tgacgaggcg
     1441 cacctcggtc ttgacgctgt cgagtccgac caggtcgtcg agctcggcga gcagctcctc
     1501 gatcggccgg gcgggaggga gcggttcggc ggccggcgct gccgtgccgc ccgccgcgat
     1561 ggcggcctgg tcggcgccgg cggcggcggg ggtgccggtc gcgggcggtc cgctgcgagg
     1621 cggcaggggc gacgacggca ggccggacat ggcctggagc atctggttgc ggaactcgtc
     1681 gatcgcctgc agctcggcgc gtgacggcgc cgcctccagg gcggccacgg tgaacgcgag
     1741 gttcagcgac cggtcgtagt aggtccggct gtggctggtg ccgaagcgcc gatcggccgt
     1801 gaccaggatc tcgaacatgg gcgacacctg gtccaaccag cccgctcggc ccacgaggag
     1861 gtcgctgcgc cgcaggtcgt cgggcttggc tcgggcgagc tgcgccggca gccagcggcc
     1921 gaaggcgaag atcaggcccc acagctcgtc gtcggtgtgg ctgcgatcga cgtcgatgag
     1981 cgccgtggtg aggtggaacg cctcggtggc gacgtcgcgg tccatctcct tgctgtcgag
     2041 atccggcagg cgagcgacga cgtcgcgcat cgcgggcgcg atctcggcca cgaacgcctc
     2101 gaccgcccgc tgcatctgct tcggctcgct cagcacggcc ccgtcatccc cgcgggtccc
     2161 gccacatcag ccagatcggc gggccaccgc cgggagcgat ctcctcgcgc acctcgaacc
     2221 cgtgccgggc gtagaagggg acgttctgct ccttcgacga ttccaggtac gcccccaggc
     2281 cctgctcgtc gcagcggtcg gtgacggctc ggatgagggc gctcccgacg cccgacccct
     2341 ggtgctcggg atcggtgccg aggatcgcca ggtaccagtg cggcgggtcc ttcgggtgct
     2401 tgcgctccat ggccgccagg tcggcgatgg cccggcggag ccgtcctcgg aacaggcgca
     2461 ccgaggggat ggcgagcgcc agcccctccg acacggtgcc cctccagtgc cccggcgggg
     2521 cccagatggc cgcgccccgg acctggtggt cgacgaggac ctcgccgtgg ccgcggtgct
     2581 gcgaccgcgc ctcggcctcg aaccacgccg cggcccgcgt ggaccagtcg gccttgggcg
     2641 aggccaggtg gctccagacc gggtcgccgt cgaacgccgc ggcgagcgcg cggccgaccg
     2701 caggcaggtc agcagcggtg gcggggcgga tgacaggcac ggtcgcca
//
LOCUS       DATMFS010000288         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_241589, whole genome shotgun sequence.
ACCESSION   DATMFS010000288 DATMFS010000000
VERSION     DATMFS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241589"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..538
                     /locus_tag="VNQ33_05295"
     CDS             107..538
                     /locus_tag="VNQ33_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWJ97553.1"
                     /translation="MASSTPPASHAHLLEQPLIAHLGTVRPDGSPQVSEMWFDWDGQV
                     ARFTHTTKRQKFKNFEHEPRVAFSIADPDDPYTFLEVRGAVESIEPDPNADFYVSLQR
                     AYGLQIGRGDADVRVVITIRPTGFVGVTGGQTEAEQAASKH"
     gene            complement(740..>3639)
                     /locus_tag="VNQ33_05300"
     CDS             complement(740..>3639)
                     /locus_tag="VNQ33_05300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWJ97554.1"
                     /translation="SVYRAFDDAFDRFVAVKVIDRDVPFELSSEARTLGRLGEHPHIA
                     SVFSIGRLADGRAYLTMPFYPRGDLGGLIQREGPLREGSLLDIGIKLSGALETAHQHG
                     VIHGDVKPANVFLTRYDDVVLADFGISHGLQETTLGYTPAFAAPEILGGSQSSVRSDV
                     YALCVTLYLAATGRHPFPVDASGRRLGPDPGGSDQLVDVDSLTSEAAEAIAAGLAEDP
                     AERLPSALDIGTALQAAQQRLHIPPTALLVGDDVHDVTGYWSTGRDASVIAPGTLWRG
                     GSSTAGPVSHPLPGWLTDTGFPEGDQPRRHAEAASPVASEPIPPPPMDHGTVVAPGER
                     TYLPPKGETGSPFAENTAGSTASRPPAVGAVGAPPPPPPPPPPPPPGGPGFGGLAHEP
                     APSPAGRGCLAWPVAVITALLGAIGSWFGGWRTESSNGDSVPRDDRSGSLYAFSPDRP
                     TEVDRLGHEPLVDGIVRVLNDPGTGFPLTVAIAGRWGAGKSSTMLQLRRGLAAGDGGQ
                     RWLPVWFDAWRHQGRDALWVGLARAMYRAGQADSGGFLRRAWFRVRLERRRVGVAKLV
                     CGGCVVLVGLAYLCRAVAEGLRGSSDWWLLSGSGAAAAAVAVSAAYLGVITAPFERAV
                     ARASGTRRYDDALGITDAAERDVECLIDTLTGRGKRTVVVFLDDLDRCQPGVIVEALA
                     TVTEIFSRREDQSIVFVLGVDMDVVVGAIESSLEEVRRSIDQFDPRRAANIGRDFLER
                     IFQLTVVLDGVGSPPVGRLLVGESQASVDVAVVDAMVRRLKGIDLANPGELTEVRRSA
                     GLNTLDVAGNVRELRIAVRERRADLLSINSEQVRNAELQIVSVLRLSPRALKRFDNTY
                     RLQLQVSNGTPGCQLHYGPDELLALAKWVALRLFFGRVSSILDREFGLLPDLERLARR
                     TDGGAEFGALLTRVAGELSPSEVTELLALLSTGGPSTSVSQLPFDSFSTTV"
BASE COUNT          628 a         1278 c         1220 g          513 t
ORIGIN      
        1 ggcagagcat cggactttta atccgctggt tccgggttcg atccccgggc ggcccaccag
       61 accccaccac gtcggaatcg aggtggacca tccataccgt gggctcatgg cctcttccac
      121 tcctcccgct tctcacgccc acctgctcga gcagccgctg atcgcccacc tcgggacggt
      181 tcgcccggac gggtcgccgc aggtgagcga gatgtggttc gactgggacg gtcaggtcgc
      241 ccggttcacg cacacgacga agcggcagaa gttcaagaac ttcgagcacg agccgcgggt
      301 ggcgttctcc atcgccgatc ctgacgaccc gtacacgttc ctcgaagtgc ggggcgccgt
      361 cgagtcgatc gagcctgatc cgaacgccga cttctacgtg agcctccaga gggcctacgg
      421 cctgcagatc ggccggggcg atgccgatgt ccgcgtcgtg atcacgatcc gcccgaccgg
      481 cttcgtcggc gtcaccggcg ggcagaccga agccgagcag gccgcctcca agcactgatc
      541 cgtcctggcg ccggtacgcc ggttgtcgct ccgaagcgaa ccagcggact gccacgagct
      601 cccggtgccg tcacctcgtg gcgacgcggt tgaccggttc ggccgacggc ttcgatgtgc
      661 tgttcggtct ctgcaacgcg acccgcggcg tccgcccagc gccgaggtgc tcgagcacga
      721 ggcctcggcg agcgcctgct cacaccgtgg tcgaaaacga gtcgaacgga agctgcgaga
      781 ccgaagtcga tggtccaccc gtgctgagga gggcgagcag ctccgtgacc tcgcttggcg
      841 acagctcacc cgcgacccgc gtgaggagcg ccccgaactc ggcgccaccg tcggtgcggc
      901 gggcgaggcg ttcaagatcc ggcagcagcc cgaactcgcg atcgaggatc gacgaaacgc
      961 gaccgaagaa cagccgcagc gcgacccact tggccagtgc gagcagctcg tctgggccgt
     1021 agtggagctg gcaccccggc gttccgttcg aaacctgcag ctgcaagcgg taggtgttgt
     1081 cgaagcgctt cagcgcgcgc gggctgagtc ggagcaccga cacgatctgc agctcggcgt
     1141 tccgtacttg ctcggagttg atcgagagca ggtcggctcg ccgttcgcga acggcgattc
     1201 gcagctctct cacgttgccg gcgacgtcca aggtgttcag ccctgccgat cgtcgcacct
     1261 cggtcaattc gccaggattg gcgaggtcga tgcccttcag gcgccggacc atcgcatcga
     1321 ccaccgcaac gtcgacgctg gcctgggact cgccgacgag cagccggccg accgggggtg
     1381 agcccactcc atcgaggaca accgtgagct ggaagatgcg ctcgaggaaa tcgcgaccga
     1441 tgttcgcggc ccgcctggga tcgaactggt cgatcgagcg ccgcacctcc tcgagcgacg
     1501 actcgatcgc cccgacgacg acatccatgt ccacgccgag gacgaacacg atcgactggt
     1561 cctcacgtcg gctgaagatc tccgtgaccg tggccagcgc ctcgacgatg acacccggtt
     1621 ggcaccggtc caggtcgtcc aagaacacca cgacggtgcg cttcccgcgg ccggtgagcg
     1681 tgtcgatcaa gcactcaacg tctcgttccg cggcgtcggt gatgccgagc gcgtcgtcgt
     1741 agcgcctcgt gccagacgcc cgcgcgaccg cacgctcgaa cggcgccgtg atcacaccga
     1801 ggtacgccgc agagaccgca accgctgcgg ctgccgcacc ggatccggag agcagccacc
     1861 agtcggagct gccccgcaaa ccctcagcaa ccgcccggca cagataggcc agtcccacga
     1921 ggaccacgca cccaccgcac acgagcttcg ccacaccgac acggcgccgc tcgagccgca
     1981 cacgaaacca agcacgccgg aggaagcccc cgctgtcggc ctgtccagcg cggtacatgg
     2041 cccgagccag gccgacccac agcgcatcgc gcccctggtg cctccatgcg tcgaaccaga
     2101 cgggaagcca gcgctgtccc ccatcgccag cagcgagccc gcgtcgcagc tgcagcatgg
     2161 tcgacgactt tccagctccc caccggccgg cgatggcgac cgtcaggggg aatcccgttc
     2221 ccgggtcgtt caagacacgg acgatcccgt cgacgagcgg ttcgtggccg agccgatcca
     2281 cctccgtggg ccgatcgggg ctgaaggcgt acagcgaacc agaacggtcg tcccgaggca
     2341 cgctgtcgcc gttcgagctc tcggtgcgcc agccgccgaa ccaagacccg atcgcaccga
     2401 ggagggcggt gatcacggcc acgggccacg cgaggcatcc tcgtccggcg ggactcgggg
     2461 caggctcgtg tgccagaccg ccgaatcctg ggcctcctgg cggtggaggc ggtggaggcg
     2521 gtggaggcgg tggaggcgcc ccaaccgctc caaccgccgg cggtcgagag gcggtggacc
     2581 ccgccgtgtt ctcggcgaac ggagatccgg tctccccctt cggggggaga tacgtacgtt
     2641 cgccgggggc gaccacggtc ccgtggtcca tcggcggcgg cgggatcggc tccgatgcca
     2701 ccggcgatgc ggcctccgca tgccttctcg gctggtcacc ctctggaaac ccggtgtcgg
     2761 tcagccaccc cggcagcggg tgcgacactg ggcccgccgt actcgatccg ccacgccaga
     2821 gggtgcccgg tgcgatgacg ctggcgtcgc ggccggtcga ccagtacccg gtcacatcgt
     2881 ggacgtcgtc gcccacaagg agggcggtcg gcgggatgtg caagcgctgc tgtgcggcct
     2941 ggagcgccgt cccgatgtcg agagcgctcg ggagacgttc ggcaggatcc tccgcaagac
     3001 ccgccgcgat cgcctctgcg gcctccgacg tgagggagtc cacgtcgacc agctggtccg
     3061 aacctcccgg atcgggaccg aggcgccggc cagaagcgtc cacggggaag ggatggcgac
     3121 cagtggcagc gaggtagagg gtgacgcaca acgcgtagac atcggaacgt acagacgact
     3181 gcgatccgcc gaggatctcc ggcgcagcga acgccggcgt gtagccgagc gtcgtctcct
     3241 gcaggccgtg tgagatcccg aagtccgcga gcacgacatc gtcgtaccgg gtcaagaaca
     3301 cgttcgccgg cttcacatcg ccgtggatga ccccgtgctg gtgggcggtc tccagggcgc
     3361 cgctcagctt gatgccgatg tccagcaggg acccctcccg aagaggaccc tcccgctgga
     3421 tcaggccacc cagatcacca cgggggtaga agggcatggt caagtaggcg cggccatcgg
     3481 caagccggcc gatcgagaac accgacgcga tgtgcgggtg ctctccaagg cggccgaggg
     3541 tgcgagcctc ggagctcagc tcgaacggaa cgtcgcgatc gatgaccttc acggcgacga
     3601 agcggtcgaa cgcgtcgtcg aaggcgcggt acaccgacg
//
LOCUS       DATMFS010000289         2816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80143, whole genome shotgun sequence.
ACCESSION   DATMFS010000289 DATMFS010000000
VERSION     DATMFS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80143"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1930
                     /locus_tag="VNQ33_05305"
     CDS             35..1930
                     /locus_tag="VNQ33_05305"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97555.1"
                     /translation="MAHFQDRIDRALVRGQINVVFWLGGAALLLAATAALIVTVLHLA
                     VGGHDPSFEEAFWQSLVRTIDPGQITEDHAGFAVVGLTITIGGLLLISTLISLVNNRI
                     ERRVEGIRRGRDPIPPRADHVVVLGWSEIGPKVVEELADSGDGEKLIDLVVLADRSVT
                     DVRHELVDHEDLADRAVHWPVLRSGATWDTRDLRRLASVATARAVVILDDGTEDGLAN
                     TVKSVMAVVAACDGPDHGREPHEHPTIVIEVRDPHNELVEPLRGRLARFGFRVVAVDS
                     LALRTELAAQVSRRAGLSQVFHEILNFRGEELYIAAAPDVVTTFGQALTALQESVPIG
                     LISGHDGAVDLWPDWSTPLAGHQLVVIATDETVAAADGPAALDAEAVSMAGLRPSCCE
                     RPLERQQLLVVGWNDGAVHLLEVLDQYAGDGSAVTVVTDEPVGTDRLKLESFTDQRFL
                     CPDIGVQAWLDLQRDQFDHAIVLSDDRLSAAGSDAAAFVTLLSLRPAGDSHGNPTTVV
                     AQLRQRENKHLARRSLADDLVVGDAVTATTLAQLAMNPDLDAVLDQILGDTPFTVELV
                     ANPYLGRHRDVTFAEITTAVAEHGEIAIGWIRVDGTMKLAPPKGERISHEDLQQIVVF
                     TRLRLGG"
     gene            complement(1949..>2816)
                     /locus_tag="VNQ33_05310"
     CDS             complement(1949..>2816)
                     /locus_tag="VNQ33_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ97556.1"
                     /translation="DEHEIPHVHDSYAALLADPEIDAVYNPLPNGLHGEWTIAALEAG
                     KHVLCEKPFTSNAAEAREVKAVADAHPDLVVMEAFHYRYHPFAERLRAIAASGELGTI
                     ERVETAMCFPLLKRDDIRWDDGLAGGATMDVGCYTIHCLRLLAGEEPEVVSAEAKLRG
                     PNVDRAMVAELRFPSGATGRIHCSMWSSSILKLGARVTGSEGEMKVRNFTMPHLLGKV
                     TVSTKAGTRSEAGSKDRTYARQLDAFVRTVGGDASANLTPPSDSVTTMEIIDAVYEAA
                     GLPVRGTAPSTA"
BASE COUNT          390 a         1029 c          981 g          416 t
ORIGIN      
        1 atgagcagtg gtacgagccc ggcggcagaa gcggatggcg cacttccagg accggatcga
       61 ccgggccctg gtgcgcggcc agatcaacgt cgtcttctgg ctcggcgggg ccgcgctcct
      121 cctcgctgcg accgcggcgc tcatcgtcac cgtcttgcac ctcgccgtcg gcggtcacga
      181 cccgagcttc gaggaggcct tctggcagtc gctggtccgc acgatcgacc cgggccagat
      241 caccgaggac cacgccggct tcgccgtggt cgggctgacc atcaccatcg gcggcctcct
      301 gctcatctcg acgctgatct cgctggtcaa caaccgcatc gaacgacggg tcgagggcat
      361 ccgccgcggc cgggacccga tcccgccccg ggccgaccac gtggtcgtgc tcggctggtc
      421 ggagatcggg ccgaaggtgg tggaggagct cgccgacagc ggtgacgggg agaagctgat
      481 cgacctggtg gtgctggccg atcggagcgt caccgacgtg cgccacgagc tggtcgacca
      541 cgaggacctc gccgaccggg cggtgcactg gccggtgctc cggtccggcg ccacgtggga
      601 cacgcgcgac ctgcggcgcc tcgcgtcggt ggccacggcc cgagcggtcg tcatcctcga
      661 cgacggcacc gaggacgggc tggcgaacac cgtgaagtcg gtgatggcgg tcgtggccgc
      721 gtgcgacgga ccggaccacg gccgcgagcc gcacgagcac cccacgatcg tgatcgaggt
      781 gcgcgacccg cacaacgagc tggtggagcc gctgcgaggc cgtctggcgc ggttcgggtt
      841 ccgggtcgtg gcggtcgact cgctggcgct gcgcacggag ctggccgccc aggtctcccg
      901 gcgggccggc ctctcgcagg tcttccacga gatcttgaac ttccgcggcg aggagctcta
      961 catcgccgct gcgcccgacg tcgtcaccac cttcggccaa gcgctcacgg ccctgcagga
     1021 gtcggtgccg atcgggctca tcagcggcca cgacggcgcc gtcgacctct ggccggactg
     1081 gtccacgccg ctcgccggcc accagctcgt cgtgatcgcc accgacgaga ccgtcgcggc
     1141 cgccgacggc cccgccgcgc tggatgccga ggccgtctcg atggcgggtc tccgcccgtc
     1201 gtgctgcgag cgcccgctcg agcgccagca gctgctcgtc gtcggctgga acgacggggc
     1261 ggtccacctg ctcgaagtgc tcgaccagta cgccggcgac ggctcggccg tcacggtcgt
     1321 gaccgacgag cccgtcggga ccgaccggct caagctcgag agcttcaccg accagcgctt
     1381 cctctgtccc gacatcgggg tgcaggcgtg gctggacctc cagcgcgacc agttcgacca
     1441 cgccatcgtc ttgtccgacg accgcttgtc tgccgccggc tcggatgcgg cggccttcgt
     1501 caccctgctc tcgctgcgtc ccgccggcga cagccacggg aacccgacga cggtcgtcgc
     1561 ccagctgcgc cagcgcgaga acaagcacct cgcccgcagg agcctggccg acgacctggt
     1621 ggtgggcgat gcggtcaccg ccaccaccct cgcccagctc gccatgaacc ccgatctcga
     1681 cgcggtgctg gaccagatcc tgggcgacac gccgttcacg gtcgagctcg tcgccaaccc
     1741 ctacctgggc cggcaccgag acgtgacctt cgccgagatc accaccgcgg tggccgagca
     1801 cggcgagatc gccatcggct ggatccgcgt ggacggcacg atgaagctgg caccgccgaa
     1861 gggcgagcgc atctcgcacg aggacctgca gcagatcgtg gtcttcaccc ggctccgcct
     1921 cggcggctga ccggttcggt cccgcccgtc aggcggtgga cggcgcggtc ccccgcaccg
     1981 ggagcccggc cgcctcgtag accgcgtcga tgatctccat cgtggtgacc gagtcgctcg
     2041 gcggcgtgag gttggcgctg gcgtcgcccc cgaccgtgcg gacgaaggca tcgagctggc
     2101 gggcgtacgt gcggtccttg gacccggcct cgctgcgggt ccccgccttg gtggagacgg
     2161 tcacctttcc gaggaggtgg ggcatcgtga agttccggac cttcatctcg ccttcggagc
     2221 cggtcacccg ggcaccgagc ttgaggatcg acgaggacca catcgagcag tggatgcgcc
     2281 cggtcgcccc gctggggaac cgcagctcgg cgaccatcgc ccggtcgacg ttcgggcccc
     2341 tcaacttggc ctcggccgac acgacctccg gctcctcgcc agccagcagc cgcaggcagt
     2401 ggatcgtgta gcagccgaca tccatggtgg ccccgccggc cagaccgtcg tcccagcgga
     2461 tgtcgtcgcg cttcaggagc gggaagcaca tcgccgtctc gacccgctcg atcgtgccga
     2521 gctcgccgct ggcggcgatg gcccggaggc gctcggcgaa cgggtggtag cggtagtgga
     2581 acgcctccat caccacgagg tcggggtggg cgtcggccac cgccttcacc tcccgagcct
     2641 cggcggcgtt cgacgtgaac ggcttctcgc acaagacgtg cttgcccgcc tccagcgcgg
     2701 cgatggtcca ctccccgtgc agaccgttgg gcagcgggtt gtagaccgcg tcgatctcgg
     2761 gatccgccag gagggcggcg tagctgtcgt gcacgtgcgg gatctcgtgc tcgtcg
//
LOCUS       DATMFS010000290         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_150488, whole genome shotgun sequence.
ACCESSION   DATMFS010000290 DATMFS010000000
VERSION     DATMFS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150488"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VNQ33_05315"
     CDS             complement(<1..298)
                     /locus_tag="VNQ33_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWJ97557.1"
                     /translation="MPDAGTDVRATHVVLVGLMGSGKTTVGKKVAKLTGHRFVDADVE
                     IEARTGRSVASWFEDGEAAFRGAEADLLEVLLASPEALVVGAGGGVVVTESNRRR"
     gene            complement(291..1478)
                     /gene="aroC"
                     /locus_tag="VNQ33_05320"
     CDS             complement(291..1478)
                     /gene="aroC"
                     /locus_tag="VNQ33_05320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWJ97558.1"
                     /translation="MIRYLTAGESHGKALIVVIEGVPAGLHLAVADIASELSRRRLGY
                     GRGPRMRFEQDQVTLVGGVRHGRTLGSPIAIEIANTEWERNPEKWGTEMSPRAEDGPT
                     QAPLTQIRPGHADLAGMQKYGFTDARDVLERASARETAARVAAGTVAKALLAQLGVQI
                     VSHVIQMGPARSASGERPGPADLPRVDENEVRCLDLDAAAAMVAEIKEAAKEGDSLGG
                     VVEAIAYGVPIGLGSHVHWDRKLDSALAQAVMSIQAVKGVEVGDGFDVAGRRGSDAHD
                     PVTWTDAEGYERSSNFSGGVEGGISTGEPVVVRAAMKPLATLNRPTLATVDTATKEAG
                     VSFKERTDVTAVPALGVVVEHMVALVLANEAVRKFGGDSVDELVRNHRAFVDALEQGP
                     VGA"
     gene            complement(1489..2343)
                     /locus_tag="VNQ33_05325"
     CDS             complement(1489..2343)
                     /locus_tag="VNQ33_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HWJ97559.1"
                     /translation="MPTGLLLVWLALFGLVIGSFLNVVIVRVPSGESVMSPPSKCPLC
                     EQPIRPRDNIPVVSWLLLKGRCRNCGEPIPAGYPLVEAGNAVLWVLAGVAFGTSWWVI
                     PYALLFSVLLALSVIDLELYILPNRITYPTILASLVAIPALAFVLSDDPRSVILGALV
                     GGIGFAGFLLVMLLGWELILRKEGMGMGDVKLAVILGMWLGLINPLLCLFALIASSLI
                     GLVVGIGILIVRKESRPFPFGPWLAIGTILVILFSGPILRAYDIDADETAAPLPPASA
                     SLPVVIGQ"
BASE COUNT          374 a          856 c          851 g          306 t
ORIGIN      
        1 ggcggcgtcg gttcgactcg gtgacgacga ccccgccgcc cgcgcccacc accagggcct
       61 cggggctggc cagcagcacc tcgagcaggt cggcctcagc gccgcggaac gccgcctcgc
      121 cgtcttcgaa ccacgaggcg accgagcgcc cggtgcgggc ctcgatctcc acgtcggcgt
      181 cgacgaaccg gtggccggtc agcttggcga ccttcttgcc gaccgtggtc ttgcccgaac
      241 ccatcaggcc gacgagcacg acgtgggtgg cccggacgtc ggtgccggcg tcaggcaccg
      301 accgggccct gttccagcgc gtcgacgaag gcgcggtggt tgcggaccag ctcgtcgacc
      361 gagtccccac cgaacttgcg gacggcctcg ttggccaaga cgagagcgac catgtgctcg
      421 accaccacac cgagggccgg gacggcggtg acgtcggtgc gctccttgaa cgacacgccc
      481 gcctccttgg tggcggtgtc gaccgtggcg agggtgggcc ggttcaacgt ggccagcggc
      541 ttcatggccg cccgcaccac gacgggctcg ccggtggaga tgccgccctc gacgccgccc
      601 gagaagttcg agctgcgctc gtagccctcg gcatcggtcc aggtgaccgg gtcgtgggcg
      661 tcgctccccc gccggccggc gacgtcgaag ccgtcgccga cctcgacgcc cttcacggcc
      721 tggatgctca tcaccgcctg ggccagcgcg ctgtcgagct tgcggtccca gtggacgtgg
      781 ctgccgaggc cgatcggcac gccgtaggcg atggcctcga ccacgccgcc gagcgaatcg
      841 ccctccttcg ccgcctcctt gatctcggcc accatcgccg ccgccgcgtc gagatcgagg
      901 cagcggacct cgttctcgtc gacgcgcggc aggtcggcgg ggcccgggcg ctcgcccgag
      961 gcggaacggg ccggacccat ctggatcacg tgcgagacga tctggacgcc gagctgagcc
     1021 agcagcgcct tggcgacggt gcccgccgcc acccgcgccg cggtctctcg cgccgaggcc
     1081 cgctccagga cgtcgcgcgc atcggtgaag ccgtacttct gcatgccggc gaggtcggcg
     1141 tggccggggc ggatctgggt gagcggggcc tgggtggggc cgtcctcggc ccggggcgac
     1201 atctcggtcc cccacttctc cgggttgcgc tcccactcgg tgttggcgat ctcgatggcg
     1261 atcggcgacc ccagcgtgcg gccgtggcgg acgccgccca ccagggtgac ctggtcctgc
     1321 tcgaagcgca tgcgggggcc gcggccgtag ccgaggcggc ggcggctcag ctccgatgcg
     1381 atgtcggcga ccgccaggtg cagccccgcc gggacgccct cgatgacgac gatgagggcc
     1441 ttgccgtggg attccccggc ggtgaggtag cggatcacct agccaaacct actggccgat
     1501 caccaccggc aacgaagcgc tcgcgggcgg cagcggcgcg gccgtctcgt ccgcgtcgat
     1561 gtcgtacgca cgcaggatcg ggcccgagaa gaggatcacc aagatggtcc cgatggccag
     1621 ccacgggccg aacgggaacg gccggctctc cttgcggacg atcaagatcc cgatgccgac
     1681 gaccagcccg atcaggctcg aggcgatgag ggcgaacagg cacaggagcg ggttgatgag
     1741 gcccagccac atgcccaaga tcacggccag cttcacgtcg cccatgccca tcccctcctt
     1801 gcggaggatg agctcccagc ccagcagcat caccaggagg aagccggcga acccgatgcc
     1861 gccgacgagc gcacccagga tcacgcttct cggatcgtcg gacagcacga aggcgagcgc
     1921 ggggatggcg acgagcgagg cgaggatcgt cgggtaggtg atccggttgg ggaggatgta
     1981 cagctcgagg tcgatgaccg acagggccag cagcaccgag aacagcaggg cgtaggggat
     2041 cacccaccac gacgtgccga aggcgacgcc ggccagcacc cacagcacgg cgttgccggc
     2101 ctcgaccaac gggtagcccg ccgggatcgg ctcgccgcag ttgcggcacc ggcccttgag
     2161 cagcagccag gagacgaccg ggatgttgtc ccgcggccgg atgggctgct cgcacagcgg
     2221 gcacttcgac ggcggcgaca tgaccgactc acccgacggc acccggacga tcacgacgtt
     2281 gaggaacgag ccgatgacca gcccgaacaa ggcgagccac acgagcagga gtccggtcgg
     2341 catggtgcgg acgctaccgg tcggcccgac ccgcgccggg catggcc
//
LOCUS       DATMFS010000291         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_34172, whole genome shotgun sequence.
ACCESSION   DATMFS010000291 DATMFS010000000
VERSION     DATMFS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34172"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VNQ33_05330"
     CDS             <1..334
                     /locus_tag="VNQ33_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWJ97560.1"
                     /translation="GAVGRDGEALVLDMGEPVKIDDVAKRLISDTGRPIDVVYTGLRP
                     GEKLHEVLFGADEQGSSPIHPRIAHVAVPPLSARQLPVPQPDATDAQTIAVLREAVTR
                     QDIRSTAI"
     gene            complement(344..1699)
                     /locus_tag="VNQ33_05335"
     CDS             complement(344..1699)
                     /locus_tag="VNQ33_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ97561.1"
                     /translation="MSRTRLTGWANTAPTASDVVRPHDADELAAALDLDAPRGVIARG
                     LGRSYGDPAQNAGGLVVDATQVTGISDLDIQRGIVTAEAGTSLDALMRWLVPLGWFVP
                     VTPGTRQVTVGGAIASDIHGKNHHKAGSWCDAVEAITLVTPAGGRRTITPKSDPDLFW
                     ATAGGMGLTGVIVDATFRLNRIETSRLSVDTDRTPDLDTTLDLMESGDHGYDYSVAWI
                     DLMAKGASMGRSILGRGRFATIDQLGARQRRDPLHFTSGTIATAPSLVPPKLINPLTV
                     KAFNEAWYRKAPRRKRDQVMGITEFFHPLDLVDRWNRLYGREGLLQWQFVLPLTATEE
                     LRTIIGELSRSGCPSFLAVLKRMGPGNAGPLSFPIEGLTLAFDVPADAGSGLGPLLDR
                     LDERVAAAGGRLYLAKDSRMRPELLPVMYPRLDEWLAVRHEVDPDQRLVSDLARRLRL
                     V"
     gene            complement(1696..2577)
                     /locus_tag="VNQ33_05340"
     CDS             complement(1696..2577)
                     /locus_tag="VNQ33_05340"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HWJ97562.1"
                     /translation="MPRSLPRALLAEARPKQWVKNLLVFAAPAAAGVIDEPKALGQTV
                     AAFACFCLAASGTYFLNDAADVEADRLHPKKRFRPIAAGEIPLPMARVVGVAAIVGSV
                     LAALLVNWHLAATVGAYVALTTTYSLWMKHVAVLDVVGVAAGFVLRAIGGATAVDVPI
                     SDWFFIVASFGSLLMVVGKRRAETDEMGEAAAEFRPTLGMYPAGYLAQLQTVATAVVL
                     VGYCLWAFEKAALSGSSVPWFELSIVPFAVAILRYVLLLDSGQGGAPEDVILGDRALQ
                     IMGGIWLVVFAIGVYAT"
     gene            complement(2628..>3174)
                     /locus_tag="VNQ33_05345"
     CDS             complement(2628..>3174)
                     /locus_tag="VNQ33_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWJ97563.1"
                     /translation="AESKPFGFMPFRPGPGVGGHCLPIDPSYLSWRVKQSLGQTFRFV
                     ELANDVNEHMPDYVIRRLVAALNDRELSVKGRRVLVLGLAYKKNTGDDRESPSTRVIE
                     LLSELQADLAVCDPHVDPARSHAIGTAEHVELTEAEVAKADAIVVLVDHDAFDLDMVA
                     RSGTYILDTRRCIEGPAVEHL"
BASE COUNT          465 a         1080 c         1206 g          423 t
ORIGIN      
        1 gggcgcggtc ggccgcgacg gcgaagccct cgtcctcgac atgggcgagc ccgtgaagat
       61 cgacgacgtc gccaagcggc tcatcagcga caccggccgc ccgatcgacg tcgtctacac
      121 cggccttcgc ccgggcgaga agctgcacga ggtgctcttc ggcgccgacg agcagggctc
      181 gtcgccgatc cacccccgca tcgcccacgt ggccgtgccg ccgctgtcgg ctcgccagct
      241 cccggtcccg cagcccgatg ccaccgacgc ccagaccatc gccgtcctgc gcgaggccgt
      301 cacccgccag gacatccgca gcaccgccat ctgacggcgg ggctcagacc aggcggagcc
      361 ggcgggcgag gtcgctgacc aggcgctggt cggggtcgac ctcgtggcgc acggcgagcc
      421 actcgtcgag ccgggggtac atcaccggga gcagctcggg acgcatccgg ctgtccttgg
      481 ccaggtagag gcggccgccg gcggccgcca cccgctcgtc gaggcggtcg agcagcggcc
      541 cgagcccgct gccagcgtcg gcgggcacgt cgaacgcgag ggtcagcccc tcgatcggga
      601 acgacagggg accggcgttg ccggggccca tgcgcttgag cacggcgagg aacgacgggc
      661 agcccgaccg gctgagctcg ccgatgatcg tccgcagctc ctcggtggcg gtgaggggca
      721 ggacgaactg ccactgcagc aggccctccc gcccgtagag gcggttccag cggtcgacga
      781 ggtcgagcgg gtggaagaac tcggtgatgc ccatcacctg gtcgcgcttg cgacgcggcg
      841 ccttgcggta ccaggcctcg ttgaaggcct tgacggtcag ggggttgatg agcttcggcg
      901 gcaccagcga cggggcggtg gcgatggtgc ccgaggtgaa gtgcagcggg tcgcgccgct
      961 ggcgggcgcc cagctggtcg atcgtcgcga agcggccgcg gccgaggatc gagcgcccca
     1021 tcgacgcgcc cttggccatc aagtcgatcc aggcgaccga gtagtcgtag ccgtggtcgc
     1081 ccgactccat caggtcgagg gtcgtgtcca ggtccggggt gcggtcggtg tcgacgctga
     1141 gccggctggt ctcgatccgg ttcaggcgga acgtggcgtc gacgatcacc ccggtgaggc
     1201 ccatgccgcc ggccgtggcc cagaacaggt cgggatcgga cttgggggtg atggtccgcc
     1261 gaccgccggc cggggtgacg agcgtgatgg cctcgacggc gtcgcaccac gacccggcct
     1321 tgtggtggtt cttgccgtgg atgtccgacg cgatcgctcc gccgacggtg acctggcggg
     1381 tcccgggcgt gacgggcacg aaccagccga gcgggacgag ccaccgcatc agggcatcga
     1441 ggctggtgcc ggcctcggcc gtgacgatcc cgcgctggat gtcgagatcg ctgatcccgg
     1501 tcacctgggt cgcgtcgacc accaggcctc cggcgttctg cgccgggtcg ccgtaggacc
     1561 ggcccaggcc gcgggcgatg acgccccgcg gcgcgtcgag gtcgagggcc gccgccagct
     1621 cgtcggcatc gtggggccgg accacgtcgg aggccgtggg cgcggtgttg gcccagccgg
     1681 tgaggcgagt gcggctcatg tcgcgtacac gccgatcgcg aacacgacga gccagatccc
     1741 gcccatgatc tggagcgcgc ggtcgccgag gatgacgtct tcgggcgcgc cgccctgccc
     1801 gctgtcgagg agcaggacgt agcggaggat cgccaccgcg aacggcacga tcgacagctc
     1861 gaaccacggg acgctcgatc cggagagcgc cgccttctcg aacgcccaca ggcagtagcc
     1921 cacgagcacc acagcggtgg cgaccgtctg cagctgggcg aggtagccgg ccgggtacat
     1981 ccccagcgtg ggccggaact cggcggcggc ctcgcccatc tcgtcggtct cggcccggcg
     2041 cttgcccacc accatcagca gcgagccgaa cgacgccacg atgaagaacc agtcggagat
     2101 cgggacgtcg acggcggtgg caccgccgat ggcgcgcagc acgaacccgg cggccacgcc
     2161 gacgacgtcg agcacggcga cgtgcttcat ccagaggctg taggtggtgg tgagcgccac
     2221 gtacgcgccc acggtggccg ccaggtgcca gttcaccagg agggcggcga ggaccgagcc
     2281 gacgatcgcg gccaccccga cgacgcgggc catcggcagc gggatctcgc cggcggcgat
     2341 ggggcggaac cgcttcttcg ggtggagccg gtccgcttcg acgtcggccg catcgttcag
     2401 gaagtaggtg ccggacgcgg ccaggcagaa gcaggcgaac gcggcgacgg tctggcccag
     2461 cgccttgggt tcgtcgatga cgccggcggc ggccggggcc gcgaacacga ggaggttctt
     2521 gacccactgc ttggggcgtg cctcggcgag cagggcacgt ggcagggatc ggggcatcga
     2581 cgcagcctag agggggtggt gcgtgccgct tcggacgggc gcccgtgcta caggtgctcg
     2641 acggccggac cctcgatgca ccggcgggtg tcgaggatgt acgtgccgga tcgggcgacc
     2701 atgtccaggt cgaacgcgtc gtggtccacc aagacgacga tggcgtcggc cttcgccacc
     2761 tcggcctcgg tgagctcgac gtgctcggcc gtcccgatgg cgtggctgcg ggccgggtcc
     2821 acgtgcgggt cgcagacggc gaggtcggcc tgcagctccg agagcagttc gatgacccgg
     2881 gtgctgggcg actcgcggtc gtcgccggtg ttcttcttgt aggccaggcc gagcacgagc
     2941 acccgccggc ccttgaccga cagctcgcgg tcgttgaggg cggcgacgag gcggcggatg
     3001 acgtagtcgg gcatgtgctc gttgacgtcg ttggccagct cgacgaaccg gaaggtctgc
     3061 ccgagcgact gcttgacgcg ccaggacagg taggacgggt cgatgggcag gcagtgcccg
     3121 cccacgcccg ggcccgggcg gaacggcatg aagccgaagg gcttgctctc ggcg
//
LOCUS       DATMFS010000292         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_11204, whole genome shotgun sequence.
ACCESSION   DATMFS010000292 DATMFS010000000
VERSION     DATMFS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11204"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VNQ33_05350"
     CDS             <1..319
                     /locus_tag="VNQ33_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWJ97564.1"
                     /translation="NGFTFSITLGVMSGTTWIPVSAERRKGAGISAGETVEVAMVVDD
                     APKVIEVPGDLAAALEHAGARAAFDALAPSHRKEHVRAVTEAKKPETRERRIAKCVEM
                     VRP"
     gene            complement(394..1962)
                     /gene="serA"
                     /locus_tag="VNQ33_05355"
     CDS             complement(394..1962)
                     /gene="serA"
                     /locus_tag="VNQ33_05355"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWJ97565.1"
                     /translation="MARVLVSEKIADGGLDALRAAGHDVDVQLGLSPEELVDAIKGAH
                     ALIIRSATTVTKEVLDAGTDLVVVGRAGIGLDNVDVAHATTRGVMVVNAPQSNILSAA
                     EQTMALLLANARNIPQAHNALVQGRWERSKWEGVELADKTLAVIGLGRIGKLVAQRAM
                     AFGMRIIAHDPFIAPDRARAMNIELVSLDEVALQADFLTLHVAKTPETIGLIGAEFLA
                     KAKPELRVINVSRGGIVDEAALAHAIETGQIAGAALDVFEKEPTTESPLFALPSVVVA
                     PHLGASTREAQDKAGDTIAEQVGLALAGEFVPFAVNVAAAEASETVRPYLGVAEQLGR
                     IFGGLGDGLPATIDIEYQGELAGYDTRILTLSVLKGIFETVSDDPVSYVNAPQLATDK
                     GIEIKDSSTSSSVDWINLVSISGGGHQIAGTLVGLKAEARVVQIDGHSLDVPPSANLL
                     VVRNDDRPGVIGRVGTILGDAGVNIADMDVGRRPTTDGALMVIATSQPAPDEVVEALR
                     ACDGIIEVAVIAGT"
     gene            2024..2182
                     /locus_tag="VNQ33_05360"
     CDS             2024..2182
                     /locus_tag="VNQ33_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97566.1"
                     /translation="MYDADEEPEEKPAYDWDAWEKNRFTGFNRWWFILMAAMTIALTF
                     QFFQTYGW"
BASE COUNT          321 a          735 c          806 g          324 t
ORIGIN      
        1 caacgggttc acgttctcga tcacgctcgg ggtgatgagc ggcacgacct ggatcccggt
       61 cagcgccgaa cgccgcaagg gggcgggcat ctccgcgggc gagacggtcg aggtggcgat
      121 ggtcgtggac gatgcgccga aggtcatcga ggttcccggc gacctggccg cagccctcga
      181 gcacgccggc gcccgggccg ccttcgacgc gctggcgccg agccaccgca aggagcacgt
      241 ccgggccgtc accgaggcca agaagcccga gacccgcgag cgccgcatcg ccaagtgcgt
      301 cgagatggtc cgcccctgag ccgagcagcg accgcacggt cccggacctg ggctacggcc
      361 cgatgcacct catctggcgc agctccgacc cgctcaggtg ccggcgatga cggcgacctc
      421 gatgatgccg tcgcacgcac gcagggcctc gaccacctcg tcgggggcgg gctggctggt
      481 ggcgatgacc atgagggcgc catcggtggt gggccgacgg cccacgtcca tgtcggcgat
      541 gttcacgccg gcatcgccca ggatcgtgcc gacccggccg atgacgccgg ggcggtcgtc
      601 gttgcggacc acgagcaggt tcgccgaggg cgggacgtcg agcgagtgcc cgtcgatctg
      661 caccacgcgg gcctcggcct tgaggccgac gagcgtgccg gcgatctggt ggccgccgcc
      721 gctgatggag acgaggttga tccagtcgac gctgctcgag gtggacgagt ccttgatctc
      781 gatgcccttg tcggtggcca gctgcggggc gttcacgtac gagaccgggt cgtcgctcac
      841 cgtctcgaag atgcccttca ggaccgagag ggtcaagatg cgggtgtcgt agccggccag
      901 ctcgccctgg tactcgatgt cgatggtggc gggcagccca tcgcccaggc cgccgaagat
      961 gcgcccgagc tgctcggcga cgccgaggta cggacggacg gtctcgctgg cctcggcggc
     1021 ggcgacgttc accgcgaacg gcacgaactc gccggccagc gccagcccga cctgctcggc
     1081 gatggtgtcg ccggccttgt cctgggcctc acgggtgctg gcgcccaggt gcggggcgac
     1141 caccaccgac ggcagcgcga agagcggcga ctcggtggtg ggctccttct cgaacacgtc
     1201 gagggcggca cccgcgatct ggccggtctc gatggcgtgg gccagagcag cctcgtcgac
     1261 gatgccgccg cgcgacacgt tgatgacccg cagctcgggc ttggccttgg ccaggaactc
     1321 ggcgccgatg aggccgatgg tctcgggggt cttggcgacg tggagcgtga ggaagtcggc
     1381 ctggagggcc acctcgtcga ggctgaccag ctcgatgttc atggcccggg cccggtcagg
     1441 ggcgatgaac gggtcgtggg cgatgatccg catgccgaag gccatcgccc gctgggcgac
     1501 gagcttgccg atgcggccga ggccgatgac ggcgagggtc ttgtcggcga gctccacgcc
     1561 ctcccacttc gaccgctccc accggccctg cacgagggcg ttgtgcgcct gcgggatgtt
     1621 gcgggcgttg gccagcagca gggccatcgt ctgctcggcg gccgagagga tgttcgactg
     1681 cggagcgttc acgaccatga cgccgcgggt ggtggcgtgg gcgacgtcga cgttgtcgag
     1741 gccgatgccc gctcgaccga cgaccacgag gtcggtgccc gcgtcgagca cctccttggt
     1801 gacggtggtg gcggagcgga tgatcagggc gtgcgccccc ttgatcgcgt cgaccagctc
     1861 ctcgggcgac aggcccagct gcacgtccac gtcgtggccc gccgcacgca gtgcgtcgag
     1921 gcctccgtca gcgatcttct ccgaaaccag cacacgagcc ataggggtcc gagtctgccg
     1981 ggtcgcccgg cgaagccgcc aatgcccgcg tagcctcggc cgggtgtacg acgccgacga
     2041 ggagccggag gagaagccgg cctacgactg ggacgcgtgg gagaagaacc gcttcaccgg
     2101 cttcaaccgc tggtggttca tcctcatggc ggcgatgacc atcgccttga cgttccagtt
     2161 cttccagacg tacggctggt gagcga
//
LOCUS       DATMFS010000293         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_313160, whole genome shotgun sequence.
ACCESSION   DATMFS010000293 DATMFS010000000
VERSION     DATMFS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313160"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VNQ33_05365"
     CDS             <1..377
                     /locus_tag="VNQ33_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97567.1"
                     /translation="AMDLLWRLAADCPSVVIEANFRSGSAFERERLLGLSERPVEVFC
                     RIPMEVARTRFEHRAATAERHPVHVARSLAPEVWEEFREPFGLGPMIEVDTIGPVDLD
                     TLVVEVRAALAAEAGPNRDGRP"
     gene            388..1140
                     /gene="map"
                     /locus_tag="VNQ33_05370"
     CDS             388..1140
                     /gene="map"
                     /locus_tag="VNQ33_05370"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWJ97568.1"
                     /translation="MTIDDADDLAGLSAAGRVVADTRDAMLAAVEPGITTGELDAIGR
                     AHLRAAGARSAPRLAYGFPGSTCISVNEEAAHGVPSKLRPLRNGDLVNIDVSAELDGY
                     WADTGASLAVGTVSDRAEELLTATRLAQADAMAAAVAGRPLHRIGRAVEHRARRHGFT
                     ILADLLGHGVGRSIHEDPNVSSIEDRSDPTVLWEGLVLAIEPFLAMGAMHTTTADDGW
                     TLLTNDGGLAAQFEHTVVITDGHPLVLTASAA"
     gene            complement(1175..2248)
                     /locus_tag="VNQ33_05375"
     CDS             complement(1175..2248)
                     /locus_tag="VNQ33_05375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HWJ97569.1"
                     /translation="MTAVPEVLPFGTLARADVTGRRDVDLVVLESSALRVSVLTYGAA
                     LWSVEAPDRSGQRAPVALHLPTLADMEDRARNPYLGATCGRFAGRIAGARFVLDDTLV
                     VLPPNEGAHQLHGGPDGFDRRSWGVAETAPTDDGGRVVLALTSPDGDQGFPGTLDVTA
                     VYELHGHVLRITYEAATDATTVVNLTNHAYWNLGGDDHASASGSIGDHELRLPGERYL
                     PVDGETIPLGGLAAVAGTAFDLRHERLLADVLAELPDGIDHAYEVHTADDVERAAFDG
                     LRLAAELHHPASGRTLTVATDQPAVVVYTGNRLGPPFAAQAAICLETQGYPDVPNRPG
                     LGSAVLRHGEHHRRSTELTFGVR"
     gene            2381..2728
                     /locus_tag="VNQ33_05380"
     CDS             2381..2728
                     /locus_tag="VNQ33_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWJ97570.1"
                     /translation="MPTYAAIIYSADVDYTEPQYAETTKGYMAFGEAAAAVIRGGAGL
                     YPTSTATTVKVTGGKGGDVVTSDGPYIESKDALTGFYLLECADLDEAVRYAAMIPGAW
                     AGSVEIRPILPMG"
     gene            2725..3969
                     /locus_tag="VNQ33_05385"
     CDS             2725..3969
                     /locus_tag="VNQ33_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ97571.1"
                     /translation="MTAPSGADAAGSLDRLVRTEGRAVLATLVRTTGRLDLAEDAVQE
                     AVLRALETWPRDGVPDHPRAWLTTVARNRAVDIVRREARRDHKEEEAVRMLDDDGSSD
                     WADAQDEHDLLRLVFTCCHPTLSLDAQVALSLRTLCGLTTPEVATALLVPEATMTKRL
                     TRARKKIAVANISYRVPDDHELPDRLAGVAATVYLVFNAGYSEPPVAPPGRDAAVEAL
                     ELAQTLHGLMPDEPSVIGLLALVLLQQARRGGRFDADGVAIPLAHQDRSGWDRAMIEQ
                     GVVLVGDGLRRTPDRADPYVVQAAVAACHALAPTWAATDWEAVVSWYDVLLAVHDTPV
                     VRLNRAAALAERDGPALGLEAVDAVAGLEAYPFWCASRAELLARLGRDEEARAAWAAA
                     RRLGLPEANDRWVERRMDALGR"
     gene            complement(4038..4709)
                     /gene="ung"
                     /locus_tag="VNQ33_05390"
     CDS             complement(4038..4709)
                     /gene="ung"
                     /locus_tag="VNQ33_05390"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWJ97572.1"
                     /translation="MAPQTDWNPILRGEFAKPYWPELQSFVADERRHHAVYPPPEQVF
                     AALHQTPYADVKAVILGQDPYHGAGQAHGLCFSVRPGVPLPPSLRNIFQELEADVGVR
                     PPNHGCLDAWADQGVLLLNTTLTVRAHRAASHQGHGWETFTDEVIRAVDAKDERVVFI
                     LWGAAARKKKALIDRSRHAVIESAHPSPLSAHNGFFGSRPFSRTNAALDAAGRAPIDW
                     SIPDR"
     gene            4832..6154
                     /locus_tag="VNQ33_05395"
     CDS             4832..6154
                     /locus_tag="VNQ33_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ97573.1"
                     /translation="MLAFGGLAAIVSTLLAIVCFEVTRSTLIQQRERVAERQTYVNAR
                     SVDNALTRDPASPTAALADAQTSRQGFALVRVDGTWYSSSVTGDRGDVPGSLISVIDT
                     GAAARQRVHLDSGPAVAVGVPLSGASTGPVYVELVPMSEVSDAVNRIRQGLILAAAVT
                     TLAGVIAGRWASARILRPVSRTAEAANLISEGALDRRLESDGDADLEPLVQSFNNMVD
                     GLQSRIEREARFASDVSHELRTPLAAMLSALSVARRRVKEPAATEALDHLQHEVDRFT
                     TLITDLLEISRVEAGVAELVLEEVEPEAFVAEVLVSMDRQAVPVVVERDAPARIRLDS
                     RRMGQILVNLVQNADNYGGGATEIRLGGDSGHLRVIVDDAGPGIPPVQRELVFERFVR
                     GDDPSVPGTGLGLALVKEHVRLHEGSVVIKRSPSGGARFVVTIPRTLR"
     gene            6151..>6507
                     /locus_tag="VNQ33_05400"
     CDS             6151..>6507
                     /locus_tag="VNQ33_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97574.1"
                     /translation="MSRALRTAGAALLVALVASWALAACGVSPQGAATRIEPEDVPFG
                     LLEDQPTTTVVAAGKTAAIYLLSEDRLIPVDRSVPAGADLGDLLRQAMAGPTEVEQSL
                     GITSAVPAGAVSSVDAS"
BASE COUNT          846 a         2300 c         2467 g          894 t
ORIGIN      
        1 cggccatgga tctgctgtgg cggctggcgg cggactgccc gtcggtcgtg atcgaggcca
       61 acttccgatc ggggagcgcc ttcgagcgcg agcggctgct cgggctgtcg gagcggcccg
      121 tcgaggtgtt ctgcaggatc ccgatggagg tggcccgcac tcggttcgag caccgggcgg
      181 ccaccgccga gcgccaccca gtccacgtgg cccgttcgct cgcgcccgag gtgtgggagg
      241 agttccggga gccgttcgga ctcggaccga tgatcgaggt cgacaccatc ggaccggtgg
      301 atctcgacac gctcgtggtc gaggtgcggg ccgcgctggc agctgaggcc ggcccgaatc
      361 gggacggccg gccgtagcgt ttggggcatg acgatcgatg atgcggacga cctggcggga
      421 ctgagcgcgg ccggacgggt ggtggccgac acgcgcgacg cgatgctcgc tgccgtcgag
      481 cccggcatca ccacgggtga gctcgacgcc atcggccggg cccacctgcg agcggctggc
      541 gcccgatcgg cgcctcgcct tgcctacggc ttcccgggga gcacgtgcat cagcgtgaac
      601 gaggaggcgg cgcacggcgt gccgtccaag ctgcggccgc tgcgcaacgg cgacctggtc
      661 aacatcgacg tgtcggccga gctcgacggc tactgggccg acaccggcgc cagcctggcg
      721 gtcggcacgg tcagcgaccg ggccgaggag ctcctcacgg ccacccgctt ggctcaggcc
      781 gacgccatgg ccgcggcggt ggccggccgt ccgctccacc gcatcggccg tgcggtggag
      841 catcgggccc gacggcatgg cttcacgatc ctggccgacc tgttgggcca cggcgtcgga
      901 cgctcgatcc acgaggaccc gaacgtgtcg agcatcgagg atcggtccga ccccacggtc
      961 ctatgggagg gcctggtgct cgccatcgag ccgttcctgg ccatgggtgc catgcacacc
     1021 acgacggctg acgacggctg gacgctgctc accaacgacg gcggtctggc tgcgcagttc
     1081 gagcacacgg tcgtgatcac cgacggccac ccgctcgtcc tgactgcctc ggccgcctga
     1141 ccgcggccgc ctccgagccg gccacgggcg agcgtcagcg gacgccgaag gtgagctcgg
     1201 tgctgcggcg gtggtgttcg ccgtggcgga gcacggccga gccgaggccg ggacggttcg
     1261 ggacgtcggg gtagccctgc gtctcgaggc agatggcggc ctgggcggcg aaggggggac
     1321 cgagccggtt gccggtgtag acgacgacgg cgggctggtc ggtggcgacg gtgagcgtgc
     1381 ggccggaggc ggggtggtgg agctcggcgg cgaggcggag gccgtcgaac gcggcgcgct
     1441 cgacgtcgtc ggcggtgtgc acctcgtagg cgtggtcgat gccgtcgggc agctcggcga
     1501 gcacatcggc cagcaggcgc tcgtggcgca ggtcgaaggc ggtgccggcg acggccgcga
     1561 ggccgccgag cgggatggtc tcaccgtcga ccgggaggta gcgctcgccg ggcaggcgca
     1621 gctcgtggtc gccgatcgag ccagaggccg atgcgtggtc gtcgccgccg aggttccagt
     1681 aggcgtggtt cgtgaggttc acgacggtgg tcgcgtcggt ggcggcctcg taggtgatcc
     1741 gcagcacgtg gccgtggagc tcgtagacgg ccgtcacgtc gagcgtgccc gggaagccct
     1801 ggtcgccgtc gggactggtg agggcgagga ccacgcggcc cccgtcgtcg gtcggcgcgg
     1861 tctcggccac gccccagctg cggcggtcga agccgtcggg cccgccgtgg agctggtggg
     1921 cgccctcgtt gggcgggagc accacgagcg tgtcgtcgag gacgaagcgg gcgccggcga
     1981 tgcgcccggc gaagcgtccg cacgtagcgc cgaggtacgg gttgcgggcc cggtcctcca
     2041 tgtcggcgag cgtcggcagg tgcagggcga cgggagcgcg ttggccggag cggtcaggtg
     2101 cctcgacgct ccacagcgcc gccccgtagg tgaggaccga cactcggagg gcagagctct
     2161 cgagcaccac gaggtcgacg tcgcgccggc cggtcacgtc ggcacgggcg agcgtgccga
     2221 acgggaggac ctccgggacc gcggtcatcg tccgaacgta ccggccgctt gcggacggcg
     2281 ccgggagaat ctgtggtcga ctgtcctttt cggctcggcc ggttcgtctc ctctgcgacg
     2341 accggccact cgggccggcg catccgaaag gaccaccacc atgcccacct acgccgccat
     2401 catctactcc gccgacgtcg actacaccga gccgcagtac gccgagacca cgaagggcta
     2461 catggcgttc ggcgaggcgg ccgccgccgt catccgcggc ggcgccggcc tctaccccac
     2521 gagcaccgcc accacggtga aggtcacggg gggcaagggc ggcgacgtcg tcaccagcga
     2581 cggcccgtac atcgagtcca aggacgcgct gaccggcttc tacctgctgg agtgcgccga
     2641 cctcgacgag gcggtccgct acgccgccat gatcccgggc gcctgggcgg gcagcgtgga
     2701 gatccggccg atcctcccga tggggtgacc gctccgtcgg gcgccgacgc cgcgggctcg
     2761 ctcgaccggc tggtgcggac cgagggacga gcggtgctcg ccaccctcgt ccgcaccacc
     2821 gggcgcctcg acctggccga ggacgccgtg caggaggccg tgctccgcgc cctcgagacc
     2881 tggccgcgcg acggcgtgcc cgaccacccg cgcgcctggc tcacgacggt ggcccgcaac
     2941 cgggcggtcg acatcgtgcg gcgcgaggcc cggcgcgacc acaaggagga ggaggccgtc
     3001 cgcatgctcg acgacgacgg ctccagcgat tgggcggatg cccaggacga gcacgacctg
     3061 ttgcgcctgg tgttcacctg ctgccacccg acgctgtcgc tcgatgccca ggtggcgctg
     3121 agcctccgca ccctgtgcgg tctcacgacg cctgaggtgg cgacggcgct gctcgtgccc
     3181 gaggcgacga tgaccaagcg cctcacccgg gcccgcaaga agatcgccgt cgccaacatc
     3241 tcgtaccggg tgccggacga ccacgagctg ccggaccgcc tcgccggcgt ggccgccacc
     3301 gtgtacctcg tgttcaacgc ggggtacagc gagccgccgg tcgcgccgcc gggccgtgac
     3361 gccgcggtcg aggcgctcga gctggcccag acgctgcacg gcctgatgcc cgacgagccg
     3421 tcggtcatcg gcctgctcgc cctggtgctg ctgcagcagg cgcgtcgcgg gggccgcttc
     3481 gacgcggacg gcgtcgccat cccgctggcc caccaggacc gttcgggctg ggaccgagcg
     3541 atgatcgagc agggcgtggt gctggtgggc gacggcctgc ggcggacgcc ggaccgggcc
     3601 gatccctacg tggtgcaggc ggccgtcgcg gcctgccacg cgctggcccc gacgtgggcg
     3661 gccacggatt gggaggcggt ggtgtcctgg tacgacgtgc tgctcgcggt gcacgacacc
     3721 ccggtggtgc ggctgaaccg ggcggccgcg cttgccgagc gcgacgggcc ggcgctcggg
     3781 ttggaggcgg tcgacgccgt cgcgggcctg gaggcgtacc cgttctggtg cgccagccgg
     3841 gccgagctcc tcgcccgcct cggtcgggac gaggaggccc gcgccgcgtg ggccgcggcg
     3901 cggcggctgg gcctgcccga ggccaacgac cggtgggtcg agcggcggat ggacgccctc
     3961 ggccggtgac aggccgcgtc ggtgacgggc cgcgtcgatc cggtcctgcg acgaccgcct
     4021 gccgtcggac cttgccctca gcggtcgggg atcgaccagt cgatgggggc gcgcccggcg
     4081 gcgtcgagcg ccgcgttggt gcgggagaac gggcggctgc cgaagaaccc gttgtgcgcc
     4141 gacagcggcg aggggtgggc cgactcgatc accgcgtggc ggctgcggtc gatcaacgcc
     4201 ttcttcttcc gggcggcggc gccccagagg atgaacacga cgcgctcgtc tttggcatcg
     4261 accgcccgga tcacctcgtc ggtgaacgtc tcccagccgt gcccttggtg cgaggccgct
     4321 cggtgggctc gcaccgtgag cgtcgtgttg aggagcagga cgccctggtc ggcccaggca
     4381 tcgaggcagc cgtggttcgg tgggcgcacg ccgacgtccg cctcgagctc ctggaagatg
     4441 ttgcggagcg aaggcgggag cggcacgccg ggtcggacgg agaagcacag gccgtgggcc
     4501 tggcctgccc cgtggtacgg gtcctgcccg aggatcaccg ccttcacgtc ggcgtacggc
     4561 gtctggtgca aggcggcgaa cacctgctcc ggcggtgggt acaccgcgtg gtgccggcgc
     4621 tcgtcggcca cgaacgactg gagctcgggc cagtagggct tggcgaactc tccgcgcagg
     4681 atggggttcc agtccgtctg cggggccacg gcgcttgacc ctacccgtgc gggagtgacc
     4741 ccgatcgggc cgccgcgcct gccacagtcg aggggtgcaa gggagcggag gtgcagcacg
     4801 gacctggcga ccgccgctgc gcacgcgcgc catgctggcc ttcggcggac tggccgcgat
     4861 cgtctccacg ctgctcgcca tcgtctgctt cgaggtgacc cggtccaccc tgatccaaca
     4921 gcgcgagcgc gtcgccgaac gccagaccta cgtgaacgcc cgctcggtcg acaacgccct
     4981 cacgcgggat ccggcatcgc cgacggcggc cctggccgat gcccagacca gccgccaggg
     5041 cttcgccctg gtccgggtcg acggcacctg gtactcgagc tcggtcaccg gtgaccgcgg
     5101 cgacgtgccc ggctcgctga tctccgtgat cgacacgggt gccgcagccc gccagcgcgt
     5161 ccacctggac tccgggcctg cggtcgcggt gggggtgccg ctgtcgggcg cctcgacggg
     5221 tccggtgtac gtcgagctcg tgcccatgtc cgaggtctcc gatgccgtca accgcatccg
     5281 tcagggcctg atcctcgcgg ccgcggtcac gaccctggcc ggcgtgatcg ccgggcggtg
     5341 ggcgagcgcc cgcatcctgc gcccggtcag ccgcacggcc gaggccgcca acctcatcag
     5401 cgagggtgcg ctcgaccgcc gcctcgagag cgacggtgac gccgacctgg aaccgctggt
     5461 ccagtcgttc aacaacatgg tcgacggcct gcagagccgc atcgagcgcg aggcgcgctt
     5521 cgcgtccgac gtgagccacg agctccggac gccgctcgcc gccatgctct ccgccctctc
     5581 cgtggcccgc cggcgggtga aggaacccgc ggccaccgag gcgctcgacc acctccagca
     5641 cgaggtcgac cgcttcacca ccctcatcac cgacctgctc gagatctccc gggtggaagc
     5701 cggggtcgcc gagctggtcc tcgaggaggt cgagccggag gccttcgtcg ccgaggtcct
     5761 ggtgtccatg gaccggcagg cggttcccgt ggtcgtcgaa cgggatgcac cggctcggat
     5821 ccgtctggac tcgcgccgca tggggcagat cctggtgaac ctggtgcaga acgccgacaa
     5881 ctacggcggc ggcgccaccg agatccgcct cggtggcgac tccgggcacc tccgggtgat
     5941 cgtggatgat gccggtcccg gcattccgcc ggtccagcgc gagctggtgt tcgagcggtt
     6001 cgtgcgcggc gacgacccgt cggtcccggg aaccgggctg gggctcgcgc tcgtcaagga
     6061 gcacgtgcgc ctgcacgagg gctcggtcgt gatcaagcgg tcaccgtctg gcggcgcgcg
     6121 cttcgtcgtc accatcccga ggacgttgcg gtgagccgcg ccctgcgtac ggcgggcgcc
     6181 gcgctgctgg tcgcgctcgt cgcctcgtgg gcgctggccg cgtgcggcgt gtcgccgcag
     6241 ggggcggcaa cccgcatcga acccgaagac gtgccgttcg ggctcctcga ggaccagccg
     6301 acgaccacgg tggtggcagc gggcaagacc gccgccatct acctcctcag cgaggaccgg
     6361 ctgatccccg tcgaccgctc ggtgccggcc ggcgccgacc tcggagacct cctccgccag
     6421 gccatggccg ggccgaccga ggtggagcag tccctcggca tcaccagtgc cgtcccggcg
     6481 ggtgcggtgt catccgtcga tgccagc
//
LOCUS       DATMFS010000294         6278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_34253, whole genome shotgun sequence.
ACCESSION   DATMFS010000294 DATMFS010000000
VERSION     DATMFS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34253"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VNQ33_05405"
     CDS             <1..522
                     /locus_tag="VNQ33_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97575.1"
                     /translation="SVPLGKAGVGSAMNDTTRELGGALGVAVLGSVVTSQYTSSLGPA
                     ITSLPAQAKAMADSGLAGALQVASKLPGSAGASLADAAKQSFVDGLAAAAIVATIVVI
                     SAAVAAWFLLPSHFGMQATSPGGPATDDLGDDRSLEGTLDIDHGTPIDADGPSEGAAA
                     ADEGEREPSPVLD"
     gene            complement(767..1735)
                     /locus_tag="VNQ33_05410"
     CDS             complement(767..1735)
                     /locus_tag="VNQ33_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWJ97576.1"
                     /translation="MPAVTVDDILTLPRLAEPDPATATDRKVMSVTTGPKGFEGEGFP
                     VTRAFAGVDLAELDPFIHMDQMGEVEYAAGEPKGTPWHPHRGFETVTYIIDGTFLHQD
                     SHGGGGVITNGATQWMTAGSGLLHIETPPESLVESGGLFHGIQLWVNLPAAQKWATPN
                     YQNLEGADVTLVTSPDGGALIRVIAGDVGGHRGPGSTKSPITLLHLTIEPGAQVRLPW
                     PEHFNGLVYGLSGAGTVGANQRPLGTGQLAVTAPGDVLTIGGSPSPDSRTDAFDVLVL
                     GGQKIGEPIAWQGPFVMNTREELIETFRDYEAGKLGVIPPNALGIR"
     gene            complement(1974..2825)
                     /locus_tag="VNQ33_05415"
     CDS             complement(1974..2825)
                     /locus_tag="VNQ33_05415"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HWJ97577.1"
                     /translation="MSTLPEVIVEDDADTWDEARDILLALARERRGSGSDVGDRDLDM
                     LVAGRKWIYRGMRDSTWGLRSSFHRFWGSMSQVARARLHQDQLEELRRAIGEHLAPTL
                     SLEDPQDPELEAFGQHYDMPTLLLDWSRNPLVAAFFACADSARNFNNTSLRLVVCALR
                     TDSPVWNDQQGCVQIVQAKNPINERVVRQMGLFTYDRSGKATIEEYVRSALDDEQVVQ
                     DVTTRGPVIRKVTFPLGVVAEALEELEVFSINSYAMFPDHTGVVRQAQLRIIMRQLRK
                     GAATPSV"
     gene            complement(2822..4393)
                     /locus_tag="VNQ33_05420"
     CDS             complement(2822..4393)
                     /locus_tag="VNQ33_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97578.1"
                     /translation="MASLDPTGLPFGSPEGENTSLSAALDLGDSLKLPDGGLKVRAFV
                     GWKGTGKTLYLKLHHEAAQARAHRDGIYVEPDYSYRQPSSDAIIEFSARSSQETLVDR
                     WQTAWKRSAILAAASHLAYARELRDHNDIEESSPPHFRRLLRLCGGAIGIYDAFTVLN
                     EHDEVTRGRGPLDSAEWNKSLLNIARVASNSPPISLYADMMDAEYEIAPSYWMKCQLG
                     LLKAIASLLEDPQFGGRVHVTVTLRDHTLALLGASEHQTKMLFQAYLKTLEWDYTKLK
                     ALLFTRIGSLSSEYLTYPGLASSDPMRAWLGFDRVENEVRGVSESAVGYIIRHTRLVP
                     RDLVVLGNAISMRVASAGPGEFTALNFKQTISDCARRFGAEQIAVCAQQIAADSLPSE
                     AIDGSLGSTYWGSAGFRQNLASLIERTVAANRVDRFGVEGLERMRSVIRVEHLSADLV
                     FSAMWVNRLLGIVETDPVDGVARCRFFGSSTSTSILLPNTADEYVLHPCLIDTVPSVA
                     GKGSDPVILGRGWDE"
     gene            complement(4556..6061)
                     /locus_tag="VNQ33_05425"
     CDS             complement(4556..6061)
                     /locus_tag="VNQ33_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWJ97579.1"
                     /translation="MAERPKRQRTSTSNFGVGKRENHDASGFYERFGAPVISSDDTVN
                     VAPSTDQIWVHDARDMHELPAASVALVVTSPPYFAGKAYEEELGEGSIPASYVDYLEM
                     LTDVFAECARVLEPGGRIAVNVANLGRKPYRSLSADVTHILQDRLGLLLRGEIIWQKA
                     RGAGGNCAWGSFQSAKNPVLRDLSERVIVASKGRFDRALSTAKREVAGLPARSTIGKD
                     KFLDLTLDMWEFPPESATRVGHPAPFPVELPERLIELYTFEDDLVLDPFMGSGSTGVA
                     AVQTRRRFAGYDTDADYAAAAMERIDQARQDHDWAQKQRENAYAPLLTSVFPKARRAE
                     ADEMVSFQRRATLDGTRAQEVAEFLLRQCGFGDELQEKVKFPVGVEANFVGIDATGCE
                     WIFDVSGAFSSSRPGLERTDTLWKALGKAAILRTQHPDKERPSRYVLLTTDMPKKGSA
                     GYKALEAAQKDGLIWDVLLLDQPGTVAELLHYASGKCSAVEARRGSITASV"
BASE COUNT         1074 a         1939 c         2122 g         1143 t
ORIGIN      
        1 tcggtgccgc tcggcaaggc gggcgtcggc tcggccatga acgacaccac ccgagagctc
       61 ggcggtgcgc tcggcgtggc cgtcctcggc tcggtcgtga cgtcgcagta caccagcagc
      121 ctgggcccgg ccatcaccag cctccctgct caggccaaag ccatggccga ctccggtctc
      181 gctggggccc tgcaggtcgc cagcaagctc cccggttcgg ccggtgcctc gctcgccgat
      241 gccgccaagc agtccttcgt ggacggtctg gcggcggcgg ccatcgtcgc caccatcgtg
      301 gtgatctcgg cggccgtcgc ggcctggttc ctcctgccgt cgcacttcgg gatgcaggcc
      361 accagccccg gcggccccgc caccgatgac ctcggtgacg accgttcgct cgagggcacc
      421 ctcgacatcg accacggcac ccccatcgat gccgacggtc cgtccgaggg cgccgccgcc
      481 gctgacgagg gcgagcgtga gccgtccccg gttctcgatt gaccgactga tcacgcctcc
      541 aagcagaagc gccggcccca ctggggccgg cgcttccgcg ttgtgggtgc tgttgtgctg
      601 cacctcgctc gaatttcgta tgtcagaccg cccagacagg gtctctcgca tacgaagttc
      661 gtcccacgcc ccgggccctc cctgaacttc gtatgccggg acgcccaaac gggcgctctc
      721 ccatacgaag ttcgactgcg gggcaggagg agggggaggg ggagggtcag cggatgccga
      781 gggcgttcgg ggggatgacg ccgagcttgc cggcctcgta gtcccggaag gtctcgatca
      841 gctcctcgcg ggtgttcatc acgaacgggc cctgccaggc gatgggctcg ccgatcttct
      901 ggccgccgag gacgagcacg tcgaaggcgt cggtccggga gtccgggctg ggcgagccgc
      961 cgatggtgag cacgtcgccg ggggcagtga cggcgagctg gccggtgccg agcgggcgct
     1021 ggttcgcccc gacggtgccg gcgccggaga ggccgtagac caagccgttg aagtgctcgg
     1081 gccacgggag ccggacctgg gcgccgggct cgatggtcag gtggagcagg gtgatgggcg
     1141 acttggtgct gcccgggcca cggtgaccac cgacgtcgcc ggcgatcacc cggatgagcg
     1201 caccgccgtc gggcgaggtg accagcgtga cgtcggcgcc ctcgaggttc tggtagttcg
     1261 gcgtcgccca cttctgggcg gcaggcaggt tcacccacag ctggatgccg tggaacaggc
     1321 cgcccgattc cacgagggac tcgggtggcg tctcgatgtg gaggaggccg ctgcccgcgg
     1381 tcatccactg ggtggcaccg ttggtgatga cgccgccacc gccgtgggag tcctggtgca
     1441 ggaacgtgcc gtcgatgatg taggtgacgg tctcgaaccc gcggtgcggg tgccacggcg
     1501 tgcccttggg ctcgccggcg gcgtactcga cctcacccat ctggtccatg tggatgaacg
     1561 gatcgagctc ggcgaggtcc accccggcga acgcccgggt gacggggaag ccttcgccct
     1621 cgaagccctt gggaccggtg gtgacgctca tgaccttgcg gtcggtcgcg gtggccggat
     1681 cgggctcggc gaggcggggg agggtgagga tgtcgtcgac ggtgactgca ggcatggtgt
     1741 cgatcccttc gatcgatcgg ccgcccccgg ttggggcctc gccggttccc ttgaacaaac
     1801 atagttgaca tgtcaactat tcctgtgtcg cgagctctcg gccgatctac tccgttgacc
     1861 agagcgcctg catggcgcgc aagaggtcga tgctgcgatt cccgtgctgt cctctgcccc
     1921 ctgtgccggg tcggccgaga gcgtctggag tcggaggccg gacgggatcg cggtcagacg
     1981 gatggggtgg cagcgccctt gcgaagctgg cgcatgatga ttcgcaactg ggcctgtcgg
     2041 acgacgcctg tgtgatccgg aaacatcgcg tagctgttga tggagaagac ttcgagctcc
     2101 tcgagcgcct ctgcaacgac gccgaggggg aaggtcacct tccggatgac cgggcctcgt
     2161 gttgtgacgt cctgcacgac ctgttcgtcg tccagtgcac tccgcacgta ctcctcgatc
     2221 gttgctttgc ccgatcggtc gtaggtgaac agacccattt gccgaacgac gcgctcgttg
     2281 atcggattct tggcctgaac tatctggacg cagccctgtt ggtcattcca gacaggtgag
     2341 tcagtgcgaa gtgcacaaac cacaagccgg agtgaggtgt tgttgaagtt ccgggcagag
     2401 tcagcgcacg cgaaaaacgc ggcgacgagt gggttgcggg accaatcgag cagcagtgtc
     2461 ggcatgtcgt agtgctgtcc gaatgcctcg agttcggggt cttgaggatc ttcgagggag
     2521 agcgtcgggg cgaggtgctc gccgattgct cgtcgaagct cctcgagctg gtcctggtgt
     2581 aagcgcgccc gggcgacctg actcatggac ccccagaatc gatggaacga gctccgcagg
     2641 ccccaggtcg agtcgcgcat tcctcggtag atccacttac gccccgcaac aagcatgtcg
     2701 agatccctgt caccgacatc tgacccggaa cctcggcgct cgcgtgccag tgccaacaga
     2761 atatctcgcg cctcatccca ggtatctgca tcgtcttcga cgatcacctc tggaagcgtg
     2821 ctcattcatc ccatccgcgg ccgaggatca caggatcaga acctttcccc gccactgacg
     2881 ggaccgtatc aatcaagcaa gggtgaagga catactcgtc cgcagtatta ggcaggagaa
     2941 tcgaggtgga ggtcgaggac ccgaagaagc gacaacgggc aacgccatcc accgggtcag
     3001 tttcgacgat accaagaagt cgattgaccc acatcgcgga gaagacgagg tctgcagaca
     3061 gatgttcgac ccggatgacg ctccgcatac gctccaaacc ctccaccccg aatcggtcta
     3121 ctcggttggc ggccaccgtc cgttcaatga ggctagccag attctggcga aacccagccg
     3181 agccccagta ggtggaaccg agtgagccgt ctatcgcctc ggatgggagc gagtctgcgg
     3241 cgatctgttg agcacagact gcaatctgct ctgcaccgaa tcgtcgagca caatcactta
     3301 tcgtttgttt gaagttcaag gcggtgaact ctccgggccc agcggaggcg actcgcatgc
     3361 tgatggcgtt tccaagaacc acaaggtcgc gcggtacgag tcgcgtatgt cggatgatat
     3421 atccgacggc tgattctgag acgcctcgaa cctcgttttc gacccggtcg aatccgagcc
     3481 aagccctcat cgggtctgac gaggccaagc ccggatacgt taggtactcg cttgacagag
     3541 agccgatccg cgtgaacaga agcgccttga gcttcgtgta atcccattcg agcgtcttga
     3601 ggtatgcctg gaacagcatt tttgtctgat gctcgctggc ccccaaaagg gccaacgtgt
     3661 ggtcacgaag ggtaacggtg acgtggacgc gtccgccgaa ctgaggatct tcaaggagcg
     3721 acgcgatggc cttaagaaga ccaagttgac acttcatcca atacgagggc gcgatctcat
     3781 actcggcatc catcatgtct gcgtatagtg aaattggtgg tgagttgctc gcaaccctgg
     3841 cgatgttgag caagctcttg ttccattccg ccgaatcgag agggccgcgt ccgcgggtga
     3901 cctcgtcgtg ttcattcaag actgtgaatg catcatatat tccgatagcg ccgccacaaa
     3961 gacggagcag acgccggaaa tggggaggtg aggactcttc gatatcattg tgatctcgaa
     4021 gttcgcgcgc gtatgcaagg tgagatgcag cagccagaat ggcggatcgc ttccaagcgg
     4081 tctgccatcg atccaccaga gtttcctgac tggagcgagc cgagaactcg atgattgcat
     4141 cggagctcgg ttgccgatat gagtagtccg gctcgacgta gatgccgtcg cggtgtgcgc
     4201 gggcctgtgc tgcctcgtga tggagcttga ggtagagcgt cttacctgtg cccttccacc
     4261 caacgaaggc acggaccttt agcccaccgt ctgggagctt gagcgaatca cccagatcca
     4321 gtgcagcgga tagcgacgtg ttctcgcctt cggggctgcc aaacgggagt cccgtaggat
     4381 ctagggatgc catctcgccc cctgccaacg ctgcttcccc caccgtagac ccgtcaatag
     4441 ctgcgcgata tgtacatcag attaacacac ctgtctgtgg ggcgtccttg aaccgagtgt
     4501 ccgcgcgaac gacctcaagt gcagtccgtc gtagctggcc cgctgatgaa tcgggtcaga
     4561 ccgaagcagt gatcgagccg cggcgggctt ccacggccga gcatttaccc gacgcgtagt
     4621 gcagcagttc ggcgacggtg cctggctggt caagcagcag gacgtcccag atgaggccgt
     4681 ccttctgggc ggcctcaagt gccttgtacc cagcggaacc cttcttgggc atatcggtag
     4741 tgaggagcac atagcggctc ggccgttcct tgtcggggtg ttgcgtgcgg agaatcgcgg
     4801 ccttgcccag tgccttccat agcgtgtcgg tgcgttcgag gccaggccgg gagcttgaga
     4861 aggctcccga cacgtcgaag atccactcgc agccagttgc gtcgatgccg acaaagtttg
     4921 cctcgacacc gactgggaac ttcaccttct cctggagctc atcgccgaag ccgcattggc
     4981 ggagcaggaa ctcggcaacc tcctgcgcac gggtgccatc gagcgtcgcc cggcgttgga
     5041 agctcaccat ttcgtcggct tcagcgcgac gagccttcgg gaagacggac gtcagaagcg
     5101 gtgcgtaggc gttctcgcgt tgcttctgcg cccagtcatg gtcctggcgg gcctggtcga
     5161 ttcgctccat ggccgcggcc gcgtagtcgg cgtcggtgtc gtagcccgcg aagcggcgtc
     5221 gggtctggac ggcggcgacc ccggtggacc cggagcccat gaacgggtcg agcacgaggt
     5281 catcttcgaa cgtgtacagc tcgatcaggc gctcgggcag ctcgaccggg aacggagccg
     5341 ggtggccgac gcgggtggcc gactccggcg ggaactccca catgtcgagc gtgaggtcga
     5401 ggaacttgtc cttgccgatg gtgctgcggg cggggagccc ggcgacttcg cgcttggccg
     5461 ttgacagagc gcggtcgaag cggcccttgc tggcgacgat gacccgctct gacagatctc
     5521 gcaggacggg gttcttggcg ctctggaagc tgccccaagc gcagttgccg ccggcgccgc
     5581 gggccttctg ccagatgatc tcgccgcgca ggagcaggcc gaggcggtct tggaggatgt
     5641 gggtgacgtc ggccgagagc gagcggtacg gcttgcgccc gaggttggcg acgttgacgg
     5701 cgatgcgacc gcccggctcc agcacccggg cgcattcggc gaagacgtcg gtgagcatct
     5761 ccaggtagtc gacgtagctg gcggggatgg atccttcgcc gagctcctcc tcgtacgcct
     5821 tgccggcgaa gtagggcggt gaggtgacca ccagcgcgac cgacgcagcg ggtagctcgt
     5881 gcatgtcgcg cgcgtcgtgc acccagatct ggtcggttga gggagcgaca ttgaccgtgt
     5941 cgtccgagga gatgaccgga gcgccgaagc gctcgtagaa gcccgacgcg tcgtggttct
     6001 cgcgcttgcc gacgccgaag ttggaggtgc tggtcctctg ccgcttcggt cgctcagcca
     6061 tggacgaaac cctactggtg gccactgaca gcgaagaggt cctgatcgcg cgggtgttcg
     6121 gtgccggcca gctggcggcg atgctcaggc gatcgaggcg ctcagcccgc ctcgggttcg
     6181 aaccagacga cggccagggg tgggagggtg aggcgggccg acgtcgtctg gccgtgggcg
     6241 ggcgggccgc cggcggtgac ggccccgagg ttgccgac
//
LOCUS       DATMFS010000295         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80329, whole genome shotgun sequence.
ACCESSION   DATMFS010000295 DATMFS010000000
VERSION     DATMFS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80329"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2751)
                     /locus_tag="VNQ33_05430"
     CDS             complement(<1..2751)
                     /locus_tag="VNQ33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97580.1"
                     /translation="MKIDGWHIDGYGVHADHSAEGLGDGLTIVAGPNESGKTTLQHFL
                     VGMLFGFPPVTSKKEHHPPLRGGTFGGRLFITTHAGEQLTIHRSKARSSLRVTHADGA
                     PFNGDLAEVLGGSSRELFESIFSVRLENLAELKALTEDQVRDRVFSAGILGAGRSAQA
                     ALAQLGDQRDALLKPNGRGERYRIKELRSQLAEARLRLDEAQRESRALPAHLRQLELL
                     AGRAKTLGTEADRLRSELNLVTAIIELWPRWSAAADARHELDQIGTVPSLPADASARL
                     HTSIGHHRSAAEAATRAVDERTEAEAHLAGLDSPVLDLDAIDRIERLARQIDPERERA
                     RRIDELAARADQQRLELERDLALLGDGRDLAWLEARPAGASNAAGLRQAAAAVAEARN
                     EVRDAARAAEQLTTEIEVETAELERAEAALADLPALRPSDAVAGLDAATALAALTTQR
                     EVAVRRIDDARARREAALADVPEAGAAPTAAFIAAAVALVAGGAALALGQPSIVGAIV
                     TAAVVLAITGFALRARVPGPVSTADIDAVIDLATDDVRRIDADLVGPLDILRLDAVPA
                     ATAAAGIRTHAERLASAAQQADDQRRRVAAERSGHQDRAARRTTAATRRVEAARAQLQ
                     AVEATWEEWLAGHDLPLDLDPAGATEFLNTVDRAKGAARHLAHTASDLQTQRAAAVAF
                     GDEVQALVTELGDQGTPDVDPLRVLDRLATRAAGQRALLAQIADAKNTVERASEVERR
                     AGRDRAEAADQLAADLQAIGATTVDDALATLDRVAAAAKLRIVIDETDARLEAAVGGA
                     DDRRTRAVELLAAANPASWKEQAQTLRHRLGEAEADRDRCIQDRAAVQAEVDKLTTSA
                     DVPSCELRVAGLEAELVGAVTTWASLSLAHQVVEGTLAKYQRERQPDVVKRAAALFTQ
                     VTG"
BASE COUNT          326 a          953 c         1201 g          404 t
ORIGIN      
        1 gccagtgacc tgggtgaaca gggcggcggc gcgcttgacg acgtcgggct ggcgctcgcg
       61 ctggtacttg gccagcgtgc cctcgaccac ctggtgggcg agcgagagcg acgcccaggt
      121 ggtgacggcg ccgacgagct cggcctcgag gccggcgacc cggagctcgc agctgggcac
      181 gtcggccgag gtggtgagct tgtcgacctc ggcctggaca gcagcgcggt cctggatgca
      241 gcggtcccgg tcggcctcgg cctcgccgag ccggtggcgc agggtctggg cctgctcctt
      301 ccagctcgcc gggttggcgg cggcgagcag ctcgacggcc cgggtgcggc ggtcgtcggc
      361 accaccgacg gcagcctcca gccgggcatc ggtctcgtcg atgacgatcc ggagcttcgc
      421 tgcagcggcg acccggtcga gggtggcgag ggcgtcgtcg accgtcgtgg cgccgatggc
      481 ctggaggtcg gcggccagct ggtcggcggc ctcggcgcgg tcgcgcccgg cccgccgctc
      541 gacctcgctg gctcgctcga cggtgttctt ggcgtcggcg atctgggcga gcagggcccg
      601 ctggccggcg gctcgggtgg ccagtcggtc caggacccgg agggggtcga catcgggggt
      661 gccctggtca ccgagctcgg tgaccagggc ctggacctcg tcaccgaagg cgaccgccgc
      721 ggcgcgctgg gtctggaggt cggacgcggt gtgggcgagg tggcgggcgg cgcccttggc
      781 ccgatcgacg gtgttcagga actcagtggc acccgctgga tcgaggtcga gcgggaggtc
      841 gtggccggcg agccattcct cccacgtcgc ttcgaccgcc tgcagctggg cccgagccgc
      901 ctcgactcgc cgggttgccg cggtggtgcg tcgggcggcg cggtcctggt ggcccgaccg
      961 ttcggcggcg acccgcctcc gctggtcatc ggcctgctgg gccgcagatg ccagccgctc
     1021 ggcgtgggtc cggatgccgg ctgctgcggt ggcggccggg acggcgtcga ggcgcaggat
     1081 gtcgagcggg ccgacgaggt cggcgtcgat gcggcgcacg tcgtcggtgg ccaggtcgat
     1141 gacggcgtcg atgtcggcgg tggagaccgg cccggggacc cgggcacgga gggcgaagcc
     1201 ggtgatcgcc aagacgacgg cggcagtgac gatggcgccg acgatgctcg gctggccgag
     1261 ggcgagggcc gctccgccgg ccacgagggc gacggcggcg gcgatgaacg ctgcggtggg
     1321 cgcggcgccg gcctcgggca cgtcggccag ggcagcctcc cggcgggccc gggcatcgtc
     1381 gatgcggcgg acggcgacct cccgctgggt ggtgagggcg gcgagggcgg tggcggcgtc
     1441 gagcccggcc acggcgtccg acgggcggag cgcgggcagg tcggcgaggg cggcctcggc
     1501 ccgttccagc tcggcggtct cgacttcgat ctcggtggtc agctgctcgg cggcccgggc
     1561 ggcgtcgcgg acctcgttgc gggcctcggc caccgcggca gccgcctggc gcaggccggc
     1621 agcgttgctg gctccggccg ggcgggcctc gagccaggcg aggtcccggc cgtcgccgag
     1681 gagggcgagg tcgcgctcga gctcgaggcg ctgctggtcg gctcgggccg ccaactcgtc
     1741 gatgcggcgg gcacgctcgc gctcggggtc gatctggcgg gcgaggcgct cgatgcggtc
     1801 gatggcgtcg aggtcgagca ccggcgagtc gagcccggcc aggtgggctt cggcctcggt
     1861 gcgctcgtcg acggctcggg tagcggcctc ggcggccgag cggtggtggc cgatggacgt
     1921 gtgcaggcgg gccgacgcat cggccggcaa cgacggcacg gtgccgatct ggtccagctc
     1981 gtggcgggca tcggccgcag cgctccaccg tggccacaac tcgatgatgg ccgtgacgag
     2041 gttcagttcg gaccgcaggc ggtcggcctc ggtgccgagg gtcttggctc ggcccgccag
     2101 cagctccagc tggcggaggt gcgcgggcag ggcacgggac tcgcgctggg cttcgtcgag
     2161 ccggagccga gcctcggcca gctgcgagcg gagctccttg atgcggtagc gctcgccgcg
     2221 gccgttgggc ttgagcagcg cgtcgcgctg gtcgccgagc tgggcgagag cggcttgggc
     2281 actgcgcccg gcgccgagga tgccggcgga gaacacgcgg tcgcggacct ggtcttcggt
     2341 cagtgccttg agctcggcca ggttctccag ccgaaccgag aagatgctct cgaacagctc
     2401 ccgggacgag ccgccgagga cctccgcgag atcaccgttg aacggagcgc catcggcgtg
     2461 cgtgacgcga agcgacgacc gtgccttgct gcggtggatc gtgagctgct cgccggcatg
     2521 ggtggtgatg aacaggcggc cgccgaaggt cccgccgcgc aggggcgggt ggtgctcctt
     2581 cttgctggtc accggtggga agccgaagag catgccgacc aggaagtgct ggagggtggt
     2641 cttcccggac tcgttgggcc cggcgacgat cgtgagaccg tcgcccaggc cctcggcgct
     2701 gtggtcggcg tggacgccgt acccgtcgat gtgccagccg tcgatcttca cgaggatgct
     2761 cccgtcacgg catcgagcgc agcgagccgg gccgcctgcc agatggcgtc gtcgtcgccg
     2821 tcgggcagga ggtcgcccag ttctcggagg acgtcggcag gcaggaggtc ggcccaggtg
     2881 gagc
//
LOCUS       DATMFS010000296         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_104726, whole genome shotgun sequence.
ACCESSION   DATMFS010000296 DATMFS010000000
VERSION     DATMFS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104726"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VNQ33_05435"
     CDS             complement(<1..1124)
                     /locus_tag="VNQ33_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HWJ97581.1"
                     /translation="MGRKSKVRIVGITAAVIAGLAAVPPSARAEPGRPTPTVRTGRSS
                     SAPELIEVGGPATSMTRASTTAGPARHVDVDTSLLKPGVARTVSVDVPDRDLEVALDP
                     IDSGAAGWSAWKGAVAGDPEAVATVVRNGDDVAGIVSTADGSYRIRTTAPGEQVVQEI
                     DRTFPEKADDHVTPPATADLGIAPDDVAAAGSAAETSGDVPAYPVIDVLVAYTPAALT
                     DAGTVSAMNSEIALAIATTNTAYSDSGVNGRVRLAGTTALSENYNLSNLSLNQVTGTA
                     DGHSDSIHALRNSTGADLVSVLVHDPSSCGLAWVLNSVTASASSRSPYGFSTVDYSCA
                     VDNYSFPHELGHNMGLNHDRYVEPSPTLYDYAVGYVNVAAG"
     gene            complement(1182..>2120)
                     /locus_tag="VNQ33_05440"
     CDS             complement(1182..>2120)
                     /locus_tag="VNQ33_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWJ97582.1"
                     /translation="RPRTVHAPGARHDPHLRSPASTTAVRTELDVTYLSATIGAVIRG
                     LDVRDLDDETVAAIRRVWVERRVVFFPGQHLSPAEHVAFASRFGEPTEGHPVIPGIEG
                     NPQVFEIDYTTAGELYATYGDIASYERGVAWHTDVTFVKRPPLGSILRAVHVPEYGGD
                     TLWSDQIAAYDSLSPALQEFLSTLTAIHDGKKQFQGILDLVGEGRWEGETFTALEPVE
                     HPVVRTHPESGEKILFVNPGFTSRIKELSLNESDALLAYLYAHSVKPEHTVRYHWGEG
                     DIGFWDNRSTQHAVAGDFGGSHRVIQRVTLRGDEPR"
BASE COUNT          271 a          682 c          832 g          335 t
ORIGIN      
        1 ccggcggcga cgttcacgta gccgacggcg tagtcgtaga gggtgggcga gggctcgacg
       61 tagcggtcgt ggttcagtcc catgttgtgg cccagctcgt gcgggaagct gtagttgtcg
      121 acggcacacg agtagtcgac cgtcgagaac ccgtacgggc tgcggctcga tgcgctcgcc
      181 gtgacgctgt tgagcaccca ggccaggccg cagctggatg ggtcgtggac gaggaccgag
      241 acgaggtcgg cgccggtgct gttgcgcagg gcgtggatcg agtcggagtg cccgtcggcg
      301 gtgccggtga cctggttcag gctcaggttc gacaggttgt agttctcgct gagggcggtg
      361 gtgccggcca ggcgcacccg gccgttgacg ccgctgtcgc tgtaggcggt gttggtggtg
      421 gcgatggcga gggcgatctc gctgttcatg gcgctgacgg tgccggcgtc ggtcagcgcc
      481 gcgggcgtgt aggccacgag cacgtcgatc actgggtagg ccggcacgtc gcccgacgtc
      541 tcggccgccg agcccgcggc ggcgacgtcg tcgggggcga tgccgagatc ggccgtcgcg
      601 ggcggggtga cgtggtcgtc ggccttctcc gggaacgtgc ggtcgatctc ctgcaccacc
      661 tgctcgcccg gagcggtggt gcggatgcgg tagctgccgt cggccgtgga gacgatgccg
      721 gccacgtcgt cgccgttgcg gacgacggtg gcgacagcct ccgggtcacc ggccacggcg
      781 cccttccagg ccgaccagcc ggcggcgccg ctgtcgatcg ggtcgagggc gacctcgagg
      841 tcgcggtcgg gcacgtcgac cgacacggtg cgggcgacgc ccggcttcag gagcgacgtg
      901 tcgacgtcga cgtggcgcgc cggtcctgcg gtggtcgacg ccctggtcat gctcgtggcc
      961 gggccgccga cctcgatcag ctccggcgcc gacgacgatc ggccggtgcg gaccgtcggg
     1021 gtcggccgac ccggttccgc ccgggcgctc ggcggcaccg cggccagacc ggcgatcacc
     1081 gccgcggtga tgccgacgat cctgaccttg gacttccgac ccatgcctcg tcctcccgcg
     1141 gtgacgcagc ggccgatgct acctcgggcc ggcgtccagg gtcagcgagg ctcgtcgccg
     1201 cgcagggtca cccgttggat cacccggtgc gagccgccga agtcgccggc caccgcgtgc
     1261 tgggtcgagc ggttgtccca gaagccgatg tcgccctcac cccagtggta gcggacggtg
     1321 tgctcgggct tcaccgagtg ggcgtagagg taggcgagga gcgcgtcgct ctcgttgagg
     1381 ctcagctcct tgatccggga cgtgaagccg gggttgacga agaggatctt ctcgccggac
     1441 tccgggtggg tgcgcacgac cgggtgctcg acgggttcca gcgcggtgaa cgtctcgccc
     1501 tcccagcggc cctcgcccac caggtccaag atcccctgga actgcttctt gccgtcgtgg
     1561 atcgcggtga gggtggagag gaactcctgg agcgccgggc tgagcgagtc gtacgccgcg
     1621 atctggtcgg accagagggt gtcaccgccg tactcgggca cgtgcaccgc ccgcaggatc
     1681 gagccgagcg gcgggcgctt cacgaacgtg acgtcggtgt gccaggccac accgcgctcg
     1741 taggaggcga tgtccccgta ggtggcgtag agctcgccgg ccgtcgtgta gtcgatctcg
     1801 aagacctgcg ggttgccctc gatgccgggg atcaccgggt ggccctcggt cggctcgccg
     1861 aaccggctgg cgaacgccac gtgctcggcg ggactcaggt gctggccggg gaagaagacg
     1921 acgcgccgct ccacccacac ccggcggatg gcggcgacgg tctcgtcgtc gaggtcgcgc
     1981 acgtcgaggc cgcggatgac ggcgccgatc gtggccgaca ggtacgtgac gtcgagctcg
     2041 gtgcggacgg cggtggtgga cgcgggggat cggagatgag ggtcatggcg ggctcctgga
     2101 gcgtggacgg ttcgcggtcg
//
LOCUS       DATMFS010000297         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_56462, whole genome shotgun sequence.
ACCESSION   DATMFS010000297 DATMFS010000000
VERSION     DATMFS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56462"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VNQ33_05445"
     CDS             <1..587
                     /locus_tag="VNQ33_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWJ97583.1"
                     /translation="MAIGQKLTATPLQTANAYAALANGGTLYHPSVLEKVTAAQDANA
                     VHRTYKPSVIRTIDWGPNRDAFIAGFKGVVDPNSPYAGGTAVPTFQGFPFESMPLAGK
                     TGTAQTGKYKNGQDRPDNSVFVGFTLGGPTSWVATATLEFSGSGAGAAAPAVRMVLEP
                     IADGSIYQFKVPVGGEIDAEAAAARSSITAGGTD"
     gene            587..1768
                     /gene="rodA"
                     /locus_tag="VNQ33_05450"
     CDS             587..1768
                     /gene="rodA"
                     /locus_tag="VNQ33_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWJ97584.1"
                     /translation="MAATYGTRSPHRVRRPMANFRTNTASPFRHVDLVLVAAVLAVSL
                     FGAVMVYSTTRGAVPPYDAGYLKRVLVFIVLGSGIMAGVALFDYRKLKDFWPFIYGLS
                     LFLLVMVLVPGIGSRTKGTQGWFQLPGFQLQPSELAKLAMIIGFAGLASQFRGDLDNT
                     RVLMLLGLCGAPMFFVLLQPDLGTTLVSVAVSFALLAVAGVRGRILAGLALAAILGTT
                     LVLTSGVLKDYQKARLTTFVEQDKKAATAIDEDTRFNLEQSKIAIGAGGMWGQGLFEG
                     QQTRGKIVPEQRTDFIFSAVGEQLGFVGSGILLALMGTIVWRVWRAAQLARDEFGTLL
                     CTGVMVMLVVQIFENVGMTMGIMPITGIPLPFVSYGGSALLTMFLSMGLVLSVHMRRF
                     S"
     gene            1892..>2159
                     /locus_tag="VNQ33_05455"
     CDS             1892..>2159
                     /locus_tag="VNQ33_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWJ97585.1"
                     /translation="MTVWTDLEPLLAKVQKPARYIGCEDGVYTPDHHAGAASWLLAYP
                     DTYEIGLPNQGLQILAEIINERPDGAAERTYAPWADLEALMRATE"
BASE COUNT          315 a          778 c          711 g          355 t
ORIGIN      
        1 acatggccat cggccagaag ctcaccgcca cgccgctgca gacggccaac gcgtacgccg
       61 ccctcgccaa cggcgggacc ctctaccacc cgagcgtgct cgagaaggtc accgccgccc
      121 aggatgcgaa cgccgtgcac cgcacgtaca agccgtcggt catccgcacc atcgactggg
      181 gtccgaaccg cgatgcgttc atcgccggct tcaagggcgt ggtcgatccc aactccccgt
      241 acgccggcgg gaccgcagtg ccgacgttcc aggggttccc gttcgagagc atgccgctcg
      301 ccggtaagac cgggaccgcc cagaccggca agtacaagaa cgggcaggac cggcccgaca
      361 actcggtgtt cgtcgggttc acgctcggcg gtcccaccag ctgggtggcg acggccacgc
      421 tcgagttctc tggttccggc gctggtgctg ccgcccccgc cgtgcggatg gtcctcgaac
      481 cgatcgccga cggctcgatc taccagttca aggtcccggt cggcggcgag atcgacgccg
      541 aggcggccgc ggcgcgctcc agcatcaccg cagggggcac ggactgatgg ccgccaccta
      601 cggcacccgc agcccgcacc gcgtgcggcg gccgatggcg aacttccgca ccaacacggc
      661 ctcgcccttc cgccacgtcg acctcgtgct ggtcgcggcg gtcctggcgg tgagcctgtt
      721 cggcgcggtg atggtctaca gcaccacccg cggcgcggtt ccgccctacg acgccggcta
      781 cctcaagcgc gtcttggtgt tcatcgtcct cgggtcgggg atcatggccg gcgtcgccct
      841 gttcgactac cgcaagctga aggacttctg gccgttcatc tacggcctga gcctgttcct
      901 gctggtcatg gtgctcgtgc ccggcatcgg ctcgcgcacc aagggcaccc agggctggtt
      961 ccagctgccc ggcttccagc tgcagccttc ggagctggcc aagctggcga tgatcatcgg
     1021 cttcgccggg ctggcctcgc agttccgcgg cgacctcgac aacacccggg tgctgatgct
     1081 gctcggcctg tgcggtgccc cgatgttctt cgtgctgctg cagcccgacc tcgggaccac
     1141 gctcgtgtcg gtcgccgtct cgttcgccct gctcgccgtc gccggggtgc ggggccggat
     1201 cctcgccggg ctcgccctcg cggccatctt gggcaccacc ctcgtgttga cctccggcgt
     1261 cttgaaggac taccagaagg cccggctcac gaccttcgtc gaacaggaca agaaggcggc
     1321 gacggcgatc gacgaagaca cgcggttcaa cctcgaacag tcgaagatcg ccatcggggc
     1381 tggcggcatg tggggccagg gcctcttcga aggccagcag acccgcggca agatcgtccc
     1441 cgagcagcgc accgacttca tcttctcggc ggtgggggag cagctcgggt tcgtgggatc
     1501 ggggatcctg ctcgccctga tgggcacgat cgtgtggcgc gtgtggaggg ccgcccagct
     1561 cgcccgcgac gagttcggca ccctgctgtg cacgggggtc atggtgatgc tcgtcgtcca
     1621 gatcttcgag aacgtcggca tgaccatggg catcatgccc atcaccggca tccccctccc
     1681 gttcgtcagc tacggcggct ccgccctcct cacgatgttc ttgagcatgg gtctggtgct
     1741 gagcgtccac atgcggcggt tcagctagcg ctgtctcgtc actcgctcgc tgcgctcgct
     1801 ccgctcggaa cccagccccg cgcgctccgt gccggtatcg acgggggggg atgacgggtc
     1861 ggtgggtgct cacgggtaga atccggcggt catgactgtc tggaccgatc tcgagccgct
     1921 gctcgccaag gtgcagaagc cagcccgcta catcgggtgc gaggacgggg tgtacacccc
     1981 tgaccaccac gccggcgccg cgagctggct cctggcctac cccgatacct acgagatcgg
     2041 gctgcccaac cagggcctgc agatcctcgc cgagatcatc aacgagcggc ctgacggcgc
     2101 cgccgagcgc acctacgccc cgtgggcgga cctcgaggcg ctgatgcggg cgacggagc
//
LOCUS       DATMFS010000298         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_219791, whole genome shotgun sequence.
ACCESSION   DATMFS010000298 DATMFS010000000
VERSION     DATMFS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219791"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VNQ33_05460"
     CDS             <1..1174
                     /locus_tag="VNQ33_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ97586.1"
                     /translation="ACPDAVNEDNGCIYLGAISDLTKGPFAPLAVPITEAQKAFWDKV
                     NQDGGVGGFDVNIDKYVADAEYNPEIHNKKYQEMRNDILALGQTLGSSQTLAILEDMK
                     ADNVIGVPASWNSAWDFEDQILQSGANYCFEAMNGVDWAVANRGVKGKVLAIHYPNDY
                     GGDAAAGVEAAAKANNLDFKAIETATGQDNQAGAVAAVLKEKPSMLYITTGPAEMATI
                     LGGAAAQGWKGTVVGSSPTWNPALLQTEAAPALEAMYFQAGPWGPWGTESDGHAAMRD
                     ALADVDSPSDGYTAGWVWEYPLLAALKAAADMDGGITRENVVKAATELDSVDYEGMLP
                     DEAFNQTGEPNDVAWRQSVISKVDKEAPTGVSIEQEMQAGPTAEGYDYTEPCFALG"
     gene            complement(1287..>2195)
                     /locus_tag="VNQ33_05465"
     CDS             complement(1287..>2195)
                     /locus_tag="VNQ33_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ97587.1"
                     /translation="FSDYASAVEAEQEAALRAVSAAKADVRRQRQDLVDQQTRQARRD
                     RQGRANAGSLPKIVAGAYQRKAEVSAGRLRGMHEDRLESSRSALAAAEDRVRDDRRIR
                     VDLPDTEVPPRREVLRAEGLVPAFGTTRPLDLDLRGPERIALVGRNGAGKSSLLRAML
                     GLQEPRAGQVTVHVPWRYLPQGVDLLDPELTVLENVRRAAPEAEPHAVRAQLARFLFR
                     GNAVDQVTGTLSGGERWRATLASLLLATPAPQLLVLDEPTNNLDLASVAHLTEVLEAY
                     RGALLVVSHDEPFLDDLALDRRLEVG"
BASE COUNT          342 a          785 c          785 g          283 t
ORIGIN      
        1 ggcgtgtccc gacgccgtga acgaggacaa cggctgcatc tacctgggtg cgatctccga
       61 cctcaccaag ggcccgttcg ctccgctcgc cgtgcccatc accgaggcgc agaaggcgtt
      121 ctgggacaag gtcaatcagg acggcggcgt cggtggcttc gacgtcaaca tcgacaagta
      181 cgtcgcggac gccgagtaca accccgagat ccacaacaag aagtaccagg agatgcgcaa
      241 cgacatcctg gcgctcggcc agaccctggg ctcctcccag accttggcga tcctcgagga
      301 catgaaggca gacaacgtca tcggcgtccc ggcctcgtgg aactcggcat gggacttcga
      361 ggaccagatc ctccagtccg gtgcgaacta ctgcttcgag gccatgaacg gcgtcgactg
      421 ggccgtggcc aaccgcggcg tcaagggcaa ggtcctggcg atccactacc cgaacgacta
      481 cggcggagac gcggccgcgg gcgtcgaggc ggccgcgaag gccaacaacc tcgacttcaa
      541 ggccatcgag accgccacgg gccaggacaa ccaggccggt gccgtcgcgg cggtgctgaa
      601 ggagaagccg tcgatgctct acatcaccac gggtccggcc gagatggcca cgatcctcgg
      661 tggcgcggcc gctcagggct ggaagggcac cgtcgtgggc tcgagcccca cgtggaaccc
      721 ggcgctgctg cagaccgagg ccgctccggc actcgaggcg atgtacttcc aggcgggccc
      781 gtggggcccg tggggcaccg agtcggacgg tcacgcggcc atgcgtgacg cgctcgccga
      841 cgtcgactcg cccagcgacg gctacacggc cggctgggtg tgggagtacc cgctgctcgc
      901 ggcgctgaag gccgcggccg acatggacgg cggcatcacc cgcgagaacg tcgtcaaggc
      961 ggccaccgag ctcgacagcg tcgactacga gggcatgctc cccgacgagg cgttcaacca
     1021 gaccggcgag ccgaacgacg tcgcctggcg tcagagcgtg atctcgaagg tcgacaagga
     1081 ggcgcccacc ggcgtctcga tcgagcagga gatgcaggcc ggcccgacgg ccgagggcta
     1141 cgactacacc gagccctgct tcgccctggg ctgatcagca ctacgtgaag ggggccgacc
     1201 cggctgggtc ggcccccttt cgcgtgcccg cgtacccggc gatcaggtgg cggcgagcgc
     1261 agcgagacgg cgtatcgaga tcccggctac cccacctcga gcctgcggtc gagggcgagg
     1321 tcgtcgagga acggctcgtc gtggctgacc acgagcaggg cgccacggta ggcctcgagc
     1381 acctcggtga ggtgggcgac gctcgcgagg tcgaggttgt tggtcggctc gtcgagcacc
     1441 agcagctggg gcgcgggggt ggccagcagg aggctcgcca gggtggcacg ccagcgctcg
     1501 ccgcccgaca gcgtgccggt cacctggtcg acggcgttgc cccggaacag gaagcgcgcc
     1561 agctgcgcgc gcacggcgtg cggctcggcc tccggcgcgg cccggcgaac gttctccagc
     1621 accgtcagct cgggatcgag caggtccacc ccctgcggca ggtagcgcca cggcacgtgc
     1681 accgtgacct gaccggcccg cggctcctgc agccccagca tcgcgcgcag cagcgacgac
     1741 ttcccggcgc cgttgcggcc cacgagggcg atccgctccg gcccgcgcag atcgaggtcc
     1801 aggggccgtg tggtgccgaa cgccggcacc agcccctcgg cccgcagcac ctcgcgacgc
     1861 ggcggcacct cggtgtccgg caggtccacg cggatccgcc ggtcgtcgcg cacgcggtcc
     1921 tccgcggcgg ccagggccga gcgtgagctc tcgaggcggt cctcgtgcat gccgcgcagt
     1981 cgcccggcgc tgacctcggc cttgcgctgg taggcgccgg ccacgatctt cggcaggctg
     2041 ccggcgttgg cccggccctg gcggtcgcgg cgtgcctgcc gcgtctgctg gtcgacgagg
     2101 tcctggcgct gccggcgcac gtccgccttc gccgcggaca cggcgcgcag cgcggcctcc
     2161 tgctcggcct cgaccgcgga cgcgtagtcg ctgaa
//
LOCUS       DATMFS010000299         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_150696, whole genome shotgun sequence.
ACCESSION   DATMFS010000299 DATMFS010000000
VERSION     DATMFS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150696"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1401
                     /locus_tag="VNQ33_05470"
     CDS             31..1401
                     /locus_tag="VNQ33_05470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ97588.1"
                     /translation="MTETERTDAVVVGAGTAGLQAALQLARTGRSVVVLERRAEGKSG
                     ARWCNGTSAWQFERAGLEPPKPPELRAHGGAAHMVSPTGAHRFTIEDNPVGDADMRLL
                     VDRLQHDAALAGVDIRWGVTDVELRLVHGRPTSVRAVQGTGATAISARLFVDAAGYRG
                     ALRNQVPTLAARCPDVGPSDLCSAQQLILAVDDEAGACRFLADHGARPGDAVVAVGVE
                     GGYSTINITVASSLDEVSVLTGTIPALGHATGPDLVKATRARHPWMGRTVFGGGGTIP
                     LRRVYDRFTAPGIALVGDAACQVFPGHGSGIGFGLLAGKVLAEATAGADDPGGEDVLW
                     RYQAAYLREFGATLAGYDAIRRMSTRLGPEGVEALYATGVFSAPLVRPGLEQKVGLLG
                     PRDTVAAARALAARPALARIVIPALAAMGTAKAVYRTYPATPSAKAFDAWRRAAERLL
                     PRQEAG"
     gene            1449..2483
                     /locus_tag="VNQ33_05475"
     CDS             1449..2483
                     /locus_tag="VNQ33_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97589.1"
                     /translation="MTDRRVEAEVDGIPVWVACDDVGLTDVPEAWGSAFALPSARAGY
                     GLDFDVPVDPSWAVTAQQNIQTTASWWGGTPQPTWTSPSHRLPRWLHRRRPLAPGTGL
                     CFTGGVDSFFSLLRHEQRPTCLVYVVGFDVPVEDRERAERVIALVRDVARDQRTPALI
                     VRTDLREHPRFASISWEHTHGAALAAIGHLLAGTISTLVIPPSYGVDRLVPWGSHPEL
                     DASWSVPGRLDVVHGDASGHRLDRVMAIAHDPLVHRHLRVCWQNVGSDLNCGSCEKCV
                     RTMAMLAAAGTLDSCATFPGRAELPRRIDAMASLPPGTVPMWQDLLEVGLRPEEQAAI
                     EALVARSSAR"
     gene            complement(2472..>3101)
                     /locus_tag="VNQ33_05480"
     CDS             complement(2472..>3101)
                     /locus_tag="VNQ33_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97590.1"
                     /translation="PVTGETLVDIPSGGGYLGAHLPDGVDLVGVEVSDEFVERGREHE
                     VTMFATGLEAAGLPAASADAVVSIAGMHHEDDHGSLFAAWRRLLRPGGRLVAADVVAG
                     SAEARFLDGFVGEWTSTGHAGHYFGDGLAQLGEAAGYVDIAVHDGRYHWWADDDAMLA
                     AFCTSLFGLEDVTADLVLDAIEAGPGLTREPDRVGLRWGLRALVARSAG"
BASE COUNT          399 a         1217 c         1103 g          382 t
ORIGIN      
        1 cgagatcgcc gagcggggcg aggagccggc atgacggaga ccgagcggac cgacgccgtg
       61 gtcgtgggag cgggcacagc cggcctccaa gcggcgctcc agctggccag gaccgggcgc
      121 tccgtcgtgg tgctcgaacg ccgagccgaa ggcaagagcg gcgcccgctg gtgcaacggg
      181 accagcgcct ggcagttcga gcgggccggc ctcgagccgc ccaagccacc cgagctgcgg
      241 gcgcacggcg gggccgccca catggtgagc cccaccggcg cccaccgctt caccatcgag
      301 gacaacccgg tcggcgacgc cgacatgcgg ctgctggtcg atcggctgca gcacgacgcc
      361 gcgctggccg gggtcgacat ccgctggggc gtcaccgacg tcgagctgcg gctggtccac
      421 ggccgaccga cctcggtgcg cgccgtgcag ggcacgggcg ccaccgccat cagcgcccgc
      481 ctgttcgtcg atgccgccgg ttaccgaggc gccttgcgga accaggtccc gacgctggcc
      541 gcccgctgcc ccgacgtcgg gcccagcgac ctgtgctcgg cccagcagct catcttggcg
      601 gtcgacgacg aggcgggcgc ctgccggttc ctggccgacc acggtgcccg acccggagac
      661 gcggtggtcg ccgtcggggt cgagggcggc tactccacca tcaacatcac ggtggcgtcg
      721 tcgctcgacg aggtgtcggt gctgaccggg acgatcccgg cgctgggcca cgccaccggc
      781 ccggacctgg tgaaagccac ccgggcccgc cacccctgga tggggcgcac cgtgttcggc
      841 ggcggcggca cgatcccgct gcggcgcgtc tacgaccggt tcaccgcgcc gggcatcgcc
      901 ctcgtcggcg acgccgcctg ccaggtgttc ccgggccacg gcagcggcat cggcttcggc
      961 ctcctggccg ggaaggtcct ggccgaagcc accgctggcg ccgacgaccc cggcggcgag
     1021 gacgtgctgt ggcggtacca ggccgcgtac ctccgggagt tcggcgccac gctggccggt
     1081 tacgacgcca tccgccgcat gagcacgcgg ctggggcccg agggggtcga ggccctctac
     1141 gccaccggcg tgttctccgc gccgctcgtg cgtccgggcc tcgagcagaa ggtcggcctg
     1201 ctgggcccgc gggacacggt cgccgccgct cgggccctcg ccgcccggcc tgccctggcc
     1261 cgcatcgtga tccccgccct ggccgccatg ggcacggcga aagccgtcta ccgcacctac
     1321 ccggccacgc cgtcggcgaa ggcgttcgac gcgtggcgcc gggccgccga gcgcctgctc
     1381 ccccgtcagg aagccgggtg agccgaccgc ccacggcggg accggcgtgg gccgtccgga
     1441 gccgccaggt gaccgatcgc cgggtcgagg ccgaggtcga cggcatcccc gtctgggtgg
     1501 cgtgcgacga cgtcgggctg accgacgtcc ccgaagcatg gggctcggcg ttcgcgctcc
     1561 cgtcggcgcg agccgggtac ggcctggact tcgacgttcc cgtggatccc agctgggcgg
     1621 tcacggcgca gcagaacatc cagaccaccg ccagctggtg gggcggcacg ccgcagccga
     1681 cgtggaccag cccgagccac cgcctcccga ggtggctgca ccgccgccgg cccctcgccc
     1741 ccggcaccgg gctgtgcttc accggcggcg tcgattcgtt cttctcgctg ctgcggcacg
     1801 agcagcggcc cacctgcctc gtctacgtcg tcggcttcga cgtcccggtc gaagaccgcg
     1861 agcgggcgga gcgcgtcatc gccctcgtcc gagacgtggc gagggaccag cggacacccg
     1921 cgctcatcgt gcggaccgac cttcgcgagc acccgcgctt cgcctcgatc agctgggagc
     1981 acacccacgg tgccgccctc gccgccatcg gccacctcct ggccggcacc atctccacgc
     2041 tcgtgatccc gccgtcctac ggcgtcgacc ggctggtccc gtggggctcg caccccgagc
     2101 tcgacgcctc ctggagcgtc cccggccgcc tcgacgtcgt gcacggcgac gcgtccggcc
     2161 accggctgga ccgggtgatg gcgatcgccc acgacccgct cgtgcaccgg cacctccggg
     2221 tctgctggca gaacgtcggg tccgacctga actgcggctc gtgcgagaag tgcgtgcgga
     2281 cgatggcgat gctcgccgcc gccggcacgc tcgactcgtg cgccaccttc ccgggccgcg
     2341 ccgagctgcc gcgccgcatc gacgccatgg cctcgctgcc tccggggacc gtgccgatgt
     2401 ggcaggacct gctcgaggtc gggctgcggc ccgaggagca ggccgcgatc gaggcgctcg
     2461 tcgcccgcag ctcagcccgc tgagcgggcg acgagcgcgc gcaggcccca gcgcagaccc
     2521 acccggtccg gctcacgggt caggcccggg ccggcctcga tcgcgtcgag gaccagatcg
     2581 gccgtcacgt cctccagccc gaacaggctc gtgcagaacg cggcgagcat cgcatcgtcg
     2641 tcggcccacc agtggtagcg gccgtcgtgc acggcgatgt cgacgtaccc ggcggcttcg
     2701 cccagctgcg ccaagccatc cccgaagtag tgcccggcat gtccggtcga ggtccactcg
     2761 cccacgaagc cgtcgaggaa ccgggcctcg gccgaaccgg ccaccacgtc cgcggcgacg
     2821 agacggcctc cagggcgcag cagccgccgc caggcggcga agagcgagcc atggtcgtcc
     2881 tcgtggtgca tgccggcgat cgacaccacg gcatcggcgc tcgcggccgg gaggccggcc
     2941 gcctccagcc ccgtggcgaa catcgtgacc tcgtgctccc gcccccgctc gacgaactcg
     3001 tccgagacct cgaccccgac caggtccacg ccgtcgggca ggtgggcgcc gaggtatccg
     3061 ccgcccgacg ggatgtccac cagcgtctcg ccggtcactg g
//
LOCUS       DATMFS010000300         3954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_34296, whole genome shotgun sequence.
ACCESSION   DATMFS010000300 DATMFS010000000
VERSION     DATMFS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34296"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VNQ33_05485"
     CDS             <1..586
                     /locus_tag="VNQ33_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97591.1"
                     /translation="YPTFGPAAEPTEAQRRVRTTALVKTLSARLVEVKGAYGLYLGRA
                     PDPLGQDYWVRGLRNGTQTVDTLAANLISSREFLAKRTDGTPGSWVRAVYSLVFGRAP
                     DPGGLTFWTGRASALGPKRTAARLIASPESRRYQIRSISSSVAVLGGPPEVHIVTDGV
                     ADWAAGGYDRMLADLLATDDVYLHAQRIHRVHPA"
     gene            complement(735..1544)
                     /locus_tag="VNQ33_05490"
     CDS             complement(735..1544)
                     /locus_tag="VNQ33_05490"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWJ97592.1"
                     /translation="MLLAIDIGNTQTVIGLFGDDDDADGGPVGDSPAEAGLLDHWRIA
                     TNAERTSDEHALVVQEFLGFHGFSFDDDIDGIAISSVVPRTTAAFREMTEKYFGFRPV
                     VIEPGVKTGLPILTENPREVGADRIANAVAALDLYGGPAIIIDFGTATTYDALSAKGE
                     YLGGAIAPGIEISLDALYTRAAALRRVELIEPRSVIGRTTVESIQSGAVFGFAGQVDG
                     ICRRLADELGDPAVVATGGLAELIAPYTSSIKQVEPWLTLHGLRLVFERNQ"
     gene            complement(1547..1816)
                     /locus_tag="VNQ33_05495"
     CDS             complement(1547..1816)
                     /locus_tag="VNQ33_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97593.1"
                     /translation="MTTEPAEPTAAASEPVASPAAVPERSRRRPAPVARWIRGLPRAT
                     YAALLLAVIVVLLLAAVLVNPGERVGPVRAADPPVGGPVLPATRL"
     gene            complement(1813..3261)
                     /locus_tag="VNQ33_05500"
     CDS             complement(1813..3261)
                     /locus_tag="VNQ33_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ97594.1"
                     /translation="MSRPSAAAGEGPPRHGLAALAGVGLLGVFAAFWRLDRADWKLDE
                     DAYAQAGWMLVHDGVDPNLGHPPLAKLLFGGAQVLLGRNLFAVRTVSALGFLAAVAVL
                     FVFGRRVAGWWTGIAAAGLFAVVPRSMVVGGWNVAELRIDRYGLLEAVAGTLVLVGLW
                     LGWRWITSGGWRWATGAGAAIGLAGASKLNALAVLVPVVVVGLAYVWGRARLASEAGM
                     LVGAAVGAFLLPFAVFGSRAFDQIDQTLRFPADRAKGGHLLVLGSDVYVRSPWWAHLK
                     YQLDADGPILAAALLVGLACVLLSRRRLVVVYLLGATLSLVVTAMASPVALPHYRAIW
                     TAPLVLLVAIGLTEQVERLARHRRLEPVPVVAAVALVVLGAFGLGSMVQLATLGDGDY
                     RQMAVLARADGVEPRRIVIYSEAVAPYFPGSVDALAPFDDGSTPAQMVVLDPSLSDAV
                     TPDVVARWRGWARGWGLVPHRVGRLEAWWAEP"
     gene            complement(3278..>3954)
                     /gene="nadC"
                     /locus_tag="VNQ33_05505"
     CDS             complement(3278..>3954)
                     /gene="nadC"
                     /locus_tag="VNQ33_05505"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWJ97595.1"
                     /translation="QIDDEVHVVWQVGDGDLVDARQTIGVVTGPLASILTAERTALNF
                     LGHLSGIATRTRAFVDAAAPLRVWDTRKTTPGLRALEKAAVRAGGARNHRGNLSDWVM
                     FKDNHLTALGIVEAVRSARDRWPGRTVHVECERIEQVVEALDAGADALLLDNMAPDEV
                     RAAVVVAGEHAAANGTRRALLEVSGGITLENVGSYSATGADTVSSGSLTNSSPVLDIG
                     LDIEVR"
BASE COUNT          595 a         1455 c         1439 g          465 t
ORIGIN      
        1 ctaccccacc ttcgggcccg cggccgagcc caccgaggcc cagcggaggg tccgcaccac
       61 ggcgctggtc aagaccctga gcgcccgtct cgtcgaggtg aagggggcgt acggcctcta
      121 cctgggccgg gcgccggatc ccctgggcca ggactactgg gtccgcggcc tccgcaacgg
      181 cacccagacg gtcgacacct tggccgccaa cctgatcagc tcccgcgagt tcctcgcgaa
      241 gcgcaccgac ggcacccccg gcagctgggt gcgagcggtc tactcgctcg tgttcggccg
      301 agcccccgat cccggcgggc tgacgttctg gaccggtcga gcctcggccc tgggaccgaa
      361 gcgcaccgcc gcccgcctca tcgcctcgcc cgagtcacgc cggtaccaga tccgctcgat
      421 ctcgagctcg gtcgccgtgc tcggcgggcc gcccgaggtc cacatcgtga ccgacggcgt
      481 cgccgactgg gctgccggcg gctacgaccg catgctcgcc gacctcctcg ccaccgacga
      541 cgtctacctc cacgcccagc gcatccatcg agtgcacccg gcctgaggcc tccgaccagc
      601 gccgaccacg agcgcgaggt gatgggtacc gagcgaagcg agccctcgtc tgggggtagg
      661 ccgtcaggcc ttcatcgagt tcgaggaacg gagagaggcg cagccgaacg agcgacgaga
      721 gggcggagcc cgatctactg gttgcgttcg aacaccaggc ggaggccgtg cagcgtcagc
      781 cagggctcga cctgcttgat ggacgacgtg tacggagcga tgagctcggc gaggccgccg
      841 gtggcgacca cggcgggatc gcccagctcg tcggcgaggc ggcggcagat gccgtcgacc
      901 tgtccggcga acccgaacac cgcacccgac tggatcgact cgacggtggt gcggccgatg
      961 acgctgcgcg gttcgatcag ctcgacgcgg cgcagggcgg cggcccgggt gtagagggcg
     1021 tcgaggctga tctcgatgcc gggcgcgatg gcgccgccca ggtactcgcc cttggccgag
     1081 agggcgtcgt aggtggtggc ggtgccgaag tcgatgatga tcgccggacc gccgtagagg
     1141 tccagcgcgg cgacggcgtt ggcgatgcgg tcggcgccca cctcgcgcgg gttctcggtg
     1201 aggatcggca gacccgtctt caccccgggc tcgatgacga cgggccggaa gccgaagtac
     1261 ttctcggtca tctcccggaa cgccgcggtg gtgcggggga cgaccgacga gatggcgatg
     1321 ccgtcgatgt cgtcgtcgaa ggagaagccg tggaagccga ggaactcctg gacgacgagg
     1381 gcgtgctcgt cggaggtgcg ctcggcgttg gtggcgatac gccagtggtc gagcaggccg
     1441 gcttcggcgg ggctgtcgcc gacgggcccg ccgtcggcat cgtcgtcgtc gccgaacagg
     1501 ccgatcacgg tctgggtgtt gccgatgtcg atcgccagga gcatcgctag agcctcgtag
     1561 ctgggaggac ggggccgcca actggtggat cggcggcccg gaccgggccg acccgctcgc
     1621 cggggttgac caagacggcg gcgagcagca gcacgacgat gaccgccagc agcagcgcgg
     1681 cgtacgtcgc ccgcggtagt ccgcggatcc agcgggcgac gggagcgggt cgccgccgtg
     1741 agcgctcggg caccgctgcc ggggacgcca cgggctcgga ggccgccgcg gtgggctcgg
     1801 ccggctcggt ggtcacggct cggcccacca ggcctcgagg cgcccgacac ggtgcgggac
     1861 caggccccac ccccgggccc agccccgcca gcgggccacc acgtcgggcg tcaccgcatc
     1921 gctcagggac gggtcgagga cgaccatctg ggcgggcgtg ctgccgtcat cgaatggagc
     1981 cagggcatcg acggatccgg ggaagtaggg agcgacggcc tcgctgtaga tcacgatgcg
     2041 ccggggctcg acgccgtcgg ccctcgccag caccgccatc tggcggtagt cgccgtcgcc
     2101 cagggtcgcc agctgcacca tcgagccgag gccgaacgcg ccgagcacga ccagggccac
     2161 cgcggcgacc accggcaccg gctcgagccg gcggtgccgg gcgagccgct ccacctgctc
     2221 ggtgaggccg atggccacca gcaacacgag cggcgccgtc cagatcgccc ggtagtgagg
     2281 cagggccacc ggcgatgcca tggcggtgac gaccaggctg agggtcgcgc ccagcaggta
     2341 gacgacgacc aggcgccgcc ggctgagcag cacgcacgcc aggcccacca gcaaggcggc
     2401 ggccaggatc ggcccgtcgg cgtcgagctg gtacttcagg tgcgcccacc acgggctgcg
     2461 cacgtagaca tcggagccca gcaccagcag gtgcccgccc ttggcccggt cggccgggaa
     2521 ccgcagggtc tggtcgatct ggtcgaacgc ccggctgccg aacacggcga acggcaggag
     2581 gaaggcgccg acggccgcgc cgaccagcat ccccgcctcg ctcgccagcc tcgcccggcc
     2641 ccacacgtag gccagcccga cgaccaccac cgggacgagc acggcgaggg cgttgagctt
     2701 cgaggcgccg gccaggccga tggcggctcc cgcaccggtg gcccaccgcc agccacccga
     2761 cgtgatccac cgccagccga gccacagacc gaccagcacg agcgtccccg ccacggcctc
     2821 cagcaggccg tagcggtcga tccgcagctc ggccacgttc cacccgccca ccaccatcga
     2881 ccgggggacg acggcgaaca ggccggcggc ggcgatcccc gtccaccagc cggcgacccg
     2941 gcgcccgaac acgaacagca cggccacggc ggccaggaac ccgagcgccg agacggtccg
     3001 cacggcgaac aggttccggc cgagcagcac ctgggccccg ccgaacagca gcttggccaa
     3061 cggcgggtgg cccaggttcg ggtcgacccc gtcgtgcacg agcatccagc ccgcctgggc
     3121 gtaggcgtcc tcgtcgagct tccagtcggc ccggtcgagc cgccagaagg cagcgaagac
     3181 gccgagcagg ccgacaccgg ccagcgccgc cagcccgtgc cgcggcggcc cctcgccggc
     3241 ggccgcgctc ggacggctca cgcccggcgg ctcggcatca gcgcacctcg atgtcgaggc
     3301 cgatgtcgag caccggcgac gagttggtga gcgagccgct ggacacggtg tcggcgccgg
     3361 tcgccgagta cgagccgacg ttctccaggg tgatgccgcc cgacacctcg agcagcgccc
     3421 gtcgggtgcc gttggcagcg gcgtgctccc cggccaccac gacagcggcg cggacctcgt
     3481 cgggcgccat gttgtccagc agcagggcgt cggccccggc gtcgagcgcc tcgacgacct
     3541 gctcgatgcg ttcgcactcc acgtgcaccg tccgccccgg ccaccggtcg cgggcagacc
     3601 ggaccgcttc cacgatgccg agggcggtca ggtggttgtc cttgaacatc acccagtcgc
     3661 tgaggttgcc gcggtggttg cgcgcgccac ccgcacgaac ggcggccttc tccagcgccc
     3721 gcagccccgg cgtggtcttg cgggtgtccc agacccggag cggcgcggcg gcgtcgacga
     3781 aggcccgggt gcgggtggcg atgccgctca ggtgacccag gaagttcagc gcggtgcgct
     3841 cggcggtgag gatcgaggcc agcggacccg tcacgacgcc gatggtctgg cgggcatcga
     3901 cgaggtcgcc gtcgcccacc tgccacacca cgtggacctc gtcgtcgatc tggc
//
LOCUS       DATMFS010000301         2276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80468, whole genome shotgun sequence.
ACCESSION   DATMFS010000301 DATMFS010000000
VERSION     DATMFS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80468"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="larC"
                     /locus_tag="VNQ33_05510"
     CDS             complement(<1..1149)
                     /gene="larC"
                     /locus_tag="VNQ33_05510"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWJ97596.1"
                     /translation="MTTAWFHCFSGIAGDMALGSLIDAGADLDEVKELLGRLPISDWK
                     LEAEPVLRGGIAGTKVHVHATDSTVVRTASHIQGLVAEARLPERVQQRAAAVFEALAQ
                     AEGRLHRRPPDQVHFHEVGGIDAIVDVVGTCAALEVLEVDTIACSPVAVGLGMVRAAH
                     GIIPNPAPAVVELLAGIPTRGVDLSVELTTPTGAALASALASAYGPMPAMTITATGFG
                     AGTKELDHRPNLTQVVLGVVADAPAADGQPVMLLEANVDDVTGEVLGHAIGAVLAAGA
                     HDAWVTPIVMKKGRPAHTIHALVDPALLPQVMGVLAAETGTLGVRGHRLERWPLPRRH
                     ERVVVDDLSVRVKVSPGRAKAEFDDAAQVAERTGRPVRDVIAEAERRWS"
     gene            complement(1146..1943)
                     /gene="larB"
                     /locus_tag="VNQ33_05515"
     CDS             complement(1146..1943)
                     /gene="larB"
                     /locus_tag="VNQ33_05515"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWJ97597.1"
                     /translation="MDEAAIRRLLEQVRAGETSPDDAVDALRRLPYADLGFATVDHHR
                     QVRQGMAEAIYGPGKTPDQVARIAAELLARSDEAAVVLTRADLAQVQAAVEANPGGDV
                     CGSTVVWRAVAERPERVVLLTAGTADLPVADECAATLTAHGLRPVRITDVGVAGIHRL
                     LARTDELIDADAVIVVAGMEGALASVVGGLTGAPVIAVPTSVGYGAGLDGVTALLGML
                     ASCANGITVVGIDNGYGAACAVARLFVSPQAQRRVAAPSSTSSRAAL"
     gene            1963..>2276
                     /locus_tag="VNQ33_05520"
     CDS             1963..>2276
                     /locus_tag="VNQ33_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HWJ97598.1"
                     /translation="MNDLAQPKPPSDAARPPRVGLLGPRGTFAEQALLTQPDLADHER
                     VRYATMADVLRATEDGEVELGFVAIENSIEGTVNVTSDTLAFDVDLLIQREIVIPIQQ
                     HL"
BASE COUNT          297 a          817 c          871 g          291 t
ORIGIN      
        1 gctccaccgg cgctcggcct cggcgatcac gtcgcgcaca ggacgcccgg tccgctcggc
       61 cacctgggcg gcgtcgtcga actcggcctt ggcccgaccc gggctgacct tcacccgcac
      121 cgacaggtcg tcgaccacca cccgctcgtg gcgccgcggg agcggccagc gctcgagccg
      181 gtggccgcgg accccgaggg tgccggtctc ggcggcgagc acgcccatca cctgggggag
      241 gagggcgggg tcgaccaggg cgtggatcgt gtgcgccggc cggcccttct tcatcacgat
      301 gggggtcacc caggcgtcgt gcgcaccggc ggcgagcacg gcgccgatgg cgtggcccag
      361 cacctcgccg gtcacgtcgt cgacgttggc ctcgagcagc atcacgggct ggccgtcggc
      421 ggcgggcgcg tcggcgacca cgcccaagac gacctgggtg aggttgggcc ggtggtccag
      481 ctccttggtg ccggcgccga acccggtggc cgtgatggtc atcgcgggca tcggcccgta
      541 cgcgctggcg agggccgagg cgagggccgc tccggtgggg gtggtgagct ccacggacaa
      601 gtcgacgccc cgggtcggga tgccggcgag cagctcgacc accgcgggcg ccgggttcgg
      661 gatgatcccg tgggcggcgc ggaccatgcc gaggccgacg gcgacggggg agcaggcgat
      721 ggtgtccacc tcgagcacct cgagggcggc gcacgtgccg acgacgtcga cgatggcgtc
      781 gatgccgccc acctcgtgga agtggacctg gtccggtgga cggcggtgga gccggccttc
      841 ggcctgggcg agggcctcga acacggcggc ggcccgctgc tgcacccgct ccgggaggcg
      901 ggcctcggcc accaggccct ggatgtgcga tgccgtgcgc acgacggtgg agtcggtggc
      961 gtgcacgtgg accttggtgc cggcgatgcc gccccgcagc acgggctcgg cctcgagctt
     1021 ccagtcgctg atcggcaggc ggccgagcag ctccttcacc tcgtcgaggt cggccccggc
     1081 atcgatcagc gagccgagcg ccatgtcccc ggcgatgccc gagaagcagt ggaaccaggc
     1141 cgtggtcaca gcgctgcccg cgacgaggtc gatgacggag cggcgacccg gcgctgggcc
     1201 tggggcgaga cgaacaggcg ggcgacggcg caggcggcgc cgtacccgtt gtcgatgccg
     1261 accacggtga tgccgttggc gcacgaagcc agcatcccga gcagcgcggt gacgccgtcg
     1321 agcccggcgc cgtacccgac gctggtgggc acggcgatga ccggcgcccc ggtcaggccg
     1381 cccacgacgc tggcgagggc gccttccatg ccggcgacca cgatgacggc atcggcgtcg
     1441 atcagctcgt cggtccgggc caggagccgg tggatgccgg ccacgccgac gtcggtgatg
     1501 cggaccggac gcaggccgtg ggcggtgagg gtggcggcgc actcgtcggc cacgggcagg
     1561 tcagccgtgc cggcggtgag cagcaccacg cgctcgggtc gctcggccac cgctcgccac
     1621 accaccgtgg agccgcacac gtcgccgccc ggattggcct cgacggcggc ctggacctgg
     1681 gcgaggtcgg cccgggtgag cacgacggcg gcctcgtcgg agcgggccag gagctcggcg
     1741 gcgatgcggg ccacctggtc cggtgtcttg cccgggccgt agatcgcctc ggccatgccc
     1801 tggcgcacct ggcggtggtg gtcgacggtc gcgaacccga ggtcggcgta cggcaggcgg
     1861 cggagcgcgt cgacggcatc gtccggggag gtctcgcccg cacggacctg ctcgagcaat
     1921 cgtcggattg cggcctcgtc cacgcctcta tcctggcccg tcgtgaacga cctggcgcag
     1981 ccgaagcccc cgtccgacgc tgcccgcccg ccgcgggtcg gcctgttggg gccccgcggc
     2041 acgtttgcgg agcaggccct gctgacccag ccggacctcg ccgatcacga gcgggtccgc
     2101 tacgccacca tggctgacgt cctccgggcc accgaggacg gcgaggtcga gctgggcttc
     2161 gtcgccatcg agaactccat cgagggcacg gtcaacgtca cctcggacac gctcgccttc
     2221 gacgtcgacc tgctgatcca gcgggagatc gtcatcccga tccagcagca cctcat
//
LOCUS       DATMFS010000302         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_56521, whole genome shotgun sequence.
ACCESSION   DATMFS010000302 DATMFS010000000
VERSION     DATMFS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56521"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VNQ33_05525"
     CDS             complement(<1..652)
                     /locus_tag="VNQ33_05525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWJ97599.1"
                     /translation="MSVDAVEVHGTVAPGFERVAEAFASNFVEHGEVGAACAVRVGGE
                     EVVDIWAGIADPATGRPYEGDTLQLVFSSTKGIVAAAAHVLIDRGLLDPEAPVARYWP
                     EFAANGKADIPVRWVLGHRSGVIGIDRVLSVEELSSWDPFVEALAAAAPLWEPNTAHG
                     YHALSFGFLVGEIVRRITGTDIGTFVRNEVAGPVGAEFLIGVTDDELPRVAPLIGAV"
     gene            734..2029
                     /locus_tag="VNQ33_05530"
     CDS             734..2029
                     /locus_tag="VNQ33_05530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ97600.1"
                     /translation="MRATIPPDDAVLWQEQVEVPRLPRRTGTTDDRVEVAVVGGGYAG
                     ISAARVLAAAGRGAVVVEKGPLGWGAHSRNGGMAIPELKAGPAALATRYGPLGLRMHQ
                     EVNEAFDHLEALIAGEGIACDYERTGQLYLAHSPRLVPSLRSLAAEHADQFGEPVRFV
                     PREALAEEIGSTAWHGGVVFDRTGGLHPAKLHAGLVRLALDAGAEVRDRTAATRLTDR
                     GAGHRGGRFLLDTEHGTIRADDVIVTTNAYADDLLPELARRVLPIGSFIVATEPLDPA
                     LADEISPRRRMFVDTKHLLFYWRLSPDGRMVFGGRRSLQHATVAEAADHLTASMREIH
                     PQLRDVAVTHRWGGSVAVTLDRMPHVGQVRGAWYATGCNGSGVALNTWLGHRLGQVVL
                     GQAPPPSFAELKHRPIPLHRFRKLYLPLVGQWFRYQDRP"
     gene            complement(2030..>2662)
                     /locus_tag="VNQ33_05535"
     CDS             complement(2030..>2662)
                     /locus_tag="VNQ33_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ97601.1"
                     /translation="VVATLTEAGYYNSGQDCTAPCRVIAGPGLYDDLVGSLASAAGAL
                     ATGDPFDEATAVGPVVSAEQRDRVAGMVERASADGAEIVTGGRALDGDGFFYAPTVVA
                     GADQRSEIVQREVFGPVISVQRFADEQQALAWANDVDYGLAASVWTRDVGRAMRMARD
                     LRFGTVWVNDHIPIVSEMPHGGFKHSGYGKDMSIHAIEHYTELKHVMIKL"
BASE COUNT          367 a         1023 c          917 g          355 t
ORIGIN      
        1 tgacggcgcc gatcaacggg gcgacgcggg gcagctcgtc gtcggtcacc ccgatgagga
       61 actcggcgcc gaccggcccg gcgacctcgt tgcgcacgaa cgtcccgatg tcggtccccg
      121 tgatgcgccg gacgatctcg ccgacgagga agccgaacga cagcgcgtgg tagccgtgcg
      181 ccgtgttcgg ctcccacagg ggcgcagctg cggcgagcgc ctcgacgaac gggtcccagg
      241 aggagagctc ctccacggac agcacccggt caatgccgat gacgccggaa cggtggccga
      301 ggacccagcg caccgggatg tcggccttgc cgttggcggc gaactccggc cagtaccgcg
      361 cgaccggagc ctcgggatcg agcaggccgc ggtcgatcag cacgtgggcc gccgcggcca
      421 cgatgccctt cgtcgacgag aagacgagct gcagggtgtc cccctcgtag ggacggccgg
      481 tggcggggtc ggcgatgccg gcccagatgt cgaccacctc ctcgccgccg acgcggaccg
      541 cgcaggccgc accgacctcg ccgtgctcga cgaagttgct ggcgaacgcc tccgccaccc
      601 gctcgaaccc cggtgcgacc gtgccgtgga cctcgaccgc atcgaccgac atgtgacctc
      661 ccgaggtggc tcgaacgctg cgagcgtatt cgccccctcc atcgcggccg tcacgcgccc
      721 gtacgatccg ggtgtgcgtg ccacgatccc accggacgac gccgtgctgt ggcaggagca
      781 ggtcgaggtc ccgcgcctgc cacggcgtac cggcacgacg gacgaccgcg tcgaggtggc
      841 cgtcgtgggt ggcgggtacg ccgggatctc ggcggcgcgg gtccttgccg ccgccggccg
      901 cggcgcggtg gtcgtcgaga agggcccgct gggctggggc gcccacagcc gcaacggcgg
      961 catggccatc cccgagctga aggccggtcc cgccgccctg gccacgaggt acgggcccct
     1021 cggcctgcgc atgcaccagg aggtcaacga ggcgttcgac cacctggagg cgctgatcgc
     1081 gggcgagggc atcgcctgcg actacgagcg gaccgggcag ctctacctcg cccactcccc
     1141 ccgcctggtc ccctccctgc ggtcgctggc ggccgagcac gccgaccagt tcggcgagcc
     1201 agtgcggttc gtgccccgcg aggcgctcgc cgaggagatc ggttcgaccg cctggcacgg
     1261 cggcgtcgtg ttcgaccgca ccggcggcct ccaccccgcc aagctccacg ccgggctggt
     1321 ccgcctcgcc ctcgacgccg gcgccgaggt ccgagatcgc accgccgcca cgcgactcac
     1381 cgatcgcggg gccggccacc gcggcggacg gttcctgctg gacaccgagc acggcaccat
     1441 ccgagccgat gacgtgatcg tcacgaccaa cgcctacgcc gacgacctgc tccctgagct
     1501 ggcccggcgg gtcctgccga tcggcagctt catcgtcgcc accgagccgc tggaccccgc
     1561 gctggccgat gagatcagcc cccggcgccg gatgttcgtc gacaccaagc acctgctctt
     1621 ctactggcgc ctctcccccg acggccgcat ggtcttcggc ggccgccgca gcctccagca
     1681 cgccaccgtc gccgaggccg ccgaccacct gaccgcatcg atgcgggaga tccaccccca
     1741 gctccgcgac gtcgccgtca cccaccggtg gggcggcagc gtcgccgtca ccctcgaccg
     1801 gatgccccac gtcggccagg tccgcggcgc ctggtacgcc accggctgca acggctcggg
     1861 cgtggcgctc aacacctggc tcggccaccg cctcggccag gtcgtcctcg gccaggcccc
     1921 gcccccgtcg ttcgccgagc tgaagcaccg cccgatcccg ctccaccggt tccggaagct
     1981 ctacctcccg ctcgtcggcc agtggttccg ctaccaggac cggccgtagt cagagcttga
     2041 tcatcacgtg cttgagctcg gtgtagtgct cgatggcgtg gatcgacatg tccttgccgt
     2101 agccggagtg cttgaagccg ccgtggggca tctccgagac gatcgggatg tggtcgttga
     2161 cccagacggt gccgaacctc aggtcccgtg ccatgcgcat ggcccggccg acgtcgcggg
     2221 tccagaccga ggcggccagg ccgtagtcga cgtcgttggc ccaagcgagg gcctgctgct
     2281 cgtcggcgaa gcgctggacc gagatgaccg gtccgaacac ctcgcgctgg acgatctcgc
     2341 tgcgctggtc ggcacccgcc acgaccgtgg gggcgtagaa gaacccgtcg ccgtcgaggg
     2401 cccggccccc ggtcacgatc tcggcaccgt cggcgctggc ccgctcgacc atccccgcca
     2461 cccggtctcg ctgctcggcc gacacgacgg gtcccacggc cgtcgcctcg tcgaacgggt
     2521 cgcccgtcgc cagcgccccg gcggctgagg cgagcgagcc gacgaggtcg tcgtagaggc
     2581 cggggccggc gatgacccgg cacggcgcgg tgcagtcctg gcccgagttg tagtagccgg
     2641 cttcggtgag cgtggccacg ac
//
LOCUS       DATMFS010000303         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_242052, whole genome shotgun sequence.
ACCESSION   DATMFS010000303 DATMFS010000000
VERSION     DATMFS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242052"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VNQ33_05540"
     CDS             <1..633
                     /locus_tag="VNQ33_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ97602.1"
                     /translation="LIGPNGAGKTTTFNVITGLQPPTRGHVLLDGNDITGVSAHQRAR
                     GGIARTFQRLELFGSLTVRDNVLTAAELRRETGTDPQAATDQLLARVGLTAVAGQRAD
                     SLSTGSARLVELARALACHPRVLLLDEPASGLDEHETDRFAEILVDLTGDGLGILLVE
                     HDVPLVMRLCAQITVLNFGEVLAAGTPIEVQKNPDVIDAYLGQDPGAPVG"
     gene            630..1346
                     /locus_tag="VNQ33_05545"
     CDS             630..1346
                     /locus_tag="VNQ33_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97603.1"
                     /translation="MTELTAPLLELRNVHAAYGRIQVLHGIDLVVPRGQVVALLGPNG
                     AGKTTTLLVATGQHPASKGCVHVGGRHVNGVKPDELARLGLCTIPEGRGVFRSLTVRE
                     NLVMASYRGTPAEQIIDDAVARFPRLGERIGQVAGTLSGGEQQMLAMARGLATDPGLL
                     IIDELSMGLAPLIVAELYEIVAGIATEGVSILVVEQFARTVLGVADHAVIMQHGHVVA
                     AGPPADLEAELSAAYLGAST"
     gene            1343..1732
                     /locus_tag="VNQ33_05550"
     CDS             1343..1732
                     /locus_tag="VNQ33_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97604.1"
                     /translation="MTDLTASNEPSAADRLELGVEDLHVPEPSADAEGLLLKIGLVLP
                     VIGVILILVAYWNASGSEYVADQVPMLISGGILGIGLAIIGVGLFIRFSLARLLRFWL
                     ARMVVEQQAQTDRVVQALGRIEAKLEK"
     gene            complement(1733..2299)
                     /locus_tag="VNQ33_05555"
     CDS             complement(1733..2299)
                     /locus_tag="VNQ33_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97605.1"
                     /translation="MSRLPDLDPLQFTEAQGRLASDLANRPEVQANGIVGPFAAWMHA
                     PALGEPLAALGTGIRFGASLPANATEVAICTTATFYGSSFEASAHQPLALRAGVDGAA
                     LGRLFAGDEPGFEGDDAAAHAVASELLRTHRISDAAHADAVARFGSQGVVELVTTVGY
                     YALNALLLNGFEIPLAPGMDDPLAGSPG"
     gene            complement(2579..>2903)
                     /locus_tag="VNQ33_05560"
     CDS             complement(2579..>2903)
                     /locus_tag="VNQ33_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97606.1"
                     /translation="VFRMGMFHPNHPDGNYETANLVEAFDPPTAISWRTGTEDADTGE
                     LSFGGWFWRYDLAPVGPDRTEVTLTYDWSAVGPEVHEYLSFPPFGPDHLANSLEHLAA
                     VVAAR"
BASE COUNT          406 a         1106 c          991 g          400 t
ORIGIN      
        1 ctgatcgggc ccaacggcgc cggcaagacc accacgttca acgtcatcac cgggctccag
       61 ccgccgacgc gcggccacgt gctgctcgac gggaacgaca tcaccggcgt gtccgcgcac
      121 cagcgggccc gcggcggcat cgcccgcacg ttccagcggc tggagctgtt cgggtcgctg
      181 accgttcgcg acaacgtcct caccgccgcc gagctccggc gcgagacggg caccgatccg
      241 caggccgcca ccgatcagct gctcgcccgg gtgggtctca ccgcggtcgc cggtcagcgc
      301 gccgattcgc tctccacggg ctcggcccgc ctggtggagc tggcccgggc cctggcgtgc
      361 cacccccgcg tgctgctgct cgacgagccg gcgtcgggcc tcgacgagca cgagaccgac
      421 cgcttcgccg agatcctcgt cgacctcacc ggcgacggcc tcggcatctt gctcgtcgag
      481 cacgacgtcc ccctcgtcat gcgcctgtgc gcccagatca ccgtgttgaa cttcggcgag
      541 gtcctggcgg cgggaacgcc gatcgaggtc cagaagaacc ccgacgtgat cgatgcctac
      601 ctcggccagg acccaggagc acccgtcgga tgaccgagct caccgccccc ctcctggagc
      661 tgcgcaacgt ccacgccgcc tacgggcgca tccaggtgct ccacggcatc gacctcgtgg
      721 tgccccgcgg ccaggtggtg gcgctgctcg ggcccaacgg cgccggcaag accaccacct
      781 tgctcgtggc caccggccag cacccggcgt cgaagggctg cgtccacgtc ggcggccgcc
      841 acgtcaacgg cgtgaagccc gacgagctcg cccgcctcgg cctgtgcacc atccccgagg
      901 gccgcggcgt cttccgcagc ctgaccgtgc gcgagaacct ggtgatggcc agctaccgcg
      961 gcacgccggc cgagcagatc atcgacgacg ccgtcgcccg gttcccccgc ctcggcgagc
     1021 gcatcggcca ggtggccggc accctctcgg gcggcgagca gcagatgctc gccatggccc
     1081 ggggcctcgc caccgacccg gggctgctca tcatcgacga gctgtccatg ggcctggcgc
     1141 cgctcatcgt cgccgagctc tacgagatcg tcgccgggat cgccaccgaa ggcgtgtcga
     1201 tcctggtcgt cgagcagttc gcccgcaccg tcctcggtgt cgccgaccac gccgtgatca
     1261 tgcagcacgg ccacgtcgtc gccgccggcc cacccgctga cctcgaagcc gagctctcgg
     1321 ctgcctacct cggagcatcc acatgaccga cctgaccgcc tccaacgagc ccagcgccgc
     1381 cgaccgcctc gagctcggcg tcgaagacct ccacgtcccc gagccgtcgg ccgacgccga
     1441 gggcctgctc ctgaagatcg ggctggtcct gccggtcatc ggcgtgatcc tgatcctcgt
     1501 cgcctactgg aacgcctcgg gctccgagta cgtggccgac caggtgccga tgctcatctc
     1561 gggcggcatc ttgggcatcg gcctggccat catcggcgtc ggcctcttca tccggttctc
     1621 gctggcccgg ctcctgcgct tctggctggc ccgcatggtg gtcgagcagc aggcccagac
     1681 cgaccgtgtg gtccaggccc tgggccgcat cgaggccaag ctcgagaagt gatcagccag
     1741 gcgaccctgc cagcgggtcg tccatgccgg gtgcgagcgg gatctcgaac ccgttgagca
     1801 gcagggcgtt gagagcgtag tagcccacgg tcgtcaccag ctcgacgacc ccctgcgaac
     1861 cgaaccgcgc caccgcgtcc gcatgggcgg catcgctgat gcggtgtgtc cgcaggagct
     1921 cgctggccac ggcgtgagcg gcggcgtcgt cgccctcgaa ccccggctcg tccccggcga
     1981 agaggcgccc gagcgccgcc ccgtcgaccc cggcccggag ggcgagcggc tggtgggccg
     2041 acgcctcgaa gctcgacccg tagaacgttg cggtcgtgca gatcgccacc tcggtggcgt
     2101 tcgcgggcag cgaggcgccg aaacggatcc cggtcccgag cgcggccagc ggctcgccca
     2161 gcgccggtgc gtgcatccag gcggcgaacg ggccgacgat gccgttggcc tgcacctccg
     2221 gccggttcgc caggtcgctc gcgagccgcc cttgcgcctc ggtgaactgc agcggatcga
     2281 gatcgggaag ccggctcatc tcgccaccgt cgcacaccgg gccgccccac gcccgaacgc
     2341 tcccgcgtcc acgcacggag gcgatggcgg acggcgcgcc ggtgtccccc gccgagagag
     2401 ccgggctcct cgatctgctg ccgtcggggc ggtggatggg catgcggcca cgatcggccc
     2461 ccacatgatc gtgaccaccc tcgaacgggc gccgtccaag ccccgaacgg cgggcccggc
     2521 ggacggtcgc tggtgccccg tcccagctga gcggtgaccg ctgccgcgag gcgacgagct
     2581 acctggcggc gaccacggcg gccaggtgct cgagcgagtt ggcgaggtgg tccggaccga
     2641 acggcggaaa ggagaggtac tcgtgcacct cggggccgac cgccgaccag tcgtaggtca
     2701 aggtgacctc ggtgcggtca gggccgaccg gtgcgaggtc gtagcgccag aaccagccgc
     2761 cgaagctgag ctcgccggtg tcggcgtcct cggtgccggt ccgccaggag atggcggtgg
     2821 gcgggtcgaa cgcctcgacg aggttggccg tctcgtagtt cccgtcgggg tggttcgggt
     2881 ggaacatccc catccggaac acc
//
LOCUS       DATMFS010000304         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_220007, whole genome shotgun sequence.
ACCESSION   DATMFS010000304 DATMFS010000000
VERSION     DATMFS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220007"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VNQ33_05565"
     CDS             complement(<1..343)
                     /locus_tag="VNQ33_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97607.1"
                     /translation="MENLFPVLPFRSALYTPAELFAGFSQEDPASYATTPDLLAYRST
                     LLPPNREVGMLRALHDQSITEQRTVLLEGRKVVAVMGGHALHRDAPAYREVAVLARTL
                     TRAGFLVVSGGG"
     gene            544..1311
                     /locus_tag="VNQ33_05570"
     CDS             544..1311
                     /locus_tag="VNQ33_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWJ97608.1"
                     /translation="MTDDELAAGVEHFRETGWLLTQTLDEAGVAELQEWVDEVASWPD
                     DGEGWMHHRELTDDGPKLCRSENIIPFHPGLRDLLTTGALLGTASALLGQQAVLYKEK
                     VNYKLAGGAGYAPHQDAPAYPFIASHVSCMVAVDDATLENGCLEVVEGMYHDVLAMDD
                     AGCIDPDLVASYEWKAVEVPAGWTLWFHSRTPHRSGPNDSPVPRRALYPTYNALAEGD
                     LRADYYAEKLARFTAEGGRADGKVQVSLINDFQGRAV"
     gene            1311..1862
                     /locus_tag="VNQ33_05575"
     CDS             1311..1862
                     /locus_tag="VNQ33_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWJ97609.1"
                     /translation="MPAGGVEEVLALFEAWGTRNYDEDVSQLDHALQTAALARSEGAD
                     DALVAAALLHDVGHLLELRDGGVDDGQTEVDLGHEGRGARWLAPVFPSQVTGPIALHV
                     AAKRYRCAIDPAYHDGLSDGSRRSLVRQGGPMSPDEAARFAAHPACADAVALRGWDDG
                     GKVEDLDVPGLASYRGLLEQLAR"
     gene            2016..2366
                     /locus_tag="VNQ33_05580"
     CDS             2016..2366
                     /locus_tag="VNQ33_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97610.1"
                     /translation="MEHGASIFLRWAGRILILVAVFIVVASLFLPKAVSPLGSAVCPS
                     GTTLDNTRYTPPAAPKNERLELVCTSRTYTETAAPKILLVSGGLGVAGIVAIFFSGRI
                     VRPQTKGPVVPGTF"
BASE COUNT          372 a          930 c          864 g          325 t
ORIGIN      
        1 gtccgccgcc cgacaccacg aggaacccgg cccgggtgag cgtcctggcg agcaccgcca
       61 cctcgcggta cgccggagcg tcgcggtgga gcgcgtggcc gcccatgacg gccaccacct
      121 tgcggccctc cagcaggacg gtccgctgct cggtgatgga ctggtcgtgc agcgcccgca
      181 gcatgccaac ctcccggttg ggcggcagca gcgtcgagcg gtaggcgagc aggtccggcg
      241 tggtggcgta ggacgccgga tcctcctgcg agaacccggc gaacagctcc gccggcgtgt
      301 acagcgccga ccggaacggc agcacgggga agaggttctc caccgtcatc tcgtcactcg
      361 ctcgctgcgc tcacgctgct ccgcctctcc gggccttcaa ggcccgatcg gtcatgacca
      421 cccgaggcac acagggatcg gacgccctcg atgacaccat cccccgccct cgctgtccaa
      481 cgagcgaagg gttcaacggg cttcgaggcg ggggcagcgg gcagaattgc ccgcatgcac
      541 cccatgaccg atgacgaact ggctgccggt gtcgagcact ttcgggagac ggggtggctg
      601 ttgacgcaga cgctcgacga ggccggcgtc gccgagctgc aggagtgggt cgacgaggtg
      661 gcgtcgtggc ccgacgacgg cgaggggtgg atgcaccacc gcgagctcac cgacgacggg
      721 ccgaagctgt gccggagcga gaacatcatc ccgttccacc ccggcctccg ggacctgctc
      781 accaccggcg cgctgctcgg caccgcgtcg gcgctgctcg gccagcaggc ggtgctctac
      841 aaggagaagg tcaactacaa gctggccggc ggcgccggct acgccccgca ccaggacgcc
      901 ccggcctacc cgttcatcgc ctcccacgtc tcgtgcatgg tggcggtcga cgacgccacg
      961 ctggagaacg gctgcctcga ggtggtcgag ggcatgtacc acgacgtcct cgccatggac
     1021 gacgccgggt gcatcgaccc cgacctggtc gccagctacg agtggaaggc ggtcgaggtg
     1081 cccgcggggt ggacgctctg gttccactcg cggacaccgc accgcagcgg cccgaacgac
     1141 tcccccgtgc cgcgccgggc gctgtacccc acctacaacg cgctggccga aggcgacctg
     1201 cgggccgact actacgccga gaagctggcc cggttcacgg ccgagggcgg ccgggccgac
     1261 ggcaaggtgc aggtgtcgct catcaacgac ttccagggcc gagcggtctg atgccggcgg
     1321 gtggcgtcga ggaggtgctc gccttgttcg aggcctgggg cacccggaac tacgacgagg
     1381 acgtgtcgca gctcgaccac gcgctgcaga ccgccgccct cgcccggtcc gaaggggccg
     1441 acgacgcact cgtcgccgcc gcgctgctgc acgacgtcgg ccacctcctc gaactgcgcg
     1501 acggcggcgt cgacgacggg cagaccgagg tcgacctcgg ccacgagggg cgcggggccc
     1561 ggtggctcgc cccggtgttc ccgtcgcagg tcaccgggcc gatcgccctc cacgtggccg
     1621 ccaagcgcta ccgctgcgcc atcgacccgg cctaccacga cgggctgtcc gacggatccc
     1681 gccggagcct ggtccgccag ggcgggccga tgtcgcccga tgaggcggca cgcttcgccg
     1741 cccacccggc ctgcgcggac gccgtggcgc tgcgcggctg ggacgacggc ggcaaggtcg
     1801 aggacctcga cgtgcccggc ctggcctcct accggggcct gctggagcag ctggcccgct
     1861 gaccgccgcg gcaggcaagg acgccgcggg tcccgccctc ctcggaccgc acgagcgcgt
     1921 ggcccgagcc accacacgtt gcgtgaaccc gatgtcacgg aaaatggtcc cgacgtccct
     1981 gtcaggacgt ccgaaccttc tctacagtgg ccgcaatgga acacggtgca agcatcttcc
     2041 tccgctgggc aggccggatc ctgatcctcg tcgcggtgtt catcgtcgtc gccagcctct
     2101 tcctgcccaa ggccgtgtcg ccgctcggct cggccgtgtg cccgagcggg accacgctcg
     2161 acaacacccg ctacacccca cccgccgcac cgaagaacga gcggctcgag ctggtctgca
     2221 cgagccggac ctacacggag accgccgcac ccaagatcct cctcgtctct ggcggcctgg
     2281 gggttgcggg catcgtcgcc atcttcttca gcgggcgcat cgtgcggccg cagacgaagg
     2341 gccccgtcgt ccccggcacg ttctgaccgg gctccgaccg gtctccggcg gcacccggcg
     2401 gcatccgacg gcgccgccga gcgtcagcgc cgcggtgcga aggcaggcag caccggacgg
     2461 ccgaccggga ggtcatcggg cgcgaccggg t
//
LOCUS       DATMFS010000305         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_56632, whole genome shotgun sequence.
ACCESSION   DATMFS010000305 DATMFS010000000
VERSION     DATMFS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56632"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VNQ33_05585"
     CDS             complement(<1..240)
                     /locus_tag="VNQ33_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HWJ97611.1"
                     /translation="MTTTQLTIHVPGNHLMRPLLGERDELLRMVEAAFSGAGVHVRGN
                     EIVIGGDDADRVGRLFGELVTLLQAGQEIDPALLAR"
     gene            371..940
                     /locus_tag="VNQ33_05590"
     CDS             371..940
                     /locus_tag="VNQ33_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97612.1"
                     /translation="MDETTDDSADGPTDQALRDLLAAEQHRQRVEERRRRHGQRQRAD
                     ETVSLADILGALAAHGALVSLELASGPVPPSTICEVGRDYVAVRADGEAVRLLPMAAI
                     AAASPVADAPATPPRSGAPVHRGPPLHLAERLRELAARHARVQLAAGPRSLTGRLRRV
                     GLDVAVLELDDGTDVFVSLAPLPEAVVHP"
     gene            complement(947..1195)
                     /locus_tag="VNQ33_05595"
     CDS             complement(947..1195)
                     /locus_tag="VNQ33_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ97613.1"
                     /translation="MSTPIQWLSTAEAAKYLGITPRTLYRFIDEGQIAGYRFGRVIRL
                     KQDDVDLFIEASRITPGTLEHLYPEVVGSADKSGDVED"
     gene            1429..>2296
                     /locus_tag="VNQ33_05600"
     CDS             1429..>2296
                     /locus_tag="VNQ33_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97614.1"
                     /translation="MPGRDRSSGTDAPVHRAAVVALVEASDGRGVAAALGLARALAAT
                     AASATEVLLADLTLDAPHRAVHGLDPEATGLSELAAASRFGPPPASLVRDAAHVVAGG
                     YQVLPGLRHHRDWVQIGKRSAATAIEAVRHHAAVVVAPIDRDLEGEPDTGSFDIEDRN
                     VLARTVVRTADLVVVAGALDQAGRPGLQATLAALAAFHVPLDRTLVLTSGPSPRRRAH
                     RLTPATRFLHLGAGARAERRLGTAVRAHLAEVGEAGTGAVPDDAPQRIAPGTLGAGPQ
                     DVEGWITPSVPQQ"
BASE COUNT          293 a          847 c          833 g          323 t
ORIGIN      
        1 gcgcgccaga agcgccgggt cgatctcctg accggcctgg agcagggtca ccagctcccc
       61 gaacaagcgg ccgacgcggt ctgcatcatc gccgccgatg acgatctcgt tgccgcgcac
      121 gtggacgccg gcgcccgaga aggcagcctc gaccatgcgg agcagctcgt ctcgctcgcc
      181 gagcaacggc cgcatgaggt ggttgccggg gacgtggatg gtgagttggg tggtcgtcaa
      241 agctggcctt ctgcgctggt gcgccaagcg tactgccggg ggaccaggct cccgatgggt
      301 ttgcgccgtc tgccactgtc acggagcaac cccttcacac ggcgcgggcg agtgcgtacc
      361 ctcccggccc gtggacgaga cgaccgacga cagcgccgac ggccccacgg accaggcgct
      421 ccgcgacctg ctggccgccg agcagcaccg ccagcgcgtc gaggagcggc gccggcgcca
      481 cgggcagcgc cagcgggccg acgagaccgt gtcgctcgcc gacatcctcg gcgccctcgc
      541 cgcccacggc gcgctggtgt cgctcgagct cgcctcgggt cccgtgccgc cgtcgacgat
      601 ctgcgaggtg ggacgggact acgtcgcggt ccgggcggac ggcgaggccg tccgcctgct
      661 gccgatggct gcgatcgccg cagcatcgcc ggtcgcggac gcacccgcga cgccgccccg
      721 gtcgggagca cccgtccacc gcggcccgcc gctgcacctg gcggagcggc tgcgggagct
      781 ggccgcccgc cacgcgcggg tgcagctggc cgcaggccca cggtcgctga ccgggcgact
      841 gcggcgggtc ggactcgacg tcgccgtcct cgagctcgac gacgggacgg acgtgttcgt
      901 gtccctggcg ccgctgcccg aggcggtggt gcacccgtga gccggctcag tcctccacgt
      961 cgccgctctt gtcggcggaa ccgacgacct cggggtagag gtgctcgagg gtgcccggtg
     1021 tgatccggct tgcctcgatg aagaggtcga catcgtcctg cttgaggcgg atcacgcgac
     1081 cgaagcggta gccggcgatc tgcccctcgt cgatgaagcg gtagagcgtg cgcggggtga
     1141 tgccgaggta cttcgccgct tcagcggtgc tcagccattg gattggggtg gacatgcacg
     1201 ggatcgtagc gccctcaggc cctttcatgg cggaccatgc cgatcgtttg gcgccctgtc
     1261 catgtctttc catgttccgg acatgtttca caccacccca gcagcaaggt cagcctgaga
     1321 acccgagaag gagaggctca cacggtgcgg tatcgacccg atgtccccgt ccgcgcatgg
     1381 ctcggccgca cgggccgctc gcgatcggcg gccgccaccg tcggctccgt gcccggccga
     1441 gaccgctcgt ctggcaccga cgctccggtc caccgggcgg cggtcgtcgc gctggtggag
     1501 gcctcggacg gccggggtgt ggcggctgct ctggggctgg cccgggccct tgccgccacg
     1561 gcggcgagcg cgaccgaggt cctgctcgcc gacttgaccc tcgatgctcc gcaccgggcc
     1621 gtccacggcc tcgacccgga ggccaccggc ctctcggagc tcgccgcggc gagtcggttc
     1681 ggccctcccc cggcatcgct ggtccgcgat gccgcccatg tcgtcgcggg cgggtaccag
     1741 gtgctccctg ggctccggca ccaccgcgac tgggtccaga tcggcaagcg gtcggccgcg
     1801 acggccatcg aggcggtccg gcaccatgct gccgtggtcg tcgcgcccat cgaccgggac
     1861 ctcgaaggtg aaccggacac cggatcgttc gacatcgagg accgcaacgt gctcgcccgg
     1921 accgtggttc gaacggcaga cctggtggtg gtcgccggtg cgttggacca ggcaggacgg
     1981 cccggcctgc aagcgacgct ggcggcgctc gccgcgttcc acgtcccgct cgatcggacg
     2041 ctggtcctca cctcgggtcc gtcaccgcgc cgccgagccc accggctgac gcccgcaacc
     2101 cggttcctgc acctcggagc cggggcgcgg gccgaacgtc gcctcgggac cgcggtgcgc
     2161 gcccacctgg ccgaggtcgg cgaggccggt accggtgccg tccctgatga cgcgccgcag
     2221 cggatcgcgc ccgggacgct cggcgccggg cctcaggacg tggagggctg gatcacgccg
     2281 agcgtgccgc agcagc
//
LOCUS       DATMFS010000306         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_127153, whole genome shotgun sequence.
ACCESSION   DATMFS010000306 DATMFS010000000
VERSION     DATMFS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127153"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VNQ33_05605"
     CDS             complement(<1..326)
                     /locus_tag="VNQ33_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97615.1"
                     /translation="MHVATRWLLDRWMMHPRIALALRAAVAAAIAWILVRFVPGSQDY
                     PYYAPFGAIIATTSTLAGSVRESLQAVGAIVVGGVIAWLVDTATGDYVAAVTLALVVA
                     ISVSVAG"
     gene            425..1207
                     /locus_tag="VNQ33_05610"
     CDS             425..1207
                     /locus_tag="VNQ33_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ97616.1"
                     /translation="MSALSLTPENPRGILLFAPGAGGDPTRYRRLLDAAAEAGFIVHA
                     PEHELTETFSEDVVRRRVEALVAPLAAIGRDDLPVVTAGHSLGGCAALCLAGARPRTR
                     SGEPFEVPSEPRVSRVVVLAPATGWFAGPDALDGVTAPVTAFVGSEDTVTPPESAELL
                     RPAPAGLDLRSYDGSATSTSCRRCPRTRCRPPVSTRTRSTSPSSGTSSPPSADPAVRR
                     LFVTRSATRRPPVADASACVHPAPTDWCPRCARGARIERTTR"
     gene            1204..>2292
                     /locus_tag="VNQ33_05615"
     CDS             1204..>2292
                     /locus_tag="VNQ33_05615"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWJ97617.1"
                     /translation="MKNPMGRARSRRMSLAGMAIAVAAAGCLGTVTTGAASAAPAGIV
                     RAADEPTYFAPLVSADTTWRYLATGVDPSPGADRHAWAQPGFDDSAWSTGKGGFGAKW
                     DGGVESPAYDSGMRATTLLPMRPTGGDNIPTYFFRTTFEVTPEQLATGEDIRFDGYVD
                     DAAVVFVNGVEVKRHLVEEATPNVAYDDGTGGDLEFTIPASAFTAGTNTIAVRLHQDR
                     ATSSDIWFSLDHLGGELPPPPPGPAVDAPSRVILTPTDRPETSQSFTWLAGAPEVTNG
                     RVQLRVGDGPIRSVAARSQGTAVNQANPHFSATVGGLEPATEYTYRVGNPDAWSEWKT
                     FSTADPDAKDFEYVYYGDAQIGLDTTWPA"
BASE COUNT          309 a          904 c          781 g          298 t
ORIGIN      
        1 cccgcgacgc tcaccgagat cgccaccacg agggccagcg tgacggccgc gacgtagtcg
       61 cccgtggccg tgtcgacgag ccaggcgatc acgccgccga cgacgatcgc cccgaccgcc
      121 tgcagcgact cgcgcaccga gcccgcgagg gtcgaggtcg tggcgatgat ggcgccgaag
      181 ggcgcgtagt acggatagtc ctgcgatccc gggacgaagc gcacgaggat ccacgcgatc
      241 gcggcagcca cggccgcgcg cagcgcgagg gcgatccggg ggtgcatcat ccaccggtcg
      301 aggagccatc gggtcgctac gtgcatcacc tcaccgtggc acgagccgcc gacggcggca
      361 cgtcgtcccc gctcagccgt tgccgcgcgg gttctgaccg gcgtcttctg cgtagggtgc
      421 cagcatgagc gccctctccc tgacccccga gaacccgcgc ggcatcctgc tcttcgcgcc
      481 cggagccggt ggcgatccca cgcgctaccg ccggctgctc gacgcggccg cggaggcggg
      541 gttcatcgtc cacgccccgg agcacgagct gaccgagacg ttctccgagg acgtcgtgcg
      601 ccggagggtg gaggcactcg tcgcgccgct cgccgcgatc ggccgcgacg acctgcccgt
      661 cgtgacggcg ggtcactcgc tcggcggctg cgcggccctg tgcctcgcgg gggcgcgccc
      721 ccgcacgcgc tccggtgagc cgttcgaggt cccgtccgag ccgcgcgtct cgcgtgtcgt
      781 ggtgctggcg ccggcgaccg ggtggttcgc cggccccgac gccctcgacg gcgtgaccgc
      841 gcccgtcacc gccttcgtcg gatcggagga caccgtcacc ccgcccgagt cggccgagct
      901 gctgcgcccg gcgccggccg ggctcgacct gcgctcctac gacgggtcgg ccacttcgac
      961 ttcatgtcgc cgctgccctc gaacaaggtg ccgacccccg gtctcgaccc ggacgcgttc
     1021 tacgagtcct tcatccggga cttcctcgcc gccatcggct gacccggccg tccgtcgact
     1081 gttcgtgacg aggtcggcga cccgtcggcc gccggtggcc gacgcgtccg cctgcgttca
     1141 cccggcaccg actgactggt gcccgaggtg cgcacgtggc gcacgcatcg agaggacaac
     1201 cagatgaaga accccatggg ccgggcccgc tcccggcgca tgtcgctggc cggcatggcg
     1261 atcgcggtcg ccgcggcggg ctgcctcggg acggtcacca cgggcgccgc ctcggcagcc
     1321 cccgccggca tcgtccgggc ggccgacgag ccgacctact tcgccccgct ggtctccgcc
     1381 gacacgacgt ggcgctacct ggccaccggc gtcgacccct ccccgggcgc cgaccggcac
     1441 gcctgggccc agcccggctt cgacgactcc gcctggtcca ccggcaaggg cggcttcggc
     1501 gccaagtggg acggtggcgt cgagtccccg gcctacgact cgggcatgcg ggcgaccacg
     1561 ctgctgccga tgcggcccac cggcggcgac aacatcccca cctacttctt ccgcaccacc
     1621 ttcgaggtga cccccgagca gctggccacg ggcgaggaca tccgcttcga cggctacgtc
     1681 gacgacgccg cggtggtgtt cgtcaacggc gtggaggtca agcgccacct cgtcgaggag
     1741 gccacgccca acgtcgcgta cgacgacggc accggcggcg acctcgagtt cacgatcccg
     1801 gcctcggcgt tcaccgccgg caccaacacg atcgcggtgc gcctgcacca ggatcgggcc
     1861 accagctcgg acatctggtt ctccctcgac cacctcggcg gcgagctgcc gcccccgccg
     1921 ccgggtccgg cggtggacgc gcccagccgc gtgatcctca ccccgaccga tcgtcccgag
     1981 acctcgcaga gcttcacgtg gctcgcggga gcccccgagg tcacgaacgg tcgcgtccag
     2041 ctgcgtgtcg gcgacggccc gatccggtcc gtggcggcgc ggagccaggg caccgcggtc
     2101 aaccaggcga acccgcactt ctcggcgacg gtcggcggcc tcgagcccgc caccgagtac
     2161 acgtaccgcg tgggcaaccc cgacgcgtgg agcgagtgga agaccttcag cacggccgat
     2221 cccgacgcga aggacttcga gtacgtctac tacggcgacg cccagatcgg cctcgacacc
     2281 acctggccgg cc
//
LOCUS       DATMFS010000307         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_127320, whole genome shotgun sequence.
ACCESSION   DATMFS010000307 DATMFS010000000
VERSION     DATMFS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127320"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..566)
                     /locus_tag="VNQ33_05620"
     CDS             complement(51..566)
                     /locus_tag="VNQ33_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWJ97618.1"
                     /translation="MADPLPETVVVDDVPSDADRAQVAALLGRAPKGAFTVVVRHVHD
                     GSPVVIRNEPLLDDGRPMPTRYWLLGEPERTMVSRIESRGGVHRVEDEVGLDVIAAAH
                     DRYRTERDAALPVGWDGPTPSAGVGGTRVGVKCLHAHYGWWLAGGDDPVGQWVADHLA
                     SGDGDRDRIVP"
     gene            complement(731..2008)
                     /locus_tag="VNQ33_05625"
     CDS             complement(731..2008)
                     /locus_tag="VNQ33_05625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97619.1"
                     /translation="MDFAADPDHEAIREGVRRTCSDFDDGYWRRCDDDHEFPWDFYRA
                     MADGGWIGIAIPEAYGGGGRGITEASIVVEEVAASGAAMNGASAIHLSIFGMGPVIKH
                     GSDELKARVLPAVASGELHVCFGVTEPDAGTDTSRITTRAVLADDGSHYLVRGRKVWT
                     TKAPHCQKVLLLVRTTPRDEAAKPTDGMTLLLADLQIPQTEVGVRNEAEGRFAHQLRH
                     GSGPGSVPVPGRVRPEVEITPIPKAGRNAVVSCEVVYDDLPVPVADRVGDEGDGFRYL
                     LDGLNPERILIAAEALGTGRAALRRAVAYANERVVFDRPIGQNQGIAFPLAQAHMELR
                     AAELAIREAAWRYDSGLPCGEAANTAKYLAAEAGYRAADQAVQTLGGMGYAGEYHVER
                     YWREARLMRLAPVSQELIQAYIATNVLGLPRSY"
     gene            complement(2035..2397)
                     /locus_tag="VNQ33_05630"
     CDS             complement(2035..2397)
                     /locus_tag="VNQ33_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWJ97620.1"
                     /translation="MAPRGKAKARRRAIAGLIVTVLLVAVLLFAVFPTRSWLAQRKET
                     SAAQQELADLKAKRSATDAAKKRLENDEYIEQQARGELGYVHPGEEAYNILPAPTDPI
                     GLPDGWPFTGVERVFGAD"
     gene            complement(2398..>3403)
                     /gene="eno"
                     /locus_tag="VNQ33_05635"
     CDS             complement(2398..>3403)
                     /gene="eno"
                     /locus_tag="VNQ33_05635"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWJ97621.1"
                     /translation="ELDGTDNKARLGANAILGASLAIAKAAAIELDVPLYRHVGGTNA
                     HVLPVPMMNVLNGGAHADNNVDFQEFMVMPVGAASFSEALQWGTETYHVLKMVLHDKG
                     LSTAVGDEGGFAPDLADNEEPLKLLVQAIEQAGFTPGTDIAIALDPASSEVFQDGAYV
                     LEGEGRTLSGAELADYWADLCDRYPIVSIEDGMAEEDWDGWAALTAKIGDRVQLVGDD
                     LFVTNTTRLARGIEAGSANSILIKVNQIGTLTETLEAVSLANRNRYTAVMSHRSGETE
                     DATIADLAVATNCGQIKTGAPARSDRVAKYNQLLRIEDGLGDAAAFLGRSAFNQGAKG
                     "
BASE COUNT          445 a         1196 c         1233 g          529 t
ORIGIN      
        1 cagcaggcgc gtcgagttgg tcccgatgtc gatggcggcc acacgctcgg tcacgggacg
       61 atcctgtcac ggtcgccgtc gccggaggcc aggtggtcgg cgacccactg cccgaccgga
      121 tcgtcgcctc cggcgagcca ccagccgtag tgggcgtgca ggcacttgac gcccacccgc
      181 gtgccaccga cccccgctga cggcgtcggc ccatcccacc ccacgggcaa ggcggcatcg
      241 cgctcggtgc ggtagcggtc gtgggcggcg gcgatcacgt cgaggccgac ctcgtcttcg
      301 acccggtgca ccccgccgcg gctctcgatg cggctgacca tcgtccgctc gggctcgccc
      361 aggagccagt accgggtcgg catgggccgt ccgtcgtcga gcagcggctc gttgcggatc
      421 acgaccggcg agccgtcgtg cacgtggcgc accacgaccg tgaaggcccc cttgggcgcc
      481 cggccgagga gtgcagccac ctgcgcccgg tcggcgtcgc tcgggacatc gtcgaccacg
      541 acggtctcgg ggaggggatc ggccatggat cgaggctacg gcggagcgca gccgcctccc
      601 agacgtggtt cacgtcgggg cgcagcccga ctggtcggcc aagctcacca cggcgaacgg
      661 cgagccggcg atgatcgccg ccctcgtctc gagcaccggc tacgacggcc gttcgtgacg
      721 ctccggccgg tcagtagctc cggggcaggc cgaggacgtt ggtggcgatg taggcctgga
      781 tcagttcctg gctgaccggc gccaggcgca tgaggcgggc ctcgcgccag tagcgctcga
      841 cgtggtactc gccggcgtac cccatgccgc ccagggtctg gaccgcctgg tccgcggccc
      901 ggtacccggc ctcggcggcg aggtacttgg cggtgttggc ggcctcgccg cagggcaggc
      961 cgctgtcgta gcgccacgcc gcctcgcgga tggccagctc cgccgcccgc agctccatgt
     1021 gcgcttgggc cagcgggaag gcgatgccct ggttctggcc gatcggccgg tcgaagacca
     1081 cgcgctcgtt ggcgtaggcc acggcccggc gcagggcggc ccgccccgtg cccagcgcct
     1141 cggccgcgat gaggatgcgc tcggggttga gcccgtccag caggtagcgg aacccgtcgc
     1201 cctcgtcgcc cacgcgatcg gccaccggta cgggcaggtc gtcgtacacg acctcgcacg
     1261 acacgacggc gttgcgcccg gccttcggga tcggggtgat ctccacctcc ggccgcaccc
     1321 gccccggcac cggcaccgac cccggccccg acccatgccg aagttggtgt gcgaaccgac
     1381 cctctgcctc gtttcgcaca ccaacttcgg tctgggggat ctggaggtcc gcgagcagga
     1441 gggtcatgcc gtcggtcggc ttggcggcct cgtcccgtgg cgtcgtgcgc accagcagga
     1501 gcaccttctg gcagtgcggc gccttcgtgg tccagacctt ccgaccgcgg acgaggtagt
     1561 gcgagccgtc gtcggcgagc accgcccgcg tcgtgatgcg cgacgtgtcg gtcccggcgt
     1621 cgggctcggt gaccccgaag cagacgtgca gctcacccga tgccaccgcg ggcagcaccc
     1681 gggccttcag ctcgtctgag ccgtgcttga tgacgggacc catcccgaag atcgacaggt
     1741 ggatggccga ggcgccgttc atcgccgcgc ccgaggccgc cacctcctcg accacgatcg
     1801 atgcttcggt gatcccgcgc ccgccgccgc cgtacgcctc ggggatggcg atgccgatcc
     1861 agccgccgtc ggccatcgcc cggtagaagt cccaggggaa ctcgtggtcg tcgtcgcagc
     1921 ggcgccagta gccgtcgtcg aagtccgagc acgttcgccg cacgccctcg cggatcgcct
     1981 cgtgatcggg atcagccgcg aagtccatcg gcgccgaacc taggcgaggt cgtctcagtc
     2041 agctccgaag acgcgctcga cacccgtgaa gggccagccg tccggaaggc cgatcgggtc
     2101 ggtgggtgcc gggaggatgt tgtacgcctc ttcgcccgga tggacgtagc ccagctcgcc
     2161 gcgggcctgc tgctcgatgt actcgtcgtt ctcgagccgc ttcttggcgg cgtcggtggc
     2221 ggaccgcttc gccttgaggt cggccagctc ctgttgggca gcggacgtct ccttgcgctg
     2281 ggcgagccac gaccgcgtgg ggaagaccgc gaacagcagg accgccacca agagcacggt
     2341 gacgatcagc ccggcgatgg cccggcggcg ggccttggcc ttcccccgcg gtgccatcta
     2401 gcccttcgcc ccctggttga acgcgctccg gccgaggaac gcagcagcgt cgccgagccc
     2461 gtcttcgatg cgcaggagct ggttgtactt ggcgacccgg tcgctgcggg cgggagcacc
     2521 ggtcttgatc tggccgcagt tggtggcgac ggcgaggtcg gcgatggtcg cgtcttcggt
     2581 ctcgccggag cggtgcgaca tcacggcggt gtagcggttg cggttcgcca ggctcacggc
     2641 ctccagcgtc tcggtgagcg tgccgatctg gttgaccttg atgaggatcg agttggcgct
     2701 gccggcctcg atgccccggg ccaggcgggt ggtgttggtg acgaacaggt cgtcgcccac
     2761 cagctggacc cggtcgccga tcttggcggt gagcgccgcc cagccgtccc agtcctcctc
     2821 ggccatgccg tcctcgatgg acacgatcgg gtagcggtcg cagaggtcgg cccagtagtc
     2881 ggccagctcc gcgccggaga gggtgcggcc ctcgccctcg aggacgtagg cgccgtcctg
     2941 gaagacttcg gacgaggccg gatccagggc gatggcgatg tcggtgccgg gcgtgaagcc
     3001 ggcctgctcg atggcctgga ccagcagctt cagcggctcc tcgttgtcgg ccaagtcggg
     3061 ggcgaagccc ccctcgtcac ccacggcggt ggacaggccc ttgtcgtgga ggaccatctt
     3121 gagcacgtgg tacgtctcgg tgccccactg cagcgcctcg gagaagctgg ccgcgcccac
     3181 gggcatgacc atgaactcct ggaagtcgac gttgttgtcg gcgtgggcgc cgccgttgag
     3241 gacgttcatc atcggcacgg gcaggacgtg ggcgttggtg cctccgacgt ggcggtacag
     3301 cggcacgtcg agctcgatgg cggcggcttt ggcgatggcg agcgaggcgc cgaggatggc
     3361 gttggcgccg aggcgggcct tgttgtcggt gccgtcgagc tcg
//
LOCUS       DATMFS010000308         4962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105223, whole genome shotgun sequence.
ACCESSION   DATMFS010000308 DATMFS010000000
VERSION     DATMFS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105223"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VNQ33_05640"
     CDS             complement(<1..185)
                     /locus_tag="VNQ33_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97622.1"
                     /translation="MGDRFDVDALIGELTLEEKASLVSGSGFWWTRRVERVGIPSIMP
                     SDGPHGLRKQADRSDHVG"
     gene            complement(303..3566)
                     /gene="icmF"
                     /locus_tag="VNQ33_05645"
     CDS             complement(303..3566)
                     /gene="icmF"
                     /locus_tag="VNQ33_05645"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF045497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HWJ97623.1"
                     /translation="MSTPDLHHPANAVRFVTAASLFDGHDASINIMRRILQSQGVEVI
                     HLGHNRSVDEVVTACVQEDVQGVAISSYQGGHVEYFRYLVDLLAERGAGHVKVFGGGG
                     GVIVKAEIDELHAYGVSRIFSPHDGQSMGLPGMINSLVQAADVDLARDLPESLDGLLG
                     GDHAALARIITAIENDRLPSVQVAELRAAAGLRSVPVLGITGTGGSGKSSLTDELVRR
                     FRLDHEDKLQIAVIAVDPTRRKGGGALLGDRIRMNSIDPGTVFFRSLATRTAGTVVPE
                     GTDDIIAAAKAWGADLVIVETPGIGQGDAAIVDHVDCSLYVMTPEFGAASQLEKIDML
                     DFADVVAINKFDRRGGEDALRDVRRQMVRNREAFGSAPDDMAVFGTVASRFNDDGVTG
                     LYQELRSRLTGLGLATGSDDGVLPKVDTKVSSADTAIVPPARARYLADIATAVRDHHA
                     KVADQAGVARELQQVRTVRSLVDAAGGDVDAAKLPGLEAAADQRLGAEARDLLAGWPQ
                     TVADYSGDEHIVEIRGRELHTQLAKESLSGTKVRRVALPRFTDHGELLRFLMLENLPG
                     RFPFTAGVFPFKRDGERPARMFAGEGDAFRTNKRFHLLSEGQPATRLSTAFDSVTLYG
                     FDPDLRPDIYGKVGNSGVSIATLDDMKVLFDGFDLCDPSTSVSMTINGPAPTILAMFL
                     NTAIDQRLDRFRAEEGREPTDDEAAELRAWVLANVRGTVQADILKEDQGQNTCIFSTE
                     FALGMMADIAEWFVAEQVRNFYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEAYLA
                     RGMDIDDFAPNLSFFFSNGMDPEYTVLGRVARRIWAVAMRDRYGANERSQKLKYHVQT
                     SGRSLHAQEMAFNDIRTTLQALIAIYDNCNSLHTNAYDEAVTTPTEESVRRALAIQLV
                     INEEWGLAMNENPNQGSFVIDELTDLVEAAVLAEFDRIAERGGVLGAMETGYQRGRIQ
                     DESLLYETRKHDGTLPLIGVNTFLPPASADGGDDEHVEIELARSTDDEKQSQLRRLEA
                     FHAAHADERPAALARLQDAALAGDNLFAVLMDAVRSCSLGDITHTLFEVGGRYRRNI"
     gene            3641..4144
                     /locus_tag="VNQ33_05650"
     CDS             3641..4144
                     /locus_tag="VNQ33_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ97624.1"
                     /translation="MARPLPFDPIEEAGRQWDVQGWDERAAMQAATSIMRAQQIVLSR
                     VDEALRPWSLTFARYEVLVLLHFSKEGVLPLGKMGDRLMLHQASITNLVDRLEQQGLV
                     KRVPHPTDRRTTLAELTPEGRSTVADATKAVVAAQVGVQELTHREAHDLHRILKKLRA
                     GAADFEA"
     gene            4248..>4962
                     /locus_tag="VNQ33_05655"
     CDS             4248..>4962
                     /locus_tag="VNQ33_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97625.1"
                     /translation="MTPPDTTGDDPAPPPVVTGPAAPLQVGGAASALIGLLFAIPLIG
                     ALVSSSSIWPSIGPPVVALAAALARIIVRSRKQQAVQARARAALASDAPPAPVRLQVA
                     WGGSPSKVRTRFLLHRSDTGQEIGPAPVSSPDGPFDPRGQLWAYGVLEPGASIALRGA
                     DDRAALLSVAPIASTAAGEPMVVHPSSDAINRLLGWDGDPSVPVAPLPAPLAAAAWGT
                     IHRQRRAVAGPLALLAVPAV"
BASE COUNT          681 a         1655 c         1847 g          779 t
ORIGIN      
        1 ccgacgtggt cgctgcggtc ggcctgcttg cgcagcccgt ggggaccgtc ggacggcatg
       61 atcgacggga tccccacccg ctcgacccgc cgggtccacc agaacccgga ccccgacacg
      121 agcgacgcct tctcctccag cgtcagctca ccgatgagtg cgtcaacatc gaaccgatca
      181 cccatgcagg gatggctacc accccaaacc caaccgaact tttcgtgacc gtggtggctc
      241 tgccaccaac ttcacgaaaa gttcgagggg gggagggggg aggggggagg gggggtgcgg
      301 gtctagatgt tccttcggta gcggccgccg acctcgaaga gggtgtgggt gatgtcgccc
      361 aacgagcagg accgcacggc gtccatgagg accgcgaaga ggttgtcgcc ggccagcgcc
      421 gcgtcctgga ggcgggcgag ggcggcgggg cgctcgtcgg cgtgggcggc gtggaacgcc
      481 tcgagccggc gcagctggct ctgcttctcg tcgtcggtgg agcgggccag ctcgatctcg
      541 acgtgctcgt cgtccccgcc gtcggccgac gccgggggga ggaacgtgtt gacgccgatg
      601 agcggcaggg tgccgtcgtg cttgcgggtc tcgtacagca gcgactcgtc ttggatgcgg
      661 ccccgctggt agccggtctc catggcgccg aggaccccgc ctcgctcggc gatgcggtcg
      721 aactcggcca ggacggccgc ctcgaccaga tcggtgagct cgtcgatgac gaacgagccc
      781 tggttcgggt tctcgttcat ggccaggccc cactcttcgt tgatcacgag ctggatggcc
      841 agggcccggc gcaccgactc ctccgtgggg gtggtgacgg cttcgtcgta ggcgttggtg
      901 tgcagcgagt tgcagttgtc gtagatggcg atgagggcct ggagcgtggt gcggatgtcg
      961 ttgaacgcca tctcctgggc gtgcagcgat cgaccgctgg tctgcacgtg gtacttgagc
     1021 ttctggctgc gctcgttggc gccgtagcgg tcgcgcatgg cgacggccca gatgcggcgg
     1081 gccacccggc cgagcaccgt gtactcgggg tccatcccgt tgctgaagaa gaacgagagg
     1141 ttgggggcga agtcgtcgat gtccatgccc cgggccaggt aggcctcgac gtaggtgaac
     1201 ccgttggaga gggtgaaggc gagctggctg atggggttcg cgcccgcttc ggcgatgtgg
     1261 tagccggaga tggagacgct gtagaagttg cggacctgct cggccacgaa ccactcggcg
     1321 atgtcggcca tcatcccgag ggcgaactcg gtggagaaga tgcaggtgtt ctggccctgg
     1381 tcctccttga ggatgtcggc ctgcacggtg ccgcggacgt tggcgaggac ccaggcccgc
     1441 agctcggcgg cctcgtcgtc ggtgggctcc cggccctcct cggcccgaaa gcggtcgagg
     1501 cgctggtcga tggccgtgtt gaggaacatg gcgaggatgg tgggggcggg accgttgatg
     1561 gtcatcgaca ccgaggtgct cgggtcgcac aggtcgaacc cgtcgaagag caccttcatg
     1621 tcgtcgaggg tggcgatgct cacgccggag ttgccgacct tgccgtagat gtcgggccgg
     1681 aggtcggggt cgaagccgta gagggtgacg ctgtcgaacg ccgtcgacag ccgggtggcg
     1741 ggctggccct ccgacagcag gtggaagcgc ttgttggtgc ggaaggcgtc gccctcgccg
     1801 gcgaacatgc gcgccggccg ctctccgtcg cgcttgaacg ggaacacgcc agcggtgaac
     1861 gggaaccggc cgggcaggtt ctccagcatc aggaaccgga gcagctcgcc gtgatcggtg
     1921 aagcggggga gggcaacccg gcggaccttg gtgcccgaca gcgactcctt ggcgagctgg
     1981 gtgtggagct ccctgccgcg gatctccacg atgtgctcgt cgcccgagta gtcggccacg
     2041 gtctgcggcc aacccgcgag caggtcgcgg gcctcagcac cgaggcgttg atcggcggcg
     2101 gcctcgaggc ccggcagctt ggcggcgtcg acgtcgccgc ccgcggcatc cacgagcgag
     2161 cggaccgtgc gcacctgctg cagctcgcgg gcgacgccgg cctggtcggc caccttggcg
     2221 tggtggtccc gcaccgccgt cgcaatgtca gccaggtagc gggcacgcgc gggcgggacg
     2281 atggcggtgt cggcggagga gaccttggtg tcgaccttcg ggagcacgcc atcgtcgctg
     2341 ccggttgcca gcccgagccc ggtcagccgt gagcgcagct cctggtacag gccggtcacg
     2401 ccgtcgtcgt tgaagcggct ggcgacggtg ccgaagaccg ccatgtcgtc gggcgccgag
     2461 ccgaacgcct cgcggttgcg gaccatctgg cggcggacgt cgcgcaaggc gtcctcgccg
     2521 ccgcggcggt cgaacttgtt gatggccacg acgtcggcga agtcgagcat gtcgatcttc
     2581 tcgagctggc tggcggcacc gaactcgggg gtcatcacat agagggagca gtcgacgtgg
     2641 tcgacgatgg cggcgtcacc ctgtccgatg ccgggggtct cgacgatgac caggtcggcg
     2701 ccccaggcct tggccgcagc gatgatgtcg tcggtgccct cgggcacgac ggtgccggcg
     2761 gtgcgggtgg cgagcgaccg gaagaagacg gtcccggggt cgatcgagtt catgcggatg
     2821 cggtcgccca ggagggcgcc gccgcccttg cgccgggtgg ggtcgacggc gatgaccgcg
     2881 atctggagct tgtcctcgtg gtcgaggcgg aagcggcgga ccagctcgtc ggtgagcgag
     2941 gacttgcccg acccgccggt gccggtgatg ccgaggaccg ggacgctccg cagcccggcc
     3001 gcggctcgca gctcggcgac ctggaccgag ggcagccggt cgttctcgat ggccgtgatg
     3061 atccgagcca gcgcggcgtg gtcaccgccg agcagtccgt cgagcgactc gggcaggtcc
     3121 cgggccaggt ccacgtcggc ggcctggacg agcgagttga tcatgccggg gaggcccatg
     3181 gactggccgt cgtggggcga gaagatgcgg ctgacgccgt aggcgtggag ctcgtcgatc
     3241 tcggccttca cgatgacgcc gccgcccccg ccgaacacct tgacgtggcc ggcgccccgc
     3301 tcggccagca ggtccacgag gtacctgaag tactcgacgt ggccgccctg gtaggagctg
     3361 atggcgacgc cctggacgtc ttcttgcacg cacgcggtga cgacctcgtc gaccgagcgg
     3421 ttgtggccga ggtggatgac ctccacgccc tggctctgga ggatgcggcg catgatgttg
     3481 atcgacgcgt cgtggccgtc gaacaggctg gccgcggtga cgaaccggac ggcgttggcg
     3541 gggtggtgca ggtcgggcgt gctcacgtcg acagaatact ttgacttcca actatctgtc
     3601 tactgacctt cggaggacgt agcgagtacc gtgtccggcc atggcgcgcc cgctcccgtt
     3661 cgaccccatc gaggaggccg gccgccagtg ggacgtccag ggctgggacg agcgggcggc
     3721 catgcaggcg gccacgtcga tcatgcgggc gcagcagatc gtgctgtccc gggtggacga
     3781 ggccctgcgc ccgtggtcgc tcaccttcgc ccgctacgag gtgctggtcc tcctccactt
     3841 ctcgaaggag ggcgtgctgc ccctcggcaa gatgggcgac cggctgatgc tccaccaggc
     3901 cagcatcacc aacctggtgg accggctgga gcagcagggc ctggtgaagc gggtgccgca
     3961 ccccaccgat cgccgcacca cgctggccga gctcaccccc gagggccgat cgacggtggc
     4021 cgacgccacc aaggccgtgg tcgccgccca ggtcggggtc caggagctca cccaccggga
     4081 ggcccacgac ctccaccgca tcttgaagaa gctccgcgcc ggggccgccg acttcgaagc
     4141 ctgacggcgt ctcgtcgggc cgcgatcccg atcggctcag gttggcccgg gttgctgccg
     4201 atcaggtgag gatgcaccac ggcggtcccc cagcaccctt cggccccgtg acgccgccgg
     4261 acaccacggg cgacgatccc gcacctccgc cggtcgtgac cggccccgcc gccccgctcc
     4321 aggtcggtgg cgcggcctcg gccctgatcg gcctgctgtt cgccatcccg ctcatcggtg
     4381 ccctggtcag ctcgtcgtcg atctggccgt cgatcggccc accggtcgtc gcgttggcgg
     4441 cggcgctggc gaggatcatc gtccgcagcc gcaagcagca ggcggtgcag gcccgggcgc
     4501 gcgccgccct cgcctcggac gcgccgccgg ctccggtgcg tctccaggtg gcgtggggcg
     4561 gatcgccctc gaaggtgcgc acccggttcc tactccaccg atcggacacg ggccaggaga
     4621 tcggccctgc tcccgtctct tcgcccgacg ggccgttcga tccgcggggg cagctctggg
     4681 cctacggggt gctcgaacct ggggcgtcga tcgcgctccg cggtgcggac gatcgggcgg
     4741 cgctcctgtc ggtcgcgccg atcgcctcca cggcagcggg tgaaccgatg gtggtccacc
     4801 cgtcgtcgga tgccatcaac cgcctgctcg gctgggacgg cgatccgtcg gtcccggtgg
     4861 cgccacttcc ggccccgctc gctgctgccg cctgggggac catccaccgc cagcggcgcg
     4921 cggtggccgg gccgctcgcc ctgctcgccg tcccggcggt ct
//
LOCUS       DATMFS010000309         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105229, whole genome shotgun sequence.
ACCESSION   DATMFS010000309 DATMFS010000000
VERSION     DATMFS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105229"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VNQ33_05660"
     CDS             <1..1512
                     /locus_tag="VNQ33_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ97626.1"
                     /translation="RVQEMVRAGVEDLEPIGTGYRLRLVGAKTARGRRSQFVLLTATG
                     GPLDPVAGLDEWLAVRGDHDGPLFCSVHHGRRGGEAGAALSDNEISAVIGDLAVAAGL
                     PPTVSGYSLRRSWATHAYLRDPADLTAISLHLRHTSVDMTVRYIDDLGLHLLDASDYL
                     SRDAVRASGPARVARPKDLGFDEASLEDLGREVRLLTEETRPRAKSTKLADDSMWNGW
                     LQWAEGHSLDPVPASPESLVLFAAARAVDGISASTLRAQLRTIRQRHEDQGFDASDLT
                     TMADEIATGLARSAPRERTKAPVLSFGEMLDLAAVASQQAEASGSLEARRDLLMVTVG
                     YAGALRVDDLHRARVELIEELPYGLALRFATSKENPLGTANESVLLLGRDDILDPVAA
                     LTAWVDATGFSSGPLIPVLSDGPAVPLSKDSIPDRLRALATEAGITTRPTGHTLRRSW
                     ATHAYAAGVDPVTLSRHLRHRDLTTTLGYVQALSPWRDNAAAMLMANSPDRDC"
     gene            1512..>2310
                     /locus_tag="VNQ33_05665"
     CDS             1512..>2310
                     /locus_tag="VNQ33_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97627.1"
                     /translation="MDLDAQQPTSTHTPATLLCAGHTRFGKACTNQVRTPGAFCGRCT
                     GSRLTPNAAPVAEAADAPPPAERPNLRPPVAVPASTSLNEWTVANELIARLTPFAGGD
                     SELLSLIEVGARLAENSRAENTKASYRQHYRTFETFGATYGLDTTPPVDPAVVAYFVA
                     YLEHAGRLDRATGERTGEPLSHGYVRQAISAIGHRHIINGLDNPTDDPIVRGILDGHA
                     KQHGVDQDGKDPIRGADLARIITTLSQPHPTGARDLAICLLATHPDLH"
BASE COUNT          360 a          882 c          743 g          325 t
ORIGIN      
        1 cgggtccagg agatggtgcg ggcgggcgta gaggatctgg agccgatcgg gaccgggtac
       61 cggctgcggc tggtcggggc caagactgct cggggccgca ggtcgcagtt cgtgctgctc
      121 acggcgacgg gaggtccgct cgatccggtg gctgggctcg acgagtggct cgcggtccgc
      181 ggggaccacg acggtccgtt gttctgctcc gttcatcacg ggcgccgtgg aggcgaggct
      241 ggggcggcgt tgagcgacaa cgagatctcg gcggtcattg gcgatcttgc agtggccgcc
      301 gggctgccgc cgacggtgag cggctactcg ctgcggcggt cctgggccac gcacgcctac
      361 ctccgggacc cggccgatct gacggcgatc tcgctgcatc tgcgccacac gtcggtggac
      421 atgacggtcc gctacatcga cgatctcggc ctccacctcc tcgacgcctc cgactacctg
      481 tcacgcgacg ccgtccgggc atctgggccg gccagggtgg cccgccccaa ggacctcggt
      541 ttcgatgaag ccagcctcga ggacctgggc cgtgaggtcc ggttgctgac cgaagagacc
      601 cggccacgag cgaagtcgac gaagctggcc gacgattcga tgtggaacgg ctggttgcag
      661 tgggccgagg gccactcgct cgacccggtt ccggcatcgc cggagagcct ggtcctgttc
      721 gctgccgcac gggcggtcga tgggatcagc gcctcgacgc tacgagcgca gctccggacc
      781 atccgccagc gccacgagga ccagggcttc gacgccagcg acctgacgac gatggctgac
      841 gagatcgcca ccggtctcgc acgctccgca ccacgggagc gcacgaaggc cccggtgctc
      901 tccttcggcg agatgctcga cttggcagcc gttgcctcac agcaggctga ggcgtcgggc
      961 tcgctcgaag cccgacggga tctgctgatg gtcaccgtcg gctacgccgg ggcgctccgg
     1021 gtggacgatc tgcaccgggc ccgcgtcgag ctcatcgagg agctgcccta cggcctcgct
     1081 ctgcgcttcg ccacctcgaa ggagaacccc ctgggcacgg cgaacgagtc tgtgctcctg
     1141 ctgggccggg acgacatctt ggatcccgtc gccgccctga ccgcgtgggt cgatgcaacc
     1201 gggttctcct ccggcccgtt gatcccggtg ctcagcgacg ggccggctgt cccgctgtcg
     1261 aaggactcga tccccgaccg gctccgggcg ctcgccaccg aagcgggcat cacgaccagg
     1321 ccgaccgggc acacgctgcg ccggtcgtgg gccacccacg cctatgcggc gggggtcgac
     1381 ccggtgaccc tgtcacggca cctgcgccac cgcgatctga ccacgacgct cggctatgtg
     1441 caggccctgt caccgtggcg tgacaacgcc gctgcgatgc tcatggccaa cagcccggac
     1501 cgggactgct gatggacctc gacgcccaac agcccaccag cacccatacg cccgcgactt
     1561 tgctgtgcgc cggacacacc cgcttcggca aggcgtgcac caaccaggtc cgcacccccg
     1621 gcgccttctg cggccgctgc accggcagcc gtctgacccc gaacgccgca ccggtggccg
     1681 aggctgccga cgcgccgcca ccggccgagc gccccaacct gcggccacca gtcgcggtcc
     1741 ccgccagcac ctcgttgaac gagtggaccg tcgccaacga gctcatcgca cggctcacgc
     1801 cgttcgcagg gggcgactcc gagctgctgt cgctcatcga ggtcggtgcc cgactggccg
     1861 agaacagccg tgccgagaac accaaggcgt cctaccgcca gcactaccgg accttcgaga
     1921 ccttcggcgc cacctacggc ctcgacacca cacccccagt cgacccagcc gtcgtcgcgt
     1981 acttcgtcgc ctacctcgag cacgccgggc gcctcgaccg ggccaccggt gaacggaccg
     2041 gcgaaccgct ctcgcacggc tacgtccgcc aggcaatctc ggcgatcggc caccgccaca
     2101 tcatcaacgg cctcgacaac cccaccgacg accccatcgt gcgcggcatc ctcgacggcc
     2161 acgccaaaca gcacggcgtc gaccaagacg gcaaagaccc gatccgcggc gctgacctcg
     2221 cccgcatcat caccaccctc agccagcccc accccactgg cgcacgagac ctcgccatct
     2281 gcctgctcgc cacacacccc gatctgcacc
//
LOCUS       DATMFS010000310         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_172171, whole genome shotgun sequence.
ACCESSION   DATMFS010000310 DATMFS010000000
VERSION     DATMFS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172171"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNQ33_05670"
     CDS             complement(<1..469)
                     /locus_tag="VNQ33_05670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ97628.1"
                     /translation="MTRLLLVRHGESIWNADGRWQGQADPPLSERGQEQAAAAAASIG
                     TVDVLVTSDLERAADTGAIIGRILGIEPVLVEPRLRERDAGSLSGLTRPEIHEAFPGL
                     LPDDPNGFAPGPDGQPSWPHDWESDDGLWARVEVALLALARLVPDGDVVAVTHG"
     gene            555..797
                     /locus_tag="VNQ33_05675"
     CDS             555..797
                     /locus_tag="VNQ33_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWJ97629.1"
                     /translation="MAVLRLFAAARTAAGTARDLVPGETVAEILASATERYGEGFAAV
                     LPTCAVWVNGEPADGHDPVAAADEVAVLPPVSGGAS"
     gene            1028..1954
                     /locus_tag="VNQ33_05680"
     CDS             1028..1954
                     /locus_tag="VNQ33_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97630.1"
                     /translation="MTIKTPAPVPPRKGGDAPETHTGRLRRIRANRPPVDDLLPKQDD
                     HRSPFRRRYGIPYDTQGPKVRLGVLWAVAVVAVLVPQALRPWGLAVLYGVVAGAAAAQ
                     IIDAHRGNRTSADRAVAAFGASGLAVAATLGTQALGIGYLLLVAAALAAALGTPEAGR
                     LPIPRAGHTVAAAGICGGAAAALVLLADYEIGALIILLVFVMVYDASDFIVGSGASNS
                     FEGPFAGMLFIAAASAIFAVVSAPPFNDHGSDIWSFSVLAMIACPAGQILASALLPKA
                     DAHAPALRRLDSMLVVAPAWAGLIGLYLARGV"
BASE COUNT          229 a          824 c          734 g          260 t
ORIGIN      
        1 cgccgtgggt gacggccacg acgtcgccgt cgggcaccag ccgggcgagg gcgaggagcg
       61 cgacctccac ccgggcccag aggccgtcgt cggactccca gtcgtgcggc cacgacggct
      121 ggccgtcggg acccggggcg aacccgttcg gatcgtccgg cagcaggccg gggaacgcct
      181 cgtggatctc gggccgggtg aggcccgaca gcgatccggc gtcgcgctcg cgcagccggg
      241 gctccacgag gacgggttcg atgcccaaga tgcggccgat gatggcgccg gtgtcggcgg
      301 cccgctcgag gtcggaggtc accagcacgt ccaccgtccc gatcgacgcc gccgcggccg
      361 ccgcctgctc ctggccgcgc tccgagagcg gcgggtcggc ctgcccctgc cagcgcccgt
      421 cggcgttcca gatcgattca ccgtggcgga cgaggagcag tcgggtcatg gcccggccac
      481 cgtaccgggt gggggtggcg gcgctgcccc ggatgggtcg ccaccgcacc ccaccggtag
      541 gcttccgatc cgccatggcc gtcctccggc tcttcgcagc tgcccgcact gccgccggca
      601 ccgcccggga cctcgtgccg ggcgagacgg tggccgagat cctcgcttcc gccaccgagc
      661 ggtacggcga ggggttcgcc gcggtcttgc ccacgtgcgc cgtgtgggtg aacggcgagc
      721 cggccgacgg gcacgacccg gtggccgcgg ccgacgaggt cgcggtcctg ccgccggtct
      781 ccgggggcgc gtcgtgaccg acgccgacca gcctgcgggc ccccgccgcc ccccgcgccc
      841 gccccggccc ggcgacggtg cagccgcggc ggttcgaccg gcaccatcgc gcccggcccc
      901 gtcgcccggc cccagccgac ctcccgcggc gcgcaccagg cgcccgatcc cggagccgcc
      961 tggtccgcgt cccgagtcca gggcccggac cccgcgcccc aagacgccgg tcgctcctcc
     1021 caccggggtg accatcaaga cgcccgcacc cgtcccgcca cgcaaggggg gcgacgcgcc
     1081 ggagacccac accggtcgcc tgcgccgcat ccgggccaac cggccgccgg tcgacgacct
     1141 cctccccaag caggacgacc accggtcgcc gttccgccgc cgctacggca tcccgtacga
     1201 cacccagggg ccgaaggtgc gcctcggggt gctgtgggcc gtcgccgtgg tcgctgtcct
     1261 cgtcccgcag gcgctgcggc cctggggcct cgccgtgctc tacggggtgg tcgcgggcgc
     1321 ggcggcggcg cagatcatcg atgcgcaccg gggcaaccgc accagcgccg accgggccgt
     1381 cgctgcgttc ggcgcctccg gcctggccgt ggcggccacg ctgggcaccc aagcgctggg
     1441 catcggctac ctcctgctcg tcgccgcggc cctcgccgct gcgctcggca ccccggaggc
     1501 cggtcgcctg cccatcccgc gggccgggca cacggtggcc gcggccggca tctgcggcgg
     1561 tgcggccgcc gcgctcgtgc tgctggccga ctacgagatc ggcgccttga tcatcctgct
     1621 ggtgttcgtg atggtctacg acgcgagcga cttcatcgtc ggctccggcg cgagcaacag
     1681 cttcgagggt cccttcgccg ggatgctctt catcgcggcg gcgtcggcga tcttcgccgt
     1741 cgtcagcgcc ccgccgttca acgaccacgg cagcgacatc tggagcttct cggtgctggc
     1801 gatgatcgcc tgcccggcgg gtcagatcct ggcgtcggcc ctgctcccga aggccgacgc
     1861 ccacgccccc gcgctccggc gcctcgactc catgctggtc gtcgccccgg cgtgggccgg
     1921 cctcatcggc ctctacctgg cccgcggagt ctgaccacgc ggcgaggccg ccgcagtaca
     1981 gtcggtcaca tcggccgacg ggccgttcac cgcccaggag gtgacatgga caccgagctc
     2041 gatcgga
//
LOCUS       DATMFS010000311         2581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_11681, whole genome shotgun sequence.
ACCESSION   DATMFS010000311 DATMFS010000000
VERSION     DATMFS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11681"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1693)
                     /locus_tag="VNQ33_05685"
     CDS             complement(242..1693)
                     /locus_tag="VNQ33_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ97631.1"
                     /translation="MSDLAALDATAQAELVRSGEVTAAELVQAAVDRAEQVNGQLNAI
                     IHPRYQEAVAEAGADLPDGPFTGVPFVLKDLDGTAAGQPLHAGTRFLKEHGYVADTDS
                     ELTVRFRNAGLVVIGRTNTPELGLVTTTEPEAYGPSRNPWDLDRSTGGSSGGSAACVA
                     AGIVPVAHAGDGGGSIRIPAAECGLVGLKPTRGRSSLGPELGEAWAGLVTRLAVTRSV
                     RDTAALLDAVAGPGVGDPYWAPAPARPYVDELTAAPVPLKVGWTNRSFDGSIEVDPQV
                     AAATEATANLLEQLGHQVIEASPAALAAGDTLEYFLPAYSSWVARELDRLAELVGAPL
                     TEDGFEAGTWAIAEAGRATTAQQYLAALDGLHRLTRATVAWWETDGFDVLLTPTLPEL
                     PPTLGQFGATKEEPLTGLFRSAIPAHFTAPFNVTGQPGISLPLHQSTEGLPIGMQFVG
                     APAREDVLIRLAAALEAAHPWADRRPLIWTGTI"
     gene            complement(1828..>2581)
                     /locus_tag="VNQ33_05690"
     CDS             complement(1828..>2581)
                     /locus_tag="VNQ33_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWJ97632.1"
                     /translation="VIEMAQASGLLLVGGAEGNDPIAASTAGTGELIAEALDLGARRI
                     FVGHGGSATTDGGLAALKALYPAARLRGIELVAAVDVRCPFLDAAVQFAPQKGATPSQ
                     VELLSRRLERLAQVYLEDHGIDVTAIEGGGAAGGLAGGLAAVGATVESGFELIAEEQD
                     LTTAVEEADLVVTGEGFLDEASFDGKVVGGLVGLADEFGVPVLAVAGEVFDGVGDRVE
                     AVSLVQVFGRERAMADTTACITEAVTAYLATR"
BASE COUNT          364 a          928 c          961 g          328 t
ORIGIN      
        1 gtggtgccgg tcgggagccc cgaacaggtg gcctcgaccg gcgtcggtcc gtcagcggat
       61 gcgatcccgg gtacgacgag cgcgaccccc gaaccgatca tgagtgccga cagtgcggcc
      121 gcgagcgtgc gacgtgaacc cattggtctc cccccggagc gttgtcccct tgcggccacg
      181 gaccgtacca gcggtcggac aggtgtgcag gaaccgggcc ggtcccggtg aaaaccccag
      241 ctcagatggt gccggtccag atgagcggcc ggcggtcggc ccacggatgc gccgcttcga
      301 gcgccgcggc gaggcggatc agcacgtctt cacgagcggg agcccccacg aactgcatgc
      361 cgatgggcag gccctcggtg ctctggtgga gcggcagcga gatcccgggc tgaccggtga
      421 cgttgaacgg cgccgtgaag tgggccggga tcgccgagcg gaacagcccg gtgagcggtt
      481 cctccttggt ggcgccgaac tgaccgaggg tcgggggcag ctcggggagc gtgggcgtca
      541 ggaggacgtc gaagccgtcg gtctcccacc acgccaccgt ggcacgggtg aggcggtgga
      601 ggccgtcgag ggcggcgagg tactgctggg cggtggtggc ccggcccgcc tcggcgatgg
      661 cccacgtgcc ggcctcgaac ccgtcttcgg tcagcggcgc gccgaccagc tcggcgaggc
      721 ggtccagctc ccgggcgacc cacgaggagt aggccggcag gaagtactcg agcgtgtcgc
      781 cagcggccag cgcggcgggc gaggcctcga tgacctggtg gcccaactgc tccagcaggt
      841 tggcggtggc ctcggtggcc gcggccacct gcgggtcgac ctcgatcgac ccgtcgaagg
      901 accggttcgt ccagccgacc ttgaggggga ccggtgcggc cgtcagctcg tcgacgtagg
      961 gacgcgccgg ggccggcgcc cagtagggat cgcccacgcc ggggccggcg acggcatcga
     1021 gcagggccgc ggtgtcacgc accgaccggg tgacggcgag gcgggtgacg agcccggccc
     1081 acgcctcccc cagctccgga ccgagcgacg agcggccccg agtgggcttg aggccgacca
     1141 ggccgcactc ggcggccggg atgcggatgg agccgccgcc gtcaccggca tgggccaccg
     1201 gcacgatgcc agccgctacg cacgccgccg agccgccgct cgacccaccc gtgctgcggt
     1261 cgaggtccca cgggttgcgc gacgggccgt acgcctcggg ctcggtggtg gtgaccagtc
     1321 cgagctccgg cgtgttggtg cggccgatga cgaccaggcc ggcgttgcgg aagcggacgg
     1381 tgagctccga gtcggtatcg gcgacgtacc cgtgctcctt caggaaccgg gtgccggcgt
     1441 gcagcggctg tcccgcggcc gtgccgtcga ggtccttcaa cacgaacggc acgcccgtga
     1501 acgggccgtc ggggaggtcg gcaccggcct cggcgaccgc ctcctggtag cgcgggtgga
     1561 tgatggcgtt gagctgcccg ttcacctgct cggcccggtc gaccgcggcc tgcaccagct
     1621 cggccgccgt cacctccccg gagcggacga gttcggcttg ggcggtggca tcgagcgcag
     1681 caagatccga catggccgca ccctacgtcc gccagatcgc acggtccgcc ccgcccaaac
     1741 cgacgttggt gtggttgttg acggaggaca tcaaaaacca caccaagttc ggacgggcct
     1801 tgctcgggtg tcgggccccg acccgggtca gcgggtggcg aggtaggcgg tgacggcttc
     1861 ggtgatgcac gcggtggtgt cggccatcgc tcgctcgcgg ccgaacacct ggacgagcga
     1921 cacggcctcg acgcggtcgc cgacgccgtc gaacacctcg ccggccaccg cgaggacggg
     1981 cacgccgaac tcgtccgcca agccgaccag gccgcccacc accttgccgt cgaacgaggc
     2041 ctcgtcgagg aagccctcac ccgtcaccac gaggtccgcc tcctcgaccg cggtcgtgag
     2101 gtcctgctcc tcggcgatca gctcgaaccc cgactcgacc gtggccccca ccgcggcgag
     2161 gccgccggcc aggccgcccg ccgctccccc accctcgatg gcggtgacgt cgatgccgtg
     2221 gtcctccagg tacacctggg cgagccgttc gaggcgccgg gagagcagct ccacctggct
     2281 gggagtcgcc cccttctgcg gagcgaactg gacggcggca tcgaggaacg gacagcgcac
     2341 gtccaccgcg gcgaccagct cgatgcctcg cagccgggcc gccgggtaga gcgccttgag
     2401 cgcggcgagc ccgccgtcgg tggtggccga cccgccgtgg cccacgaaga tccgccgggc
     2461 gccgaggtcg agcgcctcgg cgatcagctc acccgtcccc gcggtggagg cggcgatggg
     2521 gtcgttgccc tcggcgccgc ccaccagcag gagccccgac gcctgggcca tctcgatcac
     2581 g
//
LOCUS       DATMFS010000312         5512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_313738, whole genome shotgun sequence.
ACCESSION   DATMFS010000312 DATMFS010000000
VERSION     DATMFS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313738"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VNQ33_05695"
     CDS             complement(<1..326)
                     /locus_tag="VNQ33_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97633.1"
                     /translation="MKPRSIRSALLAAAAAAAGLVVVSATGSPAGAATVSQKLDCGIG
                     GTQTVGLITTAPATVAPGGTFTVVLQPNGSGKADGAEIKNMTTTFVAPTGSQIVAGSA
                     TTSGGSG"
     gene            complement(555..2753)
                     /locus_tag="VNQ33_05700"
     CDS             complement(555..2753)
                     /locus_tag="VNQ33_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97634.1"
                     /translation="MAIATTGSTPASAAVVSQGLSCGLGGNQTSSLSTTAAATIEAGG
                     TFQVTLAPSGPAGKADGAEIKNMVTTFNVPSNASIVAGSASASGGSGTLGAYSTSISG
                     TTVKLTVPGPIANGASFVNPTLKFNLKATGAAGGTIKTTFRQSAAYTLTAAGSINISC
                     DATTPLTTLTSTTIEAVATTTSTTTTTAPTTTTTTKPGATTTSTTAPATTTTTAPPTT
                     STSAWSPTGPCGTVQTVVAPANTQSVQITATGGTGGRSGSQASSATVAGGSGGQAAAT
                     FAASAGQTFSAIVGCNGVNGGSFANTATPGGYAAGGGTGRGTIIAGQSGASGGSGGGA
                     SGACLGAVCAPGSGTPLVVAGGGGGGGVSNCAGTSSGAGGSGGSGSSTASGSGAGNSG
                     TNGSSASSSGGAGGVNSNGGSAAGGSNGNGSGGAGVNVVGGAGGGGYVGGAAGSNTSA
                     GCKGAGGGGGGSSWIRSTGTQTSFSTASSPAVTLTFTIVTPAPTTTTTQPTTTTTTAA
                     PCVADKAPFPTVQALVTQQFQDFTAKAPTSAQNTQWVSGITSCTTTADALIVSLLPTD
                     QTQDDARLVRLYLAYFKRPPDPDGFAYWQRQLDAGRGLINAAKKFAESPEFKRTYGPL
                     SNADFIELVYQNVLGRGSDPTGKAFWLTRLNNKTKNRGDVMINFSESSENIRNKVNHV
                     QVFRLHRGMMQKFPSKTTYFDLLNPILNNGKTLEDAAKAIRLSAPYDARV"
     gene            3022..3942
                     /locus_tag="VNQ33_05705"
     CDS             3022..3942
                     /locus_tag="VNQ33_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HWJ97635.1"
                     /translation="MAPTGRPAIATAPVTAGWIDAAVIAGGAELVAPEAATAVVWTQA
                     DDPDGLAALLAANPQIDWVQLPWAGIEPYVPLLGRDRTWTCAKGVYADPVAEHALALL
                     LAGFRHLHAYARAGAWTEQVGRNLLGASVTIFGGGGIAESLVRLLVPFRCTITVVRNT
                     PAPIGGVERVLGPDDRMAALAGADAVVLALPLLPSTEGLIGPEELAAMESHAWLVNVA
                     RGAHVQTDALVDALRTGAVGGAALDVTDPEPLPAGHPLWSLPNAVITPHTGNTKAMAR
                     PLLGARITENVRRYAAGEPLIGLVDVDAGY"
     gene            3980..5425
                     /locus_tag="VNQ33_05710"
     CDS             3980..5425
                     /locus_tag="VNQ33_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ97636.1"
                     /translation="MDFRRHDIEDLAGQVRSGGLSSRELTQHALDRIDATNHEVGAFV
                     AVDAERALAEAADIDARVASGDELGPLAGVPIGVKDTEDAIGFRTTKGSLLFAGSEVA
                     TADSVLVDRLRQAGCVIVGKVNTPELAWKADTDNRVFGRTGNPWSLERSAGGSSGGSS
                     AAVAAGMVPLATGSDGGGSLRIPGSLCGLTTMKPSLGRVPAGGPTPPGWADLSSKGVL
                     VHTAKESAYVLDAVIGPDPTDIRALPMPDESWRDAVENLHAPRRVVWSPTLGYAVLDP
                     EIREVCEAAVRKLEARGTEVIVIDDVFDEDSAVPWLMLSLTANLRVVEGAVTAGHGSW
                     DDLDPALRSMLEWAKATASPTTALESSDTAHHLNLRLVELFHQAPLLLTPTVAGHTPV
                     GADDGTVDGVQDIAWVRYTYPFNLTRSPAGSVCAGFTADGMPVGLQVVGPQHADVAVL
                     RLLALLEETIDLDHRCPYEPGRLGAGGADRM"
BASE COUNT          696 a         1925 c         2052 g          839 t
ORIGIN      
        1 ccgctgccgc cggaggtggt ggcggagccg gcgacgatct ggctgccggt gggcgccacg
       61 aacgtcgtgg tcatgttctt gatctcggcc ccgtcggcct tgcccgagcc gttgggctgg
      121 agcaccaccg tgaacgtgcc gccgggcgcc accgtggccg gcgcggtggt gatgagcccc
      181 accgtctggg taccgccgat gccgcagtcg agcttctggc tcacggtggc ggcgccagcc
      241 ggcgaacccg tggcgctcac caccacgagt ccggcagcag cggcggcggc tgccagcagg
      301 gcggaacgga tcgaacgggg cttcatcgca gcgtggtctc caacgtcgtg gtcagcagct
      361 cggctcccgt cagccgatcc agtgggctcg attctgacac accgtcagaa tcgaatgcga
      421 gtcagtactc ggatctggag aagggtccga gtactggctc ggctgatggc gagacccggg
      481 tgacgtgtcc gggcgcggca gcggccgggc ttggggcccg gccgctgctg tcgtggttgc
      541 cgtgtggggc gggttcagac gcgggcgtcg tagggggcgc tgaggcggat ggccttggcg
      601 gcgtcttcga gggtcttgcc gttgttgagg atggggttga gcaggtcgaa gtaggtggtc
      661 ttggagggga acttctgcat catgccgcgg tggaggcgga agacctggac gtggttgacc
      721 ttgttgcgga tgttctcgga ggattccgag aagttgatca tgacgtcgcc gcggttcttg
      781 gtcttgttgt tgaggcgggt gagccagaag gccttgccgg tggggtccga gccgcggccg
      841 aggacgttct ggtagacgag ctcgatgaag tcggcgttgg agagggggcc gtaggtgcgc
      901 ttgaactcgg gggactcggc gaacttcttg gcggcgttga tgaggccgcg gccggcgtcg
      961 agctggcgct gccagtaggc gaagccgtcg gggtccggtg gccgcttgaa gtaggcgagg
     1021 tagaggcgca cgagccgggc gtcgtcttgg gtctggtcgg tgggcagcag gctcacgatc
     1081 agcgcatcag ccgtcgtcgt gcagctggtg atgcccgaca cccactgggt gttctgcgcc
     1141 gaggtcggcg ccttggcggt gaagtcctgg aactgctggg tcaccagcgc ctgcaccgtg
     1201 gggaacggag ccttgtcggc gacgcagggg gccgccgtcg tggtcgtcgt cgtcggctgc
     1261 gtcgtggtgg tggtcggagc cggggtcacg atcgtgaagg tgagggtcac cgccggcgag
     1321 gaggccgtgg agaacgaggt ctgggtcccg gtcgagcgga tccacgagga cccgccgccg
     1381 ccaccgcccg cgcccttgca gccggcgctg gtgttgctgc cggcggcacc gccgacgtag
     1441 cccccgccgc ccgcaccgcc gacgacgttg accccggcac cgccggagcc gttgccgttg
     1501 ctgccgccgg ccgccgagcc gccgttgctg ttgacaccgc cggcgccacc gctgctgctg
     1561 gcgctcgaac cgttggtgcc cgagttgccg gcgcccgatc cgctggccgt cgacgagccg
     1621 gagccgccgc tgccgcccgc gccggacgag gtcccggcgc agttgctcac accgccgccg
     1681 ccaccgccac cggcgaccac gagcggggtg cccgaaccgg gggcgcagac cgctccgagg
     1741 caggcgccgc tggcgccgcc gccgctaccg ccggacgcgc cggactggcc ggcgatgatc
     1801 gtgccccggc ccgtgccgcc acctgcggcg tagccgccgg gcgtcgccgt gttggcgaag
     1861 ctgccgccgt tgacgccgtt gcagccgacg atggcggaga aggtctggcc ggcgctggcg
     1921 gcgaaggtgg cagcggcctg gccgccgctg ccgccggcca ccgtggcgct gctggcctgc
     1981 gacccgctgc gcccgccggt gccgccggtg gcggtgatct ggacggactg cgtgttcgcc
     2041 ggggcgacga cggtctggac cgtgccgcag ggaccggtgg gagaccaggc ggatgtcgac
     2101 gtggtcggcg gagccgtcgt ggtggtcgtg gccggtgccg tggtcgaggt ggtcgtggcg
     2161 ccgggcttgg tggtggtcgt ggtggtcggc gccgtcgtcg tcgtggtcga cgtcgtggtg
     2221 gcgaccgcct cgatggtggt gctggtcagc gtcgtgagcg gggtggtggc gtcgcacgag
     2281 atgttgatcg agcccgcggc cgtcagggtg taggcggcgc tttggcggaa ggtcgtcttg
     2341 atggtgccgc ccgcggcgcc cgtggccttc aggttgaact tgagcgtcgg gttcacgaac
     2401 gaggcgccgt tcgcgatggg cccgggcacg gtcagcttga cggtggtgcc gctgatggag
     2461 gtggagtagg cgccgagggt gccgctgccg ccgctggccg aggccgagcc ggccacgatc
     2521 gaggcgttgc tcggcacgtt gaacgtggtg accatgttct tgatctcggc accgtcggcc
     2581 ttgccggcgg ggccggacgg ggcgagggtc acctggaagg tgccgcccgc ctcgatcgtg
     2641 gccgcggctg tggtcgagag gctcgacgtc tggttgccgc cgaggccgca ggagagcccc
     2701 tggctcacca cggcggcgct ggccggcgtg ctcccggtgg tggcgatcgc caacccggca
     2761 gctgcggcgg ctgtcgccaa caggatggaa cggacagagc gtcgtttcat tgcgtgcagg
     2821 tccccttggt gttgtcgtcg cgtggaccgc ctggatcccc gagccacgcc atcgagttga
     2881 aagtctgaca caccgtcaga aaagagtcga atgttccctc ggttcttcgc tggcgctgcc
     2941 gaacagcacc tcccggcacc ccccccgcac tcagaggggg gcggcggtcg gtgccggagc
     3001 cggcacggta ccgtccgggg catggctccg accggtcgtc ccgcgatcgc caccgctccg
     3061 gtcaccgctg gttggatcga cgcggcggtg atcgccgggg gcgccgagct ggtggccccc
     3121 gaggcggcga ccgccgtggt ctggacccag gcagacgatc ccgacggcct cgcggcgctt
     3181 ctcgccgcca accctcagat cgactgggtg cagctgccgt gggcggggat cgagccgtac
     3241 gtcccgctgc tcggccgcga tcgcacgtgg acctgcgcga agggcgtcta cgccgacccg
     3301 gtggccgagc acgcgctggc cctcctgctc gctggcttcc gccacctcca cgcctacgcc
     3361 cgcgccggcg cctggaccga gcaggtcggg cgcaacctcc tcggcgcgtc ggtgacgatc
     3421 ttcggcggcg gcggcatcgc cgagtcgctc gttcgcctgc tcgtgccgtt ccgctgcacg
     3481 atcaccgtcg tccgcaacac gccggctccg atcggtggcg tcgagcgcgt gctcgggccc
     3541 gacgaccgga tggccgccct cgccggtgcc gacgcggtcg tgctggccct cccgctcctg
     3601 cccagcaccg agggcctcat cgggcccgag gagctggcgg cgatggagtc ccacgcttgg
     3661 ctcgtgaacg tggcccgcgg cgcacacgtc cagaccgatg cgctcgtcga cgcgctgcgg
     3721 accggggccg tcggcggtgc cgccctcgac gtgaccgacc cggagccgct tcccgccggc
     3781 caccccctct ggtcgttgcc gaacgcggtc atcaccccgc acaccggcaa cacgaaggcg
     3841 atggcccgcc cgctgctcgg cgcccgcatc accgagaacg tgcggcgcta cgcggcgggg
     3901 gagccgctca tcggcctcgt cgacgtcgac gccgggtact gacggcaggc agggaccacc
     3961 agacgtaggg tcctcgccca tggacttccg acgccacgac atcgaggacc tggccgggca
     4021 ggtgcgatcg ggcggcctgt cgtcacgaga gctgacccag cacgcgctcg accgcatcga
     4081 cgccaccaac cacgaggtcg gcgcgttcgt cgccgtcgac gccgagcggg cgctggccga
     4141 ggccgccgac atcgacgccc gggtcgcctc gggcgacgag ctcggccccc tcgccggcgt
     4201 gcccatcggg gtgaaagaca ccgaagacgc catcggcttc cgcaccacca agggctccct
     4261 cctcttcgcc gggagcgagg tggcgacggc cgactccgtt ctcgtcgacc gcctccgcca
     4321 ggccgggtgc gtcatcgtcg gcaaggtcaa cacccccgag ctggcgtgga aggccgacac
     4381 cgacaaccgg gtgttcgggc gcaccggcaa cccctggtcg ctcgagcggt ccgcgggcgg
     4441 gtcgtccggt ggcagctcgg cggcggtggc cgccggcatg gtccccttgg cgaccggctc
     4501 cgacggcggc ggttcgctgc ggatcccggg ctcgctctgc ggtctcacca ccatgaagcc
     4561 gtcgctcggg cgcgtccccg cgggcggccc gaccccgccg ggatgggccg acctctcgag
     4621 caagggcgtc ctcgtccaca ccgccaagga gagcgcctac gtcctcgatg ccgtgatcgg
     4681 tcccgacccg accgacatcc gagcgctccc gatgcccgac gagtcctggc gcgacgccgt
     4741 cgagaacctc cacgcaccgc gcagggtcgt gtggtcgccg acgctcggct acgccgtgct
     4801 cgacccggag atccgcgagg tctgcgaggc cgctgtccgc aagctcgagg cgcgcggcac
     4861 cgaggtgatc gtgatcgacg acgtcttcga cgaggactcg gccgtcccgt ggctgatgct
     4921 gtcgctcacc gccaacctcc gggtggtcga gggcgccgtc accgccggcc acggctcctg
     4981 ggacgacctc gacccggccc tgcgctcgat gctcgagtgg gcgaaggcga ccgcgagccc
     5041 caccaccgca ctcgaatcgt cggacacggc ccaccacctg aacctgcgcc tggtcgagct
     5101 gttccaccag gcaccgctct tgctgacgcc caccgtcgcc ggccacacgc cggtgggcgc
     5161 cgacgacggc accgtcgacg gcgtccaaga catcgcatgg gttcgctaca cgtacccctt
     5221 caacctcacc cgctcgcccg cgggatcggt ctgcgccggc ttcaccgctg acgggatgcc
     5281 cgtcggcctc caggtcgtcg gcccccagca cgcggatgtc gccgtcctcc gcctcctcgc
     5341 cctcctcgag gagacgatcg acctcgatca ccgctgcccc tacgaacccg ggaggctcgg
     5401 agcgggggga gcggatcgga tgtgagcagg tcggaggggc tgcgctaggg tccatccccc
     5461 gaacacgggc gtatagctca gctggctaga gcactgcctt cacacggcag gg
//
LOCUS       DATMFS010000313         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80931, whole genome shotgun sequence.
ACCESSION   DATMFS010000313 DATMFS010000000
VERSION     DATMFS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80931"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VNQ33_05715"
     CDS             complement(<1..894)
                     /locus_tag="VNQ33_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97637.1"
                     /translation="MTPRSATPPPRASVVARFGPLAVILLALGAVAWMSSTGTGSVST
                     AAGPGTTSKAGSANASLPVYYQDAEEEGDLDQYDFGPDCDPTTGRIKVPSLYGPPCAA
                     PRPDVKRNTSGQGVTDDTITIVYYVAADDDLSASLNAQLDRPEDAEDTASKLIAMYEK
                     TFDMWGRKLKVIRMKGSGSDETSARADARKVADEYKAFASIGGPGQEGSYAEELANRG
                     VICVGCGLSLPDSAFQQNAPYLWGNLQTPEQYLLNLGDFIIERLQGQKAGFAGSPEIR
                     KQTRNFGVVHFEQDPPVFGGVE"
     gene            complement(891..3908)
                     /locus_tag="VNQ33_05720"
     CDS             complement(891..3908)
                     /locus_tag="VNQ33_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97638.1"
                     /translation="MTATAVPEATPPEAAPEEHAGSRWRPGWLGTAGWYPLLILFGLN
                     MTDELDRSAYYLLLPEIRDSLHLTNGAILSVVALASAVALLLTVPIAHLADRRSRVAL
                     ALIGAVVWAFFSLGTGLAFAVWMLIVARSGSAIGQGVVFPTHNSLLADYYPIRSRPKV
                     FSIHRAANSAGQILGFLIAAVLTKWFSWRAPFIVFAFPTLILVVLGLKLRDPGRGHFE
                     REAANLDIDTAVDEPPPSFAEALRMVWTIRSLRRIFVALPFLAASIIGFISLASLMYE
                     EVFGLDVGERAWALIPVQLVELVGVVIGARLTTRALTRGASHIFGVLGAASAVACVTA
                     LVFAAAPNVVIAVAANCVIAASLVVVGPGVLSSLSLAIPPRARSMGFSIGALWVLPGL
                     LALPLIGWIGEHHGFRWGMAVMVPIFLIGGLVIASVGKVIDDDIAQVWTGAATRAQMM
                     QERAAGKLPLLSVRKVQVAYGDVRVLFGVDIDVAEGEIIALLGTNGAGKSTLLKAVSG
                     VAQADRGAIVFDGRDITHAPPEEIAPLGIAQVPGGQGVFPSLTVAENLRAAGWMLRHD
                     SALRAERVEQALDLFPVLRTRADDAAADLSGGQQQMLALAMAFLSEPKLLMIDELSLG
                     LAPVVVEQLLGVVRSLRDRGTTIILVEQSVNVALTVADTAYFMEKGEIRFHGPTAELL
                     DRPDVLRSVFFSGAASGLAGGDPASTSTSTSTPAVSANGDAAPVGAAVEGPGLRVEGL
                     SVAFGGIRAVDDVSFAVAPREVVGLIGPNGAGKTTVLDLISGFTRPDTGRVQLGGVDL
                     TSARPHRRAALGLGRSFQDSRLFPSLTVEETLLVALERWLDVRDPFNAALRLPPFVDS
                     ETAARHRVDELIDVLGLEAFRSKFVGELSTGSRRVVDIGCVLAHEPSVVLLDEPSSGI
                     AQREAEALAPLLLRIRDALDASLVVIEHDMSLISSVSDRLVALDQGRVVTIGTPGEVL
                     AHPEVVASYLGGDRAVLTRSGPASGAAAPPEVP"
BASE COUNT          643 a         1346 c         1566 g          499 t
ORIGIN      
        1 ctcgaccccg ccgaacaccg gcgggtcctg ctcgaagtgg acgacgccga agttgcgggt
       61 ctgcttgcgg atctcggggc tgccggcgaa cccggccttc tggccctgga ggcgctcgat
      121 gatgaagtcg cccaggttga gcaggtactg ctcgggcgtc tggaggttgc cccacaggta
      181 cggggcgttc tgctggaacg ccgagtccgg cagcgacagc ccgcagccca cgcagatgac
      241 gccgcggttg gccagctcct cggcgtacga gccctcttgg ccgggaccgc cgatcgacgc
      301 gaaggccttg tactcgtcgg ccaccttgcg ggcgtcggcg cgagccgatg tctcgtcgga
      361 gcccgacccc ttcatgcgga tcaccttgag cttgcggccc cacatgtcga aggtcttctc
      421 gtacatggcg atgagcttgg aggcggtgtc ctcggcgtct tcgggacggt ccagctgggc
      481 gttgaggctg gcggacaggt cgtcgtcggc ggcgacgtag tacacgatcg tgatggtgtc
      541 gtcggtgacg ccctggccgc tggtgttgcg cttcacatcc gggcgagggg cggcgcacgg
      601 cggaccgtag agcgacggca ccttgatccg gccggtggtg gggtcgcagt cggggccgaa
      661 gtcgtactgg tcgaggtcgc cctcctcctc ggcgtcttgg tagtagacgg gcagcgaggc
      721 gttggccgag ccggccttgc tcgtggtccc ggggccagca gcggtgctca ccgagccggt
      781 gccggtggag gacatccagg cgacggcgcc gagcgccagg aggatgacgg cgagcggtcc
      841 gaagcgggcg acgaccgaag cccgaggcgg aggcgtggcg gaacgtgggg tcatgggacc
      901 tccggagggg cagcggcgcc cgaggcgggg ccggagcggg tgaggacggc gcggtcgccg
      961 ccgaggtagg acgcgacgac ctcgggatgg gcgaggacct cgcccggggt gccgatcgtg
     1021 acgacgcggc cctggtcgag ggcgacgagg cggtccgaga ccgacgagat gaggctcatg
     1081 tcgtgctcga tgacgacgag gctggcatcg agggcatcgc ggatccgcag caggagcggg
     1141 gccagggcct cggcttcgcg ctgggcgatg cccgacgacg gctcatcgag cagcacgacg
     1201 gacggctcgt gggccagcac gcagccgatg tcgacgacgc ggcgcgagcc ggtggacagc
     1261 tcgccgacga acttggagcg gaaggcctcc aggccgagca cgtcgatcag ctcgtcgacc
     1321 cggtggcggg ccgccgtctc gctgtcgacg aacggcggga ggcggagggc ggcgttgaac
     1381 gggtcgcgga cgtcgagcca gcgctcgagg gcgacgagca gcgtctcctc gaccgtgagc
     1441 gacgggaaca ggcgggagtc ctggaagctg cgacccaggc cgagggcggc ccggcggtgg
     1501 ggccgggcgc tggtgaggtc gacaccgccg agctgcaccc tcccggtgtc gggccgggtg
     1561 aacccggaga tcaggtccag cacggtggtc ttgccggcgc cgttgggccc gatgaggccc
     1621 acgacctcac ggggagcgac cgcgaacgac acgtcgtcca cggctcggat cccgccgaac
     1681 gcgaccgaca gcccctcgac ccggagcccg ggtccctcga ccgcagcgcc gacgggagcg
     1741 gcgtcgccgt tggccgacac cgccggggtc gacgtcgagg tcgaggtcga ggccgggtcg
     1801 ccgccggcga gacccgaagc ggcaccggag aagaacacgg agcgcaagac gtcgggccgg
     1861 tcgagcagct cggccgtggg cccgtggaac cggatctcgc ccttctccat gaagtaggcg
     1921 gtgtcggcca cggtgagcgc cacgttcacc gactgctcca ccaggatgat cgtggtcccg
     1981 cggtcccgca ggctgcgcac gaccccgagc agctgctcga ccaccaccgg cgccaggccg
     2041 agcgacagct cgtcgatcat gagcagcttc ggctcggaca ggaacgccat cgccaaggcc
     2101 agcatctgct gctgcccacc ggagaggtcg gcggcggcat cgtcagcgcg ggtgcgcagg
     2161 accgggaaca gatccagcgc ctgctcgacc cgttcggccc ggagcgccga gtcgtggcgg
     2221 agcatccagc cggcggcccg caggttctcg gcgacggtga gcgacgggaa cacgccctgg
     2281 ccaccgggga cctgggcgat gccgagcggg gcgatctcct cgggtggggc gtgggtgatg
     2341 tcccgcccgt cgaacacgat ggcgccgcgg tcggcctggg ccacgccgga gacggccttg
     2401 aggagggtgg acttgccagc cccgttggtg ccgagcaggg cgatgatctc gccctcggcc
     2461 acgtcgatgt cgaccccgaa cagcacccgc acgtcgccgt aggccacctg caccttgcgc
     2521 acgctgagca gcgggagctt ccctgcggcg cgctcctgca tcatctgggc tcgggtggcg
     2581 gcgccggtcc agacctgggc gatgtcgtcg tcgatgacct tgccgaccga ggcgatgacc
     2641 aggccgccga tcaggaagat cgggaccatg accgccatcc cccaccggaa gccgtggtgc
     2701 tcgccgatcc acccgatgag cgggagggcg agcagcccgg ggaggaccca cagcgcgccg
     2761 atggagaagc ccatcgaccg ggctcggggc gggatggcga gcgacagcga cgacagcacg
     2821 cccggcccga cgacgacgag cgaggcggcg atcacgcagt tggcggccac ggcgatgacc
     2881 acgttcggag cggcggcgaa gaccagcgcc gtcacgcagg cgacggcgct ggcggcaccc
     2941 agcaccccga agatgtggga cgcgcccctg gtgagcgccc gggtggtgag ccgggcgccg
     3001 atgaccacgc ccaccagctc caccagctgg accgggatga gcgcccacgc ccgctccccg
     3061 acatcaagcc cgaagacctc ctcgtacatg agcgaggcga gcgagatgaa cccgatgatc
     3121 gaggcggcga ggaacgggag cgccacgaag atgcgccgca ggctgcggat ggtccacacc
     3181 atgcgcaggg cctcggcgaa cgagggcggc ggctcgtcga ccgcggtgtc gatgtccagg
     3241 ttcgccgcct cgcgctcgaa gtggccccgg cccggatccc ggagcttgag gccgagcacc
     3301 acgaggatga gcgtcgggaa ggcgaacacg atgaacggcg cccgccagga gaaccacttg
     3361 gtcaagacgg cggcgatgag gaagccgagg atctggcccg cgctgttggc agcgcggtgg
     3421 atcgagaaca ccttcgggcg cgaccggatc gggtagtagt cggccagcag gctgttgtgc
     3481 gtggggaaca ccacgccctg gccgatggcc gagccggagc gggccacgat cagcatccag
     3541 acggcgaagg ccaggccggt gccgagcgag aagaaggccc acaccacggc gccgatgagg
     3601 gcgagggcga cgcggctgcg gcggtcggcc aggtgggcga tgggcacggt gagcaggagg
     3661 gcgacggcgc tggccagggc gacgacgctg aggatggcgc cgttggtgag gtgcagggag
     3721 tcccggatct cgggtagcag caggtaatac gcggaccggt ccagctcgtc ggtcatgttg
     3781 aggccgaaga ggatgagcag cgggtaccag cccgcggtgc cgagccaccc cggccgccac
     3841 cgggagccgg cgtgctcctc gggcgcagcc tcgggcgggg tggcctcggg cacggccgtg
     3901 gcggtcacga cggcgacccc accggctcgt cgaccacggc gccgtcgagg ccatcgggcc
     3961 gatcgggcat cgccaccttg acggcctccg gcgcatcgtc gcccgcggtg tcggccagca
     4021 ggctcgagac cacgatcccc ttccggatgg ccag
//
LOCUS       DATMFS010000314         5160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105336, whole genome shotgun sequence.
ACCESSION   DATMFS010000314 DATMFS010000000
VERSION     DATMFS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105336"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VNQ33_05725"
     CDS             <1..818
                     /locus_tag="VNQ33_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97639.1"
                     /translation="AGKSTLLRMLCGLTPPDRGAVRILGADPRKDIAVRARIGIVPQQ
                     EAMFEALTAFEFVRLAGLLHGVGDPAGAARWALDLVELDHADHRSTTTYSKGMKQRVK
                     VAQALVNDPAIVVLDEPLTGLDPRQRGHLIELFGRLGAQGRCVIVSSHVLDEVERFGS
                     RVLVVAQGRLAATGDFRAIRDLMDDRPHRIRVRTDRLREVATELISSPGVLAVRLEPA
                     AGNRAGAVIVDTADAPLFRRSVAAAAQRADATLLEVSPLDDDLDSVFRYLVGR"
     gene            815..1579
                     /locus_tag="VNQ33_05730"
     CDS             815..1579
                     /locus_tag="VNQ33_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97640.1"
                     /translation="MTTTPSPKAPPLRSGPALGRVLLAEYRLLLRSVCTRGRLVAIAM
                     LAGLSVLTAVAVALSNPFDPLSEGVGFVNGNIATLIPVAVLVFGAAVLGDLIDDGSLV
                     YLWLRPVPSWVHVLAGWAATVTIVVPLVLVPVVGATAVIDSSPALVGAAVVGGLVAVA
                     GYAGLFVMLGVRFRRALPWGLVYILIWEGFVASAGKTATKLAVRSYVRSILGSMTDTH
                     LKLGEFSLGVGIVVPLLIAVLALLYASRRLARSDVA"
     gene            complement(1629..2234)
                     /locus_tag="VNQ33_05735"
     CDS             complement(1629..2234)
                     /locus_tag="VNQ33_05735"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ97641.1"
                     /translation="MVDIGEQERRRPGRPRDAAVDDAITDAVVELLTEVGFRGLTIDG
                     VAHRAHVGKATIYRRWPGKEQMVLDALTAGRFPVPQPDTGSLHEDLLAYYLPLADLEA
                     QKGAVRLMPALAAEAAVDAEIAERLHAYVSDRRAPVAGILRRAQERGEVADNVDVELV
                     VDLLTGAIMYRLYFSGARVDEDVIRRLLEGVLVAIGADGAR"
     gene            complement(2608..4131)
                     /gene="gatB"
                     /locus_tag="VNQ33_05740"
     CDS             complement(2608..4131)
                     /gene="gatB"
                     /locus_tag="VNQ33_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWJ97642.1"
                     /translation="MTAAPALPTDSLGNTITVAESDDVLVVEAADGSRWELVCGLEVH
                     AELATETKMFSGAPNHFGDDPNTNIDPVTLGLPGTLPVVNQQAVELAARFGCAIGATV
                     QRSVFSRKNYFYPDMPKNFQISQYDQPIVVDGRLELPSGHVVGIERAHLEEDTGKSTH
                     VGGAGRIKGAEYSLVDYNRAGVPLLEIVSRPHVRSAEQARQYVAELRSILVACAISDG
                     KMEEGSMRVDANISVRREGSGELRTRCEIKNVNSLRSLVRAIEYEARRQADLHAAGQK
                     VTQETRHWDENDGRTHTMRSKEEADDYRYFPEPDLVPVDLGAERVAEIAAALPLLPAA
                     RRHRLAGLTGEPVTAEAVVVTVERGQDDQALAAIEAGADAGRVLVHVVQNLAVDGGAD
                     LAPERLAELVKLETSGDLTATQAKQVLAELVADPSTTAAEVAKAKGFEAMDTGALESI
                     VDGVINANPAEWEQFCAGDDKARKKLSGFFTGQIMKASKGQADGRAVAQLLAQRSAS"
     gene            complement(4128..>5160)
                     /locus_tag="VNQ33_05745"
     CDS             complement(4128..>5160)
                     /locus_tag="VNQ33_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWJ97643.1"
                     /translation="NPVDLSRVPGGSSGGSAAAVAAGFAPLSLGSDTGGSIRQPAAFC
                     GVVGVKPTYGLVSRYGLIAFASSLDQVGPFAATVADAALLLEVIAGHDPADATSIPQP
                     APAISEHLDDGVAGLRVGVISELMGEGIDPVIAAQVRAAADALAAAGATVTEVSVPAV
                     TFGLSAYYLIAPAEASSNLARFDGVRYGLRVDAPTTNEMMVATRTQGFGPEVKRRIML
                     GTYALSAGYYDAFYGQAQKVRTLIARDFAAAYEEVDVLLSPTTPTTAFALGAIEDPLT
                     MYLNDTCTIPTNLAGDAAMSVPFGADAAGLPVGVQILAPALGEVPMFRAAAVVEAAAP
                     ARPTGKDLR"
BASE COUNT          647 a         1844 c         1912 g          757 t
ORIGIN      
        1 gcgccggcaa gtccaccctg ctgcgcatgc tgtgcggcct gaccccaccg gaccgcggcg
       61 ccgtgcgcat cctcggggcc gacccgcgca aggacatcgc cgtccgggct cgcatcggga
      121 tcgtgcccca gcaggaggcc atgttcgagg cgctcacggc gttcgagttc gtgcggctcg
      181 ccggcctgct ccacggggtc ggggaccccg cgggcgcggc ccgctgggcg ctcgacctgg
      241 tggagctcga ccacgccgac caccgatcca ccaccaccta ctcgaagggg atgaagcagc
      301 gggtgaaggt ggcccaggcc ctcgtgaacg atccggccat cgtggtgctc gacgagccgc
      361 tcaccggtct cgacccccgc cagcgcggcc acctgatcga gctgttcggc cgtctgggcg
      421 cgcagggccg ctgcgtcatc gtgtccagcc acgtgctcga cgaggtcgag cgcttcggtt
      481 cgcgggtcct cgtggtcgcg cagggccgcc tcgccgcgac gggcgacttc cgggcgatcc
      541 gcgacctcat ggacgaccgg ccccaccgca tccgcgtgcg caccgaccgc ctccgcgagg
      601 tcgccaccga gctgatctcg agtcccggcg tgctcgccgt ccgcctcgag ccggcagcgg
      661 gcaaccgcgc cggggccgtc atcgtggaca ccgccgatgc accgctgttc cggcgctcgg
      721 tcgcggcggc cgcgcagcgc gcggacgcca ccctgctcga ggtctcgccc ctcgacgacg
      781 acctcgacag cgtcttccgc tacctggtgg gccgatgacc accaccccgt caccaaaggc
      841 gccgcccctg cgctcgggcc ctgcgctcgg acgcgtgctc ctcgccgagt accggctcct
      901 gctccgctcg gtctgcacca ggggccgcct cgtcgccatc gccatgctgg ccgggctcag
      961 cgtcttgacc gccgtcgccg tcgccctcag caaccccttc gacccgttga gcgagggcgt
     1021 gggtttcgtc aacggcaaca tcgcgacgct catccccgtc gccgtgctcg tcttcggcgc
     1081 cgccgtcctc ggggacctca tcgacgacgg ctcgctcgtc tacctctggc tgcgccccgt
     1141 gccgtcgtgg gtccacgtcc tggccgggtg ggccgccacg gtgacgatcg tcgtcccgct
     1201 cgtgctcgtc ccggtggtgg gcgccacggc cgtcatcgac tcgtcgccgg ccctcgtggg
     1261 tgcggctgtc gtcggcggtc tcgtcgccgt cgccggctac gccgggctct tcgtcatgct
     1321 cggggtccgg ttccgtcgag cgctcccctg ggggctcgtg tacatcctca tctgggaggg
     1381 cttcgtcgcc tccgctggca agaccgccac caagctcgcc gtccgctcct acgtccggtc
     1441 gatcctcggt tcgatgaccg acacgcacct gaagctcggc gagttctccc tcggcgtcgg
     1501 catcgtcgtc ccgctcctca tcgccgtcct cgcactgctc tacgccagcc gtcgcctggc
     1561 ccgctccgac gtcgcctgac gcgcgggccg cggtggcggt tcggctcctg gcctcctggg
     1621 atctacagct agcgggcgcc gtcggcgccg atggcgacga ggacgccctc gagcaggcgg
     1681 cggatcacgt cctcgtcgac ccgtgccccg ctgaagtaga ggcggtacat gatcgcgccg
     1741 gtgagcaggt cgaccaccag ctcgacgtcg acgttgtcgg cgacctcgcc gcgctcctgg
     1801 gcccgccgga ggatcccggc cacgggcgcc cgccggtcgg agacgtacgc gtggaggcgc
     1861 tcggcgatct cggcatcgac cgccgcctcg gccgcgagcg cgggcatgag ccgcaccgca
     1921 cccttctgcg cctcgaggtc ggcgagcggc aggtagtagg cgagcaagtc ctcgtggagg
     1981 ctgcccgtgt cgggctgggg caccgggaag cggcccgccg tgagggcatc gagcaccatc
     2041 tgctccttgc cgggccaccg ccggtagatc gtggccttgc cgacgtgggc gcgatgggcg
     2101 accccgtcga tcgtgaggcc gcggaacccg acctcggtca gcagctcgac gaccgcatcc
     2161 gtgatcgcgt cgtcgaccgc cgcgtcgcgc ggccggcctg gccgcctccg ctcttgttca
     2221 ccgatgtcaa ccacccgtac cccatttcac ccgcccgccg gggcggccga cggtcgagcg
     2281 gtggcggggg gtgccatcgg ccgggtccgt caatcgacag agacggtacc gctgatctcc
     2341 gccagcgcaa gcgatcggtg gcgctcgtca accgccgggc gctcgatgtc cgaaaaaggg
     2401 ggtgccggct cgcggccgat ggggtggtgg gaggaccatc ggctgcaagc cggcaccgcg
     2461 aggggcatcc tattccagaa ccaggtagtt ccgaaaacca gccgatcacg aacggggggt
     2521 cgagccgcgg gcttggcccg ccgacggtgg gcctggaccc ggcggcgccc ggtggacgcg
     2581 ctcgccgcgg cggccgtccg cgcccgatca gctcgcggac ctctgggcga ggagctgggc
     2641 gacggcgcgg ccgtcggcct ggcccttcga cgccttcatg atctggccgg tgaagaagcc
     2701 gctcagcttc ttgcgggcct tgtcgtcacc ggcgcagaac tgctcccact cggccgggtt
     2761 ggcgttgatg acgccgtcga cgatcgactc cagcgcaccg gtgtccatcg cctcgaagcc
     2821 cttggccttg gccacctcgg ccgcggtggt ggacggatcg gccaccagct cggcgaggac
     2881 ctgcttggcc tgcgtggccg tgaggtcgcc ggaggtctcc agcttcacca gctcggccag
     2941 gcgctcgggc gccaggtcgg cgccgccgtc gacggcgagg ttctgcacga cgtggacgag
     3001 cacgcggccg gcgtcggcgc cagcctcgat ggcagcaagg gcctggtcgt cctggccgcg
     3061 ctcgacggtc acgacgacgg cctcggcggt gacgggctca cccgtgaggc cggcgaggcg
     3121 gtggcggcgg gcggccggga gcagcgggag ggcggcggcg atctcggcca cgcgctcggc
     3181 accgaggtcg acggggacga gatcgggctc ggggaagtac cggtagtcgt cggcctcctc
     3241 cttcgagcgc atggtgtgcg tgcggccgtc gttctcgtcc cagtgccggg tctcctgcgt
     3301 gaccttctgg cccgcagcgt ggaggtcggc ctggcggcgc gcctcgtact cgatggcccg
     3361 cacgagcgag cgcagcgagt tcacgttctt gatctcgcag cgggtgcgga gctcgccgct
     3421 cccctctcgg cggacggaga tgttggcgtc gacgcgcatc gacccctcct ccatcttccc
     3481 gtcggagatg gcgcaggcca cgaggatgga gcgcagctcg gcgacgtact ggcgggcctg
     3541 ctcggcggag cgcacatggg gccgcgagac gatctccagc agcgggacgc cggcgcggtt
     3601 gtagtcgacg agcgagtact cggcgccctt gatgcggccg gcgccgccca cgtgggtgga
     3661 cttgccggtg tcctcttcga ggtgcgcccg ctcgatgccg acgacgtggc ccgacggcag
     3721 ctccaaccgg ccgtcgacga cgatcggctg gtcgtactgc gagatctgga agttcttcgg
     3781 catgtccggg tagaagtagt tcttccggct gaagaccgac cgctggacgg tggcgccgat
     3841 ggcgcagccg aagcgggcgg cgagctccac cgcctgctgg ttcaccacgg gcagcgtgcc
     3901 cgggagaccg agggtcaccg ggtcgatgtt ggtgttgggg tcgtcgccga agtggttcgg
     3961 cgcgccggag aacatcttgg tctcggtggc cagctcggcg tggacctcca ggccgcacac
     4021 cagctcccag cgggagccgt cggccgcctc gacgacgagc acgtcgtcgg actcggcgac
     4081 ggtgatggtg ttgccgagcg agtccgtggg cagggcgggg gcggcggtca tcggaggtcc
     4141 ttcccggtgg ggcgggcggg ggcggcggcc tcgacgacgg cggcggcccg gaacatcggc
     4201 acctcgccga gggccggcgc caggatctgc acgccgacgg gcagcccggc ggcatcggcg
     4261 ccgaacggga ccgacatggc ggcatcgccg gccaggttcg tggggatcgt gcaggtgtcg
     4321 ttgaggtaca tcgtcagcgg gtcctcgatg gccccgaggg cgaacgccgt ggtgggcgtg
     4381 gtcggagaca ggagcacgtc gacctcctcg taggccgccg cgaagtcccg ggcgatgagg
     4441 gtgcgcacct tctgggcctg cccgtagaag gcgtcgtagt acccggccga gagggcgtag
     4501 gtgccgagca tgatgcgccg cttgacctcg gggccgaagc cctgggtccg ggtggcgacc
     4561 atcatctcgt tggtcgtcgg cgcgtcgacc cggaggccgt agcggacgcc gtcgaagcgg
     4621 gccaggttgc tggacgcctc ggccggggcg atcaggtagt aggcggacag cccgaacgtg
     4681 acggccggga ccgacacctc ggtgaccgtg gcgccggccg cggcgagggc gtcggccgcg
     4741 gcccggacct gggcggcgat gacgggatcg atgccctccc ccatcagctc ggagatgacg
     4801 ccgacgcgga ggccggcaac gccgtcatcg aggtgctcgg agatggccgg ggccggctgc
     4861 gggatggacg tggcgtcggc cgggtcgtgg ccggcgatga cctccagcag cagggcggcg
     4921 tcggccacgg tggcggcgaa ggggccgacc tggtcgagcg aggaggcgaa ggcgatgagg
     4981 ccgtaccggc tcacgaggcc gtacgtgggc ttcacgccga cgacgccgca gaacgcggcg
     5041 ggctggcgga tcgacccgcc ggtgtcggac ccgagcgaga gcggggcgaa gccggccgcc
     5101 accgcggccg cggagccgcc cgaggaaccc ccggggaccc ggctgagatc gaccgggttg
//
LOCUS       DATMFS010000315         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_80939, whole genome shotgun sequence.
ACCESSION   DATMFS010000315 DATMFS010000000
VERSION     DATMFS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80939"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="VNQ33_05750"
     CDS             complement(<1..2095)
                     /locus_tag="VNQ33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWJ97644.1"
                     /translation="MSALDAFGPRSWTRPEVTGFGREPMSTYLARPAVMSLDGEWAFL
                     LRDRPEEVAADDLTGPTDGWAAIEVPGCWTVQGFDRPQYTNIQMPFPGPPPHVPDANP
                     TGVYRRTVTLPTEWEGQRVVLYVAGAETVLYVHVDGAPVAMGKDSRLPTEVDLTGIVT
                     PGVPFELALSVVRWSDATYLEDQDHWHHAGLHRRVFAYATPPVHIADVHATAGFDPAT
                     GEGRLRTVVRLGADGHGPRDWTARIELGGQEATVAARFEHPTDSLTNWLVFDGRQAVG
                     EIVVPEAAPWSAEHPNLHDLRVTLLDGDGSEVDTVSLRVGFRQVEVVGHELLVNGRPV
                     LIKGVNRHDHDPRQGKTVTPEGIRADLVLMKQHGINAVRTSHYPNDAVLYDLCDELGL
                     YVVDEADLETHAYLRSLLKDPRWGSAVLERIARMAQRDKNHPSIIIWSLGNESGSAPI
                     LHAAAEWLRQWDGTRPVQYESSIGEAVFTDLTNGGALELGELMSRPTPESDLVVPMYP
                     EVPDLVSWATRTVPDRPLVMCEYIHAMNNSCGGLADYWDAIRTHPGLQGGFVWDWVDQ
                     ALVQQLPDGTERLAYGGDFGDEPNDGPFCLNGLVDANRVPHPALLELAAVIAPVRIAA
                     VDAARGVVRITNEYDFRDLSHLLPSWVVDVDGTAVASGELEPLDLAPGVSTTVRIPAP
                     RVDLQAGERAHLTVAF"
BASE COUNT          325 a          813 c          859 g          345 t
ORIGIN      
        1 cgaaggccac ggtgaggtgg gcgcgctccc cggcctggag gtcgacccga ggagccggga
       61 tccgaacggt ggtcgacacg cccggtgcca gatcgagcgg ctccagctcg ccggaggcca
      121 cggcggtgcc gtcgacgtcg acgacccagc tgggcagcag gtgcgacagg tcgcggaagt
      181 cgtactcgtt cgtgatccgc acgacgcccc ttgcggcgtc gaccgccgcg atgcgcaccg
      241 gagcgatcac cgcggccagc tccagcaggg ccgggtgggg gacccggttc gcatcgacga
      301 ggccgttgag gcagaacggc ccgtcgttcg gctcgtcgcc gaagtcgccg ccgtaggcga
      361 gccgctcggt gccgtcgggc agctgctgga ccagcgcttg gtccacccag tcccacacga
      421 agccgccctg gaggccgggg tgcgtgcgga tggcgtccca gtagtcggcc agcccgccgc
      481 acgagttgtt catggcgtgg atgtactcgc acatgacgag cggccggtcc ggcacggtgc
      541 gggtggccca cgacacgagg tccgggacct cggggtacat cggcaccacc aggtcggact
      601 ccggggtcgg gcggctcatg agctcaccca gctccagcgc tccgccgttc gtcaggtcgg
      661 tgaagaccgc ctcgccgatg ctgctctcgt actggaccgg ccgcgtcccg tcccactggc
      721 gcagccactc ggcggcggcg tggaggatcg gcgcgctccc gctctcgttg ccgagcgacc
      781 agatgatgat cgacgggtgg ttcttgtcgc gctgggccat gcgggcgatg cgctcgagca
      841 cggcgctccc ccacctcggg tccttgagca ggctgcgcag gtaggcgtgg gtctcgaggt
      901 cggcctcgtc gaccacgtac aggccgagct cgtcgcacag gtcgtagagc acggcgtcgt
      961 tcgggtagtg cgacgtgcgc acggcgttga tcccgtgctg cttcatcaac acgaggtcgg
     1021 cgcggatgcc ctcgggcgtc acggtcttgc cctggcgcgg gtcgtggtcg tggcggttga
     1081 cgcccttgat gagcacgggc ctcccgttca cgagcagctc gtggcccacg acctccacct
     1141 gccggaagcc cacgcgcagc gacacggtgt ccacctcgct gccgtcgccg tcgagcaggg
     1201 tcacccgaag gtcgtggagg ttcgggtgct cggcgctcca gggggcagcc tcgggcacga
     1261 cgatctcccc caccgcctgg cgcccgtcga acacgagcca gttcgtgagc gagtcggtgg
     1321 ggtgctcgaa ccgggccgcg acggtggcct cctggccgcc gagctcgatg cgcgcggtcc
     1381 agtcgcgcgg gccgtgtccg tcggcgccaa ggcggacgac cgtgcgcagc cggccctcgc
     1441 cggtggccgg atcgaagccg gccgtggcgt gcacgtcggc gatgtggacc ggcggtgtgg
     1501 cgtaggcgaa cacgcgacgg tggaggccgg cgtggtgcca gtggtcctgg tcttcgaggt
     1561 aggtggcgtc ggaccagcgc acgaccgaca gggccagctc gaagggcacg cccggcgtca
     1621 cgatgccggt cagatcgacc tcggtcggca accgggagtc cttgcccatg gcgacgggtg
     1681 cgccatcgac gtgcacgtac aggacggtct cggcgccagc gacgtacagc acgacccgct
     1741 gcccttccca ctcggtcggc agggtgaccg tgcgccggta gaccccggtc gggttggcgt
     1801 cgggcacatg gggcggcggt cccgggaacg gcatctggat gttcgtgtac tgcggccggt
     1861 cgaacccctg caccgtccaa cagcccggca cctcgatcgc ggcccagccg tccgtcgggc
     1921 cggtcaggtc gtcggccgcg acctcctcgg gccgatcccg gaggaggaac gcccactcgc
     1981 cgtcgagcga catcaccgct ggccgtgcga ggtacgtcga catcggctcc cgcccgaacc
     2041 cggtgacctc gggccgggtc cacgaacgcg gcccgaacgc gtcgagcgcg ctcaccggag
     2101 gatgggcggg gaacagacgg ccgcggtcac ctgctcaccc ttgcagacgc gctcgtccgt
     2161 ccgtccccat ctcgaagttg gtgtgcgaac tgacgctctg cgtcgtttcg cacaccaact
     2221 tcgatcaggt ggatggccgc cagaagaggg tcagctgcag ccgctggtgc tgccgcagct
     2281 ggggcaggcg tggcaggagc cagcccgctg catctggacg ccgcactggt agcagtaggg
     2341 cg
//
LOCUS       DATMFS010000316         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105386, whole genome shotgun sequence.
ACCESSION   DATMFS010000316 DATMFS010000000
VERSION     DATMFS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105386"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VNQ33_05755"
     CDS             complement(<1..897)
                     /locus_tag="VNQ33_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97645.1"
                     /translation="MLAGLVLTSLSAAMLPAALFQRKSRSGWLEELDGIREDALEQHE
                     GGVANVVDADDIWTDLRDGGEYEDPHEISSLYATDETPDRPTAKPSSRPARAARPSTE
                     APADASPPAAAAASLPPPPPPVVEAPPAPPIAEPAASAAPVGEAPAPAPEAPATGSQY
                     DDQIWVQPAPHPIRPSEMWHPAELAPAPLAEESFEPVREERVASADLMDPTYLAAADA
                     APAAAAASPAAEATAGRLESDLAARADAVIVEPVAPAGSLVNGPVGRRVRRDRPAERP
                     KASLPPPEAQQPRADRGEQSPLP"
     gene            1055..1858
                     /gene="mutM"
                     /locus_tag="VNQ33_05760"
     CDS             1055..1858
                     /gene="mutM"
                     /locus_tag="VNQ33_05760"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWJ97646.1"
                     /translation="MPELPEVETVRRGLEPVLLGRRFVDGGAHPSDKFNRALGVIDRT
                     VRSVDRRGKYLLIGLDDGRRLVIHLGMTGQFRVHEGEPPPDPHVRAWWLLDDGRTLSF
                     RDVRRFGRIAVVGTDVSALPTLAALGPEPFDPAFTAASLRDAVNRSQTRVKTQLLGQR
                     IVAGVGNIYADEALWRAQVHPASRRLTLPAAGRLRGAIIDVLAEGIDHGGTTLRDYRT
                     VEGREGGNQHRLDCYGRAGLPCPRCGTELKRSVIDGRGTTHCPACQRRS"
     gene            1887..2681
                     /locus_tag="VNQ33_05765"
     CDS             1887..2681
                     /locus_tag="VNQ33_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97647.1"
                     /translation="MTDEGAPTPLSDLFSVDGKVVVVTGGTRGIGRMIAGGFVAGGAT
                     VIVSSRKAEACRTTAEELSAYGSCTGIAADLSTEEGAKGLAEAVAALHDHVDVLVNNA
                     GATWGVPLAEHDAASWNRVLDLNVQGVFHASKFFLPLLQADASADEPSRVINIGSIDG
                     LRAPMMETFSYGASKAAVHHLTRHLAKHLAPTVTVNAIAPGPFESKMMAATLDAFGEQ
                     IAATTPMKRIGRPDDMAGTAIFLASRAGAYLTGTVIPVDGGISTTA"
BASE COUNT          374 a         1058 c          991 g          347 t
ORIGIN      
        1 gggcaggggc gactgctcgc cgcgatcggc ccggggctgc tgggcttcgg gcgggggcag
       61 cgacgccttg gggcgctcgg ccggacggtc gcgccggacg cggcggccga ccggcccgtt
      121 gacgaggctc cccgcgggcg ccaccggctc gacgatcacg gcgtcggccc gtgccgccag
      181 gtccgattcg agccggccgg ccgtcgcctc ggcggccggc gacgccgccg cggccgcggg
      241 ggcggcgtct gcggccgcca ggtaggtcgg gtccatcagg tcagccgagg ccacccgctc
      301 ctcccggacc ggctcgaacg actcctcggc caggggcgcc ggagccagtt cggccggatg
      361 ccacatctcg ctcgggcgga tgggatgcgg cgcgggctgc acccagatct ggtcgtcgta
      421 ctgcgacccg gtggccgggg cctccggtgc cggggccgga gcctcgccca ccggggccgc
      481 actggctgca ggctcggcga tcgggggtgc cggcggcgcc tccaccacgg gaggaggtgg
      541 cggcggaagg gatgcggccg ccgcggccgg cggcgacgca tcggccggag cctcggtgct
      601 cggccgggca gcgcgggccg gacgcgacga cggcttggcc gtcggacggt cgggcgtctc
      661 gtcggtggcg tacagcgagg agatctcgtg gggatcctcg tactcgccgc cgtcgcggag
      721 gtcggtccag atgtcatcgg catcgacgac gttggcgaca ccgccctcgt gctgctcgag
      781 cgcgtcctca cggatcccgt cgagctcctc gagccacccc gaccgggact tccgctggaa
      841 gagcgcggcc gggagcatcg cagccgagag cgaggtcaga accagtccag cgagcacggg
      901 gcaccatcgg cagcgctcgg ccggtcctga gcgactcggg cccagaatct ggacgttccg
      961 agccggcgca ggtcagccca ccacagaggg tgacctatct cgcctccgga ccacgctcag
     1021 cagcgttcgg ggaccgatcc ggtaccgtcg cctcgtgccc gagctgcccg aggtggagac
     1081 cgtccgtcgg ggcctggaac cggtgctcct cggccgtcgc ttcgtcgacg gaggcgccca
     1141 cccgtcggac aagttcaacc gcgccctcgg cgtcatcgac cgcaccgtcc gctcggtcga
     1201 ccgccggggc aagtacctgc tgatcgggct cgacgacggc cggcggctgg tcatccacct
     1261 cggcatgacg ggccagttcc gggtccacga gggcgaaccg ccgcctgatc cgcacgtgcg
     1321 ggcctggtgg ctgctcgacg acggccgcac cctctcgttc cgggacgtgc gccggttcgg
     1381 tcgcatcgcc gtggtgggca ccgatgtgag cgccctcccg accctggccg cgctcggccc
     1441 cgagccgttc gacccggcgt tcaccgcggc gtcgctgcgc gatgcggtga accgcagcca
     1501 gacgagggtg aagacgcagc tcctcggcca gcggatcgtc gccggtgtcg gcaacatcta
     1561 cgccgacgag gccctctggc gggcccaggt ccacccggcg agccgccgcc tcaccctgcc
     1621 cgcggccggg cggctgcgag gagcgatcat cgacgtgctg gccgagggca tcgaccacgg
     1681 cggcacgacg ctccgggact accggaccgt cgagggccgc gagggcggga accagcaccg
     1741 cctcgactgc tacggccgcg ccgggctgcc gtgcccccgc tgcggcaccg agctgaagcg
     1801 atcggtcatc gacggccggg gcaccaccca ctgccccgcc tgccagcgcc ggtcctagcc
     1861 gcagcgctgg gataccgtcg gcggccatga ccgacgaagg cgctcccacc cccctgtccg
     1921 acctcttctc cgtcgacggc aaggtcgtcg tcgtcaccgg cggcacccga gggatcgggc
     1981 gcatgatcgc gggcggcttc gtcgccggtg gcgccaccgt catcgtctcg agccgcaagg
     2041 ccgaggcctg caggaccacc gccgaggagc tgtccgccta cgggtcgtgc accggcatcg
     2101 ccgccgacct ctccaccgag gagggggcga aggggctggc cgaggccgtc gccgccctcc
     2161 acgaccacgt cgacgtgctg gtgaacaacg ccggcgccac gtggggcgtc ccgctcgcgg
     2221 agcacgacgc cgcatcgtgg aaccgggtgc tggacctcaa cgtgcagggc gtcttccacg
     2281 ccagcaagtt cttcctgccc ctcctgcagg ccgacgcctc ggccgacgag cccagccggg
     2341 tgatcaacat cggctcgatc gacggcctcc gcgccccgat gatggagacg ttctcctacg
     2401 gcgcctcgaa ggccgcggtc caccacctca cgcggcacct cgcgaagcac ctggccccga
     2461 ccgtcaccgt caacgccatc gcccccggcc cgttcgagtc gaagatgatg gcggccaccc
     2521 tcgacgcgtt cggcgagcag atcgccgcca ccaccccgat gaagcggatc ggccgtcccg
     2581 acgacatggc cgggaccgcg atcttcctgg cgtcccgggc cggggcctac ctgaccggta
     2641 cggtcatccc cgtcgacggt ggcatctcca ccaccgcata ggagggaagc agacccatga
     2701 ccgacgctga cgagcccacc ggcgcctgga cgccggccga cgacgccgac agcacccagg
     2761 cgtggacccc
//
LOCUS       DATMFS010000317         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_242470, whole genome shotgun sequence.
ACCESSION   DATMFS010000317 DATMFS010000000
VERSION     DATMFS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242470"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VNQ33_05770"
     CDS             complement(<1..159)
                     /locus_tag="VNQ33_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HWJ97648.1"
                     /translation="MNVPDDLKYSSDHEWIRVDGDRITIGITDYAQDALGDVVFVQVP
                     EQGALVAAG"
     gene            complement(239..>2327)
                     /locus_tag="VNQ33_05775"
     CDS             complement(239..>2327)
                     /locus_tag="VNQ33_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWJ97649.1"
                     /translation="VENPLEFGIVITRDDGTIERFLEKPTWGQVFSDTINTGIFVLEP
                     EIFDFIPEGRPVDFSGEVFPALLEAGKPLYGAVAEGYWEDVGTLESYVSAHKDVMDGR
                     VTVDVPGFEFGQGVWIGEGAEVHPEARIEGFAIIGENSRVEAGARLGDYTVLGGNVRV
                     RGDADLERSVVHDNAYLAEGCRIRGTVIGRASDLRRGARTDDGVVIGDECFIGDDAHI
                     GAGVKIYPFKTVEAGATINSSIIWESKGARSLFGRDGISGLANVDISPELAARVAMAF
                     ATTLRKDTTVITSRDSSRAARMLKRAFMAGLNASGVNVLDLEVAPVPVTRFLTRQPVA
                     TAGVTIRLVESDSQSVMIRFFDRDGIDITEDAQRKIERLIGREDFRRVLAGEIGDIGF
                     PPRALEQYTAALGETVDLAVVRNASFKLVIDYGYGATAFVMPNVLAKLGADVLGVNPY
                     ASTRQAITIDRADATERVSALVLASGAHLGAVLDADGEHLTLIDDTGHELTDDEARLA
                     FVSLVAGHLVGDRIALPVNTSSAADRLVEGHGVRIQRTKTSTAALMAAASERGVGFAA
                     SGDGGFILPGFLPAFDGGAALVKLLELLALEQTSLSAVRESLPRTHLAHETVITPWEQ
                     KGLVMRTLVELSKDRRLELIDGVKVIHDDGWVLALPDPEEPVTHIWAEASDTAGARAL
                     AKEYVRRIRQTLR"
BASE COUNT          330 a          766 c          870 g          361 t
ORIGIN      
        1 acccgcggcg accagggcgc cctgctcggg gacctgcacg aacacgacgt cgccgagcgc
       61 atcttgcgcg tagtcggtga tgccgatcgt gatgcggtcg ccgtcgaccc ggatccactc
      121 gtggtcgctg gagtacttca ggtcatcggg gacgttcatg agggctgagg ctatcccacg
      181 gggtcgggcc gggcggcgag cgcaaccctc aggctcaggt tcacggttgg tgccggtgtc
      241 atcggagggt ctggcggatg cggcggacgt actccttggc cagagcgcgg gctccagcgg
      301 tgtcggatgc ctcggcccag atgtgggtga ccggctcctc ggggtcgggc agggcgagca
      361 cccacccgtc gtcgtggatg accttcaccc cgtcgatcag ctcgaggcgt cggtccttgc
      421 tgagctcgac gagggtccgc atgacgaggc ccttctgctc ccagggcgtg atgacggtct
      481 cgtgggcgag gtgcgtccgg ggcagcgact cgcggacggc cgacagggag gtctgctcca
      541 gggcgagcag ctcgagcagc ttgacgagcg ccgctccccc gtcgaacgcc ggcaggaagc
      601 cggggaggat gaagcccccg tcgccgctgg cggcgaagcc gacgccgcgc tcgctggccg
      661 cggccatgag cgccgcggtg gacgtcttgg tgcgctggat gcgcaccccg tggccctcga
      721 ccagccgatc ggctgcggag ctggtgttca cgggcagtgc gatgcggtcg ccgaccaggt
      781 ggccggccac cagcgacacg aacgccaggc gggcctcgtc gtcggtgagc tcgtggccgg
      841 tgtcgtcgat gagcgtgagg tgctcgccgt cggcgtcgag cacggccccc aggtgggcgc
      901 ctgatgccaa gacgagggcg gagacccgct cggtggcgtc ggcgcggtcg atggtgatcg
      961 cctgacgggt ggatgcgtag gggttcacgc cgaggacgtc ggctcccagc ttcgccagca
     1021 cgttgggcat gacgaaggcg gtggcgccgt acccgtagtc gatgacgagc ttgaacgacg
     1081 cgttgcggac gacggcgagg tcgacggtct cccccagggc cgccgtgtac tgctcgaggg
     1141 ctcggggcgg gaagccgatg tcgccgatct cgccggccag cacgcggcgg aagtcctccc
     1201 ggccgatcag ccgctcgatc ttgcgctggg cgtcttcggt gatgtcgatg ccgtctcggt
     1261 cgaagaaccg gatcatcacc gactgcgagt cgctctcgac gaggcggatc gtgacgccgg
     1321 ccgtcgcgac gggctggcgg gtcaggaagc gggtgaccgg caccggcgcc acctcgaggt
     1381 cgagcacgtt caccccgctg gcgttgagac cggccatgaa cgcccgcttg agcatgcggg
     1441 cggcccgcga cgagtcccgg gacgtgatga cggtggtgtc cttgcgcagc gtggtggcga
     1501 acgccatggc gacgcgggca gcgagctcgg ggctgatgtc gacgttggcg agcccggaga
     1561 tgccgtcgcg gccgaagagg ctgcgagcgc ccttcgactc ccagatgatc gacgagttga
     1621 tggtggcgcc ggcctcgacg gtcttgaacg ggtagatctt cacgccagcg ccgatgtggg
     1681 cgtcgtcgcc gatgaagcac tcgtcgccga tgaccacgcc gtcgtcggtg cgagcccccc
     1741 ggcgcagatc gctggcccgg ccgatgaccg tgccgcggat gcggcagccc tcggcgaggt
     1801 aggcgttgtc gtggacgacg ctgcgctcga ggtcggcatc gccgcggacc cggacgttgc
     1861 cgccgagcac cgtgtagtcg cccaagcggg cgcccgcctc gacccggctg ttctcgccga
     1921 tgatggcgaa gccctcgatg cgggcctcgg ggtggacctc ggccccctcg ccgatccaca
     1981 cgccctggcc gaactcgaac ccgggcacgt cgacggtgac ccggccgtcc atcacgtcct
     2041 tgtgggccga cacgtaggac tcgagggtgc cgacgtcttc ccagtagccc tcggcgacgg
     2101 cgccgtacag gggcttgccg gcctcgagca gggcggggaa cacctcgccg gagaagtcga
     2161 ccgggcggcc ctcggggatg aagtcgaaga tctccggctc cagcacgaag atgccggtgt
     2221 tgatggtgtc gctgaacacc tggccccacg tcggcttctc caggaagcgc tcgatggtgc
     2281 cgtcgtcgcg cgtgatgacg atgccgaact ccaatgggtt ctcgacc
//
LOCUS       DATMFS010000318         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_172409, whole genome shotgun sequence.
ACCESSION   DATMFS010000318 DATMFS010000000
VERSION     DATMFS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172409"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VNQ33_05780"
     CDS             complement(<1..122)
                     /locus_tag="VNQ33_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWJ97650.1"
                     /translation="MSPKETTMADAISTLTQASFPEAIATADKPVLVDFWAEWCG"
     gene            complement(170..1135)
                     /gene="trxB"
                     /locus_tag="VNQ33_05785"
     CDS             complement(170..1135)
                     /gene="trxB"
                     /locus_tag="VNQ33_05785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWJ97651.1"
                     /translation="MTTRHNVVIIGSGPAGLTAAIYTARANLAPLILEGEPSSTSDQP
                     GGQLMLTTEVENFPGFPDGVMGPELMESFRNQAIRFGADARQEKVSKVDFSERPFKLW
                     VGDPDAAEPTYLADSVIVSTGAQSLMLDLPREKELLGHGVSTCATCDGFFFREHHIAV
                     VGGGDSAVEEATFLTKFADSVTLIHRRDELRASKIMQERAFSNPKIKMLWDSEVAEIH
                     GDTKLAGITVRSTTTGELEDLDVTGLFVAIGHRPNTDLFKGVLDMEDNGYLITGPGTA
                     TNIDGVFACGDVQDHTYRQAITAAGSGCMAAIDAERWLEANAHHA"
     gene            complement(1233..1580)
                     /locus_tag="VNQ33_05790"
     CDS             complement(1233..1580)
                     /locus_tag="VNQ33_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97652.1"
                     /translation="MAAPTPSPRGKVPQGIIPAEWPAQAADTIVDTISKVRDKTTKPA
                     LLAARAVVYGLVAAIVGTIAFFLLLLMIVRWYDVWVPGPVWPIYAFFAVVFLLVGLIA
                     LRKANAPRPGATA"
     gene            complement(1642..2532)
                     /locus_tag="VNQ33_05795"
     CDS             complement(1642..2532)
                     /locus_tag="VNQ33_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97653.1"
                     /translation="MTELPPLSDDDLSLALDGEADAALLARIDARPEAQARLAELRAA
                     QELLGSASVPALDDDAVDQLVATAVDTPVPPEHGRSRGQRHAAPWIVAAAVILLMAVG
                     LSLVWAGRSDDQDQASASLTSQDSTAEPSAGKAAADSSFSGAGTAASEVDTGAHGAPT
                     TTVTTVTGTAAVPVLYLGSYPTAADLRTATATSLGDAWKRSDTRLQYDGDDEESTDRT
                     NRSANPPAQAAIDRCADQLRVTLSMKAGPIQTGYATVDGKDVLVYEFATASARDADRE
                     TTLVAAVGADACDEVVIFER"
     gene            complement(2529..>2742)
                     /locus_tag="VNQ33_05800"
     CDS             complement(2529..>2742)
                     /locus_tag="VNQ33_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWJ97654.1"
                     /translation="VDAALAQLAPDFRAAVVLRDICQLDYAEIAEVLEIPAGTVRSRI
                     ARGRGQLADLLRGNQPASDGRPTPTP"
BASE COUNT          385 a          846 c         1086 g          425 t
ORIGIN      
        1 ccgcaccact cggcccagaa gtcgaccagc accggcttgt cggcggtggc gatggcctcg
       61 gggaagctag cttgggtgag ggtgctgatg gcgtctgcca tggtggtctc cttgggtgac
      121 attcgtccgg taacaaccgg ggatcagggc tggtgattcc cggtcgggct caggcgtggt
      181 gggcgttcgc ctcgagccag cgctcggcgt cgatggcggc catgcagccc gagccggcgg
      241 ccgtgatggc ctggcggtag gtgtggtcct ggacgtcgcc gcaggcgaag acgccgtcga
      301 tgttggtggc ggtgccggga ccggtgatca ggtagccgtt gtcctccatg tccaagacgc
      361 ccttgaacag gtcggtgttg ggccggtggc cgatggcgac gaacagcccg gtgacgtcga
      421 ggtcctcgag ctcgccggtg gtggtgctgc gcacggtgat gccggcgagc ttggtgtcac
      481 cgtggatctc ggccacttcg ctgtcccaga gcatcttgat cttcgggttc gagaacgctc
      541 gctcctgcat gatcttcgac gcgcggagct cgtcgcgacg gtggatcagc gtgacgctgt
      601 cggcgaactt ggtgaggaag gtcgcctcct cgaccgccga gtcgccgccg cccacgacgg
      661 cgatgtggtg ctcgcggaag aagaagccgt cgcaggtggc gcaggtggac acgccgtgac
      721 caaggagctc cttctcgcgg ggcaggtcca acatgagcga ctgggcgccg gtggagacga
      781 tgaccgagtc ggccaggtag gtgggctcgg cggcatcggg atcgccgacc cagagcttga
      841 agggccgctc ggagaagtcg accttcgaca ccttctcctg gcgggcgtca gcgccgaagc
      901 ggatggcctg gttgcggaag ctctccatga gctcgggacc catgacgccg tcggggaacc
      961 cggggaagtt ctcgacctcg gtggtgagca tcagctgccc gccgggctgg tcggaggtgg
     1021 acgagggctc gccttcgagg atgagggggg ccaggttggc gcgggccgtg tagatggcag
     1081 cggtgagccc agcgggaccc gatccgatga tgacgacgtt gtggcgagtg gtcacggaga
     1141 ctccgaggcg ttcggtggga aggttccaac gccgagaccg gtcggaagct tcccggtcgg
     1201 ctcggcggcg tccgggctcc gtcaggccga ggtcaggcgg tggcgccggg cctgggcgcg
     1261 ttcgccttgc ggagcgcgat caacccgacg agcaagaaga cgacggcgaa gaacgcgtag
     1321 atgggccaga ccggaccggg gacccacacg tcgtaccagc gcacgatcat caacagcagg
     1381 aggaagaagg cgatcgttcc gacgatggcg gcgacgagcc cgtagaccac ggcccgggct
     1441 gcgagcagcg ccggcttggt ggtcttgtcg cggaccttgc tgatcgtgtc gacgatcgtg
     1501 tcggccgcct gcgccggcca ctcggcgggg atgatgccct gggggacctt gccgcgcggt
     1561 gatggagtcg gtgcagccat ggggccagac actacccgca ggcgcgaacc gcagacccgc
     1621 ccgcaacgag cggtgacctg gtcagcgctc gaagatgacg acctcgtcgc aggcatcggc
     1681 accgacggca gcgacgaggg tggtctcgcg gtcggcgtcg cgtgccgagg cggtggcgaa
     1741 ctcgtagacg agcacgtcct tgccgtcgac ggtggcgtag ccggtctgga tgggcccggc
     1801 cttcatgctc aacgtgaccc ggagctggtc ggcgcagcgg tcgatcgccg cctgcgccgg
     1861 tgggttggcg ctccggttcg tgcggtcggt ggactcctcg tcgtcgccgt cgtactgcag
     1921 ccgcgtgtcg ctgcgcttcc aggcgtcgcc gagcgaggtg gcggtggccg tccgcaggtc
     1981 cgcggcggtc gggtacgagc cgaggtagag caccggcacc gcggcggtgc cggtgacggt
     2041 ggtgacggtg gtggtgggtg caccgtgcgc accggtgtcc acctcgcttg cggcggtgcc
     2101 ggcgccggag aaggaggagt ccgccgcggc cttccccgcg ctcggctcgg ccgtcgagtc
     2161 ctgggaggtg aggctggcgc tggcctggtc ctggtcgtcg ctgcggccgg cccacacgag
     2221 gctgaggccg acggccatga ggaggatcac cgcggcggcg acgatccacg gggcggcgtg
     2281 ccgctggccg cgcgaccgcc cgtgctcggg tggcaccggc gtgtccaccg cggtggcgac
     2341 gagctgatcg accgcgtcgt cgtcgagggc gggcaccgac gcggagccca gcagctcttg
     2401 ggcggcgcgc agctcggcga ggcgggcctg ggcctcgggg cgggcgtcga tccgcgccag
     2461 cagggcggca tcggcctcgc cgtcgagggc gagcgacagg tcgtcgtcgg agaggggtgg
     2521 cagctcggtc atggtgtggg tgtgggacgt ccgtcggagg cgggctggtt cccgcggagc
     2581 aggtcagcca gctgtcctcg gccccgtgcg atgcgagagc gcacggtacc ggcggggatc
     2641 tccaagacct cggcgatctc cgcgtagtcg agctggcaga tgtcccgaag taccacggcg
     2701 gcgcgaaaat ccggcgcaag ctgggcgagg gcggcatcga cc
//
LOCUS       DATMFS010000319         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_220386, whole genome shotgun sequence.
ACCESSION   DATMFS010000319 DATMFS010000000
VERSION     DATMFS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220386"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2343)
                     /locus_tag="VNQ33_05805"
     CDS             complement(139..2343)
                     /locus_tag="VNQ33_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ97655.1"
                     /translation="MRILIAAWRDLANDLAGGSEVLIDHLCAGMTERGHDVTLLCANP
                     IGEGHTYRVHPNGGTVDQYLRAPFAYLRNYRNVDLVVDVANGVSFYTPFWRRGASICL
                     VNHIHTEQWDQWFSWPVAPLGRTAELKAMPAVYRNRLFVAVSPSTADLLESMGVDRDR
                     IRIIINGTELPETVGAEAPEPLFVGLGRLVPHKRFELAVEAFNQIQPIIGGRLVIAGD
                     GPERQRLLELAGPGVEIPGRISEEEKEDLLRSAWLMVHPASHEGWGLVITEAAAYGTP
                     ALAFRVPGLQDSIVDGMSGILVDRPEELAERWLELAQDKPLRDRLRAGALRRAAACSW
                     DATVDRFEEICEEAVQSHRRSLQLPPGSWTGPDDRVTVDQYLGEDDGDGASPELAGIP
                     VGTGRPSLQVMRSRPDLSIVLPAFNEAGRLPVSIPVLTEHLAQRSGTTEVIVVDDGST
                     DETVRIGTELLRGLPLAGVYRLGGHAGKGAAVRAGVARATGRNIVFMDADLATDLDFL
                     DPLVASLDEVDVAIGSRSAPGAITSGVTPSSDAAHRLFNTLSRSLTGLPITDFQCGFK
                     AFRAATGRLLFHLIRERGYAFDVELLALADRLGMEIREVPIHWRAVRGSHVRIVVDSV
                     HMSYQVARIAQRSRYNQTLSAIEAYGQAADLPLEVLVASVRSQLPVAAPIIPWAKGAL
                     VLLPFVEPVDATELATAIERSLDGVLVRPTSVVASQILDAADRGLLQRFASS"
     gene            2533..>3346
                     /locus_tag="VNQ33_05810"
     CDS             2533..>3346
                     /locus_tag="VNQ33_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97656.1"
                     /translation="MPVVPTSNPALPAPERGAVDPPPGWRLPAWWPWGVGLLLGLVVL
                     GPGLSGGSLLSLDLLVTPDIPVPNGMYGLGPALSQRVPLFAVLGLGSAVIGGPAITKL
                     LIVAFLATGFAGAAHLARVLAPAGMRVGAWGQLAAGLLWAAGPYALTRTGVGHINLLW
                     VVAVLPWVLPRLCRPADHLPSTYLAALALALGGPGGGTLGIAAAAVALVVQPRPRRFV
                     RPVLAVAVPQLLWVLPTAVLLWAGAGVTGATGFPTRADGVGGWPSLLTGAGFW"
BASE COUNT          400 a         1180 c         1291 g          475 t
ORIGIN      
        1 catccaagtc ggcgacgggc gccgtcaagg tggccaccgt gaccaccacc cagtcggttc
       61 gcgggtcggg gaaccgcagc agggcgaggg cgtggcggga agccggggcg gggagggcgg
      121 cgtcgtccac ggaggtgatc agctcgaggc gaaccgctgg aggaggccgc ggtcggcggc
      181 atcgaggatc tgggatgcga cgacgctggt gggccgcacc aagacgccgt cgaggctgcg
      241 ctcgatcgcc gtggccagct cggtggcgtc gaccggctcc acgaacggga gcaagacgag
      301 cgcgcccttc gcccacggga tgatcggggc ggcgacgggc agctgcgacc ggaccgaggc
      361 gaccagcacc tcgagcggca ggtccgcggc ttggccgtac gcctcgatgg ccgagagcgt
      421 ctggttgtag cggctgcgct gggcgatgcg ggccacctgg tagctcatgt ggaccgagtc
      481 gacgacgatc cggacgtggc tgccgcgcac ggctcgccag tggatcggca cctcgcggat
      541 ctccatgccc aggcggtcgg cgagggcgag cagctcgacg tcgaacgcgt agccccgttc
      601 ccggatgagg tggaacagca ggcgtcccgt ggcggctcgg aacgccttga acccgcactg
      661 gaagtcggtg atgggcaacc cggtgagcga gcgtgagagc gtgttgaaca gccggtgggc
      721 ggcgtcgctc gacggggtca cgccgctcgt gatggccccg ggggcgctgc gcgatccgat
      781 cgcgacgtcg acctcgtcga gcgaggcgac gagcgggtcg aggaagtcca ggtcggtcgc
      841 cagatcggcg tccatgaaga cgatgttgcg tccggtggcg cgggcgacgc cggcgcgcac
      901 cgccgccccc ttcccagcgt ggccgccgag ccggtacacg ccggccaggg gcaggccgcg
      961 gagcagctcc gtgccgatgc gcaccgtctc gtcggtgctg ccgtcgtcga cgacgatcac
     1021 ctcggtggtg ccggaccgct gggccaggtg ctcggtgagg accgggatcg acaccggcag
     1081 gcgcccggcc tcgttgaacg ccggcagcac gatgctgagg tcggggcgcg agcgcatcac
     1141 ctgcagcgac gggcgtccgg tgccgaccgg gatgccggcg agctcgggcg aggcgccgtc
     1201 gccgtcgtct tcgcccaggt actggtcgac cgtcacgcgg tcgtcgggcc cggtccacga
     1261 gcccggcggc agctgcagcg agcgccggtg ggactggacc gcctcctcgc agatctcctc
     1321 gaaccggtcg acggtggcgt cccagctgca ggccgccgcc cgccggagcg caccggcgcg
     1381 cagccggtcc cggaggggct tgtcctgggc gagctcgagc caccgctcgg ccagctcctc
     1441 gggccggtcg acgaggatgc cgctcatccc gtcgacgatg gagtcctgga ggccgggcac
     1501 gcggaaggcg agcgcggggg tcccgtaggc ggcggcctcc gtgatgacga ggccccagcc
     1561 ctcgtggctg gcggggtgga ccatgagcca cgcgctgcgc agcaggtcct ccttctcctc
     1621 ctcgctgatg cggccgggga tctcgacgcc cggacccgcc agctccagga gccgctggcg
     1681 ctcggggccg tcgccggcga tgacgaggcg cccgccgatg atcggctgga tctggttgaa
     1741 cgcctccacg gccagctcga agcgcttgtg gggcacgagg cgcccgagcc cgacgaagag
     1801 cggctcgggg gcttcggcgc ccacggtctc cggcagctcc gtgccgttga tgatgatccg
     1861 gatgcggtcg cggtcgacgc ccatcgactc cagcaggtcc gcggtggacg gcgagacggc
     1921 gacgaagagg cggttccggt agacggccgg catggccttc agctcggccg tgcgcccgag
     1981 gggggcgacg ggccaggaga accactggtc ccactgctcg gtgtggatgt ggttgaccag
     2041 gcagatcgac gcgccacggc gccagaacgg cgtgtagaag gacacgccgt tggccacgtc
     2101 gaccaccagg tcgacgttgc ggtagttgcg caggtaggcg aacggcgccc gcaggtactg
     2161 gtcgacggtg ccgccgttgg ggtggacccg gtaggtgtgg ccctcgccga tcgggttggc
     2221 gcagagcagg gtgacgtcgt ggcctcgctc ggtcatgccc gcgcagaggt ggtcgatcag
     2281 gacctccgag ccgcccgcga ggtcgttcgc caggtcccgc cacgcggcga tgaggatgcg
     2341 catcaggcgc tggctccggt ccgcgccgtg atcttgggtc cttcggccgt cgatcgggtc
     2401 atggtggtcc ttgcgctggg gccgacccgg cggacgacgt cctcccggcg gcatcgtgcc
     2461 tccgcccggc gggcggagcg aacaacagta gcgtgaccgg tcgctcgcgg ccccggacgg
     2521 atcgcgtcga ggatgcccgt ggtacccacc tccaacccag cgctgccggc cccggagcgg
     2581 ggagccgtcg accccccgcc ggggtggcgg cttccggcgt ggtggccgtg gggcgtcggc
     2641 ctcctcctcg gcttggtggt gctgggcccc ggtctctccg gcggttcgct cctcagcctc
     2701 gatctgctgg tgacccccga catccccgtg cccaacggga tgtacggcct cggccccgcg
     2761 ctgtcgcagc gggtcccgct gttcgcggtg ctggggctgg gttcggccgt catcggcgga
     2821 cccgccatca cgaagctcct gatcgtcgcg ttcctcgcca cgggcttcgc gggggccgcc
     2881 cacctggctc gggtcctggc ccctgccggc atgcgggtcg gcgcgtgggg ccagctcgcc
     2941 gccgggctcc tctgggccgc cggcccgtac gccctgacgc ggaccggtgt cggccacatc
     3001 aacctgctct gggtcgtggc cgtcctgccg tgggtcctcc cgcggctgtg ccgcccggcc
     3061 gaccacctgc cgtcgaccta cctcgccgcg ctggcgctgg ccctcggcgg tcccggcggc
     3121 ggcaccctcg ggatcgccgc ggctgccgtg gcgctcgtgg tgcagccccg gccccgccgc
     3181 ttcgtgcggc ccgtgctcgc ggtggcggtg ccgcagctgc tgtgggtgct cccgaccgcg
     3241 gtcctcctct gggccggggc cggggtcacc ggtgccacgg gcttcccgac ccgggccgac
     3301 ggggtcggcg ggtggccgtc gctcctcacc ggcgccggct tctggc
//
LOCUS       DATMFS010000320         6944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_172428, whole genome shotgun sequence.
ACCESSION   DATMFS010000320 DATMFS010000000
VERSION     DATMFS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172428"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6282)
                     /locus_tag="VNQ33_05815"
     CDS             complement(<1..6282)
                     /locus_tag="VNQ33_05815"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HWJ97657.1"
                     /translation="MSFSGSAAAFSPNRSGLPYVLDSPSSGGGGGLKVLSLPWTQGVL
                     AWTDYYHFSSKSYSFTPGTADGVLSADGRTFVGKFSRQEYWSETNNSQGQQVYLRFDD
                     PYCDGVNRSYYNEGQIMVWRRSTRFGEFGDPELLTKQTIEDDPCAKNWEVELTQPWDV
                     EPFPTGATGPRKVPALGTGWGNQSQHPSVSADGSSIAFRASADGCMCGGASTGFTVDA
                     GIYISDNGGGPVPVYLNADLEPDMGQRFGNHDYGSVQVSDDGLHVLFATASTGVVPGL
                     IDPEEPIDALYMATRSAVGADDWTFELVSKDSSGDPVSAKFGSMSQNGSRVAYIQDSG
                     ASSRLALWTRDSSTASSSRYVVSPSSALTAVDIGDRPGVPSISADGTRIAIMARSYDT
                     SGELPVLTDSKLRVFDADLAASASPTDRRRASLASRQMQDNSNGGGSLAVGGSNGRFT
                     VAMQSGAPLARYGNNSGLYLIGSSEIPDPETGANHADPVEVATGSFTQHESDLSSPSG
                     TSVTAIRRDYSSLGEQSGIFGPGWATAFDMRLAFKVADGADVVSVELPDGRIVDYSAT
                     SGSWANEEGYLPVLEPDALNWTLTETDGAESTFDSAGRLISVERPGDPVVSLDWEGEG
                     SGSVTVSAGSGYSVELTDDQGWVLDEESSEYVLAAGPDGLVDRVVDSEGRQVDYGYQV
                     VSQRTVLHTVSKPHLVGQAAGTFGTRTYDVDEQLRITSIVDDVDATRQHTLVENTYET
                     DGRVAEQVSDTGDVSSFQYDLKPNPPAGLVAAPGFTTVTDAASGATTVYEYGLNGEVL
                     GVTDAAGQAISKSWEEGKPTSFEARSGATTSYTYDAAGRPLTVSETAGGSTRVTSTLT
                     YVVPDSSAAARADDRIATSTGEDGVTTNYTYDGDANEPDTVSVPCDAASLGAGLSCPS
                     SGKATTSYRYFTGSLAGLTKDEVDPDGVKTSYTYHADRSLASSTTYEDDGTPRTTTFD
                     TLHPGDSGWPFSDPLIAEASRTESPGGAVSWEGRDAQGQTRESRDPLYNGTSHKATRT
                     AYRLDGSVASVTDPADHTTTFDVHRPGDSGWSEATGIAEVRISTDADGISSINKIDRS
                     GYTVVSQTGNLSVPAELATTTNTYGDLGRLVSSTGPTGIVTSFHYDAEGRTTGTTIGP
                     DPDDAAHTFSTEYDARGQVLTKTLPLGTDPDGTAVQRKVSYTYDSAGRTVSMLEGSGG
                     PAAEQLLTGYVYDSAGRLWRTIEHRHNDTDTANPTTIHAEDRVTETRYTLGGRTAQVL
                     GPPVDVATFDWASPDAAKSVVSFSYDDAGRRVNTTAPDGGITETAYDEDGRISAVTSP
                     GGAEIHYLYDAAGHRTGVVSPSGLEGPGDPTTVTALTGYDAAGRVISETDPHIAQTGV
                     TDPSARTTTYTSAGRVATVTDANGNTVTYGYDSRGNRTSRTATDDASNPVIESWTWNL
                     ANQQLTHTVPPPRAGAPSQTSMATYDPDYGWLATATDPTSRVQTHAHYNDGTLKQTTF
                     TRSTDAAVVNKFWVNGRGWRTKSTSATGAGAAKTWTSSYDRAGQKVTETGPQGTISHS
                     YGLAGEQTSLGYPDGAAISTYLHDPAGRLTDVSVTSGGITVPYVSYSYDSDGHQTSEL
                     IHAAGGATRAWDYNDAGQLASFTEVLPNTTSTWETYTAELTYRPDGRLATETVNSDPT
                     ITYSYDDAGQLTSAVDGVRDRSWTYGTRGNRLTSVDNGVTTTYTTNPNASVAKTTEGT
                     HTVTYGYDDAGRRLTGMAKSGTTTTGTTTTSYTTEGYASTLVVAATGSTTTSEVRGYD
                     ADGKLVKSDTGGGTTVTRHYQWDTTQPVPQIIDARNSGSTWFRATWGNQRIAFQSGTA
                     APQWFQYDVRGSVIRTDTTNTDVVAGPQKYSPFGEIGVSSGADRIGYRGELTASNFVH
                     LRARNYDSVTGQFTTRDPLDGVDGTPVVADPYHYVDNDPINKIDPLGLTPVDCFFNTQ
                     DGCRYIVQDTESFTTGTRQELPSELPPAKYTINLMLADIRTGAEAGSADVRMVYAIFR
                     TELGKDPTGEWLGDTLDDFGIREEDSRGPTNFFRESWERFWSLHSTQLQAYYASTGED
                     AYGDVLTKEEGWKGLPNLPW"
     gene            complement(6579..>6944)
                     /locus_tag="VNQ33_05820"
     CDS             complement(6579..>6944)
                     /locus_tag="VNQ33_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97658.1"
                     /translation="LPYPDDVLDCVARRATEDEDLRAALEKPGAAANLAVADGAAASC
                     VIEVRAAPRFAEDLQRAAGGGLTEKQLACAVREFGRLTSAEVEAAAGAVLNPEKAEAA
                     ATAPVEEIYSVCDIDVGDR"
BASE COUNT         1023 a         2203 c         2426 g         1292 t
ORIGIN      
        1 ccagggaagg tttggaagcc ctttccatcc ctcctctttt gtgaggacat cgccgtatgc
       61 atcttccccc gtgctggcgt agtaagcttg aagttgcgtt gagtgcaacg accagaacct
      121 ctcccatgac tcgcggaaaa agttggtcgg accgcgacta tcctcctcac gaattccgaa
      181 gtcatcgagt gtatcgccga gccattcacc ggtaggatct tttccaagct cagtacggaa
      241 aatggcatag accatgcgaa cgtccgccga tccagcttcg gctccggtcc ggatgtccgc
      301 caacatgaga tttatagtgt atttggcagg tgggagttca gagggaagtt cctggcgtgt
      361 accggtagta aatgattccg tgtcctggac gatgtaccgg cagccgtctt gagtgttaaa
      421 gaagcaatca acgggagtta gtcccaacgg atcgatcttg ttgatgggat cattatcaac
      481 gtagtggtag ggatcggcta ccacgggggt cccgtcaacg ccgtcgagtg ggtcccgggt
      541 tgtgaactgg ccggtgacgg agtcgtagtt gcgggctcgg aggtggacga agttggaggc
      601 ggtgagttcg cctcggtagc cgattcggtc ggccccggag gacacgccga tctcaccgaa
      661 gggcgagtac ttctgaggtc ccgcgacaac gtcggtgttg gtggtgtcgg tgcggatgac
      721 tgagccgcgg acgtcgtact ggaaccactg gggtgctgcg gtgccgctct gaaaggcgat
      781 gcgctggttg ccccaggtgg cgcggaacca ggtggagccg gagttgcggg cgtcgatgat
      841 ctgcgggacg ggctgggtgg tgtcccactg gtagtggcgg gtgacggtgg tgccgccgcc
      901 ggtgtcggat ttgacgagct ttccgtcggc gtcgtagccg cgcacctcgg aggtcgtggt
      961 cgagccggtg gcggcaacga ccagcgtgct cgcatacccc tctgtcgtgt agctggtggt
     1021 ggtggtacct gtggtggtgg ttcccgattt ggccatccct gtgaggcgcc gtccggcgtc
     1081 gtcgtagccg taggtgacgg tgtgggtgcc ttcggtggtc ttggcgaccg atgcgttggg
     1141 gttggtggtg taggtggtgg tgacgccgtt gtcgacggag gtgagccggt tgccgcgggt
     1201 gccgtaggtc cacgaccggt cccggacgcc gtcgaccgct gaggtgagct ggccggcgtc
     1261 gtcgtagctg taggtgatgg tcgggtccga gttgacggtc tcggtggcaa ggcggccgtc
     1321 ggggcggtag gtgagctcgg cggtgtaggt ctcccatgtt gacgtggtgt tggggaggac
     1381 ttccgtgaag ctggcgagct ggccggcatc gttgtagtcc catgcccggg tcgctccgcc
     1441 tgctgcgtgg atgagctcgg aggtctggtg gccgtcgctg tcgtagctgt acgagacgta
     1501 ggggacggtg atcccgcccg atgtgacgga tacatcggtg agccgtccgg cggggtcgtg
     1561 gaggtaggtg ctgatggcgg cgccgtcggg gtagccgagg gaggtctgtt cgccagcgag
     1621 gccgtagctg tggctgatcg tgccctgcgg accggtttcg gtgactttct gccctgctcg
     1681 gtcgtaggac gaggtccacg tcttcgctgc tccggcgccg gtggcggagg tcgacttggt
     1741 gcgccagccg cggccgttga cccagaactt gttcacgacc gcggcgtcag tggagcgtgt
     1801 gaaggtcgtc tgcttgagtg tcccgtcgtt gtagtgggca tgcgtctgga cccggctggt
     1861 cgggtccgtt gcggtggcga gccagccgta gtcggggtcg taggtcgcca tgctcgtctg
     1921 cgatggtgcg ccggctcgcg ggggcgggac ggtgtgggtc agttgttggt tggcgaggtt
     1981 ccacgtccac gactcgatga cggggttcga tgcgtcgtcc gttgcggtgc gcgacgtccg
     2041 gttcccgcgg gagtcgtagc cgtaggtgac ggtgttgccg ttggcatcgg tgaccgtcgc
     2101 aacccgcccc gccgacgtgt acgtcgtggt ccgggcggac gggtcggtca cgccggtctg
     2161 ggcgatgtgc gggtcggtct ccgagatgac tcggcctgcg gcgtcgtagc cggtgagcgc
     2221 ggtgacagtg gtggggtcgc ccggtccttc gagacctgac ggggagacga ccccggtccg
     2281 gtggcctgcg gcgtcgtaca ggtagtggat ctcggcgccg cctggtgagg tcacggccga
     2341 gatgcggccg tcctcgtcat aggccgtctc ggtgatgccg ccgtctggag ccgtggtgtt
     2401 gacccggcgt ccggcgtcgt cgtagctgaa gctgacgacc gacttggcgg cgtcagggct
     2461 cgcccagtcg aacgtggcga catcgactgg ggggccgagg acctgggcgg tgcgcccgcc
     2521 gagggtgtaa cgggtctcgg tcacccggtc ttcggcgtgg atggtcgtgg ggttggcggt
     2581 gtcggtgtcg ttgtggcggt gctcgatcgt ccgccagagc cgcccggcgc tgtcgtacac
     2641 gtacccggtg aggagctgct cggctgctgg gccgccggag ccttcgagca tcgagaccgt
     2701 ccgcccggcg ctgtcgtagg tgtacgacac cttgcgctgc acggcggtgc cgtcggggtc
     2761 cgtgccgagg ggcagcgtct tcgtgaggac ctggccgcgg gcgtcgtact ccgttgagaa
     2821 cgtgtgcgcg gcgtcgtcgg ggtcgggacc gatcgtcgta ccggtggtcc ggccttcggc
     2881 gtcgtagtgg aacgaggtca cgatgcccgt cgggcccgtc gaggacacga gtcggccgag
     2941 gtcgccgtac gtgttcgtgg tcgtagccag ttcagccggg accgataggt ttccggtctg
     3001 ggagacgacg gtgtagccgg aacggtcgat cttgttgatc gacgagatcc catcggcgtc
     3061 ggtggagatg cgcacttcgg cgatgccggt cgcctctgac cagccgctgt cgccggggcg
     3121 atggacatcg aacgtggtgg tgtggtcggc cggatccgtg actgaggcga cggatccgtc
     3181 gagccggtag gccgtgcggg tcgccttgtg cgacgtgccg ttgtagagcg ggtcccgaga
     3241 ttctcgggtc tgcccttggg cgtcgcggcc ttcccatgac accgcgcccc cgggagactc
     3301 cgtgcgggag gcttcagcga tgagcgggtc gctgaagggc cacccggagt ccccgggatg
     3361 gagcgtgtcg aatgtcgtcg tccggggtgt gccgtcgtcc tcgtaggtcg tgctcgacgc
     3421 gagcgaccgg tccgcatggt acgtgtatga cgtcttcact ccgtcggggt cgacctcgtc
     3481 tttcgtcaac cccgccaggc tgccggtgaa gtagcggtag ctcgtggtcg ccttgccgct
     3541 cgacgggcac gacagccctg ccccgaggct cgctgcgtcg cacggcaccg acaccgtgtc
     3601 cggctcgttg gcgtcaccgt cgtaggtgta gttggtggtg accccgtcct cgcccgtgga
     3661 cgtcgctatg cggtcgtcgg ccctcgccgc ggccgacgag tccggcacca cgtaggtcag
     3721 cgtcgaggtc acacgtgtgg aaccgccagc cgtctccgac acggtgaggg gccgcccggc
     3781 ggcgtcgtag gtgtacgagg tggtggcacc ggagcgcgcc tcgaacgacg ttggcttgcc
     3841 ttcctcccac gacttcgaga tggcctgccc ggcagcgtcg gtgacaccca ggacctcgcc
     3901 gttcagtccg tactcgtaga ccgtggtcgc gcccgacgca gcgtcggtga cggtggtaaa
     3961 cccgggcgct gcgacgagac ccgctggtgg gttcggcttc aggtcgtact ggaaactcga
     4021 cacatcaccg gtatcgctga cctgctcggc cacgcggcca tcggtctcgt aggtgttctc
     4081 gaccagggtg tgctggcggg ttgcatcgac gtcgtcgacg atcgacgtga tccggagctg
     4141 ctcgtcgacg tcgtaggtgc gggtgccgaa cgtccccgcg gcctgaccga caaggtgcgg
     4201 cttcgacacg gtgtgcagca cggtccgctg ggacaccacc tggtagccgt agtcgacctg
     4261 ccggccctcc gagtccacca cacgatccac gaggccatcg gggcccgctg cgagcacgta
     4321 ctccgaggac tcctcgtcga ggacccagcc ctggtcgtcg gtcagctcga cgctgtaccc
     4381 gctccccgct gacacggtca cggatccaga gccttcgcct tcccaatcca gcgaaaccac
     4441 cggatccccg ggcctctcga ccgagatgag tcttccagcg gagtcgaagg tcgactccgc
     4501 accatcggtc tctgtgaggg tccagttcag cgcatcaggt tcgaggactg gcaggtatcc
     4561 ctcctcgttc gcccagctgc ccgatgttgc cgagtaatcg acgattcgac cgtctggcag
     4621 ctcgacagaa acgacgtcgg ccccatctgc aaccttgaac gccagacgca tgtcgaacgc
     4681 ggtcgcccaa cctggtccga agatgccgga ctgctcgccc agagacgagt agtccctgcg
     4741 gatcgctgtc accgatgtcc ctgacggaga cgagaggtcg ctctcgtgct gtgtaaagga
     4801 cccagtagcc acctccacgg gatcagcatg gtttgcgccg gtctccgggt cggggatctc
     4861 gctggagccg atcaggtaca ggccggagtt gttgccgtag cgggcgaggg gcgctcccga
     4921 ttgcattgcg accgtgaagc ggccgttgct cccgccgacg gcgagcgacc ctcccccgtt
     4981 cgagttgtcc tgcatctgcc tcgatgcgag cgatgctctg cggcggtccg tcgggctggc
     5041 cgacgccgcg aggtcagcat cgaagacacg cagcttcgag tccgtcaaga cgggaagttc
     5101 accgctggtg tcgtagctgc gggccatgat ggcgatgcga gtgccgtcag ccgagatcga
     5161 gggcactccc ggccggtcgc cgatgtccac cgccgtcagg gcggaactcg ggctgacgac
     5221 gtaccgggac gacgaggcgg tgctcgagtc gcgggtccac agggcgaggc gagacgacgc
     5281 accggagtct tggatgtagg ccacccggga cccgttctgg ctcatgctgc cgaacttggc
     5341 gctgaccggg tcgccgctgc tgtccttcga caccagctca aaggtccagt catcggcacc
     5401 gaccgcggag cgggtcgcca tgtagagcgc gtcgatcggc tcctccgggt cgatcaggcc
     5461 gggcaccact ccggtcgaag cggtcgcgaa cagcacgtgc aggccgtcgt cggagacctg
     5521 caccgacccg tagtcgtggt tgccgaaccg ctgccccata tccggctcca ggtccgcgtt
     5581 caggtacacc gggaccgggc cgcccccgtt gtcggagatg tagatcccgg cgtcgacggt
     5641 gaaccccgtg ctggcgcccc cgcacatgca gccgtcggca ctggcgcgga acgcgatcga
     5701 cgacccgtca gccgacaccg acggatgctg gctctggttc ccccagccag tacccagcgc
     5761 cgggaccttc cgaggcccgg tcgcgcccgt cgggaacggc tccacgtccc acggctgcgt
     5821 gagctccacc tcccagttct tggcgcacgg gtcgtcctcg atcgtctgct tcgtgagcag
     5881 ctcaggatca ccgaactcgc cgaagcgggt cgaccgccgc cagaccatga tctggccctc
     5941 gttgtagtac gagcggttca ccccgtcgca gtacggatca tcgaatctca ggtagacctg
     6001 ctgaccctga ctgttgttgg tctcggacca gtactcctgc cggctgaact tccccacgaa
     6061 ggtcctgcca tcggcgctca acacgccatc cgccgtgccc ggcgtaaaag aataagactt
     6121 cgagctgaag tggtagtagt cggtccacgc cagcacgccc tgtgtccacg gcagcgacag
     6181 caccttgagc ccgccgccgc cacctgaact cggcgagtcc agcacatagg ggagcccgct
     6241 cctattgggt gaaaaggcgg cggcactgcc agagaacgac accgagcgcc cgtccgaggt
     6301 catcgagacc tgcccattcc cggcgaacgc gttgcacttg cgcctcaaag gctcgcactc
     6361 ggcaacgccc tcgacggcgt caggaccgaa gcggagcacc tgccgcatcc caggagtcac
     6421 cgcgcccgcc ggcgccggcg ccgccatgac cccgacaacc acgctggcag ctgtggcgac
     6481 ggcgagcaga acgctcccag cggttctccc caaccggttc gaggacctgg cccgccgctc
     6541 gctcgcacgc cagcgcgcgg cccgattcac cagcaccatc acctgtcccc cacatcgatg
     6601 tcacagaccg agtagatctc ttcgaccggc gcagtcgcag cggcctccgc cttctccgga
     6661 ttcagaaccg cccctgctgc agcctcgacc tcagcagacg tcagcctccc gaactcccga
     6721 acagcgcacg ccagctgctt ctcggtgagc ccgccgccag ccgcccgctg gagatcctca
     6781 gcgaacctcg gcgcggcgcg cacctcgatc acacacgacg ccgccgcacc atcggccacc
     6841 gccagattcg ccgccgctcc cggtttctcg agcgcagcac ggaggtcctc gtcctcagtc
     6901 gcacgtcggg cgacgcagtc aagaacatcg tcgggatagg gcag
//
LOCUS       DATMFS010000321         3263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105550, whole genome shotgun sequence.
ACCESSION   DATMFS010000321 DATMFS010000000
VERSION     DATMFS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105550"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="VNQ33_05825"
     CDS             <1..2004
                     /locus_tag="VNQ33_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HWJ97659.1"
                     /translation="AAAYYSSTVENPMGLHSKTHFEIRDRQIVSAQREVYALDDADGG
                     AMTAIPDSKLADIEMRQGAGMRMSEIAGRIRKDQFEAISHPPDEPLLVVGAPGTGKTA
                     VGLHRLAYIMYGNADLRESPLVVGPNPGFLSFIKAVLPALAHTAEYSTAAGLHGGAAP
                     ARSEELDLERLKGDPRMATLIAAAVDNLKKVPSEPLVVRLPRSRVTLDVETLRSIFED
                     VDARGKPHNDKRADFRYELRRHIENVYRDRVGSHGPSRGTAISDLTTDVMRASDLMRL
                     VSSTWPSISAQALVRDLLGRARTLDQLGASVFRPDELELLYRAPAPRLEDEEWTFSDL
                     PLLDEAEALLSGVSAGASYVVLDEAQDLSPMELRSVARRIRKGAVTVMGDLPQATGEW
                     AYEAWEEVFEHLVPGQAEGFERVGLSIGYRVPQQILEIARRILAVAAPHEEVPEGVWY
                     GSDTPRRIECEPRNLVETVVQSVKDLASLELQRQSGGELTQPAGDVTDEEPDAELTAV
                     VTPSPLVGPVVEAFRSAGIRVARFDEDGIGDCVTVLRGIDSKGLEFDNVVIVEPSAVM
                     QERTLGHNVLYVAMTRAMKRLRVVHSEPLPPVLGDWTADPQRLADVESPTLDADRGVY
                     ASLVADDIQAYVASMLPQLPLDDLVAIYHLVRRLSGAPPRDLD"
     gene            2109..2246
                     /locus_tag="VNQ33_05830"
     CDS             2109..2246
                     /locus_tag="VNQ33_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97660.1"
                     /translation="MRDLEDCRLCQGRRFVLMLIRQGSRLTPSDLTPDDEDDRDLDDG
                     W"
     gene            2304..>3263
                     /gene="tig"
                     /locus_tag="VNQ33_05835"
     CDS             2304..>3263
                     /gene="tig"
                     /locus_tag="VNQ33_05835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWJ97661.1"
                     /translation="MKSTVEALEGNKVKLSIAVDESEFETDIDAAFKRIAREVRLPGF
                     RPGKAPRKVLEARIGLEAARGEAFEQALPKYYLEAVDEHDVDVIAQPEWEITAGKESG
                     DVAFEATVEVRPIVTVGGYDGLRVTIPSPHVDPTEIDARIDRLRESFAELQDVERPAQ
                     TGDNVTVDITGSRDGEAMDGLTAEDYLYEVGSGTIVPELDDQLAGAKVGAVLEFTADH
                     PLPDEEPVDFRVEVKNIKEKVLPEADDAFAAEASEFETIEELRADLEQRFSVVKKMQA
                     TMAVQQKTAEALSELVEEDAPDALVNQEMQNRLEDLGMRLQAQG"
BASE COUNT          571 a         1069 c         1090 g          533 t
ORIGIN      
        1 gccgctgcct actactcctc gaccgtcgag aacccgatgg ggctgcactc gaagactcac
       61 ttcgagatcc gcgatcgtca gatcgtgagc gctcaacgcg aggtctatgc gctcgacgat
      121 gccgatggcg gggcgatgac ggcaatcccg gattcgaagt tggcggacat cgaaatgcgg
      181 caaggcgccg ggatgcgtat gtccgagatt gcgggacgca tccgaaagga ccagttcgag
      241 gccatctctc atccgccgga cgagccgttg ctcgtcgtgg gtgctcctgg cacaggtaag
      301 actgccgtgg gcctccaccg gctcgcatac atcatgtacg gcaacgccga tctccgtgaa
      361 agcccgctgg tggtcggccc caatcctgga ttcctctcgt tcatcaaggc agttctccca
      421 gctctcgccc acacggcgga gtactccacc gcggctgggc tccatggcgg tgcggcaccc
      481 gcccgtagcg aggagctcga cctcgagcgg ctcaaaggtg acccccgcat ggctaccctg
      541 atcgccgcgg ccgtggacaa cctgaagaag gttccttcgg aaccgctggt tgtgcggctc
      601 ccgcgaagtc gtgtgaccct cgacgtggag acccttcggt ccatcttcga ggacgtcgac
      661 gcgaggggga agccccacaa cgacaagcga gcagacttcc ggtacgaact gcgtagacac
      721 atcgagaacg tgtatcggga tcgagtcggc agccacggcc catcgcgggg gactgcgatc
      781 tcagatctca ccaccgatgt catgcgcgcg tcggatctga tgcgcctcgt gtcgtcgact
      841 tggccttcga tctcagcgca ggcactggtg cgggatctac tcggtcgggc tcggacgctc
      901 gatcagctcg gagcgagcgt ctttcgcccc gatgagttgg aacttctcta ccgggcgccg
      961 gcaccgcggc ttgaggacga ggagtggacc ttctcggacc tgccgcttct ggatgaggcg
     1021 gaggcgttgt tgagcggtgt gtccgccggc gccagctacg tggtcctcga cgaggcgcaa
     1081 gatctctcgc cgatggagct ccgctcggtg gcgaggcgca tccgcaaggg tgccgtgacg
     1141 gtgatgggcg acctccccca ggccacgggc gaatgggcgt acgaggcctg ggaggaggtg
     1201 ttcgagcatc tcgtccccgg ccaagcggag ggcttcgagc gggtggggct ctcgatcgga
     1261 taccgagtcc ctcagcagat tctggagatc gcccggcgca tcctggcggt ggccgctcca
     1321 catgaggagg tgccagaagg ggtctggtac ggaagcgaca cgccgcggag gatcgaatgt
     1381 gaacctcgga acctcgtgga gaccgttgtc cagtcggtca aggacctcgc ttcgcttgaa
     1441 ctgcagcgtc agtctggagg cgagctgacc cagccggcag gtgacgtgac cgatgaagaa
     1501 cctgatgcag aactgaccgc ggtggtgacg ccgtccccgc tcgtaggccc cgtggtcgag
     1561 gccttccgca gcgccgggat ccgcgtcgcc cggttcgacg aagacggcat tggcgattgt
     1621 gtcacggtgc tgagaggaat cgactcgaag gggcttgagt tcgacaacgt cgtcatcgtc
     1681 gagccttcgg ccgtgatgca agaacgcact ttgggtcaca acgtcctcta cgtcgcgatg
     1741 acccgcgcca tgaagcggct ccgtgtcgtc cattccgagc cgcttccgcc cgtactgggc
     1801 gattggacag cagacccaca acgcttggca gatgttgaat cgccaacgct cgacgcggac
     1861 cgcggggtgt acgcctcgct tgttgccgac gacattcaag cgtacgtcgc gtccatgctt
     1921 cctcagctgc ctctggacga cctggtggcg atctaccacc tggttcgtcg actctcaggg
     1981 gctccacctc gagacctgga ttgaccgccc cgcatccacc cgcctgcccg tgggcgcgaa
     2041 gtcgcgggct cgtctctgtc gcggtccgct ggcggccgta ggaggcagcg cccgcccgcg
     2101 ccccgccggt gcgcgatctg gaggactgcc ggctgtgcca gggcaggcgg ttcgtgctga
     2161 tgctgatccg acaaggatcc cgcctgaccc cgagcgatct gacccctgac gatgaggacg
     2221 accgggatct cgacgacggc tggtgacggc gacgtccacg cattcggggg gtgtcggcgg
     2281 gtcccgatag gctctccacc ctcatgaagt caacggtcga agccctcgag ggcaacaagg
     2341 tcaagctgtc catcgcagtc gatgagtcgg agttcgaaac cgacatcgat gccgcgttca
     2401 agcgcatcgc acgggaggtc cggctgcccg ggttccgtcc cggcaaggca cctcgcaagg
     2461 tgctcgaggc ccgcatcggg ctcgaagccg cccggggcga ggccttcgag caggcgctgc
     2521 cgaagtacta cctcgaagcg gtcgacgagc acgacgtcga cgtcatcgcc cagccggagt
     2581 gggagatcac cgccggcaag gagagcggcg acgtggcctt cgaggccacc gtcgaggtgc
     2641 gcccgatcgt caccgtcggc ggctacgacg gcttgcgggt caccatcccg tcgccccacg
     2701 tggaccccac cgagatcgac gcccggatcg accgcctccg cgagagcttc gccgagctgc
     2761 aagacgtcga gcgcccggcg cagaccggcg acaacgtgac cgtcgacatc accgggtccc
     2821 gtgacggcga ggccatggac ggcctgaccg ccgaggacta cctgtacgag gtcggcagcg
     2881 gcaccatcgt gcccgagctc gacgaccagc tggccggcgc gaaggtcggc gccgtgctcg
     2941 agttcaccgc cgatcacccg ctgcccgacg aggagccggt cgacttccgc gtcgaggtca
     3001 agaacatcaa ggagaaggtc ctccccgagg cggatgatgc gttcgccgcc gaggcctccg
     3061 agttcgagac catcgaggag cttcgagccg acctggagca gcgcttctcc gtcgtcaaga
     3121 agatgcaggc caccatggcc gtccagcaga agacggccga ggcgctgtcc gaactggtgg
     3181 aggaggacgc gcccgacgcg ctcgtcaacc aggagatgca gaaccggctg gaagacctcg
     3241 gcatgcgcct ccaggcccag ggg
//
LOCUS       DATMFS010000322         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_81173, whole genome shotgun sequence.
ACCESSION   DATMFS010000322 DATMFS010000000
VERSION     DATMFS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81173"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VNQ33_05840"
     CDS             complement(<1..539)
                     /locus_tag="VNQ33_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ97662.1"
                     /translation="MSTNTRSSNEALIGRADVNDIEAILAVAAEEGEENVRAVKDNAD
                     AIFTWDYEKGRRPALNKLYEKAKHSQWNGETDLDWSIEVDPLELVEMQRHSFGQTPET
                     RAAQIAGTPFEKWGDKEFDQLGMESNNWMLSQFMHGEQGALICTAKIVETVPWIDAKY
                     YAATQVMDEARHVEVFAKYL"
     gene            complement(805..1725)
                     /locus_tag="VNQ33_05845"
     CDS             complement(805..1725)
                     /locus_tag="VNQ33_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ97663.1"
                     /translation="MPEPTGNAAFDAARAGGVTDTFLSFPDVREQKAKVYDYIRKASL
                     DKETQTMEMPAGYMFKDIPTYDEIDDPLELVLAEMERFGITRFLTNVTEKDLAKRALT
                     DHPDKFWGILNVDPNTGMEALRQIDAAVETWGDRLKSVHTWGTALLPQVPLDDKKMYP
                     IYAKCAEAGLPIVVYAGVPGPRLPMNAQLPMQLDEVCWFFPELKIVTRHGAEPWTELM
                     VKLLLKWPNLYYSTSAFAPKHYPSDIIDFANTRGSDKIIYAGYWAAGLTLDRIFAELP
                     DVPFRDEVWPKFLRENAERVFGGAPHPYPG"
     gene            complement(1776..1973)
                     /locus_tag="VNQ33_05850"
     CDS             complement(1776..1973)
                     /locus_tag="VNQ33_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97664.1"
                     /translation="MGRQAAGASSEVQNVAWVLVVLVILGGLMRGLGRFDTGIPDVVL
                     WGLIGVIVVAAVGRSVRSRRR"
     gene            complement(2076..>2336)
                     /locus_tag="VNQ33_05855"
     CDS             complement(2076..>2336)
                     /locus_tag="VNQ33_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97665.1"
                     /translation="PLFGSRASFHWGEIQVIGVRTVRAARGRGQALMIGASDDREVKV
                     DGLWVGLTGRNLHRIQDAVEQFAASTGVARPAFGFEPEPDPW"
BASE COUNT          373 a          810 c          761 g          392 t
ORIGIN      
        1 aggtacttgg cgaacacctc gacgtggcgg gcctcgtcca tcacctgggt ggcggcgtag
       61 tacttggcgt cgatccacgg gaccgtctcg acgatcttcg ccgtgcagat gagcgcaccc
      121 tgctcgccgt gcatgaactg gctgagcatc cagttgttcg actccatgcc gagttggtcg
      181 aactccttgt cgccccactt ctcgaacggc gtgccggcga tctgggcggc ccgggtctcg
      241 ggcgtctggc cgaaggagtg ccgctgcatc tccaccagct ccagcggatc gacctcgatc
      301 gaccagtcca ggtcggtctc gccgttccac tggctgtgct tggccttctc gtagagcttg
      361 ttcagcgccg ggcgccggcc cttctcgtag tcccacgtga agatggcgtc ggcgttgtcc
      421 ttgaccgccc ggacgttctc ctcaccctcc tcggccgcca cggcgaggat cgcctcgatg
      481 tcgttgacgt cggcgcggcc gatcagtgct tcgttggatg agcgcgtgtt ggtcgacatg
      541 acccggttcc cctcgttcgc ggtgcaagcg tcgccaaccc atcggcacga cgacgtcaat
      601 aacgaacgtt attgcaggtc gatgacccag ggaacaggcc atccgtcaca caactgttgc
      661 aagcacccgg ccccaccccg cgatttcggg tcacgcgtgc accccggagg tgcacagacg
      721 acccgaaatc accccacccg ccgcacccca tcgatttcag gtcacgcgtg cacccgggag
      781 gtgcacaggt gacccgaaag ctggtcagcc cggataggga tggggagcgc cgccgaagac
      841 gcgctcggcg ttctcgcgga ggaacttcgg ccagacctcg tcgcggaagg ggacgtcggg
      901 gagctcggcg aagatgcggt cgagcgtcag gcccgcggcc cagtagccgg cgtagatgat
      961 cttgtcgctg ccccgcgtgt tggcgaagtc gatgatgtcg gacgggtagt gcttcggggc
     1021 gaaggcggag gtcgagtagt agaggttcgg ccacttgagc agcagcttca ccatcagctc
     1081 ggtccacggc tcggcgccgt ggcgggtgac gatcttgagc tccgggaaga accagcagac
     1141 ctcgtcgagc tgcatgggca gctgggcgtt catgggcagg cggggcccgg ggacaccggc
     1201 gtagacgacg atcggcagcc cggcctcggc gcacttggcg tagatcgggt acatcttctt
     1261 gtcgtcgagc gggacctgcg ggagcagcgc cgtcccccac gtgtgcaccg acttgagccg
     1321 gtcgccccag gtctccaccg ccgcatcgat ctggcggagc gcctccatcc cggtgttggg
     1381 gtcgacgttc aagatccccc agaacttgtc gggatggtcg gtcagcgccc gcttggccag
     1441 gtccttctcg gtgacgttgg tgaggaagcg ggtgatgccg aagcgctcca tctcggccag
     1501 caccagctcg agcggatcgt cgatctcgtc gtaggtcggg atgtccttga acatgtaccc
     1561 agccggcatc tccatcgtct gcgtctcctt gtccagggac gccttgcgga tgtagtcgta
     1621 caccttcgcc ttctgctccc ggacgtcggg gaacgagagg aacgtgtcgg tgacgccccc
     1681 cgcacgggcg gcgtcgaagg cggcgttgcc ggtcggttcg ggcatgtccc ggaccctagc
     1741 gccggaacta gaacgcgttc tcgtttcctc gcagctcagc ggcgccggga tctcaccgac
     1801 cgacccaccg cagccaccac gatcaccccg atcaggcccc acagcacgac gtcagggata
     1861 ccggtgtcga agcggccgag accccgcatc agtcctccga ggatgaccag cacgaccagg
     1921 acccacgcga cgttctggac ctcgctcgac gcaccggccg cctgtcggcc caacgcagag
     1981 cggatcagcg cagcgacgtc gcccggcgac gagtcccccc tcgcccaccc ggcttgtcta
     2041 ctccaccgcc gcgccgagat cgagacccga gcggctcacc agggatccgg ctccggctcg
     2101 aacccgaacg ccggccgagc gacgccggtg gaggcggcga actgctccac ggcgtcttgg
     2161 atccggtgca ggttgcgacc ggtcaacccg acccacagcc cgtcgacctt gacctcgcgg
     2221 tcgtcgctgg cgccgatcat cagcgcctgc ccccggcccc gggcggcccg cacggtgcgg
     2281 acgccgatga cctggatctc gccccagtgg aacgaggcgc gggacccgaa caacgg
//
LOCUS       DATMFS010000323         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_220444, whole genome shotgun sequence.
ACCESSION   DATMFS010000323 DATMFS010000000
VERSION     DATMFS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220444"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VNQ33_05860"
     CDS             <1..809
                     /locus_tag="VNQ33_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ97666.1"
                     /translation="FERLELKADLVRAIDDGQLRCHYQPIVSLQTGRITGVEALVRWE
                     HPTRGLLYPDAFIPLAEDTGLIVPLGRWVMREACQQLRAWQLRLPASSTMSMSINLSV
                     RQLMHEQIIRDVREAVGDAGLDPSTVTMEITETTLMHDTEMTRERLAELREIGVSLAV
                     DDFGTGYSSLQYVQRFPIDIIKIDRSFVTGLGTNPGDGAVVQSMIELSQRLGVHTVAE
                     GIDRPEQVTLLQSLGADLGQGYLFSKPVEANRISALLDSSPHENPKFLLH"
     gene            966..2168
                     /locus_tag="VNQ33_05865"
     CDS             966..2168
                     /locus_tag="VNQ33_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ97667.1"
                     /translation="MSTTPAFVSRLVRLPLTDADGDPVGRIRDVVLAPSGDKLRVLGL
                     VANIERRNIFINMSRIAEIDPSGVRMVGGTVSVRSFTRRSGEKLAMGDLLDTKIDDEY
                     VTDLAIAPGPDKAFWYVVLAALGRRGPLRRRAPNVVPWEQVASSFASTPEMDEVAELR
                     DLHPTDVVDRLQNLSVARRHEVVALLADEQVADLLEEMEEEEAVRLVETLDLERAAHI
                     LEEMEPDDAADLLADLPETERAALLEEMRPEESIPLRRLLAYDEDTAGGLMTPEPVIL
                     AHTTTVAEALASIRMREIPAELAAQVFVTQPPVSTPTGKFVGVVGFQRLLREQPATTL
                     DECVNDSTEPVTPDLPLRDVAERLASYDLLALPVCDRAGRLVGAVTVDDVLDHVLPEG
                     WRRTRARA"
BASE COUNT          350 a          934 c          749 g          323 t
ORIGIN      
        1 tgttcgagcg cctcgagctc aaggccgacc tcgtccgcgc catcgacgac ggccagctcc
       61 gctgccacta ccagcccatc gtgtcgctgc agaccggccg catcaccggc gtcgaggccc
      121 tggtccgctg ggagcacccc acccgcggct tgctgtaccc cgacgccttc atcccgctcg
      181 ccgaagacac cgggctcatc gtgccgctcg gccggtgggt catgcgcgag gcgtgccagc
      241 agctccgggc ctggcagctg cgcctgcccg ccagctccac gatgtcgatg agcatcaacc
      301 tctcggtccg ccagctgatg cacgagcaga tcatccgcga cgtgcgcgag gccgtgggcg
      361 acgccggtct cgacccctcc accgtgacga tggagatcac cgagaccacc ctcatgcacg
      421 acaccgagat gacgcgcgag cgcctggccg agctccgcga gatcggcgtg tcgctcgccg
      481 tcgacgactt cggcaccggc tactcctcgc tccagtacgt gcagcgcttc ccgatcgaca
      541 tcatcaagat cgaccgcagc ttcgtgaccg gcctcggcac caaccccggc gacggcgccg
      601 tggtccagtc gatgatcgag ctgtcgcagc gcctcggcgt gcacaccgtg gccgagggca
      661 tcgaccgccc cgagcaggtc accctcctcc aatccctcgg tgccgacctc ggccagggct
      721 acctcttctc gaagcccgtc gaggccaacc gcatcagcgc cctcctcgac agcagccccc
      781 acgagaaccc caagttcctc ctgcactgac gccgtggacg ccaccgccgc ccgcggccgt
      841 cgccgggcga agcgcgcgtt cgtgcgccct ggagacgtcg ttgtgcacga agctcgaagc
      901 ggtgacgatc tgacgagcgg gtcgtcggct cggtcgaggg atgaccaggg gtagcgtccc
      961 acccgatgtc gaccactcct gccttcgtgt cgcggctcgt gcggctgccg ctcaccgatg
     1021 ccgacggcga tccggtgggc cgcatccgtg acgtcgtgct ggccccgtcg ggcgacaagc
     1081 tgcgcgtgct cgggctggtg gccaacatcg agcggcggaa catcttcatc aacatgagcc
     1141 gcatcgccga gatcgacccg agcggggtcc gcatggtcgg cggcaccgtc agcgtgcgct
     1201 cgttcacccg ccgctccggc gagaagctcg ccatgggcga cctcctcgac accaagatcg
     1261 acgacgagta cgtcaccgac ctcgccatcg cccccggacc cgacaaggcg ttctggtacg
     1321 tggtgctggc cgccctcggc cggcgaggcc cgctccggcg gcgggccccc aacgtggtgc
     1381 cgtgggagca ggtggcgtcg agcttcgcca gcacgccgga gatggacgag gtcgccgagc
     1441 tgcgcgacct ccaccccacc gacgtcgtcg accgcctcca gaacctgtcc gtcgcccgtc
     1501 gccacgaggt cgtcgcgctc ctcgccgacg aacaggtcgc tgacctcctc gaggagatgg
     1561 aggaggagga ggcggtccgc ctggtcgaga ccctcgacct cgagcgggcc gcccacatcc
     1621 tcgaggagat ggagcccgac gacgccgccg acctgctcgc cgatctcccc gagaccgagc
     1681 gggccgcgct cctcgaggag atgcggcccg aggagtccat cccgctccgc cggctgctcg
     1741 cctacgacga ggacacggcc ggcggcctca tgacccccga gccggtcatc ctcgcccaca
     1801 ccaccaccgt cgccgaggcg ctcgccagca tccgcatgcg cgagatcccc gccgagctcg
     1861 ccgcccaggt cttcgtcacg cagccaccgg tgtcgacgcc caccggcaag ttcgtcgggg
     1921 tggtcgggtt ccagcgcctc ctgcgcgagc agccggcgac cacgctcgac gagtgcgtga
     1981 acgacagcac cgagcccgtc acgcccgatc tgccgctgcg cgacgtcgcc gagcgcctcg
     2041 cctcctacga cctcctcgcc ctcccggtgt gcgaccgagc cgggcgcctc gtcggcgccg
     2101 tcacggtcga cgacgtgctc gaccacgtcc tccccgaggg ctggcgccgc acccgagccc
     2161 gcgcctgacg ctccgcgctt cgaaccgcgg tcgcgtgcgg gggaagcaga caccgatccc
     2221 taggatcgcc gcgtgagacg gacccttgct gccctcgtcg cctcggcgac gctgctcgcc
     2281 gcgtgctcca cggcgggcgg caacgagtcg gccgaggagc cgacgaccac cacctcgacc
     2341 accgccagcg acggcg
//
LOCUS       DATMFS010000324         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_57170, whole genome shotgun sequence.
ACCESSION   DATMFS010000324 DATMFS010000000
VERSION     DATMFS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57170"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VNQ33_05870"
     CDS             <1..1461
                     /locus_tag="VNQ33_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97668.1"
                     /translation="VVGLARMLLVALGASALALLLNAPWALDLVGGPAPWASIAGITS
                     TSADPLTLGEILRFETGPWGAPPLGWAFLLAGALPVIIGRSWRLEWAVRAWMVVLGGW
                     TVLWASQQGHLPGGLPSSEVILAPVAAALGLAAALGLAAFETDLRAYRFGWRQVLAVA
                     AAVGVVLGAAPLASGLIDGRWRTPTSSYAAGTSDLLASDGDGAFRVVWLGDPGVLPVS
                     GWPYDDHLAYGTSDQGPPTMVDRFVVPESGATPLIGDALHAAADRRTSRIGRLLGPMG
                     VRYLVVQSQLAPTDEAATGEATVAPALAVLDEQLDLERVPVADGLTVYRNLAWVPSRA
                     VLPPREGDRTSFTDAARDDLAEAQAALEQPDGVDGATGSVPGTGDLLIASTADGGWDV
                     RVEGVPLRRSTTYGWANQFEATRTGDGALRFDTPVAHRAVAVGEAVLWLLVLWAWRRA
                     RRRSRDAEAAAAAAAPRRRRAARDMGRLAEPEGVDA"
     gene            1461..>2001
                     /locus_tag="VNQ33_05875"
     CDS             1461..>2001
                     /locus_tag="VNQ33_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97669.1"
                     /translation="MAARRAARHRRSRRLTPRRWLPFAALLAVIVGAVMVSSEEPSRP
                     PASADPVLDTSRLPAVSASDAISTTWFCAGGTATGEGGIAEMTLVLANDAPRGAVAEV
                     TVMGEAGKEASEQVDVPAHGRARVAASELLEAEWIGAVVEVQGGRVAVDREVAGPEGY
                     DSAPCSPAASDHWFVPSGAT"
BASE COUNT          202 a          773 c          746 g          280 t
ORIGIN      
        1 gtcgtcggcc tcgcccggat gctcttggtg gcgctcgggg cgtcggccct cgccctcctc
       61 ctcaacgccc cgtgggcgct cgacctcgtc gggggtccgg cgccgtgggc gtccatcgcc
      121 ggcatcacca gcaccagcgc cgacccgctc acgctcgggg agatcctccg gttcgagacc
      181 ggtccgtggg gcgcgccgcc gctcgggtgg gcgttcctgc tcgctggtgc cctcccggtg
      241 atcatcggtc gctcgtggcg cctcgagtgg gcggtgcggg cgtggatggt cgtgctcggc
      301 ggctggaccg tcctctgggc cagccagcag ggccacctgc cgggcggcct tcccagctcg
      361 gaggtgatcc tcgcccccgt cgccgccgcc ctcgggctgg ccgcggccct cggcctggct
      421 gccttcgaga ccgacctgcg ggcgtaccgg ttcggctggc gccaggtgct cgccgtcgcc
      481 gccgccgtcg gggtggtcct cggggctgca ccgctggcca gcggcctcat cgacggccgc
      541 tggcgcacgc ccacctcgag ctatgcggcc ggcacgagcg acctgctcgc cagcgacgga
      601 gacggcgcgt tccgcgtggt ctggctgggc gatccggggg tgctccccgt gtcgggctgg
      661 ccgtacgacg accacctggc ctacggcacg tccgatcagg gcccgcccac gatggtcgac
      721 cgcttcgtcg tgccggagtc gggcgccacc ccgctcatcg gcgatgcgct gcacgcggct
      781 gccgaccggc ggaccagccg gatcgggcgg ctcctcggcc ccatgggggt gcggtacctc
      841 gtggtccaga gccagctcgc tcccaccgac gaggccgcga ccggcgaggc gacggtggca
      901 ccggcgctcg ccgtgctcga cgagcagctc gacctcgagc gcgtcccggt ggccgatggg
      961 ctcaccgtgt accggaacct ggcctgggtg ccgtctcgag ccgtgctgcc cccgcgggag
     1021 ggcgaccgga cctccttcac cgatgcggcg cgcgacgatc tggccgaggc gcaagcggcg
     1081 ctggagcagc ccgacggcgt cgacggggcc accggctcgg tgcccggcac cggtgacctg
     1141 ctgatcgcga gcaccgccga cggcggctgg gatgtgcgcg tcgagggcgt cccgctccgc
     1201 cgctccacca cctacggctg ggccaaccag ttcgaggcga cgcggaccgg cgacggcgcc
     1261 ctgcgcttcg acaccccggt cgcccaccgc gccgtcgccg tgggcgaggc ggtcctgtgg
     1321 ctcctcgtct tgtgggcctg gcgccgggcg cgccggcgca gccgcgacgc cgaagcggcc
     1381 gcggccgccg ccgcaccgcg ccgtcgccgc gcggcacgcg acatgggccg cctcgccgaa
     1441 cccgaagggg tcgatgcctg atggccgctc gccgtgctgc ccgccatcgt cgctcccgcc
     1501 gcctgacccc gaggcggtgg ctgccgttcg ccgccctcct cgccgtcatc gtcggggcgg
     1561 tcatggtctc ctctgaggag ccctcgcggc ctccggccag cgccgatccg gtgctcgaca
     1621 cgtcgcgcct cccggccgtc agcgccagcg atgcgatcag caccacctgg ttctgcgccg
     1681 gcggcaccgc caccggcgag gggggcatcg ccgagatgac gctcgtgctc gccaacgatg
     1741 caccccgtgg cgccgtcgct gaggtgaccg tcatgggtga ggccggcaag gaggcgagcg
     1801 agcaggtcga cgtgcccgcc cacggtcggg ctcgtgtcgc cgcgtcggag ctcctcgagg
     1861 ccgagtggat cggtgcggtg gtcgaggtgc aggggggacg ggtcgccgtc gaccgcgagg
     1921 tcgccgggcc cgaggggtac gactcggcac cgtgctcgcc ggcggccagc gatcactggt
     1981 tcgtgccctc cggtgcgacc a
//
LOCUS       DATMFS010000325         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_242576, whole genome shotgun sequence.
ACCESSION   DATMFS010000325 DATMFS010000000
VERSION     DATMFS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242576"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..793
                     /locus_tag="VNQ33_05880"
     CDS             218..793
                     /locus_tag="VNQ33_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97670.1"
                     /translation="MSTNAADELERLWYDCRNMTGGRRTRWFTLPFSEAFAIIAFYRF
                     NRAAYLTFGRTWVLFRTLTSPLNPLIRIFVPTQIDYRADIGPGFRILHPQLGVVIGGR
                     VRAGKGLILAGGNCIGGGAAVLGNWVQLGANATVMGEVTLGNGVRIGAGAVVVKDFAG
                     PGTLVGVPAEPVGTPTGDATATTLDEADFTI"
     gene            820..>2321
                     /locus_tag="VNQ33_05885"
     CDS             820..>2321
                     /locus_tag="VNQ33_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWJ97671.1"
                     /translation="MADDLPWWASTVIYQVYPRSFADSNGDGIGDLPGITAHLDHLVD
                     LGVETLWVSPFFASPQHDVGYDVSDYRDVAPEYGTLDDAQELIDTAHGKGLKVMFDLV
                     LNHTSEQHPWFIESRSSRDNPKADWYVWADGYRSRSGKPKPPNNWRSELQIPRAWQWG
                     EERQQWYLATFLWFQPDLNWRNPELRAEMFDMVRMWLGRGVDGFRLDIFGSIMHDEAL
                     RDNDPKPTLMNGMPRLQTPSRTVNTDENFGVAVDLREVCDEFAGDDRVLIGEVFGPPS
                     ILRRYIEDQGRPGLHLVFLFEFLSAPYSARRYNQLIERFEREFPAPLVPTYVVENHDR
                     ARSLSRVGGDLARARVLATLLCTLRGVPTLYMGQEIGMENTYIPLAEANDPIPSVVAK
                     RLPEWLNKKLPERLNRDEVRTPMQWTGEPGAGFTEPGVATWLPIHENHRTVNVADERD
                     DPGSLLNWYRALLALRRNLPVLQTGALDLAADAPDDVIRFERTLDHTTVSV"
BASE COUNT          353 a          847 c          767 g          354 t
ORIGIN      
        1 acccgcggca aggcgccctg gcacttctgg gtgctggtcg ccgccctcgt cgcgtacctc
       61 ggctggcgcc tcgtgcagac cgtcatctgg gccgtcaccg gccactggcc gggctgagcg
      121 cgtgccgcgg cctgcgagac cgatcctcta gtctcgcccc gggtctcggt cgacagcgtt
      181 ggggagagcg atggttgggt cggcggtggt tccaccggtg agcacgaacg cagcagacga
      241 gctcgagcgg ctctggtacg actgccgcaa catgaccggt gggaggcgga cccgctggtt
      301 caccctgccg ttcagcgagg ccttcgcgat catcgccttc taccggttca accgggcggc
      361 gtacctgacc ttcggtcgga cgtgggtcct gttccggacc ctgacatcgc cgctcaaccc
      421 gctcatccgg atcttcgtcc ccacccagat cgactaccgg gcggacatcg gccccgggtt
      481 ccggatcctc catccccagc tcggcgtcgt gatcgggggc cgcgtccggg ctggcaaggg
      541 gctgatcctc gccggaggga actgcatcgg cggcggtgcg gccgtgctcg ggaactgggt
      601 ccagctcgga gcgaacgcca ccgtgatggg ggaggtgacc ctgggcaacg gggtccgcat
      661 cggcgccggc gccgtggtgg tgaaggactt cgccggtccc gggaccctgg tcggcgtgcc
      721 agccgagccg gtcggcaccc ccaccggtga cgcgaccgcg accaccctcg acgaggccga
      781 cttcaccatc tgacgtccct cggctcctac gatccggcga tggctgacga tcttccctgg
      841 tgggcgtcca ccgtcatcta ccaggtgtac ccgcggtcgt tcgcggactc gaacggggac
      901 gggatcggcg acctgccggg gatcaccgcc cacctcgacc acctggtcga cctgggggtc
      961 gagacgctgt gggtctcgcc gttcttcgcc agtccgcagc acgacgtcgg ctacgacgtg
     1021 tcggactacc gcgacgtcgc ccccgagtac ggcaccctcg acgacgccca ggagctgatc
     1081 gacaccgccc acggcaaggg cctcaaggtc atgttcgacc tggtgctcaa ccacacgtcc
     1141 gagcagcacc cctggttcat cgagtcccgg agctcgcgcg acaacccgaa ggccgactgg
     1201 tacgtgtggg ccgacggcta ccggagccgg agcggcaagc cgaagccgcc gaacaactgg
     1261 cgctcggagc tccagatccc gcgggcctgg cagtggggcg aggagcgcca gcagtggtac
     1321 ctggccacgt tcctgtggtt ccaacctgac ctgaactggc ggaaccccga gctgcgcgcc
     1381 gagatgttcg acatggtccg catgtggctg ggacggggcg tcgacggctt ccgcctggac
     1441 atcttcgggt cgatcatgca cgacgaggcc ctgcgcgaca acgatccgaa gccgaccctc
     1501 atgaacggga tgccccggct ccagaccccg tcgcgcacgg tgaacaccga cgagaacttc
     1561 ggcgtagccg tggacctccg cgaggtctgc gacgagttcg cgggcgacga ccgcgtcctg
     1621 atcggcgagg tgttcgggcc accatcgatc ctgcgccgct acatcgagga ccagggccgc
     1681 cccggcctgc acctcgtgtt cctgttcgag ttcctgtcag cgccgtactc cgccaggcgc
     1741 tacaaccagc tgatcgagcg gttcgagcgc gagttcccgg caccgctcgt gcccacgtac
     1801 gtcgtcgaga accacgaccg ggcccggtcg ctgtcgcggg tgggcggcga cctcgcccgt
     1861 gctcgcgtcc tcgccacgct gctctgcacc ctccgaggcg tcccgaccct gtacatgggc
     1921 caggagatcg ggatggagaa cacctacatc ccgctcgccg aggccaacga ccccatcccg
     1981 tcggtggtgg ccaagcgcct ccccgagtgg ctgaacaaga agctgcccga gcgcctcaac
     2041 cgcgacgagg tgcggacgcc gatgcagtgg acgggggagc cgggcgccgg cttcaccgag
     2101 cccggcgtgg ccacgtggct gccgatccac gagaaccacc ggactgtcaa cgtcgccgac
     2161 gaacgagacg atcccggctc gctcctgaac tggtaccggg cgctcctcgc gctccgcagg
     2221 aacctgcccg tgctccagac cggcgccctc gacctggcgg ccgacgctcc cgacgacgtc
     2281 atccgcttcg agcgcacgct cgaccacacc accgtctccg t
//
LOCUS       DATMFS010000326         7433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_172487, whole genome shotgun sequence.
ACCESSION   DATMFS010000326 DATMFS010000000
VERSION     DATMFS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172487"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VNQ33_05890"
     CDS             <1..532
                     /locus_tag="VNQ33_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ97672.1"
                     /translation="FEETFLFSDTIAANIAFADASASIDDIERAARLAGAHQFITGLS
                     HGYDTEIGERGFSLSGGQRQRIAIARAILADPRVLILDDATSAVDPTKEHEIRDALGE
                     VMRDRTTVVIAHRPATIALAERVVLIGDGGVVADGTHEGLLRTSEAYREVLASAAADE
                     EQARHAALEGEGVVAP"
     gene            532..2316
                     /locus_tag="VNQ33_05895"
     CDS             532..2316
                     /locus_tag="VNQ33_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97673.1"
                     /translation="MPMSAIDEDSKLDRRQAAQVLRRAFRMLKPYKRDVAGAGFLVTV
                     STLAVLAGPFLLKVGIDKGISEGDEATLDLAVAAFVVVAAVAYVASRIQVLMISRAGE
                     GFLRDLRNRVFDHLLKLSMPFYDREKAGVVISRMTSDVDSLQELIQQGLLQLLASVLL
                     IVGSVVFLGIVSWQLLLVCLIPVPFVVLASIKFQRDSNEAYLTVRDRIGLTLSSLQEG
                     ISGVRVIQAYGREDVEIGRFESRNNSLYRAHMKSVFVQAWYLPVIEGAAVGCTALVVA
                     IGGRMAVSGAASVGTVAFFVLTLSNLFEPLQQLSQLFNTVQSSGAALQKLFDLLDTPV
                     DLEERAGAVALADHGELRVEGLSFAYGSGPPVLSAVDLVLPEGEKLALVGPTGAGKST
                     LAKLMARLYDPTEGRVTFGGTDLRDAQLTSLRRKITVVPQEGYLFHGSIRDNVRIGRP
                     DATDQEVSDALESLGLLARFASQAEGLDTEVNERGSSLSAGERQLVSLARAALADPAV
                     LVLDEATSSLDPGTEVLVETALERLVRGRTVIVIAHRLTTAERSDRVGVVADGRLVEL
                     GTHADLVAQGGHYARLYRSWVHGLAASG"
     gene            complement(2442..3833)
                     /locus_tag="VNQ33_05900"
     CDS             complement(2442..3833)
                     /locus_tag="VNQ33_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97674.1"
                     /translation="MNTLLRTVLRTVLLAAGLWLVSVTAPALTSAASAAPTTPAEGPQ
                     LIDLTVDIPVGLCGTTIALIGDTHTTCPAPPPPPPPAPENPALIDVDLTIPVDLCGVT
                     VGIIADTATTCGTPAPTPAPAPAPEPAGGLVDIDATIPVDLCGVTVGVIADTAAACSS
                     PAPAPAPAPSTPTSPGGALVAIPVSAPVDVCGITVGVIAGTDTTCSPAPAPVPTSPSG
                     GVVSVPVTAPVSVCGIAVGVIADTGTTCTGPTTPTAPTTPSTPANPGGGLIDLPVDIP
                     VDLCGITVGLIADTGTTCTSGPSDPGGPGIDIPVDLCGITVGLISDTDTTCNPGGPDD
                     PGIDLCGVTVGILGSSDTTCGNVDPTDPAGPVDPTNPVDPTNPVDPVDPTKPVDPANP
                     AHPTGPEGPSVLDESTPDPATGDPHGASAAPAVGTGTLARTGAPLGELTELGLLLLAA
                     GLVLLRGRRRIVT"
     gene            complement(3964..5046)
                     /locus_tag="VNQ33_05905"
     CDS             complement(3964..5046)
                     /locus_tag="VNQ33_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97675.1"
                     /translation="MGRRPQNVIIHDHRQDTMAARRIAAEETGDRSTAGARSAVLRLL
                     TAALAGGAVLLLWGGAAGARAADPAPSAPAPSATNDGSLLNGLLGEGGALTPVIDLVD
                     RVTQPLTGSTPVGATVDGVVATVDPIVSPVTAPVTEAAGPVVAPVVDAVGDLAEGVTT
                     ALTNPAPGTPTTPTTIVPPAVPPVAGPGSLPLPELPPLLPPSASTEPGTTSGTAPAGG
                     AAPAAAHAAASLPLALAAGPEGAADALTGSAAGASDRSDDPPAAVTGAAAQAARSALA
                     THDGSHDAAPGPHHGHGPAAPVAPAPAGCAGGSLSGPGPSPATGPAWLAADGLHGTAG
                     AARTIDAPSETTPARAPSAGPEVAPD"
     gene            complement(5125..6465)
                     /locus_tag="VNQ33_05910"
     CDS             complement(5125..6465)
                     /locus_tag="VNQ33_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ97676.1"
                     /translation="MSLADAIETRNAQGIAAGIGRLITAGDLPVGDRLPTVRALAREL
                     DVSPTTVSEAWRILSAVGAIEARGRNGTFVRQVPAPGAGHRYRRVTEGPGHFALDLST
                     GTPDPALLPDLGPIVALVARQNLTSSYLDPPVLPQLEESLRSVWPFQAESLTVVDGAM
                     DAIDRVAQVIVRLGDRVVVENPTFPPVLDLLDQLGAEVIGVDLDDEGISLTSLEAALA
                     QKPSMVFLQPRAQNPTGITTSGRRARAIAKLLKGTGALVVEDDHIGDISEAPPISVGT
                     WLPSQTVHIRSFSKSHGPDLRLAAVGGAGEVVDALATRRFLGPGWSSRILQAVLLALL
                     RDGRTIDQITRAQVEYAARRAAFAAALADHGIAAPGTDGINQWVPVADERSTTVALAA
                     QGIGVAPGAPFLVRPGTGHVRITIGLVDDPATIATLADQLAAADRAAARPSGRR"
     gene            6604..>7433
                     /locus_tag="VNQ33_05915"
     CDS             6604..>7433
                     /locus_tag="VNQ33_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ97677.1"
                     /translation="MKRISHWIDGRVVAGTSGREGVVWNPATGEQQAAVDLASAAELD
                     AAVAAAKAAFPAWRATGLSRRAEVMFRLRDLIDANREEIASLVTAEHGKVLSDALGEV
                     ARGLENVEFATGVPHLLKGGFTEQAATGVDVYSIRQPLGVVAGITPFNFPAMVPMWMF
                     ANALACGNTFILKPSEKDPSTALYLAELLARAGLPAGVFNVVNGDKEAVDAILANPDV
                     AAVSFVGSTPIARTIYETGTRAGKRVQALGGAKNHMLVLPDADLDMAADAAVSAAYGS
                     A"
BASE COUNT          937 a         2531 c         2917 g         1048 t
ORIGIN      
        1 gttcgaggag acgttcctct tctcggacac catcgccgcc aacatcgcgt tcgccgatgc
       61 cagcgcctcg atcgacgaca tcgagcgggc ggccaggctc gccggggcgc accagttcat
      121 caccgggctc tcgcacggct acgacaccga gatcggcgag cgcggcttct ccctgtcggg
      181 gggccagcgc cagcgcatcg ccatcgcccg ggcgatcctg gccgacccgc gcgtgctgat
      241 cctcgacgac gccacctccg cggtcgaccc caccaaggag cacgagatcc gcgacgcgct
      301 cggcgaggtc atgcgcgacc gcaccacggt cgtgatcgcc caccgaccgg ccaccatcgc
      361 cctcgccgag cgggtcgtgc tgatcggcga cggcggcgtc gtggccgacg gcacccacga
      421 gggcctgctg cgcaccagcg aggcctaccg cgaggtgctc gcctcggccg ccgccgacga
      481 ggagcaggcc cggcacgccg cgctcgaggg cgagggggtg gtggcgccgt gatgccgatg
      541 tccgccatcg acgaggactc gaagctggac cggcgccagg ccgcccaggt cctccgccgg
      601 gcgttccgga tgctgaagcc ctacaagcgc gacgtcgccg gcgcgggctt cctcgtcacc
      661 gtctccaccc tcgcggtgct cgccgggccg ttcctgctca aggtcggcat cgacaagggc
      721 atctccgagg gcgacgaggc gaccctcgac ctggccgtcg ccgcgttcgt ggtggtcgcc
      781 gccgtcgcct acgtcgccag ccgcatccag gtcctgatga tcagccgcgc cggcgagggc
      841 ttcctgcgcg acctgcgcaa ccgggtgttc gaccacctgc tgaagctctc catgccgttc
      901 tacgaccggg agaaggcggg ggtcgtcatc agccgcatga cgtccgatgt ggactcgctg
      961 caggagctca tccagcaggg cctcctccag ctactggcca gcgtcttgct catcgtcggc
     1021 tcggtggtct tcctcggcat cgtgtcgtgg cagctcctgc tggtctgcct gatccccgtg
     1081 ccgttcgtgg tgctggcgtc gatcaagttc cagcgcgact cgaacgaggc gtacctcacc
     1141 gtccgcgacc gcatcgggct cacgctgtcg tcgctccaag agggcatcag cggcgtccgg
     1201 gtgatccagg cctacgggcg cgaggacgtc gagatcggcc gcttcgagtc ccgcaacaac
     1261 agcctctacc gggctcacat gaagtcggtg ttcgtccagg cctggtacct cccggtcatc
     1321 gagggtgcgg cggtggggtg cacggccctg gtggtcgcca tcggcgggag gatggccgtg
     1381 agcggcgcgg cctccgtcgg cacggtcgcc ttcttcgtgc tcaccttgtc gaacctcttc
     1441 gagccgctcc agcagctgtc gcagctgttc aacaccgtcc agtcctcggg cgcggccctg
     1501 cagaagctgt tcgatctgct cgacaccccg gtcgacctcg aggagcgtgc gggcgccgtg
     1561 gccctggccg atcacggcga gctccgcgtc gagggcctgt cgttcgccta cggcagcggg
     1621 ccgccggtgc tctccgccgt cgacctggtc ctgcccgagg gcgagaagct ggccctcgtg
     1681 gggcccacgg gcgccggcaa gtcgacgctc gccaagttga tggcccggct ctacgacccc
     1741 accgaggggc gggtcacctt cggcggcacc gacctccggg acgcgcagct gacgtcgctg
     1801 cgccggaaga tcaccgtcgt gccgcaggag ggctacctct tccacggctc catccgcgac
     1861 aacgtgcgca tcgggcggcc cgacgccacc gaccaggagg tctccgacgc cctcgaatcg
     1921 ctcggcctgc tggcccggtt cgcctcgcag gccgagggtc tcgacaccga ggtcaacgaa
     1981 cgggggtcgt cgctgtcggc gggggagcgc cagctggtct cgctggctcg ggccgcgctc
     2041 gccgatccgg ccgtcctggt cctcgacgag gcgacgtcgt cgctcgaccc gggcaccgag
     2101 gtgctggtgg agacggcgct cgaacgcctg gtccgcggcc ggaccgtcat cgtgatcgcc
     2161 caccggctga ccacggccga gcggtccgat cgagtcggcg tggtcgccga cggccgcctc
     2221 gtcgagctcg ggacccatgc cgacctcgtg gcccagggcg gccactacgc ccgcctctac
     2281 cggagctggg tgcacggcct cgctgcttct ggctgatccg ccggtctcct cgtcctcgtc
     2341 ggcacgctgc acgcgccgcg gcgcgtgctg gggaggcatc gctgcctccc cagcaccatc
     2401 gagccgtgag gcccccaggg gtgggggcta gggaaggagc ctcatgtcac gatgcgccgc
     2461 cggcccctga ggaggacgag gccggctgcg aggagcagca gtccgagttc ggtgagctcc
     2521 cccagcgggg cgccggtgcg ggcgagggtg ccggtgccga ccgccggggc ggccgatgcc
     2581 ccgtgcgggt cgccggtggc ggggtccggt gtggactcgt cgagcaccga cgggccctcg
     2641 gggccggtcg ggtgggcagg gttggcgggg tccaccggct tggtggggtc gacggggtcc
     2701 accgggttgg tggggtccac cgggttggtg gggtcgacgg ggccggccgg gtccgtgggg
     2761 tcgacgttcc cgcaggtcgt gtcggaggat ccgaggatcc cgacggtgac gccgcagagg
     2821 tcgatgccgg gatcatccgg gccacccggg ttgcaggtgg tgtcggtgtc ggagatgagg
     2881 ccgacggtga tgccgcagag gtcgacgggg atgtcgatgc cgggtcctcc tgggtcgctc
     2941 ggtccgctgg tgcaggtggt gccggtgtcg gcgatgaggc cgacggtgat gccgcagagg
     3001 tcgacgggga tgtcgaccgg gaggtcgatg agcccgccac cggggttggc cggggtgctc
     3061 ggcgtcgtcg gggccgtggg cgtcgtcggg ccggtgcagg tggtgccggt gtcggcgatg
     3121 accccgacgg cgatgccgca cacgctgacc ggagcggtga ccgggacgct gaccacgccg
     3181 ccgctcgggc tggtcggaac cggcgccggt gcggggctgc aggtggtgtc cgtgccggcg
     3241 atgacgccga cggtgatccc gcagacgtcg acgggtgcgc tgaccgggat ggcgacgagc
     3301 gcgccgccgg gggaggtggg cgtcgacggt gccggtgccg gtgctggtgc cgggctggag
     3361 cacgcggcgg cggtgtcggc gatcacgccc accgtgacgc cgcacaggtc gaccgggatg
     3421 gtggcgtcga tgtccacgag ccctccggcg ggctcaggag ccggtgccgg agccggcgtg
     3481 ggtgccgggg tgccgcaggt ggtggcggtg tcggcgatga tcccgacggt gacgccgcag
     3541 aggtcgacgg ggatggtgag gtcgacgtcg atgagcgccg ggttctccgg tgcgggaggc
     3601 ggcggtggcg ggggtgcggg acacgtggtg tgggtgtcgc cgatgagggc gatggtggtg
     3661 ccgcagaggc cgacggggat gtcgacggtg aggtcgatca gctgtggtcc ttcggcaggt
     3721 gtggtggggg ccgcggatgc ggcggaggtg agcgcggggg cggtcacgct gacgagccag
     3781 aggcccgcag cgagcagcac cgtgcgcagc accgtgcgaa gcagggtgtt catggttcct
     3841 cctcaggaat cgggttggga ccgcgccggc gggggtgccg gcgtcggtgc ttgctgttcc
     3901 gtctggacgt ccacgcgccg ccgacccggg aggggtgggg gtgccggtcg tccgggagga
     3961 ggttcagtcc ggagcgacct cggggccggc gctgggggcg cgggcagggg tcgtctccga
     4021 tggggcgtcg atcgtgcggg ccgcgccggc ggtgccgtgg agaccgtcgg cggcgagcca
     4081 ggccggcccg gtcgccgggc tcggtcctgg gccgctcagc gacccgccgg cgcacccggc
     4141 cggagccgga gcgaccggcg cagccggacc gtgcccgtgg tgcgggccag gagcggcgtc
     4201 gtggctgccg tcgtgggtgg cgagcgccga ccgagcggcc tgagccgcag caccggtgac
     4261 cgctgcgggc gggtcgtccg agcgatccga ggcgccggcg gccgacccgg tgagggcgtc
     4321 cgccgcaccc tccgggccgg ccgccagggc aagggggagc gatgcggccg cgtgcgcggc
     4381 ggcgggagcc gcgccgcccg ccggagcggt accggaggtc gtgccgggct cggtcgaggc
     4441 cgacggcggc agcagcggcg ggagctcggg aagcggcagg gagccgggac cggcgaccgg
     4501 aggaacggcc ggggggacga tcgtcgtcgg ggtggtcggc gtgccgggcg ccgggttcgt
     4561 caaggcggtg gtgacgccct cggccaggtc gccgaccgca tcgaccaccg gtgccacgac
     4621 cgggccggcg gcctcggtca ccggagcggt caccggcgac acgatcgggt cgaccgtggc
     4681 cacgacgccg tcgacggtgg caccgaccgg cgtcgaaccg gtgaggggct gggtcacccg
     4741 gtcgacgagg tcgatcaccg gcgtcagcgc gccgccttcg ccgaggaggc cgttgagcag
     4801 gctgccgtcg ttcgtggccg acggagccgg ggccgacgga gccgggtcgg ctgcacgggc
     4861 cccggcggct ccgccccaca ggaggaggac ggcaccgccg gcgagcgccg cggtcaggag
     4921 gcgcaggacg gctgaccggg cgccggcggt ggaccggtcg ccggtctcct ccgctgcgat
     4981 gcgccttgcc gccatggtgt cctgccgatg atcgtggatg atcacgttct gcggccttct
     5041 acccacgcac cgcagttatc aaacgctcaa cgtgaccatg aaactgcgtt ccgtgtttgt
     5101 ccagcacgga tctcgagtgg accgtcagcg ccgtccggag ggccgtgccg ctgcccggtc
     5161 ggctgcagcc agctggtccg cgagcgtggc gatcgtcgcg gggtcgtcca cgaggccgat
     5221 ggtgatgcgg acgtgaccgg taccgggacg caccaggaac ggagcgccgg gggcgacgcc
     5281 gatgccctgg gcggccaggg cgacggtggt cgagcgctcg tcggccacgg ggacccactg
     5341 gttgatcccg tcggtcccgg gggcggcgat gccgtggtcg gcgagggcgg cggcgaacgc
     5401 ggcgcggcgg gccgcgtact cgacctgtgc ccgggtgatc tggtcgatgg tccgcccgtc
     5461 gcgcagcagg gcgagcagca cggcctggag gatcctgctg ctccacccgg ggccgaggaa
     5521 ccggcgggtg gccagcgcat cgaccacctc gcccgccccg cccacggccg cgaggcgcag
     5581 gtcggggccg tggctcttcg agaagctccg gatgtgcacg gtctggctgg ggagccaggt
     5641 gccgacgctg atcggcggcg cttcggagat gtcgccgatg tggtcgtcct ccaccaccag
     5701 ggcaccggtg cccttcagca gcttggcgat ggcgcgggcc cgtcgtcccg acgtggtgat
     5761 gccagtgggg ttctgggccc ggggctgcag gaagaccatc gacggcttct gggcgagcgc
     5821 ggcctccagg ctggtgagcg agatgccctc gtcgtcgagg tcgaccccga tgacctcggc
     5881 gccgagctgg tcgagcaggt ccaagacggg tgggaacgtg gggttctcga ccaccacccg
     5941 atccccgagc cggacgatga cctgggccac ccggtcgatg gcgtccatgg ccccgtccac
     6001 caccgtcagg gactcggcct ggaacggcca gacgctccgc agggactcct cgagctgggg
     6061 gaggaccggc gggtcgaggt aggagctggt gaggttctgg cgggcgacga gggcgacgat
     6121 cgggccgagg tcggggagca gcgcagggtc cggcgtgccg gtcgacaagt cgagcgcgaa
     6181 gtgccccgga ccctccgtca cgcgccggta gcggtgcccg gccccggggg cgggcacctg
     6241 gcgcacgaac gtgccgttgc gccccctcgc ctcgatcgcc ccgacggccg agaggatccg
     6301 ccaggcctcg ctgacggtcg tgggggagac gtcgagctcc cttgcgaggg cccggaccgt
     6361 gggcagccgg tcgcccacgg gcaggtcgcc ggcggtgatg aggcggccga tgccggcggc
     6421 gatgccctgg gcgttgcggg tctcgatggc gtcggcgagc gacatggcca gggtgctcct
     6481 cgcgggtcgg tgtcggcgta ttgtacagta acaaataaca gattgtatga agcctgctgt
     6541 gtcattaggg tcgggttcgt gggcgggcct gccgccgtgg cggaccgacc ggaggagcag
     6601 cccgtgaagc gcatcagcca ctggatcgac ggccgggtcg tcgccgggac ctccggccgt
     6661 gaaggcgtgg tgtggaaccc ggccaccggc gagcagcagg ccgccgtcga cctcgcctcc
     6721 gccgctgagc tcgacgccgc ggtcgccgcg gccaaggccg ccttccccgc gtggcgcgcc
     6781 accgggttgt cgcggcgggc cgaggtcatg ttccggctgc gggacctgat cgacgccaac
     6841 cgcgaggaga tcgcgtcgct ggtcaccgcc gagcacggca aggtcctctc cgatgcgctg
     6901 ggcgaggtcg cccgggggct ggagaacgtg gagttcgcca cgggggtgcc gcacctgctg
     6961 aagggcgggt tcaccgagca ggccgcaacc ggggtcgacg tgtacagcat ccgtcagccg
     7021 ctcggcgtgg tggcggggat cacgccgttc aacttcccgg cgatggtccc catgtggatg
     7081 ttcgccaacg cgctggcgtg cgggaacacc ttcatcctca agccgtccga gaaggacccg
     7141 tccaccgcgc tgtacctggc cgagctgctc gcccgggccg gcctgcccgc cggcgtgttc
     7201 aacgtcgtca acggcgacaa ggaggcggtc gacgccatcc tcgccaaccc cgacgtcgcc
     7261 gccgtcagct tcgtgggcag cacgccgatc gcccggacga tctacgagac cggcacccgg
     7321 gccggcaagc gggtccaggc gctgggcggc gccaagaacc acatgctcgt cctgcccgac
     7381 gccgacctcg acatggccgc cgatgcggcg gtgagcgcgg cctacggatc ggc
//
LOCUS       DATMFS010000327         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105620, whole genome shotgun sequence.
ACCESSION   DATMFS010000327 DATMFS010000000
VERSION     DATMFS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105620"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1429
                     /locus_tag="VNQ33_05920"
     CDS             218..1429
                     /locus_tag="VNQ33_05920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ97678.1"
                     /translation="MEFGLFFNGYLPGPAAHDRESEHTMLMRELGYAIHADRFNWKYA
                     WFGEHHALVEYSHMSAPETFFTYVTAQTTQIHVGSGIMNLSRPVNHPVRNAERVAMLD
                     HVTGNRYEWGTGRGAGSHEMATFDLLTSETKAMWDEAAPEIIRMWEQKDYTFEGEFFR
                     VETPHNILPKPYGIGHPPLWVGCGNPGTFTKAGQLGIGAIAFNFEPIYNLRGRIEAYK
                     EGIADCTEPLGQFVNDNVMMTNAVVCVSDRKRAREIAMSAGRGYLNTMVNLYHDTMPP
                     QPGAVKWPNAPFAIPDEATLDWLIEAGYLLCGSPDDVLEQIGKYQEVGCDQLVFGIPN
                     EGFEHEEVLEMLELFGTKVIPEFDGEPVHRTTRMRATAKRKHPDFAHPLPPETGEVTV
                     IPTNALLPLGS"
     gene            1611..2036
                     /locus_tag="VNQ33_05925"
     CDS             1611..2036
                     /locus_tag="VNQ33_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97679.1"
                     /translation="MARRGGPVDAERGRLRAEAGDDPSLADLVRCLVVPYAALLDDAA
                     GRSYLRIVAQLRGRFAAWRVESDAATTQHLAAVLDQIESVPSAPPAIRRERVVALIML
                     LTGAVAERARRIDDGSGNELDHDAFVANLTAMCAAVVTA"
BASE COUNT          340 a          780 c          665 g          295 t
ORIGIN      
        1 cgatgatcca ccacgtcctg ctcttggcat cggtgcggat ggcatcggca tcctcctcgt
       61 tccaggagga ggggagaccg gcgtcgggcg ggggctgggt gtcgctcatg gtccacccag
      121 gttggccggt gggcggaggc agggcaacga ccccttgccc atcgcgcgtc taatgccata
      181 cattagaaca cgttctactt cttgcgagga gcgcaccatg gagttcggcc tgttcttcaa
      241 cggctacctg cccggccccg cggcccacga ccgcgagtcc gagcacacca tgctcatgcg
      301 ggagctcggc tacgccatcc acgccgaccg cttcaactgg aagtacgcct ggttcggcga
      361 gcaccacgcc ctggtcgagt actcgcacat gtccgcaccg gagacgttct tcacctacgt
      421 cacggcgcag accacccaga tccacgtcgg ctccgggatc atgaacctgt cccggccggt
      481 caaccacccg gtccgcaacg ccgagcgcgt cgccatgctc gaccacgtca ccggcaaccg
      541 ctacgagtgg ggcaccgggc gcggtgccgg cagccacgag atggcgacct tcgacctgct
      601 cacgtccgag accaaggcca tgtgggacga ggccgctccc gagatcatcc gcatgtggga
      661 gcagaaggac tacacgttcg agggcgagtt cttccgggtg gagacgccgc acaacatcct
      721 ccccaagccc tacggcatcg gccacccgcc gctgtgggtg ggctgcggca acccgggcac
      781 cttcaccaag gccggccagc tcggcatcgg cgccatcgcc ttcaacttcg agccgatcta
      841 caacctccgc ggccgcatcg aggcctacaa ggagggcatc gccgactgca ccgagccgct
      901 gggtcagttc gtcaacgaca acgtgatgat gaccaacgcc gtcgtctgcg tgagcgaccg
      961 gaagcgggcc cgcgagatcg ccatgtccgc cggccgcggc tacctcaaca cgatggtgaa
     1021 cctgtaccac gacaccatgc cgccccaacc gggtgcggtg aagtggccga acgcgccgtt
     1081 cgccatcccc gacgaggcca cgctcgactg gctcatcgag gccgggtacc tgctgtgcgg
     1141 cagccccgac gacgtgctcg agcagatcgg gaagtaccag gaggtcggct gcgaccagct
     1201 cgtcttcggc atcccgaacg agggcttcga gcacgaggag gtgctggaga tgctggagct
     1261 gttcggcacc aaggtcatcc ccgagttcga cggcgaaccc gtccaccgca ccacccgcat
     1321 gcgggcgacg gcgaagcgca agcacccgga cttcgcccac ccgctgccgc ccgagaccgg
     1381 cgaggtcacg gtcatcccga cgaacgccct cctcccgctg gggagctgac gccacggccc
     1441 ccgtcgccac cgcgacccgc gcccggatcc tcgacgaggc cgagcggctc ttcgccgccg
     1501 gtggcgtcga gggcgtcacc acgcgccaga tcaccgaggc cgccggccag cgcaacacct
     1561 ccgcggtcag ctaccacttc ggctcgcgcc agggcctgct gcgcgagctc ttggcgcggc
     1621 ggggcggacc ggtcgacgcc gagcggggcc ggctgcgcgc cgaggccggc gacgacccct
     1681 cgctggccga tctggtgcgg tgcctcgtgg tcccctatgc cgccctgctc gacgacgccg
     1741 ccggccgctc ctacctgcgc atcgtcgccc agctccgcgg ccggttcgcg gcgtggcgcg
     1801 tggaatcgga tgcggccacc acccagcacc tcgccgccgt gctcgaccag atcgagtcgg
     1861 tcccgtcggc accgcccgcg atcaggcggg aacgggtggt ggcgctgatc atgctgctca
     1921 ccggtgccgt ggccgaacgg gcccgccgca tcgacgacgg cagcggcaac gagctcgacc
     1981 acgacgcctt cgtcgccaac ctcacggcca tgtgcgccgc cgtcgtcacc gcctgagccg
     2041 atcgccaccg gcaaatcagg tcgttcgtgc acctcatagg
//
LOCUS       DATMFS010000328         4702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_265226, whole genome shotgun sequence.
ACCESSION   DATMFS010000328 DATMFS010000000
VERSION     DATMFS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265226"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1349)
                     /locus_tag="VNQ33_05930"
     CDS             complement(30..1349)
                     /locus_tag="VNQ33_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ97680.1"
                     /translation="MVKARFAAMTERLTPEQAAALLHPADILGIPLGPGHPGGFLHAL
                     GARSDWEDLLITGALLTDFYEVFAKPGVRFLSGFYGPLERLMRDQGGAIEFVPADFRR
                     FGPALERINPRVVATAAAPPDADGWCSLSLHAGATVAELSRAAVDPGRILVVEVSPGF
                     PTTLGLGEHRHRIHVDQIDVLVETDREPFVIGDPEPTEDEQAIAAFVDDFVLDGATIQ
                     TGIGGVPSTVASLLAAGDKGDFGIHSEMFTTGLMKLHQAGKVTNNKGIYDGVSITTFA
                     GGTRELYDWLDGNREVRFLPVHLVNSPELISKNRRMISINGALAVDLAGQAVADTLAG
                     AQFSGIGGHEDFVAASGLELEDRSLICLKSSTIVDGVRVSRIAGQFAAGTIVTTLRHQ
                     LDVVITEYGVAELRGRTVRERARALAAIAHPDFRAGLLAQAEVWPPG"
     gene            1400..1993
                     /locus_tag="VNQ33_05935"
     CDS             1400..1993
                     /locus_tag="VNQ33_05935"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWJ97681.1"
                     /translation="MSVRHALLALLSEGPKYGLQLRHEFEARTGEVWPLNVGQVYTTL
                     QRLERDGLVASDGSDDGPQKGYRITGGGRAELTAWLETPPDMAQPPRDELLIKVLVAA
                     QVPEVDVHQLIQVHRRHLVESMQRYTAVKADAPPDDVALALVVDAELFRIEAMVRWLD
                     AAEVRLPQLLAAAPPAAGTDQPVPASEPSSETEAARP"
     gene            1990..2718
                     /locus_tag="VNQ33_05940"
     CDS             1990..2718
                     /locus_tag="VNQ33_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97682.1"
                     /translation="MSPMLELRDVTKTYGEGQGEVHALRSVDLSVEAGELVAIMGPSG
                     SGKSSLLTIAGSLEDATAGEVVIDGRSLAGMSGNDKAQLRRRSMGFVFQDFNLLPGLT
                     AAENVAMPLELDGTSARPARKQALAALDELGLSDRAEHFPDDLSGGERQRVAIARAVV
                     GDRRLLLADEPTGALDSVNSEAVMRLLRQICARGAAGIVVTHDAQLASWANRVVFLRD
                     GRVVDHTAAPPGPETLLATAPGAP"
     gene            2715..>4702
                     /locus_tag="VNQ33_05945"
     CDS             2715..>4702
                     /locus_tag="VNQ33_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWJ97683.1"
                     /translation="MSTARRPSGPPRRAMVRWSWRMFRREWRQQLLVLSLVTVAVAAS
                     VTGSAMAMNAASNTQSSFGTATAMARIEVQDRSEAEDAVATARERYGTVEVIGHTPAA
                     VPGIPLPLDIRAQDPDGTFGAPLLGVREGRYPTADDELALTPDAAELLDAGVGGEVSL
                     GRRSWKVVGLVENPRALSDTFALAAPAGWTSAASLQLLFERPGQGGASASPASGGGEN
                     AGQMPIPVMIRGSNGPVAALVLVAVALLMTLVGLIAAAGFVVLAQRRQRQLGLLAAIG
                     ATQRHLRTVVLAHGAIVGTVAAVVGGVLGIFGWLAAAPAVEQAAGRRIGRLDLPWGVL
                     AGCLVLAVAMAVAAAWWPARRVARIPVIAALSGRPPKPAPVHRSTLLAVVLLAGGMGA
                     IAAALPDGDKVNPLLLMGGVIAVGIGSVFIAPAAVRVAALPARRLPLPSRLAVRDLAR
                     FQSRAASALAAIAFAIGLSVTIVAVAKVNEYRPDEGNLPANQVLVRLGADVDQLPRDP
                     EPGVDQERDADAQEVADAVGASTTFPLDIAMPADHGQPRPEPITQALQVGDHTWRGLG
                     LPYVATSELLRHYGIDPASVHDDTELLTSDDRPVVLADTSQRDDLGPTKVQHVDLPTY
                     FAAPHALVTERAIEDHGWERRRDSWLIETDAPLTTEQI"
BASE COUNT          628 a         1797 c         1656 g          621 t
ORIGIN      
        1 ggcacctcag cgccggttct ggagcgggat caaccaggag gccagacctc ggcctgggcg
       61 agcaggcccg ctcggaagtc cgggtgggcg atggcagcca gcgcccgcgc ccgctcgcgc
      121 accgtgcggc cacggagctc ggcgacgccg tactcggtga tgaccacgtc gagctggtgg
      181 cgcagggtgg tcacgatggt gccggccgcg aactggccgg cgatccgaga cacccgcacc
      241 ccgtcgacga tcgtcgacga cttcaggcag atcagcgaac ggtcctccag ctccagcccg
      301 gaggcggcga cgaagtcctc gtggccgccg atgcccgaga actgggcccc ggcgagcgtg
      361 tcggccacgg cctggccggc gaggtcgacc gccagtgccc cgttgatcga gatcatccgg
      421 cggttcttcg agatcagctc tggcgagttc accaggtgga cgggcaagaa gcgcacctcg
      481 cggttgccgt cgagccagtc gtagagctcc cgggtgccgc cggcgaacgt cgtgatcgag
      541 acgccgtcgt agatgccctt gttgttcgtg accttgccgg cctggtggag cttcatgagc
      601 ccggtcgtga acatctccga gtggatgccg aaatcgccct tgtcgccagc cgccagcagc
      661 gacgccaccg tggacggcac cccgccgatc ccggtctgga tggtggcccc gtccagcacg
      721 aagtcgtcca cgaacgcggc gatcgcctgc tcgtcctcgg tcggctccgg atcgccgatc
      781 acgaacggct cccggtcggt ctcgacgagg acgtcgatct ggtcgacgtg gatccggtgg
      841 cggtgctctc ccagcccgag cgtggtcggg aacccggggc tgacctcgac gacgaggatc
      901 cggccggggt cgacggcggc ccgcgacagc tcggcgacgg tggcgccggc gtggagcgag
      961 aggctgcacc acccgtcggc gtccggcggt gcggctgcgg tggccacgac gcgggggttg
     1021 atgcgctcga gcgccgggcc gaaccgccgg aagtcggcgg gcacgaactc gatggcgccg
     1081 ccctggtcgc gcatcaggcg ttcgagcggg ccgtagaagc ccgataggaa ccggacgccc
     1141 ggcttggcga agacctcgta gaagtcggtg aggagggcgc cggtgatgag caggtcctcc
     1201 cagtcgctgc gggcgcccag ggcgtgcagg aagccgcccg ggtgcccggg gccgagcggg
     1261 atgcccaaga tgtcggcggg gtggagcagg gcggcagcct gctcgggggt gaggcgctcc
     1321 gtcatggcgg cgaacctagc cttgaccatc taagaatact cggtatagta cccggtatgc
     1381 tgatgcgagg agaccaccga tgagcgtgcg ccacgccctg ttggccctgc tgagcgaggg
     1441 gccgaagtac gggctccagc tccgccacga gttcgaggct cggaccggtg aggtctggcc
     1501 gctcaacgtg ggccaggtgt acacgaccct ccagcgcctc gaacgagacg gcctcgtcgc
     1561 ctccgacgga tccgatgacg gaccgcagaa gggctaccgc atcacgggcg gcggccgggc
     1621 cgagctcacc gcctggctcg agaccccgcc cgacatggcc cagccgcctc gagacgagct
     1681 cctcatcaag gtgctcgtcg ccgcccaggt gcccgaggtc gacgtgcacc agctcatcca
     1741 ggtccaccgc cggcacctcg tcgagtccat gcagcgctac accgcggtga aggccgatgc
     1801 cccgcccgac gatgtcgccc tcgccctggt cgtcgacgcc gagctgttca ggatcgaggc
     1861 gatggtccgc tggctcgacg cggccgaggt ccgcctgccc cagctcctgg cggccgctcc
     1921 cccggcggcc gggaccgacc agccggtgcc ggcgtccgag ccgtcgtcag agaccgaggc
     1981 ggcgcgccca tgagcccgat gctggagctg cgcgacgtca ccaagacgta cggcgagggc
     2041 cagggcgagg tccacgccct ccggtccgtc gacctgtccg tggaggccgg cgagctggtg
     2101 gcgatcatgg gcccgagcgg atcggggaag agcagcctgc tgaccatcgc cggcagcctg
     2161 gaggacgcca ccgccggcga ggtcgtgatc gacggtcgct cgctggcggg gatgtccggc
     2221 aacgacaagg cccagctccg ccggcgctcg atgggcttcg tcttccagga cttcaacctc
     2281 ctcccgggcc tgaccgcggc cgagaacgtc gccatgccgc tcgagctcga cggcacgagc
     2341 gcccggcccg cccgcaagca ggcgctggcc gcgctcgacg agctgggtct gtcggaccgc
     2401 gccgagcact tccccgacga cctctccggc ggcgagcgcc agcgcgtcgc catcgcccgc
     2461 gccgtggtcg gagaccgtcg gctcctcctg gccgacgaac cgaccggtgc gctcgactcg
     2521 gtgaacagcg aggcggtgat gcgcctgctc cgccagatct gcgctcgggg agccgccggc
     2581 atcgtggtca cgcacgatgc ccagctcgcc tcgtgggcga accgggtggt gttcctccgc
     2641 gacgggcggg tggtcgacca caccgcggcg ccgccgggcc ccgagacgct cctcgccacg
     2701 gcgcccggcg caccgtgagc accgcccgac ggcccagcgg tccgccccgc cgggcgatgg
     2761 tccgctggtc ctggcgcatg ttccgccgcg agtggcggca gcagctcctc gtcctctccc
     2821 tggtgacggt cgccgtcgcc gcgtccgtca ccgggtcggc catggcgatg aacgccgcct
     2881 ccaacaccca gtccagcttc ggcaccgcca cggcgatggc ccgcatcgag gtccaggacc
     2941 gctccgaggc cgaagacgcc gtcgcgacgg cccgggagcg ctacggcacg gtcgaggtga
     3001 tcggccacac ccccgcggcg gtgcccggca tccccctccc gctggacatc cgggcccagg
     3061 atcccgacgg caccttcggc gcaccgctcc tcggcgtgcg cgagggccgg taccccaccg
     3121 ccgacgacga gctcgcgctc acgcccgatg ccgccgagct gctcgacgcg ggcgtcggcg
     3181 gcgaggtcag cctcggccgg cgctcgtgga aggtcgtcgg cctcgtcgag aacccacggg
     3241 cgctcagcga cacgttcgcc ctggccgccc ctgcggggtg gacgtccgcc gcctcgctgc
     3301 aactgctgtt cgagcgcccc ggccagggcg gggccagcgc cagcccggcg tcaggaggcg
     3361 gcgagaacgc cggccagatg ccgatccccg tgatgatccg cgggagcaac ggccccgtgg
     3421 ccgccctcgt gctcgtcgcc gtcgcgctcc tcatgaccct cgtcgggctc atcgccgccg
     3481 ccgggttcgt cgtcctcgcg cagcgacggc agcgccagct cggtctgctc gccgccatcg
     3541 gggcgacgca gcggcacctg cgcacggtcg tcctcgccca cggcgccatc gtcgggaccg
     3601 tggccgccgt cgtgggcggg gtgctcggca tcttcggctg gctcgcggcg gcgcccgccg
     3661 tcgagcaggc cgcaggtcgg cgcatcgggc ggctcgacct cccgtggggc gtgctcgccg
     3721 gctgcctcgt cctggccgtc gccatggcgg tggccgcggc ctggtggccg gcgcgccggg
     3781 tggcgaggat cccggtcatc gccgccctgt ccgggcgtcc gccgaagccg gcgcccgtgc
     3841 accgctcgac cctcctcgcc gtggtgctcc tggccggcgg catgggtgcc atcgccgccg
     3901 cgctgcccga cggcgacaag gtcaacccgc tgctcctcat gggcggcgtc atcgccgtcg
     3961 gcatcggctc ggtcttcatc gctcccgccg ccgtccgcgt cgccgccctg ccggcgcgcc
     4021 gccttccgct ccccagccgg ctggcggtgc gcgacctcgc ccgcttccag tcccgggctg
     4081 cctcggcgct cgccgccatc gccttcgcca tcggcctctc cgtcacgatc gtggccgtcg
     4141 ccaaggtcaa cgagtaccgg cccgacgagg gcaacctccc ggccaaccag gtcctggtgc
     4201 gcctgggcgc cgacgtcgac cagctcccgc gcgaccccga gccgggcgtc gaccaggagc
     4261 gcgacgccga cgcgcaggag gtcgccgacg ccgtcggggc ctcgaccacc ttcccgctcg
     4321 acatcgccat gccggccgac cacggacagc cccggcccga gccgatcacc caggcgctcc
     4381 aggtcgggga ccacacctgg cgcggcctcg ggctgccgta cgtcgccacg tccgagctgc
     4441 tgcgccacta cggcatcgac cccgcctcgg tccacgacga caccgagctg ctcaccagcg
     4501 acgaccggcc ggtcgtgctc gccgacacgt cccagcggga cgacctcggg cccaccaagg
     4561 tccaacacgt cgacctgccg acctacttcg ccgcacccca tgccctcgtc accgagcgtg
     4621 cgatcgaaga ccacggctgg gagcggcggc gcgacagctg gctgatcgag accgacgcgc
     4681 cgctcaccac cgagcagatc ca
//
LOCUS       DATMFS010000329         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_151570, whole genome shotgun sequence.
ACCESSION   DATMFS010000329 DATMFS010000000
VERSION     DATMFS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151570"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VNQ33_05950"
     CDS             complement(<1..302)
                     /locus_tag="VNQ33_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97684.1"
                     /translation="MTSPRRAARSGYDRVGGSRLTAVGTILAVGVLAGALSPPPATDR
                     QILVLVWASMAAAMAIGVVWPLLAIRRATVEVRSPRDATVGDDVPIEVRVGGRTGA"
     gene            complement(299..1279)
                     /locus_tag="VNQ33_05955"
     CDS             complement(299..1279)
                     /locus_tag="VNQ33_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWJ97685.1"
                     /translation="MTQVSTPPAGGAAPAETREAIELSGRLFEAVASTVRGKDEIVRL
                     SLVALLAGGHLLVEDLPGTGKTLLAKSLAAALGGRFGRVQCTPDLLPTDITGTSVYSA
                     ADGSWTFRPGPLFANVVLVDEINRASPRTQAALLEPMEEHQVSVDGLTHPLPDPFFCV
                     ATQNPHGQIGTFPLPESQLDRFALVLSMGLPDRAAEREILTGRGGNDAYSDIEAVADP
                     ARVAAAVATVHTVYCAPALVEYVLDLAAATRSHPDLAVGASPRASVGLLQAARAHAVV
                     VGREHVTPDDVQAVIGPAFAHRISVGGAVDTTAGAAVVADIVARTAVPRP"
     gene            complement(1347..2399)
                     /locus_tag="VNQ33_05960"
     CDS             complement(1347..2399)
                     /locus_tag="VNQ33_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWJ97686.1"
                     /translation="MDIRGTRKQGMAALAAVAALAVVAGASLAACSSASDSGGAADEP
                     TSTSTTAAGEASGSGEFSLLSYNVAGLPAEISKEHPDVNIPKISPHLDDYDVVMTQED
                     FDWWKPGGLADGLDFNHYHERLQADAHHEYQTERHPGPEAVGIDIEADRPNMELGDGL
                     GVLSVDPISDTVRVPWDSCFGEFDSGASDCLAMKGFLMTRLELADGVVVDLYDLHGEA
                     GGSAEDQALQVKDYEQLAAYVNEHSEGHAVILGGDTNLHTDPPGGDAHEDSANGEDLQ
                     IWNDFLEATGLTDACEATDCDDPGRIDKIAFRSDDGIELTPTSHEFQTDVFVDEHGED
                     LSDHQALEVVFRWKQA"
     gene            complement(2474..3502)
                     /locus_tag="VNQ33_05965"
     CDS             complement(2474..3502)
                     /locus_tag="VNQ33_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97687.1"
                     /translation="MVRPSLARWSAAVSFLALGSALAACSNPSESTVSADAVPTTIDI
                     ADNPNGGSFRLLTYNVQGLPVEISEGRPDLNLPSISPLLNDYDLVLTQEDYDWWKPEG
                     LASGFDFVNYHGRLRAKATHEYQSPQHPGPEAVGVDLAERPDLELGDGLGILANVDVE
                     GDERVPWTGCFGGFSQDDGGKADCLAMKGFQVTTLTLANGAPVHVYNLNAESGTNPKD
                     QQLQAADFEQLAEHIAHHAQGKAVIVAGHTNLALDDADPASGRGQDRRIWEHFLEQAG
                     LTDACEAGTCDDAVIDRVVYRSGGGVELSASSQVDRSDRFVDDAGEPLSGFPPLEVAF
                     DWRPEGSS"
     gene            complement(3586..3720)
                     /locus_tag="VNQ33_05970"
     CDS             complement(3586..3720)
                     /locus_tag="VNQ33_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97688.1"
                     /translation="MDIALVVVVVAVLVVVQIRRRGSRLQRAEELRRSADLPRKTDPG
                     "
     gene            3819..>5303
                     /locus_tag="VNQ33_05975"
     CDS             3819..>5303
                     /locus_tag="VNQ33_05975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWJ97689.1"
                     /translation="MGTEHVLAIDLGTGGPKAALVTPEGRITAHEFEPTPLELLGDGG
                     AEQDPDGWWAAITTAVRRLLDGGHVAADDIVAVSVTSQWCGTVAVDAGGKHLYPAVIW
                     MDSRGAPYVEELVTGRLNVAGYDAFRLKSWIQKTGGIPSHSGKDPIAHILWLKHHQPD
                     VYRATDTFLEPCDYLNARLTGRICASYDSITAHWLTDNRDLSHVRYDPQLLGWCGLTE
                     AQLPELVPSASVVGTVLPAVAEEWGLTPATKVVTATGDVHSAVVGSGALGDFEGHLYM
                     GTSSWLSCHIPTKRTDLLNNQAALPSAVPGRYFLANEHEVGGGALLWLRDQAQMIRDV
                     DEANELAETAAPGSGRVIFTPWLNGERTPVDDHTIRGGWNNVSLTTTRPELVRSVFEG
                     VAFNSRWLLDTVEKFVKRPLDGLNFIGGGARSDLWCQMHADVCDRVIRRVAEPQHANT
                     RGAAFVAWLALGRQTLDELNRAVTIEREFHPDPDGRAAYAPLYKE"
BASE COUNT          741 a         1892 c         1917 g          753 t
ORIGIN      
        1 caggcgccgg tgcggccgcc cacgcgcacc tcgatgggca cgtcgtcgcc gacggtcgcg
       61 tcacgtggcg accgcacctc gaccgtggcg cgccggatgg ccagaagcgg ccacaccacc
      121 ccgatggcca tcgcggccgc catgctcgcc cacaccagca ccaggatctg gcggtcggtc
      181 gccggcggcg ggctcagggc cccggcgagg acgccgacgg cgaggatcgt gccgaccgcg
      241 gtgagccggg aaccgccgac gcggtcgtag ccggagcgtg ccgctcggcg aggcgaggtc
      301 acggccgcgg cacggccgtc cgggcgacga tgtcagcgac gacggcggcc ccggccgtgg
      361 tgtccacggc acccccgacg ctgatccggt gggcgaacgc cgggccgatg acggcctgga
      421 cgtcgtcggg cgtcacgtgc tcgcggccca cgacgaccgc gtgggcccgg gccgcctgga
      481 gcaggcccac cgatgcccgg ggcgacgcac cgacggccag gtcggggtgg gaccgggtgg
      541 cggccgcgag gtccagcacg tactcgacga gggcaggggc gcagtagacg gtgtggaccg
      601 tggcgacggc ggcggcgacc cgggcgggat cggcgaccgc ctcgatgtcc gagtaggcgt
      661 cgttgccgcc gcgacccgtc aggatctcgc gctcggccgc ccggtcgggc aggcccatcg
      721 acaagaccag cgcgaaccgg tcgagctggc tctccggcag ggggaacgtg ccgatctggc
      781 cgtgcgggtt ctgggtcgcg acgcagaaga acgggtcggg cagcggatgg gtcaggccgt
      841 cgacggagac ctggtgctcc tccatgggct cgagcagggc ggcctgggtc cgtggcgagg
      901 cccggttgat ctcgtcgacg agcacgacgt tggcgaacag cggaccgggc cggaaggtcc
      961 acgacccgtc ggccgccgag tacacggacg tgccggtgat gtcggtgggg agcaggtcgg
     1021 gcgtgcactg cacccggccg aagcgcccgc cgagggccgc cgcgaggctc ttggcgagga
     1081 gggtcttgcc ggtgcccggc aggtcttcca ccagcaggtg gccgccggcc agcagggcga
     1141 cgaggctcag ccggacgatc tcgtccttgc cgcgcacggt cgacgcgacg gcctcgaaca
     1201 gccgacccga cagctcgatc gcctcgcgcg tctcggccgg cgcggcgccg ccggcgggtg
     1261 gggtgctcac ctgggtcatg cggtcgaagc cttgcagctc cggcgcggcg gtggaaggcg
     1321 gtcggccccg gctcccggcg ggaggatcag gcctgcttcc agcggaacac gacctcgagg
     1381 gcttggtggt cgctcaggtc ctcgccgtgc tcgtcgacga agacatcagt ctggaactcg
     1441 tggctcgtcg gtgtgagctc gatgccgtcg tcggaccgga aggcgatctt gtcgatgcgg
     1501 cccgggtcat cgcaatcggt ggcctcgcag gcatcggtca acccggtggc ctcgaggaag
     1561 tcgttccaga tctggaggtc ctcgccgttc gcgctgtcct cgtgggcgtc gccgccgggc
     1621 gggtcggtgt ggaggttggt gtcgccaccg aggatcacgg cgtggccctc gctgtgttcg
     1681 ttgacgtagg cggcgagctg ctcgtagtcc ttcacctgga gcgcctggtc ctcggcactg
     1741 ccgccggcct cgccgtggag atcgtagagg tcgacgacca cgccgtcggc cagctcgagg
     1801 cgggtcatca agaacccctt catcgcgagg cagtccgatg cgcccgagtc gaactccccg
     1861 aagcacgagt cccacggcac gcgcacggtg tcggagatcg ggtcgaccga cagcacgccg
     1921 aggccgtcgc ccagctccat gttgggtcgg tcggcctcga tgtcgatgcc cacggcctcc
     1981 gggccggggt gccgctcggt ctggtactcg tggtgggcgt cggcttgcag gcgctcgtgg
     2041 tagtggttga agtcgaggcc gtcggcgagg ccacccggct tccaccagtc gaagtcctcc
     2101 tgcgtcatca cgacgtcgta gtcgtcgagg tgcgggctga tcttcgggat gttgacgtcg
     2161 gggtgctcct tggagatctc cgcgggcagc cccgccacgt tgtacgagag caggctgaac
     2221 tcacccgacc cgctggcctc gcccgccgcg gtcgtggacg tggacgtggg ctcgtcggcc
     2281 gcgcccccgc tgtcgctcgc gctgctgcag gcggcgagcg atgccccggc caccaccgcc
     2341 agcgcggcca ccgccgcgag cgcggccatc ccctgcttcc tcgtccccct gatgtccatg
     2401 acaggcgagc ctagaagccc gcggccacct cgccgacacc cgcggttcgc ctggggttca
     2461 cccgaggggc cgatcagctc gagccctcgg gcctccagtc gaacgcgacc tcgaggggcg
     2521 gaaacccgct gagcggctcg ccggcgtcat cgacgaaccg gtcgctgcgg tccacctgcg
     2581 acgacgcgct cagctcgacg ccgcctcccg agcggtagac gacccggtcg atgaccgcgt
     2641 cgtcgcacgt gccggcctcg cacgcgtcgg tcaggccggc ctgttcgagg aagtgctccc
     2701 agatgcggcg gtcctgaccc cgcccgctgg ccgggtcggc atcgtcgagc gcgaggttgg
     2761 tgtggccggc gacgatcacg gccttgccct gcgcgtggtg agcgatgtgc tcggcgagct
     2821 gctcgaagtc ggccgcctgg agctgctggt ccttggggtt cgtcccggac tcggcgttga
     2881 ggttgtagac gtggaccggt gcgccgttcg ccagcgtgag cgtcgtcacc tggaagccct
     2941 tcatcgccag gcagtccgcc ttgcccccgt cgtcttggct gaagccgccg aagcaccccg
     3001 tccacgggac gcgctcgtcg ccctcgacgt cgacgttggc gaggatgccc aggccgtcgc
     3061 ccagctccag gtccggccgc tcggcgaggt cgacccccac cgcctcgggg cctggatgct
     3121 gcggcgactg gtactcgtgg gtggccttgg cccggaggcg gccgtggtag ttcacgaagt
     3181 cgaagcccga tgccaggccc tcgggcttcc accagtcgta gtcctcctgc gtgaggacga
     3241 ggtcgtagtc gttcaggagc gggctgatcg acggcaggtt gagatcgggc cggccctcgg
     3301 agatctccac cggcaggccc tgcacgttgt acgtgagcag gcggaacgat ccgccgttgg
     3361 ggttgtcggc gatgtcgatc gtggtgggga cggcatcggc ggacaccgtc gactcggacg
     3421 ggttgctgca cgcggcgagg gcggacccga gcgcgaggaa ggagacggcg gcggaccagc
     3481 gggccaggga ggggcggacc atggcctcct atcggcagac aacgttccgt ggttgagtca
     3541 ctactctgcg catttggatg acggcaccgt cacctgacgc agacgtcagc ccgggtcggt
     3601 cttgcggggg agatcggcgg accggcgcag ctcctcggcc cgttggaggc gcgagccccg
     3661 gcggcggatc tgcacgacga cgaggaccgc cacgacgacg accacgagcg cgatgtccac
     3721 gccgccagca tcgcgcccgc caccggggtc aggcgaggtg agggtcgggc cgggtccact
     3781 cactatcgtg ctggcgccgt ctggaagatg aggaccacat gggaaccgag cacgtcttgg
     3841 ccatcgacct ggggacggga gggccgaagg ccgctctcgt gacaccggaa gggcgcatca
     3901 ccgcccacga gttcgagccc acaccgctgg agctgctcgg cgacggcggc gccgagcagg
     3961 accccgacgg ctggtgggcc gccatcacca ccgccgtgcg ccggctgctc gacggcggcc
     4021 acgtcgcggc cgacgacatc gtcgccgtgt cggtcacgag ccagtggtgc ggcaccgtcg
     4081 ccgtcgatgc cgggggcaag cacctctacc ccgcggtgat ctggatggac tcgcggggcg
     4141 cgccctacgt cgaggagctg gtgacagggc ggctcaacgt ggccggctac gacgccttcc
     4201 gcctgaagtc ctggatccag aagacgggcg gcatcccgag ccactcaggc aaggacccca
     4261 tcgcgcacat cctctggctg aagcaccacc agcccgacgt gtaccgggcc accgacacgt
     4321 tcctcgagcc gtgcgactac ctgaacgccc ggctcaccgg tcgcatctgc gcgtcgtacg
     4381 actcgatcac cgcccactgg ctcaccgaca accgcgacct gagccacgtc cgctacgacc
     4441 cgcagctgct cgggtggtgc gggctcaccg aagcccagct ccccgagctg gtcccgtcgg
     4501 cgtcggtggt cggcacggtc ctgccggccg tcgccgagga gtggggcctc acgccggcca
     4561 ccaaggtggt gacggccacg ggcgacgtgc actcggcggt cgtcggctcc ggcgccctcg
     4621 gtgacttcga gggccacctc tacatgggca ccagctcctg gctgtcgtgc cacataccca
     4681 ccaagcgcac cgacctgctc aacaaccagg ccgcgctccc ctccgccgtg cccggccgct
     4741 acttcctggc caacgagcac gaggtcggcg gcggcgccct gctctggctg cgcgaccagg
     4801 cccagatgat ccgcgacgtc gacgaggcca acgagctggc cgagaccgcc gcccccgggt
     4861 cgggccgggt catcttcacg ccgtggctca acggcgagcg cacccccgtc gacgaccaca
     4921 ccatccgagg cggctggaac aacgtgtcgc tcaccaccac ccggcccgag ctggtgcgct
     4981 cggtgttcga gggcgtggcg ttcaacagcc gctggctgct cgacaccgtc gagaagttcg
     5041 tcaagcgacc gctcgacggc ctgaacttca tcggcggcgg cgcccgctcc gacctctggt
     5101 gccagatgca cgccgacgtc tgtgaccggg tcatccgccg ggtcgccgag ccgcagcacg
     5161 ccaacacccg cggcgccgcc ttcgtggcgt ggctggccct cggccgccag accctcgacg
     5221 agctgaaccg ggccgtcacc atcgagcgcg agttccaccc cgaccccgac ggccgggccg
     5281 cgtacgcgcc gctctacaag gag
//
LOCUS       DATMFS010000330         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_172600, whole genome shotgun sequence.
ACCESSION   DATMFS010000330 DATMFS010000000
VERSION     DATMFS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172600"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..665)
                     /locus_tag="VNQ33_05980"
     CDS             complement(42..665)
                     /locus_tag="VNQ33_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWJ97690.1"
                     /translation="MEATCWSDYLCPWCYVGQDRSAQMEALGLTVVHRPYELHPEIPA
                     EGRRIRPDGRLAPTFTRIAAACDEIGLPFRPPMRMPNTRRALETAAWVRVHHPASAGA
                     VHAGLFDAHFAAGLAIDDADVLDQIVADAGGPADEVRAAIAAGAAGPLVDASMAEARS
                     AGVSSTPTWVLEDGFAIPGALDRATLARWITKIVARRPADPSSAPNA"
     gene            709..1479
                     /locus_tag="VNQ33_05985"
     CDS             709..1479
                     /locus_tag="VNQ33_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWJ97691.1"
                     /translation="MRLATWNVNSLKARLLRVEAWLADVEPDVVCLQETKLADGAFPA
                     MAFQSMGYESVHHGEGRWNGVAILSRVGLEDPLFGFGPDDDDPEARLVWATCGGIRIA
                     SAYVPNGRSLDHEHYQYKLAWLAKLRTLLDQQGTAADPLVVAGDFNIAPTDDDVYDAA
                     KFVDVTHTSPAERAALADLESWGLVDTFRRLQPEPKLFSWWDYRAGDFHNHRGMRIDL
                     VLGTEAVAGRATFATIDRQARKGEKPSDHAPVIIDLAD"
     gene            1511..2263
                     /locus_tag="VNQ33_05990"
     CDS             1511..2263
                     /locus_tag="VNQ33_05990"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ97692.1"
                     /translation="MNEQTPPSEYLPKMHGEADPERLAMVTKRLRAAAAGDLSPRRES
                     LKELADAARDLVDRLVATDAPDDVIAAATAEVRAAADRFEGFHQGSLYGFSEMANAGG
                     REDPLYDHSPLIGIANPLAPPMTIREEDGIIVAEVLFGQAYEGPPGCVHGGYVAAAFD
                     ELLGATQSLSGAPGMTGTLTVRYESPTPLKTLLRMEGRLVGVERRKIFTEGSIIVDGR
                     VTATAKGTFIAMQPGQFLDLIAEREARGETLR"
     gene            complement(2260..>2821)
                     /locus_tag="VNQ33_05995"
     CDS             complement(2260..>2821)
                     /locus_tag="VNQ33_05995"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HWJ97693.1"
                     /translation="PIRSPAPDHRDPRGDPVRFEISQDFRHERRAVDEAYADPALYPT
                     LVGLPNLGGMEVRSAERDGDTVRLKIHTLFVREFSPAVAAAIDPSKLSWTQDSTFDLA
                     AGTATFRFLPDNYADRFSAEGRTRTEAKGDGAVRTLSGEVKVRAPLVGGRVEKAIIEG
                     LEEYLAAEAALVDAFIDGSSARADAS"
BASE COUNT          399 a         1014 c         1025 g          383 t
ORIGIN      
        1 ggtggagggc cgggcaggcg gcccggccct ccagccgcgc ttcaggcgtt cggcgccgac
       61 gaggggtcag ctggcctccg ggccacgatc ttggtgatcc accgagcgag cgtggcccga
      121 tcgagggcac cggggatggc gaagccgtct tcgagcaccc aggtgggagt cgatgacacg
      181 ccagccgagc gagcctcggc catcgaagcg tcgacgagcg gtcctgccgc gcccgcagcg
      241 atggcggcgc ggacctcgtc tgcgggccca ccggcatcgg ccacgatctg gtcgagcaca
      301 tccgcatcat cgatcgcgag ccccgccgcg aagtgggcgt cgaacaggcc cgcgtgcacc
      361 gcgcccgcgg atgccgggtg gtgcacccgg acccatgctg cggtctccag cgcccgccgg
      421 gtgttcggca tgcgcatcgg cggccggaac ggcaggccga tctcgtcgca cgccgcggcg
      481 atgcgggtga aggtcggggc cagccggccg tcggggcgga tgcgccggcc ctcggccggg
      541 atctccgggt gcagctcgta cgggcggtgc accacggtga gcccgagggc ctccatctgg
      601 gcgctgcggt cctggcccac gtagcaccac gggcacaggt agtcgctcca gcaggtggcc
      661 tccatgcgcc ggatcgtagg cccgtcgtct cccgctatgg tcggccgcgt gcgcctcgcg
      721 acctggaacg tcaactcgct gaaagcccgg ctgctccggg tcgaggcctg gctggccgat
      781 gtcgagcccg atgtcgtctg cctccaggag accaagctcg ccgacggcgc cttcccggcg
      841 atggccttcc agtcgatggg ctacgagtcg gtccaccacg gcgagggccg gtggaacggc
      901 gtcgccatct tgagccgcgt ggggctcgaa gatccgctgt tcggcttcgg tcccgacgac
      961 gacgatcccg aggcccggct ggtgtgggcc acgtgcggcg gcatccggat cgccagcgcc
     1021 tacgtgccca acggccgttc gctcgaccac gagcactacc agtacaagct ggcctggctg
     1081 gccaagctcc gcacgctcct cgaccagcag ggcaccgcgg ccgaccccct cgtggtcgcg
     1141 ggcgacttca acatcgcccc caccgacgac gacgtgtacg acgccgccaa gttcgtcgac
     1201 gtcacccaca cgagccccgc cgagcgcgcc gcgctcgccg acctggagtc gtgggggctg
     1261 gtcgacacgt tccgccgcct gcagcccgag ccgaagctgt tcagctggtg ggactaccgc
     1321 gccggcgact tccacaacca ccgcggcatg cgcatcgacc tggtgctcgg gaccgaagcc
     1381 gtcgccgggc gggccacgtt cgccacgatc gaccgccagg cgcgcaaggg cgagaagccg
     1441 tcggaccatg cgccggtgat catcgacctg gcggactgaa cccggccgcc cccgaacccg
     1501 gagcaccacc gtgaacgagc agaccccacc gtcggagtac ctcccgaaga tgcacggcga
     1561 ggccgatccg gagcggttgg cgatggtcac caagcgcctg cgggccgcgg ccgccgggga
     1621 cctgtcgccg cggcgggagt cgctgaagga gctggccgac gcggcgcggg atctggtcga
     1681 ccggctcgtg gcgaccgatg cgcccgacga cgtgatcgcg gccgccaccg ccgaggtgcg
     1741 cgccgccgcc gatcggttcg agggcttcca ccagggctcg ctctacggct tctccgagat
     1801 ggccaacgcc ggaggtcgcg aggatccgct ctacgaccac agcccgctga tcggcatcgc
     1861 caacccgctg gctccgccga tgaccatccg cgaggaggac gggatcatcg tggccgaggt
     1921 cctgttcggg caggcgtacg agggtccgcc gggctgcgtg cacggcgggt acgtggcggc
     1981 ggccttcgac gagctgctcg gagccacgca gtccctgtcg ggcgccccgg gcatgaccgg
     2041 cacgctgacc gtccgctacg agtcgcccac gccgctcaag acgctgctgc ggatggaagg
     2101 ccggctcgtg ggcgtcgagc gccggaagat cttcaccgag gggtcgatca tcgtcgacgg
     2161 ccgggtcacg gccacggcga agggcacgtt catcgccatg cagcccgggc agttcctgga
     2221 tctcatcgcc gagcgcgaag cgcgcggcga gacgctgcgc taggaggcgt cagcgcgagc
     2281 cgagctgccg tcgatgaacg cgtcgacgag cgcggcctcg gcggcgaggt actcctcgag
     2341 gccttcgatg atcgccttct ccacgcggcc gcccaccagg ggagcgcgca ccttcacctc
     2401 gccgctgagc gtgcggaccg ccccgtcgcc cttcgcctcc gtccgggtcc ggccctcggc
     2461 ggagaagcgg tcggcgtagt tgtccgggag gaagcggaac gtcgccgtgc cggcggccag
     2521 gtcgaacgtc gagtcctggg tccacgagag cttgctcggg tcgatcgcgg cggcgacggc
     2581 cggcgagaac tcccgcacga agagggtgtg gatcttcagc cgcacggtgt cgccgtcgcg
     2641 ctcggcgctg cggacctcca tgccgccgag gttcggcagc ccgacgaggg tggggtagag
     2701 cgccgggtcc gcataggcct cgtcgacggc tcggcgctcg tggcggaagt cctggctgat
     2761 ctcgaagcgc actgggtctc cccgcgggtc gcggtggtca ggtgccggtg agcggatcgg
     2821 c
//
LOCUS       DATMFS010000331         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_12080, whole genome shotgun sequence.
ACCESSION   DATMFS010000331 DATMFS010000000
VERSION     DATMFS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12080"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..2198
                     /locus_tag="VNQ33_06000"
     CDS             339..2198
                     /locus_tag="VNQ33_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWJ97694.1"
                     /translation="MDIYVGAIGPGADALRTAVGERIASAGLPHRAVPLPSATTSAWT
                     DAGELAFVDTDELEILFDGYLHGHGHPTVEEHLPILAERVVQGRAPLAGRESGIFNLV
                     VRDKRTGAIHLASDPSALFPLYFGRLDDGFFFGSHLYLTAKALGAAPDLLGTVFLVLE
                     QFALGSRTYFEGVERLCAGEVVTFDARTGTVDRRYPEIYFDTYTDLGPDTVDELWNAL
                     LDAARPEVEAGSSVGIMLSEGFDSRLVAGAYQELGADLHTFTHGTEGTVGTTITREVA
                     DRLGSSHRFEPLAEGFPSDLDQLRRQLLLSDNLHIPFWSHGADHFRAAGVSVTTTGYA
                     LDTTLGAHAFAAPSGSSARRALDRYRDIVRQDLGRVTNGDIESVAAQVLASTRVLDLV
                     ELGHRTRRFLAPELAAEVVTRIDDVPDAITAEQERLRRAGATLDSQVVQRYFLENRVR
                     RFSFGQELTLRTANRVVVPCYEPAFLRPMSRIHPRHRLNHKLYLQLLRRHAPGLARIR
                     SGAHGLPPTYPRIALETGRFGWKAYEDREIDRYMAAGDPAATPNIRRVLFPEETAHRA
                     STFAIERLFDRETMVINQESMQLTVDKVRRSEIRVYPQSFYLGLELTQLFDEL"
     gene            complement(2225..2800)
                     /locus_tag="VNQ33_06005"
     CDS             complement(2225..2800)
                     /locus_tag="VNQ33_06005"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWJ97695.1"
                     /translation="MHGGRRISSSTGIDVDLTADERGMGAALAVARRGLDRGELPIGA
                     VVVADGQVVGSSHTEEVTQRRLLVHAELLALEEADRHLGWDRRSATLYTTLEPCLMCV
                     GAAATALVGRVVWAVASDSSLAGRVCRDGEARRSPELPQLHLPELVGPVGEEAASELF
                     RTYVDARGDSCDPFVRWAATLIGHERAVVSR"
BASE COUNT          446 a         1171 c          977 g          413 t
ORIGIN      
        1 ttcggctgga gcctcgtccg cccgaaggac gagacccctc ggatgtgcac ggcggacgga
       61 tacatcaccg agtccggcga gatcctcggc cgcgatctcg accagggttg cgcctgggtc
      121 gatggcgacg gcaacaaggt ccccgtcgac gagaacggcc agccgaccgg ctgatcccga
      181 ccgctcggcc acgctggcgg cggctcgccg gcccggggcg gcgcggccac gatctcctgc
      241 cgccgccggc agctgacgcg gaccgggctc acccgaccag ggacccacta ccatcgcccg
      301 acgggtccgg ggcggacacc gatgagaaga ggggaacggt ggacatctac gtgggggcga
      361 tcggaccagg cgccgacgca ctgcggaccg ccgtgggcga gcgcatcgcc tccgccggcc
      421 tcccccaccg agcggtcccg ctgccatcgg cgacgacatc ggcgtggacc gacgccggcg
      481 agttggcctt cgtcgacacc gatgagctgg agatcctgtt cgacggctac ctgcacggcc
      541 acggacaccc caccgtcgag gagcaccttc cgatcctggc cgagcgggtc gtgcagggcc
      601 gggccccgct cgcgggacgc gaatcgggca tcttcaacct ggtggtgcga gacaagcgca
      661 ccggcgccat ccacctcgcg tcggacccga gcgcgctctt cccgctctac ttcgggcgcc
      721 tcgacgacgg cttcttcttc ggctcgcacc tgtacctgac cgccaaggcc ctgggtgccg
      781 ctcccgatct cctcggcacc gtcttcctcg tgctggagca gttcgcactg gggtcgcgca
      841 cctacttcga aggcgtcgag cgcctctgcg ccggggaggt cgtgacgttc gatgcccgga
      901 cgggcaccgt cgaccgccgc taccccgaga tctacttcga cacctatacc gacctcggac
      961 ccgacaccgt cgacgagttg tggaacgcgc tcctcgacgc cgcccggccg gaggtcgagg
     1021 ccggctcgtc ggtggggatc atgctgtccg aggggttcga ctcgcgcctc gtggccggtg
     1081 cctaccagga gctcggagcc gacctccaca cgttcaccca cggcaccgag ggaaccgtcg
     1141 gcacgacgat cacccgcgag gtggccgatc gcctcggatc gtcgcaccgc ttcgaaccgc
     1201 tcgccgaggg gttcccctcc gacctcgatc agctgcgccg ccagctcctc ctgagcgaca
     1261 acctccacat ccccttctgg agccacggcg cggaccactt ccgcgccgcc ggcgtgtcgg
     1321 tcaccaccac cggctacgcc ctcgacacca cgctcggggc ccatgccttc gccgcaccca
     1381 gcggcagcag cgcgcgccga gcgctcgacc gctaccgcga catcgtgcgc caggatctgg
     1441 gaagggtgac gaacggcgac atcgagtccg tggcggctca ggtgctcgcc tcgacccggg
     1501 tcctggacct cgtcgagctc ggccaccgga cccgccggtt cctcgctccg gagctggccg
     1561 ccgaggtggt gacccggatc gacgacgtgc ccgacgccat caccgccgag caggagcgcc
     1621 tccgacgcgc cggcgccacg ctcgattcgc aggtggtgca gcgctacttc ctggagaacc
     1681 gggtccgccg gttctcgttc ggccaggagc tgaccctccg caccgccaac cgcgtcgtgg
     1741 tgccctgcta cgaaccggcg ttcctgcggc ccatgagccg gatccacccg cgccaccggc
     1801 tcaaccacaa gctctacctc cagctcctgc gccgccacgc gccgggcctg gcccgcatcc
     1861 gaagcggagc ccacggtctc cccccgacgt acccccgcat cgcgctcgag accggccggt
     1921 tcggctggaa ggcctacgag gatcgtgaga tcgaccgcta catggcggcg ggcgacccgg
     1981 ccgccacgcc caacatccgg cgcgtgctct tccccgagga gacggcgcac cgagcctcca
     2041 ccttcgccat cgagcgcctc ttcgaccgcg agacgatggt catcaaccag gagtcgatgc
     2101 agctcacggt ggacaaggtc cgccgctccg agatccgcgt ctacccccag tcgttctact
     2161 tgggcctcga gctgacccag ctcttcgacg agctctgaac ctcgggcctg ctcctccgcc
     2221 ggcttcagcg ggagacgacg gcccgctcgt ggccgatcaa ggtggcggcc caccgcacga
     2281 acgggtcgca gctgtcgcca cgggcgtcga cgtaggtgcg gaacagctcc gatgccgcct
     2341 cctcgccgac cggcccgacg agctcgggga ggtggagctg gggcagctcc ggcgaccgcc
     2401 tggcctcacc gtctcggcag acgcggccgg caaggctcga gtccgacgcg accgcccaca
     2461 ccacccgccc gacgagggcc gtcgccgcgg cgccgacgca catcaggcac ggttccagcg
     2521 tggtgtagag cgtcgcgctg cgccggtccc accccaggtg gcgatccgcc tcctccaggg
     2581 ccagcagctc ggcgtgcacc aggagccggc gctgggtcac ctcttcggtg tgcgagctgc
     2641 ccaccacctg gccgtcggcc acgaccaccg cgccgatggg cagctcgcct cgatcgaggc
     2701 cccgccgggc gacggcgagg gccgcaccca tcccccgctc gtcggcggtc agatcgacgt
     2761 cgatcccggt cgagctcgag atcctgcggc caccgtgcat gttcgggttt ccctacggct
     2821 gtcggtcccc ccgaccacgc tccgcggcgt gaccaaccca gttctacccg gtgatccgga
     2881 cttcgagacg cgcagaaccg gaaaatcacg acggagggtg aacgtccggg atccagccgt
     2941 cgagggcggc gagcggggcg accgcgcccg ggacccagcc cggcgacggg cctactcctt
     3001 gtcgaag
//
LOCUS       DATMFS010000332         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_105898, whole genome shotgun sequence.
ACCESSION   DATMFS010000332 DATMFS010000000
VERSION     DATMFS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105898"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VNQ33_06010"
     CDS             <1..1546
                     /locus_tag="VNQ33_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97696.1"
                     /translation="AGLGLALGLKALLAGFGLDLPGDGLVVETAPMAIAFAIGMVATL
                     VAATIPAIRSSRIAPIEALRDAAAESTAVSKVRAGIGVALAALGTFVMVTATSSADGA
                     MGKAGLGALLIVIAAVTLGPVVARPAAAFVGAPVAGTRGLVGQLARRNAMRNPRRTSG
                     AALALVIGAAVVALFATFGSSLSRSIDETVSQSFGGDLVVMQDGFSSSGLSPKMATAI
                     DQLPEVSETVGLSNVVATVDGTTVYPTAGDPAAMANLLDLDVQQGDLAKLRPGQVAVT
                     EGYATDEGLAVGDDLTMDFADGEQTTFEVGAVYGVGELLGDIVMDQADWTPHELRPGN
                     VAVLVGLADGTTIEQGRQAVEAVAKEYGNPKVEDRDQYIDRVAGQVNQLLTLVYGLLV
                     LAILIALIGLANTLSLSIHERSRELGLLRAVGLTRGRLRAMVRWESVITAVVGTVVGL
                     GVGTFLGWGLVRALSAQEGFVSFQLPTATLVVVLVLSIGAGVLAAVRPAHRAAKLDVL
                     AAIAEP"
     gene            1568..>2582
                     /locus_tag="VNQ33_06015"
     CDS             1568..>2582
                     /locus_tag="VNQ33_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HWJ97697.1"
                     /translation="MTDRNVSDTMPLLLRPWGDVRRLWRCLAYLAVGVPLGIVSFTTV
                     FTLTVLTLSLLITLVLAIPAAWGLFIAARILGSIDRSRAASLLGMELVDPVPPLQGRN
                     WFRRLWERVRTRSRWREIVNAFLLFPVSLACWLTTATTWAGSFALLAMPLYAGHLPGG
                     TAKFWLFEVGPGPGSLAAAVAGLGGLLVVAPWVTIGAANLQSGMTRFLLGRTTEAEMA
                     RLLQRAEEQRSSAVDSAETERRRIERDLHDGAQQRLVALAAELGAAREKLDEDPEAGK
                     ELVAAAHEEAKAALKDIRDLVRGIHPVILEDRGLDAALSAVVARAPVPVHLRVRVEPR
                     PAPA"
BASE COUNT          298 a          980 c          927 g          377 t
ORIGIN      
        1 ggccggtctg ggcctggccc tggggctcaa ggcgctgctg gcgggcttcg gcctcgacct
       61 gcccggcgac gggttggtcg tcgagaccgc accgatggcg atcgccttcg cgatcggcat
      121 ggtcgccacc ctcgtggccg ccacgatccc ggccatccgg tcgtcgcgga tcgcgccgat
      181 cgaggcgctc cgcgatgccg ccgccgagtc gaccgccgtc tccaaggtgc gcgccggcat
      241 cggcgtggcc cttgccgccc tggggacgtt cgtcatggtc accgccacca gcagcgccga
      301 cggcgcgatg ggcaaggcgg gcctcggcgc cctgctgatc gtgatcgccg ctgtcacgct
      361 cggcccggtg gtcgcccgcc cggccgccgc cttcgtgggt gccccggtgg ccggcacccg
      421 cggcctggtc ggccagctgg cccggcgcaa cgccatgcgc aacccgcgcc gcaccagcgg
      481 tgccgccttg gccctggtga tcggcgccgc cgtggtggcc ctcttcgcca ccttcggctc
      541 gtcgctgagc cgctcgatcg acgagaccgt gtcgcagagc ttcggcggcg acctggtggt
      601 catgcaggac ggcttcagca gctcgggcct cagcccgaag atggccacgg cgatcgacca
      661 gcttcccgag gtgtccgaga ccgtcgggct cagcaacgtg gtcgccaccg tcgacggcac
      721 gaccgtctac ccgaccgccg gcgacccggc cgccatggcg aacctgctcg acctcgacgt
      781 ccagcagggc gacctggcca agctgcgacc cggccaggtc gccgtcaccg agggctacgc
      841 caccgacgag ggcctcgccg tgggcgacga cctcaccatg gacttcgccg acggcgagca
      901 gaccaccttc gaggtcggtg cggtctacgg ggtgggcgag ctgctcggcg acatcgtgat
      961 ggaccaagcc gactggacgc cccacgagct gcggcccggc aacgtcgccg tgctggtggg
     1021 cctggccgac ggcaccacga tcgagcaggg ccgccaggcg gtcgaggccg tcgccaagga
     1081 gtacggcaac ccgaaggtcg aagaccgcga ccagtacatc gaccgggtcg ccggccaggt
     1141 caaccagctc ctgacgctgg tctacggcct gctggtgctc gccatcctga tcgccctgat
     1201 cggcctggcc aacaccctct ccctgtcgat ccacgagcgg tcccgcgagc tcggcctcct
     1261 ccgggcggtc ggcctcaccc ggggtcggct ccgggccatg gtgcgctggg agtcggtgat
     1321 caccgcggtg gtcgggacgg tggtcggcct cggggtcggc acgttcctcg gctgggggct
     1381 ggtgcgggcg ctgagcgccc aggagggctt cgtgtccttc cagctcccga cggccacgct
     1441 ggtggtggtg ctcgtcttgt ccatcggcgc cggggtcctg gcggcggtcc gcccggccca
     1501 ccgcgccgcc aagctcgacg tgctcgccgc catcgccgag ccgtgacccg gaagcctgcc
     1561 cgagccgatg accgaccgta acgtgagcga cacgatgcct ctcctcctcc gcccctgggg
     1621 cgacgtccgc cggctgtggc gctgcctcgc ctacctcgcg gtcggcgtgc cgctcggcat
     1681 cgtgtcgttc acgacggtct tcaccctcac ggtcttgacg ctctcgctgc tgatcacgct
     1741 ggtgctcgcc atcccggcgg cgtggggact gttcatcgcc gcccggatcc tgggctccat
     1801 cgaccggagc cgggccgcct cgctcctggg catggagctg gtggacccgg tcccgcccct
     1861 ccagggccgc aactggttcc ggcggctgtg ggagcgggtg cgcacccgct cgcgctggcg
     1921 ggagatcgtc aacgccttcc tcctgttccc ggtctcgctg gcctgctggc tgaccacggc
     1981 gacgacgtgg gccgggtcgt tcgccctgct ggcgatgccg ctgtacgccg ggcacctgcc
     2041 gggcgggacg gcgaagttct ggctgttcga ggtcggcccc gggccgggtt cgctggcggc
     2101 ggcggtcgcc ggcctgggag gcctgctggt ggtggccccg tgggtcacga tcggcgccgc
     2161 caacctgcag tcgggcatga cccggttcct cctcggccgg accaccgaag ccgagatggc
     2221 ccggctcctc cagcgggccg aggagcagcg gtcctcggcc gtcgacagcg ccgagaccga
     2281 gcgccggcgc atcgagcggg acctccacga cggcgcccag cagcggctgg tggccctggc
     2341 cgccgagctc ggcgccgccc gcgagaagct cgacgaggac cccgaggccg gcaaggagct
     2401 ggtggccgcc gcccacgagg aggccaaggc ggcgctcaag gacatccgcg acctcgtccg
     2461 gggcatccac ccggtgatcc tcgaggaccg cggcctcgac gccgccctgt cggccgtggt
     2521 cgctcgggca cccgtgccgg tgcacctccg ggtgcgcgtc gagccccggc cggctccggc
     2581 gg
//
LOCUS       DATMFS010000333         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_128131, whole genome shotgun sequence.
ACCESSION   DATMFS010000333 DATMFS010000000
VERSION     DATMFS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128131"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VNQ33_06020"
     CDS             <1..270
                     /locus_tag="VNQ33_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWJ97698.1"
                     /translation="LDGELILCDEVLTPDSSRFWPADQWVPGSTPPSFDKQPVRDYLD
                     GLDWDKTPPPPPLGDDIVAATRARYVEAYERITGNSFKDWPGVQG"
     gene            621..881
                     /gene="purS"
                     /locus_tag="VNQ33_06025"
     CDS             621..881
                     /gene="purS"
                     /locus_tag="VNQ33_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWJ97699.1"
                     /translation="MLFSVQVEVSLREGVADPQGATIERSLPHLGFDGVRGVRVGKSI
                     RFDLEAADQAAAEAEVADLCQRFLTNPVIEDSVITVTQGASA"
     gene            881..1555
                     /gene="purQ"
                     /locus_tag="VNQ33_06030"
     CDS             881..1555
                     /gene="purQ"
                     /locus_tag="VNQ33_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWJ97700.1"
                     /translation="MAPRVGVVQFPGSNCELDVLEAVRLLGGEAEILWHGDATVNGVD
                     AVIVPGGFAHGDYLRPGAIARFSPAMAAVDEFAKSGGPVVGICNGFQVLTEAGLLPGA
                     LQKNRGLKFLCTTVELRVDSTDSVLTSEADLGAVLRVPINHFEGNYTCSPEVLAELQA
                     NEQIVLRYVKNPNGSRDDIAGICNAGRNVVGLMPHPERAVSDLLGSQDGVPLLRSILL
                     QAAAAA"
     gene            complement(1630..2016)
                     /locus_tag="VNQ33_06035"
     CDS             complement(1630..2016)
                     /locus_tag="VNQ33_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97701.1"
                     /translation="MSDEHKAALAEGRSQGRAVRLYLEALEANKPKRGRKRTPESVQK
                     RLDKITSELPTADPLKRLQLTQEQLDLEAELAAGESTVDLSELEKGFIASAAAYASRK
                     GISYGAFRSVGVTPAVLRAAGISRSS"
BASE COUNT          344 a          798 c          778 g          397 t
ORIGIN      
        1 ctcgacggcg agctgatcct ctgcgacgag gtcctcaccc cggactcgtc gcgcttttgg
       61 ccagccgatc agtgggtgcc gggctccacc ccgccttcct tcgacaagca gccggtgcgc
      121 gactacctgg acgggctcga ttgggacaag accccgcccc cgccaccgct cggcgacgac
      181 atcgtggccg ccacccgcgc ccgctacgtc gaggcctacg agcgcatcac cggcaactcc
      241 ttcaaggact ggccaggggt ccagggctga cggcctgctc gaccgcgccc gcgtgcgtcg
      301 gcgccgtcac cgtcggctgg tcgtggctct cggtctgagg aaccacgcgc ccagggcagt
      361 ccgggtgacg tcatcgccgt cgcgcctgcg ctcgccagtc acggatccca cggtgcgtgg
      421 gcggaatcgg acctgttgcg gaacagcgct gatttcgggt cacaagtgtg cgctggaggt
      481 gcacagatga cctgaaatca gcgagcgtgt cgggttgagg ccagctcgag cacgcaacgt
      541 gagaagcgag caaggcgatc acggcctcgt catcccttga ggttcccggc ggcccatcgc
      601 cgtctgcgat gatgaggacc atgctgttct ctgtgcaggt cgaggtctcg ctccgtgagg
      661 gggtggccga cccgcagggt gccaccatcg agcggtccct tccccacctc gggttcgacg
      721 gggtccgggg cgtgcgggtc ggcaagtcga tccgcttcga cctggaggcc gccgatcagg
      781 cggccgccga ggccgaggtc gccgacctct gccagcggtt cttgaccaac ccggtgatcg
      841 aggactccgt catcaccgtg acccaaggcg cctcggcctg atggcgcccc gagtcggcgt
      901 cgtccagttc cccggctcca actgcgagct ggacgtcctc gaggccgtcc gcttgctggg
      961 cggcgaagcc gagatcttgt ggcacggcga tgcgacggtg aacggcgtcg acgcggtcat
     1021 cgtccccggc ggcttcgccc acggcgacta cctccggccc ggggccatcg cccgtttctc
     1081 cccggcgatg gccgcggtgg acgagttcgc caaatccggc gggccggtcg tggggatctg
     1141 caacgggttc caggtgctca ccgaggccgg cctgctcccc ggtgcgctcc agaagaaccg
     1201 gggcctgaag ttcctttgca ccacggtgga actgcgggtc gactccactg attcggtcct
     1261 caccagtgag gccgatctgg gtgctgtcct ccgggttccg atcaaccact tcgagggcaa
     1321 ctacacctgc tcgcccgagg tgctggccga gctgcaggcc aacgagcaga tcgtgctgcg
     1381 gtacgtcaag aaccccaatg gatcgaggga tgacatcgcc ggcatctgca atgccgggcg
     1441 caacgtggtg ggcttgatgc cccaccccga gcgggccgtc agcgacctgc tcggctccca
     1501 agacggcgtg ccgctgctgc gcagcatcct gctccaagcc gctgctgccg cctgagcccg
     1561 gacgcgccac ggcgccggcc ccctcggtgg gagccgacgc cgtgatcagg tcgttggagc
     1621 tcggacggtt caggaagaac ggctgatgcc ggcggcgcgg agcacggccg gggtgacgcc
     1681 gacggagcgg aacgccccgt agctgatgcc cttgcgcgac gcgtaggccg ccgccgaggc
     1741 gatgaagccc ttctccagtt cggagaggtc gaccgtgctc tctccggcag cgagctcggc
     1801 ctcgaggtcg agctgctcct gggtgagctg gaggcgcttc aacggatctg cggtgggcag
     1861 ctccgaggtg atcttgtcga gtcgcttctg cactgattcc ggggtgcgct tgcgaccccg
     1921 cttgggcttg ttggcctcca gagcttcgag gtagagccgt acggctcgtc cctgcgagcg
     1981 gccctccgcc agggctgcct tgtgctcatc ggacatggtt cgggtcttct ttttggcggg
     2041 cattgttcta gagaaccacg aatcgaagcc gaccacaagt cggcaccatg atatgtccga
     2101 atcggcgcca cctcatttca ccatttcgat tggctgacag caccatcaca cgtcctcatt
     2161 cgcgccagga aggagtacct ctggtgaggc gaacacggca ccgtcgtgcg gtgccgtcga
     2221 ggccgccctc ccgcagccgc cggatgcacc tccggcggtt tgcgcaccgt gctcgcgcct
     2281 gacgccaagc ccggaccggt ctcctgacgg aaggtgg
//
LOCUS       DATMFS010000334         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_35440, whole genome shotgun sequence.
ACCESSION   DATMFS010000334 DATMFS010000000
VERSION     DATMFS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35440"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VNQ33_06040"
     CDS             <1..1900
                     /locus_tag="VNQ33_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97702.1"
                     /translation="TRLFCAALALSALAVTAPILTPPASAYATYQTTIIARATDSNGA
                     QYPGGTTAARLSADGRWLAFSRPGTGGSYRKSLISGETTLISLNRAGQPANAASTIEG
                     ISADGSIVLFSTTATNLGLGPATARDLYVRNISDGVTDRANLTGNDGSQVAVKAGEAS
                     LSDDGSYVGFATTTNHVWVRSPSSHYSEQVDVLDKGQVPNGSSGQPSLSTDGRYVTFT
                     SYASNLVVNDTNFVPDVFQLDRQSHLIIRVSLSDTEVAPNSGSTEPSVSGDGRYVAFT
                     SDATNIVTGDTNNTKDVFVRDIIGGTTRRISVRTGGAQANGESTHPDIDAAGGSVAFE
                     STADDLDLSVSKNAKTDVYRRELSGNLTKQIGNDGYTNTDQGATGASLASNGSVAFTS
                     ASTNLLQGDTNGAADAFVQGYQPLGPFAGFGPMASMLHADFGISAAKNATVQADLQNG
                     RVTPGHLIVRSAHDPVWAQHREPVARLYQAFFHRVPDLNGLNYWVKKRRGGTKLSVIA
                     ASFAGSNEFKTAYGKVDSSTFVTLVYGNVLQRKPDAPGLAHWVAKMNSGMSRGDVMVA
                     FSESNEGKRFLAPEVDATLMGLGMLHAMPPKALWKQVADASRDHHMSEWGALTYLNSA
                     EFVQLVNT"
     gene            2012..2413
                     /locus_tag="VNQ33_06045"
     CDS             2012..2413
                     /locus_tag="VNQ33_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWJ97703.1"
                     /translation="MLAADVGSGDVLVTLLWFTMLVIWVYLLIIVATDLFRSRDLSGV
                     AKFLWLLFIVVLPYVGIFAYLIVRGHEMHDHAVADAQAADHAAQAYIRQAAAGAGVGI
                     AGELERLADLRDSGAITGAEFQKRKAQLLER"
     gene            complement(2546..3127)
                     /locus_tag="VNQ33_06050"
     CDS             complement(2546..3127)
                     /locus_tag="VNQ33_06050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ97704.1"
                     /translation="MIVLVRHGATAWSTARRHTGRTDVPLTDEGRAQASALAPVLAGL
                     RPDRVLTSPLSRATETCALAGFGDQAEADDRLLEWDYGDAEGRTTAEIREDLDPLWSV
                     WTHPIAGAEPLASVAARVDGVIADLRAGDGDPVVLVFAHAHLLRILGARWCGLDAVAG
                     QSLVLDPASVSVLGNERETPVIERWNVVADPFA"
     gene            complement(3171..3890)
                     /locus_tag="VNQ33_06055"
     CDS             complement(3171..3890)
                     /locus_tag="VNQ33_06055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWJ97705.1"
                     /translation="MIEREITVTTPEGELPTFLFHPEEGGPHPVVLYLMDAPGIRPAL
                     REMAARLASSGYYVMLPYLYYRGGPFQELGMSDEDAHKRRELMGQITPTNIVSDAEAL
                     LAAAAEDPAAGDGPVGVIGFCMSGGMAISVARAMPGRVAAAASIHGAWLVRDGDDSPH
                     LGLDSVAAEIHLAWCDPDPTAPTEQREQLVAALEEAGITYTLDVETDAQHGFAPPGPR
                     HHPEASERHWDRVLDLLDRNL"
     gene            complement(3959..>5163)
                     /locus_tag="VNQ33_06060"
     CDS             complement(3959..>5163)
                     /locus_tag="VNQ33_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HWJ97706.1"
                     /translation="TFAGLVGVNRLFPTKALSNADSTFVVAPNHDTLYVIAVLDVAFA
                     PQLLTLPDIDDRYHVIQILDAWMGGLALLGTRETGGRAGRWAIALDGADVDVPDGVTR
                     IDSPTSHVFVLGRIRATEDPDDVAAAADVARSITMLPLTEPDPSYRGPGATLGEPFGA
                     PQEVGTNGAAFFDEVGSLLGTDGPVTPAQTAAFAAAGGLVGAGRYPTTERPDRVPELE
                     KAVDEGLAGLAEGFAGSIDLVNGWAVNLDLGQADDRMSLRDQAVIARYFWGPVPAEEA
                     VYPRAVEASDGKALDGSKTYRIRFDGDDLPPVDAFWSLTVYGEDLYFAPNEIDRYSLS
                     GDTPGLVAGDDGSIEVVLAHDRPEGGDANWLPTPDGPFTLIMRLYLPQQPILDGTWDY
                     PSIELVDR"
BASE COUNT          778 a         1921 c         1764 g          700 t
ORIGIN      
        1 aacccgtctc ttctgcgctg cgctggccct ctccgcgctc gccgtcaccg ccccgatcct
       61 caccccgccg gcctcggcct acgccaccta ccagacgacc atcatcgccc gagccaccga
      121 ctcgaacggg gcccagtacc ccggcgggac caccgccgcc cgcctctcgg ccgacggccg
      181 gtggctggcg ttcagccgcc ccggcaccgg cggcagctac cgcaagagcc tcatcagcgg
      241 cgagaccaca ctcatctcgc tgaaccgtgc cggtcagccg gccaacgccg cctccacgat
      301 cgagggcatc agcgccgacg gcagcatcgt cttgttcagc accacggcca ccaacctggg
      361 cctcgggccc gcgaccgccc gggacctcta cgtccgcaac atcagcgatg gcgtgaccga
      421 tcgagccaac ctgacgggga acgacggcag ccaggtcgcc gtcaaggccg gagaggcgtc
      481 gctgagcgac gacgggtcct acgtcggctt cgccaccacc acgaaccacg tgtgggtgcg
      541 ctccccctcg agccactact ccgaacaggt cgacgtgctg gacaagggcc aggtgcccaa
      601 cggcagctcc ggccagccga gcctgagcac cgacggccgc tacgtcacgt tcaccagcta
      661 cgccagcaac ctcgtggtca acgacaccaa cttcgtccct gacgtgttcc agctggaccg
      721 ccagtcgcat ctgatcatcc gggtcagcct cagcgacacc gaggtcgccc ccaacagcgg
      781 gagcaccgag ccgtcggtca gcggcgacgg tcggtacgtg gcgttcacct ccgacgcgac
      841 caacatcgtc accggcgaca ccaacaacac caaggacgtg ttcgtccggg acatcatcgg
      901 cgggaccacc cggcgcatca gcgtgaggac cggcggtgcc caggccaacg gcgagtcgac
      961 ccaccccgac atcgacgcgg ccggcggctc cgtcgccttc gagtccacgg ccgacgacct
     1021 cgacctctcc gtgtcgaaga acgccaagac cgacgtctac cgccgcgagc tgtctggcaa
     1081 cctgaccaag cagatcggca acgatgggta caccaacacc gaccaggggg cgacgggcgc
     1141 gtcgctcgcg tcgaacggat ccgtcgcctt cacctcggcc agcaccaacc tgctgcaagg
     1201 cgacaccaac ggggccgccg atgcgttcgt ccaggggtac cagccccttg ggcccttcgc
     1261 cgggttcggg cccatggcct cgatgctcca cgccgacttc ggcatcagcg cggcgaagaa
     1321 cgccacggtg caggccgacc tccagaacgg gcgggtcacc cccggccacc tcatcgtccg
     1381 ctccgcccac gaccccgttt gggcccagca ccgcgagccc gtcgcccgcc tctaccaggc
     1441 cttcttccac cgggtgccgg acctgaacgg actgaactac tgggtgaaga agcgtcgagg
     1501 cggcaccaag ctcagcgtca tcgccgcctc cttcgcgggg tcgaacgagt tcaagacggc
     1561 gtacggcaag gtcgacagca gcaccttcgt caccctcgtc tacggcaacg tcctccagcg
     1621 caagcccgac gcccccggcc tcgctcactg ggtggccaag atgaacagcg gcatgagccg
     1681 cggcgacgtg atggtggcct tctcggagag caacgaaggg aagcggttcc tcgcccccga
     1741 ggtcgacgcc accctgatgg gcctcgggat gctccacgcc atgccgccca aggcgctctg
     1801 gaagcaggtc gccgacgcga gccgggacca ccacatgtcg gagtggggtg cgctcaccta
     1861 cctgaacagc gccgagttcg tccagctcgt caacacgtag ccggcgtcgt cctgcccgcc
     1921 ctgcccgaat cccccgggca ggggagggca ggcgggtcta ccgtcgagac cgcgaccccc
     1981 gagggcgggg tcccctccga aaggcgcacc catgctcgct gctgatgtcg gatccggtga
     2041 cgtcctcgtg accctgctgt ggttcacgat gctggtcatc tgggtgtacc tgctgatcat
     2101 cgtggccacg gacctgttcc gcagccgcga tctcagcggt gtcgccaagt tcctctggct
     2161 gctcttcatc gtcgtgttgc cctacgtcgg gatcttcgcc tacctgatcg tccgcgggca
     2221 cgagatgcac gaccacgccg tggccgatgc ccaggccgct gaccacgctg cccaggccta
     2281 catccgccag gccgccgccg gtgccggcgt cggcatcgcc ggcgagctcg agcgcctggc
     2341 cgacctccgc gacagcggcg ccatcaccgg cgccgagttc cagaagcgca aggcccagct
     2401 cctcgagcgc tgaccgcctc ctggaccgac gactcgacag tccagagggc gtctacgcct
     2461 acgccctcga cccgtcgacc gccgcggcac ggtgggccgc tccgaggacc tgggcggcga
     2521 gtcctccccg gactgacgac cctggtcagg cgaaggggtc ggccacgacg ttccagcgct
     2581 cgatgacggg cgtctcccgc tcgttgccga ggacgctgac gctcgccggg tccagcacga
     2641 ggctctgacc ggcgacggcg tcgagcccgc accaccgggc accgaggatg cgcagcaggt
     2701 gggcgtgggc gaagacgagg accacgggat cgccgtcgcc ggcacggagg tcggcgatca
     2761 cgccgtcgac gcgcgccgcc accgacgcca gcggctcggc gccggcgatc gggtgggtcc
     2821 acaccgacca cagggggtcg aggtcctcgc ggatctccgc cgtggtccgg ccctcggcat
     2881 cgccgtagtc ccactcgagg aggcggtcgt cggcctcggc ctggtcgccg aacccggcca
     2941 gcgcgcaggt ctccgtcgcc cgcgacagcg ggctggtcag cacccgatcg ggtcggaggc
     3001 cggcgaggac cggcgccagc gccgaggcct gggcgcgccc ttcgtcggtg agcgggacgt
     3061 cggtgcgtcc ggtgtggcgc cgcgcggtgc tccacgccgt ggcgccgtgg cggacgagga
     3121 cgatcatccc gtcaccctcc cacgcccggc ggacgggtca cccactcccg tcagaggttg
     3181 cggtcgagga ggtcgaggac ccggtcccag tggcgctcgg aggcctccgg gtggtggcgg
     3241 ggtccgggcg gggcgaagcc gtgctgggcg tcggtctcca cgtcgagcgt gtaggtgatg
     3301 ccggcctctt cgagggcggc gaccagctgc tcgcgctgct ccgtcggcgc cgtcggatcc
     3361 ggatcgcacc aggcgaggtg gatctcggcc gccaccgagt ccagcccgag gtgcggcgag
     3421 tcgtcgccgt cgcgcaccag ccaggcgccg tggatggagg ccgcggcggc gacgcggccg
     3481 ggcatcgccc gggccacgga gatcgccatg ccgccgctca tgcagaagcc gatgaccccc
     3541 accgggccgt cacccgctgc agggtcctcc gccgcggcgg cgaggagggc ctcggcatcg
     3601 ctgacgatgt tggtgggcgt gatctggccc atcagctccc ggcgcttgtg ggcgtcctcg
     3661 tcgctcatgc ccagctcctg gaacggaccg ccccggtagt agaggtacgg cagcatcacg
     3721 tagtacccgg agctggccag gcgagccgcc atctcccgca gcgccggccg gatgcccggc
     3781 gcgtccatca ggtagaggac gaccgggtgc ggcccgccct cctccgggtg gaacaggaac
     3841 gtgggcagct caccctccgg ggtggtgacg gtgatctcgc gctcgatcat gtgaggagtc
     3901 tcccagcccg cggctcgatt gccacccatc gggaccagcc gccgagcctg cccccgggtc
     3961 agcggtcgac gagctcgatc gaggggtagt cccacgtgcc gtcgaggatc ggctgctgcg
     4021 ggaggtacag ccgcatgatc agcgtgaacg ggccatcggg ggtcggcagc cagttggcgt
     4081 cgccgccctc gggccggtcg tgggcgagga cgacctcgat cgagccgtcg tcgccggcca
     4141 ccagccccgg cgtgtcgccc gagagcgagt accggtcgat ctcgttggga gcgaagtaca
     4201 ggtcctcgcc gtagacggtg agcgaccaga aggcgtcgac cggcgggagg tcgtcgccgt
     4261 cgaaacggat ccggtaggtc ttggatccgt cgagcgcctt gccgtcggac gcctcgacgg
     4321 ctcgcgggta gaccgcctcc tcggccggca ccgggcccca gaagtaccgg gcgatcacgg
     4381 cctggtcgcg gaggctcatc cggtcgtcgg cctggccgag gtccaggttc accgcccacc
     4441 cgttcaccag gtcgatcgag ccggcgaacc cctcggccag accggccagg ccctcgtcga
     4501 cggccttctc cagctcgggc acccgatcgg ggcgttcggt cgtcgggtac ctcccggcgc
     4561 ccaccagccc gccggccgcg gcgaacgccg cggtctgcgc cggggtgacc ggaccgtcgg
     4621 tgccgaggag cgagccgacc tcgtcgaaga aggcagcgcc gttggtgccc acctcctgcg
     4681 gcgccccgaa cggctcgccg agggttgcgc ccgggccccg gtacgagggg tcgggctcgg
     4741 tgagcggcag catcgtgatc gaacgggcga cgtcggccgc ggcggcgacg tcgtcggggt
     4801 cctcggtggc ccggatccgc ccgaggacga acacgtgcga cgtcgggctg tcgatgcggg
     4861 tcacgccgtc gggcacgtcg acgtcggcac cgtccagcgc gatggcccag cggcccgctc
     4921 ggccaccggt ctcccgcgtc ccgaggaggg ccagcccgcc catccacgca tcgaggatct
     4981 ggatcacgtg gtagcggtcg tcgatgtcgg ggagcgtgag gagctggggc gcgaacgcca
     5041 cgtcgaggac ggcgatcacg tagagcgtgt cgtggttggg cgccacgacg aacgtcgagt
     5101 cggcgttcga cagcgccttc gtggggaaca ggcggttcac gcccacgagc ccggcgaacg
     5161 tct
//
LOCUS       DATMFS010000335         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_81459, whole genome shotgun sequence.
ACCESSION   DATMFS010000335 DATMFS010000000
VERSION     DATMFS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81459"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VNQ33_06065"
     CDS             <1..1317
                     /locus_tag="VNQ33_06065"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97707.1"
                     /translation="GYSGPDSFTFTASDGSLSDSGTISISVGTPTNTAPTATDQSVAV
                     PHNTATPITLSGTDPEGDALTFAATSTPAHGALSGTAPNLTYTPAAGYSGPDSFTFTA
                     SDGSLSDSGTISLTVAAAPVTVPGAPAIGSASAAEGEATISWTAPASNGGSAITGYKV
                     TPYANGTAKPAIDVAAGTTSLLVTGLANGTPHTFKVAAVNAVGTGPDSAASNAVTPQW
                     WLPWSSGSAAVTEIYTWMTSKAPTSAQKASWLNQMNAGTKLPADLVVALRGAADATTN
                     VDPTVRLYSAYLTRVPDAGGLNYWLKRRRAGWTLSKISSNFAGSSEFIRRYGSMTNRK
                     FVENIYVNVLRRTGDPAGMDYWTKKLDAKKASRGQVMINFSESNEYKNKQVNNTHAAV
                     IFIHLLGKTPTAVQRDAFVASLVGGTSLRDLVRDQIHQPSFAARAG"
     gene            complement(1457..1972)
                     /locus_tag="VNQ33_06070"
     CDS             complement(1457..1972)
                     /locus_tag="VNQ33_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97708.1"
                     /translation="MPTDDDRSSAPAGWLLVADHRVLDVDPMIEAVGQVYRYPVADAE
                     RAATMEPGQPCFLLRTDASRVIGLWAVGEVVGESAPLAEGTDGAGDGEPRWAAEVELL
                     PLEKAIARSKLLDHTMLAAGALADASFADTPIPLLRRDVRALETFDFFLVEPSPDQRD
                     ALDALLDAEET"
     gene            2040..>2578
                     /locus_tag="VNQ33_06075"
     CDS             2040..>2578
                     /locus_tag="VNQ33_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWJ97709.1"
                     /translation="MDTFALVADERRSLAGVLESLSADDWTQPSLCGAWSTHQVAAHL
                     NVPFAVGSLTFVVEIAKALGRFDKANERVAVDLATRLSPAECIAGLRANADSRFTPPM
                     FGPEAPLTDTIVHGGDILQPVGRTVAVAPEALSVSLPFITSAKARRGFGAVSADGLRI
                     APTDLDLTIGDGEELAGPAR"
BASE COUNT          388 a          963 c          859 g          368 t
ORIGIN      
        1 gggtactcgg gtccggactc gttcacgttc acggcgtcgg acggctcgct gtcggactcc
       61 ggcactatct cgatcagcgt cggcacgccg acgaacaccg ccccgacggc cactgatcaa
      121 tcggtcgccg tcccccacaa cacggcgacg ccgatcacgt tgagcgggac cgatcctgag
      181 ggtgatgcgt tgacgttcgc ggcgaccagc acgccggcgc acggcgcgct gtcgggtacg
      241 gcgccgaacc tgacctacac gccggctgcc gggtactcgg gcccggactc gttcacgttc
      301 acggcgtcgg acggctcgct gtcggactcc ggcaccatct cgctgaccgt ggccgccgcc
      361 ccggtcaccg tccccggcgc accggccatc ggatcggcct ccgccgccga gggcgaggcg
      421 accatcagct ggaccgcacc ggccagcaac ggcggctccg ccatcaccgg ctacaaggtc
      481 acgccgtacg ccaacggcac ggccaagccg gcgatcgacg tcgctgccgg aaccacgtcg
      541 ctgctcgtga ccggtctggc caacggcacg ccccacacct tcaaggtggc ggcggtgaac
      601 gccgtcggca ccggccccga ttcggctgcc tccaacgcgg tgacgccgca gtggtggctg
      661 ccctggtcgt cgggatcggc cgcggtgacc gagatctaca cctggatgac gagcaaggcc
      721 ccgacctcgg cccagaaggc ctcgtggctc aaccagatga acgcggggac caagctcccc
      781 gccgacttgg tggtggccct gcgcggcgcg gccgacgcca ccaccaacgt cgacccgacg
      841 gtgcggctct actccgccta cctgacccgg gtacccgacg ctggcggcct caactactgg
      901 ctcaagcggc gccgggccgg atggacgctc tcgaagatct ccagcaactt cgccgggtcc
      961 tccgagttca tccgccgcta cggctcgatg accaaccgca agttcgtgga gaacatctac
     1021 gtcaacgtcc tccgccggac cggcgacccg gccggcatgg actactggac caagaagctc
     1081 gacgccaaga aggcgtcgcg cggccaggtg atgatcaact tctccgagtc gaacgagtac
     1141 aagaacaagc aggtcaacaa cacccacgcc gcggtcatct tcatccacct gctgggcaag
     1201 acgccgaccg ccgtgcagcg cgatgcgttc gtcgcctccc tggtgggcgg gacctcgctg
     1261 cgggacctcg tgcgagacca gatccaccag ccgtcgttcg ccgcccgcgc cggctgaccc
     1321 cccggaccga acccgatttc gggtcacctg ggcaccactg tggtgctcag gtgacccgaa
     1381 atcgcgtcgt gggacccatt cgggtcacgc gtgcaccgat gtggtgctca ggtgacccga
     1441 aatcgggttg tggccatcag gtctcttcgg cgtccaacag ggcgtcgagg gcgtcgcgct
     1501 gatcggggct cggctcgacc aggaagaagt cgaacgtctc cagcgctcgc acgtcgcggc
     1561 gcaggagcgg gatcggggtg tccgcgaacg aggcgtcggc cagcgcgccg gccgcgagca
     1621 tcgtgtggtc gaggagcttg gagcgggcga tggccttctc cagcgggagc agctcgacct
     1681 cggcggccca gcgcggctcg ccgtcgccag ccccgtcggt gccctcggcg agcggcgccg
     1741 actcgccgac cacctcgccc accgcccaca ggccgatcac cctcgacgca tcggtccgga
     1801 gcaggaagca cggctggccc ggttccatcg tcgccgcacg ctcggcgtcg gccaccgggt
     1861 agcggtacac ctggcccacg gcctcgatca tcgggtcgac gtcgaggacc cggtggtcgg
     1921 ccaccaggag ccagccggcg ggggcggagg agcggtcgtc gtcggtgggc atcgtcgcca
     1981 tgctcgcaga cggccgggcc cgccagcgcc gtcggtccag tgtcgtagcg tcacggccca
     2041 tggacacgtt cgccctcgtc gccgatgagc gccgttccct cgccggcgtc ctcgaatcgc
     2101 tctcggccga tgactggacc cagccgtcgc tctgcggtgc ctggtccacg caccaggtgg
     2161 cggcgcacct gaacgtgccg ttcgccgtgg gctcgctcac cttcgtggtc gagatcgcca
     2221 aggcgctcgg ccgcttcgac aaggccaacg agcgcgtggc ggtcgacctg gcgacgaggc
     2281 tcagcccggc cgagtgcatc gccgggctgc gggcgaacgc cgactcacgg ttcacgccgc
     2341 cgatgttcgg tcccgaggcg ccgctcaccg acacgatcgt ccacggcggc gacatcctcc
     2401 agccggtggg caggaccgtg gccgtcgcgc ccgaggcgtt gtccgtctcg ctgccgttca
     2461 tcacgtcggc caaggcccgc cgcggcttcg gtgccgtgag cgccgacggc ctccggatcg
     2521 ctccgaccga cctcgacttg accatcggcg acggcgagga gctggccggt cccgcccg
//
LOCUS       DATMFS010000336         3155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_35483, whole genome shotgun sequence.
ACCESSION   DATMFS010000336 DATMFS010000000
VERSION     DATMFS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35483"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VNQ33_06080"
     CDS             complement(<1..562)
                     /locus_tag="VNQ33_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97710.1"
                     /translation="MQDLGSGKTAERPVRLGGALVTIVEPHKGHEVAYNRWYERDHFY
                     AGCMIGAWNISGQRFVATKDLKDLRYPADSPVIPDPATGSFLALYWVLAGKFGQWMQW
                     GSEQVKWLHENDRMFTERDHVHTLMYKFRTEFEHPDGVPVELALEHHSPYVVVVIGEP
                     DEGKDLDDVDAWFRSRPLPGVVGAELT"
     gene            704..1546
                     /locus_tag="VNQ33_06085"
     CDS             704..1546
                     /locus_tag="VNQ33_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWJ97711.1"
                     /translation="MTATTFEIETLGNVAHLRLNRPDAYNSMTREFWSELPGAVRELD
                     ASGSVRALVISSTGKHFCAGMDLGVFTGGAPGNEGGSTEIGRVRAQLRETVLALQGTF
                     TALEEARFPVIAAIQGGCIGGAVDMVSACDLRYATADAFFCVQEINLGMTADVGTLQR
                     LPKIIPDGIAREWAYRGHRVPATRAAEVGLVNEVFDTQEALLDGVLAIAQEIAGKSPL
                     AIWGTKEMATYTRDHSVADSLKHMAAWQSGMFQPADMMETFVAKGEGREPVFDDLLPH
                     GTGL"
     gene            1554..1919
                     /locus_tag="VNQ33_06090"
     CDS             1554..1919
                     /locus_tag="VNQ33_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97712.1"
                     /translation="MGIVRLDHVQLAMPAGGEDDAAAFYEGVLGIPQVPKPPHLAARG
                     GCWFERGALKVHLGVDADFRPATKAHPAFEVDDVRSLAAAIGAAGYQVTEDEPLEGYD
                     RVYALDPFGNRLELLQPLG"
     gene            complement(1955..>3155)
                     /locus_tag="VNQ33_06095"
     CDS             complement(1955..>3155)
                     /locus_tag="VNQ33_06095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWJ97713.1"
                     /translation="ATDRGVARALSIRPAVWIGDVSYGWYLWHWPLIVFAAVLWPGRD
                     GVLVVVALLSLIPTALSYRFVENPIRFAPGAASRRVWPLASVCILVPVAAFAGVLVIG
                     RAQAKSATVERFSHEIREHEDRRRGCDAPEPIGEREAQDCTWTVDGAKGAVFLVGDSN
                     AGHFSEPMITAAKAAGYDLTVATSAGCPFADVIRRAPAVGFDGDVCRASVQDSVDALA
                     ELHPSLVVIAASSTEWVQTSTNQLVDPETGERGRTPEEKAEIYEAGLRRVLEELDEAG
                     VPTAVVHTVPQFNATLGGTWKAETCAAIQIYLDRCGGSRSLEGVEREQAPSVAAEDAA
                     AAGLGTATTVGLDDLLCTDGRCRTSRGDLRLYRDAAHLTVDGAELLAPRFTEVVEQHA
                     VPRAPGG"
BASE COUNT          456 a         1183 c         1091 g          425 t
ORIGIN      
        1 cggtgagctc ggcacccacc acgccgggca gcgggcggga gcggaaccag gcgtcgacgt
       61 cgtcgaggtc cttgccctcg tccggctcgc cgatgaccac caccacgtag ggcgagtggt
      121 gctccagggc cagctccacg ggcaccccgt cggggtgctc gaactcggtc cggaacttgt
      181 acatgagggt gtggacgtgg tcgcgctcgg tgaacatgcg gtcgttctcg tggagccact
      241 tcacctgctc gctgccccac tgcatccact ggccgaactt gccggcgagc acccagtaga
      301 gggccaggaa cgacccggtc gccgggtcgg ggatcaccgg cgagtcggcc gggtagcgca
      361 ggtccttcag gtccttggtg gcgacgaagc gctggccgct gatgttccag gcgccgatca
      421 tgcagccggc gtagaagtgg tcgcgctcgt accagcggtt gtaggccacc tcgtggccct
      481 tgtggggctc gacgatggtg accagggcgc cgcccagccg gaccgggcgc tccgccgtct
      541 tgccgcttcc caagtcctgc acgagggtcc cctgctggtg tcgaggtgtc cgggcggtcc
      601 cggaccgaaa acgagaacgt gttctcgttt tcagcatgcc acgccgcacg gcgggctgcg
      661 ccatcaagag gtcggcggtg gcccgaggtg cgatcatgac cgcatgaccg ccaccacgtt
      721 cgagatcgag accctcggga acgtcgccca cctccggctc aaccggcccg acgcctacaa
      781 ctccatgacc cgcgagttct ggtccgagct gcccggcgcg gtgcgggagc tcgacgccag
      841 cgggtcggtg cgggccctcg tgatctcctc caccggcaag cacttctgcg ccggcatgga
      901 cctcggcgtg ttcaccggcg gggcccccgg caacgagggc ggctccaccg agatcggccg
      961 ggtgcgggcc cagctgcgag agaccgtgct cgccctccag ggcacgttca ccgccctcga
     1021 ggaggcccgc ttccccgtga tcgccgccat ccagggcggc tgcatcggcg gcgccgtcga
     1081 catggtgtcg gcgtgcgacc tgcggtacgc caccgccgac gccttcttct gcgtccagga
     1141 gatcaacctc ggcatgaccg ccgacgtcgg cacgctccag cgcctgccga agatcatccc
     1201 cgacggcatc gcccgcgagt gggcctaccg cggccaccgc gtgccggcga cccgggccgc
     1261 cgaggtcggg ctggtcaacg aggtgttcga cacccaggag gccctgctcg acggcgtgct
     1321 ggccatcgcc caggagatcg ccggcaagag ccccctcgcc atctggggca ccaaggagat
     1381 ggccacctac acccgcgacc actccgtggc cgacagcctc aagcacatgg cggcgtggca
     1441 gtccggcatg ttccagccgg ccgacatgat ggagacgttc gtcgccaagg gcgaaggccg
     1501 cgagccggtg ttcgacgacc tcctccccca cggcaccggg ctctgagcgc gccgtgggca
     1561 tcgtccgact ggaccacgtg cagctggcga tgccggcggg cggcgaggac gatgcggccg
     1621 cgttctacga gggcgtcctg ggcatcccgc aggtccccaa accgccacac ctggcggcgc
     1681 ggggcgggtg ctggttcgag cgcggcgccc tcaaggtcca cctcggcgtc gacgccgact
     1741 tccgcccggc caccaaggcc caccccgcgt tcgaggtcga cgacgtgcgg tcgctggccg
     1801 cggcgatcgg tgcggcgggc taccaggtca ccgaggacga accgctcgag ggctacgacc
     1861 gcgtgtacgc cctcgacccg ttcggcaacc gcctcgagct gctccagccg ctgggctgac
     1921 gatcgccccc cccccggcgc tggcggcgat cccgtcaccc acccggcgcg cgcggcaccg
     1981 cgtgctgctc caccacctcg gtgaagcgcg gcgccagcag ctcggccccg tcgacggtga
     2041 ggtgggcggc gtcgcggtag agccgcaggt cgccccggct cgtgcggcag cgcccgtcgg
     2101 tgcacagcag gtcgtcgagc ccgaccgtgg tggccgtacc gagccccgcg gccgcggcgt
     2161 cctcggccgc caccgacggc gcctgctcgc gctcgacgcc ttcgagcgac cgcgacccgc
     2221 cgcagcggtc gaggtagatc tggatcgccg cacacgtctc cgccttccag gtgccgccca
     2281 gcgtggcgtt gaactggggg acggtgtgca ccaccgccgt gggcacgcct gcctcgtcga
     2341 gctcctcgag gacccggcgg aggccagcct cgtagatctc ggccttctcc tcgggcgtgc
     2401 gaccccgctc gcccgtctcg gggtcgacca actggttggt ggaggtctgg acccactcgg
     2461 tcgacgaggc ggcgatgacg accagcgagg ggtgcagctc cgcgagcgcg tcgaccgaat
     2521 cctgcacgga cgctcggcac acgtcaccgt cgaagccgac cgcgggagcc cgccggatca
     2581 cgtcggcgaa cggacatccc gccgaggtgg cgacggtgag gtcgtacccc gccgccttcg
     2641 ccgccgtgat catgggctcg gagaagtggc cggcgttgga atcgcccacc aggaacaccg
     2701 cacccttcgc gccgtcgacc gtccacgtgc agtcctgagc ttcccgctcc ccgatcggct
     2761 cgggcgcgtc gcagccccgg cgccggtctt cgtgctcgcg gatctcgtgc gagaagcgct
     2821 ccacggtcgc cgacttcgcc tgggcccgcc cgatcacgag cacgcccgcg aaggcggcga
     2881 cgggcaccag gatgcacacc gacgccagcg gccagacccg gcggctcgcc gcaccgggag
     2941 cgaagcggat ggggttctcc acgaaccggt acgacagcgc cgtggggatc agcgagagca
     3001 gggcgacgac gaccagcacc ccgtcccgcc cgggccagag gaccgcagcg aacacgatca
     3061 acggccagtg ccacaggtac cacccgtagg acacgtcgcc gatccacacc gccgggcgga
     3121 tgctgagcgc ccgcgccacg ccacggtcgg tggcc
//
LOCUS       DATMFS010000337         3266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_220875, whole genome shotgun sequence.
ACCESSION   DATMFS010000337 DATMFS010000000
VERSION     DATMFS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220875"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VNQ33_06100"
     CDS             <1..266
                     /locus_tag="VNQ33_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97714.1"
                     /translation="PRTVAVTLLAVSLVTAAASCAKSDGSDPESTGPVNQAVERLFDF
                     GLTQQQASCVVDEVGADTVVEAADLNAFTESQQYRDAAKACIE"
     gene            263..538
                     /locus_tag="VNQ33_06105"
     CDS             263..538
                     /locus_tag="VNQ33_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97715.1"
                     /translation="MTPPRRLAAFGLVVALAASAAACTSDPKDPAQVREERVQARIAS
                     TFSRSQATCIMKVLDAPTIAALDKTTALPAGSEALRIYSNAVVACTA"
     gene            613..687
                     /locus_tag="VNQ33_06110"
     tRNA            613..687
                     /locus_tag="VNQ33_06110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Cys,seq:gca)
     gene            827..1027
                     /locus_tag="VNQ33_06115"
     CDS             827..1027
                     /locus_tag="VNQ33_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWJ97716.1"
                     /translation="MATGTVKFFNSEKGYGFISREDGDDVFVHFSNIQGTGYKTLDEG
                     QHVEFDVAPGRKGEEAQNVRVV"
     gene            1125..2009
                     /locus_tag="VNQ33_06120"
     CDS             1125..2009
                     /locus_tag="VNQ33_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWJ97717.1"
                     /translation="MSNPALNPKIFQKETAANQQPSAFEPGWGSPADELPPNVFDAQA
                     GVAGAAPTRPPVSAGTGDTMRISGTMTATAILLLLLMVAGYFGWQALDLHQTGVTPAG
                     EKVYAVNPPAWIFAVWIGTFILAMVTIFKPRLARVTAPIYAIGEGLVLGSVSAIFEAS
                     YPGIVVQAVLLTFGVFITMMVLFATGTIRVTEKLRMCIFASMGAILLVYLADIIASLF
                     GSSLPIVNSSSPWGIGFSVIVVIVASLNLLLNFDFIKQAVDHQAPRYMEWFAAFGLML
                     ALVWLYLEILRLLAKLRN"
     gene            2092..3033
                     /locus_tag="VNQ33_06125"
     CDS             2092..3033
                     /locus_tag="VNQ33_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWJ97718.1"
                     /translation="MSDTIACSSCGTEVPVAASFCTGCGQPMASAAGPPPPPPRALEP
                     VPTDAPDVTQVVTPGLHDATQVTPPPPSTPAPPPYAPPAPPPPTAPASGPWGPSSAEP
                     PTAAPWTPPTAAAPGAPTAPPPPTWQQPTPPPSPGTYPPVGGVAAGSWQNPAAPGAAP
                     WGAAPEAAPAAAGGSVLGGVVGLIGAVLALVGIFSGWVKLGNTGTVTAWSLTEGEGLL
                     KTQTPYVILAFAAAAVIVSLVLFTGIARPVARILAVLLGLAIIGVTAANWNEIASFVT
                     DNFPSSFKASTAVGFYLTIAGGVLIIVSALLPAKKKP"
     gene            3030..>3266
                     /locus_tag="VNQ33_06130"
     CDS             3030..>3266
                     /locus_tag="VNQ33_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ97719.1"
                     /translation="MSETHALAGTGALVTGGGSGIGLAIATRLASDGATVTICGRSAE
                     RLDAAVAANGLADGPGSLHAVVADVTDEDAVATAV"
BASE COUNT          448 a         1277 c         1053 g          488 t
ORIGIN      
        1 cccctcgtac cgtggccgtg acgctgctcg cggtgtcgct cgtgaccgcg gccgcgtcgt
       61 gcgccaagag cgatggttcc gaccccgagt ccaccgggcc ggtcaaccaa gccgtcgagc
      121 ggctgttcga cttcggcctc acgcagcagc aggcgagctg cgtcgtcgac gaggtcggcg
      181 ccgacaccgt ggtcgaggcg gccgacctca acgccttcac cgagagccag cagtaccgcg
      241 acgccgccaa ggcgtgcatc gaatgacgcc gccccgccgg ctcgccgcgt tcgggctggt
      301 cgtggcgctc gccgcatccg ccgccgcctg cacctcggat ccgaaggacc cggcccaggt
      361 gcgcgaggag cgcgtgcagg cccgcatcgc gtcgaccttc tcccgctccc aggccacctg
      421 catcatgaag gtgctcgacg cgcccaccat cgccgcgctc gacaagacca ccgccctccc
      481 cgccggcagc gaggcgctgc ggatctactc gaacgccgtg gtggcctgca ccgcctgatc
      541 gcccggaacg ccaggcactc cgccgagggc cttccaggcc gccgagccgg ctccgctagg
      601 gtggctcggc ctggtggcgt ggccgagtgg ctgaggcaac ggcctgcaaa gccgtccacg
      661 cgggttcgat tcccgccgcc acctccaaga tgtccggccg aggtgctctc gcacctcggc
      721 ccggcgtcgt cgcgcgagac ccctttcacg gctcggtttt ctgtcctacc ctgtttccga
      781 actcgtcgct cggacgagtt cgcgcaagca cagagagaga aataccttgg ctacaggcac
      841 cgtgaagttc ttcaactccg agaagggcta cggcttcatc tcccgcgagg acggcgacga
      901 tgtgttcgtc cacttctcca acatccaggg gaccggctac aagaccctgg acgagggcca
      961 gcacgtcgag ttcgacgtcg cccccggccg caagggcgaa gaggcccaga acgtccgcgt
     1021 ggtctgatag ccaacgcgac cgctcgcagg gccgccggcc tcttggccgg cggtcctttg
     1081 cgttctggcc gggaatcggg cgccagatgg ggcacaatgg cgccatgagc aatccggccc
     1141 tgaacccgaa gatcttccag aaggagacgg cggcgaacca gcagccgagc gccttcgagc
     1201 ctggctgggg ctccccggcc gacgagctgc cgccgaacgt gttcgacgcc caggccggcg
     1261 tggccggcgc cgccccgacc cgcccgccgg tctccgccgg caccggcgac acgatgcgga
     1321 tcagcggcac catgaccgcg accgccatct tgctgctgct gctcatggtg gcgggctact
     1381 tcggctggca ggcgctcgac ctccaccaga ccggggtgac gccggccggc gagaaggtgt
     1441 acgccgtcaa tcccccggcc tggatcttcg ccgtctggat cggcacgttc atcctggcga
     1501 tggtcacgat cttcaagccc cggctggcac gggtcacggc gcccatctac gccatcggcg
     1561 agggcctcgt cctcggatcg gtctccgcca tcttcgaagc gtcctacccc ggcatcgtgg
     1621 tgcaggccgt gctgctcacc ttcggcgtct tcatcacgat gatggtcctc ttcgccaccg
     1681 gcacgatccg ggtcaccgag aagctccgca tgtgcatctt cgcctccatg ggggcgatcc
     1741 tcctcgtgta cctggccgac atcatcgcca gcctcttcgg gagctcgctg ccgatcgtca
     1801 actcgtcgtc gccctggggc atcggcttca gcgtcatcgt ggtcatcgtc gcctcgctga
     1861 acctgctgtt gaacttcgac ttcatcaagc aggccgtcga ccaccaggcg ccgcggtaca
     1921 tggagtggtt cgccgccttc ggcctcatgc tcgccctcgt gtggctctac ctggagatcc
     1981 tgcgcctgct ggccaagctc cggaactgag ccgggcggtt cccgcaccgg ccagccgacc
     2041 tcgtcgtcct cgggcagaat ggcccgacca gcgtcgagga aggctgaccg agtgagcgac
     2101 acgattgcat gttccagctg cggcaccgag gtgccggtgg cggcctcgtt ctgcacgggc
     2161 tgcggccagc cgatggcctc cgccgccggg ccgccacccc caccgccccg cgcgctcgag
     2221 ccggtaccga ccgacgcgcc cgacgtcacc caggtggtga cccccggcct ccacgacgcc
     2281 acccaggtca ccccaccgcc gccgagcacg ccggcgccgc cgccctacgc cccacccgct
     2341 cccccgccgc ccaccgcgcc ggcctcgggc ccgtggggcc cgagctcggc ggagccgccc
     2401 accgccgcgc cctggacgcc gcccaccgcc gccgccccgg gagcaccgac ggcacccccg
     2461 ccgcccacct ggcagcagcc caccccgccg cccagcccgg gcacctaccc gccggtgggc
     2521 ggtgtcgccg ccggcagctg gcagaacccg gccgcgcccg gtgctgctcc ctggggcgct
     2581 gctcctgagg cggcaccagc cgcggccggc ggctcggtgc tcggcggcgt ggtcgggctc
     2641 atcggcgccg tcctggccct cgtcgggatc ttctcgggct gggtgaagct ggggaacacc
     2701 gggacggtca ccgcgtggtc gctcaccgaa ggcgagggac tgctcaagac ccagacgccc
     2761 tacgtgatcc tcgccttcgc ggcggcggcc gtcatcgtct cgctcgtgct cttcacgggc
     2821 atcgcccgtc cggtggctcg gatcctggcc gtgctcctcg gcctcgccat catcggcgtc
     2881 accgccgcga actggaacga gatcgccagc ttcgtgaccg acaacttccc atcgagcttc
     2941 aaggcctcca ccgcggtcgg cttctacctc acgatcgccg gtggcgtgct catcatcgtc
     3001 tcggcgctgc tgccggcgaa gaagaagcca tgagcgagac gcacgccctg gcgggcacgg
     3061 gcgcgctcgt caccgggggc ggcagcggca tcggcctggc catcgccacc cggctcgcct
     3121 ccgacggggc caccgtcacc atctgcgggc gctcggcgga gcggctcgat gccgccgtcg
     3181 ccgccaacgg tctcgcggat ggtccgggct cgctgcacgc cgtggtggcc gacgtcaccg
     3241 acgaggacgc cgtcgccacc gccgtg
//
LOCUS       DATMFS010000338         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_198018, whole genome shotgun sequence.
ACCESSION   DATMFS010000338 DATMFS010000000
VERSION     DATMFS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198018"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VNQ33_06135"
     CDS             <1..788
                     /locus_tag="VNQ33_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97720.1"
                     /translation="LAGEAVSCLYQGTDGSNPAGFTTTLAPGVAVTQTIVADDESTDS
                     PADANDTAEVSQAVIESIDAGLKAVSAVTEAGFSAPFTLIVKMADVLFGLSTNCNNQP
                     NVFQLGAATADGQGTNNTSWAVFDGCSGTCGGLANVYSSPWESSAPDSDAQMSTEAVQ
                     LAPSTVMYDGQPLWLDQQPVGTGCGVGNGSDSNTSGCTSENMISLRWLTSPPCPWTNG
                     LEAGDGTGVQGNVSWCYQDPPASPSIPSCGTDNASCLAYEPDS"
     gene            785..1558
                     /locus_tag="VNQ33_06140"
     CDS             785..1558
                     /locus_tag="VNQ33_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWJ97721.1"
                     /translation="MTIRAAAGALAALAVTSTIATGCSDAGPPVVTVAIIDTGIARDA
                     PLGDMVDVDASSSVVPGGVDDEDGHGTEMAAVVHAAAPDARLVAVKAVGDDGTTDQRL
                     ATAIGRARSAGADVILLSMAGAEPLPRTRAAIERAGRGDVLVVAAAGNDGLDLDDHPA
                     YPAAYDEPNLIAVAAIDRDGQLLGTSNRGPTVTARGQGSGVSTCTLDGRPATTGATSA
                     AAALVAGTAARRLRTDGPLEDIRLQLESKPAFATAEPCS"
     gene            1629..>2149
                     /locus_tag="VNQ33_06145"
     CDS             1629..>2149
                     /locus_tag="VNQ33_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97722.1"
                     /translation="MIHVPKPQIEALLFDLGGVVMGIDFDRAVSSWEDAAGLPPGTLV
                     GRFTMDDPYERHERGHLDGVDYLAHLRTTLAVDLTDEQLSAGWNDIYLGPIDGVGEIL
                     SRAADALPTYALTNTNALHQAVWEPRFATEMAPFRTIFSSHLIGLRKPEEAAFRHVAA
                     AIGVPLERILFFD"
BASE COUNT          342 a          846 c          679 g          282 t
ORIGIN      
        1 ccctcgccgg cgaagccgtg agctgcctgt accagggcac cgacggctcg aaccccgccg
       61 gcttcaccac gaccctcgcc cccggtgtcg cggtgaccca gacgatcgtc gccgacgacg
      121 agagcaccga ctcgcctgcc gatgccaacg acaccgccga ggtctcccag gcagtcatcg
      181 agtccatcga cgccggcctc aaggcggtct cggccgtcac cgaagcaggg ttcagcgcac
      241 cgttcaccct catcgtcaag atggccgacg tcttgttcgg cctcagcacg aactgcaaca
      301 accagcccaa cgtgttccag ctgggagcgg cgaccgccga cggccagggc acgaacaaca
      361 cgtcgtgggc cgtcttcgac gggtgcagcg gcacctgcgg cgggttggcc aacgtctaca
      421 gctcgccgtg ggagagctcg gcacccgact cggacgccca gatgtccacg gaggcggtgc
      481 agctcgcccc cagcaccgtg atgtacgacg gccagccgct gtggctcgac cagcagcccg
      541 tcgggacagg gtgcggcgtg ggcaacggca gcgactcgaa caccagcggg tgcacgagcg
      601 agaacatgat ctcgctgcgg tggctcacgt ctcccccgtg cccgtggacg aacggcctcg
      661 aagccggcga cggcactggc gtccagggga acgtctcgtg gtgctaccaa gacccgcccg
      721 cctcgccgtc gatcccctca tgcggcaccg acaacgcctc gtgcctggcc tacgaaccgg
      781 attcgtgacc atccgggcgg ccgccggagc gctggccgcc ctggcggtca cctccacgat
      841 cgccaccggt tgcagcgacg ccggcccccc ggtcgtcacc gtcgccatca tcgacacggg
      901 catcgctcga gacgctcccc tcggggacat ggtcgacgtc gacgcgagca gcagcgtcgt
      961 acccggtggg gtcgacgacg aagacggcca cggcacggag atggccgccg tcgtccacgc
     1021 cgccgcgcca gatgcccgcc tcgtggcggt gaaggccgtc ggcgacgacg gcaccaccga
     1081 tcagcgcctg gccaccgcca tcggccgtgc ccgttcggca ggcgccgacg tgatcctgct
     1141 ctcgatggcc ggcgccgagc cgctcccccg cacccgggcc gccatcgagc gggccggccg
     1201 aggcgacgtg ctggtggtcg ccgccgccgg caacgacggc ctcgacctcg acgaccaccc
     1261 cgcctacccc gctgcgtacg acgagcccaa cctgatcgcc gtcgccgcca tcgaccgaga
     1321 cggccagctg ctgggcacct cgaaccgagg cccgaccgtc acggcacgag gccagggatc
     1381 cggcgtctcg acctgcaccc tcgatggtcg acccgccacg accggcgcca cctccgccgc
     1441 cgccgccctc gtcgccggca ccgccgcccg ccgcctccgc accgacgggc ccctcgaaga
     1501 catccgcctc cagctcgagt ccaaaccggc gttcgccacc gctgaaccct gcagctgatc
     1561 catccgacga gaccgctgcc gcgcgggtag aggagtccgg gggccagcag tcgtgcgaag
     1621 ttggggccat gatccacgtt ccgaaaccgc agatcgaggc actgctcttc gacctcggcg
     1681 gcgtggtgat ggggatcgac ttcgaccggg ccgtgtcgtc ctgggaggac gccgccgggc
     1741 tgccgccggg caccctcgtc gggcgcttca ccatggacga tccctatgag cgccacgagc
     1801 gcggccacct cgatggcgtc gactacctcg cccacctgcg gaccacgctg gccgtcgacc
     1861 tcacggacga gcagctgtcc gccgggtgga acgacatcta cctcggcccc atcgacggag
     1921 tcggcgagat cctgtcccga gccgccgatg cgctgcccac ctacgcgctc accaacacca
     1981 atgccctgca ccaggcggtg tgggaacccc gcttcgcgac ggagatggca ccgttccgca
     2041 ccatcttcag ctcgcacctg atcgggctcc gcaaacccga ggaggccgcc tttcgccacg
     2101 tcgccgccgc gatcggcgta ccgctcgagc gcatcttgtt cttcgacga
//
LOCUS       DATMFS010000339         8670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_220957, whole genome shotgun sequence.
ACCESSION   DATMFS010000339 DATMFS010000000
VERSION     DATMFS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220957"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..870
                     /locus_tag="VNQ33_06150"
     CDS             319..870
                     /locus_tag="VNQ33_06150"
                     /inference="COORDINATES: protein motif:HMM:NF045184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97723.1"
                     /translation="MSDTDLQPPTGARLDLLKRLGFGELEHDSHVPFLSHLIGTRRTL
                     VEWGERPAVCDAGLFHSAYGTEYFPVDEPVDRTEVQAVIGAEAEEIAHAWCTIRRDTI
                     ELGEAGGPATVVDRTTDERIEISDQLLADIAVLWAADTVEQIARMTPDERAFATTLDR
                     VLHLASPAAREAVATTIAGLEAS"
     gene            1003..1500
                     /locus_tag="VNQ33_06155"
     CDS             1003..1500
                     /locus_tag="VNQ33_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWJ97724.1"
                     /translation="MHTLLLNASYEPLTVISVHRAVVLVLADKADLVAAGEGHFRSPS
                     TSVPTPSVIRLRGFVRVPYRSTLPLNRRNLIARDDGRCAYCTGRGDTVDHVVPRSRGG
                     QHVWTNVVAACQPCNGHKGDRLLQELGWQLRTTPAAPRGWSWMLVGVGTTDPAWSQWL
                     DPVGA"
     gene            complement(1699..3333)
                     /locus_tag="VNQ33_06160"
     CDS             complement(1699..3333)
                     /locus_tag="VNQ33_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWJ97725.1"
                     /translation="MATVHARGVSLSFGAGPVLAGVSFTVAPGQRIGVVGPNGTGKST
                     LLKVLAGQLAPEAGSVTAAPPNASVGLLPQEPDRDDGESVAGFIGRRTGVAAATREMD
                     AATAALSTGEPGADDRYSDALERWLALGGPDLDARIGQTLADLGLDGAIVDQPTATLS
                     GGQAARVSLAATLLSRFDVFLLDEPTNDLDFAGLAILEEFLVGLQAAAVVVSHDRAFL
                     ERVVTHVLDLDEHTHEATLYAGGWSAYLADKATARQHAEEGFATYQAKKAELSARAKT
                     QREWSVQGVKKAKTSGETDKFIKHWNTSSSEKVAHKAKATEKAMERLDVVDKPWEGWE
                     LRFEIGQAARSGDVVATLAGAVVERGSFRLGPVDLEIGWADRVAILGANGAGKTTLLD
                     ALLGRTELTSGEAYLGPGVVIGELSQRRDRFSSEASLFAVMSDATGQQANEVRSLLAK
                     FGLTAEHVNKPSRLLSPGERTRANLALFMAEGVNCLVLDEPTNHLDLVAIEQLEQALG
                     RFVGTVLLVSHDRAFLDRVETTRRITVADGQVTETS"
     gene            complement(3523..4527)
                     /gene="rlmB"
                     /locus_tag="VNQ33_06165"
     CDS             complement(3523..4527)
                     /gene="rlmB"
                     /locus_tag="VNQ33_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWJ97726.1"
                     /translation="MTPPRGGRPPAKGGKGGGAKGGKGRGAPPQGRGSSSRSGGSTEA
                     DRARKPGAGTGGGRRGGGGGGRNPLAQMGPQGKAPRASARTGLGGEQVEGRQAVRELL
                     LANRRDTREIWMAQDLDPAPVLEDIRELAAEARVVVKEVGRGKLDAAARTDAPQGIIA
                     FAKPLPEADLDALIARRPGRPAPFLLCSDGITDPGNLGALLRSAECAGVTGVVLPQHR
                     ALHVTPTVAKAAAGAVEHLPMTTVGGMPTAIKTMKDAGIWVVGLDERADKTLWDLTMD
                     DAPVALVLGAEGPGLSRLVRERCDELVSIPMRGRVASLNVGTAGTLACFEIARARRQR
                     "
     gene            complement(4524..5000)
                     /gene="ispF"
                     /locus_tag="VNQ33_06170"
     CDS             complement(4524..5000)
                     /gene="ispF"
                     /locus_tag="VNQ33_06170"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWJ97727.1"
                     /translation="MQVRVGQGFDVHPFVDDADRPLVLGGVTFPSERALKGHSDADAI
                     AHACTDALLGAAGLGDIGQHFPDTDPAWEGADSIELLAEATRRVRAAGWEPGNVDASV
                     VTDHPKLAPAREQMQQNLSDAVGAPVTVKGRRPEGLGALGRGEGVACWAVAVVVKP"
     gene            complement(5000..5650)
                     /locus_tag="VNQ33_06175"
     CDS             complement(5000..5650)
                     /locus_tag="VNQ33_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWJ97728.1"
                     /translation="MTTARESSSPPVWVIVVAAGSGSRFGGPKQHAPLAGRRVVDWSI
                     DAARSVADGVVLVVAAADEGADEPAADAVVVGGATRSESVRAGLAAVPADAAVVLVHD
                     GARPLASHALFRSVADAVLAGADAVVPAVAVVDTVTDLHGVPVDRDALRTVQTPQGFD
                     ARALRTVHASRPEATDDASLVVAGGGRLRPVQGERWNLKITEPDDLVVAEAILEQR"
     gene            5713..6372
                     /locus_tag="VNQ33_06180"
     CDS             5713..6372
                     /locus_tag="VNQ33_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ97729.1"
                     /translation="MVDIGLLQDVQLLDELGDDEMARVAASAETHRYLRGDVVFAEGD
                     EPDFLCVVASGRIAIAKRSIDGRESMVALMERGDVFGEFGLFDQLGRSAEARALEPSE
                     LIVLPYAPLKAVYESRPALLWGVVRLLAARLRNTDAALADSVFLDVTGRTAKRLLELA
                     GEHDEFALPITQEELAGMVGASRERVNKAIASFVRLRWIEQQDRRYRITNREELERRA
                     R"
     gene            complement(6975..8030)
                     /gene="disA"
                     /locus_tag="VNQ33_06185"
     CDS             complement(6975..8030)
                     /gene="disA"
                     /locus_tag="VNQ33_06185"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HWJ97730.1"
                     /translation="MPASDLRATLSSVAPGTALREGLERIMRGRTGALIVIGQDRVVD
                     SISTGGFALDVPFTATGVRELAKMDGAIILDSSATRIMRAAVHLMPDPSIPTTESGTR
                     HRTAERVARSLNVPVVAVSHRMNTITVYVGDHKHEIEPVPRLVNRANQAISTLERYRT
                     RLDTDAAALSTLEIEDLVTIRDVINVVQVLEGVERIAEELERYIVELGDEGRLVRLQL
                     VELVGGVQHDRRHIVRDYRGDDKTVPLDQVLDDLSSLTTEDLIDPSEVAHAMRLPTTA
                     VDASIAPRGYRMLAKIPRLPDQVIQNIITRFGNLQKIMRATIDDLDDVEGVGAMRAKS
                     IKEGLSRLAESAILDRY"
     gene            complement(8071..>8670)
                     /locus_tag="VNQ33_06190"
     CDS             complement(8071..>8670)
                     /locus_tag="VNQ33_06190"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HWJ97731.1"
                     /translation="KNRFGPVDEIGCFDLVDAGIVEVPDPTGLFVSRHESPVAGTCVT
                     VTMEGRRPLLAEVQALTVAATTPSPRRSSSGLDSSRVSMLLAVLSKTGVSLGNQDVYT
                     ASVGGARLHEPAVDLAVALAVISAAGGSPLPGGLVAIGEVGLAGEVRPVRGLEARLRE
                     AARIGFTHAIVPTGTESLDRVGLKVLRTGDLAAALRALP"
BASE COUNT         1173 a         3176 c         3118 g         1203 t
ORIGIN      
        1 ccgcttgccg tcgagccgga cctcgagggg ctcctcgacg atgacctggt cgggagatcg
       61 gcccgactcg ccgtcggcca ccttccgcac gatctgcgac accgtgcgcc cacgacccat
      121 gcccgccaac ctaccgacca cccccgattt cgggtcacct gagcaccccc acggtgcaca
      181 ggtgacccga aatcagccgc gcccgcggcc tggtcgacgg caccgacgtc ctcctcgccg
      241 ccgactgggc caaggctgag ctcgacgcct ggtgatcgac ggtgacaggc ggacgaccgc
      301 gcgcgaggct cgatggcgat gagcgacacc gacctgcagc cgcccaccgg agcccgcctc
      361 gacctgctga agcgcctcgg cttcggcgag ctggagcacg actcgcacgt gcccttcctc
      421 tcgcacctca tcggcacccg ccgcacgctg gtcgagtggg gtgagcggcc ggcggtgtgc
      481 gacgccgggc tgttccactc ggcgtacggg accgagtact tcccggtgga cgagccggtg
      541 gaccggaccg aggtgcaggc ggtgatcggc gccgaggccg aggagatcgc ccacgcctgg
      601 tgcaccatcc ggcgcgacac catcgagctg ggcgaagccg gggggccggc cacggtggtc
      661 gaccgcacca ccgacgagcg catcgagatc agcgaccagc tgctggccga catcgccgtc
      721 ttgtgggccg ccgacacggt cgagcagatc gcccggatga cccccgatga gcgtgcgttc
      781 gccaccacgc tcgaccgggt gctgcacctc gcctcacccg cggcgcgcga ggcggtggcg
      841 acgacgatcg cggggctcga ggcgagctga cctcgggcgc ccagcacggc gccaccggca
      901 aaatcccttg gtccccaccg gcccggcgca gcatcgtgcc cccggcggga tgccacctct
      961 ggcgggccgc cacccggcac actgaggaag ggagacgaac cgatgcacac gctgctgctg
     1021 aacgccagct acgagccgct cacggtcatc tccgtccatc gcgccgtggt gctggtgctg
     1081 gccgacaagg cggacctcgt cgccgccggc gagggccact tccggtctcc ctccacgtcg
     1141 gtgcccacgc cgtcggtcat ccgcctgcgc gggttcgtgc gggtgccgta ccggtccacg
     1201 ctgccgctga accggcgcaa cctcatcgca cgcgacgacg ggcggtgcgc gtactgcacc
     1261 ggacgcggcg acacggtcga ccacgtcgtg ccccgctccc gcggcggcca gcacgtgtgg
     1321 accaacgtgg tcgccgcctg ccagccgtgc aacggccaca agggcgaccg gctcctccag
     1381 gagctgggct ggcagctgcg gaccacgccc gcggcgcctc gcggctggtc ctggatgctg
     1441 gtcggcgtcg ggaccaccga tcccgcctgg tcccagtggc tcgaccccgt cggcgcctga
     1501 tccccgatca ggggtccgac cagcccgccc gtgcgtccgc ccggctggcc gcacggccca
     1561 cccgcccggc cggacgcacg aaccccagcg aactgcgcag gcgagacgca cccatcggcg
     1621 ctgcggtccc gcatcggcgt cttcgcctgc gcagttcggc cgaagttcag cggggctggc
     1681 ggtaagggag ctcgaccgtc acgacgtctc cgtgacctgg ccgtcggcga cggtgatgcg
     1741 gcgggtggtc tcgacccggt cgaggaaggc ccggtcgtgg ctgaccagga gcacggttcc
     1801 gacgaagcgg cccagcgcct gctcgagctg ctcgatggcg acgaggtcga ggtggttggt
     1861 gggctcgtcg agcaccaggc agttcacgcc ctcggccatg aacaaggcga ggttggcccg
     1921 ggtgcgctcg ccgggcgaca ggaggcggga cggcttgttc acgtgctcgg cggtgaggcc
     1981 gaacttggcg agcagcgagc ggacctcgtt ggcctgctgg ccggtggcat cgctcatcac
     2041 cgcgaacagg gatgcctcgc tggagaaccg gtcccggcgc tgcgacagct cgccgatcac
     2101 gacacctggg ccgaggtagg cctcgccgct cgtcagctcc gtgcgaccga ggagggcatc
     2161 gagcagcgtg gtcttgccgg cgccgttcgc cccgaggatg gcgacgcggt cggcccagcc
     2221 gatctccagg tccaccggcc cgaggcggaa ggacccccgc tccaccaccg caccggccag
     2281 cgtcgccacc acgtcgccgg agcgggcggc ctggccgatc tcgaaccgca gctcccagcc
     2341 ctcccacggc ttgtcgacga cgtcgagccg ctccatggcc ttctccgtgg ccttggcctt
     2401 gtgggcgacc ttctcggatg acgacgtgtt ccagtgcttg atgaacttgt cggtctcgcc
     2461 ggacgtcttc gccttcttca cgccctgcac cgaccactcc cgctgggtct tggcccgggc
     2521 gctgagctcg gccttcttgg cctggtaggt ggcgaagccc tcctcggcgt gctggcgggc
     2581 cgtggccttg tcggccaggt aggcagacca gccaccggcg tagagcgtcg cctcgtgggt
     2641 gtgctcgtcg aggtccagca cgtgggtgac gacccgctcg aggaacgccc ggtcgtggct
     2701 caccaccacg gcggccgcct gcagcccgac gaggaactcc tcgaggatgg cgaggccggc
     2761 gaagtccaag tcgttggtgg gctcgtcgag caggaacacg tcgaagcggc tgagcagcgt
     2821 ggcggcgagc gacacccgag ccgcctggcc gcccgagagc gtggcggtcg gctggtcgac
     2881 gatggcgccg tcgaggccga ggtcggccag cgtctggccg atgcgggcat cgaggtccgg
     2941 gccgccgagc gccagccacc gctcgagcgc gtcgctgtag cggtcgtccg cgccgggctc
     3001 gcccgtggac aacgccgcgg tcgccgcatc catctcccgc gtcgcggcgg cgacgccggt
     3061 gcggcgcccg atgaacccgg cgacggactc gccgtcgtcg cggtcgggct cctgcggcag
     3121 caggccgacg gacgcgttcg gcggcgccgc ggtgaccgac ccggcctcgg gcgccagctg
     3181 cccggccagc accttgagca gggtggactt gccggtaccg ttcgggccga cgacgccgat
     3241 ccgctgcccg ggtgcgacgg tgaacgacac gccggcgagc accggccccg cgccgaacga
     3301 gagggacacc ccacgggcgt gcacggttgc catgacccgc ccagtctccc cgaccacctg
     3361 accgcccacc ccatcagatt tcgggtcgtt cacgccccac cacggtgcac aggcgacccg
     3421 aaacggccag aggtccccgc cgtttcacat ttcgggtcgc tcgatcaccg tggaggtgca
     3481 caggcgaccc gaaatcgggg cggggccggc tcggactcgg ggtcagcgct ggcggcgggc
     3541 acgggcgatc tcgaagcagg cgagggtgcc ggcggtaccg acgttcaagc tggcgacccg
     3601 gccccgcatg gggatcgaca ccagctcgtc gcagcgctcg cggaccaggc gggacaggcc
     3661 ggggccctcg gcaccgagga cgagcgccac gggcgcgtcg tccatcgtga ggtcccacag
     3721 cgtcttgtcg gcccgctcgt cgagcccgac gacccagatg ccggcgtctt tcatcgtctt
     3781 gatcgccgtc ggcatgccgc cgaccgtcgt catcggcagg tgctcgaccg caccggccgc
     3841 cgccttggcc accgtcggcg tcacgtgcag agcgcggtgc tggggcaaca cgacaccggt
     3901 cacaccggcg cactcggctg agcgcagcag ggcgccgagg ttgccggggt cggtgatgcc
     3961 gtcgctgcag agcaggaagg gcgccgggcg accgggccgc cgggcgatga gcgcatcgag
     4021 gtcggcctcg ggcagcggtt tggcgaaggc gatgatcccc tggggcgcgt cggtgcgggc
     4081 ggcggcatcg agcttgcccc ggcccacctc cttgacgacg acccgtgctt ccgcggccag
     4141 ctcgcggatg tcctcgagca ccggtgccgg gtccaggtcc tgggccatcc agatctcacg
     4201 ggtgtcgcgg cggttggcga gcagcagctc gcgcacggcc tggcggccct cgacctgctc
     4261 gccgcccagc ccggtgcggg ccgacgcccg cggcgccttg ccctggggtc ccatctgcgc
     4321 cagcgggttg cgtccgcccc cacccccacc ccggcggcca ccgccggtgc cggcgcccgg
     4381 cttgcgggcg cgatcggcct cggtgctgcc gcccgagcgt gacgagctgc cccggccttg
     4441 cggcggagcg ccccggccct tgccgccctt cgcccctccg cccttgccgc ccttggccgg
     4501 tggccggcca ccacgcggcg gggtcacggc ttcaccacca cggcgacggc ccagcaggcc
     4561 accccctcgc cgcggccgag ggcaccgagt ccctcgggtc gacggccctt cacggtgacg
     4621 ggagcaccga cggcatcgga caggttctgc tgcatctgct cccgggcggg cgccagcttc
     4681 gggtggtccg tgaccaccga ggcatcgacg ttgccgggct cccatcccgc cgctcgcacc
     4741 cggcgggtgg cttcggccag cagctcgatg ctatcggccc cctcccacgc cgggtcggtg
     4801 tcggggaagt gctggccgat gtcgcccagg cccgcggcgc cgagcagggc gtcggtgcag
     4861 gcgtgggcga tggcgtcggc gtcgctgtgg cccttcagcg cccgctcgct ggggaacgtg
     4921 acaccgccga gcacgagcgg tcgatcggcg tcgtcgacga acgggtggac gtcgaagccc
     4981 tgaccgacgc ggacctgcat caccgctgct ccaggatcgc ctcggccacc acgaggtcgt
     5041 cgggctcggt gatcttgagg ttccagcgct cgccctgcac ggggcgcagc ctgccacctc
     5101 cggccaccac cagcgaggcg tcgtcggtcg cctcggggcg ggaggcgtgg acggttcgca
     5161 gcgcgcgggc atcgaagccc tgcggtgtct ggacggtgcg gagcgcgtcg cggtcgaccg
     5221 gcacgccgtg gaggtcggtc accgtgtcca ccaccgcgac cgctggcacc accgcatcgg
     5281 ccccggccag cacggcgtcg gccaccgagc ggaacagcgc gtgcgaggcc aacggacgag
     5341 cgccgtcgtg caccagcacc acggcggcat cggcaggcac ggccgccagc ccggcgcgca
     5401 ccgactcgga gcgggtcgca ccgcccacga cgacggcatc ggccgcgggt tcgtcggcgc
     5461 cctcatcggc agcggcgacc acgagcacca cgccgtcggc caccgagcgg gctgcgtcga
     5521 tggaccagtc gacgacgcgc cggccggcca gcggcgcgtg ctgcttgggt ccgccgaagc
     5581 gggaccccga ccccgcggcc accacgatca cccagacggg cggggacgag gattcgcggg
     5641 cagtggtcat cgctcaacgg taggacacga ggatgtccca gccatcggtc ccgaaatcgg
     5701 tacgatgccc gcgtggtcga catcggtctg ctccaagacg tgcaactcct cgatgagctc
     5761 ggcgacgacg agatggcgcg ggtggcggct tcggcggaga cccaccgcta cctgcggggc
     5821 gacgtcgtgt tcgccgaggg tgacgagccc gacttcttgt gcgtcgtcgc ctccggccgc
     5881 atcgccatcg ccaagcggtc gatcgacggc cgggagtcga tggtcgcgct gatggagcgc
     5941 ggcgacgtgt tcggcgagtt cggcctgttc gaccagctgg gccgctccgc cgaggcccgg
     6001 gccctggagc cgtccgagct gatcgtgctg ccctacgcgc cgctgaaggc ggtgtacgag
     6061 tcccgaccgg cgctgctctg gggcgtggtg cggctgctcg ccgcccgcct ccgcaacacc
     6121 gatgccgccc tagccgactc cgtgttcctc gacgtgaccg gacgcacggc gaagcgtctg
     6181 ctcgagctgg ccggcgaaca cgacgagttc gccctgccca tcacccagga ggagctcgcc
     6241 ggcatggtcg gcgcctcccg tgagcgcgtc aacaaggcca tcgccagctt cgtccggctc
     6301 cgctggatcg agcagcagga ccgccgctac cgcatcacca accgcgaaga gctcgagcgc
     6361 cgcgcccgct gaggcgacac gtcggcggac gctcgaacga cacgcggaag gacccgatga
     6421 ccacgaagcc cccgggtgat cccggctggg gccgcccgca ggacgtcctc ctcggggcca
     6481 tcgtccccca gctcgccatc cgacgcaccc ggggtgccgc ctcgcccttg gctgcgaccc
     6541 ggaccgtgtt cgtctcgttc gtcacctcgc tcctcctcat cggcgtcgtg gtcctgatcc
     6601 tgtccggggg ctggtcgacg gacggcggct cctcgcccgt cgcctggtgc gcggcatcgg
     6661 ccgtcatcgg agtggtgagc gttgccgtcg gtcatgcgtt ccgacggacg ctgccgtgcg
     6721 gctcgcccga gacgctgctc ggtgcctacc ggacccgctt cttcctccgc atcgcctgga
     6781 acaacgccgc ggcgctgagc gggttcgtga tggccttcct ggccgagagc ccctggccgt
     6841 acttcaccgc cctccccatc gccctcgtcg ggcacgggct caccgcgccc acgtggcagc
     6901 acatctcaag gccagagcgt gaggggcccg tgggccgcct ggaccacggt gaagcggcgg
     6961 tgatcgagca gcggtcagta gcggtcgagg atggcggact cggcgaggcg gctgaggcct
     7021 tctttgatcg acttggctcg catggcgccc acaccctcga catcgtcgag gtcgtcgatg
     7081 gtggcccgca tgatcttctg gaggttgccg aaccgggtga tgatgttctg gatcacctgg
     7141 tccggcaggc gggggatctt ggccagcatc cggtagcccc ggggggcgat gctggcgtcg
     7201 accgccgtgg tgggcaggcg catggcgtgg gccacctcgg acgggtcgat gaggtcctcg
     7261 gtggtgagcg acgacaggtc gtcgagcacc tggtcgagcg gcaccgtctt gtcgtcgccc
     7321 cggtagtcgc ggacgatgtg gcggcggtcg tgctggacgc cgcccaccag ttccacgagc
     7381 tggaggcgga cgagccggcc ctcgtcgccg agctccacga tgtagcgctc gagctcctcg
     7441 gcgatgcgct ccacgccctc gagcacctgc acgacgttga tgacgtcacg gatggtgacc
     7501 aggtcctcga tctccagggt ggagagggcg gcggcgtcgg tgtcgaggcg ggtgcggtag
     7561 cgctccaggg tggagatggc ctggttggcc cggttgacca ggcggggcac gggctcgatc
     7621 tcgtgcttgt gatccccgac gtacaccgtg atggtgttca tgcggtgcga caccgccacc
     7681 accgggacgt tgagcgaacg ggcgacgcgc tcggcggtgc ggtggcgggt gccggactcg
     7741 gtggtgggga tcgacgggtc gggcatgagg tgcacggcgg cgcgcatgat ccgggtggcc
     7801 gaggagtcga ggatgatcgc gccgtccatc ttcgccagct cgcggacgcc ggtggcggtg
     7861 aaggggacgt cgagggcgaa gccgcccgtg gagatcgagt cgacgacgcg gtcctggccg
     7921 atgacgatca gcgcgccggt gcggccccgc atgatgcgtt cgagaccctc acgcagcgcc
     7981 gttccgggcg cgacggagct cagggtcgcc cgcaggtctg aagccggcac gacgtctctc
     8041 gctctcgctc acgtagtggt cgggctcatc ctagggcagc gcccggagcg cagcggcgag
     8101 atctccggtt cgcagcacct tcaggccgac ccgatcgagg ctctcggttc cggtggggac
     8161 gatcgcgtgg gtgaagccga tgcgcgccgc ctcgcgcagc cgggcctcga ggccgcgtac
     8221 cgggcggacc tcgccggcca ggccgacctc accgatcgcg acgagtccac ccggcagcgg
     8281 cgatccgccc gcggcggaga tcacggccag cgcgacggcg aggtcgacgg ccggctcgtg
     8341 cagccgcgcc ccgccgaccg aggccgtgta gacgtcctgg ttgcccagcg agacccccgt
     8401 cttgctcagc accgcgagca gcatcgagac gcgcgaggag tccagaccgc tggaggagcg
     8461 tcgcggcgaa ggcgtggtgg ccgcgaccgt gagcgcctgc acctcggcca gcagcggccg
     8521 acgaccctcc atcgtgaccg tgacgcacgt gcccgcgacc ggcgactcgt ggcgcgacac
     8581 gaacaggccc gtgggatcgg gcacctcgac gatgccggcg tcgacgaggt cgaagcagcc
     8641 gatctcgtcg accgggccga agcggttctt
//
LOCUS       DATMFS010000340         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_57750, whole genome shotgun sequence.
ACCESSION   DATMFS010000340 DATMFS010000000
VERSION     DATMFS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57750"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1204)
                     /locus_tag="VNQ33_06195"
     CDS             complement(119..1204)
                     /locus_tag="VNQ33_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97732.1"
                     /translation="MTAAAASVPVLPVADLVRAVAWYERLGFRVRAFFDGYAILGLDG
                     AELHLNELPGLPPATESVSGAYLRVADADAVFAVWTAMGAPTVAPPEDQPYGIREFAT
                     EDLDGNLWRIGAVIPGGPADGYLPPDAPAVEAAPAPAVPADALDSAAGAPATPAPPEQ
                     EVPSGPAPDAAGSRTDGGAGTGQESWPGIVADGRRCAGCGLVNGELAARAIGAQVRDE
                     VHALGELLAAAGDDAVRQRPAPVTWSALEYGVHVRDLLSVFAERIIRTLAEHDPQLGW
                     WDHEAAIEDGFANESDVSAVVDDLGRNAAKLSEALRLVSDDDWDRPATRRAGERMTIE
                     VQARFALHEVVHHRADAVASLAAAGAS"
     gene            complement(1201..2304)
                     /locus_tag="VNQ33_06200"
     CDS             complement(1201..2304)
                     /locus_tag="VNQ33_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWJ97733.1"
                     /translation="MTDAATGAAGLTDLVTTSRIIICSGSGGVGKTTTAAVVAMEAAN
                     VGRRAVVVTIDPAKRLADALGLDGIGNTPKRIDGPWPGELWAVMLDTKSTFDDLVTTY
                     SAEPEQAERILANRFYKNISGALSGTQEYMAMEKLYELHNSTDFDLVVVDTPPSRNAL
                     DFLDAPKRLTRFLDHRLYRVIMAPTRGVMKAVNVAAQAFVRQVSKVVGASVFEDAVTF
                     FQAFDGMEAGFKDRAEHVLQLLTSPATSYVLVASPKRDTVAEARFFAEKLGEADIPIA
                     ALVVNRMHPHFTDELPEALRERSETFAGSDLGGLYRNLADFSLVAHREEEHLAGLAQM
                     VEPAPVIRVPFLRTDVHDLTGLALVGDHLFGRS"
     gene            complement(2301..>3014)
                     /locus_tag="VNQ33_06205"
     CDS             complement(2301..>3014)
                     /locus_tag="VNQ33_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HWJ97734.1"
                     /translation="RTLTPDEALVEYLEDSDMSRISKRLMTSGALDMVSTAIPGIRDI
                     LVLGKIKQMERAGLADLLILDAPAAGHAISFLQSASGLADAVPIGPINNQAKDVLALL
                     GDPERCQVMLVTLPEETPVNELVETAYQLEDRVGISLGPVVVNGLYPPLTGLDADPSA
                     AAEAAGASLRPGEADALDAAATFRRERMELQAEQVGRLAEALPLPQLSLPYLFDAELG
                     PAQLEQLAASMLDGIGGLS"
BASE COUNT          404 a         1009 c         1181 g          420 t
ORIGIN      
        1 gtggcggtgg cctgcagccg ccccaccctg acccgcctgc tgcacaccac cggcttcgac
       61 cggatcgtgc cggtcaccga gacgctcgac gcggctgtgg ccgccgtcgc atcgccggtc
      121 agctagctcc ggcggcggcc agggaggcca cggcgtccgc ccggtggtgg acgacctcgt
      181 ggagggcgaa gcgcgcctgg acctcgatgg tcatgcgctc gccggcccgg cgggtggcag
      241 ggcggtccca gtcgtcgtcg ctcaccaggc gcaacgcctc ggagagcttg gcggcgttgc
      301 ggcccaggtc gtcgaccacg gcgctcacgt cggactcgtt ggcgaagccg tcttcgatgg
      361 cggcctcgtg atcccaccag cccagctgtg ggtcgtgctc ggcgagggtg cggatgatgc
      421 gctccgcgaa cacgctgagc aggtcgcgca cgtggacgcc gtactccagg gcggaccagg
      481 tgaccggcgc cggccgctgg cgcacggcgt cgtcgcccgc ggcggcgagc agttcgccga
      541 gcgcgtggac ctcgtcgcgg acctgggcgc cgatggcccg ggcggcgagc tcgccgttga
      601 ccagcccgca cccggcgcag cgccggccgt cggcgacgat gccgggccag ctctcctggc
      661 cggtgccggc cccgccatcg gtccgcgagc cggccgcgtc gggcgcagga ccggaaggaa
      721 cctcctgttc gggcggggcg ggcgtggcgg gcgcgcccgc cgccgagtcg agcgcatcgg
      781 cggggactgc cggggccggc gcagcctcga cggccggggc atcgggcggg aggtagccgt
      841 cggccggccc gccggggatc accgccccga tgcgccagag gttgccgtcg aggtcctcgg
      901 tggcgaactc ccggatgccg tagggctggt cctcgggcgg ggcgacggtg ggcgctccca
      961 tcgcggtcca caccgcgaac acggcatcgg cgtcggccac ccggaggtag gcgcccgaca
     1021 ccgactcggt ggcgggcggg aggccgggca gctcgttcag gtgcagttcg gcgccgtcga
     1081 gaccgaggat ggcgtagccg tcgaagaagg cccggacccg gaaccccagc cgctcgtacc
     1141 aggccacggc acggaccagg tcggccaccg gcaggacggg aacggaggcg gcggcagcgg
     1201 tcacgaccgg ccgaagaggt ggtcgcccac cagcgccagg ccggtgaggt cgtggacgtc
     1261 ggtgcgcagg aacgggaccc ggatcaccgg cgctggctcc accatctggg ccaggccggc
     1321 gaggtgctcc tcctcgcggt gggcgacgag cgagaagtcg gccaggttcc ggtagagccc
     1381 gcccaggtcc gatccggcga acgtctcgga ccgctcgcgc agggcctcgg gcagctcgtc
     1441 ggtgaagtgc gggtgcatgc ggttcacgac gagggcggcg atggggatgt cggcctcgcc
     1501 cagcttctcg gcgaagaacc gggcctcggc cacggtgtcg cgcttgggcg aggcgacgag
     1561 gacgtaggac gtggcgggac tggtgaggag ctggagcacg tgttcggccc ggtccttgaa
     1621 cccggcctcc atgccgtcga acgcctggaa gaaggtgacg gcatcctcga agaccgaagc
     1681 gcccaccacc ttcgacacct ggcgcacgaa ggcctgggcg gcgacgttga ccgccttcat
     1741 cacgccacga gtgggcgcca tgatcacccg gtagaggcgg tggtcgagga agcgggtcag
     1801 gcgcttgggg gcatcgagga agtccagggc gttgcggctc ggcggggtgt cgacgacgac
     1861 gaggtcgaag tcggtgctgt tgtgcagctc gtagagcttc tccatcgcca tgtactcctg
     1921 ggtgcccgag agggcgccgg agatgttctt gtagaagcgg ttggcgagga tgcgctcggc
     1981 ctgctcgggc tcggccgagt acgtggtgac gaggtcgtcg aacgtgctct tggtgtcgag
     2041 catcacggcc cacagctcgc cgggccaggg tccgtcgatc cgcttcgggg tgttgccgat
     2101 gccgtcgagg ccgagcgcgt cggccagccg cttggccgga tcgatcgtga cgacgacggc
     2161 acgccggccg acgttggccg cctccatcgc cacgaccgcg gcggtggtgg tcttgcccac
     2221 gccgccggag ccggagcaga tgatgatgcg gctggtggtc accaggtcgg tgaggcctgc
     2281 ggctccggtc gcggcgtcgg tcacgacagg cccccgatcc cgtcgagcat cgatgccgcc
     2341 agctgttcga gctgcgccgg gccgagctcg gcgtcgaaca ggtaggggag cgacagctgg
     2401 gggagcggga gcgcctcggc gagccggccg acctgctcgg cctgcagctc catccgctcg
     2461 cggcggaacg tggccgcggc gtcgagcgcg tcggcctctc cggggcgcag gctcgcgccg
     2521 gctgcctcag cggcggccga cggatcggca tcgagaccgg tgaggggcgg gtacagcccg
     2581 ttgaccacca ccggaccgag gctgatgccc acccggtcct ccagttggta cgccgtctcg
     2641 accagctcgt tgaccggtgt ctcctcgggc agggtcacga gcatgacctg gcaccgctcg
     2701 gggtcgccga ggagggcgag gacgtctttg gcctggttgt tgatcgggcc gatgggcacg
     2761 gcgtcggcca ggccgctggc cgactggagg aagctgatgg cgtggccggc ggcgggcgcg
     2821 tcgaggatca ggaggtcggc gaggccggcc cgctccatct gcttgatctt ccccaagacg
     2881 aggatgtccc ggatgccggg gatggccgtg gacaccatgt cgagggcgcc gctcgtcatg
     2941 agccgcttcg agatgcggct catgtcggag tcttcgaggt actcgaccag ggcctcgtcg
     3001 ggggtgaggg tccg
//
LOCUS       DATMFS010000341         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_128302, whole genome shotgun sequence.
ACCESSION   DATMFS010000341 DATMFS010000000
VERSION     DATMFS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128302"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2661
                     /locus_tag="VNQ33_06210"
     CDS             <1..2661
                     /locus_tag="VNQ33_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWJ97735.1"
                     /translation="QGLIYEAYRVLPYSWGAETPLSNFEIRLDDATRPRQDPALTVAF
                     DLDPVDGDPGPLKILAWTTTPWTLPSNLALAVGPDLTYAIVELDGTRYVLGEAVLEHY
                     AAELGEATRVGTLTGADLVGRTYTPLFGYFADNPDSFRVLAGDFIDTTEGTGVVHIAP
                     GFGEDDQRIGNEAGIALVVPVDEKGQFTADVSDWEGVNVFEANPDIIRHLKAEGRVLR
                     HETYDHNYPHCWRTDTPIIYRAMSSWYVRVSDLTDKLLAHNQEINWIPEHVKDGSFGK
                     WLAGAHDWSISRNRFWGSPIPIWQSDDPAYPRTDVYGSLDEIEADFGVRPTDLHRPFI
                     DDLTRPNPDDPTGTSTMRRVPEVLDCWFDSGSMPYAQVHYPFENKEWFDAHAPADYIV
                     EYIAQTRGWFYTLHVLSVALFDRPAFSNVNCHGVVLDAEGRKLSKKLRNYPDPEEVME
                     EIGSDALRWYLMSSPILRGGDLKIAEDGSGITDMVRLVLNPIWNAFHFFTLYANADGY
                     RAQFRTDATGELDRYLLAKTRTLVEAVTVAMDAYDIAGACNQITSYLEALNNWYIRSS
                     RERFWAPGGVDAAAEGAAAEKADAFDTLYTVLVTLSKVVAPLLPMLAEEIHTGLTGER
                     SVHLADWPEASALPADAELVQAMDEVRAVCSTALGLREERKLRSRLPLPSLAVAGGRA
                     AALRPFTHLIARELNVKAVEISDDRTAYGTEVVRPNPRVLGPRLGKQVQEVIKAAKSG
                     DWSRSDDGAVVVGGHPLTDGEYELVLQTAEDVTAAPVRSVDPEGRTIDTGLVVALDTN
                     VTPELHAEGVTRDLVRAVQSARKDAGLDVTDRISLALQLPAEQRAMVEANQQHLADSV
                     LAASVAYVDVAQPSSCLLDGTEVTFAVTRA"
     gene            complement(2788..>3278)
                     /locus_tag="VNQ33_06215"
     CDS             complement(2788..>3278)
                     /locus_tag="VNQ33_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97736.1"
                     /translation="GPASAYLPARVDLDGPRPLRNIDLPATGTFAWGYSTDRSIRYDL
                     FAVVDADEYGSSDRAIATAVLRQLNGEPHAVEMHDIDERDGHVVGTYDERFDGRDYRM
                     HFVSAGGSVVALGVGVYLGSSRDTVEQAKADLEEMWGSLSVAGIDGVSKGTCTGPSDE
                     PG"
BASE COUNT          498 a         1269 c         1046 g          465 t
ORIGIN      
        1 cagggcctca tctacgaggc gtaccgggtg ctgccctaca gctggggcgc cgagaccccg
       61 ctgtcgaact tcgagatccg cctcgacgac gccacccggc cccgccagga cccggcgctc
      121 accgtggcgt tcgacctgga ccccgtcgac ggcgatcccg ggccgctcaa gatcctggcg
      181 tggaccacca cgccgtggac cctgccgtcc aacctggccc tggccgtcgg gcccgacctc
      241 acctacgcca tcgtcgagct cgacggcacg cgctacgtgc tgggcgaggc cgtgctcgag
      301 cactacgccg ccgagctggg ggaggccacc cgggtcggca cgctcaccgg cgccgacctg
      361 gtgggccgca cctacacccc gctgttcggc tacttcgccg acaaccccga cagcttccgg
      421 gtgctggccg gcgacttcat cgacaccacc gagggcaccg gcgtcgtgca catcgccccc
      481 ggcttcggcg aggacgacca gcgcatcggc aacgaggccg gcatcgcgct cgtggtgccc
      541 gtcgacgaga agggccagtt caccgccgac gtctcggact gggagggcgt caacgtcttc
      601 gaggccaacc cggacatcat ccggcacctc aaggccgagg gccgggtgct ccggcacgag
      661 acctacgacc acaactaccc gcactgctgg cgcaccgaca cgccgatcat ctaccgggcg
      721 atgtcgtctt ggtacgtgcg cgtcagcgac ctcaccgaca agctcctggc ccacaaccag
      781 gagatcaact ggatccccga acacgtcaag gacggctcct tcggcaagtg gctggccggc
      841 gctcacgact ggtccatcag ccgcaaccgg ttctggggat cgccgatccc gatctggcag
      901 agcgacgacc cggcgtaccc gcgcaccgac gtgtacggca gcctcgacga gatcgaagcc
      961 gacttcggcg tccggcccac cgacctgcac cggccgttca tcgacgacct cacccggccc
     1021 aaccccgacg atcccaccgg gacgtcgacc atgcgccgcg tccccgaggt cctcgactgc
     1081 tggttcgact cggggtccat gccctacgcg caggtgcact accccttcga gaacaaggag
     1141 tggttcgacg cccacgcgcc cgcggactac atcgtcgagt acatcgccca gacgcgcggc
     1201 tggttctaca cgctgcacgt cttgtcggtc gccctgttcg accgcccggc gttctccaac
     1261 gtgaactgcc acggcgtggt cctcgatgcc gagggccgca agctctccaa gaagctccgc
     1321 aactaccccg atcccgagga ggtcatggag gagatcgggt ccgacgcgct gcgctggtac
     1381 ctcatgtcct cgccgatcct gcgcggcggc gacctcaaga tcgccgagga cggctcgggc
     1441 atcaccgaca tggtgcgcct ggtcctcaac ccgatctgga acgccttcca cttcttcacg
     1501 ctctacgcca acgccgacgg ctaccgggcg cagttccgca ccgacgccac cggcgagctg
     1561 gaccgctacc tgctggccaa gacccgcacc ttggtcgagg cggtcaccgt ggccatggac
     1621 gcgtacgaca tcgccggcgc gtgcaaccag atcacctcgt acctcgaggc gctcaacaac
     1681 tggtacatcc gctccagccg cgagcgcttc tgggcacccg gtggcgtcga tgccgcagct
     1741 gagggcgcgg cggccgagaa ggccgacgcg ttcgacacgc tctacacggt gctcgtcacc
     1801 ctctcgaagg tggtggcccc gctgctgccg atgctggccg aggagatcca caccgggctc
     1861 accggcgagc gctcggtgca cctggccgac tggcccgaag cctccgcgct cccggccgat
     1921 gccgaactgg tgcaggccat ggacgaggtc cgcgccgtgt gctccaccgc cctcggcctg
     1981 cgtgaggagc gcaagctgcg cagccgcctc cccctcccct cgctcgcggt ggccggcggg
     2041 cgagcggctg ccctccggcc cttcacgcac ctcatcgccc gcgagctcaa cgtcaaggcc
     2101 gtcgagatct ccgacgaccg caccgcctac ggcaccgagg tcgtgcggcc caacccgcgc
     2161 gtgctcggtc cccggttggg caagcaggtc caggaggtca tcaaggcggc caagagcggc
     2221 gactggtccc gctccgacga tggcgccgtc gtggtcggtg ggcacccgct caccgacggc
     2281 gagtacgagc tcgtgctgca gacggccgag gacgtcaccg ccgcgccggt gcgctccgtc
     2341 gaccccgagg gccgcaccat cgacaccggc ctggtcgtcg cgctcgacac gaacgtcacg
     2401 ccggagctcc atgccgaggg cgtcacccgg gacctcgtcc gggccgtcca gtccgctcgg
     2461 aaggacgccg gcctcgacgt caccgaccgc atctcgctcg ccctccagct ccctgccgag
     2521 cagcgggcga tggtggaggc caaccagcag cacctggccg acagcgtgct ggccgcctcg
     2581 gtcgcctacg tcgacgtggc ccagccctcg agctgcctcc tcgacggcac ggaggtgacc
     2641 ttcgccgtca cccgggcctg acgcgctccc gtccgggtca gcaccgccgt cgggctgagt
     2701 cccagcggcc caccagcccg cgccgctcgc cttcgcgcag aaccggccct gcaccgtgcc
     2761 gtcccagacc gtcctacctc gccgggatca gcccggttcg tcggacggac cggtgcaggt
     2821 ccccttcgac acgccgtcga tgccggcgac cgacagcgag ccccacatct cctcgaggtc
     2881 ggctttggcc tgctccaccg tgtctcgact gctgccgagg tagacgccga cgccgagggc
     2941 gacgacggag ccgccggccg acacgaagtg catgcggtag tcgcgcccgt cgaagcgctc
     3001 gtcgtaggtc ccgacgacgt ggccgtcgcg ctcgtcgatg tcgtgcatct ccaccgcgtg
     3061 cggctcgccg ttgagctggc ggagcaccgc tgtggcgatc gctcggtcgc tgctgccgta
     3121 ctcgtcggca tccacgaccg cgaacaggtc gtaccggatg gagcggtcgg tgctgtagcc
     3181 ccaggcgaag gtgcccgtcg ccgggaggtc gatgttgcgc agcggccgcg ggccgtcgag
     3241 gtcgacccga gccggcaggt acgccgaagc cggcccct
//
LOCUS       DATMFS010000342         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_314755, whole genome shotgun sequence.
ACCESSION   DATMFS010000342 DATMFS010000000
VERSION     DATMFS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314755"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VNQ33_06220"
     CDS             complement(<1..2138)
                     /locus_tag="VNQ33_06220"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HWJ97737.1"
                     /translation="MGPVLSMRVKAMAVTATVVASSVAIVFAADAPSAGAEGKSQAYW
                     EQFLEDEQLQNDIASRIVMTDFDPTDAAHPSAVAFSTTLETGQCNAQQRRVAAPSMTE
                     YPLYVFDLTGEDTVKRPIFWTGSQECNTVVDLDMTPDGRTIVAQSTRISENEIDGCPG
                     LYYNRTQIISWTRSAVGEEFGQPHLVSVADDQPEECSPEVENPMGGPEHLLGVGGDSG
                     SSSPSISADGAVVAFTSTADNFGVGPTAASTAQALFVAGPDGDDLSMVTPASFDGVAS
                     DAEISGDGNSIVFTAVGSGLDPAVTAADGNQVFLATRDSGALTGWRYDLISGKRSDPE
                     DAGTYGPLDGNSTAPRISEDGTRVVFTTSASDIEDGVITDDGDTDYWLVAREVSDLNA
                     HPVRVILNPDDGTHGYPTSNGLGEPELSADGQRVAITADNDGKSRFRMFDVAMALRDD
                     ISPAPPGLRSQAAIKGAGRVIAGSAYRPLGLGGRGDKSLAALGGMPPFDRYRGSGAAL
                     YLGGGGEIGPDISKGLHGDPVDTANGAFIHHETDFTAAYAAAFMAVPRSYSSVGDQGG
                     LFGAGWASPLDTSLDVTDELGTDVVLRLPSGRGVIYTESGGSWSTTMGYRSQLAARSG
                     GGWEVTDPNGDVWTFDEDGLLTAWSSAAGSVEIGARSAEGVPETITADNGDVLALSDT
                     TSVLSNGSTVNDPDGLVDRITAPHGIVVDYS"
BASE COUNT          344 a          817 c          732 g          366 t
ORIGIN      
        1 gagtagtcga ccacgatgcc gtggggtgcg gtgatgcggt cgaccagacc gtcggggtcg
       61 ttcacggtgc tgccgttgga gaggacgctg gtcgtgtccg acagggcgag gacatcgccg
      121 ttgtcggcgg tgatcgtctc gggaacgcct tcggccgagc gggccccgat ctcgaccgac
      181 cccgcagctg atgaccacgc ggtgaggaga ccatcctcgt cgaaggtcca cacgtcgccg
      241 ttcgggtcgg tcacctccca gcccccaccc gatctcgcag cgagctggga ccggtagccc
      301 atcgtcgtgg accaggagcc gcccgactcg gtgtagatca caccgcggcc gctcggcagc
      361 cgcaggacca cgtcagtccc cagctcatcg gtgacatcga ggctggtgtc gagcggtgac
      421 gcccatccgg ccccgaacag gccgccttgg tcgccgaccg agctgtacga gcgcggcact
      481 gccatgaacg ccgccgcgta ggccgcggtg aagtccgtct cgtggtggat gaacgccccg
      541 ttggcggtgt ccacgggatc gccgtggagg cccttgctga tgtcggggcc gatctcaccg
      601 ccaccgccca ggtacagcgc agctcccgag cctctgtagc gatcgaacgg gggcatgccg
      661 ccgagcgcgg cgaggctctt gtcgccacgg ccgcccagtc cgagagggcg gtaggcactg
      721 cctgcgatca cccgccccgc gcccttgatc gcggcttgcg agcggaggcc cgggggcgcc
      781 ggcgagatgt cgtcgcgcag cgccatggcc acatcgaaca tccggaaccg cgacttgccg
      841 tcgttgtccg cggtgatcgc cacccgctgc ccgtctgccg acagctccgg ttcacccagc
      901 ccgttcgacg tcggatagcc gtgggtgccg tcgtccgggt tcaagatcac gcggaccgga
      961 tgcgcgttca ggtccgagac ctcgcgggca accagccagt agtcggtgtc accgtcgtcg
     1021 gtgatgaccc cgtcttcgat gtcgctcgcc gatgtcgtga acaccacccg ggtcccgtcc
     1081 tccgagatcc gaggagcggt gctgttcccg tcgagcggac cgtacgtacc ggcatcctca
     1141 ggatccgagc gcttccccga gatcaagtcg tatctccacc cggtcagggc gcccgagtcc
     1201 cgggtggcca ggaagacctg gttgccatcg gccgcggtga ccgccgggtc gagaccggac
     1261 cccacagcgg tgaacacgat cgagttccca tcaccggaga tctcggcgtc gctcgccacc
     1321 ccgtcgaacg acgccggggt caccatcgag aggtcgtcgc cgtcgggacc ggcgacgaac
     1381 agcgcctgcg ccgtcgaggc cgcggtgggg ccgacaccga agttgtccgc cgtcgacgtg
     1441 aacgccacga cggcaccgtc agcggagatc gacggcgacg agctcccgct gtcgccgccc
     1501 accccgagca ggtgctccgg tccgcccatg gggttctcga cctccggcga gcattcctcc
     1561 ggctggtcgt ccgccaccga gacgaggtgc ggctgaccga actcctcgcc gaccgcggaa
     1621 cgggtccagc tgatgatctg cgtccggttg tagtagaggc ccgggcaacc atcgatctcg
     1681 ttctcggaga tccgagtgct ctgggcgacg atggtcctgc cgtcaggcgt catgtccagg
     1741 tccacgaccg tgttgcactc ctggctgccc gtccagaaga tgggccgctt caccgtgtct
     1801 tcgccggtga ggtcgaagac gtagagcggg tactccgtca tcgaaggagc cgccacccga
     1861 cgctgctgcg cattgcactg tcctgtctcc aacgtcgtcg agaacgccac cgccgacggg
     1921 tgggccgcat cggtgggatc gaaatcggtc atcacgatcc gcgacgcgat gtcgttctga
     1981 agctgttcat cctcgaggaa ctgctcccag tacgcctgcg acttcccctc ggcaccagcc
     2041 gacggtgcat cggccgcgaa cacgatggcg accgacgacg ccaccaccgt cgcggtcacc
     2101 gccatggcct tcacccgcat cgacagcacc ggccccatca atcttctccc cgctcgatcc
     2161 cgcacgtctc gtagatctcc tcgaccggtg ccgtcgccga cgcctccgcc ttctccgggt
     2221 tcagcaccgc gcccgccgcc gcctcgatct gctcgggtg
//
LOCUS       DATMFS010000343         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_265758, whole genome shotgun sequence.
ACCESSION   DATMFS010000343 DATMFS010000000
VERSION     DATMFS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265758"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VNQ33_06225"
     CDS             <1..493
                     /locus_tag="VNQ33_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWJ97738.1"
                     /translation="EGLRVLSNRGANGIDGVVSTAVGAALAARGTGAPTVVLIGDVAF
                     LHDSNALLGLADRGVDLTIVVVDNDGGGIFSFLPQGGALGSERFEQLFGTPHGVDLSV
                     LAAAHGLMTVEPATGADVADAVLASVGAGGARVVQIRTDRVINVQVHDELHAAVAAAL
                     DAD"
     gene            complement(497..925)
                     /locus_tag="VNQ33_06230"
     CDS             complement(497..925)
                     /locus_tag="VNQ33_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97739.1"
                     /translation="MTISVRRLGPDDAEVVLAAGHLFDAAPNPGWTADVLSRPGHHLL
                     FAFEDEVPVGFATCIEMAHPDKGTEVMIYELGVDEPHRRNGIGRLLVERSLDLARARG
                     CYGAWTVTEDDNAAALATYRSAGAEPDPTCVTQVWDWRAG"
     gene            complement(977..>2016)
                     /locus_tag="VNQ33_06235"
     CDS             complement(977..>2016)
                     /locus_tag="VNQ33_06235"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWJ97740.1"
                     /translation="DPVGHPGTGPIAFGALPFLPGGAAELLVPEVLVGVDAAGEAWAT
                     TVSAGGTDPIAAVEAVLAESRRPSPSDGPSSFTVSAARPPADWEAAVAAATARIRAGE
                     LDKVVLAREITVTADGPISIATVLLRLSQHYPGCYLYLVDGYCGATPELLVGRAGDVV
                     RAQPMAGTKPRRGDPDADAVLAAELLASATYRQEHQVTIDAVHDTLLAFSSYVDYEPE
                     PSVVPLANVVHLATQVEGRLSHPPASILELVGALHPTPAVCGRPRDAALAVIAELEEL
                     DRGRYAGAVGWVDADGNGDWAVAIRGAEIAGTTARVIAGNGMVGDSDPPTELIETRAK
                     LQAMLSAIVRP"
BASE COUNT          270 a          693 c          793 g          260 t
ORIGIN      
        1 cgagggcctg cgggtgctgt cgaaccgggg tgccaacggg atcgacggcg tcgtgtcgac
       61 cgcggtgggc gcggccctcg ccgcccgggg cacgggtgcc ccgaccgtgg tgctgatcgg
      121 cgacgtggcc ttcctgcacg actcgaacgc cctgctgggc ttggccgacc ggggcgtcga
      181 cctcacgatc gtcgtggtcg acaacgacgg cggcggcatc ttctcgttcc tgccccaggg
      241 cggcgccctc gggtccgagc gcttcgagca gctgttcggc acgccccacg gcgtcgacct
      301 gtcggtcctc gccgccgccc acggcctcat gaccgtcgag cccgcgacgg gcgccgacgt
      361 cgccgacgcg gtgctcgcaa gcgttggagc cggcggggca cgagtcgtcc agatccgcac
      421 cgaccgcgtc atcaacgtcc aggtccacga cgagctgcac gccgcggtgg ccgcagcact
      481 cgacgcggac tgaccctcag ccggcgcgcc agtcccagac ctgggtcacg caggtggggt
      541 cgggctcggc tcccgccgag cggtacgtcg cgagcgccgc ggcgttgtcg tcctcggtga
      601 cggtccaggc gccgtagcaa ccccgagccc gggcgagatc gagggaccgc tcgacgagca
      661 ggcgtccgat gccgttgcgt cggtgcggct cgtcgacgcc gagctcgtag atcatcacct
      721 cggtaccctt gtcgggatgc gccatctcga tgcaggtggc gaaccccacc ggaacctcgt
      781 cctcgaacgc gaagaggagg tggtggccag gccgcgagag cacgtccgcc gtccacccgg
      841 ggtttggagc cgcatcgaag aggtgcccag cggcaaggac aacctcggcg tcgtcgggac
      901 cgagccgccg cacggagatc gtcatggtcg ccatgctcgc gcggcgactc gtggacgaag
      961 ccggccattc ggcggcttag ggccgcacga tggcgctcag catggcttgg agcttggctc
     1021 tcgtctcgat gagctcggtg ggcgggtcgg agtcgccgac catgccgttg ccggcgatga
     1081 cgcgggcggt ggtgccggcg atctcggcgc cgcggatggc gacggcccag tcgccgttgc
     1141 cgtcggcgtc gacccagccg acggcgccgg cgtagcggcc ccggtccagc tcctcgagct
     1201 cggcgatgac ggccagcgcc gcgtcgcggg gacggccgca cacggcaggg gtcgggtgca
     1261 gcgcgccgac cagttcgagg atcgacgcgg gcgggtgcga caggcgtccc tcgacctggg
     1321 tggcgaggtg cacgacgttg gcgagcggca cgaccgacgg ctcgggctcg tagtccacgt
     1381 aggaggagaa ggcgagcagc gtgtcgtgca cggcgtcgat cgtgacctgg tgctcctggc
     1441 ggtaggtggc cgacgccagc agctcggcgg cgagcacggc gtcggcatcg gggtcgcccc
     1501 gccggggctt ggtgccggcc atgggctggg cgcggacgac gtcgccggca cggccgacga
     1561 gcagctcggg ggtggccccg cagtacccgt cgaccaggta caggtagcag ccggggtagt
     1621 gctgcgacag gcggagcagg acggtcgcga tggagatggg gccatcggcc gtgacggtga
     1681 tctcacgggc gagcaccacc ttgtcgagct caccggcgcg gatgcgtgcc gtggcggcag
     1741 cgacggcggc ctcccagtcg gccggcgggc gggccgcgga gacggtgaac gacgacgggc
     1801 cgtcggaggg ggagggccgg cgcgactcgg cgagcaccgc ctcgaccgcg gcgatggggt
     1861 cggtgccgcc cgcgctcacg gtcgtggccc aggcctcgcc agcagcgtcg acgccgacga
     1921 gcacctcggg cacgaggagc tcggcggcac caccggggag gaaggggagc gcgccgaagg
     1981 cgatcgggcc cgtgccgggg tggcccaccg ggtcgt
//
LOCUS       DATMFS010000344         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_35687, whole genome shotgun sequence.
ACCESSION   DATMFS010000344 DATMFS010000000
VERSION     DATMFS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35687"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1019
                     /locus_tag="VNQ33_06240"
     CDS             192..1019
                     /locus_tag="VNQ33_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HWJ97741.1"
                     /translation="MAEPTRPPLSAPASARPMVFVALCAVTVLVVAWALGNGGDGGTE
                     QAAEKAAAAATSTLPVRIPTAAAPLKVLVAGDSLVGWIAPALEQELPAGDPVEVIDDW
                     KGSTGLVRTDYFDWPAKLTADMAQHDPDVVIVGFGGNDTQGFTTDAGVIALGTPEWAD
                     EYQRRVGEVLDIIEEPGRTVYWIGMPLTRSEAIEALRPVLTEAVEAELASRPWAHFVD
                     PRDALAPDGTFVSERPGDDGRLVTTMAPDGIHPSLDGGVFIVRAFLPDIRAERQLAP"
     gene            1120..2463
                     /locus_tag="VNQ33_06245"
     CDS             1120..2463
                     /locus_tag="VNQ33_06245"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWJ97742.1"
                     /translation="MSDAPPSAAYADDRAHVFHSWSAQKLLDPLVIVGGEGSWIWTED
                     GTRYLDFSSQLMNVNIGHQHPKLVAAIKEQADTLATIAPFHATPARSEAARLIAEKAG
                     GHFEMVFFTNGGAEANENAIRMARLHTGRHKVLAAYRSYHGATHGAISLTGDPRRWPS
                     EPAIPGTVHFWGPYPYRSAFDSGSPEQETERALQHLADTLMVEGPQTVAAVILEPVVG
                     TNGILVPPPGYLAGVRELCDQHGILLIADEVMAGFGRCGEWFSIQHWDVQPDLICFAK
                     GVNSGYVPLGGVVISAGIAATFAERPYPGGLTYSGHPLACASAVASIRIFEEEGLVER
                     ARRMGDEVAGPALRKLAETHPSVGDVRGLGLFWALELVKDRETREQLVPFNAAGPDAG
                     PVTEVVAACKKQGLLVFAHFNRIHVVPPLTISEDDFAKGVAILDGALSLADAHTA"
     gene            complement(2677..>3359)
                     /locus_tag="VNQ33_06250"
     CDS             complement(2677..>3359)
                     /locus_tag="VNQ33_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ97743.1"
                     /translation="VSAAYGSAGERCMAVSVVLAMDPVADALIGKITERIPAIKVGPG
                     TEPDSEMGPLITGEHRDKVAGYVAGASTEGATVVVDGTADVPAGDGFFLKPTLLDGVQ
                     PGMACYDDEIFGPVLSVTRIGSYDEGLQLINGNPFGNGVALFTRDGGVARQFQFDVEV
                     GMVGINVPIPVPVSYYSFGGWKASLFGDQHMYGPEGISFFTRSKVVTSRWPDPATSTV
                     DLGFPENR"
BASE COUNT          482 a         1248 c         1158 g          471 t
ORIGIN      
        1 acctccaggt cgtcgtcatc gccgaacccg agggtccgcc acccagcatc gacgagggag
       61 ggcaggtcgt ggtcatcagc catccccgca cgctagcgat gcacgcgccg acggccggct
      121 gggctggggg ggtcggatcg accgccggag gtgcagcggg cggccgggtc cagcagactt
      181 ccggtctccc catggccgag cccacccgac cgcccctgag cgcccccgcc tcggcgcggc
      241 cgatggtgtt cgtcgcgctc tgcgccgtga cggtgctggt cgtcgcctgg gcgctcggca
      301 acggcggcga cggcgggacg gagcaggcgg cggagaaggc cgcggccgcc gccacctcga
      361 cgcttcccgt tcgcatcccg accgccgccg ccccgctcaa ggtgctcgtc gccggcgact
      421 cgctcgtggg ctggatcgca ccggccctgg agcaggagct cccggccggc gatccggtgg
      481 aggtcatcga cgactggaag ggctcgaccg gcctcgtccg caccgactac ttcgactggc
      541 ccgccaagct caccgccgac atggcgcagc acgaccccga cgtcgtcatc gtcggcttcg
      601 gcggcaacga cacccagggc ttcacgaccg atgcgggggt catcgccctc gggacccccg
      661 agtgggccga cgagtaccag cgccgcgtcg gcgaggtgct cgacatcatc gaggagccgg
      721 gccgcacggt ctactggatc ggcatgccgc tgacccggag cgaggccatc gaggcgctcc
      781 ggccggtgct gaccgaggcc gtcgaggccg agctcgcctc gcgcccgtgg gcgcacttcg
      841 tcgacccccg ggatgcgctc gcccccgacg ggaccttcgt gagcgaacgg cccggcgatg
      901 acggccggct cgtgaccacg atggcgcccg acggcatcca ccccagcctc gacggcggcg
      961 tcttcatcgt ccgggcgttc ctgccggaca tccgagcgga gcgccagctc gcgccgtgac
     1021 catcgaccga accgcgtgcg gcatttgtac caggtacaaa catggttctg ttctccatcg
     1081 agcccgacga tcggagcagg tggcgtatcg ttgacgccca tgagcgatgc ccctccctcc
     1141 gccgcgtacg ccgacgaccg ggcccacgtc ttccactcgt ggtcggccca gaagctgctc
     1201 gaccccctgg tgatcgtggg cggcgagggc tcgtggatct ggaccgagga cggcacccgc
     1261 tacctcgact tctcgagcca gctgatgaac gtgaacatcg gccaccagca ccccaagctc
     1321 gtcgccgcca tcaaggagca ggccgacacc ctggccacca tcgccccgtt ccacgccacg
     1381 cccgcacgca gcgaggccgc ccggctgatc gccgagaagg ccggcggcca cttcgagatg
     1441 gtgttcttca ccaacggcgg cgccgaggcc aacgagaacg ccatccgcat ggcccgcctg
     1501 cacaccggcc gccacaaggt gctcgccgcc taccgcagct accacggcgc cacccacggg
     1561 gccatcagcc tcaccggcga tccccgccgc tggccgtcgg agccggccat cccgggcacc
     1621 gtccacttct ggggcccgta cccctaccgc tcggcgttcg acagcggctc gccggagcag
     1681 gagaccgagc gggcgctcca gcacctggcc gacaccctga tggtcgaggg cccgcagacc
     1741 gtcgccgccg tcatcctcga gccggtggtc ggcaccaacg gcatcctcgt gccgccgccc
     1801 gggtacctgg ccggcgtccg cgagctgtgc gaccagcacg ggatcctcct catcgccgat
     1861 gaggtcatgg ccggcttcgg ccgctgcggc gagtggttct cgatccagca ctgggacgtg
     1921 cagcccgacc tcatctgctt cgccaagggc gtcaacagcg ggtacgtccc gctcggcggt
     1981 gtcgtgatct ccgccggcat cgccgccacc ttcgcggagc ggccctaccc cggcggcctc
     2041 acctacagcg gccacccgct cgcttgcgcc tcggccgtgg cgtcgatccg catcttcgag
     2101 gaggagggcc tcgtcgagcg ggcccggcgc atgggcgacg aggtcgccgg cccggcgctg
     2161 cggaagctgg ccgagaccca cccgtccgtc ggcgacgtcc ggggcctggg cctgttctgg
     2221 gcgctcgagc tggtcaagga ccgcgagacc cgcgagcagc tggtgccgtt caacgccgcc
     2281 ggccccgacg ctggcccggt taccgaggtc gtggccgcgt gcaagaagca gggcctcttg
     2341 gtcttcgccc acttcaaccg catccacgtg gtccccccgc tcaccatcag cgaggacgac
     2401 ttcgcgaagg gcgtcgccat cctcgacggc gccctgtccc tggccgacgc ccacacggcc
     2461 tgactcggcg gaggcggtac ccggtccgcg gcgagcgtct ccgccgtcat ctgtcgctgc
     2521 tcgtggactc gggtgccacg cagcgtgggg cgaatcgggc atcaccggcg atccagctga
     2581 tttcgggtca cctgtgcacc gtgggcgtgc acaggtgacc cgaaatccga ggtgcgcccg
     2641 gaaccggggc cgggggaggg agtcccgggc ttcgagctag cggttctcgg ggaagccgag
     2701 gtcgacggtg ctggtggcgg ggtcgggcca gcgactggtg accaccttgg agcgggtgaa
     2761 gaagctgatg ccctcggggc cgtacatgtg ctggtcgccg aagagcgagg ccttccagcc
     2821 gccgaagctg tagtagctga cgggcaccgg gatcgggacg ttgatgccga ccatgccgac
     2881 ctcgacgtcg aactggaact gccgggccac gccgccgtcg cgggtgaaca gggcgacgcc
     2941 gttgccgaac gggttgccgt tgatgagctg caggccctcg tcgtaggagc cgatgcgggt
     3001 gaccgacagc accgggccga agatctcgtc gtcgtagcag gccatccccg gctgcacgcc
     3061 gtcgagcagc gtcggcttga ggaagaaccc gtcgccggcg ggaacgtcgg cggtgccgtc
     3121 gacgacgacg gtcgcgccct cggtcgacgc gccggcgacg tacccggcga ccttgtcgcg
     3181 gtgctcgccg gtgatgagcg ggcccatctc ggaatcgggc tcggtgcccg ggccgacctt
     3241 gatggcgggg atgcgctcgg tgatcttgcc gatgagggca tcggccaccg ggtccatggc
     3301 caagacgacc gacacggcca tgcaccgctc tcccgccgat ccgtaggccg cgctcaccg
//
LOCUS       DATMFS010000345         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_12500, whole genome shotgun sequence.
ACCESSION   DATMFS010000345 DATMFS010000000
VERSION     DATMFS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12500"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..965)
                     /gene="proC"
                     /locus_tag="VNQ33_06255"
     CDS             complement(183..965)
                     /gene="proC"
                     /locus_tag="VNQ33_06255"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWJ97744.1"
                     /translation="MTKLLVVGGGKMGEALLGGLLAGRWAAPEEVAVVEPVAARRAEL
                     AERYPGVRLTSGTVSADGAVLAVKPSMIPDLCAELGELGIRRLLSIAAGVRLATMEAV
                     VPPGTAVVRAMPNTPALVGMGASAIAGGAAATDDDLDWAETILGAVGTVTRVSEAQIN
                     MVTGLSGSGPAYVFLIAEALIDAGVLVGLPREVASDLTVQTLRGSAELLAAGTPPAEA
                     RANVTSPGGTTAAGLRALEDGKVRASIIDAVVAAKARADEMG"
     gene            complement(1169..1633)
                     /locus_tag="VNQ33_06260"
     CDS             complement(1169..1633)
                     /locus_tag="VNQ33_06260"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWJ97745.1"
                     /translation="MSDLAPDDELDAIESRIDAWAERELAGNPVVEAVERAPRSEGRR
                     WFLRIRGEEKDVFTVWLTLRQRTLHYETYVMPAPEENHGQLYEHLLRRNHGLTGMAFA
                     IGIEDAVFLQGSLPVHAVTDDELDRIFGSFYAYVEQCFRPALRIGFASRFRS"
     gene            complement(1630..2841)
                     /locus_tag="VNQ33_06265"
     CDS             complement(1630..2841)
                     /locus_tag="VNQ33_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ97746.1"
                     /translation="MTELAVLSLHSSPLTQPGTGDSGGMNVYVRELAASLAQSGVGTT
                     VYVRSWDPALPREVAVEPGFRVVHVPAGPADLAKEQLPETVDAFTEGVRRELAQRPVD
                     VIHANYWLSGVAGHRLKHQLDLPLVSTFHTLARVKAETGDPEPQRRIDAEAEVIGCSD
                     VILANSVEEVAQLETLYGADPTRIEVVPPGVEHAFFSPGDRAGARHALGLGDHPVLLF
                     VGRIQPLKGLPVAVGALAELHRPDAELVVVGGPSGADGHRELQAVLDLADHLGVRHQI
                     RFVAPQPHHLLSTSYRAADVVLVPSRSESFGLVALEAAACGIPVVAAAVGGLRTLIDH
                     GRTGFLVEQRDPPAFAAAVRSLLADPAAAARMGADAADRAQRYTWSTAAARLRRVYAD
                     LTLRSLVDCAA"
     gene            complement(2858..3355)
                     /locus_tag="VNQ33_06270"
     CDS             complement(2858..3355)
                     /locus_tag="VNQ33_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ97747.1"
                     /translation="MAELEARWHDLGEFIREQRKVGHLSLRKLSDMAGISNPYLSQIE
                     RGLRKPSAEILQQIARALEISSETLYIRAGILEPREGDTDLITEIRRDIWLNEEQKKT
                     LVQIYESFRAERRAAGFEPELVGQGSFDEEGADVSPPGPLAVVAGPGDTDDTDDPESE
                     ALAGR"
     gene            complement(3547..>3959)
                     /locus_tag="VNQ33_06275"
     CDS             complement(3547..>3959)
                     /locus_tag="VNQ33_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97748.1"
                     /translation="RLVQGRHDIVTGEIDRRSHGGDRYDVSDLVDRLPEILADRIHAP
                     GPGRLTPNMEPGDFSGSLADRLTEISERVPIETPAEADDEALAQVAADLSELEHEVSG
                     FRRQLFDRIDGLQAEITRRYRTGEAHVDDVLSGG"
BASE COUNT          562 a         1383 c         1497 g          517 t
ORIGIN      
        1 gctgcgcatc gggaccggcg acgaggtcgt gctcgcggcc atcggcgacg agccgccgcg
       61 ggcgggcgcc atcacccccg tcaccctgaa ggagaagcca tgaggcccgc caccaccatc
      121 gtgctcgccg tgctgctggg catgatcctg ctcgccgcca ccctccagct cgtggtgctg
      181 agctagccca tctcgtcggc gcgggccttg gcggcgacga cggcgtcgat gatcgaggcc
      241 cgcaccttgc cgtcctcgag cgcacggagc ccagccgccg tggttcctcc gggtgaggtg
      301 acgttggcgc gggcctccgc gggcggcgtg cccgccgcga gcagctcggc cgacccgcgc
      361 agcgtctgga ccgtcaggtc cgacgccacc tcgcgaggca acccgacgag gacgcccgcg
      421 tcgatgaggg cttcggcgat gaggaagacg taggccgggc ccgaccccga caggccggtg
      481 accatgttga tctgcgcctc gctgacgcgg gtgaccgtgc cgaccgcacc caggatcgtc
      541 tcggcccagt ccaggtcgtc gtcggtagcc gccgccccgc cggcgatggc cgacgccccc
      601 atgcccacga gggccggggt gttgggcatg gcccggacga ccgccgtacc cggtggcacc
      661 accgcctcca tcgtcgcgag ccggacgccg gccgcgatgg acaggagccg ccggatgccc
      721 agctcgccga gctccgcaca gagatccggg atcatcgaag gcttcaccgc cagcacggcg
      781 ccgtcggcgg acaccgtgcc gctggtcaac cgcaccccgg ggtagcgctc cgccagctcg
      841 gcgcggcgcg ccgcgacggg ttcgaccacc gccacctcct cgggagcggc ccatctgccg
      901 gcgagcaggc cgccgagcag cgcctcgccc atcttcccgc cgccgaccac caggagcttc
      961 gtcatggccg aaggctaggg cactgccgtc gccgtcccac cgggagggac gggttgcagc
     1021 cgacccgggg ggagggggag gggccggctg caacccgacg agcccggttg cgggtgcacc
     1081 ttccctcgat gccacccgtt ccgccgggag gcatcacctt accgaaaggg gtggaaagat
     1141 gtcaacagat gtggaggtga cgttcgtgtc agctccggaa ccgggacgcg aagccgatcc
     1201 gcagcgcggg gcggaagcac tgctcgacgt aggcgtagaa ggagccgaag atgcggtcga
     1261 gctcgtcgtc ggtgacggcg tgcaccggca gcgagccctg caggaacacc gcgtcctcga
     1321 tgccgatggc gaacgccatc ccggtgaggc cgtggttgcg ccgcagcagg tgctcgtaga
     1381 gttgcccgtg gttctcctcg ggcgccggca tcacgtaggt ctcgtagtgc agcgtgcgct
     1441 gccggagggt cagccagacg gtgaagacgt ccttctcctc gccgcggatc cgcaggaacc
     1501 agcgacggcc ctccgaccgg ggcgcccgct cgaccgcctc cacgacgggg ttgccggcca
     1561 gctcgcgctc ggcccaggca tcgatgcgcg actcgatggc gtcgagctcg tcgtcgggtg
     1621 cgaggtcgct catgcggcgc agtcgaccag cgagcgcagg gtcaagtcgg cgtagacccg
     1681 gcggaggcgg gcggcggccg tggaccaggt gtagcgctgg gcccgatcgg cggcatcggc
     1741 ccccatgcgg gcggcggcgg cgggatcggc gagcagcgag cggaccgcgg cggcgaacgc
     1801 cggcggatcg cgctgctcga cgaggaaacc cgtgcggccg tggtcgatca gggtgcgcag
     1861 cccgccgacg gcagccgcca cgacggggat cccgcacgcc gccgcttcga gcgcgaccaa
     1921 gccgaacgac tccgaccggc tggggaccag caccacgtcg gcagcgcggt aggaggtgga
     1981 gagcaggtgg tggggctggg gcgcgacgaa gcggatctgg tggcggacgc ccaggtggtc
     2041 ggccaggtcg aggacggcct gcagctcgcg gtggccgtcg gcgccgctgg ggccgccgac
     2101 cacgaccagc tcggcgtcgg gccggtgcag ctcggcgagc gcgccgacgg ccacgggcag
     2161 gcccttgagg ggttggatgc ggccgacgaa cagcagcacg gggtggtcgc ccaggccgag
     2221 ggcgtggcgc gcgccggcac ggtcgccggg cgagaagaag gcgtgctcga caccaggagg
     2281 caccacctcg atccgggtcg ggtcggcgcc gtagagcgtc tcgagctggg ccacctcttc
     2341 gacggagttg gccaggatca cgtcggagca gccgatgacc tcggcctcgg cgtcgatgcg
     2401 gcgctggggc tccgggtcac ccgtctccgc cttcacccgg gcgagggtgt ggaaggtgga
     2461 caccagcggc aggtcgagct ggtgcttgag ccggtggccg gccacacccg acagccagta
     2521 gttggcgtgg atgacgtcga ccggacgctg ggccagctcc cggcggacgc cctcggtgaa
     2581 ggcgtcgacc gtctccggga gctgctcctt ggcgaggtcc gccgggccgg cgggcacgtg
     2641 gaccacccgg aacccgggct cgaccgccac ctcccgcggc agcgccggat cccagctccg
     2701 cacgtacacc gtggtgccga cgccggactg ggcgagcgag gccgccagct cccgcacgta
     2761 cacgttcatc ccgccgctgt cgccggtgcc cggctgggtg agcggcgacg agtgcagcga
     2821 gaggacggcg agctcggtca cggaaggcac ggaggggtca acgcccggcg agcgcctcag
     2881 attccgggtc gtcggtgtcg tcggtgtcgc cgggaccggc cacgacggcc agcgggccgg
     2941 gaggcgagac gtccgcgccc tcctcgtcga acgagccctg gccgaccagc tcgggctcga
     3001 agcccgccgc acgccgctcg gcacggaacg actcgtagat ctgcacgagg gtcttcttct
     3061 gctcctcgtt gagccagatg tcccggcgga tctcggtgat gaggtcggtg tcgccctcgc
     3121 gcggctcgag gatgccggcg cggatgtaca gcgtctccga gctgatctcg agcgcccggg
     3181 cgatctgctg gaggatctcg gccgagggct tgcgcaggcc gcgctcgatc tggctcaggt
     3241 acgggttgga gatgcccgcc atgtccgaga gcttgcgcag ggacaggtgg cccaccttcc
     3301 gctgctcacg gatgaactcc cccagatcgt gccaacgcgc ttcgagctcg gccatgggcc
     3361 aaggctacga ccccgcctgg cgcgaacccc aactggagca agcgcacgat tgcccgctgc
     3421 aagcgcgaac gccgccacgc tccgcacccg acgaggtggt ggacgaggtg gtggaggagt
     3481 ccgatccctg ggtggggacg gggccacggc ggccgggccc cgacggcccg gagaggcgga
     3541 acggcgtcat ccgccagaga ggacgtcgtc gacgtgggct tcgccggtgc ggtagcggcg
     3601 ggtgatctcg gcctggaggc cgtcgatgcg gtcgaagagc tggcgccgga agcccgacac
     3661 ctcgtgctcg agctcgctca ggtctgcggc gacctgggcc agcgcctcgt cgtcggcctc
     3721 ggcgggtgtc tcgatcggga cgcgctcgga gatctcggtg aggcgatcgg cgaggctccc
     3781 gctgaagtcg cccggttcca tgttcggcgt gaggcggccc ggaccgggcg cgtggatgcg
     3841 gtcggcgagg atctcgggca gccggtccac caggtcggac acgtcgtagc ggtcgccgcc
     3901 gtggctgcgt cggtcgatct cgccggtgac gatgtcatgg cggccttgca cgagccgac
//
LOCUS       DATMFS010000346         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_221060, whole genome shotgun sequence.
ACCESSION   DATMFS010000346 DATMFS010000000
VERSION     DATMFS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221060"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VNQ33_06280"
     CDS             complement(<1..574)
                     /locus_tag="VNQ33_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97749.1"
                     /translation="MTRRPVPRAGLAALGALVLLLVSASPASADAPRPTDYRSTVTDV
                     SPALPSGATVRVIGGDSLLELSLPKGHTAVIADYPTSGEAEPVPYLRFDADGTVRRNT
                     LAVATTANQARYGASSTVPDPGAAPRWETVATNGTYAWHDHRIHWMSPKAPRVVAADG
                     RVDLGGDDGTWSVPLEVDGKDTVVTGELVLL"
     gene            complement(571..720)
                     /locus_tag="VNQ33_06285"
     CDS             complement(571..720)
                     /locus_tag="VNQ33_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97750.1"
                     /translation="MLAAAVASALLAQSSAPADTTGSNPGVVIVGVVIVTAVIATVVI
                     RRSKR"
     gene            complement(722..1486)
                     /locus_tag="VNQ33_06290"
     CDS             complement(722..1486)
                     /locus_tag="VNQ33_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWJ97751.1"
                     /translation="MATPTDPELLEAAVTIIRKAGDLTLQHFRRDDLAIERKGDGTPV
                     TVADRGAERLVREELERLFPDDGILGEEEAEKIGTSGRRWILDPIDGTKAFTHGVPLY
                     TNLLALEDEDGIAVGVINVPALGETVAAGRGLGCFANGAPVSVNDHASLEGACISTSG
                     FNIWSDEAMLRVKKTGMQLRTWGDGYGYVLVATGRIEAMVDPVAELYDLAPMPIILAE
                     AGGRFTSYAGEDGPGHGSGVATNGRVHDDVLDLLQD"
     gene            complement(1592..>2554)
                     /locus_tag="VNQ33_06295"
     CDS             complement(1592..>2554)
                     /locus_tag="VNQ33_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ97752.1"
                     /translation="GDDGWYYCYLHINNDDPGTDNGANQFKYAFAPGMATGKRVLKGD
                     HIAYLGDSGNAENVGSHCHFEIRMPNAKWYNAAAVNAKYSLDAAEPAKLRAKVADTAF
                     APLPNAAAFVKQQVADFLGIVPSATFVQRSVEDLEGGAVTPDEFTASLLTHPEATALT
                     APTTRLYLGFFLRIPDYSGLDYWIRKVRAGTTLDAAASQFAGGSEFIRRYGNLDNEAY
                     ITQMYQNLFSRDPDPSGLQYWKTRADAGAKRGWLMRQWCESSEYQRKTAAQVQVIQIH
                     AAMLHQAPSAAEYKRWSDWLGATPKGVTDLITYLRTGPTYQNRF"
BASE COUNT          331 a          812 c         1012 g          399 t
ORIGIN      
        1 gcagcagcac cagctcgccg gtgaccacgg tgtccttgcc gtcgacctcg agcggaaccg
       61 accaggtgcc gtcgtcgccg ccgagatcga cgcgcccgtc ggcggcgacg acgcgcggcg
      121 ccttcggcga catccagtgg atgcggtggt cgtgccaggc gtaggtgccg ttggtggcca
      181 cggtctccca gcggggagcc gcgccggggt cggggacggt ggacgaggcg ccgtagcgcg
      241 cctggttggc ggtggtggcc accgccaggg tgttgcggcg gaccgtgccg tcggcgtcga
      301 agcggaggta cgggaccggc tcggcttcgc cggacgtggg gtagtcggcg atgacggcgg
      361 tgtggccctt cggcagggag agctcgagca gggagtcgcc gccgatgacg cggaccgtgg
      421 cgccggaggg aagggcgggc gagacgtcgg tgaccgtcga ccggtagtcg gtgggccggg
      481 gtgcgtcggc cgaggccggc gaagccgaga cgagcagcag gaccagcgcc ccgagcgccg
      541 cgaggccagc tcgtgggacc ggccgtcgcg tcatcgcttc gatcggcgga tgacgacggt
      601 ggcgatgacc gcggtcacga tcacgacgcc cacgatcacg accccggggt tcgagcccgt
      661 ggtgtcggcc ggagccgacg actgggcgag gagggcggac gcgaccgcag cagccagcac
      721 gtcagtcctg cagcagatcc aagacgtcgt cgtgcacccg gccgttggtg gcgacgccgc
      781 tgccgtgccc agggccgtcc tcgccggcgt agctcgtgaa gcggccgccg gcctcggcga
      841 ggatgatcgg catgggagcg aggtcgtaga gctcggcgac gggatcgacc atggcctcga
      901 tgcggccggt ggccacgagc acgtacccgt agccgtcgcc ccaggtgcgc agctgcatgc
      961 cggtcttttt gacccggagc atcgcctcgt cgctccagat gttgaagccg ctggtgctga
     1021 tgcacgcgcc ctcgaggctc gcgtggtcgt tcaccgagac cggcgccccg ttggcgaagc
     1081 agccgagacc gcggccggcg gcgacggtct cgccgagtgc gggcacgttg atgacgccga
     1141 ccgcgatgcc gtcctcgtcc tcgagcgcca gcaggttcgt gtagagcggc acgccgtggg
     1201 tgaaggcctt ggtcccgtcg atggggtcga ggatccagcg ccggcccgac gtgccgatct
     1261 tctcggcctc ctcctcgccg aggatgccgt cgtcggggaa gagccgctcg agctcctcgc
     1321 ggaccaggcg ctcggcgccg cggtcggcca cggtgacggg ggtgccgtcg cccttgcgtt
     1381 cgatggcgag gtcgtcgcgg cggaagtgct ggagggtgag gtcccccgcc ttgcggatga
     1441 tggtgacggc tgcctcgagc agctccggat cggtcggcgt ggccatgcgc tcatcctccc
     1501 acgcggcccg gcgcgccgag ccagccggcc cgaggcggcc cgggtccgcg acgcccgccc
     1561 tcaggcggcg gtggccgcgc cggtccgggt cctagaaccg gttctggtag gtggggccgg
     1621 tgcgcaggta ggtgatgagg tcggtgacgc ccttgggcgt ggcgcccagc cagtcgctcc
     1681 agcgcttgta ctccgcggcg gagggggctt ggtggagcat cgccgcgtgg atctggatca
     1741 cctggacctg cgccgcggtc ttgcgctggt actcgctgga ctcacaccac tggcgcatga
     1801 gccagccccg cttggcgccg gcatcggctc gggtcttcca gtactggagg ccggacgggt
     1861 cggggtcccg cgagaacagg ttctggtaca tctgcgtgat gtacgcctcg ttgtcgaggt
     1921 tgccgtagcg gcggatgaac tccgaacctc ccgcgaactg gctggcggcg gcatcgagcg
     1981 tggtgccggc gcggaccttg cggatccagt agtcgaggcc ggagtagtcg gggatgcgga
     2041 ggaagaaccc gaggtagagc cgggtcgtgg gtgcggtgag cgcggtcgct tcggggtggg
     2101 tcagcaggct ggcggtgaac tcgtcggggg tgacggcgcc gccctcgagg tcctcgaccg
     2161 accgctggac gaaggtggcc gagggcacga tgccgaggaa gtcggcgacc tgctgcttca
     2221 cgaaggcggc ggcgttcggc aggggagcga acgcggtgtc ggcgaccttg gctcggagct
     2281 tggccggctc ggcggcgtcg agcgagtact tcgcgttgac ggccgccgcg ttgtaccact
     2341 tggcgttggg catccggatc tcgaagtggc agtggctgcc gacgttctcc gcgttgccgc
     2401 tgtcgccgag gtaggcgatg tggtcgccct tcaagacccg cttgccggtg gccatgcccg
     2461 gggcgaacgc gtacttgaac tggttggcgc cgttgtcggt gccgggatcg tcgttgttga
     2521 tgtggaggta gcagtagtac cagccgtcgt cgcc
//
LOCUS       DATMFS010000347         7758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_314889, whole genome shotgun sequence.
ACCESSION   DATMFS010000347 DATMFS010000000
VERSION     DATMFS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314889"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VNQ33_06300"
     CDS             complement(<1..669)
                     /locus_tag="VNQ33_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWJ97753.1"
                     /translation="MAEVSVSGPVSGTDKTLTLSTGVYAPQSQGAVVAKLGNTQVLAT
                     ANGAKEVREGTDFFPLTIDVEEKAYAAGKIPGSFFRKEGRPSDNAVLVCRLIDRPLRP
                     SFPKGFRNETQVVITVQGADQVNPYDVVAINAASASLMLTGLPFEGPIGGVRLAYSTD
                     GQWIPYPTFEEQDDATFQIVVAGREVDGEIAIMMVEAGGTEKAWSYYTDGAPKVTESV
                     IAGGL"
     gene            complement(871..1134)
                     /gene="rpsO"
                     /locus_tag="VNQ33_06305"
     CDS             complement(871..1134)
                     /gene="rpsO"
                     /locus_tag="VNQ33_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWJ97754.1"
                     /translation="MIDRQKPEVISEHRLHESDTGSPEVQIALLTKRINHLNEHLKVH
                     KKDHHSRRGLLMLVGRRRNLLDYVKNNDVERYRTIIATHGLRR"
     gene            complement(1480..2628)
                     /locus_tag="VNQ33_06310"
     CDS             complement(1480..2628)
                     /locus_tag="VNQ33_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ97755.1"
                     /translation="MGRSSSHGAASGGADDGTGIDVLATSEDETVLGPMPSDEVVSST
                     LPRLARHTIALADGHKVRVSVCGRGVPIVLIHGYSAEGMLYAQTLSRIVTSGFKVVAI
                     DVANHGGTQGLPTGGANFEEYTKLLGRVVDELGINKAVYMGHSMGGRLVTELAAREPD
                     RALAVILLDAIVGEPWDRIVAVSRFNPFLLAGVGAVLMADSLSTVPFVRDPRQAVKFS
                     RLAMPMVAGHVRNPLHLLGPGVSILRSRSSKWMLEKLGREGVPVIAIHGDRDIVVPLA
                     TARSAAKLAKGGVVVVEGGRHSWLLKDPETLPAILRELFDAPASVEARNRDFAELGLA
                     PDATLDEIEAAFYEPDAAVLALTPEIDLGSIKPGPRRPRFRWHVDAPA"
     gene            complement(2714..3670)
                     /locus_tag="VNQ33_06315"
     CDS             complement(2714..3670)
                     /locus_tag="VNQ33_06315"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWJ97756.1"
                     /translation="MQVIEGVGPCATPQPPSVVTIGAYDGVHLGHRAVIDQVKARAAD
                     QGLQTVVVTFDRHPAMVVRPESAPKLLCDLDQKLELLAEAGVDATYVVRFDEERAKET
                     AEEFVHEVLAGCLTARGVVVGEDFHFGHKRGGNVALLREMGESLGFTVTGLELVGPTG
                     APAGEGTVVSSTAVRSLLAEGDVGGAAAMLGRSHEVRGTVEHGDKRGRELGFPTANLR
                     IPAEIQLPADGIYAGWFERADGSVHQTAMSLGHRPTFYERPEGAPLLECNLLDFSGDL
                     YGEAVRVRFVERLRGEIKFDGIDPLVAQMAADVDQTRSLLRP"
     gene            4056..5126
                     /gene="rsgA"
                     /locus_tag="VNQ33_06320"
     CDS             4056..5126
                     /gene="rsgA"
                     /locus_tag="VNQ33_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWJ97757.1"
                     /translation="MTELLARYGWDDRWASRFHDYLRTPAAAEIDEPEPGRVIRHDGA
                     GLMVAVEAGAVRSMFGPPVQPPPVVGDWVVLDRHRTPVATLPRESLLRRRAAGTGEKE
                     QAMAANVDLVLVVCGLDRPVRTGRIQRTITIALDAGAESLVVLTKADKVGPEVAAEAE
                     MIAAEVDPGLEVVTLSAKGGWGVDDLLAHVGERTVALIGESGAGKSTLVNALMAGEVA
                     AEGDVRDGDSKGRHTTTHRELHLLDGGGVLVDTPGIREVGIWTDTDAVAESFPDIDEL
                     AEHCRFRDCAHESEPGCAVRAAVADGRLAQERLDAWRALDAEAAAAEMRADVVERHRR
                     NRQFGRIAREAQKLKDEQRNLP"
     gene            5295..6974
                     /locus_tag="VNQ33_06325"
     CDS             5295..6974
                     /locus_tag="VNQ33_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWJ97758.1"
                     /translation="MTLSYEDASAQITAPGERYETHEIEVGGITYTAFKGAPSSVKEL
                     ADLTRLYGDTEYLVYEDERYTYADVYARADSIAAALVDGYGVQKGDRVAIAMRNYPEW
                     IITYLGALSIGAVVVSMNAWWTAEEMAYGLEDSGAKVLVADLERVERSRGAAAALGFR
                     TIGVRLDEAEVPPGIERWEDVVVPGATRPEVEVGPDDDATILYTSGTTGRPKGAVSTH
                     RAIVQALMGFGCKTSIDSLRRPEEAAGRTGAPVFILIVPLFHVTGNVPVFLGSLASGL
                     KLVIMHKWDPGQALRLIEREKVTNFIGVPTQSWDLLEHPDFEKYDTSSLTSVGGGGAP
                     APPQLVGRIGSSFKTAKPNIGYGMTETNAYGPGNSGADYETHPTSTGRSTPILTVEIR
                     DADGQPVPAGQPGEIWMKGPNLIRGYWNKPEATAETIVDGWLRTGDLGRMDEEGFLSI
                     EDRAKDMILRGGENVYSAEVEAAVYEHPAVYEAAVFGLPHERLGEEVAVAIVPREGET
                     IDPDELRRFLADQIAPFKIPSKVFLFDEALPRNPAGKILKRQLRDELTEGA"
     gene            complement(7205..>7758)
                     /locus_tag="VNQ33_06330"
     CDS             complement(7205..>7758)
                     /locus_tag="VNQ33_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97759.1"
                     /translation="FGFFAADAYVPLAVVDGRGGSSWIAGAALTVSALTWTAGSWIQA
                     RSIDQVGPQRLDRIGFGALAVGIALMLGVALGLPVGLAVVAWAVSGFGIGIAYAPQAV
                     TVLASAKPGEEGKASAAIQLSDAVGIALGTGVAGGVISIGDGRGWPVDHSVTWVFVLA
                     LGGALGGVLASGRIPERVPAQAT"
BASE COUNT         1175 a         2781 c         2731 g         1071 t
ORIGIN      
        1 gaggccgccg gcgatgaccg actcggtcac cttgggggcg ccgtcggtgt agtagctcca
       61 ggccttctcg gtgccaccgg cctcgaccat catgatggcg atctcgccgt cgacctcacg
      121 gccggcgacg acgatctgga aggtggcgtc gtcttgctct tcgaacgtgg ggtacgggat
      181 ccactggccg tcggtgctgt acgccaggcg cacaccgccg atggggccct cgaagggaag
      241 gccggtgagc atcagcgacg ccgaggcggc gttgatggcg accacgtcgt aggggttcac
      301 ctggtcggcg ccctggacgg tgatgacgac ctgggtctcg ttgcggaagc ccttcgggaa
      361 cgacgggcgc agcggacggt cgatgaggcg gcacacgagc accgcgttgt ccgagggacg
      421 gccctccttg cggaagaacg agccggggat cttgccggcg gcgtaggcct tctcctcgac
      481 gtcgatggtc agcgggaaga agtcggtgcc ctctcggacc tccttcgcgc cgttggccgt
      541 ggccaggacc tgggtgttgc ccagcttggc gacgaccgca ccctgcgact gcggggcgta
      601 gaccccggtc gagagggtca gggtcttgtc ggtcccggaa acgggaccgg aaacgcttac
      661 ttcagccatg gttggctctc tcctctggag ccgtgtgctc cggtgtgagg cagatcccct
      721 gcggccagtt cccagtcgac gacctcccga cccgcatggc cgagcgatgg gcgactgaca
      781 actgacttcg tggaacccgc cggttgtgtt ggttacttgc gaaaagccca cgagcggccc
      841 cggagggccg ctcgtggcag agcttcggac ctagcggcgc aggccgtggg tggcgatgat
      901 cgtgcggtac cgctcgacgt cgttgttctt gacgtagtcg agcaggttgc ggcgacgacc
      961 caccagcatc agcaggccac gccggctgtg gtggtccttc ttgtgcacct tgaggtgctc
     1021 gttgaggtgg ttgatgcgct tggtgagcag cgcgatctgc acctcggggg acccggtgtc
     1081 gctctcgtgg aggcggtgct ccgagatgac ctcgggcttc tggcgatcga tgatctcaga
     1141 caagtctctt ctgttcctct tgggtttcgg acccagcccc tcaggccgca ggttctctgc
     1201 ctgcccagga gcggacgtgc cccgatcggg cacagggaac gacactagtg cccgaacccc
     1261 cgttcgacct aacccccacg agaagagagc cgctccgcgc cggctggttc gagcgaagcg
     1321 agctcggggg ggtgaggggg cggagcttgc ggagccccgt caagccagcg aggtgcgacg
     1381 gagctggcgg agtcgtgccg cgctgcgaag gtgcggcgtc aggccttccg ccagttcgag
     1441 gaacggagtg agcgccagcg aacgagtgac gagaggccgt caggccggtg cgtcgacgtg
     1501 ccagcgaaag cgcggccgac gggggccggg cttgatggag cccaggtcga tctcaggcgt
     1561 cagggcgagg acggcagcgt cgggctcgta gaaggcggcc tcgatctcgt cgagggtcgc
     1621 gtcaggagcc aggcccagct cggcgaagtc ccggttgcgg gcctcgacgc tggccggggc
     1681 gtcgaagagc tcccggagga tggccggcag ggtctcgggg tccttcagca gccacgagtg
     1741 gcggccgccc tcgaccacga cgacaccgcc cttggccagc ttggcggcgc tgcgggccgt
     1801 ggccaggggc acgacgatgt cgcggtcgcc gtggatcgcg atcaccggca cgccctcgcg
     1861 gccgagcttc tcgagcatcc acttcgacga gcgggaccgg aggatcgaca cgcccggtcc
     1921 gaggaggtgg agcgggttgc gcacgtggcc ggccaccatc ggcatcgcca ggcggctgaa
     1981 cttcacggcc tggcgggggt ctcgcacgaa cgggacggtg ctcagcgagt cggccatgag
     2041 gacggccccc acgccggcga gcaggaacgg gttgaagcgg gacacggcca cgatgcggtc
     2101 ccagggctcg cccacgatgg cgtcgagcaa gatcacggcc agagcccggt ccggctcgcg
     2161 ggcggcgagc tccgtgacga ggcgaccgcc catcgagtgg cccatgtaga cggccttgtt
     2221 gatgccgagc tcgtcgacca cgcggccgag gagcttcgtg tactcctcga agttggctcc
     2281 gccggtgggc agcccctggg tcccgccgtg gttggcgacg tcgatggcga cgaccttgaa
     2341 gcccgacgtg acgatgcggc tcagcgtctg ggcgtagagc atcccctcgg ccgagtagcc
     2401 gtggatcagc acgatcggca cgccgcggcc gcagaccgac acgcgcacct tgtggccgtc
     2461 agccagggcg atggtgtggc gggcgaggcg gggcagcgtc gacgagacga cctcgtcgga
     2521 gggcatgggg cccaggacgg tctcatcctc ggacgtggcg aggacgtcga tgccggtgcc
     2581 gtcgtcggcg ccacccgatg cggcgccgtg tgagctggac ctacccatgt gggctcccct
     2641 tccccccttg cacccgcgct gacgtggaca tcaagtgtgg cgcaccgatc cgatcagcag
     2701 cgcggaccct ccctcaagga cgcagcagcg aacgggtctg gtcgacgtcg gcggccatct
     2761 gggcgacgag cgggtcgatc ccgtcgaact tgatctcgcc ccgcagccgt tcgacgaacc
     2821 ggacgcgcac cgcctcgccg tagaggtcac ccgagaagtc gagcaggttg cactcgagca
     2881 gcggggcccc ctccggacgc tcgtagaagg tgggtcggtg cccgagcgac atcgcggtct
     2941 ggtggacgga gccatcggcc cgctcgaacc agccggcgta gatgccgtcg gccgggagct
     3001 ggatctcggc ggggatccgg aggttggcgg tcgggaaccc gagctcgcgg ccccgcttgt
     3061 cgccgtgctc cacggtgccg cggacctcgt gggaccggcc gagcatcgcc gccgcacccc
     3121 cgacatcgcc ctcggcgagc agcgagcgca cggcggtcga cgacacgacc gtgccctcgc
     3181 ccgccggggc accggtcggg ccgaccagct ccaggccggt gacggtgaag cccaggctct
     3241 cgcccatctc gcgcagcagg gcgacgttgc ccccgcgctt gtgcccgaag tggaagtcct
     3301 cgcccaccac gaccccacgg gcggtgaggc agccggcgag cacctcgtgg acgaactcct
     3361 ccgccgtctc cttcgcccgc tcctcgtcga agcggacgac gtaggtggcg tcgacaccgg
     3421 cctcagccag cagctcgagc ttctggtcca ggtcgcacag cagcttgggc gccgactcgg
     3481 gccgcacgac catcgccgga tggcggtcga acgtgaccac gacggtctgc agtccctggt
     3541 ccgccgcccg cgccttcacc tggtcgatga cggcgcggtg acccaggtgg acgccgtcgt
     3601 aggcgccgat ggtgaccacg ctggggggct ggggcgtggc gcacggcccg acgccctcga
     3661 tgacctgcac gccgaggacg ctagggacgc accgccacga gggccaactc cgatctggtg
     3721 ggcggcgcca cccggtgcct ggcccgctcg acctcggccg ggacctcgag cggtaccggg
     3781 ccgagaccgc aggcggctgc tgacaccaga ggcggtccgg tggtgggcca gcaggcggct
     3841 ggcgcgctcc gccctgctgg cgaagtcccg gcgacccggg accgagcctg cggtagcgtt
     3901 cggcccgtcc gcagagatcg agcgcaccag gaggaagacg ccatggctgc cacccgctct
     3961 tgcctcctgc cgcgcgcctg accgaacagg gagcgcggct tgtccccgtg ctctgacggc
     4021 accgcggccg cggtgccctg ttccaaggag ctccgttgac cgaactgctc gcccgctacg
     4081 gatgggacga ccgctgggcg tcccgcttcc acgactacct caggactcca gccgccgccg
     4141 agatcgacga acccgagccc ggacgggtga tccgccacga cggtgccggc ctgatggtcg
     4201 ccgtcgaggc cggcgccgtc cgctccatgt tcggcccgcc cgtccagccg ccacccgtgg
     4261 tcggcgactg ggtggtgctg gaccgccacc gcacccccgt cgccaccctc ccgcgtgagt
     4321 cgctcctgcg ccgccgcgcc gccggcaccg gcgagaagga gcaggccatg gccgccaacg
     4381 tcgacctcgt gctggtcgtc tgcggcctcg accggcccgt ccgcaccggc cgcatccagc
     4441 gcaccatcac catcgccctc gatgccggcg ccgaatcgct cgtcgtgctc accaaggccg
     4501 acaaggtcgg ccccgaggtg gccgccgagg ccgagatgat cgccgccgag gtggaccccg
     4561 gcctcgaggt cgtcaccctc tcggccaaag gcggctgggg cgtcgacgac ctgctcgcgc
     4621 acgtcggcga gcgcaccgtt gccctcatcg gcgaatcggg cgcgggcaag tcgaccctcg
     4681 tgaacgcgct catggccggc gaggtggccg ccgagggcga cgtgcgcgac ggcgactcga
     4741 agggccgcca caccaccacc caccgcgagc tgcacctgct cgacggcggc ggagtcctcg
     4801 tcgacacccc gggcatccgc gaggtcggca tctggaccga caccgacgcc gtcgccgagt
     4861 ccttccccga catcgacgag ctggccgagc actgccggtt ccgggactgc gcccacgagt
     4921 ccgagcccgg ctgcgcggtc cgagccgccg tcgccgacgg caggttggcc caggagcgcc
     4981 tcgacgcctg gcgggccctc gacgccgagg ccgccgcggc cgagatgcgc gccgacgtgg
     5041 tggagcgcca ccgccgcaac cgccagttcg gccgcatcgc acgcgaggcc cagaagctca
     5101 aagacgagca gcgcaacctc ccctgaccag atttcgggtc acctgagctc ctctccggtg
     5161 cccaggtgac ccgaaaggct cgggtcggcc ccgctcccac gatttcgggt cacgcgtgct
     5221 cgccagaggt gcacagatga cccgaaaccg gtcgcgcttg accgacgggt cagggtgggg
     5281 ttggctccct tggcgtgacc ctcagctacg aagacgcctc tgcccagatc accgcacccg
     5341 gggaacggta cgagacccac gagatcgagg tggggggcat cacgtacacg gcgttcaagg
     5401 gggcgccgtc gtcggtgaag gagctggccg acctgacccg cctctacggc gacaccgagt
     5461 acctcgtcta cgaggacgag cggtacacgt acgccgacgt ctacgcccgc gccgacagca
     5521 tcgccgccgc cctcgtggac ggctacgggg tgcagaaggg cgaccgggtg gccatcgcca
     5581 tgcgcaacta cccggagtgg atcatcacct acctcggggc cctgtcgatc ggcgccgtcg
     5641 tggtgtcgat gaacgcctgg tggaccgccg aggagatggc ctacggcctc gaggactcgg
     5701 gcgccaaggt cctcgtcgcc gacctcgaac gggtcgagcg cagccgcggt gcggccgcgg
     5761 cgctcgggtt ccgcaccatc ggcgtccgcc tcgacgaggc ggaggtgccg cccggcatcg
     5821 agcgctggga ggacgtcgtc gtgcccgggg ccacccggcc cgaggtcgag gtcggccccg
     5881 acgacgacgc caccatcctc tacacgtccg gcaccaccgg ccggccgaag ggcgcggtgt
     5941 ccacgcaccg ggccatcgtc caggccctga tgggcttcgg ctgcaagacg tcgatcgact
     6001 ccctgcgccg ccccgaggag gccgcgggcc gcaccggtgc cccggtgttc atcctcatcg
     6061 tccccctctt ccacgtgacc ggcaacgtgc cggtcttcct cggttcgctc gcctccgggc
     6121 tgaagctcgt gatcatgcac aagtgggatc ccggccaggc gctccggctg atcgagcggg
     6181 agaaggtcac caacttcatc ggcgtgccca cccagagctg ggacctgctc gagcacccgg
     6241 acttcgagaa gtacgacacg tcgtcgctca cctccgtcgg cggtggcggc gcccccgctc
     6301 ccccgcagct cgtgggccgg atcggcagct cgttcaagac cgccaagccc aacatcggct
     6361 acggcatgac cgagaccaac gcctacggcc cgggcaacag cggcgccgac tacgagaccc
     6421 atcccaccag cacggggcgc tccacaccga tcctcacggt cgagatccgc gacgcggacg
     6481 gccaacccgt gcccgccggt cagcccggcg agatctggat gaagggcccg aacctcatcc
     6541 ggggctactg gaacaagccc gaggccaccg ccgagaccat cgtcgacggc tggctgcgca
     6601 ccggcgacct cggccgcatg gacgaggagg gcttcctctc catcgaggac cgggcgaagg
     6661 acatgatcct gcgcggcggg gagaacgtgt actccgccga ggtcgaggcc gccgtctacg
     6721 agcacccggc ggtctacgag gcggcggtgt tcggcctgcc ccacgagcgc ctcggcgagg
     6781 aggtcgccgt ggcgatcgtc ccccgcgagg gcgagaccat cgaccccgac gagctgcgcc
     6841 gcttcctggc cgaccagatc gcccccttca agatcccgtc gaaggtcttc ctcttcgacg
     6901 aggccctccc ccgcaaccct gccggcaaga tcctcaagcg ccagctccgc gacgagctca
     6961 ccgagggcgc ctgatcccgc tggcccctgg cgggaccgca cccggtcctc gtcggtgctc
     7021 ccgtgcaccc tcgccatcgc ctcttccccg gcttgccatc aagttggagg atcccggtcg
     7081 ctcaccgacc acgaccctcc accttccgcc gccagcctcc aacttggagc atctcggtct
     7141 caaaccgcca aaaccctcca agttggaggc tgagagccgt gggcggtcga gccgtgcgtc
     7201 cgggtcaggt ggcttgggcg gggacgcgtt cggggatgcg gccggaggcg aggacaccgc
     7261 cgagggcgcc gccgagggcg aggacgaaga cccaggtgac gctgtggtcg accggccagc
     7321 cgcggccgtc gccgatcgag atgacgccac cggcaacgcc ggtgccgaga gcgatgccga
     7381 ccgcgtcgct caactggatc gccgcgctgg ccttgccctc ctcccccggc ttcgccgagg
     7441 ccagcacggt gacggcctgg ggcgcgtagg cgatgccgat gccgaacccg gagacggccc
     7501 acgccacgac cgcgaggccg acagggaggc cgagggcgac gccgagcatc agggcgatgc
     7561 cgacggcgag ggcgccgaag ccgatgcgat cgagccgctg aggcccgacc tggtcgatgg
     7621 agcgcgcctg gatccacgaa ccagcggtcc aggtgagcgc gctcacggtc agcgccgcac
     7681 cggcgatcca gctcgatccg ccgcggccgt cgaccaccgc gagggggacg taggcgtcgg
     7741 cggcgaagaa cccgaagg
//
LOCUS       DATMFS010000348         5363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_81866, whole genome shotgun sequence.
ACCESSION   DATMFS010000348 DATMFS010000000
VERSION     DATMFS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81866"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VNQ33_06335"
     CDS             complement(<1..205)
                     /locus_tag="VNQ33_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97760.1"
                     /translation="MELISIPLAGGPAQVMVQDLPAVAGPHGVLGVDVAARTIEGRHW
                     TVVQLLLDDDHPLFDRSLLDAPVV"
     gene            241..603
                     /locus_tag="VNQ33_06340"
     CDS             241..603
                     /locus_tag="VNQ33_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWJ97761.1"
                     /translation="MVTEDDVRRIALELPEVIEKPSYGTPGFRVKDTLFLRIRSEAEG
                     GLVVFTEDLGEKEALLASDPATFFTTPHYDGHASVIVHLEEVDVDELTELITDSWLVK
                     APPKVRKDHPEVGRVDGA"
     gene            706..2445
                     /locus_tag="VNQ33_06345"
     CDS             706..2445
                     /locus_tag="VNQ33_06345"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWJ97762.1"
                     /translation="MGRLRIASCQLNTRVGALDHNVTAILDALHAAEEAGCDLAVFPE
                     LAVTGYPPEDLLLKPGFIADNRKALEKVAAETRSCVAVVGFVDAGRDLWNAAAVCAHG
                     EVVGVYHKRELPNYAVFDEARYFARGAEPAQLWSVGGVSVGVSICEDAWNPAGPILDQ
                     ADSGAELIVNLNASPYAEGKLARRERMMATRAADASCALVYVNQVGGQDELVFDGGSM
                     VFDAEGTLVARSPQFVDDVMIVDLDVEPVYRKRLLDPRGRPTDLLLPIEMTRGEEVEA
                     SEARRNSSEAPLLSATVAAPLDPDEEVYRAIVLGTRDYLVKNGFTDVVIGLSGGIDST
                     LVAAVAVDAIGADRVHGVSMPSRYSSDHSMSDAALLAENLGITDFRTIAIEPGHAALL
                     EMLAPSFEGRGADLTEENLQSRLRGMTLMALSNKFGWIVLSTGNKSEVAVGYATLYGD
                     TVGGYSLLKDVYKTRVYDLCRWRNTQGLHPVIPEGVIAKPPSAELRPDQRDDQSLPPY
                     DVLDPILEGYIEHDRTVAELIARGHDEDTVRRVTRLVDNAEYKRRQSPIGVKVTAKAF
                     GKDRRLPITNGFR"
     gene            2465..3439
                     /locus_tag="VNQ33_06350"
     CDS             2465..3439
                     /locus_tag="VNQ33_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWJ97763.1"
                     /translation="MTVAGSAPLPSRARGRWVLALAGFLFGSTFLVVQGAVDRAAVLP
                     FLAVRFLIGAAVLWPLARRNPATPREVRHGILAGLTLLAGFLLQTTGLKTTTSATSAF
                     ITYLLVVIVPLIGVVRTRKAPPLNVLVGVLLATAGLSLLSGGTAGIGRGEVLTVGAAF
                     FFALHIVVLGEVSERHDPIRLTMWQALTVGVCCFFPGFFAPGGYSFDAGVWFAAAFCG
                     VGATAAAFWCMTWAQRVVPESQAAIILLLEPVSAAVLGALAGEALGLAGAVGAVLILA
                     SVLVAELAGGSHPSAVGAELVLLHEDAAGSGACDGVDEATASGQPPPS"
     gene            3436..3963
                     /locus_tag="VNQ33_06355"
     CDS             3436..3963
                     /locus_tag="VNQ33_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97764.1"
                     /translation="MTDPAADLVVITPPGLPILDAAGWIGHACWVELRLHAALTHWLS
                     IEPDPEAVAVYWIERADAAERAEAWHRRLPELREHPRSDFVVASSDRVAHVLNQLTLL
                     DQPDRTEQRRGALSAVLRGLRLGYHAHQDRAVGPADGPTATTLNAALRSTFGTGGADP
                     DPAWTQAVADAGGLP"
     gene            4251..4643
                     /locus_tag="VNQ33_06360"
     CDS             4251..4643
                     /locus_tag="VNQ33_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97765.1"
                     /translation="MIIRQRTSWDLTLAHDLFYENNDVFAHKMGGFKCHANRSGKQAT
                     RCVMRLLHNQTDIPGLVKGVWGRATAGDEPIRFDDVNGAFERVRATEDNKAQTAECLT
                     LSVYPHTVNWTERSRSDANGRLGEHVWD"
     gene            4643..5032
                     /locus_tag="VNQ33_06365"
     CDS             4643..5032
                     /locus_tag="VNQ33_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97766.1"
                     /translation="MGKRTLVVGLVVTVVVSLLILADRCGRNDPVEAPVFEPATSFCA
                     EFRASTFVQQIEGVSNTRTDLRPRVARVEAGFLRQIGELDDVPGDARPLLRELAGEVA
                     AGARTGTFGDAAEVAAALDGVAAERCR"
     gene            complement(5041..>5363)
                     /locus_tag="VNQ33_06370"
     CDS             complement(5041..>5363)
                     /locus_tag="VNQ33_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97767.1"
                     /translation="LRTEEVTFDVKLRAVVSTRLREVPGPPGWAGFDTPLDSSDSGWS
                     ITAGDEPADFFGDGEAPDTGTEAMALGELVQRFPALVEVFQAGEGEWVYRPDHRRYVR
                     LKNT"
BASE COUNT          734 a         1936 c         1856 g          837 t
ORIGIN      
        1 cgacgacggg ggcgtcgagc aggctccggt cgaacagcgg atggtcgtcg tcgagcagga
       61 gctgcacgac cgtccagtgc cggccctcga tcgtgcgggc ggcgacgtcg accccgagca
      121 caccgtgggg gccggcgacc gcggggaggt cctggaccat cacctgtgcc ggtccgccgg
      181 ccagcgggat cgagatcagc tccacgcctg gagcctaggg agcggcctac ggtggcgctc
      241 gtggtcaccg aggacgacgt gcggcgcatc gctctggagc ttcccgaggt gatcgagaag
      301 ccctcgtacg gcacgcccgg gttccgggtg aaggacacgc tgttcctgcg gatccgctcc
      361 gaggccgagg gcggcctcgt ggtcttcacc gaggacctcg gcgagaagga ggcgctcctc
      421 gcctccgacc cggccacgtt cttcacgacc ccgcactacg acggccacgc ctcggtgatc
      481 gtccacctcg aggaggtcga cgtcgacgag ctcaccgagc tgatcaccga ctcctggttg
      541 gtgaaggcgc cgccgaaggt ccgcaaggac caccccgagg tcggtcgcgt cgacggagcc
      601 tgaggcacgc ggcggggtcg cgccggaccg tccggcacgc cgccggagcc gaccgatcgc
      661 ggacggccgg ggccgatcgg ggtggcccat gacatgcttt cgggcatggg tcgcctccgg
      721 atcgccagct gccagctcaa cacccgcgtc ggcgcgctcg accacaacgt cactgccatc
      781 ctcgatgcgc tgcacgcggc ggaggaggcc gggtgcgacc tggccgtctt cccggagctg
      841 gccgtcaccg ggtacccgcc ggaggacctg ctcctgaagc cggggttcat cgccgacaac
      901 cgcaaggccc tggagaaggt ggcggccgag acccgcagct gcgtcgccgt cgtcgggttc
      961 gtcgatgccg gccgcgacct gtggaacgcc gccgcggtct gcgcccacgg cgaggtcgtc
     1021 ggcgtgtacc acaagcgcga gctgcccaac tacgccgtgt tcgacgaggc ccggtacttc
     1081 gcccggggcg ccgagccggc ccagctgtgg tcggtgggcg gcgtcagcgt cggggtcagc
     1141 atctgcgagg acgcctggaa ccccgccggc ccgatcctgg accaggccga cagcggcgcc
     1201 gagctgatcg tcaacctgaa cgcctccccc tacgccgagg gcaagctggc ccgccgggag
     1261 cggatgatgg ccacccgggc cgccgacgcg tcgtgcgcgc tcgtctacgt gaaccaggtg
     1321 ggtggacaag acgagctcgt cttcgacggc ggctcgatgg tcttcgacgc cgagggcacg
     1381 ctcgtcgccc ggtccccgca gttcgtggac gacgtcatga tcgtcgacct cgatgtcgag
     1441 ccggtgtacc ggaagcggct gctcgatccc cgcggtcgtc cgaccgatct gctgctgccg
     1501 atcgagatga cccgaggcga ggaggtcgag gcctccgagg cgcgccgcaa ctcgtcggag
     1561 gcaccgctgc tgtcggccac cgtcgcggcc ccgctcgacc ccgacgagga ggtgtaccgg
     1621 gcgatcgtgc tcggcacgcg cgactacctc gtgaagaacg gcttcaccga cgtcgtgatc
     1681 ggcctgtccg gcggcatcga ctcgacgctc gtggccgcgg tggccgtcga cgccatcggc
     1741 gccgaccgcg tgcacggcgt gtcgatgccc tcgcgctatt cgagcgacca ctccatgagc
     1801 gacgccgccc tcttggccga gaacctcggc atcaccgact tccgcaccat cgccatcgag
     1861 cccggccacg ccgcgctcct ggagatgctg gcgccgtcgt tcgagggccg cggcgccgac
     1921 ctcaccgagg agaacctgca gagccgcctc cggggcatga ccctcatggc cctctccaac
     1981 aagttcgggt ggatcgtgct gtcgacgggc aacaagtccg aggtggccgt cggctacgcc
     2041 accctctacg gcgacaccgt cggcggctac tcgctcctca aagacgtcta caagacccgc
     2101 gtctacgacc tctgccgctg gcggaacacg cagggcctgc atccggtgat ccccgagggc
     2161 gtcatcgcca agccgccgtc ggccgagctc cgaccggacc agcgcgacga ccagagcctc
     2221 ccgccctacg acgtgctcga cccgatcctc gagggctaca tcgagcacga ccggaccgtg
     2281 gccgagctca tcgcccgtgg ccacgacgag gacaccgtgc gccgcgtcac gcgcctggtc
     2341 gacaacgccg agtacaagcg ccgccagagc cccatcggcg tcaaggtcac ggccaaggcg
     2401 ttcggcaagg accggcgcct gccgatcacc aacggcttcc ggtagtgccg aacgtggcgt
     2461 cggcgtgacc gtcgctggga gtgcgcccct gccgtcgcgc gctcgtgggc ggtgggtcct
     2521 ggcccttgcc gggttcttgt tcgggtcgac gttcctggtg gtgcaggggg ccgtcgaccg
     2581 ggccgcggtg ctgccgttcc tcgccgtccg cttcctcatc ggggccgcgg tgctgtggcc
     2641 gctcgcccga cggaacccgg cgacgccccg cgaggtgcgc cacggcatct tggccgggct
     2701 cacgctgctg gccggcttct tgctccagac caccggcttg aagaccacca cgtcagcgac
     2761 atcggccttc atcacctacc tgctcgtggt gatcgtcccg ctgatcggcg tcgtgcggac
     2821 tcgcaaggca ccgccgctca acgtgctcgt cggcgtgctg ctcgccaccg ccgggttgtc
     2881 cctcctctcg ggcggcaccg cgggcatcgg ccggggtgag gtgctgacgg tgggtgcggc
     2941 gttcttcttc gcgctccaca tcgtggtgct cggcgaggtg tccgagcgcc acgaccccat
     3001 ccgcctcacg atgtggcagg cgctcacggt cggcgtgtgc tgcttcttcc ccggcttctt
     3061 cgcccctggc ggctactcgt tcgacgccgg cgtctggttc gccgctgcct tctgcggcgt
     3121 cggggccacg gcggcggcgt tctggtgcat gacgtgggcg cagcgggtgg tgcccgagtc
     3181 gcaggcggcg atcatcctgc tcctcgagcc ggtgtccgcc gccgtcctcg gcgccctggc
     3241 cggtgaggcg ctgggcctgg ccggcgccgt cggggcggtg ctgatcctcg cctcggtcct
     3301 ggtcgccgag ctggcgggtg gcagccatcc gtcggcggtc ggcgccgagc tcgtcctcct
     3361 ccacgaggat gccgccggga gcggtgcgtg cgacggtgtt gacgaagcga cagccagcgg
     3421 ccaacctcca ccgtcgtgac cgatcccgcc gccgacctcg tcgtcatcac cccgccgggg
     3481 ctcccgatcc tcgacgcggc cggctggatc ggccacgcgt gctgggtgga gctgcggctc
     3541 cacgcggccc tcacccactg gctctcgatc gagcccgacc ccgaggcggt cgccgtctac
     3601 tggatcgagc gggccgatgc cgccgagcgc gccgaggcat ggcaccgccg cctgcccgaa
     3661 ctgcgcgagc acccccgatc cgacttcgtc gtcgcctcgt ccgatcgcgt cgcccacgtg
     3721 ctcaaccagc tgacgctgct cgaccagccc gaccgtaccg agcagcggcg aggcgccctg
     3781 tctgcggtcc tgcgcggcct gcgcctcggc taccacgccc accaggaccg ggcggtcggt
     3841 ccggccgacg gtcccaccgc caccaccctc aacgctgcgc tgcgctccac cttcggcacc
     3901 ggcggcgccg accccgaccc cgcctggacc caagcggtgg ccgacgccgg cggcctcccc
     3961 tgagcctggt cggtatttat acgtatcgtc tttgcgacga ctgccttgct ccggtacggt
     4021 gcgcctcgtc gatgtgacct tcggtcgtca ccctgcgtcg gctcgatcca acatcgagtc
     4081 acagggagtg cgccatgtcc agggacccat ccccgccgcg ccgcggccac tgatcctcgc
     4141 agcgccgttg cgcttggctg ctcgtcggca tcctggcatt tctgctctcc tcgtgttcgt
     4201 ggacggctgg agcgcagtac ttcgttcact gggggccggc ccgcgacgac gtgatcattc
     4261 ggcagcggac gagttgggac ctcacgctgg cacatgacct cttctacgag aacaacgatg
     4321 tgttcgccca caagatgggt ggcttcaagt gccacgccaa tcgatctggg aagcaggcga
     4381 cccgctgtgt catgcgcttg ctgcacaacc aaacggacat ccccggtctg gtcaaggggg
     4441 tgtgggggcg agccacggcg ggtgacgagc ccatccgctt cgacgacgtc aacggtgcgt
     4501 tcgaacgggt acgcgcgacc gaggacaaca aggcgcagac cgccgagtgc ctgaccctca
     4561 gtgtgtatcc ccacaccgtg aactggacgg aacgcagccg gtccgacgcg aacggccgac
     4621 tcggcgagca cgtgtgggac tgatggggaa gcgcacgctt gtcgtcgggc tcgtcgtgac
     4681 ggtcgtggtt tccttgctga tcctcgcgga tcgctgtggg cgaaacgacc cggtcgaagc
     4741 tccggtgttc gaaccggcga cctcgttctg tgcggagttt cgtgccagca cgttcgtgca
     4801 gcagatcgag ggcgtctcca acacgaggac cgacctccgc cccagggttg cgcgggtcga
     4861 ggcgggcttc ctccgtcaga tcggcgagtt ggacgatgtg cccggcgatg cgcgcccgct
     4921 cctgcgtgag ctggcagggg aggtggccgc gggtgcccgc acggggacgt tcggtgacgc
     4981 ggcggaggtg gcggcagcgc tggacggcgt ggctgccgag cgctgccgat gaggaatggg
     5041 tcaggtgttc ttgaggcgga cgtagcggcg gtggtcgggt cggtagaccc actcgccctc
     5101 gccggcctgg aacacctcga ccagcgcggg gaagcgctgg accagctcgc cgagggccat
     5161 ggcctcggtc ccggtgtcag gcgcctcgcc gtcgccgaag aagtcggctg gctcatcacc
     5221 cgccgtgatg ctccagccgc tgtccgagct gtcgagggga gtgtcgaacc ctgcccaccc
     5281 aggaggcccg ggcacctcgc gcagccgggt gctgaccacg gcccggagct tcacgtcgaa
     5341 ggtgacctct tcggtgcgta ggg
//
LOCUS       DATMFS010000349         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_243395, whole genome shotgun sequence.
ACCESSION   DATMFS010000349 DATMFS010000000
VERSION     DATMFS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243395"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNQ33_06375"
     CDS             <1..271
                     /locus_tag="VNQ33_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97768.1"
                     /translation="LSNEEYQQGVARALCLRDFTIPEGTSTYPISVVAAVNGCTLAEV
                     EDQSLGPRCNPDGSMPALPSGEYQLRIDDAQQLVPAIDPVTVTVT"
     gene            complement(300..797)
                     /locus_tag="VNQ33_06380"
     CDS             complement(300..797)
                     /locus_tag="VNQ33_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97769.1"
                     /translation="MKIEPAGIEVPDDKDPIFYVWGEMVPGIGIAASTFAQSVYEHTT
                     LGLREFEAARLRIAQINGCLFCQDWRTDMGGEQVEESFDTAVEHWRTTDAFDDRTRLA
                     AEYAERFAVDHHTIDAAFWERMNAHYSQQEIVELSMCLGSWISFGRLNHVLGLDAACT
                     LPSHR"
     gene            complement(794..1891)
                     /locus_tag="VNQ33_06385"
     CDS             complement(794..1891)
                     /locus_tag="VNQ33_06385"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97770.1"
                     /translation="MVDQGTIGTVVWGTGNMGRAAIRAVEAHRGLGLAAVLVANPDKV
                     GRDAGDLAGLGRTLGVAASTDVEGILAAGPAAVAYTASGDIRPDDAVADVCRALRAGA
                     VVVTPAIYPLYDPTSAPAEIIDPVRQAIAEGGGKLFVSGIDPGWGNDILPALLTSVSS
                     TIDQVRCQEIFDYSTYDQPDSVRYLVGMGQPMDYEPMMVAPTVPTMVWGGQVRLIARA
                     LGVELDEIRETLERRALAETVENVMGTFDAGTQGGLRFEVQGIVDGEPVIVIEHVTRI
                     HPSVAPDWPSPADGGAGAHTVVIEGRPRITLSLEATDEGGNRAAGGNATAAGRLVNAI
                     PWLTAADPGVYDALDVPLTPATGKLGRKATA"
     gene            2043..>2308
                     /locus_tag="VNQ33_06390"
     CDS             2043..>2308
                     /locus_tag="VNQ33_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97771.1"
                     /translation="MRAGEVEAVLKRIGRVFILGLVLQPRISGQAVLLPVFGALMVWA
                     LFGLDRIAESPEGHRGLRATAGAAVAVTLLGFGGWIGVEPGGLSL"
BASE COUNT          307 a          787 c          861 g          353 t
ORIGIN      
        1 gctgagcaac gaggagtacc agcaaggtgt ggcccgtgcc ctgtgccttc gggacttcac
       61 gatcccggag ggcacatcga cctacccgat ctccgtcgtc gcggcggtga acggctgcac
      121 cctggccgag gtcgaagacc agagcctcgg accccgctgc aaccccgacg gctcgatgcc
      181 cgccctccca tccggcgagt accagctccg gatcgacgac gcgcagcagc tcgtccccgc
      241 catcgacccg gtcacggtca ccgtcacctg atcgacctcg gcacggcggc cgcgcccggt
      301 cagcggtggc tggggagggt gcaggcggcg tcgaggccga ggacgtggtt gaggcggccg
      361 aagctgatcc acgagccgag gcacatcgac agctccacga tctcctgctg ggagtagtgg
      421 gcgttcatcc gctcccagaa ggcggcgtcg atggtgtggt ggtcgacggc gaagcgctcg
      481 gcgtactcgg cggccaggcg ggtgcggtcg tcgaaggcat cggtggtgcg ccagtgctcc
      541 accgcggtgt cgaaggactc ctcgacctgc tcgccgccca tgtccgtgcg ccagtcctgg
      601 cagaacagac agccgttgat ctgagcgatg cggaggcggg cggcctcgaa ctcgcggagt
      661 ccgagggtcg tgtgctcgta gacgctctgg gcgaacgtcg acgccgcgat cccgatgccg
      721 gggaccatct cgccccacac gtagaagatg gggtccttgt cgtcggggac ctcgatgcct
      781 gcgggctcga tcttcatgcg gtggccttcc ttccgagctt gccggtggcg ggggtgagcg
      841 ggacgtcgag cgcgtcgtag acgccggggt cggcggcggt cagccacggg atggcgttga
      901 ccagccggcc ggcggcggtg gcgttgccgc cggcggcgcg gttgccgccc tcgtcggtgg
      961 cctcgaggct gagcgtgatg cggggacggc cctcgatgac gacggtgtgg gccccggcgc
     1021 cgccgtcagc gggcgacggc cagtccggtg cgaccgacgg gtggatgcgc gtgacgtgct
     1081 cgatgacgat gacgggctca ccgtcgacga tgccctgcac ctcgaagcgc aggccgccct
     1141 gggtcccggc gtcgaacgtg cccatcacgt tctccaccgt ctccgccagc gctcgtcgtt
     1201 cgagcgtctc gcggatctcg tcgagctcga cgccgagcgc ccgggcgatg aggcgtacct
     1261 ggccgcccca gaccatcgtc ggcacggtgg gtgccaccat catcggctcg tagtccatgg
     1321 gctggcccat gccgacgagg tagcggaccg agtcgggctg gtcgtaggtg gagtagtcga
     1381 agatctcctg gcagcggacc tggtcgatgg tgctggacac gctggtgagc agcgccggga
     1441 ggatgtcgtt gccccagccc gggtcgatgc ccgagacgaa cagcttcccg ccgccctcgg
     1501 cgatggcctg tcgcacgggg tcgatgatct cggccggcgc gctggtcggg tcgtacagcg
     1561 ggtagatcgc cggcgtgacg acgacggccc cggcgcgcag ggcgcggcac acatcggcca
     1621 cggcgtcgtc gggacggatg tcgcccgagg cggtgtaggc gacggccgcc ggacccgcgg
     1681 cgaggatgcc ctcgacgtcg gtggaggcgg cgacgccgag cgtccggccg agtccggcca
     1741 ggtcgccggc atcgcgcccg accttgtccg ggttggcgac gagcaccgcc gccagcccca
     1801 agccgcgatg ggcctcgacg gcccggatgg ccgcccgccc catgttcccg gtgccccaca
     1861 cgacggtccc gatggtcccc tggtcaacca tcgcgccgac cctaccgacg cctgccgacg
     1921 attgctgacg acccgtcaga tcagccgatc ccttcccgag cctcgtgtgg ctctggtcca
     1981 tggacgaacc cgtcccccac gcagttcgga gggggcgggg gtggccagta gcgtcgcccg
     2041 ccatgcgtgc cggggaggtc gaagcggtcc tgaagcggat cgggcgggtc ttcatcttgg
     2101 gcctggtcct ccagccgcgc atcagcgggc aggccgtgct gctgccggtg ttcggggcgc
     2161 tgatggtctg ggcgctgttc gggctcgacc ggatcgccga gtcgcccgag ggccaccgag
     2221 gcctgcgggc caccgcggga gcggctgtgg ccgtgaccct gctcgggttc ggcgggtgga
     2281 tcggggtcga accgggtggt ttgagcct
//
LOCUS       DATMFS010000350         2602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_35887, whole genome shotgun sequence.
ACCESSION   DATMFS010000350 DATMFS010000000
VERSION     DATMFS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35887"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..455)
                     /locus_tag="VNQ33_06395"
     CDS             complement(12..455)
                     /locus_tag="VNQ33_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97772.1"
                     /translation="MDLSRHTPGDNLIAGAGVLVLIDLLFLPWHQVFSFSSTAVDAPG
                     GWWGTLAVLLTVAILIVLVLRRHTAVSFTALPRPIGQLNLAANAAVLAALVIKLALDP
                     DFLGFGSYLGILLAAAMTAGALLGRDETDEPPPVGSGRGAPPTPF"
     gene            567..1133
                     /gene="purN"
                     /locus_tag="VNQ33_06400"
     CDS             567..1133
                     /gene="purN"
                     /locus_tag="VNQ33_06400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWJ97773.1"
                     /translation="MRVGVLVSGTGSILDAMIDAGVPVALVAADRLCRGLEIAAEAGI
                     ATELVERGRFDAGFDRDEYSTRLAGVLQAHALDLVAMAGFGTVLTQPMQDAFGGRLLN
                     THPALLPAFKGWHAVRDALAYGVKVTGCTVHVAGLDVDTGLILAQEAVAVLPGDTEES
                     LQERIKTVERRLYPATLLSIIEQGRVPA"
     gene            1183..>2602
                     /gene="purH"
                     /locus_tag="VNQ33_06405"
     CDS             1183..>2602
                     /gene="purH"
                     /locus_tag="VNQ33_06405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWJ97774.1"
                     /translation="MRALLSVYDKTGILELASGLHDLGWDLVSSGGTARSIRDAGIPV
                     ADTAEVTGFPAILGHRVVTLHPKIHGGILADRSNAHHVTEMADYGIEPFDLVVGNLYP
                     FGSDPAAFAHGGGTAEDVIDIGGPAMVRAAAKNHAHVGVLTDPSEYPAILSELRSSST
                     GTLSAETRRRLARAAFAHTAAYDAAVVAWFDEADDPVLPPTLHVAATREERTLRYGEN
                     PHQRAALYRTNGADPWWATATQYGGIALSYLNLYDADAAWLLAHDIGAASGDKPAVAI
                     IKHANPCGAAVADTLAAAYQQAFECDARSAFGGIVALNRVVDDETVVEMVAAAQADLV
                     IAPGYADGVIEALQGKRKNTRLIEAVAPVPATRHLRPISGGWLVQEPHRLVALPSEWE
                     VVTERVPTEAELADAALAFRVCAAVTSNSIVLVKDGVAWGIGAGQQNRVEAGQIAAEK
                     AAGRAQGGACASDAFYPFPDGIE"
BASE COUNT          373 a          920 c          955 g          354 t
ORIGIN      
        1 ggagcgttcg gtcagaacgg ggtcggtggt gcgccgcgtc ccgagcccac cggcggcggc
       61 tcgtcggtct cgtcgcggcc gaggagcgcg ccggccgtca tcgccgcggc gaggaggatg
      121 ccgaggtacg agccgaaccc caagaagtcg gggtcgagcg ccagcttgat cacgagggca
      181 gcgaggacgg cagcgttggc cgccaggttc agctgcccga tcgggcgggg cagggcggtg
      241 aacgagaccg cggtgtggcg gcggaggacg agcacgatca agatcgccac cgtgagcagg
      301 acggcgagcg tgccccacca gccccccggc gcgtcgaccg cggtgctcga gaacgagaag
      361 acctggtgcc acggcaggaa cagcaggtcg atcagcacga gcacgccagc gccggcgatc
      421 aggttgtctc ccggggtgtg ccggctcagg tccatgccgc tcctcgacgt ggacggtccg
      481 cggtggagcc aacgtaccgt ccgcgctcgc gtgcgggcag ctgcctcgga tcgggggctt
      541 cggagcggct ggtagcgtcc cggcacgtgc gagtgggcgt gttggtgtcg ggaacgggtt
      601 cgatcctcga tgccatgatc gacgccgggg ttccggtggc cctcgtcgcc gccgaccgtc
      661 tgtgccgcgg cctggagatc gccgcggagg cggggatcgc caccgaactc gtcgagcggg
      721 gccgcttcga cgccgggttc gaccgagacg agtactcgac ccgtctcgct ggcgtgctgc
      781 aggcccacgc gctcgacctg gtcgccatgg ccgggttcgg caccgtgctc acacagccga
      841 tgcaggacgc gttcggcggc cggctgctga acacgcaccc ggcgctgctg ccggcgttca
      901 agggctggca cgccgtgcgc gacgccctcg cctacggcgt gaaggtcacc ggctgcacgg
      961 tccacgtcgc aggcctcgac gtcgacaccg ggctgatcct cgcccaggag gcggtggccg
     1021 tgttgcccgg cgacacggag gaatcgctcc aggagcgcat caagaccgtc gagcgtcggc
     1081 tgtaccccgc cacgctcctc tcgatcatcg aacagggacg ggtcccggca tgacgctgtg
     1141 catgccgatc gccgttccac ctgctgaagg gaaccgcctg ccatgagggc gctgctgtcc
     1201 gtgtacgaca agaccgggat cttggagctc gcctccggcc tccacgacct cgggtgggac
     1261 ctcgtgtcca gcggtgggac ggcgcgctcc atccgcgatg ccggcatccc cgtggccgac
     1321 acggccgagg tcacgggctt cccggccatc ctcgggcacc gggtcgtgac cctccacccg
     1381 aagatccacg gcgggatcct cgccgaccgg tcgaacgcgc accacgtgac cgagatggcc
     1441 gactacggca tcgagccctt cgacctggtc gtcggcaacc tgtacccgtt cgggtcggat
     1501 cctgccgcgt tcgcccacgg cgggggcacc gctgaggacg tgatcgacat cggcggcccg
     1561 gcgatggtgc gggcggcggc gaagaaccac gcccacgtcg gcgtgctcac cgacccgtcg
     1621 gagtacccgg cgatcctgtc ggagctgcga tcgtcgtcga ccgggacgct gtcagccgag
     1681 acccgccgcc gactcgcccg ggccgccttc gcccacacgg cggcctacga cgccgctgtg
     1741 gtggcgtggt tcgacgaggc cgacgacccg gtcctgccgc cgacgctgca cgtcgccgcc
     1801 acccgggagg agcgcacgct gcgctacggc gagaacccgc accagcgggc cgcgctctac
     1861 cggacgaacg gcgccgaccc gtggtgggcc accgccacgc agtacggcgg catcgccctc
     1921 agctacctga acctgtacga cgccgatgct gcttggctgc tggcgcacga catcggtgcg
     1981 gcctccggcg acaagccggc ggtggcgatc atcaagcacg ccaacccgtg cggcgctgcc
     2041 gtggccgaca cgctcgcagc ggcctaccag caggccttcg agtgcgacgc ccgctcggcg
     2101 ttcggcggga tcgtggccct gaaccgggtc gtcgacgacg agacggtggt cgagatggtg
     2161 gccgcggccc aggccgacct ggtgatcgcc cccggctacg ccgacggcgt catcgaggcg
     2221 ctgcagggca agcggaagaa cacccggctc atcgaggccg tggctccggt cccagcgacg
     2281 cgccacctgc ggccgatctc cggcggttgg ctggtccagg agccgcaccg gctcgtggcg
     2341 ctcccgtcgg agtgggaggt cgtcaccgag cgggtgccca cggaggccga gctcgccgac
     2401 gcggcgctgg cgtttcgcgt gtgtgccgcg gtgacgtcga actcgatcgt gctcgtgaag
     2461 gacggggtgg cctggggcat cggcgccggg cagcagaacc gcgtcgaggc cggccagatc
     2521 gcagccgaga aggctgcggg tcgggcccaa ggcggtgcct gtgcgagcga cgcgttctac
     2581 ccgttccccg acggcatcga gg
//
LOCUS       DATMFS010000351         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_128627, whole genome shotgun sequence.
ACCESSION   DATMFS010000351 DATMFS010000000
VERSION     DATMFS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128627"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1394)
                     /gene="msrA"
                     /locus_tag="VNQ33_06410"
     CDS             complement(237..1394)
                     /gene="msrA"
                     /locus_tag="VNQ33_06410"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWJ97775.1"
                     /translation="MLFSRSKPETVTAATALAGHDEPTFDISGIHAVNGNSIVPPFPD
                     GTEIAVFAMGCFWGAERIFWRLPGVFATAVGYAGGFTRNPTYQETCTGRTGHTEAVLV
                     VYDPEQISYRRLLEVFFTEHDPTQVDGQGNDHGTQYRSALYVTTDEQLAEATSAFEVF
                     GKALAGAGKRPIATELAPAGEFFYAEEYHQQYLHKNPGGYCGLQGTGVVCQLPGPLAD
                     LGVDLDEDAAAKVADAAPVAHEEDPVTEDSKLKPLATTDEEWKQRLTPAQYQVLREAG
                     TERAFAGAYTDVEEPGLYRCMACGNPLYTSDTKFHSGSGWPSFTEAVSPGAVELIEDH
                     SHGMTRTEVRCGRCHSHLGHLFPDGPRDRGGLRYCMNSCALDLDQGAEPVR"
     gene            1574..2335
                     /gene="sufC"
                     /locus_tag="VNQ33_06415"
     CDS             1574..2335
                     /gene="sufC"
                     /locus_tag="VNQ33_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWJ97776.1"
                     /translation="MATPLFETQDLHVSADGNEILRGVSLCVGVGEIHALMGPNGSGK
                     STLASALLGSPEYEITSGRIRFKGDDITGWGPDDRGRSGLFLAFQYPQAIPGVSVINF
                     LRQALSARKGFDLSVLELRLAIMEWMERLDMDPSFADRYLNEGFSGGEKKRNEILQMA
                     ILEPELAILDETDSGLDIDALRVVAEGVREVRKDRPELGLLVVTHYQRLLDELQPTHV
                     HLLIDGRIVASGGMELAEKVEAEGFEAWQTAAAAS"
     gene            2346..>3072
                     /locus_tag="VNQ33_06420"
     CDS             2346..>3072
                     /locus_tag="VNQ33_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ97777.1"
                     /translation="MTALTTDPFDAAKIKTDFPLLQQEIKGRPIHFLDSAASSQKPQV
                     VIDAMTTYYETINANVHRGVYAIAEAATNALEASRIRVGRFIGAPNPETEVVFTKNAT
                     ESLNLVAQSWGRANLGPGDAVVLTQLEHHANIVPWFQLQAEKGFEIRWIPLTGDAQLD
                     LTDIDTLLDGAKILALTAMSNVTGTLTPIDELTRKAHDAGALVCLDACQYVPHLAIDL
                     GQMGVDFAAFSGHKMLGPTGIGVL"
BASE COUNT          454 a         1085 c         1062 g          471 t
ORIGIN      
        1 gccgcgaaca gctgctcggc ccgccgctgg gcatcgagga ggctcgtgcg gcggacgtcc
       61 tcgtcgtctt cagcagggat cctcgccatg acatcgagcg taggggcggc ggggagcctc
      121 ctgtctgctg ggtgcgggta caggcgtgcc ccggccgcgg gtggaggccg cgcgggtgtc
      181 ggcgtcggcg cgacgacggg gcgccgccgc aagcgacgcc ccgtcgaagg tgccggtcag
      241 cggaccggct cggcgccctg gtcgaggtcc agggcgcagc tgttcatgca gtagcggagg
      301 ccgccacggt cacggggacc gtcggggaag aggtggccga ggtggctgtg gcagcggccg
      361 cagcggacct cggtgcgggt catcccgtgc gagtggtcct cgatcagctc gacggcgccg
      421 ggcgacaccg cctcggtgaa cgagggccag ccgctgccgg agtggaactt ggtgtcgctc
      481 gtgtagagcg ggttcccgca cgccatgcag cggtagaggc cgggctcttc gacgtcggtg
      541 taggccccgg cgaacgcccg ctcggtgccc gcctcgcgga gcacctggta ctgggcgggg
      601 gtcagccgct gcttccactc ctcgtccgtg gtggcgaggg gcttgagctt gctgtcttcg
      661 gtcacggggt cctcctcatg ggcaacaggg gcggcatcgg ccaccttggc ggcggcgtcc
      721 tcgtcgaggt cgacgccgag gtcggcgagc gggccgggaa gctggcagac gacgccggtc
      781 ccctggaggc cgcagtagcc gccggggttc ttgtgcaggt actgctggtg gtactcctcg
      841 gcgtagaaga actcgccggc gggagccagc tcggtggcga tgggccgctt gccggcaccg
      901 gccagggcct tgccgaacac ctcgaacgcc gaggtggcct cggccagctg ctcgtcggtg
      961 gtcacgtaga gcgcggaccg gtactgggtg ccgtggtcgt tgccctgccc gtcgacctgg
     1021 gtggggtcgt gctcggtgaa gaacacctcg aggaggcgcc ggtacgagat ctgctcgggg
     1081 tcgtagacca cgagaacggc ctcggtgtgg ccggtccgac cggtgcaggt ctcctggtag
     1141 gtcgggttgc gggtgaagcc acccgcgtag ccgaccgccg tggcgaacac gccggggagc
     1201 cgccagaaga tgcgctcggc accccagaag caccccatcg cgaacacggc gatctcggtc
     1261 ccgtccggga acggcggcac gatcgagttg ccgttgacgg cgtggatgcc gctgatgtcg
     1321 aacgtgggct cgtcgtggcc ggccagcgcc gtcgccgccg tgaccgtctc gggcttgctg
     1381 cggctgaaca acatgggtgg gtacctcctg ggcacctcgg tgggcgtcgg gtgacgcctc
     1441 ggacagacct gtcaacagcg tggcgctccc gtttggtccc gtccacctgg ccgatctgcg
     1501 gatccgggcg gcgccgcgga ttttctccct ccggcattac cactatcgcc gtaggactaa
     1561 tacactggcg cccatggcaa cgcccctgtt cgagacccag gacctgcacg tcagcgccga
     1621 cggcaacgag atccttcgag gggtcagcct ctgtgtgggc gtcggcgaga tccacgccct
     1681 catgggaccc aacggctccg gcaagtccac cctcgccagc gccctcctcg gcagccccga
     1741 gtacgagatc acctcgggcc gcatccgctt caagggcgac gacatcaccg gctggggccc
     1801 cgacgaccgc ggccgcagcg gcctgttcct cgccttccag tacccccagg ccatccccgg
     1861 cgtgtcggtc atcaacttct tgcgccaggc cctctcggcc cgcaaggggt tcgacctctc
     1921 cgtcctggag ctgcgcctgg cgatcatgga gtggatggag cggctggaca tggacccgtc
     1981 gttcgccgac cgctacttga acgagggctt ctccggcggc gagaagaagc gcaacgagat
     2041 cctgcagatg gccatcctcg agccggagct ggcgatcctc gacgagaccg actccggcct
     2101 cgacatcgac gccctgcgcg tcgtggccga gggggtgcgg gaggtccgca aggaccgccc
     2161 cgagctcggc ctgctggtcg tcacccacta ccagcgcctg ctcgacgagc tgcagcccac
     2221 ccacgtgcac ctgctcatcg acggccgcat cgtcgcttcc ggtggcatgg agctggccga
     2281 gaaggtggag gccgagggct tcgaggcgtg gcagaccgcc gcagctgcgt catgatgagc
     2341 cggccatgac cgccctcacg accgacccgt tcgacgccgc caagatcaag accgacttcc
     2401 cgctgctcca gcaggagatc aagggccggc ccatccactt cttggactcg gcggcctcgt
     2461 cgcagaagcc gcaggtcgtc atcgatgcga tgacgaccta ctacgagacc atcaacgcca
     2521 acgtccaccg gggcgtctac gccatcgccg aggctgccac caacgccctc gaggcgtctc
     2581 gcatccgggt gggccggttc atcggggcgc ccaaccccga gaccgaggtc gtcttcacca
     2641 agaacgccac cgagtcgctg aacctggtgg cccagtcgtg gggccgggcc aacctcggcc
     2701 ccggcgacgc cgtggtgctc acccagctcg agcaccacgc caacatcgtc ccgtggttcc
     2761 agctccaggc cgagaagggc ttcgagatcc gctggatccc gctcaccggc gacgcccagc
     2821 tggacctcac cgacatcgac acgctgctcg acggggccaa gatcctggcc ctcaccgcca
     2881 tgtcgaacgt gacgggcacg ctcacgccga tcgacgagct cacccgcaag gcccacgacg
     2941 ccggcgccct cgtctgcctc gacgcctgcc agtacgtgcc ccacctcgcc atcgacctgg
     3001 gccagatggg cgtcgacttc gccgccttct ccgggcacaa gatgctcggt cccaccggca
     3061 tcggcgtgct ct
//
LOCUS       DATMFS010000352         4609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_58144, whole genome shotgun sequence.
ACCESSION   DATMFS010000352 DATMFS010000000
VERSION     DATMFS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58144"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..972)
                     /locus_tag="VNQ33_06425"
     CDS             complement(136..972)
                     /locus_tag="VNQ33_06425"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWJ97778.1"
                     /translation="MGGVQSAKVAVRRVQKRARKTSQRMRGDLRTRTGRYRPLPDFLV
                     IGTQKGGTTSLHDYLAAHPRTAPSNVKEVHYFDTELRPPLDWYRGHFPLRPGDRKVFE
                     TTPRYLFHPDAAERIHAVLPEVKMIALLRNPVERALSNYRMSSRGVREDLEMLEAFEA
                     EEERTAAPRSAEKANADRDWYAYKARGRYAEQLTRYFDRFGREQVLVVRSEDLFEDSA
                     ATCREILQFLELPDGPPSLQLPRSNKTKEADVPSAARRLLEDYYAGPNEELAELLGRR
                     LW"
     gene            1133..2560
                     /locus_tag="VNQ33_06430"
     CDS             1133..2560
                     /locus_tag="VNQ33_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWJ97779.1"
                     /translation="MSPTRREFLAGALAAAGAVTIAGCSNDSSSDGGAASTTTRPKAS
                     PPESSIARVSELGDPARSGLDHIIVVMMENRSFDHYLGWLPGADGKQSGLTFVDQHGT
                     SFDTHRLTDFQNCAHPDPDHSFDGGRIQLADGACDGFLRSGDNDEFAIGYYTNEDLPF
                     TGKVASHWCALDRYFAATMAGTYPNRFYQHSAMTDRTHNSFDIAAMPTIWDRLAAKGV
                     SARYYYSDVPFIALYGGKYTSISQPFDAFLQDAEKGTLPAVSFIDPKFTDEGAGTSAD
                     DHPHADIRAGQWFLDQVYRAVTLGPKWDKTLVVVNYDEWGGFYDHVAPAEAPDATPAA
                     GTGLRGFRVPTMMFGPSVRRGTIGHETYDHTSILKMIEWRHDLASLTPRDKAARNLAE
                     ILDLSAKPDTDAPQWNVAAFDPTACPPEPEDEPATTTTTMSAPAAGSGEGASGPTRAG
                     EDHYAEWRGIRTLADRHRFPGGFGF"
     gene            complement(2621..3688)
                     /locus_tag="VNQ33_06435"
     CDS             complement(2621..3688)
                     /locus_tag="VNQ33_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97780.1"
                     /translation="MAVSKRRSSVLRAGALAACMVAGVVVMVAAPATADPTNYTVTKI
                     NGTVVDTGIPTTCHQDGTGPGSNVNGHWLNVPMDYSATSSELDCAHAVADFMLYLARY
                     EDEFSASVPQALRSSVLSGTVNLSSPYEVFLDNIYVTTSALATLDTSAPGSGTGLGSC
                     GFVDYMLLVRCSDDALGTVILRDDWGNPYRDWAGNPISFNLNGLLSQRGGTTYGNLFL
                     TNDDAYGLQSFDDRTLHHEQVHSQQWSQWGWDFARRYIQDYVDSAGNANYNYWLYGPT
                     QGVQVPAQCYQQFEKAAGFQDGGYAYRDPLHGGPADATEASAWGTQFVPNGQAYPTGW
                     TTSQNNAAGGKCPSTWQATTA"
     gene            complement(3931..4004)
                     /locus_tag="VNQ33_06440"
     tRNA            complement(3931..4004)
                     /locus_tag="VNQ33_06440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3968..3970),aa:Val,seq:tac)
     gene            complement(4069..4515)
                     /locus_tag="VNQ33_06445"
     CDS             complement(4069..4515)
                     /locus_tag="VNQ33_06445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ97781.1"
                     /translation="MSTPEASAPSPRGAEGGIPLHNLLGFEVHPEPDGSISRVDMPVR
                     AESFGVNDNLHGGALATLVDLTSALAAVRHRTIDLETESLVTSDIHIRYLGQPRTDTV
                     TGSAEVVRDGRQMIVIDCKVHDAGGHLIATADVGMMVVTRRRPLPS"
BASE COUNT          688 a         1629 c         1591 g          701 t
ORIGIN      
        1 acgcgcatca acggtcgagc cgcatcgaac gatcgcaccg ggcgccgacg gccagcccgt
       61 gacagctcga gtggcggatc tgggcgcggt ccatcggtga aagtctacgc gaccgctgga
      121 gaaacgcctg gaccgctacc agagccggcg gccgagcagc tcggcgagct cctcgttggg
      181 gccggcgtag tagtcctcga ggagccggcg cgccgcggat gggacgtcgg cctccttcgt
      241 cttgttgctg cggggcagct gcaggctcgg tggtccgtcg ggcagctcga ggaactgcag
      301 gatctcgcgg cacgtggctg ccgagtcctc gaagaggtct tccgagcgca cgaccagcac
      361 ctgctcccgg ccgaagcggt cgaagtagcg ggtcagctgc tcggcgtagc gcccgcgggc
      421 cttgtacgcg taccagtcgc ggtcggcgtt ggccttctct gccgaccggg gtgcggcggt
      481 gcgctcctcc tccgcctcga acgcctccag catctcgagg tcctcgcgca cgccgcgcga
      541 gctcatccgg tagttcgaca gcgcccgctc gacggggttc cggagcaggg cgatcatctt
      601 cacctcgggg aggacggcgt ggatccgctc ggccgcgtcg gggtggaaga ggtagcgagg
      661 cgtggtctcg aacaccttcc ggtcgcctgg gcgcaacggg aagtggcccc ggtaccagtc
      721 cagcggcggc cgcagctcgg tgtcgaagta gtggacctcc ttgacgttgc tcggtgccgt
      781 gcgcggatgg gcggcgaggt agtcgtggag gctggtggtg ccgcccttct gggtcccgat
      841 gaccaagaag tccggcagcg gccggtagcg cccggtgcgc gtccgcaggt cgccgcgcat
      901 ccgctgcgag gtcttccgag cgcgcttctg cacgcgccgc accgccacct tcgccgactg
      961 gactcctccc atggacgctt tctcccacag atcgcgcacg ggcgcaaagc cgtcgtgcgg
     1021 gtgcggctcc cgggcggccc tcgggcgggg gcgcacggtg cggcgcgggg gtacggcggg
     1081 tgaacctacg atcggcgccg gccacgatcg ccacctgaga ggtgctttgc cgatgtctcc
     1141 gacccgccga gagttcctgg ccggcgcgct ggcggcggct ggtgccgtca ccatcgccgg
     1201 ctgctccaac gactcgtcct ccgatggcgg cgccgcctcc accacgaccc ggcccaaggc
     1261 ctcgccaccc gagtcgtcga tcgcccgggt ctccgagctc ggtgacccgg cccgctccgg
     1321 gctcgaccac atcatcgtcg tgatgatgga gaaccggtcc ttcgaccact acctcggctg
     1381 gctgccgggc gcggacggca agcagtcggg gctgacgttc gtcgaccagc acggcacctc
     1441 cttcgacacg caccggctca ccgacttcca gaactgcgcc caccccgacc ccgaccacag
     1501 cttcgacggc ggacgcatcc aactcgccga cggcgcgtgc gacggcttct tgcgttcggg
     1561 cgacaacgac gagttcgcca tcggctacta cacgaacgag gacctgccgt tcaccggcaa
     1621 ggtcgcctcg cactggtgcg cgctcgaccg gtacttcgcc gccaccatgg cggggaccta
     1681 cccgaaccgc ttctaccagc acagtgcgat gacggaccgg acgcacaact cgttcgacat
     1741 cgccgcgatg cccacgatct gggaccgcct ggcggccaag ggcgtgagcg cccgctacta
     1801 ctactccgac gtgccgttca tcgccctcta cggcggcaag tacacgtcga tctcccagcc
     1861 gttcgacgcc ttcctccaag acgccgagaa gggcacgctg cctgcggtgt cgttcatcga
     1921 cccgaagttc accgacgagg gcgccggcac ctccgccgac gaccatccgc acgcggacat
     1981 ccgagccggc cagtggttcc tcgaccaggt gtaccgggcg gtcaccttgg ggccgaagtg
     2041 ggacaagacg ctggtggtcg tgaactacga cgaatggggc gggttctacg accacgtcgc
     2101 cccggccgag gcacccgacg ccaccccagc ggccggcacc ggcctgcggg ggttccgggt
     2161 gccgacgatg atgttcggtc catccgtgcg gcgcggcacg atcggccacg agacctacga
     2221 ccacacctcg atcctgaaga tgatcgagtg gcgccacgac ctcgcctcgc tgacgccgag
     2281 ggacaaggcg gcccgcaacc tggccgagat cctcgacctc agcgccaagc ccgacaccga
     2341 cgccccgcag tggaacgtcg ccgccttcga cccgacagcc tgcccgcccg aaccagaaga
     2401 cgagccggcg acgaccacca ccacgatgtc cgccccggcc gcgggctccg gcgaaggggc
     2461 gagcggcccg acccgcgccg gtgaggacca ctacgccgaa tggcgcggca tccgcacgct
     2521 ggccgaccgc caccgcttcc ccggcggctt cggcttctga ccgctggcac ctcgccgtcc
     2581 gctgccctca ggaccgcaga cggcgggtgc catggacccc tcaggccgtg gtggcctgcc
     2641 aggtcgacgg gcacttgccg ccggccgcgt tgttctggct ggtggtccag ccggtcgggt
     2701 aggcctggcc gttggggacg aactgggtgc cccatgccga ggcctcggtg gcatcggccg
     2761 ggccgccgtg gagcggatcc cggtaggcgt agccgccgtc ttggaagccg gccgccttct
     2821 cgaactgctg gtagcactgc gccgggacct ggacgccctg ggtcgggccg tagagccagt
     2881 agttgtagtt cgcgttgccc gccgagtcga cgtagtcctg gatgtagcga cgggcgaagt
     2941 cccagcccca ctgcgaccac tgctgcgagt ggacctgctc gtggtggagg gtccggtcgt
     3001 cgaaggactg caggccgtag gcgtcgtcgt tggtcaagaa caggttcccg taggtcgtcc
     3061 cgcctcgctg gctgagcagg ccgttgaggt tgaacgagat cgggttgccc gcccagtccc
     3121 ggtacgggtt gccccagtcg tcgcgcagga tgaccgtgcc cagcgcgtcg tccgagcacc
     3181 gcaccaagag catgtagtcc acgaagccgc agctccccag gccggtcccc gatcccggcg
     3241 ccgacgtgtc gagcgtcgcc agcgccgagg tggtgacgta gatgttgtcg aggaacacct
     3301 cgtaggggct gctcaggttc accgtgccgc tcaggaccga actgcgcagc gcctgcggga
     3361 ccgacgcgct gaactcgtcc tcgtagcggg cgaggtacag catgaagtcc gcgaccgcat
     3421 gagcgcagtc cagctccgac gaggtcgccg agtagtccat cggcacgttc agccagtggc
     3481 cgttgacgtt cgagcccggc ccggtgccgt cctggtggca ggtggtcggg atccccgtgt
     3541 ccacaaccgt gccgttgatc ttcgtcaccg tgtagttcgt cggatcagcc gttgccggag
     3601 cagcgaccat cacgaccacg ccggcgacca tgcaagccgc caacgcaccg gcgcgcagca
     3661 cggagctgcg cctcttcgat accgccatgg gttcccccct ttgtccgccg ccgcccaccg
     3721 tgaacggcgt gcagcaggca cggtaatccc gaacggcgag acgtgctggc caaacggccg
     3781 accggcgatc aggccatcgg ccgtcgcctc cgtctgctgc gaccacgttt gcgactggtc
     3841 gtccaggctc agccgcacag gggaccgatg acctcggctg ggagatggcg tggagcccgg
     3901 gagcacgatc accgatcaga cgattcggag tgggcgcaga gggactcgaa cccccgacat
     3961 ctgccttgta agggcagcgc tctagccaac tgagctatgc gcccggaacg tgggcacgct
     4021 acttggcctc gctcccctgc ctctacggcg ttgagccccg gccgcggatc agctggggag
     4081 ggggcggcgc cttgtgacga ccatcatgcc gacatccgcg gttgcgatga ggtggccgcc
     4141 ggcgtcgtgg accttgcagt cgatgacgat catctggcgg ccatcgcgca cgacctcggc
     4201 gctgccggtg accgtgtcgg tcctcggctg gccgaggtag cggatgtgga tgtcgctggt
     4261 gacgagggac tcggtctcga ggtcgatcgt gcggtggcgc acggcggcca gggccgacgt
     4321 caggtccacg agggtggcca gggcgccgcc gtggaggttg tcgttgacgc cgaacgactc
     4381 ggcccgcacg ggcatgtcga cgcggctgat cgagccatcg ggctcggggt ggacctcgaa
     4441 gccgaggagg ttgtgcagcg ggatgccgcc ctcggcgccg cggggtgacg gcgccgacgc
     4501 ctccggtgtg ctcacgacgc ccgctcgcgg atggtgcagc tgatcggcag cgagtccagt
     4561 ccccggatga acagccggcc gccccacatc gggtcgccca cgagctgcg
//
LOCUS       DATMFS010000353         4017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_128720, whole genome shotgun sequence.
ACCESSION   DATMFS010000353 DATMFS010000000
VERSION     DATMFS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128720"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VNQ33_06450"
     CDS             complement(<1..620)
                     /locus_tag="VNQ33_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWJ97782.1"
                     /translation="MHAGLVIELQRGRDGSVAARGDLAVAPYWCRWDGTVLWIVGSAA
                     TPVGDDDITIDLQVGEGVHATVRSVAATIVYAARGPGTRLTTRLRVAAGASLAWQPDP
                     VIVTERARHRSALVADVATGGSLVADEVVVFGRSGERAGRFASSTDLRRDGLPVTVTS
                     FDTAMPGWSGPGGTDGAKVVATRLVLGPGSGIGDGATGHVDGRTVVL"
     gene            complement(622..1299)
                     /gene="ureG"
                     /locus_tag="VNQ33_06455"
     CDS             complement(622..1299)
                     /gene="ureG"
                     /locus_tag="VNQ33_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWJ97783.1"
                     /translation="MHLPHHTHHNEPPRRSPAHGGAFRVGIGGPVGTGKTALVAALCG
                     LLADGLRIGVVTNDIYTTEDADTLRRRGVLPAERIGAVQTGCCPHTAIRDDITANLDA
                     IEDMEQREGPLDLVLLESGGDNLTLTFSTGLIDVQLFVLDVAGGDKVPRKGGPGVTRS
                     DLLVINKTDLAPLVGADLDVMARDSEAQRGELPVAFISLVDPSGAQPVADWIVHELAR
                     WRADAVA"
     gene            complement(1301..1987)
                     /locus_tag="VNQ33_06460"
     CDS             complement(1301..1987)
                     /locus_tag="VNQ33_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWJ97784.1"
                     /translation="MAAAGTTFSSDASAASALLLLADGRFPDGSHAHSFGMEAAVTAG
                     RVHDPGSLGRYIEVRLWTTARTDAAAAVLAARGGDRLDAVDDALTVRLPSSSARQASR
                     SLGRSLVRTAARLWPGQEPRLADGRPPLQPIALGAVATLSGCGPQEAALCVTHGAAAA
                     LSSAALRLLGLDPFEVAGVLADLRGAVAQVAESVQETAGPSDLPTASTPLAELDAELQ
                     RTIEPRLFGS"
     gene            complement(1987..3720)
                     /locus_tag="VNQ33_06465"
     CDS             complement(1987..3720)
                     /locus_tag="VNQ33_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWJ97785.1"
                     /translation="MTGRAVTMDRARYAALYGPTVGDRIRLADTDLLIQIEADVSGGP
                     GRAGEEAVFGGGKVIRESMGQSTRTAAAGAPDLVITGAVVLDHWGIVKADVGVRDGRI
                     VALGKAGNPDTMDGVHPDLVIGPGTEVIAGNGKILTAGAIDSHVHLICPQVMDTALAT
                     GITTIIGGGTGPAEGTKATTVTAAPWYLEQMLTSLDAWPINVALLGKGNTVSSESLWE
                     QLRAGASGFKLHEDWGSTPAAISACLDVCDEAGVQAALHTDTLNEAGFVETTLAAIAG
                     RSIHAYHTEGAGGGHAPDIITVAGQANVLPSSTNPTRPHTVNTVDEHLDMLIVCHHLN
                     PSVPEDLAFAESRIRPSTIAAEDVLHDLGAISMIGSDSQAMGRVGEVVIRTWQTAHAM
                     KRRRGPLEGDTGADNTRAKRYVAKYTICPAIAHGLDGEVGSVEVGKLADLVLYDPRFF
                     GVRPHLVVKGGMISMAQMGDANASIPTPQPVMARPMFAAVGATAGRTSVAWVAPAAID
                     DGLGERLGLARDLVAVKDCRSVAKAHMVNNAATPEIRVDPDSFEVWIDGEAIEQSPVE
                     ELPMAQRYFLF"
     gene            complement(3717..>4017)
                     /gene="ureB"
                     /locus_tag="VNQ33_06470"
     CDS             complement(3717..>4017)
                     /gene="ureB"
                     /locus_tag="VNQ33_06470"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWJ97786.1"
                     /translation="GAEPIVLFEGRELTELLVVNDGDRPVQVGSHFHFAEANEALSFD
                     RRAATGRRLAVPAGTSVRFEPGVPMTVLLVDLAGERVAAGFRGLHGGAVTPGGAP"
BASE COUNT          518 a         1457 c         1509 g          533 t
ORIGIN      
        1 agcaccaccg tgcggccgtc gacgtggccg gtcgcgccgt cgccgatccc cgatcccggc
       61 ccgagcacca agcgggtcgc gacgaccttg gcgccgtcgg tgccgccggg gccggaccac
      121 ccgggcatcg ccgtgtcgaa cgaggtcacg gtgacgggga gcccgtcgcg ccggaggtcg
      181 gtcgacgagg cgaaccggcc ggccctctcc ccgctccgcc cgaacaccac cacctcgtcg
      241 gccaccaggg agcctccggt ggccacgtcg gccacgagcg cggaccggtg acgcgcccgc
      301 tcggtgacga tcacgggatc gggctgccag gccagcgacg caccagcggc gacgcggagg
      361 cgggtcgtga ggcgcgtccc cggtccgcgg gcggcgtaga cgatcgtggc ggcgacgctc
      421 cggacggtgg cgtggacgcc ctcgcccacc tggaggtcga tcgtgatgtc gtcgtcgccc
      481 accggtgtgg ccgccgaccc gacgatccag aggaccgtgc cgtcccaccg gcaccagtac
      541 ggcgccacgg ccaggtcccc gcgagcagcc acggagccgt cacggccgcg ctgcagctcg
      601 atgacgagcc cggcgtgcac ctcaggcgac cgcgtcggcg cgccagcggg ccagctcgtg
      661 cacgatccag tcggccacgg gctgggcgcc ggagggatcg accagcgaga tgaacgccac
      721 cggcagctcg ccccgctggg cctccgagtc gcgggccatg acgtcgaggt cggcccccac
      781 cagcggcgcc aggtcggtct tgttgatgac cagcaggtcc gaacgggtca caccaggccc
      841 gcccttgcgg ggcaccttgt cgcccccggc cacgtcgagc acgaagagct ggacgtcgat
      901 caggccggtg gagaaggtga gcgtgaggtt gtcgccgccg ctctcgagca gcacgaggtc
      961 gagcggaccc tcccgctgct ccatgtcctc gatggcgtcg aggttggcgg tgatgtcgtc
     1021 gcggatggcg gtgtgcgggc agcacccggt ctgcacggcg ccgatgcgct cggcgggcag
     1081 cacgccgcgg cggcgcaggg tgtcggcatc ctcggtcgtg tagatgtcgt tggtcaccac
     1141 accgatgcgc aggccgtcgg cgagcagccc gcacagcgcg gccacgagcg cggtcttgcc
     1201 ggtgccgacc ggcccgccga tgccgacgcg gaacgccccg ccgtgggccg ggctccggcg
     1261 cggcggctcg ttgtggtggg tgtggtgcgg caggtgcatg tcagctcccg aagagtcgcg
     1321 gttcgatggt gcgctggagc tcggcgtcca gctcagccag cggcgtggac gccgtcggca
     1381 ggtcgctggg gcccgcggtc tcctgcacgg actcggccac ctgggcgacc gcgccccgca
     1441 ggtcggccag gaccccggcg acctcgaacg ggtcgagccc gaggagccgc agcgccgcgc
     1501 tcgacagcgc cgccgccgcg ccgtgggtga cgcagagcgc ggcctcctgg gggccgcagc
     1561 ccgacagggt ggcgacggca ccgagggcga tcggttgcag cggcgggcgg ccgtcggcga
     1621 gccgaggctc ctgtcccggc cacagccggg cggcggtgcg gaccagtgag cggccgagcg
     1681 agcgcgacgc ctgccgggcg gagctgctcg gcaggcgcac ggtgagggcg tcgtccaccg
     1741 catcgagccg atcaccgcct cgcgccgcga ggacggcggc ggcggcgtcg gtgcgcgccg
     1801 tggtccacag gcggacctcg atgtagcgcc cgagcgagcc cgggtcgtgc acacggcccg
     1861 ccgtcacggc cgcctccatg ccgaagctgt gggcgtggct gccgtcgggg aaccggccgt
     1921 cggccagcag gagcagcgcc gacgccgcgc tcgcatcgct cgagaaggtc gtgccggcgg
     1981 cggccatcag aacaggaagt agcgctgggc catcgggagc tcttcgaccg gcgactgctc
     2041 gatcgcctcg ccgtcgatcc acacctcgaa ggagtcggga tccacccgga tctcaggtgt
     2101 ggccgcgttg ttgaccatgt gcgccttggc cacggaccgg cagtccttca ccgccaccag
     2161 atcgcgagcc aggccgagcc gctcgccgag gccgtcgtcg atcgctgccg gcgcgaccca
     2221 cgccaccgag gtccgtccgg cggtggcccc caccgctgcg aacatgggcc gggccatgac
     2281 cggttgcggc gtggggatcg atgcgttggc gtcgcccatc tgggccatgg agatcatccc
     2341 gcccttgacc acgaggtgcg gacggacgcc gaagaagcgc gggtcgtaga gcacgaggtc
     2401 ggcgagcttc cccacctcga ccgagccgac ctcgccgtcg agcccgtggg cgatcgccgg
     2461 gcagatcgtg tacttggcca cgtagcgctt ggcgcgggtg ttgtcggcgc cggtgtcgcc
     2521 ctcgagcggg ccgcggcggc gcttcatggc gtgggcggtc tgccacgtgc ggatgaccac
     2581 ctcgccgaca cgccccattg cctgcgagtc cgagccgatc atcgagatgg cgccgaggtc
     2641 gtgcagcacg tcttcggcgg cgatggtcga cggccggatg cggctctcgg cgaacgccag
     2701 gtcctcgggc accgaggggt tcaggtggtg gcagacgatg agcatgtcga ggtgctcgtc
     2761 gaccgtgttc accgtgtggg gccgggtcgg gttggtcgac gacggcagca cgttcgcctg
     2821 gcccgcgacc gtgatgatgt ccggcgcgtg gccgccgccc gccccctccg tgtggtaggc
     2881 gtggatcgag cggccggcga tggccgcgag cgtggtctcc acgaagccgg cctcgttcag
     2941 ggtgtcggtg tggagggcgg cctggacacc cgcctcgtcg cacacgtcga ggcacgccga
     3001 gatggcggcg ggcgtcgagc cccagtcctc gtggagcttg aacccgctgg cgcccgcccg
     3061 aagctgctcc cagagggact cgctcgacac cgtgttgccc ttgcccagga gggcgacgtt
     3121 gatgggccag gcgtcgagcg aggtgagcat ctgctcgagg taccagggcg ccgcggtcac
     3181 ggtcgtcgcc ttggtcccct cggccggacc cgtcccgccg ccgatgatcg tggtgatgcc
     3241 ggtcgccagg gcggtgtcca tgacctgcgg gcagatgagg tgcacgtggg agtcgatggc
     3301 cccggccgtg aggatcttcc cgttgccggc gatgacctcg gtccccgggc cgatcaccag
     3361 gtcggggtgg acgccgtcca tcgtgtcggg gttgccggcc ttgcccagcg ccacgatgcg
     3421 cccgtcgcgc acgccgacgt cggccttcac gatgccccag tggtccagga ccacggcgcc
     3481 ggtgatgacc aggtccggtg cgcccgccgc cgccgtcctc gtggactggc ccatcgactc
     3541 gcggatgacc ttgccgccgc cgaagaccgc ctcctcgccc gcccggccag gcccgccgct
     3601 cacgtcggcc tcgatctgga tgagcaggtc ggtgtcggcc aggcggatgc ggtcgcccac
     3661 cgtcggtccg tacagcgcgg cgtagcgcgc ccggtccatc gtcacggcgc ggccggtcac
     3721 ggcgcacctc ccggggtgac ggccccgccg tggaggccgc ggaacccggc ggccacccgc
     3781 tcaccggcga ggtcgacgag cagcacggtc atcggtacgc cgggctcgaa gcgcacgctg
     3841 gtgcccgccg gcaccgccag ccgccgcccg gtggcggcgc ggcggtcgaa cgacagcgcc
     3901 tcgttcgctt cggcgaagtg gaagtgcgag ccgacctgga cgggccggtc gccgtcgttc
     3961 accaccagga gctcggtcag ctcccggcct tcgaagagca cgatcggctc ggccccc
//
LOCUS       DATMFS010000354         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_58204, whole genome shotgun sequence.
ACCESSION   DATMFS010000354 DATMFS010000000
VERSION     DATMFS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58204"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VNQ33_06475"
     CDS             <1..883
                     /locus_tag="VNQ33_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97787.1"
                     /translation="ALGSLGTGRREAGDLAGSAQAHQEELAVASQLGDPAAVATAQVN
                     LGNVAVAGNDLQNGLAWYDAAEPALRERQLWMALVPLLNNRWQVHAALGDDTSATDDL
                     VACGRACVLSGAWQQAAQILPQALQHLNGTGRPAEAGPVYEDLAATARALGDDSMLQQ
                     ALGDRALLVLGTGDLAGATALLDQQDAICRRTGNQAGLAACIGNRAIVKQQQGDLTGA
                     LGCVDEQLQLSQATGSAQGILFATANRGELLGQLGRRQEALAALAQARQMAAQYGLAP
                     MVQQLDQMIAAVDASQN"
     gene            complement(970..1467)
                     /locus_tag="VNQ33_06480"
     CDS             complement(970..1467)
                     /locus_tag="VNQ33_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWJ97788.1"
                     /translation="MGTNQRSQIEMTHDEIVQYLERSRTATMVTNGPSGHPHAVAMWY
                     AVVDGAVWFETKAKSQKAANLRRDPKITVLIEDGHTYDTLRGVSLEGTAEIIDDADAL
                     WAVGVSIWERCNGPYTDEVKPLVEFMLNKRVAVRVDVTRTRSWDHRKLGMDPIPLGGT
                     TAQYL"
     gene            complement(1506..>2335)
                     /locus_tag="VNQ33_06485"
     CDS             complement(1506..>2335)
                     /locus_tag="VNQ33_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HWJ97789.1"
                     /translation="VDPAARQKWEALRLLHPALDSVCEPTLVDLDLQAPEPEEVDGFL
                     ALLDEDPPTWIAVADADETVALANTGFLHQHLQGQVPIVVRMQSESGLGGLIQPQPGR
                     APTLPGVELFPFLDRTCTVETVDGGVREQLAESVHEDYLAHLDPGAPVSSLARPWSEL
                     SDADREQSRKRVDGIVADLDALGLDLVPLRRWGAPEVTLTDGEVDALAARDHQRWFDD
                     RTTAGWTYGEVRDDETKQNPLLVSWGDLPDDAKAFNLATARELLPMLARNGFEAAAR"
BASE COUNT          330 a          835 c          847 g          323 t
ORIGIN      
        1 cgccctcggc tcgctcggca ccgggcggcg cgaggccggt gacctggccg gttcggccca
       61 ggcccaccag gaggagctgg ccgtcgcctc ccagctcggc gaccccgccg ccgtcgccac
      121 cgcccaggtc aacctgggca acgtcgccgt cgccggcaac gacctccaga acgggctggc
      181 ctggtacgac gcagccgagc cggcgctgcg ggagcggcag ctctggatgg ccctggtccc
      241 gttgctcaac aaccggtggc aggtccacgc cgccctcggc gacgacacga gcgccaccga
      301 cgacctcgtc gcctgcggcc gcgcctgcgt cttgtcgggc gcgtggcaac aagccgccca
      361 gatcctcccc caagccctcc agcacctgaa cgggaccggc cggcccgcgg aggccggacc
      421 ggtctacgag gacctggcgg ccacagcccg ggcgctcggc gacgacagca tgctccagca
      481 ggccctgggc gaccgggccc tgctcgtgct cggcaccggc gacctggccg gtgccaccgc
      541 cctgctcgac cagcaagacg ccatctgccg tcgcaccggc aaccaggccg gcctggccgc
      601 ctgcatcggc aaccgggcca tcgtgaagca gcagcagggc gacctcaccg gcgcccttgg
      661 ctgcgtcgac gagcagctcc agctcagcca ggccaccggc agcgcccagg gcatcctgtt
      721 cgccaccgcc aaccgaggcg agctgctggg tcagctcggc cgcaggcagg aggccctggc
      781 cgcgctggcc caggcccgcc agatggccgc ccagtacggc ctcgccccca tggtccagca
      841 gctcgaccag atgatcgccg ccgtcgacgc ctcccagaac tgatcagaga gccaccccga
      901 actgatccga ccgctcgatg cggatggtcg cggtgcaacc acgaacgccg cgtgttcgag
      961 aagcggtcgt cagaggtact gggcggtggt gccgccgagg gggatggggt ccatgccgag
     1021 cttgcggtgg tcccagctgc gcgtgcgggt gacatcgacc cggaccgcca cccgcttgtt
     1081 gagcatgaac tcgacgagcg gcttcacctc gtcggtgtag gggccgttgc agcgctccca
     1141 gatgctcacg cccacagccc acagggcatc ggcgtcgtcg atgatctcgg ccgtgccctc
     1201 gagcgacaca ccgcggaggg tgtcgtaggt gtggccgtct tcgatcagca cggtgatctt
     1261 cgggtcccgg cggaggttgg cggccttctg cgacttggcc ttggtctcga accagacggc
     1321 cccgtcgacg acggcgtacc acatggccac ggcgtgagga tggccgctcg ggccgttggt
     1381 gaccatggtc gcggtgcggc tccgctccag gtactggacg atctcgtcgt gggtcatctc
     1441 gatctggctg cgctggttcg tccccatggg ccgggaccct aacccggcgg gcaccgctga
     1501 tctgatcagc gggcggcggc ttcgaagccg ttgcgggcga gcatcgggag cagctcgcgg
     1561 gcggtggcca ggttgaacgc cttggcgtcg tcggggaggt cgccccacga gacgagcagc
     1621 gggttctgct tggtctcgtc gtcgcggacc tccccgtagg tccagccggc ggtggtgcgg
     1681 tcgtcgaacc agcgctggtg gtcccgggcg gcgagggcat cgacctcgcc gtcggtcagg
     1741 gtgacttcgg gcgcacccca gcgccggagg gggaccagat cgaggccgag ggcgtcgaga
     1801 tcagcgacga tgccgtccac ccgcttgcgg ctctgctcgc ggtcggcgtc ggagagctcg
     1861 ctccagggcc gggcgagcga ggagaccggc gcgcccgggt cgaggtgggc gaggtagtcc
     1921 tcgtggaccg actcggcgag ctgctcacgg acgcccccgt cgacggtctc gaccgtgcag
     1981 gtccgatcga ggaacgggaa cagctcgacc cccggcaggg tcggggcgcg accgggctgg
     2041 ggctggatga gcccgccgag gccggactcg ctctgcatgc ggaccacgat cgggacctgc
     2101 ccctgcaggt gctggtgcaa gaacccggtg ttggccaggg cgacggtctc gtcggcgtca
     2161 gccaccgcga tccaggtggg cggatcctcg tcgagcaggg cgaggaagcc gtcgacctcc
     2221 tccggctcgg gtgcctggag gtcgaggtcg acgagggtcg gctcgcacac cgagtcgagc
     2281 gccgggtgga ggaggcggag cgcctcccac ttctgccgag ccgcgggatc gacca
//
LOCUS       DATMFS010000355         9361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_198538, whole genome shotgun sequence.
ACCESSION   DATMFS010000355 DATMFS010000000
VERSION     DATMFS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198538"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VNQ33_06490"
     CDS             complement(<1..541)
                     /locus_tag="VNQ33_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97790.1"
                     /translation="MGTAVFWGGLGASALLLGAVAAYALRPGPRLVALVMALGSGLLI
                     GSVSFELIDDAVQNAEVAQVGLWALAGALVFTAGDWYLGRRGRRSGGSSDASSSLAIV
                     FGAVLDGIPESFVLGLTVREGGVSVALLAAVVLSNLPEGVSSSTGLRASGWSLGRVAA
                     MWSVVVVVSAVASGAGYVVL"
     gene            complement(1057..1842)
                     /locus_tag="VNQ33_06495"
     CDS             complement(1057..1842)
                     /locus_tag="VNQ33_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWJ97791.1"
                     /translation="MVAPIDAELLDFAVDLTRQAGELTLRYFESDDLAVDRKGDGTPV
                     TAADRGAERLLRELIEERFPDDGILGEEEAEKPGTSGRRWIIDPIDGTKAFTHGVPLY
                     SNLLAVEDADGIAVGVINIPALGETVAAGRGLGCFHNGARCWVDDHATIEGAYLSSSR
                     FTSWDEDCLLRAQRAGLDLRTWGDGYGYVLVATGRIQAMFDPQAELYDLAPMPVILAE
                     AGGRFTSVDGLDGPGHGSGLATNGKVHDELLTILRRPSLTSEN"
     gene            complement(2292..3038)
                     /locus_tag="VNQ33_06500"
     CDS             complement(2292..3038)
                     /locus_tag="VNQ33_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ97792.1"
                     /translation="MRVSPVYDRIGDGYSAQRTPEPTWAAQITAALGGARSILNVGAG
                     SGNYEPLDRPVVAVEPSSTMLAQRANPHPAVRGVAEHLPFRSGVFDAALAVFTVHHWS
                     DRAAGLSELRRVSDRQVLVVYEPLVAHRFWLVDYFPEVAAFTVEADAPTPDEISRYLD
                     VVDVQTLWIPAECCDGVAAAYWRRPEAYLNPVVQQSMSVLALLPADAVARGAARLASD
                     LEDGSWAARHGGLLEQAEADYGYRLVLASS"
     gene            3658..4374
                     /locus_tag="VNQ33_06505"
     CDS             3658..4374
                     /locus_tag="VNQ33_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWJ97793.1"
                     /translation="MPAEIVDALSDGETSTVNFMEQFALDLDRLVECRLPEVRPRQRL
                     MDLSLKARLTQAGRLLAEVSPQQRLYERVANEPDTVRAVAAFAAVHAEDSGASAALVS
                     LRPFADDPHFSVREWAWIALRDRHGDAVVDLVPDLARWAADPSPNIRRFGSEVLRSRG
                     VWSRHLGPVRADPEIASPVIGELRADPHRYVQLSVGNWLNDAGKDHPRWVSDLCASWL
                     SNQPAASTKFICRRGLRNLR"
     gene            complement(4367..4981)
                     /locus_tag="VNQ33_06510"
     CDS             complement(4367..4981)
                     /locus_tag="VNQ33_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97794.1"
                     /translation="MRRRPALDHAPLGPASRSTGPTPQLEYFPGEVAQLLDLGDITYE
                     QLRTLLLIARAARGEALEPGAWARFSVEDLASTEVLVGLVGGRERFREKRRLTISDIE
                     PACRELRKIGFANPLLEVPLARHGRLIVALVGRHVYSPTTGQFAFAFMGEQVEQFVAR
                     RAAHDREARTLLRQVQRERRERRAAARTPMAELEARVAALSSAI"
     gene            5031..5801
                     /locus_tag="VNQ33_06515"
     CDS             5031..5801
                     /locus_tag="VNQ33_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HWJ97795.1"
                     /translation="MTLLDPAPARERPTHEARLSVVLCGSFRRDAEGLQRSLRLLRHD
                     FDVLSPMSLDFVDPAAEFVRLEHEIGEPESTVERRHLDAIGRADFVWLHAPEGYVGTS
                     AAMELGHASAQGVAVYTDTAPSDPVLASLVSVVPSPEFIPRVADRAGGDPGTGVGRLQ
                     RYYATVAARRGWANESPRDTLLLMTEEMGELARAVRMAEGLDRQHSDSGSNLAEELAD
                     VQLYLVHLANALGLDLAAAVSQKEARNSARFEQSLHVA"
     gene            5791..7989
                     /locus_tag="VNQ33_06520"
     CDS             5791..7989
                     /locus_tag="VNQ33_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97796.1"
                     /translation="MSPEPGLTFDQLAEIRRELVQDVRPHRDSINFFATTIGFARTAD
                     QVVAQPASTEPPAIEPLANGTAPAGITTALTCFESLHSAILNEGKSDPAAFAGLTSSD
                     TARVRQFCENALAHPGEWKSENSARRYCIVRALPPVLQLFHAADRSKETALLREVWDL
                     VDLKPESCGVFELAPQIPEHNEPATPYPPNAFLTYWALKAARLLGLSEPDDIRTRSSI
                     NLWLRGVIGRELALHSQDNRRADPQQLAWAIASLVAESESALADHPDATSALIEAGLD
                     SFFAQQSASGTWDTGRPLFHYLRAGNAYCYIYETLAELIDLGLQPEHSASVEVRRLLV
                     RHLPELLRAREHLLSTARPLGDERNGLLGWSSGHHPHRTAPESWATATAFRFLQGLRR
                     LVGLEVRDQAAATLQARRARHDEETLIDRGKTWDSGRGQIGTILLNGLVRPVTAANAR
                     ADGYLDPDRPRIPKSVPRSAILYGPPGTGKTTLAESVAASIGWRFVEVTSADFLSEGS
                     DQVSARADEIFRQLMELDEVVVLFDEVDELIRSRSRPGEMAGRFFTTTMLPRLARLWE
                     RRRTVFFLNTNGIRNVDPAVQRSQRFDAALLVLPPSFDKKLEMLHESVRDYFDLERIT
                     KLLDDYVDKSDDFSASEAQEAWLTFLTFDQLKLIKGGEGMSKEDLYAKLTEFGRELPP
                     DWELASEEKSGDALKTDVLKLQYVIKAYNEERAHQRLDSTFLAQLDAAQG"
     gene            7999..8550
                     /locus_tag="VNQ33_06525"
     CDS             7999..8550
                     /locus_tag="VNQ33_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97797.1"
                     /translation="MATDEISVILVGGEIAAGKTTLATAVASASGARVVRVREAMQEI
                     LGGIGWDRRRLQAEGVSLDRRTNGRWLLDYLEELSESEKRLIVDAARTRRQVEPILES
                     LSGARLVYLTASESVRRDRFTEAQNTDPVKYSMPFDEAMAHVTEREVRDLIAMAHLAH
                     DTDDQTADEVSDAILTWSGWLHP"
BASE COUNT         1620 a         3255 c         3025 g         1461 t
ORIGIN      
        1 cgagcaccac gtagccggcg ccggaggcga ccgccgacac gacgacgacc accgaccaca
       61 tcgccgcgac ccggccgagc gaccagcccg aggcacgaag gccggtcgac gacgacacgc
      121 cctcggggag gttgctcagc accacggcgg ccagcagcgc cacgctcacc ccgccctcgc
      181 ggacggtgag cccgagcacg aacgactcgg ggatgccgtc gagcacggcg ccgaacacga
      241 tcgccagcga gctcgacgca tccgacgatc cacccgagcg ccgtccgcgc cgcccgaggt
      301 accagtcacc cgccgtgaag acgagggcac ccgcaagggc ccagagcccc acctgggcga
      361 cctcggcgtt ctgcacggca tcgtcgatga gctcgaacga caccgacccg atgagcagtc
      421 cgctgccgag cgccatgacg agcgcgacca accgaggacc cggccgcagc gcgtacgccg
      481 cgacggcgcc gagcagcagc gccgacgccc ccaacccgcc ccagaagacc gcagtgccca
      541 cacccggacg ctacgaccgc agcgaatcgc ccgcggcggt ccccgccacc cgccgtcgcc
      601 ctggccgcgg ggcgggcggg cgggcgggcc cacgggatcc ccacgatctt cccgtacggc
      661 aagctgcgcc tcgacgagca cggcgtcccc tctgggtcgg cctgccctcg cccggccggg
      721 tcgacccgtg atgggtccgg ccgcgtgagg cgggtcagcc ggtgaaccag ggcatcgtcg
      781 accggctcgc accaaccccc ccgaacgggc tggcctccga gatcctggcg gagcacgcac
      841 atcgaacgcg ctttgacggg gagctacagc ggaccgaagc ccaacgagca gtcagacatc
      901 gctcattctg ggagcgaccg cacccgcagt cccgctgccg aggcgctcct cggtcagctg
      961 tcggcccacc cagcgtccca ggtcgctcag gcagccaggc gcgccatggc ggcgtggacg
     1021 ctcgcggatg aggcctagct gctagatgat caagagtcag ttctcggacg taagggaggg
     1081 tcgtcggagg atggtgagca gctcgtcgtg caccttgccg ttcgtggcga gaccgctgcc
     1141 gtgacccggt ccgtcgaggc cgtcgacgct tgtgaaccga ccgccggcct cagcaaggat
     1201 gaccggcatc ggggccaggt cgtagagctc ggcctgcgga tcgaacatgg cctggatgcg
     1261 accggtcgct acgagcacgt agccgtaacc gtcgccccag gtccgtaggt cgaggccagc
     1321 ccgctgggcg cggagcaggc agtcctcatc ccaggatgtg aagcggcttg agctgaggta
     1381 cgcgccctcg atggtggcat ggtcgtcgac ccagcagcgg gcgccgttgt ggaagcaccc
     1441 cagaccgcgg ccggcagcaa cggtttcgcc caaggccggg atgttgatta cgcctaccgc
     1501 aatgccatcg gcgtcttcga ccgccagcag gttgctgtac agcgggacgc cgtgggtgaa
     1561 ggccttggtg ccgtcgattg ggtcgatgat ccaacggcgt ccagacgtac cgggcttctc
     1621 ggcctcctcc tcgccgagga tcccgtcgtc ggggaagcgc tcctcaatga gctcacgcag
     1681 caggcgctcg gcaccgcggt cggcggcggt taccggcgtg ccgtcgccct tacggtcgac
     1741 ggcaaggtcg tcgctctcga aataccgaag ggtcagctct cccgcctgcc gggtgaggtc
     1801 gacggcgaag tcaagcagtt cggcgtcgat cggggcgacc atacgcacag cctcccagac
     1861 tcggcaccag tcgggcgaca acgcaggagg aacaccggcg cgaaccaatt gccacggccc
     1921 aatcgcggtt gtggttgccc tcactacccg acgggctcga accatcgacg ctcaatcgaa
     1981 gtcacaaacg tcctcagggg acagaacagc aaccacaccc ggagacaggc gcgggaggtc
     2041 ctctggcagg tcgacgatgt cgcagacggt ttcgatgtcg ggcacacagc tgctccaagt
     2101 gaacgcgcct tgcggcgtgg aagggatctc ctccgagggt ctctatgcac caagaactgg
     2161 ctcattggct caacgggagg cgatgagctc cgcctcgccg ctccccgctt cgcctcagca
     2221 ctgcccgttt cggggcgctc gagctgtttt gtgccgccag ccaagggcgc tcggcaggag
     2281 caatggccag gtcagctgct ggcgaggacc agccggtagc cgtagtcggc ctcggcctgc
     2341 tcgagcaggc cgccgtgtcg ggcggcccag ctcccatcct cgaggtcgga ggcgagtctg
     2401 gcggctcctc tggcgacggc atcggcgggg aggagcgcga ggaccgacat cgactgctgg
     2461 actaccgggt tgaggtatgc ctccgggcgg cgccaatagg cggcggcgac tccgtcgcag
     2521 cactccgccg ggatccacag ggtctggacg tcgacgacgt cgaggtacct gctgatctcg
     2581 tctggcgtcg gtgcatcggc ttcgacggtg aaggcggcga cctctgggaa gtaatccacg
     2641 agccagaacc ggtgtgcgac gaggggctcg tagaccacga gcacctggcg atcggacact
     2701 cgccgcagct cgctgagccc ggccgcccgg tcactccagt ggtgcaccgt gaacacagcc
     2761 agcgccgcgt cgaacacacc gcttcggaac gggaggtgct cggcgacgcc ccggaccgct
     2821 gggtgcgggt tggctcgctg ggcgagcatc gtcgacgacg gctcgactgc gactaccggg
     2881 cggtcgagcg gctcgtagtt cccggaaccg gccccgacgt taaggatcga acgggcaccg
     2941 ccgagcgctg cggtgatctg cgccgcccag gtcggctcgg gagtccgctg ggcggagtag
     3001 ccgtctccga tccggtcgta gacgggcgat acgcgcatca cctgaagata ggcgccagcc
     3061 aaagagccct tggggccgcg aacggccccg gcagcttggt gcagctcacc acacctcggg
     3121 atgaagcacc cggtgccggc cgatccccgt cgttgaagca ctggcaccgc cctcgacgca
     3181 tcgcgacctc agcccaggag cgaccaccga agcctcgccg caatgctcac cgagcgcgag
     3241 tggctcgaag agctcgccac gctgatggag cagtcgttgc ccctaggacc ccaccgaccg
     3301 cccagaggtc aggtgtccgg ctggtaggcc gatgagccgc tgtctccttg caggtcgaga
     3361 atggctgcgc cgatccgcat ggttgccgca acggcactcc tcacatgctc cggatcggcg
     3421 gatcgggtag cccgggcgag ctctcccacc gccgtcgaca tctggcctac gagatcaacc
     3481 gcgtcagatc cttcgatggg aaggctccgg acgtcgatcc tctgattcac tgaacgggat
     3541 ggtacgcgct gaggagtcac gtcgccttga aggcagtagg ccggtctcgg tcgcaccaga
     3601 gacccagtga gcgacccccc taccgagacg cgaaggggcg cccggacgcc gtcgctcgtg
     3661 cccgccgaga tcgtggacgc cctgtccgac ggtgagacat ccaccgtgaa tttcatggag
     3721 cagttcgctc tcgacctcga tcggttggtc gagtgccgcc tgccagaggt ccggccgagg
     3781 cagcggctca tggacctgtc tctcaaggcg cgcctcacac aggccgggcg cctgctcgcc
     3841 gaggtgtcac cccagcagcg cctctatgag cgcgtagcca atgagccgga cacggtcagg
     3901 gcagtagcag cgttcgcagc ggtccacgct gaagacagcg gagcttcagc agcgctcgtg
     3961 tcgttgaggc ccttcgccga cgatccgcac ttcagcgtcc gggagtgggc gtggatcgcg
     4021 ctgcgagacc gacacggcga cgcagtcgtc gacctcgtcc cggatcttgc gcgttgggca
     4081 gccgatccat cgcccaacat acggcggttc ggcagcgagg ttctgcgtag ccgcggggtg
     4141 tggtcacgcc atctgggtcc tgtgcgcgcc gatccggaga tcgcgtcgcc tgtgatcggt
     4201 gagctccgag cggatccgca ccgctacgtt cagctgtcgg tcggcaactg gctgaacgat
     4261 gccggcaaag accatccgag atgggtctcc gacctgtgcg cgtcctggct ctcgaaccag
     4321 ccagctgcct cgactaagtt catctgccgc cgagggctcc ggaacctcag atagcgctgg
     4381 agagggcggc gactcgcgct tcgagctcgg ccatcggggt cctcgcggct gctcggcgct
     4441 ccctccgctc ccgctggacc tggcgcagca gcgtacgggc ctcgcggtcg tgtgctgccc
     4501 gacgggctac gaactgctcc acctgctcac ccatgaacgc gaacgcgaac tgcccggtag
     4561 tcggggagta cacgtgccgc ccgaccaggg ccacgataag gcgcccgtgc cgggcgagcg
     4621 gcacttcgag aagggggttg gcgaacccga tcttccgcag ctccctgcac gccggctcga
     4681 tgtcgctgat tgtcaggcgc cgcttctccc ggaaccgctc gcggccgccc accagcccga
     4741 cgaggacctc ggtagacgcc aagtcctcga cgctgaagcg cgcccacgcc cccggttcga
     4801 gggcctcgcc gcgagcagcc cgggcgatca acagcagcgt ccgcagctgt tcgtaggtga
     4861 tgtcgccgag atcgaggagc tgcgccactt caccagggaa gtactccagc tgcggcgtcg
     4921 gtccagtaga gcgggaagcc gggccgaggg gcgcgtggtc caacgctggt cggcggcgca
     4981 tagattcctg attgtatgat aagataaata ggaattcaat ggaggctccg atgacgcttc
     5041 tcgacccagc cccagcacgc gaacggccga ctcacgaagc ccgcctctcg gtggtcctct
     5101 gcggctcgtt ccggcgggac gccgagggcc tgcagcgctc cctccgcctc ctacggcacg
     5161 acttcgatgt cctctcgccg atgtcgctcg acttcgtcga cccggctgcc gagttcgtcc
     5221 gcttggagca cgagatcggc gaaccggagt ccaccgtcga gcgccgccac ctcgacgcca
     5281 tcggccgcgc cgacttcgtg tggctccacg cgcctgaggg ctacgtcggg accagcgcag
     5341 ccatggagct cggccacgcc tcggcgcagg gcgtagcggt gtacacggac acggccccgt
     5401 cggacccggt gctcgcaagc ctggtgtcgg tcgtgccgtc acccgagttc atcccgcgcg
     5461 tcgccgaccg ggctggtggc gacccaggta ccggtgtcgg gcggctccag cggtactacg
     5521 ccaccgtggc cgcgcgccgc ggctgggcga acgaatcgcc ccgggacacc ttgctgctga
     5581 tgaccgagga gatgggcgaa ctcgccagag ccgtgcgcat ggctgaaggc ctcgaccgcc
     5641 agcactccga ctccgggtcg aacctcgccg aggagctcgc agacgtgcag ctctacctcg
     5701 tccacctcgc gaacgccctc ggtctcgacc tcgccgcggc ggtgagccag aaggaagcac
     5761 gcaactcggc acggttcgag cagtcgctgc atgtcgcctg agcccggcct cacgttcgac
     5821 cagctcgccg agataaggcg ggaactcgtt caggatgtcc gcccgcaccg cgacagcatc
     5881 aacttcttcg ctacaactat cgggttcgct cggaccgctg atcaagtagt agcgcagcca
     5941 gcctccactg agccgcccgc tatcgaaccg ctggcgaacg gcacagcccc ggcggggatc
     6001 acgactgcgc tcacgtgctt cgaatccctc cactcagcga tcctgaacga aggaaagtcg
     6061 gatccagccg cgttcgccgg actcacctct agcgacaccg cccgcgttcg ccagttctgc
     6121 gagaacgcgc tcgcccaccc aggcgagtgg aagagtgaga actcggcaag acggtactgc
     6181 atcgttcgag cgctccctcc agtcctccag ctcttccacg ctgcagaccg ctccaaggaa
     6241 accgcgctgc tccgcgaggt ttgggatctg gtcgacctca aacctgagtc ctgcggagtc
     6301 ttcgagctcg cccctcagat ccccgagcac aacgagccag caacgccgta cccgccgaat
     6361 gccttcttga cctactgggc actcaaggcc gcccggctgc ttggactctc cgaaccggac
     6421 gacatccgga cgcgctcatc gatcaacctc tggctccgag gagtaatcgg ccgcgagctc
     6481 gccctgcata gccaggacaa caggcgggct gaccctcagc agctggcatg ggcaatcgca
     6541 tcgctcgtcg ctgaatcgga gtcggcgctc gccgatcatc ccgatgcgac ctccgccctc
     6601 attgaggccg gcctcgactc cttcttcgct cagcagagcg cgtcgggtac atgggacacc
     6661 ggcaggccgc tgttccacta cctacgcgcc gggaatgctt actgctacat ctatgagact
     6721 ctcgctgagc tgatcgatct cggcctccag ccggagcact cggcttcggt tgaagtccgg
     6781 cgcctcctgg tgcgccatct tcctgagctt ctgcgggcgc gcgagcatct tctgtcgacg
     6841 gcccgtccgc tcggcgacga acggaacggc ctcctcggct ggtcgtcagg ccaccacccc
     6901 caccggaccg cacccgagag ctgggcgaca gcaaccgcat tcaggttcct ccaggggctc
     6961 cgccgcctgg tcggcctcga ggtgagagac caggctgccg ccacccttca ggcgcgccgg
     7021 gctcggcacg atgaggagac ccttatcgat cggggaaaga cctgggacag tggtcgcggc
     7081 cagatcggca cgatccttct gaacggcctc gtccggccgg tcaccgcggc gaacgcccgc
     7141 gccgatggct acctcgaccc cgatcgaccg aggattccaa aaagcgttcc gcgctcagcc
     7201 atcctctacg ggcctcccgg gactggcaag accacactgg cggagtcagt cgctgcctcg
     7261 atcggctggc gcttcgtcga ggtgacctcc gcagacttcc tgtccgaggg ctccgatcag
     7321 gtgtccgctc gcgccgacga gatcttccgc cagctcatgg aactcgacga agtcgtcgtt
     7381 ctattcgacg aggtcgacga gctgatccgc agccgcagca gaccagggga gatggctggc
     7441 cggttcttca ccaccacgat gctcccccgg ctggctcgtc tttgggaacg gcgcaggacg
     7501 gtgttcttcc tcaacaccaa cgggatccga aacgtggacc ctgcggtaca gcgcagccag
     7561 cggttcgacg ctgcactcct cgtcctgcca ccgagcttcg acaagaagct cgagatgctg
     7621 cacgagtccg tgagggacta cttcgacctc gagcgcatca ccaagctctt ggacgactac
     7681 gtcgacaagt ccgacgactt ctcggcatcg gaggcccagg aggcctggct caccttcctc
     7741 acctttgacc agctcaagct gatcaaaggc ggtgagggca tgtccaagga agacctgtat
     7801 gcgaagctga ccgagttcgg acgtgaactg ccgcccgact gggagctcgc gagcgaggaa
     7861 aagtcgggtg acgccctgaa gacagacgtc ctcaagctcc agtacgtgat caaggcctac
     7921 aacgaggaac gagcccacca gcggctcgac tcgacgttcc tcgcccagct tgacgctgcc
     7981 caggggtgac ggtgcgtcat ggccaccgac gagatcagcg tcatcctcgt aggtggtgag
     8041 atcgccgctg gtaagacaac cttggccact gccgtcgctt ccgcttcggg tgcgcgagtt
     8101 gtgcgtgtga gagaggcgat gcaggagatc ctcggcggca tcggctggga ccggcggaga
     8161 ctgcaggcgg agggcgtgag cctcgaccgc cggaccaacg gccggtggct gctcgactac
     8221 ctcgaggagc tgtccgagtc tgagaagcgg ttgatcgtcg acgccgcccg gacacggcgg
     8281 caggtggagc cgatcctcga gtccctcagt ggagcacgtc tcgtctacct cacggcctca
     8341 gagtcggttc ggcgcgaccg gttcaccgaa gctcagaaca ccgacccggt gaagtactcg
     8401 atgccattcg acgaggcaat ggcccacgta accgagcggg aggtccggga cctgatcgcg
     8461 atggcacacc tcgcccatga cactgatgat cagacggccg acgaagtctc tgatgcgatc
     8521 ctcacgtggt ccggatggct gcatccttag agcccacact gaagtccagg ttcgaccacc
     8581 gatccggtcg ggttcatcgg ggcgggtcca tgtagcggcg gacggtgcac gctctcgctg
     8641 tcccgacagc cgcccgcgag cgggctacta gttcactggc actggggcct tgcccgtggg
     8701 tagaccgact cgcaacagcc gctcctagta tcggtcccat ctcggccgcg ggagcaaccc
     8761 caccacgtcc taaggagtct gggtgccgca ggttcgcccg atgtggacct tggaccctga
     8821 tcgcaccaaa tagggtcggc ccgtgctatt ccgacgcggc ttagcaatct tgctcgtctt
     8881 ggcaggaact gtgggggcgc taggagtcgg acttgcgctc ctgggccggt cccaaccacc
     8941 ttcggtcgaa gggatggcag gaggcgaccg gctggccgtg atcgcgaccg agtcgtccgt
     9001 tcaggtaacc gtccaggtca cggcgacaag gagtgggctg gatctgacac tcacgtcgtc
     9061 tgtacggcca tccggcccgt acgtactgct gctgaacctg aacgataacc gggagcgtcg
     9121 gcttgaagcc cttgtcggct ccctcgagcg caaaccgctg ccctcagggc catacgccgt
     9181 ctacgccaac ggagcactag aggttggctc gatgactgtc atcttgctca cgccatcggc
     9241 cacaaccgtg gatttcgggc tccttcggcc aacgcgccag tacaaggcgc aggttcgcat
     9301 tggcccaggc gaccgttatt tcaggtctag aggggcgacc caggaggtac gcataccttc
     9361 a
//
LOCUS       DATMFS010000356         4429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_221504, whole genome shotgun sequence.
ACCESSION   DATMFS010000356 DATMFS010000000
VERSION     DATMFS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221504"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VNQ33_06530"
     CDS             <1..285
                     /locus_tag="VNQ33_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWJ97798.1"
                     /translation="ALAVGLDPDEVDAVLAGDAFAAEVRQDIDAARQIGIAGVPFFVI
                     DGRLGISGAQPPETLKDLLDQGWLTREPAPVQVVAGSEDADACGPDGCEI"
     gene            324..1025
                     /locus_tag="VNQ33_06535"
     CDS             324..1025
                     /locus_tag="VNQ33_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HWJ97799.1"
                     /translation="MSRLFAIPPWTEVRRRLVPLIVGLVLCGVAFGCIVKADLGLDPW
                     DVLHQGISDRTGIPIGTVSVLVSLVVILGWIPLRQRLGLGTVLNALLIGMTMDVVIPR
                     LPHPGSAVQQWGLLVAGLAIAGPGIGMYIGARLGPGPRDGIMTGLAERGPSIRLVRTG
                     IELTALAAGWLLGGDVGIGTVLFALTVGPNAQFWLGRLDLGFPPGFEPVAHADGHHAD
                     DPPPDVEPDFTISGT"
     gene            complement(1324..2199)
                     /locus_tag="VNQ33_06540"
     CDS             complement(1324..2199)
                     /locus_tag="VNQ33_06540"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ97800.1"
                     /translation="MEELSGKVAVVTGAASGIGLAMVQAFVAEGMQVVMADIEAEALE
                     KAAAELPEGTEVLTVVCDVADPAQVDGLRDAAVERFGTAHVVCNNAGVSAGGPIWLAG
                     DDEWDWVLGVNLRGVISGVRAFTPLLIDQGEGHIVNTASMAGLVSAPLMGVYNVSKHG
                     VVTLSETLAAELEMVGASGVGVSVLCPGWVRTRIHEAGRNRPASAEPTSVETMDESMR
                     ELIAGVIAAGLDPADVAAMVVHAIKTSSFYVLTHPDWAHFVSERTDRIVAGQNPIVAS
                     LPSGPGDDDAPALGS"
     gene            complement(2276..3745)
                     /locus_tag="VNQ33_06545"
     CDS             complement(2276..3745)
                     /locus_tag="VNQ33_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWJ97801.1"
                     /translation="MTAIEVDRLRRTLPDDDDHPYRSGAWRPQATEWRVDDVEVVEGA
                     IPDDLDGLYVRNTENPLHEPISRYHPFDGDGMVHAIRFAGGKARYRNRFVRTEGLLAE
                     QEAGASLWAGLAEDPRRAIRTDGLTARGAMKDASSTDLVVHDGEVLTSFWQCGELYRM
                     DPDTLDTLGPTRWDGWFPAEGVSAHTKVDEATGELFFFNYGTEWPYLHFGCVDRTGRL
                     AHYTPIPLPGPRLPHDMAISEHFVVLNDLPMHWDEDLLLNGVHAVRMHDAPTRLAVVP
                     RRGTEEDVQWFEADPTYVLHWTNAYEDGEDVVLEGFHQGCPSPRKPEIGDDMWMYRYL
                     ALDWMETRLHRWRLNRRTGVCTEERMRDGFSEFGIINGRHVGREHRYVWSATGKPGWF
                     LFDGFVRHDVQTGDDDVLRLPDGVYGSEVGVAPKAHAAAEDDAYIVTFTTDPGNERSE
                     CLVLDGRRISDGPIARLRLPEQISSGTHAAWAPGSALPS"
     gene            3846..4223
                     /locus_tag="VNQ33_06550"
     CDS             3846..4223
                     /locus_tag="VNQ33_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97802.1"
                     /translation="MLRPILAATAVLAVVTVAVVGITGGYGADADEAALAIRGTRTVG
                     FSADLQLDVECADHVRVEIRDDPEGSGLDQVTVWGRPRVGRCTPADAMVNTLELDRRA
                     ADRGQEPPTKFVDGATSQVVEIA"
     gene            complement(4230..>4429)
                     /locus_tag="VNQ33_06555"
     CDS             complement(4230..>4429)
                     /locus_tag="VNQ33_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HWJ97803.1"
                     /translation="HGGGGLAVASNHPGGDPEPTEVDRVLASAVSVGAASVGLRFVGH
                     VAVAGRLWEEVPVPHRPPVPG"
BASE COUNT          584 a         1662 c         1549 g          634 t
ORIGIN      
        1 gccctcgccg tcgggctcga ccccgatgag gtcgacgcgg tgctggccgg cgatgcgttc
       61 gccgccgagg tgcgccaaga catcgacgcc gcccgccaga tcggcatcgc cggcgtcccg
      121 ttcttcgtga tcgacggacg gctcggcatc tcgggcgcgc agccgccgga gaccctcaag
      181 gacctcctcg atcaggggtg gctcacgcgc gagccggcac cggtgcaggt cgtggccggc
      241 agcgaggacg ccgacgcctg cggccccgat ggctgcgaga tctgacgtgg gccgggctcg
      301 gcaccaccgg tagcgtccga cctgtgtcgc gcctcttcgc catcccgccg tggaccgagg
      361 tccgccggcg cctcgtcccc ctgatcgtgg ggctcgtgct gtgcggcgtg gcgttcgggt
      421 gcatcgtcaa ggccgacttg ggcctggacc cctgggacgt gctccaccag ggcatctccg
      481 accgcaccgg catcccgatc ggcaccgtca gcgtgctcgt ctccctcgtc gtcatcctcg
      541 gatggatccc gctgcgccag cggctcggac tcggcaccgt cctcaacgcc ctgctcatcg
      601 ggatgacgat ggacgtcgtc atcccgcggc tcccccaccc gggttccgcc gtccagcagt
      661 ggggcctgct cgtcgccggc ctcgccatcg ccggccccgg catcggcatg tacatcggcg
      721 cccgcctcgg acccggtcct cgcgacggga tcatgaccgg gctggccgag cgcggcccgt
      781 cgatccggtt ggtgcggacc ggcatcgagc tcaccgcgct cgccgccggt tggctcctcg
      841 gcggcgatgt cggcatcggg acggtgctct tcgccctcac ggtcggcccc aacgcgcagt
      901 tctggctggg gcggctcgac ctcggcttcc cgcccggctt cgaacccgtg gcccacgccg
      961 acggccacca cgccgacgac cccccgcccg acgtcgagcc cgacttcacg atctccggca
     1021 cctgatcctc cgggttcccg cagaacttgc ggtgaagtcg ctgaccatcg ctcatgagcg
     1081 gcgctccatg gccgatgatc gaagcaacga cgcgcgccgg aggggcgtgc ccaggaggga
     1141 aagccgatgg agcaggcgcg cctgcaggcg atcgacgcca cggcaggagc cgcggcgtgg
     1201 accggcccca ccagcgatgc cgtcccggca cgcgtcgtcg accacctgcg ctctccctga
     1261 tcaaccccgt cccttcggac gcaccgtcgc ccccaagcga accggcgccc ccgcactcgt
     1321 ctcctaggag cccagggcgg gggcgtcgtc gtctccaggc ccgctcggca gcgaggcgac
     1381 gatcgggttc tgaccggcca cgatccggtc ggttcgctcc gacacgaagt gcgcccagtc
     1441 cggatgggtc aggacgtaga agctgctggt cttgatggcg tggacgacca tggcggcgac
     1501 gtccgcgggg tccagcccgg cggcgatgac gccggcgatg agctcgcgca tgctctcgtc
     1561 catcgtctcg acgctggtcg gctcggccga ggcgggccgg ttgcgcccgg cctcgtggat
     1621 gcgggtccgc acccagccgg ggcacaggac cgagaccccg acaccggacg cgcccaccat
     1681 ctccagttcg gccgccaggg tctccgacaa ggtgacgacg ccgtgcttcg acacgttgta
     1741 gacgcccatc agcggcgccg agaccagccc ggccatcgac gccgtgttca cgatgtggcc
     1801 ctcgccctgg tcgatgagca gcggggtgaa cgcccgcacg ccgctgatca cgccgcggag
     1861 gttgacgccc agcacccagt cccactcgtc gtcgccggcc agccagatcg gtcccccggc
     1921 cgagacgccg gcgttgttgc acacgacgtg ggcggtgccg aagcgctcga ccgcggcgtc
     1981 gcggagaccg tcgacttggg ccgggtccgc cacgtcgcac accacggtca gcacctcggt
     2041 gccctcgggc agctcggcgg ccgccttctc cagggcctcc gcctcgatgt cggccatcac
     2101 gacctgcatg ccctcggcga cgaaggcctg gaccatcgcc aggccgatgc ccgaggccgc
     2161 accggtcacg accgcgacct tgccggaaag ctcttccatg accactcccc tgtggtgctg
     2221 gggccgcggg gggatgcggc cggtggcggc accctaccca accttgaccc gcgggtcagg
     2281 agggcagggc cgaccccggg gcccaggcgg cgtgggtgcc gctggagatc tgctcgggca
     2341 ggcggagccg ggcgatgggc ccgtcgctga tgcgccgacc gtcgaggacg aggcactcgg
     2401 atcgctcgtt gcccgggtcg gtcgtgaacg tgacgatgta ggcgtcgtcc tcggcggcgg
     2461 cgtgggcctt cggggccacg cccacctcgg atccgtacac gccgtccggg aggcgcagga
     2521 cgtcgtcgtc gccggtctgg acgtcgtggc gcacgaaccc gtcgaacagg aaccagcccg
     2581 gcttgcccgt ggcggaccac acgtagcggt gctcccggcc cacgtggcgg ccgttgatga
     2641 tcccgaactc ggagaagccg tcgcgcatcc gctcctcggt gcacacgccc gtccgccggt
     2701 tgagccgcca gcggtgcagc cgcgtctcca tccagtcgag ggcgaggtag cggtacatcc
     2761 acatgtcgtc gccgatctcc ggcttgcggg gcgacgggca gccctggtgg aagccttcga
     2821 gcacgacgtc ctcgccatcc tcgtaggcgt tggtccaatg gaggacgtag gtggggtccg
     2881 cctcgaacca ctgcacgtcc tcctcggtcc cccgccgggg cacgacggcc aggcgggtgg
     2941 gcgcgtcgtg catgcgcacc gcgtggacgc cgttgaggag caggtcctcg tcccagtgca
     3001 tggggaggtc gttgagcacg acgaagtgct cgctgatggc catgtcgtgg ggcaggcgcg
     3061 ggccggggag cgggatcggc gtgtagtggg cgaggcggcc ggtgcggtcg acgcagccga
     3121 agtggagata gggccactcg gtcccgtagt tgaagaagaa cagctcaccg gtggcctcgt
     3181 cgaccttggt gtgggcgctc acgccctcgg ccgggaacca cccgtcccag cgggtgggcc
     3241 cgagcgtgtc gagcgtgtcc ggatccatcc ggtacagctc gccgcactgc cagaacgagg
     3301 tcaggacctc gccgtcgtgc accaccaggt ccgtgctgct ggcgtctttc atcgcacccc
     3361 gggcggtcag cccgtcggtg cggatggcgc gccgcgggtc ctcggccagt ccggcccaca
     3421 gcgacgcacc cgcctcctgc tcggcgagca gcccctcggt gcggacgaac cggttccggt
     3481 agcgggcctt cccgccggcg aagcggatgg cgtgcaccat cccgtcgccg tcgaacgggt
     3541 ggtaccgcga gatcggctcg tgcagcgggt tctcggtgtt gcggacatag agcccgtcga
     3601 ggtcatcggg gatcgccccc tccaccacct cgacgtcgtc gacccgccac tcggtggcct
     3661 gcggccgcca cgcacccgac cggtacgggt ggtcgtcgtc gtccggcagc gtccgccgca
     3721 gccggtccac ctcgatcgcg gtcacacgac caccccctgg cgtcacgacc gcgaccatgt
     3781 gtcctcggtc acgtccgagc gtaccggcct cggagcgccg cccctcccgc tcctacgctg
     3841 ggccggtgct ccgcccgatc ctcgccgcga ccgccgtgct ggccgtcgtg accgtggccg
     3901 tcgtcgggat caccggcggg tacggcgccg atgcggacga ggcggccctc gccatccgcg
     3961 ggacccgcac cgtgggcttc agcgccgacc tccagctcga cgtcgagtgc gcggaccacg
     4021 tgcgcgtcga gatccgtgac gatcccgagg gcagcgggct cgaccaggtc acggtgtggg
     4081 gacgtccacg ggtcgggcgc tgcaccccgg ccgatgccat ggtcaacacg ttggagctcg
     4141 accgccgggc cgcggatcgc ggccaggagc cgcccacgaa gttcgtcgac ggggccacga
     4201 gccaggtggt cgagatcgcc tgaccgggct cagcccggca ccggcggtcg gtgcgggacc
     4261 gggacctcct cccacaggcg gccggcgacc gcgacgtgcc cgacgaaccg cagcccgacg
     4321 gacgcggcgc cgacgctcac ggccgacgcc agcacccgat cgacctccgt cggctcagga
     4381 tcgccgccgg ggtggttgct cgccacggcc agtcccccgc cgccgtggt
//
LOCUS       DATMFS010000357         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_243644, whole genome shotgun sequence.
ACCESSION   DATMFS010000357 DATMFS010000000
VERSION     DATMFS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243644"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VNQ33_06560"
     CDS             <1..760
                     /locus_tag="VNQ33_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWJ97804.1"
                     /translation="TLVTEIGDKLDVIMIPKVEGAEDIHYVDRLLAQLEARAGITKPI
                     LVHALLETARGVANVEEICAASPRMQGLSLGPADLAADRRMKTTRVGGGHPGYLVRQD
                     PVDGDIEGTTSARTTYQQDLWHYTIARMVDACQANGIFAFYGPFGDIKDVVACEDQFR
                     NAFLLGCVGAWSLHPVQIDIAKRVFSPDPADVAHALHVIEEMGDGTGAVMIDGKMEDD
                     ASVKQCHVMVDLAKQLSARDEDVAALYRAVGLEV"
     gene            765..1655
                     /locus_tag="VNQ33_06565"
     CDS             765..1655
                     /locus_tag="VNQ33_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWJ97805.1"
                     /translation="MATGSSDGVILPRRSVLYMPGANERALEKAKGLDADALILDLED
                     AVAPDAKDEARERVCAAASSGEYGSKEVTIRVNGLATQWHADDIAAAAEARPAAVVVP
                     KVDSVADVHAIEQGLEAGGAPDTTKIWAMVETPVAMLHAEEIAAASERLTVLVMGTND
                     LAKELHAEHVPGRQPLLTGLGLCLLAARATGKVILDGVYNDITDEAGFEAECLQGRQM
                     GFDGKTLIHPSQLEPANRIWAPTAEAVEDAAALIATFEEAVAAGKGVVTHNGRMIENL
                     HVDNARRILAVADAIAARQA"
     gene            1746..>2192
                     /locus_tag="VNQ33_06570"
     CDS             1746..>2192
                     /locus_tag="VNQ33_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97806.1"
                     /translation="MADDTPGRAGSDGGTAPEPGPAPTGPPPGFAGGTPTPPPGFAGS
                     GSGASTPGGPPRGFAGANPDVADPYASTPYAQVPAPPTRSSPPPKKGKGGVIALLVVI
                     VALAAGGIWFAASSGDDDSASSSEVAEGGPGATVEAYWQALKDSDWE"
BASE COUNT          332 a          867 c          724 g          269 t
ORIGIN      
        1 cacgctcgtc accgagatcg gcgacaagct cgacgtgatc atgatcccga aggtcgaggg
       61 cgccgaggac atccactacg tggacaggct cctcgcccag ctcgaggccc gggccggcat
      121 cacgaagccg atcctcgtcc acgccctgct cgagaccgcc cgcggcgtcg ccaacgtcga
      181 ggagatctgc gcggccagcc cccgcatgca ggggctctcg ctcggcccgg ccgacctcgc
      241 cgcggaccgc cgcatgaaga ccacccgcgt cggcggcggc caccccggct acctggtgcg
      301 ccaggacccg gtcgacggcg acatcgaggg caccaccagc gcccgcacca cgtaccagca
      361 ggacctgtgg cactacacga tcgcccgcat ggtcgacgcc tgccaggcca acgggatctt
      421 cgccttctac ggccccttcg gcgacatcaa agacgtcgtg gcctgcgagg accagttccg
      481 caacgccttc ctgctcggct gcgtcggcgc ctggtcgctg cacccggtcc agatcgacat
      541 cgccaagcgc gtcttctcgc ccgacccggc cgatgtcgcc cacgccctcc acgtgatcga
      601 ggagatgggc gacggcaccg gcgccgtcat gatcgacggg aagatggaag acgacgcctc
      661 cgtgaagcag tgccacgtga tggtcgactt ggccaagcag ctctcggccc gcgacgagga
      721 cgtcgccgcg ctctaccgcg ctgtgggcct ggaggtctga tccgatggcc accggttcct
      781 ccgacggcgt gatcctcccc cgccgctccg tcctctacat gcccggcgcc aacgagcggg
      841 cgctggagaa ggccaagggg ctcgatgccg acgcgctgat cctcgacctc gaagacgccg
      901 tcgcccccga cgccaaagac gaggcccgcg agcgcgtgtg cgccgcggcg tcgtccggcg
      961 agtacggctc caaggaggtg accatccgcg tcaacggcct cgccacccag tggcacgcgg
     1021 acgacatcgc ggcggccgcc gaggcccgcc cggccgcggt ggtggtgccc aaggtcgact
     1081 cggtcgccga cgtccacgcc atcgagcagg gcctcgaagc gggcggcgcg cccgacacca
     1141 cgaagatctg ggcgatggtc gagacacccg tcgccatgct ccacgctgag gagatcgcgg
     1201 cggcgtccga gcgcctcacc gtgctggtca tgggcacgaa cgacctggcc aaggagctcc
     1261 acgccgagca cgtgcccggc cgccagccgc tgctcaccgg gctggggctg tgcctcctgg
     1321 ccgcccgggc caccggcaag gtcatcctcg acggcgtcta caacgacatc accgatgagg
     1381 ccggcttcga ggccgagtgc ctccagggcc gccagatggg cttcgacggc aagaccctca
     1441 tccacccgag ccagctcgag ccggccaacc ggatctgggc ccccaccgcc gaagcggtcg
     1501 aggacgccgc cgccctcatc gccaccttcg aggaggcggt cgccgccggc aagggcgtcg
     1561 tgacccacaa cgggcggatg atcgagaacc tccacgtcga caacgcccgg cgcatcctcg
     1621 ccgtcgccga cgccatcgcc gcccgccagg cctgagtccc gggctcaccc gtccccgctg
     1681 catcgaccgc ccgtgcccgg ccgggcaccc gacgacgacg gatctgaggg gttacggtcc
     1741 cgcccatggc tgacgacacc cccggtcggg cgggctccga cggcgggacg gcacccgagc
     1801 ccggcccggc tccgaccggc ccgcctcccg ggttcgctgg cgggaccccc acgcctccac
     1861 ccgggttcgc cgggtccggc tcgggcgcga gcacgcccgg tggtccgccc cgaggcttcg
     1921 ccggcgcgaa ccccgacgtc gccgacccgt acgcgtcgac gccctacgcc caggtgcctg
     1981 cacccccgac ccgctcgtcg cctcctccga agaagggcaa ggggggcgtg atcgccctcc
     2041 tggtcgtgat cgtcgccctc gccgcgggcg gcatctggtt cgccgcctcg tccggcgacg
     2101 acgactccgc ctcgagcagc gaggtggccg aaggcggccc gggtgccacc gtcgaggcct
     2161 actggcaggc gctcaaggac agcgactggg ag
//
LOCUS       DATMFS010000358         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_221529, whole genome shotgun sequence.
ACCESSION   DATMFS010000358 DATMFS010000000
VERSION     DATMFS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221529"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VNQ33_06575"
     CDS             complement(<1..549)
                     /locus_tag="VNQ33_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWJ97807.1"
                     /translation="MTAVPDRPLVPLPGQPDGVPWPTGEWPRAEAADLGADAPRLTAL
                     LDELVSGDDHPALGLTHAAAVVAGGRLVAERYGRRVVQDLRAMGDDPPHEDVDETTGL
                     LSWSMAKTITSLVTGAAVADGALTVDDPVADPRWDAPDDPRKAITWSNLLTMRPGLAW
                     SEEYYDLDPDGLPDVVRMLYGEG"
     gene            complement(569..1582)
                     /locus_tag="VNQ33_06580"
     CDS             complement(569..1582)
                     /locus_tag="VNQ33_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWJ97808.1"
                     /translation="MTDPSTPEPGDPVEVRVAFWNSWLLSPRLWSSGPRMPGLPGWFG
                     PEVEARAPLVARAVAGRFDIVALAECFERSEQDAVARGWPGATFVAGPQRHGVRPQGS
                     GLATLVAPGLRVVRSARHAYRAGGDLRDSDTFATKGAQLVAVQLAEHLPPVEVVSTHL
                     FAGGDLFPIPGAEDAARHHGVRMRQVDELAAFVADQHDPASPLLVVGDFNVRAHDDDP
                     VLAEPQARYRDLAERLASVGLVDVWAGHGRGSGHTCTFRSAADLPADPDEPDQVADDP
                     DAGDDAPGERIDYLWLAAPPGTAVEVERPRRWSFTGRGVQGGPAGSLSDHLALSVTLR
                     LSA"
     gene            complement(1628..>2271)
                     /locus_tag="VNQ33_06585"
     CDS             complement(1628..>2271)
                     /locus_tag="VNQ33_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97809.1"
                     /translation="GTDLIDEPVSIRRGALARLTGPACVPGALTADPAEGEAVLAEAL
                     AAGHEGVVVKASGSPYDAGRRGKAWRKVKPVHTLDLVVLAVEWGHGRRTGWLSNLHLG
                     ARAVDGPAADEPGGFVMVGKTFKGLTDQLLRWQTERFLGLAEHEEPWNGPRTGVVRVR
                     PEQVVEIALDGVQRSTRYPGGVALRFARVVRYRHDKPPAEADPITAVWAMLGS"
BASE COUNT          282 a          827 c          888 g          274 t
ORIGIN      
        1 accctcgccg tagagcatgc ggacgacgtc gggcaggccg tcggggtcga ggtcgtagta
       61 ctcctcgctc cacgcgaggc ccggtcgcat cgtgagcagg ttcgaccacg tgatggcctt
      121 ccgcgggtcg tcgggtgcgt cccaccgcgg gtcggcgacg gggtcgtcga cggtgagcgc
      181 gccgtcggcc accgccgctc cggtcaccag gctcgtgatg gtcttggcca tcgaccagct
      241 gaggagcccg gtcgtctcgt cgacgtcctc gtgcggcgga tcgtcgccca tcgcccgcag
      301 gtcctggacc acccggcgtc cgtaccgctc ggcgaccagc cgcccgccgg ccacgacggc
      361 ggcggcgtgg gtcaacccga gcgccgggtg gtcgtcgccg ctgaccagtt cgtcgagcag
      421 cgcggtgagc cgcggggcgt cggcgcccag gtcggcggcc tcggctcggg gccactcgcc
      481 ggtcggccag gggacgccgt cgggctggcc ggggagcggg acgagcgggc ggtcgggtac
      541 ggcggtcacg gatccgatgg tggcacgctc aggcggacag gcgcagcgtc accgacaagg
      601 cgaggtggtc cgagagcgag ccggcggggc cgccctgcac gccgcggccc gtgaacgacc
      661 accggcgcgg ccgctcgacc tcgacggcgg tcccgggagg cgccgccagc cacaggtagt
      721 cgatgcgctc gccgggagcg tcgtcgccgg catcggggtc gtcggcgacc tggtcgggct
      781 cgtcggggtc agcggggagg tcggctgcgc tgcggaacgt gcacgtgtgg cccgaacccc
      841 tgccgtgacc ggcccacacg tcgaccaggc cgacggaggc gagtcgctcg gccaggtcgc
      901 ggtagcgcgc ctgcggttcg gcgagcacgg ggtcgtcgtc gtgggcccgg acgttgaagt
      961 cgccgacgac gagcaggggg ctcgccgggt cgtgctggtc cgccacgaag gcggcgagct
     1021 cgtcgacctg gcgcatccgg acgccgtggt gccgggcggc gtcttcggca ccggggatcg
     1081 ggaacaggtc cccgccggcg aagaggtgcg tcgacacgac ctcgaccggg ggcaggtgct
     1141 cggcgagctg gacggccacg agctgagcgc ccttggtggc aaaggtgtcc gagtcccgga
     1201 ggtcgccgcc ggcgcggtag gcgtgacggg cggaccgcac cacccggagc cccggcgcca
     1261 cgagggtggc gaggccgctg ccctggggtc ggacgccgtg gcgctggggc ccggccacga
     1321 acgtcgcacc gggccagccc cgtgcgacgg cgtcctgctc ggaccgctcg aagcactcgg
     1381 ccagcgccac gatgtcgaac cggccggcga cggctcgggc cacgagcggc gcccgggcct
     1441 ccacctcggg gccgaaccac ccgggcagcc cgggcatgcg gggaccggag ctccacaggc
     1501 gcggcgagag gagccagctg ttccagaacg cgacccgcac ctcgacggga tcgccgggtt
     1561 cgggggtgct cgggtcggtc acggcgctga ccctaccggc gggtgccgaa cggcttcgac
     1621 gccgccctca ggacccgagc atggcccaga cggcggtgat gggatccgcc tcggcgggcg
     1681 gcttgtcgtg gcggtagcgg accacccggg cgaagcggag ggccacgccg ccggggtagc
     1741 gggtcgagcg ctgcaccccg tcgagcgcga tctcgaccac ctgctcgggc cggacccgca
     1801 ccacccccgt tcgggggccg ttccacggct cctcgtgctc ggcgagaccg aggaagcgct
     1861 cggtctgcca ccgcagcagc tggtcggtga ggcccttgaa cgtcttgccc accatcacga
     1921 atccgccggg ctcgtcggcg gccggtccgt cgaccgcccg tgcaccgagg tggaggttcg
     1981 acagccagcc cgtcctgcgg ccgtggcccc actccacggc gagcacgacc aggtcgagcg
     2041 tgtgcaccgg cttgaccttg cgccacgcct tgccccggcg cccggcgtcg tagggagaac
     2101 ccgacgcctt caccaccacg ccctcgtggc cggcggccag ggcttcggcg aggaccgcct
     2161 cgccctcagc ggggtcggcg gtgagcgcgc cggggacgca cgccggcccg gtgagccgcg
     2221 ccagcgcgcc ccggcggatc gagaccggct cgtcgatcag gtcggtgccg t
//
LOCUS       DATMFS010000359         6796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_58373, whole genome shotgun sequence.
ACCESSION   DATMFS010000359 DATMFS010000000
VERSION     DATMFS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58373"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1676
                     /locus_tag="VNQ33_06590"
     CDS             111..1676
                     /locus_tag="VNQ33_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWJ97810.1"
                     /translation="MPETIAAVDVGTNSLHLVVARTTDGDRFEVLTREKEMVRLGSGS
                     TDMKELSPAAVDRAVDALARFRKIADSHGAPMRAVATSAVREAENRKDLLRRARKEAG
                     VEIEVVSGVEEARLIHLGVLQALELYDRRLILCDIGGGSTELLIGEQGEALASVSVKL
                     GAVRLTNRFFAAGGLHPSAVDSCRRHIQGVLASFDRSVAEHGFEVAVGSSGTIEQVYR
                     LARGLAGEEPLRTLNAAVLTRKQVVSVTEELIRARSAGTTAALPGLDPKRADIILAGA
                     LVLEGVVKRFGIKALVLSEYALREGVFLDTIARRRGGSLPHLRDVALRSAIDLMQACD
                     EDPVHSEHVARLALELFDATADRHGLGADARSLLEAGALLANVGLVISHGKHHKHSYY
                     LIRNSDRLVGFTDQEIEVVAQIARYHRKSAPKASHPEWAALDDDDQRTVVVAAAVLRV
                     AIGLDRSHQQLVTGLDATERDGQLVLTIETRDPEADHSLELYSADERSGLLADVLERP
                     IVVVLDAGAPAGG"
     gene            1725..2744
                     /locus_tag="VNQ33_06595"
     CDS             1725..2744
                     /locus_tag="VNQ33_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97811.1"
                     /translation="MATLVCPECGSQYVASVRRCIDCDAMLVDDVGPAGEAAPSVATP
                     LGEGDQIAYELEGWGNQLRVTLSGMLDLNGIAHVWEVGALVVAAPHEEKVDELIAAVE
                     GGEGEELDADVPQVAFEIEEVSADELAALDAELIAAHIPHAWQETGELLVPEESEEEV
                     AELIEAILNPSEDETEDDGLATHAALDALYVAVDKLGKDPADEKLIARYAAAADGLDG
                     LRVPYGMSGKEWEDLVAQVEVLRTVLAKAGEADGDGDGGDAEAERPEPTVHAVDGQGG
                     GDEGEGGEGEGDDGNAARDANGGLVVGSSDADKADQGDDRPLDGIERARELVGQLRTR
                     MLDLV"
     gene            2773..3471
                     /locus_tag="VNQ33_06600"
     CDS             2773..3471
                     /locus_tag="VNQ33_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWJ97812.1"
                     /translation="MLLAELEVFHSRPIAPTRRVAIGVAVLPTAPPPGFGGLLLGGIV
                     AAFIPEVDPDLLDDLTRLTHQVEAGQRISQPRLRHRLQADRVGLSKCAHRLVGTGEEL
                     RFDLHESGSPEPNVLAAVYAAGRLDPTVRPAVLAAMRRAMHWRADLGPEFVEYLTETK
                     STANWSTLGGRDPVVWALGILGLAPEANERTAVQDRFRDLLRNAHPDHGGDVDDAAER
                     ITELTEARRILLRS"
     gene            3504..4052
                     /locus_tag="VNQ33_06605"
     CDS             3504..4052
                     /locus_tag="VNQ33_06605"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWJ97813.1"
                     /translation="MLLTHGAGGNKDQPALVAIEEALAPTPVRRMNFPYRDLGPKRPP
                     DRAPKLIASVEEQAAALCAEVHVRPPRLALGGRSMGGRMCSMAVADGLPAAALVLIAY
                     PLHPPGKPDKLRIEHLPNISVPCLFVSGTRDPFGSPEELQAATAVIPGPVTHHVVEGK
                     GHDLKGADAAVASVIRDWLRAL"
     gene            complement(4124..5707)
                     /locus_tag="VNQ33_06610"
     CDS             complement(4124..5707)
                     /locus_tag="VNQ33_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ97814.1"
                     /translation="MTPRTTVNELDVDEAGLHAIVVPRDDILLEDLDAADPDGHDFRF
                     VCEQGPFSAYERTVHADRIDAGGYHVREEITWDVAIPLWGALFRPLVARQLRRTGPPE
                     ILDEDEAARRAPWWSPPARLDARSSSVLAMLCGLSMLTGYLGTTITQTMTYAAKEFGA
                     STAAQGATLATVRVGVLLSLVLVTISDRRGRQKLLLVCAVGGVAASVAGAASPDLITL
                     GGTQTVARAFATALALLIGVVSVEEMPAGGRAYAASVLAMTAALGAGMAVILLPVADM
                     GRYAWRIIYVVPLLGLPFFIRLARRIPESRRFVKPHGRATMKGHRRRLALLAAASFAG
                     LVFLAPVSQFQNEFLRTEHGFTAIELTVFTLATNTPGGIGIVVGGRLADIRGRRMVGA
                     IASIGGAALLTVGYQVSGAMLWVAWMAGTVIAAATVPALGVYGAELFPTALRGRANGL
                     ITLAGVAGSSVGLILAGRFETHFGRFGPGMAILAIGPFIVAALVLTLYPETAHVELEV
                     LNPEDALAGGAASVAPPML"
     gene            complement(5704..5871)
                     /locus_tag="VNQ33_06615"
     CDS             complement(5704..5871)
                     /locus_tag="VNQ33_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97815.1"
                     /translation="MLGYAGVLAHQGGWDEALMVIVPVGLFGGLLWVAAKRHAPQDDD
                     PTPPDEGATIP"
     gene            5986..6249
                     /locus_tag="VNQ33_06620"
     CDS             5986..6249
                     /locus_tag="VNQ33_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWJ97816.1"
                     /translation="MEDQVRKTIEVIRPAIQADNGDIFLKGVDEATGVVTVELTGACV
                     SCPASTVTLKAGVERILKDRVPGVTAVEQFGAEPAETESAVSL"
     gene            6271..>6796
                     /locus_tag="VNQ33_06625"
     CDS             6271..>6796
                     /locus_tag="VNQ33_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWJ97817.1"
                     /translation="MTTSDQLDAARRGDRRTLARLLSSIERGDAASHDINRTVFSQVG
                     GAYTVGLTGAPGAGKSTLTSAAVASARRRGERVSVLAIDPSSPFSGGALLGDRTRMND
                     HALDDGVFIRSMATRGHLGGLALATREAIRLLDAVGFPWVLVETVGVGQVEVEVAGAA
                     DTTVVVVNPGWGDAV"
BASE COUNT          958 a         2476 c         2476 g          886 t
ORIGIN      
        1 cgtcgtcaca gggatctgac gtcgccgcgg cgacggtggc gtgcgctgtc tcctcccggt
       61 acgggcgtgg ggacctgatc acctgcggga catcggtagc ctcctcatca gtgccggaga
      121 ccatcgctgc tgtcgacgtg gggaccaact cgctccacct cgtggtggcc cgcaccaccg
      181 acggcgaccg cttcgaggtg ctgacccgcg agaaggagat ggtccgtctc ggctccgggt
      241 ccacggacat gaaggagctg tcgccggccg ccgtcgatcg agccgtcgac gccctcgccc
      301 ggttccgcaa gatcgcggac agccacggag ccccgatgcg ggcggtcgcc accagcgccg
      361 tgcgggaggc ggagaaccgc aaggacctcc tgcggcgcgc ccgcaaggag gccggcgtcg
      421 agatcgaggt cgtgtccggc gtcgaggagg cccgcctcat ccacctcggc gtgctccagg
      481 ccctggagct ctacgaccgc cggctgatcc tgtgcgacat cggcggcggg tccaccgagc
      541 tgctcatcgg cgagcagggc gaggccctgg cgtccgtcag cgtcaagctc ggcgccgtcc
      601 ggctcacgaa ccgcttcttc gccgccgggg ggctgcaccc gtccgccgtc gactcgtgcc
      661 gccgtcacat ccagggcgtg ctcgcgtcgt tcgaccggtc cgtggccgag cacggcttcg
      721 aggtcgccgt ggggtcgtcg gggaccatcg agcaggtgta ccggctggca cgcggcctgg
      781 cgggcgagga gccgctgcgg accctcaacg ccgccgtgct cacccgcaag caggtcgtct
      841 ccgtcaccga ggagctgatc cgggcccggt ccgcgggcac caccgccgcg ctgcccggcc
      901 tggacccgaa gcgggccgac atcatcctcg ccggtgcctt ggtcctcgag ggcgtggtca
      961 agcggttcgg catcaaggcg ctggtgctgt cggagtacgc gctccgcgag ggcgtcttcc
     1021 tcgacacgat cgcccgccgg cggggcggct cgctgcccca cctgcgcgac gtcgccctcc
     1081 gcagcgccat cgatctgatg caggcgtgcg acgaagaccc cgtccactcc gaacacgtcg
     1141 cccggctggc tctggagctg ttcgacgcca ccgccgaccg ccacggcttg ggtgccgatg
     1201 cccggagcct gctggaggcc ggggcgctcc tcgccaacgt ggggctggtc atctcccacg
     1261 gcaagcacca caagcacagc tactacctga tccggaactc cgaccggctg gtggggttca
     1321 ccgaccagga gatcgaggtc gtcgcccaga tcgcccgcta ccaccgcaag agcgcgccga
     1381 aggcgagcca cccggagtgg gcggcgctcg acgacgacga ccagcgcacg gtcgtcgtcg
     1441 ccgccgccgt cctccgggtg gccatcggcc tcgaccgctc ccaccagcag ctcgtcacgg
     1501 ggctcgatgc caccgagcgg gacgggcagc tggtcctcac gatcgagacg cgcgatcccg
     1561 aggcggacca cagcctcgag ctgtactcgg ccgatgagcg cagcggcctg ctggccgatg
     1621 tgctggagcg gcccatcgtg gtggtcctcg acgccggggc gcccgccggg ggttgaggcc
     1681 cagcgcggtc gcggaccacc ggcgacgtct gccaagcttc ggacatggcg acgctcgtgt
     1741 gcccggagtg tggatcccag tacgtggcct cggtgcggcg gtgcatcgac tgcgacgcga
     1801 tgctggtcga cgacgtcggt cccgccggcg aggcggctcc gtcggtcgcc accccgctgg
     1861 gcgagggcga ccagatcgcc tacgagctcg agggctgggg caaccagctg cgggtcaccc
     1921 tgtcggggat gctggacctg aacggcatcg cccacgtgtg ggaggtcggg gccctcgtcg
     1981 tcgccgctcc ccacgaggag aaggtcgacg agctcatcgc cgcggtcgag ggaggcgagg
     2041 gcgaggagct cgacgccgac gtcccccagg tcgccttcga gatcgaggag gtctcggccg
     2101 acgagctggc cgcgctcgat gccgagctca tcgccgccca catcccccac gcctggcagg
     2161 agaccggcga gctgttggtg cccgaggagt ccgaggagga ggtggctgag ctgatcgagg
     2221 ccatcctcaa cccgtccgag gacgaaaccg aagacgacgg cttggccacc cacgccgccc
     2281 tcgacgcgct gtacgtggcc gtcgacaagc tcggcaagga cccggccgac gagaagctga
     2341 tcgcccggta cgcggccgcc gccgacggtc tcgacgggct cagggtgccc tatggcatgt
     2401 cgggcaagga gtgggaggac ctcgtggccc aggtcgaggt gctgcgcacg gtgctcgcca
     2461 aggccggcga ggccgacggt gacggtgacg gtggcgacgc ggaggccgag cgccccgagc
     2521 ccaccgtgca cgccgtcgat ggccaaggcg gcggcgacga gggcgaggga ggcgagggcg
     2581 agggcgacga cggcaacgca gcacgcgacg ccaacggcgg cctggtcgtg ggctcgtcgg
     2641 acgcggacaa agcggatcag ggcgacgacc gccccctcga cgggatcgag cgggcgcggg
     2701 agctggtcgg ccagctgcgg acccgcatgc tcgacctcgt ctgatcgcct ggcgcgccgg
     2761 tagcgtgtcg tgatgctgct ggccgagttg gaggtcttcc actcccggcc catcgcgccc
     2821 acccgccggg tcgccatcgg cgtcgccgtg ctgcccaccg cacctccgcc agggttcggc
     2881 ggcttgctct tgggcggcat cgtcgccgcg ttcatccccg aggtcgaccc ggacctgctc
     2941 gacgacctca cccgcctcac gcaccaggtg gaggccggcc agcggatctc ccagccccgg
     3001 ctgcgccacc ggctccaagc cgaccgggtc gggttgtcca agtgcgccca ccgcctggtc
     3061 ggcacggggg aggagctgcg cttcgacctc cacgagtccg ggtcgccgga gccgaacgtc
     3121 ctcgccgccg tctacgccgc tggccgcctc gatcccaccg tccggcccgc ggtgctcgcc
     3181 gccatgcggc gggccatgca ctggcgcgcc gacctcggtc ccgagttcgt ggagtacctg
     3241 accgagacca agagcacggc caactggtcc acgctcggtg gccgggaccc ggtggtctgg
     3301 gccctcggga tcctcggcct ggcgccagag gccaacgagc gcaccgcggt gcaggaccgc
     3361 ttccgggacc tcctgcgcaa cgcccatcct gaccacggag gcgacgtcga cgacgctgcc
     3421 gagcggatca ccgagctcac cgaggcccgg cgcatcctgc tgcggtcctg acggtggccg
     3481 ggcgcgcccg ccgcgtcggg gccgtgctgc tcacccacgg cgctggcggc aacaaggacc
     3541 agccggcgct ggtggcgatc gaggaggccc tggccccgac gcccgtccgg cgcatgaact
     3601 tcccgtaccg cgacctcggg ccgaagcggc cgccggaccg ggcgcccaag ctgatcgcct
     3661 ccgtcgagga gcaggcggcc gcgctctgcg ccgaggtgca cgtgcggccg ccccgcctgg
     3721 cgctgggcgg ccggtcgatg ggtgggcgca tgtgctcgat ggccgtggcc gacgggctgc
     3781 ccgccgccgc gctggtcctg atcgcctatc cgctgcaccc gccgggcaag cccgacaagc
     3841 tccgcatcga gcacctcccg aacatctccg tgccctgcct gttcgtgtcg ggcacccggg
     3901 accccttcgg gagccccgag gagctgcagg ccgccaccgc ggtgatcccc ggccccgtca
     3961 cccaccacgt cgtcgagggc aagggccacg acctcaaggg cgccgacgcc gccgtcgcct
     4021 ccgtcatccg cgactggctc cgcgccctct gacgaacccg ccccagggcg agcctcgggc
     4081 gcgagggcgt cctcgccggc gaggtccggt gggtcgggcg ggctcagagc atcgggggtg
     4141 cgacgctggc ggcaccaccg gcgagggcgt cctcggggtt caagacctcg agctccacgt
     4201 gggccgtctc cgggtacagc gtcaggacca gggcggcgac gatgaacggg ccgatcgcca
     4261 agatggccat gccgggcccg aagcggccga agtgggtctc gaagcggccg gccaggatca
     4321 ggccgacgct gctcccggcc acgccggcga gcgtgatgag cccgttggcc cggccgcgca
     4381 gggccgtggg gaacagctcg gcgccgtaca cgccgagggc ggggacggtg gccgccgcga
     4441 tgaccgtgcc ggccatccac gcgacccaca gcatcgcccc cgacacctgg tagccgacgg
     4501 tgagcagggc ggcaccgccg atcgacgcga tggcaccgac catgcgacgg ccgcggatgt
     4561 cggccagccg cccgccgacg acgatcccga tgccgccggg tgtgttcgtg gcgagggtga
     4621 agaccgtgag ctcgatggcg gtgaagccgt gctcggtgcg caggaactcg ttctggaact
     4681 ggctgacggg ggcgaggaag accagtccgg cgaagctggc ggccgcgagg agcgccaggc
     4741 ggcggcgatg gcccttcatc gtggcccggc cgtgcggctt cacgaaccgc cggctctcgg
     4801 ggatccggcg agccaggcgg atgaagaacg gcagccccag cagcggtacc acgtagatga
     4861 tccgccaggc gtaccggccc atgtcggcga cgggcaggag gatgaccgcc atgccggcgc
     4921 cgagggcggc ggtcatggcc agcacgctgg ctgcgtaggc ccggccgccc gctggcatct
     4981 cctcgacgga caccacgccg atcagcagcg ccagggcggt ggcgaacgcc cgcgccacgg
     5041 tctgggtgcc gcccagggtg atcaggtcgg gcgacgcggc gccggcgacc gatgccgcca
     5101 caccgccgac ggcgcacacg agcaggagct tctggcggcc ccgccggtcc gagatcgtga
     5161 ccagcacgag cgagagcagc accccgaccc gaaccgtggc caaggtggcg ccctgggccg
     5221 cggtggaggc gccgaactcc ttggcggcgt aggtcatcgt ctgggtgatg gtcgtgccga
     5281 ggtagccggt gagcatcgac agcccgcaca gcatcgccag gaccgacgac gagcgtgcgt
     5341 ccaaccgagc tggcggagac caccagggcg ctcgtcgggc cgcctcgtcc tcgtcgagga
     5401 tctcgggcgg cccggtgcgg cgcagttgcc gcgccacgag cggtcggaac agcgctcccc
     5461 acagcgggat ggcgacgtcc caggtgatct cctcgcggac gtggtagccg ccggcgtcga
     5521 tgcggtcggc gtgcaccgtg cgctcgtagg cggagaacgg cccctgctcg cacacgaagc
     5581 ggaagtcgtg gccgtcggga tcggcggcgt cgaggtcctc gaggaggatg tcgtcgcgcg
     5641 ggacgacgat ggcgtgcagg ccggcctcgt cgacgtcgag ctcgttgacc gtggtccggg
     5701 gcgtcacggg atggtcgctc cctcgtcggg cggggtgggg tcgtcatctt ggggtgcgtg
     5761 gcgcttggcg gcgacccaca gcagcccccc gaacagtccc accggcacga tcaccatgag
     5821 cgcctcatcc cagccgccct ggtgggcgag caccccggcg tagcccagca cggcgccagc
     5881 atcccagatg caggggaaca ccgacgacct cccgtcggtt gtagcccgtg gggttcgccc
     5941 gcgccggaac ggtcgtgaga ggatctcgac acccagagga gcatcgtgga agaccaggta
     6001 cgcaagacca tcgaggtgat ccgcccggcc atccaggccg acaacggcga catcttcctg
     6061 aagggcgtcg acgaggccac cggcgtggtc accgtcgagc tcaccggcgc gtgcgtctcc
     6121 tgtccggcgt ccacggtcac cctcaaggcc ggcgtcgagc gcatcttgaa ggaccgggtc
     6181 ccgggcgtca cggccgtcga gcagttcggt gccgaaccgg ccgagaccga gtcggccgtc
     6241 tccctctgat cgatccggca agatctctgc gtgaccacct cggaccagct cgacgcggcg
     6301 cgccgcggtg accgccggac gctggcccgg ctcctgtcgt cgatcgagcg cggcgacgct
     6361 gcctcgcacg acatcaaccg gaccgtgttc agccaggtcg gcggggccta cacggtgggt
     6421 ctcaccgggg cgcccggtgc cggcaagtcc acgctgacca gcgccgcggt ggcctcggcc
     6481 cgccgtcggg gggagcgggt gtcggtgctc gccatcgacc cgtcgtcccc gttcagcggc
     6541 ggcgccctcc tgggcgaccg cacccgcatg aacgaccacg ccctcgacga cggcgtgttc
     6601 atccggtcga tggccacgcg cggccacctc ggcgggttgg cgctcgccac ccgcgaggcg
     6661 atccgcctcc tcgatgccgt cggcttcccg tgggtgctgg tggagaccgt gggcgtgggc
     6721 caggtcgaag tcgaggtcgc cggggccgcc gacaccaccg tcgtcgtcgt gaacccgggt
     6781 tggggcgatg ccgtcc
//
LOCUS       DATMFS010000360         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_221648, whole genome shotgun sequence.
ACCESSION   DATMFS010000360 DATMFS010000000
VERSION     DATMFS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221648"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VNQ33_06630"
     CDS             <1..1085
                     /locus_tag="VNQ33_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HWJ97818.1"
                     /translation="EFVEHFFEFIAEEVRELLASLGFRSLQEAIGHAEVIDTSGAIDH
                     WKASGLDLTPILHVPERPFGDELHNTKGQDHGLDRALDQQLILAARSAIDTGEPVKLE
                     FPIRNVNRTVGTMLGHEVTKRHGGLGLPDDTIDITLRGAAGQSVGAFLPKGVTLRLFG
                     DANDYAGKGLSGGRLVVRPSEDVPAGFVAEDNIIAGNVLLYGATAGEAFFRGRAGERF
                     CVRNSGATAVVEGVGDHGCEYMTGGRAVILGSTGRNFGAGMSGGIAYVWDPDGTFPAL
                     LNDEMVDLDPLDDLDTSWLVTAIFRHQAETGSEVAGRILSDWQYTVHQFVKVMPRDYK
                     RVLSAIKGAEESGTDIDDAIMAAARG"
     gene            1091..>2336
                     /locus_tag="VNQ33_06635"
     CDS             1091..>2336
                     /locus_tag="VNQ33_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWJ97819.1"
                     /translation="MGDVQGFLKHGRELPSRRPVPVRIKDWKEVYEPFGDGKLETQAS
                     RCMDCGIPFCNNGCPLGNLIPDWNDLVYRDCWQEAIERLHATNNFPEFTGRLCPAPCE
                     AACVLGINQDPVTIKQVEVSIIDKAWEEGWVQPIRPDVKTGKKVAVVGSGPAGLAAAQ
                     QLTRAGHDVVVFERADRIGGLLRYGIPEFKMEKRHLDRRIDQMRAEGTEFRTGVDVGT
                     DLTADDLRAEFDAVVLAGGATAARDLPIPGRELEGIHQAMEYLPLANRVQEGDLPASP
                     IDVAGKHVVIIGGGDTGADCLGTAHRQGAASISQFEIMPKPADSRPDHTPWPTWPLIY
                     RTASAHEEGGDRVFSVNTECFQDDGNGRVKALRAHEVKLVDGRFEKVEGTDFELPADF
                     VFLSMGFTGPESNWITEQFGVEL"
BASE COUNT          369 a          873 c          782 g          312 t
ORIGIN      
        1 ccgagttcgt cgagcacttc ttcgagttca tcgccgagga ggtccgcgag ctgctcgcca
       61 gcctcgggtt ccgctcgctc caggaggcca tcggccacgc cgaggtcatc gacaccagcg
      121 gggccatcga ccactggaag gcgtcgggcc tcgacctcac gcccatcctc cacgtgcccg
      181 agcggccctt cggcgacgag ctccacaaca ccaagggcca agaccacggc ctggaccggg
      241 cgctcgacca gcagctgatc ctcgccgccc ggtcggccat cgacacgggc gagccggtga
      301 agctcgagtt cccgatccgc aacgtgaacc ggacggtcgg caccatgctc ggccacgagg
      361 tcaccaagcg ccacggcggg ctcggcctgc ccgacgacac catcgacatc acgctgcgcg
      421 gcgcggccgg ccagagcgtc ggggcgttcc tgcccaaggg cgtgaccctg cgcctcttcg
      481 gcgacgccaa cgactacgcc ggcaagggcc tctcgggcgg ccgcctcgtc gtgcgcccgt
      541 ccgaggacgt acccgccggg ttcgtggccg aggacaacat catcgccggc aacgtgctcc
      601 tctacggcgc cacggccggc gaggccttct tccggggccg ggcgggggag cggttctgcg
      661 tccgcaactc cggcgccacc gcggtggtgg agggcgtggg cgaccacggc tgcgagtaca
      721 tgaccggcgg ccgggccgtg atcctcggct ccaccggccg caacttcggc gccggcatgt
      781 cgggcggcat cgcctacgtg tgggaccccg acggcacgtt cccggccctc ctcaacgacg
      841 agatggtcga cctcgacccg ctcgacgacc tcgacacctc gtggctggtg acggcgatct
      901 tccgccacca ggccgagacc ggctccgagg tggccgggcg catcctgtcg gactggcagt
      961 acaccgtgca ccagttcgtg aaggtcatgc cccgcgacta caagcgggtc ctctcggcca
     1021 tcaagggcgc cgaggagtcc ggtaccgaca tcgacgacgc catcatggct gcggcaaggg
     1081 gctgatccag atgggtgacg ttcaagggtt cctcaagcac ggccgggagc tgccctcgcg
     1141 gcggcccgtg cccgtccgca tcaaggactg gaaggaggtc tacgagccgt tcggcgacgg
     1201 caagctcgag acccaggcca gccggtgcat ggactgcggc atcccgttct gcaacaacgg
     1261 ctgcccgctg ggcaacctga tcccggactg gaacgacctc gtctaccggg actgctggca
     1321 ggaggccatc gagcgcctcc acgccaccaa caacttcccc gagttcaccg gccgcctctg
     1381 cccggcgccg tgcgaggccg cctgcgtcct cggcatcaac caggacccgg tgaccatcaa
     1441 gcaggtcgag gtctcgatca tcgacaaggc gtgggaggag ggctgggtcc agcccatccg
     1501 ccccgacgtg aagaccggca agaaggtcgc cgtcgtcggc tccggcccgg ccggcctggc
     1561 cgccgcccag cagctcaccc gggccggcca cgacgtcgtc gtgttcgagc gggccgaccg
     1621 catcggcggg ctcctgcgct acggcatccc cgagttcaag atggagaagc gccacctcga
     1681 ccggcgcatc gaccagatgc gcgccgaggg caccgagttc cgcaccggcg tcgacgtggg
     1741 caccgacctc accgcggatg acctgcgggc cgagttcgac gcggtcgtgc tcgccggcgg
     1801 cgccaccgcg gcccgcgacc tcccgatccc gggccgtgag ctcgagggca tccaccaggc
     1861 catggagtac ctcccgctcg ccaaccgcgt gcaggagggc gacctgcccg cctcgcccat
     1921 cgacgtggcc ggcaagcacg tcgtcatcat cggcggcggc gacaccgggg ccgactgcct
     1981 cggcaccgcc caccgccagg gggcggcctc gatctcccag ttcgagatca tgcccaagcc
     2041 cgcggacagc cggccggacc acaccccgtg gcccacctgg ccgctcatct accgcaccgc
     2101 ctcggcccac gaggagggcg gcgaccgcgt gttctccgtg aacacggagt gcttccaaga
     2161 cgacggcaac gggcgggtca aggcgctgcg ggcccacgag gtgaagctgg tcgacgggcg
     2221 cttcgagaag gtggagggca ccgacttcga actgccggcc gacttcgtgt tcctctcgat
     2281 gggcttcacc ggcccggagt cgaactggat caccgagcag ttcggcgtgg agctcg
//
LOCUS       DATMFS010000361         5204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_315505, whole genome shotgun sequence.
ACCESSION   DATMFS010000361 DATMFS010000000
VERSION     DATMFS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315505"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VNQ33_06640"
     CDS             <1..235
                     /locus_tag="VNQ33_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWJ97820.1"
                     /translation="AVGWVDADRRCGSLNVAIRTFWFADGEIRFGTGGGITWDSDPDG
                     EWEETELKARRLLRVAAGSEVPPLTATDHTGAR"
     gene            232..1092
                     /locus_tag="VNQ33_06645"
     CDS             232..1092
                     /locus_tag="VNQ33_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWJ97821.1"
                     /translation="MSTWVWVDGNLSPAGDARVAALDHGVTVGDGVFETCKVVDGTPF
                     ALTRHLARLRRSAEIIGLRLPWTDGALRAACDEAIGAAAIDAANATAGVQRLRITATG
                     GPGPLGSDRSDVPPTLVIAAGPGSPWPATTSVATVPWVRNERSAVAGAKTTSYAENVV
                     ALAEAHRHGASEALLANTVGALSEGTGSNVFLELDGMLCTPSLATGCLPGITRALVCE
                     TVDVVERDDLRFDDLRRSNEAFLTSSTRDIHPIDRIDGAPLARSAPGPLTTAAIEALG
                     ALQARTLDPV"
     gene            1089..3245
                     /locus_tag="VNQ33_06650"
     CDS             1089..3245
                     /locus_tag="VNQ33_06650"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWJ97822.1"
                     /translation="MSAPRAAGTALLLALALLAAACSTGGGSEGAADPGGSATTTEDP
                     SMGGPTTSGPLGSGTSPLREGLRIEVVSSQPDRVSGDDARIRVTPAPGTAASTVRVQV
                     NDVDATAALAVADGHLEGVVHGLIEGNNTVKATAGGTEVIQHIRAWPLTGPVISGPHL
                     PLLACSTEEQGLGAPLDDDCSAPVSTTWRYITTKGAIADLPSRSTPPKDLARAGVDGA
                     EVPLYIRTERGVINRAVYEIASIDATPADDDPTGPGWSKRLVYRFGGGCGTTYGQGAL
                     PTGGDDAGLLRQGYAVATASFNNFDVQCNDVLSAETAMMVKERVIERFGVPDLTIGEG
                     REGGAAQLHLLVQNYPGLVNGVVASSPLPDVASVWSGVTDCGLLLHYYASAKGTSLSD
                     AQRTAINGHATAKTCQTWADTWLGAIDPTDGCDPKIPASAVYDPETNRGGLRCTLQDA
                     NRNQFGTDPATGWAARPLDNVGVQYGLQALNEGTITFDEFAALNAGIGGYDIDGRITQ
                     AREEADPEAVLHAYETGRVAMGGGDLVAVPIIDIDVFDDPTGATADRFRAFSLLDRLA
                     GGTPESVPGFQIWTASPKDHDPLDAIAVMDRWLTALRDDAAGGSIDAVLFRNRPGDAV
                     QDCGTDGACADRFPISGDPRTVAGAPRSDQVVKCALKPVDPMDYQPELTADQLRKLQG
                     VFVQGVCDWSGPSVGELVPANPDRSYEDVPSPNQSA"
     gene            complement(3389..4246)
                     /locus_tag="VNQ33_06655"
     CDS             complement(3389..4246)
                     /locus_tag="VNQ33_06655"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWJ97823.1"
                     /translation="MDDRPAGDLARSSLVGTRFSDVRWVAETGSTNADALAMARHGEA
                     EGVVLVADHQTAGRGRLGRTWQAPAGGSLLCTILLRPPASIAPATTMAVAVAAATAVE
                     DLTGRSPRLKWPNDLVWPGDGSAADRKLAGILAEVDWPAASQISSGWREPRPQDRLVV
                     AVGIGINVAWGDDVPPDLADLAVAIDQLTGPDAAPDREDLLVALLRHLDRRYAALLDG
                     PGGAAGLRDEWRRRSATLGRRVRIDLGADDLEGTAVDITEEGHLVVETLEGGRRTVAV
                     GDVIHLRPL"
     gene            4342..>5204
                     /locus_tag="VNQ33_06660"
     CDS             4342..>5204
                     /locus_tag="VNQ33_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWJ97824.1"
                     /translation="MAVVLTKILIANRGEIAVRVIRACQELGIASVAVYSELDRDALH
                     VRLADEAYALGGQTAAESYLNTEAIIDVIERSGADGVHPGYGFFSENTDFARAITERG
                     VTFIGPPPESIEVMGDKVSSRIAAEAADVHGVPGTTEFLTSGDEVVAFGDEHGWPVAI
                     KAAFGGGGRGMRVVESAADAHTALASAQSEALKGFGRDECYVERFLVKPRHVEMQIIG
                     DQHGNCVWIGERDCSVQRRHQKLIEEAPAPNFPPEIRQAMGEAAVRVAKACGYYNAGT
                     VEFLYDDGAFY"
BASE COUNT          732 a         2017 c         1799 g          656 t
ORIGIN      
        1 ggccgtcgga tgggtcgacg cggaccggcg gtgcggctcg ctcaacgtgg ccatccgcac
       61 cttctggttc gccgacggcg agatccgctt cggcaccggc ggagggatca cgtgggactc
      121 cgaccccgac ggcgagtggg aggagaccga gctcaaggcc cggcgcctgc tccgggtcgc
      181 cgccggatcg gaggtcccgc cgctgaccgc caccgaccac actggagcgc gatgagcacc
      241 tgggtctggg tcgacggcaa cctctccccc gctggcgacg cacgcgtcgc cgcgctcgat
      301 cacggggtca ccgtgggcga tggggtgttc gagacctgca aggtggtcga cggcacgccg
      361 ttcgccctga cccgccacct cgcccggctg cggcgctccg ccgagatcat cggcctgcgc
      421 ctcccctgga ccgacggtgc cctgcgcgcc gcctgcgacg aggccatcgg cgccgccgcc
      481 atcgatgcgg ccaacgccac cgccggggtc caacggctcc gcatcacggc caccggcgga
      541 cccgggccgc tcggttcgga ccgcagcgac gtgccgccga ccctcgtcat cgcggccggg
      601 cccggcagcc cgtggccggc caccacgtcc gtggccaccg tgccctgggt gcgcaacgag
      661 cgcagcgccg tcgccggcgc caagaccacc tcctacgccg agaacgtggt ggccctcgcc
      721 gaggcccacc gccacggcgc gtcggaggcg ctgctggcga acaccgtcgg cgcgctgtcg
      781 gaggggaccg gctccaacgt cttcctcgag ctcgacggga tgctctgcac gccctcgctc
      841 gccaccggct gcctgcctgg catcacccgc gctctcgtgt gcgagaccgt cgacgtcgtc
      901 gagcgcgacg acctccgctt cgacgacctc cgccgctcga acgaggcgtt cctcacctcg
      961 tccacccgcg acatccaccc gatcgatcgg atcgacggcg caccactggc ccggtccgca
     1021 ccagggcccc tcaccaccgc ggcgatcgag gcgctgggcg cgctccaggc ccggacgctg
     1081 gacccggtgt gagcgcgccg cgcgcggccg gcaccgccct cctcctcgcc ctcgcgctgc
     1141 tcgcggccgc ctgctccacc ggaggcggga gcgagggcgc cgcggacccg ggcgggtcgg
     1201 ccaccaccac cgaggacccg tcgatgggcg gaccgaccac cagcggcccc ctcgggagcg
     1261 gcacgtcgcc gctgcgcgag ggcctgcgga tcgaggtcgt gagcagccag cccgaccggg
     1321 tctcaggcga cgacgcccgc atccgggtca cgccggcgcc gggcaccgcc gccagcacgg
     1381 tgcgggtcca ggtgaacgac gtcgacgcca ccgctgcgct cgccgtcgcc gacgggcacc
     1441 tcgaaggcgt ggtccacggc ctcatcgaag gcaacaacac cgtgaaggcg acggccggag
     1501 ggaccgaggt catccagcac atccgggcct ggccgctgac cggcccggtc atctccggcc
     1561 cgcacctgcc gctgctcgcc tgctccaccg aggaacaggg cctcggggcg ccgctcgacg
     1621 acgactgctc ggccccggtg tccacgacgt ggcgctacat cacgaccaag ggagcgatcg
     1681 ccgacctgcc gagccggagc accccgccga aggacctcgc ccgggcgggg gtcgatggcg
     1741 ccgaggtgcc gctctacatc cgcaccgagc gcggggtcat caaccgggcc gtgtacgaga
     1801 tcgcgtcgat cgacgccaca ccggccgacg acgacccgac cggacccggc tggagcaagc
     1861 ggctcgtcta ccggttcggc ggcgggtgcg gcaccaccta cggccagggc gcgctcccga
     1921 ccggaggcga tgatgccggc ctcctccgcc agggctacgc cgtcgccacg gcgtcgttca
     1981 acaacttcga cgtgcagtgc aacgacgtcc tctcggccga gacggcgatg atggtgaagg
     2041 agcgggtgat cgagcgcttc ggcgtccccg acctcaccat cggcgagggc cgtgagggcg
     2101 gtgccgccca gctccacctg ctcgtccaga actaccccgg cctggtgaac ggcgtggtcg
     2161 catcgtcgcc cctccccgac gtggcgtcgg tctggtccgg cgtgacggac tgcgggctgc
     2221 tgctgcacta ctacgcgtcg gcgaagggca cgtcgctcag cgatgctcag cgcacggcca
     2281 tcaacgggca cgccaccgcg aagacgtgcc agacgtgggc cgacacctgg ctgggcgcca
     2341 tcgacccgac cgacggctgc gacccgaaga tccccgcctc ggccgtgtac gacccggaga
     2401 cgaaccgcgg cgggctccgc tgcacgctgc aggacgccaa ccgcaaccag ttcggcaccg
     2461 acccggccac cggctgggcc gcccgccccc tcgacaacgt gggcgtccag tacggcctcc
     2521 aggcgctcaa cgagggcacg atcaccttcg acgagttcgc cgccctcaac gcgggcatcg
     2581 gcggctacga catcgacggc cggatcaccc aggcccgcga ggaggccgat cccgaggccg
     2641 tcctgcacgc ctacgagacc ggtcgcgtcg ccatgggcgg cggcgacctg gtggcggtcc
     2701 cgatcatcga catcgacgtg ttcgacgacc ccaccggcgc caccgccgac cggttccgcg
     2761 ccttctcgct gctcgaccgg ctggccggag gcacgcccga gtccgtcccc ggcttccaga
     2821 tctggaccgc ctccccgaag gaccacgacc cgctcgacgc catcgccgtg atggaccggt
     2881 ggctgaccgc cctccgcgac gacgctgccg gcgggtcgat cgacgcggtc ctcttccgca
     2941 accggcccgg ggacgcggtg caggactgcg gcaccgacgg cgcgtgcgcg gaccggttcc
     3001 cgatctccgg cgacccccgg accgtggctg gcgcgccccg ctcggaccag gtcgtcaagt
     3061 gtgcgctcaa gccggtggac cccatggact accagcccga gctcaccgcc gatcagctgc
     3121 ggaagctgca gggcgtgttc gtccagggcg tgtgcgactg gtcgggcccc tcggtcggcg
     3181 agctggtccc cgccaacccc gatcgctcct acgaggacgt cccctccccc aaccagagcg
     3241 cctgacaccg ccacccggtc gctcgcaccc ctccacgaac ctcgtgtgcg agagggccga
     3301 tctgggtgtg ggcagacctg ggcgtgccca cctccggtgt ccgccctggt gccccgaccc
     3361 tcacggagct cgtctcggtc ggggcggatc agagggggcg gaggtggatg acgtcgccga
     3421 cggcgacggt gcggcgaccg ccttcgaggg tctcgaccac gaggtggccc tcctcggtga
     3481 tgtcgacggc cgtgccctcg aggtcgtccg cgcccaggtc gatgcgcacc cggcgcccga
     3541 gcgtggccga gcgccggcgc cactcgtcgc gcaggcccgc cgcgccgccc ggaccgtcga
     3601 gcagggcggc gtagcgccga tcgaggtgac ggagcagggc gacgagcagg tcttcccgat
     3661 ccggtgccgc atccgggccg gtcagctggt cgatggcgac ggcgaggtcg gcgaggtcgg
     3721 gcggcacgtc gtcgccccag gcgacgttga tgccgatccc gacggccacc acgagccggt
     3781 cctggggccg gggctcgcgc cacccgctcg agatctggct ggccgccggc cagtcgacct
     3841 cggccaagat gccggcgagc ttgcggtccg cggctgatcc gtcaccgggc cagaccaggt
     3901 cgttcggcca cttcagccgc ggcgagcgac cggtgaggtc ctcgacggcc gtggcggcgg
     3961 cgacggccac cgccatggtg gtcgccggtg cgatggacgc cggcggccgc aacaggatcg
     4021 tgcacagcag cgacccgccg gcgggggcct gccacgtgcg gccgagtcgg ccccgacccg
     4081 ccgtctggtg gtccgcgacg aggaccacgc cctcggcttc gccgtgcctc gccatcgcca
     4141 gcgcatcggc gttggtggaa cccgtctcag cgacccaccg gacgtcggag aaacgggttc
     4201 cgaccagcga cgaacgtgcg agatctcctg ccgggcggtc gtccacgaac ggcagtccac
     4261 cctgaaccgg agggcgcggg caaggccgcc cgcgggtgcc gcgcgcgtca tctggcactc
     4321 gcacccgccg acgccgtaga catggcggtt gtgctgacca agatcttgat cgcgaaccgg
     4381 ggcgagatcg ccgtccgggt catccgtgcc tgccaggagc tggggatcgc ctccgtggcg
     4441 gtgtactccg agctcgaccg agatgcgctg cacgtgcgcc tcgccgacga ggcctacgcg
     4501 ctgggcggcc agacggcggc cgagagctac ctcaacaccg aggccatcat cgacgtcatc
     4561 gagcgttccg gcgccgatgg cgtgcacccg ggctacggct tcttctcgga gaacaccgac
     4621 ttcgcccggg ccatcaccga gcgcggcgtc acgttcatcg gcccaccgcc cgagtcgatc
     4681 gaggtcatgg gcgacaaggt ctccagccgc atcgccgccg aggccgccga cgtgcacggc
     4741 gtgcccggca ccaccgagtt cttgacctcg ggcgacgagg tcgtcgcctt cggcgacgag
     4801 cacggctggc cggtcgccat caaggcggcc ttcggcggcg gcggccgcgg catgcgcgtc
     4861 gtcgaatccg ccgccgacgc ccacaccgcc ctcgcgtcgg cgcagtccga ggccctgaag
     4921 ggcttcgggc gcgacgagtg ctacgtcgag cgcttcttgg tcaagccccg ccacgtcgag
     4981 atgcagatca tcggcgacca gcacggcaac tgcgtctgga tcggcgagcg cgactgctcc
     5041 gtgcagcgcc gccaccagaa gctcatcgag gaggccccgg cccccaactt cccgcccgag
     5101 atccgccagg ccatgggcga ggcggccgtc cgcgtcgcca aggcgtgcgg ctactacaac
     5161 gccggcacgg tcgagttcct ctacgacgac ggcgccttct actt
//
LOCUS       DATMFS010000362         5630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_82384, whole genome shotgun sequence.
ACCESSION   DATMFS010000362 DATMFS010000000
VERSION     DATMFS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82384"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VNQ33_06665"
     CDS             <1..324
                     /locus_tag="VNQ33_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97825.1"
                     /translation="LARDLPAPVESVHLRAWPADVPTDLATLRGAPWEARADAVMYRT
                     ALADLAAERGWPVAVYDAKRAEADAGALLGDRAHAVLHGPRAALGAPWAKDHRVALAA
                     TITGS"
     gene            420..2096
                     /locus_tag="VNQ33_06670"
     CDS             420..2096
                     /locus_tag="VNQ33_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ97826.1"
                     /translation="MNPVILLVCDENADNLHAEFGRYARDYEVRVARSADGAVAVAME
                     VGTAGGRLALVVADTALFARTDPDDPHLAALVTLHGWRQIVPTARTLVVSPFERFLVD
                     SEALRAGQAKGKFDALLLMPRGARDEEFHGAVTELLSDWGSTVADPEVEAVRVVADPT
                     EALTLDLRDLLVRMGMPHRIHPPESDVGQELIAAFDGPPDLPLVEVLSRGVIHVRTTR
                     ELSAAFYGRPDEVEDDEVFDLCVVGAGPSGLAAAVYGASEGLSTVVLECDAIGGQAGA
                     SSMIRNYLGFPRGISGMRLAQRARSQAARFGARFITGWDVAGLTPAAGGRPHVIRTEG
                     GDLRSRAVVIATGAAYRKLQVPAIGALTGRGVFYGSAMTAAREMEGLDVVVVGGGNSA
                     GQAALHLARFARSVTIAVRRDGLEATMSRYLIDEIEYSPRVVVRPCSEVIDGGGAGRL
                     EWIDLCDTVTKVAERRRCDGLFLLIGAEPRCEWLPSDLALDDRGFVLTGRDVPQDAWV
                     GGLPPESLATSVRGVFAVGDVRSGSMKRVAAASGEGSSVVPLVHAYLETL"
     gene            complement(2220..2699)
                     /locus_tag="VNQ33_06675"
     CDS             complement(2220..2699)
                     /locus_tag="VNQ33_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWJ97827.1"
                     /translation="MSEAAAKAFERFQADVGNDEGTGEWFQVTQEQIDQFADVTFDHQ
                     FIHVDPEAAKATPFGATIAHGFLTLSMLTHLSAGTSGPPDPKKFEGFLMGVNYGFNKV
                     RFVNPVKVGSRIRATAVTSNVELKGNSVDATRNFTVEIEGEEKPALVAEWVTRMVYA"
     gene            complement(2895..3302)
                     /locus_tag="VNQ33_06680"
     CDS             complement(2895..3302)
                     /locus_tag="VNQ33_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ97828.1"
                     /translation="MADGVLHLSIPVRDLDEARSFYEEALGCEVGRVRDDWLDVWFFG
                     LQLTLQHRPDEVAGVEGQGVRHFGVALDDADVFTVLVQRLRDHQVEWLAEPKVHTAAE
                     LSGKVAGKLADPSGNVIELKYYDDPAGLRPGAS"
     gene            3459..4115
                     /locus_tag="VNQ33_06685"
     CDS             3459..4115
                     /locus_tag="VNQ33_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWJ97829.1"
                     /translation="MEPGLVAEPVVERVDLDATSWVDVVRGFVTGADAVHDAVREQTR
                     WSEERVFRYDHWVEVNRLGAFWRRGDPVAHPVLLDAHRWLQHRYRVPFEGFSLVRYRD
                     GRDGQGFHRDREMRWLDETVIGVLTLGAQRPWLLRPRANRNRHDLENRGATHDLSPAS
                     GDLVVMGGRCQADWEHSVAPVPHQHVGDRISLQWRWTSRRGRPERGGNWGDPRRYGKG
                     "
     gene            complement(4179..5042)
                     /locus_tag="VNQ33_06690"
     CDS             complement(4179..5042)
                     /locus_tag="VNQ33_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HWJ97830.1"
                     /translation="MDRAHRPRATLLPAQDDTRAARRPHRLQSVATASDRAAARGSDD
                     APEVTARFLAYRESGDRSIRNELVEHHRSLADALARRYAERGEPLDDLVQVALLGLVK
                     AVGRFDPDKGIPFAGFAVPTITGELRRHFRDHTWAVKVHRRAKDLHVRIPAVSDRLTA
                     ALGRTPTPAELAEETGCSIDELIEALDAGTAYRTTSTDTVEGGLAASYALDRSAGVPS
                     FEPEDRLQLARLLAELSERDRTIVGLRYFEDLSQSEIAERVGVSQVHVSRLLRAALGQ
                     MRSSLAEQAAG"
     gene            complement(5183..>5630)
                     /locus_tag="VNQ33_06695"
     CDS             complement(5183..>5630)
                     /locus_tag="VNQ33_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWJ97831.1"
                     /translation="GRGPSSMAPTLVVTGIDLVVDGLLLGIAFSDGGAVGPLLTLALT
                     MEMLGLGVAVALVLIAAGVGSVRTVAMTFALSCCVLLGGVLGASTLSNLGADALSVVL
                     AFATAALLYLVTEELLTEAHETEDTPLLTATFFVGFLLILVLSFNA"
BASE COUNT          737 a         2076 c         2045 g          772 t
ORIGIN      
        1 ctcgcccgtg acctcccagc ccccgtcgag tcggtgcacc tgcgggcgtg gccagcagac
       61 gtccccaccg acctcgccac gctgcgcggc gcgccctggg aggcccgagc cgatgccgtg
      121 atgtaccgca cggccctcgc cgacctggcc gcggagcgcg gctggccggt ggcggtctac
      181 gacgccaagc gggcggaggc cgatgccggg gcgctcctcg gcgaccgcgc ccatgccgtg
      241 ctccacggcc cgcgcgccgc cctgggtgcc ccctgggcga aggaccaccg cgtcgcgctc
      301 gccgccacca tcaccggctc ctgagcatcc cggacgtccc cgcccgcccg gcgctggggg
      361 ggggcgtcgg tgaccggcac cagcccccag ggttgcggtg cccgggcata ctgcccggca
      421 tgaacccggt catcctcctc gtctgcgatg agaacgcgga caacctgcac gccgagttcg
      481 gccggtacgc ccgggactac gaggtccgcg tcgcccgttc cgcggatggt gccgtggccg
      541 tcgccatgga ggtcggcacc gccggcggcc gacttgcgct ggtggtggcc gacaccgccc
      601 tgttcgcccg gaccgacccg gacgacccgc acctcgccgc gctggtcacg ctgcacgggt
      661 ggcgccagat cgtcccgacg gcccgcaccc tggtggtctc gcccttcgag aggttcctcg
      721 ttgacagcga ggcgctgcgc gccggccagg ccaagggcaa gttcgacgcc ttgttgctga
      781 tgccccgagg cgcccgcgac gaggagttcc acggcgcggt gaccgaactg ctctcggact
      841 ggggctcgac ggtggccgac cccgaggtcg aggccgtgcg cgtcgtcgcc gatccgaccg
      901 aggcgctgac gctcgacctc cgcgacctcc tggtgcgcat gggcatgccc caccggatcc
      961 acccgccgga gtccgatgtc ggccaggagc tcatcgccgc cttcgacggt ccgccggacc
     1021 ttccgctggt ggaggtgctg tctcgaggcg tgatccacgt tcgcaccacc cgggagctgt
     1081 cggctgcgtt ctacggacgg cccgacgagg tggaagacga cgaggtcttc gacctgtgcg
     1141 tggtcggcgc cggcccctct ggcctggcgg cggcggtgta cggcgcatcc gaggggctgt
     1201 ccaccgtcgt gctcgagtgc gatgccatcg gcggccaggc cggggccagc tcgatgatcc
     1261 gcaactacct cgggttcccc cgtggcatct cggggatgcg gctcgcccag cgggcccggt
     1321 cccaggccgc tcgcttcggt gcccggttca tcaccgggtg ggatgtggcc ggcctcaccc
     1381 cggccgccgg tggacgcccc cacgtgatcc gcaccgaggg cggcgacctc cgttcccggg
     1441 ccgtggtgat cgccaccggg gcggcctacc ggaaactgca ggtcccggcg atcggggcct
     1501 tgaccggccg cggcgtgttc tacggcagcg ccatgaccgc ggcccgcgag atggagggcc
     1561 tcgacgtcgt ggtggtcggc ggcggcaact ccgccggcca ggccgccctc cacctcgccc
     1621 ggttcgcccg cagcgtgacc atcgccgtgc gccgcgacgg gctggaggcg acgatgtcga
     1681 ggtacctgat cgacgagatc gagtacagcc cccgggtcgt cgtccggccc tgctccgagg
     1741 tgatcgacgg cgggggcgcc ggccgcctcg agtggatcga cctctgcgac acggtcacga
     1801 aggtcgccga gcgccgccgc tgcgacggct tgttcctcct gatcggcgcc gagccccggt
     1861 gcgagtggct gccgtcggac ctcgccctcg acgaccgcgg cttcgtgctg accggccgcg
     1921 acgtgcccca ggacgcctgg gtcggtggcc tgccgccgga gagcctggcc acgtcggtcc
     1981 ggggcgtctt cgccgtcggc gacgtgcgct ccggatccat gaagcgggtg gctgcggcca
     2041 gcggcgaggg ctcgagcgtc gtccccctcg tccacgccta cctcgagacc ctctgagcgg
     2101 tccacccgct caccctgcgg ggtcgcgagc ggcgtggctc ggcacctccg agcaggacca
     2161 tcagggagcg gctggcgcgc ccgccgcccg accggggaac ggcagggacc cgagagccct
     2221 caggcgtaga ccatgcgggt gacccactcg gcgacgagcg ccggcttctc ctcgccctcg
     2281 atctccaccg tgaagttgcg ggtcgcgtcg accgagttgc ccttcagctc cacgttggag
     2341 gtgacggcgg tggcccggat gcgggagccg accttgaccg ggttcacgaa ccggaccttg
     2401 ttgaacccgt agttcacgcc catgaggaag ccctcgaact tcttcgggtc cggcgggccg
     2461 ctggtgccgg ccgacaggtg ggtcagcatc gacagggtca agaacccgtg ggcgatggtg
     2521 gcgccgaacg gcgtcgcctt ggcggcctcg gggtccacgt ggatgaactg gtggtcgaac
     2581 gtgacgtccg cgaactggtc gatctgctcc tgcgtgacct ggaaccactc gccggtgccc
     2641 tcgtcgttgc cgacatcggc ctggaaccgt tcgaacgcct tggctgctgc ttcgctcatg
     2701 cccacagtcc tagccgctcc cccgcggccg atgcgacggc gaccggatcc tcggcccggc
     2761 gggcaggctg ctgctcgtgg cggcaccgga cccggacccc gctcgccagc tgcgagggcc
     2821 ggtggaacgg ccccgggccg acctcgacgg aggcctgacg ccgggccggg gcggcgaggc
     2881 aggcctcggg ggcgtcagct cgcgccgggt cggagcccgg cggggtcgtc gtagtacttg
     2941 agctcgatca cgttgccgct gggatcggcg agcttgccgg ccaccttccc gctcagctcg
     3001 gccgccgtgt ggaccttcgg ctcggccagc cactcgacct ggtggtcgcg gaggcgctgc
     3061 acgagcaccg tgaacacgtc ggcgtcgtcg agcgccacgc cgaagtgccg gacaccctgc
     3121 ccttcgacgc cggcgacctc gtcgggccgg tgctggagcg tgagctggag gccgaagaac
     3181 cacacgtcga gccagtcgtc gcgcacccgc ccgacctcgc acccgagcgc ctcctcgtag
     3241 aacgacctcg cctcgtcgag gtcgcggacc gggatcgaga ggtgcagcac gccatcggcc
     3301 atgccgccac cctgccacga ccgccgctcc gagcgggtgt cccctttccg ttgaccggtc
     3361 cagagcccca gcaggcccgt cttgcacgag gagcctctgg atgccgtctc gaagctgctc
     3421 accgcccggg gccggaccgg gccggtaccg tcggatggat ggagccgggg ctggtcgccg
     3481 aacccgtcgt cgagcgcgtc gatctggatg ccacgtcgtg ggtggacgtg gtgcggggct
     3541 tcgtgaccgg tgccgatgcc gtgcacgacg ccgtccggga gcagacccgg tggagcgagg
     3601 agcgggtgtt ccgctacgac cactgggtcg aggtcaaccg gctcggcgcg ttctggcgcc
     3661 gcggcgatcc tgtcgcccat ccggtgctgc tcgacgccca ccgctggctc cagcaccgct
     3721 accgggtgcc gttcgagggc ttcagcctgg tgcgctaccg ggacgggcgc gacggccagg
     3781 gcttccaccg cgaccgggag atgcgctggc tcgacgagac cgtgatcggc gtcttgaccc
     3841 tcggcgccca gcggccgtgg ctgctgcggc cccgggccaa ccggaaccgc cacgacctcg
     3901 agaaccgcgg tgccacccac gacctctccc cggccagcgg cgatctcgtc gtgatgggcg
     3961 gccgctgcca ggccgactgg gagcactcgg tcgcccccgt cccgcaccag cacgtcggcg
     4021 accgcatctc gctccagtgg cggtggacat cccgccgggg ccggcccgag cgaggcggca
     4081 actggggcga cccccgccgc tacggaaagg gctgacgccg cggcccacca gccccgccgc
     4141 catgagcgac cggtccagcg accggcgcgg cgtcgggatc agccggccgc ctgctcggcg
     4201 agcgacgacc gcatctgccc cagcgccgct cgcagcaggc gcgacacgtg cacctggctg
     4261 accccgacgc gctcggcgat ctcgctctgg ctcaggtcct cgaagtaccg gaggcccacg
     4321 atcgtgcggt cgcgctcgga cagctcggcc agcagcctcg cgagctggag ccgatcctca
     4381 ggctcgaacg acggcacgcc ggccgagcgg tcgagcgcgt acgacgcggc gaggccgccc
     4441 tcgaccgtgt ccgtcgatgt cgtgcggtag gccgtgccgg cgtcgagggc ctcgatcagc
     4501 tcgtcgatgc tgcacccggt ctcctcggcg agctcggccg gggtcggcgt gcggccgaga
     4561 gcggcggtga gccggtcgga gaccgcgggg atgcggacgt ggaggtcctt ggcccgccgg
     4621 tggaccttca ccgcccaggt gtggtcgcgg aagtggcggc ggagctcacc ggtgatcgtc
     4681 ggcacggcga agccggcgaa cgggatgccc ttgtcggggt cgaagcgccc cacggccttg
     4741 accaggccga ggagggcgac ctgcaccagg tcgtcgagcg gctcgccgcg ctcggcgtag
     4801 cgacgggcga gggcgtcggc gagcgaccgg tggtgctcca cgagctcgtt gcggatcgag
     4861 cggtcgccgg actcgcggta cgccaggaag cgggccgtga cctccggcgc atcgtctgac
     4921 ccgcgggcgg cggcccggtc cgacgccgtc gccacgctct ggagccggtg aggccgccgg
     4981 gcggcacggg tgtcgtcttg ggcgggaagc agggtggcac gggggcggtg cgcacggtcc
     5041 atcaccgggg tcctctcgtc gtcgggccgg tggacgggac tacccagtcc tcacccgatc
     5101 cataccccgc ggacgccgca ccggcccgct gacctgctgt tccatcctcg tgggtgaagg
     5161 accgctcacc cggttcggcc gatcaggcgt tgaacgacaa gacgaggatc aggaggaagc
     5221 cgacgaagaa ggtggcggtc agcagcggcg tgtcctcggt ctcgtgagcc tcggtgagca
     5281 gctcctccgt gaccaagtag agcagtgctg ccgtggcgaa ggccaagacg accgacagcg
     5341 cgtcggcacc caggttcgac agggtgctgg cgccgagcac gccaccgagc aggacgcagc
     5401 agctgagcgc gaacgtcatc gccacggtcc ggacggaccc gacgccggcg gcgatcagca
     5461 cgagcgccac cgcgacgccg aggccgagca tctccatggt caaggcgagc gtgagcagcg
     5521 gcccgaccgc cccgccgtcg gagaaggcga tgccgaggag cagcccgtcg accacgagat
     5581 cgatcccggt gaccaccagc gtcggcgcca tcgaggacgg gccccgcccg
//
LOCUS       DATMFS010000363         5330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_244051, whole genome shotgun sequence.
ACCESSION   DATMFS010000363 DATMFS010000000
VERSION     DATMFS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244051"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VNQ33_06700"
     CDS             complement(<1..1280)
                     /locus_tag="VNQ33_06700"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HWJ97832.1"
                     /translation="MAGNGAQPKRVGVRGQRLLADPALNKGTAFTREERDAFGLHGLL
                     PTHIETIEQRADRVRSQLDDEPTPLGKHMLLRDVQDYDETLFLRVVLDDIYDLLPVVY
                     TPTVGDACEQFSRIYQHHRGLFLSYPDMDRIEDMLRSVPHSEVAVIVVTDGERILGLG
                     DQGADGLGIPIGKLSLYSACGGIDPSTTLPIVLDVGTNNEARRNDPDYLGWRHERIVG
                     EDYDRFIEAVVQGVMNVYPDVLFQFEDFAEHHAHLLLDRYRDRLCTFNDDIQGTATVA
                     LAAVLSALRSTDRELKDQRVCIVGAGTAGTGIAEQLVAAMVAHGLSEEEARSRFFLID
                     RDGLLFDDVDGLEPFQVPLAQPRSAAEGWTVAEEGKASLVEVIEQAKPSILIGVTGVF
                     GLFTEEVVRAMGAADERPVIFPLSNPTTHAEATAS"
     gene            complement(1377..2438)
                     /locus_tag="VNQ33_06705"
     CDS             complement(1377..2438)
                     /locus_tag="VNQ33_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWJ97833.1"
                     /translation="MSTVVACILFLAVIVYSIFGGADFGAGIWDLLAGGARRGRRPRE
                     VIDRSIGPVWEANHVWLIFVFVVLWTAFPTAYASITLTLFVPLAFAALGIVLRGASFA
                     FRHAVYRTEEQRVYGAFFALGSLLVPFAFGCVAGAIATGRVPAGGKAGDPWSSWVNPT
                     SLLGGVLAVVAVAYLSSVFLVFDAGRIADAEMVEYFRRRAVVAAVVAGVVALAGIAIL
                     HADANDLFDELTARALPLVIVSAVGGIASLVLLLRESHRFARVAAVGAVGGIIAAWGV
                     AQWPYVVPQTMTVDATAAPSGTLAATVVVFVLACTFILPSIGFLYWLDQQDALPEEGG
                     DDGIDLAVAPGTDPGASGR"
     gene            complement(2435..3853)
                     /locus_tag="VNQ33_06710"
     CDS             complement(2435..3853)
                     /locus_tag="VNQ33_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWJ97834.1"
                     /translation="MTSTILAVDPVPWARGQMAFTLAFHIILVPLGVSWAFLTLIANH
                     RAIRHDDSDALLLAQRWSKYMAVTFAVGAVTGTVLTFEFGLLWPRFMGRWGEAFGIPF
                     LVEGLFFFLEAIFVAIYIFGWKRMRPWPHFWTGVPVVLAGIGGSVSVVAANAWMNTPS
                     GFTLDAQGNLDTVDPWGVIFNSSMPLNALHMVIAAYIVGGFLVASVYAVGMLKGRNDH
                     YHRLGFTIAFSVAAIAVPLQMGVGDSLARWVYNEQPVKFAAIELVATTGSDKPEILLG
                     RINDDGEIEGGIPLPGVASILSDPSTGTATVVQGLDSVPAADRPTFSQVNTVHLAWDV
                     MVGTATLLLLVALYYGGYWLFRRRLPQSPWFLRVAACCGVLSVIALEAGWVMAEVGRQ
                     PWIVYHYMKVEDAATGNSYVWVTFLAVVVLYAGLGYAAIRVLRTMSRRARDGLSTDDE
                     GPYGPSGPIAGPIEDPVAGEAP"
     gene            3947..4153
                     /locus_tag="VNQ33_06715"
     CDS             3947..4153
                     /locus_tag="VNQ33_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97835.1"
                     /translation="MGSSRSKARKGHEKPKHLPKVGTPENLEWRHKGERDDAFRVFGG
                     RNGAIVVAVLVALALIGLVVVNLP"
     gene            4379..4696
                     /locus_tag="VNQ33_06720"
     CDS             4379..4696
                     /locus_tag="VNQ33_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ97836.1"
                     /translation="MTIGAPHFNVQIDSGTDPLRVVADGELDAASAAALAGALEGADI
                     DTAGLALDLSGVSFIDSSGLRVIAAQVQRSEAAGTPFAVSAASDAVRRIFEMTGLTSL
                     LDS"
     gene            4787..>5330
                     /locus_tag="VNQ33_06725"
     CDS             4787..>5330
                     /locus_tag="VNQ33_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ97837.1"
                     /translation="MNDSPKTSWVPLEHLSPRDAPHLVVLVEDDPGDTILFEEAIADA
                     DPELRVRCVSRTSQLLPLAETDEVACVVLDLGLPGVTGFEALDEIASALPRVAIVVLT
                     GWGDAKAGTEALARGAQDYLVKGDADGRTIARSIRFAVERKRSQRAATALAEANLRQA
                     EQHRLERALLVDPAVVRPDIS"
BASE COUNT          841 a         1828 c         1958 g          703 t
ORIGIN      
        1 gaagcggtgg cctcggcgtg ggtggtgggg ttcgacaggg ggaagatcac cgggcgctcg
       61 tcggccgcgc ccatggcccg cacgacctcc tcggtgaaga ggccgaacac accggtgacg
      121 ccgatgagga tcgacggctt cgcctgctcg atgacctcga cgaggctggc cttgccctcc
      181 tcggcgacgg tccacccctc ggcagccgag cggggctggg cgagcgggac ctggaacggc
      241 tcgaggccgt cgacgtcgtc gaacagcaag ccgtcgcggt cgatgaggaa gaaccgcgac
      301 cgggcctcct cctcggagag cccgtgggcg accatggccg cgacgagctg ctcggcgatg
      361 ccggtgcccg cggtgccggc gccgacgatg cacacccgct ggtccttgag ctcgcggtcg
      421 gtcgaccgca gggcggagag gacggcggcg agggcgacgg tggcggtgcc ctggatgtcg
      481 tcgttgaagg tgcagagccg gtcgcggtag cggtcgagca gcaggtgggc gtggtgctcg
      541 gcgaagtcct cgaactggaa gagcacgtcc gggtacacgt tcatcacgcc ctggacgacg
      601 gcctcgatga accggtcgta gtcctcgccg acgatgcgct cgtggcgcca cccgaggtag
      661 tcggggtcgt tgcggcgcgc ctcgttgttg gtgccgacgt cgagcacgat cgggagcgtc
      721 gtggacgggt cgatgccgcc gcaggccgag tagagcgaga gcttgccgat ggggatgccg
      781 agcccgtcgg cgccctggtc gccgaggccg aggatgcgct cgccatcggt gacgacgatg
      841 accgcgacct ccgagtgggg cacggagcgc agcatgtcct cgatgcggtc catgtcgggg
      901 tacgagagga acaggccccg gtggtgctgg tagatccggc tgaactgctc gcacgcatcg
      961 ccgacggtgg gcgtgtagac gacgggcagc aggtcgtaga tgtcgtcgag gaccacccgc
     1021 aggaacagcg tctcgtcgta gtcctgcacg tcgcgcagca gcatgtgctt gcccagtggc
     1081 gtcggctcgt cgtcgagctg ggagcgcacg cggtcggcgc gctgctcgat ggtctcgatg
     1141 tgggtcggca gcaggccgtg gaggccgaac gcgtcgcgct cttcccgtgt gaacgccgtg
     1201 cccttgttga gcgccggatc ggccaacagc cgctgccccc gcacaccgac tcgcttcggc
     1261 tgcgccccgt tgcctgccat gacccctcct ggctcacgtc cggatgatcc cgcaagccgc
     1321 atcggcccgc ccggacggcc ggacgatagc agcgcggctg ctggtcagcc tgggtgtcag
     1381 cggccactcg cgccgggatc ggtcccggga gcgacggcga ggtcgatgcc gtcgtcgccg
     1441 ccttcttcgg gcagggcgtc ctgctggtcg agccagtaga ggaacccgat ggacgggagg
     1501 atgaaggtgc aggccagcac gaacaccacg accgtggcgg cgagggtgcc cgacggtgcg
     1561 gcggtggcgt ccaccgtcat ggtctggggg acgacgtagg gccactgcgc cacgccccat
     1621 gcggcgatga tcccgccgac ggccccgacc gccgcgaccc gggcgaagcg atgcgactcg
     1681 cgcaggagca gcacgagcga tgcgatgccg ccgacggcgg acacgatcac gagcggcagc
     1741 gcccgcgcgg tcagctcgtc gaacaggtcg ttggcgtcgg catggaggat ggcgatgccg
     1801 gcaagggcga ccacgccggc gaccaccgcg gcgacgacgg cccggcggcg gaagtactcg
     1861 accatctcgg cgtcggcgat gcgccccgca tcgaacacga ggaacaccga cgacaggtac
     1921 gccacggcga cgacggccag cacgccgccg aggagcgacg tcgggttgac ccagctcgac
     1981 cacggatcgc cggccttccc gccggctggc acccggccgg tggcgatcgc cccggcgacg
     2041 cacccgaagg cgaagggcac gaggagggac ccgagcgcga agaacgcgcc gtacacccgc
     2101 tgctcctcgg tccggtagac cgcgtggcgg aaggcgaagc tggcgccgcg cagcacgatc
     2161 ccgagggcgg cgaacgccag cggcacgaac agcgtcaggg tgatggacgc gtaggccgtg
     2221 gggaacgcgg tccagagcac gacgaacacg aagatcagcc agacgtggtt cgcctcccac
     2281 accggcccga tcgagcggtc gatgacctcg cgcggcctgc gcccccgccg ggcgccgccg
     2341 gcgagcaggt cccagatacc ggccccgaag tcggcgccgc cgaagatcga gtagacgatc
     2401 acggcgagga agaggatgca ggcgacgacg gtgctcatgg cgcctcccct gcgaccgggt
     2461 cctcgatcgg cccggcgatc ggccccgacg gcccgtaggg accctcgtca tcggtcgaca
     2521 acccgtcgcg agcccggcgg ctcatcgtcc gcagcacgcg gatggcggcg tacccgaggc
     2581 ccgcgtagag gacgaccacg gcgaggaagg tcacccacac gtagctgttg cccgtggccg
     2641 cgtcctcgac cttcatgtag tggtagacga tccagggttg gcggccgacc tccgccatga
     2701 cccatccggc ctcgagggcg atgaccgaga gcacaccgca gcaggcggcg acgcgcagga
     2761 accacgggga ttgcgggagc cgtcgccgga agagccagta gccgccgtag tagagggcga
     2821 cgagcaagag cagggttgcg gtgccgacca tgacatccca ggccaggtgg acggtgttga
     2881 cctgcgagaa cgtcggccgg tcggcagcag ggaccgagtc caggccctgg accaccgtgg
     2941 cggtgccggt cgaggggtcc gacaggatcg acgccacgcc gggcaggggg atgccgcctt
     3001 cgatctcacc gtcgtcgttg atgcgcccga ggaggatctc cggcttgtcg ctgccggtgg
     3061 tggcgaccag ctcgatcgcc gcgaacttca cgggctgctc gttgtagacc caccgggcga
     3121 gcgagtcgcc gacgcccatc tgcagcggca cggcgatggc cgccacgctg aaggcgatgg
     3181 tgaacccgag ccggtggtag tggtcgttgc ggcccttgag catcccgacg gcgtagaccg
     3241 acgccaccag gaagccgccc acgatgtagg cggcgatgac catgtggagg gcgttcagcg
     3301 gcatcgagct gttgaagatg acgccccacg gatcgaccgt gtcgaggttg ccctgggcgt
     3361 cgagcgtgaa gcccgacggc gtgttcatcc aggcgttggc ggccaccacc gagacgctgc
     3421 caccgatgcc ggcgaggacc accggcacgc ccgtccagaa gtggggccac ggccgcatcc
     3481 gcttccagcc gaagatgtag atggcgacga agatcgcctc gaggaagaag aacagcccct
     3541 cgacgaggaa cgggatgccg aaggcctcgc cccaccggcc catgaaccgt ggccacaaca
     3601 ggccgaactc gaacgtgagc acggtgccgg tcacggcgcc gacggcgaag gtcacggcca
     3661 tgtacttcga ccagcgctgg gcgagcagca gcgcgtcgct gtcgtcgtgg cggatcgccc
     3721 ggtggttggc gatgagggtg aggaacgccc acgacacacc gagcggcacg aggatgatgt
     3781 ggaaggcgag cgtgaaggcc atctggcccc gggcccacgg caccgggtcc accgccaaga
     3841 tcgtgctggt catcgctcgt ccctcgctgc cgcggcccgc ggcgcggagc ttaccgaggc
     3901 ggcgggtggc tgccggggaa aggggtggcg agtagcgtgc cggcccatgg ggagcagcag
     3961 gagcaaggcg cgcaaggggc acgagaagcc gaagcacctg ccgaaggtgg gcacccccga
     4021 gaacctcgag tggcggcaca agggcgagcg cgacgatgcc ttccgcgtgt tcggcgggag
     4081 gaacggcgcg atcgtcgtcg ccgtgctcgt ggcgctggcg ctgatcgggc tggtcgtcgt
     4141 caacctgccc tgagcggcgg cgacgagagc acccggcctc atccggccgt gacgtccggt
     4201 ccgaggtctt cggccggcca ccgccgcgcc tcgtcgcggt ggcgatgccg ccgcgtccgg
     4261 gcgtcggcgc atcggcgcgg gtgcccggcc ttctgaccgc cgccgcgctc atcgccgtcg
     4321 tccaaccacg ggtgccgacc cctgccccgc atgagcgacc cccggctacg atccggacat
     4381 gaccatcgga gccccgcact tcaacgtgca gatcgattcc ggcaccgacc ccctccgagt
     4441 cgtcgccgat ggcgagctcg acgcggcatc ggccgcggcg ctcgccgggg cgctcgaagg
     4501 ggccgacatc gacacggcgg gcctcgcgct cgatctctcg ggcgtgtcgt tcatcgactc
     4561 gtccggcctg cgggtcatcg ccgcccaggt gcagcgatcc gaggcggccg gcaccccgtt
     4621 cgcggtgtcg gcggccagcg acgcggtgcg gcggatcttc gagatgaccg gtctcaccag
     4681 cctcctcgat tcctgatccc acgagcaggt cgttcgtggg tggatcagaa ttcacccgca
     4741 cagggggcgc atccgctgcc tgcgccgacg aacatgcacg cctctcatga acgattctcc
     4801 gaagacctcg tgggtcccgc tcgagcacct gtcgccccgt gacgccccgc acctcgtcgt
     4861 gctggtggag gacgacccgg gcgacaccat cttgttcgag gaggcgatcg ccgacgccga
     4921 cccggagctg cgggtccgat gcgtgtcccg cacctcgcag ctgctccccc tcgccgagac
     4981 cgacgaggtc gcgtgcgtgg tgctcgacct ggggctcccc ggtgtcaccg gcttcgaggc
     5041 cctcgacgag atcgcgtcgg cgctcccccg cgtcgccatc gtcgtgctca ccggctgggg
     5101 cgacgccaag gccggcaccg aggcactggc gcggggcgcg caggactacc tggtgaaggg
     5161 cgacgccgac ggtcgcacca tcgcccggtc gatccgcttc gctgtggagc ggaagcgttc
     5221 gcagcgggcg gccacggcgc tggccgaggc caacctgcgc caggccgagc agcaccggct
     5281 ggagcgggcg ctgctggtgg acccggcggt ggtccggccc gacatctcct
//
LOCUS       DATMFS010000364         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_58626, whole genome shotgun sequence.
ACCESSION   DATMFS010000364 DATMFS010000000
VERSION     DATMFS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58626"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..298
                     /locus_tag="VNQ33_06730"
     CDS             47..298
                     /locus_tag="VNQ33_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWJ97838.1"
                     /translation="MDEVELDWMREGHCRYYPPAAFFPSDGVGVDAARQICAECPVVA
                     ECLEYALEHRIDHGVWGGCSERQRRRILRARRQEAAATA"
     gene            complement(350..556)
                     /gene="tatA"
                     /locus_tag="VNQ33_06735"
     CDS             complement(350..556)
                     /gene="tatA"
                     /locus_tag="VNQ33_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWJ97839.1"
                     /translation="MSLGPPELLIILIIVLVLFGGAKLPKLAKSLGEAQREFKKGQED
                     GSTNATTTSTPSTPASPNDLDKPA"
     gene            642..1421
                     /locus_tag="VNQ33_06740"
     CDS             642..1421
                     /locus_tag="VNQ33_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97840.1"
                     /translation="MLECVVNISEGRDPSVIDAIAAAAGADLLDVHTDGDHHRSVLTL
                     LGEDAPRAVAREAVGRLDLRRHDGVHPRIGVVDVVPFVPLDGATEADAVAARDAFCAW
                     AGAELALPCFRYGPERSLPDVRRGAFAGLVPDCGPPEPHPSAGAVAVGARPVLVAYNV
                     WLAEPDLDRAKAIAAGLRSPSVRALGLRVGDEVQVSMNLIAPDVTGPGAVVDAIAAHA
                     AIDRCELVGLVPRRILELEDAARWVELDLGPDRTIEARRER"
     gene            complement(1426..1734)
                     /locus_tag="VNQ33_06745"
     CDS             complement(1426..1734)
                     /locus_tag="VNQ33_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWJ97841.1"
                     /translation="MNDTTEPADAKGWQDFANCLGVDPDLFFPERGASTREAKEVCKG
                     CVVREDCLEYALGNGEKFGIWGGLSERERRRIRRQRALARRTALEVEAEADTTKTATA
                     "
     gene            1905..>2509
                     /locus_tag="VNQ33_06750"
     CDS             1905..>2509
                     /locus_tag="VNQ33_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ97842.1"
                     /translation="MTETIDAPWEPAPGSDEGYALFAKERRVLADDGTPIASTFRNAE
                     GPGIPIVFANGWSCSDAYWGKLLPLLEAAGHPCLLPDTRGHGRSGLPRDPGRGARNLS
                     IDDVSMPRIARDLVTAMDDAGVQDALIVGHSMGVQTALEVYRQAPERVLGLVLIAGTA
                     SNPAKTFYGTSLGDLTFPVGAAVVRAFPEVLAPVWATLGPAT"
BASE COUNT          319 a          927 c          888 g          375 t
ORIGIN      
        1 ggcgccaggt cgatgaacga gggctgcggc ccggagggag ccagcgatgg acgaggtcga
       61 gctcgattgg atgcgcgaag ggcactgccg gtactacccg cccgcggcgt tcttcccgag
      121 cgacggcgtc ggcgtcgatg ccgcccggca gatctgcgcc gagtgccccg tcgtggccga
      181 gtgcctcgag tacgccctgg agcaccgcat cgaccacggc gtctggggcg gctgctcgga
      241 gcgccagcgc cgccggatcc tgcgtgcccg ccgccaggag gcggccgcga ccgcctgatc
      301 gtccggcgtc agccgaacgg cgggatggcg atcaggcggc ggaccggggt caggcgggct
      361 tgtcgaggtc gttgggcgac gccggcgtcg atggcgtgga cgtcgtcgtg gcgttcgtgc
      421 tgccgtcttc ctggcccttc ttgaactccc gctgcgcctc acccagcgac ttggcgagct
      481 tgggcagctt ggcgccgccg aacagaacca ggacgatgat gaggatgatg aggagttctg
      541 gcggaccgag gctcatgtgt tcaccgtaac cctttcccta ccgtgccacc acgtcggtgc
      601 ccgaattccc agcggcctca cgggtccgag agactgacgc cgtgctcgag tgcgtcgtga
      661 acatcagcga ggggcgggat ccgtcggtga tcgatgccat cgctgccgcg gccggggccg
      721 acctgctcga cgtgcacacc gacggcgacc accaccgatc ggtgctcacg ctcctcggcg
      781 aggacgcgcc acgagccgtg gctcgcgagg cggtcggccg cctcgacctc cggcgccacg
      841 acggcgtcca ccctcgcatc ggcgtggtcg acgtggtgcc cttcgtcccg ctcgacggcg
      901 ccaccgaggc cgacgccgtc gccgcacgcg atgcgttctg cgcctgggcc ggagccgagc
      961 tggcgctccc ctgcttccgc tacggcccgg agcgctcgct ccccgacgtg cggcggggcg
     1021 cgttcgccgg gctcgtgccc gactgcggcc cgcccgagcc gcacccctcc gccggggccg
     1081 tcgccgtcgg tgcccgcccg gtgctggtgg cctacaacgt gtggctcgcc gaacccgacc
     1141 tcgaccgggc caaggcgatc gccgccggcc tccgcagccc gtcggtgcga gcgctcggcc
     1201 tccgagtcgg cgacgaggtg caggtgtcga tgaacctgat cgcccccgac gtcaccggtc
     1261 ccggggccgt ggtggatgca atcgccgccc acgccgcgat cgaccgctgc gagctggtgg
     1321 ggctcgtgcc tcgccggatc ctggagctcg aggatgccgc tcgctgggtc gagctggacc
     1381 tcggccccga tcgcacgatc gaggcccgcc gggagcggta gccgctcagg cggtggcggt
     1441 cttggtggtg tcggcctcgg cctcgacctc cagcgcggtg cgccgggcga gggcccgctg
     1501 ccggcggatg cggcggcgct cacgctcgct caggccaccc cagatgccga acttctcgcc
     1561 gttgccgagc gcgtactcga ggcagtcctc acgcaccacg cagcccttgc agacctcctt
     1621 cgcctcgcgc gtcgaggcac cccgctcggg gaagaagagg tcggggtcga cgccgaggca
     1681 gttggcgaag tcctgccaac ccttggcatc tgctggttcg gtggtgtcgt tcatgggctg
     1741 acccccagag tcgtcccgct tcggccggcg cccgtacgat cggtcgccgt ggtggtcggc
     1801 accggcgacg gtgcatgcca cgcatggaat tacatcgctg tgatcttcgc cgctggcgga
     1861 cgattgtgca agcagaacct ttgctgaggc cacgagggag ccccatgacc gagaccatcg
     1921 acgcaccgtg ggagcccgcg cccgggtccg acgaggggta cgccctgttc gccaaggagc
     1981 gccgggtcct cgccgatgac gggacgccca tcgcgtccac gttccgcaac gccgaggggc
     2041 caggcatccc gatcgtgttc gccaacggct ggtcgtgcag cgacgcctac tggggcaagc
     2101 tcctgccgct gctcgaggcc gccggccatc cctgcctgct gcccgacacc cgaggccacg
     2161 gacgctccgg gctccctcgc gacccgggcc gcggcgcccg caacctgtcg atcgacgacg
     2221 tctcgatgcc gcgcatcgcc cgcgacctcg tgacggcgat ggacgacgcc ggtgtgcagg
     2281 acgcgctcat cgtcgggcac tcgatgggcg tccagaccgc gctcgaggtg taccggcagg
     2341 cgcccgagcg ggtcctgggc ctggtgctca tcgccggaac ggcgtcgaac ccggccaaga
     2401 ccttctacgg cacgtcgctc ggcgacctca cgttcccggt cggagccgcc gtcgtccggg
     2461 cgttccccga ggtgctcgct cccgtgtggg ccaccctcgg ccccgccac
//
LOCUS       DATMFS010000365         5387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_58649, whole genome shotgun sequence.
ACCESSION   DATMFS010000365 DATMFS010000000
VERSION     DATMFS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58649"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..449
                     /locus_tag="VNQ33_06755"
     CDS             84..449
                     /locus_tag="VNQ33_06755"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWJ97843.1"
                     /translation="MIGIGVDLIEVDRMRTVLARTPTMAEKLFTPGERAYAEAANDPT
                     ERYAARFAAKEAVMKALGVGLGAVDWHDIEVVRTDRGAPVLAVTGRAQALAEAAGVTR
                     WLLTMTHTTTMAEAVAVAL"
     gene            complement(649..1044)
                     /locus_tag="VNQ33_06760"
     CDS             complement(649..1044)
                     /locus_tag="VNQ33_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HWJ97844.1"
                     /translation="MSIDPADLPDEALAFLAEYHLASLTTARPDGSPHVVAVGFSYSP
                     ADQTARIITWAASKKARNVAAAPGGRAVVCQIDGGRWLSLEGTATVTDDPARVAEAVD
                     GYAARYRQPGERDDRVAIEIKIDRILGRA"
     gene            complement(1370..2059)
                     /locus_tag="VNQ33_06765"
     CDS             complement(1370..2059)
                     /locus_tag="VNQ33_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ97845.1"
                     /translation="MLHRWSVPALVAGVLALAASTGLTGCVPTPKAGTIDVVGDSLAF
                     QANWARPAEFLVGSPRGADVALDFRPGIGFPDVMAAQRTRVAAARPAILVVALGTNDA
                     GPLDNGWNAADDKELRALMALPSPTTCVVLVLPGAADKPAVTATYRTDLTAARAAMRK
                     AAADRKAKGQPTVVADWGAVALARPELTVADGIHLADDAPSAPGRIVNEAAANAWTKV
                     LWSGVAACPKR"
     gene            2140..>5387
                     /locus_tag="VNQ33_06770"
     CDS             2140..>5387
                     /locus_tag="VNQ33_06770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ97846.1"
                     /translation="MTCSACGAPASDDARFCASCGRPLRSVEDERRVVTVVFADLVGF
                     TSLSERLDPERVKNLVDRCFDRMADDITSFGGQVDKIVGDALVALFGATTAHEDDPER
                     AVRAALRMQASLCEEAEAIGQELQMRVGINTGEVLVGAMRAAGSVTAMGDVVNTASRL
                     QTNAKPGEVLVGPATYAATHHTIAYEQRGLLDAKGRDELVETWAAVAPTLPPGYRARR
                     VDVPLVGRGSELGLLTTVIDSSIRNDRAMLALLVADVGMGKSRLADEVAGSAATVHGA
                     IVREGRCVPYGEANVWWPVADALRPALEVTDGASTDDARTAVRLAVADVMERRTTDPE
                     VQRTAEGLLTLLGYEPPADADPVTIRQEAGRALGAYAAASARHRPLVLQISDLHWADS
                     AVLSLIDDVFSAIHHCPVVVLATARPALLDQWTPRHGRHNALTLHLDPLGREATGELL
                     EHLVGAPVPDAVAAALFERSGGNPFFLEELVSLLDGEAVAEPGRVAALPDTLRGLVAA
                     RLDDLTPSQRGVLQDASVIGQRGPMLGLREMGRQLRSGVDIDAAVADLVADEIMEIDG
                     ELWSFRSDLIREVAYQTITKAERAKCHLGIASFIEQKAAGVDPRPPWVVDQLAHHYGA
                     AVALASELGPDARTDAFPADLASRARRWVTEAADRACRDQALPASIRLYTQALDLLGD
                     IDAQTSSPGDIAEAIRIHLARSTAAAEGWELVLGRRDAERACALADAAGDDAAAARAL
                     IARGAIEQKEGDVDAAVATMSDAADRFRGLGDLGGLGEALRRRGMVEIFGNRIHDAEA
                     SAEAALAAFEEVGDRAGIAWASQNLAWTAFNSGRPDEADRRLQKAIELFSDLVDTQGL
                     AWSLGLLAWVRFQQGRVAEAQALGEQVLDEARARSDPWATAMMLMLVASIRLWAGRTD
                     EAVSMSEQALRTFTGLGDRFGGVQAGAVLGRSLVMAGRVEEGFALLVRVASPDALGAA
                     EGDGFPARYARLAAALQIGEPHRGASVLRDMEVIASAGLGGDDAAAVLAAAALMEGDV
                     AQAEAYVASARTHDVDPNVMAVHALAAAASGSGQAGGLADRIDAATGATYLDR"
BASE COUNT          708 a         1934 c         2014 g          731 t
ORIGIN      
        1 gtcgaatcga cgtcgattcg acgcgccggt gactcggtgg gggcgtggtc ggtgatgcgg
       61 ctgatcggtg agcgggcgcg ggtgtgatcg gcatcggcgt cgacctcatc gaggtcgacc
      121 gcatgcgcac ggtgctggcc cgcaccccga cgatggccga gaagctgttc acgccgggag
      181 agcgtgccta cgcggaggcc gccaacgatc ccaccgagcg ctacgccgcc cgcttcgccg
      241 ccaaggaggc ggtcatgaag gcgctgggcg tggggctcgg ggccgtcgac tggcacgaca
      301 tcgaggtcgt ccgcaccgat cgaggcgctc cggtgctggc ggtcaccggg cgggcccagg
      361 ccctggccga ggccgccggc gtcacccgct ggctgctcac gatgacccac accacgacca
      421 tggccgaagc cgtcgccgtc gccctctgac cctcgaaccg cgctccctgc gccgttctcg
      481 tcgggcgggg ccggcccagg accgccggcc ctcacggttc gaacgatgcg cgcacggcac
      541 gcatcacacg tcgagccggg tggtcggacg aacggacgtt cgatgcgaac ggttggttgc
      601 ggtgcagcca ccggcgttga acgttcggga ttgtggcccc gggaggcgtc aagctcggcc
      661 gaggatgcga tcgattttga tctcgatggc gacgcggtcg tcgcgctcgc cgggctggcg
      721 gtagcgggcg gcgtagccgt cgacggcctc ggcgacgcgg gcggggtcgt cggtgaccgt
      781 ggcggtcccc tcgagcgaga gccagcgccc gccgtcgatc tggcacacca ccgcccggcc
      841 acccggcgcg gccgcgacgt tgcgggcctt cttgctggcc gcccaggtga tgatgcgggc
      901 agtctggtcg gcgggggagt agctgaaccc gacggcgacg acgtgcggcg agccgtcggg
      961 tcgggccgtg gtcagcgagg cgaggtggta ctcggcgagg aaggccagcg cctcgtcggg
     1021 aaggtccgcg ggatcgatcg acatccgacc atcctgccgc accccctccg cgaacctcga
     1081 tgcccccgcc gaacccgtgc gcgtcgaatc gacgtcgatt cgacgcgcac ggggggcgtg
     1141 acggcaccac gggctggggg ctggcgctcg accgcgtggg cgccgccgct tcgatgtcgt
     1201 cgccgcccgt ggccgattcg acggattcga cgtgacggcg tcgacagatt cgacggatcc
     1261 gatgaggtgg ctcgacccca ccgattcgac cgattcgacg gattcgacgt gacggtgtcg
     1321 gccgattcga cgtgaccgga tgcgacgtcg acgatccgcc gtgcgctggt cagcgcttgg
     1381 ggcaggcggc gacgccggac cagaggacct tggtccaggc gttggcggca gcctcgttca
     1441 cgatgcggcc gggggcgctg ggggcgtcgt cggcgaggtg gatgccgtcg gcgacggtga
     1501 gctcgggacg ggcgagggcg accgcgcccc agtcggccac gaccgtgggc tgacccttgg
     1561 ccttgcggtc cgccgcggcc ttgcgcatgg cagcgcgggc ggcggtcaga tcggtgcggt
     1621 aggtggcggt gaccgccggc ttgtcggcgg cgcccgggag cacgagcacg acgcacgtgg
     1681 tgggcgaggg cagcgccatc agggcccgca gctccttgtc gtccgccgcg ttccagccgt
     1741 tgtcgagagg cccggcgtcg ttcgtgccga gcgcgacgac gaggatcgcc ggccgggcgg
     1801 ccgcgacccg cgtgcgctgg gcggccatca cgtcggggaa gccgatgccc ggacggaagt
     1861 ccaaggccac gtcggcgccc cggggtgagc cgaccaggaa ctcggcgggt cgggcccagt
     1921 tcgcctggaa ggcgagggag tcgcccacca cgtcgatcgt gccggccttg ggcgtgggca
     1981 cgcagccggt caatccggtg gaggccgcga gcgccagtac cccggcgacc agggcgggga
     2041 cggaccaccg gtgcagcacg ccgccatgtt cgcgggtggc ggccggccgt gcgcaaacga
     2101 gccgcgctaa ctgtggcaag cagccgtagg atgccgacga tgacctgttc ggcatgcggg
     2161 gctccggcat cggatgacgc tcgcttctgc gcgtcgtgcg gccggccgct gcgcagcgtc
     2221 gaggacgagc gccgggtcgt cacggtggtg ttcgccgacc tggtcgggtt cacctcgctg
     2281 tcggagcgac tcgatccgga gcgggtgaag aacctggtgg accgctgctt cgaccgcatg
     2341 gccgacgaca tcaccagctt cggaggccag gtcgacaaga tcgtgggcga tgccctggtg
     2401 gccctgttcg gcgccaccac cgcacacgag gacgaccccg agcgggcggt gcgagcggcc
     2461 ctgcgcatgc aggcatcgct gtgcgaagag gccgaggcca tcggccagga gctgcagatg
     2521 cgcgtcggca tcaacaccgg cgaggtgctg gtgggcgcca tgcgagccgc gggctcggtg
     2581 acggcgatgg gcgatgtggt caacaccgcc agccgcctcc agaccaacgc caagcccggt
     2641 gaggtcctcg tgggcccggc cacctacgcc gccacgcacc acaccatcgc ctacgagcag
     2701 cgcggcctgc tcgatgccaa gggccgcgac gagctggtgg agacctgggc agccgtcgcg
     2761 ccgacgctcc cgcccggcta ccgggcccgc cgcgtcgacg tgccgctcgt cggccgcggc
     2821 tcggagctcg gcttgttgac caccgtcatc gacagctcga tccgcaacga ccgggcgatg
     2881 ctcgcgctct tggtggccga cgtcggcatg ggcaagagcc gcttggctga cgaggtcgcc
     2941 ggttcggctg ccaccgtcca cggcgccatc gtgcgggagg gacgctgtgt cccctacggc
     3001 gaggcgaacg tgtggtggcc ggtggccgac gccctccggc cggccctcga ggtcaccgac
     3061 ggcgcctcga ccgacgatgc ccgcaccgcg gtgcgcctcg ccgtcgccga cgtgatggag
     3121 cgccggacca ccgaccccga ggtccagcgc acggctgagg ggctcctcac gctgctcggc
     3181 tacgagcctc cggcggacgc cgatccggtc accatccgcc aggaggcggg gcgtgcgctc
     3241 ggggcctacg ccgcggcgtc ggctcgacac cgcccgctgg tgctgcagat ctcggacctg
     3301 cactgggccg acagcgccgt gctgtcgctc atcgacgacg tcttcagcgc gatccatcac
     3361 tgcccggtcg tggtgctcgc caccgcccgc ccggcgctgc tcgatcagtg gacaccgcgc
     3421 catggccgtc acaacgccct caccctccac ctggaccccc tgggccgcga ggccaccggt
     3481 gagctgctcg agcacctcgt gggtgcgccg gtgcccgatg cggtggccgc ggccctgttc
     3541 gagcgcagcg gcggcaaccc cttcttcctc gaggagctgg tgagcctgct cgatggcgag
     3601 gcggtggccg aacccggccg cgtcgccgct ttgcccgaca ccctccgagg tctggtggcg
     3661 gcccgcctcg acgacctcac gccctcgcag cgcggggtcc tccaggacgc cagcgtcatc
     3721 ggccagcgcg gcccgatgct cggcctccgg gagatggggc ggcagctgcg gtccggcgtc
     3781 gacatcgacg cggcggtggc ggacctcgtg gccgacgaga tcatggagat cgacggcgag
     3841 ctgtggtcgt tccgctccga cctcatccgc gaggtggcct accagaccat caccaaggcc
     3901 gagcgcgcca agtgccacct cggcatcgcc tcgttcatcg agcagaaggc agcgggcgtc
     3961 gacccgcgcc cgccgtgggt ggtggaccag ctcgcccacc actacggggc ggccgtcgcc
     4021 ctggcatccg agctcggccc cgacgcacgg accgatgcgt tcccggccga cctcgccagc
     4081 cgggcgcgcc ggtgggtcac cgaggccgcc gatcgcgcct gtcgggacca ggccctcccg
     4141 gcgtcgatcc ggctgtacac gcaagccctc gacctgctcg gcgacatcga cgcccagacg
     4201 tcctcgccag gggacatcgc cgaggccatc cgcatccacc tggcccggtc gacggccgcc
     4261 gccgaaggat gggagctggt gctgggccgc cgcgacgccg agcgcgcctg cgcgctggcc
     4321 gatgctgccg gcgacgatgc tgcggccgcc cgagccctca tcgcccgcgg ggccatcgag
     4381 cagaaggaag gcgatgtcga cgctgccgtc gccacgatgt ccgacgccgc cgaccgcttc
     4441 cgcgggctcg gtgacctggg cgggctgggt gaagcgctcc gtcgtcgggg catggtcgag
     4501 atcttcggca accgcatcca cgacgccgag gcgtcggccg aagccgctct cgccgcgttc
     4561 gaggaggtcg gcgaccgcgc cggcatcgcc tgggcgtcgc agaacctggc gtggacggcg
     4621 ttcaacagcg gccgccccga cgaagccgac cgtcgtctgc agaaggccat cgagctgttc
     4681 agcgatctgg tcgacacgca ggggctggcc tggagcctcg gcctgctggc gtgggtccgg
     4741 ttccagcagg gccgcgtggc cgaggcgcag gcgctcggcg agcaggtgct cgacgaggcc
     4801 cgagcccgca gcgatccgtg ggccacggcg atgatgctga tgctggtggc gtcgatccgc
     4861 ctgtgggccg gccgcaccga cgaagccgtc tcgatgtccg agcaggccct gcggaccttc
     4921 acgggcctgg gcgaccgctt cggcggcgtg caggccggcg ccgtgctcgg ccgctcgctc
     4981 gtcatggcgg gccgggtcga ggagggcttc gcgctcttgg tgagggtcgc cagccccgat
     5041 gctctgggcg cggccgaggg cgacggcttc cccgcccgct acgcccggct cgccgccgcg
     5101 ctgcagatcg gcgagcccca ccggggcgca tcggtgctcc gcgacatgga ggtgatcgcc
     5161 agcgccggcc tgggcggcga tgacgccgcg gcggtcttgg ccgccgccgc gctgatggaa
     5221 ggtgacgtcg cccaggcgga ggcctacgtc gccagcgccc gcacgcacga cgtcgacccc
     5281 aacgtgatgg cggtgcacgc cctcgcggcg gccgcgtcgg gctccgggca ggctggtggg
     5341 ctcgccgacc gcatcgacgc cgccaccggc gcgacctacc tcgaccg
//
LOCUS       DATMFS010000366         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_315670, whole genome shotgun sequence.
ACCESSION   DATMFS010000366 DATMFS010000000
VERSION     DATMFS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315670"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VNQ33_06775"
     CDS             <1..629
                     /locus_tag="VNQ33_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ97847.1"
                     /translation="GLEYVNFLVPGFLVTVILWTGMSAAAGVAEESSTGVHDRLRSLP
                     IPRAAVMAGRSIADGALTTWGIFVTAIIGLLVGWRPDGGAARIVVGFALMLIAGYAFS
                     WIFIFIGLVAGNAQAAQGLSMLVIPFSFISSANVPVSSMPGWMQPFAANQPISVIINA
                     VRGSMIGGVDQLGVGHTLGYWTVLSLVWCAGILIVFGFLSVRRFSKMK"
     gene            complement(653..1705)
                     /locus_tag="VNQ33_06780"
     CDS             complement(653..1705)
                     /locus_tag="VNQ33_06780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWJ97848.1"
                     /translation="MADDDLKLGLVLGYWFAGPPAGVPATLAAAEDLGFDSIWSAEAY
                     GSDCFTPLAWYGAATSRAKLGTAVCQMSARTPVATAMSALTLDHLSGGRFQLGLGASG
                     PQVVEGWYGQPYPKPLARTREYVEIIRRVLAREEPLDFQGEHYQLPNRGPGTMELGKP
                     LKSITHPLRADIPILLGAEGPKNVAMAAEIADGWLPIFLSPELDKHYRDCLAEGFARP
                     GARRTLETFEIPNMVNIVVNDDVEAAADAVRMFLGFYIGGMGAKEVNFHANLFARMGY
                     GDEVEEIQDLFLAGRKDEAIKRVPLALVEDVAMVGPLDKVREELDEKWRKTCMTTLLV
                     NGGPDQLRLVADLVNG"
     gene            1820..>3240
                     /locus_tag="VNQ33_06785"
     CDS             1820..>3240
                     /locus_tag="VNQ33_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97849.1"
                     /translation="MNRPTTRWRTCRGALALALAGAGVAAVLAIATPPVEASTTRTAV
                     TRLEPVTWVDGQGRQYVLADGAFAFGSTPPSPYQLFVARDRADGEPDATWGAGGSNRR
                     LLSPAPPAEGATLDRIAAATSDGTITLVWDAPGCASTSTCDRWFERIDATGATVAAPV
                     LVDDLAPPFQALGDGSVLTGTGTGPLGWYGPDGADRGAPAVEAATVKSAAVDADGRLL
                     LATAEGTVRRWPIAGPSDLTLDSSCAGADGIAIGSSVTAGDGFAVACSSTDDPVSVTR
                     YGDGGSVAWTASDTAAPESTSHLRAPTDLAVTTDGTVWVGGGGVLPISAAFPISATQV
                     ASFTQAGTVTRHYERPTIRPGSYDQGVTGVSELRPVDATHVAVADIQRCCFTIEGMMP
                     ESSVTGQVLPLRPAPPACNRPAATIAAATASTLDVHFTTCAEGEPNRAPTGYRVEVAS
                     TAGTVTNDVPAPAASTSASTTVAG"
BASE COUNT          435 a         1208 c         1132 g          465 t
ORIGIN      
        1 ccgggctcga gtacgtgaac ttcctcgtac cgggcttctt ggtcaccgtc atcctctgga
       61 ccggcatgag cgccgcggcc ggcgtggccg aggagtcatc caccggcgtg cacgaccgcc
      121 tgcgctcgct gcccatcccc cgggccgcgg tcatggccgg ccggtccatc gctgatggcg
      181 ccctcacgac ctggggcatc ttcgtgaccg cgatcatcgg cctgctggtc gggtggcggc
      241 ccgacggcgg cgccgccagg atcgtcgtcg gcttcgcgct gatgctgatc gccgggtacg
      301 ccttcagctg gatcttcatc ttcatcggcc tggtggccgg caacgcccag gcggcgcagg
      361 gcctgtcgat gctggtgatc ccgttctcgt tcatctccag cgccaacgtg ccggtgagct
      421 cgatgccggg gtggatgcag cccttcgccg ccaaccagcc catcagcgtg atcatcaacg
      481 ccgtccgcgg ctccatgatc ggcggggtcg accagctcgg cgtgggccac accctcgggt
      541 actggacggt gctgtcgctg gtctggtgcg ccgggatctt gatcgtgttc gggttcctca
      601 gcgtccgccg cttctccaag atgaagtagc cggccctgcg ggtggacggc ggtcagccgt
      661 tgaccaggtc tgccaccagg cggagctgat cgggaccgcc gttcaccagc agcgtcgtca
      721 tgcaggtctt gcgccacttc tcatcgagct cctcgcggac cttgtcgagc gggccgacca
      781 tggcgacgtc ttccaccagg gcgagcggga cccgcttgat ggcctcgtct ttgcggccgg
      841 ccaggaacag gtcctggatc tcctccacct cgtcgccgta gcccatgcgg gcgaacaggt
      901 tggcgtggaa gttgacctcc ttggccccca tcccgccgat gtagaagccg aggaacatcc
      961 ggaccgcatc agccgcagcc tccacgtcgt cgttcaccac gatgttcacc atgttcggga
     1021 tctcgaaggt ctcgagcgtg cgacgcgcac cgggccgggc gaagccctcg gccaggcagt
     1081 cgcggtagtg cttgtcgagc tccggcgaca ggaagatcgg cagccacccg tcggcgatct
     1141 ccgccgccat ggcgacgttc ttggggccct cggcaccgag gaggatcggg atgtcggcgc
     1201 gcagcgggtg ggtgatgctc ttgagcggct tgcccagctc catcgtgccc ggaccccggt
     1261 tgggcagctg gtagtgctcg ccctggaagt ccagcggctc ctcgcgggcc agcacccggc
     1321 ggatgatctc cacgtactcc cgcgtgcggg ccagcggctt cgggtacggc tggccgtacc
     1381 agccctccac cacctgaggc ccgctggcgc cgaggccgag ctggaagcgc ccgccggaga
     1441 ggtggtcgag ggtgagcgcg ctcatggccg tggccacggg cgtgcgggcc gacatctggc
     1501 agaccgcggt gccgagcttg gcccgcgagg tggcggcgcc gtaccaggcg agcggcgtga
     1561 agcagtcgga tccgtacgct tcggccgacc agatcgagtc gaacccgagg tcctccgcgg
     1621 cggccagcgt cgcgggcacc ccggcaggcg ggccggcgaa ccagtatcca aggaccaaac
     1681 cgagcttgag atcgtcatca gccatgcggg gcaccgtaat ccccggaacg aaagcggttc
     1741 ctcgctggtc gggggatcag aagccacgtc ccggcggccc ggttcgggtc ggcaccgcgg
     1801 gcggacaagg gagatcgaca tgaaccgacc gacgacccgg tggcggacct gccgcggcgc
     1861 cctggcgctg gccctcgccg gagcaggggt tgccgccgtc ctcgccatcg ccacgccgcc
     1921 cgtcgaggcg agcacgaccc gcaccgccgt gacgcgcctc gagccggtga cctgggtcga
     1981 cggccaaggc cgccagtacg tcctcgccga cggcgcgttc gccttcggct ccacgccgcc
     2041 gagcccctac cagctgttcg tggcgcgcga tcgagccgac ggcgaacccg atgccacgtg
     2101 gggtgccggc ggttcgaacc ggcgcctcct ctcccccgct cctccggccg agggcgccac
     2161 gctcgaccgg atcgcggcgg cgacgtccga cggcacgatc accctcgtct gggacgcgcc
     2221 cggctgcgcg agcaccagca cctgcgaccg ctggttcgag cgcatcgacg ccaccggcgc
     2281 gaccgtcgcc gccccggtgc tcgtcgacga cctggcgccg ccgttccagg cgctcgggga
     2341 cgggtccgtg ctgaccggta ccggcaccgg cccgctcggg tggtacgggc cggacggcgc
     2401 ggaccggggc gcgcccgccg tcgaggccgc cacggtgaag tcggcagcgg tcgacgccga
     2461 cggccgcctg ctgctggcca ccgccgaggg cacggtccgg cgctggccca tcgcgggtcc
     2521 gtccgacctc accctcgaca gcagctgtgc gggcgccgac ggcatcgcca tcggctcgtc
     2581 ggtcacggcc ggcgacgggt tcgcggtggc gtgcagctcg accgacgacc cggtgtcggt
     2641 cacccgctac ggcgacggcg gctcggtggc ctggaccgcg tcggacaccg ccgcgcccga
     2701 gagcacctcg cacctccggg cgcccaccga cctggcggtc acgaccgacg gcaccgtctg
     2761 ggtcggcggc ggcggggtgc tcccgatctc ggcggcgttc ccgatcagcg ccacgcaggt
     2821 ggcgtcgttc acccaggccg ggacggtcac ccgccactac gagcggccga cgatccggcc
     2881 cgggagctac gaccagggcg tcaccggcgt gtcggagctc cggccggtgg acgccacgca
     2941 cgtcgcggtc gccgacatcc agcgctgctg cttcacgatc gaaggcatga tgccggagag
     3001 cagcgtgacc ggccaggtcc ttcccctgcg accggcgcct cccgcgtgca accgccccgc
     3061 ggcgaccatc gcagccgcca ccgcctccac cctcgacgtg cacttcacga cctgcgcgga
     3121 gggcgagccg aaccgtgccc ccaccggcta ccgggtggag gtcgcatcga ccgcgggaac
     3181 cgtcacgaac gacgtcccgg cgccggcggc gtcgaccagc gccagcacga ccgtcgccgg
//
LOCUS       DATMFS010000367         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_129166, whole genome shotgun sequence.
ACCESSION   DATMFS010000367 DATMFS010000000
VERSION     DATMFS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129166"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VNQ33_06790"
     CDS             complement(<1..480)
                     /locus_tag="VNQ33_06790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ97850.1"
                     /translation="MTSNCLQVLGPSAGGIRRHVATLAEGLEARGRAVTVAGPAGVMD
                     GLWPDAAAVPAALGASAPSAVRRLRPLVRQADVVHAHGLTAGWLAWAAGAGPKLVVTV
                     HNLVLDEAAGASTAVLRRLEGWLPGRACETIVISAPMQARFDATGTDPHVHLIPPVAP
                     "
     gene            complement(477..2105)
                     /locus_tag="VNQ33_06795"
     CDS             complement(477..2105)
                     /locus_tag="VNQ33_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HWJ97851.1"
                     /translation="MTAEAGDRVAVATPAGLARSTVTMSVLTAVSRATGLVRIILVGA
                     VVGTTYLGNTYETTNTIPNIVFELMAAGTFQAVLIPALVRYESKDDQEQAEHVASSVF
                     GIALVALMGVAAVGMLMSPWIADLLFSGSSEAVRAEQVRLGTIFLLIFLPQVGMYAVG
                     MVATAVLNAQNRFAAPAIAPAVNNVIVCAAYLLFWLSREGGPPVLELSNLQIALLAGG
                     TTLGVVGFCAVPLLAVRRTSFRLRIRFDRRHPAVRQVLRMGVWAAGFLATTQMLILVE
                     LLLSNKVEGGVVALQIGWTFFLLPYALFAQPILTALFPTMSRQATRRDHTGFAHSVES
                     GTEMICLLVIPCAIAFVAVAPAMCRAILFGAIDAAGAADVARVVVAFAPGVVGYGLLL
                     FYARVLYAREDARTPTIVNLAAGIVGAVLMLITSSLPDREWQVPLLAAIHAFAYSLAA
                     IALALIIRGRLPEGNRPALLRRLRPQLLAIVPVGVAGTLVGRQVPVHSRAEALVLGGI
                     VGVGIIVAFALVTAALGGPRPTALLGALKRGGRR"
     gene            complement(2102..>2381)
                     /locus_tag="VNQ33_06800"
     CDS             complement(2102..>2381)
                     /locus_tag="VNQ33_06800"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HWJ97852.1"
                     /translation="VLLNILPSTGEALPATVISATQTASGDEPVVPTVVARIRANRPL
                     LERYSTVDNTETFAGLSAMLFTLRDMGSVAPGQYGQAAGATAILPPSP"
BASE COUNT          388 a          800 c          920 g          273 t
ORIGIN      
        1 cggggccacc ggagggatca ggtggacgtg cggatcggtt cccgtggcat cgaagcgggc
       61 ctgcatgggg gccgagatga cgatggtctc gcaggcccgc cccggcagcc atccttccaa
      121 ccggcggagc acggccgtcg aggcgccggc cgcctcgtcg agcacgaggt tgtgcacggt
      181 gacgacgagc ttcggtccgg cgcccgcggc ccacgccagc cagccggccg tgagcccgtg
      241 cgcgtgcacg acgtcggcct ggcggaccag cggacggagc cgacggaccg cggagggggc
      301 cgatgccccc agcgccgcgg ggacggccgc cgcgtccggc cagaggccgt ccatgacgcc
      361 cgcgggcccg gctaccgtga cggcacggcc gcgtgcctcg agcccctcgg cgagcgtggc
      421 gacgtggcgg cggatgcctc cggcggacgg gccgagcacc tggaggcagt tcgaggtcat
      481 cgccgccccc cgcgcttgag ggcgccgagc agggcggtgg gccggggacc ccccagcgcc
      541 gcggtcacca gcgcgaaggc gacgatgatg ccgaccccca cgatgcctcc gagcaccagc
      601 gcctcggctc gcgagtgcac cggaacctgg cggccgacga gcgtgccggc gacgccgacg
      661 ggaacgatgg cgagcagctg cggccgcagc cggcgcagga gcgcaggacg gttcccctcg
      721 gggagccgtc cccggatgat cagggcgagg gcgatggcgg cgaggctgta ggcgaaggcg
      781 tggatggcgg cgaggagcgg cacctgccac tcccggtcgg gcagcgacga ggtgatcagc
      841 atcaagaccg ctcccacgat gccggcggcc aggttcacga tggtgggcgt gcgggcgtcc
      901 tcacgggcgt agagcacccg tgcgtagaac agcagcaggc cgtagccgac cacaccgggc
      961 gcgaacgcca ccacgacgcg cgccacgtcg gcggcaccgg cggcgtcgat cgcgccgaag
     1021 aggatcgctc ggcacatggc tggagcgacg gcgacgaatg cgatggcgca ggggatcacg
     1081 aggaggcaga tcatctcggt gccgctctcg accgagtggg cgaaccccgt gtggtcgcgc
     1141 cgggtcgctt ggcgggacat cgtcgggaag agggcggtga ggatcggctg ggcgaacagc
     1201 gcgtagggca gcaagaagaa cgtccaaccg atctggaggg ccaccacccc gccctcgacc
     1261 ttgttcgaca ggaggagctc caccaggatc agcatctggg tggtggccaa gaagcccgca
     1321 gcccagaccc ccatgcgcag gacctggcgc accgcggggt ggcgccggtc gaagcggatg
     1381 cggagccgga acgaggtgcg tcgcacggcg aggagcggca ccgcgcagaa gccgacgacg
     1441 ccgagcgtcg tgccccccgc cagcagcgcg atctggaggt tggagagctc gagcaccggg
     1501 ggcccgccct cgcgcgagag ccagaacaag aggtacgccg cgcacacgat gacgttgttg
     1561 acggccgggg cgatggccgg cgcggcgaag cggttctgcg cgttcagcac cgccgtggcc
     1621 accatcccga cggcgtacat gcccacctgc ggcaggaaga tgagcaggaa gatggtgccc
     1681 agccgcacct gctcggcgcg cacggcctcc gacgaaccgc tgaacagcag gtcggcgatc
     1741 cacggcgaca tgagcatgcc caccgcggcg acacccatca gcgccaccag cgcgatgccg
     1801 aacaccgacg aggccacgtg ctcggcctgc tcctggtcgt ctttggactc gtagcggacg
     1861 agggccggga tcagcacggc ctggaacgtg cctgccgcca tgagctcgaa cacgatgttg
     1921 gggatcgtgt tggtggtctc gtaggtgttg ccgaggtagg tcgtgccgac gacggcgccg
     1981 acgaggatga tgcggacgag cccggtcgca cgggagacgg ccgtgagcac ggacatggtg
     2041 accgtgctgc gggcgagccc cgctggtgtg gcgacggcga cccggtcgcc ggcctctgcg
     2101 gtcatgggga cggcggaagg atggccgtgg ctcccgcagc ctgtccgtac tggccaggcg
     2161 cgaccgagcc catgtcgcgg agcgtgaaca gcatcgccga caagccggcg aaggtctcgg
     2221 tgttgtcgac ggtgctgtac cgctccagca gcggccggtt ggcccggatc cgggcgacga
     2281 cggtcggcac caccggctcg tcgcctgagg cggtctgggt ggcgctgatc acggtggcgg
     2341 ggagcgcctc accggtggac gggaggatgt tcaacagcac g
//
LOCUS       DATMFS010000368         4842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_107275, whole genome shotgun sequence.
ACCESSION   DATMFS010000368 DATMFS010000000
VERSION     DATMFS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107275"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VNQ33_06805"
     CDS             complement(<1..467)
                     /locus_tag="VNQ33_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97853.1"
                     /translation="MSAEPSRRLTIGLLGLILLAGTAMRFVGVGWDGGNHLNPDERFL
                     TLVNADISAPDGPAGYFDTGRSALNPANHGDDVVYGTAPVFAVKGVSAWLHDGAETGA
                     QPARAVVGTLDRLGVPLLDDEGNPTFDAGYESLYVGRAVSALVDVFTIALVFEL"
     gene            complement(464..2527)
                     /locus_tag="VNQ33_06810"
     CDS             complement(464..2527)
                     /locus_tag="VNQ33_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ97854.1"
                     /translation="MPRVLTVLTFYDPHWTGLTVFARRIAEGLAADGCDVTVVCSHHD
                     PQTSRRDERNGVAIVRVPTAGRLSRTLLMPALPLVLHRLVPHHDVVHLHSPMAEAELV
                     AATCRRHRVPLVVTHQGDVVMPDTPAEQLVQRLMRATLRRTFQQAARVVTHNDDYAAH
                     ASPAVAGTRALAIDPPVVFDPPEPGATDELRSELGLGDRPVVAFAGRWVEEKGFDVLL
                     RAAPKVLAARPDVAFVFAGETSVTYEGFRERCQPLADPLGPALIELGLILDQRRLAAF
                     YAMADVFVLPSRSDCHASVQIEAMLCGTPVVASDIVGARSIVGRTGAGLLVPPEDPDA
                     LAAGITEVLLDPGRFAGALAAVPSLFPPADGILRYRALMDEVIAEHTAGLPLRPEPAV
                     PSAGPGGTVARLVATDLDPAYRRRTRWLLERVARTPGARVLDAGSGLGTALHLLGQAL
                     PDAVAIGTDVDLQRHVDGRRSGVDAPVTAADLAALPHADEVFDVVLCSEVLEHVADDA
                     AALAELHRVLRPGGRLLVSVPHADFPAAWDPPNRLLAWIGLPLIRRGILAGAWTDHQR
                     LYRPDELLRSLRAAGFVVDEVEEQTHRCLPFTHLLVYGVALRISRAGLVPLTWERDLG
                     RYRSAGPAAAAARADERPSLGLRLAQVLRRRVDRDDLANGWVPPGTRRFVSIVAAAHR
                     PDGSR"
     gene            2569..4476
                     /locus_tag="VNQ33_06815"
     CDS             2569..4476
                     /locus_tag="VNQ33_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97855.1"
                     /translation="MSGLTVLFLLVCAAAGFGITGLTRLPIRFEERVAVAGPLGALAV
                     GLGGWGWSVAIGFDRTSLGLAVVTATLVSLPGWTRIAPGLGDEARGAWRRVRLPWRDG
                     ESLRPLVLLLVVAWPVTIRIFALAWMASGQGGLDVGHLATYADGSAHLAYAGRFVSGG
                     EVPIASPIAAGEPARYHLLADLFAAQASLLGVSLTSALAVTSGFLALFFPAIAYCCGV
                     RLVANRWAALVGVVVFCAGGTLGFVHLFGDIGDHGWGILWQLPRDYSRDPASLLWMDN
                     PSLSYLHAQRNGLFGLPLGLVAVTLLRCGVEERRAAAFTAAGVLVGAVPLANGFAFVV
                     PMAIASAWAVMDRHRPWWRFFLPALVLGLPAAWWLQPAESSVRWFPGWMADDGVGGWL
                     VFWARNAGPFLVLLVVACAWRGTVPRRTVRAFLPLWLLWIVPNLVAFHPWEWNNTKYF
                     AYWQLIGSFLVGAVLVRLVREGIAGRVAAGVVVAVVVVVALTASGVADLSRAMDRSAT
                     RIPWASADALEVAAWLRDSTDPGVVVAAAPAIDEPVVALSGRRFVSGFPGWTFDLGVP
                     DWAARSDDEQAILRGGADGLAAARRRGVDLVVIGPIERGPSFQADEAWWAANASLVHR
                     FGDWSVYAVPR"
     gene            4473..>4842
                     /locus_tag="VNQ33_06820"
     CDS             4473..>4842
                     /locus_tag="VNQ33_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97856.1"
                     /translation="MSVPARAGARAGFVVVAAASAAYAFDQVRHRAFAASTWDITLAW
                     GVGVGCLVGLAAVGRGRAEEGGDRRGLIARGRHWRPTPYEAAGLSILAVASVARLAVL
                     GRFPTVIDGDEGVFSSQAVLA"
BASE COUNT          555 a         1698 c         1906 g          683 t
ORIGIN      
        1 agctcgaaca ccaaggcgat cgtgaagacg tcgaccagcg cggacacggc ccgtcccacg
       61 tacagcgact cgtacccggc gtcgaacgtc gggttcccct cgtcgtcgag gaggggaacc
      121 ccgagccggt cgagcgtccc caccacggcg cgggcgggct gggcccccgt ctcggctccg
      181 tcgtgcagcc acgccgagac gcccttcacg gcgaagaccg gtgcggtccc gtagacgacg
      241 tcgtcgccgt ggttggccgg gttcagggcg gagcggccgg tgtcgaagta cccggccgga
      301 ccgtcggggg ccgagatgtc ggcgttcacg agggtgagga accgctcgtc ggggttgagg
      361 tggttgccgc cgtcccagcc gacgccgacg aaccgcatcg ccgttccggc gaggaggatg
      421 aggccgagca gcccgatggt gagccggcgc gacggctccg cgctcaccgg ctgccatccg
      481 gccggtgcgc ggcagcgacg atcgacacga accgtcgagt cccgggcggc acccatccgt
      541 tggcgagatc gtcacggtcc acccggcgcc ggaggacctg ggcgaggcgc aagccgagcg
      601 agggccgctc gtctgctctg gctgccgccg ccgccggacc cgccgaccgg tagcgcccca
      661 ggtcccgctc ccacgtgagc gggaccagcc cagcccggga gatgcgcagg gccacgccgt
      721 acacgagcag gtgggtgaag ggcaggcagc ggtgggtctg ctcctcgacc tcgtcgacga
      781 cgaagccggc ggcacggagc gaccggagga gctcgtcggg tcggtacagc cgctggtggt
      841 cggtccaggc gccggcgagg atgccgcggc ggatcagcgg gagcccgatc caggcgagca
      901 gccggttcgg cgggtcccag gcggcgggga agtcagcgtg gggcaccgac acgaggagcc
      961 ggccccccgg ccgcagcacg cggtgcagct ccgccagggc ggcggcgtcg tccgcaacgt
     1021 gctcgagcac ctcgctgcag agcaccacgt cgaacacctc gtcggcatgg gggagcgccg
     1081 ccaggtccgc ggcggtgacc ggcgcgtcca cgcccgagcg gcggccgtcg acgtggcgct
     1141 ggagatcgac gtcggtgccg atggcgacgg cgtcgggcag ggcctggccg agcaggtgca
     1201 gcgccgtgcc caggccggag ccggcatcga gcacccgggc accgggcgtg cgggcgaccc
     1261 gctcgagcag ccagcgcgtg cggcgtcggt aggccgggtc cagatcggtc gccaccaggc
     1321 gggccacggt cccgccgggc ccagccgacg gcacggccgg ttcgggccgc agcggcaggc
     1381 cggcggtgtg ctcggcgatc acctcgtcca tgagggcgcg gtaccggagg atcccgtcgg
     1441 ccggggggaa caaggacggc accgcggcca gcgcgccggc gaagcggccc ggatcgagca
     1501 gcacctcggt gatgcccgca gcgagggcgt cggggtcctc gggcggcacg agcaggccgg
     1561 cgcccgtgcg gcccacgatc gaccgagcgc cgacgatgtc cgaggccacg accggcgtcc
     1621 cgcacagcat cgcctcgatc tggacgctgg cgtggcagtc ggaccgcgag ggcaggacga
     1681 acacgtcggc catggcgtag aacgcggcga gccgccgctg gtccaggatc aggcccagct
     1741 cgatcagcgc cgggcccaag gggtcggcca ggggctggca ccgctcgcgg aagccctcgt
     1801 aggtcaccga cgtctcgccg gcgaagacga aggcgacgtc cggccgggcc gcgagcacct
     1861 tgggcgcggc acgcagcagg acgtcgaacc ccttctcctc cacccagcgg ccggcgaacg
     1921 cgaccaccgg gcggtcaccg aggccgagct ccgaccgcag ctcgtcggtg gccccgggct
     1981 cgggcgggtc gaacaccacc ggcggatcga tggccaaggc acgggtcccg gcgacggccg
     2041 gtgaggcgtg ggcggcgtag tcgtcgttgt gggtgacgac ccgggcggcc tgctggaacg
     2101 tccggcggag ggtcgcccgc atcagccgct ggacgagctg ctccgccggg gtgtcgggca
     2161 tgacgacgtc gccttggtgg gtgacgacca gcggcacgcg gtgccgccgg cacgtcgccg
     2221 cgacgagctc ggcttcggcc atcgggctgt gcaggtgcac cacgtcgtgg tggggcacga
     2281 gccggtggag cacgagcggc aaggccggca tcagcagcgt tcggctgagg cggccagcgg
     2341 tggggacccg cacgatggcc acgccgttgc gctcgtcgcg ccgggacgtc tgcggatcgt
     2401 ggtggctgca cacgacggtg acgtcgcagc cgtcggcggc caggccttcc gcgatgcgcc
     2461 gagcgaacac ggtgagcccc gtccaatgcg ggtcgtagaa ggtcaagacc gtcaagaccc
     2521 gcggcatcgt ccgatggtac gggatgcccg ccttcctaag ctgcggaggt gtcggggctg
     2581 acggtgctct tcctcctggt gtgcgccgcc gccggcttcg ggatcaccgg cctcacccgg
     2641 ttgccgatcc ggttcgagga gcgcgtggcc gtcgccggtc cgctcggcgc gctggcggtg
     2701 ggcctgggcg ggtggggctg gtcggtggcg atcgggttcg accggacgtc gctcggcctc
     2761 gccgtggtca cggccacgct ggtcagcctg ccggggtgga cgcggatcgc gcccgggctc
     2821 ggcgacgagg cccgcggcgc gtggcggcgc gtccggcttc cctggcggga cggcgagagc
     2881 ctccggccgc tcgtcctgct gctcgtggtg gcttggccgg tcaccatccg gatcttcgcg
     2941 ctcgcctgga tggcgagcgg tcagggcggc ctcgacgtcg gccacctcgc cacctacgcg
     3001 gacggcagcg cccacctggc gtacgccggg cggttcgtca gcggcggcga ggtgccgatc
     3061 gcctcgccga tcgcggcggg agagccggcg cgctaccacc tgctggccga cctgttcgcg
     3121 gcgcaggcgt cgctgctcgg cgtcagcttg acctcggccc tggccgtcac gagcgggttc
     3181 ttggccttgt tcttcccggc catcgcctac tgctgcggcg tgcgcctcgt cgccaaccgg
     3241 tgggcggcgc tcgtcggcgt ggtcgtgttc tgcgccggcg ggacgctcgg gttcgtccac
     3301 ctgttcggcg acatcggcga tcacggctgg gggatcctct ggcagctccc gcgcgactac
     3361 tcccgcgacc ccgcatcgct gctgtggatg gacaacccgt cgctgtcgta cctccacgcg
     3421 cagcgcaacg gcctgttcgg gctcccgctg ggcctcgtcg ccgtgacgct gctgcgctgc
     3481 ggcgtcgagg agcgccgcgc cgccgcgttc accgcggccg gcgtgctggt gggcgcggtg
     3541 cccctggcca acgggttcgc cttcgtcgtc ccgatggcca tcgcctcggc gtgggcggtg
     3601 atggaccgcc accggccctg gtggcggttc ttccttcccg cactcgtcct cgggctcccg
     3661 gcggcgtggt ggctccagcc ggcggagtcg tcggtgcgct ggttcccggg gtggatggcc
     3721 gacgacgggg tggggggctg gctggtgttc tgggcgcgca acgccggccc gttcctcgtc
     3781 ttgctggtgg tggcgtgcgc gtggcgggga acggtgcccc ggcgcacggt gcgcgccttc
     3841 ttgccgctgt ggctgctgtg gatcgtgccg aacctcgtgg ccttccaccc gtgggagtgg
     3901 aacaacacca agtacttcgc gtactggcag ctgatcggat cgttcctcgt cggtgccgtg
     3961 ctcgtccggc tggtgcgcga ggggatcgcc ggccgtgtcg ccgcgggggt ggtggtcgcc
     4021 gtcgtggtgg tggtcgccct caccgcgtcg ggtgtcgccg acctgtctcg ggcgatggac
     4081 cggtcggcga cgcgcatccc gtgggcctcg gccgacgcgc tcgaggtggc agcctggctc
     4141 cgggactcga ccgatcccgg ggtcgtggtg gcggcggcgc ccgccatcga cgagccggtg
     4201 gtggcgctgt cgggacgtcg gttcgtgtcc gggttccccg gctggacgtt cgacctcggc
     4261 gtccccgact gggcggctcg ttcggacgac gagcaggcga tcctccgggg tggcgccgac
     4321 gggttggccg cggcgcgccg gcgcggtgtc gacctggtcg tcatcggtcc catcgagcgg
     4381 ggcccgtcgt tccaggccga cgaggcatgg tgggcagcca acgcctcgct cgtccaccgc
     4441 ttcggcgact ggtcggtcta cgcggtgccc cgatgagcgt gccggcccgg gcgggggcga
     4501 gggccgggtt cgtcgtcgtc gccgctgcat cggcggcgta cgcgttcgac caggttcgcc
     4561 accgagcgtt cgcggcgtcg acgtgggaca tcaccctggc gtggggggtc ggcgtcggct
     4621 gcctcgtggg cctcgccgcc gtcggacggg gccgcgccga ggagggcggc gaccggcggg
     4681 gtctgatcgc tcgcggccgg cactggcggc cgacgccgta cgaggctgct ggcctgtcga
     4741 tcctggccgt cgcctcggtc gcccggctgg ccgtgctggg ccggttcccg acggtgatcg
     4801 acggcgacga gggcgtcttc tcgagccagg cggtgctggc tc
//
LOCUS       DATMFS010000369         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_12887, whole genome shotgun sequence.
ACCESSION   DATMFS010000369 DATMFS010000000
VERSION     DATMFS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12887"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VNQ33_06825"
     CDS             <1..947
                     /locus_tag="VNQ33_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWJ97857.1"
                     /translation="PMTTLLDRKLLFVTGKGGVGKSTIAASLGLLAAEQGRRTLVCEV
                     DAKGNLADFYEAGETAFAPRELQPNLWAMSMNTEESLKEYLSLQLKIPLLAKIGPLAR
                     TFDFIANAAPGVKEILTIGKLAWEVKERHYDLVVVDSVASGHIIGQLTAPQGINELVQ
                     VGVVRNQTQWMLDILTDPAQTGVVIVSAPEEMPVAETIELADRLNTETKVDLAAVVVN
                     RVLPELFTEREEALFESLRTPDRIGALSAAADGDVAPVFDAAELAVTLRRTRAEHLAT
                     LRRALDPTLPMLYVPYLFTRSHGARATRKVAELLAEEL"
     gene            947..2143
                     /locus_tag="VNQ33_06830"
     CDS             947..2143
                     /locus_tag="VNQ33_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HWJ97858.1"
                     /translation="MAARGKAPAGAAGSSSERARTLEQLLATKEIVISCGSGGVGKTT
                     TAAAAAAMAATHLGGKVLVLTVDPAKRLANALGLERFGNVEIRVPDAAFAGAGIEPRG
                     ELWAAMLDTKQSWDDLIARHAPDAKTREAILANSIYQNISGRFVQSHDYIAMERLYEI
                     HSSGTYDLIVVDTPPTRNAIDFLEAPDRMADFFSSKLLRWLTAPSKSRVMNFASKPFY
                     TVADRILGSQFLEDIAEFFSLFQAMAPGFVERADAVTKILSDKRTTFVVVSTLEAAPL
                     HEAEFFIDALDERRLHLGALVLNKVLPSYLRRTATTGVAKRLCSDADALAAVVPGAGD
                     PVDAARVLATIGESFLNYQVVAKREAEQRAGLARTPDVVAAVPYFDADIGDLSGLLQL
                     GDQIWR"
     gene            complement(2174..2509)
                     /locus_tag="VNQ33_06835"
     CDS             complement(2174..2509)
                     /locus_tag="VNQ33_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97859.1"
                     /translation="MTITEPARHRLYDKLEGLLGMDDAATLMAHLPPVGWADVATRAD
                     LDQLHDRIHTDLAEIRVEMASLRTDLRTDLAGFRDEIRRDQLQVQRQLIFAVIAAALS
                     LLGTIAIAI"
     gene            complement(2669..>3166)
                     /locus_tag="VNQ33_06840"
     CDS             complement(2669..>3166)
                     /locus_tag="VNQ33_06840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ97860.1"
                     /translation="RAGPDDLGERCHRARLGCPAMVAMGPDALNDFLDAAFPDAQLPM
                     RVTRSDDDGVELSWPYHENQLRPGGTLSGPTLMTLADTVAYVAVVAQIGPQFLTVTSH
                     LDIDFLRKPPPADLRATGELLKLGRRQAVVSIRIYSTADDEIVAASSCTYALPSGPPA
                     DQTAG"
BASE COUNT          471 a         1109 c         1112 g          474 t
ORIGIN      
        1 cgccgatgac cacgctgctc gatcgcaagc tgctgttcgt cacgggcaag ggcggcgtcg
       61 gcaagtccac catcgccgcg tcgctcgggc tgctggccgc agagcagggc aggcgcacgc
      121 tcgtgtgcga ggtcgatgcc aagggcaacc tggccgactt ctacgaggcg ggcgagacgg
      181 cgttcgctcc ccgggagctg cagcccaacc tctgggcgat gtcgatgaac accgaggagt
      241 cgctgaagga gtacctgagc ctccagctga agatcccgct gctggccaag atcggcccgc
      301 tggcgcggac cttcgacttc atcgccaacg ccgctcctgg ggtgaaggag atcctcacca
      361 tcggcaagct ggcctgggag gtgaaggagc gccactacga cctggtggtg gtcgattcgg
      421 tcgccagcgg ccacatcatc ggccagctca ccgcacccca gggcatcaac gagctggtgc
      481 aggtgggcgt cgtccgcaac cagacgcagt ggatgctcga catcctcacc gacccggccc
      541 agaccggggt cgtcatcgtg tcggcacccg aggagatgcc ggtcgcggag acgatcgagc
      601 tggcggaccg gctgaacacc gagaccaagg tcgacctggc cgcggtggtg gtgaaccgcg
      661 tcctgcccga gctgttcacc gagcgggagg aagcgctgtt cgagtcgctg cggacccctg
      721 accgcatcgg cgcgctgtcc gccgccgccg acggcgacgt cgcgccggtg ttcgacgcgg
      781 ccgagctggc ggtcaccctg cgccgtaccc gggccgagca cctggccacg ctccgccggg
      841 cgctggaccc gacgcttccg atgctgtacg tgccgtacct gttcacccgc agccacggcg
      901 cccgcgccac ccgcaaggtg gccgagctgc tggcggagga gctctgatgg cggcgagggg
      961 caaggcgccg gccggggcgg cgggttcgtc cagcgagcgg gcccgcacgc tcgagcagtt
     1021 gctggccacc aaggagatcg tcatctcctg cgggtccggc ggggtgggca agaccaccac
     1081 cgcggcggcc gcggccgcca tggccgccac gcacctgggc ggcaaggtgc tcgtcctcac
     1141 ggtcgatccc gccaagcggc tcgccaacgc cctcgggctg gagcgcttcg gcaacgtcga
     1201 gatccgggtg cccgacgcgg ccttcgccgg tgccgggatc gagccccggg gcgagctgtg
     1261 ggcggcgatg ctcgacacca agcagtcgtg ggacgatctg atcgcccgcc acgcccccga
     1321 cgccaagacc cgcgaggcca tcctcgccaa ctcgatctac cagaacatct cgggccggtt
     1381 cgtgcagagc cacgactaca tcgccatgga gcggctctac gagatccact cgtcgggcac
     1441 ctacgacctg atcgtcgtgg acacgccgcc cacccgcaac gccatcgact tcctcgaggc
     1501 gcccgatcgc atggccgact tcttctcgtc gaagctcctc cgctggctca cggcgccgtc
     1561 gaagagccgg gtcatgaact tcgcgtcgaa gcccttctac acggtggccg atcgcatcct
     1621 cggctcgcag ttcctcgagg acatcgccga gttcttcagc ctcttccagg ccatggcgcc
     1681 cggcttcgtc gagcgggccg acgccgtcac caagatcctc tcggacaagc ggaccacgtt
     1741 cgtcgtggtg tccacgctcg aggcggcgcc gctgcacgag gccgagttct tcatcgacgc
     1801 cctcgacgag cgcaggctcc acctcggtgc cctcgtgctc aacaaggtgc tcccgtcgta
     1861 cctccgtcgc accgccacca ccggcgtcgc caagcgcctg tgctcggacg ccgacgccct
     1921 cgccgcagtc gtgcccggcg cgggcgaccc ggtcgacgcc gcccgggtgc tggccaccat
     1981 cggcgagagc ttcctcaact accaggtggt cgccaagcga gaggccgagc agcgcgccgg
     2041 cctcgcccgc acgcccgacg tcgtcgccgc cgtcccgtac ttcgacgccg acatcggtga
     2101 cctctccggt ctcctccagc tgggcgacca gatctggcgc tgagccagcc tcgtcgatcg
     2161 tcggggcggc gcgtcagatg gcgatggcga tggtgccgag caggctcagc gccgcggcga
     2221 tgacggcgaa gatcagttgc cgttgcacct ggagctgatc gcggcggatc tcgtcgcgga
     2281 agccggcgag gtcggtgcgc aggtcggtgc gcaaggaggc catctcgacg cggatctccg
     2341 cgaggtcggt gtggatgcgg tcgtggagct ggtcgaggtc ggcgcgggtg gctacgtcgg
     2401 cccagccgac gggtgggagg tgggccatca gcgttgcggc gtcgtccatg cccagcagtc
     2461 cttcgagctt gtcgtacagc cggtgcctgg ccggctcggt gatcgtcatg gtgccatccc
     2521 tggaacgggc cggatcggat gcttcgggcc gaccagagag ccgaagtgac cgccagggga
     2581 tgacggcgag gtcgaggcga cggtaccgcg gcgccgccgc ggggtcaacg ggccttgacc
     2641 tgcggacgcg gccgtgtggt cggatcggtc acccggcggt ctggtcagct ggcggtccgc
     2701 tcggcagggc ataggtgcag ctgctggcgg ccacgatctc gtcgtccgcg gtcgagtaga
     2761 tgcggatgct gaccaccgcc tgacggcgac cgagcttgag cagttcgccg gtggcccgga
     2821 ggtcggcggg cggcggcttg cggaggaagt cgatgtcgag gtggctcgtc accgtgagga
     2881 actgcgggcc gatctgcgcc acgaccgcga cgtaggccac cgtgtcggcc aaggtcatca
     2941 aggtcgggcc tgacagcgtg ccgccgggtc ggagctggtt ctcgtggtac ggccacgaca
     3001 gctcgacgcc atcgtcgtcg gaccgggtga cccgcatcgg cagctgggca tcggggaacg
     3061 ccgcgtcgag gaagtcgttc agcgcgtcag gacccatggc gaccatggcc gggcacccta
     3121 acctcgccct gtggcatcgc tcgccgagat cgtccggacc cgctcg
//
LOCUS       DATMFS010000370         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_244316, whole genome shotgun sequence.
ACCESSION   DATMFS010000370 DATMFS010000000
VERSION     DATMFS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244316"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VNQ33_06845"
     CDS             complement(<1..534)
                     /locus_tag="VNQ33_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97861.1"
                     /translation="MSGSSNPGPDSHPDANGANANGSNVNGSNVNGQNGKDHTEGTDP
                     AEVGGKALQRQAMAIEEEAAAVETAPEPYRPPAAETRQAGPRFGAAGMPAEKSKDFRG
                     STGRLLARLRPQRGGLIAVLFLAIFSVTLSVLGPRLLGNATNIIVDGVISGQGIDFGK
                     LHRTLAGVLALYVVSATA"
     gene            complement(531..2267)
                     /locus_tag="VNQ33_06850"
     CDS             complement(531..2267)
                     /locus_tag="VNQ33_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97862.1"
                     /translation="MLLIRLLLSYLKRYRKILLAVVGLQIVATAAALTLPTLNADIID
                     KGVLTGNTGYIWKVGGLMLLVTLVQVVFNVGAVFFGSKAAMGFGRDVRKDLFHKVTGF
                     STQEVAALGAPSLITRITNDVTQVQMLVLMSCTLLVTAPITIVGGVIMAVREDGPLSL
                     LLLVSVPALIISVGSVIWRLVPTFGVMQVRIDRVNQVMREQITGMRVVRAFIREPHEK
                     ARFAVANDDLTETSLRAGRLMAFMFPTVMFILNASSVAAIWFGADRIDSGSMQIGALI
                     AFLTYLVQILMSVMMATFVAVMAPRAAVCAERIDEVLETPFSIVRADAPVTELPTSVG
                     LELRNVSFAYPGAESPVLCDISFTAPAGTTTAIIGSTGSGKTTLLNLVPRLFDATSGQ
                     VLVNGIDVRDIEPEVLWAQTGIVPQKPYLFSGTVASNLRYADPDATDAELWEALEIAQ
                     ASSFVRDMPEGLDSTIAQGGTNVSGGQRQRLAIARALVRKPGIYLFDDSFSALDLATD
                     ARLRAALGPVTRNSAMIVVAQRVSTIERADQILVLEDGETVGLGTHEELLASCPTYQE
                     IVASQRTEEEAA"
     gene            complement(2284..>2800)
                     /locus_tag="VNQ33_06855"
     CDS             complement(2284..>2800)
                     /locus_tag="VNQ33_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97863.1"
                     /translation="RVFDDKTALLDAVVEAAVDPAPSEAALRAIDPALPLEDRLVQAV
                     DILRRRIADVFQIMSAVGAMPGPRTRASKPRGPELLVLAEIFGSDAPRLRREPAQAAQ
                     VLRGLTLAGTHPLLILDEPLSSEEIVSLLLDGIRARPDEPVLAPLTEDAIAAFTRGDL
                     DFDPTRTPEPN"
BASE COUNT          428 a          915 c         1050 g          407 t
ORIGIN      
        1 ggcggtggcc gacacgacgt agagcgcgag cacccccgcc agggtgcggt gcagcttgcc
       61 gaagtcgatc ccctgcccgc tgatgacgcc gtcgacgatg atgttcgtgg cgttgccgag
      121 cagccggggg ccgagcaccg acagcgtgac gctgaagatg gcgaggaaca gcacggcgat
      181 gagcccaccc cgctgcgggc ggagacgggc gagcaggcgc ccggtggagc cgcggaagtc
      241 cttggacttc tccgcgggca tgccggccgc accgaagcgg gggccggcct gacgggtctc
      301 agccgccggg ggacggtacg gctcgggcgc ggtctcgacc gccgccgcct cctcctcgat
      361 cgccatggcc tggcgctgca gcgccttccc cccgacctcg gccgggtcgg tgccctcggt
      421 gtggtccttg ccgttctggc cgttcacgtt gctgccgttc acgttgctgc cgttcgcgtt
      481 cgcgccgttg gcgtcggggt gggagtcggg gccggggttg gacgagccgc tcatgccgcc
      541 tcctcctcgg tgcgctggga cgcgacgatc tcctggtagg tcggacagga tgcgaggagc
      601 tcctcgtggg tcccgagccc gaccgtctcg ccgtcctcga gcacgaggat ctggtcggcc
      661 cgctcgatgg tggacacccg ctgggcgacg acgatcatcg ccgagttgcg ggtgaccggg
      721 ccgagtgcag cccgcaggcg ggcatcggtc gccaagtcga gcgcagagaa cgagtcgtcg
      781 aacaggtaga tcccgggctt gcggaccagg gcccgggcga tggccaggcg ctggcgctga
      841 ccgcccgaca cgttggtacc gccctgggcg atggtggagt ccagcccctc gggcatgtcc
      901 cggacgaacg aggaggcctg ggcgatctcg agcgcctccc acagctcggc gtcggtggcg
      961 tccgggtcgg cgtagcgcag gttgctggcc acggtgcccg agaacaggta cggcttctgc
     1021 gggacgatcc cggtctgggc ccacagcacc tcgggctcga tgtcgcgcac gtcgatgccg
     1081 ttcacgagga cctgccccga ggtggcgtcg aagaggcggg gcaccaggtt gagcagcgtc
     1141 gtcttgcccg agccggtgct gccgatgatc gccgtggtgg tgccggcggg tgcggtgaag
     1201 gagatgtcgc aaaggaccgg gctctcggcc cccgggtagg cgaacgagac gttgcgcagc
     1261 tcgaggccga ccgacgtcgg cagctcggtg acgggcgcgt cggcccgcac gatgctgaac
     1321 ggcgtctcga gcacctcgtc gatccgctcg gcgcagacgg cggcacgggg cgccatgacg
     1381 gccacgaagg tggccatcat caccgacatc aggatctgca cgaggtaggt gaggaacgcg
     1441 atcagcgcac cgatctgcat gctccccgag tcgatgcggt ccgcaccgaa ccagatggcc
     1501 gccacgctcg acgcgttcaa gatgaacatg accgtgggga acatgaacgc catcagccgg
     1561 ccggcccgca gcgaggtctc ggtgaggtcg tcgttggcca ccgcgaagcg agccttctcg
     1621 tggggctcgc ggatgaacgc ccgcacgacg cgcatgccgg tgatctgctc gcgcatgacc
     1681 tggttgaccc ggtcgatgcg gacctgcatc acgccgaagg tcggcacgag ccgccagatc
     1741 accgagccga cgctgatgat gagcgccggc acgctcacga ggagcagcag ggagagcggc
     1801 ccgtcctcgc gcacggccat gatcacgccg ccgacgatcg tgatcggagc ggtgacgagc
     1861 agcgtgcacg acatcaagac cagcatctgg acctgggtca cgtcgttggt gatgcgggtg
     1921 atgagcgacg gggctccgag cgcggcgacc tcctgcgtcg agaagccggt gaccttgtgg
     1981 aacaggtcct tgcggacgtc gcggccgaag cccatggccg ccttcgaccc gaagaacacg
     2041 gcgccgacgt tgaacaccac ctgcaccagc gtgaccagca gcatcagccc gccgaccttc
     2101 cagatgtagc cggtgttccc ggtgagcacg cccttgtcga tgatgtcggc gttcagcgtc
     2161 gggagggtca gcgccgccgc ggtggcgacg atctggaggc cgacgaccgc caacaggatc
     2221 ttgcggtagc gcttgaggta cgagaggagc aggcggatga gcaacatggc ggttcccggt
     2281 ggttcagttc ggttcgggcg tacgggtggg gtcgaagtcg aggtcgcccc tcgtgaacgc
     2341 ggcgatcgcg tcctccgtga gcggtgcgag gacgggttcg tcgggtcggg cgcggatgcc
     2401 gtcgaggagg agcgacacga tctcctccga cgacagcggc tcgtcgagga tgagcagggg
     2461 gtgggtgccg gcgagggtga ggccccggag gacctgggcc gcctgtgccg gttcgcggcg
     2521 cagccggggt gcgtcggacc cgaagatctc agccagcacg agcagctcgg gtccacgggg
     2581 tttcgacgcc cgggtccggg gccccggcat ggcgccgacc gccgacatga tctggaagac
     2641 gtcggcgatg cgccgccgca agatgtcgac ggcctggacg agccggtcct cgagcgggag
     2701 cgccgggtcg atcgcccgca gcgccgcctc ggagggggcc gggtccactg cggcctcgac
     2761 gaccgcatcg agcagcgccg tcttgtcgtc gaagacccgg
//
LOCUS       DATMFS010000371         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_292098, whole genome shotgun sequence.
ACCESSION   DATMFS010000371 DATMFS010000000
VERSION     DATMFS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292098"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VNQ33_06860"
     CDS             <1..376
                     /locus_tag="VNQ33_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97864.1"
                     /translation="NGKAGDPVVRQRLAAAQSGLDIMRWNTLRVLSGPDDAARVPPAA
                     MVTKLYWANWHRDLGELAMDVLGPEALLGDPSVAPGEGYELTALQRLFLFTRSDTIYA
                     GSNEIQRNIIGERALGLPKEPA"
     gene            373..1155
                     /locus_tag="VNQ33_06865"
     CDS             373..1155
                     /locus_tag="VNQ33_06865"
                     /inference="COORDINATES: protein motif:HMM:NF005880.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ97865.1"
                     /translation="MTQPAPEYVGGHGLLAGKTVVVTAAAGTGIGSAVARKAIEEGAT
                     VMISDFHERRLGETADRLADETGTRPDTQACNVTDEAQVQALVDAAVDRLGRLDVMIN
                     NAGLGGSAPIVEMTDEQWTTVLDVTLTGTFRCTRAVLKQMYEQGSGVIVNNASVLGWR
                     AQAEQAHYAAAKAGVMAFTRCAAIEAAPHGVRINAVAPSLAMHPFLAKVTTDELLAEL
                     TEREAFGRYAEPWEIANVMVFLASDYSSYLTGEIISASSQHP"
     gene            1191..1667
                     /locus_tag="VNQ33_06870"
     CDS             1191..1667
                     /locus_tag="VNQ33_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWJ97866.1"
                     /translation="MAATTFDGIDAVRAAAGTHLGYSDWTTVTQEQVNTFAEATGDFQ
                     WIHVDVERANAESPFGGPIAHGYLTVSLTNLFLPQVVEVRNISAGLNRGTGKIRFPAP
                     VPVGSRLRGGVELVAVDDVERDGAVTGIDTTMIITVEREGGDKPVCVVEALSRYLA"
     gene            2202..>2421
                     /locus_tag="VNQ33_06875"
     CDS             2202..>2421
                     /locus_tag="VNQ33_06875"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97867.1"
                     /translation="MAVSVVVFTAVNWSQLDASLQGLILVALTIVAGAAAAGAVRRDM
                     PSTAEAVGAVAVLMALADVHAVRVGLAPA"
BASE COUNT          332 a          954 c          827 g          308 t
ORIGIN      
        1 caacgggaag gccggcgatc ccgtcgtgcg ccagcgcctc gccgccgccc agagcgggct
       61 cgacatcatg cggtggaaca ccctccgggt gctctccggt cccgacgacg ccgcccgcgt
      121 gccccctgcc gccatggtca ccaagctgta ctgggccaac tggcaccgcg atctgggcga
      181 gctggccatg gacgtcctcg gccccgaggc cctgctgggc gatccgtcgg tggcgccggg
      241 cgagggctac gagctcaccg ccctgcagcg gctcttcctc ttcacccgct cggacaccat
      301 ctacgccggg tccaacgaga tccagcgcaa catcatcggc gagcgcgcgc tcggcctgcc
      361 caaggagccc gcatgaccca gcctgcaccc gagtacgtgg ggggccacgg gctcctcgcc
      421 ggcaagaccg tcgtcgtcac cgccgcggcg ggcacgggca tcggctccgc cgtggcccgc
      481 aaggccatcg aagagggcgc cacggtgatg atctcggact tccacgagcg ccgcctcggc
      541 gagaccgccg accgtctggc cgacgagacc ggcacccggc ccgacaccca ggcctgcaac
      601 gtcaccgacg aggcccaggt ccaggccctg gtcgacgcgg cggtggaccg cctcgggcgc
      661 ctcgacgtca tgatcaacaa cgcggggctc ggtgggtcgg cccccatcgt cgagatgacc
      721 gacgagcagt ggacgacggt cctcgacgtc accctcaccg gcaccttccg ctgcacccga
      781 gccgtcctca agcagatgta cgagcagggg agcggcgtca tcgtcaacaa cgcctcggtc
      841 ctcggctggc gggcgcaggc cgaacaggcg cactacgccg cggccaaggc cggggtcatg
      901 gccttcaccc gctgcgccgc catcgaggcc gcgccgcacg gcgtgcgcat caacgccgtc
      961 gccccgagcc tggccatgca cccgttcctc gccaaggtca ccaccgacga gctgctcgcc
     1021 gagctgaccg agcgcgaggc cttcggccgc tacgccgaac cgtgggagat cgccaacgtg
     1081 atggtcttcc tcgccagcga ctactcgagc tacctcaccg gcgagatcat ctccgccagc
     1141 tcccagcacc cctgaccccg cccaccggca ccacgaacga ggacgacgac atggctgcga
     1201 ccaccttcga cggcatcgac gcggtccgcg ccgcggccgg cacccacctc gggtacagcg
     1261 actggacgac cgtcacccag gagcaggtga acacgttcgc cgaggccacc ggggacttcc
     1321 agtggatcca cgtcgacgtc gagcgggcca acgccgagtc gcccttcggc gggcccatcg
     1381 cccacggcta cctcacggtc tcgctcacca acctcttcct gccgcaggtg gtcgaggtgc
     1441 ggaacatctc ggccggcctc aaccgcggca ccggcaagat ccgcttcccg gcgccggtcc
     1501 cggtcggctc ccgcctgcgc ggcggtgtcg agctggtggc cgtcgacgac gtcgagcgcg
     1561 acggcgcggt cacggggatc gacacgacga tgatcatcac cgtcgagcga gagggcggcg
     1621 acaagccggt gtgcgtggtc gaggccctga gccgctacct ggcctgaggc cggttgcccc
     1681 gggccgccgc tggcggacgg cgacgaaggc gcgccgggcg ccggtcatga cgccgggtgc
     1741 accgattggg gggttccccc agggggttct gggggcgagc gcccagggcg gggaggcggg
     1801 gtcgggcgga ccatggcggc atgaccccca cccctgcccg ctcgccctgg ccgaacccgg
     1861 ccgcccacga tcccggctgg gggccgccgc ccccgacgcc acccccggcg ccgccgtcga
     1921 tcccgcacct gcccccgccg ccagacccgg gttgggaccg cccggctccg ccgcccgccc
     1981 cgtccgagtg ggtcccgcct tcgcagacca ggcccggatc gccgttcccg cccgctccgc
     2041 ccaccgactg gcctggaggt gcagccgctc atcccggtgc gcccggcggg ggtgtcccaa
     2101 gcggcccggg ctcgtgggtt ccgacgccgc cggacccgtt cgagccgtcg cggcggccgg
     2161 tcggcctgca gaacctgctg ctcggcctcg gtaccgccct ggtggccgtc tcggtcgtcg
     2221 tcttcaccgc cgtcaactgg agccagctcg atgcgagcct gcagggactg atcctggtgg
     2281 cgctcacgat cgtcgccggt gctgctgccg ctggagcggt ccggcgggac atgccgtcga
     2341 cggccgaggc ggtcggagcg gtggccgtgc tgatggcgct cgccgacgtc cacgcggtcc
     2401 gggtcgggct ggccccggcc g
//
LOCUS       DATMFS010000372         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_221921, whole genome shotgun sequence.
ACCESSION   DATMFS010000372 DATMFS010000000
VERSION     DATMFS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221921"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..817
                     /locus_tag="VNQ33_06880"
     CDS             149..817
                     /locus_tag="VNQ33_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97868.1"
                     /translation="MAIDVETKDCTQLGDAELAEMADLCADAPSRFEVGVLSKAAEAW
                     VLVTLAREAGKLKGFSFSTLERIGGTPSVIVGLASIKRTSKRDTVLRAIVHDQLRRAV
                     LAFPDEDVLVGTRFATVSGYEAFKSLVDIVPRPDYLATGEERAWGRRLAKRFGINASR
                     YDERSFIVRGDRSYPEVLDHESLKPEKLDAGFEAFFVEVDEARGDALIAFGWATADDL
                     LKLT"
     gene            858..1457
                     /locus_tag="VNQ33_06885"
     CDS             858..1457
                     /locus_tag="VNQ33_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWJ97869.1"
                     /translation="MHLDDVIRGRRMVRSFTGEAVPADLLDALCDLARRAPSAGNSQG
                     LDLLVLRGPAEVGAYWDVTLPSPRRAGFRWPGLVAAPALVLVVTDPAAYVARYGEDDK
                     ASTGLGTGADAWPVPYWWVDAGAAIEHLLLGAVDAGLGACLFGLFGHEQAVADAFGVP
                     PGRRIVGTIALGRPAPDEPGRSARRPRRGLDDVIHRGCW"
     gene            1830..>2173
                     /locus_tag="VNQ33_06890"
     CDS             1830..>2173
                     /locus_tag="VNQ33_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97870.1"
                     /translation="MNKAVARAKRDEQPPSTDGTSTGRARLWPVAVALVAVGLVAAAA
                     QVSSADPTTAGAVAATTQRTHGTVPAARPAQAVAEAPASSVFAELGDITLVGAATAAT
                     AASDLDVTPPPPP"
BASE COUNT          273 a          791 c          782 g          327 t
ORIGIN      
        1 accctcgcat cctcgcgcag cgcgtctgcg cgctggggca ccccggtgcg ggcgccttcc
       61 gcccgggcgc tcaagttgcg tcagctggcc tggtcgactg aacttggagg ccacgtgtcc
      121 accgtgtgaa ggggttcagg accggctcat ggctatcgat gtcgagacca aggactgcac
      181 gcagctgggc gacgcggagc tggccgagat ggcggacctg tgcgccgacg ccccgagtcg
      241 cttcgaggtc ggggtcctct ccaaggccgc cgaggcctgg gtgctcgtga ccttggcccg
      301 agaggccggg aagctgaagg ggttctcgtt cagcaccctc gagcgcatcg gcggcacgcc
      361 gagcgtcatc gtcgggctgg cctccatcaa gcgcacgtcc aagcgcgaca ccgtcctgcg
      421 ggcgatcgtc cacgaccagc tgcggcgggc cgtgctggcc ttccccgacg aggacgtcct
      481 cgtcggcacc cgcttcgcca ccgtgtcggg ctacgaggcc ttcaagtcgc tcgtcgacat
      541 cgtgccgcgg cccgactacc tcgccacggg cgaggagcgg gcgtggggcc gccgcctggc
      601 caagcgcttc ggcatcaacg ccagccgcta cgacgagcgt tcgttcatcg tccgcggcga
      661 ccgcagctac cccgaggtgc tcgatcacga gtccctgaag cccgagaagc tcgacgccgg
      721 gttcgaggcg ttcttcgtcg aggtcgacga ggcccggggc gacgcgctca tcgcgttcgg
      781 ctgggccacc gccgacgacc tcctcaagct gacctgatct ggcggttgcg gcacggtccg
      841 cgccgatcgg tcgcctgatg cacctcgacg acgtcatccg cgggcggcgg atggtccggt
      901 cgttcacggg cgaagccgtc ccggccgacc tgctcgacgc gctgtgcgac ctggcccggc
      961 gagcgccttc ggccggcaac agccaggggc tggacctcct ggtcctgcgc gggcccgcgg
     1021 aggtcggcgc gtactgggac gtcaccctcc cctccccgcg ccgggccggg ttccggtggc
     1081 cgggcctcgt cgccgctccg gcgctcgtgc tcgtcgtgac cgatccggcg gcgtacgtgg
     1141 cgcgctacgg cgaagacgac aaggcatcca ccggtctggg aacgggagcg gacgcctggc
     1201 ccgtgccgta ctggtgggtc gatgcgggcg ccgccatcga gcacctcttg ctgggtgcgg
     1261 tcgatgccgg cctcggtgcg tgcctcttcg ggctgttcgg ccacgagcag gccgtggccg
     1321 acgcgttcgg cgtgccgccg gggcgccgca tcgtggggac gatcgccctc gggcggcccg
     1381 ctcccgatga accggggcga tcggcccgac ggcctcgccg gggcctcgac gacgtgatcc
     1441 accggggctg ctggtaggtc tgcccccccg tcacgtcggg ccggttcgtg gggattcggt
     1501 gcagatgggc acgggacctg catcggatcc ggtcgtcgcg atcgggagca ggggaccggt
     1561 gcgggcgggc gatccgtgcc gtcgcggccg ccggtgcgct ggtcagcgcg gtcggcgtgc
     1621 ggttcgaccg gcggcctgac gggttcgtct gctccgatcc ggtcgatcgg tgaaatcgat
     1681 gacctatggc atcgagatcg catgtgacga ttctcacgtg tcgattgggt tacggaatcg
     1741 tggaatgcat gacacaatga ccaccccctg tgggcgcagc ggtgctccat gcccccgccc
     1801 acccccgtct ccgcaaaggg agctttccaa tgaacaaggc agtggcgcgc gccaagcgcg
     1861 acgaacaacc tccgagtacc gacggcacgt ccaccgggcg tgcccgcctc tggcccgtgg
     1921 cggtcgccct ggtggcggtc ggcctcgtgg ctgccgccgc acaggtctcg tcggccgacc
     1981 ccaccaccgc cggtgcggtg gccgccacca cccagcgcac ccacggcacc gtgccggcgg
     2041 cccgcccggc gcaggccgtg gccgaggcgc cggcgagctc ggtgttcgcc gagctcggtg
     2101 acatcaccct ggtgggcgcg gcgaccgccg cgaccgcggc gagcgacctc gacgtgacgc
     2161 ccccgccccc gcc
//
LOCUS       DATMFS010000373         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_199058, whole genome shotgun sequence.
ACCESSION   DATMFS010000373 DATMFS010000000
VERSION     DATMFS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199058"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VNQ33_06895"
     CDS             <1..608
                     /locus_tag="VNQ33_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97871.1"
                     /translation="DEGMIAVEYPDVPDAEILTHTEIRRFLADDLSTAALDAEVGVVT
                     AFVRELAEGQSILVPDPARGEVVVGTITGRYEFHGDVAGELGKHRRAVGWIARHPIAE
                     LPSAVQGVARQKLTLQQHRDAEWSGYLRQVRDGEIGRDPRDRPAGTTSTTTRSSVRST
                     TPRAPRVPKKPAPVTQKTCPGCFLQTHPDRFIGDYCEDCAG"
     gene            687..1421
                     /locus_tag="VNQ33_06900"
     CDS             687..1421
                     /locus_tag="VNQ33_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HWJ97872.1"
                     /translation="MSEPTHHPSGPLAVAAALAASAGFVDAHVYVNVTPVFVANMSGN
                     LIHLGILIGDASWRTALASFLTLAAFTTGVVLATVHHDWEVAHARPVRPSFLLGVEAA
                     LLLALAAWMTTASIGFSAQPRPIDYPVLLVAGVAMGLQASALRRVGQIAVATTYGTGA
                     IVRIGEKLVLAARRADRTTEHRRRVAIAVLTIVLACYLGGAAAATVAGRNAALLAVPG
                     LVIAACALASRTPLPDAEPDREPVSS"
     gene            1574..1879
                     /locus_tag="VNQ33_06905"
     CDS             1574..1879
                     /locus_tag="VNQ33_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWJ97873.1"
                     /translation="MLARLSSRSEAIAEACRRHDATALTVFGSAATDTYDDDSDVDLL
                     VSFAPDSPLSRFDAYFGLKEALEEILLRPVDLVTPSALENPFFARSVEASRIDLYAA"
     gene            2223..2537
                     /locus_tag="VNQ33_06910"
                     /pseudo
     CDS             2223..2537
                     /locus_tag="VNQ33_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2632..>3227)
                     /locus_tag="VNQ33_06915"
     CDS             complement(2632..>3227)
                     /locus_tag="VNQ33_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97874.1"
                     /translation="PRDDTWDFPPPPPVGTRRYLDSLGGPARYGTGDPSIDLNAPLLA
                     HCYAAASALRCSERAAGAVDRLSPALGATPGPTVVWSAQAALRQFYLELALRSGREVS
                     FYFLERVLSQVSTSRDEEEAAALIDSFDHVAAVTQAVGRLGIEQRHMRLFGLSIERWS
                     LLPFETDPTGTFYDSFGSAAERDIDLSLRWGQLVPPR"
BASE COUNT          515 a         1180 c         1025 g          507 t
ORIGIN      
        1 tcgatgaggg catgatcgcc gtcgagtacc ccgacgtgcc cgatgccgag atcctcaccc
       61 acaccgagat ccgccggttc ctcgccgacg acctgtcgac agcggcgctc gacgccgagg
      121 tcggcgtggt gacggcgttc gtgcgggagc tggccgaggg ccagtccatc ctcgtgcccg
      181 accctgcccg tggcgaggtc gtcgtcggca cgatcaccgg ccggtacgag ttccacggtg
      241 acgtcgccgg cgagctcggc aagcaccgcc gcgccgtcgg ctggatcgcc cgccacccga
      301 tcgccgagct gcccagcgcg gtccagggcg tcgcccgcca gaagctcacg ctccagcagc
      361 accgcgacgc cgagtggtcc ggctacctcc gccaggtccg cgacggcgag atcggccgcg
      421 acccgaggga tcgtcccgcc gggaccacgt cgaccacgac ccgctcgtcg gtccgctcca
      481 ccacacctcg ggcgccgcga gtcccgaaga agcccgcgcc ggtcacccag aagacctgcc
      541 ccggatgctt cctccagacc caccccgatc gcttcatcgg cgactactgc gaagactgcg
      601 ccggctgacc cccgctgcac tcaccggaag atgggcctgt ccggcggctg ccgcgctaca
      661 acggcctcga gccgaacgga gcctgcgtgt ccgagccaac ccaccatcca tccggcccgc
      721 tggccgtcgc cgcggcgctc gccgcctccg ccggcttcgt cgacgcccac gtgtacgtca
      781 acgtcacgcc ggtgttcgtc gccaacatga gcgggaacct catccacctc gggatcctca
      841 tcggcgacgc ttcctggcgg acggctctgg cctcgttcct cacgctggcc gcgttcacga
      901 ccggcgtggt gctcgccacg gtccaccacg actgggaggt ggcgcacgcc cggccggtgc
      961 ggcccagctt cctcctcggc gtcgaagccg ccctgctgct ggcgctggcg gcctggatga
     1021 ccacggcatc catcgggttc tccgctcagc cccggcccat cgactatccc gtgctcctcg
     1081 tcgcaggggt cgccatgggc ctccaggcgt cggcgcttcg ccgggtgggg cagatcgccg
     1141 tcgccaccac ctacggcacg ggggccatcg tccgcatcgg cgagaagctc gtcctcgctg
     1201 cccgtcgggc ggaccggacc accgagcacc ggcggcgggt cgccatcgcg gtgctcacga
     1261 tcgtcctcgc ctgctacctc ggtggggcgg ccgccgcgac ggtcgcaggc cgcaacgcag
     1321 ccctcctcgc cgtccccggc ctcgtcatcg ccgcctgtgc gctcgccagc cgcaccccgc
     1381 tccccgatgc ggagcccgac cgggaaccgg tcagctcttg accttctcgc tgacgactcg
     1441 gctggaaccg ccgggttgca tggtgacgcc gtagaggcag tcggcggctt cctcgtgcgc
     1501 accagagcgg agcgacgccg acccgatctt cgattccaac tcgaggggtg catcgatgcg
     1561 gcacactggc gggatgctgg cgcgactatc cagtcgaagc gaggcgatcg ccgaggcgtg
     1621 ccgacgacac gacgcgactg ccctgaccgt gttcggatca gcggctacag atacctacga
     1681 cgatgacagc gacgtcgacc tgctggtctc atttgcaccc gactcgccgt tgagccgctt
     1741 cgacgcctac ttcggcctca aggaggcgct cgaagagatc ctgctccgac cggtcgactt
     1801 ggtcacacca tcggcgctgg aaaacccgtt cttcgctcgg tccgtcgaag catccaggat
     1861 cgacctctat gccgcgtgat cccgctgcat acctctggga cacctccggc agcgcatcaa
     1921 cgcctgtgag aagaaccatc gagaagcggt caggatccac gaggcgcggg cgcggacgta
     1981 tgagtcccgt ggcgtggtgg gaatcgggtc cgccatgagg tgagccaccg gcgtgatcct
     2041 ggatgtgtac ctaccaagtg cacaccaagg agaacacgcc gatggctctg tctgagtctg
     2101 ccgtttccga cctgctcgac gcgttgcgcg tcggggaggg gcaccaacct cgtccgtgag
     2161 ctggcccagt gggcgctcca gcagctcatc gacgccgagg cgaccgagaa gatcggcggc
     2221 gcgtgcggct ggtgatctcc gacgcccaca ccggtttgaa ggctgctatc gccaagtgct
     2281 tcgccgggtc gtcgtggcag cgctgccggg tccacttcgc ccgcaacctc ctcgccacag
     2341 cgccccgagc ccacagcgag ttcgccgccg cgttccggtc gatcttcgcc ctcggtgacg
     2401 ccaaggaagt ctcggcgcgc tggaacgaag tcgagaccac cctcaccgag cggttcccca
     2461 aggccaccgc gctcatgcgc gacgccaaga ccgacgtcct cgcgttccca ggtcgcccgc
     2521 gaaccggcta caactagctc ttgaccttct cgctcacgac ccttgagctg ccgccgggtt
     2581 gcatggtgac gccgtatcgg cagtcggcgg cttccatggt gcgccatccg atcatcgtgg
     2641 cgggaccagt tgcccccaac gtaggctcaa gtcgatatct cgctctgctg cgcttccgaa
     2701 ggagtcgtag aaggttccgg tcgggtcggt ctcgaaggga aggagcgacc atcgttcgat
     2761 gctgaggcca aagagccgca tgtggcgctg ctcaatgccc aatcttccaa ccgcttgcgt
     2821 gaccgcagcg acatggtcaa aagaatcgat cagggcggcg gcctcctcct catccctgct
     2881 tgttgagact tgggagagaa cgcgttccaa gaagtagaag gagacctccc gcccgctcct
     2941 cagtgctagt tccaggtaga actgccgcag cgccgcctgg gccgaccaga cgacagtggg
     3001 acctggcgtg gcacccaacg ccggagacaa ccgatccacg gctccagcag cacgctcgga
     3061 acagcgcaga gccgacgccg ccgcatagca gtgagctagg aggggggcat tcagatcgat
     3121 cgaagggtct cccgtcccgt accgagcagg tcccccaagg ctgtcgaggt agcgacgagt
     3181 tcctacgggc ggcgggggtg ggaagtccca ggtgtcgtcc ctgggag
//
LOCUS       DATMFS010000374         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_82684, whole genome shotgun sequence.
ACCESSION   DATMFS010000374 DATMFS010000000
VERSION     DATMFS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82684"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1087
                     /locus_tag="VNQ33_06920"
     CDS             224..1087
                     /locus_tag="VNQ33_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWJ97875.1"
                     /translation="MTARTMPGGPVADAVFADLAPRIAALIAGGHTPGLGTILVGGDS
                     ASAGYIRMKMEKAESLGFTSPHIHLGDDASQADVLAAVREMNDADDVDAVLIQHPTPP
                     QIDFDAALLALDPAKDVDGLHPVNMGRLALGMPGPVPCTPAGIEALLAHYEIPVSGQE
                     VCILGRGTTLGRPLALLLSQKRPTANAAVTVVHTGVANWADYTRRAGIVIAAAGVPGI
                     LQPEHISPGAVVVGGGVRYEGRRLLPDVDERCEAVAGAITPRVGGVGPTTIALLFRNA
                     VEAAESRARLG"
     gene            1255..2112
                     /locus_tag="VNQ33_06925"
     CDS             1255..2112
                     /locus_tag="VNQ33_06925"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWJ97876.1"
                     /translation="MTRISDLLAKGPTWSFEFFPPKTPEGMTSFDRAVTDLSALEPAY
                     VSITYGAGGSARDTTYDLVVRVNEEQAFPAMPHLTCVGHTRAELIDLLESYRANGVDN
                     VLALAGDPPKDGSDPGGDFVYATELVALAREVGDFSVGVAAFPEVHPRSTDRATDRRF
                     LAAKLNAADFGMTQFFWDADHYRVMVEELAALGCTTPVLPGVMPFVSVAGARRMSGMN
                     NTDIPAWLQERMDEVDGDAEATRRLGVEVATGLIEQLLEVGVPGIHLYAMNRAQSIQE
                     IYDNLGLRR"
     gene            complement(2158..>2626)
                     /locus_tag="VNQ33_06930"
     CDS             complement(2158..>2626)
                     /locus_tag="VNQ33_06930"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWJ97877.1"
                     /translation="PKHVQQFEKENHLRWDSLGEFLALQASLEHLAEFAANPRAQILA
                     DALDAATGRFLDEGRSPSRKAGEIDNRGSHYYLARYWAEELAAQAADTEIAARFAPVA
                     AALVDGEAAIMAELNGVQGSPVDMGGYYSPDDAKVSAAMRPSPTLNAAIALLA"
BASE COUNT          365 a          957 c          922 g          382 t
ORIGIN      
        1 atcgcccggg ccgacgagct gggtgtggcc atggtcttca ccggtgagcg ccagttcaag
       61 cactgacgcc gccctcccac ccggatcgcc gggacgacct cggcccgtcg gccggtgcgc
      121 gtcgaaccgt gcgcgtcgaa tcgacgtcga ttcgacgccc ccgctcgggg ttgaggagca
      181 tgggtgccgg accggagcca cgaccccgag aaggagcgat cccttgaccg cacgcacgat
      241 gcccggtggg ccggtggccg acgccgtctt cgccgacctc gccccacgga tcgcggcctt
      301 gatcgcaggc gggcacaccc cggggctggg gacgatcctc gtcggcggtg attcggccag
      361 cgccggctac atccgcatga agatggagaa ggccgagtcg ctcggcttca cgtcgccgca
      421 catccacctc ggcgacgatg cctcgcaggc cgacgtgctg gccgccgtcc gggagatgaa
      481 cgacgccgac gacgtcgacg ccgtgctcat ccagcacccc acgccgccgc agatcgactt
      541 cgatgccgcc ctgctcgcgt tggatccggc gaaggacgtc gacggtctcc acccggtgaa
      601 catgggtcgg ctggcgctcg gcatgccggg gccggtgccc tgcaccccag ctggcatcga
      661 ggcgctcctg gcgcactacg agatcccggt gtcgggccag gaggtctgca tcctcggccg
      721 tggcacgacc ctcgggcgtc cgctcgccct gctcctgtcg cagaaacggc cgacggccaa
      781 cgccgccgtc accgtcgtcc acaccggcgt ggcgaactgg gccgactaca cgcggcgtgc
      841 cggcatcgtg atcgccgccg ccggggtacc gggcatcttg cagcccgagc acatctcgcc
      901 cggggcggtc gtcgtcggcg gcggtgtccg ctacgagggc cggcgtctcc tgcccgacgt
      961 cgacgagcga tgcgaggccg ttgccggcgc gatcacgccg cgcgtgggcg gcgtgggccc
     1021 caccacgatt gccctgttgt tccgcaacgc cgtcgaagcc gccgagtccc gggcccgcct
     1081 gggctgagcg ccccggtcga ccgctgggcc gctgggccgt gcgggtgccc ttgcgcgtcg
     1141 aatcgacgtc gattcgacgc gcaaggggca cgctggggac cgccggtcca ggagggacgg
     1201 cgggccgaac cgggcgtggg ccgtgaacgg ctggtggcct agcctcagtg gaccatgacc
     1261 aggatctccg acctcctcgc caagggcccc acctggtcct tcgagttctt cccgccgaag
     1321 acgcccgagg gcatgacctc cttcgaccgc gcggtcaccg acctgtcggc gctcgagccg
     1381 gcctacgtgt cgatcaccta cggcgctggc ggttctgctc gcgacaccac ctacgacctc
     1441 gtggtccggg tgaacgagga gcaggcgttc cccgccatgc cccacctgac ctgcgtcggc
     1501 cacacccggg ctgagctcat cgacctgctc gagtcctacc gggccaacgg cgtcgacaac
     1561 gtgctcgccc tggccggtga tccgccgaag gacgggtccg accccggcgg cgacttcgtg
     1621 tacgccaccg agctggtcgc cctcgcccgg gaggtcggcg acttctccgt cggcgtggcc
     1681 gcgttccccg aggtccaccc ccggtccacc gaccgggcca cggaccgccg gttcctcgcc
     1741 gccaaactga acgccgccga cttcggcatg acccagttct tctgggatgc cgaccactac
     1801 cgggtgatgg tcgaggaact cgcggcgctg ggctgcacca caccggtgct gccgggcgtc
     1861 atgccgttcg tgagcgtcgc cggcgcccgc cggatgtctg ggatgaacaa cacggacatc
     1921 cctgcctggc tgcaggagcg gatggacgag gtcgacggcg acgccgaggc cacgcgccga
     1981 ctcggcgtcg aggtcgccac cgggctcatc gagcagctgc tcgaggtcgg ggtgcccggc
     2041 atccacctgt acgccatgaa ccgggcccag tcgatccagg agatctacga caacctcggc
     2101 ctccgccgct gatccgctga ccccggcggt cgagcagatg cggcgacggc gccgacgtca
     2161 ggccagcagg gcgatcgccg cgttgagggt ggggctgggg cgcatggcgg ccgacacctt
     2221 ggcgtcgtcg ggcgagtagt acccgcccat gtcgaccggg ctgccctgga cgccgttgag
     2281 ctcagccatg atcgccgctt cgccgtcgac cagcgcggcc gcgaccggag cgaacctcgc
     2341 agcgatctcg gtatcggcgg cctgggcagc cagctcctcg gcccagtagc gggccaggta
     2401 gtagtggctg ccgcggttgt cgatctcgcc ggccttgcga gagggcgagc ggccctcgtc
     2461 gaggaagcgg ccggtggcag cgtcgagcgc gtcggccagg atctgggccc gcgggttggc
     2521 ggcgaactcg gcaaggtgct caagcgacgc ctggagcgcc aggaactcgc cgagggagtc
     2581 ccagcggagg tggttctcct tctcgaactg ctggacgtgc ttgggg
//
LOCUS       DATMFS010000375         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_292314, whole genome shotgun sequence.
ACCESSION   DATMFS010000375 DATMFS010000000
VERSION     DATMFS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292314"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VNQ33_06935"
     CDS             complement(<1..591)
                     /locus_tag="VNQ33_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97878.1"
                     /translation="MTTTVVDGVEITTSQEERVTQAPSDVLRLAVAVVALLVVTLLGA
                     LFGQAMVGFMADLLRGLDQLPTWFLTALAVAAQIVGVVLFSAGLAAVVRSRAWHVLIA
                     AIVAAVGAGVLTAVLRKVTADPGAQVADAHTIAAIGGGGSWSAGAVAALVAVVTASAP
                     WVPRPWRRVAWGLTMAVALVHFVESPVAFDTLLAILA"
     gene            complement(591..2018)
                     /locus_tag="VNQ33_06940"
     CDS             complement(591..2018)
                     /locus_tag="VNQ33_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HWJ97879.1"
                     /translation="MPTSANNVPHFGVPHPTVEERVAIGKAARKVVPRSAHAVWSESQ
                     RTHDPIALLQQQAETRVPDLIPIRHGRMSATAFANYRGAALMMAADLATVPDSGLTVQ
                     LCGDAHLSNFGGFASPERKLVFDLNDFDETLPGPFEWDVKRLVASIEVAARSNGFDDA
                     TRADMVLAAGRSYREAMAQFAVMRDLEVWYSSIDVETTLAAIQDQADAAQRKRTKKGL
                     AKAHNKNQLRAAAKLTTVVDGHLRFIDDPPLVVSAGKLFGTELGKDLEQAVRGSLDSY
                     HESLNEDRRHLLDKYQFVDLARKVVGVGSVGTRAWIALFVGRDDDDPLVLQVKEAQAS
                     VLEQFLPASRYATHGQRVVEGQRLMQASSDILLGWQQTDGIDGVRRDYFMRQLWDWKA
                     SVEVDLLEPPMLSRYGELCAWTLARAHARSGDRIAISAYLGTSDGFDRAMAEFAAAYA
                     DQNDRDHAALLAAIADGRLEAETGI"
     gene            2252..2692
                     /locus_tag="VNQ33_06945"
     CDS             2252..2692
                     /locus_tag="VNQ33_06945"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HWJ97880.1"
                     /translation="MTDEDVHGPIDFVLIEFQGDHLTGEAGAALLDLVDAGTIRIWDL
                     LVVTKDTDGTVVGIDIEDLDADHLGSFTAFSGAASGLLGEDDVFEAGNALEPGTVAAL
                     IVYENTWAIPFVKAARNAGAEVVAAARIPADTVIEVLEAAEAAS"
     gene            2707..>3019
                     /locus_tag="VNQ33_06950"
     CDS             2707..>3019
                     /locus_tag="VNQ33_06950"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWJ97881.1"
                     /translation="MPRLLRGVARTAVIAGTATAVSGRVQRRQAEKYTDRDAQIYADR
                     TTAYQQDVQAQQAQAAPPPPPPAAAPAPPAGGDMIEQLKQLGELHAQGILTDEEFAAQ
                     KA"
BASE COUNT          469 a         1098 c         1053 g          399 t
ORIGIN      
        1 ggcgaggatg gcgagcaacg tgtcgaaggc gaccggcgat tcgacgaagt gcacgagcgc
       61 caccgccatc gtgaggcccc aggcgacgcg gcgccacggg cgcggcaccc agggcgccga
      121 cgccgtgacg accgcgacga gcgcggcgac ggcccccgcc gaccacgagc cgcccccgcc
      181 gatggcggcg atggtgtggg cgtcggccac ctgggctccc gggtcggcgg tgaccttccg
      241 gagcacggcc gtgaggacac ccgcgccgac ggccgcgacg atcgcggcga tgaggacgtg
      301 ccacgcacgg gaccgcacga cggcagcgag gccagccgag aacagcacca cacccacgat
      361 ctgggcggcc accgccaggg cggtcaggaa ccaggtcggg agctggtcga gcccgcgcag
      421 caggtcggcc atgaagccga ccatcgcctg gccgaagagc gcgccgagga gcgtcaccac
      481 gagcagggcg acgacggcca ccgccaggcg gaggacgtcg ctcggcgcct gggtgacacg
      541 ctcctcctgg ctcgtggtga tctccacccc gtccaccacc gtcgtcgtca tcagatcccg
      601 gtctccgcct cgaggcggcc gtcggcgatg gcggcgagca aggcggcgtg gtcgcggtcg
      661 ttctggtcgg cgtaggcggc ggcgaactcc gccatcgccc gatcgaagcc gtcgctggtg
      721 cccaggtagg cggagatggc gatgcggtcg cccgagcggg cgtgggcccg ggcgagggtc
      781 caggcgcaca gctcgccgta gcgcgagagc atggggggct cgagcaggtc gacttcgacc
      841 gacgccttcc agtcccagag ctggcgcatg aagtagtcgc gccggacgcc gtcgatcccg
      901 tcggtctgct gccagccgag gaggatgtcg ctcgaggcct gcatgagccg ctggccctcg
      961 accacgcgct ggccgtgcgt cgcgtaccgc gatgccggga ggaactgctc gagcaccgaa
     1021 gcctgcgcct ccttcacctg gagcaccagc ggatcgtcgt cgtctcgccc cacgaacagg
     1081 gcgatccacg cacgggtccc gacgctgccg acaccgacga ccttccgggc caggtccacg
     1141 aactggtact tgtcgagcag gtgtcggcgg tcctcgttga ggctctcgtg gtacgagtcg
     1201 agcgacccgc ggaccgcctg ctccaggtcc ttgccgagct cggtgccgaa cagcttcccg
     1261 gccgacacga cgagcggcgg gtcgtcgatg aaccgcagat ggccgtcgac gaccgtcgtg
     1321 agcttggccg ccgcccgcag ctggttcttg ttgtgcgcct tggccagccc cttcttcgtc
     1381 cgcttgcgct gggcggcgtc ggcctggtcc tggatggcgg ccagcgtcgt ctcgacgtcg
     1441 atcgacgagt accagacctc gaggtcgcgc atcaccgcga actgggccat cgcctcgcgg
     1501 tacgagcggc cggcggcgag caccatgtcg gcgcgggtcg catcgtcgaa cccgttggac
     1561 cgagccgcca cctcgatgct ggcgacgagg cgcttcacgt cccactcgaa cgggccgggc
     1621 agcgtctcgt cgaagtcgtt caggtcgaag accagcttgc gctcgggcga ggcgaacccg
     1681 ccgaagttgc tgaggtgcgc atcgccgcag agctggacgg tgagcccgct gtcggggacg
     1741 gtggccagat cggcggccat catcagcgcc gcgccgcggt agttggcgaa cgccgtcgcc
     1801 gacatgcgcc cgtggcggat cgggatcagg tccgggaccc gggtctccgc ctgctgctgg
     1861 agcagggcga tcgggtcgtg ggtccgctgg gactcgctcc acacggcgtg ggcgctgcgg
     1921 ggcacgacct tgcgcgccgc cttgccgatg gcgacacgct cctcgacggt ggggtgcggt
     1981 acgccgaagt gcgggacgtt gttggcagac gtgggcatgg ggtgggctcc gggggcgtcg
     2041 gcgcgggccg gctgcgatcg cccacccgca tgcgtgatcg cacccgttct taccaggctg
     2101 ctccggttgt ggccgcgtga acacagcgcg cgtgcgaaca tggccccgtc cgaccgccag
     2161 gctgcggccg aacgagcggt cgctttggta agcatgcccc gctcacaacc accaccccgc
     2221 cccggggcgc accccaaccg aggacatcac agtgaccgac gaagacgtac acggaccgat
     2281 cgatttcgtc ctgatcgagt tccagggcga ccacctcacc ggtgaggccg gcgccgcgct
     2341 gctcgacctg gtcgatgccg gcaccatccg catctgggac ctgctggtcg tcaccaagga
     2401 caccgacggg accgtcgtcg gcatcgacat cgaggatctc gacgcagacc acctcggcag
     2461 cttcaccgcc ttctccggcg ccgccagcgg gctgctcggc gaagacgacg tgttcgaggc
     2521 cggcaacgcc ctcgaacccg gcaccgtcgc cgcgctcatc gtctacgaga acacctgggc
     2581 gatcccgttc gtgaaggccg cccgcaacgc cggcgccgag gtcgtggccg ccgcccgcat
     2641 ccccgccgat accgtcatcg aagtgctcga agccgctgaa gcggccagct gagaggaacc
     2701 agacccatgc cacgattgct ccgaggagtc gctcgcaccg ccgtcatcgc cggcaccgcc
     2761 accgcggtga gcggccgggt ccagcgccgt caggccgaga agtacaccga ccgcgatgct
     2821 cagatctacg ccgaccgcac caccgcctac cagcaagacg tgcaggccca gcaggcgcag
     2881 gccgcaccgc caccacctcc gcccgctgcc gcccccgccc ctcccgccgg tggcgacatg
     2941 atcgaacagc tcaagcagct gggcgagctg cacgcccagg gcatcctcac cgacgaggag
     3001 ttcgccgccc agaaggcca
//
LOCUS       DATMFS010000376         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_292411, whole genome shotgun sequence.
ACCESSION   DATMFS010000376 DATMFS010000000
VERSION     DATMFS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292411"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1378
                     /locus_tag="VNQ33_06955"
     CDS             182..1378
                     /locus_tag="VNQ33_06955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ97882.1"
                     /translation="MDSAPRVGTDDDRLGLWEVFRLGFGFSDRQRDDWLGFLDPSRAL
                     IVDGPRGEVAAASHIRRFDQWFGGRAVPLAGFSPVAVLPEFRGRGLARTVVAGQYDDL
                     RARGEVMSGLFPASLALYRSVGFELAGSYVLRRFPAAEVGRLRPRRDVEVRRGTVDDV
                     AAVHRRHEAVGPAHDGQLRRDPAWWSRRLPTDLDGVVLYVVDDPGRPGELAGYAIYRH
                     GPAPKPYEYSVVVTEVVADDPDVLRALWRVVGSSGSQAPWIEVIGPAEDDLFLLLANA
                     DPVAVRNEIRWMVRIIDLPGAVAARGWPADARGTVEIDVVDDDAPWNAGRWTLEVADG
                     AATARAGGAGLVQATIGGLSSWWAGYAPATKLARLGLLHADDHRTLARMDALLPAVPP
                     VLPDFY"
     gene            complement(1394..1600)
                     /locus_tag="VNQ33_06960"
     CDS             complement(1394..1600)
                     /locus_tag="VNQ33_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97883.1"
                     /translation="MAGEFDEIRGRLEAIAEELADLAIVRLRESIDAGGTEYPVDEKR
                     LTRARRAVEKAVNLLSEPDEHAYD"
     gene            complement(1633..>2137)
                     /locus_tag="VNQ33_06965"
     CDS             complement(1633..>2137)
                     /locus_tag="VNQ33_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWJ97884.1"
                     /translation="GTLDETLELLTLQQTGKAEPTPIVLLDAPGGTFWQGFASFVQDE
                     VAQRGLVAADDLDRVLITDSWEAAVAEILGFWRNYHSLRWVGKRLVLRLRAEPTASEA
                     AALNEQFGDRLLEGGIERRGPLAAEVSSDDHVGLARLVMVWNPFKVGDLHQLIRAVNA
                     LPSAPAS"
BASE COUNT          264 a          782 c          774 g          317 t
ORIGIN      
        1 cagttgctgg tgaggaacgt cacgtcgaag atccacgtcc gatcctcggt cggatcctcg
       61 aacgacaccc actcgtggag gtcagccaac tcggtcaccg agcgaagcta cccgcggcgt
      121 cgtcggtccc ggaacccgaa acgggacgtg ggccgaccag cggtgcgcct acggtcacgc
      181 catggactca gcgccgcgcg tgggcaccga cgatgaccgg ctcggcctct gggaggtctt
      241 ccgcttgggg ttcgggttca gcgatcggca gcgggatgac tggctgggct tcctcgaccc
      301 gtcgcgtgcg ctcatcgtcg acgggccgcg gggggaggtc gccgccgcgt cccacatccg
      361 gcggttcgac cagtggttcg gcggtcgagc ggttccactc gccgggttct ccccggtcgc
      421 cgtcctcccg gagttccgag gccgagggct ggcccgcacc gtcgtcgccg gccagtacga
      481 cgacctccga gcgcgcggcg aggtgatgtc gggcctgttc cccgcatcgc tcgccctgta
      541 ccgatcggtc gggttcgagc tggccggctc ctacgtgctc cgccggttcc cggccgccga
      601 ggtcggccgc ctgcgtccgc gccgagacgt cgaggtccga cgggggaccg tcgacgacgt
      661 tgccgccgtc caccgccgcc acgaggcggt cggccccgcg cacgacggcc agctgcggcg
      721 cgacccggcc tggtggtccc gccgcctgcc caccgacttg gacggtgtcg tgctctacgt
      781 ggtcgacgac cccggtcggc ccggtgagct ggccggctac gcgatctacc gccacggccc
      841 cgcgccgaag ccctacgagt acagcgtcgt ggtcaccgag gtggtggccg acgaccccga
      901 cgtcctccgg gcactgtggc gcgtcgtcgg cagctccggc tcgcaggcgc cgtggatcga
      961 ggtgatcgga ccggcggagg acgacctctt cctcctgctg gccaacgccg accccgtggc
     1021 cgttcgcaac gagatccggt ggatggtgcg gatcatcgac ctgcccggcg ccgtcgcggc
     1081 gcggggttgg ccggccgacg cccgggggac cgtcgagatc gacgtcgtcg acgacgatgc
     1141 gccctggaac gccggacgct ggacgctgga ggtggccgac ggcgccgcga cggcccgcgc
     1201 cggcggcgcc ggtctcgtgc aggcgaccat cggcgggctg tcctcgtggt gggcgggcta
     1261 cgcgccggcc accaagctcg cccgcctcgg tctcctgcac gccgacgacc accggaccct
     1321 cgcccggatg gacgcgctcc tccccgcggt ccctccggtc ctgcccgact tctactgagg
     1381 cgtcggtccg ccgtcagtcg tacgcgtgct cgtcgggctc gctgaggagg ttgacggcct
     1441 tctcgacggc tcggcgggcg cgggtgaggc gcttctcgtc caccgggtac tcggtgccgc
     1501 cggcgtcgat ggactcccgg aggcgcacga tggccagatc ggccagctcc tcggcgatgg
     1561 cctccagccg gccccggatc tcgtcgaact ctcctgccat ggccgccgag tctgccaggc
     1621 gggtctgaca gctcaggagg ccggcgcgct cggcagggcg ttgacggccc ggatcagctg
     1681 gtggagatcg ccgaccttga acgggttcca caccatgacg aggcgggcca ggccgacgtg
     1741 gtcgtcgctc gacacctcgg cggcgagcgg tccgcgccgc tcgatcccgc cttcgaggag
     1801 gcggtcgccg aactgctcgt tcagcgcggc cgcctccgaa gcggtcggct cggcgcggag
     1861 tcgcagcacg aggcgcttgc ccacccagcg gagcgagtgg tagttccgcc agaagccgag
     1921 gatctcggcg acggcggcct cccagctgtc ggtgatcagc acgcggtcga ggtcatcggc
     1981 ggccaccagg ccgcgctgcg ccacctcgtc ctgcacgaac gaggcgaacc cctgccagaa
     2041 cgtgccgccg ggcgcatcga gcaagacgat gggggtcggc tcggccttgc cggtctgctg
     2101 gagggtgagc agctcgagcg tctcgtcgag cgtgccg
//
LOCUS       DATMFS010000377         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_59264, whole genome shotgun sequence.
ACCESSION   DATMFS010000377 DATMFS010000000
VERSION     DATMFS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59264"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1193
                     /gene="tuf"
                     /locus_tag="VNQ33_06970"
     CDS             6..1193
                     /gene="tuf"
                     /locus_tag="VNQ33_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWJ97885.1"
                     /translation="MGKEKFERNKPHLNIGTMGHIDHGKTTLTAAITKTLHDADPTTA
                     FTPFDMIDKAPEERQRGITINISHVEYETANRHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVAATDGPMPQTREHVLLARQVGVPSIVVALNKCDMVDDEELLELVEMEVRELLT
                     EYEFPGDDTPVVRVSALKALEGDADAAAQVLELMAQVDAFVPEPVRDIDKPFLMPIED
                     VFSITGRGTVVTGKVEAGQIHTGDEVEIVGLKDTQRTTCTGVEMFRKLLDEGQAGDNI
                     GALLRGTKKEEVERGQVLCKPGSITPHTNFVGEVYVLTKEEGGRHKPFFANYRPQFFF
                     RTTDVTGTISLPEGTEMCMPGDNTSMTVELIAPIAMDEGLRFAIREGGRTVGAGRVTE
                     ILK"
     gene            complement(1422..>2338)
                     /locus_tag="VNQ33_06975"
     CDS             complement(1422..>2338)
                     /locus_tag="VNQ33_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ97886.1"
                     /translation="SASASSPVGPSPDRAAAPTAGAPRPAVAPAPEEAAGPGARAPKI
                     RLDHLYRYFGRARTGRPGPPVVRAVDDVSLDLPAGTVTALVGESGSGKTTLARIVAGA
                     DTATAGEVLIDGRVLSPDRTGDERRTVQLVSQNHRAALNHRRSVAHALVQVQRVHGIG
                     TDRTDRTDRAAALLDRVGLTRDHLRRRPRQLSGGEAARVALARSLLLEPEVLVLDEPT
                     SGLDARVRSAVIELLADLRHELGLTVLLITHELPVADALADHVAVMHDGELVEHGPAD
                     QVLHQPIDGYTKALLASVPPDPRTLAAP"
BASE COUNT          362 a          668 c          941 g          367 t
ORIGIN      
        1 cggtcatggg caaggaaaag ttcgagcgga acaagccgca cctgaacatt ggcacgatgg
       61 gtcacatcga ccatggcaag acgacgttga cggcggcgat cacgaagacg ctgcatgatg
      121 cggatccgac gacggcgttc acgccgttcg acatgatcga caaggcgccg gaggagcgtc
      181 agcgggggat cacgatcaac atctcgcatg tggagtatga gacggcgaac cgtcactacg
      241 cgcatgtgga tatgccgggt cacgcggatt acatcaagaa catgatcacg ggtgcggcgc
      301 aggtcgatgg ggcgattctg gtggtggcgg cgacggatgg tccgatgccc cagacgcgtg
      361 agcatgtgct gttggcgcgt caggtggggg tgccgtcgat cgtggtggcg ctgaacaagt
      421 gcgacatggt cgatgacgag gagctgttgg agctggtgga gatggaggtc cgggagctgc
      481 tgacggagta cgagttccct ggtgacgata cgccggtggt gcgggtgtcg gcgctgaagg
      541 cgctggaggg cgatgcggat gcggcggcgc aggtgctgga gctgatggcg caggtggatg
      601 cgttcgtgcc ggagccggtg cgtgatatcg acaagccgtt cttgatgccg atcgaggatg
      661 tgttctcgat cacggggcgt gggacggttg tgacggggaa ggtggaggcg ggtcagatcc
      721 acacgggtga cgaggtggag atcgtggggc tgaaggacac gcagcgcacg acgtgcacgg
      781 gtgtggagat gttccgcaag ctgctcgacg agggccaggc gggggacaac atcggcgctt
      841 tgctgcgggg gacgaagaag gaggaggtgg agcggggtca ggtgctgtgc aagccgggca
      901 gtatcacgcc gcacacgaac ttcgtgggtg aggtgtatgt gctgacgaag gaggagggcg
      961 gtcgtcacaa gccgttcttc gcgaactacc gtccgcagtt cttcttccgg acgactgatg
     1021 tgacggggac gatcagtctg ccggagggga cggagatgtg catgccgggt gacaacacgt
     1081 cgatgacggt ggagctgatc gctccgatcg cgatggatga gggactgcgg ttcgccatcc
     1141 gtgagggcgg tcgcaccgtg ggcgccggcc gcgtcaccga gatcctcaag tagtcctcgc
     1201 cgggctcgcc ccggtcggaa cgaacgtcag cagggccgcc ctccggggcg gcccttcttc
     1261 gtgctgcgat gcgcctgacc gggaccccgt gaaccttcgc aggcggacat cgcccggacc
     1321 cgagccgagg ttggtgtggt ttctgctgct ctgcctcaga aaccacacca acctcggtcg
     1381 ggccaggaag gtggggtggc caggacgggg cgttggggtg gtcagggcgc tgcgagggtc
     1441 ctggggtcgg gtgggacgct ggcgaggagg gccttggtgt agccgtcgat gggctggtgg
     1501 aggacctggt cggcggggcc gtgctcgacc agctcgccgt cgtgcatcac ggcgacgtgg
     1561 tcggcgaggg cgtcggccac cggcagctcg tgggtgatga gcagcacggt caggcccagc
     1621 tcgtgccgga ggtcggccag cagctcgatc accgcggagc ggacgcgggc gtcgaggccg
     1681 gacgtcggct cgtcgaggac cagcacctcg ggctcgagca gcagcgagcg ggcgagcgcc
     1741 acgcgtgcgg cctcgccgcc gctcagctgg cgaggccggc gcctcaggtg gtcgcgggtg
     1801 aggcccaccc gatcgagcag cgccgcggcc cggtccgttc ggtcggtgcg gtcggtgccg
     1861 atcccgtgca cccgctgcac ctggacgagc gcgtgggcca cgctgcgacg gtggttgagg
     1921 gcggcccggt ggttctggct gacgagctgg accgtgcgcc gttcgtcacc ggtgcggtcg
     1981 ggtgagagga cccggccgtc gatcaggacc tccccggccg tggcggtgtc ggccccggcc
     2041 acgatgcggg ccagcgtcgt cttccccgag cccgattcgc cgaccagcgc ggtgacggtg
     2101 ccggctggga gatcgaggct cacgtcgtcg accgcccgca ccacaggtgg tcccggccgg
     2161 ccggtgcgag cgcggccgaa gtagcggtac aggtggtcca ggcggatctt cggggctcga
     2221 gcgccgggac ctgcggcttc ctccggtgcc ggcgcgaccg ccggtcgtgg cgcgccggcg
     2281 gtcggtgccg cggcccggtc cggcgacggt ccgaccggac tggaggccga agcggagg
//
LOCUS       DATMFS010000378         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_244622, whole genome shotgun sequence.
ACCESSION   DATMFS010000378 DATMFS010000000
VERSION     DATMFS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244622"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VNQ33_06980"
     CDS             <1..868
                     /locus_tag="VNQ33_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HWJ97887.1"
                     /translation="GEPPRFHPVAGFGTAMGAVERRLWRDDRRAGIAYTAAGLALGAG
                     AGTAVRSTTAAVALGSAGRELRRTAAAIGERCAADDLDGARAALPTLVGRDPSELDHQ
                     GVAAAVIESLAENSVDAVVAPALWGAAAGAAGAAGYRAVNTMDAMVGHRSERYERFGW
                     SAARLDDAANLVPARVFALLVVAVRPARARSIAAAIRTQAGAHPSPNAGIAEAAVAAA
                     LGAELGGTLRYGDRVEERPRLGSGPRPQPADIGRAIALVDQVELLLTALLVAPAAIGA
                     ARRRWDQRRRRP"
     gene            868..1785
                     /locus_tag="VNQ33_06985"
     CDS             868..1785
                     /locus_tag="VNQ33_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ97888.1"
                     /translation="MSCEPAPHGGDAPAVAQRLGIDRRALLDLSASLNPVAPDVRPIV
                     ARHLDALQDYPDDRRATAALAEAMGVDADRLVLTNGGAEAIALVARLHPRGWADACDF
                     SLYRRHLIDLDPTGPRWMSDPNNPTGTLAGPGDRAFVRDEAFYPLATGRWTRGDADAI
                     VLGSLTKAFACPGLRIGYVLAPDAETARRVAAVRPRWSVSGLACDALPDLLAVADLAA
                     WRSGIAELRGQLSNLLAAHGISAAPGAANYLWVPDAGDLRAHLFAHQILIRSGASFGH
                     PDAVRIAVPTTTGLERLARALDSYEVPDA"
     gene            1778..2812
                     /gene="cobT"
                     /locus_tag="VNQ33_06990"
     CDS             1778..2812
                     /gene="cobT"
                     /locus_tag="VNQ33_06990"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWJ97889.1"
                     /translation="MPDRPILPAIAPLDTGAMAAAQERWASRAKPPGSLGQLEDLAVQ
                     LAGITGTCPPPVITRPAIAVFAGDHGVVADGASAWPSDITGLMVRAMAGEVAAINTFA
                     ATVGADVHLVDVGVASDLGGLPNVRHRKVRAGTASLAHGPAMSVGEAGAALAVGLDVA
                     AELIAGGADCLVAGDMGIGNTTPSAALIGRFAGAMADTVTGTGAGLPADRLDAKTAIV
                     ADAIERAGQIDDPLAVLAEIGGLEIAAMAGLYLGAAAAAVPFIVDGVIAAAAALTADA
                     LVPGTAARAVAGHQSTEPAAAVALKHLGLEPLLDLRLRLGEGTGACLAIPLLQASARA
                     LRDMADLPEG"
     gene            complement(2828..>3848)
                     /locus_tag="VNQ33_06995"
     CDS             complement(2828..>3848)
                     /locus_tag="VNQ33_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWJ97890.1"
                     /translation="AVEARIETWRRTGQLTEDDRSWQELVAAAVLDLIADGSVSSRRG
                     QARPLLIVTAKLNDLFDRAGVPAEQRAAWSARIVGGGPIGQAALRELIEQANLQLVVT
                     DDDGETLFIGRAKRLATAAMLVALIARSGGTCEFPGCHARHHRAHAHHVVWWEHGGCT
                     DIDNLALLCPHHHRLVHRGLDPHPRTRRPRLPKARRHHHRTAPVPASRLSTPPGPAAR
                     PDPWGGSAGAHQAGGGGGRSGERTTTAGRAHTHDHARPARAWSPCPWVRWAAGVPAPQ
                     RPSVGSAGCCGRRRGSDVPVVLAHQGGWDEALMVLAVICLFVGLLWIANARAGAQSHG
                     DDAST"
BASE COUNT          428 a         1564 c         1377 g          479 t
ORIGIN      
        1 cggcgagccg ccgcggttcc acccggtcgc cggcttcggt acggccatgg gcgcggtcga
       61 gcggcggctc tggcgcgacg accggcgggc cggcatcgcc tacaccgccg ccgggctggc
      121 cctcggggcc ggggccggta ccgccgtccg ctcgacgacg gccgccgtcg ccctcggctc
      181 ggccggccgg gagctgcggc ggacagccgc ggcgatcggc gagcggtgcg cggccgacga
      241 cctcgacggg gcccgggcgg ccctgcccac gctggtgggt cgggacccga gcgagctcga
      301 tcaccagggc gtcgccgccg ccgtcatcga gtcgctcgcc gagaactcgg tcgatgccgt
      361 ggtggccccc gccctgtggg gcgcggcggc cggcgcggcg ggcgccgccg ggtaccgggc
      421 cgtcaacacc atggacgcca tggtcgggca ccgcagcgag cggtacgagc gcttcggatg
      481 gtccgccgcc cggctcgacg acgccgccaa cctcgtcccg gcccgggtgt tcgccctcct
      541 cgtcgtcgcc gtccgaccgg cccgcgctcg ttcgatcgct gccgcgatcc gcacccaggc
      601 cggcgcccac ccgtccccga acgccgggat cgccgaggcc gcggtggcgg ccgcgctggg
      661 cgccgagctg ggcggcacgc tccgctacgg cgaccgggtc gaggagcgac cccggctcgg
      721 atccggccca cggccgcagc ctgccgatat cggccgggcc atcgcgctgg tcgaccaggt
      781 cgagctgctg ctcacggccc tcctcgtcgc gccggcggcg atcggggccg cccggcgccg
      841 gtgggaccag cggaggcgcc ggccgtgatg tcctgcgaac ccgcacccca cggaggagac
      901 gcccccgccg tagctcagcg gctcggcatc gaccgccgcg ccctgctgga cctctcggcc
      961 agcctcaacc cggtcgcccc cgacgtgcgg ccgatcgtcg cccggcacct cgacgccctc
     1021 caggactacc ccgacgaccg gagggccacc gcggcgctcg ccgaggcgat gggcgtcgat
     1081 gccgaccgcc tcgtcttgac caacggcggc gccgaggcga tcgccctcgt cgcccggctg
     1141 cacccgcggg gctgggccga cgcctgcgac ttctcgctgt accggcggca cctgatcgac
     1201 ctcgatccca ccggaccgcg gtggatgtcg gacccgaaca accccaccgg caccctcgcc
     1261 ggcccaggcg accgggcgtt cgtccgtgac gaagccttct accccctcgc caccggccgc
     1321 tggacccggg gcgacgcgga cgccatcgtg ctcggctcgc tcaccaaggc gttcgcctgc
     1381 cccggcctgc ggatcgggta cgtcctcgcc cccgacgccg agaccgcgcg acgggtggcc
     1441 gccgtccgcc cgcgctggtc cgtgagcggc ctggcctgcg acgcgctccc cgacctgctc
     1501 gccgtcgccg acctggcggc ctggcggtcg ggcatcgccg agctccgggg gcagctgtcg
     1561 aacctgctcg ccgcccacgg gatcagcgcc gcgcccggag ccgccaacta cctctgggtc
     1621 cccgacgccg gtgacctccg agcccacctg ttcgcccacc agatcctgat ccgctccggc
     1681 gccagcttcg gccaccccga cgccgtccgg atcgccgtcc ccaccaccac cgggctcgag
     1741 cgcctcgctc gcgccctcga ctcctacgag gtccccgatg cctgaccgtc ccatccttcc
     1801 cgccatcgcc ccgctcgaca ccggcgccat ggccgccgcc caggagcgct gggcgtcgcg
     1861 ggccaagccg cccggctccc tcggccagct cgaggacctc gccgtccagc tggcgggcat
     1921 caccggcacc tgcccgccgc ccgtcatcac ccgcccggcc atcgccgtgt tcgctggcga
     1981 ccacggtgtg gtggccgacg gggccagcgc ctggccgtcg gacatcaccg gactgatggt
     2041 ccgggccatg gccggtgagg tggcagccat caacaccttc gccgccaccg tcggcgccga
     2101 cgtccacctc gtcgacgtcg gcgtcgccag cgacctcggc ggcctcccca acgtccgcca
     2161 ccgcaaggtc cgagcgggca cggcgagcct ggcgcacggg ccggccatgt cggtcggcga
     2221 ggccggcgcg gcgctcgccg tcggcctcga cgtcgccgcc gagctcatcg ccggcggcgc
     2281 cgactgcctc gtcgccggcg acatgggcat cggcaacacc accccctcgg ccgccctcat
     2341 cggccgcttc gccggcgcca tggccgacac ggtcaccggc acgggcgccg gcctccctgc
     2401 cgaccgcctc gacgccaaga cggcgatcgt cgccgacgcc atcgagcggg cgggccagat
     2461 cgacgacccg ctcgccgtcc tcgccgagat cggcggcctg gagatcgcgg cgatggccgg
     2521 cctctacctg ggcgccgccg ccgccgcggt cccgttcatc gtcgacgggg tcatcgccgc
     2581 cgccgccgcc ctcaccgccg atgccctcgt ccccggcacg gccgcccggg ccgtcgccgg
     2641 ccaccagtcg acggagccgg ccgccgcggt ggcgctgaag cacctcggcc tcgagccgct
     2701 gctcgacctg cgcctccgcc tcggcgaagg caccggcgcc tgcctggcga tcccgctgct
     2761 ccaggcgtcc gcccgcgccc tgcgggacat ggctgacctg cccgagggct gatcgggccg
     2821 ggctgggtca ggtcgaggcg tcgtcgccgt ggctctgggc gccggcccgg gcgttggcga
     2881 tccagaggag cccgacgaac aggcagatca cggccagcac catcagcgcc tcgtcccatc
     2941 caccctggtg ggccagcaca accggcacgt cgctccctcg ccttcgtccg caacagcccg
     3001 ccgatcctac cgagggtcgt tgcggcgcgg gaacgcccgc tgcccaccgg acccacgggc
     3061 acggcgacca cgcccgcgcg ggacgggcgt ggtcgtgggt gtgcgcccgt cccgcggtgg
     3121 tcgtgcgctc tccggatctc ccccctcctc ctcccgcctg gtgggcacct gctgatccac
     3181 cccacggatc cggccgtgct gccggtcctg gtggggtgct caggcggctt gctggaacgg
     3241 gggcggttcg atggtggtgc cgtcgggcct tcggaagacg aggccggcgg gtccgcggat
     3301 gagggtccag gccccggtgc acgagccggt ggtggtgcgg gcacaggagg gcgaggttgt
     3361 cgatgtcggt gcatccgccg tgctcccacc acacgacgtg gtgggcgtgg gcgcggtggt
     3421 ggcgggcgtg gcagccgggg aactcgcagg tgccgccgga gcgggcgatg agggcgacga
     3481 gcatggcggc ggtcgcgagc cgcttggccc ggccgatgaa gagggtttcg ccgtcgtcgt
     3541 cggtgacgac gagctggagg ttggcctgct cgatcagctc ccgaagggcg gcttggccga
     3601 tgggaccgcc gccgacgatc cgagcggacc acgcggcgcg ctgctcggcg gggacgccgg
     3661 cgcggtcgaa caggtcgttc agcttggcgg tgacgatgag cagcgggcgg gcctggccgc
     3721 gccgcgacga cacggacccg tcggcgatca ggtcgagcac cgccgccgcg accagttcct
     3781 gccagctgcg gtcgtcctcg gtgagctgcc cggtgcgccg ccaggtctcg atgcgcgcct
     3841 ccaccgcc
//
LOCUS       DATMFS010000379         4839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_292579, whole genome shotgun sequence.
ACCESSION   DATMFS010000379 DATMFS010000000
VERSION     DATMFS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292579"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VNQ33_07000"
     CDS             <1..840
                     /locus_tag="VNQ33_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97891.1"
                     /translation="EGVGGATPYTLTDSQGRFIKSVTWQGPALNTVFMRYSRDEVRYI
                     LTYGRPFSPMPAWGTAGGGPLTEQKLQELMDYISTFQLTPAESQKAVTAQLEVMMKKK
                     DQTCVDAKTDEAKSALPEGETFDETKVDTSDCPPMYKSEGDALFNMGYDDGFAGGAYS
                     CGRCHTKGWSYGEKAADGSGAFGPPLSNVLQQFPGATLGPKDQTDFVCTGSDQGVRYG
                     QTGQGTGRMPGFCITPGYKINPLNGEVGIEPFDTGTAEQGAMFTQDQVRKIVEYERSL
                     ARD"
     gene            833..1783
                     /locus_tag="VNQ33_07005"
     CDS             833..1783
                     /locus_tag="VNQ33_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97892.1"
                     /translation="MTSLLAALAWDPGFRGILTVAVAVAILCGSVFLILATNNGARLG
                     FLLALTGFCGWMFVLGTIWSMYGIGWKGSAPTWKVVDVVRSEPGSGEIVSSLEAAESL
                     PLPDELPDAVELRDSSKVFAENFPATQRDPKVGDLVSIDDPEAKELKEKINAQVAPWR
                     ILETSNKYTGETQAFVSTALGPDGQAIFESASDYVVLDSFLVGGKKARTDNSTIGRVR
                     WKLGSIVNQSPPDLYAAVQLQAVIPQETKDGQAPPTPVANPKADIITVVLKRIDGHHL
                     RRNAIGMTIFMGAATAVLCSMLHRRDKLAQVQRAQTAGAA"
     gene            1786..2262
                     /gene="ndhC"
                     /locus_tag="VNQ33_07010"
     CDS             1786..2262
                     /gene="ndhC"
                     /locus_tag="VNQ33_07010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWJ97893.1"
                     /translation="MGQYLPILVMLVLGVLFAGVSLVMSQLVSPKRPSAAKSAPYECG
                     IVPRQEPPERFPVRFYLVAMIFVVVDIEIIFLYPYVVIHRELGVFGLIEMLVFAIAVF
                     ASFVYLIAHGALDWGPIAQLRRSSNPAVSTERTAASTVRRVGQEGRGPKAPSEVAA"
     gene            2262..2840
                     /locus_tag="VNQ33_07015"
     CDS             2262..2840
                     /locus_tag="VNQ33_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWJ97894.1"
                     /translation="MGLDFSNGLESLDHNFVTAKVEDVVKWARTRSMWPATFGLACCA
                     IEMMGTGGAHYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQIYDQMMEPKWV
                     ISMGVCASSGGMFNNYAIVQGVDQVVPVDVYAPGCPPGPETLIHAIGTLHAKIEDGDI
                     FRRRAENGGGAAIEVTQLDGQRGPQPILLGRS"
     gene            2843..3373
                     /locus_tag="VNQ33_07020"
     CDS             2843..3373
                     /locus_tag="VNQ33_07020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWJ97895.1"
                     /translation="MAETTTATDEPVAPGAAPEPELLHGAPVVHVRGQRVVHATRDEL
                     VGLVRTLRGEGWLQCLDVTAVDYLANAHRELPEGIEAERFEVVVLLISHSARERLRIR
                     VQVPESDPVVPTLFEVHPGTEASEREVFDMFGIRFDGHPDLTRILMPEDWEGHPLRKD
                     YGIGRIPVQFKDAPDA"
     gene            3370..4686
                     /locus_tag="VNQ33_07025"
     CDS             3370..4686
                     /locus_tag="VNQ33_07025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWJ97896.1"
                     /translation="MTATEESSHLTDEGAQEMAPRIVAGGTEVLREVGSVLRISEAEA
                     GEDAPDDAETTIINMGPQHPSTHGVLRLMLELEGETVLRTKPVIGYLHTGMEKTGESL
                     TYLQGGTNVTRMDYASPIFNELAFCLSAEQLLEIELPPRAVWIRMLMSELNRLTSHFL
                     FLATNGMDLGSTSMMIYGWREREEGLRILEMITGLRMNHNFIRIGGTAADLPDGWQQR
                     LTDYLDSIPERLEEYEILMTNQPIWRERLQGVGSITAEEALALGATGPILRSTGYAWD
                     LRREMPYLAYDQVDFDVIVGTYGDCFDRYAIRLNEVRESVRILRQVIEMMPAGDYRIQ
                     DKKVTPPPRARIDESMEALIHHFKIFTEGFKVPEGDAYAAVESPRGELGVYLVSDGSA
                     KPYRMHVRGPSFVNLQVLPHLMHGGLIADAVAIISSVDPIMGDVDR"
     gene            4683..>4839
                     /locus_tag="VNQ33_07030"
     CDS             4683..>4839
                     /locus_tag="VNQ33_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWJ97897.1"
                     /translation="MSRFSGPALATAHEILARYPVRKSATIPLCHLAQEQDGWLTEDA
                     MAHIAELV"
BASE COUNT          781 a         1775 c         1560 g          723 t
ORIGIN      
        1 gagggcgtcg gcggtgccac gccctacacc ctcaccgact cgcagggccg cttcatcaag
       61 agcgtcacct ggcaggggcc ggcgctcaac acggtcttca tgcgctacag ccgtgacgag
      121 gtgcgctaca tcctcaccta cggccgtccg ttctcgccga tgccggcgtg gggtaccgcc
      181 ggtggcggcc cgctcaccga gcagaagctc caggagctga tggactacat ctccaccttc
      241 cagctgacgc ccgcggaatc gcagaaggcg gtcaccgcgc agctcgaggt gatgatgaag
      301 aagaaggacc agacctgcgt cgacgccaag accgacgagg ccaagtccgc cctccccgag
      361 ggcgagacct tcgacgagac caaggtcgac acctccgact gcccgcccat gtacaagtcc
      421 gagggcgacg ccctgttcaa catgggctac gacgacggct tcgccggcgg cgcctactcc
      481 tgcggccgct gccacacgaa gggctggtcc tacggcgaga aggccgccga cggctcgggt
      541 gccttcggtc cgccgctcag caacgtcctc cagcagttcc ccggcgccac gctcggcccg
      601 aaggatcaga cggacttcgt ctgcaccggc tcggaccagg gcgtgcgcta cggccagacc
      661 ggccagggca cgggccgcat gcccgggttc tgcatcaccc cgggttacaa gatcaacccg
      721 ctgaacggcg aggtcggcat cgagccgttc gacaccggga cagcggagca aggcgccatg
      781 ttcacccagg accaggtgcg caagatcgtg gagtacgaac ggagcctcgc acgtgactag
      841 tctgctcgcc gccctcgcgt gggacccggg cttccggggc atcttgacgg tcgccgtcgc
      901 ggtggccatc ttgtgcgggt cggtgttcct catcctcgcc accaacaacg gcgcccgcct
      961 cgggttcctg ctggcgctca ccggcttctg cggctggatg ttcgtcctcg ggaccatctg
     1021 gtcgatgtac ggcatcggct ggaagggctc ggcgcccacc tggaaggtgg tcgacgtcgt
     1081 ccgcagcgag cccggcagcg gggagatcgt gtcctccttg gaggcggcag agtccctgcc
     1141 gctccccgac gagctccccg acgccgtcga gctgcgcgac agcagcaagg tgttcgccga
     1201 gaacttcccg gccacccagc gtgatccgaa ggtcggtgac ctcgtgtcga tcgacgatcc
     1261 ggaggccaag gagctcaagg agaagatcaa cgcccaggtc gccccgtggc ggatcctcga
     1321 gacgtcgaac aagtacaccg gtgagaccca ggcgttcgtg tccacggccc tcggccccga
     1381 cgggcaggcc atcttcgagt cggccagcga ctacgtcgtg ttggactcgt tcctcgtcgg
     1441 cggcaagaag gcccggaccg acaacagcac catcggccgg gtcaggtgga agctcggctc
     1501 gatcgtgaac cagagcccac cggacctcta cgccgccgtc cagctgcagg cggtcatccc
     1561 gcaggagacg aaggacggcc aggcgccgcc cacgccggtc gcgaacccca aggccgacat
     1621 catcaccgtg gtgctcaagc gcatcgacgg ccaccacctg cgccgcaacg ccatcggcat
     1681 gacgatcttc atgggcgccg ccacggccgt cttgtgctcg atgctgcacc gccgcgacaa
     1741 gctcgctcag gtgcagcggg cccagacggc gggagccgcc tgaccatggg ccagtacctg
     1801 cccatcctcg tgatgctcgt cttgggcgtg ctcttcgccg gcgtcagcct ggtgatgtcg
     1861 cagctggtca gccccaagcg gccgtcggcg gcgaagtcgg cgccctacga gtgcggcatc
     1921 gtgccgcggc aggaaccccc ggagcgcttc ccggtgcgct tctacctggt ggcgatgatc
     1981 ttcgtggtcg tcgacatcga gatcatcttc ttgtacccgt acgtggtcat ccaccgcgag
     2041 ctgggcgtct tcggcctcat cgagatgctc gtcttcgcca tcgcggtgtt cgcgtcgttc
     2101 gtgtacctga tcgcccacgg cgccctcgac tgggggccca tcgcccagct gcggcgctcg
     2161 tcgaaccctg ccgtcagcac cgagcgcacc gcggcgtcca ccgtgcgccg ggtcggccaa
     2221 gaggggcgcg ggcccaaggc gcccagcgag gtggcggcct gatggggctc gacttcagca
     2281 acggcctcga gtcgctcgac cacaacttcg tcaccgccaa ggtggaagac gtcgtcaagt
     2341 gggcgcggac ccgcagcatg tggccggcca cgttcggcct ggcctgctgc gccatcgaga
     2401 tgatgggcac cggcggcgcc cactacgacc tcgcccgctt cggcatggag gtcttccgcg
     2461 cctcgccgcg ccaggcggat ctgatgatcg tcgccggccg cgtgtcgcag aagatggccc
     2521 ccgtcctgcg ccagatctac gaccagatga tggagcccaa gtgggtcatc tccatgggcg
     2581 tctgcgccag ctcgggcggc atgttcaaca actacgcgat cgtccagggc gtcgaccagg
     2641 tcgttcccgt cgacgtgtac gccccgggct gcccgcccgg gcctgagacg ctcatccacg
     2701 ccatcggcac cctccacgcc aagatcgaag acggcgacat cttccgccgc cgcgccgaga
     2761 acggcggcgg tgcggccatc gaggtgacgc agctcgatgg ccagcgcggc ccccagccca
     2821 tcctcctggg tcggtcctga ccatggccga gaccaccacc gccaccgacg agccggtggc
     2881 gcctggcgcg gcgcccgaac cggagctgct ccacggcgcc cccgtcgtcc acgtccgggg
     2941 ccagcgggtc gtccacgcca cccgcgacga gctggtcggc ctggtccgca ccctgcgcgg
     3001 cgagggctgg ctccagtgcc tcgacgtcac cgcggtcgac tacctcgcca acgcccatcg
     3061 tgagctgccc gagggcatcg aggccgagcg cttcgaggtc gtcgtgctgc tcatctcgca
     3121 cagcgcccgc gagcggctcc gcatccgcgt gcaggtcccc gagagcgatc ccgtcgtgcc
     3181 gaccctcttc gaggtgcacc ccggcaccga ggcctccgag cgcgaggtgt tcgacatgtt
     3241 cggcatccgc ttcgacggcc acccggacct gacccggatc ctcatgcccg aggactggga
     3301 agggcacccg ctccgcaagg actacggcat cgggcggatc ccggtgcagt tcaaggacgc
     3361 tcccgacgca tgaccgccac cgaagagtcc tcccacctca ccgacgaagg cgcccaggag
     3421 atggcgcctc gcatcgtcgc gggcggcacc gaggtgctcc gcgaggtcgg ctcggtgctg
     3481 cgcatctccg aggccgaggc gggcgaggac gctcctgacg acgccgagac caccatcatc
     3541 aacatgggtc cccagcaccc gagcacgcac ggtgtgctcc gcctgatgct ggagctcgag
     3601 ggcgagaccg tcctgcgcac caagcccgtc atcggctacc tgcacaccgg catggagaag
     3661 acgggcgagt cgctcaccta cctccagggc ggcaccaacg tcacgcgcat ggactacgcg
     3721 tcgccgatct tcaacgagct ggcgttctgc ctgtccgctg agcagctgct cgagatcgag
     3781 ctcccgccgc gagcggtctg gatccggatg ctgatgagcg agctgaaccg gctcacctcg
     3841 cacttcctct tcctcgccac caacggcatg gacctcgggt ccacgtcgat gatgatctac
     3901 ggctggcgcg agcgcgaaga gggcctgcgg atcctcgaga tgatcaccgg gctgcggatg
     3961 aaccacaact tcatccgcat cggcggcacc gcggccgacc tgcccgacgg gtggcagcag
     4021 cgcctcaccg actacctcga ctccatcccc gagcggctgg aggagtacga gatcttgatg
     4081 accaaccagc ccatctggcg cgagcgcctc cagggcgtgg ggtccatcac cgccgaggag
     4141 gccctggccc tcggcgccac cggcccgatc ctgcggtcca cgggctacgc ctgggacctg
     4201 cggcgcgaga tgccctacct ggcgtacgac caggtcgact tcgacgtcat cgtggggacc
     4261 tacggcgact gcttcgaccg ctacgccatc cgcctcaacg aggtgcgcga gtcggtgagg
     4321 atcctccgcc aggtcatcga gatgatgccg gcgggcgact accgcatcca ggacaagaag
     4381 gtcacgccgc cgccgcgcgc tcgcatcgac gagtcgatgg aggcgctgat ccaccacttc
     4441 aagatcttca ccgagggctt caaggtcccc gagggcgacg cctacgcggc ggtcgagtcc
     4501 cctcgcggcg agctgggcgt gtacctcgtc tccgacgggt cggccaagcc ctaccgcatg
     4561 cacgtccggg ggccgagctt cgtgaacctg caggtcctgc cgcacctcat gcacggcggc
     4621 ctcatcgccg acgccgtggc catcatctcg tccgtcgacc ccatcatggg ggacgtcgac
     4681 cgatgagccg cttctccggg ccggcgctcg ccacggcgca cgagatcctg gcccgctacc
     4741 ccgtgcgcaa gtcggccacc atccccctgt gccacctggc ccaggagcag gacggctggc
     4801 tcaccgagga cgccatggcc cacatcgccg agctggtgg
//
LOCUS       DATMFS010000380         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_292607, whole genome shotgun sequence.
ACCESSION   DATMFS010000380 DATMFS010000000
VERSION     DATMFS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292607"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VNQ33_07035"
     CDS             complement(<1..639)
                     /locus_tag="VNQ33_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ97898.1"
                     /translation="MATQPVAERPDSFNDLHRALARTIGEVIQGKPEAIDLVLLALIA
                     EGHILVEDVPGVGKTTLAKALARSIDGEYGRIQFTPDLLPSDVVGVNVWNRATGEFDF
                     RRGPVFANVVLADEINRASPKTQSALLECMEERQVSIDGHTHILDAPFSVIATQNPIE
                     MEGTYALPEAQRDRFMARVSMGYPDARAEAAMLSARELVSPLDTLESVVTRDE"
     gene            750..1004
                     /locus_tag="VNQ33_07040"
     CDS             750..1004
                     /locus_tag="VNQ33_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97899.1"
                     /translation="MTSSGWWVQAALAVLRRPSLWGVAVTQALRLAQPGWWRRSPFLP
                     VPDPAYLRFRLETQYGSDHEPDPSDVVTYLHWCRTFRHVA"
     gene            1001..>2046
                     /locus_tag="VNQ33_07045"
     CDS             1001..>2046
                     /locus_tag="VNQ33_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWJ97900.1"
                     /translation="MSAPGTGTPSAPVLTAVELVRVRLPLRHAHRAAHGSEAVRDLVL
                     VRAGFDDGTSGWGECSALARPTYTSEHTAGAWLVLRDEIVPAVFAGRAPQVVGHPMAS
                     AAVLTALSDAVLQRVERSLATDLAEAHGTNPVRAVQTCAVISRSDGLDDVLARVDERV
                     AARVAMVKLKVTPDPQDLAAVGAVREAWPDLALAVDFNGTADADALHQLAPLHLEYVE
                     QPAPADELVRSAHLASLIEAPVALDESLTSPGALDTAVALGAGRIANIKPARCGGPHA
                     GAALVAQARNAGLEVFVGGMVESGVGRAAALAVAALPGCTLPTDLGPSLAYFDEDVTE
                     PLLTDGSGRMIVPMA"
BASE COUNT          240 a          767 c          740 g          299 t
ORIGIN      
        1 ctcgtcgcgg gtgaccaccg actcgagcgt gtcgagcggg ctgacgagct cgcgtgcgct
       61 cagcatcgcg gcttcggcgc gagcatccgg gtatcccatc gacacgcgcg ccatgaagcg
      121 gtcgcgctgc gcctcgggca gcgcgtaggt gccctccatc tcgatcgggt tctgcgtcgc
      181 gatcacgctg aagggggcgt cgaggatgtg cgtgtgcccg tcgatcgaca cctgacgctc
      241 ctccatgcac tccagcagcg ccgactgcgt cttcggcgac gcccggttga tctcgtcggc
      301 gaggacgacg ttggcgaaca cggggccgcg gcggaagtcg aactcgccgg tggcccggtt
      361 ccacacgttg acgcccacca cgtcggacgg gagcaggtcc ggggtgaact ggatccgccc
      421 gtactcgccg tcgatcgacc gggccagcgc cttggccagg gtggtcttgc cgacgcccgg
      481 gacgtcctcc acgaggatgt ggccctcggc gatgagggcg agcagcacga ggtcgatcgc
      541 ctcgggcttg ccctggatca cctccccgat cgtgcgggcg agggctcggt ggaggtcgtt
      601 gaagctgtct ggtcgctccg ccacgggttg cgttgccacg cgctgctcct gatcgaagat
      661 gagccgccca cgatagaggc ccgcggcgcg gacgtccgcc gcggtgtccg ggagccggcg
      721 tgccaagctg cgttcgtgga gcgagcgtcg tgacatcgag cgggtggtgg gtccaggcgg
      781 cgttggccgt cctgcgccga ccgtcgctgt ggggcgtggc ggtcacccag gccctgcgcc
      841 tggcccagcc ggggtggtgg cggcggtcgc cgttcctccc ggtgccggac ccggcctacc
      901 tccgcttccg gctggagacc cagtacggct cggaccacga gcccgaccca tcggacgtcg
      961 tcacctactt gcactggtgc cggaccttcc gccacgtcgc atgagcgcac ccggcaccgg
     1021 cacgccgagc gcacccgtcc tcacggcggt cgagctggtg cgggtgcggc tgccgctgcg
     1081 ccacgcccac cgtgccgcgc acgggtccga ggccgtgcgg gacctggtgc tggtccgggc
     1141 cggcttcgac gacggcacgt ccgggtgggg cgagtgctcg gccctggccc ggcccaccta
     1201 cacgtccgag cacaccgccg gcgcctggct ggtcctccgc gacgagatcg tgccggccgt
     1261 gttcgccgga cgggccccgc aggtggtcgg gcacccgatg gcgtcggccg cggtgctcac
     1321 cgccttgagc gacgccgtcc tccagcgggt cgagcgctcg ctcgccaccg acctggccga
     1381 ggcgcacggg acgaacccgg tccgggccgt ccagacgtgt gcggtcatca gccggtccga
     1441 cggcctcgac gacgtcctcg cccgggtcga cgagcgcgtc gcggcgcggg tggcgatggt
     1501 gaagctcaag gtcacgcccg acccgcagga cctggccgcg gtgggcgccg tgcgggaggc
     1561 gtggcccgac ctcgccctcg ccgtcgactt caacggcacg gccgatgccg acgccttgca
     1621 ccagctggcg ccgctccacc tcgagtacgt ggagcagccg gcgccggccg acgagctggt
     1681 tcgctccgcc cacctcgcct cgctcatcga ggctccggtc gcgctcgacg agtcgctgac
     1741 gtcgcccggc gcgctggaca cggccgtggc gctcggcgcc gggcgcatcg ccaacatcaa
     1801 gcccgcccgc tgcggcggtc cccacgccgg cgccgccctc gtggcccagg cccgaaacgc
     1861 cgggctcgag gtgttcgtcg gcgggatggt cgagtcgggc gtcggccgtg ccgccgccct
     1921 cgccgtcgcc gcccttcccg gctgcacgct gcccacggac ctgggcccgt cgctggccta
     1981 cttcgacgag gacgtgaccg agccgctcct caccgacggc tccgggcgca tgatcgtccc
     2041 catggc
//
LOCUS       DATMFS010000381         3682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_152840, whole genome shotgun sequence.
ACCESSION   DATMFS010000381 DATMFS010000000
VERSION     DATMFS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152840"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VNQ33_07050"
     CDS             <1..739
                     /locus_tag="VNQ33_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97901.1"
                     /translation="RIVLLSSATVYGPWANNPVPLTEDAPLRPHPDLAYAVQLAEVER
                     VAAEWSGDHPAAAVARLRPVPVVEDGRLGWLARAFDAAADVPTADDTPGQYLHLADLA
                     AAVVTAWDARLDGPVNVAPAGWLTPTQRRALDPVPRLRLPEPVALKIASWRWRLGWAP
                     TPPGVLPYVRHPWVVANDRLVAAGWSETSSNEEAYVVGHEASAIESISPRRRQELALG
                     VAGAVGAGAVVVAVALVRRRRRRRRTT"
     gene            complement(866..2059)
                     /locus_tag="VNQ33_07055"
     CDS             complement(866..2059)
                     /locus_tag="VNQ33_07055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWJ97902.1"
                     /translation="MAFDDPGEEERPDTTPPLPPDDRLWRHPSELAGGVPAPAAWSPP
                     SAPQPHRGSRVVVAMASAGLAGALVAAGVMWFARPTRVVVEQGEPTAAKTTATAVFAS
                     AGVPSEALATRLAPSLVHVEAEQGSTWSTGTGIRLDDDGTIAVATPVVAGATTVMVTD
                     HDGDRVRAKTIGADAATGITVLIVTGEGGTPVAARSTDAKAGQAVAVIGAASVSSDGT
                     TQQRVVTASVSAIGQRTTVKPIVLHDAVQLDRAVPDDATGGIVVDADGRLVGIVLDGS
                     GAEDLAVVVPADDAMAAARGLRDDGAVRRAWLGVRAVDLSPAAAELMSVKGGAQLTMV
                     QDGSPAAAAGLHQGDVITSVDGDPIGDASDLVVALRQWKPGEQVDVEWRRGAEDGHAA
                     ITLGG"
     gene            2125..2643
                     /locus_tag="VNQ33_07060"
     CDS             2125..2643
                     /locus_tag="VNQ33_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWJ97903.1"
                     /translation="MTVDAAATWPLPRGADWLACAPGRLPVEQASSWVVTPATGASVT
                     FVGTARDHAGDRTGVHQLEYEAYDEQVVPVLARVAAATRTRFPDVVRVALWHRTGTLE
                     ISEAAVVVAVSSAHRDAAFEAARWAIDEVKATAPIWKREHWTGGHDDWGRCDHRAGHD
                     VDGLHQTPAVAS"
     gene            2640..2897
                     /locus_tag="VNQ33_07065"
     CDS             2640..2897
                     /locus_tag="VNQ33_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97904.1"
                     /translation="MNILLFVLVPILAVSAYAGFVWARNREPSSVESGVDAFRREMTA
                     LSPDAAPVQRRPDRGGPSGGAPRQAPRRPSSGDGNPPAGGR"
     gene            2897..>3682
                     /locus_tag="VNQ33_07070"
     CDS             2897..>3682
                     /locus_tag="VNQ33_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWJ97905.1"
                     /translation="MARDLAIDLGTANTLVYAKGQGIVLNEPSVIALNQQTGDVLAMG
                     QEAWQMIGRTPGYIVAVRPLRQGAITDFDVTQRMIRLLLQRAGVSKFNRPRVVICVPS
                     AITEVERRAVTEAARRAGAADAQLIEQPMAAALGAYLPVNEPAANMVVDVGGGTSETA
                     LISLGGVVALQAVRVGSFDIDAAIQTFIRREYGIAIGERTAEEIKVAIGSAWPVQDDV
                     KAEVRGRDLMSGLPKTVILSPQEVREAISEPVSSIVDSVIACLG"
BASE COUNT          432 a         1390 c         1365 g          495 t
ORIGIN      
        1 acgcatcgtg ctgctgtcga gcgccaccgt ctacgggccg tgggccaaca acccggtccc
       61 gctcaccgag gacgccccgc tgcgccccca ccccgatctg gcgtacgccg tgcagctggc
      121 cgaggtcgag cgggtggcgg cggagtggtc cggagaccac cccgcggccg ccgtcgcccg
      181 gctgcggccg gtccccgtcg tcgaagacgg ccggctcggg tggttggccc gcgccttcga
      241 cgctgccgcc gatgtgccga ccgccgacga caccccgggc cagtacctcc acctcgccga
      301 tctggcggcg gcggtcgtca ccgcgtggga cgcccggctc gacggcccgg tgaacgtggc
      361 cccggccggc tggctcacac ccacccagcg gcgggcgctc gaccccgtgc cgcgcctgcg
      421 gctgccggag ccggtcgccc tgaagatcgc ctcgtggcgc tggcggctcg gctgggctcc
      481 gaccccgccc ggcgtgctcc cgtacgtccg gcacccgtgg gtggtggcca acgaccgcct
      541 cgtcgccgcc ggttggtccg agacctcgag caacgaagag gcctacgtgg tgggccacga
      601 ggcgagcgcc atcgagtcca tctcgccgcg ccgccgccag gagctggccc tcggcgtcgc
      661 cggtgccgtc ggcgcgggtg ccgtcgtggt ggccgtcgcc ctcgtgcgcc gccgccgccg
      721 tcgtcgccgc accacctgac ggtttcgggt cacgcgtgca ccgctgcggt gcccaggcga
      781 cccgattgcg aacggtggcc ggcgcgagcg cgctttcggg tcacgcgtgc accgctgcgg
      841 tgctcaggtg acccgaaaac gagggtcagc cgccgagggt gatggcggcg tggccgtcct
      901 cggcgccgcg ccgccactcg acgtcgacct gctcgccggg cttccactgc cggagcgcga
      961 ccaccaggtc gctggcgtcg ccgatcgggt cgccgtcgac gctcgtgatg acgtcgccct
     1021 ggtgcaggcc ggccgctgcg gccggtgagc cgtcttgcac catcgtcagc tgggcgccgc
     1081 ccttcaccga catgagctcg gcggcggccg ggctgaggtc gacggcccgg accccgagcc
     1141 aggcgcggcg gaccgcgccg tcgtcgcgga ggccgcgggc ggcagccatg gcgtcgtcgg
     1201 ccggcacgac gaccgccagg tcctcggcgc cggatccgtc gaggacgatg cccaccaggc
     1261 ggccgtcggc gtcgacgacg atgccgccgg tggcgtcgtc gggcaccgcc cgatcgagct
     1321 gcacggcgtc gtgcaggacg atcggcttga cggtggtgcg ctggccgatg gcgctgaccg
     1381 acgcggtgac cacccgctgc tgcgtggtgc cgtcggacga gacggacgcg gcgccgatga
     1441 cggccacggc ctgaccggct ttggcgtcgg tcgaccgggc ggcgacgggc gtgccgccct
     1501 cgcccgtcac gatgaggacc gtgatccccg tggcggcgtc ggcgccgatg gtcttggccc
     1561 ggacgcggtc gccgtcgtgg tcggtcacca tcacggtcgt ggcgccggcg acgacggggg
     1621 tggcgacggc gatggtcccg tcgtcgtcga ggcggatgcc cgtgcccgtg gaccaggtgc
     1681 tgccctgctc ggcctcgacg tggacgagcg atggcgccag gcgggtggcc agtgcctcgg
     1741 acggcacgcc ggccgaggcg aacacggcgg tggcggtcgt cttggccgcg gtgggctcgc
     1801 cctgttcgac cacgacgcgc gtcggccggg cgaaccacat gacgccagcg gccacgagcg
     1861 ccccggcgag cccggcgctg gccatggcga cgacgacacg tgagccccgg tggggctgcg
     1921 gagcagatgg cggagaccag gcggccggcg cggggacgcc gccggccagc tccgaggggt
     1981 ggcgccacag gcggtcgtcg ggaggcagcg gcggggtcgt gtcggggcgt tcctcctccc
     2041 ctgggtcatc gaacgccatg gctcgccacg ctaccgatcg gcgcgtgcgg gtgggccgcg
     2101 ccccgccccg cctacgatga cgcgatgact gtggacgccg cggccacctg gccgctgcct
     2161 cgtggcgccg actggcttgc gtgcgcgcct ggtcggctgc cggtcgagca ggcgtcgtcg
     2221 tgggtcgtga cgccggcgac cggtgcctcg gtcacgttcg tgggcaccgc tcgcgatcac
     2281 gctggcgacc gcacgggcgt ccaccagctg gagtacgagg cctacgacga gcaggtcgtc
     2341 ccggtgctgg cccgtgtcgc cgcggccacc cggacgcggt tccccgacgt ggtgcgcgtc
     2401 gccctctggc accgcaccgg caccctcgag atctccgagg ccgccgtggt cgtcgccgtc
     2461 tcgtcggccc accgcgatgc cgccttcgag gccgcccgtt gggccatcga cgaggtcaag
     2521 gccaccgccc ccatctggaa gcgcgagcac tggacgggtg gccacgacga ctggggccgc
     2581 tgcgaccacc gggccggcca cgacgtcgac ggcctccacc agaccccggc ggtggcgtcg
     2641 tgaacatcct cctgttcgtg ctggtcccga tcctcgccgt cagcgcctac gccgggttcg
     2701 tgtgggcccg caaccgcgag ccttcctccg tcgaatccgg cgtcgatgcg ttccggcgcg
     2761 agatgaccgc gctgtcgccc gacgccgcgc cggtccagcg ccgccccgat cggggcggcc
     2821 cctcgggtgg cgcaccccgt caggcccccc gccgaccgtc atccggtgac ggcaacccgc
     2881 cggcgggcgg tcgctgatgg cccgcgacct cgccatcgac ctcggcacgg cgaacaccct
     2941 cgtctacgcc aagggccagg gcatcgtgct caacgagccg tcggtgatcg cgctcaacca
     3001 gcagaccggc gacgtcttgg ccatggggca ggaggcgtgg cagatgatcg gccgcacacc
     3061 cggctacatc gtcgcggtgc ggccgctgcg ccagggtgcc atcaccgact tcgacgtgac
     3121 gcagcggatg atccgcctgc tgctgcagcg ggccggggtg tcgaagttca accggccccg
     3181 ggtcgtcatc tgcgtgccgt cggccatcac cgaggtggag cgccgggccg tcaccgaggc
     3241 agcgcgccgg gcgggggcgg ccgacgccca gctcatcgag cagcccatgg ccgccgccct
     3301 cggcgcctac ctgccggtca acgagccggc cgccaacatg gtcgtcgacg tcggcggcgg
     3361 tacgtccgag accgcgctca tcagcctcgg tggcgtcgtc gccctgcagg ccgtgcgcgt
     3421 cgggtcgttc gacatcgacg ccgccatcca gaccttcatc cgccgcgagt acggcatcgc
     3481 catcggcgag cggaccgccg aggagatcaa ggtcgccatc gggtcggcgt ggccggtgca
     3541 agacgacgtg aaggccgagg tgcgcgggcg cgacctcatg agcggcctgc ccaagaccgt
     3601 gatcctgtcg ccgcaggagg tccgcgaggc catctcggag ccggtctcct ccatcgtcga
     3661 ctcggtgatc gcctgtctcg gc
//
LOCUS       DATMFS010000382         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_108276, whole genome shotgun sequence.
ACCESSION   DATMFS010000382 DATMFS010000000
VERSION     DATMFS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108276"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNQ33_07075"
     CDS             <1..271
                     /locus_tag="VNQ33_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97906.1"
                     /translation="WTAPAKPGGRPNLGITHGSGPQALARLERFGVELEPGWLKVADH
                     PRRGLVVAGPSGVAPDHALWWLLTAAHVLSVVPLTGNWLARVYTP"
     gene            complement(292..1467)
                     /locus_tag="VNQ33_07080"
     CDS             complement(292..1467)
                     /locus_tag="VNQ33_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97907.1"
                     /translation="MSTPTVDPAPVLAFGDVRRAGRRVLARLDQLLAGRWWLVIVVLL
                     AAGVSLFCAFPTYSGYFPHGRDWLAIQHIADHPFDQTGLIPSDRPYNFTFRVLVPLVG
                     GRLGLTPPGYLALQACGALALFAGTARIVHRITESRRLAALSAIAVGLTWAGATGFVE
                     IRGNFDSIAIALLVWAMATRKVPLVVLFGFLATWTDERALPALAFVALYHHIVESRDI
                     RWYTALREPRVLAALGAAVLHVISRLAATAVFDLHQPSDLGFMNIKAQMSNLPVGAWT
                     GLEGMWLFVALGVVALWRVRERLLSLVYLGYVGIFALGAIAVVDVTRSASYLLPAGLA
                     AMAIVARLCDRRLLHHTTYAAFALSAAWPLYYAGGAYTMYWNYPLPLVIVRKLAGVE"
     gene            complement(1527..>2592)
                     /locus_tag="VNQ33_07085"
     CDS             complement(1527..>2592)
                     /locus_tag="VNQ33_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ97908.1"
                     /translation="LSVLDAAEQADLSVTGFLDLLERMHAADAAADASEAFGEPPEIS
                     VVVPMYNEEGNLVPLLAELVPVLESVGTYEVVFIDDCSTDRSRAVVLEQRALNPNVKL
                     VELARNFGHQGALSAGFDHATGNAVVLMDADLQDPPSVLPEMIERWRAGYEVVYAVRE
                     KRKEGPVLRASFFLFYRILQRISEIDLPLDSGDFCLMDRKVVDAIRNLPESNRFLRGL
                     RGWVGFRQVGVTYDRPARLTGETKYSIRSRVRLAIDGLLSFSDVPLRLASFAGFLTTM
                     AAVAYLLIAVGTYLVGGEVVKGWTSIIFVQLILGGVQLMILGVAGEYVSRIYQESKRR
                     PPYVLRTTHGARHVDRARSR"
BASE COUNT          431 a          869 c          977 g          315 t
ORIGIN      
        1 gtggacggca ccggccaagc ccggtggccg cccgaacctc gggatcaccc acggatccgg
       61 cccccaggcg ctggccaggc tggagcgctt cggggtcgag ctcgagccgg gctggctgaa
      121 ggtcgccgac cacccccggc gcgggctcgt ggtcgccggc ccgagcggcg tggcgcccga
      181 tcacgccctc tggtggctcc tcaccgccgc tcacgtgctc agcgtcgtcc cgctcaccgg
      241 caactggctg gcgcgggtct acacgccctg agctgagcgt cgctcagcgg ctcactcgac
      301 cccggccagc ttccgcacga tgaccagcgg aagcgggtag ttccagtaca tcgtgtacgc
      361 cccgcccgcg tagtagagcg gccaggcggc gctcagcgcg aacgccgcgt aggtggtgtg
      421 gtgcagcagt cggcggtcgc acaggcgggc cacgatcgcc atggcggcga gcccggcggg
      481 cagcaggtac gacgcgctgc gggtcacgtc gaccacggcg atggcgccca gggcgaagat
      541 cccgacgtag cccaggtaga cgagcgacag caggcgctca cgaacccgcc acagcgccac
      601 cacccccagt gccacgaaca gccacatccc ttcgagaccg gtccacgcac cgacgggcag
      661 gttgctcatc tgggccttga tgttcatgaa gccgaggtcg ctgggctggt gcaggtcgaa
      721 gaccgccgtc gcggccagcc ggctgatcac gtgcagcacg gcagcgccca gggcggcgag
      781 cacgcggggc tcccggaggg cggtgtacca gcggatgtcg cggctctcga cgatgtggtg
      841 gtacagggcc acgaacgcca gggccgggag cgcccgctcg tcggtccacg tcgccaggaa
      901 cccgaacagc accacgagcg gcaccttgcg ggtggccatg gcccacacca acagcgcgat
      961 cgcgatcgag tcgaagttcc cgcggatctc gacgaacccg gtggccccgg cccaggtgag
     1021 accgacggcg atcgccgaca gggcggcgag gcgccggctc tcggtgatgc ggtggacgat
     1081 gcgggccgtg ccggcgaaca gggccagggc accgcaggcc tgcagggcga ggtacccggg
     1141 cggcgtgaga ccgaggcggc cgccgacgag cggcacgagg acgcggaagg tgaagttgta
     1201 cgggcgatcg gaagggatca ggccggtctg gtcgaacggg tggtcggcga tgtgctggat
     1261 ggccagccag tcgcgcccgt gcgggaagta ccccgagtag gtcgggaagg cgcagaacag
     1321 cgagacgccc gccgcgagca ggaccacgat cacgagccac caccgccccg cgaggagctg
     1381 gtcgagccgc gccaggaccc gccggcccgc tcggcggacg tcgccgaagg ccaggaccgg
     1441 ggcgggatcg acggtcgggg tgctcatgga caggacctct cggtgggcgg cgggttgcag
     1501 gcaggacggg cggcgacggg cccgggtcag cgggaccggg cccggtccac gtggcgagcg
     1561 ccgtgcgtcg tgcggaggac gtagggcggt cggcgcttgg actcctggta gatgcgcgag
     1621 acgtactcgc cggccacccc caggatcatc agctggacgc cgccgaggat cagctggacg
     1681 aagatgatcg aggtccagcc cttcacgacc tccccaccca ccaggtaggt cccgaccgcg
     1741 atcagcaggt aggcgacggc agccatggtg gtgaggaatc cggcaaagga cgccaggcgc
     1801 agcggcacgt cgctgaacga gagcaggccg tcgatggcga ggcggacccg gctgcggatc
     1861 gagtacttgg tctcgccggt gagccgggca gggcggtcgt aggtgacgcc gacctggcgg
     1921 aagccgaccc agccgcgcag cccgcgaaga aaccggttgg actcgggcag gttgcggatg
     1981 gcgtcgacga ccttgcggtc catgaggcag aagtcgccgc tgtcgagcgg caggtcgatc
     2041 tccgagatgc gctggaggat ccggtagaag aggaagaagc tggcccgcag gaccggaccc
     2101 tccttgcgct tctcccggac ggcgtagacg acctcgtagc cggcccgcca gcgctcgatc
     2161 atctcgggca gcaccgaggg cgggtcctgg aggtcggcat ccatgaggac gacggcgttg
     2221 ccggtggcgt ggtcgaaccc ggcggacagc gcgccctgat ggccgaagtt ccgggccagc
     2281 tcgacgagct tcacgttcgg gttgagcgcc cgctgctcca gcaccaccgc ccgggagcgg
     2341 tcggtgctgc agtcgtcgat gaagacgacc tcgtaggtgc cgacggactc gagcacgggg
     2401 acgagctcgg ccaagagggg gacgaggttg ccctcctcgt tgtacatcgg caccaccacc
     2461 gagatctccg gcggctcacc gaacgcctcg ctggcgtcag ccgcggcatc ggcggcgtgc
     2521 atgcgctcca gcaggtcgag gaagccggtg acggagaggt cggcctgctc ggcggcatcg
     2581 aggacgctca gg
//
LOCUS       DATMFS010000383         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_13538, whole genome shotgun sequence.
ACCESSION   DATMFS010000383 DATMFS010000000
VERSION     DATMFS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13538"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VNQ33_07090"
     CDS             <1..657
                     /locus_tag="VNQ33_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWJ97909.1"
                     /translation="LVIRFFQKQGLAVAVAASSGVMNSLASGIVELVLILIAIVFTDL
                     SFSSARMGGSTSDIETYVLVGIVVIGVAVTVLVLAPRLRATLRRVVGPQVEAAKANLR
                     GILSDPRKAARIFGGQLCSQIAYALVIDAALHAYGSSLPLGTIVIVNSLASLLGGMAP
                     IPGGLGVIEAGLIAGMTAAGVEQSTAVAATFTARMCTAYLPPIWGWFALGWLRRNDYV
                     "
     gene            1009..1605
                     /locus_tag="VNQ33_07095"
     CDS             1009..1605
                     /locus_tag="VNQ33_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97910.1"
                     /translation="MSRTTAPIAIAIAACALVLGLTACGSSSRSAGQKASTQAETDRS
                     DGPETTTTTEGGGAASTTTVVAKGARAFCEDLAGYVDDRSGSDVDTTDRDGFKRSIQE
                     SARKGSEVLSRAPSELSGAVAAILDAQDQLIAALEKADYDMTKIPPAVLKAMHTPELE
                     EASDEVDAYIADTCGIDLEPRQIEAPEVTATTVASPDT"
     gene            1726..>2259
                     /locus_tag="VNQ33_07100"
     CDS             1726..>2259
                     /locus_tag="VNQ33_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ97911.1"
                     /translation="MGEGKATDDDATAPRLEGTVGVGGGRRIGFAEFGTPQGQAVIWL
                     HGTPGARRQIPVEARRFALEHDLRLVGIDRPGIGSSTPHLYGTMRDFAADLQAVADEI
                     GLDDLIVVGLSGGGPYALATGHAFPDRVRAVGVLGGVAPTTGPDAIGGGLVALGKRLS
                     PVIAVGRVPIGIGLTGVI"
BASE COUNT          308 a          833 c          796 g          322 t
ORIGIN      
        1 ctggtgatcc gcttcttcca gaagcagggc ctcgccgtgg ccgtcgccgc cagctcgggc
       61 gtcatgaact cgctcgccag cggcatcgtc gagctggtcc tcatcctcat cgccatcgtg
      121 ttcaccgacc tgtcgttctc atcggcgcgg atgggcggca gcacgagcga catcgagacg
      181 tacgtgctcg tcggcattgt cgtcatcggc gtggcggtca ccgtcctcgt gctggccccg
      241 aggctccggg cgacgctccg ccgcgtcgtc ggcccccagg tggaggcggc caaggccaac
      301 ctccgcggca tcctcagcga cccccgcaag gcggctcgga tcttcggtgg gcagctgtgc
      361 tcgcagatcg cctacgccct cgtgatcgac gccgccctcc acgcctacgg ctcgtcgttg
      421 cccctcggca cgatcgtgat cgtcaactcg ctcgcctcgc tgctcggcgg catggcgccc
      481 atccccggcg gcctcggggt gatcgaggcc ggtctgatcg ccggcatgac cgccgcgggg
      541 gtcgagcagt ccacggccgt cgccgccacc ttcaccgccc gcatgtgcac cgcctacctg
      601 cccccgatct ggggctggtt cgccctcggt tggctccgcc gcaacgacta cgtctagctg
      661 cccgcttcgc cgacccggtc gccctcgctg gacctggcat ccacctcggt gacgacatcg
      721 ccaggggtcg ggcacggtcg tggcgggcgc tgggcgccgg gcgccgggcg gcaggtggga
      781 gcgtacgagc gagagggcgc ggccgttcgc tccgggcggg tcgacgtcgt gcagccaccg
      841 cctccatcgg gacctcgggg gcggattgca gcgcgtcgtc cgtcgcagca ggatgccggg
      901 tgccgagcgg gggagaacgc cccaccctgg gctggtgcgt gggcccggtt ggaggtgccc
      961 ggatcggcga gtcgaaacgg cagctcgctc tggacgagga ggaagaccat gtcccgtacc
     1021 accgcaccga tcgccatcgc catcgccgca tgcgccctcg tgctcgggct caccgcctgc
     1081 ggcagctcgt cgaggtccgc tggccagaag gcgtcgaccc aggccgagac cgaccgcagc
     1141 gatggacccg agaccacgac gaccaccgag ggcggcggtg cagcgagcac cacgacggtg
     1201 gtcgccaagg gggccagggc gttctgcgag gacttggccg gctacgtcga cgacaggtcg
     1261 ggctccgacg tggacaccac cgaccgagac ggcttcaagc ggtccatcca ggagtcggcc
     1321 aggaagggga gcgaggtcct ctcccgtgct ccgagcgagc tcagcggcgc cgtcgctgcg
     1381 atcctcgatg cgcaggacca gctgatcgcc gcgctggaga aggccgacta cgacatgacc
     1441 aagatcccgc ctgcggtgct caaggcgatg cacacaccgg agctcgagga ggcgagcgac
     1501 gaggtcgacg cctacatcgc cgacacgtgc ggcatcgacc tcgagccccg gcagatcgaa
     1561 gcacccgagg tcaccgccac caccgttgcc tctcccgata cctgatcgga acggtccggc
     1621 ccagcaggtg cggcaccgcc cgcacggtgg cccgacctgg cggtcggcct cggccccatc
     1681 cggcgggatc gcgatcgacg ggacggacgg tggcaggctc tgtccatggg tgaggggaag
     1741 gccactgacg acgacgccac cgcgccgcgg ctcgagggca ccgtcggcgt gggcggcgga
     1801 cggcgcatcg gcttcgccga gttcggcacg ccccagggcc aggccgtcat ctggctccac
     1861 ggcacgccgg gcgcccgccg ccagatcccg gtcgaggccc gccggttcgc cctcgagcac
     1921 gacctgcgcc tggtcggcat cgaccggccc ggcatcggct cgtcgacccc gcacctgtac
     1981 ggcacgatgc gcgacttcgc tgcggatctc caagccgtgg ccgacgagat cggcctcgac
     2041 gacctcatcg tggtcggctt gtccgggggc ggtccgtacg ccctcgccac ggggcacgcc
     2101 ttcccggacc gggtccgggc ggtcggtgtg ctcggcggcg tggcaccgac cacgggaccc
     2161 gatgcgatcg gcggcggcct cgtcgccctg ggcaagcgcc tgtcgccggt gatcgcggtt
     2221 ggccgggtgc ccatcggcat cggtctgacc ggggtgatc
//
LOCUS       DATMFS010000384         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_267092, whole genome shotgun sequence.
ACCESSION   DATMFS010000384 DATMFS010000000
VERSION     DATMFS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267092"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VNQ33_07105"
     CDS             <1..1263
                     /locus_tag="VNQ33_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWJ97912.1"
                     /translation="PDATAAALLEQTGIIRVDNLTQMLFTANVLDSQPLPAGSRVAVL
                     TNSWGPAWLAVDALKAAGLHPHEPVDLSNEAAPEDFEAALTGIYQRGDVDAVLVIYAP
                     AIRAHYRRVGASLRSAARAAGAVTTVACMLGEHRAGLLRDDEVSIPEFPFPEEAAVAM
                     GRVARYAAWRSQPVGSLPELTDEHQVEARTLVDRWLDGPRRWLDPIESEQLLRCAGLA
                     PAAQALVETAEEAIDAARRLGFPVALKASGLERLSKTEAGGVSLDVHGDDEVRHAYER
                     MEALLGPAMHPAIVQVMVPEGVECRVGIYRHHVLGDVLTLGPGGAAADRVVEEALQIL
                     PLTDADAERLIDGSVLAPLVDEYGPEARAALVDLLCRLAALADAIPEIVAVRLNPVLV
                     VDGSAAITDARIALAPHVADPRPHVRRL"
     gene            complement(1350..>2000)
                     /locus_tag="VNQ33_07110"
     CDS             complement(1350..>2000)
                     /locus_tag="VNQ33_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97913.1"
                     /translation="SGAVHADWCYLIARTDPGARPHAGLSELVVDMRTPGIEVRPIVD
                     ATGDGHFCEVRFDGVRVPAVNLVGAENGSFRQVMRQMEHERGGIDRLVSNQRLYRDAL
                     PLADRSDARVRQEIAAIETGYRLGRLLVVREVLGQAPKQFSAAAKTACTEHEQRVAWF
                     CASVLGARGQAGAADDDLSRRVARNLAYAPAYTIMGGTTQILRTIIAERVLGLPRA"
BASE COUNT          245 a          771 c          720 g          264 t
ORIGIN      
        1 ccggacgcca ccgcggcggc cctgctggag cagaccggca tcatccgggt cgacaacctc
       61 acccagatgc tgttcacggc gaacgtgctc gacagccagc cgctcccggc cgggagccgc
      121 gtcgccgtgc tcaccaactc gtggggtccg gcgtggctcg cggtcgacgc cttgaaggcc
      181 gcgggccttc atcctcacga gcccgtcgac ctctcgaacg aggcggctcc cgaggacttc
      241 gaggccgcgc tcacgggcat ctaccagcgc ggcgacgtcg acgccgtgct ggtcatctac
      301 gcgccggcga tccgcgccca ctaccggcgg gtgggcgcgt ccctgcggtc cgccgcccgc
      361 gccgccgggg ccgtcaccac cgtcgcctgc atgctgggcg agcaccgggc cggtctcctg
      421 cgtgacgacg aggtgtcgat cccggagttc cccttcccgg aggaggccgc ggtggcgatg
      481 ggccgcgtgg ctcgctacgc cgcgtggcgg tcgcagcccg tgggctcgct ccccgagctc
      541 accgacgagc accaggtcga ggcccggacc ctggtggacc gctggctcga cggcccgcgg
      601 cggtggctcg acccgatcga gtccgagcag ctgctccgct gcgcggggct ggctcccgcg
      661 gcccaggccc tggtggagac cgcggaggag gcgatcgatg ccgcccgccg cctgggcttc
      721 cccgtcgccc tcaaggcatc ggggctcgag cgcctgtcga agacggaggc cggcggcgtg
      781 tccctcgacg tccacggcga cgacgaggtg cgccacgcct acgagcggat ggaggccctg
      841 ctcggcccgg ccatgcaccc ggccatcgtg caggtgatgg tgccggaggg cgtcgagtgc
      901 cgcgtcggca tctaccgcca ccacgtgctc ggcgacgtgc tcaccctcgg ccccgggggt
      961 gcggccgccg atcgggtggt ggaggaggcg ctgcagatcc tgccgctcac cgacgccgac
     1021 gccgagcgcc tcatcgacgg gtcggtcctg gcgccgctcg tcgacgagta cggccctgaa
     1081 gcccgggccg cgctcgtcga cctcctgtgc cggctggccg ccctcgccga tgcgatcccc
     1141 gagatcgtgg ccgtgcgcct caacccggtc ctcgtggtcg acggctccgc ggccatcacc
     1201 gacgcccgca tcgccctcgc cccccacgtc gccgaccccc gcccccacgt ccgccgcctc
     1261 tgaaccaggg ggccgggagt cctgagcgcg gtcgatcccg cccccccccg aggtggaagg
     1321 ccgaccacag ccgggaaggc ggacgggact caggctcggg ggaggccgag gacgcgctcg
     1381 gcgatgatcg tgcggaggat ctgggtggtg ccgcccatga tcgtgtacgc cggtgcgtag
     1441 gcgaggttgc gcgccacccg gcggctcagg tcgtcatcgg cggcaccggc ctggccccgg
     1501 gcgcccagca ccgacgcgca gaaccacgcc acccgctgct cgtgctcggt gcacgcggtc
     1561 ttggccgccg ccgagaactg cttcggtgcc tggcccagca cctcgcgcac cacgagcagc
     1621 cggccgagcc ggtagccggt ctcgatcgcg gcgatctcct ggcggacccg ggcatcggag
     1681 cggtcggcca gggggagggc gtcgcggtag aggcgctggt tgctcaccag ccggtcgatg
     1741 ccgccgcgct cgtgctccat ctggcgcatc acctggcgga agctgccgtt ctctgcgccc
     1801 acgaggttca ccgccggcac ccgcacgccg tcgaaccgca cctcgcagaa gtggccgtca
     1861 ccggtggcgt cgacgatcgg gcggacctcg atccccggcg tccgcatgtc caccaccagc
     1921 tccgacaggc cggcgtgggg ccgggcgccc ggatcggtgc gggcgatgag gtagcaccag
     1981 tcggcgtgga cggcgccgct
//
LOCUS       DATMFS010000385         3284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_222631, whole genome shotgun sequence.
ACCESSION   DATMFS010000385 DATMFS010000000
VERSION     DATMFS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222631"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VNQ33_07115"
     CDS             complement(<1..844)
                     /locus_tag="VNQ33_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWJ97914.1"
                     /translation="MALEPNAVVARTRAAWDDDIQPTLEDYIRVPALSSAFDPGWAEH
                     GHLDAVVDSAAAWAARRAIAGLSVEVVRLPDRTPVLWFEVPAFDGAAASTDAASTDAG
                     ADADTVLLYGHLDKQPPMHGWRDGLGPWTPVVVEDRLYGRGGADDGYAAYASLTAIEA
                     VQADGGTHARCVGLIECSEESGSPDLPAYVSHLADRIGSPSLVVCLDSFCGDYERMWT
                     TTSLRGLLSATVEVRVLHEGVHSGSASGVVPDSFRIARRLLDRVEDPDTGRVLVDELW
                     ADVPP"
     gene            984..2459
                     /locus_tag="VNQ33_07120"
     CDS             984..2459
                     /locus_tag="VNQ33_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWJ97915.1"
                     /translation="MTASNRPRGARWGALLLGVLLVFSSCGVEAGKRDASSTRTTGSS
                     DIVGSDVEDTTTTSPTTTAPPPPTDVDIAGSDGSELNDVIGNAISDLEAFWTDAFPEA
                     YGDEYEPLSGGLFAIDSSTDPSNIPCASGPDITGLLDNAFYCPPADAVVWDQERFMPD
                     LAKRYGDFTVAVVIAHEWGHAIQERAAFSAASVTTELQADCFAGSWVKHVDDGDSQRF
                     TVTTDELDEALAGVLSLRDAPGGVADDPSAHGSGFDRVGAFQDGFENGVTRCAEFTDG
                     DPAPYEFPFSDQAELDTGGDLHLSGPTDDPGIIELAFPSLDAYWTDTFPTVSGGDDWD
                     PLGDPVGFSADDPPTCGGDPIKGYLLFVCIPDRFVGFESDKTIPAVYEQGGDFAVATL
                     FATQYGLDAVEQLGTADDEVRATLQGDCFAGAWAAALLPPDPPEDYQLVLSPGDLDEG
                     VAVLLSFRSEGDRERQGPGFNRVKAYRTGVLQGAKACTKLD"
     gene            2476..>3284
                     /locus_tag="VNQ33_07125"
     CDS             2476..>3284
                     /locus_tag="VNQ33_07125"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWJ97916.1"
                     /translation="MTEPISAATPPLPDAIDPAKVEALLTRVRREVDEGLSPAVQIAV
                     AHDGRVVVDETFGARADSRFLAFSATKALVAAAVWRLIDAGEVDIAATAASYVPEFAS
                     NGKDVVTVENLLTHTGGFPWAPLGPNRWSTTAGRREAFAKWRLTLDVGTAFMYHPTAG
                     HWVLGEILSSVTGMDHTDAIEELVTAPLGLPRLLGIALDQQDGIQEAVGIGVPPTPEE
                     MEEAYGVKVDIAALIPPDVALGALTTLNHPEARVIGVPGGGGIMRAADLALL"
BASE COUNT          467 a         1223 c         1156 g          438 t
ORIGIN      
        1 caggcgggac gtcggcccag agctcgtcga ccaggacccg gccggtgtcg gggtcctcga
       61 cacggtcgag caggcggcgg gcgatgcgga acgagtccgg caccacgccg ctggcgctgc
      121 cggagtggac gccctcgtgg aggacccgca cctcgacggt ggcgctcaac aggccgcgca
      181 gcgaggtggt ggtccacatg cgctcgtagt cgccgcagaa cgagtccagg cacaccacca
      241 ggctcgggct accgatgcgg tcggccaggt gggagacgta ggccgggagg tccgggctgc
      301 cggactcctc gctgcactcg atgagcccga cgcaccgggc gtgggtcccg ccgtcggctt
      361 gcacggcctc gatggcggtg agcgaggcgt aggcggcgta gccgtcgtcg gcgccgccgc
      421 ggccgtagag ccggtcttcg acgaccaccg gggtccacgg cccgaggccg tcacgccacc
      481 cgtgcatcgg cggctgcttg tcgaggtggc cgtagaggag gacggtgtcg gcatcggcac
      541 cggcatcggt cgacgcggca tcggtcgacg cggctgcgcc gtcgaacgcc gggacctcga
      601 accacagcac cggggtccgg tcggggaggc ggaccacctc caccgacagg ccggcgatgg
      661 cgcgccgagc ggcccacgcc gccgcgctgt cgacgacggc gtcgaggtgg ccgtgctcgg
      721 cccaaccagg gtcgaacgcc gacgacagcg ccggcacccg gatgtagtcc tcgagggttg
      781 gctggatgtc gtcgtcccag gcggcacggg tgcgggcgac gacggcgttc ggttcgaggg
      841 ccatggcccg agcatcgcag acacccgtcg cccgggggcg ctcgacgggt gggatcgcaa
      901 gcgtcggcgg gcttcggcca gctttcgggt ccgacgtgct ggaatggtgc gccgcagccc
      961 agggcgggct tggaggaggt ctcgtgacgg catcgaaccg gccgaggggg gcgcgctggg
     1021 gggcgttgct gctcggcgtc ctgctcgtct tcagctcgtg cggcgtcgag gccggcaaga
     1081 gggacgcgtc cagcacccgc acgacgggca gctccgacat cgtcggcagc gacgtcgagg
     1141 acaccacgac cacctcgccg accaccacgg cgccaccgcc gcccaccgac gtcgacatcg
     1201 ccgggtccga cggcagcgag ttgaacgacg tgatcggcaa cgccatcagc gatctcgagg
     1261 ccttctggac cgacgccttc cccgaggcct acggcgacga gtacgagccc ctgtcgggcg
     1321 ggctgttcgc catcgacagc agcacggacc cgagcaacat cccgtgcgcg tccggccccg
     1381 acatcaccgg gctgctcgac aacgccttct actgcccgcc cgccgacgcc gtcgtgtggg
     1441 accaggagcg gttcatgccg gacttggcca agcgctacgg cgacttcacc gtcgccgtgg
     1501 tcatcgccca cgagtggggc cacgccatcc aggagcgggc ggcgttctcc gccgcgtcgg
     1561 tgaccaccga gctccaggcc gactgcttcg ccggctcctg ggtcaagcac gtcgacgacg
     1621 gcgacagcca gcgcttcacc gtcaccaccg acgagctcga cgaggccctc gccggcgtgc
     1681 tctccctgcg cgacgctccc ggcggcgtgg ccgacgaccc gtctgcccac ggcagcgggt
     1741 tcgaccgcgt cggcgcgttc caggacgggt tcgagaacgg cgtcacccgg tgcgccgagt
     1801 tcacggacgg cgatccggcg ccctacgagt tcccgttctc ggatcaggct gagctcgaca
     1861 cgggcggcga cctccacctc tccgggccca cggacgaccc ggggatcatc gagctggcgt
     1921 tcccgtcgct cgatgcctac tggaccgaca ccttcccgac cgtcagcggc ggcgacgact
     1981 gggacccgct cggcgacccg gtcgggttct cggccgacga tccgcccacc tgcggcggcg
     2041 atccgatcaa gggctacctc ctgttcgtgt gcatccccga ccgcttcgtc ggcttcgagt
     2101 cagacaagac catcccagcc gtctacgagc agggcggcga cttcgccgtg gccaccctct
     2161 tcgccacgca gtacgggctc gacgcggtcg agcagctcgg cactgccgac gacgaggtgc
     2221 gcgccaccct ccagggcgac tgcttcgccg gtgcgtgggc ggcggcgctg ctgcccccgg
     2281 acccgccgga ggactaccag ctcgtcttgt cgcccggcga tctcgacgag ggcgtggccg
     2341 tgctgctgtc cttccgctcc gagggcgatc gcgagcgcca agggcccggc ttcaaccggg
     2401 tgaaggccta ccggaccggc gtgctccaag gcgccaaggc ctgcacgaag ctcgactagg
     2461 gcctaccgtc cgggcgtgac cgagcccatc tccgccgcca ccccgccgct gccggacgcg
     2521 atcgatccgg ccaaggtcga ggccctgctc acccgcgtcc gacgcgaggt ggacgagggc
     2581 ctgtcgcccg ccgtgcagat cgccgtcgcc cacgacggac gggtcgtcgt cgacgagacg
     2641 ttcggcgctc gggcggacag ccgcttcctc gccttctccg ccacgaaggc cctggtggcc
     2701 gccgccgtct ggcggctgat cgacgccggt gaggtggaca tcgccgcaac ggccgccagc
     2761 tacgtgcccg agttcgccag caacggcaag gacgtcgtca ccgtcgagaa cctgctgacc
     2821 cacaccggcg ggttcccctg ggcgccgctc ggcccgaacc gctggtccac caccgccggg
     2881 cgccgggagg ccttcgccaa gtggcggctc accctcgacg tcggaaccgc gttcatgtac
     2941 caccccacgg ccggccactg ggtgctcggc gagatcctct cgtcggtcac cggcatggac
     3001 cacaccgatg ccatcgagga gctggtcacc gcgccgctcg ggctgccgcg gctgctcggc
     3061 atcgccctcg accagcaaga cggcatccag gaggcggtcg gcatcggagt gccgccgacg
     3121 cccgaggaga tggaggaggc ctacggcgtc aaggtcgaca tcgccgcgct gatcccgccc
     3181 gacgtcgccc tcggtgcgct cacgacgctc aaccaccccg aggcccgggt gatcggcgtc
     3241 cccggcggcg gagggatcat gcgggccgcc gacctcgccc tgct
//
LOCUS       DATMFS010000386         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_245067, whole genome shotgun sequence.
ACCESSION   DATMFS010000386 DATMFS010000000
VERSION     DATMFS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245067"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VNQ33_07130"
     CDS             <1..457
                     /locus_tag="VNQ33_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="activates fatty acids by binding to coenzyme A; in
                     Mycobacterium may be involved in virulence; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWJ97917.1"
                     /translation="GYHGDPERSAETFVDLHGQRWAIPGDLGRVEADGSITLLGRGAS
                     SINSGGEKVFPEEVERVLKAHPDVFDAVVVGTPDDRFGERVAAVVAHRPDRPVDLASL
                     EAHCREHLADFKVPRRVVIVDEVLRRPSGKADLDWAAATVLAGHAAEAR"
     gene            complement(491..1723)
                     /locus_tag="VNQ33_07135"
     CDS             complement(491..1723)
                     /locus_tag="VNQ33_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97918.1"
                     /translation="MKVSELADELEVPTSTVLAQCQRFGIDASWAGAELSGADVVVLR
                     SELASGAPIDLTPADPVEEPVAVAAGSASPAPRPSMPAAGAEAPPTDPAPPDGSTGDP
                     ADLPPTAVGSMPDVAAEVTPDAEPVASAPTGPGGHQGRAGGPLTPDGSRPVAPRTPPA
                     APRFDRAVRGSVIALVVAAGAYAGSNVVDLAVVVALLWLVAAVALVVAVIDAIRGRRH
                     ALTHPDRVRGAWLATFALLFAVAGIIGLTASVLAVTADDPADAPLDLSDLGSVQVARW
                     GYQRTSLLAGNGWRQVARDSGSCWRHEDRRNERAEDRVEATKANVSDTCQQGHTLEVV
                     KVFAYDRDADAPYPGADKILIAAQEKCADVFAQVQEELSSVKTDVEYPTEVGWGDADH
                     DVACTVSTDAERSGKLAP"
     gene            1919..>2125
                     /locus_tag="VNQ33_07140"
     CDS             1919..>2125
                     /locus_tag="VNQ33_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97919.1"
                     /translation="MAFNIAEVHEAVAAANPDRPAIVWGDRRWTYRELTDRTRRFANA
                     LLERGIGVHRERGELAGHESGQDHV"
BASE COUNT          275 a          794 c          772 g          284 t
ORIGIN      
        1 cggctaccac ggcgaccccg agcgcagtgc ggagacgttc gtcgacctgc acggccagcg
       61 ctgggcgatc cccggcgacc ttggccgcgt cgaggccgac gggtccatca cgctgctcgg
      121 ccggggcgcg tcctcgatca actcgggcgg cgagaaggtg ttccccgaag aggtcgagcg
      181 ggtgctgaag gctcaccccg acgtcttcga cgccgtcgtc gtcggcacgc ccgacgaccg
      241 gttcggcgag cgggtcgccg ccgtcgtcgc ccaccgcccc gaccgcccgg tcgacctggc
      301 ctcgctcgag gcccactgcc gcgagcacct ggcggacttc aaggtccccc ggcgcgtcgt
      361 catcgtcgac gaggtgctgc gccggccctc cggaaaggcc gatctggact gggctgccgc
      421 gaccgtgctg gccgggcacg ccgccgaggc gcgctgagcg ggtcctcgct ggcgcggcct
      481 gcgtcggagg tcagggcgcg agcttgccgc tgcgctcggc gtccgtggag acggtgcagg
      541 ccacatcgtg gtcggcatcg ccccaaccca cctcggtcgg gtactcgacg tccgtcttca
      601 ccgaggacag ctcctcttgg acctgggcga acacgtccgc gcacttctcc tgtgcggcga
      661 tgaggatctt gtcggcgccg gggtacggcg cgtcggcgtc acggtcgtag gcgaagacct
      721 tcacgacctc cagggtgtgc ccttgctggc aggtgtcgct cacgttcgcc ttggtggcct
      781 ccacccgatc ctcggcgcgc tcgttgcggc ggtcctcgtg ccgccagcag ctgccgctgt
      841 cgcgcgccac ctgacgccag ccgttgccgg cgaggagcga cgtccgctgg tagccccagc
      901 gcgccacctg caccgagccc agatcggaca gatccagtgg agcgtcggcg ggatcgtcgg
      961 cggtgacggc gaggacggag gccgtgaggc cgatgatgcc ggcgacggcg aacagcaggg
     1021 cgaacgtggc cagccaggcg ccgcgcacgc ggtcggggtg ggtcagtgcg tggcgtcggc
     1081 cccggatcgc gtcgatcacc gcgaccacga gcgccaccgc cgccacgagc cacagcaggg
     1141 ccacgaccac ggccaggtcg acgacgttgc tgccggcgta cgcgccggcg gcgaccacca
     1201 gggcgatgac cgacccccgg accgcccggt cgaagcgggg cgcggccgga ggggtgcgcg
     1261 gcgcgacggg gcgcgacccg tcgggtgtga gcggcccgcc cgcacggcct tggtggcctc
     1321 ccggccccgt tggcgccgag gcgaccggct cggcatcggg ggtgacctcg gccgcgacgt
     1381 cgggcatcga acccaccgcc gtgggcggga ggtcagccgg gtctccggtg ctgccgtccg
     1441 ggggcgctgg gtctgtgggc ggggcctcgg ctccagcggc aggcatcgac ggacgagggg
     1501 ccggcgacgc agaccccgcg gcaacggcca ccggctcctc caccggatcg gcaggcgtca
     1561 agtcgatggg agcaccgctc gccagctcgg accgcaggac cacgacgtcg gctccgctca
     1621 gctcggcacc ggcccagctc gcgtcgatgc cgaaccgctg gcactgggcc agcaccgtcg
     1681 aggtcgggac ctccagctcg tccgcgagct ccgagacctt catcgagcag ctccgtcgtc
     1741 tccggcacgt ctctcgtccc acgtcgtggt gtgcatggtc cctccccagc gctgggcgca
     1801 ccctcgttca cccagcgcgt ggatcgtagt tcgcgagggt gcgctcagcg gcacctgccg
     1861 acgccctcgg tgccagaggt agcctccccg gaccgtccgt caccctcagg gagtcccgat
     1921 ggcgttcaac atcgccgagg tccacgaagc cgtcgccgcc gccaacccgg accggccggc
     1981 catcgtctgg ggcgatcgac gctggaccta ccgggagctg accgaccgca cccgccgctt
     2041 cgcgaacgcc ttgctcgagc ggggcatcgg ggtgcaccgc gagcgcggtg agctggccgg
     2101 ccacgagtcc ggccaggatc acgtc
//
LOCUS       DATMFS010000387         3990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_293045, whole genome shotgun sequence.
ACCESSION   DATMFS010000387 DATMFS010000000
VERSION     DATMFS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293045"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VNQ33_07145"
     CDS             <1..913
                     /locus_tag="VNQ33_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWJ97920.1"
                     /translation="TLQRFAPKLVLAFDPDAAGQAAADRVYEWERKHEVEVAVAALPP
                     GGDPADLARSDPDALRASIENAQPFLGFRIDRILAAADLRTPEGRGKVAEVALAAVAE
                     HPNEFVRDQYVMTIAEQCRLDPDRLRASLRSGGTRLRVRVAPEPKRPARAETPQTEAL
                     RLAIADPATMLALLHPALFEEPLQRAALAALDAADGDLHRAVGDADPLVGELLSRLAV
                     EETPADPVDVRRFLLRDLGARVLADLQRDLNGWTGDFRSDDYRLIVEQHAWLKHRLES
                     IGPDAPPDPIGEDELLAWLAQRGEGTA"
     gene            910..2226
                     /gene="rpoD"
                     /locus_tag="VNQ33_07150"
     CDS             910..2226
                     /gene="rpoD"
                     /locus_tag="VNQ33_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWJ97921.1"
                     /translation="MNDMAAAPTSDPSAWDELIQRAKDDGSLTVTQAEVLEVVDINVD
                     RLDQEIDAIHKLLGEHGITLDVEIDAATLAADLEAQAEHDAVVHVNEVLADPLGGIDL
                     AADEDGSRRRLRLVKTPRVNADRSGSGSADPVRVYLKEIGRVPLLTGMEEVSLAKRIE
                     AGGQAAVRLAELEAAGRIDELDVAARRRLERTSRDGERAKSDLIQANLRLVVSIAKRY
                     VGRGMHLLDLIQEGNLGLMRAVEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRI
                     PVHMVESINKVHRIQRQMMQELEREPTIDELAEKVDMTADRVREIMRISQDPLSLDSP
                     VGETDDSQLSDFIEDTQADAPAEMAARNMLGKAVVEALSELSDREKEVVRLRFGLDDG
                     QARTLEEVGKEFGVTRERIRQIEAKTLAKLRNPNRSQKLRDYLDSD"
     gene            2288..2363
                     /locus_tag="VNQ33_07155"
     tRNA            2288..2363
                     /locus_tag="VNQ33_07155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2321..2323,aa:Asn,seq:gtt)
     gene            2478..3098
                     /locus_tag="VNQ33_07160"
     CDS             2478..3098
                     /locus_tag="VNQ33_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWJ97922.1"
                     /translation="MAPRRMFPLGSVLLPGMVLPLHVFEERYRVLVRDCMAGDRRFGV
                     TLIERGSEVGGGDIRAMAGCMAEIVQADELPDGRWSVVAVGTDRIRVARWLPDAPYPI
                     AEVEDWPDVPSADPEAAVASYEARVAQLRRVLALAVELGAEADPTVPLADDAEVGSFQ
                     ISVLSPIGALDRQRLLTAPGVDDRLTMLDELLADQELMIRARLDGF"
     gene            3377..>3990
                     /locus_tag="VNQ33_07165"
     CDS             3377..>3990
                     /locus_tag="VNQ33_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97923.1"
                     /translation="MVEIGSRDPDDYIYLPIDQALEAVEALQLAIPEVKTRHDVSILQ
                     HRAITTAEAALGPDHPVLRSLDFRVLAPSVLSVFWRGSQVSTLEITLALNELRLALTA
                     RLARPLETSNLNQLLSSNPRIPERGEVDALLTGIGRLEAEVRLASEISESERQDLLRV
                     LTQARNILDHVSDPYVARAEVERLVGRAVASSAHRTPAQPLWRKV"
BASE COUNT          639 a         1383 c         1364 g          604 t
ORIGIN      
        1 cacgctccag cgcttcgccc cgaagctggt cttggccttc gaccccgatg ccgccggcca
       61 ggccgcggcc gaccgcgtct acgagtggga gcgcaagcac gaggtcgagg tggccgtcgc
      121 cgcgctcccg cccggcggcg atcctgccga cctggcccgc tcggatcccg atgcgctgcg
      181 cgcgtcgatc gagaacgccc agccgttcct cggcttccgc atcgaccgca tcctcgccgc
      241 cgccgacctg cgcacccccg agggccgggg caaggtcgcg gaggtcgcgc tcgccgccgt
      301 cgccgagcac ccgaacgagt tcgtgcggga ccagtacgtg atgacgatcg ccgagcagtg
      361 ccgcctcgac cccgaccgcc tgcgcgcctc gctgcgctcg ggtggaaccc gcctccgggt
      421 tcgggtggcg ccggagccga agcgcccggc ccgcgccgag acgccccaga ccgaagcgct
      481 ccggctggcc atcgccgacc ctgcgaccat gctggcgctc ttgcacccgg cgctgttcga
      541 ggagcctctg cagcgcgccg cgctcgccgc gctcgatgcc gccgacgggg acctgcaccg
      601 agccgtgggc gacgccgatc cgcttgtcgg cgagctgctg tcccgcctcg ccgtggagga
      661 gacgccggcg gacccggtcg acgtgcggcg cttcttgctg cgcgatctcg gggctcgggt
      721 cctggctgac ctgcagcgcg acctcaacgg gtggacgggt gacttccgca gcgacgacta
      781 ccgcctgatc gtcgagcagc acgcatggct gaagcaccgg ctggagtcca tcggaccgga
      841 cgctccgccc gatccgatcg gtgaagatga gttgctagcg tggctcgcac agagggggga
      901 gggaaccgca tgaacgacat ggctgcagcg cccacctccg atccctccgc atgggatgaa
      961 ctgatccaac gggcgaaaga cgacggctcc ctcacggtga cccaggccga ggtgctcgag
     1021 gtcgtcgaca tcaacgtgga tcgacttgat caggagatcg acgccatcca caagctgctc
     1081 ggcgagcacg gcatcacgct ggacgtcgag atcgacgccg ccaccctcgc ggccgacctc
     1141 gaggcccaag ccgagcacga tgccgtcgtg cacgtgaacg aggtgctcgc ggatccgctc
     1201 ggcggcatcg acctcgcggc cgacgaggac ggctcgcgcc gccggctgcg gctggtgaag
     1261 acgccgcgcg tcaacgcgga ccgctcgggc tcgggcagcg ccgaccccgt ccgggtgtac
     1321 ctcaaggaga tcggccgggt cccgctcctc accggcatgg aggaggtctc gctcgccaag
     1381 cgcatcgaag cgggaggcca agccgccgtc cgactcgccg agctggaggc cgccggacgc
     1441 atcgacgagc tcgacgtggc ggcccgccgc cggctcgagc gcacgtcgcg tgacggcgag
     1501 cgggccaaga gcgacctgat ccaggcgaac ctgcgcctcg tcgtgtcgat cgccaagcgc
     1561 tacgtcgggc gcgggatgca cctgctggac ctgatccagg agggcaacct cgggctcatg
     1621 cgtgcggtcg agaagttcga ctacacgaag ggcttcaagt tctccacgta cgccacctgg
     1681 tggatccgac aggccatcac ccgggccatc gccgaccagg cccgcaccat ccgcatcccg
     1741 gtgcacatgg tggagtccat caacaaggtc caccggatcc agcgccagat gatgcaggag
     1801 ctcgagcgcg agcccaccat cgacgagctg gccgagaagg tcgacatgac cgcggaccgg
     1861 gtgcgggaga tcatgcggat ctcccaggat ccgctgtcgc tcgactcgcc ggtgggcgag
     1921 accgacgatt cgcagctctc cgacttcatc gaggacaccc aggccgacgc ccctgccgag
     1981 atggctgccc gcaacatgct gggcaaggcc gtcgtggagg cgctctcgga gctgagcgac
     2041 cgggagaagg aggtcgtgcg gctgcgcttc ggcctcgatg acggccaggc tcgcaccctc
     2101 gaagaggtgg gcaaggagtt cggcgtcacc cgggagcgga tccgccagat cgaggccaag
     2161 accctcgcca agctccgcaa ccccaaccgc agccagaagc tgcgcgacta cctcgacagc
     2221 gactgatccg ctcccggaac cggggttcgt cggtccgggg tctcgctgct aggttgaccc
     2281 ctcgccttcg gaggtagctc agctggcaga gcagctgact gttaatcagc gggtcgtggg
     2341 ttcgagcccc accctccgag ccaccgagca acgatggaag ccggccccga cggggccggc
     2401 ttccaccgtt cgacaacagc agcggatgtc gtgcgctcgc cgcaccaggc ggggtgggtg
     2461 tggatagggt ccgggcgatg gcgcctcgtc gcatgttccc gctgggttcg gtgctcctgc
     2521 cggggatggt gctgccgctg cacgtgttcg aggagcggta ccgggtgctg gtgcgggact
     2581 gcatggccgg cgaccggcgg ttcggcgtga cgctgatcga gcgggggagc gaggtcggcg
     2641 gcggagacat ccgggcgatg gccgggtgca tggccgagat cgtccaagcc gacgagctgc
     2701 cggacggacg gtggagcgtc gtcgccgtcg ggacggaccg catccgggtg gcgcgctggc
     2761 tgcccgatgc cccgtacccg atcgccgagg tcgaggactg gcccgatgtg ccgtctgcgg
     2821 acccggaggc cgcggtggcg tcgtacgagg ctcgggtcgc ccagctccga agggtcctcg
     2881 ccctagccgt cgagctgggt gccgaggccg atcccacggt cccgctggcc gacgatgccg
     2941 aggtcggcag cttccagatc agcgtcctca gcccgatcgg cgccctcgac cgccagcgcc
     3001 tcctcaccgc tcccggcgtc gacgaccgcc tgacgatgct cgacgagctc ctggccgacc
     3061 aggagctcat gatccgagcc cgcctcgacg gcttctgagc cacggcggac cgagtgcgaa
     3121 cgggcacgcc ctgcacttcg accgggacga cgccggcacc gcctgcgaga cctgagcagc
     3181 gaggctcctc gaccggtctg ccaagatcgg caccggggcg gtagctcaat ggtgagagca
     3241 ggggactcat aacctgctcc cgctgagcat tgtgcagtca ggaatccgcc tgactgggtg
     3301 gtagcccgac gtgtgcgttg tagcccaact ctcctgctgt cgaggagcta cagctaggtc
     3361 aactacggtc ccccatatgg tggaaattgg cagtagggat ccggacgact acatctacct
     3421 tccgattgac caggccctgg aggcggtgga agccctccag ctcgcgatcc cggaagtcaa
     3481 gacgcgccac gacgtatcga tcctgcagca tcgagcgatc acgacggctg aagcggcgtt
     3541 ggggcctgat catccggtcc tcagatcgct tgattttcgc gtgctggcac cgtcggtctt
     3601 gtcggtcttt tggcggggta gccaggtgtc cacgcttgag atcactcttg cactcaacga
     3661 gttgagactt gcgttgaccg cacgcctggc ccgtccgttg gagacttcca acctgaatca
     3721 gctcctctcc tcgaacccgc gtattcccga gcgcggcgag gtcgacgctc tgctcacggg
     3781 gatcgggcgg ttggaggcgg aggtcagact cgcctccgag atcagtgaat cggagcgcca
     3841 ggatctgctc cgtgtactaa cgcaggcacg gaacattctt gatcatgtct ccgatcccta
     3901 cgtagcgcgt gcagaggtcg aacgtctcgt tggccgagct gtcgcgagtt cggcgcaccg
     3961 gacgccggct caaccgcttt ggcgaaaggt
//
LOCUS       DATMFS010000388         5128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_267203, whole genome shotgun sequence.
ACCESSION   DATMFS010000388 DATMFS010000000
VERSION     DATMFS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267203"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VNQ33_07170"
     CDS             complement(<1..1118)
                     /locus_tag="VNQ33_07170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWJ97924.1"
                     /translation="MRRWIAGAVLAGALAGCSSSAGDGTPDRSTTRLAPAATSTTAPA
                     APAAPASAARVPGDEWAVEQPDAEGMDAAKLEEARAYAFADGRNTQGVVVVRDGAIVS
                     EWYADGADADSWTASWSVAKSFSSAVAGIAVEEGAIGRVEDSAADYLPEWKGTDKEAI
                     TVEDILHMQSGLKWDEDYRPSSLETSDVVQMGLSPNELAYAAGRPVAHPPGTVFNYSS
                     GDAMVLAGIIEGATGEPMRDYAKEHLFDPIGVERADWWQDADGNTLGYCCLDSTARGF
                     ARLGLLYLRDGRWGDQQVIPAAWVEASIANASPAADDPGYGYMWWLLSDVEGVPDDTY
                     AAQGHDGQFIYVIPSLDLVVVRTGTYVKDPGPAVADPNL"
     gene            1214..2473
                     /locus_tag="VNQ33_07175"
     CDS             1214..2473
                     /locus_tag="VNQ33_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWJ97925.1"
                     /translation="MRRTTTRPTRPARRHRALLGPIAAVLLLAACAGSSDATSGAGRE
                     TAPTGSATTEPKTSAPGPEHYDGTDDDFYVVPDPLPKGEPGQLIRVQDLGEEDGQHTV
                     RVMYHSRDAEDRDRAVTGIISYPTAAAPEGGWPVTSQANGTVGVAGQCALSRQGNPAG
                     DWGVEGVHAVTDYIGMGPVGEIHPYLSGPSEGNSVIDAVRAARNLPEAHAGTRWFSIG
                     HSQGGHGALFAGERAAGYAPELDLLGTVSLAPATELTKTYGPADEVVSRIVGVMMLYG
                     AHSEHPEIHFDDYAGAELRAVEDVFEDGCLDDIISATAGIPGDAFYAHDPIETEPAKG
                     LLHLNQPGLVATDSPLFLAQGTDDERVNEQRTQDFFERVCKLGQVTDLLVVEGANHGG
                     IVPAAAPAAAAWMNDRLAGKPAPDSCP"
     gene            2527..3105
                     /locus_tag="VNQ33_07180"
     CDS             2527..3105
                     /locus_tag="VNQ33_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ97926.1"
                     /translation="MADDQRRRIMTAMAEVMAAQGYASTTIADVIGRAGVSRETYYQQ
                     FRSKLECFLAAFDAASAALLAPIAGRITDATEGDAPIGPADRLEVFDELFGVYLDAIT
                     SHPELARVFLIEVYAAGPEAMERRARLQATITDAIAVLLGLGDDRVRFACQALVAAIG
                     ALVTPLLATGDLDGIRALREPIVDLAARSLLA"
     gene            3222..4931
                     /gene="sulP"
                     /locus_tag="VNQ33_07185"
     CDS             3222..4931
                     /gene="sulP"
                     /locus_tag="VNQ33_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HWJ97927.1"
                     /translation="MTSVERFVPGLAAARSYERSWLGHDLVAGLVLTALLVPQGMAYA
                     ELAGLPAITGLYTSIMCLLAYAVFGPSRILVLGPDSSLGPMIAATILPLAGADGDPAK
                     AIALASMLAVLVAVFMIGAGVAKLGFVADLFSKPTQIGYMNGLALTILVGQLPKLFGF
                     SVDADGFLEELRGFVRGVADGDTLGAALAVGASGLAIILVLNKVAPKIPGVLVAVVGA
                     IAVTVVFDLGGEGVPLVGQLPQGFPPFTIPSVGWGDLTLLVAGALGITLVSLTDTIST
                     SSAFAARTGQEVDANQEMIGIGTANLAAGLFQGFPVSTSGSRTAVAEQAGARSQLTGV
                     VGAAAIAAMLLFVPGLMKDLPQPVLASVVIAASLSLADIAATRRLWHQRRMEFGVSMV
                     AFVGVALLGVLPGIGIAVIISILNVFRRMWRPYRTVLAEVDGLDGYHDVHEHPSGRRI
                     PGLSLFRFDAPLVFANARTFRDEIRRLAAAEPKPIWILVAAEPITDVDTTAADMLEDL
                     DEELNQAGISLVFAEMKSPVQDKIDRYELTRTIDPAHFFPTLDDAVEAFRSQHGDTPE
                     TNA"
     gene            4928..>5128
                     /locus_tag="VNQ33_07190"
     CDS             4928..>5128
                     /locus_tag="VNQ33_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97928.1"
                     /translation="MTPLPDTDTAPAVPPVHHVAPPDRAAAVGALVLAAAALVVGAVS
                     LAGDLGRALLAPAALLVVVGAAW"
BASE COUNT          677 a         1964 c         1747 g          740 t
ORIGIN      
        1 aggttcggat ccgccacggc cggaccgggg tccttcacgt aggtgccggt gcggaccacc
       61 accaggtcga ggctcgggat cacgtagatg aactgaccgt cgtggccctg ggcggcgtag
      121 gtgtcgtcgg ggacgccctc gacgtcggac aagagccacc acatgtaccc gtagcccggg
      181 tcgtcggccg ccggcgacgc gttggcgatc gacgcctcca cccaggcggc ggggatcacc
      241 tgctggtcgc cccacctccc gtcgcgcagg tacaagaggc cgaggcgggc gaagccccgg
      301 gccgtcgagt ccaagcagca gtagccgagc gtgttcccgt cggcgtcttg ccaccagtcc
      361 gcccgctcca ccccgatggg gtcgaagagg tgctccttcg cgtagtcccg catcggctcg
      421 ccggtggccc cctcgatgat gccggcgagg accatggcgt cgccgctcga gtagttgaag
      481 acggtccccg gcgggtgcgc cacaggccgc ccggccgcgt aggccagctc gttcgggctc
      541 agccccatct gcaccacgtc cgaggtctcg agcgacgacg gccggtagtc ctcgtcccac
      601 ttcaggcccg actgcatgtg gaggatgtcc tcgacggtga tggcctcctt gtcggtcccc
      661 ttccactcgg gcaggtagtc cgcggccgaa tcctcgacgc ggccgatggc cccctcctcg
      721 acggcgatgc cggcgaccgc gctgctgaag ctcttggcca ccgaccagct cgcggtccac
      781 gagtcggcat cggcgccgtc ggcgtaccac tccgagacga tggcgccgtc gcgcacgacc
      841 accacgccct gggtgttgcg gccatcggcg aacgcgtacg cccgcgcctc ctccagcttc
      901 gccgcgtcca tcccctcggc gtcgggctgc tccacggccc actcgtcgcc gggcacgcgg
      961 gcggccgagg cgggcgcggc gggcgcggcg ggcgcggtcg tgctcgtcgc tgcgggcgcc
     1021 agacgggtgg tggaccggtc cggcgtcccg tcgcccgcgc tgctgctgca ccccgcgagc
     1081 gccccggcca gcacggcccc tgcgatccat cgacgcatcc gccggttccc catccgacca
     1141 gtcaaccact cgtcggggcg gtccgcgggc gatccacgaa catcaacgtt cttcgatccg
     1201 ctaccgtccg gcgatgcgcc ggaccaccac tcgccccacc cgccccgccc gccgacaccg
     1261 ggcgctcctc ggtccgatcg ccgcagtcct cctgctcgcc gcctgcgccg ggtcatccga
     1321 cgccaccagc ggcgccggcc gcgagaccgc gccgacgggc tccgccacga ccgagccgaa
     1381 gacgagcgca cccggtcccg agcactacga cgggaccgac gacgacttct acgtcgtgcc
     1441 cgatccgctg ccgaagggcg agccgggcca gctcatccgg gtgcaggacc tcggggagga
     1501 ggacggccag cacacggtgc gggtcatgta ccactcgcgt gacgcggagg accgcgaccg
     1561 agccgtcacc ggcatcatct cctacccgac cgccgcggcg cccgaaggcg ggtggccggt
     1621 cacgtcgcaa gccaacggca cggtcggcgt cgctggccag tgcgccctga gccgccaggg
     1681 gaaccccgcc ggcgactggg gcgtcgaggg cgtgcacgcc gtgaccgact acatcggcat
     1741 gggtccggtc ggcgagatcc atccctacct gtccgggccg agcgagggca acagcgtgat
     1801 cgacgccgtc cgcgccgccc ggaacctgcc cgaggcgcac gccggcacgc ggtggttctc
     1861 gatcggccac tcgcaggggg gccacggcgc gctgttcgcc ggcgagcggg ccgccggcta
     1921 cgcgccggag ctggacctgc tcggcaccgt gtcgctcgcc ccagcgaccg agctgaccaa
     1981 gacctacggc cccgccgacg aggtggtgtc ccgcatcgtc ggcgtgatga tgctctacgg
     2041 cgcccacagc gaacaccccg agatccactt cgacgactac gccggcgcgg agctgcgggc
     2101 cgtcgaggac gtgttcgagg acggctgctt ggacgacatc atcagcgcca cggccggcat
     2161 cccgggtgac gccttctacg cccacgaccc gatcgagacc gagccggcca aggggctgct
     2221 ccacctcaac cagccgggtc tcgtggccac cgactcgccg ctcttcctcg cccagggcac
     2281 cgacgacgag cgggtcaacg agcaacgaac gcaggacttc ttcgagcggg tgtgcaagct
     2341 cggccaggtg accgacctgc tcgtggtcga gggcgcgaac cacgggggca tcgtccccgc
     2401 ggccgccccc gcagccgccg cgtggatgaa cgaccgcctg gccgggaagc cggctccgga
     2461 ctcgtgcccg tgagcgatca cgtctcgcgc ctcccccggg gtcgccacgg gctgacgcgc
     2521 gcagaggtgg ccgacgacca gcgccgccgg atcatgacgg cgatggccga ggtcatggct
     2581 gcgcagggct acgcgtcgac caccatcgcc gacgtcatcg ggcgcgccgg cgtgtcgcgc
     2641 gagacctact accagcagtt ccgctcgaag ctcgagtgct tcctcgccgc gttcgacgcg
     2701 gcatccgcag cgctgctcgc cccgatcgcc gggcggatca ccgatgccac cgaaggcgat
     2761 gctcccatcg gtcccgccga ccgcctcgag gtcttcgacg agctgttcgg cgtctacctc
     2821 gacgccatca cctcccaccc ggagctcgcc cgggtcttcc tgatcgaggt ctacgccgcc
     2881 gggcccgagg cgatggagcg tcgggcccgg ctccaagcga ccatcaccga tgcgatcgcc
     2941 gtgctgctcg ggctcggcga cgaccgcgtg cggttcgcgt gccaggccct cgtcgcggcg
     3001 atcggcgccc tcgtcacgcc gctgctggca acgggcgacc tcgacggcat ccgcgccctg
     3061 cgggaaccga tcgtggacct cgccgcccgc tccctcctcg cctgacccgt cgttcccggc
     3121 accgagcggt tgcaccgtcg ggggaacgca ggtaggaaca cgtcgaaggc cgcgtcggag
     3181 cggtagcgac cgcaggtggg gtgcaggatc gagatggtgg ggtgacctcg gtcgagcggt
     3241 tcgtccccgg cctcgccgcc gcccggtcct acgagcggtc ctggctcgga cacgacctcg
     3301 tcgccggcct ggtcctcacc gccctcctcg tgccgcaggg catggcgtac gcggagctgg
     3361 cggggctccc ggccatcacc ggcctctaca cgtcgatcat gtgcctgctc gcctacgcgg
     3421 tgttcggccc gtcccggatc ttggtgctcg ggccggactc gtcgctcggg ccgatgatcg
     3481 cggcgacgat cctcccgctg gcgggggccg acggggaccc ggccaaggcc atcgccctcg
     3541 catcgatgct ggccgtcttg gtggccgtct tcatgatcgg tgccggcgtc gccaagctcg
     3601 ggttcgtcgc cgacctgttc tccaagccca cccagatcgg gtacatgaac ggcctggccc
     3661 tgacgatcct cgtcggccag ctcccgaagc tgttcgggtt ctcggtcgac gctgacggct
     3721 tcctcgagga gctccgcggc ttcgtccggg gcgtcgccga cggggacacc ctgggcgctg
     3781 ccctcgccgt cggcgccagt gggctggcga tcatcttggt cctcaacaag gtggcgccga
     3841 agatccccgg cgtgctggtc gccgtggtgg gcgccatcgc cgtcaccgtg gtgttcgacc
     3901 tcggcggcga gggcgtgccg ctcgtcggcc agctgcccca ggggttcccg cccttcacca
     3961 tcccgagcgt cggctggggc gacctcaccc tgctcgtggc cggcgccctg ggcatcacgc
     4021 tcgtgtcgct caccgacacc atctccacgt cgtcggcgtt cgcggcccga accggccagg
     4081 aggtcgacgc caaccaggag atgatcggca tcggcacggc caacctggcc gccgggctgt
     4141 tccaggggtt cccggtgagc accagcggtt cgcgcacggc ggtggcggag caggccgggg
     4201 cgcgctccca gctcaccggg gtggtcggcg ccgcggccat cgccgccatg ttgctgttcg
     4261 tccccgggct gatgaaggac ctgccccagc cggtgctcgc ctcggtcgtg atcgcggcgt
     4321 cgctgtcgct ggccgacatc gccgccacca ggcggctgtg gcaccagcgc cggatggagt
     4381 tcggcgtgtc gatggtggcg ttcgtcggtg tggcgctgct cggtgtcctg ccgggcatcg
     4441 ggatcgccgt gatcatctcg atcctcaacg tgttccgccg catgtggcgg ccgtaccgca
     4501 cggtgctggc cgaagtcgac gggctcgacg gctaccacga cgtccacgag cacccgagcg
     4561 gccggcggat ccccggcctg tcgctgttcc ggttcgacgc cccgctcgtc ttcgccaacg
     4621 cccggacgtt ccgcgacgag atccgacggc tggccgcggc ggaaccgaag ccgatctgga
     4681 tcctcgtggc cgccgagccc atcaccgacg tcgacaccac cgccgccgac atgctcgagg
     4741 acctcgatga ggaactgaac caggcgggca tctcactggt gttcgccgag atgaagtcgc
     4801 cggtgcagga caagatcgac cgctacgagc tgacccgcac gatcgacccc gcccacttct
     4861 tccccacgct cgacgacgcc gtcgaggcgt tccgctccca gcacggcgac accccggaga
     4921 ccaacgcatg acacccctgc ccgacaccga caccgctcct gcggtcccgc ccgtccacca
     4981 cgtggccccg ccggaccggg ccgccgcggt gggcgcgctg gtcctcgccg ccgcggcgct
     5041 ggtggtgggt gcggtcagcc tcgcgggcga cctcggccgg gccctgctcg caccagcagc
     5101 cctcctcgtg gtggtcggcg cggcgtgg
//
LOCUS       DATMFS010000389         4994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_59710, whole genome shotgun sequence.
ACCESSION   DATMFS010000389 DATMFS010000000
VERSION     DATMFS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59710"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VNQ33_07195"
     CDS             <1..854
                     /locus_tag="VNQ33_07195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWJ97929.1"
                     /translation="MAVSPLQALHAAGFEIGLVITGPPKRRGRGGTSTPTPVGLEALA
                     LGLSVSHDVADAATAGADLGVVVAFGQIIGRPVLEALPMVNVHFSLLPRWRGAAPVER
                     AILAGDERTGVCVMAVEEGLDTGGVYARSELTIRQTNTAAELGAQLTELGSTLLVETL
                     RSGLAAPEPQVGEPTYAHKLTSADRQLDWTRSADELRRVVRVGGAWTTFRGRRLKVLD
                     AVIDGAGGEPAGAPAGVLDGNRVATGAGALRLVTVQPEGKGPVAAADWLHGARPAPGE
                     RLGEAAS"
     gene            935..2041
                     /locus_tag="VNQ33_07200"
     CDS             935..2041
                     /locus_tag="VNQ33_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWJ97930.1"
                     /translation="MAVDALVRIERDGAYANILLPKLLSRSALAERDRAFATQLVYGT
                     VRAQRSCDWFISRFALGDLDETVRAALRVGTFQLRFLGTPPHAAVSATVEVVPRKARG
                     LVNAVLRKVADADDAWPSDAVRLSYPDWVVEHLTADLGSERALAALTSMNGAATTTER
                     DDGYTQDPASRWVVDAVGAQPGERVLDLCAAPGGKATALAGDGARVVAADVRPRRVGL
                     IAGNADRVGLTPEQLQAVAADGTRPPFADESFDRVLVDAPCSGLGSLRRRPDARWRVD
                     ADAPGRLSRLQARLVEAAAPLVAPGGSLTYSVCTLTTEETTGVAEAFAAAHPDWPVVE
                     APGDPWIPWGTGALLLPQTEETDGMAVFTWRRPD"
     gene            2055..2558
                     /locus_tag="VNQ33_07205"
     CDS             2055..2558
                     /locus_tag="VNQ33_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWJ97931.1"
                     /translation="MTEAALSGGPPPGRWRARIVVVSDGVADGTRDDRGGPALREALE
                     AVGATVEAVVTTPDGVEAVADTLLAQCAGFDGLVVTTGGTGFGPRDLTPEGTRRVLDR
                     EAPGLAEAMRLVNPLGRLSRGIAGTRGGALVLNVPGSPKGAVECLGAVLDVVPHALAL
                     LAGDRPH"
     regulatory      2673..2799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2844..3905
                     /gene="ribD"
                     /locus_tag="VNQ33_07210"
     CDS             2844..3905
                     /gene="ribD"
                     /locus_tag="VNQ33_07210"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWJ97932.1"
                     /translation="MDDAARMQQAIEAAAAIRAQTSPNPWVGCVIESPAGEVSTGATE
                     PPGGRHAEVVALDAAGDGARGATAWVTLEPCSHTGRTGPCADALIEAGVARVVVALED
                     PDPRVAGSGIERLRAAGVEVVVGTLADAVRTQLAAYLKHRSTGRPWVVLKVGASIDGR
                     TAAPDGSSQWITGGAARADAHQLRADSDAIVVGAGTVRADDPSLTVRSVEGKDPIRVV
                     LGEAPAGAKVLPALQFSGELTDLLDQLGERGVLQVLVEGGATVAGAFHRAGLVDHYVV
                     YLAPAIFGGDDARPLFAGPGAETIADVWRGAITSVTPLGGDVRIDLAPVGPSPVTGPV
                     PVVRIGDVPPVHDPASGGA"
     gene            3905..4474
                     /locus_tag="VNQ33_07215"
     CDS             3905..4474
                     /locus_tag="VNQ33_07215"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWJ97933.1"
                     /translation="MFTGIVEELGTVISREGPKLRIGATTVLTDVELGASIAVSGTCL
                     TVVAWGEDWWEADVVDETYARTCLGALQPGDPVNLERPVRLEDRLGGHLVQGHVDAVG
                     TIVSAAPDLRIRMPEDLLRYVVHKGSITIDGTSLTVAAVHGDGFSVAVIPHTAEVTTL
                     GSKGPGDPVNLELDVIAKYVESLLEGHRS"
     gene            4471..>4994
                     /gene="ribB"
                     /locus_tag="VNQ33_07220"
     CDS             4471..>4994
                     /gene="ribB"
                     /locus_tag="VNQ33_07220"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWJ97934.1"
                     /translation="MSDNPAEMPAQVGDFEVANRGPADDGPLADISEALAAYARGEIL
                     VVVDDEDRENEGDLIMAAEFVTPEKIAFFLHHTSGFICAPITSERAAELDLRPMVEHN
                     TESMRTAFLVSIDYRHGTSTGISSFDRATTVQALVDPNTRPGDFARPGHILPLEAREG
                     GVLKRAGHTEATVDL"
BASE COUNT          626 a         1942 c         1781 g          645 t
ORIGIN      
        1 cgatggccgt ctccccgttg caggcgctgc acgccgcggg cttcgagatc ggcctggtga
       61 tcaccggtcc gccgaagcgg cgcggtcgcg gcggcacgtc gaccccgacg ccggtggggc
      121 tcgaggcgct cgccctcggc ctctccgtgt cccacgacgt cgctgacgcc gcgaccgccg
      181 gtgccgacct gggcgtggtg gtggcgttcg ggcagatcat cggccgaccg gtgctcgagg
      241 ccctgcccat ggtgaacgtg cacttctcgc tgctgccccg gtggcgcggc gcggcgcccg
      301 tcgagcgggc gatcctcgcg ggcgacgagc ggacgggcgt ctgcgtgatg gcggtcgagg
      361 aggggctcga caccggtggg gtctacgccc gctcggagct cacgatccgc cagacgaaca
      421 cggcggccga gctgggcgcg cagctcaccg agctgggcag cacgctgctg gtcgagacgc
      481 tgcgatcggg tctggcggcg cccgagcccc aggtgggcga gcccacctac gcccacaagc
      541 tcacgagcgc cgaccgccag ctcgactgga cccgctcggc cgacgagctg cgccgggtgg
      601 ttcgcgtcgg cggcgcctgg acgaccttcc ggggtcgccg gctcaaggtc ctcgatgcgg
      661 tgatcgacgg cgcaggcggc gagcccgccg gagctccagc gggcgtgctc gacggcaacc
      721 gggtcgccac cggtgccggg gccctgcgcc tggtgacggt gcagcccgag ggcaaggggc
      781 cggtcgcggc ggccgactgg ctgcacggcg cccggcctgc gcccggcgag cgactcggcg
      841 aggcggcctc gtgagcggca gcggccgtcg tcgtcgcccc ggcccccgca cgccggcggg
      901 ggagccggtc ccacccgtcc ctgacgcccg gcgcgtggcg gtcgacgccc tggtccgcat
      961 cgagcgcgac ggcgcctacg ccaacatcct cctgcccaag ctcttgtcca ggagcgcact
     1021 ggctgagcgg gaccgggcct ttgccaccca gctcgtctac ggcaccgtcc gcgcccagcg
     1081 ctcgtgcgac tggttcatca gccggttcgc cctcggcgac ctcgacgaga ccgtccgcgc
     1141 tgccctccgc gtcggcacgt tccagctccg cttcttgggc acaccgccgc acgccgcggt
     1201 ctcggccacg gtcgaggtcg tgccccgcaa ggcccgaggc ctcgtcaacg ccgtgcttcg
     1261 caaggtcgcc gatgccgacg atgcctggcc ctccgatgcc gtgcggctca gctacccgga
     1321 ctgggtggtc gagcacctca cggcagacct ggggagcgag cgggcgctcg ccgcgctgac
     1381 ctccatgaac ggcgccgcca ccaccaccga gcgcgacgac ggctacaccc aggacccggc
     1441 gtcgcgctgg gtggtcgacg ccgtcggcgc gcagccgggg gagcgggtgc tggacctatg
     1501 cgccgccccg ggcggcaagg ccaccgcact ggccggcgac ggtgcccgcg tcgtcgccgc
     1561 cgacgtgcgg cctcgccgcg tcgggctgat cgccggcaac gccgatcgcg tgggcctgac
     1621 gccggagcag ctgcaggcgg tggccgccga cggcacccgg ccgccgttcg ccgacgagtc
     1681 gttcgaccgc gtgctggtcg acgcgccgtg ctcgggcctc ggctcgctcc gccgccgccc
     1741 cgatgcccgc tggcgggtcg acgccgatgc ccccgggcgt ctgtcgcgcc tccaggctcg
     1801 gctggtggag gcggcggcgc cgctcgtcgc tcccggtggc tcgctcacct actcggtgtg
     1861 caccctcacc acagaggaga ccaccggtgt cgccgaggcg ttcgccgccg cccatcctga
     1921 ctggccggtg gtggaggcgc cgggcgaccc gtggatcccg tgggggaccg gcgccctcct
     1981 gctgccccag accgaagaga ccgacggcat ggccgtgttc acctggcgcc gaccggactg
     2041 acgaggagcc caccttgacc gaagccgccc tctccggagg cccccctccc ggccgctggc
     2101 gggcccgcat cgtcgtcgtg tccgatggcg tggccgacgg cacccgggac gatcggggcg
     2161 gccccgccct ccgcgaagcg ctggaggcgg tgggcgccac ggtggaggcc gtggtgacca
     2221 cgcccgacgg tgtcgaggcg gtcgccgaca cgctgctcgc ccagtgcgcc gggttcgacg
     2281 gcctcgtggt gaccacgggt gggaccggct tcgggccccg tgacctcacg cctgagggca
     2341 cccggcgtgt cctcgaccgt gaggcgccgg ggttggccga ggccatgcgc ctcgtcaacc
     2401 cgttgggccg cctctcccgg ggcatcgccg gcacgcgcgg cggggcgctg gtgctcaacg
     2461 tcccggggtc gcccaagggt gcggtcgagt gcctcggggc ggtcctcgac gtggtcccgc
     2521 acgcgctcgc cctcctggcg ggagaccggc cgcactgacc gcgccggtcc gtctgacgtg
     2581 cccgatgacc gctcacgacc aacccggtcg ggattgtcgg tgacgagagc gcgtccgtac
     2641 actggccgca ccatcgagaa gaaccagcag acgcctcctc gcggggcagg gtgcaactcc
     2701 ctaccggcgg tacagcccgc gaacccgagc ctcacggccc gggccgatcc ggtgagatcc
     2761 cggagccgac ggtgacagtc cggatgagag cgagggcgcg ctgacgcttc gcgcgtcgcg
     2821 tgtccgtgac agaccgagac gacgtggacg acgcagcacg catgcagcag gcgatcgagg
     2881 cagcggccgc catccgcgcc cagacgtcgc cgaacccctg ggtgggatgc gtgatcgagt
     2941 ctccggcggg cgaggtgtcc accggagcga cggagccgcc cggcggccgc catgccgagg
     3001 tcgtcgccct cgatgccgct ggcgacggcg cccgcggcgc caccgcgtgg gtcaccctcg
     3061 agccctgctc gcacaccggc cgcaccggcc cctgcgccga cgcgctgatc gaggcgggcg
     3121 tcgcccgcgt cgtcgtcgcc ctcgaggacc ccgacccccg cgtggccggc tcggggatcg
     3181 agcggctccg ggccgcgggc gttgaggtcg tcgtgggcac cctcgccgac gcggttcgga
     3241 cccagctcgc cgcctacctc aagcaccgct cgaccggtcg cccgtgggtg gtgctgaagg
     3301 tcggggccag catcgacggg cgcaccgccg cgcccgacgg ctccagccag tggatcaccg
     3361 gtggcgcggc acgggccgac gcccaccagc tccgggccga cagcgacgcc atcgtcgtcg
     3421 gcgccggcac cgtgcgggcc gacgacccgt cgctcaccgt gcgcagcgtg gagggcaagg
     3481 accccatccg ggtcgtgctc ggcgaggccc ccgccggagc gaaggtgctg ccggccctcc
     3541 agttctccgg cgagctcacc gacctgctcg accagttggg cgagcgaggt gtgctccagg
     3601 tgctggtcga gggcggggcc accgtcgccg gcgcgttcca ccgggccggc ctggtcgacc
     3661 actacgtcgt gtacctggca ccggccatct tcggcggcga cgacgcccga cccctgttcg
     3721 ccggtcccgg cgccgagacc atcgccgacg tctggcgcgg cgccatcacc agcgtcaccc
     3781 cgctcggcgg cgacgtccgc atcgacctgg cccccgtcgg gccctcgccg gtcaccggcc
     3841 ccgtgcccgt ggtgcgcatc ggcgacgtcc ccccggtcca cgatcctgcc tccggaggcg
     3901 cctgatgttc accggcatcg tcgaggagct cggcaccgtc atctcccgcg aggggccgaa
     3961 gctgcgcatc ggcgccacca cggtcctcac cgacgtggag ctcggtgcgt cgatcgctgt
     4021 cagcggcacc tgcctcaccg tggtcgcctg gggcgaggac tggtgggagg ccgacgtggt
     4081 cgacgagacc tacgcccgca cctgcctggg cgctctccag cccggcgatc ccgtgaacct
     4141 ggagcgcccg gtgcgcctcg aggaccgcct cggcggccac ctcgtgcagg gccacgtcga
     4201 cgccgtcggc accatcgtct cggcggctcc ggatctacgg atccgcatgc ccgaggacct
     4261 gctccggtac gtggtgcaca agggctcgat caccatcgac ggcaccagcc tcaccgtggc
     4321 cgccgtccac ggcgacgggt tctccgtcgc cgtcatcccg cacaccgccg aggtcaccac
     4381 cttgggctcg aagggccccg gcgacccggt caacctggag ctcgacgtca tcgccaagta
     4441 cgtcgagtcc cttctggaag gacaccgctc atgagcgaca accccgccga gatgccggcc
     4501 caggtcggcg acttcgaggt cgccaaccgc ggcccggccg acgacggccc cctggccgac
     4561 atcagcgagg cgctggcggc ctacgcccgc ggcgagatcc tcgtggtcgt cgacgacgag
     4621 gaccgcgaga acgagggcga cctgatcatg gcggccgagt tcgtcacgcc ggagaagatc
     4681 gccttcttcc tccaccacac ctcgggcttc atctgcgctc ccatcaccag cgagcgggcc
     4741 gccgagctgg acctccggcc gatggtcgag cacaacaccg agagcatgcg cacggccttc
     4801 ctcgtgagca tcgactaccg ccacggcacg tccaccggca tctcgtcgtt cgaccgggcc
     4861 accacggtgc aggccctcgt cgacccgaac acgcggcccg gcgacttcgc ccgacccggc
     4921 cacatcctcc cgctggaggc ccgggaaggc ggcgtgctca agcgggccgg ccacaccgag
     4981 gccaccgtcg acct
//
LOCUS       DATMFS010000390         7076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_83691, whole genome shotgun sequence.
ACCESSION   DATMFS010000390 DATMFS010000000
VERSION     DATMFS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83691"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VNQ33_07225"
     CDS             complement(<1..1879)
                     /locus_tag="VNQ33_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HWJ97935.1"
                     /translation="MFRLDDDWVFSATDLVTALRCEYQVLHKRAEKAGLVPKIEVDDP
                     VRARAAELGLVHEREVLDRLVALDGSGTVGEAGGVVSIDQPSTSSRASLEAAHELTRA
                     ALEGGADVVYQAAFFHDGFHGLADFVVRTTGDDGSVWYEPADTKLARHARVEALLQLA
                     AYGGQLVAMGFPAPRAVHLWLGDRSVSSHRFADLQPILADRSERLRSLLARPVAIPVW
                     GDPDLRACGWCDHCAAAEQDRRDVLLVAGVRVDQKPKLAAVGIRTIDDLAAATACPPD
                     LKQETFDKIHAQAVLQAGQDATRTDDDPLGTVTAELSNPAGIALIPEPNPGDVFFDFE
                     GDPLYVEPGWPELGLEYLFGSITHDTGAPEYWALWAHDRGAEKAAVEQYIDWLVARRA
                     TPGFEGLHVYHYAPYEVTGLKRMVQRYGTRSEELDQLLVDGVFVDLYSVVRRSIRISE
                     RSYSIKRLEPLYMGDHERLSEVTGGADSIIWYGNYRMLRDQGKAAEAQSQLEQLAEYN
                     EYDCLSTLRLRDWLLELPGGHRPGPRPVEQEPARPVSGEQAAALALEQDLLRPFADKA
                     PAERTADEEAIALLAAALLFHRRESLPFWWAHFARMAATAEDWEHDGEMIVLPRGSVE
                     VV"
     gene            complement(1987..2145)
                     /locus_tag="VNQ33_07230"
     CDS             complement(1987..2145)
                     /locus_tag="VNQ33_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HWJ97936.1"
                     /translation="MNATILWVIAAILVIAGIVQLLQGQLLFGIALIVVGALVGPGGV
                     SVFRTDRR"
     gene            2222..2734
                     /locus_tag="VNQ33_07235"
     CDS             2222..2734
                     /locus_tag="VNQ33_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWJ97937.1"
                     /translation="MGTDPFDQLMTSANPAMVVVTAAAGDERAGCLVGFHAQSSMDPH
                     GYAVLLSKANHTFDVAVRATHLAVHFLTDADHEAARTFGTLTGDRVDKFARVPWRRGP
                     HGVPVLTACDHGLIGRKVSLLDDGGDHVVVTLDPVEVWSREPFTPLRLSAVSDLEPAH
                     GADEHPDHTR"
     gene            complement(2829..3755)
                     /gene="ligD"
                     /locus_tag="VNQ33_07240"
     CDS             complement(2829..3755)
                     /gene="ligD"
                     /locus_tag="VNQ33_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWJ97938.1"
                     /translation="MAPPRPPAEVSVMAASSTDRRLFGDDWVFERKLDGYRCLAWVDD
                     GGVRLRSRNGLSFDDTYPEVARALAAAARGSLLVDGEVVAMVDGQTSFEHLQQKASDP
                     SVPVQYVVFDLLWFDGNDIRPLPLEARQAALAEVLVPGGPIALGEVLTGDGEALLAAA
                     CGLGWEGLIAKRRASAYQPRRSKDWLKLKCVHQQEVVVGGFTEPKGSRTGLGALLVGV
                     YEGGELRYAGKVGTGFDTATLHALHRELHVLARPTSPFAGVVREKAVHWVEPTMVVEV
                     GFGEWTASGHLRHPRYLGKRPDKDAREVTRER"
     gene            complement(3755..4168)
                     /locus_tag="VNQ33_07245"
     CDS             complement(3755..4168)
                     /locus_tag="VNQ33_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWJ97939.1"
                     /translation="MSENLKILGEYSNAMFAGDTEAVFDFWGPDFHTHVTERVAPERV
                     GEDVRGDEQIWWRAVMAAIPDMAFTVDLLIEKDDLIVSNWSVRGTFDGEPFFDLPPTG
                     EPVHINGTAILRMADGKIVEHWGGPHCQKGLGLIV"
     gene            4770..5474
                     /locus_tag="VNQ33_07250"
     CDS             4770..5474
                     /locus_tag="VNQ33_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97940.1"
                     /translation="MPALVDRLASAATKPLTRTLTVEAVSDLADGFRRVDLRSSGVLR
                     WEPGQKVQVHVKGFEFRTYTPFAWDGERVSLLAALDATGPGTALVASLEPGTEVSVFG
                     PRRAMDLSKVAAAPILIGDETSFALGAAWDQHGSVPAAAHLYEVTHPEPSAQVCATLG
                     FDHATLVERTADEGHADRLTELAVDLVRSTPDAPLVLTGKAQTIRAIRGALKAAGLSP
                     ATRVKAHWDPNRSGLD"
     gene            complement(5741..6772)
                     /locus_tag="VNQ33_07255"
     CDS             complement(5741..6772)
                     /locus_tag="VNQ33_07255"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWJ97941.1"
                     /translation="MAVRGAVRLAGAAAIALLIGLTGPGAGAQEDPAAPAESTTPTEP
                     TTAAPPSIREALAGTSKAVTGSAAGGFGSDDTLDVVLTLENHTDAPVQLAVPRGALFA
                     TEDEGEQTAVTVGPIDEVAATVRPGVDPTITIEPGEHQIELAGFCAQHLDSGPAEVVP
                     MTWAGVAEEPLTTVLTTIAATEPDPIAAQHAVWWVTDVPVLPVPAEVEPLLDGVDTEA
                     FAANPKRVVPDERYTPAWGRDQALDPDDPFGDTPFSFDDPTGDDGAFSTSGGDDGSPG
                     MGLFLLGAVAACGVITILVARSASRKAPALVRSNDGATPPGWHPDPQQSQQLRYWDGS
                     TWTNDTRPF"
BASE COUNT         1015 a         2592 c         2511 g          958 t
ORIGIN      
        1 cgacgacctc gaccgacccc cggggcagga cgatcatctc gccgtcgtgc tcccagtcct
       61 cggctgtggc cgccatgcgg gcgaagtgcg cccaccagaa cggcagcgac tcccggcggt
      121 ggaagagcag ggcagcagcg agcaaggcga tggcctcctc gtccgccgtc cgctccgcag
      181 gcgccttgtc ggcgaacggc cggagcaggt cctgctccaa ggcgagcgct gccgcctgct
      241 cgcccgacac cggccgcgcc ggctcctgct ccaccggccg gggcccgggc cggtgcccgc
      301 ccggcagctc caggagccag tcgcgcaggc ggagggtgga gaggcagtcg tactcgttgt
      361 actcggcgag ctgttcgagc tgggactggg cctcggccgc cttgccctgg tcccgcagca
      421 tccggtagtt gccgtaccag atgatcgagt cggcgccgcc ggtgacctcg ctcagccgct
      481 cgtggtcacc catgtagagc ggttcgagcc gcttgatgct gtacgagcgc tcggagatgc
      541 ggatcgagcg ccggaccacg gagtagaggt cgacgaacac gccgtcgacg aggagctggt
      601 cgagctcctc gctgcgggtg ccgtagcgct ggaccatgcg cttgagcccg gtgacctcgt
      661 agggcgcgta gtggtagacg tgcagtccct cgaacccggg cgtggcccgc cgagccacga
      721 gccagtcgat gtactgctcc accgccgcct tctccgcccc gcggtcgtgc gcccacagcg
      781 cccagtactc cggcgctccc gtgtcgtggg tgatcgaccc gaacaggtac tccagcccca
      841 gctccggcca cccgggctcg acgtagagcg ggtcgccttc gaagtcgaag aacacgtcgc
      901 cggggttcgg ctcggggatg agggcgatgc cggccgggtt ggagagctcc gcggtgacgg
      961 tgccgagcgg atcgtcgtcg gtgcgggtgg cgtcctggcc ggcctggagc acggcttggg
     1021 cgtggatctt gtcgaacgtc tcctgcttga ggtcaggcgg gcacgccgtg gccgcggcca
     1081 ggtcgtcgat ggtgcggatg cccaccgccg ccagcttcgg cttctggtcg acccggaccc
     1141 cggcgacgag cagcacgtcg cggcggtcct gctccgcggc ggcgcagtgg tcgcaccagc
     1201 cgcacgctcg caggtcggga tccccccaca cggggatggc gacgggtcgg gcgaggagcg
     1261 agcgaagccg ctcggaccgg tcggcgagga tcggctgcag gtcggcgaag cggtgggacg
     1321 acaccgacct gtcgcccagc cagaggtgca ccgcccgggg tgcggggaac cccatcgcga
     1381 cgagctgacc gccgtaggcc gccagctgga gcagggcctc cacccgggcg tggcgggcga
     1441 gcttggtgtc ggctggctcg taccagaccg atccgtcgtc gccggtggtc cgcaccacga
     1501 agtcggccag cccatggaag ccgtcgtgga agaacgccgc ttggtagacg acatcggcgc
     1561 cgccctcgag cgcggcgcga gtcagctcgt gcgccgcctc cagcgaggcg cgagacgagg
     1621 tgctcggttg gtcgatcgac accacgccac cggcttcgcc gaccgttccc gacccgtcca
     1681 gggcgacgag tcgatcgagc acctcccgct cgtggacgag ccccagctcg gccgcccggg
     1741 cccggacggg atcgtcgacc tcgatcttcg ggacgagccc ggccttctcc gcccgcttgt
     1801 ggaggacctg gtactcgcag cgcagcgcgg tcaccaggtc ggtggcgctg aacacccagt
     1861 cgtcgtcgag gcggaacatc gcttgggagc atcccacacc cctgtgacac tcaggatgta
     1921 cgggtcagcg gggcggggag ccgcggcttc aggcggtcgg ccgacggggg cgggccgccg
     1981 ctccgctcac cggcggtcgg tgcggaacac cgacacgccc ccggggccga cgagggcacc
     2041 gacgacgatg agggcgatcc cgaagaggag ctgaccctgc aacagctgca cgatgccggc
     2101 gatcacgagg atggcggcga tgacccagag gatggttgcg ttcatgccgt ccactgccct
     2161 ccgtccagat gggcgaaacc cagatggtga gaacgtcccg gaggtgggta agagatcagc
     2221 cgtgggaacc gatccgttcg accagttgat gacctcggcc aacccggcca tggtcgtcgt
     2281 gaccgcagca gccggcgacg agcgggcggg ctgcctggtc ggcttccacg cccagtcgag
     2341 catggacccg cacggctacg ccgtcctcct ctcgaaggcg aaccacacgt tcgacgtggc
     2401 ggtccgggcg acgcacctcg ccgtccactt cctgaccgac gcggaccacg aggcggcccg
     2461 caccttcggc accctcaccg gtgaccgcgt cgacaagttc gctcgcgtcc cgtggcggcg
     2521 cggcccgcac ggcgttcccg tgctcaccgc ctgtgaccac gggctcatcg gccggaaggt
     2581 cagcctgctc gacgacgggg gcgaccacgt ggtcgtcacg ctcgacccgg tggaggtgtg
     2641 gagccgcgag cccttcaccc cgctgcggct ctcggccgtg tccgacctgg aaccggcgca
     2701 cggcgccgac gagcaccccg accacacccg ctgaccccgg gtccgcgcgc cgacggactc
     2761 gaccggagcg accggcgacg ccggatcaga cggcgacgac cgtggcgggg atgccggccg
     2821 gcacgacgtc aacgttcgcg ggtgacctcc cgggcgtcct tgtcgggccg cttccccagg
     2881 taccgggggt ggcgcaggtg cccgctggcg gtccactcgc cgaagccgac ctcgaccacc
     2941 atcgtcggct cgacccagtg cacggccttc tcgcgcacga cgccggcgaa gggcgatgtc
     3001 ggtcgcgcca gcacgtgcag ctcccggtgc agcgcatgga gcgtggcggt gtcgaacccg
     3061 gtgccgacct tgccggcgta gcgaagctcg ccgccctcgt agaccccgac gagcagcgca
     3121 cccagaccgg tgcgcgagcc cttcggctcg gtgaacccgc cgaccaccac ctcctgctgg
     3181 tggacgcact tcagcttcag ccagtcctta ctacgccggg gctggtaggc cgacgcccgc
     3241 cgcttggcga tgagcccctc ccagccgagc ccgcaggcgg cggcgaggag tgcctcgccg
     3301 tcgccggtca gcacctcgcc caacgcgatc gggccacccg gaacgagcac ctcggcgagg
     3361 gcggcctggc gcgcctcgag cgggagcggt cggatgtcgt tgccgtcgaa ccacagcagg
     3421 tcgaacacca cgtactggac cggtaccgac gggtcgctgg ccttctgctg gaggtgctcg
     3481 aagctggtct ggccgtccac catggcgacc acctcgccgt cgaccagcag cgagccgcga
     3541 gccgcggcgg ccagggcccg ggcgacctcg gggtaggtgt cgtcgaacga gaggccgttg
     3601 cgggagcgca gccgcacgcc cccgtcgtcg acccacgcaa ggcagcggta gccgtcgagc
     3661 ttgcgctcga acacccagtc gtcgccgaac agccggcggt cggtcgagga ggcggccatg
     3721 accgagacct cggccggcgg gcggggcggg gccatcagac gatgaggccg aggcccttct
     3781 ggcagtgcgg tccgccccag tgctcgacga tcttgccgtc ggccatgcgc aagatggcgg
     3841 tgccgttgat gtggaccggt tcgccggtgg gaggcaggtc gaagaacggc tcgccgtcga
     3901 acgtgccgcg caccgaccag ttggagacga tgaggtcgtc cttctcgatg agcagatcca
     3961 ccgtgaacgc catgtccggg atcgcggcca tcaccgcccg ccaccagatc tgctcgtcgc
     4021 cccggacgtc ctcaccgacg cgctcgggcg caacccgctc ggtcacgtgg gtgtggaagt
     4081 ccggccccca gaagtcgaag acggcctcgg tgtccccggc gaacatcgcg ttggagtact
     4141 cgcccaggat cttcaggttc tcagacatgg ccggacgata ccgaccggcc cagcgccgtg
     4201 cccggaggag gcaacgggct tctgggtgcc gatgaccgtc agatcgcaac ggcagcgagc
     4261 cccacgaggg agcgcgggac cgccaggacg gatcgggcga cgagcccacg gcccgtccga
     4321 tctggtcggg gcgatccgct tcggcctggt cagcgtgccg gcgaaggcgt gcgccgcaga
     4381 agaggccggc tgagacgacg gcacccgaca agacggggtt cagcggagac cagccgacca
     4441 caccgccgag cgaacgggcg tgccccgggt cgtggcgatg cggccgacga catcgagcga
     4501 cgccgcgtga ccgatccgga cggcggctcc tccgagcggt tcgccacctt cacgcctcct
     4561 cgatgagcgc gccagcacct gctccctgtg gtgagcacgg cacggacgat ccgtcagcgg
     4621 tgcgctcgcc ggcccgtcgc gagcggtcag ccggccttcg ctcgtcttct gcgccgcccg
     4681 cggcgtcgcc gttgtgacgg gtgcccctgg ccctccgccg cccgtcgtgt taggcatgcc
     4741 taactgcatt ggagtcggag ggacccgcca tgcctgcact cgtcgaccgc ctggcctcgg
     4801 ccgccaccaa gccgctcacc cgcaccctca cggtcgaggc cgtgagcgac ctcgccgacg
     4861 ggttccggcg cgtcgacctc cgcagctcgg gcgtcctgcg ctgggagccg ggccagaagg
     4921 tgcaggtcca cgtgaagggg ttcgagtttc ggacctacac gccgttcgcc tgggatggcg
     4981 agcgggtgtc gctgctcgcg gcactcgacg ccaccggccc gggcaccgcg ctcgtcgcct
     5041 cgctcgaacc cggcaccgag gtctcggtgt tcgggccccg ccgggcgatg gacctgtcca
     5101 aggtcgcggc cgcgccgatc ctgatcggcg acgagacgtc gttcgccctc ggcgccgcct
     5161 gggaccagca cggctcggtg cccgccgccg cccacctcta cgaggtgacc cacccggagc
     5221 cctcggccca ggtgtgcgcg acgctcggct tcgaccacgc caccctcgtc gagcgcaccg
     5281 cggacgaggg ccatgccgac cggctgaccg agctcgctgt cgacctcgtc cgctccaccc
     5341 ccgacgcacc gctcgtcctc accggcaagg cccagaccat ccgtgccatc cgcggcgccc
     5401 tcaaggccgc cggcctgagc cccgccaccc gcgtgaaggc ccactgggac ccgaaccgct
     5461 ccggcctcga ctgaggccct cccccggcgg ctcgtccacc gacctccccc cacaagcgac
     5521 cgaacgttcg atgcggaccg ctgcggtgcg gcggctggcg tcgagacccg gcaaggccgg
     5581 ccctcggcgc gccgggtgcg tgcgaaggca ggaccggtcg tttcgtgcgc cgggcgaagg
     5641 gctccacccc gcaccgccga ggacccaggc atcttgctga ccgcgacgat ctcctgcgaa
     5701 gacccagccc gtcagcgtcc ggtcgctgcg gtcgccgcga tcagaagggc cgggtgtcgt
     5761 tcgtccaggt ggacccgtcc cagtagcgga gctgctggga ctgctgggga tcggggtgcc
     5821 atccgggagg tgtggcgcca tcgttcgagc ggacgagcgc cggcgccttc cggctggccg
     5881 accgggccac gaggatcgtg atcacgccgc acgcggccac ggcgccgagg aggaacaggc
     5941 ccatccccgg gctgccgtca tcgccaccgc tggtgctgaa cgcgccatcg tcgcccgtgg
     6001 gatcgtcgaa cgagaacggc gtgtcgccga aggggtcatc ggggtcgagc gcctggtcgc
     6061 gtccccacgc cggcgtgtag cgctcgtcgg gcacgacccg cttggggttg gcggcgaacg
     6121 cctccgtgtc gacgccatcg agcagcggct cgacctcggc ggggaccggc agcaccggca
     6181 cgtcggtcac ccaccagacc gcgtgctggg cggcgatggg gtcgggctcg gtcgcggcga
     6241 tggtggtgag gacggtggtc agcggctcct cggcaacgcc ggcccacgtc atggggacga
     6301 cctcggcggg gccggagtcc aggtgctggg cgcagaagcc ggccagctcg atctggtgct
     6361 ccccgggctc gatcgtgatg gtggggtcga ccccgggcct cacggtggcg gcgacctcat
     6421 cgatcgggcc gaccgtcacg gccgtctgct cgccctcgtc ctcggtggcg aacagcgccc
     6481 cgcggggtac ggcgagctgg acgggcgcat cggtgtggtt ctcgagggtg agcacgacgt
     6541 cgagcgtgtc gtcgctgccg aagcccccgg ctgccgatcc ggtgacggcc ttcgatgtac
     6601 cggccagcgc ctcgcggatg gacggcgggg ccgcggtggt gggctcggtc ggggtggtgg
     6661 actcggccgg cgcagccggg tcctcctggg caccagcccc gggaccggtc aacccgatga
     6721 gcagcgcgat cgctgctgct cccgccagac gcaccgcacc tcgaaccgcc atggtgacct
     6781 cctcaggccc gctctcgcgc caacgctacc tgggacccgc cgatcgcgcc gcaccgttcc
     6841 accccacccc accccacccc accccacccc acccgaacga acttggtgtg gaaaccgagg
     6901 gagagcatca gttcgcacac caagttcagg cgtctgcggg cgccgcggcc gccgcggcgg
     6961 ggacagaggc cggtgtccct cggttcgtcg gggagcacga acgttgttcc gacgcagaac
     7021 gaaccacgga gatcgtgcgc cgcatctgac cagacggcct tgccaaggcg acctca
//
LOCUS       DATMFS010000391         4935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_13710, whole genome shotgun sequence.
ACCESSION   DATMFS010000391 DATMFS010000000
VERSION     DATMFS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13710"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /locus_tag="VNQ33_07260"
     CDS             complement(<1..2250)
                     /locus_tag="VNQ33_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HWJ97942.1"
                     /translation="MAALGDLIPTPPDADAVLDAFTTWVEDRGLSMYPHQEEALLEIV
                     AGSHVIVDTPTGSGKSLIAVAAHFAALAARRRSYYTAPIKALVSEKFFDLSAAFGADN
                     VGMLTGDASVNPEAPIICCTAEILANLALREGAEADVGQVVMDEFHYFADPDRGWAWQ
                     VPLLELRDAQLILMSATLGDVTRFTETLPERTGRDVAVVRSATRPIPLVHQFELRPLT
                     EVLEELLATHQAPVYVVHFTQASAVERAQSLLSTNLLTRPEKDEIAELIGGFRFAPGF
                     GKVLAKFVRHGIGVHHAGMLPKYRRLVERLAQAGLLKVICGTDTLGVGINVPIRTVVF
                     TALAKYDGTSTRLLSAREFHQIGGRAGRAGFDTMGTVIALAPDHVIENHKAAMKAAAR
                     TEQDKKVRKIQKKKPPPGQVSWGQPTFDRLVGAPPEPLTSSFTVSHSMLLDVLDRPGN
                     GRAALHRLLTDNYETETRQAEHVARAEALFEALRDAEVVEELDEPDDWDRTVRVTIDL
                     QQAFALNQPLAPFALAALDLLDPESDTYVLDIVSVIEAVLDDPRQILSAQLSKAKGEA
                     VAAMKAEGMEYDERMAALDEITWPKPLADLLEPMFDVYRERHPWVAEHPLSPKSVARD
                     LWERAMDFGDYVRHYQLARSEGTVLRYLTDVYKTLLRTIPEDRKTDELADLTEWLGEL
                     VRQVDSSLLDEWEELRNPADPSDGSPADSDAIVERPPPALTSNRRAFTVLVRNAMFQR
                     VEHIGTRQWQ"
     gene            2561..3073
                     /locus_tag="VNQ33_07265"
     CDS             2561..3073
                     /locus_tag="VNQ33_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWJ97943.1"
                     /translation="MDISTRPVAPSPYDLLPAVPSFTVTSDDFADGARLDDEFVYSGG
                     NTSPQLSWSGAPEGTKSFAITCFDPDAPTGCGFWHWFTIGVSADTTSVARGAAAPGSH
                     CARNDFGNDGYDGCAPPEGDREHRYIFAIHALDTDDTGVPAGASPTYASFVHVGHTIA
                     RATITGTWSC"
     gene            3123..4208
                     /locus_tag="VNQ33_07270"
     CDS             3123..4208
                     /locus_tag="VNQ33_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97944.1"
                     /translation="MPPELELGRSNNNLDVVRHDGRLWLGLRTAPTHYASAEARLEVL
                     VSDDEGASWVRDRTIAMGRDVREPRFVSWQGRLLLYWFTAGTKGTRFQPDVIWVSERG
                     ADGAWDEPEAISPPDCVVWRVRPVDDQLCMTRYRGAGSLFTAHPVPLTVELWVSGDGR
                     RWVPADPVHPAVHDGGAEADFVQLPDGRVTAVVRKEGPEGGWGSDIVLAPHFDRTTWI
                     TRHDPRKFDSPYLFLDQGVPHLIARRQVAFRGRYDLGLSWLPDGVRTKANQLAYWLTP
                     KRTALYRIDPDALTATWLADLPSAGDTAFVGSVATADGQGHLVFNYSSPVERRWWPWF
                     VGQLCPTHVYSVELRPDPGGDGGSTSS"
     gene            complement(4151..>4935)
                     /locus_tag="VNQ33_07275"
     CDS             complement(4151..>4935)
                     /locus_tag="VNQ33_07275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWJ97945.1"
                     /translation="VLDLDRSLLDPALSALATDLGQDGGYWVVPVPDAGSDVPALITV
                     WSQPQVGRPDGHAYGVQMAHSYIELILRWSQHVQALRDAALRDPLTGLANRKALFDAL
                     EAGTTNGALLFCDLDRFKPVNDRYGHGAGDEVLRQIARRIERAVRSGDVVARTGGDEF
                     VVLAPSITLEQAAALAQRIRTAVAVPTALPAGTGTVSVGVTIGVAHADDALSEATLHG
                     ADQALVLAKANARGTVRWAPGPMPHDEVLPPSPPGSGRSSTE"
BASE COUNT          666 a         1713 c         1842 g          714 t
ORIGIN      
        1 ctgccactgg cgggtgccga tgtgctcgac ccgctggaac atcgcgttgc ggaccagcac
       61 ggtgaacgcc cgccggttcg acgtgagcgc gggtggcgga cgctcgacga tcgcatcgga
      121 gtccgcgggc gaaccgtcgg agggatcggc cgggttgcgg agctcctccc actcgtcgag
      181 gaggctggag tccacctggc ggaccagttc gcccagccac tcggtgaggt cggcgagctc
      241 gtcggtcttg cggtcttcgg ggatggtgcg cagcagcgtc ttgtagacgt cggtcaggta
      301 ccggagcacg gtgccttcgg agcgggcgag ctggtagtgg cggacgtagt cgccgaagtc
      361 catggcccgc tcccacaggt cgcgggcgac ggacttgggc gacagcgggt gctcggccac
      421 ccaggggtgg cgctcgcggt agacgtcgaa catcggctcg agcaggtcgg ccagtggctt
      481 gggccaggtg atctcgtcga gcgcggccat gcgctcgtcg tactccatgc cctcggcctt
      541 catggcggcc accgcctcgc ccttggcctt cgagagctgg gcgctcagga tctggcgggg
      601 gtcgtcgagc acggcctcga tcaccgagac gatgtcgagc acgtacgtgt cggactcggg
      661 atccagcagg tccagcgcgg ccagggcgaa gggggccagc ggctggttga gggcgaaggc
      721 ctgctggagg tcgatggtga cccgcacggt gcggtcccag tcgtcgggct cgtcgagctc
      781 ctccaccacc tcggcatcgc ggagcgcctc gaacagcgcc tcggcccggg cgacgtgttc
      841 ggcctggcgg gtctcggtct cgtagttgtc cgtgagcagg cggtggaggg cggcccgtcc
      901 gttgccgggg cggtcgagca cgtcgagcag catcgagtgg ctgacggtga agctcgaggt
      961 gagcggctcg ggcggcgcgc ccaccagccg gtcgaacgtc ggctggcccc acgacacctg
     1021 gccgggcggc ggcttcttct tctggatctt gcggaccttc ttgtcctgct cggtgcgggc
     1081 cgccgccttc atcgccgcct tgtggttctc gatgacgtgg tcgggggcga gggcgatgac
     1141 cgtgcccatc gtgtcgaagc cggcgcgccc ggcccgcccg ccgatctggt ggaactcgcg
     1201 tgcgctgagc agccgggtcg aggtcccgtc gtacttggcc agggcggtga acacgaccgt
     1261 gcggatgggc acgttgatgc cgacgcccag cgtgtcggtg ccgcagatga ccttgagcag
     1321 cccggcctgg gcgagccgct ccaccaggcg ccggtacttg gggagcatgc cggcgtggtg
     1381 gacgccgatg ccgtggcgca cgaacttggc gagcaccttg ccgaaccccg gagcgaaccg
     1441 gaacccgccg atcagctcgg cgatctcgtc cttctcgggt cgggtcagca ggttggtgct
     1501 cagcagcgac tgggcccgtt cgacggcgga cgcctgggtg aagtggacga cgtagacggg
     1561 cgcctggtgc gtggcgagca gctcctcgag cacctcggtg agcgggcgca gctcgaactg
     1621 gtggacgagc gggatcggcc gggtggccga ccgcacgacg gccacgtcgc gcccggtgcg
     1681 ctcgggcagg gtctcggtga agcgggtgac gtcgccgagc gtggccgaca tcaagatgag
     1741 ctgggcgtcg cgcagttcga ggagcgggac ctgccaggcc cacccgcggt cggggtcggc
     1801 gaagtagtgg aactcgtcca tcaccacctg gccgacgtcg gcctcggccc cctcgcgcag
     1861 cgccaggttg gcgaggatct ccgcggtgca gcagatgatc ggcgcctccg ggttgaccga
     1921 tgcgtcgccg gtgagcatgc ccacgttgtc ggcgccgaac gcggccgaca ggtcgaagaa
     1981 cttctccgac acgagcgcct tgatcggcgc cgtgtagtag ctgcggcggc gcgccgcgag
     2041 cgcggcgaag tgggcggcca cggcgatgag gctcttgccc gatccggtgg gcgtgtcgac
     2101 gatgacgtgc gatccggcga cgatctcgag gagggcctcc tcctggtgcg ggtacatgga
     2161 caggccccgg tcctcgaccc aggtcgtgaa cgcgtcgagg acggcatcgg cgtcgggtgg
     2221 cgtcgggatg aggtcaccga gcgcggccat ggccgcagcg tacggggtga tctccgaccg
     2281 ggaggatgcc gccatcgagc ggacgtcggc gaagaccact agtttgcgga ggcacgcagg
     2341 ccaccgaggg gagcggtcct gcgacggggg cagaggggga ggacatgagc gggttgcggc
     2401 tccacggtcg cagggaaccg gcagcgccgg tcgcgagcgc gcccccgacg atgcgcctcg
     2461 aggccccccg gccagcccgg gcccacccat cccgcgggtg cggcccggct ggttcgggta
     2521 caacacgggg ccaccaacca tccgtccccg gagacctgcc atggacatct cgacccgacc
     2581 cgtcgcgccc agcccctacg acctgctccc ggcggtgccg tcgttcaccg tcaccagcga
     2641 cgacttcgcc gacggcgccc gcctcgacga cgagttcgtc tactcggggg gcaacaccag
     2701 cccgcagctg agctggtcgg gcgcgcccga gggcaccaag tcgttcgcca tcacctgctt
     2761 cgaccccgat gcgccgaccg gctgcgggtt ctggcactgg ttcacgatcg gcgtctcggc
     2821 cgacacgacg tcggtcgccc gcggcgcggc ggcgcctggt tcgcactgtg cccgcaacga
     2881 cttcggcaac gacggctacg acggctgcgc gccgcccgag ggcgaccgcg agcatcggta
     2941 catcttcgcc atccacgccc tcgacaccga tgacaccggt gtgccggcgg gcgccagccc
     3001 gacctatgcc agcttcgtcc acgtcggcca caccatcgcc cgggccacca tcaccggcac
     3061 ctggtcgtgc tgaccgccgg gtgaggatct cggccccgcg cccggtcgcc cccgggccgg
     3121 ggatgccgcc ggagctcgag ctggggcggt cgaacaacaa cctcgacgtg gtgcgccacg
     3181 acggtcggct ctggctcggc ctgcgcaccg cgcccaccca ctacgcctcg gctgaggctc
     3241 gcctcgaggt gctggtgtcc gacgacgagg gcgcctcgtg ggtgcgggac cggacgatcg
     3301 cgatggggcg cgacgtgcgg gagccgcgct tcgtcagctg gcagggccgc ctgctcctct
     3361 actggttcac cgcggggacg aagggcacga ggttccagcc cgacgtcatc tgggtcagcg
     3421 agcggggcgc cgacggcgct tgggacgaac cggaggcgat ctcgccgccg gactgcgtcg
     3481 tgtggcgcgt ccgtcccgtg gacgaccagc tgtgcatgac gcggtaccgc ggcgccggct
     3541 cgctgttcac ggcgcacccg gtgccgctca ccgtcgagct gtgggtgagc ggcgacggcc
     3601 gtcggtgggt gccggccgac ccggtccacc ccgcggtcca cgacggtggg gccgaggccg
     3661 acttcgtcca gctgcccgat ggccgggtca ccgccgtggt gcgcaaggag ggacccgagg
     3721 gcgggtgggg cagcgacatc gtcctcgccc cgcacttcga ccggaccacg tggatcaccc
     3781 gccacgaccc gcggaagttc gactcgccgt acctcttctt ggaccagggc gtcccgcacc
     3841 tcatcgcccg gcgccaggtg gcgttccgcg gccgctacga cctcggcctg tcgtggctgc
     3901 ccgacggtgt ccgcacgaag gcgaaccagc tcgcctactg gctgacgccc aagcgcacgg
     3961 cgctgtaccg gatcgacccc gacgcgctca cggccacgtg gctggccgac ctgccatccg
     4021 ccggcgacac cgccttcgtc ggatcggtgg ccaccgccga cggacagggc cacctcgtct
     4081 tcaactactc gtcgccggtc gagcgccgct ggtggccgtg gttcgtgggg cagctgtgcc
     4141 cgacccacgt ctactcggtg gaactgcgcc cggatcccgg gggcgacggc ggcagcacct
     4201 cgtcgtgagg catcggccca ggggcccagc ggaccgtgcc gcgggcgttg gccttggcga
     4261 gcacgagcgc ctgatcggcg ccgtgcaggg tggcctcgct cagtgcatcg tcggcgtggg
     4321 cgacaccgat ggtcaccccg accgacaccg tgcccgtgcc tgccggcaag gcggtgggca
     4381 cggccaccgc ggtccggatg cgctgggcca gggccgccgc ctgctcgagg gtgatgctcg
     4441 gggcgagcac cacgaactcg tcgcccccgg tacgggccac cacgtctccg gaccggacgg
     4501 cccgttcgat gcggcgggcg atctggcgga ggacctcgtc gccggcgccg tggccgtacc
     4561 ggtcgttcac cggcttgaag cgatcgaggt cgcagaacaa gagcgcgccg ttggtggtcc
     4621 cggcctcgag cgcgtcgaac agggccttgc ggttggccaa cccggtgagc ggatcgcgca
     4681 gagcggcgtc gcgcagggcc tggacgtgct ggctccaccg caggatcagc tcgatgtagg
     4741 agtgcgccat ctgcaccccg taggcgtgcc cgtcgggccg gccgacctgg ggctgggacc
     4801 acacggtgat gagcgcgggc acgtcggagc cggcatcggg cacgggcacc acccagtagc
     4861 cgccgtcttg gccgaggtcg gtggccagcg cgctgagcgc cgggtcgagg agcgaccggt
     4921 cgaggtcgag caccg
//
LOCUS       DATMFS010000392         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_199774, whole genome shotgun sequence.
ACCESSION   DATMFS010000392 DATMFS010000000
VERSION     DATMFS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199774"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..796)
                     /locus_tag="VNQ33_07280"
     CDS             complement(155..796)
                     /locus_tag="VNQ33_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97946.1"
                     /translation="MTTTPQHPDRSAPRRLRRLAVVAAAVAILVTACMPNDFVKHKAG
                     SYWVWFGPKNYLESHSTYGIDIYAPTGPDTVAHTFDTTICASGSTLQASVDRYFPARR
                     AQIRDTSGYTNWVTTSATTPKRLAAGTYGPNYFRQEITFTGRTGGVAVRGEATLDYQL
                     PDTTYCFERLKFRATAANTFSTQMPRLRAIQENIIYSGPGACDPEQPDDPCPG"
     gene            complement(976..2619)
                     /locus_tag="VNQ33_07285"
     CDS             complement(976..2619)
                     /locus_tag="VNQ33_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HWJ97947.1"
                     /translation="MRRPRLRLVLGIALPLLVVVLLLAAWAIDTSSASGKVPRNTTLA
                     GRDVSTFGEDDLAATVADVADEYATTQVQVRTDEKSYKVPASKLGLMLDEDATVNAAL
                     DLDTGPIAWGTSFLEKRTVPLTFVVDHDQLKAGLAELGGNAKPTEPKIAAGADRFTVV
                     SGSDGTTINADGVAEQLRRRAESGELPLVVDAEAVAEAPKVSDASAQVLADQLTKGTA
                     NGLKVTSGTSTVDVPAPTARAWLSSKVDGDQLVAQLDAEKAKADLTSLLPDATEPKDA
                     QIVLANGAPQILPSSDGATCCAADTPVRLLAAINGGSGAVEIGLDVKKAAFTTEAAEK
                     LGIKEPVGTTTEWKGVQQVKSFTTYYPCCAARVTNIQRIADIVSGTVVKPGEKFDMNA
                     IVGQRTTDKGFVSAGAIANGEHVDEVGGGVSQFATTTFNAAFFAGLPINTYQAHSEHF
                     DRYPYGREATMGYPNPNLVWTNNTPYGILVEATHTDTSVTVRLWSTQYAYGEQTGQTT
                     GKSGSCTTVATTRTIHYPDGRTGTDTFQARYRVPGQTSC"
     gene            2786..>3515
                     /locus_tag="VNQ33_07290"
     CDS             2786..>3515
                     /locus_tag="VNQ33_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ97948.1"
                     /translation="MTGAGRGIGREHSLMLADQGAKVVVNDIGGEMDGSGRSTGPAQE
                     VVDEIVAGGGEAVVNGDDISTWDGAERLVQQAIDHFGRLDVLVSNAGILRDRMLANMT
                     EAEWDAVIAVHLKGTFGPARHAAAYWREQTKAGEAVDGRIINTSSPSGIYGNVGQTNY
                     GAAKAGIAAFTVIAAKELARYGVTVNAIAPAALTRMTENLGMGQADDLTKAMLSPRWI
                     APIVTWLASSESRDVTGRVFDVTGQ"
BASE COUNT          462 a         1122 c         1369 g          562 t
ORIGIN      
        1 atccgtgaac cgcagcgcat cacccatggg ccagacctcc gagcagcacc agcggccggt
       61 gccagccgcc acagtctgcc aggggtcgcg aggcccgggg cccggtcacc tcgctcgatg
      121 gtggtcgagc ccggtgaccg gcccggtaac cggttcagcc cgggcagggg tcgtcgggct
      181 gttcggggtc gcaggcgccg ggccccgagt agatgatgtt ctcctggatg gcccggaggc
      241 ggggcatctg ggtgctgaac gtgttcgctg ccgtggcccg gaacttcaac cgctcgaagc
      301 agtaggtggt gtcggggagc tggtagtcga gcgtggcctc gccgcgcacg gccaccccgc
      361 cggtccgtcc cgtgaacgtg atctcctggc ggaagtagtt gggaccgtag gtgccggccg
      421 caagccgctt cggcgtcgtg gccgacgtgg tgacccagtt ggtgtagccc gacgtgtcac
      481 ggatctgtgc ccggcgggcc gggaagtacc ggtcgaccga tgcctgcagg gtgctgccgc
      541 tggcgcagat cgtcgtgtcg aacgtgtggg cgacggtgtc ggggccggtg ggcgcgtaga
      601 tgtcgatccc gtaggtcgag tggctctcca ggtagttctt gggcccgaac cagacccagt
      661 agctcccggc cttgtgcttc acgaagtcgt tcggcatgca ggcggtcacg aggatcgcga
      721 cggcggcggc gacgacggcc agccggcgca gtcgccgggg cgcggatcga tcggggtgct
      781 ggggtgtcgt ggtcacggtc gctccagggt tcgaggcggg gaggggtcga ggagctgatc
      841 gtcgctcgcg gcgcgcgaac aaggcgcgaa cggcccgatg gccgcggcgc gaacggaggc
      901 agcgagccgg ggcgccgcct gtcggtcccg gcggccggca cgggccccgg gtcgttcccg
      961 cgcggcgagg ccccgtcagc agctggtctg gcccgggacc cggtagcggg cctggaaggt
     1021 gtcggtgccg gtcctgccgt ccgggtagtg gatggtgcgg gtggtcgcga cggtggtgca
     1081 cgacccgctc ttgccggtgg tctggccggt ctgctcgccg taggcgtact gcgtggacca
     1141 gagccgcacc gtcacgctgg tgtcggtgtg ggtggcttcg acgaggatcc cgtagggcgt
     1201 gttgttggtc cacacgaggt tcgggttcgg gtagcccatg gtggcctcgc ggccgtaggg
     1261 gtaccggtcg aagtgctccg agtgggcctg gtacgtgttg atcggcagcc cggcgaagaa
     1321 ggcggcgttg aaggtcgtgg tggcgaactg cgagacaccg ccgccgacct cgtcgacgtg
     1381 ctcgccgttg gcgatggctc cggcggacac gaagcccttg tcggtggtgc gctggccgac
     1441 gatggcgttc atgtcgaact tctcgcccgg cttgacgacc gtgccgctga cgatgtcggc
     1501 gatgcgctgg atgttggtga cgcgggcggc gcagcagggg tagtaggtcg tgaagctctt
     1561 gacctgttgg acgcccttcc actcggtcgt ggtgccgacg ggctctttga tgccgagctt
     1621 ctcggcggct tcggtggtga acgccgcctt cttgacgtcg aggccgatct ccacggcacc
     1681 cgagccgccg ttgatggcgg cgaggagccg gaccggcgtg tcggcggcgc agcacgtggc
     1741 gccgtcggag ctcgggagga tctgcggcgc gccgtttgcg aggacgatct gggcgtcctt
     1801 cggctcggtg gcatcgggaa gcaggctcgt gaggtcggcc ttggccttct cggcgtcgag
     1861 ctgggcgacg agctggtcgc cgtcgacctt cgaggagagc cacgcccggg cggtgggggc
     1921 ggggacgtcg accgtgctgg tgccgctggt gaccttgagg ccgttggccg tgcccttggt
     1981 gagctggtcg gcgaggacct gggcggacgc gtcggacacc ttcggggcct cggcgacggc
     2041 ttcggcatcg accaccagcg gcagttcgcc gctctcggcg cggcggcgga gctgctcggc
     2101 gaccccgtcg gcgttgatgg tggtgccgtc ggacccgctg accacggtga accggtcggc
     2161 tccggcggcg atcttgggct cggtgggctt ggcgttgccg cccagctcgg ccagcccggc
     2221 cttgagctgg tcgtggtcga ccacgaaggt cagcgggacg gtgcgcttct cgaggaacga
     2281 cgtgccccag gcgatgggcc cggtgtcgag gtcgagcgcc gcgttcacgg tggcgtcctc
     2341 gtcgagcatg aggcccagct tggaggcggg gaccttgtag ctcttctcgt cggtccggac
     2401 ctgcacctgg gtggtcgcgt actcgtcggc cacgtcggcg acggtggcgg ccaggtcgtc
     2461 ctcgccgaag gtcgacacat cccgcccggc gagcgtggtg ttgcggggga ccttgccgct
     2521 ggcgctcgac gtgtcgatgg cccaggccgc cagcaggagc acgacgacca ggaggggcag
     2581 ggcgatgccg aggacgagcc tgaggcgggg acgacgcatc cgaccacctt ggcacgcgga
     2641 tgcacccgtt ccgtggcagt ggaccgtgcc ggacggcgca ccagcgggct gtcctgcagg
     2701 tttgccaaga tgggcgctca tcgagtccgc gcgagcgcgg ggcaagggga tggccgtggg
     2761 gatctgcgac ggacgtgtgt gcatcgtgac cggtgccggc cgcggcatcg gacgggagca
     2821 ctcgctgatg ctcgccgacc agggcgccaa ggtcgtggtc aacgacatcg gcggcgagat
     2881 ggacggcagc ggccggtcca ccggcccggc ccaggaggtg gtcgacgaga tcgtcgccgg
     2941 tggcggcgag gccgtggtca acggtgacga catctccacc tgggacggcg ccgagcgcct
     3001 cgtccagcag gccatcgacc acttcggccg actcgacgtg ctcgtcagca acgccgggat
     3061 cctccgggac cggatgctcg ccaacatgac cgaggcggag tgggacgccg tcatcgccgt
     3121 ccacttgaag ggcacgttcg gcccggcccg ccacgccgcg gcgtactggc gtgagcagac
     3181 caaggcgggc gaggcggtcg acggccgcat catcaacacg tcgtcgccgt cgggcatcta
     3241 cggcaacgtc ggccagacga actacggcgc cgccaaggct ggcatcgccg cgttcacggt
     3301 catcgccgcc aaggagctcg cccgctacgg cgtcaccgtg aacgccatcg ccccggccgc
     3361 gctgacgcgc atgaccgaga acctgggcat gggccaggcc gacgacctga ccaaagccat
     3421 gctctcgccg cgctggatcg caccgatcgt gacctggctg gcctcgtcgg agtcccgcga
     3481 cgtcacgggc cgggtgttcg acgtgaccgg ccagg
//
LOCUS       DATMFS010000393         4860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_267370, whole genome shotgun sequence.
ACCESSION   DATMFS010000393 DATMFS010000000
VERSION     DATMFS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267370"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1234)
                     /locus_tag="VNQ33_07295"
     CDS             complement(113..1234)
                     /locus_tag="VNQ33_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWJ97949.1"
                     /translation="MRRIPRSTAFGLTAVAVAASLVLAACSGSSDAGPSGSPDSTSSG
                     DGGGKATTTTVVAAAPAGERANGPAVPSAGCGESVQQPVTKEKQFLDDSDRWFLLTTP
                     DELDQDTPLPMVLDFHGLAEGADVHAQMTLLPEYGQEHGFLVVSPHGTGSPVRWAVDP
                     DVEANEDLQYTQDLLDQVEAEQCVDLSRIYATGLSNGAFMSSVLACTMADRFAAVAPV
                     AGLIRPESCDPSRPVPVLAFHGTADPILLFNGGVGDRLNNVLGGGGAADSADEKPVPE
                     ADLDGPGYPAAAQEWATGNGCTGKPIDDDLSDTVIERTWDCPADAVVEFLIVEGGGHT
                     WPGSEFSNQLADIMGPTDMATNANDLIWDFFQRFQLPAA"
     gene            1465..3879
                     /locus_tag="VNQ33_07300"
     CDS             1465..3879
                     /locus_tag="VNQ33_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWJ97950.1"
                     /translation="MSRPAAAAALTAAEVVAKLDRDAKVRLLSGADRFALEAVPGFGI
                     EGVDIADGPHGLRHQQRGGDHLGLQGSVPATCFPTAAALGSSWDVELLEAVGVALGEE
                     AAEQGVAVVLGPGINIKRHPAGGRCFEYLSEDPVLSGKLAAAMIRGVQSQGIGTSLKH
                     FAVNNHESHRFVVDAVVDERTLRELYLTGFEIAVRESAPWTVMCSYNLVNGTYASEHR
                     HLLQEVLRDAWGFEGLVMTDWGAANDRVAGIKAGLDLEMPGSAGAFDADVLAALDSGE
                     LDEADVDRCAERVVALLQRARRDRPASDHDAHHELGRRAAAAGAVLLANDGVLPLSAS
                     GLDRIAIVGSFAETPRFQGAGSSQVNPTKVDRLLDVVRDRVGSDAVVDYAAAFDSSSG
                     ATTDALIDEALAVAAEADVVVFCGGLPAPMESEGFDRTTLDLPAGQIAVIEALAALSV
                     PVVVVLSNGGVVHLPFSDRVAAVLECWLGGQAGAGGIVDVLFGDADPGGRLAESIPEH
                     VAQLPSDRNFPGQPRQAQYREGPYVGYRFHDTAGVPARFAFGHGLSYTRFAWDDIALS
                     GTGTDLTVSVTVANTGDRAGSDVVQVYVHDRECSVHRPAKELKGFAKVHLLPGESETV
                     SIVLDRRAFAIWDVAAQDWLVEAGTFDIAVARSSVEAVTFLPVTVESDDVVTAVPKPA
                     GMVATDDEFAVLLGKPIPVVPPARPFHRNSTLEDLESSPIGRLLGAAVVREGLRQSLS
                     EFPDPDEATLTMVRTALREGPVRALVLLSDGLVRFAAVDAILATLNGNWSEAGGLVKK
                     ALRPKR"
     gene            complement(3940..4013)
                     /locus_tag="VNQ33_07305"
     tRNA            complement(3940..4013)
                     /locus_tag="VNQ33_07305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3977..3979),aa:Arg,seq:cct)
BASE COUNT          655 a         1634 c         1832 g          739 t
ORIGIN      
        1 gtgtgaaatc tgacgttggg cagcaggaac cacaccaact tcgagtggat cccgccgatc
       61 tcggcgcacg accgccagca ggccagcccg tggcgggtca ggccgcggcg ggtcaggccg
      121 cggggagctg gaagcgctgg aagaagtccc agatgaggtc gttggcgttc gtggccatgt
      181 cggtgggccc catgatgtcg gcgagctggt tgctgaactc gctgccgggc caggtgtggc
      241 cgccgccttc gacgatcagg aactcgacga ccgcgtcggc agggcagtcc caggtgcgct
      301 cgatgaccgt gtcggagagg tcgtcgtcga tgggcttgcc ggtgcacccg ttgcccgtgg
      361 cccactcctg ggcggcggcc gggtagccgg gtccgtccag gtcggcctcg ggaacgggct
      421 tctcgtcggc ggagtcggcg gcaccgcctc cgccgaggac gttgttgagc cggtcgccga
      481 cgccgccgtt gaacagcagg atcgggtcag cggtcccgtg gaaggcgagg acggggacgg
      541 ggcgcgacgg gtcgcacgac tcggggcgga tcagcccggc gacgggagcg acggcggcga
      601 agcggtccgc catggtgcac gccaggaccg acgacatgaa cgcgccgttc gacaggccgg
      661 tggcgtagat gcgggagagg tcgacgcact gctcggcctc cacctggtcg agcaggtcct
      721 gcgtgtactg gaggtcctcg ttggcctcga cgtcggggtc gaccgcccac ctcaccggcg
      781 agccggtccc gtgcgggctg acgacgagga acccgtgctc ttggccgtac tcggggagga
      841 gggtcatctg cgcgtggacg tcggcgcctt cggcgagacc gtggaagtcg agcaccatcg
      901 gcagcggcgt gtcctggtcg agctcgtcgg gggtggtgag caggaaccag cggtcgctgt
      961 cgtcgaggaa ctgcttctcc ttggtgaccg gctgctggac cgactcgccg catccggcgg
     1021 acgggacggc agggccgttg gcccgctcgc cggcaggcgc cgcggcgacg accgtcgtgg
     1081 tggtggcctt accgccgccg tcgcccgagg aggtgctgtc ggggctcccg gacggaccgg
     1141 cgtcggagct gcccgagcag gcggccagca ccagcgacgc ggcgacggcg acggcggtca
     1201 gcccgaaggc ggtggagcgg gggatgcggc gcatggtgcc tcctgggtcg ccggccggcg
     1261 acagagcgga ccgatcgtat tcgttggtcg ttggcgtcgg cgtcggcgtc ggcggtgacc
     1321 gtgacggtga cgtgtgccgt ggacgtcgag cggtggcggg tcgtcgtcgt gtcccggttg
     1381 ggcggtccgg tcggggcgtc ggcggcgtcg acgaacgcgg gcctccccgg gaagcggacc
     1441 ggcgggttcc cctacgatcc cggaatgagc cgtcccgccg ccgctgcggc cttgaccgcc
     1501 gccgaggtcg tggccaagct cgaccgcgac gcgaaggtgc ggctgctgtc gggcgccgac
     1561 cggttcgcgc tggaggcggt cccggggttc gggatcgagg gcgtcgacat cgccgacgga
     1621 ccgcacggcc tccgccacca gcagcggggc ggcgaccacc tcggtctcca gggctcggtt
     1681 ccggccacgt gcttcccgac cgctgcggca ctcggctcgt cgtgggacgt cgagctcctc
     1741 gaggcggtcg gcgtggcgct gggggaggag gcggccgagc agggcgtcgc cgtcgtcttg
     1801 ggcccgggca tcaacatcaa gcgccacccc gccgggggcc ggtgcttcga gtacctctcc
     1861 gaggatccgg tgctgagcgg gaagctggcc gcggccatga tccgcggcgt ccagtcccag
     1921 ggcatcggga ccagcctcaa gcacttcgcc gtcaacaacc acgagtccca ccggttcgtg
     1981 gtcgatgccg tcgtcgacga gcgcacgctg cgggagctgt acctgaccgg cttcgagatc
     2041 gccgtgcgcg agtctgctcc gtggacggtc atgtgctcct acaacctggt gaacggcacg
     2101 tacgcgtctg agcaccgcca cctgctgcag gaggtgctgc gcgacgcgtg gggcttcgag
     2161 ggcctggtga tgaccgactg gggcgcggcc aacgaccgcg tcgccggcat caaggccggc
     2221 ctcgacctgg agatgccggg cagcgcgggg gccttcgacg ccgacgtgct cgccgccctc
     2281 gactcgggcg agctcgatga ggcggacgtc gaccgctgcg ccgaacgggt cgtcgccctg
     2341 ctccagcggg cccgacgtga ccgcccggcc agcgatcacg acgcccacca cgagctcggc
     2401 cgccgggccg cggcggccgg ggcggtgctg ctggcgaacg acggcgtgct accgctctcg
     2461 gcctccggtc tggatcggat cgcgatcgtc ggctcgttcg ccgagacgcc ccggttccag
     2521 ggtgcgggca gctcgcaggt gaacccgacc aaggtggacc gcctgctcga cgtcgtgcgg
     2581 gaccgggtcg ggtcggacgc ggtggtcgac tacgccgccg cgttcgactc ctcgtcgggt
     2641 gccaccaccg acgcactcat cgacgaggcg ctcgccgtcg cggccgaggc cgatgtcgtc
     2701 gtgttctgcg gcgggctgcc ggcgccgatg gagtccgagg gcttcgatcg caccaccctc
     2761 gacctccccg ctggccagat cgccgtgatc gaggcgctcg ccgcgctgtc ggtcccggtg
     2821 gtggtcgtgc tcagcaacgg cggcgtcgtc cacctgccgt tctcggaccg ggtggcggcg
     2881 gtgctcgagt gctggctcgg cggccaggcc ggcgctggcg gcatcgtcga cgtcttgttc
     2941 ggcgacgccg atcccggcgg ccgcctcgcc gagagcatcc ccgagcacgt ggcccagctc
     3001 ccgtcggacc gcaacttccc cggccagccc cgccaggccc agtaccgaga agggccctac
     3061 gtcgggtacc gcttccacga cacggccggc gtcccggctc gcttcgcctt cgggcacggg
     3121 ctctcgtaca cccgcttcgc ctgggacgac atcgccctgt cgggtacggg caccgacctg
     3181 accgtctccg tcaccgtcgc caacaccggc gaccgggcgg gcagcgacgt cgtgcaggtg
     3241 tacgtccacg accgcgagtg ctccgtgcac cgcccggcga aggagctgaa gggcttcgcc
     3301 aaggtccacc tgctgcccgg ggaatcggag acggtgtcga tcgtcttgga ccggcgggcc
     3361 ttcgccatct gggacgtggc cgcccaggac tggctcgtcg aggccggcac cttcgacatc
     3421 gccgtcgccc gctcgtcggt cgaagccgtc acgttcctgc cggtgaccgt cgagtccgac
     3481 gacgtcgtga ccgccgtgcc gaagccggcg ggcatggtcg ccaccgacga cgagttcgcc
     3541 gtcctcctgg gcaagccgat cccggtggtc cctccggcgc ggccgttcca ccggaactcc
     3601 acgctcgagg acctggagtc ctcgcccatc ggccgcctcc tcggcgccgc ggtggtgcgg
     3661 gaggggctgc ggcagtccct gtccgagttc cccgaccccg acgaggccac cctcacgatg
     3721 gtccgcaccg ccctcaggga aggcccggtc cgggccctcg tcttgttgag cgacggcctg
     3781 gtcaggttcg ccgccgtcga cgccatcctc gccaccctca acggcaactg gtccgaagcg
     3841 gggggcctgg tgaagaaggc cttgcgcccc aagcgctgac ggctcggcgc cgcccgttcc
     3901 ggtgcggccg ccgaccgaaa gtggagcgac atcacagagc gcccccgaca ggaatcgaac
     3961 ctgtggccta ccgcttagga ggcggttgct ctatccaact gagctacgag ggcggggccg
     4021 gaagagaacg cgggagaaac ctcctacggc ctcagatccg actgagcgaa cgatcctacg
     4081 acggtcgttc gagccggccg cgagccgttg cagccgtcca acgccgcctt ccgtatgttt
     4141 cgcctcgcgt ccgggcgtgg cgaagccact cgagccgccg atcggaggtc gggtcaggct
     4201 gcgagcagaa gggggtaggt cgccgtggcg ggcctggtgg cgccagaggg caaggcagtt
     4261 gacggtgagc gcggtaggtc aacagattga ggtgctgtcg aacgtgcccg accgagtggg
     4321 ctcggcggag ccagagtgtg tcgtcgaggg cccggttgat ggactcggcg tcgtttgcgt
     4381 cggcggtaga gccgtttgaa gtcgggtcgg tctgggggtc tggtggatgt tctcggttcg
     4441 gttgaaccgc cgcttcgtgt ccgcgccgag acggcacggc tcctaccgtg gccggtgtcg
     4501 gcacctctcc aaaggttccg tccagccccg gggagtgttc gcgcaccgcc gggaccactt
     4561 gttgttcagg agcggaccag accgagaaat tccaatggtg tcgcaggcgg aggggtccga
     4621 acccgtagac actgcggtct tcgtctcatt ccttcgaaca gtgttgtacg gttactgcgt
     4681 tctagtggga gggaggcccg tgatcgaagg cgacctggac gatgcgggtg gcggtgagcc
     4741 gatcgcagac ggtgggggta gtggcggagg cggaggcggt gacccaaagc cgccgaagaa
     4801 ggtgcccgaa gatcgacgca tgaggcttct gcgcgagggg ggcgggggcg ggggcggggg
//
LOCUS       DATMFS010000394         5125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_267449, whole genome shotgun sequence.
ACCESSION   DATMFS010000394 DATMFS010000000
VERSION     DATMFS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267449"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1147)
                     /locus_tag="VNQ33_07310"
     CDS             complement(101..1147)
                     /locus_tag="VNQ33_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ97951.1"
                     /translation="MEPVDDWVDAVTHATSPPELSTVGPDFAVVHEGLEVRRHDGLTP
                     GTDQELDGFAFRTLPAPGELLTTFATVNDVHFGEEVCGLIDGSDVGPVFRTEPGEDAF
                     PEIMNRGAIAEMTAIDPAAVLVKGDLTSNGTVEEYEAFRAAYEPVFGDRLTVVRGNHE
                     SYHRAPFAAEPFQQVDLPGVSLVVIDTSIDGGVPGQVTPDQLEQLDEVAARADRPILL
                     FGHHHLGDPDSPEKADRTFGIGLEDSAALLELVARRPAIKAYSAGHTHRNRVRRFGPT
                     GDVPWVEVACVKDYPGTWAEYRVHEGGIVQIAHRISTPEALAWTEMTRHMYHGLYHDY
                     ALGQLADRCFAIST"
     gene            1109..1618
                     /locus_tag="VNQ33_07315"
     CDS             1109..1618
                     /locus_tag="VNQ33_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ97952.1"
                     /translation="MGDRIHPVVDRFHAADPTGAPARQPGAGDTCDPSSILSDRYGSF
                     LRPLRTSRGMTQAQLAETTGISQPNLSAYENSRREPTLDVLNRIVVACGYQLVAEAGA
                     KQIPVPLPRVGWFPLEDLPPRLPDDPPDEPSTMPWDTPTNVRIAAIHARQAGAGSSGE
                     RHRPRRRDA"
     gene            1967..2149
                     /locus_tag="VNQ33_07320"
     CDS             1967..2149
                     /locus_tag="VNQ33_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97953.1"
                     /translation="MFKLSFNRPKDWVDVQRVIRNGPDLDTDSIERVLVEMRGPTRHP
                     RVARLRAMIRSGGDLG"
     gene            complement(2156..2338)
                     /locus_tag="VNQ33_07325"
     CDS             complement(2156..2338)
                     /locus_tag="VNQ33_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HWJ97954.1"
                     /translation="MTQVQISGDPTEEEVAAIVAAVDAATPRGAAPVPSTAPPRWRWS
                     GRWWSRPIPQRRARPW"
     gene            complement(2335..3885)
                     /locus_tag="VNQ33_07330"
     CDS             complement(2335..3885)
                     /locus_tag="VNQ33_07330"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWJ97955.1"
                     /translation="MPEHPIRERLDDLLARKEQARTPGSERSIQRQHDRGKMLARERI
                     EYLLDEGSFRELDMLARHRSPDVKDRPYTDGVITGWGTVEGRTVFVFSQDFTLFGGAL
                     GEVFAEKIHKVMDMALKVGAPVVGINDGAGARIQEGPVSLASYGGIFHRNVMASGVTP
                     QISVIMGPCAGGAVYSPALTDFIFMVEDTSYMFITGPDVVKTVTGEDVTQQELGGAQT
                     HASKSGIAAFTAPDDKAVLDDVRFLLSYLPLNNLETAPVIDTGDPIDRETPELLDLIP
                     ASANVPYDMRKVVEAVADDNEFFEYFSKWATNIVCGFVRLGGQSVGVVGNQPAAYAGV
                     LDIEASEKAARFVRTCDSFNIPLVTFVDVPGFLPGVDQEYNGIIRHGAKLLYAYCEAT
                     VPRIQVITRKAYGGAYVVMDSKSIGADLAYAWPSAELAVMGATGAVEILNRKEIAEAD
                     DKDAKKAELIDAYSEKWLNPYVAAERGYVDDVIDPSVTRQMLIDGLRMLESKKEELPP
                     RKHGNMPL"
     gene            4139..4690
                     /locus_tag="VNQ33_07335"
     CDS             4139..4690
                     /locus_tag="VNQ33_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ97956.1"
                     /translation="MPDTPRISPLPPDGRDPRTQALLQSLKFDPEGEDMNLFATLAHH
                     PRLLRRWSQFGGLLLAGGTLSPRDREVLILRTAANCGAGYEWGHHLPIGEAAGLTTNE
                     MTALAGSRREAEGVDQDLIRAADELHDRNVIIPETWAALAARFDEQQLIEICMVVGQY
                     HLVAFTLRSLGIQREAGVEGLPE"
     gene            4687..4848
                     /locus_tag="VNQ33_07340"
     CDS             4687..4848
                     /locus_tag="VNQ33_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97957.1"
                     /translation="MSRLEGRKILEVGAGTRKVDDPDTPAGNGRAIAVTAASEGATVA
                     CADIDLAAA"
     gene            4864..4998
                     /locus_tag="VNQ33_07345"
     CDS             4864..4998
                     /locus_tag="VNQ33_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97958.1"
                     /translation="MRDVGGHAQVIVADVSDAAACTAMVAKAADLLEGLDGVVVNATL
                     "
BASE COUNT          778 a         1784 c         1801 g          762 t
ORIGIN      
        1 accccgccca cgagcgagcg ccaccagtgc ccggcctccc ggaaggcggc cagggtctcg
       61 gtggcgtcga aggtgcggtc ggccatggcg ggctcctggc tcaagtcgag atggcgaagc
      121 agcggtcggc cagctggccg agcgcgtagt cgtggtagag cccgtggtac atgtgccggg
      181 tcatctccgt ccaggcgagc gcctcggggg tggagatgcg gtgggcgatc tggacgatgc
      241 cgccctcgtg cacccggtac tccgcccacg tccccgggta gtccttgacg cacgccacct
      301 cgacccacgg gacgtcgccg gtgggtccga accggcggac gcggttccgg tgggtgtggc
      361 cggcggagta cgccttgatg gccggccgac gggccaccag ctcgagcagc gccgccgaat
      421 cctccaggcc gatgccgaag gtgcggtccg ccttctccgg cgagtccggg tcgcccaggt
      481 ggtggtggcc gaacagcagg atcggccggt cggcccgggc tgccacctca tcgagctgct
      541 cgagctggtc gggtgtgacc tggccgggca cgccgccgtc gatcgacgtg tcgatcacca
      601 cgagcgacac gccgggaagg tcaacctgct ggaacggctc ggccgcgaac ggcgcccggt
      661 ggtagctctc gtggttgcct cggacgacgg tgagccggtc gccgaacacc ggctcgtagg
      721 cagcccggaa cgcctcgtac tcctcgaccg tgccgttcga ggtgaggtcg cctttcacca
      781 gcacggccgc cggatcgatg gcggtcatct ccgcgatggc accgcggttc atgatctcgg
      841 ggaacgcgtc ctcacccggt tcggtccgga acacgggccc gacgtcggag ccgtcgatga
      901 gcccgcacac ctcctcgccg aagtgcacgt cgttgacggt ggcgaaggtc gtcagcagct
      961 cgccgggcgc cggcagggtg cggaacgcga acccgtccag ctcctgatcg gtgccgggcg
     1021 tcaggccgtc gtgacggcgg acctccagcc cttcgtgaac caccgcgaag tccggaccga
     1081 ccgtcgatag ctcgggaggc gaggtggcgt gggtgaccgc atccacccag tcgtcgaccg
     1141 gttccatgca gcggacccta ccggcgcgcc cgcccgccaa cccggggcgg gtgacacatg
     1201 cgatccgagc tcaatccttt ccgataggta tggatcgttc ctccggccgc tgcgcacgtc
     1261 ccggggcatg acccaggccc agctggccga gaccacgggc atcagccagc ccaacctctc
     1321 ggcctacgag aacagccggc gggagccgac gctcgacgtg ttgaaccgca tcgtcgtcgc
     1381 ctgcggctac cagctcgtcg ccgaggcggg cgccaagcag atccccgtcc ccctccccag
     1441 ggtcggctgg ttcccgctcg aagacctccc gccacggctg cccgacgatc ctcccgacga
     1501 gccgtcgacg atgccctggg acacgccgac gaacgtgcgc atcgcggcga tccacgcacg
     1561 ccaggccggg gcgggctcgt ccggtgaacg tcatcgacct cgccgtcgcg acgcatgacc
     1621 tcctcgagcg cgccgggctc ccccacgcct tcggcggcgc gctcgcgctc aacctgtgcg
     1681 ccgagccgcg gatgaccagc gacgtcgacg tgtcggtgtt cgtaccctgg gagcagcagg
     1741 acaccatcct cccgctcttc gaggagatcg ggttcgtgcc ggcaccgacg agcggaccgc
     1801 cggtacctgg cgtcggcatc cggctccgat cggaggagca ccgcgagtgc gtcgacgtgt
     1861 tcttcgcgct cgatccgatc tacgacgagg tacggatcga tcggtgccgg tcgcctaggg
     1921 acgagccggt gaagagctcc ccttcttcac tgcggaagac atcgtcatgt tcaagctgtc
     1981 gttcaaccgg cccaaggact gggtcgacgt ccagcgcgtc atccgcaacg ggccggacct
     2041 ggacacggac tccatcgagc gagtcctcgt cgagatgcga gggccgacga ggcacccgcg
     2101 cgtcgcccgt ctgcgggcga tgatccgatc gggcggcgac ctggggtagc cggagctacc
     2161 aggggcgggc gcggcgctgg gggatcgggc gggaccacca gcgaccggac cagcgccagc
     2221 ggggcggggc ggtcgacggg accggggcgg caccgcgcgg ggtggcggca tccacggcag
     2281 cgacgatggc cgccacctcc tcctcggtgg gatcacccga gatctggacc tgggtcacag
     2341 cggcatgttc ccgtgcttgc ggggcggcag ctcttccttc ttcgactcca gcatccgcag
     2401 gccgtcgatg agcatctgcc gggtgaccga cgggtcgatg acgtcgtcga cgtagccgcg
     2461 ctcggcggcc acgtacgggt tcagccactt ctccgagtag gcgtcgatga gctcggcctt
     2521 cttggcgtcc ttgtcgtcgg cctcggcgat ctccttgcgg ttcaggatct cgacggcgcc
     2581 ggtggcaccc atgacggcca gctcggccga cggccacgcg taggcgaggt cggcaccgat
     2641 ggacttggag tccatcacca cgtaggcacc gccgtaggcc ttgcgggtga tgacctggat
     2701 ccgcgggacg gtggcctcgc agtaggcgta cagcagcttg gcgccgtggc ggatgatgcc
     2761 gttgtactcc tggtcgacgc cggggaggaa cccgggcacg tcgacgaagg tcacgagcgg
     2821 gatgttgaac gagtcgcagg tccgcacgaa gcgagcggcc ttctccgagg cctcgatgtc
     2881 gagcacgccg gcgtacgccg ctggctggtt gccgaccacg ccgaccgact ggccgccgag
     2941 gcggacgaac ccgcacacga tgttggtggc ccacttcgag aagtactcga agaactcgtt
     3001 gtcgtcggcg acagcctcca cgaccttgcg catgtcgtag gggacgttgg cgctggccgg
     3061 gatgaggtcg agcagctcgg gcgtctcgcg gtcgatgggg tcgccggtgt cgatgaccgg
     3121 ggccgtctcg aggttgttga gcggcaggta cgacagcaag aagcgcacgt cgtcgaggac
     3181 ggccttgtcg tccggtgcgg tgaaggcggc gatgccggac ttcgacgcgt gggtctgggc
     3241 gccgcccagc tcctgctggg tgacgtcctc gccggtcacg gtcttcacga cgtccgggcc
     3301 ggtgatgaac atgtacgacg tgtcctccac catgaagatg aagtccgtca gcgccgggct
     3361 gtagacggcg ccgccggcgc acgggcccat gatcaccgag atctgcgggg tcacacccga
     3421 cgccatcacg ttgcggtgga agatcccgcc gtaggaagcg agcgagaccg ggccctcctg
     3481 gatgcgggcg ccggcgccgt cgttgatgcc gacgacgggt gcgcccacct tgagcgccat
     3541 gtccatgacc ttgtggatct tctcggcgaa cacctcgccg agagcgccgc cgaagagcgt
     3601 gaagtcctgg gagaacacga agaccgtgcg accctcgacc gtgccccaac cggtgatgac
     3661 accatcggtg tagggacggt ccttgacgtc ggggctgcgg tgccgggcga gcatgtccag
     3721 ctcgcggaac gagccctcgt cgagcaggta ctcgatgcgc tcacgggcga gcatcttgcc
     3781 ccggtcgtgc tggcgctgga tcgagcgctc ggagccgggg gtgcgggcct gttccttgcg
     3841 ggcgagcagg tcgtcgaggc gctcgcggat tggatgctcg ggcatggcgc agaacctacg
     3901 ccagcaccgt ttccacgacc caaccgtgac ccttgccacc acctgcattc gcccccacct
     3961 ccccgaaatc gggtcacctg ggcacctcaa cggtgcccag gtgacctgaa atcgctcgag
     4021 cacaggcttc cacgtcaggc cgacgcgaaa tcaggtcacg cgtgcacctc tgcggtgccc
     4081 aggtgacccg aaatcaaggg ggcgagcagg gcaggacgat ctgcggcagg gttgtccgat
     4141 gcccgacacg ccccggatca gcccgctccc acccgacggg cgggaccctc gaacgcaggc
     4201 gctcctccag tcgctgaagt tcgatccgga gggcgaggac atgaacctgt tcgcgaccct
     4261 cgcccaccac ccccggctgc tgaggcgctg gtcgcagttc ggcgggctgc tgctggcggg
     4321 cgggacgctc tccccgcgag atcgcgaggt cctcatcctg cgcaccgcgg ccaactgcgg
     4381 cgccggctac gagtggggcc accacctgcc catcggcgag gccgccgggc tcacgacgaa
     4441 cgagatgacg gcgctggccg ggtcccgccg ggaggccgaa ggagtcgacc aggacctcat
     4501 ccgggccgcc gacgagctcc acgaccgcaa cgtgatcatc cccgagacgt gggctgcgct
     4561 ggccgcccgc ttcgacgagc agcagctcat cgagatctgc atggtcgtcg gccagtacca
     4621 cctggtggcg ttcaccctcc gctcgctggg catccagcgg gaggccggcg tggaggggct
     4681 tcccgagtga gccggctgga gggccgcaag atcctggagg tcggagccgg cacgcgaaag
     4741 gtcgacgacc ccgacacccc ggccggcaac gggcgggcca ttgctgtcac cgccgcgtcc
     4801 gagggagcga ccgtcgcctg cgccgacatc gacctcgccg cggcgtagga gacggcggcg
     4861 caggtgcgcg acgtcggggg ccacgcccag gtgatcgtcg ccgacgtgtc cgacgccgca
     4921 gcgtgcacgg ccatggtcgc caaggccgcc gacctgctcg aggggctcga cggcgtcgtc
     4981 gtcaacgcca ccctctgacc gccccctccc cctcccccac ccccaccccc accccccccg
     5041 gaacttttcg tgatcgtggt ggctcagcca ccaacttcac gaaaagttcg tgggtgagcg
     5101 ggggtggggg tgggggtggg ggtgg
//
LOCUS       DATMFS010000395         8863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_340115, whole genome shotgun sequence.
ACCESSION   DATMFS010000395 DATMFS010000000
VERSION     DATMFS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340115"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VNQ33_07350"
     CDS             <1..499
                     /locus_tag="VNQ33_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97959.1"
                     /translation="TATLTDLQEGTYVIACFLTDADGHDHASMGMLGTVTVTGADADE
                     LPEADGTITLDDDRIELPDGFDGHGTYAVANSGTKTHGFQLAHLDGATTLEEYNTHIG
                     RSMQSSTAVDGGGGTLAGGIDSLAPGSTAYLTVDLPSGRYGYVSAPDQTGAGLPPLHG
                     ELTVS"
     gene            562..2646
                     /locus_tag="VNQ33_07355"
     CDS             562..2646
                     /locus_tag="VNQ33_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ97960.1"
                     /translation="MAPQLDSASEHRWSAWSARWSAWMVAAVVVTCTGATLELRWANR
                     HTPVSTWWQPIMAIGLGLSLLGVILLRKRPANPIGWVAAAGGIGQLLTAATREWAVFA
                     HATRGGLLPGAALAGWVGTWTYLSGAASLPLVLVLFPDGDLPSPRWRWLLRSILVIIV
                     ASIVVGALTPGPFTTEMPDLVNPVGVDLPLLLETSGALNVAMVVVTLAAAWSLLQRYR
                     RRDEVTRQQIRLVTFAGVVTAGALALSFTPIAYEGVLGMAYWLMPSCVALFVGSVAVA
                     VLRYRLWDLDVIVRLSLVYGALSILLIGIYAGLVAALTTLVGGSVAVGPSLAAATVVA
                     VAFAPVREVVQRGIDRALYGHAHDARQARIAFEGSIGRPEPVDDALDDIVSLIVTSLR
                     VDGVVLGITGQGIVASAGANRSDAHRVELCFREEDVGWLEITARAGAPLGRAGRRTLA
                     ELAPSVAATVGLVEVNAALEQSRKELLSARAEERWRIRRDLHDGLGPTLAAIAMQLDG
                     TRAVLDTTPDAAKAQLERLGDELRQAIVEIRRVVHDLQPPVLGELGLIAALADQASSF
                     SSPAAGAGLEVELRAPSAVSDVPAAVELAVYRIVGEALTNVARHAGARRCSVTVDLVD
                     AGRTLVVQIDDDGRGVADDAPAGMGTTSMVDRARELGGSCSVERSPCGGTRVRASLPL
                     DRPGHEGDRRGR"
     gene            2636..3307
                     /locus_tag="VNQ33_07360"
     CDS             2636..3307
                     /locus_tag="VNQ33_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ97961.1"
                     /translation="MDAEPIRVLIVDDHPMFRDGLRLVLEAEPTVDLVAEADDGLAAI
                     ALAGDHQPDVVVMDLNLPGCHGIEATRQIVAASPHIGVLVLTMFDDDGSVFAALRAGA
                     RGYLLKGAGQREVVRAVQAVASGQVIFGPAIARQMQHFFAAPAPVASAFPTLTPREHE
                     VLDLVASGHSNPEIARVLGVSGKTIRNHVSNLFTKLQVADRAQAIIRARAAGLGGDSG
                     SPGPP"
     gene            3429..5579
                     /locus_tag="VNQ33_07365"
     CDS             3429..5579
                     /locus_tag="VNQ33_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97962.1"
                     /translation="MDLGGDWRAAIADEDLRRTWLETDDDHDWDPVTVPGHWRSDRAF
                     ADNDAPLLYRTRFEHDGPTDPDERWWLRFDGIFYQGDVWLDGGYVGDTEGYFFPHTFE
                     VTEELAARTEHTLGVEVTCHRPGDLTAKRNITGSFQHAPYLDPASNPAGIWRPVRLER
                     TGPVRIRHVRILCRDANDRLATVAFRTVLDAATACEVEVRSTVGDRELVEHRRLAAGE
                     NQVEWTMGIDEPTLWWPHALGNQPLHDAAVEVTPLDDASNPSGDGPSHRVTRRIGLRE
                     VALKDWVLHVNGERLFLKGASQGPSNARLAEATAEDLARDVALAKDANLDLLRLHAHI
                     SRPELYAAADEAGMLIWQDFPLYRGYARSIRKQAQRQAREAVDLLAHHPSVAIWCAHD
                     EPLTIDPQAGAGLDDLTAPARYVLAQELPSWNRSILDRSVKRAIEKNDGTRPVIAHSG
                     VLPHPPQLDGTDTHVSFGWNHGDERDLAGFARALPRLVRFVSGFGAHSVPESAGFAHP
                     ESWPNLDWEALADHHGLATVVFERRLPAVDHPSFAAWRQATQDYQAELLRHHVETLRR
                     LKYRPTGGFAAVGFADSQPAVSGAVLDHERIPKAAYAALAEACRPVVVVADRLPAEVS
                     GGDTLALDVHVISDLRTPVEELEVAARLSWSGGEQRWRFGGAIAADTCARVGTLQVEV
                     PDRPGPLVLDLSLTGRGLGDDPIERTDRSRIVVG"
     gene            complement(5559..5987)
                     /locus_tag="VNQ33_07370"
     CDS             complement(5559..5987)
                     /locus_tag="VNQ33_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWJ97963.1"
                     /translation="MERWNPWAALRASSVELWFAPLEGERGRWIRHDGGDEILLDQAL
                     DRRARQEVLAHELVHVERGVGWPHATAATMEAEEERVWRTALRRLAPPAEIRRFLARR
                     GTVGPVTVADLAEEFDLSADAAARVARLYAVTGASADDDA"
     gene            complement(6029..6379)
                     /locus_tag="VNQ33_07375"
     CDS             complement(6029..6379)
                     /locus_tag="VNQ33_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ97964.1"
                     /translation="MEGDLQHIVGANVRRLRRQMGHSQESFGEHVGWHRTFVGAVERG
                     ERNLTLRTVERLAQQLSVHPLALLFDGVSAFAPVLEPGQRSRSITFASDSLDGAPPAA
                     PSAGVRRRARPRPA"
     gene            6570..7319
                     /locus_tag="VNQ33_07380"
     CDS             6570..7319
                     /locus_tag="VNQ33_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HWJ97965.1"
                     /translation="MAQTTVARGPASGGAKRAKRKAPFPVELYRSALGKKYVMAITGI
                     MLMGFVFFHMVGNLKMYMGPVHYDEYAEFLREILVPILPRTVFLWILRIGLIGAFALH
                     IHSAYSLTRMNHAARTVKYQSPRDYVAANFASRTMRWTGIIVGLFLVWHLFDLSWTGT
                     PGMTFVRGHAYANVVSSLGRPVVAAFYVAANIALGIHLFHGAWSLFQSMGWNNPRFNV
                     WRRNFAIAFAAIIVIGNVSFPLAVQFGIVSN"
     gene            7328..>8863
                     /locus_tag="VNQ33_07385"
     CDS             7328..>8863
                     /locus_tag="VNQ33_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWJ97966.1"
                     /translation="MELNSKIPEGPIAEKWTNYKFDRKLVNPANRRKYDIIVVGTGLA
                     GGAAAATLGEQGYNVKAFTFHDSPRRAHSIAAQGGINAAKNYKGDGDSIYRLFYDTVK
                     GGDFRAREANVYRLAEESVNIIDQATAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGVYSQLARQIGLGNVALYNRSELIDVVVVDGEAAGIVVRDLLTGEVQSHS
                     AHAVVLATGGYSNVFFLSTNAMNCNVTAAWRAHRRGAAFANPCYTQIHPTCIPQSDEF
                     QSKLTLMSESLRNDGRIWAPKSPDDNRPADQIPEEDRDYYLERRYPSFGNLAPRDVAS
                     RAALVEVEKGKGVGPLKNGVYLDFSDAINRLGEHVIEERYGNLFEMYERITDESPYKT
                     PMRIYPAPHYTMGGLWVDYELMTSVPGLYATGEANFSDHGANRLGASALMQGLADGYF
                     VLPNTISNYLAPKLGTTPIPTDHPEFQAAEAAARSRFEGYLAIGGTRSPDSFHHELGK
                     IMLS"
BASE COUNT         1266 a         3394 c         2963 g         1240 t
ORIGIN      
        1 caccgcgacg ctcaccgacc tgcaggaggg cacctacgtc atcgcctgct tcttgacgga
       61 cgccgacggc cacgaccatg cgtcgatggg gatgctcggc acggtcacgg tgaccggagc
      121 ggacgccgac gagctgccgg aggccgatgg aacgatcacc ctcgacgacg accgcatcga
      181 gctcccggat ggcttcgacg gccacggcac ctacgcggtg gccaactccg ggacgaagac
      241 ccacggcttc cagctggcgc acctcgacgg cgccacgacg ctcgaggagt acaacaccca
      301 catcggccgg tccatgcagt cctcgaccgc ggtcgacggc ggagggggca cgctcgccgg
      361 agggatcgac tccctggccc cgggatcgac ggcctacctc accgtcgatc tgccctcggg
      421 ccggtacggc tacgtctcgg cgcccgacca gaccggcgcc ggcctccctc cgctgcacgg
      481 cgagctcacg gtcagctgac ccgggctgcg agccggcctg agaccggggt cggccgctcc
      541 ccgaggacga gaatcgtggt gatggcccct cagctcgact ccgcatcgga gcaccgctgg
      601 tccgcgtggt ctgctcgctg gtctgcgtgg atggtcgccg ccgtcgtcgt gacgtgcacg
      661 ggcgccaccc tggagctccg gtgggccaac cggcacacgc cggtatccac ctggtggcag
      721 ccgatcatgg ccatcgggct cggactgtcg ctcctcgggg tcatcctgct ccgcaagcgc
      781 ccggcgaacc cgatcggctg ggtcgcagct gccggcggca tcggccagct gctcaccgcc
      841 gcgaccaggg agtgggcggt cttcgcccac gccacccgag gcgggctcct ccctggtgcc
      901 gcgctcgccg gctgggtcgg cacgtggacc tacctgagcg gcgcggccag cctccccctc
      961 gtgctcgtcc tcttcccgga cggcgatctg ccgtcacctc ggtggcggtg gctgctgcgg
     1021 tcgatcctcg tcatcatcgt cgccagcatc gtcgtcgggg ccctgacccc cggacccttc
     1081 accaccgaga tgcccgatct ggtgaacccg gtcggggtcg acctgccgct gctcctggag
     1141 acctccggcg cactgaacgt cgcgatggtc gtggtgacgc tcgccgccgc gtggtcgctc
     1201 ctccagcgct accgaaggcg agacgaggtg acccgccagc agatccgctt ggtcaccttc
     1261 gccggcgtgg tgaccgcagg ggcgctcgcg ctcagcttca cgcccatcgc ctacgaaggc
     1321 gtgctcggca tggcctactg gctcatgccg agctgcgtcg cgctgttcgt cggcagcgtc
     1381 gccgtcgccg tcttgcgcta ccggctctgg gacctcgacg tgatcgtccg gctctcactc
     1441 gtgtacggag cactgtcgat cctgctgatc ggcatctacg ccgggctggt cgctgcgctc
     1501 accaccctgg tcggcggatc ggtcgccgtc ggtccgtcgc tcgctgcggc caccgtcgtc
     1561 gcggtcgcct tcgcgccggt gcgcgaggtc gtgcagcgcg gcatcgaccg tgccctctac
     1621 ggccacgcgc acgacgcgag gcaggcccgg atcgcgttcg aggggagcat cggccggccc
     1681 gaacccgtcg acgacgccct cgatgacatc gtctcgctga tcgtcacgtc gctgcgagtc
     1741 gacggggtgg tgctggggat cacgggccag gggatcgtcg ccagcgcggg agcgaaccgc
     1801 tcagatgccc atcgggtcga gctgtgcttc cgggaggagg acgtcggctg gctcgagatc
     1861 acggcccgtg ctggtgcacc gctcgggcgc gccggtcgcc gcacgctggc cgagctggct
     1921 ccttcggtcg ctgccaccgt cggcctcgtc gaggtgaacg ccgcgctcga acagtcccgc
     1981 aaggagctcc tctccgcgcg cgccgaggag cgctggcgga tccgacgaga cctccacgac
     2041 ggtctcggtc ccacactggc cgccatcgcc atgcagctcg acggcacccg ggccgtgctg
     2101 gacacgacgc ccgacgcggc caaggcgcag ctcgaacggc tcggcgacga gctgcgccag
     2161 gcgatcgtgg agatccgccg ggtcgtccac gacctccagc cgcccgtgct cggcgagctc
     2221 ggcctgatcg ccgcgctcgc cgatcaggcg tcgtcgttct catcccccgc ggctggggcg
     2281 ggcttggagg tggagctccg cgctccctcc gccgtctcgg acgttcccgc agcggtggag
     2341 ctcgcggtgt accgcatcgt cggcgaggcc ctgacgaacg tcgcgcgcca cgccggcgcg
     2401 cgacgatgca gcgtgaccgt ggacctcgtc gatgccggcc ggaccctcgt cgtgcagatc
     2461 gatgacgacg gcaggggcgt cgcagacgat gcccccgcgg ggatgggcac cacctcgatg
     2521 gtcgaccggg cccgcgagct cggcggctcg tgctcggtcg aacggtcgcc ctgcggcggg
     2581 accagggtcc gggcgagtct tcctctcgac cggcctggcc acgaggggga tcgacgtgga
     2641 cgctgagccg atccgcgtcc tgatcgtcga cgaccacccg atgttccgag acgggctgcg
     2701 cctggtgctc gaggcggagc cgacggtgga cctggtcgcc gaggcggacg acggcctcgc
     2761 ggccatcgcg ctcgccggcg accaccagcc cgacgtggtc gtcatggacc tcaacctgcc
     2821 tggctgccac ggcatcgagg ccacccgcca gatcgtggcc gccagccccc acatcggggt
     2881 gctcgtcttg accatgttcg acgacgacgg aagcgtcttc gccgcgctgc gggcgggtgc
     2941 gcgcggctac ctgctgaagg gcgccggcca gcgcgaggtc gtccgggcgg tccaagccgt
     3001 ggcgtccggc caggtcatct tcggtcccgc catcgcccgc cagatgcagc acttcttcgc
     3061 ggccccggcg ccggtggcga gcgcgttccc cacgctcacg ccccgcgagc acgaggtgct
     3121 ggacctggtg gcttccggac actcgaaccc ggagatcgcc cgcgtcctgg gggtgtcggg
     3181 caagacgatc cgcaaccacg tgtccaacct cttcaccaag ctccaggtgg ccgaccgggc
     3241 ccaggccatc atccgggccc gggcggcggg cctgggcggc gacagtggct cgccggggcc
     3301 gccctgaccc gatgccgacc ggccaccggc ggacaccgcc gccgcggccg ggtcatcacc
     3361 cggccccggc cacgcgggac ggccccgccg gcgagagggc ggcgggcgga ccggtagcgt
     3421 cggagggcat ggacctcggg ggcgactggc gggcggcgat cgccgacgag gacctgcggc
     3481 gcacctggct cgagaccgac gacgaccacg actgggatcc ggtcaccgtc ccggggcact
     3541 ggcgcagcga ccgcgccttc gccgacaacg acgcgccgct cctctaccgg acccggttcg
     3601 agcacgacgg ccccacggac cccgacgagc gctggtggct gcgcttcgac gggatcttct
     3661 accagggcga cgtctggctc gacggcggct acgtgggcga caccgagggc tacttcttcc
     3721 cccacacgtt cgaggtcacc gaggagctcg ccgcccgcac ggagcacacg ctcggcgtcg
     3781 aggtcacgtg ccaccgcccc ggcgacctga cggccaagcg caacatcacc ggctcgttcc
     3841 agcacgcccc gtacctcgac ccggccagca acccggcggg catctggcgg cccgtgcgcc
     3901 tcgagcgcac cggaccggtg cgcatccgcc acgtccgcat cctctgccgc gacgccaacg
     3961 accggctggc caccgtcgcc ttccggaccg tgctggacgc cgccaccgcc tgcgaggtcg
     4021 aggtgcgctc gaccgtcggc gaccgggagc tggtggagca ccggcggctc gccgccggcg
     4081 agaaccaggt ggagtggacg atgggcatcg acgagcccac gctctggtgg ccgcacgcgc
     4141 tcgggaacca gccgctccac gacgccgccg tcgaggtcac gccgctcgac gatgcgtcga
     4201 acccgtcggg cgatggcccg agccaccggg tcacccggcg catcggcctc cgcgaggtcg
     4261 ccctcaagga ctgggtgctc cacgtcaacg gcgagcgcct cttcctcaag ggggcgagcc
     4321 agggcccgag caacgcccgc ctggccgagg ccacagccga ggacctagcg cgcgacgttg
     4381 cgctcgccaa agacgccaac ctcgacctgc tccgcctcca cgcccacatc agccggcccg
     4441 agctctacgc cgcggccgac gaggcgggga tgttgatctg gcaggacttc ccgctctacc
     4501 gcggctacgc ccgcagcatc cgcaagcagg cccagcgcca ggcccgcgag gcggtcgacc
     4561 tgctggccca ccacccctcg gtcgcgatct ggtgtgcgca cgacgagccg ctcaccatcg
     4621 atccccaggc cggcgccggg ctcgacgacc tcaccgcgcc ggcccgctac gtgctggccc
     4681 aggagctgcc gtcttggaac cggtcgatcc tcgaccggtc cgtgaagcgg gccatcgaga
     4741 agaacgacgg cacccggccc gtcatcgccc actccggcgt gctcccccac ccgccccagc
     4801 tcgacggcac cgacacccac gtgtcgttcg gctggaacca cggcgacgag cgcgacctcg
     4861 ccgggttcgc ccgggccctc ccgcgcctcg tgcggttcgt gtccggcttc ggtgcccact
     4921 cggtgcccga gtccgccggc ttcgcccacc cggagtcctg gccgaacctc gactgggagg
     4981 ccctggccga ccaccacggc ctggccacgg tggtgttcga gcgccggctg cccgccgtgg
     5041 accacccctc gttcgcggcg tggcgccagg cgacgcagga ctaccaggcc gagctcctcc
     5101 gccaccacgt cgagacgctc cgccggctca agtaccggcc caccggcggg ttcgccgccg
     5161 tcgggttcgc cgacagccaa ccggcggtct ccggcgccgt gctcgaccac gagcggatcc
     5221 cgaaggccgc ctacgccgcc ctcgccgagg cgtgccggcc ggtggtcgtg gtggccgacc
     5281 gcctgccggc cgaggtgtcc ggcggcgaca cgctcgccct cgacgtgcac gtgatcagcg
     5341 acctgcgcac ccccgtggag gagctcgagg tggccgcccg cctgtcgtgg tcgggcggcg
     5401 agcagcgctg gcgcttcggc ggggccatcg ccgccgacac ctgtgcccgc gtcggcacgc
     5461 tccaggtgga ggtcccggac cgtcccggtc cgctggtgct cgacttgtcg ctcacgggcc
     5521 ggggcctcgg cgacgacccc atcgagcgca ccgaccgctc acgcatcgtc gtcggctgac
     5581 gcacccgtca cggcgtagag ccgggcgacg cgcgccgcgg catcggccga caggtcgaac
     5641 tcctcggcca ggtccgcgac cgtcacgggg ccgaccgtgc cgcgccgggc gaggaagcgg
     5701 cggatctccg ccggcggggc gagccggcgc agggcggtcc gccacacccg ctcctcctcg
     5761 gcctccatgg tggcggcggt ggcgtgcggc cagccgacgc cgcgctcgac gtgcaccagc
     5821 tcgtgggcca ggacctcctg gcgcgccctg cggtccagtg cctggtccaa gaggatctcg
     5881 tccccgccgt cgtggcggat ccaccggccc cgctcgccct ccaggggagc gaaccacagc
     5941 tcgaccgacg acgcccgcag tgcggcccag gggttccatc gctccaccgg tccagtatcg
     6001 atcggggctc tgacagcgga cggtgccgtc acgctggccg cgggcgcgct cgccgccgca
     6061 ccccggctga cggcgcggcc ggcggcgcgc cgtcgagcga gtcggaggcg aaggtgatcg
     6121 agcgcgaccg ctggcccggc tcgagcaccg gggcgaacgc cgagacaccg tcgaacagca
     6181 gggcgagcgg gtgcacgctc agctgctggg cgaggcgctc gaccgtccgc aaggtgaggt
     6241 tccgctcgcc ccgctccact gctccgacga acgtccggtg ccatccgacg tgctcgccga
     6301 aggattcctg cgagtgcccc atctgccgcc tcagtcgtcg gacgttggcc ccgacgatgt
     6361 gttggaggtc accttccatg cgcaccacct tcgatgacgg cgccctcgga atcgacagac
     6421 taatgagtgg cagcaccacc tgtgacctgc ggttttgatg tccgattttg cgccgggggc
     6481 cgcgacggcg ggtcggcccg cgaacagacc ggagcgaccc gacccagcgt ttgttccgcg
     6541 aggtgcgacc aggggtagcg tcgagagcga tggctcaaac aaccgttgcc cgaggccccg
     6601 ccagcggcgg ggccaagcgg gccaagcgca aggccccctt ccccgtcgag ctctaccgct
     6661 cggcgctggg caagaagtac gtgatggcga tcaccggcat catgttgatg gggttcgtct
     6721 tcttccacat ggtcggcaac ctcaagatgt acatggggcc cgtccactac gacgagtacg
     6781 ccgagttcct gcgggagatc ctggtcccga tcctgccccg gaccgtgttc ttgtggatcc
     6841 tgcggatcgg gctcatcggg gcgttcgcgc tgcacatcca cagcgcctac tcgctcaccc
     6901 gcatgaacca cgcggcccgc acggtgaagt accagtcgcc gcgcgactac gtggcggcca
     6961 acttcgcgag ccgcacgatg cggtggaccg gcatcatcgt cggcctcttc ttggtctggc
     7021 acctgttcga cctgagctgg acgggcaccc ccgggatgac cttcgtccgg ggccacgcct
     7081 acgccaacgt cgtcagcagc ctgggccgcc cggtggtcgc cgccttctac gtggccgcca
     7141 acatcgccct gggcatccac ctcttccacg gagcgtggag cctcttccag tcgatggggt
     7201 ggaacaaccc ccgcttcaac gtgtggcgcc ggaacttcgc catcgccttc gccgccatca
     7261 tcgtgatcgg caacgtctcg ttcccgctgg ccgtccagtt cggcatcgtg agcaactagg
     7321 agccactgtg gaactcaact cgaagatccc cgagggcccc atcgccgaga agtggacgaa
     7381 ctacaagttc gaccgcaagc tcgtgaaccc ggccaaccgc cgcaagtacg acatcatcgt
     7441 cgtcggcacc gggctggccg gcggtgcggc cgccgccacg ctcggcgagc agggctacaa
     7501 cgtgaaggcc ttcaccttcc acgactcgcc ccgccgggcc cactcgatcg ccgcccaggg
     7561 cggcatcaac gccgccaaga actacaaggg cgacggcgac tcgatctacc gcctcttcta
     7621 cgacaccgtg aagggcggcg acttccgggc ccgtgaggcc aacgtgtacc gcctggccga
     7681 ggagtcggtg aacatcatcg accaggccac ggcccagggc gtcccgttcg cccgcgagta
     7741 cggcgggctg ctcgacaacc ggtcgttcgg cggcgcccag gtctcccgca ccttctacgc
     7801 ccggggccag accggccagc agctcctcct cggcgtctac tcgcagctcg cccgccagat
     7861 cggcctcggc aacgtggccc tctacaaccg cagcgagctc atcgacgtgg tcgtcgtcga
     7921 cggcgaagcg gccggcatcg tcgtccgaga cctgctcacc ggcgaggtcc agagccactc
     7981 ggcccacgcc gtggtgctgg ccaccggcgg ctactcgaac gtgttcttcc tctcgacgaa
     8041 cgccatgaac tgcaacgtga ccgccgcctg gcgggcccac cgccgggggg cggcgttcgc
     8101 caacccctgc tacacgcaga tccacccgac ctgcatcccc cagagcgacg agttccagtc
     8161 gaagctcacc ctcatgtcgg agtcgctccg caacgacggc cgcatctggg ctccgaagtc
     8221 gcccgacgac aaccggcccg ccgaccagat ccccgaggag gaccgggact actacctcga
     8281 gcgccgctac ccgagcttcg gcaacctcgc cccgcgcgac gtcgccagcc gcgccgccct
     8341 ggtggaagtc gagaagggca agggcgtcgg ccccctcaag aacggcgtct acctggactt
     8401 ctccgacgcc atcaaccgcc tgggcgagca cgtcatcgag gagcgctacg gcaacctctt
     8461 cgagatgtac gagcgcatca ccgacgagtc gccgtacaag acgccgatgc gcatctaccc
     8521 cgccccgcac tacacgatgg gcgggctctg ggtggactac gagctcatga cctcggtccc
     8581 cgggctctac gccacgggcg aggccaactt ctcggaccac ggcgccaacc gcctgggggc
     8641 ctcggcgctc atgcagggcc tggccgacgg ctacttcgtg ctgccgaaca ccatcagcaa
     8701 ctacctggcc ccgaagctcg gcaccacccc gatccccacg gaccacccgg agttccaggc
     8761 agccgaggcg gcggccagga gccgcttcga gggctacctc gccatcggcg gcacccggtc
     8821 gcccgattcc ttccaccacg agctgggcaa gatcatgctc agc
//
LOCUS       DATMFS010000396         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_316808, whole genome shotgun sequence.
ACCESSION   DATMFS010000396 DATMFS010000000
VERSION     DATMFS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316808"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="groL"
                     /locus_tag="VNQ33_07390"
     CDS             <1..1422
                     /gene="groL"
                     /locus_tag="VNQ33_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055846.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWJ97967.1"
                     /translation="VKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPMGLK
                     KGIEIAVEAISDQLLAMAKDVETKEQIASTASISAADTTVGEIIAEAMDKVGKEGVIT
                     VEESNTFGLDLELTEGMRFDKGHLSGYFVTDPERMETVLEDPYILIVNSKISTVKDMV
                     PVLEKVMQTGKPLAIIAEDVEGEALATLIVNKLKGTFKSVAIKAPGFGDRRKAMLTDI
                     AILTGGEVISEEVGLKLENADLTLLGTARKVVTSKDETTIVEGGGDEAQIQGRVAQIK
                     AEIENSDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKERKHRIEDAVRNAKAAVE
                     EGIVPGGGVALVQASKAAFEKLELEGDEATGANIVRVATSAPLKQIAVNAGLEGGVVA
                     EKVANLEQGQGLNAATGEYVDMIATGIIDPAKVTRSALANAASIAALFLTTEAVVADK
                     PEPAAPAGAPDMGDMGGMGGMGF"
     gene            1501..2241
                     /locus_tag="VNQ33_07395"
     CDS             1501..2241
                     /locus_tag="VNQ33_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97968.1"
                     /translation="MTTWPDLSRTDDALTPEHRDRLVAAGVDPERLDAVAWRRMDGPL
                     PSWWNDLGNALYAAPGARLPDHVLAGLTTFYYSGALLVVGAPMDHLTSLLVGGDAATV
                     FLDERVVLTAGELYCGPGSSIALHGPLVATRSPVVDARNGGRVVAAGDQLWAAGVYIA
                     TDDMHRLEDAATGERLNPFGATIRIGHHVWLCREAVVSGHVEIGAHVCVGMRSVVRGQ
                     KVPPNVVVMGSPGRVVREGTTWSFDDTP"
BASE COUNT          489 a          996 c         1020 g          401 t
ORIGIN      
        1 gtcaaggaag tcgccaagaa gaccgacgac gtcgccggtg acggcaccac gacggccacc
       61 gtcctggccc aggcgctcgt gcgcgagggc ctgcgcaacg tggccgccgg cgccaacccg
      121 atgggcctga agaagggcat cgagatcgcc gtcgaggcca tcagcgacca gctgctcgcc
      181 atggcgaagg atgtcgagac caaggagcag atcgcctcca ccgcctccat ctcggccgcg
      241 gacaccacgg tcggcgagat catcgccgag gccatggaca aggtcggcaa ggaaggcgtc
      301 atcacggtcg aggagtccaa caccttcggg ctggacctcg agctcaccga gggcatgcgc
      361 ttcgacaagg gccacctgtc gggctacttc gtcaccgatc ccgagcgcat ggagaccgtg
      421 ctcgaggatc cgtacatcct catcgtcaac agcaagatca gcaccgtgaa ggacatggtc
      481 ccggtgctgg agaaggtcat gcagaccggc aagccgctgg cgatcatcgc cgaggacgtc
      541 gagggcgagg cgctggccac cctcatcgtc aacaagctga agggcacctt caagtcggtc
      601 gccatcaagg cccccggctt cggtgaccgc cgcaaggcga tgctgaccga catcgcgatc
      661 ctcaccggtg gcgaggtcat cagcgaggag gtcggcctca agctcgagaa cgccgatctc
      721 acgctgctgg gcaccgcccg caaggtcgtc acgtccaagg acgagaccac catcgtcgag
      781 ggtggcggcg acgaggccca gatccagggc cgtgtcgccc agatcaaggc cgagatcgag
      841 aacagcgact cggactacga ccgcgagaag ctccaggagc gcctcgccaa gctggccggt
      901 ggcgtggccg tcatcaaggt cggcgcggcc accgaggtcg agctcaagga gcgcaagcac
      961 cgcatcgagg acgccgtccg caacgcgaag gcggccgtcg aggagggcat cgtccccggc
     1021 ggcggtgtcg cgctcgtcca ggccagcaag gccgcgttcg agaagctcga gctcgagggc
     1081 gacgaggcca ccggcgcgaa catcgtccgc gtcgccacct cggccccgct gaagcagatc
     1141 gccgtcaacg ccggcctcga gggcggcgtc gtcgcggaga aggtcgccaa cctggagcag
     1201 ggtcagggcc tcaacgcggc caccggcgag tacgtcgaca tgatcgcgac cggcatcatc
     1261 gacccggcca aggtcacgcg ctccgcgctg gcgaacgccg cgtcgatcgc cgcgctgttc
     1321 ctcaccaccg aggccgtcgt ggccgacaag ccggagcccg cggccccggc cggtgcgccc
     1381 gacatgggcg acatgggtgg catgggcggc atgggcttct gagccacgcc tgatcacagg
     1441 accccggtac cgcgcggtgc cggggtcctg tgcgtccggg gggatgggag gatgaccggc
     1501 gtgaccacgt ggcccgacct gtcccgcacc gacgacgcgc tgacccccga gcaccgggac
     1561 cggctcgtcg cggcgggcgt cgaccccgag cggctcgacg ccgtcgcctg gcgccggatg
     1621 gacggtccgc tgccgtcgtg gtggaacgac ctcggcaacg cgctgtacgc cgctcccggg
     1681 gcccggctgc ccgaccacgt cctggccggc ctgacgacct tctactactc cggcgcgctc
     1741 ctcgtggtgg gggcgccgat ggaccacctg acctcgctgc tggtcggcgg cgacgccgcc
     1801 acggtgttcc tcgacgagcg cgtcgtgctg accgccggcg agctgtactg cgggcccggc
     1861 tcctcgatcg cgctgcacgg acccctcgtg gccacccggt cgccggtcgt ggacgcgcgc
     1921 aacgggggcc gcgtcgtggc cgccggcgac cagctgtggg ccgcgggcgt ctacatcgcc
     1981 accgacgaca tgcaccgcct cgaggacgcg gccacggggg agcggctcaa cccgttcggc
     2041 gccacgatcc gcatcggtca ccacgtgtgg ctgtgccgtg aggccgtggt gtccggacac
     2101 gtcgagatcg gcgcgcacgt gtgcgtcggc atgcggtccg tcgtgcgcgg tcagaaggtg
     2161 cctccgaacg tggtcgtgat ggggtctccg gggcgtgtgg tgcgtgaggg gaccacgtgg
     2221 tcattcgacg acacgccgtg aggcgcgatt ccgggactca ttgcacgaaa cttccagtta
     2281 caccaggtgc ccgtgcccga ttcgtcgacg tgcgaccgca cgggaacccg taaaccgcag
     2341 gacgagatgg tgtgacatgg acgacatccg cgaatttctg aaatgaccag acagccgttc
     2401 gacgatggcc tactttcgag gggaaagcag gggcgtcgtg ccagtcgatg gccgccggcg
     2461 ggggaagccg gcgagggaga gcatcgagtg gcctggagcc gggccggggg gcttgtccac
     2521 ggacatgtat cggtaccact ccacgtcgct cttggctgag tacgcagaca cagacagcac
     2581 gtgggtcggc ggtgaggcgg aggggtccgc agctcgacac cgagggctcg cattggggag
     2641 tggcaatgac ttcagcgatc gacggacgag atgagaccgt ccttccgctg cgtctggtgg
     2701 agcccatcac cagcgacccc ggactcggat ctccttccgt gcaccgggaa ctcacggcga
     2761 tccccacggg gcgatcgtca ctggagaacc tgatccgcca gggcctcttc ggacacctgc
     2821 tcgtgctgac gggcaccgtc atcgccggcg tggccgtcgg catggccccc ggagcgctcg
     2881 cgttcctggc cgccggcgtc gtcgcc
//
LOCUS       DATMFS010000397         4951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_267643, whole genome shotgun sequence.
ACCESSION   DATMFS010000397 DATMFS010000000
VERSION     DATMFS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267643"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="queC"
                     /locus_tag="VNQ33_07400"
     CDS             complement(<1..651)
                     /gene="queC"
                     /locus_tag="VNQ33_07400"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWJ97969.1"
                     /translation="MDSSFAISEVLLPMSKPGVVLLSGGLDSSTVVALAQRDGYDLHA
                     LSFSYGQRHTVELEAAKRVAAAAGIADHRIIEIDLGAFGGSALTDASIDVPKHASADD
                     VEEGIPATYVPARNTVFLSFALAFAEVLGSSDIFIGVNAVDYSGYPDCRPEFVAAFQD
                     LARIATKTGVEGSTEVTIHAPLMEMTKAQIAAAGTELGVDYSITTSCYDADSEGRAC"
     gene            complement(685..2640)
                     /locus_tag="VNQ33_07405"
     CDS             complement(685..2640)
                     /locus_tag="VNQ33_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97970.1"
                     /translation="MAATERLGEVADQLDAERFVGRQRALAAIAAGRSGHTPQRIFHV
                     HGPGGVGKSALLRAAARQARHDGQLVVQLDGRLVMPTPEGLVAAVGQAGDGTLLLIDE
                     ADELLTLRFELRHLLRTAIPASAVVVLAGRTAPGREWFDEGLDQVSATIVVRPLDNTE
                     GRDLLGRCGVTEPADVDQLLTWASGYPLALTVGANLLGAPQEPASSNGRMGTASGEGL
                     DDLILGRLGGREVADVDPDVLDVACVASAVDARLLAAVLPGRPTRSGLAQLRSLSVSE
                     QLGSRTTLHRLVRSALRSRLRESDPDRYRTIVLRIAGHLRERALTEGPMIALELADLV
                     ENPEVRLGFDPSTSHYADFPRPGDLEELAEATGAGGAAWFARFRRWCEQQPRQAIAVR
                     RATGELAAMSIVCMASEMPAWADDDIETGPVLRHLRDAGHFDQAALMHDTAILEDPAD
                     VAACAEVIRVGNAGAMAGAFGARNPRYTYVTASRWNEYDGTAPLGYQEVVELRRGDDE
                     RELQTIMTDFGPEGVTGQMYAIILAEQQAEAPASATTATALALLDAVRSFHDDAALAA
                     SPLAPAEGTDAAKAAAVRTLVADGLIEAFGESETDQRLRQAIERTYLDPDGGHGIAQR
                     ELHMSRSSFYRHLQKARQQLVDHAPRR"
     gene            complement(2683..>4951)
                     /locus_tag="VNQ33_07410"
     CDS             complement(2683..>4951)
                     /locus_tag="VNQ33_07410"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HWJ97971.1"
                     /translation="PPRPGPGDGASAATAGASCFGILRAFPTSPSGTYWLSTPAMDRP
                     AQFYCDMKTDGGGWVLIGRGRQGWNWSPNGQGGTAAVRTNVSGPSAFAPAALADATIS
                     GLINGASPAALSDGIRVERATNTAGTANQQVRMFPTFTTWSWKWDGGQLLKRIVIDGT
                     SYTGSNTRDTYEKAIAGQTTNQLAGQQGLKRLFTWDWASNDGLMGFSYGKGAPKGSTS
                     STSNLWQRSSASYPIPFTRVWLRPRLANDLTYPAAPAGGYAAAPKPPGLKDRSELAPW
                     GVTGLNHADEQNVEPWSTNVLAIESTSDRVYVGGRFTGVKQGPTGATTAQSALAAFDL
                     DGNWISTFRPVVVGRVWDIALTPDNKLIIAGDFTSVNGVANARGLAALDPTTGAVIPG
                     WKARVSRVSGTEWRVRSLDVRGSWIYAAGTFDRVVAGASTNPVAVTNAMSISAADGSL
                     GAWKPTPNGSVVDIAVTNDSTRVLLAGHFTSVGGSAIHANFAITSLANGAPVSGAAAW
                     KPSDPASRYQQAVADLGDRLLVGGAQHDTQVWNKARTTLLDASITKPGGDTQVIEVVG
                     SKAYVGCHCGGWIYQGTNVFPMPPSFRAIDSINLIGAWDTATWTYDTSWFPSSLKGAS
                     GEGVWAIEPDVRGCLWVGGDLNRGAYSGVAATDWLGGFARFCPLDATTPTPPGGFSVT
                     STGLSRKVAWTAATDGTGVTSYDVIRNGRVIATVPGATRNYTDASAPAGTSYTVRAVD
                     ARGNRSASPAPVTVR"
BASE COUNT          647 a         1638 c         1949 g          717 t
ORIGIN      
        1 gcacgccctt ccctcgctgt cggcgtcgta gcagctggtg gtgatcgagt agtcgacacc
       61 gagctcggtg ccggccgccg cgatctgggc cttggtcatc tccatcagcg gggcgtggat
      121 ggtgacctcg gtgctgccct cgacgcccgt cttggtggcg atgcgggcca ggtcctggaa
      181 ggcggcgacg aactcggggc ggcagtccgg gtagccggag tagtcgacgg cgttgacgcc
      241 gatgaagatg tccgagctgc ccaggacctc ggcgaacgcc agggcgaacg acaagaagac
      301 ggtgttgcgg gccggcacgt aggtggccgg gatgccctcc tccacgtcgt cggcggaggc
      361 gtgcttgggg acgtcgatcg acgcgtcggt gagcgcggac ccgccgaacg cgcccaggtc
      421 gatctcgatg atccgatggt cggcgatgcc cgccgccgcc gccacccgct tggcggcctc
      481 gagctcgacg gtgtgccgct gcccgtacga gaacgacagc gcgtgcaggt cgtacccgtc
      541 ccgctgcgcc agggccacga ccgtcgacga gtccagcccc ccggagagca gcacgacacc
      601 aggcttggac atgggcagca ggacctcgga gatggcgaac gaggagtcca atctacgagg
      661 tggccgccac gtctcctcga tcgatcagcg gcgaggggcg tggtcgacga gctgctggcg
      721 ggccttctgg aggtggcggt agaagctcga cctgctcatg tggagctctc gttgggcgat
      781 gccgtggccg ccgtcggggt cgagataggt gcgctcgatc gcctggcgga ggcgctggtc
      841 ggtctcggat tcgccgaacg cctcgatgag gccgtcggcg accagggtgc ggacggcggc
      901 ggccttggcg gcgtcggtgc cctcggcggg tgcgagggga ctggcggcga gggcggcatc
      961 gtcgtggaag gagcggacgg catcgaggag ggcgagggcc gtggcggtgg tggcgctcgc
     1021 cggcgcctcg gcctgctgct cggcgaggat gatggcgtac atctggccgg tcaccccttc
     1081 ggggccgaag tcggtcatga tcgtctggag ctcgcgctcg tcgtcgccgc ggcgcagctc
     1141 gacgacctcc tggtagccca gaggagcggt gccgtcgtac tcgttccacc gggaggcggt
     1201 cacgtaggtg tacctcgggt tccgagcgcc gaacgctccg gccatggccc cggcgttgcc
     1261 gacccggatc acctcggcgc aggcggcgac gtcggccggg tcctcgagga tggcggtgtc
     1321 gtgcatgagc gccgcctggt cgaagtgacc agcatcacgg aggtgccgca gcaccggtcc
     1381 ggtctcgatg tcgtcgtcgg cccacgccgg catctccgag gccatgcaga cgatcgacat
     1441 ggccgcgagc tcccccgtgg ctcgtcgcac ggcgatggcc tggcggggct gctgctcgca
     1501 ccagcggcgg aagcgggcga accacgccgc gccaccggca ccggtcgcct cggccagctc
     1561 ctcgaggtcg cctggacggg ggaagtcggc gtagtggctg gtgctggggt cgaacccgag
     1621 ccggacctcg gggttctcga ccaggtcggc cagctcgagg gcgatcatcg ggccctcggt
     1681 gagcgcccgc tcgcggaggt ggcccgcgat gcgcaacacg atcgtgcggt agcggtccgg
     1741 atcgctctct cggagccggg agcgcagcgc gctgcgcacc aggcggtgca gcgtggtccg
     1801 ggatccgagc tgctccgata ccgagagcga ccggagctgg gccaggccgg agcgggtcgg
     1861 ccgaccgggc agcacggcgg cgagcagacg ggcatcgacg gccgatgcca cgcacgcgac
     1921 gtcgagcacg tcggggtcga cgtcggcgac ctcgcggccg ccgagccggc ccaggatcaa
     1981 gtcgtcgagc ccctcgccgg acgccgtgcc catccggccg ttgctcgacg ccggctcctg
     2041 cggtgctccg aggaggttgg cgccgacggt gagcgcgagc gggtatcccg atgcccaggt
     2101 gagcagctgg tccacgtcag cgggctcggt gaccccgcag cggccgagga ggtctcgtcc
     2161 ctcggtgttg tccagggggc gcacgacgat ggtggccgac acctggtcga ggccctcgtc
     2221 gaaccattcc cgccccggtg cggtccgccc ggccagcacc accacggccg acgccgggat
     2281 cgcggtgcgc agcaggtggc gcagctcgaa ccggagggtg agcagctcgt cggcctcgtc
     2341 gatcagcagc agggtgccgt cgccggcctg ccccaccgcg gcgacgaggc cctcgggggt
     2401 cggcatcacc aggcgtccgt cgagctggac gacgagctgg ccgtcgtggc gggcctggcg
     2461 ggcggcggca cggagcagcg cgctcttccc gacgccgcct gggccgtgca cgtggaagat
     2521 ccgctggggt gtgtggccgg agcggcccgc ggcgatggcg gcgagcgcac gctgccggcc
     2581 gacgaagcgc tcggcgtcga gctggtcggc cacctcgccc agacgctccg tcgcagccac
     2641 gggcgaaacg taccagtgcg gatgtccggc cgacgctgag cgtcagcgga cggtcaccgg
     2701 ggccggggag gcgctgcggt tgccgcgggc gtcgaccgct cggacggtgt agctcgtgcc
     2761 ggcgggagcg ctggcatcgg tgtagttgcg cgttgcgccg gggaccgtgg cgatgacgcg
     2821 cccgttgcgg atcacgtcgt agctggtcac cccggtgccg tcggtggccg ccgtccaggc
     2881 caccttgcgg ctcagcccgg tgctggtgac ggagaacccg ccggggggcg tgggggtggt
     2941 ggcgtcgagc gggcagaacc gggcgaagcc gccgagccag tccgtcgccg ccacgccgct
     3001 gtaggcgccg cggttcaggt cgccgccgac ccagaggcag ccgcggacgt cgggttcgat
     3061 ggcccagacg ccctcaccgg acgccccctt gagcgaggag gggaaccagg aggtgtcgta
     3121 ggtccacgtc gccgtgtccc aggcgccgat caggttgatc gagtcgatcg cccggaagct
     3181 cggaggcatg gggaagacgt tggtgccctg gtagatccac ccgccgcagt ggcagcccac
     3241 gtacgccttg gagccgacga cctcgatcac ctgcgtgtcg ccgccgggct tggtgatcga
     3301 cgcgtcgagc agcgtcgtcc gagccttgtt ccagacctgc gtgtcgtgct gggcgccccc
     3361 gacgaggagc cggtcgccga ggtcggccac ggcctgctgg tacctcgacg cggggtccga
     3421 gggcttccag gccgctgcgc ccgagaccgg ggcgccgttc gcgagcgagg tgatggcgaa
     3481 gttggcgtgg atggcgctgc cgccgaccga ggtgaagtgg ccggccagca ggacgcgggt
     3541 gctgtcgttc gtcacggcga tgtcgacgac ggacccgttc ggggtgggct tccaggcgcc
     3601 gagggacccg tcggccgcgc tgatgctcat ggcgttggtg accgcgaccg ggttggtcga
     3661 ggcgccggcg acgacgcggt cgaacgtgcc ggctgcgtag atccacgatc cgcggacgtc
     3721 gagggaccgc acgcgccact cggtgccgct gacgcgggac acgcgggcct tccagccggg
     3781 gatgaccgcc ccggtggtgg ggtcgagcgc ggcgaggcca cgggcgttgg ccacgccgtt
     3841 cacgctggtg aagtcgccgg cgatgatgag cttgttgtcc ggggtgagcg cgatgtccca
     3901 gacgcggccg acgaccaccg gacggaaggt gctgatccag ttgccgtcga ggtcgaacgc
     3961 ggcgagcgcg ctctgcgcgg tggtggcgcc ggtcgggccc tgcttcacgc cggtgaagcg
     4021 gccgccgacg tagacccggt cgctggtcga ctcgatggcc aagacgttgg tgctccacgg
     4081 ctcgacgttc tgctcgtcgg cgtggttcag gcccgtcacg ccccacggcg cgagctcgga
     4141 ccggtccttc aggccggggg gtttgggtgc ggccgcgtag ccgcccgccg gggcagcggg
     4201 atacgtgagg tcgttggcga gccggggtcg cagccacacg cgggtgaagg ggatggggta
     4261 cgacgccgac gaccgctgcc acaggttcga ggtcgagctg gtcgagccct tcggggcgcc
     4321 cttgccgtag ctgaagccca tgaggccgtc gttgctcgcc cagtcccagg tgaagaggcg
     4381 cttgaggccc tgctggccgg cgagctggtt cgtggtctgc ccggcgatgg ccttctcgta
     4441 ggtgtcgcgg gtgttggacc cggtgtagga ggtgccgtcg atcacgatgc gcttcagcag
     4501 ctggccgccg tcccacttcc acgaccacgt ggtgaaggtc gggaacatcc ggacctgctg
     4561 gttggcggtg cccgcagtgt tggtggcccg ttcgacgcgg atgccgtcgg agagcgcggc
     4621 cggcgacgcg ccgttgatga ggccggagat ggtggcgtcg gcgagggcgg cgggggcgaa
     4681 cgcgctcggt ccggacacgt tggtgcgcac cgcggcggtc ccgccctggc cgttgggaga
     4741 ccagttccag ccctggcggc cccggccgat caggacccag ccgccgccgt cggtcttcat
     4801 gtcgcagtag aactgcgccg gtcggtccat cgcgggggtc gacagccagt aggtgccgct
     4861 ggggctggtg ggaaacgccc gcaggatgcc gaagcacgag gccccggcgg tggcagccga
     4921 ggcaccgtcg cccggccccg ggcgcggggg c
//
LOCUS       DATMFS010000398         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_200164, whole genome shotgun sequence.
ACCESSION   DATMFS010000398 DATMFS010000000
VERSION     DATMFS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200164"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VNQ33_07415"
     CDS             complement(<1..972)
                     /locus_tag="VNQ33_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ97972.1"
                     /translation="MHRTPPPLRIVVVGNGMVGHRLLTALAELGATPGAAITVIAGEP
                     RPAYDRVALSSWFAGRSAEDLSLVEPGFFADHGIEVLLGDPAAAIDRGSATVTTASGR
                     VLAYDRLVLATGSRPFVPPVPGHDGPGCFTYRTIGDLEAIATAASRPAVERGVVVGGG
                     LLGLEAANALLQLGLETHVVEFAPRLMPVQLDEGGSAALRRRVEHLGVHVHTSCATQA
                     IEHERGQPARLAFDGRDPLPADLVVFSAGIRPDDALARAAGLAVGERGGIAVDDQMRT
                     SDPAVLAIGECAVVGGRHFGLVAPGYQMAKVAAAHLAAQVLGTPEPTD"
     gene            1177..2259
                     /locus_tag="VNQ33_07420"
     CDS             1177..2259
                     /locus_tag="VNQ33_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HWJ97973.1"
                     /translation="MRTSKHLAAALLAGALVLSACSSDDGGAKNTTTEAPVPVDVGEV
                     LQGLAQDVIVPSYVGLSAALDKLAVDSKALCVAPSQEALTKAQDDWRAADVAWQKTRA
                     AGVGPALDDRLMSDVGFAARQTVVDDLLESGDPVDPASIAEEGSAARGIFASEILLFG
                     DGSDTLTTADALQRCEYAASVAANAKEAAGAVTKQWVDGDAKQRFVAGLDGGPESSVA
                     LVVNEVTHRLDELDTMALRDMAAAKSADDLDPSRKGGPADQRLADRRALFDGVSVTIG
                     NGRTGISALVGAQDAGTRDRLLAANDKANQAMAALPDSVSAAYEDPAAIKAASDAVAE
                     LKVLVSTEVASKLGVTITFSDSDGDS"
     gene            complement(2285..3319)
                     /locus_tag="VNQ33_07425"
     CDS             complement(2285..3319)
                     /locus_tag="VNQ33_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWJ97974.1"
                     /translation="MTRHLRIAAVALFATALVLTSCAESDGGAGTASNSKVLHVYSGR
                     HYDVEKAFKEYEEETGVKIEFLTGVDAELRERIAAEGADTEADAYITVDAGNLASAAK
                     AGLFRPIDSPKLEKVIPAQYRDPDGRWFGLALRARTIIYNTDALSEDEVPKHYEDLAK
                     PEWKGRVCLRNSTNDYQQSLVASMIIQDGEAETLKVLEGWTRNAEIYANDTEMIQAMG
                     AGACDVGIANHYYLARELEQNPDLPVGLIWANQDTGGTHVNISGGGVTKYAKNPKLAQ
                     DFLEWLGTKGQSVLVDSNHEFPASTAATPEPLITKEFGLDFKRQPLDAKKIGELNPEA
                     VRLMDQAGFG"
     gene            complement(3378..4430)
                     /locus_tag="VNQ33_07430"
     CDS             complement(3378..4430)
                     /locus_tag="VNQ33_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ97975.1"
                     /translation="MTTTPPPLQVHGAVRRFGSTRAVDGIDLELRAGELLVLVGPSGC
                     GKSTLLRAIAGLVALDEGRIEVGGETVDDGRSATPPEHRPVGMVFQDHSLFPHLTIGD
                     NVAFGIRKKPAAERKAHVAELLEMVEIDHLIGRYPHEVSGGERQRASVARAVAPHPRL
                     LLLDEPFAALDPNLREQVRTQLVTLLRRTGTPAVFVSHDQAEAMALGDRVAVMRAGRL
                     EQIGTGPDIYDRPANRFVASFMGDAAFLPVTTGGDGSATELGPIDHPADPADGPLLDA
                     VIRPADVDLDADPDGASEIIDSVYQGPTWTYGVRLPSGAVVRSTRPRSLALDHGDRVT
                     ASLDTKSPPLVPRTGP"
     gene            complement(4427..>4761)
                     /locus_tag="VNQ33_07435"
     CDS             complement(4427..>4761)
                     /locus_tag="VNQ33_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ97976.1"
                     /translation="SGLDQVPVDLTSSARSLGSRPATVLRRIHLPLSKGSMLTAAILV
                     GVDALKELPIALLLRPIGFDTLPVWTYNLASESRFQQAALPALTIIAAALIPVALLSR
                     GLDRRSSE"
BASE COUNT          660 a         1630 c         1812 g          659 t
ORIGIN      
        1 gtccgtcggc tcgggggtgc cgagcacctg ggcggccagg tgggccgcgg cgaccttggc
       61 catctggtaa cccggagcga cgaggccgaa gtgccggccg ccgaccacgg cgcactcgcc
      121 gatcgcgagg acggccgggt ccgaggtgcg catctggtcg tcgacggcga tgccgccgcg
      181 ctcgccgacg gcgagccccg cggcgcgggc gagggcgtcg tcgggccgga tgccggccga
      241 gaacaccacg aggtcggccg gcagggggtc gcggccgtcg aacgccaggc gggcgggctg
      301 gccgcgctcg tgctcgatcg cctgggtggc gcacgacgtg tggacgtgga cgccgaggtg
      361 ctcgacgcgc cgccgcagcg cggcgctccc gccctcgtcg agctgcaccg gcatgaggcg
      421 gggggcgaac tcgaccacgt gggtctccaa cccgagctgg agcagtgcgt tggccgcctc
      481 gaggccgagg aggccgccgc cgaccacgac gccccgctcc accgccggcc gggatgcggc
      541 ggtggcgatg gcctcgaggt cgccgatcgt ccggtacgtg aagcagcccg ggccgtcgtg
      601 gcccggcacg ggcggcacga acgggcgcga gccggtggcg agcaccagcc ggtcgtaggc
      661 caagacccgg cccgatgccg tcgtgaccgt ggcggaaccc cggtcgatgg cggcggccgg
      721 atcgcctagc agcacctcga tgccgtggtc ggcgaagaag cccggctcca cgaggctgag
      781 gtcttcggcg ctgcggccgg cgaaccacga cgagagcgcg acgcggtcgt aggcggggcg
      841 gggctcgccg gcgatcacgg tgatggcggc tcccggcgtg gcgccgagct cggccagggc
      901 ggtgaggagt cggtgcccga ccatgccgtt gccgaccacg acgatgcgga gcggaggagg
      961 cgtgcggtgc atgggaccaa ccaaccggaa ggcggttgcc ccgccgtttc cctcccgtga
     1021 accgaccggg tcgcacagct gggctgacgt ggacctgacc tgtgcgccgc cgcacaggtc
     1081 cgggccggac gtgtgcggtc cgtggcgcgg tcgtgaaccg gtcgggcgcg cgcccggtga
     1141 cggccagggc ggggagcggc gttagggtcc cttgacatgc gcacgtcgaa gcacctcgcc
     1201 gccgccctgc tggccggcgc gctcgtcctc tccgcctgct cgtccgacga cgggggcgcc
     1261 aagaacacga cgaccgaggc accggtaccc gtcgatgtcg gcgaggtcct ccagggactg
     1321 gcccaggacg tgatcgtgcc gtcgtacgtc ggtctgagcg cggcgctgga caagctggcc
     1381 gtcgactcga aggcgctgtg cgtggcgccg tcccaggagg cgctgaccaa ggcgcaggac
     1441 gactggaggg cggcggacgt ggcctggcag aagacgcgcg ccgccggcgt cggccccgcg
     1501 ctcgatgacc ggctgatgag cgacgtcggg ttcgccgccc gccagaccgt cgtcgacgac
     1561 ctgctcgaga gcggcgatcc ggtcgacccc gcatcgatcg cggaggaggg ctcggccgct
     1621 cgtggcatct tcgccagcga gatcctcctg ttcggcgacg ggtcggacac gctgaccacc
     1681 gccgacgccc tccaacgctg cgagtacgcc gcgtccgtgg ccgccaacgc caaggaggcg
     1741 gccggcgccg tcacgaagca gtgggtcgac ggcgatgcca agcagcggtt cgtcgccggg
     1801 ctcgatggcg gcccggagtc ctccgtggcc ctcgtggtca acgaggtgac gcaccgcctc
     1861 gacgagctcg acacgatggc cctgcgcgac atggccgcgg cgaagagcgc ggacgacctc
     1921 gacccctccc ggaagggcgg accggccgac cagcgcctgg ctgatcgccg agcgctgttc
     1981 gacggcgtct ccgtcaccat cggcaacggc aggacgggca tctcggcgtt ggtcggtgcg
     2041 caagatgccg ggacccggga ccgcctgctg gctgcgaacg acaaggcgaa ccaggccatg
     2101 gccgcccttc ccgactccgt gtcggcggcc tacgaggatc cggcggccat caaggcggca
     2161 tcggatgccg tcgccgagct gaaggtgctg gtcagcaccg aggtcgccag caagctcggc
     2221 gtcaccatca ccttcagcga ctccgacggc gactcctgaa cgagccgctc cctccgacga
     2281 cgggtcagcc gaagccggcc tggtccatca ggcgcaccgc ctcggggttc agctccccga
     2341 tcttcttggc atcgagcggc tggcgcttga agtcgaggcc gaactccttg gtgatgaggg
     2401 gctcgggcgt agcggcggtg ctggccggga actcgtggtt gctgtcgacg agcacgctct
     2461 ggcccttcgt gcccagccac tcgaggaagt cctgggccag cttcgggttc ttggcgtact
     2521 tggtgacgcc cccacccgag atgttgacgt gcgtgccacc cgtgtcctgg ttggcccaga
     2581 tcaagccgac cggcagatcc gggttctgct cgagctcgcg agccaggtag tagtggttgg
     2641 cgatgccgac gtcgcaggcc ccggccccca tggcctggat catctcggtg tcgttcgcgt
     2701 agatctcggc gttgcgggtc cagccctcga gcaccttcag cgtctccgcc tcgccgtctt
     2761 ggatgatcat gctggcgacg agggactgct ggtagtcgtt ggtggagttg cgcaggcaga
     2821 cccgcccctt ccactccggc ttggccaggt cctcgtagtg cttcggcacc tcgtcctcgc
     2881 tgagggcgtc ggtgttgtag atgatggtgc gggcccggag ggccaggccg aaccaccggc
     2941 cgtcggggtc tcggtactgg gccgggatca ccttctcgag cttcggcgag tcgatcggcc
     3001 ggaacagccc ggccttcgcc gcgctggcga ggttgccggc gtcgaccgtg atgtaggcgt
     3061 cggcctcggt gtcggcgccc tcggccgcga tgcgctcgcg cagctcggcg tcgaccccgg
     3121 tgaggaactc gatcttcacg ccggtctcct cctcgtactc cttgaaggcc ttctcgacgt
     3181 cgtagtggcg cccggagtac acgtgcagga ccttgctgtt cgacgcggtg ccggccccgc
     3241 cgtcggactc ggcgcagctg gtcaggacca gagcggtggc gaacagggcg acggcggcga
     3301 tgcgcaggtg acgggtcacg acggtcctct cggcgggggc gtgccgacgt tagccacgcc
     3361 taacacccgc ccaacagtca gggaccggtg cggggcacca gcggcgggct cttcgtgtcc
     3421 aaggacgcgg tcacgcggtc gccgtggtcg agcgcgagcg agcggggccg cgtggagcgg
     3481 acgacggcac ccgacggcaa ccggacgccg taggtccagg tgggcccctg gtagacggag
     3541 tcgatgatct cgctggcgcc gtcgggatcg gcgtcgaggt cgacgtcggc cggacggatc
     3601 acggcatcga gcagcgggcc gtccgctggg tccgccgggt ggtcgatcgg gccgagctcg
     3661 gtcgcggacc cgtcgcctcc cgtggtgacg ggcaagaagg cagcatcgcc catgaacgac
     3721 gccacgaagc ggttcgccgg acggtcgtag atgtccggtc cggtcccgat ctgctccaag
     3781 cgcccggccc gcatgacggc gacccggtcg cccagcgcca tggcctcggc ctggtcgtgc
     3841 gacacgaaca ccgccggggt gccggtccgg cgcagcaggg tgacgagctg ggtgcgcacc
     3901 tgctcgcgga ggttcgggtc gagcgcggcg aacggctcgt cgagcagcag caggcggggg
     3961 tggggcgcca cggcgcgggc gaccgacgcc cgctggcgct cgccgccgga cacctcgtgg
     4021 gggtagcggc cgatcaggtg gtcgatctcc accatctcga gcagctcggc gacgtgggcc
     4081 ttgcgctcgg cggccggctt cttgcggatg ccgaaggcca cgttgtcgcc gatggtcagg
     4141 tgggggaaga gcgagtggtc ctggaagacc atgcccacgg gccggtgctc gggcggcgtc
     4201 gccgatcgac cgtcgtccac cgtctcgcca cccacctcga tgcgcccctc gtcgagggcg
     4261 acgagcccgg cgatggcgcg caggagggtc gacttgccgc atccgctggg accgacgagc
     4321 accagcagct cgccggcgcg gagctcgaga tcgatgccgt cgacggcacg ggtggagccg
     4381 aaccggcgga cggcgccgtg cacctgcagc gggggagggg tcgtggtcac tcggaggacc
     4441 tccggtccag gcccctcgag agcagggcca cagggatgag tgcggcggcg atgatcgtga
     4501 gcgccggcag ggccgcctgc tggaagcgcg actccgacgc caggttgtag gtccacaccg
     4561 gcagcgtgtc gaagccgatc ggtcgcagga gcagcgcgat gggcagctcc ttcagggcgt
     4621 cgacgccgac gaggatggcg gcggtgagca tgctgccctt cgagagcggc aggtggatgc
     4681 ggcgcagcac cgtggccggc cgcgagccca gcgaccgggc cgacgaggtg aggtcgaccg
     4741 gcacctggtc cagaccggac t
//
LOCUS       DATMFS010000399         7054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_153570, whole genome shotgun sequence.
ACCESSION   DATMFS010000399 DATMFS010000000
VERSION     DATMFS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153570"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VNQ33_07440"
     CDS             <1..230
                     /locus_tag="VNQ33_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97977.1"
                     /translation="RMTFRMKGSVYPKDRMVFTATVTDVTTDDAGCGWASLLVTLAVD
                     GVTKTDCSVRVALPVSADDNPWSRRGGQWIP"
     gene            289..1437
                     /locus_tag="VNQ33_07445"
     CDS             289..1437
                     /locus_tag="VNQ33_07445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ97978.1"
                     /translation="MDLDFSEEQDMLREMVRGVCSTSSSLDAVRALEDDPIGYSPEFW
                     QQLADLDLTGLMIPEAYGGSGMTALEGAVVYEELGRALAPSPHFVSAVLSAGLLLRSG
                     SEDQKQALLPQIAKGTAIATTAWLEPKNGFGPKGVQATATADGDGFVLAGTKWHVPFA
                     AAATHLVVLARTGPAPTDVDLFVVDPKAGGVTLTQMFSIASDCQYQVELDGVRVTEAD
                     RIGAAGTGWATWDAVLDEGLVFLAAQAIGGAAYALEITVQYAKDREQFDKPLGAFQAI
                     SHYLSDASTAVNGGRILVYEAAWSLANGRPVEGKDVSTLGPMAKLFACQTYRNTTAMG
                     EQVFGGVGFTLEYDIQLFFRRAKALQIAWLDDPALREKVATQVLDKTA"
     gene            1676..2623
                     /gene="gluQRS"
                     /locus_tag="VNQ33_07450"
     CDS             1676..2623
                     /gene="gluQRS"
                     /locus_tag="VNQ33_07450"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWJ97979.1"
                     /translation="MATGRFAPSPTGDLHLGNLRTALVAWLFARQAGSGFLVRMEDLD
                     RVQASADHERSQLADLAALGLDWDGEVVRQSERFDRYEAAIARLTAAGFTYPCSCTRR
                     EIREASAAPHGDPLPDGAYPGTCRDLTDAERQAQVESGRPLALRLRGPGTTVTVHDEL
                     QGEVSALVDDVVLRRNDGVPAYNLAVVVDDAAQGIEQVVRGDDLASSAPRQRYLADLL
                     GLAPVAYAHVPLVLGPTGQRLAKRDGAVTVADQLALGRTPEQLVGTLAHSLGLVDAPE
                     PVRAADLIAAFEPTRITSAPWTFETTHLHCHPHPGSNFS"
     gene            2925..3314
                     /locus_tag="VNQ33_07455"
     CDS             2925..3314
                     /locus_tag="VNQ33_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWJ97980.1"
                     /translation="MGETHIEGPTAVVLIAHGSRTVSANKAHRKMAVDLETMLGMPVW
                     AAFLELADPLIPAAIHQATAAGATKVLVLPYFLYPGRHLSLDIPELVATTAAMHPDVD
                     IVTMDGFGEDPAVLGLLVAQVRRADAR"
     gene            3329..4198
                     /locus_tag="VNQ33_07460"
     CDS             3329..4198
                     /locus_tag="VNQ33_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWJ97981.1"
                     /translation="MAYERVAHDPRFRSGPPAVPNKTLPAVPEPLAALLTGVPAEQVW
                     VKRESEVWRVQLGGVPAYLKIMDDLGPFADELARLRWCTDHSPIATPHLLGDALDDLG
                     RGWFVASEVAGTPAHDAALAEHDPHGVVRSIAGGLRRLHDETDPGTCPFALGVDDLIA
                     SAEARVAIGGVDPTSMRDPAYQRQTAAQLLDHLIATRPPEPIEDQVVTHGDPCQPNLL
                     LAAAADGLELAGLVDLGRLAVSDRYRDLAIAHRSLDMNLGPDAAAELLATCGPTDVDH
                     DRLTWWTLVDDLW"
     gene            complement(4335..5204)
                     /locus_tag="VNQ33_07465"
     CDS             complement(4335..5204)
                     /locus_tag="VNQ33_07465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWJ97982.1"
                     /translation="MAAPPLRSRRWFIALVALVVAGLAVGGIVWGQSRDGGSQQVTAS
                     LSLPGARASKVAGTWPTTTTTTTAPSTLDPATGLSISGPIGPPWPDHFTAADAIVSNV
                     ALYEAPGNPVPTGRTLPNPTAEGFKLVFLVRKQKGNWLQVQINQRPNGATAWVKSSDV
                     AVRTVPNHILIELGAKKLTVFHGEEPIFTASVAPGKASSPTPTGAFYTDILTRPSNPN
                     GPYGPYQISVTGFSNVYESFGGGNGQIAIHGTNRPELIGQPASHGCVRMNNEDISALI
                     PLARQGTPVVIVD"
     gene            complement(5257..5685)
                     /locus_tag="VNQ33_07470"
     CDS             complement(5257..5685)
                     /locus_tag="VNQ33_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97983.1"
                     /translation="MATPIELVGYWASEDDLEWPDPGDFVDPSWDVHERSLVAGYLER
                     GRTFRAAGGWSTCRLCDRRNGSSEKQDGMYYWPEGLAHYVTDHAVRLPDRFVRHVLAT
                     MASSHPDEPSNAETMGPSDIELAEDWWQRQTSYSPEAEPT"
     gene            complement(5715..6065)
                     /locus_tag="VNQ33_07475"
     CDS             complement(5715..6065)
                     /locus_tag="VNQ33_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97984.1"
                     /translation="MLKNLRAKLSQSIDDIEGERLQGRLADLGIQPIADAPTRTPVLL
                     AGEVQGLQVVPRAGSPSLEVTFSDGSGRAVAVFTGRKRLGGVDCGRRIVVDGVGRTER
                     GRMVVLNPAYTIVE"
     gene            6173..6355
                     /locus_tag="VNQ33_07480"
     CDS             6173..6355
                     /locus_tag="VNQ33_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HWJ97985.1"
                     /translation="MGFLDKAKDALQGKSDEAAKALDKAGDVIDDKTGGKYTDKIDQA
                     VDKASDVLDDIDKQND"
     gene            complement(6413..>7054)
                     /locus_tag="VNQ33_07485"
     CDS             complement(6413..>7054)
                     /locus_tag="VNQ33_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWJ97986.1"
                     /translation="SVGKFIGEQLQAAGHEVTILDNDKAVIAQARRSGEPAGVSWYGG
                     DACELDTLAAAGVGQADVVAAVTGDDEDNLVISLLAKQEFAVPRVVARVNNPKNEWMF
                     NDMWGVDVSVSTPHLLTALVEEAVSVGSLVRLLSFEGGRARLSEATLAPGSPANGKDL
                     AELGLPRDSTVVAIIRDDHVIVPRGDTVLRGGDEVLVLTTPDAEDDVVSTLLG"
BASE COUNT         1027 a         2543 c         2440 g         1044 t
ORIGIN      
        1 gccgcatgac gttccgcatg aagggctccg tgtacccgaa ggaccgcatg gtgttcaccg
       61 ccaccgtcac cgacgtgacc accgacgacg ccggctgcgg ctgggcctcg ctgctcgtca
      121 ccttggccgt cgacggcgtg accaagaccg actgctcggt gcgcgtggcc ctgcccgtgt
      181 ccgccgacga caacccctgg tcccgccgcg gcggccagtg gatcccctga ccgcacgcat
      241 ccgcccggcg cactgcaccc gacgcaccga acaccgagga ctgaacccat ggacctggat
      301 ttctccgaag aacaagacat gctccgagag atggtgcgcg gcgtgtgcag cacgtcgtcg
      361 tcgctcgatg cggtgcgcgc cctcgaggac gaccccatcg ggtactcgcc cgagttctgg
      421 cagcagctgg ccgacctcga cctcaccggc ctgatgatcc ccgaggcgta cggcggttcc
      481 ggcatgaccg cgctcgaagg tgcggtcgtc tacgaggagc tgggccgggc cctggccccg
      541 tcgccccact tcgtgagcgc cgtgctgagc gccgggctcc tgctgcggtc cgggtccgag
      601 gaccagaagc aggccctgct cccgcagatc gccaagggca ccgccatcgc caccacggcg
      661 tggctcgagc cgaagaacgg attcggcccc aagggcgtgc aggccacggc cacggccgac
      721 ggtgacggct tcgtcctcgc cggcaccaag tggcacgtgc ccttcgccgc cgccgcgacg
      781 cacctcgtgg tgctcgcccg caccggcccg gcaccgaccg acgtcgacct gttcgtcgtg
      841 gacccgaagg ccggcggcgt caccctcacc cagatgttct cgatcgcctc ggactgccag
      901 taccaggtgg agctcgacgg cgtccgggtc accgaggccg accgcatcgg cgccgccggc
      961 accggctggg ccacctggga cgccgtgctc gacgagggcc tcgtgttcct cgccgcccaa
     1021 gccatcggcg gtgccgccta cgccctggag atcacggtcc agtacgccaa ggaccgcgag
     1081 cagttcgaca aaccgctcgg cgccttccag gccatctccc actacctgtc cgacgcctcg
     1141 acggcggtca acggcggccg catcctggtc tacgaggcgg cgtggtcgct ggccaacggc
     1201 cgcccggtcg agggcaagga cgtgtccacg ctcggcccca tggcgaagct gttcgcctgc
     1261 cagacctacc gcaacaccac ggcgatgggc gagcaggtct tcggcggcgt cggcttcacc
     1321 ctcgagtacg acatccagct cttcttccgc cgggccaagg ccctccagat cgcctggctc
     1381 gacgaccccg ccctccgcga gaaggtcgcc acccaggtcc tcgacaagac cgcctgaccc
     1441 cccgcatccc cgggccgcac atccaccgac ccccgcgatt tcgggtcacg cgtgcaccgt
     1501 caacgtgccc aggtgacccg aaatcggtgg aatcgggatt cctcactgat ttcgggtcac
     1561 gcgtgcacgg cctgggtgcc caggtgacct gaaatcgact ggcgccggct cgccgtgtgg
     1621 gacccgtcga ccgcgggcgc ttcgccggga gggggtccgc tgccatgctg gaccgatggc
     1681 caccggtcgg ttcgctcctt cgccgacagg ggacctgcac ctggggaacc tgcggacggc
     1741 gctcgtggcg tggctgttcg cccggcaggc gggatcgggg ttcctcgtgc ggatggagga
     1801 cctggaccgg gtgcaggcat cggccgacca cgagcggtcg cagctggccg acctggccgc
     1861 gctcgggctc gactgggacg gcgaggtcgt ccgccagagc gagcggttcg accgctacga
     1921 ggcggccatc gcccggctca ccgccgccgg gttcacctac ccctgctcgt gcacccgacg
     1981 ggagatccgc gaggcgtcgg cggccccgca cggcgatccg ctgcccgacg gcgcctaccc
     2041 cgggacctgc cgggacctga ccgacgccga gcggcaggcc caggtcgagt cgggtcgccc
     2101 gctcgcgctg cggctgcgtg gcccgggcac gaccgtgacc gtccacgacg agctgcaggg
     2161 tgaggtgtcg gcgctcgtcg atgacgtggt cctccgccgc aacgacggcg tgcccgccta
     2221 caacctcgcc gtcgtggtgg acgacgccgc ccagggcatc gagcaggtgg tgcgcggcga
     2281 cgacctggcc agctcggcgc cccgccagcg ctacctcgcc gacctgctcg gtctcgcgcc
     2341 cgtggcctac gcccacgtcc cgctcgtcct cggtcccacc ggccagcgcc tggccaagcg
     2401 cgacggcgcc gtcaccgttg ccgaccagct cgccctcggt cgtacgcccg agcagctcgt
     2461 cgggaccctc gcccactcgc tcggcctcgt cgacgctccc gagccggtgc gagccgccga
     2521 cctcatcgcc gcgttcgaac cgacccgcat cacctcggca ccgtggacgt tcgagacgac
     2581 gcatctccac tgtcaccctc accctggatc gaacttttcg tgaagttggt tgccctgcca
     2641 ccacgatcac gaaaagtttg ggtgggttgg ggtggggatg ggtcagatgc cttgggcgat
     2701 gcggaggcgg acgatctccc tgatcgcaag cggctcgacg cttccgtccc tgtctggaga
     2761 cccgcttcag gttcgtggat tcgcgtcacc tgctgcgtgg ctcatcgccc caggatcacg
     2821 agcggtgggt cgacctgctc gatgcgacca gcctgttcgt gcctcgagcg gagccctgtg
     2881 tcgagggtcc accgcaacac gggatcgccg gtacggtccc acccatgggt gagacccaca
     2941 tcgagggacc gaccgccgtc gtgttgatcg cgcacgggag ccggacggtg tcggccaaca
     3001 aggcgcaccg gaagatggcc gtcgacctgg agacgatgct gggcatgccg gtgtgggcgg
     3061 cgttcctgga gctggccgac ccgctgatcc ccgcggcgat ccaccaggcc actgcggccg
     3121 gcgccaccaa ggtgctggtc ctgccgtact tcctctaccc gggccgccac ctgtcgctcg
     3181 acatccccga gctggtggcc accaccgcgg ccatgcaccc tgacgtggac atcgtgacga
     3241 tggacggctt cggcgaggat ccggcggtcc tcggtctgct cgtcgcccag gtccgccggg
     3301 ccgacgcccg ctgacccggg gcgacgccat ggcgtacgag cgggtcgccc acgacccccg
     3361 gttccgatcg ggcccgccgg ccgtgccgaa caagacgctc cctgcggtgc ccgagccgct
     3421 ggccgcgctg ctcaccgggg tgcccgccga gcaggtctgg gtgaagcggg agtccgaggt
     3481 gtggcgcgtg cagctcggcg gcgtgccggc ctatctcaag atcatggacg acctcggacc
     3541 gttcgctgac gagctcgccc ggctccgctg gtgcaccgac cacagcccga tcgccacccc
     3601 gcacctgctg ggcgatgctc tcgatgacct gggccgcggc tggttcgtcg cctcggaggt
     3661 ggccggcacg cccgcacacg acgccgccct cgccgagcac gatccgcacg gcgtggtccg
     3721 ttcgatcgcc ggtgggctcc gccgcctcca cgatgagacc gacccgggga cgtgtccctt
     3781 cgccctgggc gtcgatgacc tcatcgccag cgcagaggcc cgcgtggcga tcggcggcgt
     3841 cgaccccacg tcgatgcgcg acccggcgta ccagcgccag accgccgccc aactcctcga
     3901 ccacctgatc gcgacccgtc cgcccgagcc gatcgaggac caggtcgtca cccacggtga
     3961 tccctgccag cccaacctcc tgctcgcagc ggcggccgac ggcctcgagc tggcgggctt
     4021 ggtcgatctc ggccgcctcg ccgtctccga ccgctaccgg gacctggcca tcgcccatcg
     4081 ctcgctcgac atgaacctcg gcccggatgc cgccgccgag cttctcgcca cctgcggccc
     4141 gaccgacgtc gaccacgacc gcctcacctg gtggaccctt gtcgacgatc tctggtgacg
     4201 cccaccggaa tcgggtcacg cgtgcaccgc atcggtgctc aggtgacctg attcagcgct
     4261 cgtcgtggga atggaaccca atcgggtcac gcgtgcaccg gatcggtgct caggtgacct
     4321 gattgtggcg tcggtcagtc gacgatgacg acgggggtgc cctggcgggc gagcgggatc
     4381 agcgcgctga tgtcctcgtt gttcatgcgg acgcagccgt ggctcgcggg ttggccgatc
     4441 agctccggcc ggttggtgcc gtggatggcg atctggccgt tgccgccgcc gaagctctcg
     4501 tagacgttcg agaacccggt gacgctgatc tggtagggcc cgtacggccc gttgggattg
     4561 ctgggccggg tcaggatgtc ggtgtagaac gcgccggtgg gcgtgggcga cgacgccttg
     4621 ccgggggcga ccgacgcggt gaagatcggc tcctcaccgt ggaacacggt cagcttcttg
     4681 gcgccgagct cgatgaggat gtggttgggg accgtgcgga cggcgacgtc ggacgacttg
     4741 acccaggccg tggcgccgtt cggccgctgg ttgatctgga cctggagcca gttgcccttc
     4801 tgcttccgca ccaagaagac gagcttgaag ccctcggccg tggggttggg cagggtgcga
     4861 ccggtgggga cgggattgcc gggcgcttcg tagagggcca cgttcgagac gatggcgtcg
     4921 gcggcggtga agtggtcggg ccagggcggc ccgatgggac ccgagatgct cagccccgtg
     4981 gccgggtcca gcgtcgacgg cgccgtggtc gtggtcgtcg tggtcggcca cgtcccggcc
     5041 accttcgagg cccgggcacc cggcagtgac agcgaggcag tgacctgctg cgagccgcca
     5101 tcacgtgact gcccccagac gatgcctccc accgcgagcc cggcgaccac cagcgccacc
     5161 agcgcaatga accagcgtcg cgagcggagg ggaggggcgg ccatagagcg tcaacgctac
     5221 cgatctggat gtccgtccgg tagggcggcg gaggggtcag gtcggctccg cctctggcga
     5281 gtagctcgtc tgcctctgcc accagtcctc ggcgagctcg atgtctgatg gccccatcgt
     5341 ctcggcgttg ctcggctcat ccgggtggct cgacgccatg gtcgccagca cgtgtcggac
     5401 gaaccggtca gggaggcgca ccgcgtggtc cgtcacgtag tgagcaaggc cctcgggcca
     5461 gtagtacatc ccatcctgct tctcggagct tccgttgcgc cgatcgcaca ggcggcaggt
     5521 cgaccagcca cctgccgccc ggaaggtccg cccccgttcc agatagccag cgacgaggga
     5581 gcgctcgtgc acatcccagg agggatcgac gaagtcacca gggtcaggcc actcaaggtc
     5641 gtcctcgctg gcccagtacc cgaccagttc gatgggcgtc gccatggcgt cagtggggcc
     5701 gagagccgaa gcgcctactc gacgatggtg taggcggggt tgagcacgac catgcggccc
     5761 cgctcggtgc ggccgacgcc gtcgaccacg atgcggcggc cgcagtcgac gccgcccagg
     5821 cgcttgcggc cggtgaacac ggcgaccgcc cgcccgctgc cgtcgctgaa ggtgacctcg
     5881 agcgacgggg aaccggcgcg gggcaccacc tggaggccct ggacctcgcc ggcgagcagg
     5941 acgggggtcc gagtgggcgc gtccgcgatg ggctggatcc cgaggtcggc gaggcgcccc
     6001 tggagccgct cgccctcgat gtcgtcgatg ctctgcgaga gcttggcgcg gaggttcttc
     6061 agcacggcgc ccactgtcgc acggtggtgc ctgcactggc aacgggcgca gcgatcatgt
     6121 cttgacgcct accgtggtcc cacccccacg acccacacgc aaggagcacg acatgggatt
     6181 cttggacaag gcgaaggacg cgctgcaggg caagagcgac gaagcggcga aggcgctcga
     6241 caaggccggc gacgtcatcg acgacaagac cggcggaaag tacaccgaca agatcgacca
     6301 ggcggtcgac aaggcgtccg acgtcctcga cgacatcgac aagcagaacg actagaagac
     6361 cagccgtcgc tcgcccgctg cccatcgggg cgggcgatgc gtcggcggtc ggctacccca
     6421 ggagggtcga gacgacgtcg tcttcggcgt cgggggtggt gagcaccagg acctcgtcgc
     6481 cgccgcgcag gacggtgtcg ccacggggga cgatcacgtg gtcgtcgcgg atgatggcca
     6541 ccacggtgga gtctcggggg aggcccagct cggcgaggtc cttgccgttg gcaggcgagc
     6601 cgggggcgag ggtggcctcg gacaggcggg cccggccacc ctcgaacgag agcaggcgga
     6661 cgaggctgcc caccgacacg gcctcttcga cgagcgcggt gagcaggtgc ggggtcgaga
     6721 ccgagacatc gacgccccac atgtcgttga acatccactc gttcttcggg ttgttgaccc
     6781 gggccaccac gcgggggacg gcgaactcct gcttggcgag cagggagatc acgaggttgt
     6841 cctcgtcgtc gccggtgacg gcggccacga cgtcggcctg gccgacaccg gcggcggcga
     6901 gcgtgtcgag ctcgcaggcg tcgccgccgt accagctgac acccgccggc tcgcccgagc
     6961 gacgggcctg ggcgatgacg gccttgtcgt tgtcgaggat ggtcacctcg tgcccggccg
     7021 cctgcagttg ctcgccgatg aacttgccga cgct
//
LOCUS       DATMFS010000400         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_293668, whole genome shotgun sequence.
ACCESSION   DATMFS010000400 DATMFS010000000
VERSION     DATMFS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293668"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VNQ33_07490"
     CDS             <1..847
                     /locus_tag="VNQ33_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ97987.1"
                     /translation="GLLRMAAAHQLTVLAGVPTMWNAMLHADVEVSRDDLAGLTHALS
                     GGAALPLAVADAFRERFGCRVLDGYGLSETTGAGTSAKLSPRRKEGSVGPALHRLEAS
                     VVDSEGNPVPFGERGEVALAGPVIMKGYWRRPEATAEVMRGKWFLTGDIGKQDEDGDI
                     WILDRKKDLVIRGGYNVYPREIEEVLYTHPDLREVAVIGVPDDRLGEEVAAVYAPHPG
                     RVVESKDLRAWLEERLAQYKVPRIYHEVAELPKGSTGKILKRQLDRGSVLEHGMKVTR
                     DRSAS"
     gene            844..1866
                     /locus_tag="VNQ33_07495"
     CDS             844..1866
                     /locus_tag="VNQ33_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWJ97988.1"
                     /translation="MTQLIETGPIAAWIETLGIDAVGPIEFTRVGAGQSNLTFLVTDA
                     ADHRWILRRPPLGTLLASAHDVEREHRIMSGLQGTGVPVPTIHGFTKDPAVTDAPIML
                     MDFVDGRVLDTIEAVESVPEETRRAIGLSLASTLGEVHAVDLEKAGLVDLASHKPYAE
                     RQLKRWHRQWEQSRTRDLPIVDDLAARLSANVPQHQDLALVHGDFHLMNVITDPSAGR
                     VTAVLDWELSTLGDPLADLGGLLAYWPQADDEIITGFRGPTMPGFPSRAELVEEYARA
                     TGRDVSAIGFWYVLGLWKIAIIAEGVLRRSIDDPRNTAVTGQISSELIDELMQRADLE
                     ARDAGL"
     gene            1970..>2584
                     /locus_tag="VNQ33_07500"
     CDS             1970..>2584
                     /locus_tag="VNQ33_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ97989.1"
                     /translation="MKLNYRGGSGRPSALRRGMGSAAVVALAASLLAACGGGSDSSAG
                     TSGEAPDQATLVLRNDVDTFDPMLTAAEQGAVQMYEALYDTLVRRNWKTGEYEPAMAT
                     KWEATATKIDFELKTGLKCSDGTDLKPSDIANSLKRLADPKTGSIYTGRFFGAGGVKE
                     IVGDDAANTVSVEVNEPHSDLIDSMATAFIVCPKGLEDTEKLATE"
BASE COUNT          416 a          926 c          898 g          344 t
ORIGIN      
        1 gggcctgctg cggatggccg cggcgcacca gctcaccgtg ctggccggcg tgccgacgat
       61 gtggaacgcg atgctccacg ccgacgtcga ggtctcgcgc gacgacctcg ccggactgac
      121 ccacgccctc tcgggcggcg ccgccctgcc gctcgccgtc gcggacgcct tccgcgagcg
      181 cttcggctgc cgggtcctcg acggctacgg cctcagcgag accaccggcg ccggcacctc
      241 ggcgaagctc agcccgcgcc gcaaggaggg ctcggtcggc cccgcgctgc accgcctcga
      301 ggcgagcgtc gtggactccg agggcaaccc cgtgccgttc ggcgagcgcg gcgaggtcgc
      361 cctggccggg cccgtcatca tgaagggcta ctggcgtcgt cccgaggcca ccgccgaggt
      421 catgcgcggc aagtggttcc tcaccggaga catcggcaag caggacgagg acggcgacat
      481 ctggatcctc gaccgcaaga aggacctcgt catccgtggc ggctacaacg tctacccgcg
      541 cgagatcgag gaggtgctct acacgcaccc cgacctgcgc gaggtcgccg tgatcggcgt
      601 ccccgacgac cgcctcggcg aggaggtcgc cgccgtctac gccccgcacc cgggccgtgt
      661 cgtcgagagc aaggacctgc gcgcctggct ggaggagcgg ctggcccagt acaaggtccc
      721 gcggatctac cacgaggtgg ccgagctgcc caagggctcg accggcaaga tcctcaagcg
      781 ccagctggac cgcggcagcg tgctcgagca cggcatgaag gtgacgcggg acaggagcgc
      841 ctcgtgacgc agctgatcga gaccggaccg atcgcggcct ggatcgagac cctcggcatc
      901 gacgccgtgg gcccgatcga gttcacccgc gtgggcgccg gtcagtcgaa cctcacgttc
      961 ctggtcaccg acgccgccga ccaccgctgg atcctgcgcc gcccgccgct gggcacgctg
     1021 ctggcctcgg cccacgacgt cgagcgcgag caccgcatca tgtccggcct gcagggcacc
     1081 ggagtgccgg tgccgacgat ccacgggttc acgaaggacc cggcggtcac cgacgcgccg
     1141 atcatgctca tggacttcgt cgacgggcgc gtgctcgaca cgatcgaggc cgtggagtcg
     1201 gtgcccgagg agacccgccg ggcgatcggc ctctcgctgg cctcgacgct gggcgaggtg
     1261 cacgccgtgg acctcgagaa ggcggggctg gtcgacctgg ccagccacaa gccgtacgcc
     1321 gagcgccagc tcaagcgctg gcaccgtcag tgggagcagt cgcgcacccg cgacctgccg
     1381 atcgtggacg acctcgcggc gcggctgagc gcgaacgtcc cgcagcacca ggacctggcc
     1441 ctcgtgcacg gcgacttcca cctcatgaac gtcatcaccg atccctcggc cggccgcgtc
     1501 accgcggtgc tggactggga gctgtcgacc ctcggcgacc ccctggccga cctcggcgga
     1561 ctgctggcct actggccgca ggccgacgac gagatcatca ccgggttccg cgggccgacg
     1621 atgcccgggt tcccgagccg cgccgagctc gtggaggagt acgcccgcgc gaccggacgc
     1681 gacgtctcgg cgatcgggtt ctggtacgtg ctgggcctgt ggaagatcgc gatcatcgcc
     1741 gagggcgtgc tgcgccgctc gatcgacgac ccccggaaca ccgcggtgac gggccagatc
     1801 tcgagcgagc tcatcgacga gctgatgcag cgggccgacc tcgaggcgcg tgacgcaggt
     1861 ctgtgaccca gggcaccccc tgtaggtaaa gttgaaatca agttcatata gactcgtggc
     1921 gagtgccgcg acccccgccg gcacgtagtg agtctgtgag aggtcgagca tgaagttgaa
     1981 ctaccgagga ggctccggtc gtccgtccgc gctgcgtcgc ggcatgggat cggcggccgt
     2041 cgtggccctg gccgcgtcgc tgctggcggc atgcggcgga ggctcggact cgtctgccgg
     2101 cacgtccggc gaggcgcccg accaggccac gctggtgctc cgcaacgacg tggacacctt
     2161 cgatccgatg ctgacggccg ccgagcaggg cgccgtgcag atgtacgagg cgctctacga
     2221 cacgctcgtg cgccgcaact ggaagaccgg cgagtacgag ccggcgatgg ccacgaagtg
     2281 ggaggccacg gccaccaaga tcgacttcga gctcaagacg ggcctgaagt gcagcgacgg
     2341 cacggacctc aagccgagcg acatcgccaa ctccctcaag cgcctcgccg atcccaagac
     2401 cggctcgatc tacaccggcc gcttcttcgg cgccggcggc gtgaaggaga tcgtcggcga
     2461 cgatgccgcg aacaccgtct cggtcgaggt caacgagccg cactcggacc tgatcgacag
     2521 catggccacc gcgttcatcg tgtgcccgaa gggcctcgag gacaccgaga agctggccac
     2581 cgag
//
LOCUS       DATMFS010000401         4112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_340395, whole genome shotgun sequence.
ACCESSION   DATMFS010000401 DATMFS010000000
VERSION     DATMFS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340395"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VNQ33_07505"
     CDS             complement(<1..1067)
                     /locus_tag="VNQ33_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97990.1"
                     /translation="MTSDEAFTGFVADAEPRLRRAFTLLRGGDVGADATAEALAWAWE
                     HWAEVQAMGNPVGYLYRVGSSKTRYREERFPHAVPPPDAPGFEPRLVPALARLTLRQR
                     TAVVLVHGCGWTHQEVADALADDELAPAVETLAPRRRRWLAAAAAVFLVALVGTGLAV
                     RASDDGEHLRAGESVTTTMPSTEPVDVSFQQLGASNDSAAAGALAAAQNDEELRQLRI
                     DAGVSDRSEVDFSTHVVVAFTVVGRDCPATFSVDPPMLGGMVRTGTDLEPQFLIPDAC
                     DANDTPRTFVVAVPWAETGDEFDLVLSGNLGGEAGRQLHVTREHHEPAPPSKVTASLQ
                     LDATTVPVGGTITGTVTVVND"
     gene            1242..1436
                     /locus_tag="VNQ33_07510"
     CDS             1242..1436
                     /locus_tag="VNQ33_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97991.1"
                     /translation="MQSTSVRIDVKTHEQLKELAAELGTTVGHTVTLAVRLFRQDLMG
                     QQLSRPLDEDEIAWLDADLG"
     gene            1420..1764
                     /locus_tag="VNQ33_07515"
     CDS             1420..1764
                     /locus_tag="VNQ33_07515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWJ97992.1"
                     /translation="MLTSGDVVDLDLGIPHGSEAGFRHPAVVVTAQASLRRGAAVVQI
                     VPTTSTIRGEHSEVVIEPDRLNGLDRPSAAQCQHIRGVSSARIADVRGNVGPVVLAQV
                     RDRLAVLLGLPV"
     gene            complement(1769..2230)
                     /locus_tag="VNQ33_07520"
     CDS             complement(1769..2230)
                     /locus_tag="VNQ33_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97993.1"
                     /translation="MAYVPALRFPRSAEERADPALAWRRSDEAFFASGACHILAFTFR
                     DQHPDRDLAIRFIDPADGFVGTHLYVVDGGWALDFNGWTPEAELLAVTGAAWADAYSG
                     WSYELRDIPAAVDLEAFCADGLRRAPRFYAHDPRPRARAYLARFPDRPPVR"
     gene            complement(2262..3620)
                     /locus_tag="VNQ33_07525"
     CDS             complement(2262..3620)
                     /locus_tag="VNQ33_07525"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWJ97994.1"
                     /translation="MTELAKADFGDDFTWGVAHASYQVEGAWNVGGKGPSIWDTFTHG
                     GGRIRNKATGDTACDFYHRYESDTDLVKTLGFDAQRLSISWPRVLPNGTGRVNGAGLD
                     FYDRVVDACLERGVAPWVTLYHWDLPQALQDQGGWADRRIVGWFEEYVGVVADRLGDR
                     VKDWMVFNEPCSFLVVGHLLGVHAPGTRSLRKYLAGTHHVNLAQAVGAEVLRAKVPDA
                     NVGTTHIVTPMRSKRDTERDRQAKASIDVIANRIFWEPNFGLGYPIDDSPIIAGVERY
                     IRPGDDRAVVVDWDFIGVQYYQRQLVKPWPVPKLRGVPWISRDFRNYEITAMGWEVQP
                     DGLYEAIERFHAYGKVPRLYVTENGSAYPDVLTGGRVHDDRRTAFYEAHLAEVKRAVV
                     DGLPVHGYFCWSLMDNFEWAEGYDPRFGIVHVDFATQQRTIKDSGYWFQQFLGAESTV
                     SS"
     gene            complement(3652..>4112)
                     /locus_tag="VNQ33_07530"
     CDS             complement(3652..>4112)
                     /locus_tag="VNQ33_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWJ97995.1"
                     /translation="GGSVPTEANETEHAADMIGQGKVEASPFAVALASASVASGRSLE
                     PRLIIDPDEPEPKRGEQLDPTAIEALRGLMRGVVTDGTGSALLGIPGGDVAGKTGTAE
                     FGRENPPQTHAWFTGYQGDIAFAVLVEDGGFGGQVAAPLAAKFLTKLAEG"
BASE COUNT          591 a         1473 c         1452 g          596 t
ORIGIN      
        1 ctgtcgttga ccacggtgac cgttcccgtg atggtcccgc cgacgggcac ggtcgtcgcg
       61 tcgagctgga gcgatgccgt gaccttcgac ggaggagccg gttcgtggtg ctccctcgtc
      121 acgtggagct gccggccggc ctcgccaccg aggttgcccg acagcaccag gtcgaactcg
      181 tcgccggtct ccgcccacgg gacggcgacc acgaaggtcc gaggcgtgtc gttcgcgtcg
      241 caggcgtccg ggatgaggaa ctgcggttcg aggtcagttc ccgtccggac catcccgccc
      301 agcatcggcg ggtcgacgct gaaggtggcc gggcagtccc gtcccaccac ggtgaaggcg
      361 accaccacgt gcgtcgagaa gtcgacctcg gaccggtcgc tcacgccggc atcgatcctc
      421 agctgtcgga gctcctcgtc gttctgcgcc gcagcgagcg ctccggctgc cgcgctgtcg
      481 ttgctcgcgc ccagctgctg gaacgagacg tcgaccggct cggtggacgg catcgtcgtg
      541 gtcaccgact cacccgcccg caggtgctcg ccatcgtcgc tcgcccgcac ggccagaccg
      601 gtgcccacga gcgccaccag gaacacggcg gcagccgcgg ccagccaacg gcggcggcgc
      661 ggcgccaggg tctcgacggc gggcgcgagc tcgtcgtcgg ccagcgcgtc ggccacctcc
      721 tggtgcgtcc acccgcagcc gtgcaccagc acgaccgccg tgcgctggcg cagcgtcagc
      781 cgggcgagcg ccggcaccag cctcggctcg aacccggggg catcaggcgg cggcacggcg
      841 tgcgggaagc gctcctcgcg gtaccgggtc ttgctgctcc cgacccggta caggtacccg
      901 accgggttgc ccatcgcctg cacctcggcc cagtgctccc acgcccacgc cagcgcctcg
      961 gcggtcgcat cggccccgac gtccccgccc cgcagcaacg tgaacgcccg ccgcagccgg
     1021 ggctcggcgt cggccacgaa cccggtgaac gcctcgtcac tcgtcaccgt cgtcgcctcc
     1081 acaccaacaa gagcacaccg agagcggttt gtacgacgcg atgcgccaga tggatcgcat
     1141 ctcgtctcga tggcggaggg atcgggcacg ctgccgacat gagcgctgca ccgttgagat
     1201 cgtcagacgc cgccaccacc accggttgta tgctgatctg catgcagtcg accagcgtcc
     1261 gcatcgacgt gaagacgcac gaacagctga aggagctcgc cgccgagctc ggcaccaccg
     1321 tgggccacac cgtcacgctt gccgtgcgtc tcttccgcca ggacctcatg ggtcaacagc
     1381 tgtcccgccc gttggacgag gacgagatcg cctggctcga tgctgacctc gggtgatgtc
     1441 gtcgatctcg acctcggaat cccgcacggc agcgaggccg ggttccgcca ccccgccgtg
     1501 gtggtcaccg ctcaggcgag cctgagacga ggagccgccg tggtgcagat cgtcccgacc
     1561 acgtcgacca tccgcggtga gcactccgag gtcgtgatcg agcccgaccg cctcaacggg
     1621 ctcgaccgtc catcggcggc acagtgccag cacatccgcg gcgtctcgtc cgcgaggatc
     1681 gcggatgtcc gcggcaacgt cggccccgtc gtgctcgccc aggtgcgcga tcgactcgcg
     1741 gtcctcctcg gccttcccgt ctgaccggtc accgaaccgg cgggcggtcg gggaagcggg
     1801 cgaggtaggc ccgggctcgg gggcgggggt cgtgggcgta gaaccggggg gctcggcgga
     1861 ggccgtcggc gcagaaggcc tcgaggtcga cggcggccgg gatgtcccga agctcgtacg
     1921 accagccgga gtaggcatcg gcccacgctg ctccggtcac cgccagaagc tcggcctcag
     1981 gcgtccagcc gttgaagtcg agcgcccagc cgccgtcgac cacgtagagg tgggtgccga
     2041 cgaacccgtc cgccgggtcg atgaaccgga tcgccagatc ccggtcgggg tgctggtccc
     2101 ggaacgtgaa ggccaggatg tggcacgcgc ccgaggcgaa gaacgcctcg tcggaccgcc
     2161 gccacgccag cgccggatcg gcccgctcct ccgccgagcg agggaaccgg agcgccggca
     2221 cgtaagccat cagatcgccc agccgccgct ctgccggagg ctcacgacga gacggtggac
     2281 tcggcgccga ggaactgctg gaaccagtag ccggagtcct tgatggtgcg ctgctgagtg
     2341 gcgaagtcga cgtggacgat gccgaagcgc gggtcgtagc cctcggccca ctcgaagttg
     2401 tccatcagcg accagcagaa gtagccgtgc accgggaggc cgtcgacgac cgcccgcttc
     2461 acctcggcga ggtgggcctc gtagaacgcg gtgcggcggt cgtcgtgcac gcgcccgccg
     2521 gtgagcacgt cggggtaggc cgacccgttc tcggtgacgt acaggcgcgg caccttgccg
     2581 taggcgtgga agcgctcgat cgcctcgtag agcccgtcgg gctggacctc ccagcccatc
     2641 gccgtgatct cgtagttccg gaagtcccgg ctgatccacg gcacgccgcg cagcttcggc
     2701 accggccacg gcttcaccag ctggcgttgg tagtactgca cgccgatgaa gtcccagtcg
     2761 acgacgaccg cccggtcgtc gccgggtcgg atgtaccgct cgacgccggc gatgatcggc
     2821 gagtcgtcga tcgggtagcc gaggccgaag ttcggctccc agaagatgcg gttggcgatg
     2881 acgtcgatgc tcgccttggc ctggcggtcg cgctcggtgt cgcgcttcga gcgcatcggc
     2941 gtgacgatgt gggtggtgcc gacgttcgca tcgggcacct tcgcccgcag cacctcggcg
     3001 cccacggcct gggccaggtt cacgtggtgc gtgccggcca ggtacttgcg cagcgagcgg
     3061 gtgccggggg cgtggacccc gagcaggtgg ccgaccacca ggaagctgca cggctcgttg
     3121 aagaccatcc agtccttcac ccggtcgccc aggcggtcgg cgacgacgcc gacgtactcc
     3181 tcgaaccagc cgacgatgcg ccggtcggcc caaccgccct ggtcctggag ggcctgcggg
     3241 aggtcccagt ggtacagggt cacccagggg gcgacgccgc gctcgaggca ggcgtcgacc
     3301 acgcggtcgt agaagtcgag gccggccccg ttgacccggc cggtgccgtt cggcagcacc
     3361 cgcggccacg agatcgagag ccgctgggcg tcgaagccga gggtcttgac caggtccgtg
     3421 tcggactcgt accggtggta gaagtcgcac gcggtgtcgc cggtggcctt gttgcggatc
     3481 cggccgccgc cgtgcgtgaa cgtgtcccag atcgacgggc ccttgccgcc gacgttccac
     3541 gccccctcca cctggtagct ggcatgggcc accccccacg tgaagtcgtc cccgaagtca
     3601 gccttcgcca gttccgtcat ggccgccaag gtacgcggat cggacgagcg gtcagccttc
     3661 cgccagcttg gtcaggaact tcgcggcgag gggggcggcg acctggccgc cgaagccgcc
     3721 gtcttccacc aggacggcga aggcgatgtc gccttggtag ccggtgaacc aggcgtgggt
     3781 ctgcggcggg ttctcccggc cgaactcggc ggtgccggtc ttgccggcca cgtcgccacc
     3841 ggggatgccg agcagggcac tgccggtgcc gtcggtgacg acgccgcgca tcagaccgcg
     3901 gagcgcctcg atggcggtgg ggtcgagctg ctcgccccgc ttcggctccg gttcgtcggg
     3961 gtcgatgatc agccgcggct cgaggctgcg gccgctggcc accgacgccg aggcgagcgc
     4021 caccgcgaac gggctggctt cgaccttgcc ctggccgatc atgtcggcgg cgtgctcggt
     4081 ctcgttggcc tcggtgggca ccgagccgcc gg
//
LOCUS       DATMFS010000402         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_60146, whole genome shotgun sequence.
ACCESSION   DATMFS010000402 DATMFS010000000
VERSION     DATMFS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60146"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VNQ33_07535"
     CDS             complement(<1..566)
                     /locus_tag="VNQ33_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ97996.1"
                     /translation="MPNHLVRRSLAAAIVSLSVAAVAGCGATGGAGGDGAAQIVSSGV
                     APEAQQASFLAHAAQTTGAVKTEKVAVTVRTTPADGDPSVRVTSTGEIDGAGGRAHVT
                     ADVSGTVSGWSDEAAVEAVYDGDTVYVKAPFTQVLGGEPWVKATSPLLAKLGEGLGDG
                     LQGDPGSFLELLEGAGGPVTTVGTTDVRG"
     gene            725..1678
                     /locus_tag="VNQ33_07540"
     CDS             725..1678
                     /locus_tag="VNQ33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWJ97997.1"
                     /translation="MIARLVISALLLWVLIAKIGDSWHDAAPEPKAATFAWLAGALLL
                     TFGAVVLSAVRWSAVLQALGLHPPFNRLLSLYFAGQFMGNVLPSTIGGDALRVSRLSK
                     DNGESPTTFASVVLERMSGWVVLPVLTLFALLINPGLRELGRASMIAFVTAAATLTLL
                     VVVLVLTSRQGKGVEGRLEHNDGWRRFTSAVRLGIRKLSHEPAATGRILVSGLAYQLI
                     LIGSALMAAKALDLPTAVGPTALLAFVPAVLIAQVLPISISGLGVREGLFVLFLHPLG
                     VPQSQAIALGLLMYLLNLVVSLFGAPAFAVGHRVRPASPAP"
     gene            1675..2544
                     /locus_tag="VNQ33_07545"
     CDS             1675..2544
                     /locus_tag="VNQ33_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWJ97998.1"
                     /translation="MTATTTAAPVDTPRSEGLKWWHEVLIGGAFYLVYSQIRNLFGAG
                     PESRTIAFGHARDVIEIERTLHLWIEPHIQRWYLGLPGHGFIKVWNVYYGTAHFVITV
                     GVLVAAYRLAPHRYRFIRTTLAGTTALALIGFASFTLMPPRLLGVDSPYGACRGLGPD
                     CHGYGIVDTIDRWGGLWKFGEGGMAAVSNQYAAMPSLHIGWSMWCALSMIIVIGRGRR
                     RYWWFLYPATTFFCIIVTGNHYWLDAFFGLVALAGGTLTALGFEKLRTRRLARQAGRS
                     DEGAATETAPATA"
     gene            complement(2550..>2877)
                     /locus_tag="VNQ33_07550"
     CDS             complement(2550..>2877)
                     /locus_tag="VNQ33_07550"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HWJ97999.1"
                     /translation="RAPAPEWLREQLAIAQRGCTTVVAHSIMFQYLSDDGRREMLAAI
                     DAAGRAATPDAPFAWLRLEPGGDQAELRLTAWPGGTTHLLAKSSYHGPPVVWLAPEGG
                     VLKAPR"
BASE COUNT          368 a         1100 c          959 g          450 t
ORIGIN      
        1 ccgcgcacgt cggtggtgcc gacggtggtg acgggtccgc ccgcaccctc caggagctcg
       61 aggaagctgc cggggtcgcc ctggaggccg tctcccagcc cctcgcccag cttcgccagc
      121 aggggcgagg tggccttcac ccacggttcc ccgccgagga cctgggtgaa cggcgccttc
      181 acgtagacgg tgtcgccgtc gtacacggcc tcgacggcgg cctcgtcgga ccagccggag
      241 acggtgcccg acacgtcggc ggtgacgtgg gcgcggccgc cggctccgtc gatctcgccg
      301 gtggacgtga cgcgcacgga cgggtcgccg tcggccggcg tggtgcgcac ggtgacggcc
      361 accttctcgg tcttcaccgc gcccgtcgtc tgggccgcat gagcgaggaa cgacgcctgc
      421 tgcgcctcgg gcgccacgcc cgacgacacg atctgggccg ctccatcgcc accggccccg
      481 cccgtggcac cgcaaccggc gacggccgcg acgctgagcg acacgatggc tgcggcgagc
      541 gatcgtcgga cgagatggtt cggcatcagc acccctgcag cgccgtcccc ctcaccgtga
      601 gggtcgtccg gcggcgaagc gcaacgatac cgggaccctg ctccagaagg gcagccgggt
      661 agggtcgagc ggcaacttgg acggacgggg ctggctcgtg caactcacga aaccgcggct
      721 gatcatgatc gcacgcctcg tgatcagcgc cctcctgctg tgggtgctca tcgccaagat
      781 cggcgacagc tggcacgacg ccgcacccga gccgaaggcg gccacgttcg cctggctcgc
      841 cggcgccctc ctcctcacct tcggcgccgt cgtcttgtcc gccgtccggt ggagcgcggt
      901 gctgcaggcc ctcggcctcc acccgccgtt caaccgcctc ctgtcgctct acttcgccgg
      961 gcagttcatg ggcaacgtgc tgcccagcac gatcggaggc gatgccctgc gggtctcgcg
     1021 cctgtcgaag gacaacggcg agtcccccac cacgttcgcc tcggtcgtgc tcgagcgcat
     1081 gagcggctgg gtcgtgctcc ccgtcctcac cttgttcgcc ctgctcatca accccgggct
     1141 ccgagagctc gggcgggcgt cgatgatcgc cttcgtcacc gccgcggcga ccctgaccct
     1201 gctggtcgtc gtgctcgtcc tcacctcgcg ccagggcaag ggcgtcgagg ggcggctgga
     1261 gcacaacgac gggtggcggc gcttcaccag cgccgtgcgc ctcggcatcc gcaagctgtc
     1321 gcacgaaccg gccgccaccg ggcgcatcct cgtcagcggc ctggcctacc agctcatcct
     1381 catcgggtcc gcgctgatgg cggccaaggc gctggacctc cccaccgcgg tcgggcccac
     1441 cgcgctgctc gcgttcgtgc ccgctgtgct catcgcccag gtgctcccga tctccatctc
     1501 cggcctgggc gtgcgcgagg ggctgttcgt gttgttcctg caccccctcg gcgtgccaca
     1561 gtctcaggcg atcgccctgg gcctgttgat gtacctgctc aacctcgtgg tgagcctctt
     1621 cggtgccccg gccttcgccg tcggccaccg ggtccggccg gcctcccccg caccatgacc
     1681 gcaaccacca ccgccgcccc cgtcgacacc ccccggtccg agggtctcaa gtggtggcac
     1741 gaggtcctga tcggcggcgc gttctacctc gtctactcgc agatccggaa cctgttcggg
     1801 gccggtcctg agtcgcggac gatcgcgttc ggccacgccc gagacgtcat cgagatcgag
     1861 cggacgctcc acctgtggat cgaaccgcac atccagcggt ggtacctcgg tcttcccggc
     1921 cacgggttca tcaaggtctg gaacgtgtac tacggcacgg cgcacttcgt catcaccgtg
     1981 ggcgtcctgg tcgcggccta ccggttggcg ccgcaccgct accggttcat ccgcaccacg
     2041 ctcgccggca ccacggcgct cgccctcatc ggcttcgcct cgttcacgct gatgccgccc
     2101 cgcctgctgg gtgtggactc gccctacggg gcctgccgcg gcctgggccc cgactgccac
     2161 ggctacggca tcgtcgacac catcgaccgg tggggcggtc tctggaagtt cggcgagggc
     2221 gggatggccg ccgtctcgaa ccagtacgcc gccatgccga gcctccacat cggctggtcg
     2281 atgtggtgcg ccctcagcat gatcatcgtg atcggccggg gccggaggcg gtactggtgg
     2341 ttcctgtacc cggccaccac gttcttctgc atcatcgtca ccgggaacca ctactggctc
     2401 gacgccttct tcggactggt ggccctggcc ggcggcacgc tcaccgccct cggcttcgag
     2461 aagctgcgaa cgaggcgcct cgcccgccag gccggccggt ccgacgaggg cgccgccacc
     2521 gagaccgccc ccgccaccgc ctgactcggc tacctcggcg ccttgagcac cccgccctcg
     2581 ggcgcgagcc acacgaccgg tggcccgtgg tacgaggact tggccagcag gtgggtcgta
     2641 ccgccgggcc aggccgtgag ccggagctcg gcttgatcgc cgcccggttc gaggcgcagc
     2701 caggcgaacg gcgcatcggg cgtagccgcc cgaccggccg cgtcgatggc ggcgagcatc
     2761 tcgcgccggc cgtcatcgga caggtactgg aacatgatcg agtgggcgac cacggtcgtg
     2821 cacccgcgct gggcgatggc gagctgctcc cgcagccact ccggcgccgg ggcccgg
//
LOCUS       DATMFS010000403         5432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_340466, whole genome shotgun sequence.
ACCESSION   DATMFS010000403 DATMFS010000000
VERSION     DATMFS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340466"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VNQ33_07555"
     CDS             <1..429
                     /locus_tag="VNQ33_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98000.1"
                     /translation="LALGVRFGLRGASLLGVGAAAGAVGAATATWRAVPPDAGASVIP
                     VLVAAVALVAALAGALGPARVRLVAVAATAASLLGWGVLRWSVLARAVLPTTLDPWLD
                     RAATAVAIGVGLGLAGLLVKGPSPRRRADPDPTVTVTPTA"
     gene            531..1049
                     /gene="flgN"
                     /locus_tag="VNQ33_07560"
     CDS             531..1049
                     /gene="flgN"
                     /locus_tag="VNQ33_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HWJ98001.1"
                     /translation="MEAVGTILEREGDALEVLLFKLIETNLLIEAGEARFLPRATREV
                     ERARNRARELDLLRATTVARTRSTATLRELAATAPGPWPAILRDHHDVLSRLVSEIEV
                     VAHLNARLAREGLDLLAHDPVRATVGAGAPASGRPIRNAELDRLARGAALDAVLGTAA
                     RLRMPDLIEFLR"
     gene            complement(1220..2062)
                     /locus_tag="VNQ33_07565"
     CDS             complement(1220..2062)
                     /locus_tag="VNQ33_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWJ98002.1"
                     /translation="MMIETDRETQRLIEDHLPLVRHVVFQVAVNFPRHVDREELATAG
                     ALGLVEAARRYDETRGVPFDRFAAQRIRGAILDAVRAADWAPRSVRNLARRLESIEQR
                     LASNLGRMPSADETAEALGVSKDELRRLQDKMFRSVVLALEYDVNDGDEDLTLVEVLT
                     DDATLEPSEELESRELKAYLRDAVHLLPERHRLVVVGYFLEGRKSQELASFLGVTESR
                     ISQLRSEALEMLREGITAQYEADNGVPTEAPQGRVARRKAGYASAIGEASHWHDRIGE
                     VATA"
     gene            complement(2267..3244)
                     /locus_tag="VNQ33_07570"
     CDS             complement(2267..3244)
                     /locus_tag="VNQ33_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ98003.1"
                     /translation="MHVAVVGLGAVGTRAARQLASTDAVERVTLYETQDGRVGEVSSS
                     LGRKAHVGAGTPEALADVDVVVLATPCGTHLAPARALVGRGVPVVSTSDDVDEVRGLL
                     DLGPEAEARGTAVAVGAAFSPGLTCLLARLAADRLDRVDEIHVARLGTGGPACARQHH
                     GSLGGVGLDWRDGGWQRRAAGSGRELSWFPDPIGAADCYRAALPDPLLLVPAFPGVGR
                     VTARLAGTRRDRLTARLPMLRPPHPEGGLGAIRVEVRGRGPAGISVEILGAMDRPGVA
                     GGAVAALSAVELGAGRARHAGASGLATLVEVRPFLRELARRGVKVAEFG"
     gene            complement(3244..3381)
                     /locus_tag="VNQ33_07575"
     CDS             complement(3244..3381)
                     /locus_tag="VNQ33_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98004.1"
                     /translation="MTMIRRALVALGLAGLVAAVLRVRGSGGTPPQRGGWRELSPDEL
                     R"
     gene            complement(3410..3483)
                     /locus_tag="VNQ33_07580"
     tRNA            complement(3410..3483)
                     /locus_tag="VNQ33_07580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3447..3449),aa:Ile,seq:gat)
     gene            complement(3550..4044)
                     /locus_tag="VNQ33_07585"
     CDS             complement(3550..4044)
                     /locus_tag="VNQ33_07585"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HWJ98005.1"
                     /translation="MTAPAAALAVAERKPATDPPTAPTPSVAAPAVPSAPPAATGRPV
                     RRGRRVKRVLRRIDLWSVLKLSLVVYTCLYIAVLATLAAIWGILYQSGNVDKLQSFLG
                     DVGLENFTFYGNQMFRACAAIGAVGVLAGTVITVLATALVNIISEMTGGIRLIVIEED
                     VDPR"
     gene            complement(4232..4573)
                     /locus_tag="VNQ33_07590"
     CDS             complement(4232..4573)
                     /locus_tag="VNQ33_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98006.1"
                     /translation="MTDRRRTTTTPRPTERGQAAPLMLVALLFAALVGVGIVRVGAAA
                     GVAASAQAAADAAALAAAADGEDAAAEVAAANEAEVVTYESIGEDVRVTIERHGARAT
                     ARARWQPAPIP"
     gene            complement(4566..>5432)
                     /locus_tag="VNQ33_07595"
     CDS             complement(4566..>5432)
                     /locus_tag="VNQ33_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HWJ98007.1"
                     /translation="KAQGRGGRGISGAKLKDEDYIAHIVTTTAHAYLLFFSNLGRVYR
                     LKAHEIPMKDRTARGTAIVNLLPLQPDEKIQTIIDTRDYETNQYLFFATRNGQVKKTR
                     FSEYDSSLRAGLIAINLRDGDELVKVMPVNDADEIFMVSRSGMTIRYAQDDVRPMGRS
                     TAGVRGMKMKPGDQVVSCDVARDDTAILIVTDGGYGKRTQLDKFNIQGRGGQGVRGIK
                     LTAKKGFVVAAFMVAIDDEILAIASDGVVTRMEVREISSQGRDATGVKVMNVGGDRTV
                     AAVAPVLSADDD"
BASE COUNT          736 a         1967 c         1976 g          753 t
ORIGIN      
        1 ctagccctgg gcgtccggtt cgggctcagg ggtgcgtcgt tgctgggcgt cggcgccgcc
       61 gcaggcgccg tcggcgccgc caccgccacc tggcgcgccg tcccgcccga cgccggggcg
      121 tcggtcatcc ccgtgctcgt cgccgccgtc gccctggtgg ccgccctcgc cggtgccctc
      181 ggtcccgccc gggtccgcct cgtggccgtc gccgcgaccg cggcctcgct cctcggctgg
      241 ggagtcctgc ggtggagcgt cctcgcccga gccgtcctgc cgacgaccct cgatccgtgg
      301 ctcgaccgcg ccgcgaccgc ggtggccatc ggcgtcgggc tcgggttggc tggcctcctg
      361 gtgaaggggc cgtcgcctcg gcgacgcgcc gacccggacc cgaccgtcac cgtcaccccg
      421 accgcttgaa ccgccgcccg agggggcacc cggcccacca gcggcctcgc ggagcgagaa
      481 aaccagggta tggcttcacc ctgcgtggtc gaggccgatg gatggcgggc atggaagccg
      541 tcggaacgat cctggagcga gagggcgatg cgctcgaggt cctgctcttt aagctcatcg
      601 agaccaacct gctgatcgag gccggtgaag cccgcttcct ccctcgcgcc acccgggagg
      661 tcgaacgggc ccgcaaccgg gctcgggagc tcgacctgct ccgcgccacg accgtggcgc
      721 gcacccgttc caccgccacg ctgcgggagc tcgccgcgac cgcgccgggg ccctggccgg
      781 cgatcctccg ggaccaccac gacgtgctca gccgcctcgt ctcggagatc gaggtcgtcg
      841 ctcacctgaa cgcccggctc gcccgcgagg gactcgacct gctggctcac gaccccgtgc
      901 gggccaccgt cggggcgggc gcaccggcca gcggccgccc gatccgcaac gccgagctcg
      961 accggttggc gagaggcgcc gccctcgacg ccgtcttggg caccgcggcc cgcctccgca
     1021 tgcccgacct catcgagttc ctgcggtagc gctcggcccg gtcgcctgcc ccttcgaaac
     1081 ctcgggctcg aacgccgctc ggaggcaggg accggcgcgg caggaccgcg acccggaggc
     1141 cgctgtcctg tgaacgcccg ccgtcgagat ggagggcggg ggtggtgatg ggcgtcgcgc
     1201 gccgggcgac gcaccgcgat caggcggtgg cgacctcacc gatgcggtcg tgccagtgcg
     1261 acgcctcgcc gatcgccgag gcgtagccgg ccttgcggcg ggccacccgg ccctgggggg
     1321 cttcggtggg cacgccgttg tccgcttcgt actgggcggt gatgccctcg cggagcatct
     1381 cgagcgcctc cgagcggagc tgggagatcc gggactcggt gacgccgagg aacgaggcca
     1441 gctcctggga cttccggccc tccaagaagt agccgacgac cacgaggcgg tgccgctccg
     1501 ggaggaggtg cacggcgtcg cgcaggtacg ccttgagctc acggctctcg agctcttcgg
     1561 agggctcgag cgtggcgtcg tcggtgagga cctcgaccag cgtcaggtcc tcgtcgccgt
     1621 cgttgacgtc gtactccaag gcgaggacca ccgaacggaa catcttgtcc tggaggcggc
     1681 gcagctcgtc cttgctgacc ccgagcgcct cggcggtctc gtcggccgac ggcatgcggc
     1741 cgaggttgct ggcgagccgc tgctcgatcg actcgagccg gcgggcgagg ttgcgcaccg
     1801 agcggggggc ccagtccgcg gcccggaccg catcgaggat cgcaccccgg atgcgctggg
     1861 cggcgaagcg gtcgaacgga acgccgcggg tctcgtcgta gcggcgggcc gcctcgacca
     1921 gaccgagtgc accagcggtg gccagctcct cccggtcgac gtggcggggg aagttcaccg
     1981 ccacctggaa gaccacgtgg cgcaccagcg gcaagtggtc ttcgatcagc ctctgggttt
     2041 cgcggtcggt ctcgatcatc atctcatcgc cctctttcgt gttgacccgt gtccagtggg
     2101 tgcagagagg gttacggacc gaagttgagg agacttgaac gatttcttgg caaaacgctc
     2161 gcgatcggtc ccgaatcggg acctttgagt cgttcggccc agttgggacc gagaaattgt
     2221 cgcacaccgt ggtcataacg ccacggatgg tggctgacct gcgggttcag ccgaactcgg
     2281 cgaccttgac cccgcgccgc gccagttcgc gcaggaacgg ccgcacctcg acgagggtgg
     2341 cgagacccga tgcgccggcg tggcgcgctc gccccgcacc gagctccacg gcgctgagcg
     2401 ccgccaccgc accgccggcg acacccggtc ggtccatggc ccccaggatc tccacggaga
     2461 tccccgccgg tccccgaccc cgcacctcga cgcggatggc gccgaggccg ccctcggggt
     2521 gcggtgggcg gagcatgggc aggcgggccg tgagccggtc ccggcgggtg ccggccaagc
     2581 gagcggtgac ccgcccgacg ccggggaacg ccggcaccaa gaggagcgga tcgggcagcg
     2641 cggcccgata gcagtcggcg gcgccgatcg gatcagggaa ccagctcagc tcccggcccg
     2701 acccggcggc ccggcgctgc cacccgccgt cacgccagtc cagcccgacg ccgccgagcg
     2761 acccgtggtg ctgacgggcg caggccggcc cacccgtccc caggcgggcg acgtggatct
     2821 cgtcgacgcg gtcgagccga tcagcagcca ggcgggcgag cagacaggtc aggccgggcg
     2881 agaaggcggc gccgacggcg accgccgtgc cccgtgcttc ggcctcgggt ccgaggtcga
     2941 gcaggcctcg cacctcgtcg acgtcgtccg acgtggagac cacgggaacg ccccggccca
     3001 ccagggcccg ggctggtgcc aggtgcgtgc cgcacggggt cgccagcacc acgacgtcga
     3061 cgtccgcgag cgcctcgggc gtacccgcac cgacgtgcgc cttgcggccc agcgacgacg
     3121 agacctcgcc gacccggccg tcctgcgtct cgtagagggt gacccgctcc acggcatcgg
     3181 tcgacgcgag ctggcgcgcc gcccgagtgc cgacggcgcc caggcccacc acggcgacgt
     3241 gcatcagcgc agctcgtcgg gcgacagctc tcgccagccc ccgcgctggg gcggcgtgcc
     3301 gccggacccc cggacccgca ggaccgccgc caccaggccg gccagcccca gggcgaccag
     3361 tgctcggcga atcatggtca cggctgcctc cgggatggga acgatctcgt ggggctagct
     3421 ggaatcgaac cagcgacctc acccttatca ggggtgcgct ctaaccaact gagctatagc
     3481 cccgagcggg agccgacggt agtgggtcgg ccccacagga tgcgaaccgc ttgtccggca
     3541 accggccggt cagcgggggt cgacgtcctc ttcgatgacg atcaagcgga tgccgccggt
     3601 catctcgctg atgatgttga ccagcgcggt ggccaacacg gtgatgaccg tgcccgccaa
     3661 gacgccgacg gcgccgatgg ccgcacaggc ccggaacatc tggttgccgt agaacgtgaa
     3721 gttctccagg ccgacgtcac cgaggaacga ctggagcttg tcgacgttgc cgctctggta
     3781 gaggatcccc cagatcgcgg ccagcgtggc cagcaccgcg atgtagaggc acgtgtagac
     3841 caccagcgag agcttgagga ccgaccacag gtcgatgcga cgcaggacgc gcttgactcg
     3901 gcgtccccgg cgcaccgggc ggccggtggc ggccggtggc gcgctgggca ccgcgggggc
     3961 tgcgacggac ggcgtcggcg ccgtgggcgg gtcggtggcg ggcttgcgct cggcgaccgc
     4021 gagcgccgcc gcgggtgccg tcaccgctcc gaagcgcgag ggcggggacg agaccgcgag
     4081 gccatcgccg cccttgcggt cggtaccgcc ggctggggca aggcgctcgg gtcggggtgg
     4141 ccgcttggga ccgctgcctg cccgcggggc gttgaccgac gccttcgctc cagcgggggg
     4201 cccaggaggc tcaacagaca cggcggccag cctaggggat gggcgcgggc tgccatcgag
     4261 cgcgggcggt ggcacgggcc ccgtgacgct cgatcgtcac gcgcacgtcc tcgccgatgg
     4321 actcgtaggt caccacctcg gcctcgttcg ccgccgccac ctcggcggcg gcgtcctcgc
     4381 cgtcggccgc ggcagcgagg gcggccgcat ccgcagccgc ctgagccgag gcggcgacgc
     4441 ccgccgcggc gccgacccgg acgatgccga cgccgacgag cgcggcgaac agcagcgcca
     4501 ccagcatcag cggcgcggcc tggcctcgct cggtcggacg gggggtggtg gtcgtccggc
     4561 gccggtcagt catcgtcggc ggagagcacc ggggccacgg ctgcgaccgt ccgatccccc
     4621 ccgacgttca tcaccttcac gcccgtagcg tcgcgaccct gcgacgagat ctcccggacc
     4681 tccatgcggg tcaccacgcc atcggaggcg atggccagga tctcgtcgtc gatggccacc
     4741 atgaaggcgg cgacgacgaa gcccttcttg gccgtgagct tgatgccgcg cacgccctgg
     4801 ccgccccggc cctggatgtt gaacttgtcg agctgggtgc gcttgccgta gccgccgtcg
     4861 gtcacgatga ggatcgcggt gtcgtcgcgg gcgacgtcgc acgagacgac ctggtcgccc
     4921 ggcttcatct tcatgccgcg gacgccggcg gtggagcggc ccatgggccg cacgtcgtct
     4981 tgggcgtagc ggatggtcat gccggatcgg ctgaccatga agatctcgtc ggcgtcgttc
     5041 accggcatga ccttcaccag ttcgtcgccg tcgcgcaggt tgatggcgat gaggccggcc
     5101 cgcagcgagg agtcgtactc gctgaagcgg gtcttcttca cctgcccgtt gcgcgtggcg
     5161 aagaacaggt actggttcgt ctcgtagtcc cgcgtgtcga tgatcgtctg gatcttctcg
     5221 tcgggttgga gcgggagcag gttgacgatg gccgtcccgc gagccgtgcg gtccttcatc
     5281 gggatctcgt gcgccttgag gcggtagacc cgcccgaggt tcgagaagaa cagcaggtac
     5341 gcgtgcgccg tggtggtcac gatgtgcgcg atgtagtcct cgtctttgag cttggcgccg
     5401 gagatgcccc ggccgccccg gccctgggcc tt
//
LOCUS       DATMFS010000404         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_109004, whole genome shotgun sequence.
ACCESSION   DATMFS010000404 DATMFS010000000
VERSION     DATMFS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109004"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VNQ33_07600"
     CDS             <1..558
                     /locus_tag="VNQ33_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98008.1"
                     /translation="GISMIRKMLIGAALLVVAAAAPAAAQTYDANVSPGTVVAGQEVT
                     VSGEGCVPGSTVSVKVTQRSTGTIVFTGSDTTDSTGEFTFKFKMPSDAAPGWYDLEAR
                     CPNGTGSSDRVAGVVEGNDLVWRMEILVVAPSTPTTTPTNGSSAPIVRTGSDLNGLGL
                     AGAGLITVGGIILIATRSRRHQARA"
     gene            664..1566
                     /locus_tag="VNQ33_07605"
     CDS             664..1566
                     /locus_tag="VNQ33_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWJ98009.1"
                     /translation="MDIAITGSTGLIGQALTTSLRAAGHHVIPVVRSASSPGTIRWDP
                     GAGTIDAASLEGVDAVVHLAGEGIASGRWTDAQRDRIRTSRERGTQVLATALAGLDAP
                     PSVLVSGSAIGYYGDRGDDVLAEGSGPGGDFLAGVCLAWEGATAPAEAAGIRTVHLRT
                     GIVLDAHGGALAKQLPAFKLGLGAKAGSGRQWMSWITLHDEVRAIRFALEEASLSGAL
                     NLTAPNPVTNASFTDAVGDALHRPTFLTIPAFVRKAPLGIGGLVESLLLVSQRCEPAA
                     LLTAGFTFDHEDLATALASVLGRR"
     gene            1620..2306
                     /locus_tag="VNQ33_07610"
     CDS             1620..2306
                     /locus_tag="VNQ33_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWJ98010.1"
                     /translation="MSGRRIAAFDFDGTLTRRDTLLPFLARACGATAFGRAVGHIAPQ
                     AVRARTGRLQAEVHHRDVTKAALLRELLAGRDASWYSAQGDQYATTLGPKLRPEMVEQ
                     VRWHRDAGHELVIVSASLRTYLDPFATSFGFDHVIAVDLEADDRGVLTGEMAGPNVRG
                     EEKAVRLRQWLGGEAPEMMWAYGNSSGDTELLAMADIAVWVAGRRRGQHANDRPAVGP
                     GRSFSDQQTA"
     gene            complement(2345..>2928)
                     /locus_tag="VNQ33_07615"
     CDS             complement(2345..>2928)
                     /locus_tag="VNQ33_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98011.1"
                     /translation="AAVEAAKDLGPLKALVNCAGIGWATRTIGKDGEYSSAHDLDIFK
                     KVVEINLIGTFDCIRIAATAMSQNEPDEFGERGAIVNTASVAAEDGQIGQASYSASKG
                     GVVGMTLPIARDLSAVGIRVNTILPGLIDTPIYGEGEASEQFKARLEVGVLFPKRLGQ
                     PAEFASLTAELLSNSYMNAESIRIDGGIRMQPK"
BASE COUNT          401 a         1061 c         1031 g          435 t
ORIGIN      
        1 ggaatctcga tgattcgcaa gatgctcatc ggcgcagcgc tgctcgtcgt tgccgcagcg
       61 gcccccgctg ctgctcagac gtacgacgcc aacgtgagcc cgggcaccgt ggttgctggt
      121 caagaggtga cggtgtcggg cgaaggctgc gtgccgggtt cgaccgtcag cgtgaaggtg
      181 acgcagcgct cgactggcac catcgtcttc accggctcgg acaccaccga ctcgacgggc
      241 gagttcacct tcaagttcaa gatgccgtcg gacgccgctc ccggctggta cgacctcgaa
      301 gcccgctgcc ccaacggtac cggttcctcc gatcgcgtcg ccggtgtcgt cgagggcaac
      361 gacctcgtct ggcggatgga gatcctcgtc gtcgctccct cgacgcccac cacgacgccc
      421 accaacggct cgtctgctcc gatcgtccgc actggttcgg acctcaacgg cctcggcctc
      481 gccggcgccg gcctcatcac ggtgggcggc atcatcttga tcgccacccg gtcccgccgc
      541 caccaggctc gggcctgacg tcccggcgct ccggcgccga cgtgaccttc gaacggtgcc
      601 ccgggtcttc ggcccggggc accgtcgcgc gctgaccggg cccggtccgg gtacaacagg
      661 gccatggaca tcgccatcac cgggagcacg ggcctcatcg gccaggccct cacgacctcg
      721 ctgcgagcgg ccggccacca cgtgatcccg gtcgtgcggt ccgccagcag ccccggcacc
      781 atccgctggg acccgggcgc cggcaccatc gacgcggcct cgctcgaggg cgtggacgcg
      841 gtggtgcacc tcgcgggaga aggcatcgca tcgggccgtt ggaccgatgc tcagcgcgac
      901 cgcatcagga ccagccggga gcggggcacc caggtgctcg ccaccgccct cgccgggttg
      961 gacgcgcccc cctcggtgct ggtgagcggc tcggccatcg gctactacgg cgaccgcggc
     1021 gacgacgtgc tggccgaagg ctccggtccg ggcggcgact tcctcgccgg cgtgtgcctg
     1081 gcgtgggaag gagccaccgc gcccgccgag gcggcgggca tccgcaccgt gcacctccgc
     1141 accggcatcg tgctcgacgc ccacggaggg gcgctcgcca agcagctgcc ggcgttcaag
     1201 ctcggcctcg gcgccaaggc gggctcgggt cgacagtgga tgtcgtggat caccctgcac
     1261 gacgaggtgc gcgccatccg cttcgccctg gaggaggcga gcctctcggg tgcgctcaac
     1321 ctcaccgcgc cgaacccggt gaccaacgca tcgttcaccg acgccgtcgg agacgcgctg
     1381 caccggccga cgttcctcac catccccgcc ttcgtccgca aggcgcccct cggcatcggc
     1441 gggctcgtgg agtcgctgct cctcgtgtcg cagcggtgcg aaccggccgc cctgctcacc
     1501 gccgggttca cgttcgacca cgaggacctc gccaccgccc tggcgtcggt cttgggtcgc
     1561 cgctgaggcc ggtggggatt cgccaccagc atcggcacgg ctcctagggt gaccggacca
     1621 tgtctggtcg ccgcatcgcc gccttcgact tcgacggaac cctgacccgg cgagacacgc
     1681 tgctgccgtt cctggcgcgg gcgtgcggcg ccacggcgtt cggccgcgcc gtcgggcaca
     1741 tcgctcccca ggcagtacgg gcccggaccg gtcggctcca agccgaggtc caccatcgag
     1801 atgtcaccaa ggccgccctg ctgcgcgagc tgctcgccgg acgggacgcg tcgtggtact
     1861 cggcacaggg tgatcagtac gccaccacgc tcggccccaa gctccgcccc gagatggtgg
     1921 agcaggtgcg ctggcaccga gacgccggcc acgagctcgt catcgtgtcg gcgtcgctcc
     1981 gcacctacct cgatccgttc gccacctcgt tcggcttcga ccacgtgatc gccgtcgacc
     2041 tcgaggcgga cgaccggggc gtcctcacgg gcgagatggc cgggccgaac gtgcggggcg
     2101 aggagaaggc ggtccgcctc cggcagtggc tgggcggcga ggccccggag atgatgtggg
     2161 cgtacggcaa ctccagcggc gacaccgagc tgctggcgat ggccgacatc gcggtgtggg
     2221 tcgccggccg gcgacggggc cagcacgcga acgaccgccc ggccgtcggg cccgggcggt
     2281 cgttctccga tcagcagacc gcctgagacc gctgcccgtg cgggcagcgg tcaggcgggt
     2341 ccgatcactt gggctgcatg cggatgccgc cgtcgatgcg gatcgactcg gcgttcatgt
     2401 agctgttcga cagcagctcg gcggtgaggc tggcgaactc ggccggctgg cccaggcgct
     2461 tcgggaacag cacgccgacc tcgaggcggg ccttgaactg ctccgaggcc tcgccctcgc
     2521 cgtagatcgg ggtgtcgatc aggccgggga ggatggtgtt cacgcggatg ccgacagccg
     2581 agaggtcacg ggcgatcggg agggtcatgc cgaccacgcc gcccttggag gcggagtacg
     2641 aggcctggcc gatctggccg tcctcggcgg cgaccgaggc ggtgttgacg atggcgccgc
     2701 gctcgccgaa ctcgtcgggc tcgttctgcg acatggccgt ggccgcgatg cggatgcagt
     2761 cgaaggtgcc gatcaggttg atctcgacga ccttcttgaa gatgtcgagg tcgtgggcgg
     2821 agctgtactc gccgtccttg ccgatggtgc gggtggccca gccgatgccg gcgcagttca
     2881 ccagggcctt gagcggaccg aggtccttgg ccgcctcgac cgcggcga
//
LOCUS       DATMFS010000405         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_84223, whole genome shotgun sequence.
ACCESSION   DATMFS010000405 DATMFS010000000
VERSION     DATMFS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84223"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..418
                     /gene="panD"
                     /locus_tag="VNQ33_07620"
     CDS             2..418
                     /gene="panD"
                     /locus_tag="VNQ33_07620"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWJ98012.1"
                     /translation="MMKSKIHRATVTDADLHYVGSVTVDQDLMDAADLVEFEQVAVVD
                     IDNGARLETYVIAGRRGSGDLCLNGAAARLVSPGDKVILISYADYEDAELATYEPAVV
                     HVDTANAIVDEATAAALAALHAPAPRRYVEVPAATI"
     gene            621..2210
                     /gene="nadB"
                     /locus_tag="VNQ33_07625"
     CDS             621..2210
                     /gene="nadB"
                     /locus_tag="VNQ33_07625"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWJ98013.1"
                     /translation="MTDTELDLLVLGSGVAGLSAAVRAAETHKMRVGVLTKGELPQAT
                     TRWAQGGVAAVLGGDPDSTDLHLADTLQAGAGLCDVDAVRVLVDEGPHRVNELITLGA
                     IFDRDTHGQLELAREGGHSLARIVHAGGSQTGAEVERALVEAVQATMAAIHEHAFALE
                     LIVEAGRCRGVTARFPDGSVQEVRATNVLVTTGGAGQLYAVTTNPLEATGDGIAMALR
                     AGVAVADIEFTQFHPTALSVEAMPRPLLTEALRGHGALLRDTTGERFVDELQPRDVVS
                     RAETRRMLELGADHVFLDATGLESFDTRFPNIAKELRAVGLDPATDWLPVAPAAHYTC
                     GGIVTDLRGSSSLPGLWAAGEASCSGVHGANRLASNSLLEGMVFGPRAVEAIEAGGSG
                     PEPTGAMRTVLGMDPDRPDEVPAGVIGGRFVHLEPVEAVDAAPEDPAALRTQMQHELT
                     AHAGVLRDAASLDQAAKVVADALVAARADTVADHEVRNLATVARAAVEAARRREESRG
                     AHTRDDFPDTRPELAHRIVFV"
BASE COUNT          354 a          964 c          938 g          320 t
ORIGIN      
        1 catgatgaag tcgaagatcc accgcgccac ggtgaccgac gccgacctgc actacgtggg
       61 gtcggtgacc gtcgaccagg acctgatgga tgcggccgac ctggtggagt tcgagcaggt
      121 ggcggtggtc gacatcgaca acggcgcccg cctcgagacc tacgtcatcg ccggccggcg
      181 cggctcgggc gacctctgcc tcaacggtgc cgccgcccgc ctggtgtcgc cgggcgacaa
      241 ggtcatcctc atcagctacg ccgactacga agacgccgag ctggccacct acgagccggc
      301 cgtcgtccac gtcgacacgg cgaacgccat cgtcgacgag gccaccgccg ccgccctcgc
      361 cgccctccac gccccggctc cccgccgcta cgtcgaggtc cccgccgcca ccatctgacc
      421 ggacgaacct cgtatgtcac cggccccgga tggtcgctct cgcagacgaa gttccgacgg
      481 gatggagcca ccccaccgaa cctcgtaggt cagaccgccc gaataggcgc tctcgcctac
      541 gaagttcgct tcggggtggg tcgtgtcgag aggcgggggt ggaggtggag gtgggtgagc
      601 cggcgggcca gactgctcgg atgaccgaca cggaactcga cctgctcgtc ttggggagcg
      661 gggtcgccgg gctctcggcg gccgtgcggg cggcggagac ccacaagatg agggtcggtg
      721 tgctgaccaa gggcgaactg ccgcaggcga ccacgcggtg ggcccagggc ggggtcgcgg
      781 cggtgctcgg gggcgacccc gactccaccg atctgcacct cgccgacacg ttgcaggccg
      841 gtgccgggct gtgcgacgtc gatgcggtgc gcgtgctggt cgacgagggc ccccaccggg
      901 tcaacgagct gatcaccctg ggtgccatct tcgaccggga cacccacggc cagctcgagc
      961 tggctcgcga gggcggccac tcgctggcgc gcatcgtcca cgccgggggc tcccagaccg
     1021 gcgccgaggt ggagcgggcg ctggtcgagg cggtgcaggc gacgatggcc gcgatccacg
     1081 agcacgcctt cgcgctggag ctgatcgtcg aggcgggccg ctgccgcggc gtcaccgctc
     1141 ggttccccga cgggtcggtc caggaggtcc gggccacgaa cgtgctcgtc acgaccggcg
     1201 gtgccggcca gctctacgcg gtgaccacga accccctgga ggccacgggc gacgggatcg
     1261 ccatggcgct gcgggccggg gtggcggtgg ccgacatcga gttcacccag ttccacccca
     1321 ccgccttgtc ggtcgaggcg atgccccggc cgctgctcac cgaggcgctc cggggccacg
     1381 gcgccctcct gcgcgacacc accggcgagc ggttcgtcga cgagctccag ccgcgcgacg
     1441 tcgtgtctcg tgccgagacc cgccggatgc tcgagctcgg cgcggaccac gtcttcctcg
     1501 atgccaccgg cctcgagtcg ttcgacaccc ggttcccgaa catcgccaag gagctgcggg
     1561 ccgtcgggct cgacccggcc accgactggc tgccggtcgc cccggcagcg cactacacgt
     1621 gcggcgggat cgtcaccgac ctccgcgggt cgtcctcgct gccgggactc tgggctgcgg
     1681 gtgaggcctc gtgctcgggt gtccacggcg ccaaccggct cgcctcgaac tcgctgctcg
     1741 agggcatggt gttcgggccg cgggcggtgg aggccatcga ggcgggcggg tcgggccccg
     1801 agccgacggg cgcgatgcgc accgtgctgg gcatggaccc cgaccggccc gacgaggtgc
     1861 ccgctggcgt catcggcgga cgcttcgtcc acctggaacc cgtcgaagcg gtcgacgccg
     1921 cgccggagga cccggccgcc ctgcgcaccc agatgcaaca cgagctcacg gcccacgccg
     1981 gggtcctgcg cgacgcggcg tcgctcgacc aggcggccaa ggtcgtggcc gacgcgctgg
     2041 tcgcggcccg cgccgacacc gtggccgacc acgaggtccg caacctcgcc accgtcgcgc
     2101 gcgctgcggt cgaggccgcc cgacgccgcg aggagagccg tggcgcccac acccgcgacg
     2161 acttccccga cacccgcccc gagctcgccc accgcatcgt cttcgtctga ccgaggtccg
     2221 cgccgggcgt cgtcgctcga tcggccgccg atcccctgga ccgcgccgcg caagggcggc
     2281 atcgccggga tcgggtcacg tcgccggcgc ggcgcggcac cggcgacccc ggtctcaggt
     2341 gagcggcccg agacgtccga tccgaccacg ctccgcagcg gttccgccgc ccacccggga
     2401 gaccgagcga cgggcggccc acccgcccgg gccgcgggcc gacgatcgcg gcagggggga
     2461 gccaggatcg ggcatcggcg ggaacgacgt ccggtatcgt cgggcgctcg gacggggcgt
     2521 gacgatcggg caggagctca acgatgaccg acaccgggga cggcgggcac cagctg
//
LOCUS       DATMFS010000406         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_130558, whole genome shotgun sequence.
ACCESSION   DATMFS010000406 DATMFS010000000
VERSION     DATMFS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130558"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VNQ33_07630"
     CDS             complement(<1..783)
                     /locus_tag="VNQ33_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98014.1"
                     /translation="MTDTLAAATSEETLDELQAWLDGNWDPDLTVAQWWELLGLAGWA
                     APTLPTNAYGKGVARSVGVQIQKAIAAYGALGAPAGLGLLLAAPTIADHGSQEQIDTM
                     VRDIVTGQKAWCQLFSEPGAGSDLAGLTCKAVRDGDEWIVTGQKVWTSLGQTADIGML
                     IARTAPDLPKHQGITYFGIDMVQPGIEVRPLREMTGHAMFNETFLDEARVPHANVIGG
                     LNNGWAVANSTLGHERAGLGAGGAGGGTGGATPGQIAGHLGKR"
     gene            1028..2230
                     /locus_tag="VNQ33_07635"
     CDS             1028..2230
                     /locus_tag="VNQ33_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98015.1"
                     /translation="MAERPSSPSDRRPPDRRPPDRRRPAAKAPRRHPIRRFNRRIPGW
                     SVPIVIIGVLLVAELGARVIGPDITRKAGTEERLFVKSDQIYQRGADRTDVVVFGSSE
                     TAGGLVPTTMAKEVPELPGIYNAALAGSSVSLMHEWAQRIVVPQLHPDVAVIGLLPMQ
                     VMKIDVTGAQGPEEALAAYRVAIEQVDPGGVGDLGWELRQRSDLIRYRPYLRSPEQAA
                     DGAWTALHGGLEVTPEDLEADAAMDWTKETDPGRVARTTEDDGSVHDYWSQSLPVDAD
                     ALGGQLYQRFGQGTVDLAALDAFVTDLEAAGVTPVIALAPVDRGPLTKGGADLTPLDD
                     VSRQVEAWGKERGIAVDNQFTQDWPSHLFHDRNHLDREGAKRWSTEVGSWLAELCGAG
                     EIPACAGG"
BASE COUNT          296 a          875 c          865 g          318 t
ORIGIN      
        1 ccgcttgccg aggtggccgg cgatctggcc gggggtcgcc ccgcccgtgc cgcccccggc
       61 gccgccggcg ccgaggccgg cccgctcgtg gcccagggtg ctgttggcca cggcccagcc
      121 gttgttcagg ccgccgatga cgttggcgtg ggggacgcgg gcctcgtcga ggaaggtctc
      181 gttgaacatg gcgtgaccgg tcatctcccg caggggccgg acctcgatgc cgggctggac
      241 catgtcgatg ccgaagtagg tgatgccctg gtgcttgggc aggtcgggcg cggtgcgggc
      301 gatgagcatg ccgatgtcgg ccgtctggcc gagcgaggtc cagaccttct ggccggtcac
      361 gatccactcg tcgccgtcgc gcaccgcctt gcaggtgagg ccggcgaggt cggagccggc
      421 cccaggctcg ctgaacagct ggcaccaggc cttctggccg gtgacgatgt cacggaccat
      481 cgtgtcgatc tgctcctggg agccgtgatc cgcgatggtc ggcgccgcga ggagcaggcc
      541 gaggccggca ggagcgccga gggcgccgta ggcggcgatg gccttctgga tctgcacgcc
      601 gacggagcgg gccacgccct tgccgtaggc gttggtgggc agggtcggtg cggcccagcc
      661 ggcgaggccg aggagctccc accactgggc gacggtgagg tccgggtccc agttgccgtc
      721 gagccaggcc tggagctcat cgagggtctc ttcactggtg gcggcggcga gcgtgtcggt
      781 catcggtgct tcccctggtg cgtggcgccc ctcggcgccg gtacgcgcac cgtacttgac
      841 cgctgggtca agtttccatc cggcacctgg cgcagcgccg agccccggcc gtgccgtcca
      901 ccgccggtgc agcgacgggg accgacctgg cgaaggtccg tccgccggcc cgcccgcctc
      961 gccgcggtcg gcgcgccccc atgcggtcga ggccgtcgcc ccggtggctc cccctaggct
     1021 ccgccccatg gccgagcgtc cgtcctcccc ctccgaccga cgcccgcccg accgacgccc
     1081 gcccgaccgc cgccgtccgg ccgccaaggc cccgcggcgc cacccgatcc gccggttcaa
     1141 ccgccggatc cccggctggt ccgtgccgat cgtgatcatc ggcgtcttgc tcgtcgccga
     1201 gctgggcgcc cgggtcatcg gccccgacat cacgcgcaag gccggcaccg aggagcggct
     1261 gttcgtgaag tccgaccaga tctaccagcg gggggccgac cgcaccgacg tcgtggtctt
     1321 cgggtcctcg gagacggcgg gcggcctggt cccgaccacc atggcgaagg aggtgcccga
     1381 gctcccgggc atctacaacg cggccctggc cgggtcgtcc gtctcgctga tgcacgagtg
     1441 ggcccagcgc atcgtcgtgc cccagctcca ccccgacgtc gccgtgatcg ggctgctccc
     1501 gatgcaggtc atgaagatcg acgtcaccgg tgcccagggt cccgaggagg cgctggccgc
     1561 ctaccgggtg gcgatcgagc aggtcgaccc cgggggcgtc ggggacctgg ggtgggagct
     1621 ccggcagcgc tccgacctga tccgctaccg cccgtacctg cgctcgccgg agcaggccgc
     1681 cgacggcgcg tggacggcgc tgcacggcgg cctcgaggtc accccggagg acctcgaggc
     1741 cgacgccgcg atggactgga cgaaagagac cgaccccggc cgcgtggcga ggaccaccga
     1801 ggacgacggc tcggtccacg actactggag ccagtccctc cccgtcgacg ccgacgccct
     1861 cggcggccag ctctaccaga ggttcggcca gggcacggtc gacctcgccg ccctcgacgc
     1921 cttcgtgacc gacctggagg ccgcgggcgt cacgccggtg atcgccctcg cccccgtcga
     1981 ccgcggcccg ctcaccaagg ggggcgccga cctcacgccg ctcgatgacg tgtcccgcca
     2041 ggtcgaggcc tgggggaagg agcggggcat cgccgtcgac aaccagttca cgcaggactg
     2101 gccttcgcac ctgttccacg accgcaacca cctggaccgc gagggtgcga agcggtggag
     2161 caccgaggtc ggctcctggc tcgccgagct gtgcggcgcc ggggagatcc ccgcctgcgc
     2221 cgggggctga gggcgggcgc tacgcggggg gcggacccat cacgacggtc ccgttctgac
     2281 cgccgaggcc gaccgagttc gacaggccga tccccggtgt ccacggccga ggcgccgagc
     2341 cggtgacgat gtcg
//
LOCUS       DATMFS010000407         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_223283, whole genome shotgun sequence.
ACCESSION   DATMFS010000407 DATMFS010000000
VERSION     DATMFS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223283"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VNQ33_07640"
     CDS             complement(<1..597)
                     /locus_tag="VNQ33_07640"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWJ98016.1"
                     /translation="MSSSSTSWSPASWREFPAAQQPNWPDEGALEAVLKAMSSLPPLV
                     FAGEARDLTKRLGQVANGEAFLLQAGDCAESFDAFGADAIRDKLKVILQMAVVLTYSS
                     GVPTVKVGRIAGQFAKPRSSDTEKIGDLEIPSFRGHLVNDITPTEAARIPDPQRLLQA
                     YHQSSSTLNLLRAFTKGGYADLARVHTWNQEFVASSVEG"
     gene            complement(659..1489)
                     /locus_tag="VNQ33_07645"
     CDS             complement(659..1489)
                     /locus_tag="VNQ33_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ98017.1"
                     /translation="MSDSVGQYLNEIGAVALLNAQEERELSQIIERGVAARERIAAGT
                     GTAEDRRLDRAAARAKDRFIRANLRLVVSIARRYPLPPGMELLDLVQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQAIGRALDQKASLVRLPGDRSASLRAALRQVSGDGDELD
                     DEHARLHRLATPTSLDRSVGDDDGSELVDLLADSAPGPEDLAVAAEEDRMVTSLLDVL
                     DGRARFAVEQRFGLHDGRKRSYREVGEELGVTAEAARRMVKRAVNTVRTEAATRTDAA
                     "
     gene            1669..2262
                     /gene="nadD"
                     /locus_tag="VNQ33_07650"
     CDS             1669..2262
                     /gene="nadD"
                     /locus_tag="VNQ33_07650"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWJ98018.1"
                     /translation="MVRGAKRRRIGVFGGTFDPPHIGHLVAAVDAQRELGLDVVLLVV
                     ANVPWQKVDSRAVSPAEDRLALVRAAVEDTPFLQVSDIEIRRGGESYTADTLADLRRE
                     EPDAELFVILGTDAAAGFATWERFEEVAEQATLVVVDRPGAPTAVDPRFDWVRVDIPE
                     LEISSTELRARVAAGRSIRFLTPAGVASAIADRSLYR"
BASE COUNT          322 a          785 c          844 g          367 t
ORIGIN      
        1 accctccacg ctggaggcga cgaactcctg gttccacgtg tgcacccggg cgaggtcggc
       61 gtagccgccc ttggtgaacg cccgcagcag gttcagcgtc gacgacgact ggtggtacgc
      121 ctgcaggagc cgctgcggat cggggatccg ggcggcctcg gtgggcgtga tgtcgttgac
      181 gaggtgcccc cggaacgacg ggatctccag gtcgccgatc ttctcggtgt ccgacgagcg
      241 gggcttggcg aactgcccgg cgatgcgccc gaccttcacc gtgggcacgc ccgacgagta
      301 ggtgagcacg accgccatct gcaagatgac cttgagcttg tcgcggatgg catcggcacc
      361 gaaggcgtcg aaggactcgg cgcagtcgcc ggcctgcagc aggaacgctt cgccgttggc
      421 cacctggccc aggcgcttgg tgaggtcgcg ggcctcgccg gcgaacacca gcgggggcag
      481 gctgctcatc gccttgagca cggcttcgag cgcgccctcg tcaggccagt tcggctgctg
      541 agccgcgggg aactcgcgcc aagacgccgg cgaccaggag gtggaggagc tgctcacagg
      601 aaaagggtgg tctaccgggt ggggaaacgc aaagtcgttt ccccgtcagc cggtgccgtc
      661 atgcggcgtc ggtgcgggtg gccgcttcgg tgcggaccgt gttcacggcc cgcttgacca
      721 tccggcgggc ggcctcggcc gtcaccccca gctcttcgcc gacctcgcgg tacgagcgct
      781 tgcgcccgtc gtgcaggccg aagcgctgct cgaccgcgaa ccgggcccgg ccgtcgagca
      841 cgtcgaggag gctggtgacc atgcggtcct cctcggctgc cacggccagg tcctcgggac
      901 ccggtgccga gtcagcgagc aggtccacga gctccgagcc gtcgtcgtcg ccaaccgagc
      961 ggtcgagcga ggtgggggtg gcgaggcggt gcaggcgggc gtgctcgtcg tcgagctcgt
     1021 cgccgtcgcc gctcacctgc cgcagggcgg cgcggaggct ggcggagcgg tcgccgggga
     1081 gccgcacgag gctggccttc tgatcgaggg cccggccgat ggcctggcgg atccagaagg
     1141 tcgcgtacgt ggagaacttg aagcccttcc gccagtcgaa cttgtcgacg gcgtgctcga
     1201 gcccgaggtt gccctcttgc accaggtcga ggagctccat gccgggaggc agcgggtagc
     1261 ggcgggcgat ggacaccacg aggcggaggt tggcgcggat gaagcggtcc ttggctcggg
     1321 ccgcggcacg gtccagccgg cggtcctcgg ccgtgcccgt gccggccgcg atgcgctcgc
     1381 gggcggccac gccccgttcg atgatctggg agagctcccg ctcttcttgg gcgttgagga
     1441 gcgcaacggc gccgatctca ttcaagtact gaccaacgga atcgctcatg acggcttctc
     1501 caacaacttc ggtgcggtga ccattcccgc cggtcgcact tcagagaggc gcctccggca
     1561 agttccttgc aggtaatttg gaaccgtgac ctgcgcacat ctatttcctc ggtatgcggt
     1621 gacacccgtc accgcctgcc ccgatgtccg ctggtcgatg gtcggctgat ggtccggggc
     1681 gcgaagcgac gccggatcgg tgtcttcggt ggaacgttcg atcctcccca catcggccac
     1741 ttggtcgccg cagttgatgc ccagcgtgaa ctgggcctag acgtggtgct gctggtcgtg
     1801 gccaacgtgc cgtggcagaa ggtcgacagc cgggcggtca gcccggccga agaccggctc
     1861 gccctcgtgc gcgcggcggt cgaggacacg ccgttcctgc aggtcagcga catcgagatc
     1921 cgccgaggcg gcgagtccta cacggccgac acgctggccg acctccggcg cgaggagccc
     1981 gacgccgagc tgttcgtgat cttgggcacg gacgcggccg cggggttcgc cacgtgggag
     2041 cggttcgagg aggtggccga gcaggcgacg ctggtcgtcg tcgaccggcc cggagccccg
     2101 accgcggtgg acccgcggtt cgactgggtg cgcgtcgaca tccccgagct ggagatctcg
     2161 agcaccgagc tccgggcacg ggtggcggcc ggtcgctcca tccgcttcct cacgcccgcc
     2221 ggggtcgcgt ccgccatcgc cgaccgttcc ctctatcgtt gacctgatga cctcggagac
     2281 gccccctccg tcgcggcgtt cccgccccgg tgccgacg
//
LOCUS       DATMFS010000408         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_340666, whole genome shotgun sequence.
ACCESSION   DATMFS010000408 DATMFS010000000
VERSION     DATMFS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340666"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VNQ33_07655"
     CDS             <1..366
                     /locus_tag="VNQ33_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98019.1"
                     /translation="DRRARALGSPAGVVIALEEGWCWASPGEGAAAALRIDVAAGSLT
                     VEPGATALAVVEADGSAFVVVAAGSAHLERGGDGAVIGRGTIVMIDPSGAAQTDAATD
                     AEIEADPIVAENLALDAEL"
     gene            complement(412..2106)
                     /locus_tag="VNQ33_07660"
     CDS             complement(412..2106)
                     /locus_tag="VNQ33_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ98020.1"
                     /translation="MHDVIITGGTVVDGTGAPAVRADVAVSGGVVTHVGSVEGPAART
                     VDATGRLVTPGFVDIHTHLDAQISWDPLGTSSCFHGVTSVVIGNCGVTFAPCRPEDRR
                     GLAEMMESVEDIPADAIMQGLSWEWETYGQYLSELDRRPKGLNVGGMIGHSALRRYVM
                     GDRALDTEPTAEDLTAMVDLVEEAMAAGALGLSSSRTILHTGADGQPIPGTFAGLDEL
                     LALAGVLGRHGRGVFETAAMLGSQNGATPESTVTEVGILAEISRVTGRPVSFGLTQTT
                     AGGGLHRQVLDLVAEHNATGARVHPQTTVRSVGVLFGLAHHTPYGGCPAWQALNGLSL
                     EERLASVRNPANRAQLIAEADGREGGIDPSLLFPLDAVPADYSLRPETSLAARAAAAG
                     TTPAALWLDLLDATDGRCLLVWPFLNQTPAEVEHLLGDPNVVLGLADAGAHVGQIMDA
                     SQPTWFLAHWVRDRRLHSIEEGIARLTSIPADLFAMPDRGVLAVGRPADINVIDLDAL
                     ALPLPTFAHDFPGGAGRWVQGAVGYDLTMVNGVVTVEGGEHTGELPGVTLRSTEAV"
BASE COUNT          278 a          765 c          843 g          292 t
ORIGIN      
        1 gaccgccgcg cccgtgcgct cggcagcccc gcgggcgtcg tgatcgccct cgaggagggc
       61 tggtgctggg cctcacccgg cgagggcgcc gccgctgcgc tgcgcatcga cgtggccgcg
      121 ggctcgctca cggtcgagcc gggggccacc gccctggcgg tggtcgaggc cgacggctcg
      181 gcgttcgtcg tcgtggcggc cggttcggca cacctcgagc ggggcggtga cggtgccgtg
      241 atcgggcgag gcacgatcgt catgatcgac ccctcgggtg cggcgcagac cgacgcggcg
      301 accgacgccg agatcgaggc cgacccgatc gtcgccgaga acctcgcgct cgacgccgag
      361 ctctgatcgg accgggcccg tcggcctcgc cgctgacgtc gccccgagcg gtcagacggc
      421 ctcggtgctg cgcagggtca cgcccggcag ctcgcccgtg tgctcgcccc cctcgacggt
      481 gacgacgccg ttgaccatgg tcaggtcgta gcccaccgcg ccctggaccc agcggcccgc
      541 gccgccgggg aagtcgtggg cgaaggtggg cagggggagc gcgagcgcat cgaggtcgat
      601 cacgttgatg tcggcgggtc gacccacggc gaggacgccg cggtcgggca tggcgaagag
      661 gtcggcgggg atcgacgtca ggcgggcgat gccttcctcg atcgagtgca ggcggcggtc
      721 ccgcacccag tgggccagga accacgtcgg ctggctggca tccatgatct ggccgacgtg
      781 ggctccggcg tcggccaggc cgagcaccac gttggggtcg cccagcaggt gctcgacctc
      841 ggcgggcgtc tggttgagga acggccagac caggaggcac cggccgtcgg tggcgtcgag
      901 caggtccagc cagagggcgg ccggggtggt gcccgcagcg gccgcccggg cggcgagcga
      961 ggtctcgggc cgcaacgagt agtccgccgg caccgcatcg agcgggaaga gcagggacgg
     1021 gtcgatcccg ccctcccgcc cgtcggcctc ggcgatcagc tgggcccggt tcgccgggtt
     1081 tcgcacggag gcgaggcgct cctccagcga cagcccgttg agcgcctgcc acgccgggca
     1141 gccgccgtag ggcgtgtggt gcgccaaccc gaacagcacg cccacggatc gcaccgtggt
     1201 ctgcgggtgg acccgggcgc cggtcgcgtt gtgctcggcc accaggtcga gcacctggcg
     1261 gtgcaggccc ccgccggcgg tggtctgggt caagccgaac gacacgggcc ttccggtgac
     1321 ccggctgatc tcggcgagga tcccgacttc ggtgacggtg ctctcgggcg tggcgccgtt
     1381 ctgcgagccg agcatcgccg cggtctcgaa cacgccccgg ccgtggcggc cgagcacgcc
     1441 ggccagcgcg agcagctcgt cgagccccgc gaacgtgccg gggatcggct ggccgtcggc
     1501 gcccgtgtgg aggatggtgc ggctcgagga gagcccgagg gcaccggcgg ccatggcctc
     1561 ctcgacgaga tcgaccatgg cggtgaggtc ctccgcggtg ggttcggtgt cgagcgcccg
     1621 gtcgcccatg acgtaccgcc gcagggcgct gtggccgatc atgccgccga cgttgaggcc
     1681 cttgggccgc cggtcgagct cgctcaggta ctggccgtag gtctcccact cccacgagag
     1741 cccctgcatg atcgcgtcgg cggggatgtc ctcgaccgac tccatcatct cggcgagacc
     1801 gcgccggtct tcggggcgac agggcgcgaa cgtgaccccg cagttgccga tgaccaccga
     1861 cgtcacaccg tggaagcacg acgaggtgcc gagcgggtcc caggagatct gggcgtccag
     1921 gtgggtgtgg atgtcgacga acccgggcgt gacgagcctg cccgtggcgt cgaccgtgcg
     1981 cgctgcgggg ccttcgacag agccgacgtg ggtgaccacg ccgccggaca cggcgacatc
     2041 ggctcgcacc gccggtgctc cggtcccgtc gacgacggtg cctccggtga tgatgacgtc
     2101 gtgcatgtgc tgctcctggg ccgtccctgg cgagtggggg ggggaacggc tcgtccccga
     2161 gccatccgcc cggcatcg
//
LOCUS       DATMFS010000409         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_268263, whole genome shotgun sequence.
ACCESSION   DATMFS010000409 DATMFS010000000
VERSION     DATMFS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268263"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VNQ33_07665"
     CDS             <1..1037
                     /locus_tag="VNQ33_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWJ98021.1"
                     /translation="PEDLFTVQTQMWAKYHVDDPDEFYNGNDEWSIPEDSGARKKSGG
                     TSTPIGPDGQKLTSGDRYPSQYVLMQLPGEDEARFVLQRPYVTSTDDSNSSAGEGQLR
                     ALIVADRDGSRATLKTYTLPASDPPDGPNLAAADMLANGDVAAKVKDLCTDKRVCTFA
                     APSILPVGNSLMYVQTFLSAADKSHAGKIEYVIVNYRRPGDQSEVKLDTTLYGALEQL
                     FGDKVPTSVQGGDAASAPDGSTNPDEPDDGEQSSGTLTEQESRLIDQLVKAFDDADAA
                     AREGDQVTYAERITEAGRLAGQLQKLRRESEPDSGPKGDGSGKDTTTTQPKTTTTTEP
                     ATTTTTSAGT"
     gene            1136..1212
                     /locus_tag="VNQ33_07670"
     tRNA            1136..1212
                     /locus_tag="VNQ33_07670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Met,seq:cat)
     gene            complement(1353..1679)
                     /locus_tag="VNQ33_07675"
     CDS             complement(1353..1679)
                     /locus_tag="VNQ33_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98022.1"
                     /translation="MSKTPPSDLSTWRMTLTCDHAVECEQHRSNSYWSSRVKECPECG
                     SHRGIVTAVQLPRAEPEEIGQQERADVQHAATQRELAKLRREEAVLRERIAALERLNG
                     TARRHE"
     gene            1798..2178
                     /locus_tag="VNQ33_07680"
     CDS             1798..2178
                     /locus_tag="VNQ33_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98023.1"
                     /translation="MMARLEVSIGCVSFDSFDSFDSFDSFLRGQMHERRPVPQVVDDG
                     QIAAAAGHSGLQFLRRETGALRAPRLVPGFHVVVFGFSRQRFGATPVQRASTLLFFSG
                     SCHERIVVQDCLRSVCPCPRRALE"
     gene            2202..2543
                     /locus_tag="VNQ33_07685"
     CDS             2202..2543
                     /locus_tag="VNQ33_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWJ98024.1"
                     /translation="MCDDLLVGGIELHDEVVTWMDALDGNEWDRTVVVIDRLATLGST
                     ARMPLSRSLGDGLFELRFTLGPTARLITYRFTKDGRIVLLTTFRKQRNNERNEIARAR
                     TAAEACARRNP"
     gene            2555..2875
                     /locus_tag="VNQ33_07690"
     CDS             2555..2875
                     /locus_tag="VNQ33_07690"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ98025.1"
                     /translation="MANYSRWDDVKNKRRGPDAETRAGVEQDLALGQLIYDLRTEAGL
                     SQRQLAEKMGTTQSVISRLEEGGGARNRIDTLARVATALDRHLVVSFPEKVPTKLIDA
                     VQVA"
     gene            3705..>4946
                     /locus_tag="VNQ33_07695"
     CDS             3705..>4946
                     /locus_tag="VNQ33_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HWJ98026.1"
                     /translation="MLLTISTEHAPATDLGFLLHKHPDNVRTVAFPFGDAHVFFPEAG
                     DQRCTAAVLVEVDPIGLIRRRGRKGQESFSLAGYVNDRPYVASSFLSVVVGKVFGTAM
                     TGRCDQRPELADAALPFEVEVPVLPIRGGEPVLRSLFEPLGYQIEAAPIPLDDAFPAW
                     GPSRYWSVRLAGEVRLADLLSHLYVLLPVLDDDKHYWVGEDEARKLLDKGGAWLASHP
                     ERELISRRYLKHRRSLANDVLARLADDAPDDQVEVDDEHDREEQQVEERISLNQQRLD
                     VVAAAVVDAGASRVLDLGCGEGRLVQALLKVPQVAHVTGADVSTRVLDRAADRICIDD
                     LSDRQRERVALIQAGLTYRDSRFAGFDAATLVEVIEHLDEPRLAALEQVVFAHASPST
                     VIVTTPNREHNVRFESLPAGQF"
BASE COUNT          803 a         1627 c         1697 g          819 t
ORIGIN      
        1 accccgagga cctcttcacg gtgcagaccc agatgtgggc gaagtaccac gtcgacgacc
       61 ccgacgagtt ctacaacggc aacgacgagt ggagcatccc ggaggactcg ggcgcgagga
      121 agaagtccgg cggcaccagc acgccgatcg gacccgacgg ccagaagctc accagcggtg
      181 accgctaccc ctcgcagtac gtgctgatgc agctacccgg cgaggacgag gcccggttcg
      241 tgctgcagcg cccgtacgtc acctcgaccg acgactcgaa ctcgagcgcc ggcgagggcc
      301 agctccgagc actgatcgtc gcggaccgcg acgggtcgcg ggcgacgttg aagacctaca
      361 cgctgccggc ctccgacccg cccgacggcc cgaacctcgc cgctgccgac atgctggcca
      421 acggcgacgt ggcggccaag gtgaaggacc tctgcaccga caagcgggtg tgcacgttcg
      481 cagcgccgtc gatcctgccg gtcggcaact cgctgatgta cgtgcagacg ttcctgtcag
      541 cggccgacaa gagccacgcc ggcaagatcg agtacgtcat cgtcaactac cggcgtcccg
      601 gcgaccagtc cgaggtgaag ctcgacacca ccctctacgg ggcgctcgag cagctcttcg
      661 gcgacaaggt ccccaccagc gtccaaggcg gggacgccgc ctccgcgccc gatggcagca
      721 ccaaccccga cgagcccgac gacggcgagc agtcgagcgg cacgctcacc gagcaggaga
      781 gccggctcat cgaccagctg gtgaaggcgt tcgacgatgc cgacgccgcc gctcgcgagg
      841 gcgaccaggt cacctacgcg gagcgcatca ccgaagccgg ccgcctcgcc ggccagctcc
      901 agaagctccg tcgggagagc gagccggact ccggccccaa gggcgatggc agcggcaagg
      961 acaccacgac gacccagccc aagacgacga ccaccaccga accggccacc accacgacca
     1021 cctcagccgg cacgtagccg caggtcagca acatcgttcg agccgggagc ggccggtatc
     1081 gcaagggagc caggttgcag ttcacgaggg tccgtcggta aggtcgtcag cacgccgcgg
     1141 ggtggagcag tctggtagct cgtcgggctc ataacccgaa ggtcgcaggt tcaaatcctg
     1201 cccccgccac catctaacga aagccccagg tcgctgacct ggggctttgt cgttgggagg
     1261 cggttgcgtc agtcagacca gccggaacgt ccctggtaga ggagttgcag gttcctatcg
     1321 acccccgctg cgttcttgat ggatcgtcgg cgctactcat gtcggcgagc agttccgttc
     1381 agacgttcca aggctgcgat gcgttcccgc agcacggctt cctcgcggcg cagtttggcg
     1441 agctcgcgtt gggtggccgc gtgttgtacg tctgcgcgct cttgttgccc gatctcctcg
     1501 ggttcggccc ttggcagctg tacggcggtg acgatgccgc gatggctgcc gcactcgggg
     1561 cactccttga ctcgtgaaga ccagtaggag ttcgaacggt gctgttcgca ctcgacggcg
     1621 tggtcgcagg tcaacgtcat ccgccacgtg ctgaggtcgc ttggtggcgt cttgctcacc
     1681 gacgaaacga ggcgagcgat cgcggcggcc tgcgcagggc tgagtggtgg tggcggacag
     1741 caattgccgc agtgtggcgc tcgagcctgc gacgctccag cgaaggctat ggcagccatg
     1801 atggcgcgcc tggaagtcag catcggctgc gtctcgttcg actcgttcga ctcgttcgac
     1861 tcgttcgact cgttcctccg cggtcagatg catgagcgcc ggccagtccc ccaggttgtc
     1921 gatgatgggc agatcgcagc cgcggcaggc cactccggcc tccagttcct caggcgtgag
     1981 acgggtgccc tccgtgcgcc acgcctcgtg cctggcttcc atgtggtggt gttcggcttc
     2041 agccgccagc gcttcggtgc gacgccggtt cagcgcgcgt cgacgcttct gttcttcagt
     2101 gggtcttgcc atgagcgcat cgtggtgcag gactgcctcc gatcggtgtg cccgtgccct
     2161 cgccgcgcgc tcgaatgatc gctcttgtac gatgatcgtg tttgtgcgat gatctgctgg
     2221 tgggtggcat cgagctccac gacgaggtgg tgacctggat ggacgcactc gacggcaacg
     2281 agtgggatcg gacggtggtg gtcatcgacc ggttggccac cctcggttcc accgctcgga
     2341 tgccgttgtc gaggagcctc ggcgacggct tgttcgagtt gcggttcacg ctcgggccga
     2401 cggctcggct gatcacctac cggttcacca aggacgggcg gatcgtgctg ctcaccacgt
     2461 tccgcaagca acgcaacaac gaacgcaacg agatcgccag ggctcggacc gcagccgagg
     2521 cctgcgcccg ccggaacccg tgaaggagac gaccatggcc aactactccc gctgggacga
     2581 cgtcaagaac aagcgccgag gccctgatgc ggagacccgg gctggcgttg agcaggatct
     2641 cgcgctcggc cagctgatct acgacctgcg caccgaagcc ggcctcagcc agcgccagct
     2701 ggccgagaag atgggcacga cccagtcggt gatctcccgg ctcgaggagg gcggcggtgc
     2761 ccgcaaccgc atcgacaccc tcgcccgcgt cgccaccgcc ctcgaccgac acctcgtcgt
     2821 ctcgttcccc gagaaggtcc ccaccaagct tatcgacgcc gtccaagtcg cctgagtcgg
     2881 aggattctgc tgcaggagtt ccgggtagcg aagggcggag cgtcgcgggt gctattcatt
     2941 cgatccccag caccggtacc tgtcagccgt tgcgcccggt ctcaatcgtg gcgcttcgcg
     3001 ggaatcaccg ctgagcactc atatggcacg cccggtagca gtcgaagacc accgagtcat
     3061 cggaacagcg ctcgaccatg agtacgtggg tgggcggtga ggagggcggc gaactgtctg
     3121 gctgcggcgg cgctcatgcc ggggaggagg tactggtagg tatgcatggt gaacacgacg
     3181 ttggcgtgac tgaggcgttc gctgacgacc ttgtgaccgg gaactcgacg gggtggccgg
     3241 cgatgctctg gatggtgcgg ctgatcgaga ggcgctggtt cgccccgtcg aggtcggacc
     3301 gcttgagtcc ggcgacttcg cctcggcgca ttccggtgta ggacgtgagg tggaggggcg
     3361 gggtagagcc gctgggaccg tgctgcggtg aggaagacgg cgagctcttc ggcgctccag
     3421 gcccgtggga tcctgagggt gggtcgcttg gggcttgcgc cggtggcgtg ggcgacgttg
     3481 gtgtcgagga gttgtcgccg cactgcgagg tccagcgacg agcgcatgat gacatggacc
     3541 tcgtgcacgg tcttgggggc gaggccggtt ccgtgacggg tcgccggtcg ccggtcgccg
     3601 gtcgtgacgg gctcggtcgt cggaagcggg agcccgaccg aaacggccag cggcgcccgg
     3661 ggcacgttgg gccatgatgg tcgaccaccc actggcggtc ttccatgctc ctcacgatct
     3721 ccaccgaaca cgcgccagcg actgatctgg gcttcctgct gcacaagcac ccggacaacg
     3781 tgcgtactgt cgcgttcccg ttcggcgatg cgcacgtgtt cttccctgaa gcgggagacc
     3841 agcgctgcac cgctgcggtg ctcgtggagg tcgacccgat cgggctgatc cgcaggcgag
     3901 gccggaaggg tcaggagtcg ttctcgctgg cgggctatgt gaacgaccgg ccctatgtgg
     3961 cgtcgtcgtt cctgagcgtc gtggtcggga aggtgttcgg caccgcgatg acggggcgat
     4021 gcgaccagcg tcccgagctc gccgacgctg cgttgccgtt cgaggttgag gtgccggtct
     4081 tgccgatccg tggcggcgag ccggtccttc gttcgctgtt cgaacccctc ggctaccaga
     4141 tcgaagcagc gccgatcccg ttggatgacg cgttccctgc ctgggggccg agccggtact
     4201 ggtcggtgcg gttggccgga gaggtgcgcc tcgcggatct gttgtcgcac ctgtacgtgc
     4261 tgttgccggt gctcgacgac gacaagcact actgggtcgg tgaggatgaa gcccgcaagc
     4321 tcctcgacaa gggtggcgcc tggctggcat cgcatcccga gcgggagctg atcagccgcc
     4381 gctacctcaa gcaccgtcgt tcgctcgcca acgacgtcct cgctcgcctc gccgacgacg
     4441 cgccggacga tcaggtcgag gtcgacgacg aacacgaccg cgaggagcaa caggtcgagg
     4501 agcgcatcag cctgaaccag cagcgcctcg acgtcgtcgc cgcggctgtc gtggatgcag
     4561 gagcctcacg ggtgctcgac ctgggctgtg gcgaggggcg cctggtgcag gcgctgctga
     4621 aggtgccaca ggtcgcccac gtgaccggcg ccgatgtgtc gacacgggtg ctcgatcggg
     4681 ccgctgaccg catttgcatc gacgacctgt cggaccgcca gcgtgaacga gtcgctctga
     4741 tccaggccgg gctcacctac cgcgacagcc gcttcgcggg gttcgacgcg gccacgctgg
     4801 tcgaggtgat cgaacacctc gacgagcccc gcctcgccgc tctcgagcag gtcgtcttcg
     4861 cccacgcgtc accgagcacg gtgatcgtca ccacgccgaa ccgagagcac aacgtccggt
     4921 tcgagtcgct acccgccggt cagttc
//
LOCUS       DATMFS010000410         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_84440, whole genome shotgun sequence.
ACCESSION   DATMFS010000410 DATMFS010000000
VERSION     DATMFS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84440"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VNQ33_07700"
     CDS             complement(<1..194)
                     /locus_tag="VNQ33_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98027.1"
                     /translation="MELTTSKARRFAAGLVLVATAVAGQGLSTPAGGAAGGPPGATTS
                     AAAPTPTAAVPGKPTVVAAVG"
     gene            526..768
                     /locus_tag="VNQ33_07705"
     CDS             526..768
                     /locus_tag="VNQ33_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98028.1"
                     /translation="MARKFAAAHGLGDDDVIVDRTQLEALQDALYCLQAAIEDVDNDL
                     EGKPSAAEVRTAIGWLLDNARPVAAARIEPRADAGT"
     gene            complement(966..1784)
                     /locus_tag="VNQ33_07710"
     CDS             complement(966..1784)
                     /locus_tag="VNQ33_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98029.1"
                     /translation="MDGQITAAVGYNGAGKTLCIVDRVIRPSLDAGRPVLSNTPIFAG
                     PDDAHLPWQQRAPHPLYVPLLSWRQLSSPLHGVTIFLDEISSMFDSRDSGRMPTQITS
                     RFNQLRKADNLVCWTAPDWDRCDKVLRRVTRRLWYCKGYLPQAAVGPDGEPLVWKSNR
                     LFRWKFYDAQKFEEFSTSQADSSRKGTLRSDDARWHRRSRHQSQFLYDTYAEVNLLDH
                     LDETGKCVDCGGTRRALKCECPPARNAGVVQERWDLDRMQAFAAELLLARTVTS"
     gene            complement(2166..2432)
                     /locus_tag="VNQ33_07715"
     CDS             complement(2166..2432)
                     /locus_tag="VNQ33_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98030.1"
                     /translation="MIVEAILDFIFAGIRWVISLVPAWTLPSFLLSPLSALTTLAANV
                     AWMGNWLPWNVIVGGMALMGSVRLVMAAVEFVMKVYAMIRGGAS"
BASE COUNT          432 a          791 c          889 g          352 t
ORIGIN      
        1 ccgaccgcag cgacgaccgt cggcttgcca gggaccgccg ccgtgggggt gggcgcggcc
       61 gccgacgtcg tcgcgccggg cggccccccg gccgcgccgc cggccggcgt ggacaggccc
      121 tggcccgcga cggccgtcgc caccaggacc agtcccgccg cgaaccggcg cgccttcgag
      181 gtcgtgagct ccacagcttc cccctgtcga tctgaccgcg gaccccctgg acccggtcgg
      241 tcgggactgt accgcgccgg tggccctcga acggttgaca tcatcgtcag gcccccggtg
      301 gtttcgtcac gtgacggaac ggggcaccgg gatcgcgggg cccgcgggga agggcggccg
      361 gccgccagca tcccgcctcg acccgacgga ggcgggtagt ttcatcacgt gacgaaacga
      421 acgatgggtg agcggtgccg gtggtgcggg caggtgctgc cggatcggaa cgcgacgggg
      481 cggccccgga ggtactgccg tcccgggtgc cgccaacagg cccacatggc gaggaagttc
      541 gccgccgccc acggcctggg cgacgacgac gtgatcgtcg accggacgca gctcgaagcg
      601 ctccaggacg cgctgtactg cctgcaagcc gcgatcgaag acgtcgacaa cgacctggag
      661 ggcaagccct cggcggccga ggtgcgcacg gcgatcggct ggctgctcga caacgcccgg
      721 cccgtcgccg ccgcccgcat cgagcccaga gccgacgccg gaacctgatt ccttactcga
      781 cataacgtag attatgggca gttgccgtgt tctctactgt ccgccagact ggagatgtcg
      841 acgccgttgc tcgtttcggc gtggacgagg gctcggtgct ggtaacaccg gtctccggtc
      901 gggccaggct tacccctggc ccgaccacta cccggatccg tccatggtcg gcacagaggt
      961 ggcggtcatg aggtaaccgt gcgggcgagg agaagttcgg ccgcgaatgc ctgcatgcgg
     1021 tcgaggtccc aacgctcctg cacgactccg gcgttgcggg cgggcgggca ctcgcacttg
     1081 agggcacggc gggtgccgcc gcagtcgacg cactttcccg tctcatcgag gtggtcgagg
     1141 aggttgacct cggcgtaggt gtcgtagagg aactgcgact ggtgccggga gcggcggtgc
     1201 caccgggcat cgtcggagcg cagggtgccc ttgcggctgg agtcggcctg cgaggtggag
     1261 aactcctcga acttctgtgc gtcgtagaac ttccagcgga acagccggtt ggacttccac
     1321 accagcggtt cgccatcggg tccgacagca gcctgcggta ggtagccctt gcagtaccag
     1381 aggcgcctcg tgacgcgtcg caggaccttg tcgcagcggt cccagtcggg agcggtccag
     1441 cagacgaggt tgtcggcctt gcgcagttgg ttgaacctgg atgtgatctg ggtcggcatg
     1501 cgtccggagt cccgagagtc gaacatggac gagatctcat ccaggaagat cgtgacaccg
     1561 tgcagcggcg atgacagctg ccgccacgag agcagcggga catagagagg gtgcggtgca
     1621 cgctgctgcc aagggaggtg agcgtcatct ggaccggcga agatcggggt gttggagagg
     1681 accggacggc ccgcatcgag actggggcgg atgacacgat ccacaatgca cagggtcttg
     1741 ccagcgccgt tgtagccgac ggcggcggtg atctgtccgt ccacgtcagc ccctcccctc
     1801 atcgggtgga acgacccaac tgaccggctc gacacggatg gcggtccggt cctcgccgct
     1861 tcgcagctgc ggtccggccg ccatctcagc gagagtcggt acatcgaggc ggcggcaaac
     1921 ccgaagagcg agtcgacggc aaacggcagg cccgagccag aggacgccga gcgggacgat
     1981 gacagctggg gcgggggcgg cggtgtgggc gacggaggcc acagcgacca cagtccccat
     2041 ctggacaccg acgaggaagt ggagccagcg gtcccagcgg cgtacccatg ccagacgcag
     2101 aggaagcgaa gatcccagag cgacggcaag atctttcgta acacctcgtc gagcggctac
     2161 gaaggtcacg atgcaccgcc tcggatcatg gcgtagacct tcatgacgaa ctcgacggcg
     2221 gccatcacta gccggacgga ccccatcaag gccatgccgc cgacgatgac gttccagggg
     2281 agccagttgc ccatccaggc gacgttggca gcgagcgtcg tgagagcgct gaggggcgag
     2341 agcaggaacg acgggagagt ccaggccggg acgagggaga tcacccatcg gattccggcg
     2401 aagatgaagt cgaggatggc ttctacgatc acgattcgtc accgccggaa cggatcgggg
     2461 cgac
//
LOCUS       DATMFS010000411         5630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_38134, whole genome shotgun sequence.
ACCESSION   DATMFS010000411 DATMFS010000000
VERSION     DATMFS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38134"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..722)
                     /locus_tag="VNQ33_07720"
     CDS             complement(51..722)
                     /locus_tag="VNQ33_07720"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HWJ98031.1"
                     /translation="MLSQVIGQFLPSAVGVAISPIPIIAVVLMLGTPKARTDGPAFAA
                     GWIVGMVAVSAVVLLLTSSADTSSAANDGVDWLKVAIGVLFLVLAKRQWDARPAKGEQ
                     PEMPGWMRKIDELEPGRAFTLGAALSGLNPKNLALTAAAAATISQAGLETGDTVVAVL
                     VFVVIASITVVGAVIFYLAAEERARAPLASVKEFMADHNAVIMMVILVILGAKILGEG
                     FGGLG"
     gene            890..1693
                     /locus_tag="VNQ33_07725"
     CDS             890..1693
                     /locus_tag="VNQ33_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HWJ98032.1"
                     /translation="MDGRDDRLDGIEGLLVDIDGVLTLSWKAIDGASEAVARLRSAGV
                     PVRYLTNTTSITRGEIVDRLGAAGMAVAAGEVFTAPVATASWIAAHHPEARCHLLNSG
                     DLVADLAGIDIVDGDGPADLVVLGGAGPEFSYEALNRAAGLLLDGAALVAMHRNRYWR
                     TDAGFALDTGAFVAALETAAGVTATVVGKPSPAFFATAVASLDLPVSAIAMVGDDVEN
                     DVLAAQGAGLAGILVHTGKYRPEALAAASGDPDHQIPSFATLPDLLGLP"
     gene            1698..2135
                     /locus_tag="VNQ33_07730"
     CDS             1698..2135
                     /locus_tag="VNQ33_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98033.1"
                     /translation="MARDAPPPTPVQHAARILLGAALVSAGIAHLTFARTDFLAQVPE
                     WVPLDADLVVVLSGIVEIALGTGLIVLARLRVPLGWTAAAFFVAVFPGNLSQLADGDD
                     AFGLDSGALRAVRLLFQPVLVAWALWCTGAWTTWRCRRGRDSG"
     gene            complement(2168..3133)
                     /locus_tag="VNQ33_07735"
     CDS             complement(2168..3133)
                     /locus_tag="VNQ33_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ98034.1"
                     /translation="MQAVDGGHPTWNRLGLFVCPTCRAGLSERDGLVACEAGHEFPLV
                     GDKAVLIGEGSSFVPGDVAATKASYFDARARESARKGEWRRRLPAPGRNIRGAFVAPA
                     QQIVAEVAEGRTPVGLLLGCGERGASRTERFAGVDWILTDVDLNYAAEAAADVTSLPL
                     PDGSVDIVLVEHVLEHVLDPLRGAAEIERVLRVGGVVVAVVPFSFPWHGAPIDYFRVT
                     PSGMRVLFRSCAVEHLGAGMGGGSALSYGAVATVAGIPVSRTWRRVALVLSRLLLTPI
                     KYLDQLEERRGKALTFAAEYQFVGRKLEEPLTDRELLDDVRARFG"
     gene            3291..>5630
                     /locus_tag="VNQ33_07740"
     CDS             3291..>5630
                     /locus_tag="VNQ33_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWJ98035.1"
                     /translation="MSRGEPFIRRGVVITLVALVAALVPLSTGIASAQTEPAVERAGN
                     LSPRLEDLASVGAVATSDAAENRAIDLPATGAGSLLHQPDGSFLANARLTTVDAATEA
                     ALQRAGARIVARSAVDHLVTVAIDSTDIERVAAVAGVEWLEEVLEPRVGPADLQGVAA
                     SLGEDGVATNGSCAAKVTSEGDTHLGAATARSTYGVDGAGVKIGVLSDSYNNLGGAAT
                     DVSNGELPGAGNPCGYTTPVVVQSDKASGGTDEGRAMAQAVHDLAPGATIMVATAFNG
                     DVDFAQQIRNLATAGAKVIVDDISYFNEPVYQDGIIAKAIADVTAAGVTYFSSAANSN
                     IILGGRDVASYEAAGYRPTTCPGTVTSQTGDADCHDWDPGAGADGGNLLTVSSNGQLQ
                     VKLGYSEPKFGITTDLDLLLLDNATNAIVAYSVSDNVNSSKDAYELLTYTNSSGAAKT
                     YRLVIGRYAGGTPRFKTIIQTGTGLTAVQWNASSGTDVVGPTAYGHNMMRGAGSIAAI
                     RYNSTTAPETFSSRGPATYCWNPVVGTTAATALASCQSDTIDVAATDGAANSFFGQLS
                     SGTWRFYGTSQAAPHAAAVAVLQLAARPCRTPAEILAAQRASGLAVGSYGVDAVGGGR
                     VDASAAISGLAACQAVPDAPTAVTGTPGNGQVTLSWTAPAGSGTNAVTAYRVTPFIGA
                     SAQAPVVFNSTATTQTITGLTNGTAYTFKVAATNASGTGADSAPSAAVTPRTVPGAPT
                     ALTGSAGDSSATLNWTAPASNGGSAVTGYTVTPYIGATAQ"
BASE COUNT          826 a         2180 c         1885 g          739 t
ORIGIN      
        1 ccagcgcctc ggcgggttcc ggtgggacga acggcgtctc gtcggccatg tcagcccagc
       61 cctccgaagc cctcgcccag gatcttcgcc ccgaggatca cgaggatgac catcatgatc
      121 acggcgttgt ggtccgccat gaactccttg accgacgcca acggcgccct cgccctctcc
      181 tcggccgcca ggtagaagat cacggcgccg acgaccgtga tcgatgcgat caccacgaac
      241 acgagcacgg cgacgaccgt gtcgcccgtc tccagaccgg cctgggagat ggtggcggcg
      301 gccgccgcgg tgagcgccag gttcttcggg ttcaggccgg agagggcggc gccgagcgtg
      361 aacgccctgc cgggctcgag ctcgtcgatc ttgcgcatcc agcccggcat ctcgggttgc
      421 tcgcccttcg ccggacgggc gtcccactgc cgtttggcca agaccaggaa cagcacgccg
      481 atggcgacct tgagccagtc gacgccgtcg ttcgcggcgc tgctcgtgtc cgcgctgctc
      541 gtgaggagga ggaccacggc gctgaccgcg accatgccca cgatccaccc cgcggcgaac
      601 gccggaccgt cggtgcgcgc cttgggggta cccagcatca agacgacggc gatgatcggg
      661 atcgggctga tcgccacccc cacggccgag ggcaggaact gcccgatcac ctggctcaac
      721 atggcgtgct ccctccacgg cggcgcgcgc tgcgaccgac ccggtcgagc gtagcgacgg
      781 cccggttgac cagcctggat gcaggtggcc gccggcgcga tcgccggcgt gacgaccggg
      841 cgcgctcgat cggacgcgtc gagccgcccc gctccgtacc ctcaggggca tggacgggcg
      901 cgacgaccgg ctcgacggca tcgaggggct cctcgtcgac atcgacgggg tgctgaccct
      961 ctcctggaag gcgatcgacg gggcgtccga ggccgtcgcc cggctgcgga gcgccggggt
     1021 gcccgtgcgg tacctcacga acacgacctc gatcacccgc ggtgagatcg tcgaccggct
     1081 cggtgccgcg ggcatggcgg tcgccgcagg cgaggtgttc accgctcccg tcgccaccgc
     1141 gtcgtggatc gccgcccacc atcccgaggc ccgctgccac ctcctcaaca gcggcgacct
     1201 cgtcgcggac ctcgccggga tcgacatcgt cgacggcgat ggaccggcgg acctggtcgt
     1261 cctcggcggc gccggccccg agttctccta cgaagccctc aaccgagccg ccggcctgct
     1321 cctcgacggc gccgccctgg tcgccatgca ccgcaaccgg tactggcgca ccgacgccgg
     1381 gttcgccctc gacacgggag cgttcgtcgc cgccctcgag acggcggcgg gggtgacggc
     1441 cacggtggtg ggcaagccga gtccggcctt cttcgcaacc gccgtggcct ccctcgatct
     1501 gccggtctcg gcgatcgcca tggtcggcga cgacgtcgag aacgacgtgc tggccgcgca
     1561 aggagccgga ctcgccggca tcctcgtcca caccggcaag taccgccccg aagccctcgc
     1621 cgccgccagc ggcgatcccg accaccagat cccctccttc gccacgctcc ccgatctcct
     1681 cggcctcccc tgatcacgtg gcccgagacg cacccccgcc gaccccggtg cagcatgcgg
     1741 cgcggatcct gctcggcgcc gcgctcgtct ccgccgggat cgcccacctg accttcgccc
     1801 ggaccgactt cctcgcccag gtgcccgagt gggtcccgct cgacgccgac ctcgtggtgg
     1861 tgctgtcggg gatcgtcgag atcgctctgg gcaccggcct catcgtgctg gcccggctgc
     1921 gggtcccgct cggctggacc gccgccgcgt tcttcgtcgc cgtcttcccg gggaacctgt
     1981 cgcagctcgc cgacggcgac gatgcgttcg ggctcgactc cggtgccttg cgcgccgtgc
     2041 gcctgctctt ccagcccgtc ctggtcgcct gggccctgtg gtgcaccggc gcctggacaa
     2101 cgtggcggtg ccgccgtggc cgcgacagcg gctgacgggt gcgggcccgg tctgagccga
     2161 ggcgacgtca gccgaagcgg gcgcgcacgt cgtcgagcag ctcccggtcg gtcagcggct
     2221 cctcgagctt ccgcccgacg aactggtact cggcggcgaa cgtcagcgcc ttgcccctcc
     2281 gctcctcaag ctggtcgagg tacttgatcg gggtgagcag gagccggctc aacacgagag
     2341 cgacccggcg ccacgtgcga gacaccggga tgccggcgac cgtggcgacc gcgccgtagg
     2401 agagggccga cccgccgccc atcccggcgc cgaggtgctc gacggcgcac gagcggaaca
     2461 ggacgcgcat ccccgacggc gtgacccgga agtagtcgat cggggcgccg tgccacggga
     2521 acgagaacgg gacgaccgcc accacgacgc caccgacccg caggacccgt tcgatctccg
     2581 ctgccccgcg aagcggatcg agcacgtgct cgaggacgtg ctccacgagc acgatgtcca
     2641 ccgagccgtc gggcaacggc aggctcgtga cgtcggcggc ggcttcggcg gcgtagttga
     2701 ggtcgacgtc ggtgaggatc cagtcgacgc ccgcgaagcg ctccgtgcgc gacgcgcctc
     2761 gctcgccgca gccgaggagc aggccgaccg gcgtccgccc ttcggcgacc tcggcgacga
     2821 tctgctgggc tggcgccacg aaggcacccc ggatgttgcg cccgggagcc gggagccgac
     2881 gccgccactc ccccttgcgg gcggactcgc gggcacgggc gtcgaagtag ctggccttcg
     2941 tcgcggcgac gtcaccgggc acgaacgacg acccctcgcc gatcagcacg gccttgtcgc
     3001 ccaccaaggg gaactcgtgg cctgcctcgc aggccacgag gccgtcacgc tccgacaggc
     3061 ccgcccggca ggtcgggcac acgaacaagc cgagccggtt ccacgtcggg tgtcctccgt
     3121 cgactgcctg caccgttgcc tcccccaaaa tgtcgagctc tccacccggg ccggagcgac
     3181 cctaccggcc cccgtcgcgt cggtcggaac cgctcaactc agcaccccgg tgtgtcgaag
     3241 taccctggat cgggggtcgc cgcggggcgc cacaacgttg gaggggtgcc gtgagccgtg
     3301 gagaaccgtt catccggcgg ggtgtcgtca tcaccctcgt cgccctggtt gcggcgctcg
     3361 tgccgctgtc gacggggatc gcctccgccc agaccgaacc ggcggtcgag cgggccggca
     3421 acctgtcccc ccgcctcgag gacctggcct ccgtcggcgc cgtcgccacc tccgatgccg
     3481 ccgagaaccg cgccatcgac ctccccgcca ccggtgcggg gtccctgctc caccagccgg
     3541 acgggtcctt cctggccaac gcccgcctca cgaccgtgga tgccgccacc gaagcggccc
     3601 tgcagcgcgc cggcgcccgc atcgtcgccc gcagcgcggt cgatcacctg gtcaccgtgg
     3661 ccatcgactc gaccgacatc gagcgggtcg cggccgtggc cggcgtcgag tggctcgaag
     3721 aggtgctcga gccgcgcgtc ggcccggcag acctgcaggg ggtcgcggca tcgctcggcg
     3781 aggacggggt cgccaccaac ggcagctgcg ccgccaaggt cacctccgag ggcgacaccc
     3841 acctcggcgc cgcaacggcc cgcagcacct acggggtcga cggtgccggc gtcaagatcg
     3901 gcgtgctctc ggactcgtac aacaacctgg gcggcgccgc caccgatgtc agcaacggcg
     3961 agctgcccgg agcaggcaac ccgtgcggct acacgacgcc ggtcgtcgtg cagtccgaca
     4021 aggcgagcgg cggcaccgac gaaggccggg ccatggccca agcggtgcac gacctcgcgc
     4081 ccggcgccac gatcatggtg gccacggcgt tcaacggcga cgtcgacttc gcccagcaga
     4141 tccgcaacct ggcgaccgcg ggcgccaagg tgatcgtcga cgacatctcg tacttcaacg
     4201 agcccgtgta ccaggacggg atcatcgcca aagccatcgc ggacgtcacc gccgccggcg
     4261 tgacctactt ctcgtcggcg gcgaactcga acatcatcct cggtggccgc gacgtcgcct
     4321 cctacgaggc cgcgggctac cggcccacga cgtgtcctgg caccgtcacg agccagacgg
     4381 gtgacgccga ctgccacgac tgggacccag gagccggagc cgacggcggc aacctcctca
     4441 ccgtcagcag caacggccag ctgcaggtga agctcggcta cagcgaaccc aagttcggga
     4501 tcacgaccga cctcgacctc ctgctgctcg acaacgccac caacgccatc gtcgcctact
     4561 cggtcagcga caacgtcaac tcgtcgaagg acgcgtacga gctgctcacg tacacgaaca
     4621 gctccggcgc ggcgaagacc taccggctgg tgatcggccg ctacgcaggc gggacgcctc
     4681 gcttcaagac gatcatccag accggcaccg ggttgacggc ggtgcagtgg aacgcctcgt
     4741 cgggcaccga tgtcgtcggg ccgacggcct acggccacaa catgatgcgg ggcgccgggt
     4801 cgatcgccgc catccgctac aactcgacga cggcgcccga gacgttctcg tcgcggggtc
     4861 cggcgaccta ctgctggaac ccggtggtgg ggaccaccgc cgccaccgcg ctggcgtcgt
     4921 gccagtcgga cacgatcgac gtggccgcca ccgacggcgc cgccaactcg ttcttcggcc
     4981 agctctcctc tggtacctgg cgcttctacg gcacgtcgca agcggccccg cacgccgcgg
     5041 ccgtggccgt gctccagctg gcggcgaggc cgtgccgcac ccccgccgag atcctcgccg
     5101 cgcaacgggc ctcaggtctg gccgtcggct cctacggcgt cgatgcggtg ggaggcgggc
     5161 gcgtcgacgc cagcgcggcg atcagcgggc tcgccgcctg ccaagcggtc cccgacgcgc
     5221 cgacagccgt cacgggcacg cccggcaacg gccaggtcac cctgtcgtgg accgctcccg
     5281 cgggctccgg caccaacgcc gtgaccgcct accgcgtcac gcccttcatc ggggcgagcg
     5341 cccaggcccc ggtcgtcttc aactcgaccg ccaccaccca gaccatcacc ggcctcacca
     5401 acggcaccgc ctacacgttc aaggtggcgg ccacgaacgc ctcggggacc ggagcagact
     5461 cggcgccatc agcggctgtc acgccgcgca cggtgccggg agcaccgacc gcgctcacgg
     5521 gctcagccgg cgactcgtcc gcgacgctga actggacggc gcccgcctcc aacggcggct
     5581 cggccgtcac cggctacacc gtcaccccct acatcggcgc caccgcccag
//
LOCUS       DATMFS010000412         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_175324, whole genome shotgun sequence.
ACCESSION   DATMFS010000412 DATMFS010000000
VERSION     DATMFS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175324"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VNQ33_07745"
     CDS             <1..749
                     /locus_tag="VNQ33_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWJ98036.1"
                     /translation="FDVMPNASNTSGVSNALGEGAVDVTETRAVLALQGPAARRLLHA
                     IWPEAAAIGRFHVRRMSWNGIDCVVAGTGYTGEDGLEVAVPAEAATELWDAVLAAGFQ
                     PAGLGARDTLRLEAGLPLHGHELGQGITTLQAGLGWVVSWDKGDFIGRDVLLKEKESG
                     IARRLRALTIEGRRPPRADQIVLVDGTEAGVVTSGNFSPTLEKGIALAFVPPSVEIGG
                     TVQIDVRGEPVDAQVVKPPFVAATPAQPVD"
     gene            762..1217
                     /locus_tag="VNQ33_07750"
     CDS             762..1217
                     /locus_tag="VNQ33_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HWJ98037.1"
                     /translation="MAFRRRRPKEPAPPQIEPLSISASQWRGQQVEAARRLVERYCEA
                     SEMPPSLASLDAAVSGWFDDRAEDRADVNDLVNAVGVTFGHHLAAATGLTWVLATDEH
                     GAELALHRAAGDVLVHPANLVAERIVNGDRGFIQPLHAELAAGIQEISA"
     gene            complement(1240..>2547)
                     /locus_tag="VNQ33_07755"
     CDS             complement(1240..>2547)
                     /locus_tag="VNQ33_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HWJ98038.1"
                     /translation="PEFGQIHPFAPLPQSDGYRQLITELEDWLVEITGYAAVSLQPNA
                     GSQGELAGLLAIRGYHRSRGDEQRNVCLIPSSAHGTNAASAVMAGMRVVVVACDDAGN
                     VDLPDLQAKIAEHGGTLAALMITYPSTHGVFEEAVTDICAAVHDAGGQVYLDGANLNA
                     MVGLARPGRFGADVSHLNLHKTFCIPHGGGGPGIGPVAVGEHLKPFLPNHPLVAEAGP
                     ATGPGPISGGPWGSASILPIPWAYVRMMGGEGLRRATQVAILNANYIAQRLQPHFPVL
                     YTGRGGLVAHECIVDLRPITKETGVSVDDVAKRLIDFGFHAPTMSFPVAGTLMIEPTE
                     SEDLGEIDRFCDAMIAIRAEIAKVGSGEWPLDDNPLANAPHTVNCLTQTGWDHPYTRE
                     EGAFPSPTVRRGDRYFPPVRRIDGAHGDRHLICSCPPVEDFAE"
BASE COUNT          341 a          894 c          956 g          356 t
ORIGIN      
        1 ccttcgacgt catgcccaac gcgtcgaaca cctccggcgt gtcgaacgcg ctgggggagg
       61 gggccgtcga cgtcaccgag acccgcgccg tgctcgccct ccagggcccc gccgcccgtc
      121 gcctgctgca cgccatctgg cccgaggctg ccgccatcgg gcgcttccac gtgcggcgca
      181 tgtcctggaa cggcatcgac tgcgtggtcg ccggcaccgg ctacacgggc gaggacggcc
      241 tggaggtcgc cgtgcccgcc gaggccgcca ccgagctgtg ggacgccgtg ctcgccgccg
      301 ggttccagcc cgccggcctg ggcgcccgag acaccctccg cctggaggcc ggcctcccgc
      361 tccacggcca cgagctgggc cagggcatca ccacgctcca ggccggcctg ggctgggtcg
      421 tgagctggga caagggcgac ttcatcggcc gtgacgtgct cctgaaggag aaggagagcg
      481 gcatcgcgcg ccgcctccgc gccctgacca tcgaaggccg ccgcccgccc cgggccgacc
      541 agatcgtcct cgtcgacggc accgaagccg gcgtcgtcac cagcggcaac ttctccccga
      601 cgctggagaa gggcatcgcc ctcgcgttcg tcccgccatc ggtcgagatc ggcggcaccg
      661 tccagatcga cgtgcgcggc gaaccggtcg acgctcaggt ggtgaagccc ccgttcgtgg
      721 ccgccacccc cgcccagccc gtcgactgaa gcggcggcac gatggcgttc cgccgccgga
      781 gacccaagga accggcaccg cctcagatcg aaccgctctc catctcggcg tcgcagtggc
      841 ggggccagca ggtcgaagcc gcccgccggc tggtcgagcg ctactgcgag gcatctgaga
      901 tgccgccgtc gctcgcctcc ctcgacgccg cggtctcggg ctggttcgac gaccgcgccg
      961 aggaccgagc cgacgtcaac gacctggtga acgccgtcgg cgtcaccttc ggccaccacc
     1021 tggcggcggc caccggcctc acctgggtcc tcgccaccga cgagcacggt gccgagctgg
     1081 ccctccaccg agcggccggc gacgtgctcg tccatcccgc gaacctggtg gccgagcgca
     1141 tcgtcaacgg cgatcgaggc ttcatccagc cgctccacgc cgagctggcg gccggcatcc
     1201 aggagatctc ggcctaggcc cgagaccccc tggccgggtc tactcggcga agtcttcgac
     1261 gggtgggcag ctgcagatga ggtggcggtc gccgtgggcg ccgtcgatgc ggcggacagg
     1321 cgggaagtag cggtcgcccc ggcggaccgt gggggagggg aaggccccct cctcacgggt
     1381 gtaggggtgg tcccacccgg tctgggtgag gcagttgacc gtgtgggggg cgttggccag
     1441 cgggttgtcg tcgagcggcc actcgccgct gccgaccttg gcgatctcgg cgcggatggc
     1501 gatcatggcg tcgcagaagc ggtcgatctc accgaggtcc tcggactcgg tcggctcgat
     1561 catcagcgtg ccggccaccg ggaagctcat ggtgggggcg tggaacccga agtcgatgag
     1621 gcgcttggcg acgtcgtcga ccgacacgcc cgtctccttg gtgatgggtc ggaggtcgac
     1681 gatgcactcg tgggccacca gcccgccccg gcccgtgtag agcacgggga agtggggttg
     1741 gagccgctgg gcgatgtagt tggcgttgag gatggccacc tgggtggcgc ggcgcaggcc
     1801 ctcgccgccc atcatgcgga cgtaggccca cgggatgggc aagatgctgg ccgagcccca
     1861 cggcccaccg gagatgggcc cggggcccgt ggccgggccg gcctcggcca ccagcgggtg
     1921 gttgggcagg aacggcttca ggtgctcgcc caccgcgacc gggccgatgc cggggccgcc
     1981 gccgccgtgg gggatgcaga acgtcttgtg gaggttgagg tggctcacgt cggcgccgaa
     2041 gcgtccgggg cgggccaggc cgaccatggc gttgaggttg gcgccgtcga ggtacacctg
     2101 gccgccggcg tcgtggaccg ccgcgcagat gtcggtgacg gcctcttcga acacgccgtg
     2161 cgtgctcggg taggtgatca tcagcgcggc gagggtgccg ccgtgctcgg cgatcttggc
     2221 ctgcaggtcg ggcagatcga cgttgccggc gtcgtcgcac gccacgacga cgacccgcat
     2281 gccggccatg acggccgagg cggcgttggt gccgtgggcc gacgagggga tgaggcagac
     2341 gttgcgctgc tcgtcgccgc ggctgcggtg gtacccccgg atggccagga ggccggcgag
     2401 ctcgccctgg gagccggcgt tcggctggag gctcaccgcc gcgtagccgg tgatctccac
     2461 gagccagtcc tccagctcgg tgatcagctg gcggtagccg tcggattggg ggagcggggc
     2521 gaaggggtgg atctggccga actcggg
//
LOCUS       DATMFS010000413         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_130899, whole genome shotgun sequence.
ACCESSION   DATMFS010000413 DATMFS010000000
VERSION     DATMFS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130899"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="VNQ33_07760"
     CDS             <1..1642
                     /locus_tag="VNQ33_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWJ98039.1"
                     /translation="ANHVYVSVGHQQMMTDPFTPFGLSLHQRIAGPPMFEAAGRLFVD
                     VTMMLAPPETRPAVIDALGTSDPLIGHALEAVAGRDGFLPDPPAEAPPAPPKIREIPL
                     IPADPAIVATLIERSEASVARLARAIEGRTGTDLLDFIESDVPELKRVLTDPDSRPVI
                     WAGLGAAIWLNEHLLEWLGERSVADALTLSAPGNVTAEMGLALLDVADAVRPHPEVVA
                     YLRSVGGGAPSSGTGVLDGLAALPGGPEARAAIDGFLARHGMRCVGEIDITRPRWSEQ
                     PAALVPAILADVDGFDQGEAARRFERGGAQAEAKAAEVLERLRALPDGKAKAAEAAAM
                     IERVRTFAGYREYPKYGYIRRFGIYKEALLAEADRLVASGVLRASDDAWFLRFEELQE
                     AVRTGTVDHELIAQRREALARYRTLTPPRVLTSEGEAVAGSYRRDDLPAGARAGLGVS
                     SGVVEGRARVIADMAEADIEPGDILVTVGTDPSWSPLFVSIAGLVTEVGGLMTHGAVI
                     AREYGLPAVVGVEHATQLVRDGQRIRIHGTDGYVELLD"
     gene            1926..>2838
                     /locus_tag="VNQ33_07765"
     CDS             1926..>2838
                     /locus_tag="VNQ33_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HWJ98040.1"
                     /translation="MAGGLVGLLDDVAALAKLAAASVDDVGVAAGRASVKAAGVVVDD
                     AAVTPAYVRGLAADRELPIIKEIAVGSLRNKLLFILPVALVLSEIAPTLVEVILLFGG
                     CFLAYDGAHKVIHALKHDDHDHDEPSVEAGPAAEAKTIAGAIRTDFILSAEIMVISLK
                     EVIDEPIVSRAIILALVGILITVVVYGVVALIVKMDDIGLKLAERDSAGSQRTGRMLV
                     AGMPKLLAWLSVIGTAAMLWVGGHIILVGFDELGWEWPYHVVHELEHTVHDWPLHGVL
                     AWIVNTLCSAVVGFAVGFVIAQIVARIP"
BASE COUNT          390 a         1023 c         1014 g          411 t
ORIGIN      
        1 ggcgaaccac gtctacgtgt ccgtcgggca ccagcagatg atgacggacc cgttcacgcc
       61 gttcggcctg tcgctccacc agcggatcgc cggcccgccg atgttcgagg cggcgggtcg
      121 cctgttcgtc gacgtcacca tgatgctggc gccgcccgag acccggccgg ccgtcatcga
      181 cgccctcgga acgtcggacc cgctgatcgg ccacgccctc gaggccgtcg ccgggcgcga
      241 cgggttcctc cccgacccgc cggccgaagc gcctccggct ccgccgaaga tccgggagat
      301 cccgctgatc ccggccgacc ccgccatcgt cgccaccttg atcgagcgca gcgaggcctc
      361 cgtcgcccgg ctcgcccgag ccatcgaggg ccgcaccggc accgacctgc tcgacttcat
      421 cgagtccgac gtccccgagc tcaagcgggt gctcaccgat cccgacagcc gcccggtgat
      481 ctgggcgggc ctgggcgccg cgatctggct caacgagcac ctgctcgagt ggctgggtga
      541 gcggagcgtg gccgacgcgc tgaccctctc cgcacccggc aacgtgaccg ccgagatggg
      601 cttggcgctg ctcgatgtcg ctgacgcggt acgcccgcac cccgaggtcg tcgcctacct
      661 ccgctcggtc ggcggtggtg ccccgagctc cggtaccggg gtcctcgacg ggctggcggc
      721 gctccccggc ggccccgagg cgcgcgccgc gatcgacggg ttcctcgccc ggcacgggat
      781 gcggtgcgtc ggcgagatcg acatcacccg gccccgttgg agcgagcagc ccgcggcgct
      841 cgtccccgcg atcctcgccg acgtcgacgg cttcgaccag ggcgaggcgg cgcggcgctt
      901 cgagcgcggt ggggcgcaag ccgaggccaa ggcggccgag gtgctcgagc ggctccgcgc
      961 cctgcccgac gggaaggcga aggccgccga ggcggcggcg atgatcgagc gcgtccggac
     1021 cttcgccggc taccgcgagt accccaagta cggctacatc cgccgattcg ggatctacaa
     1081 ggaggccctg ctcgccgagg ccgaccgcct cgtcgcgtca ggggtcctcc gggcgagcga
     1141 cgacgcctgg ttcctgcgct tcgaggagct gcaggaagcg gtgcggacgg gcacggtcga
     1201 ccacgagctg atcgcccagc gccgcgaggc gctcgcccgc taccggaccc tcaccccgcc
     1261 gcgggtcctg acctccgagg gcgaggccgt cgccggttcg taccgtcgcg acgacctgcc
     1321 ggcgggggca cgagcggggc tcggcgtctc ctcgggggtg gtcgagggcc gggcccgggt
     1381 catcgccgac atggcggagg ccgacatcga gcccggcgac atcttggtca ccgtcggcac
     1441 cgaccccagc tggtcaccgc tgttcgtgtc gatcgccggt ctggtcaccg aggtcggagg
     1501 cctcatgacc cacggcgccg tcatcgcccg cgagtacggc ctccccgccg tcgtcggtgt
     1561 cgagcacgcg acgcagctcg tccgcgacgg ccagcgcatc cgcatccacg gaaccgacgg
     1621 ctacgtcgag ctcctcgatt gaccgaccgg cggcgggccg ggtcgctggg gcgcgccggg
     1681 taggcgtacg ggaggcggtg ccatgtgccc atggcggccg aacacgcatg gtgagagcgg
     1741 tcttccatca cggagattcc ggaaacatcc gatccaaggc tgaggagcag tggaaagggg
     1801 caggaagggg ggagggacgg gatggttcag gtttctcacc gtgacctgac cggcgggctt
     1861 caggcgcggt ggcggccgag gagcaccgcc tagggtcggc cgaccggacg cacgaggagg
     1921 acgagatggc aggcggcctg gtcgggttgc tcgacgatgt ggcggcgctg gcgaagctgg
     1981 ccgcggcatc ggtcgacgac gtcggcgtcg ctgccggccg ggccagcgtg aaggcggccg
     2041 gcgtggtggt cgacgacgcc gcggtgacgc cggcctacgt gcggggcctg gctgccgatc
     2101 gggagctccc gatcatcaag gagatcgccg tcggctcgtt gcgcaacaag ctgctgttca
     2161 tcctcccggt ggcgctggtg ctgagcgaga tcgcgccgac gctcgtcgag gtcatcttgt
     2221 tgttcggcgg ctgcttcctc gcctacgacg gcgcccacaa ggtcatccac gcgctcaagc
     2281 acgacgacca cgaccacgac gagccgtcgg tcgaagccgg cccggcggcc gaggccaaga
     2341 ccatcgccgg cgccatccgc accgacttca tcctctccgc cgagatcatg gtcatctcgc
     2401 tgaaggaggt gatcgacgag cccatcgtgt cccgggcgat catcctggcg ctcgtcggca
     2461 tcctcatcac cgtcgtcgtg tacggcgtgg tggcgctcat cgtgaagatg gacgacatcg
     2521 gcctcaagct ggccgagcgg gactcggcgg gctcgcagcg gaccggtcgg atgctcgtgg
     2581 ccggcatgcc gaagctgctc gcctggctgt cggtcatcgg cacggcggcg atgctctggg
     2641 tcggcggcca catcatcctc gtgggcttcg acgaactggg ctgggagtgg ccgtaccacg
     2701 tggtccacga gctcgagcac accgtccacg actggccgct gcacggggtc ctcgcctgga
     2761 tcgtcaacac cctctgctcg gccgtcgtcg ggttcgccgt cggcttcgtc atcgcccaga
     2821 tcgtcgcccg catcccca
//
LOCUS       DATMFS010000414         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_340836, whole genome shotgun sequence.
ACCESSION   DATMFS010000414 DATMFS010000000
VERSION     DATMFS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340836"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VNQ33_07770"
     CDS             <1..470
                     /locus_tag="VNQ33_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HWJ98041.1"
                     /translation="AAAISRSGQKTLMFTRTKRMADKLVDQLNEQGVKAAAIHGDLRQ
                     SQREKSLADFMAGKLSVLVATDVAARGIHVDDIDIVIHFDPPEDHKAYLHRSGRTARA
                     GQGGLVVTLLLWDQELEVKRLQKRLGENQPMPEVFSNNPILADLTNWTPVEKV"
     regulatory      754..868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            927..2153
                     /locus_tag="VNQ33_07775"
     CDS             927..2153
                     /locus_tag="VNQ33_07775"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWJ98042.1"
                     /translation="MEEAAGHWSDAASLAVPPWIQPHSSTVPATGAWKPSDPLGQRQF
                     MRMAVDRAFVLEGGGLLNDITICFETWGTLADDASNAVLVCHALTGDAHAAGSAGDGH
                     PTPGWWDDLIGPGRQLDTDRWFVVCVNVLGGCQGSAGPASPMPDRPGCYGPNFPVVSI
                     RDMVRTQARVADALGIARWHAVIGGSMGGMQALEWAVMYPERVGRLVAIATTTAASAQ
                     QIAWWSTGRRAIALDPKWRDGHYYDAAPGDGPHAGLALARQISQVTFRSDDVFTDKFG
                     REPVEPLAGGFEMWQRFEVERYLDYHGIKLARRFDANSYLLLTKAMDLHDLGRGRGGL
                     AAALKRVQAPVLSMGITSDILYPEYQSVDLVAGIEAVGGRAAYHQIDSPHGHDAFLID
                     HDQVAAALVPFLETSS"
     gene            2150..>2446
                     /locus_tag="VNQ33_07780"
     CDS             2150..>2446
                     /locus_tag="VNQ33_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWJ98043.1"
                     /translation="MSNDPTAEPTEPGTGLHPDTVAIRSGRVGDATSLAPVLWPTSTF
                     VSPSVDDARRFATTVGADRFYSRYGNPTVSGFEDAIAQLEGAEAARAYGSGMGAV"
BASE COUNT          358 a          912 c          829 g          347 t
ORIGIN      
        1 tcgctgccgc gatctcccgg agcggtcaga agaccctgat gttcacccgc accaagcgca
       61 tggccgacaa gctggttgac cagctcaacg agcagggcgt gaaggccgcc gccatccacg
      121 gcgacctccg ccagagccag cgcgagaagt cgctggccga cttcatggcc ggcaagctct
      181 cggtcctggt ggcgaccgac gtcgccgccc gcggcatcca cgtcgacgac atcgacatcg
      241 tgatccactt cgacccgccg gaggaccaca aggcgtacct ccaccgctcg ggccgcaccg
      301 cccgcgccgg ccagggcggc ctcgtcgtga ccctgctcct gtgggaccag gagctcgagg
      361 tcaagcgcct ccagaagcgc ctgggcgaga accagccgat gcccgaggtc ttctcgaaca
      421 acccgatcct cgccgacctc accaactgga ccccggtcga gaaggtctga cccagctccg
      481 gcggtggcgt tcggccgtgt cggtcgaaac cccgcccgag atcaccgggc accggcgttc
      541 agacgtgccg ccggggcctg cttctcgaca gggcgaccac cccatcgatc ccacccgcgc
      601 cggcccgtcg ctggcctgag gtggtgcgct cgagtcctct gtgcggaccc cttcggcgtt
      661 tctgacggtg atcgacgacc tggccgagcc ggggtatcgg cgcatgccca tgcagatcgg
      721 ttgtctcggg gtctggcggg tggcgtaggg tggtggagtc atcacgagtg gctcgggaaa
      781 ccgggtccgg caacctggga cggacaccca aggtgcctct cgcttcggcg gggatgtggc
      841 cggttcgccg gcaacgaagt gtccgggccc cggcccgtcg ctggcacctg gtgaccaacc
      901 ccgccaacgc agcgaggagg tcggtcatgg aagaagcagc agggcactgg tccgatgccg
      961 cctcgctcgc cgtgcccccg tggatccagc cgcactccag caccgttccc gccaccggtg
     1021 cgtggaagcc gagcgacccc ctcggccaac ggcagttcat gcgcatggcc gtggaccggg
     1081 ccttcgtgct cgagggcggc ggcttgctca acgacatcac gatctgcttc gagacctggg
     1141 ggacgctcgc cgacgacgcg tcgaacgcgg tcctcgtctg ccacgccctc accggcgacg
     1201 cccacgccgc cggctcggcc ggcgacgggc acccgacgcc gggctggtgg gacgacctga
     1261 tcggaccggg caggcagctc gacaccgatc gctggttcgt cgtgtgcgtc aacgtcctcg
     1321 gcggctgcca gggctccgcg ggtccggcgt ccccgatgcc cgaccggccc ggctgctacg
     1381 gcccgaactt ccccgtcgtg tcgatccgcg acatggtccg cacccaggcc cgggtggccg
     1441 acgccctcgg catcgcccgg tggcacgcgg tgatcggcgg gtccatgggc ggcatgcagg
     1501 cgctggagtg ggcggtcatg taccccgagc gggtcggccg gctggtcgcc atcgccacca
     1561 ccaccgcggc atcggcgcag cagatcgcct ggtggagcac cgggcgacgg gccatcgcgc
     1621 tcgaccccaa gtggcgcgac ggccactact acgacgctgc tccgggcgac ggcccgcacg
     1681 ccggtctcgc cctggcccgc cagatctcgc aggtcacgtt ccgcagcgac gacgtgttca
     1741 ccgacaagtt cggacgagag cccgtggagc cgttggccgg cggcttcgag atgtggcagc
     1801 ggttcgaggt cgagcgctac ctcgactacc acggcatcaa gctggcccgc cggttcgacg
     1861 ccaactccta cctgctgttg accaaggcca tggacctgca cgacctcggg cgggggagag
     1921 gcggcctggc cgcggccctc aaacgggtcc aggcgcccgt cttgtcgatg ggcatcacct
     1981 cggacatcct ctatcccgag taccagtcgg tggacctggt cgcggggatc gaggccgtcg
     2041 ggggtcgggc cgcgtaccac cagatcgaca gcccccacgg gcacgacgcc ttcctcatcg
     2101 accacgacca ggtcgccgcc gccctggtcc cgttcctgga gacatcctcg tgagcaacga
     2161 ccccaccgcc gagccgaccg agcccgggac cgggctgcac cccgacaccg tcgccatccg
     2221 atcgggccgc gtcggcgacg ccacgtcgct cgcccccgtg ctgtggccga cgtcgacgtt
     2281 cgtgtcgccc tcggtcgacg acgcccgccg cttcgcgacg acggtcgggg ccgaccgctt
     2341 ctactcgcgg tacggcaacc cgacggtgag cggcttcgag gatgccatcg cccagctcga
     2401 gggcgccgag gcggcccggg cctacggatc gggcatgggg gcggtg
//
LOCUS       DATMFS010000415         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_317726, whole genome shotgun sequence.
ACCESSION   DATMFS010000415 DATMFS010000000
VERSION     DATMFS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317726"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VNQ33_07785"
     CDS             <1..992
                     /locus_tag="VNQ33_07785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWJ98044.1"
                     /translation="SWSDGAAIEARWRGTDQDGAVAILNQLTWRDADHLAGFAAPADG
                     AVTLVDESEPDQTSYAAELVYTDDAGSVGVGQGVQVQIHTSMADGSTRDVVEAEFLAG
                     RVAGSDGSSSSYDDEFGTLRIAWPGWSAWIDANKTPVTEAQLRAVAATLTPTTEAQMR
                     ALELTTPGGELATEPPVTTTSAAPRPQDPALSLSEAPLQEPITSLEGGETTLAALADG
                     RPMVVTLWATSCVPCIRMVQALDEIARSHPDAVVVAVAEQDAAADVEAFVAQAGVEVP
                     ILLDPDGALLAGWGRSAIPTTVVVGADGRVLGSMTGEASTVSDLEEIITQYLG"
     gene            complement(1123..1980)
                     /locus_tag="VNQ33_07790"
     CDS             complement(1123..1980)
                     /locus_tag="VNQ33_07790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWJ98045.1"
                     /translation="MTGMNLGVTIFLTDLSIGPIELAREVEARGFSSVWLPEHTHIPT
                     SRRTPAPMGEPLDEEYRRCLDPFVTMGMMTAVTDRIRLGTGISLLAERDPIVTAKEVA
                     TLDHVSNGRISLGIGYGWNIEELENHGGSKKTRRAVVRERILAMQELWGTEAAAFHGD
                     HVQMEEAWAWPKPKQQVQGRTGVPVLVGGAPGPILFQHIAEFADGWIPIGGAGVAAAM
                     ADLHAAADEAGRDPSELTVIPFGTLPDQGKLDHYQTLGITETVLRLPSAGRDEVLRVL
                     DSYVPFLDR"
     gene            2073..>2618
                     /locus_tag="VNQ33_07795"
     CDS             2073..>2618
                     /locus_tag="VNQ33_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ98046.1"
                     /translation="MVITGAGRGIGRGHALEFARQGAKVVVNDIGAELDGSGGSTGPA
                     GEVVAEIRASGGQAVANGDDIATWDGGANLVKAAIDAFGGLDVVVNNAGVVRDRMFAN
                     ATEDEWDLTMAVHLKGHFTTSRHAAAYWRDRAKAGETVDARIINTTSGAGLMGSVGQA
                     AYSAAKGGIISLTLVQAAELGR"
BASE COUNT          380 a          915 c          959 g          364 t
ORIGIN      
        1 tgtcgtggtc ggacggtgct gcgatcgagg cccggtggcg ggggaccgac caggacggcg
       61 ccgttgccat cttgaaccag ttgacgtggc gagacgccga ccacctcgcc ggcttcgccg
      121 ccccggcgga cggcgcggtc accctcgtcg acgaatcgga gccggaccag acgagctacg
      181 ccgccgagct ggtctacacg gacgatgccg gatcggtggg cgtgggccaa ggcgtgcagg
      241 tgcagatcca cacctcgatg gccgacgggt ccacccggga cgtcgtggag gccgagttcc
      301 tcgccggccg cgtcgccggc tcggacggat cgagcagctc ctacgacgac gagttcggca
      361 cgctgcgcat cgcctggccg ggttggtcgg cgtggatcga cgccaacaag acgccggtca
      421 ccgaagcgca gctgcgggcg gtcgccgcca ccctcacgcc cacgaccgag gcccagatgc
      481 gggcgctgga gctgacgacg cccggtggcg agctggccac cgaaccaccc gtgacgacca
      541 cgtcggctgc accgcggccg caggacccgg ccctcagcct ctcggaggcc ccgttgcagg
      601 aaccgatcac ctcgctcgag ggcggcgaga cgaccttggc ggcgctcgcc gacggtcggc
      661 cgatggtggt gaccctgtgg gcgacgtcct gcgtgccgtg catccgcatg gtgcaggccc
      721 tcgatgagat cgcccggtcg caccccgacg ccgtggtggt ggcggtcgcg gagcaagacg
      781 ccgcggccga tgtcgaggcg ttcgtcgccc aggccggcgt cgaggtcccg atcctcctcg
      841 atcccgacgg cgcactcctc gccggctggg gtcggtctgc catcccgacc accgtggtgg
      901 tcggtgccga tgggcgggtc ctcggctcga tgaccggtga ggcatcgacc gtgtcggacc
      961 tggaggagat catcacccag tacctcggct gaacatcgcc gagcacccct gagcatcgcg
     1021 gtgcggtcga cccctcccag acgggagggg tcgaccgggt tcccggcccg gagggtgcag
     1081 ccggaccggc cggaaccggc ctcgacgtcg gggccgagac gatcagcgat cgaggaacgg
     1141 cacgtaggag tccaagaccc ggaggacctc gtcgcggccg gcggagggga ggcggaggac
     1201 ggtctcggtg atgccgaggg tctggtagtg gtcgagcttg ccctggtccg ggagggtccc
     1261 gaacgggatc accgtgagct cgctcggatc ccggcccgcc tcgtcggcag cggcgtggag
     1321 gtccgccatg gccgcggcca ccccggcgcc gccgatcggg atccagccgt cggcgaactc
     1381 ggcgatgtgc tggaacagga tcggtcccgg ggcgccgccc accaggacgg gcacgccggt
     1441 gcggccctgg acctgctgtt tgggcttggg ccaggcccag gcctcctcca tctgcacgtg
     1501 gtcgccgtgg aaggcggcag cctcggtgcc ccacagctcc tgcatggcga ggatccgctc
     1561 gcggacgacg gcgcgacggg tcttcttgga gccgccgtgg ttctcgagct cctcgatgtt
     1621 ccagccgtag ccgatgccga ggctgatgcg gccgttcgac acgtgatcga gggtcgccac
     1681 ctccttggcc gtcacgatcg ggtcgcgctc ggcgagcagg ctgatgccgg tgcccaggcg
     1741 gatccggtcg gtgaccgcgg tcatcatccc catcgtcacg aacggatcga ggcagcggcg
     1801 gtactcctcg tcgagcggct cgcccatggg agcgggcgtg cgccggctcg tcgggatgtg
     1861 ggtgtgctcg ggcagccaga cggacgagaa cccccgggcc tccacctccc gcgccagctc
     1921 gatcgggccg atgctgaggt cggtgaggaa gatcgtgacg cccaggttca tgccggtcat
     1981 ggcgcgacag taacctgacg acccgtcaga aacagtttgg gcaacaggac gaacgggtgc
     2041 gatcaatggg tttcctctgc gacggtcgag tggtggtcat cacgggtgcg gggcgcggca
     2101 tcggccgggg ccacgccctc gagttcgccc gccagggggc caaggtcgtg gtgaacgaca
     2161 tcggcgccga gctcgacggc tcgggcggct ccaccggtcc cgcgggcgag gtggtcgccg
     2221 agatccgggc cagcggcggg caggccgtgg cgaacggcga cgacatcgcc acctgggacg
     2281 gcggcgccaa cctggtgaag gctgccatcg acgcgttcgg cggcctcgac gtggtcgtca
     2341 acaacgccgg cgtcgtgcgc gaccgcatgt tcgccaacgc caccgaggac gagtgggacc
     2401 tcaccatggc ggtccacctc aagggccact tcaccacctc ccgccacgcc gccgcctact
     2461 ggcgcgaccg ggccaaagcc ggcgagaccg tggatgcccg catcatcaac accacctccg
     2521 gcgccggcct gatggggtcc gtcggccagg ccgcctactc ggccgccaag ggcgggatca
     2581 tctcgctcac cctcgtgcag gccgccgagc tgggccgc
//
LOCUS       DATMFS010000416        11131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_154183, whole genome shotgun sequence.
ACCESSION   DATMFS010000416 DATMFS010000000
VERSION     DATMFS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154183"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNQ33_07800"
     CDS             <1..302
                     /locus_tag="VNQ33_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HWJ98047.1"
                     /translation="RIDSAEGGWDAPATADWLADAMRAASEATFGQSARSLGEGGSIP
                     FMGMLGRRFPHAQFLLTGVLGPHSNAHGPNEFLHLPTAQKLTAAVALVLDAHATR"
     gene            458..1300
                     /locus_tag="VNQ33_07805"
     CDS             458..1300
                     /locus_tag="VNQ33_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWJ98048.1"
                     /translation="MQLSPWYGDTPIIELDGDPAAISEPTIRQRRRLIDVLAGFDDAR
                     WAHPSRCEGWSNRDVVTHLQSTNQFWGFSIALGLKGEPSQFLATFDPVATPAQLVADA
                     PPVSNAELLDQFRASTEGLAAQLEGLDEAGWRTTAEAPCGHVSISTLAHHALWDSLIH
                     ERDILLPLGVDPVAEPDETLASLRYVAALSPAFAVSRGESPGGILAVDPTDLDAAFRA
                     EAADHVAIRTGPASTAPDLALTGPAVDLLEALSLRAPLDQPVPDAASWFVDGLKVTFD
                     QAPT"
     gene            1449..3224
                     /locus_tag="VNQ33_07810"
     CDS             1449..3224
                     /locus_tag="VNQ33_07810"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ98049.1"
                     /translation="MAEQDRIDEAKARYKAEREKRLRDDAQDQYSQLTDEYEAFDHDP
                     WVEPGFTREPVVTETTVVIVGGGFAGMLTAKGLQERGITDYRIIDKAGDFGGTWYWNR
                     YPGCMCDVESLIYLPLLEETGYTPTMKYAPASEIFEQCQRIGRQYELYPHALFQTEIE
                     ETTWDEAASRWTITTTRDDRISAKFLITAGGILHKAKLPAIEGINDFAGRAFHTARWD
                     YGFTGGSPTEPMDHLKDKVVGIIGTGATAIQVVPQLARSAKEVYVFQRTPSPVGVRAN
                     GPIEPGWVKEQPPGWQAERMANFTECVTGGKPSVNLVGDGATRYLWDDTQQAAATPEE
                     QAELERLDFEVMQAIRDRVDQVVDDPDTAELLKPWYGKHCKRITYHDEYLQSFNRPNV
                     HLVDTAGQGVRQVTAAGPVVDGKEYELDLLVYASGFEVTTGLVSRLGFDPVGVGGVKL
                     SERWHDGAHTLHGVLTNGFPNLLVCHFIQAGFGLNFTHYLQELTAHLTWIVATAEESE
                     IASIEATVEAEDAWLAQLWEAGKGFGRYAAACTPSFHNSEGARTMAAARNVVHPGNLM
                     RYSEHLERWRDAGSVDDLAGVTITR"
     gene            complement(3489..3809)
                     /locus_tag="VNQ33_07815"
     CDS             complement(3489..3809)
                     /locus_tag="VNQ33_07815"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWJ98050.1"
                     /translation="MNPVAIAAVVAVASAITWGLRALPFAALAPLRQSALLAQLRLTM
                     PLGIMIILTIYTVSGIEVTGENLITYGAGIAVTAALQLWKRHVSISILVGTAVHMLLV
                     AWLG"
     gene            complement(3806..4573)
                     /locus_tag="VNQ33_07820"
     CDS             complement(3806..4573)
                     /locus_tag="VNQ33_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWJ98051.1"
                     /translation="MDDPTPEAEPTDPVPTEGHLRMTLPVGLGLFPLGLAFGALVVHA
                     GLSWWWATVFSTIVYAGSLEFLLVGMAVTGVPLAQVALSTLLVNSRHVFYALSFPLHN
                     VDGKVGKAYATFALTDEAYAVTTSPEAQRWTSRQIIGVQVFFQLCWVGGATLGALAGS
                     VLPLDQLDGLDFALTALFVVLAIEAARDRPDRLTAAVAIVCAAVALAISREHLLPIAF
                     AGFTAVQVWRARRDPIAIPPESTGPTAPAAGPGEVPS"
     gene            4661..5596
                     /locus_tag="VNQ33_07825"
     CDS             4661..5596
                     /locus_tag="VNQ33_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWJ98052.1"
                     /translation="MTHDRRFRFGVQTSIASSGEEWAALARQAEDLGYSTMFLPDHFG
                     DQLAPVPAMAAAAAATTDLRVGALVFDNDYKHPVVLAKEMATIDVLSGGRVEVGLGAG
                     WMISDYEQSGIPYDEPRVRVDRFEEGLAIVTGLLGPDPVTFEGTHYQVTAMNGLPKPV
                     QAKPPILIGGGLKRMLTIAGRHADIVGINPTIPNGAVDADAARSGLAGLTDQKLQWVK
                     DAAGDRYADLEINLLNYACIVTDDRAQVANDFAPLFGIAPDDLLEFPHALVGTVDEIC
                     ESIERNRERFDASYIVVQRDAMEAMAPVVARLTGT"
     gene            complement(5613..6350)
                     /locus_tag="VNQ33_07830"
     CDS             complement(5613..6350)
                     /locus_tag="VNQ33_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWJ98053.1"
                     /translation="MATPAPLDPMPRLDDLIAAITQVHPDPLDQLTDAMIAADHLGDL
                     ADHLVGHFVDQARRSGASWTDIGRSMGVTKQAARKRFFPKDPGEPDDLDASQHFNRFT
                     PRARNAVVAAHNEAKEAGNDEITTVHLDIAVLEERGAVGAQAAEAAGLDLDAAVASLR
                     ASLPPAADDRPALVPYDVGAKKALELTFRQALRFGHNYVGTEHLLLALLEADGDDGPL
                     AALGLDPEAVERHIAAALADLTGTEPA"
     gene            6505..7674
                     /locus_tag="VNQ33_07835"
     CDS             6505..7674
                     /locus_tag="VNQ33_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HWJ98054.1"
                     /translation="MPNYGDWQFSIYLNGLTGVRPELPMAYADLERKAEEVMTEQLWS
                     YVSGGAGDERTQRANIRAFDRWGLMPRMLAGAAQRNLSIELFGQTLPTPLLMAPVGVI
                     GIVDPDGHGDLVTARAAASSGVPMVASTLMMDPMEEVAAAFGDTPGWFQLYPPNDREL
                     TESLVHRAEAAGFSAIVVTLDTLTLGWRPRDLTIASFPQLQGLCLANYFSDPVFRSRL
                     AAPPEEDVQAATGHWAMTFGNQSLGWDDLAWLRSLTDLPLLLKGICHPDDARQAVDLG
                     IDGIYCSTHGGRQANGGLPALDCLPGIVEAAGDKPVIFDSGIRSGPDVVKALALGATA
                     VGVGRPYTYGLAVGGQAGIEHVLSCLLAEIDITLAIDGYPNVSQLTPDALRRVGG"
     gene            7734..8489
                     /locus_tag="VNQ33_07840"
     CDS             7734..8489
                     /locus_tag="VNQ33_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWJ98055.1"
                     /translation="MGSLLLVALAGAACGVVNSIAGGGSLILFPTLLLTGMSPLSANV
                     TNSVATWPGYLGGVGAFRAEIGTRKHQLPWLLGATLAGSATGCVLLLVTPEDSFDVVV
                     PWLVLVATVMTALQPVVRKRLEDQDTADARPPMSAVTAIFLATIYGGYFGAALGVIVL
                     AALSLTLNDTIKNLNATKSVISLVDASVSVVVFGLFGPVEWQYVLVAAPTTLLGGYAG
                     GRIARRIDEDLLRTLVVALGGTVTVLLFVRAFA"
     gene            complement(8500..10104)
                     /locus_tag="VNQ33_07845"
     CDS             complement(8500..10104)
                     /locus_tag="VNQ33_07845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ98056.1"
                     /translation="MSETAESTNGLAWIPAAALRLRNGVVVEVNRAAQDVLGPPEQVL
                     ARFDPADRTRLRALAEAAGRPGAEPGGPVMFRLGQEAPHRFVELELGPHPGGGAIAVA
                     HDVTERERLDAVIAEFATGVYITDADLEATWIPRRVSESAGLPRDQFVGSDVYSMLHP
                     DDVAPTRALVARARETPGVRCSRPLRVHQIGQPDVWWPIIVHVVWRGDDPAIGGLLVR
                     FDMDLTAGVHLQGSDATAQALVTLGSSSITGALHISKSGALLQRSTRVREILRPVGED
                     GDLRWLDLLRPEHVDAVTERLAAARADSPLPAVEVAFAGNGAEVLARVEVVPYRDVQG
                     HVAGMFASVTDLSAEREARDELAAAREELWHLANHDALTGLANRYQLADRLAAAIPGT
                     GAADGAAPVLPGLIVADLDRFKAVNDGHGHRIGDAVLMEAARRIAAAVRPADLACRFG
                     GDEFVVLCEQIASPGELDAIADQIVARFREPFDIEGVVFSIGVSVGGALAVPDDADDP
                     EDLILRADRAMYAAKARRRRAPSGES"
     gene            complement(10272..10940)
                     /locus_tag="VNQ33_07850"
     CDS             complement(10272..10940)
                     /locus_tag="VNQ33_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ98057.1"
                     /translation="MGGLELGSSVGRGPGGAEATQEGGEPGHAGNVRNGCCIRSSPAF
                     THGDHPLTICCRSVRASARFRLRTESRHLGSAPMPVTVEIADDVTDELVEAFVRLTPQ
                     LSSSSPPPDRDQLAEIVASPATHLLIARGDDGTILGSLTLVLFRIPTGLRAWIEDVVV
                     DGGARGQGAGEALNRFAIDVAAERGARSVDLTSRPSREAANRLYRRLGFEPRETNVYR
                     YRGS"
     gene            10857..11039
                     /locus_tag="VNQ33_07855"
     CDS             10857..11039
                     /locus_tag="VNQ33_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98058.1"
                     /translation="MTWLPSLLRGLGTAGATANARAELETAHTRNLQAAIVAQRVNRS
                     GHAGRPLPPVAEARVA"
BASE COUNT         1611 a         4180 c         3838 g         1502 t
ORIGIN      
        1 tccgcatcga cagcgccgag ggcggctggg atgcaccggc cacagccgac tggctggccg
       61 acgcgatgcg cgccgccagc gaggcaacgt tcggccagtc cgcccggagc ctcggcgagg
      121 gcggctcgat cccgttcatg ggcatgttgg gccgccgctt cccccacgcc cagttcctgc
      181 tcaccggcgt cttgggcccc cactccaacg cccacggacc caacgagttc ctccacctcc
      241 cgacggccca gaagctgacc gccgcggtcg ccctcgtcct cgacgcccac gccacccgct
      301 gaccggaccg gtcgggaccg cccggtcggg gcggtcggcg ccatcaggcg atgcgtcgcc
      361 ccgaccaccg tcgtggcgac cagatgggca cgggccaggg acgggacagg ccgatcctcc
      421 tgccactccg tctccggtcc tgtgacagag tgccgtcatg caactcagtc cgtggtacgg
      481 cgacaccccg atcatcgagc tcgacggcga ccccgcggcc atcagcgagc cgaccatccg
      541 ccagcgccgc cggctgatcg acgtgctggc ggggttcgac gatgcgcggt gggctcatcc
      601 gagccgctgc gagggctggt ccaaccgcga cgtcgtcacg cacctccaga gcacgaacca
      661 gttctggggc ttctcgatcg ccctgggcct caagggcgag ccgtcgcagt tcctcgccac
      721 gttcgacccg gtcgccacgc cggcccagct ggtggccgac gccccaccgg tgtccaacgc
      781 cgagctgctc gaccagttcc gtgcctctac cgagggcctg gccgctcagc tcgaggggct
      841 cgacgaggcc ggttggcgga cgacggccga ggcgccgtgc ggccacgtga gcatcagcac
      901 cctcgcccac cacgccctct gggactcgct gatccacgag cgcgacatcc tcctccccct
      961 cggcgtcgac ccggtggcgg agcctgacga gacgctggcc tcgctgcgct acgtcgccgc
     1021 cctcagcccg gcgttcgcgg tgagccgcgg cgagtcaccc ggcggcatcc tggccgtcga
     1081 tcccaccgac ctggacgccg cgttccgcgc cgaggccgcc gaccacgtcg ccatccgcac
     1141 cggcccggca tccaccgcgc ctgacctggc cctcaccggc cccgccgtcg acctgctcga
     1201 ggcgctcagc ctccgcgccc cgctcgacca gccggtaccg gatgcggcct cgtggttcgt
     1261 cgacggcctc aaggtcacct tcgaccaagc ccccacctga cggcctcccc cgtccgtcct
     1321 caccacgaga agcgcccctc cgcgagcgcg gatcgcgacg agatccgatg cggccgatgt
     1381 ggaccggcgg cgctggaccg gccagactgg aaaagtagaa cacgttctag aaacgggtgg
     1441 caggacgcat ggctgagcag gaccgcatcg acgaggcgaa ggcgcgctac aaggccgagc
     1501 gcgagaagcg gctgcgcgac gacgcccagg accagtactc gcagctgacc gacgagtacg
     1561 aggcgttcga ccacgacccg tgggtcgagc ccggcttcac ccgcgagccg gtggtcaccg
     1621 agaccacggt cgtcatcgtc ggcggcggct tcgccgggat gctcaccgcc aagggcctcc
     1681 aggagcgggg catcaccgac taccggatca tcgacaaggc cggcgacttc ggcggcacct
     1741 ggtactggaa ccgctacccc ggctgcatgt gcgacgtgga gtcgctcatc tacctgccgc
     1801 tcctcgagga gaccggctac acgccgacga tgaagtacgc accggcgagc gagatcttcg
     1861 agcagtgcca gcgcatcggg cgccagtacg agctctaccc tcacgccctc ttccagacgg
     1921 agatcgaaga gacgacctgg gacgaggctg cctcccgctg gaccatcacc accacccgcg
     1981 acgaccggat ctcggccaag ttcctgatca ccgccggcgg catcctccac aaggccaagc
     2041 tcccggccat cgagggcatc aacgacttcg ccggccgggc cttccacacg gcccgctggg
     2101 actacggctt caccggcggc agccccaccg agccgatgga ccacctcaag gacaaggtcg
     2161 tcggcatcat cggcaccggc gccaccgcca tccaggtcgt gccgcagctg gcgaggtcgg
     2221 ccaaggaggt ctacgtcttc cagcgcacac cgtcaccggt cggcgtgcgg gccaacgggc
     2281 cgatcgagcc gggttgggtc aaagagcagc ccccggggtg gcaggccgag cgcatggcca
     2341 acttcaccga gtgcgtcacc ggcggcaagc cgtcggtcaa cctcgtgggc gacggcgcca
     2401 cccgctacct ctgggacgac acccagcagg cagcggccac gcccgaagag caggccgagc
     2461 tggagcgcct cgacttcgag gtcatgcagg cgatccgcga ccgggtcgac caggtcgtcg
     2521 acgaccccga caccgccgag ctgctgaagc cctggtacgg caagcactgc aagcggatca
     2581 cgtaccacga cgagtacctc cagtcgttca accggccgaa cgtccacctg gtcgacaccg
     2641 ccggccaggg cgtccgccag gtgaccgccg ccggccccgt ggtcgacggc aaggagtacg
     2701 agctggacct gctcgtgtac gcctcgggct tcgaggtcac gaccgggctg gtgagccggc
     2761 tcgggttcga tccggtcggc gtcggcggcg tgaagctgag cgagcgctgg cacgacggcg
     2821 cccacacgct ccacggcgtg ctcaccaacg gcttcccgaa cctgctggtg tgccacttca
     2881 tccaggccgg gttcggcctc aacttcaccc actacctcca ggagctgacc gcccacctca
     2941 cgtggatcgt cgcgaccgcc gaggagtccg agatcgcgtc gatcgaggcc accgtcgagg
     3001 ccgaggacgc ctggctggcc cagttgtggg aggccggcaa gggcttcggc cgctacgccg
     3061 ccgcctgcac gccgagcttc cacaacagcg agggcgcccg cacgatggcg gcggcccgca
     3121 acgtcgtcca ccccggcaac ctcatgcgct actccgagca cctcgagcgc tggcgcgacg
     3181 ccggctcggt cgacgacctc gccggcgtca ccatcacccg ctgaccgcga ccctcccctc
     3241 ccctccccac accccctccc ctcccctccc ctcccctccc cactcctcca ctcctccact
     3301 cctccacgaa cttcgtatgc gagcgctccc atttgggcgt tctgccctac gaagctcgcc
     3361 caggccggcc aaaccctcac gaacttcgta ggggagagcg ccgctctggg cgctctgaca
     3421 tacgaagttc gattcggtgg ggtggggtcg attcggtggg gtggggtcgc ctggatcggt
     3481 ggggagggtc agccgagcca ggccaccagg agcatgtgga ccgccgtgcc cacgaggatg
     3541 ctgatgctga cgtggcgctt ccagagctgg agggcggccg tcacggcgat gccggcgccg
     3601 taggtgatga ggttctcgcc ggtcacctcg atccccgaca ccgtgtagat ggtgaggatg
     3661 atcatgatgc cgagcggcat cgtcagccgg agctgggcga gcagcgcgct ctggcgcagc
     3721 ggggcgaggg cggcgaacgg cagggcgcgc agcccccacg tgatggccga ggcgacggcc
     3781 acgacggcgg cgatggcgac cgggttcacg acggcacctc gcctggtccc gccgcgggcg
     3841 ccgtcgggcc ggtcgactcg ggcgggatgg cgatggggtc ccgccgggct cgccacacct
     3901 ggacggccgt gaagccggcg aaggcgatcg gcaacaggtg ctcccgcgag atggcgaggg
     3961 cgacggcggc gcacacgatg gcgacggctg ccgtcagccg atcgggccgg tcgcgggcgg
     4021 cctcgatggc cagcaccacg aagagggcgg tgagggcgaa gtcgaggccg tcgagctggt
     4081 cgagcggcag caccgacccg gccagcgcac cgagcgtggc tccgcccacc cagcacagct
     4141 ggaagaagac ctgcaccccg atgatctggc gcgaggtcca gcgctgggcc tccggcgacg
     4201 tggtgacggc gtaggcctcg tcggtgaggg cgaacgtggc gtacgccttg cccaccttcc
     4261 catcgacgtt gtggagcggg aacgacagcg cgtagaagac gtggcgcgag ttcacgagca
     4321 gggtcgacag cgccacctgg gcgagcggta ccccggtgac ggccatgccc accagcaaga
     4381 actcgagcga gccggcgtag acgatcgtcg agaacaccgt cgcccaccac cacgacagcc
     4441 cggcgtggac gacgagggcg ccgaatgcca gacccagcgg gaacagcccg aggcccaccg
     4501 gcagggtcat gcgcaggtgg ccctcggtcg gcaccggatc ggtgggctcc gcctccggcg
     4561 tcgggtcgtc catcgccgcc gatcatgccc cgccgccgcc cgacggccac cagatttcgt
     4621 tcagctccgc ctcgcagacg gcggcggtat ggtcccggcc atgacgcacg accgccgctt
     4681 ccgcttcgga gtccagacct cgatcgcctc gtcgggggag gagtgggccg ccctcgcccg
     4741 ccaggccgaa gacctcgggt actcgacgat gttcctgccc gaccacttcg gcgatcagct
     4801 cgccccggtg cccgccatgg ccgcggcggc cgcggccacc accgacctgc gcgtcggtgc
     4861 gctcgtgttc gacaacgact acaagcaccc cgtcgtcctg gccaaggaga tggccaccat
     4921 cgacgtgctg tcgggcggcc gggtcgaggt cggcctcggc gccggctgga tgatcagcga
     4981 ctacgagcag tccggcatcc cctacgacga gccccgggtc cgtgtcgacc gcttcgagga
     5041 ggggctggcg atcgtcacgg gcctgctcgg ccccgacccg gtcaccttcg agggcaccca
     5101 ctaccaggtc acggcgatga acggcctgcc caagccggtc caggccaagc ctccgatcct
     5161 catcggcggc ggcctcaagc ggatgctgac catcgccggc cgccacgccg acatcgtggg
     5221 catcaacccc accatcccca acggcgccgt cgacgccgat gccgcccgct cgggcctggc
     5281 cggcctcacc gaccagaagc tccagtgggt caaggacgcg gcgggcgacc gctacgccga
     5341 cctcgagatc aacctcctca actacgcctg catcgtcacc gacgaccggg cccaggtcgc
     5401 caacgacttc gctcccctgt tcggcatcgc gcccgacgac ctgctcgagt tcccgcacgc
     5461 gctggtgggc acggtcgacg agatctgcga gtccatcgaa cgcaaccgcg agcgcttcga
     5521 cgcctcgtac atcgtcgtcc agcgcgacgc catggaggcc atggcccccg tcgtcgcccg
     5581 cctcaccggg acctgatccc tgatcgggac cgtcaggcgg gctcggtgcc cgtgaggtcg
     5641 gcgagcgcgg cggcgatgtg gcgctcgacg gcctcggggt caagccccag cgccgccagc
     5701 ggcccgtcgt cgccatcggc ctcgagcagc gccagcagca ggtgctccgt gccgacgtag
     5761 ttgtggccga accgcagcgc ctggcggaac gtgagctcca acgccttctt cgcccccacg
     5821 tcgtagggca ccagcgcggg ccggtcgtcg gcggcgggag ggaggctggc gcgcagcgag
     5881 gcgacggccg cgtcgaggtc gaggccggcc gcctcggctg cctgggcgcc gacggcgccg
     5941 cgctcctcga gcaccgcgat gtcgaggtgc acggtcgtga tctcgtcgtt gccggcctcc
     6001 ttggcctcgt tgtgcgccgc gacgacggcg ttgcgtgccc gaggcgtgaa ccggttgaag
     6061 tgctggctgg catcgaggtc gtcgggctcg cccgggtcct tggggaagaa gcgcttgcgg
     6121 gccgcctgct tggtgacgcc catgctgcgg ccgatgtcgg tccacgaggc gccggatcgg
     6181 cgggcttggt ccacgaagtg ccccacgagg tggtcggcca ggtcgcccag gtggtcggcg
     6241 gcgatcatgg cgtcggtgag ctggtcgagc gggtcggggt ggacctgggt gatcgcggcg
     6301 atgaggtcat cgagccgggg catcgggtcg agcggagcgg gtgtggccat ggcgacaacc
     6361 ataggttgtc gatccctgat cgtcaaccca gggttgtcgg tggtgcgggt gctccgccca
     6421 ggagcacccg cgcccgctca ccacgacgca cggccggacg gaccgcatct aggttctggc
     6481 cagcgaccca ccctggaggc gacgatgccg aactacggcg actggcagtt ctcgatctac
     6541 ctgaacgggc tcaccggggt gcgacccgag ctcccgatgg cctacgccga cctcgagcgg
     6601 aaggccgagg aggtcatgac cgagcagctg tggtcctacg tgtccggcgg ggccggcgac
     6661 gagcgcaccc agcgggccaa catccgcgcg ttcgaccgct ggggcctgat gccccgcatg
     6721 ctggccggtg ccgcccagcg aaacctcagc atcgagctgt tcggccagac cctgccgacg
     6781 ccgctgctga tggcgcccgt cggggtgatc ggcatcgtcg accccgacgg ccacggcgac
     6841 ctcgtcaccg cccgagccgc ggcgtcgagc ggcgtgccga tggtggcgtc gacgctgatg
     6901 atggacccga tggaggaggt ggccgccgcg ttcggcgaca ccccgggctg gttccagctc
     6961 tacccgccga acgaccggga gctgaccgag agcctcgtgc accgggccga agccgccggc
     7021 ttctcggcca tcgtcgtcac gctcgacacg ctcaccctcg ggtggaggcc ccgagacctc
     7081 accatcgcca gcttcccgca gctgcagggc ctgtgcctgg ccaactactt ctccgatccc
     7141 gtcttccgat cccgcctcgc ggccccgccc gaggaggacg tgcaggccgc caccggccac
     7201 tgggcgatga cgttcggcaa ccagtccctc ggctgggacg acctcgcctg gctgcgctcc
     7261 ctcaccgacc tccccctgct gctcaagggc atctgccacc ccgacgacgc ccgccaggcg
     7321 gtcgacctcg gcatcgacgg gatctactgc tcgacgcacg gcggccggca ggccaacggc
     7381 ggtctgccgg cgctcgactg cctgcccggc atcgtcgagg cggccggcga caagccggtc
     7441 atcttcgact cgggcatccg cagcgggccc gacgtggtga aggccctcgc cctcggagcg
     7501 acggcggtcg gcgtcggccg gccctacacc tacgggctcg cggtcggcgg ccaggccggc
     7561 atcgagcacg tgctgagctg cctgctcgcc gagatcgaca tcaccctcgc catcgacggg
     7621 tacccgaacg tgtcgcagct cacgcccgac gccctgcgcc gggtcggcgg gtgacggcgg
     7681 acaccgggcg agcgccttcg cctcgccggc cggccgcggg ctagaccgca gcggtgggat
     7741 cgctcctcct ggtcgcgctc gcgggtgcgg cctgcggcgt cgtcaacagc atcgccggcg
     7801 gcggctcgct gatcctgttc cccaccctcc tgctcacggg gatgtcgccg ctgtcggcca
     7861 acgtcaccaa ctcggtggcc acgtggccgg gctacctcgg cggcgtcggg gcgttccggg
     7921 ccgagatcgg cacccgcaag caccagctcc cctggctcct gggcgccacg ctcgccgggt
     7981 ccgccaccgg ctgcgtgctg ctcctggtga cccctgagga ctcgttcgac gtcgtcgtgc
     8041 cctggctggt gctggtggcc acggtgatga ccgcgctgca acccgtggtg cgcaagcggc
     8101 tcgaggacca agacaccgcc gatgcccgtc cgccgatgtc cgccgtcacc gcgatcttcc
     8161 tggccaccat ctacggcggc tacttcggtg cggcgctcgg cgtcatcgtc ctcgcggccc
     8221 tgtcgctcac gctcaacgac acgatcaaga acctcaacgc caccaagtcg gtcatctcgc
     8281 tggtcgacgc ctccgtgagc gtggtggtgt tcggcctgtt cggcccggtc gagtggcagt
     8341 acgtcctcgt cgccgccccc acgaccctgc tcggcggcta cgccggcggc cgcatcgccc
     8401 ggcggatcga cgaggacctg ctccgcaccc tggtcgtcgc cctcggcggc accgtgacgg
     8461 tgctcctctt cgtccgcgcc ttcgcctgac tcgggcgcgt cacgactccc cgctgggagc
     8521 gcggcggcgc ctcgccttcg cggcgtacat cgcccggtcg gcccgcagga tcaggtcctc
     8581 ggggtcgtcc gcatcgtcgg gcaccgcgag cgcaccgccc acgctgacgc cgatcgagaa
     8641 caccacgccc tcgatgtcga agggctcgcg gaaccgggcg acgatctggt cggcgatcgc
     8701 gtcgagctcg ccggggctgg cgatctgctc gcacagcacc acgaactcgt cgccaccgaa
     8761 ccggcaagcc aggtcggcgg gtcgcacggc ggcggcgatg cggcgggcgg cctccatgag
     8821 cacggcgtcg ccgatgcggt gcccgtggcc gtcgttgacc gccttgaagc ggtcgaggtc
     8881 ggcgacgatc agccccggca gcacgggcgc ggcgccatcg gccgcgcccg tccccgggat
     8941 cgccgccgcc aggcggtcgg ccagctggta gcggttggcc aggccggtga gggcgtcgtg
     9001 gttggcgagg tgccacagct cctcgcgagc cgcggccagc tcgtctcgcg cctcgcgctc
     9061 ggcggacagg tcggtgacgc tggcgaacat gccggccacg tgcccctgca cgtcccggta
     9121 cgggaccacc tcgacgcggg cgaggacctc ggcgccgttg ccggcgaacg ccacctcgac
     9181 ggcgggcagc ggcgagtcgg cccgagccgc ggcgaggcgc tcggtcacgg cgtcgacgtg
     9241 ctcgggccgc agcaggtcga gccagcgcag gtcgccgtcc tcgccgaccg gccgcaggat
     9301 ctcgcggacc cgcgtcgagc gctggaggag cgcgcccgac ttggagatgt gcagcgcgcc
     9361 ggtgatcgac gacgatccca gggtgacgag cgcctgggcc gtggcatcgc tgccctgcag
     9421 gtggacgccg gcggtgaggt ccatgtcgaa gcgcaccagc agcccgccga tggccgggtc
     9481 gtcgccccgc cagacgacgt gcacgatgat cggccaccag acgtcgggct ggccgatctg
     9541 gtggacccgc agcggacgcg agcagcggac gcccggcgtc tcccgcgccc gggccacgag
     9601 ggcccgggtg ggggccacgt cgtcgggatg gagcatcgag tagacgtcgc tccccacgaa
     9661 ctggtcccgc gggagtccgg ccgactcgct gacgcggcgc gggatccacg tcgcctcgag
     9721 gtcggcgtcg gtgatgtaca cgcccgtggc gaactcggcg atcacggcgt cgagccgctc
     9781 gcgctcggtc acgtcgtgcg ccaccgcgat ggccccgcct ccggggtgcg ggccgagctc
     9841 gagctcgacg aaccggtgcg gcgcctcctg cccgagccgg aacatcaccg ggccgccggg
     9901 ctcggcaccg ggacgcccgg ccgcttccgc cagtgcccgc aaccgggtcc ggtctgccgg
     9961 gtcgaaccgg gccagcacct gctccggcgg accgagcacg tcctgggccg ctcggttcac
    10021 ctcgacgacc acgccgttgc gcaggcgcag cgcagccgcg gggatccagg ccagtccgtt
    10081 ggtgctctcc gccgtttcac tcatcctctg cgcagcgtac ccggccctcc cccacggtga
    10141 acgggccagg ttgcccagtc ggacgcgacg ggtggcggcc cgcccagcgc gtcggtgcac
    10201 cctcgaccag ctggcgccat cgaggtctcg gccaccccgc cccgaccggg cacgggccgg
    10261 gcagcccgag gtcaggaccc gcggtagcgg tagacgttgg tctctcgcgg ctcgaagccg
    10321 aggcggcggt agaggcggtt ggcggcttct cggctcgggc gggaggtgag gtcgaccgag
    10381 cgggcgccgc gctcggcggc gacgtcgatg gcgaagcggt tgagcgcctc gccggcacct
    10441 tggccccgcg cgccgccgtc gaccaccacg tcttcgatcc aggcccggag cccggtcggg
    10501 atgcggaaga gcacgagggt gagcgacccg aggatcgtgc cgtcgtcgcc gcgggcgatc
    10561 agcaggtggg tggcgggcga cgccacgatc tccgcgagct ggtcccgatc cggcgggggg
    10621 gacgaggagg agagctgcgg ggtgaggcgg acgaaggcct cgaccagctc gtcggtgacg
    10681 tcatcggcga tctctacggt gacaggcatc ggcgccgacc ctagatgccg gctctcggtc
    10741 cgaagccgga accgagcaga agctcggacc gatcgacagc aaatcgtcag cggatggtca
    10801 ccgtgcgtga acgcagggga cgaccggatg caacacccgt ttcgtacgtt cccggcatga
    10861 cctggctccc ctccctcctg cgtggcctcg gcaccgccgg ggccacggcc aacgctcgag
    10921 ccgagctcga gaccgcccac acccgcaacc tgcaggccgc gatcgtggcc cagcgggtca
    10981 accgctccgg acacgccggc cgtcccctgc cgccggtcgc cgaagcacgg gtcgcctagc
    11041 ggcccactgg tcgagcgccg ggtcccggat gtcccccttg ggcccggcgc tgaccagcac
    11101 cacgacccgg atccctgggt caccccggtg g
//
LOCUS       DATMFS010000417         3965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_14584, whole genome shotgun sequence.
ACCESSION   DATMFS010000417 DATMFS010000000
VERSION     DATMFS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14584"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VNQ33_07860"
     CDS             <1..568
                     /locus_tag="VNQ33_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HWJ98059.1"
                     /translation="DCYTIRQNRGDLRGQHIAIVGDIKHSRVARSDVLAFTAMGAEVT
                     LVAPPTLLPPSLEGWPVKVSYDLDQVLPTVDVVYLLRMQRERMSEALLPSLREYTATY
                     GLTNRRVDLLADDALVMHPGPMNRGVEIAAEVADLPRSVILDQVRNGVAVRMAVLYLL
                     LGSGTGLVAAPADPTPATDPIEEEPARA"
     gene            561..1844
                     /locus_tag="VNQ33_07865"
     CDS             561..1844
                     /locus_tag="VNQ33_07865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWJ98060.1"
                     /translation="MPESPSIVVKGGRVVDATGERTADVVIAGGRIAAVGPDLDADRT
                     LDAAGCIVAPGLVDIHTHLREPGKEEAETVETGARGAALGGYTAILAMPNTTPAIDSA
                     AVVQQVLDLGRTAPCDVYTSGAITVGRAGEQLAPMAEMAALGVRIFTDDGAGVQSNAM
                     MRRAMEYAQGLGVTLAQHCEDDALCGGGHMHEGEWSARLGIPGQPAEAEELMVMRDIA
                     LARLTGCRIHFQHMSTARSVAMIRDAIAAGLPVTAEATTHHFTLTHAECASYDPLYKV
                     NPPLRTADDVEAVKAGLADGAIGIIATDHAPHTAETKELPFDQAPPGMLGLETALALA
                     LTELDLPIQQVLAALSWNPAAVIGAGDTHGLPVAEGNPANLCVIDPEATWTVDGAAMA
                     SRSHNTPYQGRELTGRVRHTILAGEPVVIGGEAQR"
     gene            1903..2151
                     /locus_tag="VNQ33_07870"
     CDS             1903..2151
                     /locus_tag="VNQ33_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWJ98061.1"
                     /translation="MALSIKSDEADRLARELVQLTGETLTDAVTEALRERVQRKRPEP
                     KDPHAVLARLQAEFAANRTDDKRTDDEILGYDEFGLPS"
     gene            2148..2540
                     /locus_tag="VNQ33_07875"
     CDS             2148..2540
                     /locus_tag="VNQ33_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ98062.1"
                     /translation="MIVDSSAVVAMLAREPDAAALEAALARADEPVIGAATFVEASTV
                     VAARMHDGARAVRELLESFGVEVVAIDEAGAAAAVDGWRRFGRGNHRARLNFGDCLTY
                     GVAAVRGEPILCIGNDFVQTDATVVPLG"
     gene            complement(2544..3353)
                     /locus_tag="VNQ33_07880"
     CDS             complement(2544..3353)
                     /locus_tag="VNQ33_07880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98063.1"
                     /translation="MALPTPAPDRTAVVTGASSGIGAAIARDLASRGHGVTLVARSAD
                     KLADLAAELRGSGVRAEVLATDLTDRAARAGLPDRVTGLGLVPDIFVNNAGLSTLGRV
                     SAADPDAEMAMIELDVVAVVDLTVRFLPGMVERGRGGILNVASTAAFQPLPGQAGYGG
                     CKAFVLSYTRSLSGELKGTGVHATALCPGPVDTGFGEAAGFDKDEAEASLPSFMWEPP
                     EAVAKAGLDALAANHPVAIPGTANRIGATFAHLAPKRLLVPLLASRHPGLK"
     gene            3563..>3965
                     /locus_tag="VNQ33_07885"
     CDS             3563..>3965
                     /locus_tag="VNQ33_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HWJ98064.1"
                     /translation="MTVRDALLVLTDGTVFEGEALGATPASGIATGEVVFNTVLSGYQ
                     EVLSDPSYAGQIITFTYPHIGNYGVTAADDESRTPFCRGVVVRELARRRSNWRATEDL
                     DEWLARHGVPGIGGVDTRRLTRHIRDTGAMPG"
BASE COUNT          565 a         1457 c         1408 g          535 t
ORIGIN      
        1 cgactgctac acgatccgcc agaaccgcgg cgacctgcgc ggccagcaca tcgccatcgt
       61 cggcgacatc aagcacagcc gggtggcccg ctccgacgtc ctcgccttca cggcgatggg
      121 cgccgaggtg acgctggtgg cgccgccgac gctgctcccg ccgagcctcg agggctggcc
      181 ggtgaaggtg agctacgacc tggaccaggt gctgcccacc gtcgacgtcg tctacctgct
      241 ccgcatgcag cgcgagcgca tgagcgaggc gctgctcccg tcgctgcggg agtacaccgc
      301 cacctacggc ctcaccaacc ggcgggtcga cctgctggcc gacgacgccc tggtgatgca
      361 ccccggcccc atgaaccgcg gtgtggagat cgccgccgag gtcgcggacc tgccccggtc
      421 cgtgatcctc gaccaggtcc gcaacggcgt cgccgtgcgc atggccgtcc tgtacctgct
      481 cctcggctcc ggcaccggcc tcgtggccgc cccggccgac ccgacccccg ccaccgaccc
      541 catcgaggaa gaacccgccc gtgcctgagt ccccgtccat cgtcgtcaag ggcgggcgcg
      601 tcgtcgacgc caccggcgag cgcaccgccg acgtcgtcat cgccggcggc cgcatcgctg
      661 ccgtcggccc cgacctcgat gccgaccgca ccctcgacgc cgccggctgc atcgtcgccc
      721 ccggcctggt cgacatccac acccacctgc gcgagccggg caaggaggag gccgagaccg
      781 tcgagaccgg tgcccggggt gccgccctcg gcggctacac cgccatcctc gccatgccca
      841 acaccacgcc ggccatcgac agcgccgcgg tcgtgcagca ggtgctggac ctcggccgga
      901 cggcgccgtg cgacgtctac acgtcgggcg ccatcaccgt cggccgggcg ggggagcagc
      961 tggcccccat ggccgagatg gccgcgctcg gcgtgcgcat cttcaccgac gacggcgccg
     1021 gcgtgcagag caacgccatg atgcgccggg cgatggagta cgcccagggc ctgggcgtca
     1081 ccttggccca gcactgcgag gacgacgccc tgtgcggtgg cggccacatg cacgaagggg
     1141 agtggtcggc gcgcctcggc atccccggcc agcccgccga ggccgaggag ctgatggtga
     1201 tgcgcgacat cgccctggcc cgcctcaccg gctgccgcat ccacttccag cacatgagca
     1261 ccgcccggtc cgtcgccatg atccgcgacg ccatcgccgc cggcctgccg gtcacggccg
     1321 aggcgaccac ccaccacttc acgctcaccc acgccgagtg cgcctcctac gacccgctct
     1381 acaaggtcaa cccgccgctt cggacggcgg acgacgtgga ggcggtgaag gccggcctgg
     1441 ccgacggcgc catcggcatc atcgccaccg accacgcgcc ccacacggcc gagaccaagg
     1501 agctgccgtt cgaccaggca cctcccggca tgctcggcct cgagacggcg ctggccctcg
     1561 ccctcaccga gctcgacctg ccgatccagc aggtgctggc cgccctctcg tggaacccgg
     1621 ccgccgtcat cggcgccggc gacacccacg gactgcccgt ggccgagggc aacccggcca
     1681 acctctgcgt catcgacccc gaggccacct ggaccgtcga cggcgccgcc atggccagcc
     1741 gcagccacaa caccccctac caaggccgcg agctcacggg acgcgtccgc cacaccatcc
     1801 tggccggcga gcccgtcgtc atcggcggcg aagcccagcg ctgatgtccg atttctagaa
     1861 attcgttgcg agttctagaa gtcaggtcgt acgatgtctt tcatggcatt gagcatcaag
     1921 agcgacgagg cggaccgcct ggctcgtgag ctggtccagc tgaccggtga gaccctcacc
     1981 gatgcggtga ccgaggcgct gcgcgagcgg gtgcaacgca agcggccgga gcccaaggac
     2041 ccgcacgccg tgctggcccg cctccaggcc gagttcgccg cgaaccggac ggacgacaag
     2101 cggaccgacg acgagatcct gggctacgac gagttcggct tgccgtcgtg atcgtcgatt
     2161 cgtcggccgt cgtggcgatg ctcgcccgag aacccgatgc cgccgcgctc gaagcggcct
     2221 tggcacgtgc tgacgagccg gtcatcggtg cggcaacgtt cgtcgaggcg tcgacggtcg
     2281 tcgcggctcg catgcacgac ggagcgcgag cggtccgtga actgctcgag tcgttcggcg
     2341 tcgaggtcgt ggcgatcgat gaggccggcg cggctgctgc cgtcgacggt tggcggcggt
     2401 tcggtcgggg gaaccaccgt gcacgcctga acttcgggga ttgcctcacc tatggcgtgg
     2461 cggcggttcg cggcgagccg atcctgtgca tcgggaacga cttcgtgcag accgacgcga
     2521 ccgtcgtccc gctcggctga ccgctacttg aggccgggat ggcggctggc cagaaggggg
     2581 acgaggaggc gcttgggggc gaggtgggcg aaggtggcgc cgatgcggtt ggcggtgccg
     2641 gggatggcaa ccgggtggtt ggcggcgagg gcgtcgaggc cggccttggc gacggcctcg
     2701 ggcggctccc acatgaagct gggcaggctg gcctcggcct cgtccttgtc gaagccggcg
     2761 gcctcgccga agccggtgtc gaccgggccg gggcacaggg cggtggcgtg gacgccggtg
     2821 cccttgagct cgccgctgag gctgcgggtg taggagagca cgaaggcctt gcagccgccg
     2881 tagccggcct ggccggggag gggctggaag gcggccgtcg aggcgacgtt gaggatgccg
     2941 ccgcggcccc gttcgaccat gccgggcagg aagcggacgg tgaggtcgac gacggccacg
     3001 acgtcgagct cgatcatcgc catctcggcg tcgggatcgg ctgccgacac gcggcccagc
     3061 gtcgacaggc cggcgttgtt cacgaagatg tccggcacca gcccgaggcc ggtgacccgg
     3121 tcgggcagcc cggcgcgggc ggcgcggtcg gtcaggtcgg tggccagcac ctccgcgcgc
     3181 acgcctgagc cgcgcagctc ggcagccagg tcggccagct tgtcggcgga ccgggcgacg
     3241 agcgtgacgc cgtggcctcg gctcgccaga tcgcgggcga tggcggcgcc gatgcccgag
     3301 gacgctccgg tcacgacggc ggttcggtct ggagcgggcg tggggagggc catgcggcaa
     3361 cgctaggacc tggtggaacg cccggctcga ttcggagtcg gggggagcga ggcgcgggtg
     3421 gtaggttgtc ctcctgaccg tgaagtgggt cctgtgaggc ccgcacggac aggagagcgc
     3481 agatgtccca actcgtttcg ctgcacgagc cccctcgcgg gggctttctt tttgcccggg
     3541 cacgggccgt cggaggtctg cggtgaccgt tcgcgacgcc ctgctcgtgc tcaccgacgg
     3601 caccgtgttc gagggcgagg cgctcggcgc cacacccgcc tcgggcatcg ccaccggtga
     3661 ggtcgtcttc aacaccgtgc tctccggcta ccaggaggtg ctttcggatc cgtcctacgc
     3721 cgggcagatc atcaccttca cctacccgca catcggcaac tacggggtca ccgctgcgga
     3781 cgacgagtcc cgcacgccgt tctgccgggg cgtggtcgtg cgggagctgg cccgccgccg
     3841 ctccaactgg cgggccaccg aggacctcga cgagtggctc gcccgccacg gcgtccccgg
     3901 catcggcggc gtcgacaccc ggcgcctcac ccgccacatc cgcgacaccg gcgccatgcc
     3961 cggcg
//
LOCUS       DATMFS010000418         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_246015, whole genome shotgun sequence.
ACCESSION   DATMFS010000418 DATMFS010000000
VERSION     DATMFS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246015"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..994)
                     /locus_tag="VNQ33_07890"
     CDS             complement(242..994)
                     /locus_tag="VNQ33_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98065.1"
                     /translation="MHGGMRAAAVSLLLLTAGCASEAATKQATTAATGATTTTTAAAG
                     HHHGGDGADPDRPTIVDFHGSERQQLADQLVEVRELAMRYPTVADAEAAGYELTTPYA
                     PGTGAHYGKDADTQPPGQPLDLAKPQSFLYDGTEPDSRLVGVMYVQLGGDTAPDGFAG
                     PLDTWEPFPGQCLKQGTTDPVFPTKDSVTKEECDDAGGTFVDVTAWIQHVWVVPGWEA
                     PGGVFAPYNADIVCADGTTDADDVAGCESPAS"
     gene            complement(1020..1442)
                     /locus_tag="VNQ33_07895"
     CDS             complement(1020..1442)
                     /locus_tag="VNQ33_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWJ98066.1"
                     /translation="MSDLDDVNEFNRGIIEQFRANDGKVGPPFEGAPLLILHSTGAKT
                     GKDRLAPIVYQPVGDAWAVFASKAGAPDNPDWYHNLVAEPAARIELGSDEVEVTARVL
                     QGDERAEIWEKQKQLMPGFADYEAKTDRVIPVVLLERS"
     gene            complement(1453..2178)
                     /locus_tag="VNQ33_07900"
     CDS             complement(1453..2178)
                     /locus_tag="VNQ33_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98067.1"
                     /translation="MSEIPLHIGLIQCGHVHPDLAPTLGDYPELFADLLEPQGIDLTT
                     FDVDHDQFPDDLGGFDGWVVSGSANSAYEDLPWIHRTEELLRTLVAGQAPVVAVCFGH
                     QLLAQALGGRVAKSPDGWGAGMHRYELIGDPLPWMAPPPDGGVRIIASHQDQVVELPD
                     GAVVIARTDHCPIAAYTLGTSVLAIQPHPEFTTEVSRGLVERRRDRIGPETSDAALAS
                     LSEPLDQQLVAGWMAGFLRHARQ"
     gene            2217..2681
                     /locus_tag="VNQ33_07905"
     CDS             2217..2681
                     /locus_tag="VNQ33_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HWJ98068.1"
                     /translation="MTLMDIPTALHRGEGELPFVTIDEGIELQLLQVDIPNGLWVIRN
                     RFQPGSRVQKHKHTGQVFAFTQSGSWKYEEYPEVNTAGSYLFEPAGSEHTLIVPDSNT
                     EVTDVWFAIYGANLNLDAEGKVDLVIDAKMVLDFYVALCEAAGFANPPVIGA"
     gene            complement(2712..>3249)
                     /locus_tag="VNQ33_07910"
     CDS             complement(2712..>3249)
                     /locus_tag="VNQ33_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98069.1"
                     /translation="GTHTLERYRSQMLIQWRSFDVDVELHHHAGDTGVLFSHGDQGGG
                     YSLFIDDGDELVFAHNGYGVMTVLSGGTVPDGTEHIRLSVEAPGNWLWSASLAVDGQD
                     RARAEGLVMLGAISPFEGINVGIDRRSPVSWDVHERHGTFPYSGLLDAVTYTPGERAP
                     DAGTNFIDLLREIGLAYE"
BASE COUNT          454 a         1090 c         1200 g          505 t
ORIGIN      
        1 agaactcgtc gcatggtgtt tcctccaggt gatgtcggcg ggtgtctggg ccgccaccgg
       61 caccgatgtg atgccatgtg ctgaaggtac gaccgggtct ccgaggggtc atgagtagcg
      121 gatactcatc ttctcccggc cgacggcctg ctgcgggacg cgtccgtcgt ggcgcagtcc
      181 gacgacgttg ccgtcacgat cgcgcccgtg caaggaggtg cccgtcgccc cgggcggtcg
      241 gtcaggaggc gggtgactcg cagcccgcca cgtcgtcggc gtcggtggtg ccgtcggcgc
      301 acacgatgtc ggcgttgtag ggggcgaaca ccccgcccgg cgcctcccag cccgggacga
      361 cccacacgtg ctggatccag gcggtgacgt cgacgaaggt gccgccggcg tcgtcgcact
      421 cctccttcgt cacgctgtcc ttggtgggga acaccgggtc ggtcgtgccc tgcttcaggc
      481 actggccggg gaacggctcc cacgtgtcga gcgggccggc gaagccgtcg ggagccgtgt
      541 cgccgccgag ctggacgtac atgacgccga cgagccgcga gtcgggctcg gtcccgtcgt
      601 agaggaacga ctgcggcttg gccaggtcga gcggctgacc cggcggctgc gtgtcggcgt
      661 ccttgccgta gtgggcaccg gtccccggcg cgtacggcgt cgtgagctcg tacccggcgg
      721 cctcggcgtc ggccacggtg gggtaccgca tcgccagctc gcggacctcg acgagctggt
      781 cggccagctg ctggcgctcg ctgccgtgga agtcgacgat ggtcggccgg tccggatcgg
      841 cgccgtcacc gccgtggtgg tggcccgcgg cggcggtcgt ggtcgtcgtg gccccggtgg
      901 ccgcggtcgt ggcctgcttg gtggccgcct cgctcgcgca ccctgctgtc aggagcagca
      961 gcgagaccgc tgccgcccgc atccccccgt gcatggagga aacgtagccg gatggagggt
     1021 caggatcgct cgaggagcac gaccgggatc acccggtcgg tcttggcctc gtagtcggcg
     1081 aagccgggca tgagctgctt ctgcttctcc cagatctcgg cccgctcgtc gccttggagc
     1141 acgcgagcgg tcacttcgac ctcgtcggag ccgagctcga tcctcgccgc cggctcggcg
     1201 acgaggttgt ggtaccagtc ggggttgtcg ggcgcaccgg ccttggaggc gaacaccgcc
     1261 caggcgtcgc ccaccggctg gtacacgatg ggggcgaggc ggtccttgcc ggtcttggcg
     1321 cccgtgctgt gcaggatcag cagcggggcg ccctcgaagg gcggacccac cttgccgtcg
     1381 ttggcgcgga actgctcgat gatcccgcgg ttgaactcgt tgacgtcgtc gaggtcgctc
     1441 atggcgggca gcctattggc gggcgtggcg aaggaaaccc gccatccacc cggcgaccag
     1501 ctgctggtcg agcggctcgc tcaggctggc cagcgcggcg tcggacgtct ccgggccgat
     1561 gcggtcgcgc cggcgctcga cgaggccccg ggagacctcg gtggtgaact ccgggtgggg
     1621 ctggatggcg agcaccgacg ttccgagcgt gtaggcggcg atggggcagt ggtcggtgcg
     1681 ggcgatgacg acggcgccgt cgggcagctc caccacctgg tcctggtggc tggcgatgat
     1741 ccgcacccca ccgtcgggag ggggcgccat ccacggcagc gggtccccga tgagctcgta
     1801 gcggtgcatc cctgcgcccc agccgtcggg cgacttggcg acgcggccgc cgagcgcctg
     1861 ggcgagcagc tggtgcccga agcacaccgc cacgacgggc gcctggccgg cgaccagcgt
     1921 gcggagcagc tcctcggtgc ggtggatcca cggcaggtct tcgtacgccg agttggccga
     1981 accggagacg acccacccgt cgaacccgcc caggtcgtcg gggaactggt cgtggtcgac
     2041 gtcgaacgtc gtcaggtcga tgccctgcgg ctcgagcagg tcggcgaaca gctcggggta
     2101 gtcgccgagc gtgggggcca ggtccgggtg gacgtggccg cactggatga ggccgatgtg
     2161 cagggggatc tcagacacgg caggcaccgt accggccgac gtggttggct ttgggcatga
     2221 cgctcatgga catccccacc gccctgcacc gtggagaggg cgagctgccg ttcgtcacca
     2281 tcgacgaggg catcgagctc cagctcctcc aggtggacat cccgaacggc ttgtgggtca
     2341 tccgcaaccg cttccaaccc ggcagcaggg tccagaagca caagcacaca ggccaggtct
     2401 tcgccttcac ccagagcggg tcgtggaagt acgaggagta ccccgaggtc aacacggcgg
     2461 gcagctacct cttcgagccg gccggctccg agcacacctt gatcgtgccg gacagcaaca
     2521 ccgaggtgac cgacgtctgg ttcgccatct acggcgccaa cctcaacctc gacgccgagg
     2581 gcaaggtcga tctggtgatc gacgccaaga tggtgctcga cttctacgtg gccctctgcg
     2641 aggccgccgg cttcgcgaac ccgcccgtca tcggcgcctg accgctcgct cgccggtcac
     2701 tcgctcgccg gtcactcgta ggccaggccg atctcgcgga ggaggtcgat gaagttggtg
     2761 ccggcgtcgg gggcccgctc gccgggcgtg taggtgacgg cgtcgagcag gcccgagtac
     2821 gggaacgtcc catggcgctc gtggacgtcc cacgacacgg gggagcggcg gtcgatgccg
     2881 acgttgatcc cctcgaacgg ggagatggcg ccgagcatca cgagcccttc ggcccgggcg
     2941 cggtcctggc cgtcgacggc cagggacgcg ctccagagcc agttgcctgg agcctcgacc
     3001 gacaggcgga tgtgctcggt gccgtcgggc acggtgccgc cgctcagcac ggtcatcacg
     3061 ccgtagccgt tgtgcgcgaa caccagctcg tcgccgtcgt cgatgaacag cgagtagccg
     3121 ccgccttgat cgccgtggga gaacaagacg ccggtgtcgc cggcgtggtg gtggagctcg
     3181 acgtcgacgt cgaacgaccg ccactggatc agcatctggg agcggtagcg ctcgagcgtg
     3241 tgggtgccg
//
LOCUS       DATMFS010000419         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_175510, whole genome shotgun sequence.
ACCESSION   DATMFS010000419 DATMFS010000000
VERSION     DATMFS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175510"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VNQ33_07915"
     CDS             <1..330
                     /locus_tag="VNQ33_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98070.1"
                     /translation="TEWMVTQGGATKARSTTVQVANPGDSSAQITVITLSSRDREVLR
                     SDISIPPGDRRSLDLDGAGSGATVIVQSSRPVVVGSSISLAEGPGIAVQLASPYPETV
                     VALPPVS"
     gene            344..808
                     /locus_tag="VNQ33_07920"
     CDS             344..808
                     /locus_tag="VNQ33_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWJ98071.1"
                     /translation="MMQLATVAIVGAIAGVVAWSLQRRRSPEPERGPSWTVPQLVSRA
                     DFDRPEAPWLVVLFSSSTCLACRGTAAKAQLLASDEVAYQEVDHVEHQDLHDRYGIDA
                     VPLLLLVDPRGSVRRSFVGEPTATDLWAALAELREPGSVPPGCGRSDGCDHD"
     gene            complement(828..>2065)
                     /locus_tag="VNQ33_07925"
     CDS             complement(828..>2065)
                     /locus_tag="VNQ33_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ98072.1"
                     /translation="VAGEAGVGFLSVTGSDFMEMFVGVGASRVRDLFQSARKLGRAII
                     FVDEIDSIGRKRGAGLGGGHDEREQTLNQMLSEMDGFEATEGIVMIAATNRPDVLDPA
                     LLRPGRFDRQVVVPLPEHSERLAILRVHAKSKRISPDVDLSIVARGTPGMSGAELANL
                     VNEAALYAVRRGAEMITREDFEYARDRVLMGQRRESMALSDPEKELTAYHEAGHAVCA
                     TILEDADPVHKVTILPMGMALGVTQQLPSEDRHSYNQEYLEQSIVVAMGGRIAEKLVF
                     GVVSTGANNDLVVSTERARKMVREWGMSERIGPMAWGSQGQVFLGEDLMGNARDYSDE
                     TARVIDQEVEQILRDCERRCEELLTTNRNGLDLVARSLLEHETIDGAEVRRLCDLGAN
                     PSTNLHHEPEPEPASRTDQ"
BASE COUNT          269 a          728 c          751 g          317 t
ORIGIN      
        1 accgagtgga tggtcacgca gggcggtgcc accaaggccc gctccaccac cgtgcaggtc
       61 gccaaccccg gtgactcgtc ggcgcagatc acggtcatca ccctgtccag ccgggaccgt
      121 gaggtcctcc gatcggacat ctcgatcccg cccggcgacc gccggtccct ggacctcgac
      181 ggtgccggct cgggcgccac ggtgatcgtg cagagctccc ggccggtggt ggtgggatcg
      241 tcgatctcgc tggccgaggg cccgggcatc gcggtccagc tggcctcgcc gtacccggag
      301 accgtggtgg cgctcccgcc ggtcagctag ccagactggg gccatgatgc agctggccac
      361 cgtggcgatc gtgggtgcga tcgccggggt cgtcgcctgg tccctgcagc gtcgccgttc
      421 acccgagccc gagcggggcc cgtcgtggac cgtgccgcag ctcgtcagcc gggccgactt
      481 cgaccggccc gaggccccgt ggctggtcgt gctgttctcg tcgtcgacgt gcctggcctg
      541 ccgaggcacc gccgccaagg cccagctcct cgcctcggac gaggtcgcct accaggaggt
      601 cgaccacgtc gagcaccagg acctccacga ccgctacggc atcgacgccg tcccgctgct
      661 cctgctcgtc gaccccaggg ggtcggtccg ccgcagcttc gtcggcgagc cgacggcgac
      721 cgacctctgg gctgcgctcg ccgagctgag ggagccgggg tcggtcccgc ccggctgcgg
      781 ccgctccgac ggctgcgacc acgactgacg accgaacccg cggcgagcta ctggtcggtg
      841 cgggacgccg gctcgggctc gggctcgtgg tgcaggttgg tggaggggtt ggcgccgaga
      901 tcgcacaggc gccgcacctc ggcgccgtcg atggtctcgt gctcgaggag gctgcgggcc
      961 accaggtcca ggccgttgcg gttggtggtg aggagctcct cgcagcgccg ctcgcagtcg
     1021 cggaggatct gctcgacctc ctggtcgatg acgcgggccg tctcgtccga gtagtcgcgg
     1081 gcgttgccca tgaggtcctc gcccaggaac acctggccct gcgagcccca ggccatgggc
     1141 ccgatgcgct cgctcatgcc ccactcgcgg accatcttgc gagcccgttc ggtggacacg
     1201 acgaggtcgt tgttggcgcc ggtggagacc acgccgaaca cgagcttctc ggcgatgcgc
     1261 ccgcccatgg cgaccacgat cgactgctcc aggtactcct ggttgtagga gtggcggtcc
     1321 tcggacggca gctgctgggt gacacccagg gccatgccca tggggaggat ggtcaccttg
     1381 tggacgggat cggcgtcttc gaggatcgtg gcgcacacgg cgtggccggc ctcgtggtag
     1441 gcggtgagct ccttttcggg atccgacagc gccatcgact cccggcgctg gcccatgagc
     1501 acccggtcac gggcgtactc gaagtcctcc cgggtgatca tctcggcgcc gcggcgcacg
     1561 gcgtagagcg cggcctcgtt gaccaggttg gccagctcgg cgccgctcat gcccggggtg
     1621 ccgcgggcca cgatgctcag gtcgacgtcg gggctgatgc gcttggactt ggcgtgcacc
     1681 cgcaagatgg cgaggcgctc ggagtgctcg ggcagcggga ccacgacctg gcggtcgaag
     1741 cggcccggcc gcagcagcgc cggatcgagg acgtcgggcc ggttggtggc ggcgatcatc
     1801 acgatgccct cggtggcctc gaagccgtcc atctccgaca gcatctggtt gagggtctgc
     1861 tcgcgctcgt cgtgcccgcc gccgaggccg gcgccgcgct tgcggccgat ggagtcgatc
     1921 tcgtccacga agatgatggc ccggcccagc ttgcgggcgg actggaacag gtcgcggacg
     1981 cggctggcgc cgacacccac gaacatctcc atgaagtccg agccggtgac cgagaggaag
     2041 ccgacgccgg cctcgccggc gacgg
//
LOCUS       DATMFS010000420         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_154301, whole genome shotgun sequence.
ACCESSION   DATMFS010000420 DATMFS010000000
VERSION     DATMFS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154301"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /gene="nirB"
                     /locus_tag="VNQ33_07930"
     CDS             <1..1669
                     /gene="nirB"
                     /locus_tag="VNQ33_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HWJ98073.1"
                     /translation="AIGECAAVEGTCYGLVAPGYSTAEVVADRLVGGTAEFPGADLST
                     KLKLLGVEVANVGSVHDLEGCTSLVWDDPVGATYQRLDVDADGRVRAAVLVGDASAFG
                     LVLDRFRSGDAVAAPGELLKATAPPDPTAAPDPSAGVCSCENVSRGAVCGAIAEGCDT
                     LAALKAATGAGTGCGGCIPLLEGLLRTESTGTPPGVCEHFALTRAELYDLIRVRRHTT
                     FAEVLDRYGAAAGGCEVCKPVVASILASLDNGYILDGEQAALQDSNDHFLANIQRDGT
                     YSVVPRVPGGEITPAGLIAIGEVARDFDLYTKITGGQRIDLLGARVEQLPAIWRRLVD
                     AGFESGHAYGKSLRTVKSCVGSSWCRYGVQDSTSMAILLEERYRGLRSPHKIKMAVSG
                     CARECAEAQSKDVGVIATERGWNLWVGGNGGMRPRHAELLAEDLDDAALIRSIDRFLE
                     YYVRCADRLQRTASWIEGLDGGVDHVRQVVVEDSLGLGAELEAAMAAHVAAYECEWAA
                     TLERPDRLARFVTYVNAPDEPDPTVVFVRERGQIRPARSDELVGANR"
     gene            1666..2058
                     /gene="nirD"
                     /locus_tag="VNQ33_07935"
     CDS             1666..2058
                     /gene="nirD"
                     /locus_tag="VNQ33_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWJ98074.1"
                     /translation="MTLLDAERTTWTAVCPVAHLTPGRGAAVLVDGEALAVFLLADGS
                     VRAIGNIDPCSGASVLSRGLVGHTEREGVTLRYVASPLRKQRFDLDTGCGLDADADAG
                     SWPVRVVDGFVEVGRQASPWGNGPETAP"
BASE COUNT          293 a          818 c          781 g          299 t
ORIGIN      
        1 cgccatcggc gaatgcgccg ccgtcgaggg cacctgctac ggactcgtcg cccccggcta
       61 cagcaccgcc gaggtcgtcg ccgaccggct cgtcggcggc accgccgagt tccccggcgc
      121 cgacctgtcg acgaagctca agctgctcgg cgtcgaggtc gccaacgtcg gatcggtcca
      181 cgacctcgag gggtgcacca gcctggtgtg ggacgacccg gtcggtgcca cctaccagcg
      241 cctggacgtc gatgccgacg gccgggtgcg agccgcggtc ctcgtgggcg acgcgtcggc
      301 gttcgggctg gtcctcgacc ggttccggag cggcgatgcc gttgccgcgc caggggagct
      361 gctcaaggcg acggcaccgc cggacccgac cgctgcgccc gacccgtcgg cgggcgtctg
      421 ctcgtgcgag aacgtgagcc gcggcgccgt gtgcggcgcg atcgccgagg gctgcgacac
      481 cctcgccgcc ctcaaggccg ccaccggtgc cggcacgggg tgcggcggct gcatcccgct
      541 gctggaaggg ctgctccgca cggagtcgac cggcaccccg ccgggggtgt gcgagcactt
      601 cgccctcacc cgcgccgagc tctacgacct gatccgggtc cgccgccaca cgaccttcgc
      661 cgaggtcctc gaccggtacg gcgcagccgc cgggggctgc gaggtctgca agccggtggt
      721 cgcctcgatc ctcgcctcgc tcgacaacgg ctacatcctc gatggcgagc aagcggcgct
      781 ccaggacagc aacgaccact tcctcgccaa catccagcga gacgggacct actcggtcgt
      841 cccccgggtc cctggcggcg agatcactcc ggcgggcctc atcgcgatcg gtgaggtggc
      901 tcgggacttc gacctctaca cgaagatcac gggcggccag cgcatcgacc tgctcggcgc
      961 gcgggtcgag cagctgccgg cgatctggcg gcggctggtc gacgccggct tcgagtcggg
     1021 ccacgcctac ggcaagtccc tccgcaccgt gaagtcctgc gtgggcagca gctggtgccg
     1081 ctacggggtc caggactcga cgtcgatggc catcctcctc gaagagcgct accggggcct
     1141 gcgctcgcct cacaagatca agatggccgt gtcgggctgc gcccgcgagt gcgccgaggc
     1201 gcagagcaaa gacgtcggcg tgatcgccac cgagcggggg tggaacctct gggtgggcgg
     1261 caacggcggc atgcgccccc gccacgccga gctgctggcc gaagacctcg acgacgctgc
     1321 cctgatccgc tcgatcgacc ggttcctcga gtactacgtg cgctgcgccg accgcctcca
     1381 gcgcacggcg tcgtggatcg aggggctcga cggcggcgtc gaccacgtgc gccaggtcgt
     1441 cgtggaggac tcgctcgggc tgggcgccga gctcgaggcg gccatggcgg cgcacgtggc
     1501 cgcctacgag tgcgagtggg ccgccacgct cgagcgcccc gaccggctgg cgaggttcgt
     1561 gacctacgtg aacgcgcccg acgagcccga tcccaccgtc gtgttcgtgc gtgagcgcgg
     1621 ccagatcaga cctgcccgat ccgacgagct cgtgggagcg aaccgatgac cctgctcgat
     1681 gccgagcgca ccacctggac cgcggtgtgc cccgtggccc acctgacccc cggccggggc
     1741 gcggccgtcc tcgtcgacgg cgaggccctc gccgtcttcc tgctcgccga cggttcggtg
     1801 cgggcgatcg gcaacatcga cccgtgctcg ggcgcctcgg tcctgtcgcg gggcctggtg
     1861 ggccacacgg aacgtgaggg cgtcacgctc cgctacgtcg cctcaccgct ccgcaagcag
     1921 cgcttcgacc tcgacaccgg gtgcggcctc gacgccgacg ccgacgccgg cagctggccg
     1981 gtccgggtgg tcgatggctt cgtcgaggtg ggccggcagg catcaccttg gggcaacggg
     2041 cccgaaaccg ccccgtaacc gccaccgtgg acactgcccc cgtgaccctg ggatccctca
     2101 gcggcttcac ggtcgccgtg accgcggacc gccgcagcga ggagcaggcc tcgctgatcg
     2161 cccgccgtgg gggcgaggtc gtgcgcggac c
//
LOCUS       DATMFS010000421         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_131079, whole genome shotgun sequence.
ACCESSION   DATMFS010000421 DATMFS010000000
VERSION     DATMFS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131079"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VNQ33_07940"
     CDS             <1..280
                     /locus_tag="VNQ33_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98075.1"
                     /translation="EGDAVQFHSCDPGKDAKAVGTEVTPDTLGLPVTRTDVYTSSIEQ
                     QATPKQSACYANGVIDAFTVAQLEDPKGTFILSDAGQLILESVRKKCV"
     gene            complement(320..805)
                     /locus_tag="VNQ33_07945"
     CDS             complement(320..805)
                     /locus_tag="VNQ33_07945"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HWJ98076.1"
                     /translation="MAFFRRTPAVRRRPDGAFDLTLDAKRREVLVSLLRQLADLLETQ
                     PDHPNLTRLHPPAYLDDPDADAGYQLLAGEELRDSQRASIDAVVESLGRDQLTEDEMW
                     SWVQSLNGVRLVVGTRLEIAEDDHSPSLRSIPEPEDRALWVVYDFATELQHDVIEALS
                     R"
     gene            complement(812..1117)
                     /gene="clpS"
                     /locus_tag="VNQ33_07950"
     CDS             complement(812..1117)
                     /gene="clpS"
                     /locus_tag="VNQ33_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWJ98077.1"
                     /translation="MASSTPIISPTEISSPEDDLGLDTDQPWIVLVWNDPINTMEYVT
                     LVFQKLFGYSKEKAEKLMLDVHNLGRAVVSSGAREKAEIDVFRLHEHGLWATMQHDE"
     gene            complement(1068..2084)
                     /locus_tag="VNQ33_07955"
     CDS             complement(1068..2084)
                     /locus_tag="VNQ33_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWJ98078.1"
                     /translation="MLPRLRQPVSPAAYRTITMVALVLLVAIVVTGAGVRLTGSGLGC
                     TDWPRCTEETFTPSSNLHAQIEFYNRMITGLVSVAVVLAVLGSLRRSPRRQDLVWLSW
                     GLVGGVVGQIVLGGIVVLTHLNPWLVQGHFVVSMILVADAAVLTYRAGLPDGVAQRPI
                     VTPALLRWGKALVGLGIVVLLTGTLVTGSGPHSGHNEAELGASASEKVEAAREVRRLP
                     IHVHDAARIHGITMILFLAATIWVLVQIRHHQPAGKLAATAGWLLTALILQGAIGYTQ
                     YFTGVPPGLVALHILGASLVWVAVLFTLQRMWAPLVPAGPGAAQSTGGIEHPDHLPDG
                     DLVP"
     gene            2200..>2660
                     /locus_tag="VNQ33_07960"
     CDS             2200..>2660
                     /locus_tag="VNQ33_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWJ98079.1"
                     /translation="MLKPAPSPLRARLAGYVALTKPRIIELLLITTVPTMVVANQGIP
                     SGWLILNTIIGGAFAAGGANAINMYVDRDIDAVMERTKNRPLVTGLIKPRNGLIFAIG
                     LEVVAFAWLTSWVNLLTAALGVAACLFYVFVYTLWLKRTSKSNIVIGGAAGA"
BASE COUNT          426 a          897 c          962 g          375 t
ORIGIN      
        1 cgagggcgat gcggtccagt tccacagctg cgatccgggc aaggacgcca aggcggtggg
       61 caccgaggtc acgcccgaca cgctcggcct ccccgtcacc cggaccgacg tctacacgag
      121 ctccatcgag cagcaggcga cgccgaagca gtcggcttgc tacgcgaacg gcgtcatcga
      181 cgccttcacg gtggcccagc tggaagaccc gaagggcacg ttcatcctct ccgacgccgg
      241 acagctgatc ctcgagagcg tccgcaagaa gtgcgtctga tctgagggcg cccgccggcc
      301 cgcccgcgcc gcggtcgggt cagcggctca gcgcctcgat gacatcgtgc tggagctcgg
      361 tcgcgaagtc gtagacgacc cagagcgccc ggtcctcggg ttcggggatc gagcgcagtg
      421 acgggctgtg gtcgtcctcg gcgatctcca gccgtgttcc caccacgagg cggacgccgt
      481 tgagcgactg cacccacgac cacatctcgt cctcggtgag ctggtcccgc ccgagcgact
      541 cgaccacggc gtcgatggag gcccgctggg agtcgcgcag ctcctcgccg gcgaggagct
      601 ggtagccggc gtcggcgtcg gggtcgtcga ggtaggcggg cggatggagc cgggtcaggt
      661 tcggatggtc cggctgggtc tcgagcaggt cggcgagctg gcggagcagc gacacgagga
      721 cctcgcggcg cttggcgtcg agggtcaggt cgaaggcgcc gtcggggcgg cgcctgacgg
      781 ccggggtcct gcggaagaag gccatggccg gctactcgtc gtgctgcatg gtggcccaca
      841 gcccgtgctc gtggaggcgg aacacgtcga tctcagcctt ctcccgtgca cccgacgaga
      901 cgacggcgcg gccgaggttg tgcacgtcga gcatcagctt ctcggccttc tccttcgagt
      961 acccgaacag cttctggaac acgagggtga cgtactccat ggtgttgatg ggatcgttcc
     1021 agacgagcac gatccagggc tggtccgtgt cgaggcccag atcgtcctca ggggacgaga
     1081 tctccgtcgg ggagatgatc ggggtgctcg atgccacccg tcgattgtgc cgcgcccgga
     1141 ccggccggca ccagaggcgc ccacatccgc tgcagcgtga agagcacggc gacccacacg
     1201 agagacgcgc cgaggatgtg caaggcgacg agtccaggtg ggacgccggt gaagtactgc
     1261 gtgtagccga tggcgccctg gaggatcagc gcggtgagga gccacccggc cgtcgccgcc
     1321 agcttccctg ccggctggtg gtggcggatc tgcaccagca cccagatggt ggcggcgagg
     1381 aacaggatca tcgtgatgcc gtggatgcgg gccgcgtcgt gcacgtggat gggcagccgg
     1441 cgcacctccc gcgccgcctc gaccttctcg ctggcggagg cgccgagctc ggcctcgttg
     1501 tgcccgctgt ggggacccga ccccgtgacc agggtgccgg tgaggagcac gacgatgccg
     1561 agcccgacga gcgccttgcc ccaccggagc agagccgggg tcacgatcgg ccgttgcgcc
     1621 accccgtcgg gcagcccggc ccggtaggtg agcacggcgg cgtcggccac gaggatcatc
     1681 gacaccacga agtgcccctg caccagccac gggttcaggt gggtgagcac gacgatgccg
     1741 ccgagcacga tctggccgac gacaccgccc accagccccc acgacagcca gaccaggtcc
     1801 tgacggcgag gtgaccggcg gagcgacccg agcacggcca ggaccacggc gacggacacc
     1861 aggcccgtga tcatgcggtt gtagaactcg atctgggcgt ggaggttcga cgagggggtg
     1921 aacgtctcct ccgtgcagcg cggccagtcg gtgcagccga ggcccgaccc ggtcaggcgc
     1981 acgccggccc cggtgacgac gatggccacc aggagcacga gggcgaccat cgtgatcgtc
     2041 cggtaggccg ccggggagac cggttggcgg aggcggggga gcacgcggcc agggtacggg
     2101 cgggcccggt ggtgcggcca agccgcaggt gacggcgccg agacgggcgc cagcctgcgg
     2161 actcgtctgg ggtgcgcgcc gagacgtagg gtcgagaacg tgttgaagcc ggccccgtca
     2221 ccactgcgag cccgactcgc cgggtacgtg gcgttgacca agccgcgcat catcgagctg
     2281 ctgctcatca ccacggtgcc caccatggtg gtcgcgaacc agggcatccc gtcgggctgg
     2341 ctgatcctga acacgatcat cggcggcgcc ttcgcggctg gcggcgccaa cgccatcaac
     2401 atgtacgtcg accgcgacat cgacgccgtg atggagcgga ccaagaaccg gccgctggtc
     2461 acgggcctca tcaagccccg caacggcctg atcttcgcca tcggcctcga ggtcgtggca
     2521 ttcgcctggc tcacctcctg ggtcaacctg ctcacggcgg ccctcggcgt ggccgcgtgc
     2581 ctcttctacg tgttcgtgta cacgctgtgg ctcaagcgga cgtccaagag caacatcgtc
     2641 atcggcggtg cggcgggcgc
//
LOCUS       DATMFS010000422         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_60680, whole genome shotgun sequence.
ACCESSION   DATMFS010000422 DATMFS010000000
VERSION     DATMFS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60680"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VNQ33_07965"
     CDS             <1..763
                     /locus_tag="VNQ33_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98080.1"
                     /translation="WRHLLPELAAAGYRAVAPFLRGYAPTQVPDDGLYQPGAVASDAI
                     ALHDALGGDGDAVLIGHDWGALATYAAAGHAPERWAKAVVASVPPPATVAQAFFSYDQ
                     LQRSWYMFFFQSPLSDLAVPMDDLAFIDRLWRDWSPGYDGAADAGRAKDCFRDPANLA
                     AALGYYRATLGAGARSPEYDAVEAAGAAPLSVPALYLHGADDGCMGAELIDDGVLASL
                     AGPGSRYEVVPDAGHFLQLEQPDAVNRLILDFLQA"
     gene            complement(1263..2147)
                     /locus_tag="VNQ33_07970"
     CDS             complement(1263..2147)
                     /locus_tag="VNQ33_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWJ98081.1"
                     /translation="MTRNRREARHGALLDWFGHRQRDLPWRRTRDPWAVLVSELMLQQ
                     TQVARVLPKYEAFLAQWPTPAACAAAPLGDVVTAWAGLGYNRRAVNLHRCAQQVVAGH
                     GGSLPEDLDALLALPGIGPYTARAVLAFAFESDEVGVLDTNAARVLARWSGRSLRPKE
                     ARALADASVAPAAGWAWNQAMLDLGATVCVPRTPRCGECPVAEWCTWHREGLPELDPA
                     VGSAGVSTGQSRFDGSDRQGRGRLVDALRVGPVPAADLAAVMGWPDDPARATRVAATV
                     VADHLATVDHDGTHHLPS"
     gene            complement(2275..>2485)
                     /locus_tag="VNQ33_07975"
     CDS             complement(2275..>2485)
                     /locus_tag="VNQ33_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98082.1"
                     /translation="RPAERYDSSYIQPMDPDTLVQRVLSTSYIARLPAAEQSALAEKV
                     RELVAPLGASFELPYLSVAYAAPLR"
BASE COUNT          325 a          982 c          889 g          289 t
ORIGIN      
        1 ctggcggcac ctcctgcccg agctggcggc ggccggctac cgggccgtcg ccccgttcct
       61 gcgcggctac gcccccaccc aggtccccga cgacgggctg taccagcccg gcgccgtcgc
      121 gtcggatgcc atcgcgctcc acgacgcgct gggcggcgac ggcgacgccg tgctcatcgg
      181 ccacgactgg ggcgccctcg ccacctacgc cgccgccggg cacgcgcccg agcggtgggc
      241 caaggccgtc gtcgcgtcgg tgcccccacc cgccaccgtc gcccaggcgt tcttctccta
      301 cgaccagctg cagcggtcct ggtacatgtt cttcttccag tcgccgctgt cggacctggc
      361 cgtgccgatg gacgacctcg ccttcatcga ccgcctctgg cgggactggt cacccggcta
      421 cgacggcgcc gccgacgccg gccgagccaa ggactgcttc cgcgacccgg ccaacctggc
      481 cgccgcgctc gggtactacc gagccaccct cggcgccggt gcccgctcac ccgagtacga
      541 cgcggtcgag gccgccggcg ccgcaccgct gtcggtcccg gcgctctacc tccacggcgc
      601 ggacgacggc tgcatgggcg ccgagctcat cgacgacggc gtcctcgcct cgctggccgg
      661 ccccggctcc cgctacgagg tcgtgcccga cgccggccac ttcctgcagc tcgagcagcc
      721 cgacgccgtc aaccgcctca tcctcgactt cctccaggcc tgaccggcgc gccgacgcgg
      781 ccgtccacga gggccgggtg ccggcgaccg gtgagcgccg ctccgtggtc ggctcgtgcg
      841 acggcacccc tcgccgtcgg ggccgatcga ctcgtcgtct cgggcgagcc ccacgccaac
      901 ggcccgcgcc gcagccgacg gtgatcgagc gcgccgacgc ggccgcgctc atcctccgct
      961 gcggcctgtt cggccgtgcc ctccagcggg agacgagggc ggggttctgc tccccgccgt
     1021 gcgcaccaac gacgcccgcg ccctgctcga cggcctccga gcccacggct ggcccgcccg
     1081 gacgaccggc tggcgtcggc cccgccgacc gcgacgaggc acgcttctcg tcgcacggac
     1141 ggctcagagc gagccacctc agcgaccaga acaggaagcg acccgccccc cgacgacgtg
     1201 cgtgccggct cggcccgagg tcgagccgca ggcttcaggc ttcaggcgtc aggcgtcagg
     1261 cgtcagctgg gcaggtggtg ggtgccgtcg tggtcgacgg tggcgaggtg gtcggcgacc
     1321 accgtggccg cgacccgggt cgcccgagcg ggatcgtcgg gccagcccat gaccgcagca
     1381 aggtcagccg ccggcacggg accgacccgg agggcgtcga ccaagcgccc tcggccctgg
     1441 cgatcgctcc cgtcgaagcg ggactggccg gtcgacacgc ccgctgagcc gacggcggga
     1501 tccagctccg gcaacccctc ccggtgccac gtgcaccact cggcgacggg gcactcgccg
     1561 caccgcgggg tgcggggcac gcagacggtg gcgccgaggt cgagcatcgc ctggttccac
     1621 gcccagcccg ccgccggcgc gaccgaggca tcggccaggg cccgggcctc cttcggcctc
     1681 agcgaccgac cggaccagcg ggccaggacc cgggcggcgt tggtgtcgag cacgcccacc
     1741 tcgtcggact cgaaggcgaa ggccagcacg gcccgggcgg tgtagggccc gatcccgggg
     1801 agggcgagca gcgcatcgag gtcctcgggc aatgagcccc cgtgcccggc gaccacctgc
     1861 tgggcgcagc ggtggaggtt cacggcgcgc cggttgtacc cgaggccagc ccaggcggtg
     1921 accacgtcgc ccaggggcgc ggcggcgcac gcggccggcg tcggccactg ggctaggaac
     1981 gcctcgtact tcggcaggac tcgggccacc tgggtctgct gcagcatcag ctcggacacg
     2041 agcaccgccc acgggtcccg cgtccgccgc cacggcagat cccgctgccg gtgcccgaac
     2101 cagtcgagca gcgccccgtg ccgcgcctcc cgccgatttc gggtcacgcg agccccacca
     2161 cggtgcacag acgacccgaa acaccacgga gccggcgacc acctcgattt cgggtcacgc
     2221 gagccccgcc acggtgcaca ggtgacccga aatcgtggga ggcatcgggg gcggtcagcg
     2281 gaggggggcg gcgtaggcca ccgacaggta cgggagctcg aacgaggcgc cgagcggggc
     2341 gacgagctcg cggaccttct ccgccagggc ggactgctcg gcggcgggaa ggcgcgcgat
     2401 gtacgacgtc gacagcaccc gctgcacgag ggtgtcgggg tccatgggct ggatgtacga
     2461 cgagtcgtag cgctcggcgg gccgg
//
LOCUS       DATMFS010000423         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_154383, whole genome shotgun sequence.
ACCESSION   DATMFS010000423 DATMFS010000000
VERSION     DATMFS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154383"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="coaBC"
                     /locus_tag="VNQ33_07980"
     CDS             complement(<1..1077)
                     /gene="coaBC"
                     /locus_tag="VNQ33_07980"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWJ98083.1"
                     /translation="MFAGRRIVLGVTGGIAAYKAVEVCRRLVDAGAHVAPIMTKGASR
                     FIGETTLSALASEPVQTSLWDEDSPIPHTRLGQGADLVLVAPATARLLADYAAGRSAD
                     LLTATLLATRAPVLVCPAMHTEMWEHPAVQDNLATLRRRGVRIVEPESGRLAGGDLGH
                     GRLADPASIVAAAGAALSIPDLAGLRVLVTAGGTREPIDPVRFIGNRSSGKQGTAIAL
                     AALRRGADVTLITAAAAPALPGLDVVTVATAAELRAAVHERAPQADVVVMAAAVADFR
                     PVTAAADKIKKAGGIPEIRLEPTEDILAELGAAKAPGQTLVGFAAETTDVAANAQDKL
                     ARKRADLLVANDVGAPATGFEHDTN"
     gene            complement(1105..1416)
                     /gene="rpoZ"
                     /locus_tag="VNQ33_07985"
     CDS             complement(1105..1416)
                     /gene="rpoZ"
                     /locus_tag="VNQ33_07985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWJ98084.1"
                     /translation="MTWQHETMMNPPIEELLTRADSKFRLVTLGAKRARQINSYFGQL
                     GDTSGSLVPPQVTSVARKPLSIAFEEIAADKIEAVQIDPEADAAEAQAALDAAALDAG
                     A"
     gene            complement(1548..>2009)
                     /locus_tag="VNQ33_07990"
     CDS             complement(1548..>2009)
                     /locus_tag="VNQ33_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HWJ98085.1"
                     /translation="GLDHLDGGTVFRTMASERGVTLAEFARIAEADDAIDRALDDRLV
                     ERARDGDVLLESRLAGWLATRAALRALRIWIHCDEVERARRVGGRDGHGVDDALAVNQ
                     EREASERARYQDFYGIDLQDLSIYDLVIDSTTTDPAAITDQIISVAGGTRT"
BASE COUNT          259 a          674 c          789 g          287 t
ORIGIN      
        1 gttggtgtcg tgctcgaaac cggtggccgg cgcgccgacg tcgttggcga ccaggagatc
       61 ggcccgcttg cgagccaact tgtcctgggc gttggcggcg acgtcggtgg tctcggccgc
      121 gaaccccacg agcgtctggc cgggagcctt ggctgcaccg agctcggcga ggatgtcctc
      181 ggtgggctcg aggcggatct ccgggatgcc gccggccttc ttgatcttgt cggcggccgc
      241 ggtgacgggc cggaagtcgg ccacggcggc ggccatcacg accacgtcgg cctgcggtgc
      301 ccgttcgtgc acggcggcgc gcagctcggc ggcggtggcg acggtgacga cgtcgagccc
      361 gggaagggcc ggtgctgctg ctgcggtgat gagcgtgacg tcggcgcctc gccggagcgc
      421 ggcgagggcg atcgcggtcc cctgcttgcc cgacgagcgg ttgccgatga accgcaccgg
      481 gtcgatgggc tcgcgcgtgc cgccggcggt gacgagcacc cggaggccgg cgaggtcggg
      541 gatcgagagg gcggcaccgg cggcggccac gatcgaggcg gggtcggcca gacgcccgtg
      601 gccgaggtcg ccgcccgcga gccggccgct ctcgggttcg acgatgcgga cgccacggcg
      661 ccggagcgtg gcgaggttgt cctgcaccgc cgggtgctcc cacatctccg tgtgcatcgc
      721 cgggcacacc agcaccggcg cccgcgtggc gagcagcgtg gcggtgagca ggtcggccga
      781 gcggccggcg gcgtagtcgg ccagcaggcg cgcggtggcc ggcgccacga ggaccagatc
      841 ggccccctgg ccgagccggg tgtgcgggat cgggctgtcc tcgtcccaga gcgaggtctg
      901 gaccggctcg gaggccaggg ccgacagggt cgtctcgccg atgaagcgcg aggcgccctt
      961 cgtcatgatg ggcgccacgt gggcgcccgc gtcgacgagg cgccggcaga cctcgacggc
     1021 cttgtacgcg gcgatcccgc cggtcacgcc gaggacgatg cgccgaccgg cgaacatggc
     1081 aggggattca gccaggcagg tggatcaggc gccggcgtcg agcgccgcgg catcgagcgc
     1141 cgcctgggcc tcggcggcat cggcctcggg gtcgatctgg acggcctcga tcttgtcggc
     1201 ggcgatctcc tcgaaggcga tcgacagcgg cttgcgggcg accgacgtga cctgcggcgg
     1261 caccaggctg ccggacgtgt cgcccagctg gccgaagtac gagttgatct gacgggcgcg
     1321 cttggcgccc agcgtcacga gccggaactt cgagtccgcc cgggtgagga gctcctcgat
     1381 cggggggttc atcatcgtct cgtgctgcca ggtcatggtg tccgtgtcct tcgtcgggtg
     1441 cgccgtgagg ccggtcgatc gtaccaaccc gaggcgccgc cgagcgagac gggggcgtcg
     1501 cctcacccgc gcgggggcgt gaccggcagc cagcgcgtcc cggtgggtca ggtgcgagtg
     1561 ccgccagcga ccgagatgat ctggtcggtg atcgcggcgg ggtcggtggt cgtggagtcg
     1621 atgaccaggt cgtagatgga caggtcctgg aggtcgatgc cgtagaagtc ctggtagcga
     1681 gcacgttccg aggcttctcg ctcctggttg accgccagcg catcgtcgac cccgtggccg
     1741 tctcgtccgc cgacccggcg ggcccgctcg acctcgtcgc agtggatcca gatgcgcagg
     1801 gcacgaagcg cagctctggt ggcgagccat cccgcgaggc gggactccaa caagacgtcg
     1861 ccgtcacgag cccgctccac caagcggtcg tcgagggcgc ggtcgatcgc atcgtcggct
     1921 tcggcgatgc gtgcgaactc ggcgagcgtc acgccccgct cggacgccat ggtccgaaac
     1981 acggtcccgc catcgaggtg gtcgagccc
//
LOCUS       DATMFS010000424         7288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_131103, whole genome shotgun sequence.
ACCESSION   DATMFS010000424 DATMFS010000000
VERSION     DATMFS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131103"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..229)
                     /locus_tag="VNQ33_07995"
     tRNA            complement(155..229)
                     /locus_tag="VNQ33_07995"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Gln,seq:ctg)
     gene            complement(306..1145)
                     /locus_tag="VNQ33_08000"
     CDS             complement(306..1145)
                     /locus_tag="VNQ33_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ98086.1"
                     /translation="MSTPSSELPLTEQVQHRVDERGIASITLNRPDVKNAISPDQRDR
                     VIALLGEASADLGVRAVVLGHTGDAFCTGADLRASRPPAPRPDDAPKRGVGEVAKLIR
                     EGAQALVAAILDCEKPVIAAVNGTAAGIGAHIALACDLVVAADGVRFIEVFVRRGLVP
                     DGGGAYLLPRIVGVRRAKELLFFGDDLSATEAAELGLINKVVPAGELDDAVEAWAARL
                     AVAPTRSLALTKWLVNTSLESDRATAFQNEAVAQELNMGTVDANEGVAAFVERRDPTY
                     RGW"
     gene            complement(1203..2699)
                     /locus_tag="VNQ33_08005"
     CDS             complement(1203..2699)
                     /locus_tag="VNQ33_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ98087.1"
                     /translation="MPAAAPPDPTTAPFLPGTVAWAATRFGDRPAFVDPDGTEVGYAD
                     LHRRSDEVAAGLTRRGIGEGDVVALTLPSVTAWVVAYVAAAKVGAVTAGVNPRLTAVE
                     QAACLERVEPALVLATVDEVEALAVRGGTPDALAPGPDRPVAIVFTSGTTGIPKGAWF
                     TDRQLDAVTTYDVAHAVGTPTRLIPQYGSTQFAHVGFTTKLPWYLRLGTTTHLLARWR
                     PGDVLDLVERAGIETIGGVAPQVALLLRDPGFDQRDLSMVRLLVMGGAASPPALVQEA
                     RQRFEAAYSIRWSSTESGGVGTGTAPDAPDSEALHTVGRPRPGIGIQVRGADDLPLPH
                     GEVGAVWIRSEASMSGYWRDPEATAETLVAGWLRTGDLGSTDASGLLTLAGRQGEMFI
                     RGGYNVFPSEVSGVLASHPGVADAVVIPRPDDVLGEIGVAVVVPADPSAPPSLASLRE
                     HAQDRLAHHKLPEDVLVVDEIPLTAMQKVDRRRLSALVQEPEPPKPPI"
     gene            complement(2753..2911)
                     /locus_tag="VNQ33_08010"
     CDS             complement(2753..2911)
                     /locus_tag="VNQ33_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98088.1"
                     /translation="MRRRTRTAFAAIALAATLTTLGACGGRSPDGGNPGNPSADTTTT
                     TGTTIPSG"
     gene            3104..4324
                     /locus_tag="VNQ33_08015"
     CDS             3104..4324
                     /locus_tag="VNQ33_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWJ98089.1"
                     /translation="MRSPVILSADQARRIALGAQGFAEPAPKGMVDRRHGRKVLDRVG
                     LIQIDSVNVLVRSQELPLFARLGDHRRDLIPSMTKSGDLFEYWAHEASHIPVDLQPLL
                     RWRMDDAREGRGTWKGVAAIRHEEPALVQSVLDQVRERGPLTTGMIEGGGERGDDMWA
                     WSPGKRALEYLFWSGDLTARRGSNFERWYDLPEHVLPARVIEAATPVPTDAKKELLVV
                     GARSHGVGTARDLADYFRLNVPESRPLLDELVDEGRLLPAEVTGWKDKAYLHPGARRP
                     NNPKGRALLSPFDSLVWERARTERIWGFRYRIEIYVPKPKRVHGYYVLPFLLDGHLVA
                     RVDLKSDRKAGALLVQAAFAEDGVDRSRVARELAGELAALAAFLGLEGVEVRPDGDLA
                     PALGLAVEADGSPG"
     gene            complement(4608..5420)
                     /locus_tag="VNQ33_08020"
     CDS             complement(4608..5420)
                     /locus_tag="VNQ33_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWJ98090.1"
                     /translation="MPTDLTSFTTLKATQRGAVVWIWLDRPDKYHAFDKVMCDEIAGL
                     WRSLKTDDSVRSVVLTATGDKAFCTGIDRDFVPAEGGVEYDFSPFTYDDPGKYLGPKS
                     NDFWKPVVCAVNGMACGGAFYFLGEVDILIAADHATFFDPHVTYGMPAVYEPLLMLHR
                     GVPFGEILRMTLLGNHERLSAERALEIGLVSEVVPGADLEAAAAWLADTIASAPAGPM
                     QATLRTLWAGRELSRQQALGLGNTFLNLGMSEESLAEGQKAFGGERVKPRLR"
     gene            complement(5446..6696)
                     /locus_tag="VNQ33_08025"
     CDS             complement(5446..6696)
                     /locus_tag="VNQ33_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ98091.1"
                     /translation="MSELPKIISVDDHVVEPAHVWQEYLPQRFKADGPRIERRGVGAM
                     KHIGGGAYEQEFDPDGRPADCWVFGDLVYIHKRHVAAVGYSRDEMTMTPMTYDEMRPG
                     CYDPAARIADQEVNHVEASLCFPSFPRFCGQTFTEHPDRELGLACVEAYNDWMVEEWC
                     GDSDGALIPLIIVPLWDAELAAAEVRRNAARGVHAVTFSEVPSHLGLPSIHSGFWDPF
                     FAACEETETTINMHIGSSSRMPATSGDAPVAVAASLSFNNSMASLSDWLFSGNLVKFP
                     KLTLAYSEGQIGWLPYILERVDDVWREHRAWGGVKDLIPEPPSSYYYRNVYGCFFRDR
                     HGLVAIDEVGEDNITFETDYPHTDSTWPDTKQVAEKLVEGLTDEQIYKVMRGNAIEML
                     HLDLDRDVHTTMGLKRAAAIDLLG"
     gene            complement(6769..>7288)
                     /locus_tag="VNQ33_08030"
     CDS             complement(6769..>7288)
                     /locus_tag="VNQ33_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HWJ98092.1"
                     /translation="VFGLGGLLVGVALVVWLGSVALDGSSGMLGGGGGDTAPTTAVPI
                     ARSGAAIAIDPPADLADGAEVRVTGSGFPSGPVSITACLTHAPGGGGDRCDDSTGTVA
                     AAGTGGKLLATRAVPRVITVGGVAYDCAAAPGACSLRARPIGRPGDPGVTAGILFATG
                     RSPTDAEVPPTG"
BASE COUNT         1028 a         2398 c         2801 g         1061 t
ORIGIN      
        1 aggggatgca gggtgacgcc gctcagcgag tcgccgactc gatcttcggc gtgtgatccg
       61 gggcgcttgt gacctcgctg tgacctgaat cgggatttcg tgccatctcg cgcagatgcc
      121 caggctggtg acctgggcat ccttgcgact tcgttggctc gggggggagg actcgaaccc
      181 ccaacgactg gaccagaacc agctgtgtta ccgattacac cacccccgaa cgggcggacg
      241 atgactctag agcacctcgt cgccggggtg aaaacggttg ccctgacgcc agtcgtggtc
      301 cgtggtcacc agccccggta ggtggggtcg cggcgctcga cgaacgcggc cacgccctcg
      361 ttggcgtcga cggtgcccat gttcagctcc tgggcgacgg cctcgttctg gaacgccgtg
      421 gcccggtcgc tctcgagcga ggtgttcacg agccacttgg tgagggcgag cgagcgggtg
      481 ggggcgacgg cgaggcgggc ggcccacgcc tccacggcat cgtccagctc accggccggg
      541 acgaccttgt tgatcaggcc cagctcggcg gcctcggtgg ccgacaggtc gtcgccgaag
      601 aacaggagct ccttggcccg gcggacgccg acgatgcggg ggaggaggta ggcaccgccg
      661 ccgtcgggca cgaggccccg gcggacgaac acctcgatga accgcacgcc gtccgctgcc
      721 accaccaggt cgcaggcgag ggcgatgtgg gcgccgatgc ccgccgcagt gccgttgaca
      781 gcggcgatga ccggcttctc gcagtcgagg atggcggcca ccagggcctg ggcaccctcg
      841 cggatgagct tggcgacctc gccgacgccg cgcttggggg catcgtcggg gcgcggtgcc
      901 ggcggccggc tggcccgcag gtcggcgccg gtgcagaagg cgtcgccggt gtggccgagg
      961 acgacggctc ggacgccgag gtcggcgctg gcctcgccga gcagggcgat gacccggtcc
     1021 cgctggtcgg ggctgatggc gttcttcacg tcgggccggt tgagcgtgat ggaggcgatg
     1081 ccgcgctcgt cgacgcggtg ctggacctgc tcggtgaggg gaagctcgga cgacggcgtg
     1141 gacacggtgg gctccagggg tcagcggact gcgggcagcg tacgagttga cctgacgggt
     1201 cgtcagatcg gtggcttcgg tggttcaggc tcttggacca gcgcgctgag gcggcgccgg
     1261 tcgaccttct gcatcgccgt cagcgggatc tcgtcgacca ccaggacgtc ctcgggaagc
     1321 ttgtggtggg cgaggcggtc ctgggcgtgc tcccggaggc tggcgaggct gggcggtgcg
     1381 ctcggatcgg cgggcaccac gacggcgacg ccgatctcgc cgagcacgtc gtcgggccgg
     1441 gggatcacca ccgcatcggc gactccgggg tgggaggcaa ggacgccgga cacctcggag
     1501 gggaagacgt tgtagccgcc acggatgaac atctcgcctt ggcgtccggc cagggtcagc
     1561 aggcccgacg cgtcggtgga gccgaggtcg ccggtgcgca gccagccggc caccaacgtc
     1621 tcggccgtgg cctccggatc gcgccagtag ccggacatcg atgcctcgga ccggatccac
     1681 acagcgccga cctcgccatg gggaaggggc aggtcgtcgg cgccgcggac ctggatgccg
     1741 atgcccggcc gagggcggcc gacggtgtgc agcgcctcgc tgtcgggtgc atcgggcgcg
     1801 gtgccggtgc ccacgccgcc ggactccgtg gacgaccatc ggatcgagta cgccgcctcg
     1861 aagcgctggc gggcctcttg taccagtgcc ggcggcgatg ccgccccgcc catgaccagg
     1921 aggcgcacca tcgacaggtc gcgctggtcg aagccggggt cgcggagcag gagggcgacc
     1981 tgaggtgcga cgccaccgat cgtctcgatg ccggcccgtt ccaccaggtc gagcacgtcg
     2041 ccggggcgcc accgggcgag caggtgcgtc gtcgtcccca ggcgcaggta ccagggcagc
     2101 ttcgtcgtga acccgacgtg ggcgaactgg gtggagccgt actgcgggat cagccgcgtc
     2161 ggcgtgccga cggcgtgggc gacgtcgtac gtcgtcacgg cgtcgagctg ccggtcggtg
     2221 aaccacgccc ccttcgggat gccggtggtg cccgacgtga acacgatggc caccggccgg
     2281 tccgggccgg gcgcgagggc gtcaggcgtc ccaccccgca cggcgagggc ctccacctcg
     2341 tcgaccgtgg cgaggacgag cgccggttcg acgcgctcga ggcaggcggc ctgttccacc
     2401 gcggtcagcc gggggttgac gccggcggtg acggcgccga ccttggcggc ggcgacgtag
     2461 gcgacgaccc acgccgtgac tgacgggagg gtgagggcga cgacgtcgcc ctcaccgatg
     2521 ccccggcggg tgaggccggc ggccacctcg tcgctgcgtc ggtgcaggtc ggcgtagccg
     2581 acctcggtgc cgtcgggatc gacgaacgcc ggacggtcac cgaagcgggt ggcggcccag
     2641 gcgacggttc ctggcaggaa cggcgcggtc gtcgggtccg gcggagcggc agcgggcacg
     2701 gtcggtgacc ctagcgacgg gccgtgctca ggtggcggga ccggcgatcg ggtcagccgc
     2761 tggggatcgt ggtgccggtg gtggtcgtcg tgtccgccga tgggttcccg gggttgccgc
     2821 cgtcggggga gcggccgccg cacgcgccga gcgtggtcaa ggtggcggcg agggcgatcg
     2881 cagcgaaggc ggttcgagtg cggcggcgca tgggtcagat cctccagaca ggacccttcc
     2941 atcctgcccg accggccggg tccggcagca gccggctgct gccggttctt gttccagagc
     3001 ggcgttcgtg ggcgacgcga cctggaacga gagcggatcg aacccctccg tgtccgaact
     3061 ccgccaccgg taccggcgaa gatccgacat gatggtcggc cccatgcgct cgcccgtcat
     3121 cctctccgcc gatcaggcgc ggcgcatcgc gctgggcgcg cagggcttcg ccgagccggc
     3181 cccgaagggg atggtcgacc gccgccacgg tcgcaaggtg ctggaccggg tggggctcat
     3241 ccagatcgac tcggtcaacg tcttggtgcg ctcgcaggag cttccgctgt tcgcccggct
     3301 cggcgaccac cggcgggacc tgatcccgtc gatgaccaag tccggcgact tgttcgagta
     3361 ctgggcgcac gaggcttcgc acatcccggt cgacctccag ccgctcctgc gctggcgcat
     3421 ggacgatgcc cgcgaggggc gcggcacgtg gaagggcgtg gccgccatcc gccacgagga
     3481 gccggcgctg gtgcagtcgg tcctcgacca ggtccgcgag cgcggcccgc tgaccacggg
     3541 catgatcgag ggcggcggcg agcgcggcga cgacatgtgg gcgtggagcc ccgggaagcg
     3601 ggcgctcgag tacctgttct ggagcggcga tctcaccgcc aggaggggga gcaacttcga
     3661 gcgctggtac gacctgcccg agcacgtgct gccggcccgg gtgatcgagg ccgccacccc
     3721 ggtgccaacg gacgccaaga aggagctgct cgtcgtcggt gcccggtccc acggcgtcgg
     3781 cacggcccgc gacctggccg actacttccg cctcaacgtc cccgagtccc gaccgctcct
     3841 cgacgagctg gtcgacgagg gccgcctcct tcccgccgag gtgacgggtt ggaaggacaa
     3901 ggcgtacctc caccccggcg cccggcggcc caacaacccg aagggccggg cgctgctgtc
     3961 gcccttcgac tcgctggtct gggagcgggc ccggacggag cgcatctggg gcttccgcta
     4021 ccgcatcgag atctacgtgc cgaagccgaa gcgggtgcac ggctactacg tgctcccgtt
     4081 cctcctcgac ggccacctcg ttgcccgcgt cgacctcaag tccgaccgca aggccggggc
     4141 cctcctggtc caggctgcct tcgccgagga cggcgtcgac cggtcgcggg tggcgaggga
     4201 actggccggt gagctggccg cgctcgccgc cttcctcggc ttggagggtg tcgaggtcag
     4261 gcccgacggc gacctcgccc cggccctcgg ccttgccgtc gaggctgatg gatcgcccgg
     4321 ctgacgaact ggctgacaga ccggctgacc atccgaccgg ctgaacatcc gaccggccgg
     4381 ccatccggcc atccggatct tggaggatcg gtgtcgctca gcgaccacga tcctccaagt
     4441 tcgcccgtgg tcccgtcact catcgaccaa gttggaggat gagggcggta gacgaccacg
     4501 atcctccaag ttggaggatg aaggcggggt ggggcgtcgg gcgtgagggc gtcgggggtg
     4561 agggcgtcgg gcgtcgggca tgagggcggg ggaacggcga cggtgggtca gcggaggcgg
     4621 ggtttgaccc gttcgccgcc gaaggccttc tggccttcgg cgagggattc ctcggacatg
     4681 ccgaggttga ggaacgtgtt gccgaggccg agggcctgct ggcggctgag ctcgcggccg
     4741 gcccacaggg tgcggagggt ggcttgcatc gggccggcgg gggccgaggc gatggtgtcg
     4801 gcgagccagg cggctgccgc ttcgaggtcg gcgcccggga cgacttcgga gacgaggccg
     4861 atctccaggg cccgctcggc ggataggcgc tcgtggttgc cgagcagcgt catgcgcagg
     4921 atctcgccga agggcacccc gcggtggagc atcagcagcg gctcgtagac cgccggcatg
     4981 ccgtaggtga cgtgcgggtc gaagaacgtg gcgtgatccg cggcgatgag gatgtcgacc
     5041 tcgccgagga agtagaacgc gccgccgcac gccatgccgt tgacggcgca caccaccggc
     5101 ttccagaagt cgttgctctt ggggccgagg tacttgccgg gatcgtcgta ggtgaagggg
     5161 gagaagtcgt actcgacccc gccctccgcg ggcacgaagt cccggtcgat gcccgtgcag
     5221 aacgccttgt cgccggtggc cgtgagcacg accgagcgga cggagtcgtc ggtcttgagg
     5281 gagcgccaca gtcccgcgat ctcgtcgcac atgaccttgt cgaaggcgtg gtacttgtcg
     5341 ggccggtcca gccagatcca cacgaccgcg ccgcgctggg tggccttgag ggtggtgaac
     5401 gacgtcaggt cggtgggcat ggcgtgaacg tacctggcgc cgtgatcagc cgaggaggtc
     5461 gatggccgca gcgcgcttga ggcccatcgt ggtgtgcacg tcgcggtcga ggtcgaggtg
     5521 cagcatctcg atcgcgttgc cgcgcatcac cttgtagatc tgctcgtcgg tgaggccctc
     5581 gacgagcttc tccgccacct gcttggtgtc gggccaggtg gagtcggtgt gggggtagtc
     5641 ggtctcgaac gtgatgttgt cctcgccgac ctcgtcgatg gccaccagcc cgtggcggtc
     5701 gcggaagaag cagccgtaga cgttgcggta gtagtagctc gacggcggct cggggatgag
     5761 atccttgacc ccgccccacg cccggtgctc gcgccacacg tcgtcgaccc gttcgaggat
     5821 gtagggcagc cagccgatct ggccctcgga gtaggcgagg gtgagcttgg ggaacttcac
     5881 gaggttgccg ctgaagagcc agtcgctcag cgacgccatc gagttgttga acgacagcga
     5941 ggcggcgacg gcgacggggg cgtcgcccga ggtggcaggc atgcgcgacg acgagccgat
     6001 gtgcatgttg atggtggtct cggtctcctc gcaggcggcg aagaacgggt cccagaagcc
     6061 ggagtggatc gaggggaggc cgaggtgcga ggggacctcg gagaaggtga cggcgtggac
     6121 gccgcgggcg gcgttgcggc gcacctcggc ggcggccagc tcggcatccc acagggggac
     6181 gatgatcagc gggatgaggg cgccgtcgga gtcgccgcac cactcctcga ccatccagtc
     6241 gttgtaggcc tcgacgcagg cgaggcccag ctcccggtcg gggtgctcgg tgaaggtctg
     6301 gccgcagaac cgggggaacg acgggaagca cagcgacgct tccacgtggt tgacctcctg
     6361 gtccgcgatg cgcgccgcgg ggtcgtagca acccgggcgc atctcgtcgt aggtcatcgg
     6421 tgtcatggtc atctcgtcgc gggagtaccc gaccgcggcg acgtggcgct tgtggatgta
     6481 gacgaggtcg ccgaacaccc agcagtcggc cgggcggccg tcgggatcga actcctgctc
     6541 gtaggcgccg ccgccgatgt gcttcatggc gccgacaccc cggcgctcga tgcgggggcc
     6601 gtcggccttg aacctctgcg ggaggtactc ctgccagacg tgggctggct cgaccacgtg
     6661 gtcgtcgacg ctgatgatct tgggcagttc ggacacggcc cctcccggtt tctgacagac
     6721 catcagatcg tagccgagac gtctgcggcg ccgtctggct gtgccggatc aaccggtggg
     6781 cggcacctcg gcatcggtgg gcgagcgccc ggtggcgaac aggatcccgg cggtgacgcc
     6841 gggatcgccg ggtcggccga tgggcctcgc tcggagcgag cacgcacccg gggcggcggc
     6901 gcagtcgtag gccacgccgc cgacggtgat cacgcggggc acggcccgag tggcgagcag
     6961 cttcccgccg gtcccggccg ccgccaccgt gcccgtgctg tcgtcgcagc ggtcaccgcc
     7021 tcctcctggc gcatgggtga ggcacgcggt gatgctgacc gggccggacg ggaagccgga
     7081 tccggtgacg cggacctcgg cgccgtcggc gaggtcggcg ggcgggtcga tggcgatggc
     7141 cgcgccgctc ctggcgatcg ggaccgcggt ggtgggtgcc gtgtcgcccc cacctccgcc
     7201 gagcatgccg gacgagccgt cgagggcgac cgagcccagc cagacgacga gggcgacgcc
     7261 caccagcagg ccgccgaggc cgaacaca
//
LOCUS       DATMFS010000425         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_317924, whole genome shotgun sequence.
ACCESSION   DATMFS010000425 DATMFS010000000
VERSION     DATMFS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317924"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VNQ33_08035"
     CDS             complement(<1..441)
                     /locus_tag="VNQ33_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98093.1"
                     /translation="MTFAYRTAAGRGGLGPPPPKRPRRGRSYSGWIGIGVRRITGADV
                     LVFLGVFALLYVAIRVGRDAAVSFTPQDVSTIDTSASRLPYDAARSLLRMFLALGASY
                     AFTLAYGYVAARNRRAEKVLIPLLDILQSVPILGFLSLTITAFIA"
     gene            complement(443..1786)
                     /locus_tag="VNQ33_08040"
     CDS             complement(443..1786)
                     /locus_tag="VNQ33_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HWJ98094.1"
                     /translation="MTTTIEERRPRALPGELLIEADSVTKTFSTPEGELPVLDGVSLQ
                     VHAGEVVALLGKSGSGKSTLLRAIAGLLTPTAGTISHHGQRLVGPNPGTAMVFQSFAL
                     LPWLTVRQNVELGLEAKGVDRARRRDRAERAIDLIGLDGFEDAYPKELSGGMRQRVGF
                     ARALVVEPEVLLMDEPFSALDVLTAENLRGELMELWGSDEFPIESIVLVTHNIEEAVL
                     LADRVVILGSNPGRIKLELPIGLARPRDRHDPSFEQLVNRIYEVMTGRLPADVVAPMA
                     ATPASVPLPHATVDGLSGLAELLAGRGRQPIADTAEQLTFEVDDLLPVVDALELLGFA
                     SVEHGSVELTEPGRTFAGADIQASKQMFARAVLQHAPLVRAIANDVAREADGTLKARV
                     FRDVLRRSFSDGETQVQLDVASDWGRYAEQFSYDADHDEFLTDPDRDRVLLEGDG"
     gene            complement(2261..2908)
                     /locus_tag="VNQ33_08045"
     CDS             complement(2261..2908)
                     /locus_tag="VNQ33_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ98095.1"
                     /translation="MADKEATSIDRRRERSRRTRRTVVAAATDLFVRDGYGATSIASV
                     ASRAGVAVQTVYATFGTKAAILDAALDVAIAGDDEDVAVNDRDWMHTVFHAPTGTERL
                     AAYAAACRRIHANAADVFRVVEMASTSDPAVVELAGIVADRRRRGAASVVAAVVEVAA
                     LRPGLSPGEAVDVLWLLNSPGTFHLLVREGGWSLDHFEHWLAGALQRELLAPAVG"
     gene            2957..3427
                     /locus_tag="VNQ33_08050"
     CDS             2957..3427
                     /locus_tag="VNQ33_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ98096.1"
                     /translation="MLMAMTPTSDPRWFAGTLLRVLADAERTDGQLTVMEQRAPRHFS
                     PPRHVHHREDTALVVLDGRLTVEVGDERRVVGTGEMVWLPREVPHTFRVDSDEAHLIE
                     LATPAGIEGFHLDASDPAPSAVLPPPGQPDISRLQAAIEPYGADIIGPPLFAAD"
     gene            complement(3439..>3899)
                     /locus_tag="VNQ33_08055"
     CDS             complement(3439..>3899)
                     /locus_tag="VNQ33_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWJ98097.1"
                     /translation="SSPVADALAGLRANEARYFANKYKHAFVTEPASKAKKTVAYVAK
                     ILKDERDLVIGSKPLEACEFEVETTTVEELERAIPQEIVRWAYVFYENGLSVNVLYTL
                     TDGGKRAVGFKLSDGMEVPEELAEKFKFARQKSKLAGTIRGSYFVIKGEY"
BASE COUNT          605 a         1388 c         1337 g          569 t
ORIGIN      
        1 ggcgatgaag gcggtgatcg tgagcgacag gaacccgagg atcggcacgg actgcaagat
       61 gtcgaggagc gggatcagca ccttctcggc ccggcggttc ctggcggcga cgtagccgta
      121 ggcgagcgtg aacgcgtagg acgctccgag cgcgaggaac atgcgcagca ggctgcgcgc
      181 cgcgtcgtag ggcaggcggc tggcggaggt gtcgatcgtc gagacgtctt ggggggtgaa
      241 gctgacggcg gcgtcgcgcc ccacccggat cgccacgtac aggagggcga acacgccgag
      301 gaacacgagg acatcggcgc cggtgatccg gcggacgccg atgccgatcc acccgctgta
      361 ggagcggccg cggcgcgggc gcttgggcgg tggcggtccg agcccgcctc gtccggcggc
      421 ggtgcggtag gcgaaggtca tctcacccat ctccttcgag caggacgcgg tcgcggtcgg
      481 ggtccgtcag gaactcgtcg tgatcggcgt cgtaggagaa ctgctcggcg tagcggcccc
      541 agtcgctcgc cacatcgagc tgcacctggg tctcgccgtc gctgaacgag cggcgcagca
      601 cgtcgcggaa gacccgggcc ttgagcgtcc cgtccgcctc gcgggccacg tcgttggcga
      661 tggcccgcac caggggcgcg tgctggagga cggcccgggc gaacatctgc ttcgacgcct
      721 ggatgtcggc gccggcgaag gtgcggccgg gctcggtgag ctccacgctg ccgtgctcga
      781 cggaggcgaa gcccagcagc tcgagcgcgt cgacgaccgg gaggaggtcg tccacctcga
      841 acgtgagctg ctcggcggtg tcggcgatgg gctggcggcc tcgccccgcc aacagctccg
      901 cgaggcccga caggccgtcg accgtggcgt ggggcagcgg gaccgacgcc ggtgtcgccg
      961 ccatcggcgc gacgacatcg gcggggaggc gacccgtcat cacctcgtag atgcggttca
     1021 cgagctgctc gaagctggga tcgtggcggt cacgcggccg ggcgaggccg atcggcagct
     1081 ccagcttgat ccgccccggg ttcgatccca agatgaccac ccggtcggcg agcaggaccg
     1141 cctcttcgat gttgtgggtc accaagacga tcgactcgat ggggaactcg tcgctgcccc
     1201 acagctccat gagctcgccg cgcaggttct cggccgtgag cacgtccagc gcggagaacg
     1261 gctcgtccat gagcagcacc tcgggctcga ccaccagcgc gcgggcgaag cccacccgct
     1321 ggcgcatccc gcccgacagc tccttggggt aggcgtcttc gaacccgtcc aagccgatga
     1381 ggtcgatggc gcgctcggcc cggtcccggc gccgggcgcg gtccaccccc ttcgcctcca
     1441 gaccgagctc gacgttctgg cgcaccgtca gccacggcag cagggcgaac gactggaaca
     1501 ccatggccgt gcccgggttc ggtcctacga gccgctggcc gtggtgggag atggtgccgg
     1561 cggtgggggt cagcagcccg gcgatggcgc gcagcagcgt ggacttcccg ctgcccgact
     1621 tgccgagcag cgccacgacc tcaccggcgt ggacctgcag cgacaccccg tcgaggacgg
     1681 gaagctcgcc ttctggggtc gagaacgtct tcgtcaccga atcggcttcg atcaacagct
     1741 cacccggcag cgcgcgcggg cgacgctctt cgatggtcgt cgtcatggtc ttctcctgca
     1801 tcgtccggcc acgcgcgtgc gcggccgcgg aacgacgagg actgcagttc gcaggggcgc
     1861 ccggcccgaa cgggcgccgt caggcctgga gcagaccggc gaccaccgaa gggaccgtgc
     1921 gcaagcaact actgccgtcg ctcatcgatc cacctcctcg gtcatcagcc aaactcacac
     1981 ctcgatacga ggccctggat gtctacccga accacccgga caacgccaac cccttcctgc
     2041 tcaggcccga cgcgcgccca tgccgaggac cccctcgacg acctcgtgcg gacgaggtcc
     2101 agggccgtcg tcttcaggtg ggatggacac gagccgggct ctcgaacgac gggccgcggg
     2161 tgccgacggt cctcgtcggc gtgctcaacg ctgctcaccg tccggctggc cgggggcatc
     2221 cgccgatgga gcggcggtcc cggtccgagg gcgctcgtcg tcaaccaacg gccggagcga
     2281 gcagctctcg ctggagggct ccagccagcc agtgctcgaa gtgatcgagg gaccacccac
     2341 cctcgcgcac caggaggtgg aaggtccccg ggctgttgag cagccacagc acatcgaccg
     2401 cctcgcccgg tgagagcccg ggccgaagcg ccgcaacctc caccacggcg gcgaccaccg
     2461 acgctgcgcc tcggcgccga cgatccgcca cgatccccgc cagttcgacg accgcaggat
     2521 cggaggtgga tgccatctcc accacgcgga acacatcggc ggcgttggca tggatccgcc
     2581 ggcaggcagc ggcgtaggcc gccagccgct cggtccccgt cggcgcgtgg aagacggtgt
     2641 gcatccagtc gcggtcgttc accgccacgt cctcgtcgtc gcctgcgatc gccacgtcca
     2701 gcgcggcatc gaggatcgcc gccttggtgc cgaacgtggc gtacaccgtc tggaccgcca
     2761 cgccagcccg gctggcgacc gaagcgatcg acgtggcccc gtagccgtca cggacgaaca
     2821 ggtccgtggc ggcggcaacg accgtccgcc gggtgcgccg cgaccgttcc cgacgtcggt
     2881 cgatcgacgt cgcctccttg tcagccacga gccaggagta tatatctgta gtgtcactct
     2941 aaatagtttg gagctcgtgc tcatggccat gacccccacc tccgaccccc gctggttcgc
     3001 cggcaccctg ctgcgggtcc tcgccgacgc cgagcgcacc gacggccagc tgaccgtgat
     3061 ggagcagcgg gctcctcgcc acttctcgcc gccgcgccac gtgcaccacc gcgaggacac
     3121 ggccctcgtc gtcctcgatg gaaggctgac cgtcgaggtc ggcgacgagc gacgagtcgt
     3181 cggaacagga gagatggtgt ggctcccgcg tgaggtgccc cacacctttc gggtcgacag
     3241 cgacgaagct cacctgatcg agctggccac ccccgccggc atcgaagggt tccacctcga
     3301 cgccagcgac cccgcgcctt cggccgtgct ccccccaccg ggccaacccg acatctcgcg
     3361 gctgcaagcc gcgatcgaac cgtatggagc cgacatcatc ggacctcccc tgttcgccgc
     3421 cgactgagga cgctgcggtc agtactcgcc cttgatgacg aagtagctgc cgcggatggt
     3481 gccggcgagc ttggacttct ggcgggcgaa cttgaacttc tccgccagct cctcgggcac
     3541 ctccatgccg tcggacagct tgaacccgac ggcccgcttg ccgccgtcgg tcagcgtgta
     3601 cagcacgttg accgacaggc cgttctcgta gaacacgtac gcccagcgga cgatctcctg
     3661 cgggatggcg cgctcgagct cttcgaccgt ggtggtctcc acctcgaact cgcacgcctc
     3721 gagcggcttc gacccgatga cgaggtcgcg ctcgtccttc aggatcttgg cgacgtaggc
     3781 gacggtcttc ttggccttcg acgccggttc ggtgacaaac gcgtgcttgt acttgttggc
     3841 gaagtagcgg gcctcgttgg ctcgcagccc ggcgagggcg tcggccaccg gcgacgact
//
LOCUS       DATMFS010000426         4031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_84816, whole genome shotgun sequence.
ACCESSION   DATMFS010000426 DATMFS010000000
VERSION     DATMFS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84816"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VNQ33_08060"
     CDS             complement(<1..397)
                     /locus_tag="VNQ33_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWJ98098.1"
                     /translation="MTDSATEAEVTRAGSATSPRSFAEFYETEAESIHRALRSTVANP
                     VVAREAVDEAMARAYERWAVVGGYDAPAGWVYRTALNWSISWWRKHRREQAVPTEETG
                     HGGAGEVDPAGALAIEALRRLPLDQRSVVV"
     gene            589..1236
                     /locus_tag="VNQ33_08065"
     CDS             589..1236
                     /locus_tag="VNQ33_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWJ98099.1"
                     /translation="MAEINLKATTGRELGSGPSKRIRAEGNVPGVVYGLDADPIPVTV
                     EWRPLREALTTDAGLNALIDLEVDGDVALTVVKELQRHPIRGNVLHIDFLRVSADAEI
                     TVEVPIVLEGEAKSVLSDGGTVDQLLYALSINAKPADIPNEVTVDISGLEINGSIHVS
                     DIALPAGVSTDVDPEESVVAGNQAQVVEEPAAGEGEAEGGDAAAADAPAEGDAEG"
     gene            1220..1873
                     /gene="pth"
                     /locus_tag="VNQ33_08070"
     CDS             1220..1873
                     /gene="pth"
                     /locus_tag="VNQ33_08070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWJ98100.1"
                     /translation="MTPRADALVGPDAMAFRRSPERRGTPADLVVLGLGNPGATYAHS
                     RHNVGADVVELLAERHGGRLKSSTKERSLVAEVRIGTARVVLAFPQTFYNEAGSAARL
                     LLERHGIDDLHHLVVVHDEMDLPPGKLKVKIGGGLAGNNGLKSIKAHLKTDDFVRVRV
                     GIGKPPGRQEGVDHVLRRPGKAEKAELDIAMQEAADAVEAILADGAELAQTRFNARS"
     gene            complement(1895..2761)
                     /locus_tag="VNQ33_08075"
     CDS             complement(1895..2761)
                     /locus_tag="VNQ33_08075"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HWJ98101.1"
                     /translation="MGRWARSKQLASASWGVLKQDKELMALPLISGLASLVIGATFLF
                     PLYLSSETKDVAGSTTFEMKPIGYVVLFLMYLVLAYIAIFFKTALLCGADERMRGGEP
                     SLGTALSGAASRAGKILPWAIVTATVSVILRSLEERAGILGRIVIGLVGMAWAVVTFL
                     VLPIVVFENVGVGTAIKRSAEMLKRTWGENLIVNLGIGLISMVLTLPAIAVVIGSFFT
                     GSAPVIVGAVVLAAVWIIGVSCWSSAMTAVFQLALYRYATQTPLPSEFAQVNMAEAFG
                     PKKASRLGFGGR"
     gene            2936..>4031
                     /locus_tag="VNQ33_08080"
     CDS             2936..>4031
                     /locus_tag="VNQ33_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWJ98102.1"
                     /translation="MALTDLPALLRDEPALTGVLGRRAALLAVPEPARPLTIAGLATV
                     GSRRPILVAVPTTAEAERLVHDLGMFLGPGAAELFPAWETLPFERVSPNIETMGRRLR
                     TIWRLRGGSAEAPQVVVAPVRALVQRLGPHVDETEPLVVRPGERRDQAALVAWLGEHG
                     YRREGQVEHRGEFAVRGSIVDVFGSTADVPVRIDLWGDEVDRLTAFSVSDQRSTGDLD
                     GIEVFPCRELLPTGDVRERASALIASEPWGREQWERLVEGQLFDGMESWLPWLTDDHR
                     TIVDLLPPESLVLVAEPRRLRDRAADLLAEEADLASSLARTWEVGDDRAFPQLHAAFA
                     DLFADSDLPVWSVTVTPDGPDVATVAAMGWN"
BASE COUNT          587 a         1505 c         1395 g          544 t
ORIGIN      
        1 agacgaccac ggaccgttga tcgagaggca ggcggcggag ggcctcgatg gcgagggcgc
       61 ctgcgggatc gacctcgccc gccccgccgt ggccggtctc ctcggtgggc acggcctgct
      121 cgcggcggtg cttgcgccac cacgagatcg accagttcag ggcggtgcgg tacacccacc
      181 cggcgggggc gtcgtagcct cccacgacag cccagcgctc gtaggcccgg gccatcgcct
      241 cgtcgacggc ctcgcgggcc accaccgggt tggcgacggt ggagcgcagc gcccggtgga
      301 tcgactcggc ttcggtctcg tagaactcgg cgaacgagcg cggcgaggtc gccgagccgg
      361 ctcgggtcac ctcggcctcc gtcgcgctgt ccgtcatcac gtcagcttca cgcctcagcg
      421 cgccgaatgg ttcccagacc ggtcgagcgc ccgtgcccga cgtcgcaccg gccgccgccg
      481 ttcccaagcg gctccccgat gagggccgtc ggcggccgga cccctaggct ggcgagccgt
      541 ctgacaaccg aaagcgctcc ggcgcgatct ctgaagtagg agctaccgat ggctgagatc
      601 aacctgaagg ccaccaccgg ccgtgaactg ggctcgggcc cgtccaagcg gatccgggcc
      661 gagggcaacg tccccggcgt cgtgtacggc ctcgatgccg acccgatccc cgtcaccgtg
      721 gagtggcgcc cgctgcgcga ggccctcacc accgatgccg gcctcaacgc cctcatcgac
      781 ctcgaggtcg acggcgacgt cgccctcacc gtggtcaagg agctgcagcg ccacccgatc
      841 cgcggcaacg tgctgcacat cgacttcctg cgggtcagcg ccgacgccga gatcaccgtc
      901 gaggtgccga tcgtgctcga gggcgaggcc aagtccgtcc tgtccgacgg cggcaccgtc
      961 gaccagctgc tctacgcgct gtcgatcaac gccaagccgg ccgacatccc caacgaggtc
     1021 accgtcgaca tctccggcct ggagatcaac ggctcgatcc acgtgtccga catcgcgctc
     1081 cccgccggcg tctccaccga cgtcgacccc gaggagtcgg tcgtggccgg caaccaggcg
     1141 caggtcgtcg aggagcccgc tgctggcgag ggcgaggccg aaggcggcga cgccgccgcc
     1201 gccgatgctc cggccgaggg tgacgccgag ggctgacgcc ctcgtcgggc ctgacgccat
     1261 ggcgttccga cgttcgccgg aacggcgagg cacccccgca gatctggtgg tgctcggcct
     1321 cgggaacccc ggggccacct acgcccactc gcgccacaac gtgggggccg acgtggtcga
     1381 gctcctcgcc gagcgccacg gcggccgctt gaagtcctcg accaaggagc gctcgctcgt
     1441 ggccgaggtg cgcatcggga cggcccgcgt cgtcttggcc ttcccgcaga ccttctacaa
     1501 cgaggccggc agcgcggcgc gcctgctgct cgagcgccac ggcatcgacg acctccacca
     1561 tctcgtcgtc gtccacgacg agatggacct gccgcccgga aagctcaagg tgaagatcgg
     1621 cggtgggctc gcgggcaaca acgggctcaa gtcgatcaag gcccacctga agaccgacga
     1681 cttcgtgcgc gtgcgcgtcg gcatcggcaa gccgcccggc cgccaggagg gcgtcgacca
     1741 cgtcctgcgc cgtcccggca aggccgagaa ggccgagctc gacatcgcca tgcaggaggc
     1801 ggccgacgcc gtcgaggcga tcctggcgga cggcgccgag ctggcccaga cccgcttcaa
     1861 cgcccgctcg tagcgggccg cggccgcgcc tcgttcagcg gccgccgaag ccgagccggc
     1921 tggccttctt cggcccgaac gcctcggcca tgttcacctg ggcgaactcg gacggcagcg
     1981 gcgtctgggt ggcgtagcgg tacagggcca gttggaacac ggcggtcatg gccgaggacc
     2041 agcagctcac gccgatgatc cagacggcgg cgaggacgac cgccccgacg atgaccggag
     2101 ccgagccggt gaagaacgaa ccgatgacca cggcgatggc cggcagcgtg aggaccatcg
     2161 agatgaggcc gatgccgagg ttgacgatca ggttctcgcc ccaggtgcgc ttcagcatct
     2221 cggccgagcg cttgatggcg gtgccgacgc cgacgttctc gaacaccacg atgggcagca
     2281 ccaagaacgt gaccacggcc caggccatgc ccaccaggcc gatcacgatg cggccgagga
     2341 tgccggcgcg ctcctcgagc gagcggagga tgaccgacac cgtggcggtg acgatggccc
     2401 aggggaggat cttcccggcc cggctcgccg ccccggacaa ggcggtgccg agcgacggct
     2461 cgccgccccg catgcgctcg tcggcgccgc agagcagggc cgtcttgaag aagatcgcga
     2521 tgtaggcgag cacgaggtac atcaagaaca gcacgacgta gccgatcggc ttcatctcga
     2581 acgtcgtgga accggcgacg tctttcgtct ccgagctcag gtacagcggg aacaggaacg
     2641 tcgcgccgat gacgagcgat gcgaggcccg agatcagggg cagcgccatc agctccttgt
     2701 cctgcttcag cacgccccag gaggcgctcg ccagttgctt cgatcgtgcc caacgtccca
     2761 tgcccgctcc tatcggtcgg cccggctcca gtctgcacct ctttcccgga ccgtgcgcgc
     2821 caccgggtgg gggagcgggt ccgccggtcg gtagcgtggc tccgcttcgc gacccccggt
     2881 gccctcgcat cggcggcgcg ctggcgcgcg ctccccacca cgaaggaccc ggcccatggc
     2941 gctcaccgat cttcccgccc tcctgcgcga cgaacccgcg ctcacgggcg tgctcggtcg
     3001 ccgtgcggcc ctcctggcgg taccggagcc ggctcggccc ctcaccatcg ctggtctcgc
     3061 caccgtcggg agccggcgcc cgatcctggt ggccgtcccc accacggccg aggccgagcg
     3121 cctggtccac gacctgggca tgttcctcgg ccccggtgcc gcggagctgt tcccggcctg
     3181 ggagacgctg ccgttcgagc gggtcagccc caacatcgag acgatgggcc gccgacttcg
     3241 cacgatctgg cggctgcggg gcggatcggc cgaggcgcca caggtcgtcg tcgcgccggt
     3301 gcgggccctg gtccagcgcc tcggccccca cgtcgacgag accgagcccc tggtcgtccg
     3361 ccccggcgag cggcgcgacc aggccgccct cgtggcctgg ctgggcgagc acggctaccg
     3421 tcgagagggc caggtcgagc accgcggcga gttcgccgtg cgcggctcga tcgtcgacgt
     3481 gttcggctcc accgccgacg tgccggtgcg catcgacctg tggggtgacg aggtcgaccg
     3541 cctgaccgcg ttctcggtca gcgaccagcg ctccaccggc gatctcgacg gcatcgaggt
     3601 gttcccctgc cgggagctgc tgcccaccgg cgacgtgcgc gagcgggcgt cggccctcat
     3661 cgccagcgag ccgtggggtc gggagcagtg ggagcggctg gtggagggcc agctgttcga
     3721 cggcatggag agctggctgc cgtggctcac cgacgaccac cgcaccatcg tcgacctgct
     3781 cccgcccgag tccctggtgc tggtggccga gccccgccgc ctgcgcgacc gcgccgccga
     3841 cctgctggct gaagaggccg acttggcctc gtcgctcgcc cgcacctggg aggtgggcga
     3901 cgaccgggcc ttcccgcagc tccacgccgc cttcgccgac ctgttcgccg actccgacct
     3961 gcccgtctgg tcggtcaccg tcacccccga cggacccgat gtggcgaccg tggccgccat
     4021 gggctggaac c
//
LOCUS       DATMFS010000427         3811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_246266, whole genome shotgun sequence.
ACCESSION   DATMFS010000427 DATMFS010000000
VERSION     DATMFS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246266"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VNQ33_08085"
     CDS             <1..1046
                     /locus_tag="VNQ33_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWJ98103.1"
                     /translation="PPLSLTTADLVAGRGLLEPHGLPALREALARVQTAHSRPTEASQ
                     IHVTHGAHHAIGLAIAAIVPAGAAIALEVPSYPGILDLIDHHRARAVGIPDGPGGPDP
                     EGLARVLATERPAAVYLQTGVHNPTGHVLSAARRRAIADVLDAHGDAVVIEDNTLADL
                     VYTGRPRPSMAEACRIAPVVTVESLSKTAWAALRIGWLRATGVVSERIGGVRLATDLG
                     PSVPSQLLALQLLPHLPQMAEDRRRELEAATVHAAARIREELPAWSLEPPAGSSALWP
                     RLPVPDATPFVALARRHGVHVTPGSVHVLSDAPDPHLRFCVDRPRGHVDEGLDRLVAA
                     WHDASSRSSRALA"
     gene            1178..2002
                     /locus_tag="VNQ33_08090"
     CDS             1178..2002
                     /locus_tag="VNQ33_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWJ98104.1"
                     /translation="MLPAAIPVDDPADPRIEEFLGLRDHELRRRRESPGGDLAGIFIA
                     EGDLVVERAVRAGYRLRSVLVDATRSQPLPVAVGPDVPVFAAGEEVVLRITGMAMHRG
                     LIASLDRGPDRDLAEVLADATRAVVLENVNNPTNLGVIARSAMGLGADALLLDPSCCD
                     PLYRRASRVAMGEVFALPWARTKRFPEGIDQVTDAGFSLLALTPDPAAEPIDDVVLAP
                     DEKAALVLGAEGPGLTTETMARVGRRVRIPLHRGVDSLNVGAAAAIACYVLGRGRP"
     gene            2132..2776
                     /locus_tag="VNQ33_08095"
     CDS             2132..2776
                     /locus_tag="VNQ33_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98105.1"
                     /translation="MSDGAGIDATGGDADAHVVLTALRTTGAKGLSVTISVVHRTVEL
                     LCVAAGATGLVALVLGVWAWRNTLPGAGIAFVCGGVTLAVAVYVLIRVGALAAAIKHP
                     AETLAQAQDLVLRAKGSPELHQLAGAVRTRQSRKAAKAAGVGRARRALNSGRLISRVI
                     GLASPDPERHRYLVPFTPDRLKDLWLAILVGLWAWLVSAAVASLAFLSLAIQAL"
     gene            complement(2941..3318)
                     /locus_tag="VNQ33_08100"
     CDS             complement(2941..3318)
                     /locus_tag="VNQ33_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98106.1"
                     /translation="MSSLLTTGEPTEHPRQPPKPFQAMMEVMRHVRFDVATLTMEGIP
                     VVLKAAYLLVAEQEGLAELQWECLAYSLDATSIPQSTYAVDITTLDGRDFAGRAVVVR
                     SLDGAHVLRGDGPLAGITDADLS"
     gene            3302..>3811
                     /locus_tag="VNQ33_08105"
     CDS             3302..>3811
                     /locus_tag="VNQ33_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWJ98107.1"
                     /translation="MSSDDTTPYPKVPSRADYPAIERRILEFWAADGTFQASIDQRPA
                     DDEYVFYDGPPFANGLPHYGHLLTGYVKDVVPRYQTMRGHRVARRFGWDTHGLPAEME
                     VEKEIGIAGHKAINEYGIEKFNEHCRTSVLRYTAEWERYVNRQARWVDFQDDYKTMDL
                     GFMESVIWAF"
BASE COUNT          499 a         1492 c         1314 g          506 t
ORIGIN      
        1 tcccgccgct gtcgctcacc accgccgacc tcgtcgccgg gcgcggcctg ctcgaacccc
       61 acgggctgcc ggcgctgcga gaagcgctgg cgcgcgtcca gaccgcccac agccggccca
      121 cggaggcgag ccagatccac gtgacccacg gcgcccacca cgccatcggc ctggccatcg
      181 ccgccatcgt ccctgccggc gccgccatcg cgctcgaggt tcccagctac cccggcatct
      241 tggatctcat cgaccaccac cgcgcccgcg cggtgggcat cccagacggg cccggcgggc
      301 ccgatcccga gggcttggcg cgcgtgctgg ccacggagcg ccccgcggcg gtgtacctcc
      361 agaccggcgt gcacaacccc accgggcacg tgctgtcggc ggcgcggcgg cgcgccatcg
      421 ccgacgtgct cgacgcgcac ggtgacgcgg tggtcatcga ggacaacacg ctggccgacc
      481 tcgtctacac cggacggccc cgtccctcga tggccgaggc ctgccgcatc gcgccggtgg
      541 tcaccgtgga gtccctgtcg aagacggcgt gggccgcgct gcggatcggc tggctgcgcg
      601 ccacgggcgt cgtcagcgag cgcatcggcg gggtccgcct ggccaccgac ctcggcccat
      661 cggtcccctc gcagctcctc gccctgcagc tgctccccca cctgccccag atggccgagg
      721 accggcgccg cgagctggag gcggccaccg tccacgccgc ggcccggatc cgcgaggagc
      781 tcccggcctg gtcgctcgag ccccccgccg gcagctcggc cctctggccc cgcctgccgg
      841 tgccggacgc cacgccgttc gtcgccctcg cccggcgcca cggcgtgcac gtcacccccg
      901 gttcggtcca cgtgctgtcg gacgcgcccg acccgcacct gcggttctgc gtcgaccggc
      961 cgcgaggcca cgtcgacgag ggcctcgacc gcctcgtcgc cgcctggcac gacgccagca
     1021 gccgatcgag ccgagccctg gcctgacttc gtcccacccc ggtcgatcgt ccacctcccc
     1081 cgaggactgg tcgatcccgc accccggcgg tggatgacgg gccagcatcc ggtgctgtgc
     1141 gggtgatcga ccgggctggt cggccatgat cggacccgtg ctgcccgccg ccatccccgt
     1201 cgacgacccc gccgatcccc ggatcgagga gttcctcggc ctgcgggacc acgagctgcg
     1261 ccgtcgccgc gaatcgcccg gcggagacct cgccgggatc ttcatcgccg agggcgacct
     1321 cgtggtcgag cgggccgtgc gggccggcta ccggctccgg tccgtgctgg tcgacgccac
     1381 ccgatcccag ccgctgcccg tcgccgtcgg ccccgacgtc ccggtcttcg ccgccggcga
     1441 ggaggtggtg ctgcgcatca ccgggatggc catgcaccgc ggcctgatcg cctcgctcga
     1501 ccggggcccc gaccgcgatc tcgccgaggt cctggccgac gccacccggg ccgtcgtcct
     1561 cgagaacgtg aacaacccga ccaacctcgg ggtcatcgcc cgcagcgcga tgggcctcgg
     1621 cgccgatgcc ctcctgctgg acccgtcgtg ctgcgacccg ctctaccgcc gggcctcccg
     1681 ggtggcgatg ggcgaggtgt tcgccctgcc gtgggcccgg acgaagcggt tccccgaggg
     1741 catcgaccag gtgaccgacg ccggcttctc gctgctggcc ctcacccccg atcccgccgc
     1801 cgagcccatc gacgacgtcg tgctcgcccc cgacgagaag gcggccttgg tgctgggcgc
     1861 cgaaggcccc ggcctcacca ccgagaccat ggcccgcgtc ggccgacggg tccgcatccc
     1921 cctccaccgc ggggtcgact cgctcaacgt cggcgccgcc gccgccatcg cctgctacgt
     1981 cctcggccgc ggccgcccct gaccgacccg accacccgat ttcgggtcac ctgggcaccc
     2041 cacaggtgca caggtgaccc gaaatcgcgg ctcgttcccg tcgtccggct cgaatcgaaa
     2101 gttcgggggc gggcggccgt aggctcacgg cgtgagcgac ggggcgggca tcgacgcgac
     2161 aggcggggac gccgatgccc acgtcgtctt gaccgcgctg cggaccaccg gggcgaaggg
     2221 gctgtcggtc accatctcgg tggtccaccg gaccgtcgag ctgctctgcg tcgccgccgg
     2281 cgcgaccggc ctggtggccc tcgtcctcgg ggtgtgggcg tggcgcaaca cgttgccggg
     2341 cgcggggatc gcgttcgtgt gcggcggcgt gaccctcgcg gtggcggtct acgtcctgat
     2401 ccgggtcggg gccctggccg ccgcgatcaa gcacccggcc gagacgctcg cccaggccca
     2461 ggacctcgtg ctccgggcga aggggagccc cgagctgcac cagctcgccg gtgccgtgcg
     2521 cacccgccag agccgcaagg cggccaaggc ggccggagtg ggtcgggccc gccgggcgct
     2581 gaacagcggc cggctgatca gccgcgtcat cggcctggcc tcaccggacc ccgagcgcca
     2641 ccgctacctg gtgccgttca ccccggaccg gctcaaggac ctgtggctcg ccatcctcgt
     2701 gggtctctgg gcctggctgg tctccgcggc ggtcgcgtcc ctggcgttcc tcagcctggc
     2761 gatccaggcg ctctgaccgc cgacggccgg accgaacccc ggaccggaag cggccgatcc
     2821 tcgacgaccg gcacgacggc caccgcgggc gagtcgaccc gagccggatg ccgcatccga
     2881 gagacggtca gctgcggcag ccagtcgtgc gacgttcggg ccggaccggc ccaccggcgg
     2941 tcaggagagg tcggcgtcgg tgatgccggc gagggggccg tcgccgcgca ggacgtgtgc
     3001 accgtcgagc gaccggacca cgacggcgcg cccggcgaag tcccggccgt cgagggtggt
     3061 gatgtcgacg gcgtaggtgc tctgtgggat cgacgtggcg tcgagcgagt acgcgaggca
     3121 ctcccactgg agctcggcga ggccctcctg ctcggccacc agcaggtagg cggccttcaa
     3181 cacgacgggg atgccctcca tcgtgagggt ggcgacatcg aagcggacgt ggcgcatgac
     3241 ctccatcatc gcctggaacg gcttcggggg ctgccggggg tgctcggtag gctcaccggt
     3301 cgtgagcagc gacgacacga ccccgtaccc gaaggttccg agccgcgcgg actaccccgc
     3361 gatcgagcgt cggatcctcg agttctgggc cgccgacggc acgttccagg cgtccatcga
     3421 ccaacgtccc gccgacgacg agtacgtctt ctacgacggt cccccgttcg ccaacgggct
     3481 cccccactac gggcacctgc tgaccgggta cgtgaaagac gtcgtccccc gctaccagac
     3541 gatgcgcggc caccgcgtgg cccgccgctt cggctgggac acccacggcc tccccgctga
     3601 gatggaggtc gagaaggaga tcggcatcgc cggccacaag gccatcaacg agtacggcat
     3661 cgagaagttc aacgagcact gccggacctc ggtgctgcgc tacaccgccg agtgggagcg
     3721 ctacgtcaac cgccaggccc gctgggtgga cttccaagac gactacaaga ccatggacct
     3781 cgggttcatg gagagcgtca tctgggcgtt c
//
LOCUS       DATMFS010000428         3344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_223739, whole genome shotgun sequence.
ACCESSION   DATMFS010000428 DATMFS010000000
VERSION     DATMFS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223739"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VNQ33_08110"
     CDS             <1..491
                     /locus_tag="VNQ33_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98108.1"
                     /translation="FRDEGLAQGITQTNDEDETTTLLVEDACVFLNRPGFGGAGLGCA
                     FHHLADANGQRHVDVMPEVCWQVPLRRVDETDDLGHVTSTVREWKRRDWGDGGHDFHW
                     WCTDGDEAFGGSVPVYEGMGAELAEMVGEEIYGLIADHLRDRTRRQGTEVFLPHPAIR
                     RR"
     gene            660..1286
                     /locus_tag="VNQ33_08115"
     CDS             660..1286
                     /locus_tag="VNQ33_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98109.1"
                     /translation="MPTAEPHACPRPHRLRVAALAAVAGLALALAGCGSSGGDETSST
                     TKAPAETTSTSTPATETTRSVTTTGAPSTTSTTVAPKDLTPEQTAVAFVRALVEGQSA
                     AAYVRDPAVATDAERQLGEMGQAAYDSIALDPVTSQETSGAPDSCAVHDASVSCYVLI
                     DRSSSDSEGVNTLVRVDVATRDLSTVTGPDDPGPEVPAHVISIEVIPS"
     gene            1367..2008
                     /locus_tag="VNQ33_08120"
     CDS             1367..2008
                     /locus_tag="VNQ33_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HWJ98110.1"
                     /translation="MAARCVNGHDTPRGQACMYCGAPLAAPAAPPPAAYAAPPQGPPP
                     GWSQAPAPGPTSGLAIASLVLGILYPIGSIPALICGWLALDRIRTRNQSGRGLAIAGI
                     VLGSIGTLFAILAAIVVLGVFTSSGVVDKDGTTGSRQSTSRGCLVEYRTLRTATEAYN
                     AQYSTYPASLDDLYGTFLNEDARDDIRFRFVAAEGHEPPRFEALDRECEGVGD"
     gene            complement(2024..2749)
                     /locus_tag="VNQ33_08125"
     CDS             complement(2024..2749)
                     /locus_tag="VNQ33_08125"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ98111.1"
                     /translation="MVDVAFKDLCIDVTAGLGRPAAVARFWAEALGLAVSSDGGEDSK
                     LSPPGDEAVERTIWINAVPEEIAGKSRVHLDLCVAAGDPAPLIAAGASLLRPADAGRP
                     WHVLEDPDGVVLCAFAPGGDPALGPTRPGVYELVVDAADAGALAAWWAERTGAAVRHH
                     PIRPYVWLEGAAGFPYERWEFNPVPEPKAHKNRIHWDVTLRDADVDGLVAAGATLLRE
                     PAPDGRWWILADPEGNEFCAFEP"
     gene            2783..3199
                     /locus_tag="VNQ33_08130"
     CDS             2783..3199
                     /locus_tag="VNQ33_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HWJ98112.1"
                     /translation="MGGERERSGPERTAVVVGGLRMASGISFLVAPEAANRIWGGNPD
                     DVGPTASLLLRSMGYRDALVGGLLLRSGLRRDGRVAGWFLASAGADLADLAGGIANHD
                     RMSDEARRKGIGGAAVGIAVGVLGAAAARRARPATA"
     gene            complement(3189..3335)
                     /locus_tag="VNQ33_08135"
     CDS             complement(3189..3335)
                     /locus_tag="VNQ33_08135"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98113.1"
                     /translation="MKLTPPKAVTLENGIEFIAEDELVEVTPESIRIRKRILKEQDRR
                     RLKR"
BASE COUNT          452 a         1233 c         1231 g          428 t
ORIGIN      
        1 agttccgcga cgagggcctg gcgcagggga tcacccagac caacgacgag gacgagacga
       61 cgaccctgct ggtggaggat gcctgcgtgt tcctcaaccg cccggggttc ggcggcgccg
      121 gtctcggctg cgccttccac cacctggcgg acgcgaacgg gcagcgccac gtggacgtga
      181 tgcccgaggt ctgctggcag gtgccgctgc gccgggtcga cgagaccgac gacctcgggc
      241 acgtcacgtc gaccgtgcgg gagtggaagc gccgggactg gggcgatggc ggccacgact
      301 tccactggtg gtgcaccgac ggcgacgaag ccttcggcgg gtccgtcccg gtgtacgagg
      361 ggatgggcgc cgagctggcc gagatggtcg gcgaggagat ctacggactg atcgccgacc
      421 acctccgcga ccgcacacgc cggcagggca ccgaggtctt cctcccccac cccgccatcc
      481 gccgccgctg acgggaccag ccctccgccc cgacggcccg gcgcgctggc cgatcgggcg
      541 cgacgcccgc tcaccagacg ccggggcgat ggcggctgcc gagccacggg cggtgggcgg
      601 tgggcggtgg gcggtgggcg cctacggtgg acggtccgcc ccgaccgaca ggatgcaccg
      661 tgcccaccgc cgaaccccac gcctgccccc gcccgcaccg cctccgcgtc gcggcgctcg
      721 ctgccgtcgc cgggctggcc ctggccctgg ccggctgcgg atcctccggc ggggacgaga
      781 cgtcctcgac cacgaaggcc cccgccgaga ccacctcgac gtcgaccccg gccacggaga
      841 cgacgcggtc ggtgaccacc accggcgcgc cgtccaccac cagcaccacg gtcgccccga
      901 aggacctgac gccggagcag accgcggtgg ccttcgtccg ggccctggtc gaaggccagt
      961 cagcggcggc gtacgtgcgc gaccccgccg tcgccacgga cgccgaacgg cagctgggcg
     1021 agatgggcca ggcggcgtac gactcgatcg ccctcgatcc ggtcacgtcg caggagacga
     1081 gcggcgcacc ggactcgtgc gccgtgcacg acgcgtccgt ctcctgctac gtcctgatcg
     1141 accggtcgag ctcggactcc gagggcgtga acaccctggt ccgggtcgac gtcgccaccc
     1201 gtgatctgag caccgtcacc ggtcccgacg accccggtcc cgaggtcccc gcccacgtga
     1261 tcagcatcga ggtcatcccg agctgaccgg ctggtcggcc ggggcggcta cggtctcggc
     1321 agggaacggc ccgaagcgga ccgcgggtgg atcaggaggg acgccggtgg cggccaggtg
     1381 cgtcaacgga cacgacaccc cccggggcca ggcgtgcatg tactgcggtg ctcccctcgc
     1441 tgctcccgca gcaccgccgc ccgcggccta cgccgcgccg ccgcaaggtc ccccaccggg
     1501 ctggtcgcag gctccggcgc ccggtccgac gagcggcctc gccatcgcct cgctggtgct
     1561 cggcatcttg tacccgatcg gctcgatccc ggcgctcatc tgcggctggc tggcgctgga
     1621 ccggatccgg acccgcaacc agagcggccg cggcctggcc atcgccggca tcgtgctcgg
     1681 gagcatcggc accctgttcg cgatcctcgc cgccatcgtg gtgctgggcg tcttcaccag
     1741 cagcggcgtg gtcgacaagg acggcaccac cggctcccgc cagagcacct cccgcggctg
     1801 cctggtcgag taccggaccc tccggacggc gactgaggcg tacaacgcgc agtacagcac
     1861 ctacccggcg tctctcgacg acctgtacgg cacgttcctc aacgaggacg ctcgagacga
     1921 catccgcttc cgcttcgtcg ccgccgaggg ccacgagccg ccgaggttcg aggcgctcga
     1981 ccgcgagtgc gagggcgtcg gggactgacg cccggcgtcg aggctacggc tcgaaggcgc
     2041 agaactcgtt gccctcggga tcggcgagga tccaccagcg tccgtcgggg gcgggctccc
     2101 gcaggagggt ggcccctgcc gccaccaggc cgtcgacatc ggcgtctcgc agcgtgacgt
     2161 cccagtggat gcggttcttg tgggccttgg gctcgggcac cgggttgaac tcccagcgct
     2221 cgtaggggaa cccggcggca ccctccagcc agacgtacgg ccggatcggg tggtggcgca
     2281 ccgcggcgcc ggtccgctcg gcccaccagg cggcgagggc accggcatcg gcagcatcga
     2341 cgacgagctc gtacacgccc gggcgcgtcg ggcccagggc cgggtcaccg ccgggagcga
     2401 aggcgcacaa gacgacgccg tcggggtcct cgagcacgtg ccagggccgt ccggcatcgg
     2461 ccgggcggag cagcgaagcg ccggcggcga tcagcggggc tggatcaccc gccgccacgc
     2521 agaggtcgag gtgcacccgg ctcttgcccg cgatctcctc gggcacggcg ttgatccaga
     2581 tggtgcgctc gacggcctcg tcaccgggcg ggctgagctt cgagtcctct cccccatcgc
     2641 tggacacggc gaggccgagg gcctcggccc agaagcgtgc gaccgctgcg ggacgaccga
     2701 gcccggcggt cacgtcgatg cacaggtcct tgaaggcgac gtccaccacg cacccgaggc
     2761 tacggggtgg catgctgcgc cgatgggagg ggaacgggag cggagcgggc ccgagcggac
     2821 tgccgtggtg gtgggcggcc tgcggatggc gtcggggatc tcgttcctcg tggcccccga
     2881 ggcggcgaac cggatctggg gcggcaaccc cgacgacgtc gggccgacgg cctcgctgct
     2941 gctgcggtcg atgggctacc gcgacgcgct ggtgggcggc ttgctcctgc ggtccggcct
     3001 ccgccgcgac ggccgggtgg ccgggtggtt cctggccagc gccggtgccg acctggccga
     3061 cctcgcgggc gggatcgcca accacgaccg catgagcgac gaggcccgca ggaaggggat
     3121 cggcggagcc gcggtcggca tcgccgtcgg ggtgttggga gcggcggcag cccgccgggc
     3181 gaggcccgct accgcttgag gcgccggcgg tcctgctcct tgaggatccg cttgcggatg
     3241 cggatgctct cgggcgtgac ctcgaccagc tcgtcttcgg cgatgaactc gatgccgttc
     3301 tccagcgtga ccgccttcgg cggcgtgagc ttgatggctt cgtc
//
LOCUS       DATMFS010000429         5572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_109629, whole genome shotgun sequence.
ACCESSION   DATMFS010000429 DATMFS010000000
VERSION     DATMFS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109629"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..498)
                     /locus_tag="VNQ33_08140"
     CDS             complement(91..498)
                     /locus_tag="VNQ33_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWJ98114.1"
                     /translation="MAKFLSEDWVTQARALQEEYRGKGTPPAAQARINLTITEVPADV
                     SDGPIAATIDTTEGSADLDLGAFDAPDASISLEYDTAKAFLVEGNPQVVMQAVMSGKV
                     KISGDMTKVLALGQGAPDDVALEMGRKLREITD"
     gene            complement(670..1305)
                     /locus_tag="VNQ33_08145"
     CDS             complement(670..1305)
                     /locus_tag="VNQ33_08145"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWJ98115.1"
                     /translation="MTEDRRLTSQGQERKQQLLDQAAILFAERGYAETRVLDIVQAAG
                     VAKGLFYWYFENKEALFRDLVELNRLNLRRAQAEAIDLEAEPLVRIRQGAEASVRYMA
                     QYANFFSLLEVENLEKQFADELRKGTQIHTADVAAIIRAGVADGTIRDEDPEVAAYGV
                     VGTVGYYGHFHRTGRLDLDPDELAAFVGRFVVCALASDESIARRVLAARRS"
     gene            1447..2214
                     /locus_tag="VNQ33_08150"
     CDS             1447..2214
                     /locus_tag="VNQ33_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ98116.1"
                     /translation="MATDLPADLIAGAEPTFYEGGPVGVLVLHGFTGNPGSMRGLAER
                     LAAAGFTVDLPLLPGHGTVVEDMIPTGWPDWLAHAEARYAALAERCEQVVVVGLSMGG
                     GLTAWLGSEHPEIAGLVCINAVVSVPPGMREAVQEVLATGADRFAGIGSDIAQPGVTE
                     SAYADTPLAPLLTLFDASDTLGDRLSRITSPLLVVTSRQDHVVPPENSDILAGSVSGP
                     VERIWCERSYHVVTQDFDRELVFDATVGFAQKVTASA"
     gene            2344..3513
                     /locus_tag="VNQ33_08155"
     CDS             2344..3513
                     /locus_tag="VNQ33_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWJ98117.1"
                     /translation="MDHEAATEPALRRGGRCRRIAALAVAAAAVGTAALTGCTPLGKP
                     ATRQTPAAPRVFDSSDPAVLVVGSTSYLFGSSNNKKVPVRAVASFSGSLAASKAEWDG
                     MASPAAKADAMPTRPAWVNPARFNGTWQIWAPSPVKIGSTYYLYFAASRSGATELSND
                     QCIGRASSSTPAGPYTPSSAPLYCGLSKEAGSNPWGRGALDPEVFRAADGRLYLLVSL
                     SRTEDPIAVVPLTSTGVVSGGVNAKATVLVGRTRPWQDGSDDSALTRTGTLENPSMIY
                     EPKTKTYLLFYSAGDWRTAYYNTGFARCASPTGPCTAQADGPFLKSGNGRAGVGGLTA
                     FRTSDGVARVAYASWKLGSENQPSPDGSLSRQTHWSRLVVSNTADAGSQTVRLAE"
     gene            3677..4942
                     /locus_tag="VNQ33_08160"
     CDS             3677..4942
                     /locus_tag="VNQ33_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98118.1"
                     /translation="MSGRLTRVRRWLPMFVLAAAVRVVYWVAVTPDWVPVADADQYVR
                     LARALADGKGFALQFPQFEVHATAFRPPLLPALLTPGALAFGAALWPARLLVLLIGCS
                     VVVLAGVLAARVGGRWAGSAAATLVALYPPLIANDTLTLTEPLALLLLLAAVLLLDEG
                     RFVGAGITVGLLLLTRPNGYLVVAILAAWAWQRAGFKKGLVVGGCALLVLLPWLIRNQ
                     IQVGTFRPTTSDGFTIAAVWGVPAQQAETFVDPVYSDAYDDLQHRYRQFDEAEWNDQL
                     TKEGVRALRSHPRYLPTLVQRNAAGYFEFNSRYNHYPEDNDGRDWPFRQDTRAAFWVV
                     SVLGLIGLVRFRRDPRIIVLFLLTLQFVVLSLLLVAPPRLRAPFDLTMCIGAGLLVGS
                     WFEARHRRSEPALDAGAALDAGPAAASPA"
     gene            4991..5293
                     /gene="gatC"
                     /locus_tag="VNQ33_08165"
     CDS             4991..5293
                     /gene="gatC"
                     /locus_tag="VNQ33_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWJ98119.1"
                     /translation="MPAHISRDDVAHVAKLARLSLDDAELDTFTAQLGAILEHAEDVE
                     ALDLADVPPTSHPFDLVNVLRPDRPGTTLTPDAALAGAPQAEAGRFRVPTILGEAP"
     gene            5290..>5572
                     /locus_tag="VNQ33_08170"
     CDS             5290..>5572
                     /locus_tag="VNQ33_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWJ98120.1"
                     /translation="MTAVDIARGVRAGSASATSIADAHLARIDEREPDVHAFNLVTPD
                     VARAAAAEVDRKVAAGEDPGPLAGVPIAIKDNLCTDGIATTCSSKILEGW"
BASE COUNT          708 a         2023 c         1994 g          847 t
ORIGIN      
        1 agaccgagcc cctcccggcg gtctgacctc cgccggctgc gggtcgagga ccgcagcact
       61 ggcgagcggc gcagaccgtg cgccgaggaa tcagtcggtg atctcgcgga gcttgcggcc
      121 catctcgagg gcgacgtcgt cgggtgcgcc ctggccgagg gcgaggacct tggtcatgtc
      181 gccggagatc ttgaccttgc cgctcatgac ggcctgcatg acgacctgcg ggttgccctc
      241 caccaggaac gccttggccg tgtcgtactc gaggctgatc gacgcgtcgg gcgcgtcgaa
      301 cgcgccgagg tcgaggtcgg cggagccctc ggtggtgtcg atcgtggcgg cgatggggcc
      361 gtcgctgacg tcggcgggga cctcggtgat ggtgaggttg atccgggcct gcgccgcagg
      421 tggcgtgccc ttgccccggt actcctcctg gagcgctcgg gcctgggtca cccagtcttc
      481 actcaggaac ttcgccacgg tgggtctcct cgacgctggt aggcggaaat gcgcagcgga
      541 ccctactggt tggccggacg gggtgcggcc gatcgccgcg ctgctcggtg tgacggtttc
      601 cgccccgaac tccggaggcc accgcacccc gagggtgcgc ttctccggcg ttcggcgtgg
      661 gcgggacggt caggagcggc gggcggcgag gacgcggcgg gcgatggact cgtccgaggc
      721 gagggcgcac acgacgaagc ggcccacgaa ggcggcgagc tcgtcggggt cgaggtccag
      781 ccggccggtg cggtggaagt ggccgtagta gccgacggtg ccgaccacgc cgtaggccgc
      841 cacctcgggg tcctcgtcgc ggatcgtccc gtcggcgacg ccggcgcgga tgatggcggc
      901 cacgtcggcg gtgtggatct gcgtgccctt gcggagctcg tcggcgaact gcttctcgag
      961 gttctcgacc tcgaggagcg agaagaagtt ggcgtactgg gccatgtacc gcaccgaggc
     1021 ctcggccccc tggcggatgc ggaccagcgg ctcggcctcg aggtcgatgg cctccgcctg
     1081 ggctcggcgg aggttgagcc ggttgagctc gacgaggtcg cgaaacagcg cctccttgtt
     1141 ctcgaagtac cagtagaaga ggcccttggc cacgccggcg gcctggacga tgtcgagcac
     1201 gcgggtctcg gcgtagcccc gctcggcgaa gaggatggcg gcctggtcga ggagctgctg
     1261 cttgcgctct tggccctggc tggtgagccg gcggtcttcg gtcatggtgg cggcaagtgg
     1321 accaccgcat tgaccgccgg gtcaaggtgc aatcgccaga tgtgcgacgc ggcgaggccg
     1381 cgggtgtggt tgcctgtcgg gcgagcgctg cggtccgagc cgccgcgcac ccgaacgagg
     1441 agcaccatgg ccaccgacct tcccgccgat ctcatcgccg gcgctgagcc gaccttctac
     1501 gagggcggcc ccgtcggcgt gctcgtgctg cacggcttca cgggcaaccc cggttcgatg
     1561 cggggcctcg ccgagcgcct cgccgccgcc ggcttcaccg tcgacctgcc gctgctgccg
     1621 gggcacggca ccgtggtgga ggacatgatc cccacggggt ggccggactg gctggcccac
     1681 gctgaagctc gctacgcggc cctcgccgag cggtgcgagc aggtcgtggt ggtgggcctg
     1741 tcgatgggcg gcgggctgac ggcgtggctc ggctccgaac accccgagat cgccgggctg
     1801 gtgtgcatca acgccgtcgt gtcggtgccg cctggcatgc gggaggccgt gcaggaggtg
     1861 ctcgccaccg gagccgaccg cttcgccggc atcgggtccg acatcgccca gccgggcgtg
     1921 accgagagcg cctacgccga tacgccgctc gccccgctgc tcacgctctt cgacgcgtcc
     1981 gacaccctcg gtgaccgcct cagccggatc acctcgccgc tgctcgtcgt caccagccgc
     2041 caggaccacg tggtcccgcc ggagaactcg gacatcctcg ccggttccgt gtcaggaccg
     2101 gtcgagcgga tctggtgcga gcgcagctac cacgtggtca cccaggactt cgaccgcgag
     2161 ctcgtgttcg acgcgacggt cggcttcgcc cagaaggtga ccgccagcgc ttgaagttcg
     2221 gccccggccg accgatgggt gaggatcgac ccgcgcgatc agtggccgga cgtggcgcgg
     2281 gcacatccag ggcgggcgac acgatggcac tcgccatcgc aggaccgatc cgaggagccc
     2341 ccggtggacc acgaagcagc aaccgagccc gctctgcggc gaggcggccg ttgccggcgc
     2401 atcgccgcgc tcgccgtcgc ggcggccgcc gtcggcaccg cggccctcac cgggtgcacg
     2461 cccctgggga agccggcgac ccgccagacc cccgccgctc cccgcgtgtt cgacagctcg
     2521 gatccggcgg tcctcgtcgt cgggtcgacc tcgtacctgt tcgggtcgag caacaacaag
     2581 aaggtgccgg tccgggcggt ggcctccttc tccggcagcc tggccgccag caaggccgag
     2641 tgggatggca tggcgtcgcc ggccgccaag gccgatgcca tgcccacccg gccagcctgg
     2701 gtgaaccccg cccggttcaa cggcacctgg cagatctggg cgccttcgcc cgtcaagatc
     2761 ggctcgacct actacctgta cttcgccgcc agccggtcgg gtgccaccga gctgagcaac
     2821 gaccagtgca tcggccgggc gtcgtcgtcc acgcccgccg gcccgtacac gccgtcgtcg
     2881 gcaccgctct actgcgggct gtcgaaggag gccggctcca acccgtgggg ccgtggcgcc
     2941 ctggacccgg aggtgttccg ggcggcagac gggaggctgt acctgctcgt gtcgctcagc
     3001 cggaccgagg acccgatcgc cgtggtcccg ctgacctcga ccggcgtcgt gtcgggcggc
     3061 gtcaacgcca aggccacggt gctcgtcggc cgcacccggc cctggcagga cggctcggac
     3121 gactcggcgc tgacccggac gggcacgctc gagaacccgt cgatgatcta tgagccgaag
     3181 accaagacct acctgctctt ctactcggca ggagactggc gcacggcgta ctacaacacc
     3241 gggttcgccc ggtgcgccag ccccaccggt ccgtgcaccg cgcaggctga cggcccgttc
     3301 ctgaagtcgg gcaacggccg cgccggcgtg ggcggcctga cggcgttccg gacgtcggat
     3361 ggcgtggcgc gcgtggcgta cgcgtcgtgg aagctgggct cggagaacca gccgagccct
     3421 gacgggagcc tcagccgcca gacccactgg tcgcgcctgg tggtctcgaa caccgccgac
     3481 gctggctccc agaccgttcg tctcgccgag taggcgccct cgccggctcg ccatcgacac
     3541 ccacaccgtt cggagcatcg gcacccggcg gccctggtgg ccctggctgc cctggtgggc
     3601 cgactggccc gtgcggggga tgcggctgcg ggagcggccg cgctcctagg gtgagcgcca
     3661 tggagaccgg ggctgcgtga gcggccggtt gacacgggtc cgccgatggc tgccgatgtt
     3721 cgtgctggcc gcggcggtgc gcgtcgtcta ctgggtggcg gtcacccccg actgggttcc
     3781 cgtcgccgac gccgaccagt acgtgcggct cgcccgggcg cttgccgacg gcaagggctt
     3841 cgccctccag ttcccccagt tcgaggtcca cgccaccgcg ttccgcccgc cgctcctccc
     3901 ggccctgctg accccggggg ccctcgcctt cggcgccgcc ctctggccgg ctcggctcct
     3961 cgtgctcctg atcgggtgct cggtcgtggt gctcgccggc gtgctggcgg cccgcgtcgg
     4021 tggccggtgg gcaggctctg cagcagcgac cctggtcgcg ctctacccgc cgctgatcgc
     4081 caacgacacc ctcaccctca ccgagccgct cgccctgctc ctcttgctcg ccgccgtcct
     4141 gctcctggac gaagggcgct tcgtcggagc cgggatcacc gtcgggctcc tcctgctcac
     4201 ccgcccgaac gggtacctcg tggtggcgat cctggcggcg tgggcgtggc agcgcgccgg
     4261 gttcaagaag gggctcgtgg tgggtggctg cgccttgttg gtcctgctgc cgtggctcat
     4321 ccgcaaccag atccaggtcg ggacgttccg gcccaccacg tctgacgggt tcaccatcgc
     4381 cgcggtctgg ggcgtgccgg cccagcaggc cgagacgttc gtcgatcccg tgtactcgga
     4441 cgcctacgac gatctgcagc accggtaccg gcagttcgac gaggcggagt ggaacgacca
     4501 gttgaccaag gagggggtgc gagcgctgcg cagccacccc cgctacctcc cgaccctcgt
     4561 gcagcggaac gcggccggct acttcgagtt caactcgagg tacaaccact acccggagga
     4621 caacgacggc cgtgactggc ccttccgcca ggacacccgg gcggcgttct gggtggtgtc
     4681 ggtgctgggc ctcatcgggc tcgtgcgctt ccggcgcgac ccgaggatca tcgtgctgtt
     4741 cctgctcacc ctccagttcg tggtgctctc gctcctcctg gtggcaccgc cgcgcctccg
     4801 ggctcccttc gacctgacga tgtgcatcgg ggcgggtctg ctggtcggtt cctggttcga
     4861 ggcgcggcac cggaggtccg aaccggcgct cgacgcgggt gccgcgctgg acgcgggtcc
     4921 ggccgctgcg agtcctgcgt agggcaaggc ctttcgggtc ggtggaggcg gcaccgtagg
     4981 ctcggcctcc atgcctgccc acatctcccg ggacgacgtc gcccacgtcg ccaagctggc
     5041 ccggctgtcg ctcgacgacg ccgagctgga cacgttcacc gcccagttgg gcgccatcct
     5101 cgagcacgcc gaggacgtcg aagccctcga cctggcggac gtgccgccga cctcgcaccc
     5161 gttcgacctg gtcaacgtgc tgcgccccga ccggcccggc accacgctca cgcccgatgc
     5221 cgccctggcc ggtgcgcccc aggcggaggc cggccgcttc cgcgtcccga cgatcctggg
     5281 agaggcgccg tgaccgctgt cgacatcgcc cgcggcgtcc gcgccggatc ggcgtcggcc
     5341 acgtcgatcg ccgacgccca cctcgcccgg atcgacgagc gcgagccgga cgtccacgcg
     5401 ttcaacctgg tgacgcccga tgtcgcccgg gcggccgccg ccgaggtcga ccgcaaggtg
     5461 gctgctggcg aggaccccgg tccgctggcc ggcgtgccca tcgccatcaa ggacaacctc
     5521 tgcaccgacg gcatcgccac cacctgctcg tcgaagatcc tcgagggctg gg
//
LOCUS       DATMFS010000430         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_223817, whole genome shotgun sequence.
ACCESSION   DATMFS010000430 DATMFS010000000
VERSION     DATMFS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223817"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VNQ33_08175"
     CDS             complement(<1..532)
                     /locus_tag="VNQ33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ98121.1"
                     /translation="MEAPTTSRPRASSPVAVSELMSSPVVTAWPGQTLAAASDTMVLA
                     GVGSVVVVGDPGLPIGILTERDLVRASAAGVDARMATVSEWMTAAPEVVEPSVSIDTA
                     LDTLARRGYRHMPVVDGTDLVGIVSMRDLMKVASIRPAGEAAVDTPRGLKGVVVTDTA
                     IGDVRGAEGFFHYRQYS"
     gene            610..1830
                     /locus_tag="VNQ33_08180"
     CDS             610..1830
                     /locus_tag="VNQ33_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWJ98122.1"
                     /translation="MDAPRLTTAEAAARLGVKPETLYAYVSRGVIERHRDPGGVSTYD
                     PRDIERLARAGRRTKSLPPVVFPSSLTLIDEGRYSYRGTDAVAAAVGHRFEEVAEWLW
                     TGAWPDDARWPFDTTALDQVLAAQAPLPEHCLPLDRYRVITAVAAAADPVRHDGRPEA
                     VVPTARRLLRLLVHGLPRADGREARRAEPTRTMAEVLWTRLTRLPRTPERIALLDAAL
                     GLLADHELASSTFAARMAAMVRADVYEVVGAGLNVIGGLRHGGASLALEAVFRDAARF
                     GVPRALDSRVGDGGRLAGFGHSLYPAGDPRAIAVFERLPAIDPPGDRLATVEAVLAEL
                     DRRGVAPPNVDAGLAALSFCADMPPGSGEAVFALARTAGWIGHALEQYDQPTFIRARV
                     DYVGPRAPGAAPTR"
     gene            1873..>2618
                     /locus_tag="VNQ33_08185"
     CDS             1873..>2618
                     /locus_tag="VNQ33_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ98123.1"
                     /translation="MANEQMRENWTLGAKGWVQHRDLFDGELEPFAVAVIEAAAPGPG
                     DRVLDVGCGTGTLVARSVARGAEAIGVDLSPGMVEGAQQFHPEGTFLVADAQTDDLAG
                     HGPFTTIVSRFGVMFFDDPTAAFANIRRAAAPGARLVFACWRSHEENAMFTLGTSTLL
                     ARLDPPLPVLDPAAPGPTALADPDRTRTILADAGWTDVGLVPCDAVLDYARNGGDGVE
                     ERLTMVLNTNAGRMAQTRLEPQLGPDGWAAL"
BASE COUNT          336 a         1049 c          889 g          344 t
ORIGIN      
        1 ccgagtactg gcggtagtgg aagaagccct cggcgccccg cacgtcgccg atcgccgtgt
       61 cggtgaccac cacgcccttc agcccccgag gcgtgtccac cgcggcctcc cctgccgggc
      121 ggatcgatgc caccttcatc aggtcgcgca tcgacacgat gcccaccagg tcggtgccgt
      181 cgaccaccgg catgtggcgg tagccccggc gcgccagggt gtcgagggcg gtgtcgatgg
      241 agacggacgg ctccaccacc tcgggagcgg ccgtcatcca ctcgctgacg gtcgccatcc
      301 gggcgtcgac gccggccgcc gaggctcgga ccagatcccg ctcggtgagg atgccgatgg
      361 gcaggccggg atcgccgacc acgacgaccg agccgacgcc ggccaggacc atggtgtcgg
      421 aggcggccgc cagggtctga ccgggccagg cggtgacgac gggcgagctc atgagctcgg
      481 agacagcgac cgggctgctg gcccggggtc gggaggtggt gggtgcttcc atgggctcga
      541 ccttgacagc atcgttgaca ttgatcaatc ttgaaccatg atcaatgtcg atcgatcccg
      601 gtcggatgca tggacgcacc ccggctgacc accgccgaag ccgcggcccg gctcggcgtg
      661 aagcccgaga ccctctacgc ctacgtcagc cgcggggtga tcgagcgcca ccgcgacccc
      721 ggcggcgtga gcacctacga cccccgggac atcgagcgcc tcgcccgcgc cgggcgccgg
      781 accaagagcc tgccgccggt cgtgttcccg tcgtcgctca ccctcatcga cgagggccgc
      841 tactcctacc gcggcaccga cgcggtcgcc gccgcggtcg gacaccggtt cgaggaggtg
      901 gccgagtggc tctggacggg cgcctggccc gacgacgccc gctggccgtt cgacacgacg
      961 gcgctcgacc aggtgctcgc cgcccaggcg cccctcccgg agcactgcct cccgctcgac
     1021 cgctaccggg tgatcacggc cgtcgcggcc gccgccgacc ccgtccgcca cgacggccgc
     1081 cccgaagccg tggtccccac cgcccggcgc ctcctccggc tgctcgtcca cggccttccc
     1141 cgtgccgacg gccgggaggc ccgccgggcc gagccgaccc gcaccatggc cgaggtgctg
     1201 tggacccggc tcacccgcct cccccgcacg cccgagcgca tcgccctcct cgacgctgcg
     1261 ctgggcctcc tcgccgacca cgagctggcc agctccacgt tcgccgcccg catggccgcc
     1321 atggtgcgag ccgacgtgta cgaggtcgtc ggcgccggcc tcaacgtcat cggcgggctc
     1381 cgccacggcg gtgcctcgct cgccctcgaa gcggtcttcc gagatgccgc ccgcttcgga
     1441 gtcccccgtg cgctcgacag ccgcgtcggc gacggcggcc gcctcgccgg cttcgggcac
     1501 tcgctctacc ccgccggtga cccgcgggcg atcgccgtgt tcgagcggct gcccgccatc
     1561 gacccgcctg gagaccgcct ggccaccgtc gaggcggtcc tcgccgagct cgaccggcga
     1621 ggtgtcgccc cgcccaacgt cgacgccggc ttggccgctc tctcgttctg cgccgacatg
     1681 ccaccggggt cgggcgaggc cgtcttcgcc ctggcccgca cggccgggtg gatcggccac
     1741 gccctcgagc agtacgacca gcccaccttc atccgcgccc gggtggacta cgtcggaccc
     1801 cgggctcccg gagccgcgcc cacccgctga ccgcgcctcg tcgggcggcc gggagacggt
     1861 accgtcgccg ccatggcgaa cgagcagatg cgcgagaact ggacgctcgg agcgaagggc
     1921 tgggtccagc accgcgacct gttcgacggc gagctcgagc ccttcgccgt cgcggtcatc
     1981 gaggcggccg cgcccggccc cggcgaccgg gtcctcgacg tcggctgcgg cacgggcacg
     2041 ctcgtcgccc ggtccgtcgc ccgaggtgcc gaggcgatcg gcgtcgacct ctcgccgggg
     2101 atggtcgaag gagcgcagca gttccatccc gagggcacct tcctcgtggc cgacgcccag
     2161 accgacgacc tggccgggca cgggccgttc accacgatcg tgtcccgctt cggcgtcatg
     2221 ttcttcgatg atccgacagc agccttcgcc aacatccgcc gcgccgccgc cccgggtgcc
     2281 cgactggtgt tcgcgtgctg gcgcagccac gaggagaacg ccatgttcac cctcgggacg
     2341 agcacgctcc tcgcccggct cgacccgccg ctgcccgtct tggacccggc cgcaccgggt
     2401 cccaccgcgc tggctgaccc cgaccggact cggacgatcc tcgccgatgc cgggtggacc
     2461 gacgtcggcc tcgtcccctg cgacgccgtc ctcgactacg cccgcaacgg cggcgacggt
     2521 gtcgaggagc gcctcaccat ggtcctcaac acgaacgccg ggcggatggc ccagacccgc
     2581 ctcgagccgc agctggggcc cgacgggtgg gccgcgct
//
LOCUS       DATMFS010000431         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_294598, whole genome shotgun sequence.
ACCESSION   DATMFS010000431 DATMFS010000000
VERSION     DATMFS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294598"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1632)
                     /gene="nhaC"
                     /locus_tag="VNQ33_08190"
     CDS             complement(70..1632)
                     /gene="nhaC"
                     /locus_tag="VNQ33_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HWJ98124.1"
                     /translation="MRPAGPAPDEDDDQDGASRTIREPSLLDALIPLGALVVLIGGSL
                     LLFGLDALDGPIQVALILCCMVAALVAMKNGYRWADVEVSSQAAMSSITSAVFILLAV
                     GALIGTWNLSGTIPTLVYYGIQVLSPSWYYLATALICGAIALSIGSSWTTAGTIGVGL
                     VGIATMLGVSPEITAGAVISGAYLGDKTSPLSETTILTAQMVKADLYDHIRSQVWTSI
                     PAFAIACTGFLLLGLFGPAVDDTVRTTIELNELDRIYRITPLNLLPLALLVYLSIKKV
                     PAPLALLGSALFAGVTGAILQPDVMADFVHGIGSTHGPVIGSIQAVWQAMATGFSIDS
                     GIPDIDALLSRGGMAGMLPTLWIIIGAVTFGTLLEELGLLDRLIEPLFDAAKTTGRLF
                     LSVFACAFGLNLVAGDQYIALVLPTRLFRSEFTKRGIGPTSLSRLAADSGTVTSPLVP
                     WNSCGAFMGAVLGVSTLSYLPYALFCIASPVLSVIYGFTGFKVARAGPPAAADPPAGP
                     DSPSTASNGGEP"
     gene            complement(1636..2697)
                     /gene="glsA"
                     /locus_tag="VNQ33_08195"
     CDS             complement(1636..2697)
                     /gene="glsA"
                     /locus_tag="VNQ33_08195"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HWJ98125.1"
                     /translation="MHEPLPFDESQTPVSTGELPSLLEVEQFLHEAYGHYRSDHDGAV
                     ADYIPALAEVDPDLFGACIAGVGGNLAEVGDALHPFSLQSISKAFVFALVCDALGHAE
                     VLDKIGVNGTGLPFNSVMAIELNRDRTMNPMVNAGAMATTSLVPGDTAEDKWEFIRTG
                     LSRFAGRDLLIDEEVYGSETKTNLRNQGIAHLLDGYGRMYFDPDAATDVYTRQCSLLV
                     SAHDLAVMGATLADGGVNPITGERAVQARTCKRVLAVLATAGLYERSGDWLYEIGIPG
                     KSGVSGGIVAVAPGKGAIGVFSPKLDAAGNSVRGQRLVKNLSQRLGLNLFASAPVGWT
                     GTLRAHTAHAFSAPTPGSD"
     gene            2778..>3190
                     /locus_tag="VNQ33_08200"
     CDS             2778..>3190
                     /locus_tag="VNQ33_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HWJ98126.1"
                     /translation="MRGSAAVGAVVAAALLLGACGSSDAKGGDATTTTKARATTTDPK
                     ATTTTSGDVDDLHGAFVSSEVTGYDLAPDSELTFTFDGGTLSVNAGCNTMSSGFSIDD
                     RVLAWTGAPAATMMACDEAHTAQDAWITGLLTKGMA"
BASE COUNT          506 a         1016 c         1215 g          453 t
ORIGIN      
        1 tggccgtgag cgtggggagc gtgaccttgt cgtcctcgtg cgccgtctcg ggtcggacat
       61 gggtgtcggt catggttcgc ctccgttgga agccgtcgag ggtgagtctg gtcccgcggg
      121 cgggtcggcc gcggccggag ggccggcgcg ggccaccttg aagcccgtga agccgtagat
      181 cacgctgagc accgggctgg cgatgcagaa gagcgcgtag ggcaggtacg acagcgtcga
      241 gaccccgagc accgcgccca tgaaggcgcc gcacgagttc cacggcacca ggggcgacgt
      301 gaccgtcccg ctgtcggcgg cgagtcgaga caggctcgtg ggcccgatgc cccgcttggt
      361 gaactccgag cggaacaggc gggtcggcag caccagggcg atgtactggt cgcccgccac
      421 caggttgaga ccgaaggcgc aggcgaagac ggacaggaac aggcggccgg tcgtcttggc
      481 tgcgtcgaag agcggttcga tcagccggtc gagcaggccg agctcctcga gcagggtccc
      541 gaacgtgacg gcgccgatga tgatccagag cgtggggagc atcccggcca tgccgccgcg
      601 ggagagcagg gcgtcgatgt cggggatgcc gctgtcgatc gagaagccgg tggccatcgc
      661 ctgccacacg gcctggatcg acccgatcac cgggccgtgg gtcgaaccga tgccgtggac
      721 gaagtcggcc atcacgtccg gctggaggat cgcgccggtc acgccggcga acagcgccga
      781 gccgagcagg gcgagcggtg ccgggacctt cttgatcgac aggtagacga gcagggcgag
      841 gggcagcagg ttcagcggcg tgatgcggta gatccggtcg agctcgttca gctcgatggt
      901 ggtgcggacg gtgtcgtcga cggccgggcc gaacaggccc aggagcaaga agccggtgca
      961 ggcgatggcg aacgccggga tcgacgtcca gacctgggag cggatgtggt cgtagaggtc
     1021 ggccttcacc atctgggcgg tgaggatcgt cgtctccgac agcggcgagg tcttgtcgcc
     1081 gaggtaggcg ccggagatca cggcgcccgc cgtgatctcc ggcgagaccc cgagcatcgt
     1141 ggcgatgccg accagcccca ccccgatcgt tcccgccgtg gtccacgagc tgccgatgga
     1201 caaggcgatg gcaccgcaga tgagcgcggt ggccaggtag taccagctcg gcgagagcac
     1261 ctggatgccg tagtacacga gcgtcgggat cgtgccggac aggttccacg tcccgatgag
     1321 cgcgccgacg gcgaggagga tgaacaccgc gctggtgatc gaggacatgg cggcctggct
     1381 gctgacctcg acgtcggccc accggtagcc gttcttcatc gccaccaggg cggcgaccat
     1441 gcagcagagg atcagcgcga cctggatcgg cccgtcgagg gcatcgagcc cgaagagcag
     1501 gagcgatccg ccgatcaaga cgacgagcgc accgaggggg atgagcgcgt cgaggagcga
     1561 cggctcgcgg atcgtccggc tcgcgccgtc ttggtcgtcg tcctcgtcag gtgcgggtcc
     1621 cgccggccgc atcgatcagt cgcttccggg tgtcggagcg ctgaaggcgt gggccgtgtg
     1681 ggcccggagg gtgccggtcc atccgaccgg cgctgaggcg aagaggttga ggccgagccg
     1741 ctgcgacagg ttcttcacga gccgctggcc tcggacgctg ttgccggcgg cgtcgagctt
     1801 gggggagaac acgccgatgg cgcccttgcc cggagcgacg gccacgatcc cgccgctgac
     1861 cccgctcttg cccgggatgc cgatctcgta gagccagtcg cccgagcgct cgtagaggcc
     1921 ggcggtggcc aagacggcga ggacccgctt gcaggtgcgg gcctgcacgg cgcgctcgcc
     1981 ggtgatcggg ttgacgccgc cgtcggcgag cgtcgcaccc atcacggcca ggtcgtgggc
     2041 ggagacgagc agcgagcact ggcgcgtgta gacgtcggtg gcggcatcgg ggtcgaagta
     2101 catccggccg tagccgtcga gcaggtgggc gatgccctgg ttgcggaggt tcgtcttggt
     2161 ctcggagccg tagacctcct cgtcgatcag caggtcccgg ccggcgaagc gcgacaggcc
     2221 ggtgcggatg aactcccact tgtcctcggc ggtgtcgccg ggcacgaggc tcgtggtcgc
     2281 catggcaccg gcgttcacca tcgggttcat cgtccggtcg cggttgagct cgatggccat
     2341 caccgagttg aacgggaggc cggtgccgtt gacgccgatc ttgtcgagca cctcggcgtg
     2401 gccgagggca tcgcacacga gcgcgaacac gaaggccttc gagatgctct ggagggagaa
     2461 cgggtggagg gcgtcgccga cctcggcgag gttgccgccg acgccggcga tgcacgcgcc
     2521 gaacaggtcc gggtcgacct cggcgagcgc cgggatgtag tccgcgaccg cgccgtcgtg
     2581 atcggagcgg tagtgcccgt aggcctcgtg caggaactgc tccacctcga gcaggctcgg
     2641 cagctcgccg gtcgagaccg gcgtctgcga ctcgtcgaac ggcagcggtt cgtgcacacc
     2701 cgatttctag tccttggagg cgtagggtca cccggatgag gcagaaccag gttgggggaa
     2761 caggggcggt tcggcgggtg cgtggcagcg ccgcggtggg tgcggtggtc gcggcggcgc
     2821 tgctgctggg cgcgtgcggc agcagcgacg ccaagggcgg cgacgccacg accaccacca
     2881 aggcgagggc cacgaccacc gatccgaagg cgaccacgac gacgtcgggc gacgtggacg
     2941 acctccacgg cgccttcgtc tcctccgagg tgacgggata cgacctggcc cccgactcgg
     3001 agctgacgtt caccttcgac ggcggcacct tgtcggtcaa cgccggctgc aacaccatga
     3061 gctcggggtt ctcgatcgac gaccgcgtcc tggcgtggac cggcgccccg gccgccacga
     3121 tgatggcctg cgacgaggcc cacacggccc aggacgcgtg gatcaccggc ctgctcacca
     3181 agggcatggc
//
LOCUS       DATMFS010000432         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_341153, whole genome shotgun sequence.
ACCESSION   DATMFS010000432 DATMFS010000000
VERSION     DATMFS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341153"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VNQ33_08205"
     CDS             <1..423
                     /locus_tag="VNQ33_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98127.1"
                     /translation="VSPPLDTLLRAVVDGATRPELPGVEVVVRPALTCSPVDVLEADG
                     YVLGTPANLGSMSGALKHFFDTIYYPCLDATPRRPWGLYVHGNNDVDGAVRDIERIVT
                     GLAWRSVAAPLRVVGAPQQADVDGCWELGATVAASLAD"
     gene            complement(549..1352)
                     /locus_tag="VNQ33_08210"
     CDS             complement(549..1352)
                     /locus_tag="VNQ33_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98128.1"
                     /translation="MSARTSSRRLAALTAALLLAVGVTHAAPAPAQAADPVVTVTAAN
                     GSVFSADDCKGQTQTNITEPIRVTLTRSGDASASLDVALSYGGSLASTSGLPVQATIA
                     AGEAQVVLEASPETGGDLTVDLTAGAGYTIGSPGSASTSMSQGIADLGCNIGDVSTTQ
                     TIERGTTPAAIDVEEVAYSPPDGLVRSVEGTVPPGTAFHLDGTWDGVATELGTFRFSE
                     FFCEDDGWCPYRADIEVIVVESGAIPPVTGTRPPAAPARAVPGTPQLTG"
     gene            1452..1922
                     /locus_tag="VNQ33_08215"
     CDS             1452..1922
                     /locus_tag="VNQ33_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWJ98129.1"
                     /translation="MASELDALLDHAAIDRLQRAYADGVTRRDWEQVRGLFVADAAIS
                     LDLVSRPAVQLAGPVELTDFVSAALAPFSFFQFQIVNSHIEASTVDPAAAKARMFMCE
                     LRLKHGASQRDDTYGLYRDTYRKTEEEGYGGWRFASRWYRTVARFPDNEVFPLD"
     gene            complement(2048..>2261)
                     /locus_tag="VNQ33_08220"
     CDS             complement(2048..>2261)
                     /locus_tag="VNQ33_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ98130.1"
                     /translation="EGYVVNLWIAPSARRQGIARALLQTAIDDAASWGLRRLYLFATD
                     DGRPLYEDSGFAPDPRWMARRIDATA"
BASE COUNT          287 a          784 c          859 g          331 t
ORIGIN      
        1 gtgtcgccac cgctcgacac gctgctgcgg gccgtcgtcg acggggccac ccggcccgag
       61 ctgcccggtg tcgaggtggt ggtgcggccg gcgctgacgt gctcgccggt cgacgtgttg
      121 gaggccgatg gctacgtgct cggcacaccg gcgaacctcg ggtcgatgag cggcgcgctc
      181 aagcacttct tcgacacgat ctactacccg tgcctcgacg ccacgccgcg gcggccctgg
      241 ggtctgtacg tgcacggcaa caacgacgtc gacggcgccg tgcgcgacat cgagcggatc
      301 gtgaccgggc tggcctggcg gtcggtcgcc gcgccgctcc gggtggtcgg cgcgccgcag
      361 caggcggacg tcgatgggtg ctgggagctg ggcgccaccg tggcggcgtc gctcgctgac
      421 tgagcgcggc ggccaggtgc ggccgagggc ggcgtggggc gaccgtcggc tcgtcgaccg
      481 cgctcagagc agccggcctt ctgcggccgc cgtcgccgag gcgacggagc tgctgggact
      541 cccgtcgctc agccggtgag ctgaggcgtg ccgggcacgg cccgggccgg cgccgctggc
      601 gggcgcgtgc ccgtcaccgg tgggatcgcg ccggactcga ccacgatcac ctcgatgtcg
      661 gctcggtacg ggcaccagcc gtcgtcctcg cagaagaact cgctgaaccg gaacgtgccg
      721 agctccgtgg ccactccgtc ccaggtgccg tcgaggtgga acgcggtgcc gggcggcacg
      781 gtcccctcga cggagcgaac gaggccgtcc ggcgggctgt aggccacctc ctcgacgtcg
      841 atcgccgccg gtgtcgtgcc ccgctcgatg gtctgcgtcg tcgacacgtc gccgatgttg
      901 cagccgaggt cggcgatgcc ctggctcatc gacgtgctcg cgctccccgg cgaaccgatg
      961 gtgtagccgg cgcccgctgt gaggtcgacg gtcaggtcgc cgccggtctc cggggatgct
     1021 tcgagcacga cctgggcctc accggcggcg atggtggcct gcacgggaag gccactcgtg
     1081 gaggccagcg accctccgta ggagagggcc acgtccagcg acgccgacgc gtcaccgctg
     1141 cgtgtcaggg tgacccggat cggctcggtg atgttggtct gggtctggcc cttgcagtcg
     1201 tcggcggaga agaccgagcc gttggcggcc gtgaccgtca ccaccggatc ggctgcttgg
     1261 gccggagcgg gcgccgcgtg ggtgacgccg acggcgagga gcagcgccgc ggtgagggcc
     1321 gcgaggcggc gggagctggt tcgggcggac atcggggttc cccttcgagc gagagccggg
     1381 tgtggggtgg cgtcaacccc acgatgccgg actcccgtcg ttcgttccga ggcggcccta
     1441 ccatcggccc catggcctcc gagctcgatg ccctcctcga ccatgcagcg atcgaccggc
     1501 tgcagcgggc gtacgccgac ggcgtgacca ggcgggactg ggagcaggtc cgcggtctct
     1561 tcgtggccga tgccgccatc tcgctcgact tggtgagccg tccggccgtg cagctcgccg
     1621 gaccggtgga gctcaccgac ttcgtgtcag ccgcgctcgc gccgttctcg ttcttccagt
     1681 tccagatcgt caacagccac atcgaggcct ccaccgtcga cccggcggcg gcgaaggccc
     1741 ggatgttcat gtgcgagctg cgcctgaagc acggcgcgtc ccagcgcgac gacacctacg
     1801 gcctctaccg cgacacgtac cgcaagacgg aggaggaggg ctacggaggc tggcgcttcg
     1861 cgtcccgctg gtaccgcacc gtcgcccgct tccccgacaa cgaggtcttc ccactcgact
     1921 aggaaccgtc gtggtcgggg cgcggcacgg tccggacgcc gagcccgctg atcccggcga
     1981 ggtcatcggg gttcgtggtg caccgcggga ggccgctggc gatggcggtg gccgccggcg
     2041 agacggctca agccgtggcg tcgatgcggc gggccatcca gcgggggtcg ggggcgaagc
     2101 cgctgtcctc gtagaggggg cggccgtcgt cggtcgcgaa gaggtagagg cggcgcaagc
     2161 cccacgacgc ggcgtcgtcg atcgcggtct ggagcagcgc tcgggcgatg ccctggcgcc
     2221 tcgccgaagg cgcgatccac aggttcacga cgtagccctc g
//
LOCUS       DATMFS010000433         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_318086, whole genome shotgun sequence.
ACCESSION   DATMFS010000433 DATMFS010000000
VERSION     DATMFS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318086"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VNQ33_08225"
     CDS             complement(<1..1626)
                     /locus_tag="VNQ33_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98131.1"
                     /translation="MLAPADPRPDGRRHPLAAGRLDRRRHDRRGLLSTYLGYLFAGLG
                     GGAVVASLALGLVLTHRSSGVVNFAHAAMGMYVAFAFFEFRATGDWVLPVIGLPARVH
                     LLARPTLASALAFALIVSVLLGLVVYLLVFRPLRPAPALARVVASLGLLLYIQEIVRL
                     RFPVAGASVVTRRPVLPDEPVAVFGTTVTENRLLLAGLVVGVALLLGAVFRFTRFGLA
                     TRAGAGNEKGALLLGISPDRLASVCWAVASVLAGFAVILIEPIAGLSATTTTLLVIPA
                     LAAALLGGLESFAITTAAGLAIGMVQSLILGWSVRPDTRWIPDWIPTTGLQQLVPVLV
                     ILGGLVWRGDALPDRAAITGKRLPPSPAPRHVTAWTVALVGLASAGLLTLDSGYRHAL
                     IVSMVFALLTLSVVVLTGFSGQISLAQLAFAGVAGFAAIRLAEHRVPFPIALVLASLL
                     AAVIGVLVGWPATRVRGMSLAIATLAMAVAIEELVLASEPFSGGHAGASAPRPFLFGI
                     DVGVGATGTGNFRAAFGFVCLVVLTLASLAVVNL"
     gene            complement(1530..2846)
                     /locus_tag="VNQ33_08230"
     CDS             complement(1530..2846)
                     /locus_tag="VNQ33_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98132.1"
                     /translation="MRRTAILAATLAATVLTASVLTGCSEPAPEKKTGIGKSTTAATE
                     ACEGKALLACAGKSTIGEYVPDEPTKATGEPIQLGMINQENTAAGSYPELSQAVQAGV
                     EFINTELGGVDGRPIEVDVCNTEFSAEGSTSCGQKYVESKAPAVLGGIDVFGNGIETL
                     ADNGIPYIGGIPISFQSVRSPNSFQWSGGTWGAAVAFSWYAAEELDAEKVSIVYGDFG
                     SIADSATDAEQVLKSRGVEAQLVPYPIMATDISSALNAAAATKPDALLILAADSGCKA
                     GFDGVSSLGIDATAFYVGACAAPSITNEAGTKKTNGAIFNVEGPVGTKPPNPDFVLYS
                     AVIDKYGKGLDPVGAGTVSFRSLMNLYVILNQLDGDITPDAITKALKAQVDAPSFSGH
                     PYTCDGKQYPGLPAMCSPQQILAQMDDGTLSQLGDWIDVGTIAAGS"
     gene            2986..>3834
                     /locus_tag="VNQ33_08235"
     CDS             2986..>3834
                     /locus_tag="VNQ33_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWJ98133.1"
                     /translation="MATLTKNLFRGQGERDHALDVVEGHWPTDVEGAVYVVGPDKRAP
                     EGHWFAEAGLVEKIRLVPDRRGRIGVQHRVIDTPLQRIKRRFGFLFRRVAFMELSPFG
                     VTNLANTNIQSIDGRMFVGYDAGRPIEIDPETLRFITPVGSNAEWLQAAPGVFEPLCA
                     VAAHPASDFEEQALYFVNYTQIAPPGVPKETWLARWALDGPVQRWRVEGMSAFDSIHD
                     IKATEHHLVFSDLPFVVEPEMMRGQPRSRRIQEHTSLWIVAKEDLRRTPPGGAVRAVE
                     VRLPMPT"
BASE COUNT          594 a         1336 c         1408 g          496 t
ORIGIN      
        1 caggttgacc acggcgagcg acgccagcgt gagcaccacc aggcacacga acccgaacgc
       61 cgctcggaag ttgcccgtgc cggtggcgcc gacgccgacg tcgatcccga acaggaacgg
      121 ccggggtgcg ctcgccccgg cgtgaccgcc cgagaacggc tcgctcgcca gcaccagctc
      181 ctcgatggcg acggccatgg cgagggtggc gatggccagg ctcatcccgc gcacgcgggt
      241 ggccggccaa cccaccagca ccccgatcac cgcggcgagc agcgaggcca gcacgagggc
      301 gatcgggaac gggacgcggt gctcggcgag gcggatcgcg gcgaacccgg ccacgccggc
      361 gaaggccagc tgggcgagcg agatctgccc gctgaacccg gtgagcacca cgaccgacag
      421 cgtgagcagg gcgaacacca tcgacacgat cagcgcgtgc cggtagccgg agtccagcgt
      481 gagcaggccg gcgctggcca gaccgacgag cgccacggtc cacgccgtca cgtgccgcgg
      541 cgcgggcgag ggcgggaggc gcttgccggt gatggcagcg cggtccggga gggcgtcgcc
      601 gcgccacacc aagccgccca ggatcaccag caccggcacc agctgctgga gcccggtggt
      661 ggggatccag tccgggatcc agcgggtgtc ggggcggacc gaccagccga ggatcagcga
      721 ctggaccatg ccgatggcca ggcccgccgc cgtcgtgatg gcgaacgact ccagcccgcc
      781 cagcagcgcg gcggcgaggg cggggatcac gagcagggtc gtggtggtgg cgctcaaccc
      841 ggcgatgggc tcgatgagga tgacggcgaa cccggccagc accgaggcca ccgcccagca
      901 caccgacgcc agccggtcgg gcgagatgcc cagcaggagg gcgcccttct cgttgccggc
      961 gccggccctc gtggccaggc cgaaccgggt gaagcggaac accgccccga gcagcagcgc
     1021 caccccgacc acgagcccgg cgagcagcag ccggttctcg gtgaccgtgg tgccgaagac
     1081 ggcgacgggc tcgtcgggca gcaccggccg acgggtcacg accgacgcgc cggcgacggg
     1141 gaaccgcagc cgcacgatct cctggatgta gagcagcagg ccgagcgacg cgaccacccg
     1201 ggcgagcgcc ggcgccgggc gcagcggccg gaacaccaac aggtagacga cgagcccgag
     1261 gagcacgctg acgatgagtg cgaaggcgag cgcgctggcg agcgtggggc gggccagcag
     1321 gtggacccgg gcgggcaggc cgatgacggg cagcacccag tcgcccgtcg cccggaactc
     1381 gaagaaggcg aacgccacgt acatgcccat ggcggcgtgg gcgaagttca ccacccccga
     1441 ggagcggtgg gtgaggacca gcccgagggc gagcgatgcg accaccgcgc cgccgccgag
     1501 cccggcgaag aggtagccga ggtaggtgct caggagcccg cggcgatcgt gccgacgtcg
     1561 atccagtcgc ccagctgcga gagggtgccg tcgtccatct gggcgaggat ctgctggggc
     1621 gagcacatcg caggcaggcc ggggtactgc ttcccgtcgc acgtgtacgg gtggccgctg
     1681 aagctcgggg cgtcgacctg ggccttgagg gccttggtga tggcgtcggg cgtgatgtcc
     1741 ccgtcgagct ggttgaggat cacgtacagg ttcatcagcg agcggaacga gacggtgccg
     1801 gcgcccaccg ggtccaagcc cttgccgtac ttgtcgatga cggcgctgta gagcacgaag
     1861 tcggggttcg gcggcttggt gcccaccggg ccctcgacgt tgaagatggc gccgttcgtc
     1921 ttcttggtcc cggcctcgtt ggtgatcgac ggggcggcgc aggcgccgac gtagaacgcg
     1981 gtggcgtcga tgccgagcga cgagacgccg tcgaacccgg ccttgcagcc ggagtcggcc
     2041 gccaggatca gcagggcgtc gggcttcgtg gccgcggcgg cgttgagcgc cgagctgatg
     2101 tcggtggcca tgatcgggta cggcaccagc tgagcctcga cgccgcggct cttcaggacc
     2161 tgctcggcgt cggtggcgct gtcggcgatg gacccgaagt cgccgtagac gatcgacacc
     2221 ttctcggcgt cgagctcctc ggcggcgtac caggagaagg ccaccgccgc gccccacgtg
     2281 ccgccgctcc actggaacga gttgggcgac ctcaccgact ggaagctgat cgggatgccg
     2341 ccgatgtagg ggatgccgtt gtcggcgagg gtctcgatgc cgttgccgaa cacgtcgatc
     2401 ccgcccagca cggccggcgc cttcgactcg acgtacttct gaccgcagga ggtggacccc
     2461 tcggcggaga actcggtgtt gcagacgtcg acctcgatgg gccgcccgtc gacaccgccc
     2521 agctcggtgt tgatgaactc gacgcccgcc tgcaccgcct ggctcagttc ggggtacgag
     2581 ccggcggcgg tgttctcctg gttgatcatc ccgagctgga tcggctcgcc cgtcgccttg
     2641 gtgggctcgt cggggacgta ctccccgatg gtcgacttcc cggcgcaggc gagcagggcc
     2701 ttgccctcgc aggcctcggt ggcggccgtc gtgctcttgc cgatcccggt cttcttctcc
     2761 ggagccggct cgctgcaccc ggtgagcacc gacgcggtca gcaccgtggc cgccaacgtc
     2821 gcggccagga tcgcagttcg cctcacgttg cccccaagct ccgtgtcgtg tccccgccgg
     2881 gcgggatggt ccgggtcccc tgacctggcc ttgcgcccag atggtaaggg gtgaacactg
     2941 tttagcgcag ggcgggcggg ggtgccgccg agccgggggg ggacgatggc aacgctcacg
     3001 aagaacctgt tccgaggcca aggcgagcgc gaccacgcgc tcgacgtggt cgaagggcac
     3061 tggcccaccg acgtggaggg cgcggtctac gtggtcgggc ccgacaagcg ggcgcccgag
     3121 ggccactggt tcgccgaggc cggcctcgtc gagaagatcc gtctcgtgcc cgaccgccgg
     3181 ggccgcatcg gcgtgcagca ccgcgtgatc gacacgcccc tccagcgcat caagcgccgg
     3241 ttcgggttcc tcttccgccg tgtcgccttc atggagctgt cgccgttcgg cgtcaccaac
     3301 ctcgccaaca ccaacatcca gtcgatcgac ggccggatgt tcgtgggcta cgacgccggc
     3361 cgccccatcg agatcgaccc ggagaccctg cggttcatca cgccggtggg cagcaacgcc
     3421 gagtggctcc aggcggcgcc gggcgtgttc gagccgctgt gcgccgtggc cgcccacccc
     3481 gcctcggact tcgaggagca ggcgctctac ttcgtgaact acacgcagat cgccccgccc
     3541 ggcgtgccca aggagacctg gctcgcccgc tgggcgctcg acggcccggt gcagcggtgg
     3601 cgggtcgagg gcatgtcggc gttcgactcg atccacgaca tcaaggccac cgagcaccac
     3661 ctcgtcttca gcgacctgcc gttcgtcgtc gagcccgaga tgatgagggg ccagccccgg
     3721 agccgccgca tccaggagca cacctcgctt tggatcgtgg ccaaggagga cctccgccgc
     3781 accccgcccg gtggcgcggt ccgggcggtc gaggtgcggc tgccgatgcc gacc
//
LOCUS       DATMFS010000434         8067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_85069, whole genome shotgun sequence.
ACCESSION   DATMFS010000434 DATMFS010000000
VERSION     DATMFS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85069"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VNQ33_08240"
     CDS             complement(<1..1253)
                     /locus_tag="VNQ33_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWJ98134.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRSQVEEIIGQGGSSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLSRVRQQVIQLLSGYSGSGPQGSGGPGGEK
                     AGASTGQASGEAPSGSLVLDQFGRNLTQLAKDKKLDPVIGRARETERVMQVLSRRTKN
                     NPVLIGEPGVGKTAIVEGLAQAIAADNVPETLTGKQLYTLDLGALVAGSRYRGDFEER
                     LKKVLKEIRTRGDIIMFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLD
                     EYRKHLEKDAALERRFQPVQVGEPSVAHTIEILKGLRDRYEAHHRVSITDSALVAAAG
                     LADRYISDRFLPDKAIDLIDEAGARL"
     gene            1406..2404
                     /locus_tag="VNQ33_08245"
     CDS             1406..2404
                     /locus_tag="VNQ33_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWJ98135.1"
                     /translation="MATNPLLALPHAESDRDRVEDELRRAVAAPTEAMTEMAGHLLGA
                     GGKRVRPMFAIAAGACRPGGSDAPSPLDVVRGGVSVELVHLGSLLHDDVIDEAVTRRG
                     IESVNARWGNLRAILAGDFLLAKASEIAASLGTEVAGLLAATIGELCTGEVIELDDAY
                     NVERTEAAYLQAIEGKTASLLSTACRIGGIVAGLPRADIDRLTEFGRVYGMAFQIVDD
                     VLDVIATDEQLGKPAAHDLVEGAYNLPTLRALAGPQGDELRAILGAPIEGEAWTQARD
                     LVRQGGAVDSAIDTAREYVAEACDLLAPFEEHPAAIGLQGASRHLIDGLEAVRTPA"
     gene            complement(2457..3467)
                     /locus_tag="VNQ33_08250"
     CDS             complement(2457..3467)
                     /locus_tag="VNQ33_08250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWJ98136.1"
                     /translation="MTLAFTGDLLPHLPLVAVARQYGQQHGDRYDFKPMLDPMRPVIE
                     GADLAICHLEVPAAANQDRLSGYPTFGGPVELIDAVKVTGYDGCSTASNHSLDQGRAG
                     IGVTLDRFDQDGLQHAGTARTAEEGAATTMYDVGGAKVAHLSYAYGFNGYQLPVDAPF
                     AANQIDVNRIHADATRARQAGAQLVVVSLHWGTEYQHEPDAFQRQTAADLLPWPDIDV
                     IVGHHAHVVQPIERVEGTYVVWGLGNHLANQPQIPKSDGLTAVLSATKGAGGRYRISQ
                     IVAVPTWIEPGTFRVIPIPNALQDPNLSGSLRSQLTASYDRTAAVLHTTPTNGVQIAP
                     KP"
     gene            3451..4878
                     /locus_tag="VNQ33_08255"
     CDS             3451..4878
                     /locus_tag="VNQ33_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ98137.1"
                     /translation="MNARVIDSGPGDGGSVVVVVLGGAVVDVVVPLADALAAVPVGAA
                     GVVDVARSAAGAGLPAQPARSPSTAAATRAPALCVARPLTLRTLRHPSWIGSDPYASP
                     MAASAPTDRRVVTDLEWGVTRGIAGFRWLALGWAAIVLYLTRADVDGMVLGIALLGVG
                     LVVTAVATEASIRRRAWAFAPGAVVVELGVGASMLLLDGFVYDRANHSQSLGSAWPVA
                     GILMAGIVGGPVAGSVAGVALGIARWLGAEIAPFSGEVSSLSLVSSGVLFAIAGAAAG
                     AAMDLVRRAESAIAEARARDEVARTLHDGVLQTLAVVQRRSSDDELVVLAREQELELR
                     SYLAGDRPQTEEIGAALRSTAARIERRDGIRVEVLFVDDDPPFVAPAVVEAIAGAVGE
                     ALTNAHKHGHATRATVFVDIDDEVFCSVKDDGQGFDPASVPAGTGIAQSIEGRLAEVG
                     GSAEVDARPGSGTEVRLHAPLRPKR"
     gene            5075..5707
                     /locus_tag="VNQ33_08260"
     CDS             5075..5707
                     /locus_tag="VNQ33_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ98138.1"
                     /translation="MGELRAVRVVVADDHQIWRSGLRADLGEAFAVIGEAGDADEAIA
                     VIGREKPDLVVCDLHMPGGGGIKVAKECGEQVPIVMLTVSEAERDLLDAVAAGAQGYL
                     VKSTPVDELRTALYQAAQGEPVFSPSLAALVLGEFRRMHKGAGGTQALSDREREVLQY
                     VARGHTYKEIGEQLFIAEKTVENHVRNILAKLHLNRKQELIRYALDHGIE"
     gene            complement(5933..6691)
                     /locus_tag="VNQ33_08265"
     CDS             complement(5933..6691)
                     /locus_tag="VNQ33_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ98139.1"
                     /translation="MIHGLGADTRGWVMQKRALGSRFRLVMVDNRGVGRSDRAEGPYD
                     LEVMATDALAALDHAGYGSAHVIGASMGGIISQVIGVRHPERVRSLTLACTACRHFTW
                     RKELLSEWADQAQAYGMREFVRRNLKWMVGPRSLRRTWPAMAVLGPLAFNVPVASFVA
                     QIQAILSIEDSLRGELADVTAPTLVLCGSQDVLTTQGDSEEIASLIPAAELAVVRGGA
                     HLFMVEQAGSFNRAVNGFLTKVTDPDRFLALQNA"
     gene            complement(7053..7721)
                     /locus_tag="VNQ33_08270"
     CDS             complement(7053..7721)
                     /locus_tag="VNQ33_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98140.1"
                     /translation="MSASLLRRGVPGVQALAQVQRRARGAYDVDEWGLDPELVAMANP
                     VFGLRWDIEVRGAEQLPVVGGAVLVFNRRFGISEPWIVARGIRQATGRFVRTVGVPDV
                     TPIGPFIRRFGGVLDRTDEIAGLLRSGQLVGLPMSRDVRSRERVGHLEVERLEAAIET
                     SAPVVPVALVGRELGRAWTIVVGEPIPPVARGGPLAAAELAESTHRACQVLLDESLPT
                     SWWF"
     gene            complement(7718..>8067)
                     /locus_tag="VNQ33_08275"
     CDS             complement(7718..>8067)
                     /locus_tag="VNQ33_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98141.1"
                     /translation="ACQAMVAAASAGHDGPLNVVGPGAVTASQAVRMGRRLPLPVFGP
                     GWWAAAAIAELQGAPLPPHSRELLTRGCTADGALARSVLGVDPRSTHDVVTQLYRWDA
                     LAHPEPSNQVTSR"
BASE COUNT         1096 a         2865 c         2967 g         1139 t
ORIGIN      
        1 agtcgcgcgc cggcctcgtc gatcaggtcg atcgccttgt ccggcaggaa gcggtccgag
       61 atgtaccggt cggccagccc cgcggcggcg accagcgccg agtcggtgat ggacacccgg
      121 tggtgcgcct cgtaccggtc gcgcaggccc ttgaggatct cgatggtgtg cgccaccgac
      181 ggctcgccca cctgcaccgg ctggaagcgc cgctccaggg cggcgtcctt ctccaggtgc
      241 ttgcggtact cgtcgagggt ggtcgcgccg atggtctgca gctcgccgcg ggccagcatc
      301 ggcttcagaa tgctcgccgc gtcgatcgcg ccctcggcgg cgcccgcgcc gaccagggtg
      361 tggatctcgt cgatgaacat gatgatgtcg ccgcgggtgc ggatctcctt gagcaccttc
      421 ttcaggcgct cctcgaagtc gccccggtag cgggagccgg cgacgagggc accgaggtcc
      481 agcgtgtaga gctgcttgcc ggtgagcgtc tcgggcacgt tgtcggccgc gatggcctgg
      541 gccaggccct cgacgatggc ggtcttgccg acgccgggct cgccgatcag caccgggttg
      601 ttcttggtgc ggcggctgag cacctgcatg acccgctcgg tctcgcgggc gcgcccgatc
      661 accgggtcga gcttcttgtc cttggcgagc tgggtgaggt tgcgaccgaa ctggtccagc
      721 acgagcgacc ccgacggcgc ctcgccgctg gcctggccgg tggacgcgcc cgccttctcg
      781 ccgcccggcc cacccgagcc ctggggaccc gagcccgagt agcccgacag gagctggatg
      841 acctgctggc ggacccggct gagatcggcg cccagcttca ccagcacctg ggcggccacg
      901 ccctcgccct cacggatgag gccgaggagg atgtgctcgg tgccgatgta gttgtggccg
      961 agctgcagcg cctcccgcaa ggagagctcc agcaccttct tggcgcgcgg ggtgaagggg
     1021 atgtggcccg acggggacga cccgccctgg ccgatgatct cctcgacctg gctgcgcacg
     1081 gcctccagcg agatcccgag cgactcgagg gccttggcgg ccacaccctc gccctcgtgg
     1141 atcagtccga gcaggatgtg ctcggtgccg atgtagttgt ggttgaggag gcgggcctcc
     1201 tcctgtgcca ggaccaccac tcggcgggcc cggtctgtaa agcgctcgaa caagttcctg
     1261 cctcccaatc agcgggattc gtggggcgag tgtaccgctc ctcccccgga accccgtcgg
     1321 cagaaggacg ctcgtgctga tcggcccagg tgagcgcacc ggttcgggct tcagcacggg
     1381 ggcggtggcc ccatagcctg cggagatggc gacgaacccc ctgctggcgc tcccccatgc
     1441 cgagtccgac cgcgaccggg tggaggacga gctgcgccgg gcggtggccg cccccaccga
     1501 ggccatgacc gagatggcgg ggcacctgct cggggcgggc ggcaagcggg tccggccgat
     1561 gttcgccatc gccgcgggcg cctgccggcc gggcgggtcc gacgccccgt cacccctcga
     1621 cgtggttcgg ggcggggtgt ccgtcgagct cgtccacctc gggtcgctcc tccacgacga
     1681 cgtgatcgac gaggctgtga cccgtcgcgg catcgagagc gtcaacgccc ggtggggcaa
     1741 cctccgcgcc atcctggccg gcgacttcct gctggcgaag gcgtcggaga tcgcggcgtc
     1801 gctcggcacc gaggtggccg ggctgctcgc cgccaccatc ggcgagctgt gcaccggcga
     1861 ggtcatcgag cttgacgatg cgtacaacgt cgagcgcacc gaggccgcgt acctccaggc
     1921 catcgagggc aagaccgcct cgctgctctc caccgcctgc cgcatcggcg ggatcgtggc
     1981 cggcctccca cgcgctgaca tcgaccggct caccgagttc ggccgggtct acggcatggc
     2041 gttccagatc gtggacgacg tgctcgacgt gatcgccacc gacgagcagc tcggcaagcc
     2101 cgccgcgcac gatctggtcg agggcgccta caatctgccc accctccgtg ccctcgccgg
     2161 gccgcagggc gacgagctgc gcgccatcct cggcgccccc atcgagggcg aggcgtggac
     2221 ccaggcacgt gacctcgtcc gccagggcgg cgccgtcgac agcgccatcg acacggcccg
     2281 cgagtacgtg gccgaggcgt gcgacctcct ggctccgttc gaggagcacc ccgccgccat
     2341 cggcctccaa ggcgccagcc gccacctcat cgacggcctc gaagccgtcc gcacccccgc
     2401 ctgagcggtt ctcgtcgccc cacgcgctcg aaccgagcca cgacgagcaa acgacgtcag
     2461 ggcttcgggg cgatctggac gccgttggtg ggggtggtgt ggaggaccgc cgcggtgcgg
     2521 tcgtagctgg cggtcagctg ggagcggagg ctgcccgaca ggttcgggtc ctggagggcg
     2581 ttcgggatgg ggatgacgcg gaaggtgccg ggctcgatcc acgtcgggac ggccacgatc
     2641 tggctgatcc ggtagcggcc tccggcgccc ttggtggccg agagcaccgc cgtcagaccg
     2701 tcgctcttgg ggatctgcgg ctggttggcg aggtggttgc cgaggcccca gaccacgtac
     2761 gtgccctcga cccgctcgat gggctgcacg acgtgggcgt ggtggccgac gatcacgtcg
     2821 atgtcgggcc acggcagcag gtcggcggcg gtctggcgct ggaaggcgtc gggctcgtgc
     2881 tggtactcgg tgccccagtg caggctcacg accacgagct gggcacccgc ctgccgagcc
     2941 cgggtggcgt cggcgtggat gcggttcacg tcgatctggt tggcggcgaa gggagcatcg
     3001 accgggagct ggtagccgtt gaagccgtag gcgtagctga ggtgggcgac cttggccccg
     3061 ccgacgtcgt acatcgtggt ggccgcaccc tcctcggccg tgcgggcggt gccggcgtgc
     3121 tgcaacccgt cctggtcgaa ccggtcgagc gtcacgccga tgccagctcg gccttggtcc
     3181 aggctgtggt tcgacgccgt cgagcagccg tcgtagccgg tcaccttcac ggcatcgatc
     3241 agctccaccg ggccgccgaa ggtcgggtac cccgagagcc ggtcctggtt cgccgccgcc
     3301 gggacctcga ggtggcagat ggcgaggtcg gcgccctcga tgaccgggcg catcgggtcg
     3361 agcatcggct tgaagtcgta gcggtcgccg tgctgctggc cgtactgccg ggcgacggcc
     3421 acgagcggca ggtgcgggag caggtcgccg gtgaacgcga gggtcatcga ctcgggtccg
     3481 ggcgatggcg gctccgtcgt cgtggtggtc ctcggcggcg ctgtggtcga cgtcgtggtg
     3541 ccgctcgccg acgcgctcgc cgcggtgccg gtcggcgccg cgggcgtggt cgacgtcgcg
     3601 cgctcggcag ccggtgccgg gctgcccgcg cagccggcga ggagcccgag cacggccgcc
     3661 gcgacgaggg ctcccgccct ctgcgtcgct cggcccctca cgctgcgaac gctacggcac
     3721 ccctcgtgga tcggatcgga cccctacgct tcgcccatgg cggccagcgc tccgaccgac
     3781 cggcgggtcg tcaccgacct cgagtggggc gtgactcgtg gcatcgccgg cttccggtgg
     3841 ctggcgctcg gctgggccgc catcgtcttg tacctgacgc gggccgacgt cgacggcatg
     3901 gtgctcggca tcgcgctcct cggggtcggc ctcgtcgtga cggccgtcgc caccgaagca
     3961 tcgatccgcc gccgtgcgtg ggcgttcgct cctggcgccg tcgtcgtcga gctgggcgtc
     4021 ggcgcctcga tgctcctgct cgacggcttc gtctacgacc gcgccaacca cagccagagc
     4081 ctcggctcgg cctggccggt ggccgggatc ttgatggccg gcatcgtcgg cgggccggtc
     4141 gccggttcgg tcgcgggtgt cgccctcggc atcgcccgct ggctcggcgc cgagatcgct
     4201 cccttctccg gcgaggtctc ctcgctctcg ctcgtgtcga gcggcgtgct gttcgccatc
     4261 gccggcgcgg cggcgggggc ggccatggac ctcgtccgac gggcggaatc ggccatcgcc
     4321 gaggcccgcg cccgagacga ggtggcccgc accctccacg acggcgtgct ccagaccctc
     4381 gccgtcgtcc agcgtcgttc gagcgacgac gagctggtgg tgctcgcccg cgagcaggag
     4441 ctcgagctgc ggtcctacct cgccggcgac cggccccaga ccgaggagat cggcgctgcc
     4501 ctgcgcagca ccgcagcccg catcgagcgg cgcgacggga tccgggtcga ggtgctcttc
     4561 gtcgacgacg acccgccgtt cgtcgccccg gccgtggtcg aggccatcgc cggcgccgtc
     4621 ggcgaggccc tcaccaacgc ccacaagcac ggccacgcca cccgggccac ggtcttcgtg
     4681 gacatcgacg acgaggtgtt ctgctcggtg aaggacgacg gccagggctt cgacccggcc
     4741 tcggtccccg ccggcaccgg catcgcccag tcgatcgagg gccgcctggc cgaggtcggc
     4801 ggctccgccg aggtcgacgc ccgccccggc tccggcaccg aggtccgcct ccacgccccc
     4861 ctccgcccca agcgctgacc gaacccgcgc cgcctccaca aagccgaact tttcgtgatc
     4921 gtggtggccc tgccaccaac ttcacgaaaa gttcgtgggg gtgggggtgg ggtggggggt
     4981 ggtggggtgg gtacctcacg gcccctcggc accgccgccc gcctccagga gctggtcgcg
     5041 ggttcgtgcc aggccgaacg ggcagactgg gccgatgggt gagcttcggg cggtgcgggt
     5101 ggtcgtcgcc gacgatcacc agatctggcg atccggtctg cgagccgacc tcggcgaggc
     5161 gttcgccgtg atcggcgagg cgggcgatgc cgacgaggcc atcgccgtca tcggccggga
     5221 gaagcccgac ctcgtggtgt gcgacctgca catgccgggc ggtggcggca tcaaggtggc
     5281 caaggagtgc ggcgagcagg tgccgatcgt gatgctcacc gtgtccgagg ccgagcgcga
     5341 cctgctcgat gccgtggccg ccggcgccca gggctacctg gtcaagagca cgccggtcga
     5401 cgaactgcgc accgcgctct accaagcggc gcagggcgag cccgtcttca gcccgagcct
     5461 cgccgccctc gtgctcggcg agttccggcg catgcacaag ggggcgggcg gcacccaggc
     5521 cctgtccgac cgggagcgcg aggtgctcca gtacgtggcc cgcggccaca cctacaagga
     5581 gatcggcgag cagctgttca tcgccgagaa gacggtcgag aaccacgtcc gcaacatcct
     5641 cgccaagctc cacctcaacc ggaagcagga gctcatccgc tacgcgctgg accacggcat
     5701 cgagtagccg gcctgcggcc tctcgtcact cgttcggctg cgcctcgctc cgttcctcga
     5761 atcgatcgac gggctgacgc ccggcacctt cgcatctcgg cacgactccg caagctccgt
     5821 cgcacctcgc tggctcgaag gggctccgca agctccaccc cccaaccccc cccggactcg
     5881 ctttcgctca gaacccaccc tcgcgctcgc ggttcgagcg acgccggggg ggtcaggcgt
     5941 tctggagggc gaggaagcgg tcggggtcgg tgaccttcgt caagaagccg ttgacggctc
     6001 ggttgaagga cccggcttgt tcgaccatga agaggtgggc gccgccgcgg accacggcca
     6061 gctcggcggc ggggatcagc gaggcgatct cctcgctgtc gccctgagtg gtgagcacgt
     6121 cctggcttcc gcacagcacg agcgtgggcg cggtgacgtc ggccagctcg ccccgcagcg
     6181 agtcctcgat cgagaggatc gcctggatct gggcgacgaa cgacgcgacg ggcacgttga
     6241 aggcgagcgg cccgagcacg gccatggccg gccaggtccg ccgcagcgaa cggggcccga
     6301 ccatccactt caggttccgg cgcacgaact cgcgcatccc gtaggcctgc gcctggtcgg
     6361 cccactcgct gagcagctcc ttgcgccagg tgaagtggcg gcacgcggtg caggcgaggg
     6421 tgagcgaccg gacccgctcg gggtggcgca cgccgatcac ctggctgatg atcccgccca
     6481 tcgaggcacc gatgacgtgc gccgatccgt agccggcgtg atccaaggcg gcgagggcgt
     6541 cggtggccat cacctcgagg tcgtagggcc cctcggcccg gtcggaccgg ccgacgccgc
     6601 ggttgtcgac catcaccagc cggaagcggc tcccgagcgc ccgcttctgc atgacccagc
     6661 cccgggtgtc ggcgccgagc ccgtggatca tcagcagcgg ctccccatcc cgacgcccat
     6721 cgagcgcata cgagatcttc gtcccatcct caacgaccac agacggcatc aggcaacccc
     6781 ccgggagatg ctgagcgaag agcgcgagcg aggggctccg agcgactcga tcccccgacg
     6841 aagtgcgctg cgacgccacg aggacggggt tccgagcgaa gcgagctcgg ggggtttggg
     6901 gggcagagct tgcggagccc cccaagacag cgaggtgcga cgcagtcgtg ccgagctgcg
     6961 aagacgtcgg gcgtcagccc gtccccgagt tcgaggagcg gagtgaggcg cagccgaacg
     7021 agtgacgaga ggaggagcgc agcgacgacc catcagaacc accagctcgt ggggagggac
     7081 tcgtcgagga ggacctggca ggcgcggtgg gtggactcgg ccagctcggc ggcggcgagg
     7141 gggccgccgc gagcgacggg cgggatcggc tcacccacca cgatggtcca ggcgcggccc
     7201 agctcccggc cgacgagggc gacggggacg acgggtgcgc tggtctcgat ggccgcttcc
     7261 aggcgctcga cctcgaggtg gccgacgcgc tcgcggctgc ggacgtcgcg cgacatgggc
     7321 agtccgacga gctgaccgga gcggaggagc ccggcgatct cgtcggtgcg gtcgagcaca
     7381 ccgccgaagc ggcggatgaa cgggccgatg ggggtcacgt cggggacgcc gaccgtgcgc
     7441 acgaaccggc cggtggcctg gcggatgccg cgtgcgacga tccacggctc gctgatcccg
     7501 aagcggcggt tgaacacgag cacggcacca ccgacgacgg gaagctgctc ggcgccgcgc
     7561 acctcgatgt cccagcgcag tccgaacacc gggttggcca tggcgacgag ctccgggtcg
     7621 aggccccact cgtcgacgtc gtaggcgccg cgggcccggc gctggacctg ggccagtgcc
     7681 tgcacgccgg gcacgccgcg tcgcaggagg ctcgcactca ccgggaggtc acctggttcg
     7741 agggctcggg gtgggcgagg gcatcccacc ggtagagctg ggtgacgaca tcgtgggtgg
     7801 atcggggatc gacgccgagc acgctccggg ccagggcgcc gtcggccgtg cacccgcggg
     7861 tgagcagctc gcgggagtgc ggcgggagcg gggcgccctg cagctcggcg atggccgcgg
     7921 ccgcccacca gcccggaccg aacaccggca gggggaggcg acggcccatg cgcaccgcct
     7981 ggctcgcggt gacggcgccg gggccgacca cgttgagggg gccgtcgtgg ccggcggacg
     8041 ccgcagccac catcgcctgg caggcgt
//
LOCUS       DATMFS010000435         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_294785, whole genome shotgun sequence.
ACCESSION   DATMFS010000435 DATMFS010000000
VERSION     DATMFS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294785"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VNQ33_08280"
     CDS             complement(<1..395)
                     /locus_tag="VNQ33_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWJ98142.1"
                     /translation="MPKAVGIDLGTTNSVVSVLEAGDPIVIPNAEGSRTTPSVVAFAK
                     NGEVLVGEVAKRQAITNPDRTIRSVKRHMGTNWSIDIDGKAYTSQEISARTLMKLKRD
                     AEAYLGDTVTQAVITVPAYFDDAQRTATK"
     gene            818..1630
                     /locus_tag="VNQ33_08285"
     CDS             818..1630
                     /locus_tag="VNQ33_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HWJ98143.1"
                     /translation="MGDPTTDFVAMLDLEPHGPDVWVGTGPRYPWGGLYGGQIVAQAL
                     RAAASTVEPEFRVHSLHAYFIRRGDHTEPVRYEVDRVRNGRSFVTRTVTARQATGAIL
                     SVSVSFQVDEEAPEVQIAELPDAPKPSELHQDSWSSMFERATVPVEELAGRSRGWMRI
                     NADLGDDPVLNACALAYMSDDYPTDAVVALRPDRPGPDAPPDQHPFMAFSLDHAIWFH
                     RPIRAEEWHLTAMICHGLISSRGLTVGHVFTEDGIHVASVSQEVLLRRNRNV"
     gene            1710..>2323
                     /locus_tag="VNQ33_08290"
     CDS             1710..>2323
                     /locus_tag="VNQ33_08290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWJ98144.1"
                     /translation="MTSTLVLLRHGQSTWNAQNLFTGWWDADLSEQGREEAAAAGTLM
                     ADAEIVPDKVHTSLQTRAIRTANLALDEMGLLWVPVQRHWRLNERHYGDLTGRNKADA
                     TAQFGEDQVKVWRRSYDTPPPAIAADNEFNPNTDARYAAVPADVLPQSECLADVVDRM
                     LPYWFDVIVPDLAGGRTVLIAAHGNSLRALVKHLDGISDDDIAGL"
BASE COUNT          333 a          868 c          765 g          357 t
ORIGIN      
        1 tccttggtgg cggtgcgctg ggcgtcgtcg aagtaggccg gcacggtgat gacggcctgg
       61 gtcacggtgt cgcccaggta cgcctcggcg tcgcgcttga gcttcatgag cgtgcgagcg
      121 gagatctcct ggctcgtgta cgccttgcca tcgatgtcga tggaccagtt ggtgcccatg
      181 tggcgcttga cggagcggat ggtgcgatcg gggttggtga tcgcttggcg cttggcgacc
      241 tcgcccacga ggacctcgcc gttcttggcg aaggccacca ccgacggcgt cgtgcgactg
      301 ccctcggcgt tggggatgac aatggggtcg cccgcctcca agacgctgac gaccgagttg
      361 gtggtgccga gatcgatgcc gacggccttg ggcatgggta ctgctcctct gtctggggtc
      421 tgggcttggc gggtttcgga cccaccgaga tcgtgcgaac gggtacgagt ataaaagttg
      481 agtgtctggt tatcaactcc gatgccgtgt cgtccgtcat acccctgacc tggggccttc
      541 cgctggctgg gcctgcgtgc ccccgttgtg ggctgcatcg cagccgtcat cggtcgctgc
      601 tcatgggctc gggtcgccga acccacgcgc ccagggccga ccggtttgca cctcgccgct
      661 catctcacgg tccgtgcgat tcgggccgga ccccgcggat ttcgggtcac gtgtgcacct
      721 ccacggtgca caggtgaccc gaaatcgcgt cgatcgccgc caccggcgtg acgctcggca
      781 cccgggccct cgtcaccacg cgtggcacca tgaccgcatg ggcgatccga cgaccgactt
      841 cgtggccatg ctcgacctcg agccgcacgg acccgacgtc tgggtcggca ccgggccccg
      901 ctacccgtgg ggcggcctct acggcgggca gatcgtggcc caggccctgc gcgccgccgc
      961 cagcaccgtc gagcccgagt tccgggtcca ctccttgcac gcctacttca tccgccgcgg
     1021 cgaccacacc gagccggtcc gctacgaggt cgaccgggtc cgcaacgggc gctcgttcgt
     1081 gacccggacg gtcaccgccc gccaggccac cggcgccatc ttgtcggtgt cggtcagctt
     1141 ccaggtcgac gaggaggcgc ccgaggtcca gatcgccgag ctgcccgacg cgccgaagcc
     1201 gagcgagctg caccaggact cgtggagctc gatgttcgag cgggccacgg tgcccgtcga
     1261 ggagctggcc ggccggtcgc ggggatggat gcgcatcaac gccgacctgg gcgacgaccc
     1321 ggtcctcaac gcctgcgccc tcgcctacat gtccgacgac taccccaccg atgccgtggt
     1381 cgccctccgc ccggatcggc ccggccccga cgcgccgccg gaccagcacc cgttcatggc
     1441 gttcagcctc gaccacgcca tctggttcca ccggccgatc cgtgccgagg agtggcacct
     1501 cacggcgatg atctgccacg gcctcatctc ctcgcgcggg ctcaccgtcg gccacgtgtt
     1561 caccgaggac ggcatccacg tcgcgtcggt cagccaggag gtcctgctcc gccgcaaccg
     1621 caacgtctga cccggcggcg cggcaccgtg ccgagccgag cgacgcccag atggcgggcg
     1681 cggcagggcg cgccgtaccc tcgccggtca tgacgtcgac cctcgtcctc ctccgccacg
     1741 gccagagcac ctggaacgcc cagaacctct tcaccggatg gtgggatgcg gacctctccg
     1801 agcagggccg ggaggaggcc gccgccgcag gcacgctcat ggccgacgcg gagatcgtgc
     1861 ccgacaaggt ccacacgtcg ctgcagaccc gggccatccg caccgccaac ctggcgctcg
     1921 acgagatggg cctgctgtgg gtcccggtcc agcgccactg gcggctcaac gagcgccact
     1981 acggcgacct caccggtcgc aacaaggccg acgccaccgc ccagttcggc gaggaccagg
     2041 tgaaggtgtg gcgccgcagc tacgacacgc cgccgcccgc catcgccgcc gacaacgagt
     2101 tcaacccgaa caccgacgcc cgctacgccg ccgtccccgc cgacgtgctc ccgcagtccg
     2161 agtgcctcgc cgacgtggtc gaccgcatgc tcccgtactg gttcgacgtg atcgtcccgg
     2221 acctcgccgg cggccgcacc gtgctcatcg ccgcccacgg caacagcctc cgggcgctcg
     2281 tcaagcacct cgacggcatc agcgacgacg acatcgccgg cct
//
LOCUS       DATMFS010000436         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_131595, whole genome shotgun sequence.
ACCESSION   DATMFS010000436 DATMFS010000000
VERSION     DATMFS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131595"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VNQ33_08295"
     CDS             complement(<1..694)
                     /locus_tag="VNQ33_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98145.1"
                     /translation="MTDTPAPTDVLDGTDEPEPAAPQDGPPPSDEAPLPWWAQRRIDW
                     RSVLSVAVVGVVLAIPMRALMRYQGPPMEEGFMLVFPERILHGDMPHRDFLHLYGPGS
                     LWALAGWYKLVGVSITAERVFGLAQLAGIVFGVMALTWAWGRRVAATAGIFGVLLTTT
                     AIGLTALAWDGAVAFLVASVWVGLRARRWLTDADDPPSTLAQSRAARLLVASGALAGA
                     ALLFRPDAVVATT"
     gene            complement(691..>2689)
                     /locus_tag="VNQ33_08300"
     CDS             complement(691..>2689)
                     /locus_tag="VNQ33_08300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ98146.1"
                     /translation="AQLYRAAVVLLTHPYDSGVDRGSTYTQLGAWLPNHLDMFAAGMA
                     LAVIVVERDDRGPGRLIGRRFDAVYSRRWAAGASVLLGVVVLWLAGYAFGLSRTDLSY
                     GQAGEFVRHVAYLAVALALVLPCVFGRAAGGGWRWFLATPPMQFLGRISYGIYLWQIL
                     VIGRWVSSPFTPGGPDPARHPGMQFNVSFWTTLAWTVAVTVLLSAISWYLVERPALRY
                     KDKPLGVFLGGVWAVSLVSFATRVVTFGTVMERNPGNGDPFYYHAQANMLADGVGFGE
                     PIQWLTQGKFVASAIHPPLFTLWLTPASLIGARGYLSHKTMAALAGVLVIVVAALLAR
                     RLAGDRAGVVAAVLVAVYPNLFVIDGSLWPEGLYTATVGLALVFAYRWKDRPSYLRAG
                     LVGAAVGAAILTRGEALLLLPLLCLPLAISGRKLAPRWWLHAGLMGVVALGILAPWTI
                     RNLVSFEEPVPVSTNSDEVLFYANCPDVYSGSLIGYWSFNCQEKVRQERIAAGLPADP
                     PGDESQRAKGWGDLGKEYATAHKDRWPAVATARVLRVWDLRYGETNAKVLQFEGRPYD
                     WSIRGLWLWRLMAVPGIAGLVILRRRRVMVWPLVTMLAMVTLTAVAVYGHIRFRTAGD
                     LVVIVGAAVAVDAVLPGRRAPVEPDEAPAEPEPQPAPAETP"
BASE COUNT          433 a          931 c         1028 g          297 t
ORIGIN      
        1 gcgtggtggc gacgacggca tcggggcgga acagcagggc cgcgcccgcc agggctccgc
       61 tggcgaccag gagccgagcg gcccggctct gggcgagcgt cgacggcggg tcgtcggcat
      121 cggtgagcca gcgccgggcg cgcagcccga cccagaccga agccacgagg aaggcgacgg
      181 cgccgtccca cgccagggcc gtgaggccga tggccgtggt ggtcaggagc accccgaaga
      241 tgccggccgt ggcggccacc cgacggcccc aggcccaggt gagggccatg acgccgaaga
      301 cgatgccggc gagctgggcc aggccgaaga cccgctcggc ggtgatggag acgccgacga
      361 gcttgtacca accggccagc gcccacagcg accccggccc gtacaggtgg aggaagtccc
      421 ggtgcggcat gtccccgtgc aggatccgct cggggaacac gagcatgaac ccctcctcca
      481 tcggcgggcc ctggtagcgc atgagcgccc gcatcgggat ggccaagacg acgcccacga
      541 ccgccaccga cagcaccgac cgccagtcga tccggcgctg cgcccaccac gggagcggcg
      601 cctcgtccga cggcggcggg ccgtcctgcg gagcggccgg ctcgggctcg tcggtgccgt
      661 cgaggacgtc ggtgggtgcg ggggtgtcgg tcatggggtc tcggcggggg cgggctgcgg
      721 ttcgggttcg gccggggcct cgtcgggctc gaccggggct cggcgtccgg ggagcacggc
      781 gtcgaccgcg accgcggccc cgacgatgac gacgaggtcg ccggccgtcc ggaagcggat
      841 gtgaccgtag acggcgaccg cggtcagggt caccatcgcg agcatggtga cgagcggcca
      901 caccatcact cggcgccgtc gcaggatcac gagcccggcg atgccgggga ccgccatgag
      961 gcgccacagc cagagcccac ggatcgacca gtcgtagggc cggccctcga actggagcac
     1021 cttggcgttc gtctcgccgt agcggaggtc ccagacccgc aggacccgtg ccgtggcgac
     1081 cgcgggccag cggtccttgt gggcggtggc gtactccttg ccgaggtcgc cccagccctt
     1141 ggcccgctgg gattcgtcgc cgggcggatc ggcggggagc ccggcggcga tgcgctcttg
     1201 gcgcaccttc tcctggcagt tgaaggacca gtagccgatg aggctgcccg agtagacgtc
     1261 cgggcagttg gcgtagaaga gcacctcgtc gctgttcgtg gacaccggca ccggctcctc
     1321 gaagctgacg aggttccgga tggtccacgg cgccaggatc ccgagggcca caacacccat
     1381 caggccggcg tgcaaccacc agcgcggcgc cagcttccga ccagagatgg cgaggggcag
     1441 gcacagcagc ggcaacagca ggagcgcctc gccgcgggtg aggatggcgg cgccgacggc
     1501 cgctcccacc agccccgctc ggaggtacga cggccggtcc ttccatcggt aggcgaacac
     1561 gagggccagg ccgacggttg cggtgtagag gccctcgggc cagaggctgc cgtcgatgac
     1621 gaagaggttc ggatagacgg cgacgaggac cgcggccacg acgccggcgc ggtccccggc
     1681 cagccgtcgg gcgagcaggg cggccacgac gatcaccagc acgccggcca gcgccgccat
     1741 cgtcttgtgg gagaggtagc cgcgagcccc gatgagcgag gccggcgtga gccacagcgt
     1801 gaacagcggc gggtggatgg ccgaggccac gaacttgccc tgcgtcagcc actggatggg
     1861 ctcgccgaag cccacgccgt cggcgagcat gttcgcctgg gcgtggtagt agaacgggtc
     1921 gccgttgccc gggttgcgct ccatcaccgt gccgaacgtg acgacccggg tggcgaagct
     1981 gacgagcgac acggcccaca cgccaccgag gaagaccccg agcggcttgt ccttgtagcg
     2041 cagcgccggg cgctccacca ggtaccagct gatggccgac agcaggaccg tgaccgccac
     2101 cgtccaggcg agcgtcgtcc agaacgagac gttgaactgc atcccgggat ggcgcgccgg
     2161 gtcggggcct cccggcgtga acgggctgct cacccagcgg ccgatgacga ggatctgcca
     2221 caggtagatg ccgtaggaga tgcggcccag gaactgcatc ggcggcgtcg ccaggaacca
     2281 gcgccagccg cctcccgcgg cgcggccgaa cacgcagggc agcacgaggg cgagggccac
     2341 ggccaggtag gcgacgtggc ggacgaactc gcccgcctgg ccgtagctca ggtcggtgcg
     2401 ggagaggccg aaggcgtacc cggccagcca gaggacgacc acgcccagga gcacggacgc
     2461 gccggcggcc caccgccgcg agtagacggc gtcgaaccgc cggccgatca gccggccggg
     2521 accgcggtcg tcgcgctcga cgacgatcac ggcgagggcc atgccggcgg cgaacatgtc
     2581 gaggtggttc gggagccacg ccccgagctg ggtgtaggtg ctcccccggt cgacccccga
     2641 gtcgtacggg tgggtgagca ggaccacggc ggcccggtag agctgggcg
//
LOCUS       DATMFS010000437         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_294804, whole genome shotgun sequence.
ACCESSION   DATMFS010000437 DATMFS010000000
VERSION     DATMFS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294804"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..738)
                     /locus_tag="VNQ33_08305"
     CDS             complement(139..738)
                     /locus_tag="VNQ33_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWJ98147.1"
                     /translation="MDVTGLAIAAAGAAILGGSIVGARSPTIHPAEARAFRFVNSWPD
                     WLYVPLWLPMQLGNLLVGTVVGLVIAWVLGAIEVAVAVILAMALKLVVERVVRRELSQ
                     YMEVRQRPGTSEPGARLRGADVPHEGMSFPSGHVILVAAVGTVLTGHLDAGVALIPFL
                     LAALVAVGRVFVGAHNPLDVTAGMGAGLLVGGILTALLS"
     gene            1018..1425
                     /locus_tag="VNQ33_08310"
     CDS             1018..1425
                     /locus_tag="VNQ33_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWJ98148.1"
                     /translation="MLATEWGTGQVLLSMLYFTMFFIWIWLLIMVFSDIFRSHDLSGV
                     AKFVWIFFVILLPYLGVFVYLIARGHKMGEHAVADAKARDEAAQAYIRSAVGTTSGGH
                     STAAELEKLAALRDSGVLTADEFEAQKAKLLAG"
     gene            1547..>2799
                     /locus_tag="VNQ33_08315"
     CDS             1547..>2799
                     /locus_tag="VNQ33_08315"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWJ98149.1"
                     /translation="MTEHRSPVGRVMGALVPTVVDAVDPDALIDRLDIDALMERVDVD
                     ALITRVDLDALLTRIDLDALLGRIDLDALLARIDVGALIARMDMNEVIGQVDMNAVIG
                     QVDLNALLDDVDIKAVVAKAGIDEIVAEASTGVITRTLDLARRQLVGIDIIIMGAVDR
                     IFRRQRPALPDTPKLSATGRSAGPVSRTIAFGFDLFFVSVLFGMVAYLGRSLVELFTG
                     DIVHPTRGAGIAWTVGYFTWFGLYLWGSIEIAGRTPGKALVGLRVADLEGKPLGPGRA
                     LVRTLAFPFSFILGLGFIPAVTRRDRRALHELVAGSKEIVDWGDREAGIPSALEGWVG
                     RQRDAQVIALTAAAAAASTSDPEVVEIALDPAPEAVEAEPVAEAEAEADEADPVAALS
                     SIPVLVEEVDQGALEPPTDGDGDGA"
BASE COUNT          430 a         1026 c          956 g          387 t
ORIGIN      
        1 ccaccaaccc acccaccaac ccacccacca acccacccac caacccacga acttttcgtg
       61 aagttggggg ctctgccccc acgatcacga aaagttcaga tcggtggggt ggggtggagt
      121 ggagtggggt ggggggcgtc aggagaggag ggcggtgagg atgccaccga cgaggaggcc
      181 ggcgcccatg ccggcggtga cgtcgagtgg gttgtgggcg ccgacgaaga cccggccgac
      241 ggccacgagc gcggccagca ggaacgggat gagggcgaca ccggcgtcga ggtgaccggt
      301 gaggaccgtg ccgacggcgg ccacgaggat gacgtggcct gacgggaagc tcatgccctc
      361 gtgggggacg tcggcgccgc ggaggcgggc gcccggctcg ctcgtgccgg gccgctggcg
      421 cacctccatg tactgcgaca gctcgcggcg cacgacccgt tcgacgacga gcttgagcgc
      481 catggccaag atgacggcga cggcgacctc gatcgcgccg agcacccagg cgatgacgag
      541 gcccaccacg gtcccgacga gcaggttccc gagttgcatc gggagccaca gggggacgta
      601 cagccagtcg ggccagctgt tgacgaagcg gaaggcacgg gcctcggctg gatggatcgt
      661 cggggagcgc gcgccgacga tcgatccgcc caagatcgcg gctccggccg cggcgatggc
      721 caaccccgtc acgtccacag gcgtgaacct acgagatggc agcgcccgcc ccattgccgt
      781 cgcccgcggg ccggcggacg gccggcaccg accgagtgcg cggtggggct tgacctggcg
      841 tgaccgcgtg gtcacactgg cctcggcccc aggtcgtacc cggggtcggc ctcaccccgt
      901 agggtgacgt cggacaatcg ccgcgggaca ggcgagctcg tttctccaca tcgtcccgcg
      961 acgggtccgc ctccgtggac atcccccacg gctcaaggga aacgaaaggc gcaacccatg
     1021 cttgctaccg aatgggggac cgggcaggtc ctcctctcga tgctctactt cacgatgttc
     1081 ttcatctgga tctggctcct gatcatggtg ttctcggaca tcttccggag ccatgacctg
     1141 agcggcgtcg ccaagttcgt ctggatcttc ttcgtgatcc tgctgccgta cctcggggtc
     1201 ttcgtgtacc tcatcgcccg cggccacaag atgggcgagc acgccgttgc cgacgccaag
     1261 gcgcgcgacg aggcggcgca ggcctacatc cgcagcgccg tcggcaccac cagcggtggc
     1321 cacagcacgg ccgccgagct cgagaagctg gctgccctgc gcgacagcgg cgtgctcacc
     1381 gccgacgagt tcgaggccca gaaggccaag ctgctcgccg gctgagctcc cgtctgatga
     1441 cgccgaccgc ggcacggagg cccccgggtc tccgtgccgc gccgcgtccg gggacggacg
     1501 cggcacgccc ggcccggctc cggagccagc gagtacggtc ccgaacgtga ccgagcaccg
     1561 atcacccgtc ggccgtgtca tgggggcgct cgtccccacc gtggtcgatg ccgtcgaccc
     1621 cgatgcgctc atcgaccgcc tcgacatcga tgccctcatg gagcgcgtcg acgtcgacgc
     1681 gctcatcacc cgtgtcgacc tcgacgccct gctgacccgc atcgacctcg acgccctgct
     1741 cggtcggatc gacctcgacg ccctgctggc ccgcatcgac gtgggggcgc tcatcgcccg
     1801 catggacatg aacgaggtca tcggccaggt cgacatgaac gcggtgatcg gccaggtcga
     1861 cctcaacgcc ctgctcgacg acgtcgacat caaggccgtc gtggccaagg ccggcatcga
     1921 cgagatcgtc gccgaggcca gcaccggcgt catcacccgc acgctcgacc tcgcccgccg
     1981 gcagctggtc ggcatcgaca tcatcatcat gggcgccgtc gaccggatct tccgccggca
     2041 gcgccccgcc ctgccggaca cgccgaagct gagcgccacc ggccggtcgg ccggacccgt
     2101 cagccgcacg atcgccttcg gcttcgacct gttcttcgtg tcggtgctgt tcggcatggt
     2161 cgcctacctc ggacgctcgc tcgtcgagct gttcacgggc gacatcgtcc accccacccg
     2221 cggcgcgggc atcgcgtgga ccgtcgggta cttcacctgg ttcgggctct acctctgggg
     2281 cagcatcgag atcgccggcc gcaccccggg caaggcgctg gtggggctgc gcgtagccga
     2341 cctggagggc aagcccctcg ggcccgggcg cgccctcgtg cgcacgctcg ccttcccgtt
     2401 cagcttcatc ctgggcctcg gcttcatccc ggcggtcacc cgcagggacc ggcgggcgct
     2461 gcacgagctg gtcgccggca gcaaggagat cgtcgactgg ggcgaccgcg aggccggcat
     2521 cccctccgcc ctcgaaggct gggtcggtcg ccagcgcgac gcccaggtca tcgccctcac
     2581 cgccgcggcg gcggccgcat ccacgagcga ccccgaggtg gtcgagatcg ccctcgatcc
     2641 ggccccggag gccgtcgaag ccgagccggt ggccgaagcc gaagccgagg ctgacgaggc
     2701 cgatccggtg gccgccctgt cctcgatccc cgtcctcgtc gaagaggtcg accagggagc
     2761 gctcgaaccc ccgaccgacg gcgacggcga cggcgccga
//
LOCUS       DATMFS010000438         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_38869, whole genome shotgun sequence.
ACCESSION   DATMFS010000438 DATMFS010000000
VERSION     DATMFS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38869"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2078)
                     /locus_tag="VNQ33_08320"
     CDS             complement(<1..>2078)
                     /locus_tag="VNQ33_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HWJ98150.1"
                     /translation="GAAAVAVAATVAGTAEPVGSGSGLTASPAETGEAGRGPAAATAT
                     EEVLLLAGADAADLLGQLDAWTPGAATLTVPGPDDGPARLAVVEPNDRRLTLARKVLE
                     RGTPFRGRNDVWFEPGDDTGIRGGKVAFLFPGVEPVFEPRVDDVAEQLEIAWEGLADD
                     VPPLERQGRGIVQVGLLLDEALARIGVRPDLAAGHSLGEWTGQIVSGQIPTTYVDDFL
                     DSLQPGSIEVSDVVFVALGAGIDVAESLVDGLADAYVSHDNCPHQSVICAPAASMAVA
                     VERAKERKVLAQELPFRSGFHSPLFAPFVASMAEQFSGIPLTVADVPMWSATTVAPYP
                     DDPAGITELSGRHLVEPVRFRELADALYADGVRAFVQVGTGSLLGFVDDTLRDEDILT
                     ISANTAKQTGVDQLRRVAAALWSVGHAVDFAPLGHAVATTTPADRTSYASTTESSSSP
                     TKFAASSGGSQPLVLGSTLVRDLTPIDRPVADGPSDADWAELDAPGPLIDEFRGGLAD
                     ASAAALDVLRAARDSKRSLAAPIARTTPPLRTSHVSAPESSFSPTKFDRGEGVVEPDE
                     AGPSLKETVHHLSVAEQPFWLDHAFYEQPAGWPVVDDLFPLVPMTAIIEMLQHAAEDL
                     VPGTVATRVEAIRAFKWLTVHPPTAVTVRATRDGDGPDPATGETTVKASIDKHARATV
                     VVAEGYPEPPA"
BASE COUNT          284 a          715 c          805 g          274 t
ORIGIN      
        1 gccggtggct ccgggtagcc ctccgccacg acgacggtcg cccgggcgtg cttgtcgatc
       61 gacgccttca cggtcgtctc gccggtcgcc gggtccgggc cgtcgccgtc ccgggtcgcg
      121 cgcacggtca ccgccgtggg cgggtggacg gtcagccact tgaacgcccg gatcgcctcg
      181 acccgcgtcg ccacggttcc cggcaccagg tcctcggccg cgtgctggag catctcgatg
      241 atcgccgtca tgggcacgag cgggaacagg tcgtccacca ccggccagcc cgcgggctgc
      301 tcgtagaacg cgtggtccag ccagaacggc tgctcggcga ctgacaggtg gtgcaccgtc
      361 tccttcagcg acgggcccgc ctcgtcgggt tcgaccacgc cttccccccg gtcgaacttc
      421 gtaggtgaga acgacgattc gggcgcgctc acatgcgaag ttcggagggg tggggtggtg
      481 cgggcgatgg gggcggcgag ggagcgcttc gagtcccgtg ccgctcggag gacgtcgagg
      541 gcggcggcgc tggcgtcggc gaggccgccg cggaactcat cgatcagcgg gccgggagca
      601 tcgagctcgg cccagtcggc gtcgctcggg ccgtcggcca cgggtcggtc gatcggggtc
      661 aggtcccgga ccagcgtcga tccgagcacc agcggctggc tcccacccga gcttgcggcg
      721 aacttcgtag gcgaggacga cgattcggtc gtgctggcat acgaagttcg atcggcgggg
      781 gtggtggtgg cgacggcgtg gccgaggggg gcgaagtcga cggcgtggcc gaccgaccag
      841 agggcggcgg cgacgcgccg gagctggtcg acgccggtct gcttcgccgt gttggccgag
      901 atggtgagga tgtcctcgtc gcgcagcgtg tcgtcgacga agccgagcag cgagccggtg
      961 ccgacctgga cgaaggcgcg cacgccgtcg gcgtagaggg cgtcagccag ctcgcggaag
     1021 cggaccggct ccaccaggtg gcggcccgac agctcggtga tgccggccgg gtcgtcgggg
     1081 tacggagcga ccgtggtggc cgaccacatc ggcacgtccg cgacggtgag cgggatgccc
     1141 gagaactgct cggccatcga cgccacgaac ggggcgaaca gcggcgagtg gaagccggag
     1201 cggaacggca gctcctgggc caggaccttg cgctccttgg cccgctcgac ggcgacggcc
     1261 atcgacgcgg cgggggcgca gatcacgctc tggtgcgggc agttgtcgtg ggagacgtag
     1321 gcgtcggcca ggccgtcgac cagcgactcg gccacgtcga tgcccgcacc gagggcgacg
     1381 aagaccacgt cggacacctc gatcgagccg ggttgcaggc tgtcgaggaa gtcgtcgacg
     1441 taggtggtgg ggatctggcc ggacacgatc tggccggtcc actcgccgag gctgtgaccc
     1501 gccgccaggt ccgggcggac gccgatcctc gccagtgcct cgtcgagcag cagaccgacc
     1561 tggacgatgc cgcgaccctg gcgctccagc ggcggcacgt cgtcggccaa gccctcccag
     1621 gcgatctcga gctgctcggc gacgtcgtcc acccgtggct cgaacaccgg ctcgacgccc
     1681 gggaagagga acgccacctt gccgccgcgg atgccggtgt cgtcgcccgg ctcgaaccac
     1741 acgtcgttgc ggccacggaa cggggtcccc cgctccagca ccttgcgggc gagcgtgagg
     1801 cgtcggtcgt tcggctcgac gacggccagc cgagcgggac cgtcgtcggg accaggcacg
     1861 gtgagggtcg ccgcgccagg cgtccaggcg tcgagctggc cgagcaggtc cgcggcatcg
     1921 gccccggcga ggagcagcac ctcctcggtg gcggtcgccg ctgccggccc ccgcccggct
     1981 tcgccggtct cggcgggcga agccgtcagg ccggaacccg agccgacggg ctcggccgtg
     2041 ccggcgacgg tggcggcgac ggcgacggct gccgcacc
//
LOCUS       DATMFS010000439         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_294937, whole genome shotgun sequence.
ACCESSION   DATMFS010000439 DATMFS010000000
VERSION     DATMFS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294937"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1674)
                     /locus_tag="VNQ33_08325"
     CDS             complement(187..1674)
                     /locus_tag="VNQ33_08325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWJ98151.1"
                     /translation="MSERTASETVARSTRADVTAAGAARTPAPRAVSHWRPKALLMAA
                     DALAIVTFTTLAAVLVGRWDLGNLHAAKQLWWTSVLTTPAWLAIFSNQRLYNTRFIGR
                     RIDEFRRVVNASLLGTLTVAVAANVVGILPSRGGLVVLCLSAVVIVTIEREIARRIFL
                     RLRSGGQMTRLVIIAGANPEGRDIAAMLQAEPWLGYEVVGFVDDNAPARQPVPGVPLL
                     GGVAELPGILHEYPHASVIVAASAVESAVTNRLARDLLDQGVHVELSSTLRDISSQRL
                     TVRPLGRFPIVYVEPVTRGGWRAWAKRTFDVAGAGIGLVLAAPILLAVAIAVKIDSKG
                     PVLFRQTRVGRDSEPFSVLKVRTMVIDAEARLAELRELNEADGPLFKMANDPRITRVG
                     RFLRVTSLDELPQLWNVLRGDMSLVGPRPALPHETEEWDALLTQRLRVKPGITGMWQV
                     SGRSDTSFEDYTRLDLYYVDNWSLATDLAILAKTVPVVVLRRGAR"
     gene            1874..2173
                     /locus_tag="VNQ33_08330"
     CDS             1874..2173
                     /locus_tag="VNQ33_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWJ98152.1"
                     /translation="MSLRRLLLPSLALVVGGLLWVASPAGATENPDYTAPPPTTIVTT
                     PVPTTAVKTAVAVTPVRTRLAITGSNATGTAVVGASLVALGAGVLAIRRRRATAL"
     gene            complement(2170..2724)
                     /locus_tag="VNQ33_08335"
     CDS             complement(2170..2724)
                     /locus_tag="VNQ33_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ98153.1"
                     /translation="MSRVLVTVGTDHHRFDRLIGWVDRWAAAHPEAEVLVQHGTADPP
                     ANGTTAVMLGYDELVAEMAAADVVIAQGGPATIMDARSVGQRPIVVPRLARFDEVVDD
                     HQVAFADRMAAKDLVWLAADEDQLHVLIDTALAEPGRVKIPPERGASAATIDAFRAVV
                     DPLTDRRAGRSPWRSRRPAGPAAG"
     gene            complement(2721..3191)
                     /locus_tag="VNQ33_08340"
     CDS             complement(2721..3191)
                     /locus_tag="VNQ33_08340"
                     /inference="COORDINATES: protein motif:HMM:NF020244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98154.1"
                     /translation="MTAARDRPHALLVGSAGGHLAQLVRLGPWTDGCIRTWVTFDKPD
                     ARSLLAGEAVIWAYHPTTRNIPNLVRNSVLAIRHVRRLRPDIVVSTGAAVAVPFFLVA
                     RLLRIPTVYVEVFDRIDSPTLTGRLCRPIATRFCVQWPEQQAHYPGSTVIGPLL"
BASE COUNT          481 a         1118 c         1270 g          428 t
ORIGIN      
        1 gccatgcagc gcatccacca cgaccccgcc acccgcgcct acgtcgaacg acgacgcacc
       61 gacccccgca accccaagac cgaccgcgag atccgccgat gcctcaagcg ctacatcgcc
      121 cgccagctct accgccagct cgaacaaccc cttgacgacc tataggagcg tcggccggag
      181 cgggggctac cgggcgccgc ggcggagcac gacgacgggg acggtcttgg cgaggatcgc
      241 caggtcggtg gcgagcgacc agttgtcgac gtagtacagg tccaggcggg tgtagtcctc
      301 gaacgacgtg tccgagcggc cgctcacctg ccacatgccg gtgatgccgg gcttgacccg
      361 caggcgctgc gtgagcaggg cgtcccactc ctcggtctcg tgggggagtg ccggacgggg
      421 gccgacgagg ctcatgtcgc ctcggagcac gttccacagc tgcggcagct cgtcgagcga
      481 ggtgacgcgc aagaagcgcc cgacgcgggt gatgcgaggg tcgttcgcca tcttgaacag
      541 gggcccgtcg gcctcgttga gctcccggag ctcggccagc cgagcttcgg cgtcgatgac
      601 catcgtgcgg accttcagca cggagaacgg ctccgagtct cgaccgacgc gcgtctggcg
      661 gaacaggacc gggcccttgg agtcgatctt cacggcgatg gcgaccgcga ggaggatggg
      721 cgcggcgagc accagtccga tgccggcgcc ggcgacgtcg aaggtccgct tggcccaggc
      781 gcgccagccg ccccgggtga cgggctccac gtagacgatg gggaaccggc cgagcgggcg
      841 gacggtgagg cgctgcgacg agatgtcacg cagggtggac gacagctcga cgtgcacgcc
      901 ctggtcgagc aggtcgcggg ccaggcggtt ggtgacggcg ctctcgacgg cgctggctgc
      961 cacgatcacg ctggcgtgcg ggtactcgtg gaggatgccc gggagctcgg ccacgccgcc
     1021 gagcagcggg acaccgggca cgggctggcg ggcgggggcg ttgtcatcga cgaagccgac
     1081 gacctcgtag ccgagccagg gctcggcctg gagcatggcg gcgatgtctc ggccctcggg
     1141 gttggccccg gcgatgatca ccaggcgggt catctgcccg ccggagcgga ggcggaggaa
     1201 gatgcggcgg gcgatctccc gctcgatcgt gacgatcacg accgcggaga ggcagaggac
     1261 cacgaggccg ccgcggctgg ggaggatgcc gaccacgttg gcggcgacgg ccacggtcag
     1321 cgtgccgagg agcgaggcgt tgacgactcg gcggaactcg tcgatgcggc ggccgatgaa
     1381 gcgggtgttg tagaggcgct ggttggagaa gatggcgagc caggccgggg tggtgagcac
     1441 cgacgtccac cagagctgct tggcagcgtg gaggttcccc aggtcccacc ggcccacgag
     1501 cacggcggcc agcgtggtga aggtgacgat ggccagcgcg tcggcggcca tgagcagcgc
     1561 cttggggcgc cagtgcgaga cggcgcgggg cgccggagtc cgcgccgcgc ccgcggcggt
     1621 gacgtcagcc cgggtcgacc gggcgaccgt ctcgctggcg gtgcgctcag acatcgtcac
     1681 ggagcgtagc ggatgagggg aacatggtca caacgggtca ccaaggcgcg ggcgggcggt
     1741 cgggtcgaag cgggcgaacg ggcctcctat cggcaccgat ccagcggccg agaggggtgc
     1801 cgggtcccgg tgcccccgag actatctcac gtagcgtgtt gacgagtcgg atatgaacgc
     1861 ttagggtggc cgcatgtcgt tgcgccgcct gctcctgccg tccctcgcac tcgtggtcgg
     1921 agggctgctg tgggtggcgt cgccggctgg tgcgacggag aacccggact acacggcgcc
     1981 gccgcccacc acgatcgtca ccaccccggt gcccaccacc gcggtcaaga cggccgtcgc
     2041 cgtcacgccg gtgcgcaccc gcctggccat caccggcagc aacgccaccg gcaccgccgt
     2101 cgtcggcgcc agcctggtgg ccctgggtgc cggcgtcttg gcgatccgcc ggcggcgggc
     2161 cacggcgctc tagccggctg ctggtccggc cgggcgccgg ctgcgccagg ggctccgccc
     2221 ggcccgacgg tcggtgagcg ggtcgaccac ggcgcggaac gcgtcgatcg tggcggccga
     2281 tgcgccgcgc tcgggcggga tcttcacccg cccgggttcg gccagcgcgg tgtcgatcag
     2341 cacgtgcagc tggtcctcgt cggccgccag ccacacgagg tccttggcgg ccatccgatc
     2401 cgcgaacgcg acctggtggt cgtcgacgac ctcgtcgaag cgagccagcc gcgggaccac
     2461 gatgggccgt tgccccaccg accgggcgtc catgatcgtg gcggggccgc cctgggcgat
     2521 gaccacgtcg gccgcggcca tctcggcgac gagctcgtcg tagccgagca tcaccgccgt
     2581 cgtgccgttg gccggtggat cggccgtgcc gtgctgcacc aggacctcgg cttcggggtg
     2641 ggcggccgcc cagcggtcga cccagccgat gagccggtcg aagcggtggt gatcggtgcc
     2701 gacggtgacc aggacccggc tcacaggagc ggcccgatca cggtgctgcc cgggtagtgc
     2761 gcctgctgct cgggccactg cacgcagaac cgggtggcga tgggccggca cagccgtccg
     2821 gtgagcgtgg gcgagtcgat ccggtcgaac acctccacgt acaccgtcgg gatccgcagg
     2881 aggcgggcca cgaggaagaa cgggacggcg acggcggcgc cggtggacac cacgatgtcg
     2941 ggccgcaggc gccgcacgtg ccggatggcg agcaccgagt tgcggaccag gttggggatg
     3001 ttccgggtgg tggggtggta ggcccagatc acggcctcgc cggccagcag cgaccgggcg
     3061 tcgggcttgt cgaaggtcac ccacgtccgg atgcagccgt cggtccacgg cccgagccgg
     3121 acgagctggg ccaggtggcc gcctgccgac ccgacgagca gcgcgtgggg gcggtcgcgg
     3181 gcggcggtca tcgggcgaac ggtagccgtc ggttggcctg cgcccgagca gggtccgtac
     3241 cctcggcaac gccatgatcg agctcgacgc tgcctccttc ctgctgcagt gggccgt
//
LOCUS       DATMFS010000440         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_268966, whole genome shotgun sequence.
ACCESSION   DATMFS010000440 DATMFS010000000
VERSION     DATMFS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268966"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNQ33_08345"
     CDS             complement(<1..278)
                     /locus_tag="VNQ33_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98155.1"
                     /translation="MSAVEDQIHRELVRTAAGALPDASGIAELVREADPLRPPDAVFR
                     SVAAIQAQLDGLGVLEPLLRDPDVTDVMVNGPGPVWVERRGRLVETPVV"
     gene            complement(275..919)
                     /locus_tag="VNQ33_08350"
     CDS             complement(275..919)
                     /locus_tag="VNQ33_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98156.1"
                     /translation="MLVCWSVGGGAGTSVVVAGLALAAARAEVPALVVDLGGDQPLIF
                     GVPELPGLGLAQWLGASPDVGGDALRRLEVDLAPGVALVPRGDGALGSERTDDLVDAL
                     GGDGRFVVVDAGHVAPGGFVESVVAAAERTLLVARACPMTLRRLERLTTRPAEVVVVR
                     DRRRSVTWRDVAEASGAPVVAELEIDPAVAAAVDAGLDRRPLPRSFLRVLGGVR"
     gene            complement(1279..2217)
                     /locus_tag="VNQ33_08355"
     CDS             complement(1279..2217)
                     /locus_tag="VNQ33_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ98157.1"
                     /translation="MAKERVERDEEDLVRLYLTDIGQYPLLTKDDEVRLAQAIEAGNT
                     AREEMEAGGKELAAARKRELRRLSREGERAERTFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLA
                     RLQKARARLELKLGRPATLSELSAEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAEL
                     GDVVEDRSAESPFEVAATALLPEEISRLLSPLDEREREILKLRFGLDRGEPRTLEEVG
                     EHFNLTRERIRQIEARAMSKLRHPSSDTGARDLLAV"
     gene            2307..>2691
                     /locus_tag="VNQ33_08360"
     CDS             2307..>2691
                     /locus_tag="VNQ33_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98158.1"
                     /translation="MNPGFPYDTDRVKTTKHPDFMNDAVATPASPIVPADWGLLDAEA
                     QHEAFPDTFPIPTSDERASLRAGDMVKLVFVLDPPPASGPNAERMWVEVRTALPDDSY
                     EGWLTNRPKVITSLEPSSVVAFSAKH"
BASE COUNT          423 a          984 c          913 g          371 t
ORIGIN      
        1 accacggggg tctcgaccag ccggccgcgc cgctcgaccc acaccggccc gggcccgttg
       61 accatcacgt cggtcacgtc ggggtcccgc agcagcggct ccaggacgcc cagcccgtcg
      121 agctgcgcct ggatggcggc caccgaccgg aagaccgcat caggcggccg gagcggatcg
      181 gcttctcgca cgagctcggc gatcccggac gcatcgggca gcgcccccgc cgcggttcgc
      241 accagctccc ggtggatctg gtcctccacg gcgctcaccg cacaccgccc aggacccgca
      301 gaaaggaacg aggcaacggc cgccgatcca gtccggcatc gaccgcggcc gcgaccgccg
      361 ggtcgatctc gagctccgcc acgaccggtg caccactggc ctcggcgacg tcgcgccagg
      421 tgaccgaccg ccgacgatcc cggacgacga ccacctctgc ggggcgggtg gtgagccgct
      481 ccaaccgacg gagggtcatc ggacacgccc gggcgacgag gagcgtgcgc tctgccgccg
      541 ccaccaccga ctccacgaac ccgcccggag cgacatgacc ggcgtcgacc accacgaacc
      601 ggccatcgcc gccgagcgcg tcgaccagat cgtccgtccg ttccgacccg agcgcaccgt
      661 cgccccgggg cacgagcgcc acaccgggag cgaggtccac ctcgagccgc cggagcgcat
      721 caccgccgac gtccgggctc gccccgagcc actgagcgag gccgaggccg ggcagctccg
      781 gcaccccgaa gatcagcggc tggtcgccac cgaggtcgac cacgagcgcc ggcacctctg
      841 cccgcgccgc cgcgagggcc aacccggcca ccaccaccga cgtgccggct ccaccgccga
      901 ccgaccaaca cacgagcacc tgcgcacctc cgcgccacga agacctggcg ggaaggcgag
      961 tgcgaaccta cgcggaagcc tcgtgtgcct caacctctga tgagcagcgt tttcgaacct
     1021 gctggaccac cgtctcctgc cggggcgcgc aacagcctgg gagggacctc ccaggctgtt
     1081 gcgcgtttca cgttgcacgg tgacaacacg tcaccgtggg cgacctcaga ccgtgcggac
     1141 cttcttggcg gcaacagcgg cgagagcgac gagggcgatg atgatgagca gcttcttcat
     1201 gagggtgagt gtagggcgcg cgaccccggc gtgcgtccga gacgcctcgg ccgcgacgac
     1261 cgggccctca gcgaaggatc agacggcgag caggtcgcgg gcgccggtgt ccgaagacgg
     1321 gtggcgcagc ttgctcatgg cacgcgcctc gatctggcgg atgcgctccc gggtgaggtt
     1381 gaagtgctcg cccacctctt cgagggttcg aggctcgccg cggtcgaggc cgaagcggag
     1441 cttcaggatc tcccgctcgc gctcgtcgag cggcgagagc agccgggaga tctcctcggg
     1501 cagcagcgcg gtggccgcca cctcgaaggg cgactcggcc gagcggtcct cgaccacgtc
     1561 cccgagctcg gcatcgccgt cctcgcggag cggctcggag agcgacaggg gctcagcggc
     1621 gaagcggagg gcctcggtca ccttgtcctc gggcatctcg acctcggccg acagctcgga
     1681 gagcgtcgcc gggcgaccga gcttgagctc gaggcgggca cgggccttct gcaggcgggc
     1741 gagggtgtcg ccggcgtgca cggggaggcg gatggtgcgg cctgtgttgg cgatgccgcg
     1801 ggtgatcgcc tggcggatcc accacgtggc gtaggtggag aacttgaagc ccttgcgcca
     1861 gtcgaacttc tcgacggcgt gcatcaggcc gaggttgccc tcctggatca ggtccaggag
     1921 cgggaggccc gacgcctggt acttcttggc gatggacacc acgaggcgca ggttcgactg
     1981 cacgaacgtg cgctcggcgc gctcgccctc gcgggagagg cggcgcagct cgcgcttgcg
     2041 cgcggccgcc agctccttgc cgccggcctc catctcttcc cgagcggtgt tgccggcctc
     2101 gatggcctga gcgaggcgga cctcgtcgtc cttggtgagc agtgggtact gtccgatgtc
     2161 ggtgaggtac agccggacga ggtcctcttc gtcccgctca acacgctctt tcgccatctt
     2221 gcaccttttc tgtggaaact gagacccgga tgccccggct gaagtcgaga tcccccaccc
     2281 gagggcggca gggacctagc ctgggcatga atccgggctt cccttacgat accgaccggg
     2341 tcaagaccac caaacatccg gatttcatga acgacgctgt tgccactcct gcgagcccca
     2401 tcgtccctgc tgactggggt cttctcgacg ccgaagccca gcacgaagcg ttccccgaca
     2461 cgttccccat cccgacctcg gacgagcggg cgtcgttgcg tgcaggcgac atggtgaagc
     2521 tcgtgttcgt cctcgatccg ccaccggcct cggggccgaa cgctgagcgg atgtgggtcg
     2581 aggtgcgcac cgccctcccc gacgacagct acgagggctg gctcaccaac cgcccgaagg
     2641 tcatcacgtc gctcgagccg tcgtccgtgg tcgcgttcag cgccaagcac g
//
LOCUS       DATMFS010000441         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_294981, whole genome shotgun sequence.
ACCESSION   DATMFS010000441 DATMFS010000000
VERSION     DATMFS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294981"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VNQ33_08365"
     CDS             complement(<1..242)
                     /locus_tag="VNQ33_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWJ98159.1"
                     /translation="MSRYHLVTLGCPKNEVDSEKLVGQLGADGFAATDDPDQADLLVV
                     NTCAFIGEARQESIDTILALAEHKGDGAKLVVTGCMA"
     gene            complement(296..1378)
                     /locus_tag="VNQ33_08370"
     CDS             complement(296..1378)
                     /locus_tag="VNQ33_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98160.1"
                     /translation="MKWFAIVVVGMALFAGCSSSAEPGERSGEKGAASEEPLVVLQIP
                     VEGAVDDPAVGFDAAWAHGVLPGQPAPETQMAGVPDPRTYAVYRFDEKSGAVTDTIPV
                     GPGGTSLVVGHGAVWMGSTAGRVSRIDPATKQVTGEIPVDGEVWSLSAGEGAIWASGV
                     TDPMRQRDGVVFRIDPKAGTAETVSAGEDHGVVDAVVAGEGWVWAVVNLAPLGGEEVA
                     RVVRIDPSTRQIAGETKVPGTVIGSPVPAKGGAWMLKRGPDGTVSMIRIEGDGTVVEA
                     PAGSITPDRGLGGSILGGMWGITRSPEPGGLIAVRYDPSSGKALEQRKLEGDLSGELL
                     EIEVGARDLWVGTYQSGGGLARVGPL"
     gene            1478..2335
                     /locus_tag="VNQ33_08375"
     CDS             1478..2335
                     /locus_tag="VNQ33_08375"
                     /inference="COORDINATES: protein motif:HMM:NF027609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HWJ98161.1"
                     /translation="MELRIDPLTGVQVQVTASRQQRPNRPTSDCAFCVGGTEAPEPYR
                     VRWFKNRFPSFPDDRCEVVLYAPDHEARLPHLPADHVRAVVDLWADRTAALGGRDDLA
                     YVLVFENNGEAVGATIPHPHGQIYSYPDVPALPATELARLADGHRLLEDDRPELTVIE
                     RDGWRAWVPSAPIYPYGMRVAPIEQRPDLPSLDGADRDAFGSVFTEALRRLEHVFDPP
                     MPYVFWIHQRPFDGSDWPQARLHVEIAGPHRAPGVMRYVAGAELGSGAYLNPIPPEDA
                     AANLRAAAP"
     gene            2332..3342
                     /gene="galK"
                     /locus_tag="VNQ33_08380"
     CDS             2332..3342
                     /gene="galK"
                     /locus_tag="VNQ33_08380"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HWJ98162.1"
                     /translation="MTDGERITAVGPGRVNLIGEHTDSSGGLVLPLAIDLATTVTGTR
                     GGDEVVLVSPDEEHPAVVPLDVADPAAASPGWARYVAGVVAQLRPAEGFVGEVATTLP
                     IGAGLSSSAALEVALALALGFEGSPTDLARITQLAEQQASGVPCGIMDQLASAAGVAG
                     HALLIDCHTLDITPIAVPDDVEIRVIHSGQTRELAGSAYAERTESVAAAEEVLGPLRL
                     LTDPDAADALDDPVLRRRARHVISENERVRAVAAALPAGDLTAVGEALVASHVSLRDD
                     FEVSTPVLDALVDRLNAADGVIGARLTGAGFGGCVVALTEPGALDEGWPVRASAGARL
                     EA"
BASE COUNT          455 a         1369 c         1223 g          474 t
ORIGIN      
        1 gccatgcagc cggtgaccac gagcttggcg ccgtcgccct tgtgctcagc cagggcgagg
       61 atcgtgtcga tcgactcctg gcgggcttcg ccgatgaagg cgcaggtgtt gacgacgagc
      121 aggtcggcct ggtcggggtc gtcggtcgcg gcgaacccgt cggcgcccag ctggccgacg
      181 agcttctccg aatcgacctc gttcttggga cacccgaggg tgaccaggtg gtaccgcgac
      241 atggtccgcc aacgctaccg gccggcctcc ggatcgggag agccgggcgc gctggtcaga
      301 gcggaccgac gcgggcgagc ccgccaccgg actggtaggt gccgacccac aggtcccggg
      361 cgccgacctc gatctcgagc agctcgccgg acaggtcgcc ttcgagcttg cgctgttcga
      421 gcgccttccc cgagctgggg tcgtaccgca cggcgatcag cccgccaggt tccggggacc
      481 gggtgatccc ccacatgccg ccgaggatgc tcccgcccaa gccgcggtcc ggcgtgatcg
      541 aacccgccgg tgcctcgacc accgtgccgt cgccttcgat gcggatcatc gagacggtgc
      601 cgtcgggccc ccgcttcagc atccaggcgc cgcctttggc cggcaccggt gacccgatga
      661 ccgtgccggg aaccttcgtc tcccccgcga tctgccgcgt cgaagggtcg atgcggacca
      721 cacgggcgac ctcctcgcct ccgagcggcg ccaggttgac caccgcccag acccagccct
      781 cgccggcgac gaccgcatcg acgacgccgt ggtcctcgcc agcggacacg gtctcggccg
      841 tgccggcctt cggatcgatc ctgaacacca ccccgtcccg ctggcgcatg gggtcggtca
      901 cgccgctcgc ccagatggcg ccctcgccag cgctgagcga ccagacctcg ccgtcgacgg
      961 ggatctcgcc cgtgacctgc ttcgtggccg ggtcgatgcg ggagacccga ccggcggtcg
     1021 agcccatcca gaccgcgccg tggcccacca cgaggctcgt gccgcctggc cccaccggga
     1081 tcgtgtcggt gaccgctccg gacttctcgt cgaagcggta cacggcgtag gtcctcgggt
     1141 cgggaacgcc cgccatctgc gtctcgggcg cgggctggcc gggcagcacc ccgtgggccc
     1201 acgcggcgtc gaagcccacc gcagggtcgt ccaccgcgcc ctcgaccggg atctggagga
     1261 ccaccagcgg ctcctcggac gccgcgccct tctcgcccga ccgttcgccc ggctcagcgg
     1321 acgacgagca accggcgaac agcgccatcc ccaccaccac gatcgcgaac cacttcaccg
     1381 ctgggcctcc cgtccaccgc gccctcgacg cgctcggggc aacgtagccg aggcggacga
     1441 accagccgac cgaccgggcg cgtaggttgc ggggcacatg gagctgcgga tcgatccact
     1501 caccggggtg caggtgcagg tcaccgcgtc gcgccagcaa cggcccaacc ggccgacgtc
     1561 ggactgcgcg ttctgcgtgg gcgggaccga agcccccgag ccctaccggg tccgctggtt
     1621 caagaaccgg ttcccgtcgt tccccgacga ccgctgcgag gtcgtgctgt acgcaccgga
     1681 ccacgaggcc cggctcccgc accttcccgc cgatcacgtg cgcgccgtcg tcgacctgtg
     1741 ggcggaccgc acggctgcgc tcggcgggcg cgacgatctg gcctacgtgc tggtgttcga
     1801 gaacaacggg gaggccgtgg gcgccaccat cccgcacccg cacggccaga tctacagcta
     1861 ccccgacgtg cccgccctgc ccgccacgga gctggcccgc ctggccgacg gccaccggct
     1921 gctcgaggac gaccgccccg agctcaccgt catcgagcgg gacggctggc gggcctgggt
     1981 gccctccgcg cccatctacc cgtacggcat gcgagtggcg ccgatcgagc agcgaccgga
     2041 tctgccctcg ctcgatggtg cggatcgcga cgccttcggg tccgtgttca ccgaggcgct
     2101 ccgtcgcctg gagcacgtct tcgacccacc gatgccgtac gtgttctgga tccaccagcg
     2161 gcccttcgac ggcagcgact ggccgcaggc gcggctgcac gtggagatcg ccggcccgca
     2221 ccgtgcgccg ggcgtcatgc gctacgtcgc cggtgccgag ctgggcagcg gcgcctacct
     2281 gaaccccatc cctcccgagg atgcggcggc gaacctccgg gcggcagcac cgtgaccgac
     2341 ggggagcgga tcaccgccgt cggtccgggc cgggtgaacc tgatcggcga gcacaccgac
     2401 tccagtggcg ggttggtgct gccgctcgcc atcgacctgg ccaccaccgt caccggcacc
     2461 cggggcggcg acgaggtcgt gctcgtgtcg cccgacgagg agcacccggc tgtcgtcccg
     2521 ctcgacgtgg ccgacccggc ggccgcgtcc cccggctggg cccggtacgt ggccggggtc
     2581 gtcgcccagc tgcgcccggc cgaggggttc gtgggcgagg tcgccaccac cttgcccatc
     2641 ggcgccggcc tgtcatcgag cgccgcgctc gaggtggccc tcgccctggc cctcggcttc
     2701 gagggctcgc ccaccgacct cgcccgcatc acccagctgg ccgagcagca ggcgtcgggc
     2761 gtcccctgcg ggatcatgga ccagctggcg tccgccgccg gggtggccgg ccacgcgctc
     2821 ctcatcgact gccacaccct ggacatcacg ccgatcgcgg tgcccgacga cgtggagatc
     2881 cgggtgatcc actccggcca gacccgggag ctggccgggt cggcctacgc cgagcgcacc
     2941 gagtcggtcg cggccgccga ggaggtgctg gggccgctcc gcctcctcac cgaccccgat
     3001 gcggccgatg ccctcgacga tccggtcctg cgccgtcggg cccggcacgt catctcggag
     3061 aacgagcggg tccgggccgt cgccgccgcc ctcccagccg gcgacctgac ggcggtcggc
     3121 gaggcgctgg tggccagcca cgtgtcgctg cgcgacgact tcgaggtctc gacgcccgtg
     3181 ctcgacgcgc tggtcgaccg cctcaacgca gccgacggcg tcatcggcgc ccgcctcacg
     3241 ggcgcggggt tcggaggttg cgtcgtagcc ctcaccgagc ccggcgccct cgacgagggc
     3301 tggccggtcc gcgcctcggc cggcgcccgc ctcgaggcct gagaccgccc ctcgatcgat
     3361 gttggtgtgc gaaccgacgc actgcgtcgt ttcgcacacc aggttcggtc ggtcccggtc
     3421 cggctcaggg gcgggcgagg ccggcggcga tggcggtgtg gaggcgacgg cgggcggccg
     3481 gggtgcgcac gccccaccag aacaggtcct gcccgtcgac c
//
LOCUS       DATMFS010000442         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_38987, whole genome shotgun sequence.
ACCESSION   DATMFS010000442 DATMFS010000000
VERSION     DATMFS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38987"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VNQ33_08385"
     CDS             complement(<1..970)
                     /locus_tag="VNQ33_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWJ98163.1"
                     /translation="MPVLNPDDFKIADIGLASFGRKEIQLAEHEMPGLMATRAEYADA
                     QPLKGAKIMGSLHMTIQTAVLIETLTALGADVRWVSCNIFSTQDHAAAAIAVGPNGTT
                     DAPSGVPVFAWKGETLEEYWWCTDQALRWPDGSGPNMILDDGGDATMLVHKGREFELA
                     GSVPPATDEDPEEWGVIIDLLTESVAKEPNRWTEVAAGIKGVTEETTTGVHRLYQMFE
                     AGQLQFPAINVNDSVTKSKFDNLYGCRHSLIDGLNRGTDVMIAGKVGVVFGYGDVGKG
                     CAASLKGQGARVIVTEIDPICALQALMEGYEVRTMEQVVETADIFVT"
     gene            1187..1960
                     /locus_tag="VNQ33_08390"
     CDS             1187..1960
                     /locus_tag="VNQ33_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ98164.1"
                     /translation="MSLERLSDRVVIVTGAASGIGQATAVRLAGEGAKVLASDVNRDG
                     LAETLETANEVAEGGGECITHVADISREADAASTVETAVEAWGRLDGIANIAGMLRSG
                     RTHEFSLDSWNQILAVNLTGTFLMCREALPHLMESKGSIVNAASTSSSFGHPWMGAYA
                     ASKGAIWSFTQTLAVEYCKVPVRANCIAPGSVHSGITSNVTFPDDITKEEGKLVRRIM
                     SPTGFGDPQDVASVVALLLSDDGRHITGECIRIDGGTHA"
BASE COUNT          342 a          736 c          759 g          353 t
ORIGIN      
        1 cggtgacgaa gatgtcggcc gtctccacaa cctgctccat ggtgcggact tcgtagccct
       61 ccatgagcgc ctgcagggcg cagatggggt cgatctcggt cacgatgacg cgagcgccct
      121 ggcccttcag ggaggcagca caacccttgc ccacatcgcc gtagccgaag acgacgccga
      181 ccttgccagc gatcatgacg tcggtaccgc ggttgaggcc atcaatcaga gagtggcggc
      241 agccgtagag gttgtcgaac ttcgacttgg tgaccgagtc gttgacgttg atggccggga
      301 actggagctg gccggcctcg aacatctggt agaggcggtg gaccccggtg gtggtctcct
      361 cggtcacgcc cttgatgcca gcggcgacct cggtccagcg gttgggctct ttggccacgc
      421 tctcggtgag caggtcgatg atgacgcccc actcctcggg atcctcatcg gtagcgggcg
      481 gcaccgagcc ggccagctcg aactcccggc ccttgtggac cagcatggtg gcgtcgccgc
      541 cgtcgtcgag gatcatgttc gggccggagc cgtcgggcca gcgcagggcc tggtccgtgc
      601 accaccagta ctcctcgagg gtctcgccct tccaggcgaa caccggcacg ccggacgggg
      661 cgtcggtggt gccgttcggg ccgacggcga tggcagcggc agcgtggtcc tgggtgctga
      721 agatgttgca cgacacccag cgcacgtcgg cgccgagggc ggtgagcgtc tcgatgagca
      781 cggccgtctg gatggtcatg tgcagcgagc ccatgatctt ggcgcccttg aggggctggg
      841 cgtcggcgta ctcggcgcgg gtcgccatca agccgggcat ctcgtgctcg gcgagctgga
      901 tctccttgcg gccgaagctg gccaggccga tgtcggcgat cttgaagtcg tcggggttca
      961 ggacgggcat gtggggtctc ctccacgtgg tggatcggga tcgcggcgcg agcgccaggg
     1021 ctggcgcggc ggatggcgat caggatgtgg tggcggagct ggagcctgct ggctcaccca
     1081 tgacagccct tccgatggcc cggaggccgg gttccaccgt agccgatggg ccatcgtgca
     1141 ttgcgatacg cgatgagaaa ctggaacgtg ttctagtctg cggggcatgt cgctcgaacg
     1201 cctgtcagac cgagtcgtga tcgtcaccgg agcggcctcc gggatcgggc aggcgacggc
     1261 cgtccgcctg gcgggcgagg gcgccaaggt gctggcttcg gacgtcaacc gagacggcct
     1321 cgccgagacc ctcgagaccg ccaacgaggt ggccgagggc ggtggcgagt gcatcaccca
     1381 cgtggccgac atcagccgcg aggccgacgc cgccagcacc gtcgagaccg cggtcgaggc
     1441 ctggggccgc ctcgacggca tcgccaacat cgccgggatg ctgcggtccg gccgcaccca
     1501 cgagttcagc ctcgacagct ggaaccagat cctcgccgtc aacctcaccg gcaccttcct
     1561 catgtgccgc gaggcgctcc cgcacctcat ggagtccaag ggctcgatcg tgaacgcggc
     1621 gtcgacctcg agctcgttcg gccacccgtg gatgggggcc tacgccgcat ccaagggcgc
     1681 catctggtcc ttcacccaga ccctggccgt cgagtactgc aaggtcccgg tccgagccaa
     1741 ctgcatcgca cccggcagcg tccacagcgg catcacctcg aacgtcacct tccccgacga
     1801 catcaccaag gaggagggga agctggtgcg gcgcatcatg tcgcccaccg gcttcggcga
     1861 tccgcaggac gtggcgtcgg tggtggcgct gctgctgtcc gacgacggca ggcacatcac
     1921 cggcgagtgc atccgcatcg acggcggcac gcacgcatga acgccggagg cacgcgctca
     1981 cgacgcccac tgctcacggc ttgttcgatc tcggcttcgc cgagcgaacc gcacgccgtg
     2041 ttgatggttc gcctcgcctg gcggctcggc tcatgagggt cagcatcgcc acggcgtacc
     2101 tgccgcccga ggagctggtg cccatcgcca aggcggccga cgagctcggc taccacggca
     2161 tggccctggc cgatcacgtc ctcaacctcg
//
LOCUS       DATMFS010000443         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_15231, whole genome shotgun sequence.
ACCESSION   DATMFS010000443 DATMFS010000000
VERSION     DATMFS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15231"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VNQ33_08395"
     CDS             complement(<1..487)
                     /locus_tag="VNQ33_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWJ98165.1"
                     /translation="MADLAPSSDPVEIDQVIIRFAGDSGDGMQLTGDRFTSASAVFGN
                     DLSTLPEFPAEIRAPAGTLAGVSAFQVHISDHDITTPGDAPAVLVAMNPAALRSELHH
                     LVDGGTVIVNTDAFEERNLTKAGYRSDPLTDGTLDRYTVYEVPMTSLTKDAVAPLGVK
                     PR"
     gene            621..1190
                     /locus_tag="VNQ33_08400"
     CDS             621..1190
                     /locus_tag="VNQ33_08400"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWJ98166.1"
                     /translation="MSVRMGILALLAEQPSHGYQLKAAFEERTGGVWSLNVGQVYTTL
                     DRLKADGLVEPMEVESSEGDGRDRRPWRITRDGKEALEAWFGEPAVEPVPAREDFLVK
                     VLMAFHSSPLEALAVIDRQRTELYRLLQDARRTTRRAQAGGGTADPAADLAAELMAEV
                     VASRREADLRWLDICESHLKSQTKKRNPA"
     gene            1187..1942
                     /locus_tag="VNQ33_08405"
     CDS             1187..1942
                     /locus_tag="VNQ33_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98167.1"
                     /translation="MNEPVLQLHGATKTYRHGRVDVAALQGIDLEVDAGSLTAVMGPS
                     GSGKSTLLHLAAGMVGATSGLVRVAGMDLAGRSPAELASLRRHTVGVVFQQYNLVPSL
                     TALENVTLPLELEGGSRKDVHALGRAALERVGVDEPYSRYPDDLSGGQQQRVAIARAV
                     AVPRKVILADEPTGALDTVTGDLILDLLRELADDGAAVLIVTHEPRVASYADRVVTIR
                     DGKRAADTVVPVAPDLSELAVRHAAAVGSEVGA"
     gene            1939..>2390
                     /locus_tag="VNQ33_08410"
     CDS             1939..>2390
                     /locus_tag="VNQ33_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98168.1"
                     /translation="MTSRRGLVVVAARLARREVRRHPWRHLLVTVLIFLPVVTALAAF
                     STVASWHDLEQRRHQFTDPVDGGLEYCCLSYDAKGHVIDLGIDESIDPARTEVWHRTG
                     DWLITDMARPDGQGPRLAGMEVTEAAAGSAASPRFLVEHGRLPRTGKE"
BASE COUNT          340 a          885 c          825 g          340 t
ORIGIN      
        1 cccgtggctt cacccccagc ggggcgacgg cgtccttggt gagcgacgtc atcggcacct
       61 cgtagaccgt gtagcggtcg agcgtgccgt cggtcagcgg gtcagaccgg tagccggcct
      121 tcgtgaggtt ccgctcctcg aacgcgtcgg tgttcacgat gacggtgccg ccgtcgacca
      181 ggtggtgcag ctccgagcgc agcgccgccg ggttcatcgc caccaagacc gcgggcgcat
      241 cgccgggcgt ggtgatgtcg tggtcggaga tgtggacctg gaacgcggag acgccggcga
      301 gggtccccgc gggagcccgg atctcggcgg ggaactccgg cagcgtcgac aggtcgttgc
      361 cgaacacggc gctggccgac gtgaagcggt cgcccgtgag ctgcatgcca tcgccggaat
      421 cgccggcgaa gcggatgatg acctggtcga tctcgaccgg gtccgatgac ggtgcaaggt
      481 cggccacgtt gcctcctcgg tgtcccctgc ggggaatccc cttcccccgc ttcgcagggt
      541 atccacgccg ccaccgcttc gcggcggacc ccggcccgga tccttgaccg atgcattctg
      601 agtatgcata ctcaggaacc atgagcgtcc ggatgggcat cctcgccctg ctggctgagc
      661 agccgtcgca cggctaccag ctcaaggccg cgttcgagga gcggacgggc ggcgtgtggt
      721 cgctcaacgt cggccaggtg tacacgaccc tcgaccgcct caaggccgat gggctcgtcg
      781 aaccgatgga ggtcgagagc agcgagggcg acgggcggga ccggcggccg tggcgcatca
      841 cccgcgatgg caaggaggcg ctggaggcct ggttcggcga gccggccgtg gagccggtcc
      901 ccgcccgcga ggacttcctg gtcaaggtgc tgatggcgtt ccacagctcc ccgctggagg
      961 ccctcgccgt catcgaccgc cagcgcaccg agctctaccg gctgctccag gacgcccggc
     1021 gcaccacccg gcgggcccag gccgggggcg gcaccgccga ccctgccgcc gacctcgccg
     1081 ccgagctcat ggccgaggtc gtggcctccc gtcgagaagc cgacctccgc tggctcgaca
     1141 tctgcgaatc gcacctcaaa tcccagacca agaaacggaa cccggcgtga acgaacccgt
     1201 cctccagctc cacggagcga ccaagaccta ccggcacggc cgcgtcgacg tcgccgccct
     1261 gcagggcatc gacctcgagg tcgatgccgg ctccctcacc gcggtcatgg gccccagcgg
     1321 atcggggaag tcgaccctcc tgcacctcgc cgccggcatg gtcggcgcca cctccggcct
     1381 ggtgcgcgtg gccggcatgg acctcgccgg ccgctccccc gccgagctgg cctcgctgcg
     1441 ccggcacacc gtgggcgtcg tcttccagca gtacaacctg gtgccctcgc tcaccgcgct
     1501 ggagaacgtc accctgccgc tcgagctcga gggcgggtcg cggaaggacg tccacgcgct
     1561 ggggcgcgcc gcgctcgagc gggtcggtgt cgacgagccg tactcgcggt accccgacga
     1621 cctgtccggc ggccagcagc agcgggtggc catcgcacgg gccgtcgccg tgccccggaa
     1681 ggtgatcctc gccgacgagc cgaccggggc cctcgacacg gtcacgggcg acttgatctt
     1741 ggatctgctc cgcgagctcg ccgacgatgg cgctgcggtg ctgatcgtga cccacgagcc
     1801 gcgggtcgcc agctacgccg accgggtcgt cacgatccgc gacggcaagc gcgccgccga
     1861 caccgtggtc cccgtcgctc ccgacctgtc cgaactggcc gtccgccacg ccgcagccgt
     1921 cggctcggag gtcggcgcat gacctcgcga cggggcttgg tcgtcgtcgc ggcgcggctc
     1981 gcccgacgcg aggtccggcg ccatccctgg cgccacctcc tggtcaccgt gctcatcttc
     2041 cttccggtcg tgaccgcact ggccgccttc tcgaccgttg cgtcgtggca cgacctcgag
     2101 cagcggcgcc atcagttcac cgatcccgtc gacggcggcc tcgagtactg ctgcctcagc
     2161 tacgacgcca agggccacgt gatcgacttg ggcatcgacg agagcatcga cccagctcgc
     2221 accgaggtgt ggcaccgcac cggcgactgg ctgatcaccg acatggcccg acccgacggg
     2281 cagggccccc ggctcgcggg catggaggtc accgaggcag ctgcggggtc ggcggcctcg
     2341 ccccgcttcc tcgtggaaca cgggcgactg ccgaggaccg gcaaggagat
//
LOCUS       DATMFS010000444         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_224339, whole genome shotgun sequence.
ACCESSION   DATMFS010000444 DATMFS010000000
VERSION     DATMFS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224339"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2037)
                     /locus_tag="VNQ33_08415"
     CDS             complement(<1..>2037)
                     /locus_tag="VNQ33_08415"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWJ98169.1"
                     /translation="ELGISVVGGCCGTTPEHLRQVVEAVRDLEVAPRSPVVEPSVTSI
                     YSPVPLEQELSFLIIGERTNANGSKKFREAMLEGDYDTCSAMASEQLKSGAHVLDVCV
                     DYVGRDGTGDMDEIASRFATGANAPLVLDSTEPEVMEAGLQWMGGRAILNSSNLEDGF
                     AEGSRLDRVFRLAKEYGAAVICLCIDEEGQARTAEWKVRVAKRIAALARDTYGLENED
                     LIFDALTFPLSTGDDDLRRDAIETMDAIKAIKEEIPGCFTTLGLSNVSFGLSPASRHV
                     LNSVFMHECQQVGLDSAIVHASKILPLSKIPDEQRDVALDLIYDRRGAAGALSEGAED
                     YDPLAKFLDVFADVQVQKEEKVDRSGWPVGERLHHRIIDGDRENLTDDLDEAMAEGIA
                     PLDIINEHLLGGMKVVGELFGSGEMQLPFVLQSAETMKASVAHLEPYMEKAVDGVDSS
                     KGRIVLATVKGDVHDIGKNLVDIILTNNGYEVHNIGIKISISEMIEKATEVDAHAIGM
                     SGLLVKSTLIMRDNLEELNARELSDIPVLLGGAALTRSYVERDLRGVYRGRLFYGKDA
                     FEGLRVMDQLGEIRRDPSKDDPDWGVVPSESTVRARSGIDKRDTSGIDLPDRSPAVED
                     DNPVFVPPFTGNRIIKGIPLDDIAAYINETALFRNQWGFRPEGGETDDEFKARLR"
BASE COUNT          283 a          646 c          749 g          359 t
ORIGIN      
        1 cgcaggcgcg ccttgaactc gtcgtcggtc tcgccgcctt cggggcggaa gccccactgg
       61 ttgcggaaga gcgccgtctc gttgatgtag gcggcgatgt cgtcgagcgg gatgcccttg
      121 atgatccggt tgccggtgaa cggcggcacg aagaccgggt tgtcgtcttc gaccgccggg
      181 gagcggtcgg gcaggtcgat gcccgaggtg tcgcgcttgt cgatgccgct gcgggcccgg
      241 acggtggact cggagggtac gaccccccag tccgggtcgt ccttcgacgg gtctcggcgg
      301 atctcgccga gctggtccat cacgcggagc ccctcgaagg cgtccttccc gtagaagagg
      361 cggccccggt acacgccgcg cagatcccgc tcgacgtagc tgcgcgtgag ggcggcacca
      421 ccgaggagga ccgggatgtc gctcagctcg cgggcgttga gctcctcgag gttgtcgcgc
      481 atgatcagcg tgctcttcac caggaggccg ctcatgccga tggcgtgggc gtcgacctcg
      541 gtggccttct cgatcatctc ggagatcgag atcttgatcc cgatgttgtg gacctcgtag
      601 ccgttgttgg tgaggatgat gtcgacgagg ttcttgccga tgtcgtggac gtcgcccttc
      661 accgtggcca gcacgatgcg gcccttggag gagtcgacgc cgtcgacggc cttctccatg
      721 tacggctcga ggtgggcgac cgaggccttc atcgtctcgg cgctctgcag cacgaacggg
      781 agctgcatct caccggagcc gaagagctcg ccgacgacct tcatgccgcc gaggaggtgc
      841 tcgttgatga tgtcgagcgg tgcgatgccc tcggccatgg cctcgtcgag gtcgtcggtg
      901 aggttctcgc ggtcgccgtc gatgatgcgg tggtggaggc gctcgccgac gggccacccg
      961 gaccggtcga ccttctcctc cttctggacc tggacgtcgg cgaagacgtc gaggaacttg
     1021 gcgagggggt cgtagtcctc ggcgccctcg gacagcgcac cagccgcacc ccggcggtcg
     1081 tagatgaggt cgagggcgac gtcgcgctgc tcgtcgggga tcttgctcag cgggaggatc
     1141 ttcgaggcgt gcacgatggc ggagtcgagg ccgacctgct ggcactcgtg catgaacacg
     1201 ctgttgagca cgtggcgcga ggcgggggag agaccgaagc tcacgttgga gaggccgagc
     1261 gtggtgaagc agcccgggat ctcctccttg atggccttga tggcgtccat cgtctcgatg
     1321 gcgtcgcggc ggaggtcgtc gtcgccggtc gagagcggga acgtgagggc gtcgaagatc
     1381 aggtcctcgt tctccagccc gtaggtgtcg cgggcgaggg cggcgatgcg cttggcgacc
     1441 cgcaccttcc actcggcggt gcgggcctgg ccctcctcgt cgatgcagag gcagatcacg
     1501 gccgcgccgt actccttggc caaccggaac acgcggtcca ggcgggaccc ctcggcgaag
     1561 ccgtcctcca ggttcgagga gttgaggatg gcccggccgc ccatccactg caggccggcc
     1621 tccatgacct cgggctcggt ggagtccagc accaggggcg cgttggcgcc ggtggcgaag
     1681 cggctggcga tctcgtccat gtcgccggtg ccgtcgcggc cgacgtagtc gacgcagacg
     1741 tcgagcacgt gggcgccgct cttgagctgc tcgctggcca tggccgagca cgtgtcgtag
     1801 tcgccctcga gcatggcctc gcggaacttc ttggagccgt tggcgttggt gcgctcgccg
     1861 atgatgagga agctcagctc ctgctcgagc ggcaccgggc tgtagatcga cgtcacgctc
     1921 ggctcgacga ccggcgagcg gggcgcgacc tcgaggtcgc ggacggcctc gacgacctgg
     1981 cggaggtgct cgggcgtcgt gccgcagcac ccaccgacga cgctgatgcc gagctcg
//
LOCUS       DATMFS010000445         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_85618, whole genome shotgun sequence.
ACCESSION   DATMFS010000445 DATMFS010000000
VERSION     DATMFS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85618"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1445)
                     /locus_tag="VNQ33_08420"
     CDS             complement(213..1445)
                     /locus_tag="VNQ33_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98170.1"
                     /translation="MADFSLALNEDQQQLKDWLHQFSKDVIRPAAEEWDEKEEFPWPI
                     VQEAAKIGLYSVDFVTNAMLGDPTGLTMPIAIEEVFWGDAGIGLSIFGSGLAAAGIAG
                     NGTPEQMIEWVPQCYGTPDDVKLGAFCVSEPDAGSDVSSLRTRAVYDEANDEWVLNGT
                     KAWITNGGIADVHVVVAAVDPELRGRGQASFIVPPNTPGISQGQKYKKHGIKASHTAE
                     VVLEDVRVPGHCLLGGKDKLDEKLARAREALTKPKKESSGKQPAMATFEATRPAVGAQ
                     AIGVARAAYEIALQYAQERNAFGKAIIHNQAIAFMLADMATEIDAARLLVHRAAWLSR
                     NGTYVNAEGSMSKLKAGRVAVWATERAIQILGGYGYTREYGVERMHRDAKIFDIYEGT
                     EQIQQLVISRAISGLRIE"
     gene            complement(1764..3197)
                     /gene="cysS"
                     /locus_tag="VNQ33_08425"
     CDS             complement(1764..3197)
                     /gene="cysS"
                     /locus_tag="VNQ33_08425"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWJ98171.1"
                     /translation="MSVPAIHLYDTRRRAVVPFEPVTPGKALIYTCGPTVYGPQHLGN
                     MRSQLLPDLLRRVLRAAGYEVTFVTNVTDVGHLVSDADEGEDKVEKAAAQTGQSVEEI
                     TGHWTEQWANDRRRLGMIEPDLVPYASKHIPEQIAMIQTLEAQGRTYVIDDGVYFDTS
                     TYPEYADFAHLNLDDLEATGRVENVSDKRHPADFALWKLSPPDQTRLQEWDSPWGVGF
                     PGWHIECSAMATKYLGRHFDIHTGGIDHVRVHHTNEVAQSECALEQHPWVNIWMHNEF
                     LDVGNEKMSKSKGHVLTVDTLVEEGIDPLAFRYFFFQAHYRQQQNFTIDLVRSAGTAL
                     RRIVHHAVAARDAVGGDASGADAARIEPLRAAFWEALADDLNGPKALAVVASVARDAE
                     LADADRWCLLADFDRVLGFGLAEATDPEAADDSADDPRIAALLAERTAARVSKDWAEA
                     DRIRDVLAAEGLEIVDTPDGPQARRRS"
BASE COUNT          442 a         1082 c         1205 g          535 t
ORIGIN      
        1 agcccgtcga gccacccggc cctcaggctc gccggacagc tgatcctgtg ctggtttcgc
       61 tcctcgccag aggtgtccgg cgacagaatg ccgcccgcgt cggcggcacc gtcggcaacg
      121 atctcgatct caccgcggtc gacggccccc gctcgggagc gggggccgtc ggccggtctg
      181 gttcgacgca gccggcccga ggggccgggc cgctactcga tgcggaggcc ggagatggcg
      241 cggctgatga ccagctgctg gatctgctcg gtgccctcgt agatgtcgaa gatcttggcg
      301 tcgcggtgca tgcgctccac gccgtactcc cgggtgtagc cgtagccgcc caggatctgg
      361 atggcccgct cggtggccca cacggcgacg cggccggcct tgagcttgga catcgagccc
      421 tcggcgttga cgtaggtgcc gttgcggctc agccacgcgg cacggtgcac caggaggcgg
      481 gcggcgtcga tctcggtcgc catgtcggcg agcatgaagg cgatggcctg gttgtggatg
      541 atggccttgc cgaacgcgtt gcgctcctgg gcgtactgca gggcgatctc gtaggcggcg
      601 cgggcgacgc cgatggcctg ggcgcccacg gccgggcggg tggcctcgaa cgtggccatg
      661 gccggctgct tgcccgagga ctccttcttc ggcttcgtca gcgcctcacg ggcccgggcg
      721 agcttctcgt cgagcttgtc cttgccgccg aggaggcagt ggccggggac ccggacgtcc
      781 tcgaggacga cctcagcggt gtggctggcc ttgatgccgt gcttcttgta cttctggccc
      841 tgcgagatgc caggggtgtt cggcgggacg atgaagctgg cctggccgcg gccgcgcagc
      901 tcggggtcga cggcggcgac gaccacgtga acatcggcga tgccgccgtt ggtgatccac
      961 gccttggtgc cgttgagcac ccactcgtcg ttggcctcgt cgtagacggc ccgggtgcgc
     1021 agcgagctca cgtcggagcc ggcgtcgggc tcggacacgc agaaggcgcc cagcttcacg
     1081 tcgtcgggcg tgccgtagca ctgcgggacc cactcgatca tctgctcggg cgtgccgttg
     1141 ccggcgatgc ccgcagcggc gaggcccgaa ccgaagatgg agaggccgat gccggcgtcg
     1201 ccccagaaga cctcttcgat ggcgatcggc atggtgaggc cggtggggtc gccgagcatg
     1261 gcgttggtga cgaagtcgac cgagtagagc ccgatcttgg cggcctcctg cacgatcggc
     1321 cacgggaact cctccttctc gtcccactcc tcggcggcgg ggcggatcac gtccttcgag
     1381 aactggtgga gccagtcctt cagctgctgc tgatcctcgt tgagcgcgag cgagaaatct
     1441 gccatgtcgg cacgaccttc cggatgctgg tccaccctgc cggcatcagg cgaggggcgg
     1501 atcacgacgg ttgttaccgg gcggtaacta cggccaggct agaccggcca cggagagcga
     1561 tgccaggcca cgcaacggct cggcgccggt gaacccgccg ccccaggtca cgaacaccct
     1621 cgcgccgccc tggtcctcca acttggtgga tcgtggtcgc atacggcccg catcctccaa
     1681 gttggaaggt gagggcgaaa cgtggaggga gttggacgct aggcgaccac gatcctccaa
     1741 gttccggcgt cggctcggga gcgtcaggag cgccggcggg cttggggacc gtcgggcgtg
     1801 tcgacgatct cgaggccctc ggcggccagg acgtcgcgga tgcggtcggc ctcggcccag
     1861 tccttgctca cccgggccgc ggtccgttcg gccagcaggg cggcgatgcg ggggtcgtcg
     1921 gccgagtcgt cggcggcctc ggggtcggtc gcctcggcca gcccgaaccc gagcacccgg
     1981 tcgaagtcgg cgagcaggca ccaccggtcc gcatcggcca gctcggcatc gcgggcgacg
     2041 gaggcgacga cggccagcgc cttcgggccg ttgaggtcgt cggccagcgc ctcccagaac
     2101 gcggcccgca gcggttcgat gcgggcggcg tcggcgccgg acgcatctcc acccaccgca
     2161 tcacgggcgg cgacggcgtg gtggacgatg cggcgcagcg cggtgccggc cgagcggacg
     2221 aggtcgatgg tgaagttctg ctgctggcgg tagtgcgcct ggaagaagaa gtaccggaac
     2281 gccagggggt cgatgccctc ctccaccagg gtgtcgacgg tgagcacgtg gcccttgctc
     2341 ttcgacatct tctcgttgcc gacgtcgagg aactcgttgt gcatccagat gttcacccac
     2401 gggtgctgct cgagggcgca ctcgctctgg gccacctcgt tggtgtggtg gacccggacg
     2461 tggtcgatgc cgccggtgtg gatgtcgaag tggcggccga ggtacttggt ggccatcgcc
     2521 gagcactcga tgtgccagcc ggggaagccg acgccccacg gcgagtccca ctcctggagc
     2581 cgggtctggt ccggcgggct cagcttccag agggcaaagt cggccgggtg gcgcttgtcg
     2641 gacacgttct cgacccggcc ggtggcctcg aggtcgtcga ggttgaggtg ggcgaagtcg
     2701 gcgtactcgg ggtaggtcga cgtgtcgaag tagacgccgt cgtcgatcac gtaggtgcgg
     2761 ccctgcgctt cgagcgtctg gatcatggcg atctgctcgg gaatgtgctt cgaggcgtac
     2821 gggacgagat cgggctcgat catcccgagg cggcggcggt cgttcgccca ctgctcggtc
     2881 cagtggccgg tgatctcctc gacggactgc ccggtctggg ccgccgcctt ctcgaccttg
     2941 tcctcgccct cgtcggcgtc gctcaccagg tggcccacgt cggtgacgtt ggtgacgaag
     3001 gtgacctcgt agccggcggc ccgcagcacc cgccggagca ggtcgggcag cagctggctg
     3061 cgcatgttgc cgaggtgctg gggcccgtag accgtgggcc cgcacgtgta gatgagcgcc
     3121 ttgccggggg tgacgggctc gaacggcacc acggcgcgcc gccgcgtgtc gtacaggtgg
     3181 atggccggaa cgctcaccgc acgattctgg ccgatcgccc tcccccgccc cgacccgagc
     3241 cgccgccgaa ccgaacgttc gacg
//
LOCUS       DATMFS010000446         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_131972, whole genome shotgun sequence.
ACCESSION   DATMFS010000446 DATMFS010000000
VERSION     DATMFS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131972"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>2197
                     /locus_tag="VNQ33_08430"
     CDS             71..>2197
                     /locus_tag="VNQ33_08430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ98172.1"
                     /translation="MAALAFAAYLATAVWAREISLEGPALIWFPPAAVAIAACFFRPR
                     LVWVIVLAELVSTSAVMGQGEEYGPVLLVVNSAGLALAYAFGAWVLRRLALDPRLRDP
                     EDLAVLALGIGTGAAAATLIGVLVQRAGDVIAADELARSAALFWVGDIVGAACLLPAL
                     VIAGNDLVHHRRLRLADDEDRLPRWQLYAELLAPAVAGLALMEIGEAPMRFVYLAFLP
                     VVIVAVRHGVAAAAVSSAALSAVMTAGAHVQLGDALDRSDFQLLLLVLVATGVTTGSV
                     VSSRRDVSAGKARVSEIIEATPDLVASASRDGTIRYLNPVGRQLLGFSPDADPSSRAF
                     DFFPDDLARDLVREGMRTAERTGTWSGENRLRRPDGHEIPISQVLVAHPDLDDDGQRL
                     YSTICRDMTSQRALEDQLRRAVLYDEATGLPNRVLLVEQLARAVQALDAGRRVAVLFA
                     DLDHLQRVNETFGFAAGEQVVTTIAERLADLVRAQDIVARYGGSQFVLVLADVPDEFE
                     AVLLADRLLTSFAEPVDADGHELQVSGSVGITLAEAGQDHLESLRSAEIALHRAKEAG
                     GGRYALFDDDLQRRSLRRLEREADLHDVLTKQTWTLAYQPIFDSRARVVVGVEALLRW
                     THPTRGPVPPFDLITLAEHTGAIVSLGREIFRRACHEATQWHAMGFDVPVSINVSARQ
                     LREPSFLADVESVLHETGIATDRVVVE"
BASE COUNT          270 a          843 c          762 g          322 t
ORIGIN      
        1 agggctggaa ggggtgcccg gcatggatcc agcacaaggt gcgagcgggt cgcaccgaca
       61 cctcgcgttc gtggcagcac ttgcgttcgc cgcgtacctg gccacggccg tctgggcgcg
      121 cgagatcagc ctcgagggcc cggcgctcat ctggttcccg ccagccgccg tcgccatcgc
      181 ggcgtgcttc ttccgcccgc gcctcgtctg ggtcatcgtc ctggctgaac tggtcagcac
      241 ctccgccgtg atgggccagg gcgaggagta cgggcccgtc ctcctcgtcg tcaactccgc
      301 gggcctcgcc ctcgcgtacg ccttcggtgc ctgggtgctg cggcggctgg ccctcgaccc
      361 gcgcctgcgc gaccccgaag acctggccgt gctggcgctg ggcatcggca ccggcgccgc
      421 cgccgcgacg ctgatcggcg tgctggtgca gcgcgccggc gacgtgatcg ctgccgacga
      481 gctggcccgc agcgcggcgc tgttctgggt gggcgacatc gtcggcgccg cgtgcctgct
      541 cccggccctg gtgatcgccg gcaacgacct ggtgcaccac cggcggctgc ggctcgccga
      601 cgacgaggac cggctccccc gctggcagct ctacgccgag ctcctcgctc ctgccgtcgc
      661 cggactggcg ctgatggaga tcggcgaggc gccgatgcgg ttcgtgtacc tcgccttcct
      721 ccccgtggtc atcgtcgccg tgcgccacgg cgtggcggcg gcggccgtgt cgtccgcagc
      781 gctgtcggcg gtcatgaccg ccggtgccca cgtccagctc ggcgatgcgc tcgaccgctc
      841 cgacttccag ctcctgctgc tcgtgctcgt cgccaccggc gtcacgacgg gttcggtcgt
      901 gagctcacgg cgcgacgtga gcgcggggaa ggcgcgcgtc agcgagatca tcgaggcgac
      961 gcccgacctc gtcgcgtcgg cgtcgcgcga cggcaccatc cgctacctga accccgtggg
     1021 ccggcagctg ctcggcttct cccccgatgc cgacccgtcc agccgggcct tcgacttctt
     1081 ccccgacgac ttggcccgcg acctggtgcg cgagggcatg cgcaccgccg agcggacggg
     1141 gacctggagc ggagagaacc gcctccgccg tcccgacggg cacgagatcc cgatctcgca
     1201 ggtgctggtg gcgcaccccg acctcgacga cgacgggcag cgcctgtaca gcaccatctg
     1261 ccgcgacatg acgagccagc gcgccctcga ggaccagctc cggcgcgccg tgctctacga
     1321 cgaggccacg gggctgccga accgggtgct gctggtggag cagctggccc gtgcggtgca
     1381 ggccctcgac gccggccggc gggtggccgt cttgttcgcc gacctcgatc acctccagcg
     1441 ggtcaacgag acgttcgggt tcgccgccgg cgaacaggtg gtcaccacga tcgccgagcg
     1501 cctggcggac ctcgtccgcg cccaggacat cgtcgcccgc tacggcggct cccagttcgt
     1561 gctggtgctc gccgacgtgc ccgacgagtt cgaggccgtc ctcctcgccg accgcctcct
     1621 cacgtcgttc gccgaaccgg tcgacgccga cggccacgag ctccaggtga gcggcagcgt
     1681 cgggatcacc ctggccgaag ccgggcagga ccacctcgag tcgctgcgca gcgccgagat
     1741 cgccctgcac cgggccaagg aggcgggcgg cgggcgctac gccctgttcg acgacgacct
     1801 ccagcgccgc tccctccgcc gcctcgagcg ggaagccgac ctccacgacg tgctcaccaa
     1861 gcagacctgg acgctcgcct accagccgat cttcgactcg cgagcgcgcg tggtggtcgg
     1921 tgtcgaggcg ctgctccggt ggacccaccc cacccgcggc cccgtccccc cgttcgacct
     1981 gatcacgctc gccgagcaca ccggggcgat cgtgtcgctg gggcgggaga tcttccgccg
     2041 ggcctgccac gaggccaccc agtggcacgc catgggcttc gacgtgccgg tgagcatcaa
     2101 cgtcagcgcc cgccagctgc gcgagccgag cttcctcgcc gacgtggagt cggtgctgca
     2161 cgagacgggc atcgccacgg accgggtggt cgtcgag
//
LOCUS       DATMFS010000447         4143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_318635, whole genome shotgun sequence.
ACCESSION   DATMFS010000447 DATMFS010000000
VERSION     DATMFS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318635"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VNQ33_08435"
     CDS             complement(<1..1317)
                     /locus_tag="VNQ33_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWJ98173.1"
                     /translation="MESAVQDQVMTGAIERVSAGLDELAVALEGWVPADADDAARGIR
                     ETEALGRRLHAMQVGVLSAVDRSGAHRADGHASAKVMVRHCGNLSNAEALRRSQVASA
                     LRDLPVVSAAHAAGRLGVAQAERIARVHANKRVRESMVAEDAAVMKAAVMLPYRDFDE
                     AITDWERLADEDGAGDRAERADANRDFRMPRNLDGSGRFDGGGGGVAMQQIEDVWQRQ
                     VKREWEADWADARCRLGDAATKDDLARTDAQRRFDAMKACFLRGDLAEYGVEARTCTE
                     LVVDDVTFERRLAMLFGTDPGPDPRLATWWDDLTTTLVDLDPAVSSSASPSPAGRLPA
                     ADTEPVPHAGVDPIPPADADSGVDAGVEGDGNVGADANRGGAPRPGTPGGLIGYRCGT
                     VDGRPLDPTEVVVAALIGQVRRTVAGADGVVMDMSRRTRLFTGARQL"
     gene            complement(1741..2601)
                     /locus_tag="VNQ33_08440"
     CDS             complement(1741..2601)
                     /locus_tag="VNQ33_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98174.1"
                     /translation="MIEQAPARPDVRRPSVIQTGLWAVVTLLACATCAIAALVALGVL
                     IEPQSATIDPARLPQGTPWYTVEGVHGNVTLTECPALLGRTEASPAWCEDTDATSVRA
                     LIVAPLVALLTGACTFLAGRRTWMCVRLRSAPPPPWPPNAYRQEGMPWAFWLGLVLVG
                     ALALLIDWVTIASLLDGDGGAGPKGAVFWTAVVALIVTRYGLRSAVSVRIEGGSLEWR
                     APLRTRRIPLAAVVAYEIGGSSVLGERPATLDLADGATLEVSVPNRRHADVLAGFLWS
                     LGIPQRTGTD"
     gene            complement(2723..3382)
                     /locus_tag="VNQ33_08445"
     CDS             complement(2723..3382)
                     /locus_tag="VNQ33_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98175.1"
                     /translation="MTDQKLCDKTAAELQLARDTAMTYPTAQDAKDAGYRLVTGYVPG
                     IAAHWIKGSLIDDTFEIDKPEMILYDGNGLDAHVVGLSYYQWHAGDNKPTQGFTGDND
                     HAHRHIGLCSSTTTGQIIGDSTTTVEDCEARGGSKDDGSKGWMSHAWVVEGCESPWGV
                     FSAASPLLDDQLGASSGTDGGHCAGSGVRDRYGMDDPPSKKRAEPTGEAAKTTGDTTG
                     D"
     gene            complement(3593..>4143)
                     /locus_tag="VNQ33_08450"
     CDS             complement(3593..>4143)
                     /locus_tag="VNQ33_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98176.1"
                     /translation="ANLAFISVWIYSRTVGLPFGETPNAAESVGAIDTVCVLLQAGVI
                     LVALRLIIAPEKRSVGRLAPAMCAVAALGLATVVITSPDAANHSHGTTTAEPTGIDAL
                     RIKVDKERCDKDFNIPAYYEEAKYLGVDTRWGDNPPAVAGAPAATSADGHTHGAPAST
                     AASSTTTTEPDPYEGRGSPGLD"
BASE COUNT          555 a         1522 c         1459 g          607 t
ORIGIN      
        1 gagctggcgg gcgccggtga agagccgggt cctgcggctc atgtccatca ccaccccatc
       61 ggctcctgcg acggtgcgcc ggacctgccc gatcagcgcg gccaccacga cctcggtcgg
      121 atccaggggc cgcccgtcca cggtcccgca ccggtacccg atcaacccgc ccggcgtccc
      181 cggccgcggc gcccctcccc tgttggcatc ggcaccgacg ttcccgtccc cttccacacc
      241 tgcatcgacg ccggaatcgg cgtcggcggg tggaatcgga tcgacgccgg cgtgagggac
      301 aggttcggtg tcggcggctg gaaggcggcc ggcgggcgac ggcgatgcgc tgctgctcac
      361 cgcggggtcg aggtcgacga gggtggtggt gaggtcgtcc caccaggtgg cgaggcgggg
      421 atcggggccc gggtcggtgc cgaacagcat ggccaggcgg cgctcgaagg tgacgtcgtc
      481 caccaccagc tccgtgcacg tccgggcctc caccccgtac tcggcgaggt cgccgcgcag
      541 gaagcacgcc ttcatggcgt cgaagcggcg ctgcgcatcg gtgcgggcca gatcgtcctt
      601 ggtcgccgcg tcccccaagc ggcaacgggc atcggcccag tccgcctccc actcccgctt
      661 cacctgccgc tgccacacgt cttcgatctg ctgcatcgcc accccgcccc cgcctccgtc
      721 gaaccggccc gacccgtcga ggttccgcgg catccggaag tcccggttcg catcggcccg
      781 ctccgcccga tcccccgccc cgtcctcatc ggccaggcgc tcccagtcgg tgatcgcctc
      841 gtcgaagtct cggtacggca gcatcacggc cgccttcatc accgccgcgt cctccgccac
      901 catcgactcc cgcacccgct tgttggcgtg caccctcgcg atccgctccg cctgagccac
      961 gcccaaccgg cccgccgcgt gcgcagccga caccaccggg agatcccgca gcgccgacgc
     1021 cacctggctg cggcgcagcg cctccgcatt cgacaggttc ccgcagtggc gcaccatcac
     1081 cttcgccgac gcgtgcccat ccgccctgtg tgcaccagac cggtccactg ccgagagcac
     1141 ccccacctgc attgcatgga gccggcggcc gagcgcctcg gtctcccgga ttcccctggc
     1201 tgcatcatcg gcatctgccg gcacccaacc ctccagcgcc accgcgagct catcgagacc
     1261 cgcgctcacc cgctcgatcg cccccgtcat cacctggtcc tgcactgccg attccatgtg
     1321 cacatcatgc ggagggggtc tgacagtgag acagctcgaa acagcatgaa atcggacatt
     1381 tcaccgttgt ttttcatgga tcttgcccat tcctcaccgc ccggacgcag gctccacgcc
     1441 tggcatggga gcgggtcggg gcgggccact ggcatcgggt ggccgatccg tggtggaacg
     1501 ccacgttctc gacgttgcgt ggccgaaggc gggggtcgtg cccatccggt tgcggtccta
     1561 ccggtggcgg cccgtccaac cggacggcac ctcgaccgtg acggaccgag ccacggcccg
     1621 cgacgccatc gagaccccgc tccgcaccta ccttctccgc actccggagc cctgaccccc
     1681 atcgcccgcg acggggcgcc ggcgcggctg cacccgcccg ggccgtccga cgtcgtcggg
     1741 tcagtcggtg ccggtccgtt gggggatgcc cagggaccag aggaagccgg cgaggacatc
     1801 ggcatggcgg cggttcggga cgctgacctc caacgtggcg ccgtcggcca ggtccagcgt
     1861 cgcggggcgc tcgccgagca ccgagctgcc gccgatctcg taggccacca ccgcagcgag
     1921 cgggatgcga cgggtgcgca gcggcgcccg ccactccaac gatccgccct cgatccgcac
     1981 cgacaccgcg ctcctcagtc cgtaccgggt gacgatgagc gccaccaccg ccgtccagaa
     2041 cacggccccc ttcgggccgg cacccccgtc gccgtcgagg agcgatgcga tcgtcaccca
     2101 atcgatcaag agcgccaatg ccccgaccag caccaagccg agccagaacg cccagggcat
     2161 gccctcttgc cggtaggcat tcggcggcca cggaggaggc ggggccgagc gcaggcgcac
     2221 gcacatccac gtgcgccgtc cggcgagaaa ggtgcaagcc ccggtgagca gcgccaccaa
     2281 cggcgccacg atcagggcgc ggacgctggt cgcatcggtg tcctcgcacc acgcgggcga
     2341 cgcctcggtg cggccgagca gcgccgggca ctcggtcagc gtcacgttgc cgtggacccc
     2401 ctcgaccgtg taccagggcg taccctgcgg gagccgagcg gggtcgatgg tcgccgactg
     2461 cggttcgatc aggacgccga gcgccaccag cgcggcgatg gcgcacgtcg cacacgcgag
     2521 cagggtgacc acagcccaga gcccggtctg gatgaccgac ggccgccgca catccggacg
     2581 agcaggggcc tgctcgatca tcgccacggt cgtccatcgg ccagacccgg accgacctga
     2641 gcgaactcga ccggaacggt cagacgtcga ggcggggccg ggcgaatcgg gccgggcgcg
     2701 ctgcgatcgg cccgggcgca ggtcagtcgc cggtggtgtc gcctgtcgtc ttggcggcct
     2761 cgccggtggg ctcggcgcgc ttcttcgacg gcgggtcgtc catgccgtag cggtcgcgga
     2821 caccggagcc ggcgcagtgg ccgccgtcgg tgccggacga ggcgccgagc tggtcgtcga
     2881 ggagcgggct ggcggcgctg aacacgcccc acgggctctc gcagccctcc accacccagg
     2941 cgtggctcat ccaccccttg gagccgtcgt ctttggaacc gcctcgtgct tcgcagtcct
     3001 cgaccgtcgt ggtgctgtcg ccgatgatct ggccggtggt cgtgctcgag cagaggccga
     3061 tgtggcggtg ggcgtggtcg ttgtcgccgg tgaagccctg ggtgggcttg ttgtcgccgg
     3121 cgtgccactg gtagtagctc agccccacga cgtgggcgtc gaggccgttg ccgtcgtaga
     3181 ggatcatctc gggcttgtcg atctcgaacg tgtcgtcgat gaggctgccc ttgatccagt
     3241 gggcggcgat gccgggcacg tagccggtga cgagccggta gccggcgtcc ttggcgtctt
     3301 gcgccgtcgg gtacgtcatc gccgtgtcgc gggcgagctg cagctcggcg gcggtcttgt
     3361 cgcacagctt ctggtcggtc atggcgaccc agggctgggg gccgacgtga ccgccgtggc
     3421 cgctgccgtc gccagcggcg gaggtcgacg agtgctcgtg gctgagcgac cccgacccgc
     3481 ggagccacca gagccaggcg tcgtagtcct cgtcgctggc cttgctgagc gacgtgatga
     3541 ggacggctgc cgcgccctcg ctggtggccg cgaggccggt gtcggagacg agctagtcga
     3601 ggccgggcga gccccggccc tcgtaggggt cgggctcggt ggtggtggtg ctgctcgccg
     3661 ccgtcgaggc gggggcgccg tgcgtgtggc cgtcggccga ggtggcggcg ggagcgccgg
     3721 cgacggcggg cgggttgtcg ccccaccggg tgtcgacccc gaggtacttc gcctcctcgt
     3781 agtacgccgg gatgttgaag tccttgtcgc agcgctcctt gtccaccttg atccgcaggg
     3841 cgtcgatccc ggtgggctcg gcggtggtgg tgccgtggct gtggttggcg gcgtcgggcg
     3901 acgtgatgac gacggtggcc agcccgaggg cggcgaccgc gcacatcgcc ggggccagcc
     3961 ggccgaccga gcgcttctcg ggggcgatga tcaggcgcag ggccacgagg atgacgccgg
     4021 cttggaggag cacgcacacg gtgtcgatgg cgccgaccga ctcggcggcg ttcggcgtct
     4081 ccccgaacgg cagcccgacg gtgcggctgt agatccagac gctgatgaac gcgaggttgg
     4141 cca
//
LOCUS       DATMFS010000448         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_269295, whole genome shotgun sequence.
ACCESSION   DATMFS010000448 DATMFS010000000
VERSION     DATMFS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269295"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..932)
                     /locus_tag="VNQ33_08455"
     CDS             complement(264..932)
                     /locus_tag="VNQ33_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWJ98177.1"
                     /translation="MPASTMHRILVRHGINQLRWMDRPTGRVIRRIETSRCGELVHID
                     VKKLARMPDGGGHRKLGRTTETKRRSHVTRGGYTHIHTAIDAYSRLASRSSPAPRTPT
                     TAPRSSTGPSPGSTSTASPSSGSSPTTANGYRSIPWRERCIELGIKHSRTKPYHPATN
                     GRVERFNRTLADEWAYARLWKSEGSRALALDRFLHRYNHHRHHTAIGGPPASRVSNLA
                     GHNT"
     gene            975..1214
                     /locus_tag="VNQ33_08460"
     CDS             975..1214
                     /locus_tag="VNQ33_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWJ98178.1"
                     /translation="MAPVIALAVLVPVSLALAPHLRMVKLGDDLARNLDLEVELARAL
                     LLLVGVVLAAVATAVAGPIGFIALVAPRHGGRRER"
     gene            1214..1363
                     /locus_tag="VNQ33_08465"
     CDS             1214..1363
                     /locus_tag="VNQ33_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98179.1"
                     /translation="MTTDLVARVFHLRCEIIEDPTSGTPLILPISALERRAAPASASS
                     DRSSP"
     gene            1537..2535
                     /locus_tag="VNQ33_08470"
     CDS             1537..2535
                     /locus_tag="VNQ33_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98180.1"
                     /translation="MRALVTLAGAAVAVGSLAGCGGSSGSDSSSRPTSSIAAGGGASA
                     FVPFTVDHVYGRTEISERPERIVSLDPQWTDVLLAMGVTPVGYVTSSQVEGGGTFPWE
                     EDLLAGSEPISYTDGVPVEAVAALKPDLVLGTYVFTDQSDYDTLAGQFDVIAAPTDEQ
                     VQKWEDLTALTGKILGDPDRAEQVVDDVDAQVEQVAADLPGLKGKTIAFANYYPAGNQ
                     LVVLSDPDDGANVLFAQLGLELAPGIVALGDGANGRTELSLEQISALDGDILLALTNG
                     TDTADIVGWDRLPAVEAGTAQILDMSGAWALNTPSPLSIPWAIEEIRPTLEKAAGE"
     gene            2587..>3387
                     /locus_tag="VNQ33_08475"
     CDS             2587..>3387
                     /locus_tag="VNQ33_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIP domain-containing protein"
                     /protein_id="HWJ98181.1"
                     /translation="MARGFQGTMLRALGAAEHVLRVEGTADVVPGFRRVRFSSTDLFD
                     DFRPGATDYLRLWFPDPVDPGLEVQRGYTVVEPDIEAGTFVLDFVIHEPAGPAATWAQ
                     RCQPGDEIAATVYGSQAFAIGDPEPAGVLLVGDASSIPAINSILGVVPHHLPVEVVLE
                     WGHEHELAIPLAEHPKAVVQRVRRVPGATRVPEALSGRDWSDWKAWVGAERSTTKAVK
                     AALSDQLGFPKSEVKATAYWIHGRSFGGSRGAKAAAKAAAKEHEAVAGP"
BASE COUNT          461 a         1159 c         1239 g          528 t
ORIGIN      
        1 tcacgcaccg gtggactaaa ggtgacctcc ttctgtggcc gaacaggcga tatgcgcacc
       61 gcgtcctgcc gcttgaagga gcgagcacac ggtcgatgta tcgcgtcgtc ggaatctggg
      121 agtcttgacg ccgcaagggt cccgacgatc gcatgcgtca tcgcggatga gtgcacgggc
      181 cacgctcgct caggtcgaga gcgatgacag ggccgtccaa ttcgttcgcg ttcgcagggc
      241 aggcagcacc ccggcccgct cggctaggtg ttgtgtcctg ccaggttgct gacgcggctg
      301 gcgggtgggc cgccgatggc ggtgtggtgc cggtgatggt tgtagcggtg gaggaaccgg
      361 tcaagggcga gggcccggct gccttctgat ttccagagcc gggcgtaggc ccactcgtcg
      421 gcgagggtgc ggttgaaccg ctcgactcgg ccgttcgtgg ccgggtggta gggcttggtc
      481 cgtgagtgct tgatgccgag ctcgatgcag cgttcgcgcc atgggatcga gcggtagccg
      541 ttagccgttg tcggtgagga tccgctcgac ggtgatgccg tgctggtcga accaggcgac
      601 ggcccggtcg aggaacgcgg tgcagttgtc ggtgttctcg gtgccggcga actcctggag
      661 gcgagcctcg agtaggcgtc gatggcggtg tggatgtggg tgtagccgcc tcgcgtgaca
      721 tgcgagcggc gtttggtctc ggtggtcctg ccgagcttgc ggtggccgcc tccgtcgggc
      781 attcgggcca gcttcttcac gtcgatgtga accagttcgc cgcagcgact ggtctcgatc
      841 cggcggatca cccgcccggt cgggcggtcc atccagcgca gctggttgat gccgtggcgg
      901 accaggatcc ggtgcatcgt cgacgccggc acgtcgacga tccccgccag acgcgccggg
      961 ccgagcttgc ggtcgtggcc ccggtgatcg cgttggcggt cctcgtcccg gtgtcgctcg
     1021 cgctggcgcc gcacctccgg atggtcaagc tcggtgacga cctggcccgc aacctcgacc
     1081 tcgaggtcga gctcgcccgt gccctgctcc tgctcgtcgg cgtggtcctg gccgcggtgg
     1141 cgacggccgt cgccggaccg atcgggttca tcgccctcgt cgccccacgc cacgggggcc
     1201 gccgcgagcg gtgatgacga cggacctcgt ggcccgggtc ttccacctcc ggtgcgagat
     1261 catcgaggat cccaccagcg ggacgccgct gatcctgccc atcagcgcgc tcgaacggcg
     1321 cgcggcgccg gcatcggcga gcagtgatcg ctcctcgccg tgaccgcggg tcccgtcgtc
     1381 gccggaccgg gccatcggtc gcgacgcgcc gtgacccgcc gcgacggtcg cggcgtgctc
     1441 ggtgagggca tcggatgcaa gcttgttaag gtaccttgac attctatgtc agggagaggc
     1501 tcggtcatgc gggacgaaca ggccagaagg cgatcgatgc gtgcgctcgt cacgctcgca
     1561 ggagcggcgg tggccgtcgg ctccctggcc ggatgcgggg ggtcgagcgg ctcggacagc
     1621 tcgtcgaggc cgaccagctc gatcgccgcc ggtggaggcg cgtcggcgtt cgtcccgttc
     1681 accgtcgacc acgtgtacgg ccgcaccgag atctccgagc gcccggagcg gatcgtgagc
     1741 ctcgacccgc agtggaccga cgtcttgttg gccatgggcg tcacgccggt cggctacgtc
     1801 acgagctccc aggtcgaagg cggggggacg ttcccgtggg aggaggacct gctcgccggc
     1861 tccgagccga tctcctacac cgacggcgtg ccggtggagg ccgtggccgc gctcaagccc
     1921 gatctcgtgc tgggcaccta cgtgttcacc gaccagtccg actacgacac gcttgccggg
     1981 cagttcgacg tgatcgccgc gcccaccgac gagcaggtgc agaagtggga ggacctcacc
     2041 gcgctcaccg gcaagatcct cggcgatccc gaccgggccg agcaggtggt cgacgacgtc
     2101 gacgcgcagg tcgagcaggt cgcggccgac ctccccgggc tgaagggcaa gacgatcgcg
     2161 ttcgcgaact actacccggc cggcaaccag ctcgtcgtgc tctcggaccc cgacgacggc
     2221 gcgaacgtcc tgttcgccca gctgggcctg gagctggcgc ccggcatcgt cgccctcggc
     2281 gacggggcca acggccggac cgagctgagc ctcgagcaga tcagcgccct cgacggcgac
     2341 atcctcctcg cgctcaccaa cgggaccgac accgccgaca tcgtcggctg ggaccggctc
     2401 cccgccgtcg aagcgggcac cgcccagatc ctcgacatga gcggcgcctg ggcgctgaac
     2461 accccgtcgc cgctgtcgat cccgtgggcg atcgaggaga tccggcccac cctcgagaag
     2521 gccgcagggg agtagatcgg tgcgccgcct cggaaccgcc tgcgccggcc cagaccggag
     2581 ctgacggtgg ctcgcgggtt ccaggggacg atgctgcgcg cgctcggcgc cgccgagcac
     2641 gtgctgcggg tcgagggcac cgccgatgtg gtgccggggt tccggcgcgt gcgcttctcc
     2701 tcgaccgatc tgttcgacga cttccggccg ggcgccaccg actacctgcg cctgtggttc
     2761 ccggatccgg tcgaccccgg tctggaggtc cagcggggct acacggtggt ggagcccgac
     2821 atcgaggcgg gcacgttcgt gctcgacttc gtgatccacg agccagcggg cccggcggcc
     2881 acgtgggcgc agcggtgcca accgggtgac gagatcgccg ccacggtcta cggctcgcag
     2941 gcgttcgcca tcggcgatcc cgaaccggcc ggcgtgctgc tggtcggcga cgcgtcgtcg
     3001 atccccgcca tcaactcgat cctgggggtc gtgccccacc acctgccggt cgaggtggtc
     3061 ttggagtggg gccacgagca cgagctcgcc atccccctcg cggagcaccc gaaggcggtg
     3121 gtccagcgcg tgcgccgggt gccgggcgca acccgggtcc ccgaggccct gtcgggccgg
     3181 gactggtcgg actggaaggc gtgggtcggc gccgagcggt cgaccacgaa ggcggtcaag
     3241 gcggcgctgt cggaccagct cggcttcccg aagtcggagg tgaaggccac cgcctactgg
     3301 atccacggcc gctcgttcgg aggcagccgc ggcgccaagg ccgccgccaa ggccgccgcg
     3361 aaggagcacg aggccgtcgc cggtccc
//
LOCUS       DATMFS010000449         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_155186, whole genome shotgun sequence.
ACCESSION   DATMFS010000449 DATMFS010000000
VERSION     DATMFS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155186"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1499)
                     /locus_tag="VNQ33_08480"
     CDS             complement(9..1499)
                     /locus_tag="VNQ33_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HWJ98182.1"
                     /translation="MSWLVPIPIVLPLLGAALSILVGRSRPAQRVVGIGVLAVLVVVS
                     IVLLVQVDSHGTIVSDAGGWPGPLGITLVADRFAALLLLVAEVTLLVVLVYAIGEPGA
                     ERNHVGFQSAYLVLAAGVAASFLTGDLFNLFVSFEMMLTASYVLLTLGGRAEQVRSGM
                     TYIVISLLASTLFVMALALLYSATGTVNMADLALRMGELPPGVRTAFAVLLLVVFGIK
                     AAIFPLFFWLPDSYPTAPSPITAIFAGLLTKVGVYAIIRTQLLLFPPDSRPATLLLVL
                     AAATMVIGVLGAIAQDDIRRILSFTIISQIGYMVMGLAFFTVAGIAAVVFSIVHHIVV
                     KTSLFLVGGLVEHASGSNRLSRIGGMVRTAPLLAAMFLACALGLAGIPPFSGFISKLG
                     LIEAGIADRQYAVIAVALAVSLLTLFSMMRIWMGAFWSPREDDAPPAPEPTAARSPIL
                     MLVPTAVLVACSLGIAVAAGPIYSLSERTAHDLVDRDAYVEAVLGR"
     gene            complement(1496..1900)
                     /locus_tag="VNQ33_08485"
     CDS             complement(1496..1900)
                     /locus_tag="VNQ33_08485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HWJ98183.1"
                     /translation="MTVMLAATAAALFGIGTYLLLQRKLSRIIIGLGLLTHGANILLI
                     TAGRRGDPPLIGEGRVEGFADPMPQALALTAIVITFGVTSLLLALAYRSWLLTNDDEV
                     QDDLADRAVGDSDVVEEEVADQQQVIAREEAP"
     gene            complement(1897..>4225)
                     /gene="mbhE"
                     /locus_tag="VNQ33_08490"
     CDS             complement(1897..>4225)
                     /gene="mbhE"
                     /locus_tag="VNQ33_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HWJ98184.1"
                     /translation="IGNRHTEPRARAAALHALLVTGAGGLALLAGFVVLGREAGTFSL
                     SAILAAPPSGTAVTAAIALIIVGALTKSAQYPFHAWLPGAMAAPTPVSAYLHSATMVT
                     AGVFLVGRLAPSFADVVVWRPMILTAGCCTLVFGGLRALRQHDLKLLLAFGTVSQLGL
                     LMILFGVGTPASVTAGWELLVAHAAFKAALFMVVGIVDHQTGTRDLRKIPALGPSWNG
                     VRAVALLAVVSMAGVPLGAGFIAKELAYDALADGSFAGSSLVLAVAVAGSMLTVAYAA
                     RFYWGGFVHPNRRAADDTAAEPPAAPSWVMLAPPALLAVAGVVLGVAPWLLDHLATAS
                     LAGFRAGAKPVHLALWHGFNLPLALSALTLAGGALVFAADRWLRPWLALGSRVPSGGD
                     VYLAALRGLGRTSSRVTGVVQNGSLPVYAGVIILTAAALPAAALLSDWYWPGWPAFGA
                     GREAPIVGVLLIAATGAALARQRFSAALFLGVAGYAMAGLFIFSGAPDLALTQVTVET
                     LSTVVFVLVLRRLPERFERQSSPGRRAVRLAIAGAVALTVFGFSLITAGVRITPPVSE
                     QMVERALPDGHGHNVVNVILVDFRGFDTLGEITVLAVASLGAVALARVGRRAGSERGE
                     VQPAAIGPRRLPFVDVSVRIVFPAVVLMSLWLLFAGHNRPGGGFVGGLLAGSAITLRY
                     IAGGISEVRGRSRFRPWTVLGAGLLLAVGTAVAPLLAGGPVLDASLASFELPIIGAVK
                     VGSTLVFDAGVYLVVLGMVLMAFEAFGDPPAEAAS"
BASE COUNT          753 a         1453 c         1571 g          448 t
ORIGIN      
        1 cgtcagcctc accggcccag caccgcctcg acgtacgcat cgcgatccac caggtcgtgg
       61 gcggtgcgct cgctgagcga gtagatcgga ccagccgcca cggcgatccc caggctgcac
      121 gcgaccagca cggcggtcgg caccagcatc aggatcgggc tccgggcggc ggtgggctcg
      181 ggcgcaggtg gggcgtcgtc ctccctcggg ctccagaacg cgcccatcca gatccgcatc
      241 atggagaaca aggtgagcag gctgacggcg agggccaccg cgatgaccgc gtactggcgg
      301 tcggcgatgc cggcttcgat gagccccagc ttggagatga acccggagaa gggagggatg
      361 ccggccaggc cgagcgcgca ggcgaggaac atcgccgcca ggagcggtgc ggtgcggacc
      421 atgccgccga tgcggctgag ccggttggag ccgctggcgt gctcgaccag tccgccgacg
      481 aggaagagcg acgtcttgac cacgatgtgg tgcacgatcg agaacaccac ggcggcgatg
      541 ccggccacgg tgaagaacgc gagccccatc accatgtagc cgatctggct gatgatcgtg
      601 aacgacagga tccgccggat gtcgtcctgt gcgatcgcgc ccaggacccc gatgaccatc
      661 gtggccgccg cgagcacgag cagcagcgtg gcgggccggc tgtcgggcgg gaacaggagg
      721 agctgcgtgc ggatgatggc gtacacgccg accttggtga ggagcccggc gaagatggcg
      781 gtgatgggcg agggcgcggt ggggtagctg tcgggcagcc agaagaacag cgggaagatc
      841 gcggccttga tgccgaacac cacgagcagc aggacggcga aggcggtacg gacgcccggc
      901 ggcaactcgc ccatccgcag ggccaggtcc gccatgttca cggttcccgt cgccgagtag
      961 agcagggcga gcgccatcac gaagagcgtc gatgccaaga ggctgatcac gatgtaggtc
     1021 atgccggagc gcacctgctc ggcccgccct ccgagggtga gcagcacgta gctggccgtg
     1081 agcatcatct cgaacgacac gaagaggttg aagaggtcgc cggtgaggaa ggaggcggcg
     1141 acgccggccg ccagcacgag gtaggcggac tggaacccga cgtggttgcg ctccgctccc
     1201 ggctccccga tcgcgtagac gagcacgacg agcagcgtca cctcggcgac cagcaggagc
     1261 agggcggcga agcggtcggc gacgagcgtg atgccgagag ggccaggcca cccaccggca
     1321 tcggagacga tcgtgccgtg ggagtccacc tgcacgagca ggacgatcga gacgacgacg
     1381 agcaccgcca gcacgccgat gcccacgacg cgctgcgccg gccgcgaacg tccgaccagg
     1441 atcgacaggg cggcgccgag gagcggcagc acgatcggga tcgggacgag ccagctcacg
     1501 gggcttcctc ccgggcgatg acctgctgtt ggtccgcgac ctcttcctcc accacgtcgg
     1561 agtcgcccac ggcgcggtcc gcgaggtcgt cttggacctc gtcgtcgttc gtcagcagcc
     1621 agctccggta ggccagggcc aagaggagcg aggtgacgcc gaacgtgatc acgatggcgg
     1681 tcagcgccag cgcctggggc atcgggtcgg cgaacccttc cacccggccc tcgccgatca
     1741 gcggaggatc gccgcgccgg cccgccgtga tcaacaagat gttggcaccg tgcgtcagca
     1801 ggcccaggcc gatgatgatc cggctcaact tgcgctggag cagcaggtag gtcccgatgc
     1861 cgaacagcgc agcggcggtg gcagccagca tgacggtcat gacgccgcct ccgcaggagg
     1921 gtcgccgaag gcctcgaacg ccatgagcac catgccgagc accaccaggt agacgccggc
     1981 gtcgaagacc agcgtcgaac cgaccttcac ggcaccgatg atcgggagct cgaacgaggc
     2041 cagcgacgcg tcgagcaccg ggccaccggc cagcagcggg gcgaccgccg tcccgacggc
     2101 gaggagcagc ccggcgccga ggaccgtcca gggccggaac ctcgaccgcc cccggacctc
     2161 gctgatgccg ccggcgatgt agcggagcgt gatcgccgat ccggcgagca ggccgccgac
     2221 gaagccgccg ccgggccggt tgtggccggc gaacagcagc cacagcgaca tcaacacgac
     2281 cgcggggaac acgatccgca ccgacacgtc gacgaacggg agccgccgcg gcccgatcgc
     2341 cgccggctgg acctcgccgc gctcgctgcc agcccggcgg cccacccggg ccaaggcgac
     2401 cgcgccgagg gacgcgacgg cgaggacggt gatctccccg agcgtgtcga agccgcggaa
     2461 gtcgacgagg atcacgttga ccacgttgtg gccgtgaccg tcgggcaacg cccgttccac
     2521 catctgctcg gacacgggcg gggtgatccg gacgcccgcg gtgatcaggg agaagccgaa
     2581 caccgtgagg gcgacggcgc cggcgatcgc gaggcgcacg gcgcgccgcc ccggtgacga
     2641 ctgacgctcg aatcgctcgg ggagccgacg gagcaccagc acgaacacga cggtggagag
     2701 ggtctcgacc gtgacctggg tgagggcgag gtccggtgcc ccggagaaga tgaacaggcc
     2761 ggccatcgcg tagccggcga cgcccaggaa cagcgcggcg gagaagcgct gccgtgcgag
     2821 ggcggcaccc gtcgcggcga tgaggagcac gccgacgatc ggcgcctccc ggcccgcccc
     2881 gaacgccggc cagcccggcc agtaccagtc cgacaagagg gcggccgccg gcagggccgc
     2941 ggcggtgagg atgatgacgc ccgcgtacac cgggagcgac ccgttctgca ccaccccggt
     3001 cacgcgcgac gaggtgcggc cgaggccccg cagcgccgcc aggtagacgt cgccgccgct
     3061 cggcaccctg ctccccagcg ccagccacgg ccgcagccag cggtccgcgg cgaagacgag
     3121 cgcgccgccg gcgagcgtca gcgccgacag ggcgaggggc aggttgaagc cgtgccacag
     3181 tgcgaggtgc acgggcttcg cgccagcgcg gaagccggcg agcgacgccg tggcgaggtg
     3241 atcgaggagc cacggagcca cgccgagcac cacgccggcc acggccagca gcgcgggtgg
     3301 ggcgagcatc acccaagagg gcgccgccgg cggttctgca gccgtgtcgt cggcggcgcg
     3361 ccggttcggg tggacgaacc ctccccagta gaaccgggcg gcgtaggcga ccgtgagcat
     3421 cgacccggcg acggcgacgg ccagcacgag cgatgacccg gcgaacgatc cgtcggcgag
     3481 cgcgtcgtag gcgagctcct tcgcgatgaa cccggccccg agcggcacgc cggccatcga
     3541 caccaccgcg aggagcgcaa cggctcgcac gccgttccac gacggtccca gcgcggggat
     3601 cttgcgcagg tcacgggtcc cggtctggtg atcgacgatg ccgacgacca tgaacagggc
     3661 ggccttgaag gcggcgtggg ccacgaggag ctcccagccc gcggtcaccg acgccggtgt
     3721 gccgacaccg aacaggatca tcagcagccc gagctggctg accgttccga acgccaacag
     3781 cagcttgagg tcgtgctggc gcagggcgcg gaggcccccg aagaccagcg tgcagcagcc
     3841 ggcggtgagg atcatcggtc gccagacgac gacatcggcg aacgaggggg cgagccgtcc
     3901 caccaggaac acgccggcgg tgaccatcgt ggcggagtgc aggtaggcgc tcaccggagt
     3961 gggcgcggcc atggcgcccg gcagccacgc gtggaacggg tactgcgccg acttcgtgag
     4021 cgcgcccacg atgatcaggg cgatggcggc ggtgacggcg gtgcccgatg gcggtgcagc
     4081 caagatggcg ctcaggctga acgtgcccgc ttctcggccg agcacgacga agcccgccag
     4141 cagcgccagc ccgccggctc ccgtcaccag cagcgcgtgg agcgcggcgg cccgggctcg
     4201 tggctccgtg tgccggttcc cgatg
//
LOCUS       DATMFS010000450         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_110280, whole genome shotgun sequence.
ACCESSION   DATMFS010000450 DATMFS010000000
VERSION     DATMFS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110280"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /locus_tag="VNQ33_08495"
     CDS             complement(<1..1981)
                     /locus_tag="VNQ33_08495"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWJ98185.1"
                     /translation="MHLLAAEGGYQPFILGGSDKTLLILSLAIALLALGFSALMAKGV
                     IDSEDGTPEMQEIATAVQEGAMAYITRQFRTILMIVVPLCFVVFFTSAEILKDNNDTA
                     LSFTQSGLYRTLAFITGALFSGAIGFLGMWLATRGNIRTTAAATKSDYPGALRVAIRT
                     GGSVGLATAGLGLFGATTIILIFQNTSSAILVGFGFGGSLLALFLRVGGGIFTKAADV
                     GADLVGKVEAGIPEDDPRNPATIADNVGDNVGDCAGMASDLFESFGVTLVASIILGVS
                     AFRAINVSQADAAKGLVFPVAVMAFGLVASLIGIYLVKARPGESDALQAINRGIGVAQ
                     VLAIVGAAALAFGYVGNDPITEPGKQLAVSNPGARMFGAVLVGVVLGFAASKITAYFT
                     STTTKPVQDIAKATRTGPATTVLEGMSLGLESAVWALIAVAVAIGGAVALGGGSFKFS
                     VYLVALAGIGMLSTTGIIVAEDTFGPVADNAAGIAEMSGNFEGEPEKIMVALDAVGNT
                     TKAVTKGFAIGSAVIAAVALFASYGETIIEQLGGLWGRGASPGGESFVINVADPKVFI
                     GLLVGGSIAFIFSSLAIRAVSRTAGVVVQEVRRQFADGKIMSGEKKPDYGPAIDICTA
                     ASLRELATPAIIAVLAPVIIGFGLGPISLGAFLASV"
     gene            complement(2268..>2517)
                     /locus_tag="VNQ33_08500"
     CDS             complement(2268..>2517)
                     /locus_tag="VNQ33_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98186.1"
                     /translation="DPSAGGVTTPFVRLPGLVSGACESTGGFHYLAATVHPDPGPRAD
                     DIVGDLTPQWGLHLVDVNLVMGNLVSDVKAQAKTYAGN"
BASE COUNT          413 a          791 c          963 g          350 t
ORIGIN      
        1 tgaccgacgc caagaaggcg ccgagcgaga tcgggccgag gccgaagccg atgatcaccg
       61 gggcgagcac ggcgatgatg gccggcgtgg ccagctcgcg gagcgaggcg gcggtgcaga
      121 tgtcgatggc ggggccgtag tcgggcttct tctcgccgct catgatcttg ccgtcggcga
      181 actggcggcg gacctcctgg accaccacgc cggccgtgcg ggacacggcg cggatggcga
      241 gcgaggagaa gatgaaggcg atcgagccgc cgacgaggag gccgatgaag accttcgggt
      301 cggccacgtt gatgacgaac gactcgccac cgggcgaggc gccccggccc cagaggccgc
      361 cgagctgctc gatgatcgtc tcgccgtagg aggcgaacag ggccacggcc gcgatgaccg
      421 cggagccgat ggcgaagccc ttggtgacgg ccttggtggt gttgcccacc gcgtcgagcg
      481 ccaccatgat cttctcgggc tcgccctcga agttgccgga catctcggcg atgcccgcgg
      541 cgttgtccgc caccgggccg aaggtgtctt cggccacgat gatgccggtg gtggacagca
      601 tgccgatgcc ggcgagggcc accaggtaga cggagaactt gaacgagccg ccgccgaggg
      661 ccacggcgcc gccgatggcg acggccacgg cgatgagggc ccacacggcc gactcgaggc
      721 cgagggacat gccctcgagg accgtggtag ccgggccggt gcgggtcgcc ttggcgatgt
      781 cttggaccgg cttggtggtg gtggaggtga agtaggcggt gatcttcgag gcagcgaagc
      841 cgaggaccac accgacgagc acggcaccga acatgcgggc gccggggttg ctcaccgcca
      901 gctgcttccc gggctccgtg atgggatcgt tgccgacgta gccgaacgcc agggcagccg
      961 caccgacgat ggcgaggacc tgggcgacgc cgatgccgcg gttgatggcc tgcagcgcat
     1021 cggactcgcc cggccgggcc ttgaccaggt agatgccgat cagcgaggcg accaggccga
     1081 acgccatgac ggcgacgggg aacaccaggc ccttggccgc atcggcctgg ctcacgttga
     1141 tcgcccggaa ggcgctgacg ccgaggatga tcgacgccac gagggtcacg ccgaaggact
     1201 cgaagagatc cgaggccatg ccggcgcagt cgccgacgtt gtcgcccacg ttgtcggcga
     1261 tggtggccgg gttgcggggg tcgtcttcgg ggatgccggc ttcgaccttg ccgacgaggt
     1321 cggcgccgac gtcggcggcc ttggtgaaga tgccgccgcc gacgcgcagg aacagggcca
     1381 gcagcgaccc gccgaagccg aagccgacga ggatcgccga gctggtgttc tggaagatca
     1441 ggatgatcgt ggtagcgccg aacagaccca ggccggcggt ggccaggccg accgagccgc
     1501 cggtgcggat ggcgaccctg agagctcccg ggtagtcgct cttggtggcg gcggcggtgg
     1561 tgcggatgtt gccgcgggta gcgagccaca tgccgaggaa gccgatggcc ccggagaaca
     1621 gcgcaccggt gatgaacgcc agcgtccggt agaggccgga ctgggtgaag ctcagggcgg
     1681 tgtcgttgtt gtccttgagg atctccgccg aggtgaagaa caccacgaag cacagcggga
     1741 ccacgatcat gaggatcgtg cggaactggc gcgtgatgta cgccatcgcc ccctcttgga
     1801 ccgcggtggc gatctcctgc atctccggcg tgccgtcctc ggagtcgatg acgcccttcg
     1861 ccatgagggc ggagaagccg agggccagca gggcgatggc cagggacagg atgaggaggg
     1921 tcttgtcgga ccctcccagg atgaagggct gatagccgcc ttcggctgcg agcaggtgca
     1981 ccgtggatcc ttcgtcgggt tcggcgttgg tacaggagcg tctgccaggg ccgcgtcgca
     2041 ccacccgaac gggtggcgcg gggcgcgagc gagtccccgc agaacggctc ttcgctacta
     2101 cgcccgacgg gcgtgacgca agatgacaca agggttcgcg gggcgtgaga cctcctgtca
     2161 ggcccgcgtc agaccgagtt caggcatcgg gcgcgggcgg cggtgcgccc ggtcgagatc
     2221 ggcgcgcttc gttcggatcc ggtcgctgct gccggacgag ccggagctca gttgccggcg
     2281 taggtcttgg cctgggcctt cacgtcggag acgaggttgc ccatcaccag gttcacatcg
     2341 acgaggtgga gcccccactg cggcgtcagg tcgccgacga tgtcatcggc ccgcggcccg
     2401 ggatcggggt ggaccgtggc cgccaggtag tggaagccgc cggtcgactc gcacgcgccg
     2461 ctgaccagcc ccggcagccg gacgaagggg gtggtgacac cgcccgcgga cgggtcg
//
LOCUS       DATMFS010000451         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_318703, whole genome shotgun sequence.
ACCESSION   DATMFS010000451 DATMFS010000000
VERSION     DATMFS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318703"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VNQ33_08505"
     CDS             <1..536
                     /locus_tag="VNQ33_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98187.1"
                     /translation="LSEVIGRKIAGVAADSLVLYNQPAGTDWLPTSNIELEAEGRFGS
                     SITRSCPTFDDPPNGDNPESPENLEWARERNRCEAPWVLRLLDPAQGQSVHLYAVAGG
                     LALGIAGSAIVSVVALLIAGVWGRRPNLLPSTPDTRSERSRQRSQQSTNPLLTGAVMV
                     LGVLLARRRRGQQHPSD"
     gene            complement(1668..2063)
                     /locus_tag="VNQ33_08510"
     CDS             complement(1668..2063)
                     /locus_tag="VNQ33_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98188.1"
                     /translation="MSEDVELVVYGSELGRAVWRGGQHEVVRVVPTGTPIADATEGVD
                     AALCILDIDVANDLLAAGIPTVYVDSLPFPWRPADGVPLHATKYLAQLTPDLPATCWD
                     VLSEIECLEWIEGIVPADGTWSPTSDRIV"
     gene            complement(2257..2329)
                     /locus_tag="VNQ33_08515"
     tRNA            complement(2257..2329)
                     /locus_tag="VNQ33_08515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2294..2296),aa:Ala,seq:tgc)
     gene            2566..3498
                     /locus_tag="VNQ33_08520"
     CDS             2566..3498
                     /locus_tag="VNQ33_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98189.1"
                     /translation="MGIPRETGDGQPVDDAGDGIVIDITDAAMARRAAAEEEALLSVA
                     ADGELILDLTDDALAAAEKAEAAKAAREAAARVVSGRPNDLLEDHRLVTITGGDEWMN
                     AEAFVYERYVSLGYADHSSRKRVEELARWHDHSRFHAVVDDQGAFVGTVRTIFGPYQE
                     LPVGQFERTNFDDPNPLCELSSLVVRQDFGGSGVLDHLFRAGWADALRLGARGIVALI
                     DTWIFDVLCDLYCMPFSQLGEPHFHMGGDVVPVVMSTSQSAMSQIAHHNPDFYRWNME
                     ALTPDEIRRFGLNNLSGIDVLAERADAERADAER"
     gene            complement(3578..4057)
                     /locus_tag="VNQ33_08525"
     CDS             complement(3578..4057)
                     /locus_tag="VNQ33_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98190.1"
                     /translation="MEQMTGTSSAGSDARFPDKPRTKLRKRLELSWVISIVAFTLARF
                     FVARETLEQYGLNIWVFGFIDLITAIPYAVGTARVVGALVDRDLTGASKWAGIAAVSF
                     FAPYAYILWAGSEDGATFPTSVYVVLGVLIAIFGANAVWGVVRKVRTARAAAHAAAV"
     gene            4223..>4859
                     /locus_tag="VNQ33_08530"
     CDS             4223..>4859
                     /locus_tag="VNQ33_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98191.1"
                     /translation="MRARIWIVFLGVGAALGCAAALLGQDAQSIVALPLGIAASAIGL
                     TTSRRAVNEEARTTWTYFGLAGCGFLLAGITRASLGAVAPELKAYPSPADIWIALAYV
                     CLILGTYQMGTIRSFGKNMAAHLDGLIVAIGSATAVWALVLWPYMVNDSQPLTDRLVN
                     AGFSILTTILLAVTVRLAVGPGVRSFPYYLLACAVGLLFICDIAATLASADG"
BASE COUNT          815 a         1618 c         1651 g          775 t
ORIGIN      
        1 acctctccga ggtcatcggg aggaagattg cgggcgtggc ggccgattca ctcgttctct
       61 acaaccagcc agcaggcacc gactggttgc cgaccagcaa tattgaactc gaagcggagg
      121 gtcgctttgg ttcaagtatc actcgctcct gccccacctt cgacgatcca ccaaacggcg
      181 ataatccaga gtccccggag aacctcgagt gggcccgtga acgcaaccgc tgcgaggcgc
      241 cgtgggtgct ccggctgctg gatccagcac agggccaatc ggtccacctc tacgcagtag
      301 caggcggtct cgctcttgga atcgcaggat ctgcgatcgt gtctgtcgta gcgctcctca
      361 ttgccggcgt gtggggaagg cgaccgaatc tcctaccgtc gacaccagac acccgaagcg
      421 aacgctctcg ccaacgaagc caacaatcca ccaatccgtt gctgaccggg gcggtgatgg
      481 tcctgggggt actcctggcc aggcgtcgca gagggcagca gcacccctca gactgacgtc
      541 ccgtgcacgt gtggttgtca gcatcaccag gagcgctggc aatcgccagc ggcgatctcc
      601 gtctcctgac caacgaggcc gtcagccatt ctccggcgat gcatcgccgg ggttccttgc
      661 cccagaatcg gccgagaact cagcagccca tccccgagcg aggccgtgtt gtgctggctc
      721 gccctcacgg catgggcgta gaccgccagg gtggtggtcg agaccgagtg accgaaccgc
      781 tcggagacgt tggtgatcgt ctcgccggca aggagcagga gagaggcatg ggtgtggcgg
      841 aggaggtgcg gctgttggct tccggtcgag tccgatctcg gtctcccagg ggaggtgtcg
      901 agcgacgaag tcgttgagct tgtcggggtc ctcggtcggc cgtcctcgtg gcagaggacg
      961 aggtgatcgc cgacccccgc gatggagtcg gagttgatcg agcaacaagg gcgacacgga
     1021 gatcgtccgt tcagactcaa gcgtcttgag ctgagccagc ttcaccggtc cggttttggg
     1081 tcgaactgcc tgttcgacaa acgagaccgt tccgcgctcg aagtcgatgc cggcccaccg
     1141 gatgccgaag atctcctcga cgtgcccggg tctcgagact gcacaacaga gcgagtgcgc
     1201 tctgggacgc agcggccagt tctcgacgag ccatgctcgg agccgggctg cttcttccgg
     1261 ttgaacggca cgacggtggt cttgtcgcac cgaccggctc gacccggcac cggagcgagc
     1321 gtgaccacgt ttcggtcgag aacctcccag cggagcgctg cctgggttgc cagtgacaac
     1381 gtcccgcgcc ggcttcgagc aacgtcgtga gacctggaga ggtgagcagg agcgaggcag
     1441 atcgggccaa gtcgagaaac gattcgtgcg gcagcgattc gacggtgacc ccgtggaagc
     1501 ccagggggat ggcatcagcg ataacgccta gagcgtcgat gctcgtggtc acagtgaccg
     1561 gtacgtctcc acaggtagac gcgatagctg caagcgcggg cggtacgact acgtacggat
     1621 aggcgatctg atcggcgggc ggacccagga ggtcaaacca cccaggttca gacaatccga
     1681 tctgacgtcg gcgaccacgt cccgtcggcg ggcacgatcc cctcgatcca ctccaggcac
     1741 tcgatttcgc tcagcacatc ccaacaggtt gccggcagat ccggagttag ctgggcgagg
     1801 tacttggtcg cgtgcagcgg aacgccgtcg gccggacgcc aggggaacgg cagactgtcg
     1861 acgtacaccg tcgggattcc ggcggccaat aggtcgttgg ccacatcgat atcgaggatg
     1921 cacagcgcag catctacgcc ctcggtcgca tcggcgatgg gagttcccgt cgggaccacg
     1981 cgcacgacct cgtgctggcc cccacgccat acggcccggc cgagctcgct gccgtacacc
     2041 accaactcaa catcttcgga cacctcggcg aggacggagg cgagcttgcc ggcgctcccg
     2101 aggccgaaga tgtggcgggg cagcgcaggg caccgatcac ttccgggcga tttcgcccga
     2161 gatttggggc cggattggac gccgattgga caaacgggct tggtggccgg tcggtctcgc
     2221 cgacgtgacg ttgtgcttga cctgcggatc ttcgagtgga gctgatcgga atcgaaccga
     2281 cgacatctgc cttgcaaagg cagcgctctg ccaactgagc tacagccccg ggcgggaccc
     2341 atcaggggcg acccggccga gcgaggttag ccagtggcgg tgccgggtcg gtagcccgat
     2401 cggtcgacgc acacggtggg cgggccgtga cgctgcgcct tgcctggcca cggtccaacg
     2461 cggctcggcg gtcgagccgc tacggagcga gacgggtgat gcgacattcc caccgtgggt
     2521 gacctacgct tcgccgtagg tacacgcatg tctggagggg cagctgtggg gattccgcgg
     2581 gaaacgggcg atggtcagcc ggtcgacgac gccggtgacg gcatcgtcat cgacatcacg
     2641 gatgccgcca tggcgcgccg tgccgccgcc gaggaggagg cgctcctgtc ggtcgcggcc
     2701 gacggcgagc tgatcctcga cctcaccgac gacgccctcg ccgcggccga gaaggccgag
     2761 gcggcgaagg cggccaggga ggccgcggcc cgcgtcgtca gcggccgccc gaacgacctg
     2821 ctcgaggacc accggctggt caccatcacc ggcggcgacg agtggatgaa cgccgaggcg
     2881 ttcgtctacg agcgctacgt gagcctcggc tacgccgacc actcgagccg caagcgcgtg
     2941 gaggagctcg cccgctggca cgaccactct cgcttccacg ccgtcgtcga cgaccagggc
     3001 gcgttcgtcg gcaccgtccg cacgatcttc gggccctacc aggagctccc ggtgggccag
     3061 ttcgagcgca cgaacttcga cgatcccaac ccgctgtgcg agctgtcgtc gctcgtcgtg
     3121 cgccaggact tcggcggcag cggtgtgctc gaccacctgt tccgggccgg ttgggccgac
     3181 gccctgcgcc tcggcgcccg cggcatcgtc gccctcatcg acacgtggat cttcgacgtg
     3241 ctgtgcgacc tctactgcat gcccttctca cagctcggcg agccgcactt ccacatgggc
     3301 ggcgacgtcg taccggtggt gatgtcgacg agccagtcgg cgatgtcgca gatcgcgcac
     3361 cacaacccgg acttctaccg gtggaacatg gaggcgctga cgcccgacga gatccgccgc
     3421 ttcggcctca acaacctcag cggcatcgac gtgctggccg agcgcgccga cgccgagcgg
     3481 gccgacgcgg agcgctgaac gctgctcgag ggctgacgcc gaccagccgg cggcccggcc
     3541 tcgacccggg cgttcgagcc cggtccggcg gcaccgatca gacggcggcg gcgtgggccg
     3601 cggcgcgggc ggtgcggacc ttgcggacga cgccccacac ggcgttcgcc ccgaagatgg
     3661 cgatgaggac gccgagcacg acgtagacgc tcgtggggaa cgtggcgcca tcttcggaac
     3721 cggcccagag gatgtaggcg tagggggcga agaagctcac ggcggcgatg ccggcccact
     3781 tgctggcacc cgtcaggtcc cggtcgacga gcgcaccgac cacgcgcgcg gtgccgaccg
     3841 cgtacgggat ggcggtgatc aggtcgatga agccgaagac ccagatgttg aggccgtact
     3901 gctcgagggt ttcacgcgcc acgaagaagc gcgccaaggt gaacgccacg atgctgatga
     3961 cccaggagag ctcgaggcgc ttgcgcagct tggtgcgcgg cttgtcaggg aagcgcgcgt
     4021 cggatcctgc ggatgatgtg cccgtcatct gctccatcag gacgtcccct cagtcaccct
     4081 gcgtggcgcc atggtagctg ctcgtgagcg gaacggacat atctgggtcg aatggcctgt
     4141 tcaggccccc aggaggcacg acaggcacgt tcccatgagt caggctgtag gacggctgac
     4201 tgcggccgac gagtagaggt acatgcgcgc gagaatctgg atcgtcttcc tcggtgtggg
     4261 cgccgcgctg ggctgtgctg cggcactgct cggccaggac gcacagagca tcgtcgcgct
     4321 cccgctcggc atcgccgcca gcgcgatcgg gctcacgacg tcgcgccggg ccgtcaacga
     4381 ggaggcccgc accacgtgga cctacttcgg gctggccggg tgcggcttct tgctcgcggg
     4441 catcacgcgc gcctcgctcg gtgccgtcgc acccgagctg aaggcctacc cgtcgccggc
     4501 cgacatctgg atcgccctcg cctacgtgtg cctgatcctg ggcacgtacc agatgggcac
     4561 catccggtcc ttcggcaaga acatggcggc gcacctcgac gggctcatcg tcgccatcgg
     4621 ctcggcgacg gccgtctggg ccctggtgct gtggccctac atggtcaacg acagccagcc
     4681 gctcactgac cgcctggtca acgccggctt ctcgatcctg accacgatcc tcctggccgt
     4741 caccgtgcgg ctggcggtgg gacccggtgt ccggtcgttc ccctactacc tgctggcctg
     4801 cgcggtcggg ctgctcttca tctgcgacat cgccgccacg ctcgcctcag ccgacggcc
//
LOCUS       DATMFS010000452         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_15575, whole genome shotgun sequence.
ACCESSION   DATMFS010000452 DATMFS010000000
VERSION     DATMFS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15575"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VNQ33_08535"
     CDS             <1..639
                     /locus_tag="VNQ33_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ98192.1"
                     /translation="EADQHGVFPLDDRTVELFGARFRDHSPHASNRRYTYYPPMSPMP
                     GQVGPPLAGLGWDLMATIGDRAEGSGGVIYAVGTENSGISLFVQDDRLTFDYNAFGDH
                     TIVTSEVPVPVGASVIGLEFRRTATGGRAELVVDGERVGAADIPFVMFMMSSIGPSVG
                     YDHGSAVSAAYEAPFRFEGDLQRVDIQLYRSFDELTAKAEAEARARAELSRQ"
     gene            complement(654..>2183)
                     /locus_tag="VNQ33_08540"
     CDS             complement(654..>2183)
                     /locus_tag="VNQ33_08540"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ98193.1"
                     /translation="ARYDPAMTDVADAPLPSADEAEHLDVLVIGAGLSGIGAAYRLQT
                     MCPATTYAILEGRSALGGTWDLFRYPGIRSDSDMFTLGYPFRPWTGAKSIADGADIWA
                     YLDDTAKEYGIDRHIRYEHRAVSASWSTEDARWTVEATVGPEATPARVTCRFLYLCSG
                     YYSYERGYQPDFPGREEFTGEVVHPQAWPEDLDDAGKRVVIIGSGATAVTLVPAMAET
                     AGHVTMLQRSPTYIATLPGRDPFADLVRKLLPAKAAHSIVRWKNVLFTSAFYQFCRRR
                     PKAARRVMRRMVAAQLPKGYDTDPDFNPRYDPWDQRLCLVPDGDLFRCIKRGTADIVT
                     DTIERFTPTGIQLRSGRHLDADVIVSATGLNLQAGGGMAVEVDGQAVDLGDTFVYKGF
                     MLSDVPNLAMCVGYTNASWTLRADISSQEVCKVINHLDRHGYRSATPTVREHIDKVPI
                     LDLSSGYVQRGKDVMPQQGTKAPWVLRQNFVLDLLSSKLGDVTEGLVFDRPADREPAR
                     R"
BASE COUNT          319 a          737 c          790 g          337 t
ORIGIN      
        1 gaggccgacc agcacggggt gttcccgctc gacgaccgca ccgtcgagct gttcggcgcc
       61 cgcttccggg accactcgcc ccacgcgtcg aaccgccgct acacgtacta cccgccgatg
      121 tcgccgatgc cgggccaggt cggtccgccg ctcgctggtc tgggctggga cctgatggcc
      181 accatcggtg accgggccga ggggagcggt ggcgtgatct acgcggtcgg caccgaaaac
      241 agcggcatct cgctcttcgt ccaagacgac cggctgacgt tcgactacaa cgcgttcggc
      301 gaccacacca tcgtgacctc cgaggtcccg gtgcccgtcg gtgcgtcggt gatcgggctg
      361 gagttccgtc ggaccgccac cggcggacgg gccgagctgg tggtcgacgg cgagcgggtc
      421 ggcgccgccg acatcccgtt cgtgatgttc atgatgtcga gcatcggacc gagcgtcggg
      481 tacgaccacg gctccgcggt gagcgcggcg tacgaggccc cgttccgctt cgagggcgac
      541 ctccagcggg tcgacatcca gctctaccgc agcttcgacg agctcacggc gaaggccgaa
      601 gccgaagccc gagcacgagc cgagctctcc cggcagtgat caccgcccgt tgatcaacgc
      661 cgggccggct cccggtcggc cggccggtcg aacacgaggc cctcggtcac gtcgccgagc
      721 ttcgaggaca gcaggtcgag cacgaagttc tggcgcagca cccacggcgc cttggtgccc
      781 tgctggggca tgacgtcctt gccgcgctgc acgtagcccg acgacaggtc gaggatcggg
      841 accttgtcga tgtgctcgcg gaccgtgggc gtggcggacc ggtagccgtg gcggtcgagg
      901 tggttgatca ccttgcacac ctcctgcgag ctgatgtcgg ctcggagggt ccacgaggcg
      961 ttcgtgtacc cgacgcacat cgcgaggttg ggcacgtcgc tcagcatgaa ccccttgtac
     1021 acgaaggtgt cgccgaggtc gacggcctgg ccgtcgacct cgaccgccat gccgccgccg
     1081 gcctgcaggt tgaggcccgt ggccgacacg atcacgtcgg catcgaggtg ccggcctgac
     1141 cgcagctgga tgccggtcgg tgtgaaccgc tcgatcgtgt cggtgacgat gtcggccgtg
     1201 ccgcgcttga tgcagcggaa caggtcgccg tcgggcacca ggcacaggcg ctggtcccac
     1261 gggtcgtacc tcgggttgaa gtccgggtcg gtgtcgtagc ccttggggag ctgggcggcg
     1321 accatccgcc tcatcacgcg ccgggcagcc ttcggacgtc gtcggcagaa ctggtagaag
     1381 gccgacgtga agagcacgtt cttccagcgc acgatcgagt gggccgcctt cgcagggagg
     1441 agcttgcgca ccaggtcggc gaacgggtcc cgtccgggca gggtggcgat gtaggtcggc
     1501 gaccgctgga gcatcgtgac gtgccccgcc gtctcggcca tcgctggcac gagcgtgacc
     1561 gccgtggcgc cgctgccgat gatgacgacg cgcttgccgg cgtcgtcgag gtcctcgggc
     1621 caggcctggg ggtggaccac ctcgccggtg aactcctcgc gcccagggaa gtccggctgg
     1681 tagccgcgct cgtagctgta gtacccgctg cagaggtaga ggaagcggca ggtcacccgg
     1741 gccggggtgg cctccggacc cacggtggcc tcgacggtcc accgggcgtc ctcggtgctc
     1801 cacgacgccg acaccgcccg gtgctcgtag cggatgtgcc ggtcgatgcc gtactccttc
     1861 gccgtgtcgt ccaggtacgc ccagatgtcg gcgccgtcgg cgatggactt cgccccggtc
     1921 caggggcgga acgggtagcc gagcgtgaac atgtcggagt cggagcggat gcccgggtac
     1981 cggaacaggt cccaggtgcc gccgagggcg gatcggccct cgaggatcgc gtacgtcgtc
     2041 gccggacaca tcgtctggag ccggtacgcc gcgccgatgc ccgagagccc agccccgatg
     2101 accaagacgt cgaggtgctc ggcctcgtcg gcggaaggga ggggagcgtc ggccacgtcg
     2161 gtcatggctg gatcgtaccg agc
//
LOCUS       DATMFS010000453         4440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_269405, whole genome shotgun sequence.
ACCESSION   DATMFS010000453 DATMFS010000000
VERSION     DATMFS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269405"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VNQ33_08545"
     CDS             complement(<1..482)
                     /locus_tag="VNQ33_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ98194.1"
                     /translation="MDEGHPAAADRAPSWWPWACGAVAVVAAFNACWALGTADWKTDE
                     AWDGRLAWLAIDRGDWTQDNGHPIFVRWFLGLGQLLLGQTRTGIRLLPALASLAAVGL
                     LYLVGARLHSRWAGLVAAALWAVLPRALVVGSTVVAPLRGDRFGYLEPFMAVALLGAT
                     "
     gene            complement(602..1135)
                     /gene="ilvN"
                     /locus_tag="VNQ33_08550"
     CDS             complement(602..1135)
                     /gene="ilvN"
                     /locus_tag="VNQ33_08550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWJ98195.1"
                     /translation="MATDIEHHTLSVLVENKAGVLARIVNLFSRRAYNIYSLAVAPTE
                     DERFSRLTVVVDVDSAPLEQIVKQLHKLINVVKIVELDPSHSVERELMIATVRCPGDQ
                     RHQVTELVRIFEGKILAVNQQALTVSLEGHPSKVDDFEEMLRTFGISDIQRTGRIALP
                     KLDRDAGKLRAVKNKSA"
     gene            complement(1180..2931)
                     /locus_tag="VNQ33_08555"
                     /pseudo
     CDS             complement(1180..2931)
                     /locus_tag="VNQ33_08555"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            complement(3156..3569)
                     /locus_tag="VNQ33_08560"
     CDS             complement(3156..3569)
                     /locus_tag="VNQ33_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ98196.1"
                     /translation="MTRVLVDTSVVNRFASDLVAIEIEDAVEAGDVAVCAPVIFEVCF
                     SAQNGAAYERLRRQFDALPQVRTHQAVFDRAIEVQGALCAQGRHRGVSMADLLIAAAA
                     EAADLPVVHYDADFDLIAEVTGQPSRWVVPRGLID"
     gene            complement(3566..3769)
                     /locus_tag="VNQ33_08565"
     CDS             complement(3566..3769)
                     /locus_tag="VNQ33_08565"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWJ98197.1"
                     /translation="MAKTAINIDRDLADRAGELLGTETLTATVEEALREVVARAERKA
                     AFQWFRDHPFTPEERELMNRASR"
     gene            complement(4031..>4440)
                     /locus_tag="VNQ33_08570"
     CDS             complement(4031..>4440)
                     /locus_tag="VNQ33_08570"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWJ98198.1"
                     /translation="DIAPGTVIVIRYEGPKGGPGMREMLAITGALKGAGRGSDCALIT
                     DGRFSGGTWGFCIGHVAPEAVDGGPIAFVRDGDRIVIDVHQFRLDLMVDEVELEARKV
                     GWTPNPPRYTQGVLGKYARLVQGAETGAITNTK"
BASE COUNT          664 a         1456 c         1625 g          695 t
ORIGIN      
        1 cacgtcgccc cgaggagggc caccgccatg aacggctcga ggtacccgaa ccggtcgcct
       61 cggagcggcg cgacgacggt cgagcccacc accagcgccc gaggcagcac agcccacagc
      121 gcggcggcca cgagccccgc ccaccgggag tgcagccggg cgccgaccag gtagagcagc
      181 ccgactgcgg cgagcgaggc gagcgccgga aggaggcgga tgcccgtccg ggtctggccg
      241 agcagcagct gccccaggcc gaggaaccag cgcacgaaga tcggatgccc gttgtcctgg
      301 gtccagtcgc cccggtcgat ggcgagccaa gcgaggcggc cgtcccaggc ctcgtcggtc
      361 ttccagtcgg ccgtgccgag cgcccagcac gcgttgaacg cggcgacgac cgccaccgct
      421 ccgcacgccc acggccacca cgacggcgcc cgatccgcgg cagcggggtg cccctcgtcc
      481 atcacgccgc gaacctaggg cagcccggct gcgcccgagc gccccgaccc caccgaactt
      541 cgtaggtgag gacgcccatt cgggggcgct cgcctacgaa gttcgttcgg cgtggggggg
      601 gtcaggcgga cttgttcttg accgcccgga gcttgccggc gtcgcggtcg agcttcggca
      661 gggcgatgcg accggtgcgc tggatgtcgc tgatgccgaa cgtgcgcagc atctcctcga
      721 agtcgtccac cttcgacggg tggccttcga gcgagacggt gagcgcctgc tggttcacgg
      781 cgaggatctt gccctcgaag atgcggacca gctcggtcac ctggtggcgc tggtcgcccg
      841 ggcaccggac ggtggcgatc atgagctcgc gctcgacgga gtgcgagggg tcgagctcca
      901 cgatcttcac gacgttgatg agcttgtgga gctgcttgac gatctgctcg agcggcgccg
      961 agtccacgtc gaccacgacg gtcaggcggc tgaagcgctc gtcctcggtg ggcgcgacgg
     1021 ccagcgagta gatgttgtac gcccggcggg agaacaggtt gacgatgcgg gcgagcacgc
     1081 ccgccttgtt ctcgaccagg acggagaggg tgtggtgctc gatgtcggtc gccatcagtc
     1141 cgcggcctcc tcggcgcccc gggcccgctg gaacggcggg acctggacat cggagttgga
     1201 gtggccggcg gccaccatcg ggaagacctt ctccgacgca tcggtgcgga actcgatgac
     1261 cacggggcgg tcgtcgatgg cgttggcctt gtcgatggtg gggccgatgt cgtcggggtg
     1321 ctcgacccgg aaggcgacgc agcccatggc ctcggcccac ttcacgtagt cgggcaggtc
     1381 cggcgacagg tacacctcgg agtaccgctc gtcgtagaac atctcctgcc actggcggac
     1441 catgccgagg taggcgttgt tcaagatggc gatcttgacg gggatgcgct cggcggccgc
     1501 cgtgaccagc tcctgggcgg tcatctggaa gcagccgtcg ccgtcgatgg cccagaccat
     1561 gcggtcgggc cggccgacct tggcgccgat ggcggccggc acggagaagc ccatggtgcc
     1621 gagcccgccc gagttgatcc aggtgtaggg gtggcggaac gtccagtact ggctggccca
     1681 catctggtgc tggccgacgc cgctggcgac gatgcagtcc tcgggggccg cgtcccggag
     1741 cgcttcgagc accatctgcg gcttgagcag ctcgccttcc accgggtcct cgtaggccag
     1801 cgggtacgtc tcctgccagc ccgacagggt ggagcgccac ggggtgatgt cgggcttgac
     1861 cgcacccttc gccagctggg ccctggcctc ggcgacgagc gcctcgatca ccagacggga
     1921 gtcgccgacg atcggcacgt cgggccggcg gaccttgccc agctcggccg ggtcgatgtc
     1981 ggcgtggatg atcttggcgt gcggggcgaa gttcgggacc ttgccggtga cgcggtcgtc
     2041 gaagcgcgag cccaaggcga tgagcaggtc gctctcttgc atcgacatga tcgccgtgta
     2101 gttgccgtgc atgcccggca tgccgaggca cagctcgtgg tcgtcgggga acgcaccccg
     2161 ggccatgagc gtggtgacga cggggaactc gaacagctcg gccagctcga ggagcgcctc
     2221 ggcggcgcgg cccttgagga tgccgccgcc cgcgtagatg accggccgct cggaggccag
     2281 catcagctcg acggcctcct tgatcttctt cgggtgcccg gtggtggtcg gcttgtagcc
     2341 ggggaggctg gcggtgacct cgtcgtcgct cggccacacc cactccatgg cgctgcgcgg
     2401 gttggtgggg tcgacgatgt ccttggggat gtcgacgagg accgggccgg gccggccggt
     2461 ggtggcgatg tggaacgcct ggcggatcgc catcggcacg tcctgcgcct cggtcacgag
     2521 gaagttgtgc ttggtgaccg actgggtgat gcccgtgatg tcggcctcct ggaacgcatc
     2581 ggtgccgatg gcggcggtgg ccacctggcc ggtgatgcag accatgggca cggagtccat
     2641 gtaggcgtcg gcgaggggcg tcacgatgtt ggtggcgccc ggaccgctgg tcaccatggc
     2701 cacgccgggc ttgccggtgg cgtgggcgta gccctcggcc atgtggccgg cgccctgctc
     2761 gtggcggacg aggatgtggc ggatcggcga gtcgatgatc gggtcgtaca ccgggaggat
     2821 ggccccgccg ggcaggccga agatgacctc gaccccttcc atctccaggc tcttgatgag
     2881 cgcctggcct ccgttgagct tcatggtgtt ccctctggga ttcgtgcagc ggacgtgcgg
     2941 gtgagcgggt gggcggtgcg cacgctccct gcagtctgcc cggtgccgaa cccgacccga
     3001 aaactgcgac gacctcccgg tctgggaggt cgaggaagcg cacgcggcgg gtgaagacgg
     3061 cgtgcgctac gtgagtacta cgaggcggta ggcggtcttc agcacgaggg cagtatgacg
     3121 gagcccccag catctggcaa cccggttccc ccgggtcagt cgatcaaccc acggggcacg
     3181 acccagcggc tgggctgccc ggtgacctcg gcgatgaggt cgaagtcagc gtcgtagtgc
     3241 acgaccggga gatcggccgc ctctgccgcg gcggcgatga ggagatccgc catcgatacg
     3301 ccccggtgac ggccctgcgc gcaaagcgca ccttgcacct cgatcgcacg gtcgaacact
     3361 gcctgatggg tacggacctg agggagggca tcgaactggc gacgaaggcg ctcgtaggct
     3421 gcgccgttct gtgcgctgaa gcacacctcg aagatgacgg gagcgcagac ggcgacgtcg
     3481 cccgcttcga ccgcgtcttc gatctcgatc gcgaccagat cgctggcaaa gcggttgacg
     3541 acgctcgtat cgaccaggac ccgggtcatc tcgaggcgcg gttcatcagc tcccgctcct
     3601 ccggggtgaa cggatggtcc cggaaccact ggaaggccgc cttgcgctcc gcccgggcca
     3661 ccacctcgcg cagcgcttct tccaccgtcg cggtcagggt ttcggtaccg aggagctcgc
     3721 ctgcccggtc cgccagatcc cgatcgatgt tgatcgctgt cttcgccatg ccgaccaagc
     3781 tatcaccgag acctctccat ggctatcccg atgggcaaaa acacatggaa gttgcgaaag
     3841 agcatgcggg agggccagtt cgatcggatg aggaacagct ggagcggacc gccggaggac
     3901 gcgcgtacac gccgaagggc gtgacgcggt acgtgacgat cgccccgctg gcggtcttca
     3961 ccgccctggt gaggtcggga tgctctggtt gatgatccct gagcccgccc ctcaggctgg
     4021 ctcggaccgg ctacttggtg ttggtgatgg cgccggtctc ggcgccttgg accaggcggg
     4081 cgtacttgcc caagacgcct tgggtgtagc gcggcgggtt cggcgtccag ccgaccttgc
     4141 gggcctcgag ctcgacctcg tcgaccatca ggtccaggcg gaactggtgg acgtcgatga
     4201 cgatgcggtc gccgtcgcgc acgaaggcga tgggaccgcc gtcgacggcc tcgggggcga
     4261 cgtggccgat gcagaagccc caggtgccgc cggagaagcg gccgtcggtg atgagggcgc
     4321 agtccgagcc gcggccggca cccttcagcg caccggtgat ggcgagcatc tcgcgcatgc
     4381 cggggccgcc cttggggccc tcgtagcgga tgacgatcac ggtgccgggc gcgatgtcgc
//
LOCUS       DATMFS010000454         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_15691, whole genome shotgun sequence.
ACCESSION   DATMFS010000454 DATMFS010000000
VERSION     DATMFS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15691"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VNQ33_08575"
     CDS             complement(<1..812)
                     /locus_tag="VNQ33_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ98199.1"
                     /translation="MADRLVVIGGDAAGMSAATNARRGRPDLEIVVLEQGAHTSYSAC
                     GIPSVVSGSVGALDELVVRTPQEFRDGFRIDVRTRHEVKAIDLDARRIEVRAIDQART
                     LHLGFDLLHLATGSRPMRPDLPGIDGPDIYGVQTLGDAEVLLEAIRSRPRRAVTVVGA
                     GSIGLEMAEAFVRRGARVTLVDSNPLPMRTFDPDMGARIAAAVRRFGIELRTGTRVEG
                     FEPGRVHTGTGAIDADLVILGLGVTPNSAIGIDAGLDAGAAGAIAVDHRQRT"
     gene            890..1078
                     /locus_tag="VNQ33_08580"
     CDS             890..1078
                     /locus_tag="VNQ33_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98200.1"
                     /translation="MAVRQDCRHYSTRTVASGEQVQRCRVDANDKAPFACPEFCLFFE
                     PRSITDAGWQRFEGDEDR"
     gene            1075..1548
                     /locus_tag="VNQ33_08585"
     CDS             1075..1548
                     /locus_tag="VNQ33_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWJ98201.1"
                     /translation="MRITAKVDYAVRAAIELARAYPTDGARPTPLTRQAIAEAQDIPS
                     KFLEHILADLKRSQIVGSVRGADGGYWLHRPPAEITMADIIRAAEGPLADVRGERPDA
                     LDYPDDLAVLQRAWIALRANLRAVLEQVTLADLRDGKLLPDVDRLADSDDAWVIR"
     gene            1578..1928
                     /locus_tag="VNQ33_08590"
     CDS             1578..1928
                     /locus_tag="VNQ33_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98202.1"
                     /translation="MPSEPGAAPPVALHEADPGLPGQRHLGIGLDVDPDGQRPERRGA
                     TSQGTDGGDVGRRAEVESGQADRDGEAHPERGDGHRGTAAHAQGELGLGDPQDEPLGR
                     QAGAAQQRLHIGHR"
BASE COUNT          341 a          857 c          837 g          283 t
ORIGIN      
        1 ctcgtccgct ggcggtggtc gacggcgatg gcgccggccg ccccggcatc gaggccggcg
       61 tcgatgccga tggccgagtt gggcgtgacg cccaggccga ggatgaccag gtcggcgtcg
      121 atggcgcccg taccggtgtg gacgcggccc ggctcgaacc cctcgaccct ggtaccggtg
      181 cgcagctcga tgccgaaccg gcgcaccgcc gcggcgatcc gcgcccccat gtccgggtcg
      241 aacgtccgca tgggcagcgg gttgctgtcg acgagggtga cgcgagcgcc gcgccggacg
      301 aacgcctcgg ccatctccag gccgatggag ccggccccca cgaccgtgac cgcacgcctc
      361 ggccggctgc ggatggcctc gaggaggacc tcggcgtcac cgagcgtctg gacgccgtag
      421 atgtccggac cgtcgatgcc ggggaggtcg ggacgcatcg gccgggaccc ggtggcgagg
      481 tggagcaggt cgaagcccag gtggagggtc cgcgcctggt cgatggctcg gacctcgatc
      541 cgccgggcgt cgagatcgat ggccttcacc tcgtggcggg tgcgcacgtc gatgcggaac
      601 ccgtcgcgga actcctgggg cgtccggacg acgagctcgt cgagcgcccc caccgagccg
      661 gagacgacgg aggggatccc gcacgccgaa taggaggtgt gggcgccctg ctcgagcacc
      721 acgatctcca gatcgggccg tccccggcgc gcgttggtgg cggccgacat gccggccgca
      781 tcgcctccga tgaccaccaa ccggtctgcc atggcgcgca gactaccgat ggtctgccca
      841 gaccaggggc tattccccgc tgttcgacca gaaatgcgaa actgcgtgca tggccgtgcg
      901 tcaagactgt cgccactact cgacgcgcac cgtcgcgtcc ggggagcagg tacagcgctg
      961 ccgcgtggac gccaacgaca aggctccgtt cgcctgcccg gagttctgcc tcttcttcga
     1021 accccggtcg atcaccgacg ccggctggca gcgcttcgaa ggggatgagg atcgatgagg
     1081 atcaccgcga aggtcgacta cgccgtccgg gccgccatcg agctggcccg cgcctacccg
     1141 accgacgggg cccggccgac accgctcacc cggcaggcga tcgccgaggc ccaggacatc
     1201 ccgtcgaagt tcctcgagca catcctcgcc gacttgaagc gcagccagat cgtgggcagc
     1261 gtgcgcggcg ccgacggcgg ctactggctc caccgcccac cggccgagat cacgatggcc
     1321 gacatcatcc gcgccgccga gggcccgctg gccgacgtgc gcggggagcg ccccgacgcc
     1381 ctggactacc cggacgacct cgccgtgctg cagcgggcgt ggatcgccct gcgggccaac
     1441 ctccgggcgg tgctcgaaca ggtgacgctg gccgacctgc gcgacgggaa gctgctcccc
     1501 gacgtggacc ggttggccga ctccgacgat gcctgggtca tccggtgatc ggctggcggg
     1561 cccacgtgtg gacgagcttg cccagcgagc ccggggctgc cccaccggta gccctgcacg
     1621 aggccgatcc cggcctcccc ggccagcgcc acctcggcat cggactcgac gtcgaccccg
     1681 atggccagcg ccccgagcga cgcggcgcga cctcgcaagg cacggacggc ggcgacgtcg
     1741 gccggcgcgc cgaggtcgag tccggccagg ctgatcgcga cggcgaagcg caccccgagc
     1801 gcggcgatgg gcaccgcggc accgctgctc acgcccaggg cgagctcggc ctcggtgacc
     1861 cgcaggacga gccgctcggg cgccaggccg gtgccgcaca gcagcgcctc cacatcggcc
     1921 accgctgacg acgacacggc cgctccgtgg tgcagggcca gtgccagcca ccagtcctcc
     1981 aggaccggca cctcggtggc gaacgtccgg acggtgaccg gtgccggagc ggtggtgcgc
     2041 agcagctcgg caccgaccac ccggccggtc tcgatcgcca gcaccggacg gtaccggggc
     2101 tccccggtcg ccacgggcgc gtcgatggtg gtcctcccgc tcacagatgg gggaaggcct
     2161 cgggcaccgc ggatctgacg ccgcccgcgc gatcggggga acccccagcg cgatcggggg
     2221 agccgtcagc gcaggatcaa gcggaggtcg accaggtcga tgggcgtctc ggtcacgtcg
     2281 accgagccga cgatggtggc ccaggtgctc ccgccgac
//
LOCUS       DATMFS010000455         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_39432, whole genome shotgun sequence.
ACCESSION   DATMFS010000455 DATMFS010000000
VERSION     DATMFS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39432"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VNQ33_08595"
     CDS             <1..1167
                     /locus_tag="VNQ33_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98203.1"
                     /translation="LTMLGILIGITAVILTVGLGTGAKAEVRDQIDELGTNLLVVSPG
                     SSTDTSGIRGGFGSSSTLTIEDAEAIADPKAAPDVQAVAATATTSVSLTNGDTNWTTT
                     LTGTTPSWAEVRSRDVTSGRFLTEEDEEDAAAVVVLGPDTASELFTAGNPVGQYVTGN
                     GAKLKVVGVLEELSSSEDTSSNDLAIVPSSTYSERLIGGSSRDSVSSIYVKATSEDTL
                     SAAYQEANALLLNLHGITSADDADFSIATQESILTAATSVDKTMTVMLGGIAVISLLV
                     GGIGVMNIMLVSVTERIREIGLRKALGARPRSIRRQFLVEASVLGFAGGVLGVLLGVV
                     GAVVLPHLTGTRVILSLTASVAAIAMAIGIGVVFGVYPASRAARLAPIDALRSE"
     gene            1169..>2537
                     /locus_tag="VNQ33_08600"
     CDS             1169..>2537
                     /locus_tag="VNQ33_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWJ98204.1"
                     /translation="MPSFFTRRRLAAASVVVVVIGGAVAAASARSESSPSYRTTRAGM
                     EDVDQELHTVGSVEPVSQASVSFPVAGTVAGVDVAVGEEVTVGSRLAALDVADLTVTL
                     HEKQAALDQASLVLQMALDGEDVSGLASGGGPANGTFGRPAAGGANAELKAAQQAVLH
                     AQKAVDEAAAAAGLAYANAVSVCRPATVPTAIEEGSTTTTTTTATDSSQACLDALQAV
                     LGAQAQVAAAQAQLTDAVDALRTLLDETANRPSTPTTTTSSPPSIPSGGSSSNDGGSS
                     PSGSPGSNGSSGLPSGGTGASSSPSAEDLVAHQKAVDAAEDEVAVAQQAIDQATVVSP
                     IDGRIASVNLQVGDEVEAASSTADIIVVGEGGFDVTTSVSVKDLPDLEVGQAATVKPD
                     GVDAAVGGEVVGIGVTADATGSSYPVTIGLTGDTSAMGNGSTASVVIVTSAAEQALAV
                     ATSAVT"
BASE COUNT          357 a          957 c          873 g          350 t
ORIGIN      
        1 ctgacgatgc tcggcatcct gatcggcatc accgccgtga tcctcacggt cggcctcggc
       61 accggggcca aggccgaggt gcgggaccag atcgacgagc tcggcaccaa cctcctggtg
      121 gtctcgccgg gcagctccac cgacacctcc ggcatccggg gcggcttcgg ctcgtcatcg
      181 accctgacca tcgaggacgc cgaggcgatc gccgatccga aggcggcgcc cgacgtgcag
      241 gctgtcgcgg ccaccgcgac cacctcggtc tcgctcacca acggcgacac caactggacc
      301 accacgctca ccggtaccac gccgtcgtgg gccgaggtcc gctctcgtga cgtgacgtcg
      361 ggtcgcttcc tcaccgagga ggacgaggag gatgccgcgg ccgtggtggt gctcgggccc
      421 gacacggcat cggagctgtt caccgccggc aacccggtcg gccagtacgt caccggcaac
      481 ggggcgaagc tgaaggtcgt cggcgtgctc gaggagctgt cgtcgtcgga ggacacgtcc
      541 agcaacgacc tggcgatcgt gccgtcctcc acctactcgg agcggctgat cggcggctcc
      601 tcccgggact ccgtcagctc gatctacgtg aaggccacgt cggaggacac cctctccgcc
      661 gcctaccagg aggccaacgc cctcctgctg aacctgcacg gcatcacgtc ggccgacgac
      721 gccgacttct cgatcgccac gcaggagtcg atcctcacgg ccgccacctc ggtcgacaag
      781 acgatgacgg tgatgctcgg cggcatcgcc gtgatctcgc tgctcgtcgg cgggatcggc
      841 gtgatgaaca tcatgcttgt ctcggtcacc gagcggatcc gggagatcgg cttgcgcaag
      901 gcgctcggcg cccggcctcg gagcatccgc cgccagttcc tggtggaggc gtccgtgctc
      961 ggcttcgccg gcggcgtgct cggggtcctg ttgggcgtcg tcggcgccgt cgtgctcccg
     1021 cacctgaccg gcacccgcgt gatcctctcg ttgaccgcgt cggtcgccgc catcgccatg
     1081 gcgatcggca tcggcgtggt gttcggggtc taccccgcat cccgggccgc ccgcctggcc
     1141 cccatcgacg ccctccggag cgagtgacat gcccagcttc ttcaccagga ggcgactggc
     1201 cgcggccagc gtcgtggtgg tcgtcatcgg cggtgccgtg gccgcggcga gcgcccggtc
     1261 cgagtccagc ccgtcgtacc gcaccaccag ggcgggcatg gaggacgtcg accaggagct
     1321 gcacaccgtc ggctcggtcg agccggtgtc ccaggcgagc gtctcgttcc ccgtggccgg
     1381 caccgtggcc ggcgtcgacg tcgccgtcgg cgaggaggtc accgtgggct cgcgcctggc
     1441 cgcgctcgac gtggccgacc tgacggtgac cctgcacgag aagcaggccg cgctcgacca
     1501 ggccagcctg gtcctccaga tggccctcga cggcgaggac gtctccggcc tggcctccgg
     1561 cggcggtccg gcgaacggca ccttcggccg tcctgccgcc ggcggtgcga acgccgagct
     1621 gaaggcggcc cagcaggccg tgctccacgc ccagaaggcg gtcgacgagg ccgctgccgc
     1681 cgccggcctg gcctacgcca acgcggtgtc ggtgtgccgg ccggccacgg tgccgaccgc
     1741 catcgaggag gggtcgacca ccaccaccac caccacggca accgacagca gccaggcgtg
     1801 cctcgacgca ctccaggccg tcctcggcgc ccaggcccag gtcgccgcgg cccaggccca
     1861 gctgaccgac gccgtggatg cgctgcgcac cctgctcgac gagacggcga accgcccgtc
     1921 gacgcccacg accaccacct cctcgccgcc gtcgatcccg agcggtggca gcagctcgaa
     1981 cgacggcggc tcgtcgccaa gcggctcgcc aggttcgaac gggtcgagcg gcctgccgtc
     2041 cggtggcacg ggtgcgtcct cgtcgccctc ggccgaggac ctggtcgcgc accagaaggc
     2101 cgtcgacgcc gccgaggacg aggtcgcggt ggcccagcag gccatcgacc aggccaccgt
     2161 cgtcagtccc atcgacggcc ggatcgcctc ggtgaacctg caggtcggcg acgaggtcga
     2221 ggcggcttcg tccaccgccg acatcatcgt ggtcggcgag ggcggcttcg atgtgacgac
     2281 gtcggtcagc gtcaaggacc tgccggacct cgaggtcggg caggccgcca cggtcaagcc
     2341 cgatggcgtc gacgccgcgg tcggcggcga ggtcgtcggc atcggcgtca cggccgacgc
     2401 cacgggatcg agctacccgg tcaccatcgg gctgaccggt gacaccagcg ccatgggcaa
     2461 cggatccacc gcctcggtcg tcatcgtcac ctcggcggcc gagcaggcgc tcgccgtcgc
     2521 gacctccgcg gtcaccg
//
LOCUS       DATMFS010000456         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_176680, whole genome shotgun sequence.
ACCESSION   DATMFS010000456 DATMFS010000000
VERSION     DATMFS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176680"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..812)
                     /locus_tag="VNQ33_08605"
     CDS             complement(33..812)
                     /locus_tag="VNQ33_08605"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98205.1"
                     /translation="MSAAGRGAVDVRGLTVAWPGADGMKAAVEDLDLQVESGSFAALV
                     GPSGCGKSTVLRVLAGLLAPDGGSVAVDGADVAGRPGATAWLPQRDALLPWRRVLGNA
                     VLGAEMHGVPRAEAEATARALLDRFGLGGTEQQWPSELSGGMRQRVAVLRAFLTPAPV
                     LLLDEPFGALDALTRRQMQSWLQEVLADETAGGRRTVVLVTHDVEEALLLSDRVLVSS
                     PAPGRIVADVPVAFARPRMADLVADPAFVRQRAGLLAALGA"
     gene            complement(809..1831)
                     /locus_tag="VNQ33_08610"
     CDS             complement(809..1831)
                     /locus_tag="VNQ33_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98206.1"
                     /translation="MIRRPVLLLVALAAVALPLAACGGSATDGGSSGGGGSGHLRPFK
                     VVLDWTPNTNHAGMYLAKANGWYSDAGLDVTFIEPGEAGSLQALAAGKADVAVSVQEE
                     LIPARAAGLPVRSIAAIIQHNTSSLVSLAGDGITRPKDLEGKTYGGYGGQLEQALLDK
                     LVACDGGDPKKVRSVDVGEADYRIGLERNQYDAVWIFDGWDGIRLKQEGVDTSTIPFI
                     DHEDCIPDWYTPLLAASEQVEQERGDDLTAFMAATAKGYQAAMDDPDAASDALLDQAD
                     DLDPELVRASAAYLSPRYADDPARWGEQEASVWDGFAAFLLDAGLIDKPIEVGDAWTN
                     EYLPAS"
     gene            complement(1828..>2044)
                     /locus_tag="VNQ33_08615"
     CDS             complement(1828..>2044)
                     /locus_tag="VNQ33_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98207.1"
                     /translation="GGSSTDKGLGKTILRSLASYRFDQVFVAVVLVAVLSGLLFVLVD
                     QLGRLAVPWEQTRRRTARHHPPSKEIP"
BASE COUNT          278 a          758 c          747 g          261 t
ORIGIN      
        1 gtgcgccgca cccgccgaaa ccgaccgacg ccctacgcgc ccagcgcggc gagcagcccg
       61 gcgcgctggc ggacgaacgc ggggtcggcg acgaggtcgg ccatccgggg ccgggcgaag
      121 gccaccggca cgtcggcgac gatgcgaccc ggtgccggcg acgagacgag cacccggtcc
      181 gacagcaaga gcgcctcttc gacgtcgtgg gtcaccagga ccacggtgcg ccggccgccg
      241 gcggtctcgt cggcgagcac ctcttggagc cacgactgca tctgacggcg ggtcagcgcg
      301 tcgagcgcgc cgaacggctc gtcgagcagg aggacgggtg cgggcgtgag gaaggcccgg
      361 agcacggcca cgcgctggcg catgcccccc gacagctccg acggccactg ctgctcggtg
      421 ccgcccagcc cgaagcggtc caggagcgcc cgtgccgtcg cctcggcctc ggcccgcggc
      481 acaccgtgca tctcagcgcc cagcacggcg ttgccgagga cgcgccgcca cggcagcagg
      541 gcatcgcgct ggggcagcca ggccgtggcc ccggggcgcc cggcgacgtc ggcgccgtcc
      601 accgcgaccg agccgccgtc gggtgccagg agcccggcga gcacgcgcag gaccgtgctc
      661 ttgccgcacc cgctgggccc gacgagggcc gcgaacgagc ccgactcgac ctggaggtcg
      721 aggtcctcga cggccgcctt catcccgtcc gcgcccggcc aggcgacggt caagccgcgg
      781 acgtcgaccg cgccgcggcc ggcggcgctc acgacgccgg caggtactcg ttcgtccacg
      841 cgtccccgac ctcgatgggc ttgtcgatca ggcccgcatc cagcaggaag gccgcgaagc
      901 catcccacac cgacgcctcc tgctctcccc accgtgcggg gtcgtcggcg tagcgggggc
      961 tgaggtacgc ggcgctggcc ctcaccagct cgggatcgag gtcgtcggcc tggtccagga
     1021 gggcgtccga cgccgcatcc ggatcgtcca tcgccgcctg gtagcccttc gccgtggccg
     1081 ccatgaacgc ggtgaggtcg tcgccccgct cctgttcgac ctgctccgac gcggccagga
     1141 gcggcgtgta ccagtcgggg atgcagtcct cgtggtcgat gaaggggatg gtgctcgtgt
     1201 cgacgccctc ctgcttcagg cggatgccgt cccagccgtc gaagatccac accgcgtcgt
     1261 actggttccg ttcgaggccg atccggtagt cggcctcgcc cacgtcgacg gaccgcacct
     1321 tcttcgggtc gccgccgtcg caggccacca gcttgtccag cagggcctgc tcgagctggc
     1381 cgccgtagcc gccgtaggtc ttgccctcga ggtccttcgg gcgcgtgatc ccgtcgccgg
     1441 ccagcgacac caggctcgag gtgttgtgct ggatgatcgc ggcgatcgac cgcaccggga
     1501 ggccggcggc gcgagcgggg atcagctcct cctgcacgga cacggcgacg tcggccttcc
     1561 cggccgcgag ggcctggagg ctgcccgcct cccccggctc gatgaacgtc acgtcgagcc
     1621 cggcgtcgga gtaccagccg ttcgccttgg ccaggtacat gccggcgtgg ttggtgttcg
     1681 gtgtccagtc gaggaccacc ttgaacgggc gcaggtggcc ggacccgcca ccgccgctgc
     1741 tccctccatc ggtcgcgctg cccccgcacg ccgcgagcgg cagcgccacc gccgcgaggg
     1801 cgacgagcag gaggacgggt cggcggatca tgggatctcc ttggaagggg gatggtgtcg
     1861 agcggtgcgc cggcgggtct gctcccacgg cacggcgagg cggccgagct ggtcgaccag
     1921 gacgaagagg aggccgctca gcacggcgac gaggacgacg gccacgaaga cctggtcgaa
     1981 ccggtaggac gcgagcgagc ggaggatcgt cttgccgagc cccttgtcgg tgctcgaccc
     2041 gccg
//
LOCUS       DATMFS010000457         3242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_341802, whole genome shotgun sequence.
ACCESSION   DATMFS010000457 DATMFS010000000
VERSION     DATMFS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341802"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VNQ33_08620"
     CDS             <1..1318
                     /locus_tag="VNQ33_08620"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HWJ98208.1"
                     /translation="VDPAWRSGYYEVVLTVDVDGKQRRSHAFFVVRPPTGAPTAPALL
                     ALSTNTWNAYNDFGGNQLYTGATSVSFQRPMAAGYLHKPPGEGRRVTNQAPYDPQMTA
                     HRGYLAAHHLSPFAGSAGWPDWELPFLQWAEREGFAVDVVLNADLAEHPGLLAQTAAE
                     GGYSLYLSVGHDEYWSGHMRDTVEGFIARGGNAAFLSGNTSFWQVRLEDPTPEGPAAT
                     MVGYKGFFKSDPVYGTDRIGELTAMWSDKEIGRPENQMTGVSFTRGGYHRIGKRATRG
                     AGGYTIHRADHWLFEGTGLDYGDVLGGESRTVGYECDGCDFTYRNGLPYPTGEDGTPP
                     DFEILGTVPAAHFTRTTATRPPPPDQPSEVEFIAARIAGGGRTPEHVARVEHGHAVLG
                     TYTSSGGGIVVTSGSTDWAHGLGGTDPTVEQVTRNLLTRLAGPPSR"
     gene            complement(1418..1774)
                     /locus_tag="VNQ33_08625"
     CDS             complement(1418..1774)
                     /locus_tag="VNQ33_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98209.1"
                     /translation="MADEGDPGHDASRGGRRRSLDPQGRRALFETPVSAARDTIRSGT
                     PRDGKDALYSTGPRQTGTVIVTCSACESRSRINLTDLGLRWMGGTIWVPGRKNSHWMR
                     CPSCAKRTWCAIGWQD"
     gene            complement(1774..2565)
                     /locus_tag="VNQ33_08630"
     CDS             complement(1774..2565)
                     /locus_tag="VNQ33_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ98210.1"
                     /translation="MTRVVAIANQKGGVAKTTSTHALGVAMSERGRKILLVDLDPQAS
                     LTWACGVDPDLLEVSIHDVLLKRAKAEDAIVALPAPKPAKEGKPGVLHLLPSTIDLAG
                     AELNLLTKTGREYVLRKALSKVSDRYDRVLVDCPPSLGILTINGLTAADEVVIPLQCE
                     SLSQRGVGQLLETIEDVRDYTNDDLEVVGAIATLFDGRTKLAHEVVEQVRRTYDIRVL
                     EPFVPKSVKVAEAPARGKSVLQHAPKSRSAQAYRELANQLLGRKG"
     gene            2608..3159
                     /gene="pyrE"
                     /locus_tag="VNQ33_08635"
     CDS             2608..3159
                     /gene="pyrE"
                     /locus_tag="VNQ33_08635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWJ98211.1"
                     /translation="MTKAALIDHLLAHSVKRGDFVLKSGKATSWFIDAKQTTCSPDGM
                     LLVADAALEAMPDGITAVGGLTMGADAMAFSIAAVAATRGRQLRAFSVRKEVKDHGGG
                     GRIAGALEPGDKVLITEDAVTRGVSMLEAAEVVREAGAEPVLLMPVVDRGGTVTAMAA
                     EQGLPVLALVTAEDLGFPFEGPV"
BASE COUNT          452 a         1184 c         1134 g          472 t
ORIGIN      
        1 cgtcgatccg gcgtggcgca gcgggtacta cgaggtcgtc ctcaccgtcg atgtcgacgg
       61 gaagcagcgc cgcagccacg ccttcttcgt ggtgcggccg cccactggtg caccgaccgc
      121 gccggcgctg ctggcgctgt cgaccaacac gtggaacgcg tacaacgact tcggcggcaa
      181 ccagctctac accggcgcca cctcggtctc gttccagcgg cccatggcgg ccggctacct
      241 gcacaagccg cccggtgagg ggcggcgggt cacgaaccag gcgccctacg acccgcagat
      301 gaccgcccat cggggctacc tggcggctca ccacctctcg cccttcgcgg gttcggcagg
      361 ctggccggac tgggagctgc cgttcctcca gtgggccgag cgcgagggct tcgccgtcga
      421 cgtcgtcctc aacgccgacc tggccgagca ccccggactc ctcgcccaga ccgcagctga
      481 aggcggctac tcgctgtacc tctccgtggg ccacgacgag tactggtcgg gccacatgcg
      541 cgacaccgtc gagggcttca tcgcccgcgg cggcaacgcc gcgttcctct cgggcaacac
      601 ctcgttctgg caggtgcggc tcgaggaccc gaccccggag gggccagcgg cgacgatggt
      661 cggctacaag ggcttcttca agtccgaccc ggtgtacggc accgaccgca tcggcgagct
      721 caccgccatg tggtccgaca aggagatcgg ccggcccgag aaccagatga ccggcgtgag
      781 cttcacccgc ggcggctacc accgcatcgg caagcgggcc acccgcggcg ccggcggcta
      841 cacgatccac cgggcggacc actggctgtt cgaggggacc gggctggact acggcgacgt
      901 cctcggcggc gagagccgga ccgtcggcta cgagtgcgac ggctgtgact tcacctaccg
      961 caacgggttg ccctacccga cgggcgagga cggcacgccg cccgacttcg agatcctcgg
     1021 cacggtcccg gcggcgcact tcacccggac gactgccacc cgcccgccgc caccggacca
     1081 gccgtccgag gtcgagttca tcgccgcccg catcgccggc ggcggccgga cccccgagca
     1141 cgtcgcccgg gtcgagcacg gccacgccgt cctcggcacc tacacctcgt ctggcggcgg
     1201 catcgtcgtc acttccggga gcaccgactg ggctcacggc ctcggcggca ccgatcccac
     1261 cgtcgagcag gtcacccgca acctcctcac ccgcctcgcc ggcccgccca gccggtgacc
     1321 accgatttcg ggtcacacgg gcactgatgg ggtgcgcgcg tgacctgaaa tgcgtgatgg
     1381 gcctcgggcg agcggctgcg gccggctcag gagtcgttca gtcctgccag ccgatggcgc
     1441 accaggtgcg cttcgcgcag ctcgggcagc gcatccagtg gctgttcttg cggccgggaa
     1501 cccagatggt gccgcccatc cagcggaggc cgaggtcggt gaggttgatg cggctgcgcg
     1561 actcgcaggc cgagcacgtg acgatgaccg tgccggtctg gcgggggccc gtggagtaga
     1621 gggcgtcctt gccgtcgcgc ggcgtcccgg agcggatggt gtcgcgtgct gcgctcaccg
     1681 gggtctcgaa cagcgcccgg cggccctgcg ggtcgagcga ccggcgccgg ccgccgcggc
     1741 tggcgtcgtg gccgggatcg ccctcgtcgg ccatcagccc ttgcgcccca gcagctggtt
     1801 ggccagctcg cggtaggctt gggcgctccg ggacttcggt gcgtgctgga gcaccgactt
     1861 cccgcgggcc ggcgcctcgg cgaccttgac cgacttcggg acgaacggct ccagcacccg
     1921 gatgtcgtag gtgcggcgga cctgctcgac cacctcgtgg gccagcttgg tccggccgtc
     1981 gaagagcgtg gcgatcgcgc ccaccacctc gaggtcgtcg ttcgtgtagt cgcggacgtc
     2041 ctcgatggtc tcgaggagct ggccaacacc gcgctggctg agcgactcgc actgcagcgg
     2101 gatgacgacc tcgtcggccg ccgtgaggcc gttgatcgtg aggatgccga gggagggcgg
     2161 gcagtcgacg agcacccggt cgtagcggtc cgacaccttc gacagcgcct tgcgcagcac
     2221 gtactcccgg ccggtcttgg tgagcaggtt gagctcggcg cccgcgaggt cgatggtcga
     2281 cggcagcagg tgcagcaccc cgggcttgcc ctccttcgcc ggcttgggcg cgggcagggc
     2341 gacgatggcg tcttcggcct tggcccgctt caacaagacg tcgtggatcg acacctcgag
     2401 cagatccggg tccaccccac acgcccacgt gagcgacgct tgcgggtcga ggtcgacgag
     2461 gaggatcttg cggccccgct ccgacatggc gacgcccagc gcgtgggtcg aggtggtctt
     2521 ggccacgccg cccttctggt tcgcgatcgc caccacccgc gtcatggcga cgagtgtggc
     2581 acccctagct cggtagggtc gacttccatg accaaggctg cgctcatcga ccacttgctc
     2641 gcccactcgg tcaagcgggg cgacttcgtc ctcaagtcgg gcaaggccac ctcgtggttc
     2701 atcgatgcca agcagacgac ctgcagcccc gatgggatgc tcctcgtggc tgacgccgcg
     2761 ctggaggcga tgcccgacgg catcaccgcg gtcggcggcc tcaccatggg cgccgacgcc
     2821 atggcgttct cgatcgccgc ggtcgcggcc acgcgaggcc gccagctgcg ggcgttcagc
     2881 gtccgcaagg aggtcaagga ccacggcggc ggcggccgca tcgccggtgc gctggagccg
     2941 ggggacaagg tcctgatcac cgaggacgcc gtgacccgcg gcgtgtcgat gctcgaagcg
     3001 gccgaggtgg tccgcgaggc aggcgccgag ccggtgctgc tcatgccggt ggtggaccgc
     3061 ggcggcaccg tcaccgcgat ggcggccgag cagggcctgc ccgtcctcgc cctcgtgacc
     3121 gccgaagatc tgggcttccc gttcgaaggc cccgtctgat ccgctccgga ccccctcctg
     3181 ccagctgacg gcggatcacc ccgcggcgcg gcgcccttgg taaggtgcgc tgcacatcga
     3241 cc
//
LOCUS       DATMFS010000458         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_15833, whole genome shotgun sequence.
ACCESSION   DATMFS010000458 DATMFS010000000
VERSION     DATMFS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15833"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="ftsW"
                     /locus_tag="VNQ33_08640"
     CDS             <1..990
                     /gene="ftsW"
                     /locus_tag="VNQ33_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWJ98212.1"
                     /translation="ALWTVLGTVALFATLMVSVDFWRRFTKPFMAIAIAMLVLVLVPG
                     IGVTVNGATRWLGAGPFQIQPSEFAKFALLLFVADLLDRRSDKTADWRWTLAPVVAYL
                     GLIAALIMLQPNLGTTIIIAFMVLTMLLVAGVPLLPLSAVAAAGAMVATAFVAYTPFR
                     RTRFLRFLNPWADPQNTGLQNVQSLVGIANGGLLGRGLGRSTAKWGFLPFAHTDFIFA
                     VIGEEFGLVGAMSVVLLFFGITCTGAWIAARAKDRFSMLVATGITAWIGIQAFMNIGA
                     VIGIMPITGVPLPFISFGGSSLMINLAAAGMLLNIARHPAEHLPRSSRRAKRS"
     gene            987..2099
                     /locus_tag="VNQ33_08645"
     CDS             987..2099
                     /locus_tag="VNQ33_08645"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HWJ98213.1"
                     /translation="MSEPTWALVAGGGTAGHLLPGLSVARALVAEGRDPATIHFVGAE
                     RGPEQELVPAAGFTVDVLPGRGIQRKLSAANVAAVLDLFKALFRGIGIVRTRKPSVVI
                     VLGGYASFACGVGAVFGRVPLVLLEQNKKAGAVNRALRRFAAASAVSFEGTDLPKATV
                     TGNPLRPEIRQMAEHPDPAAARAALDLPADRTVIGVFAGSLGSRRINDAVQGVVERWA
                     HRSDLAVRHVVGRNGFSDGSDGPTGLPADGIVYQRVPYEDRMHLLLDASDLAVTRSGG
                     SVFELMAAGLPAVLVPLPIATRDHQMANARAVEALGGAVVVPDSELDTDRLEAELTAL
                     LGDRERLAAMAAALRAGAAIDAAEQAAAVVERVARG"
     gene            2092..3522
                     /gene="murC"
                     /locus_tag="VNQ33_08650"
     CDS             2092..3522
                     /gene="murC"
                     /locus_tag="VNQ33_08650"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWJ98214.1"
                     /translation="MAEPVTGLSAPRTVHLVGIGGAGISAIGIILATMGHRVTGSDVN
                     ETSAWPSLIDAGVHPEVVAPGGLFASAHAHGAEIVAHSTAFPPTERDLEAAAAAGCTV
                     LDRAGILALICAARPTVAVSGTHGKTSTTAMLATLLDGAGTDPSYLVGAKPVGLGRAA
                     HWAGGGSGAFVVEADESDGTFLRLGGDIAVVTNIDEDHLDYWRDIDAIEAAFLRFLTE
                     ARVGVVGIDDPRGEHITDPRALRVAQAVGALTVGEGPEADLQVHSVQVERLHTTFGLR
                     RGDDELGPVQVGTPGRHHARNAAVAIAVAIELGVPALDAVAAIGAYRGVARRFEVLGE
                     VRGATVVDDYAHNPGKVRALLASADEAGWSRVVAVFQPHRYTRTRDLLGDFGAALALA
                     DVVAVTEIYGAGEDPLPGVSGRSLIEEVLDVRPWAEVAWVRSLDDAAAWLRSTLRPGD
                     LCLTVGAGDISSLGRRLVAEAGDRAS"
     gene            3519..>4221
                     /locus_tag="VNQ33_08655"
     CDS             3519..>4221
                     /locus_tag="VNQ33_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ98215.1"
                     /translation="MTVPPAAVDALADRLPPLVRRDVPTGPLTTYGVGGPAALAVTVR
                     DEDELAAVARAVAAGEGPVPVLVVGLGSNLLVSDAGFDGLVVVLGPAFADIAIAGTTV
                     RAGARAKLPVVARRTAGAGLTGFEWAAGVPGSVGGAVRMNAGVPDAEIGDCLTRVRVV
                     DLASGEDGVVPASDLSLGYRSSSIRPEQVVVWAELELAAADADVARAAIKEKTQWRRD
                     NQPGGRNAGSVFTNPE"
BASE COUNT          482 a         1666 c         1448 g          625 t
ORIGIN      
        1 gcgctctgga cggtgctggg caccgtcgcc ctgttcgcca cgctgatggt ctcggtcgac
       61 ttctggcgcc gcttcaccaa gccgttcatg gccatcgcca tcgccatgct cgtgctggtc
      121 ctcgtccccg gcatcggcgt gaccgtgaac ggcgccaccc gctggctggg cgccggcccg
      181 ttccagatcc agccgtccga gttcgccaag ttcgccctgc tgctgttcgt ggccgacctg
      241 ctcgaccgcc gctccgacaa gaccgccgac tggcgctgga cgctggcgcc ggtcgtggcc
      301 tacctgggcc tgatcgcggc gctgatcatg ctccagccca acctgggcac cacgatcatc
      361 atcgccttca tggtgctgac gatgctcctg gtggccggtg tgccgctgct gccgctctcg
      421 gccgtcgcgg ccgccggagc gatggtcgcc accgcgttcg tcgcctacac cccgttccgc
      481 cgcacccgct tcctgcgctt cctcaacccg tgggccgacc cgcagaacac cggcctccag
      541 aacgtgcagt cgctcgtcgg catcgccaac ggcgggctcc tcgggcgggg cctcggccgc
      601 tccaccgcca agtggggctt cctgccgttc gcccacacgg acttcatctt cgccgtcatc
      661 ggcgaggagt tcggcctggt gggcgccatg tcggtcgtgc tgctgttctt cggcatcacg
      721 tgcaccggcg cgtggatcgc cgcccgggcc aaggaccggt tctcgatgct cgtcgccacc
      781 gggatcacgg cgtggatcgg gatccaggcg ttcatgaaca tcggtgcggt catcggcatc
      841 atgccgatca cgggcgtgcc gctcccgttc atctccttcg gcggctcctc cctcatgatc
      901 aacctggccg ccgccggcat gctcctcaac atcgcccgcc atcccgccga gcacctgccc
      961 cggtcgtccc gccgagccaa gcgctcatga gcgagccgac ctgggccctc gtcgccgggg
     1021 gcggcactgc cggccacctg ctgcccggcc tgtcggtggc tcgggccctc gtggccgagg
     1081 ggcgcgatcc ggccacgatc cacttcgtgg gcgccgagcg cggcccggag caggagctgg
     1141 tgccggccgc cggcttcacc gtcgacgtgc tccccggccg cggcatccag cgcaagctct
     1201 ccgccgccaa cgtcgctgcg gtcctcgatc tgttcaaggc cctgttccgg ggcatcggca
     1261 tcgtccgcac gcgcaagccg tcggtcgtca tcgtgctcgg cggctacgcc agcttcgcct
     1321 gcggggtggg cgccgtcttc ggccgggtgc cgctcgtgct gctcgagcag aacaagaagg
     1381 cgggcgccgt caaccgggcg ctgcgccgct tcgccgccgc ctcggccgtg tcgttcgagg
     1441 gcaccgacct cccgaaggcc accgtcaccg gcaacccgct gcgccccgag atccgccaga
     1501 tggccgagca ccccgatccg gccgcggccc gggcggccct cgacctgccg gccgaccgca
     1561 cggtcatcgg cgtgttcgcc ggatccctcg gctcccgccg catcaacgat gcggtgcagg
     1621 gcgtcgtcga acgctgggcc caccggtccg acctcgccgt gcgccacgtg gtcggccgca
     1681 acgggttctc cgacggcagc gacggcccca ccggcctccc ggccgacggc atcgtctacc
     1741 agcgggtgcc ctacgaggac cggatgcacc tcctgctcga cgccagcgac ctggccgtaa
     1801 cccgctccgg cggatcggtc ttcgagctca tggcggcggg ccttccggcc gtcctggtgc
     1861 cgctgcccat cgccacccgc gaccaccaga tggccaacgc gcgggcggtc gaggccctcg
     1921 gcggtgcggt cgtggtcccg gactcggagc tcgacaccga tcgcctcgag gccgagctga
     1981 ccgcgctgct gggcgaccgc gagcgcctcg cggccatggc cgccgccctg cgcgccggtg
     2041 ccgccatcga cgccgccgag caggccgctg ccgttgtcga acgggtcgct cgtggctgag
     2101 cccgtcaccg gcctctcggc ccctcggacc gtccacctcg tcggcatcgg cggggccggc
     2161 atcagcgcca tcggcatcat cttggccacg atgggccacc gggtcaccgg gtccgacgtc
     2221 aacgagacgt cggcgtggcc gtcgctgatc gacgcgggcg tccaccccga ggtcgtcgct
     2281 ccaggcgggc tgttcgcctc cgcccacgcc cacggcgccg agatcgtggc ccactccacg
     2341 gcgttcccgc ctaccgagcg agacctcgaa gcggccgccg cggccggctg cacggtgctc
     2401 gaccgggccg ggatcctcgc gctcatctgc gcggcccggc ccaccgtcgc cgtgtcgggc
     2461 acgcacggca agaccagcac gaccgcgatg ctcgccaccc tgctcgacgg cgccggcacc
     2521 gatccctcgt acctcgtggg cgccaagccc gtcggcctgg ggcgggcggc gcactgggca
     2581 ggcgggggga gcggtgcgtt cgtggtggag gccgacgaga gcgacggcac cttcctgcgc
     2641 ctcggcggcg acatcgccgt ggtcaccaac atcgacgagg accacctcga ctactggcgc
     2701 gacatcgacg ccatcgaggc cgcgttcttg cggttcctca ccgaagcccg cgtcggcgtc
     2761 gtcggcatcg acgacccccg cggcgagcac atcaccgatc cccgtgcgct ccgggtggcc
     2821 caggccgtgg gtgcgctgac ggtaggcgag gggcccgagg cggacctcca ggtccactcc
     2881 gtccaggtcg agcgcctcca caccacgttc ggtctgcgac ggggcgacga cgagctcgga
     2941 ccggtgcagg tcggcacgcc ggggcgccac cacgcccgca acgcagcggt cgccatcgcc
     3001 gtggccatcg agctgggcgt gcccgctctc gacgcggtgg ccgccatcgg cgcctaccga
     3061 ggcgtcgccc gccgcttcga ggtcctgggc gaggtgcggg gcgccacggt cgtcgacgac
     3121 tacgcccaca acccgggcaa ggtccgcgcc ctgctcgcct cggccgacga agccggctgg
     3181 tcccgcgtcg tcgccgtgtt ccagccgcac cgctacacgc ggacgcggga cctcctcggc
     3241 gacttcggtg ccgccctggc gctggccgac gtcgtcgccg tcaccgagat ctacggcgcc
     3301 ggtgaggacc cgctgcccgg cgtgtcgggc cgctcgctca tcgaggaggt gctcgacgta
     3361 cggccctggg ccgaggtcgc ctgggtgcgc tcgctcgacg atgccgccgc ctggctgcgc
     3421 agcacgctgc ggcccggcga cctgtgcctc accgtcggcg ccggcgacat ctcgagtctc
     3481 ggccggcgtc tcgtcgccga agccggggat cgggcctcgt gaccgtgcca cctgccgccg
     3541 tcgacgccct cgccgaccgc ctcccgccgc tcgtccggcg cgacgtcccc accggtccgc
     3601 tcaccaccta cggcgtcggc ggccccgccg cgctcgccgt cacggtgcgc gacgaggacg
     3661 agctggctgc cgtcgcccga gccgtcgccg cgggggaggg cccggtcccg gtgctcgtcg
     3721 tcggcctcgg gtcgaacctc ctcgtgtccg acgccggttt cgacggtctc gtcgtcgtcc
     3781 tcggtccggc gttcgccgac atcgccatcg ccggtaccac ggtccgcgcc ggtgcccggg
     3841 ccaagctgcc ggtggtcgcc cgccggaccg cgggtgccgg cctcaccggg ttcgagtggg
     3901 cggccggggt gcccggctcg gtcggcggcg ccgtccggat gaacgccggc gtgcccgacg
     3961 ccgagatcgg cgactgcctc acgagggtcc gcgtcgtgga cctggcctcg ggcgaggatg
     4021 gagtcgtgcc ggcgtccgat ctgtccctgg ggtaccggtc ctcgtcgatc cggcccgagc
     4081 aggtcgtcgt ctgggccgag ctggagctgg ccgccgccga tgccgacgtg gcccgagcgg
     4141 ccatcaagga gaagacccag tggcggcgtg acaaccagcc cggtggccgc aacgccggct
     4201 cggtgttcac gaaccccgag g
//
LOCUS       DATMFS010000459         2707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_86054, whole genome shotgun sequence.
ACCESSION   DATMFS010000459 DATMFS010000000
VERSION     DATMFS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86054"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VNQ33_08660"
     CDS             <1..632
                     /locus_tag="VNQ33_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWJ98216.1"
                     /translation="TASGLAVAGLAGCSLSQASTHDPSVDTGEKKPWGGEVDEQGLAK
                     PDITLTDMNGEPFPFVEKTKGKLALLFFGYTNCPDVCPVTLSSLARALDKIGTGPGSD
                     PMVLFVGVDTARDTPEQLKTYLGRINPAFLGLTGTDHQIADANKQVFNPPITLEPPDA
                     NGEYAVGHMSKVFAYSPTDDLAHRYYNGDKVRPQTWVQDLPRLAKGQFQ"
     gene            646..1548
                     /locus_tag="VNQ33_08665"
     CDS             646..1548
                     /locus_tag="VNQ33_08665"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWJ98217.1"
                     /translation="MSWWCSATARNWTWTPKPYLGAWVMLIGVLAVAAWWHGRGRHQE
                     PKLDAREAAELSEGSGPYPGSGTSRVVSFVFGAIGLWACLDWPLATLGAGYLATAQMV
                     RQVLMVFAVAPLLLYACPAGLAVHLVGWGRRLTVLRWAARPLIAVPVAAVTLLAVNAP
                     AVLDPLVATPYGAFAMDLAWIVAGFVLWMPVQCPHPGVRRLDGAFALGYLILQSIVPL
                     LPGFFMTWADEAIYRTYDLAPRAIAGFDAVNDQQTAAAILQVGGMVFLWVQISFRFLH
                     WGYQELEKDRQPRKPITESRATTS"
     gene            1559..2248
                     /locus_tag="VNQ33_08670"
     CDS             1559..2248
                     /locus_tag="VNQ33_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWJ98218.1"
                     /translation="MFVKICGITREDDALLAVAMGADALGFNFVSGSRRQIAPAVARD
                     IARRLPPEILTVGIFRDEASSRVVEIVNSAGLRAAQLHGRESAETTRWVRERVPYVIK
                     AFSAGDPALEQAAGFGADAVMIDSPGGGTGEVFDWTLAEGRPDNVKVVLAGGLDPGNV
                     ADAIRRVRPWGVDVATGVEAERGEPGQKDARKVRLFIENARRAAPDVEDEFGPDPGTG
                     APFDWMQDGSP"
     gene            2360..>2707
                     /locus_tag="VNQ33_08675"
     CDS             2360..>2707
                     /locus_tag="VNQ33_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWJ98219.1"
                     /translation="MTSTTPDSSFMGEPDATGRFGRFGGRFIPETLMPACIELDTAFR
                     DAWADPAFRAELDELLRSYAGRPSPLTECHRLSEELGVRVLLKREDLNHTGSHKINNV
                     LGQALLTKRMGKRR"
BASE COUNT          376 a         1019 c          913 g          399 t
ORIGIN      
        1 tcaccgcctc gggcctcgcc gtcgccggcc tggccggctg cagcctcagc caggccagca
       61 cgcacgaccc ctcggtggac accggggaga agaagccctg gggcggcgag gtcgacgagc
      121 agggcctcgc caagccggac atcacgctca ccgacatgaa cggcgagccg ttcccgttcg
      181 tcgagaagac gaagggcaag ctcgcgttgc tgttcttcgg gtacacgaac tgccccgacg
      241 tctgccccgt cacgctcagc tccctcgcac gcgccctcga caagatcggg accggcccgg
      301 ggtccgatcc gatggtcctg ttcgttggcg tcgacaccgc ccgggacacg cccgagcagc
      361 tgaagaccta cctcggccgc atcaacccgg ccttcctcgg gctcaccggc acggaccacc
      421 agatcgccga cgccaacaag caggtcttca acccgcccat cacgctcgaa ccgcctgacg
      481 ccaacgggga gtacgccgtg ggccatatgt cgaaggtgtt cgcctacagc ccgaccgacg
      541 acctggcgca ccggtactac aacggcgaca aggtccgccc ccagacctgg gtgcaggacc
      601 tgccccgtct ggcgaagggc cagttccagt gagagacggc cgatcgtgag ctggtggtgc
      661 tcggcgaccg cccggaactg gacgtggacg cccaagccgt acctcggcgc ctgggtgatg
      721 ctcatcggcg tcctcgccgt cgccgcgtgg tggcacgggc ggggccgcca ccaggagccg
      781 aagctcgacg cccgtgaggc tgccgagctg agcgagggga gcggcccgta ccccgggtcg
      841 ggcacctcgc gggtggtgtc gttcgtcttc ggcgccatcg gcctctgggc ctgcctcgac
      901 tggccgctcg ccacgctggg cgccggctac ctggccaccg cccagatggt gcgccaggtg
      961 ctgatggtgt tcgccgtcgc cccgctgctc ctctacgcct gcccggctgg cctcgccgtg
     1021 cacctggtgg gctggggtcg tcggctcacg gtcctgcggt gggcggcccg gccgctgatc
     1081 gccgtgccgg tcgctgccgt cacgctgctc gccgtgaacg cgcccgcggt cctcgacccg
     1141 ctggtcgcca cgccctacgg cgccttcgcc atggacctgg cgtggatcgt ggccggcttc
     1201 gtgctgtgga tgccggtcca gtgcccgcac cccggcgtcc gccgcctcga cggcgccttc
     1261 gccctcggct acctgatcct ccagtcgatc gtgccgctgc tcccgggctt cttcatgacc
     1321 tgggccgacg aggccatcta ccgcacctac gacctggcgc cgcgtgccat cgccggcttc
     1381 gacgccgtca acgaccagca gaccgccgcg gccatcttgc aggtgggcgg catggtcttc
     1441 ttgtgggtcc agatctcgtt ccggttcctc cactggggct accaggagct ggagaaggac
     1501 cggcagcctc ggaaacccat cacggagagc cgggcgacca cctcgtagat tctccgtggt
     1561 gttcgtgaag atctgcggga tcacccggga ggacgacgcc ctcctggcgg tggccatggg
     1621 ggccgacgcg ctcgggttca acttcgtctc gggctcacga cgccagatcg cgcccgccgt
     1681 cgcccgcgac atcgcccggc gcctgcctcc ggagatcctc accgtcggga tcttccgcga
     1741 cgaagcgtcg tcgcgagtgg tggagatcgt gaacagcgcc gggctccgag ccgcccagct
     1801 gcacggccgc gagagcgccg agaccacccg ctgggtccgc gagcgggtgc cctacgtgat
     1861 caaggcgttc tcggccggcg atccggcgct cgagcaggcg gccggcttcg gcgccgacgc
     1921 cgtgatgatc gactcgcccg gcggcggtac gggcgaggtg ttcgactgga ccctggccga
     1981 ggggcggccc gacaacgtga aggtggtcct cgccggcggg ctcgaccccg gcaacgtggc
     2041 cgatgccatc cgccgggtgc ggccctgggg cgtcgacgtc gccaccggtg tcgaggcgga
     2101 gcggggcgaa ccaggccaaa aggacgcccg caaggtccgg ctgttcatcg agaacgcccg
     2161 cagggcggcg cccgacgtcg aggacgagtt cggtcccgat ccgggcacgg gtgccccgtt
     2221 cgactggatg caggacggtt cgccgtgacg gcggcacgcg tcgacgggcg atggcgccgc
     2281 tgctgaacac cggatcgggc gcctccggcg cgcccgccgt tgtccgtccg ccatccctgc
     2341 tccgtaacgt gaggacccca tgacttccac cacgcccgac tcgtccttca tgggcgagcc
     2401 cgacgccacc ggccgcttcg gccgcttcgg cggccggttc atccccgaga cgctcatgcc
     2461 cgcgtgcatc gagctcgaca ccgcgttccg cgacgcctgg gccgaccccg ccttccgggc
     2521 cgagctcgac gagctgctcc gcagctacgc cggtcggccc tcgcccctga ccgagtgcca
     2581 ccggctctcc gaggagctcg gcgtgcgcgt cctcctcaag cgcgaggacc tgaaccacac
     2641 cggctcgcac aagatcaaca acgtgctcgg ccaggccctg ctcaccaagc gcatgggcaa
     2701 gaggcgc
//
LOCUS       DATMFS010000460         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_39674, whole genome shotgun sequence.
ACCESSION   DATMFS010000460 DATMFS010000000
VERSION     DATMFS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39674"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VNQ33_08680"
     CDS             complement(<1..1119)
                     /locus_tag="VNQ33_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWJ98220.1"
                     /translation="MTDDRRRLLTIAVSGSLAVWAALILLGLPNERSAVILSNLGQVV
                     GPALAAIGCLLASRRAISDRQSAGWKLLGASAASWCLGQIAWTYYEWSGAAAPFPSLA
                     DVGYVLAVPLALSAIWTLTVRSSTSSWAVAVLDGLILAGGLLAISWPLVLGEAWEQGG
                     DSPFLFALSLAYPVGDLVVVSAVLLAIMRTDRDRDAVPLVPIAIGLIVLGVADSIFVW
                     TTMQGTEQAVSIADVGWAAGYLILLLAAMQYPLPVASGRTEVHAAPNLRRAALPLTIV
                     TIAWAIRMALVATGQPEDEFLTMLTVATVALVLVRFLMTMRENQELTGSLEEKIGELT
                     AREQQLSHQALHDPLTGLANRRLFSDRVEHALNRSRRTG"
     gene            complement(1228..2139)
                     /locus_tag="VNQ33_08685"
     CDS             complement(1228..2139)
                     /locus_tag="VNQ33_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HWJ98221.1"
                     /translation="MDAVRTPDDRFSGLPGWGFEPRYVEIDDGEGGSLRVHYVDEGPG
                     DAPPVLLLHGEPSWGYLYRHMIPVLSAAGHRCIVPDLIGFGRSDKPTRQDDHTYARHV
                     EWMRQALFDRLDLTGITFFGQDWGGLIGLRLVAEHPERYARVAVGNTGLSTGDRPLGE
                     AFLAWQRFARTTEVFPVGAIVDGGCTSALAPEVIAGYDAPFPDDAFLAGPRVLPSLVP
                     TTPDDPASAANRAAWEALGRFDQPFLCTFSDADPITKGGERPFLKLVPGTAGQAHTTI
                     EGGGHFLQEDRGPELAAVLIGFIASTA"
     gene            complement(2162..>2374)
                     /locus_tag="VNQ33_08690"
     CDS             complement(2162..>2374)
                     /locus_tag="VNQ33_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ98222.1"
                     /translation="VSALLARPEAVVSRGRLLDAGWPDGQPAGSANGQILDGRIKLLR
                     RRLVGVGLEIHTVRGVGFVLTAKPVA"
BASE COUNT          375 a          789 c          912 g          298 t
ORIGIN      
        1 gccggtgcgg cggctgcggt tgagggcgtg ctccacccgg tcgctgaaca gccggcggtt
       61 ggcgagcccg gtgagcgggt cgtgcagcgc ctggtggctg agctgctgct cccgggcggt
      121 gagctcgccg atcttctcct cgagcgatcc cgtcagctcc tggttctccc gcatcgtcat
      181 caggaaccgg accaagacca gggcgacggt ggccaccgtc aacatggtga ggaactcgtc
      241 ttcgggctga ccggtcgcca cgagggccat gcggatcgcc caggcgatcg tcacgatcgt
      301 gagcggcagg gcggcgcggc ggaggttggg agcggcgtgg acctccgtcc gccccgaggc
      361 gacgggcagg gggtactgca tcgcggcgag gagcaggatc aggtagccgg cagcccagcc
      421 gacatcggcg atcgagacgg cctgctcggt cccctgcatg gtcgtccaca cgaagatgct
      481 gtcggcgacg ccgagcacga tgaggccgat ggcgatgggc acgaggggca cggcgtcgcg
      541 atcgcggtcg gtgcgcatga tcgccagcag cacggccgag acgaccacga ggtcgccgac
      601 cgggtaggcg aggctgaggg cgaacaggaa cgggctgtcg ccgccctgct cccaagcctc
      661 gccgaggacg agcggccagc tgatggcgag gagcccgccc gccaagatga gcccgtcgag
      721 caccgccacg gcccacgacg acgtgctgga gcggacggtc aaggtccaga tggcggagag
      781 ggcgagcggg acggccaaga cgtagcccac atcggccagc gacgggaacg gggcggcggc
      841 gcccgaccac tcgtagtagg tccaggcgat ctggccgagg caccacgagg ccgccgacgc
      901 gccgagcagc ttccacccgg ccgactggcg gtcgctgatc gcccggcgcg acgccagcag
      961 gcagccgatc gcggcgagcg ccggccccac gacctggccc aggttggaca agatcacggc
     1021 gctgcgctcg ttcgggaggc ccagcaggat cagcgccgcc cacaccgcga gcgagccgct
     1081 gacggcgatg gtgaggaggc ggcggcggtc gtcggtcatg gagggggcgg tgaaccgcca
     1141 gcattcgcgg aagcccacca cacctggtga tcggccgatc acggcaggaa tccagggccg
     1201 ctgcgccaga tcaggtcatt tggcccatca agccgtactg gcgatgaagc cgatgaggac
     1261 cgctgcgagc tccggccccc ggtcctcctg caggaagtgg cccccgcctt cgatggtggt
     1321 gtgggcctgg cctgcggttc cgggcaccag cttgaggaac ggacgctcgc cgcccttggt
     1381 gatgggatcg gcatcgctga acgtgcacaa gaagggctgg tcgaagcggc cgagcgcctc
     1441 ccaggcagcc cggttcgctg ctgaggcagg gtcgtcgggc gtcgtgggca cgagcgacgg
     1501 gagcacccgg gggccggcca agaacgcgtc gtcggggaag ggagcgtcgt agccggcgat
     1561 cacctccggg gcgagcgcgg aggtgcaccc accgtcgacg atggcaccga ccgggaagac
     1621 ctcggtcgtc cgggcgaagc gctgccaggc gaggaacgcc tcgcccaggg gccggtcgcc
     1681 ggtggacagc ccggtgttgc cgacggcgac gcgggcgtag cgctccgggt gctcggcgac
     1741 gaggcgcagg ccgatgagcc cgccccagtc ctgcccgaag aacgtgatgc cggtcaggtc
     1801 gaggcggtcg aacagggcct ggcgcatcca ctcgacgtgg cgggcgtagg tgtggtcgtc
     1861 ttggcgggtc ggcttgtccg atcgcccgaa cccgatcagg tccgggacga tgcagcggtg
     1921 gccggcggcc gacagcaccg ggatcatgtg ccggtacagg tacccccacg acggttcgcc
     1981 gtggagcagc agcaccggcg gggcgtcgcc cggtccctcg tcgacgtagt ggacgcggag
     2041 cgatccgcct tccccgtcgt cgatctccac gtaccggggt tcgaaccccc agcccgggag
     2101 tcccgagaag cggtcgtctg gtgtgcgcac ggcgtccatg gacgccgacg gtaccggtgc
     2161 ttcaagccac cggcttggcc gtcaagacga agccgacgcc gcggaccgtg tggatctcga
     2221 ggccgacgcc gaccaggcgg cggcgcagga gcttgatgcg gccgtcgagg atctggccgt
     2281 tggcgctgcc ggcgggctga ccatcgggcc acccggcatc gagcagtcgg ccgcgggaca
     2341 cgacggcttc gggtcgggcc aggagggcgg acac
//
LOCUS       DATMFS010000461         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_132240, whole genome shotgun sequence.
ACCESSION   DATMFS010000461 DATMFS010000000
VERSION     DATMFS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132240"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VNQ33_08695"
     CDS             <1..437
                     /locus_tag="VNQ33_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98223.1"
                     /translation="PGTTSQTLHLDGVSNLSKKATAFEAPTTTPAFHTWAVEILPEGS
                     SVRFNFYVDNVKVHTFADTKHAWTSADASGTWDMALNLAVGGRWTGNPDGTLGYLDLL
                     NRCSISGTAPSGCTTTGIKRVNWANPATSTYEIDYVRVYTPA"
     gene            651..1745
                     /locus_tag="VNQ33_08700"
     CDS             651..1745
                     /locus_tag="VNQ33_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWJ98224.1"
                     /translation="MSAYIGGTGPSSTRGEGGGMEQIGRRSMLTGAAVAGVATAGAVL
                     GSALPAAAAAVGPWEYVAPGSSIQTAITGGAKAIQLGAGTYNVTSAIVLQRAVSIRGV
                     GQSTRIVCTASPTISEVITISGAIDGVYLGDFVIDVAGKANVGINLDINGTSGNYQGE
                     PDAVCRLDNLWIYSPVQDGIVYQGTDTQATVTTRVRVRQAGRYGYSILAPDNMFIGCE
                     ATTASGGQSGFYVGSANCQYHSCKAWYCRGYGWHVKGTRNAFIGCESQDTKLHGWYIE
                     YDKNAFVGCVADTAGMYDVGGTSGTADGFYVDPGVRTTMSGCLAFDRKPGGHAAQQRY
                     GFNILTALQSGGLFVANTGWDNATGLVNLR"
     gene            complement(1752..>2240)
                     /locus_tag="VNQ33_08705"
     CDS             complement(1752..>2240)
                     /locus_tag="VNQ33_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98225.1"
                     /translation="LLAGLVLLVVAVGFPLGGPLHDLADRVFTPITVDRALRRSTTRA
                     LERLRPSIGALPDVPGAEPAAVEPRYNCTAKGDDPVAQPELRRRWDVPASEAGGAASS
                     VLAALGADGWTTSGTGVAHRSIDDDTFEAAVSTTADGRQASVALTVRVADVEPCDVGF
                     GR"
BASE COUNT          365 a          810 c          762 g          303 t
ORIGIN      
        1 cacccggcac gacctcccag acgctccacc tcgacggcgt ctccaacctc tccaagaagg
       61 ccaccgcctt cgaggccccc accaccaccc ccgccttcca cacctgggcc gtcgagatcc
      121 tccccgaagg cagcagcgtc cggttcaact tctacgtcga caacgtgaag gtccacacct
      181 tcgccgacac caagcacgcc tggacctcgg ccgacgcctc gggcacctgg gacatggccc
      241 tcaacctcgc cgtcggcggg cgctggaccg gcaaccccga cggcaccctc ggctacctcg
      301 acctgctcaa ccgctgctcg atcagcggca ccgctcccag cggctgcacc accaccggca
      361 tcaagcgcgt caactgggcc aacccggcca cgagcaccta cgagatcgac tacgtccggg
      421 tctacacccc ggcctgatcg ccgcccggcg ggcctcccgt cgtctcggag gcgaggcgct
      481 ttccgagcac tcgcctccga gatcgctcac cgcccggcag ccctggcgga tcggctggcg
      541 ggccggcctc cgtgcccatg ggaccgagcg acccggcccg gacgccaggc agggccgaac
      601 ggtccgggtg acacaagatc tgcttgacgc cgccccggca cgggagaagg atgtcggcgt
      661 acatcggcgg gacggggcca tcgagcaccc gtggcgaagg aggcgggatg gagcagatcg
      721 gtcgtcgatc catgttgacg ggggccgcgg tcgctggcgt tgcgaccgcg ggagcggtcc
      781 tcggatccgc cctgcctgcg gcggctgccg cggtcggccc atgggagtac gtcgcgcccg
      841 ggtcgtcgat ccagaccgcg atcacgggcg gtgccaaggc gatccaactc ggggctggca
      901 cctacaacgt gacctcggcg atcgtcctcc agcgggcggt ttcgatccga ggcgtcgggc
      961 agagcacgcg catcgtctgc accgcgtctc ccacgatctc tgaagtgatc acgatcagcg
     1021 gagcgatcga cggcgtctac ctcggtgact tcgtgatcga cgtggcgggc aaggccaacg
     1081 tggggatcaa cctcgacatc aacgggacgt cggggaacta ccagggcgag cctgatgcag
     1141 tctgccgact cgacaacctg tggatctact cgcccgtcca ggacgggatc gtgtaccagg
     1201 gcaccgacac ccaggccacg gtcaccaccc gagtccgagt ccggcaggcg ggccgctacg
     1261 gctacagcat cctcgccccg gacaacatgt tcatcggttg cgaggccacg acggcgtccg
     1321 gagggcagtc aggcttctac gtcggctcgg ccaactgcca gtaccactcc tgcaaggcgt
     1381 ggtactgccg gggatacggc tggcacgtga agggcactcg caacgcgttc atcggctgcg
     1441 agtcgcagga caccaagttg cacggctggt acatcgagta cgacaagaac gcgttcgtcg
     1501 gctgcgtcgc cgacacggcc ggtatgtacg acgtgggggg cacctcgggc accgcagacg
     1561 gcttctacgt ggacccgggc gttcggacca cgatgagcgg gtgcctcgcc ttcgaccgca
     1621 agccaggggg ccacgcggca cagcagcgct acggcttcaa catcttgacc gcgctccaga
     1681 gcggcggcct cttcgtcgcc aacacgggtt gggacaacgc caccgggctc gtcaacctgc
     1741 gctgaacgcg gtcatcgccc gaagccgaca tcgcacggct cgacgtcggc cacccgcacg
     1801 gtgagcgcca cggacgcctg ccggccgtcg gccgtcgtgg agacggcggc ctcgaacgtg
     1861 tcgtcgtcga tgctgcggtg ggcgactccg gtcccgctcg tcgtccagcc gtcggcaccg
     1921 agggcggcca gcaccgatga cgcggcgccg ccggcctcgg aggccgggac gtcccaccga
     1981 cggcgcagct cgggctgggc caccggatcg tcgcctttgg ccgtgcagtt gtagcgcggc
     2041 tcgaccgcgg cgggttcggc gcccggcacg tcgggcaggg ctccgatcga ggggcgcagg
     2101 cgttcgaggg cgcgggtggt ggagcggcgc agggcccggt cgacggtgat gggcgtgaag
     2161 acccgatcgg ccaggtcgtg gagcggtccg ccgagcggga agccgacggc gacgaccagc
     2221 aggacgagcc cggccagcag
//
LOCUS       DATMFS010000462         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_132273, whole genome shotgun sequence.
ACCESSION   DATMFS010000462 DATMFS010000000
VERSION     DATMFS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132273"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VNQ33_08710"
     CDS             <1..1343
                     /locus_tag="VNQ33_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98226.1"
                     /translation="GTTMIRRRNAILLALGLLAPLALVDSPAGAIDQDLVASVTAGPP
                     GTTITVSSASCTDPVDDNAYLMASLYVGTAPNQQIAAFGSGSTGSATLTVPDWVDPDQ
                     PAVIEASCELYSDNGNDGYLEYDPIAFDIEPGSGTPVQVRTFSRTELLAGQGLTVTGS
                     CGPALADSLVFAVVGSGTDQTGESFETIAGQGIAQAGADGSFELPLFITNAFVGIGIE
                     ENGSELTALTDEEPMDVPAGDYTAFIYCGIDTADDSIQFLEPQLLVITGSAPTSDIDL
                     LNTAGSTAVSLTGICEAGDVAGSIEAMSTEELANGFGEPIFDPSARGNRFQAARVASA
                     RAASVRTATRGPANLVRSPDGATRVIADGEVRDFDARPAADGTWQVGDDAGFREGVVI
                     ATALCGDPMADGFIYDPQGVTIEVVEPTTTVPVTVPVTVPAPANAIPGTPTYAG"
     gene            1377..2003
                     /locus_tag="VNQ33_08715"
     CDS             1377..2003
                     /locus_tag="VNQ33_08715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWJ98227.1"
                     /translation="MTTLILASGSPRRRLLLGELGVPFEVRPTDVDETPLPGETAEEL
                     VRRLAVAKAEAGLAAASARSGEAVIVLAADTLVSVGGEVLGKPVDEADAARMLRLLSG
                     SRHQVLTGVAVAWADGGAAGRAVEVEVTHVHMASWTDEQIEAYVASGEPMDKAGSYAI
                     QEVGDRFVTEVEGSFDNVVGLPVARTRRLLADAGLDLPEPPGDQPPEV"
     gene            complement(1985..>2908)
                     /gene="dusB"
                     /locus_tag="VNQ33_08720"
     CDS             complement(1985..>2908)
                     /gene="dusB"
                     /locus_tag="VNQ33_08720"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWJ98228.1"
                     /translation="EQPRSIQLYGTNPAVMHDAARQLVTEQGAEHIDLNFGCPVRKVT
                     RHGGGSALPWRRTLFAQVVAAAVKGAGDVPVTVKLRMGIDDDHLTYLDAGPIAETQGA
                     AAVALHARTAQQAYSGKADWSAIARLKAVVHEVPVLGNGDIWVAADALRMVDETGCDG
                     VVVGRGCLGRPWLFRDLAAAFQGEAVDSAPPMAVVAATLRDHARRLAEWHGDHVGVKD
                     IRKHVGWYLQGYPVGPAIRKRLVQADTLDQFEAVLDELDPTTELPPESIGLPRGHQHG
                     PRPVTVPEGFWDDRDSPEPLGAGADAYTSGG"
BASE COUNT          385 a         1076 c         1049 g          398 t
ORIGIN      
        1 caggaaccac gatgatccga cgccgcaacg ccatcctcct cgccctcggc ctgctcgccc
       61 cgctcgcgct ggtcgacagc cccgccggcg ccatcgacca ggacctggtc gcctcggtga
      121 ccgccggtcc ccccgggacg accatcaccg tctcgtcggc gagctgcacc gatccggtgg
      181 acgacaacgc ctacctgatg gcgtcgctct acgtcggcac ggcgccgaac cagcagatcg
      241 ccgccttcgg cagcggttcc accggcagcg ccaccctgac cgtcccggac tgggtcgacc
      301 ccgaccagcc cgcggtcatc gaagcgtcgt gcgagctcta ctcggacaac ggcaacgacg
      361 gctacctcga gtacgacccg atcgcgttcg acatcgagcc ggggtcgggc acgccggtgc
      421 aggtgcgcac cttcagccgg accgagctgc tcgccggtca aggcctcacg gtcaccggct
      481 cctgcggccc cgccctcgcg gacagcctcg tgttcgccgt cgtcggcagc ggcaccgatc
      541 agaccggcga gtcgttcgag accatcgccg gtcagggcat cgcccaggcg ggtgctgacg
      601 gctcgttcga gctgccgctg ttcatcacga acgccttcgt cggcatcggc atcgaggaga
      661 acggctcgga gctgaccgcg ctcaccgacg aggagccgat ggacgtgccc gcgggcgact
      721 acaccgcgtt catctactgc ggcatcgaca ccgctgacga ctccatccag ttcctcgagc
      781 cccagctgct ggtgatcacc ggttcggctc ccacctccga catcgacctg ctcaacacgg
      841 ccggatcgac cgccgtgagc ctgacgggca tctgcgaggc gggcgacgtc gccgggagca
      901 tcgaggccat gtcgaccgag gagctcgcca acgggttcgg tgagccgatc ttcgacccct
      961 cggcacgcgg caaccggttc caggcggccc gggtcgccag cgcgagggcg gcgtctgtcc
     1021 gcaccgccac ccgcgggccc gccaacttgg tccgcagccc cgacggcgcg acgcgcgtga
     1081 tcgccgatgg cgaggtccgc gacttcgacg cgcggcccgc ggccgacggc acctggcagg
     1141 tcggcgacga cgccgggttc cgcgaggggg tcgtgatcgc cacggcgctc tgcggcgacc
     1201 ccatggccga cgggttcatc tacgacccgc agggcgtgac catcgaggtg gtggagccca
     1261 ccaccacggt gccggtcacc gtccccgtca cggtccccgc cccggcgaac gcgatcccgg
     1321 gcacgccgac ctacgccggc tgactggttc cgccggccgt cgcccatgat gggacggtga
     1381 ccacgctgat cctcgcttcg ggctcgcccc gccgccggct gctgctcggc gagctgggcg
     1441 tgcccttcga ggtgcgcccg accgacgtcg acgagacccc gctccccggt gagacggctg
     1501 aggagctggt ccgccggctc gcggtggcca aggccgaagc gggcctcgct gcggcttctg
     1561 ctcggtccgg tgaggcggtg atcgtgctcg cggccgacac gctcgtgtcc gtcggcggcg
     1621 aggtcctcgg caagccggtc gacgaggccg acgccgcccg catgctccgc ctcttgtcgg
     1681 ggagccgcca ccaggtgctc accggcgtag cggtggcctg ggccgacggc ggcgcggccg
     1741 ggcgcgcggt cgaggtcgag gtcacccacg tgcacatggc gtcgtggacc gacgagcaga
     1801 tcgaggcgta cgtcgcgtcg ggcgagccga tggacaaggc cggctcgtac gcgatccagg
     1861 aggtcggtga ccggttcgtg accgaggtcg aagggagctt cgacaacgtg gtggggctcc
     1921 cggtggctcg cacccggcgc ctgctcgccg acgccgggct cgacctgccg gaaccgcccg
     1981 gggatcagcc gcccgaggtg taggcgtcgg cgccggcgcc cagcggctcc gggctgtcgc
     2041 ggtcgtccca gaagccctcg gggacggtga cgggccgggg gccgtgctgg tggccgcggg
     2101 ggaggccgat ggactccggt gggagctcgg tcgtgggatc gagctcgtcg aggaccgcct
     2161 cgaactggtc gagcgtgtcg gcctgcacga ggcgcttccg gatggcgggc ccgaccgggt
     2221 agccctgcag gtaccagccc acgtgcttgc ggatgtcctt cacgccgacg tggtcgccgt
     2281 gccactcggc caggcggcgg gcgtggtcgc gcagcgtcgc cgccacgacg gccatcggcg
     2341 gcgccgagtc gacggcctca ccctggaagg cggcggccag gtccctgaac agccagggcc
     2401 ggccgaggca gccgcggccc acgaccacgc cgtcgcaccc ggtctcgtcg accatgcgga
     2461 gcgcatcggc cgccacccag atgtcaccgt tgccgagcac cggcacctcg tggacgaccg
     2521 ccttgagccg ggcgatggcc gaccagtcgg ccttgcccga gtacgcctgc tgggccgtgc
     2581 gggcgtggag ggcgacggcg gccgcgccct gggtctcggc gatggggccg gcgtcgaggt
     2641 acgtgaggtg gtcgtcgtcg attcccatcc ggagcttgac cgtgaccggc acgtcgcccg
     2701 cccccttcac cgccgccgcc acgacctggg cgaacagggt gcggcgccag gggagggccg
     2761 agccgccgcc gtggcgcgtg accttgcgga ccgggcagcc gaagttgagg tcgatgtgct
     2821 cggccccctg ctcggtgacg agctggcggg cggcatcgtg catcaccgcc gggttggtgc
     2881 cgtagagctg gatcgaccgg ggctgctc
//
LOCUS       DATMFS010000463         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_155684, whole genome shotgun sequence.
ACCESSION   DATMFS010000463 DATMFS010000000
VERSION     DATMFS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155684"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VNQ33_08725"
     CDS             <1..499
                     /locus_tag="VNQ33_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWJ98229.1"
                     /translation="THDMDRKHMQEMFRRARNHRGAAFVEIFQNCNVFNNGAFETITA
                     RSARDDMLIPLVHGEQVRFGAEREKGVAIDHQGRAQIVRVDDVGEDAILVHDEARSEP
                     GLAFTLSRLSAGPHEPTPIGVFRAVERPDYGSETNRQLAAAQDRSGTGDLHALLHSRP
                     TWDVS"
     gene            complement(492..1256)
                     /locus_tag="VNQ33_08730"
     CDS             complement(492..1256)
                     /locus_tag="VNQ33_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWJ98230.1"
                     /translation="MSDVPPRLPTRLDRPIGFAHRGARANAPENTLEAFELALRLGAT
                     ALESDVWLTADGIAVLDHDGVVGGRLRRKPISEIDRADLPAHIPALGDLYDALGTGFD
                     LSLDLKDPRSGPVVIADSLGRDPAMADRLWLCDQDHERLAELRELSPHVRLVDSTRLS
                     RIKEGPERRAARLQELTIDAVNLHHSDWSGGLSTLFHRFGLCTFGWDAQFDRVLDGLL
                     RMGLDGVFSDHVPRLVDALSRNALARGFGIDEQPLS"
     gene            1351..1680
                     /locus_tag="VNQ33_08735"
     CDS             1351..1680
                     /locus_tag="VNQ33_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98231.1"
                     /translation="MATIRASCSDCGDVELTSADVTVRVCSANSAGSYSFSCPTCSRI
                     VVKAAEPRIVELLVTSGVRLVTWHLPEELTEVHEGEPIDHDDLLDFHALLQRDDWIDT
                     LRKMTPG"
     gene            1731..1934
                     /locus_tag="VNQ33_08740"
     CDS             1731..1934
                     /locus_tag="VNQ33_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98232.1"
                     /translation="MDTTWIAPPAVFGIAAAAAASLLRAIAKATGDAEAAHRRFRRLE
                     DGLIPLRVETRRTRESVDRMRRR"
     gene            1944..2339
                     /locus_tag="VNQ33_08745"
     CDS             1944..2339
                     /locus_tag="VNQ33_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWJ98233.1"
                     /translation="MFNMPGGPELLVIFLVALVVIGPQQLPKAMRTFGNVMAEIRKVS
                     SSFQAEMKSAMDSVVDTSATEKPQSGTMAAATPPSPEADAPSVSDDPAEVVARNGDRP
                     AGAAAGPPAAAEPDAPARPAVDPADRAAG"
BASE COUNT          384 a          857 c          878 g          311 t
ORIGIN      
        1 gacccacgac atggaccgca agcacatgca ggagatgttc cgccgggccc gcaaccaccg
       61 tggtgcggcg ttcgtcgaga tcttccagaa ctgcaacgtc ttcaacaacg gtgcgttcga
      121 gaccatcacc gcccgttcgg cccgcgacga catgctgatc ccgctcgtgc acggcgagca
      181 ggtgcgcttc ggcgccgagc gggagaaggg cgtggccatc gaccaccagg gccgggcgca
      241 gatcgtgcgc gtcgacgacg tgggagagga cgcgatcctc gtccacgacg aggcccgctc
      301 ggagccaggg ctcgcgttca cgctgtcgcg gctgtcggcg gggccccacg aaccgacgcc
      361 gatcggggtg ttccgggcgg tggagcggcc cgactacggc agcgagacca accgccagct
      421 cgccgccgcc caggaccgtt cgggcaccgg cgacctgcac gcgctgttgc acagccggcc
      481 cacctgggac gtcagctgag cggctgctcg tcgatcccga agccgcgcgc gagggcgttg
      541 cggctgaggg cgtcgaccag gcgcgggacg tgatcggaga acacgccgtc gaggcccatg
      601 cgcagcaggc cgtcgaggac gcggtcgaac tgcgcatccc agccgaaggt gcacaggccg
      661 aagcggtgga acagggtgct gaggccgccg ctccagtcgc tgtggtgcag gttgacggcg
      721 tcgatggtca gctcctggag ccgcgccgcg cgccgctcgg ggccttcttt gatgcgcgac
      781 aggcgcgtgg agtcgacgag ccggacgtgg ggggacagct cgcgcagctc ggctaggcgc
      841 tcgtggtcct ggtcgcacag ccagagccgg tcggccatcg ccgggtcacg gcccagggag
      901 tcggcgatga cgacgggacc cgagcgcggg tccttcagat ccagcgacag gtcgaacccg
      961 gtgcccagcg cgtcgtagag gtcgcccagg gcggggatgt gggccggcag gtcggcgcgg
     1021 tcgatctccg agatcggctt ccggcggagg cgcccgccga ccaccccgtc gtggtccaag
     1081 acggcgatgc cgtccgcggt gagccacaca tcgctctcga gggccgtggc cccgaggcgg
     1141 agggccagtt cgaacgcctc gagcgtgttc tccggggcgt tcgcccgagc gccccggtgg
     1201 gcgaagccga tcgggcggtc gaggcgggtc ggcaggcggg gcggcacgtc ggacatggtg
     1261 gttcaccaga cgcacgcgag ccagcatcca cccctcaaca tcggtgggac ctggccgatt
     1321 gagtacggac ggagaggtcc aggaggcgag atggcaacga ttcgagcgag ctgcagcgac
     1381 tgcggcgacg tggagctgac gagcgccgac gtgacggtgc gcgtgtgcag cgccaacagc
     1441 gccgggtcgt actcgttctc gtgccccacg tgctcccgca tcgtggtgaa ggcggcagag
     1501 ccccggatcg tggagctgct cgtcacctcg ggcgtccggc tcgtgacctg gcacctcccc
     1561 gaggagctga ccgaggtcca cgagggcgag ccgatcgacc acgacgacct gctcgacttc
     1621 cacgccctcc tgcagcgcga cgactggatc gacacgctgc gcaagatgac gccgggctga
     1681 acccggaagc tcgacgacac aaccagccga gaccccgcta cgcttgcctc gtggacacca
     1741 cgtggatcgc ccctccggcc gtgttcggca tcgctgccgc ggcggccgcg tccctgctcc
     1801 gggcgatcgc caaggccacc ggtgatgcag aggcggccca ccgccgcttc cgtcgcctag
     1861 aagacggcct gatcccgctg cgggtggaga cccgccgcac ccgggagtcg gtcgaccgca
     1921 tgcgccgccg gtaggctcac cgcatgttca acatgccagg gggaccggag ctcctggtca
     1981 tcttcctggt ggctctcgtc gtgatcggac cacagcagct gcccaaggcg atgcgcacgt
     2041 tcggcaacgt catggccgag atccgcaagg tgtccagcag cttccaggcc gagatgaaga
     2101 gcgccatgga ctcggtggtg gacacgtcgg ccacggagaa gccccagagc ggcaccatgg
     2161 ccgcggccac gccaccctcg cccgaggcag atgcgccgtc ggtgagcgac gacccggccg
     2221 aggtggtggc ccgcaacggc gaccgtcccg ccggcgcggc ggcaggcccc ccggcggcag
     2281 cggaacccga cgcgccggcc cgcccggccg tcgaccctgc tgaccgcgcc gcgggctagg
     2341 cgcccggacc gatggccaag accgtcacgg acgcgccgcc ccacgatggg cgcatgtcgc
     2401 tcatggagca cctcatcgag ctccgcagcc
//
LOCUS       DATMFS010000464         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_342066, whole genome shotgun sequence.
ACCESSION   DATMFS010000464 DATMFS010000000
VERSION     DATMFS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342066"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VNQ33_08750"
     CDS             complement(<1..1342)
                     /locus_tag="VNQ33_08750"
                     /inference="COORDINATES: protein motif:HMM:NF004514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98234.1"
                     /translation="MSGTASVAEVRERRVAGFGGLSLAVQERGEAGRPPVVLVHGYPD
                     DHAVWDLVADRLAADHHVVTYDVRGAGASEIPADRAGYRIEALIADLDAVIGATSPDR
                     PVHLVAHDWGSIQSWSAVTDPVVGRRFASFTSMSGPGLDHVAAWIRAHRIPGRGRWRD
                     ARRQAVRSWYIAAFRTSIPAKVWERGRLADGFPLLLERVDGARVDDRWPGPNLVANGV
                     NGIELYRANMYGRTAPKVPPTTQVPVQLVVPVGDRYVTPALLDGMERVAPNLVRREVG
                     GGHWIPRSNPDLVARLVAEHVAVVEAGRALTPDPERDVVVVTGAGSGIGRSVSLAFAE
                     RGAHVIATDLRTDTVARTAELCRLLGGTAESHALDVTDGDAFDELAARVAAEHGAPSI
                     VVNNAGLGMAGAFLDTTDEDWDLLLDVNLRGVIRGSRTFGRLMVDGGRAGHIVNI"
     gene            complement(1339..2217)
                     /locus_tag="VNQ33_08755"
     CDS             complement(1339..2217)
                     /locus_tag="VNQ33_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWJ98235.1"
                     /translation="MKTATPPPTPARRGHAPTPDQLDAFRAVQQLAYRAAADVAATLE
                     PGMTERQAALALRRRLEADGVDDWFHLPFAWFGDRAAFWHFRVPLQFLPTNRRLEEGM
                     GFILDAAPVVRGATADIGFTATCGANPAVEQIMDDLAAHRTLIVDQVRAGRTFREVYE
                     AVDRLAAEQGYANRHHAYPAHVIGHAIEPLDPGPGIGLTPARFGRRHVRSLVRTIAVG
                     RRERWSPLWSGEPRSGHPPVAGLWAMEPHLGLRGAGAKFEEILVITDDDAFWLDDDLP
                     HVRRWADRGITPTGAR"
     gene            2364..2837
                     /locus_tag="VNQ33_08760"
     CDS             2364..2837
                     /locus_tag="VNQ33_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98236.1"
                     /translation="MSPDHNVPTEPLSPFQGGAAAHPARQAWLLEELEQWFAGDLPDG
                     RDRRRIVAQALRSATEDAHHWLDQRLPCDHLLRVDLHLCNKAGPRHSVFPSLPTPLGL
                     LLICLDQCQVGDEVVLVQELAKSGRVEARATWPFGMDEAETLTRPVRVPVGANQA"
     gene            3176..3322
                     /locus_tag="VNQ33_08765"
     CDS             3176..3322
                     /locus_tag="VNQ33_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98237.1"
                     /translation="MAVDRTKLAERLAAITPFRNRAPMAPSTTRADVDAHTTAFAGAV
                     TAIW"
     gene            complement(3332..>3751)
                     /locus_tag="VNQ33_08770"
     CDS             complement(3332..>3751)
                     /locus_tag="VNQ33_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98238.1"
                     /translation="GWERPAHAEHAPSGFQAVFADERRSVDSPEFARAVQRFRTAAVT
                     IPMVFVAVVFPYLFVIPLLAHRGYSMLALLALPVVGGGLMTLWSARCRKELGLVAEQI
                     EAGASTWDKEIAFTQSYWASMRKPSEPWTGPLPDAAR"
BASE COUNT          554 a         1382 c         1366 g          449 t
ORIGIN      
        1 cgatgttcac gatgtggccg gcccggcccc cgtcgaccat gagccggccg aaggtgcgcg
       61 agccccggat gacgccgcgc aggttcacgt cgagcagcag gtcccagtcc tcgtcggtgg
      121 tgtcgaggaa ggcaccggcc atgccgagcc cggcgttgtt gaccacgatc gacggggcac
      181 cgtgctcggc ggcgactcga gcggccagct cgtcgaacgc gtcgccgtcg gtcacgtcga
      241 gggcgtggga ctcggcggtg ccgccgagca ggcggcacag ctcggcggta cgggcgacgg
      301 tgtcggtccg caggtcggtg gcgatgacgt gagcaccgcg ctcggcgaag gcgaggctca
      361 ccgaccggcc gatgcccgag cccgctcccg tgaccacgac gacgtcgcgc tccgggtcgg
      421 gggtcagcgc ccggccggcc tcgacgacgg ccacgtgctc ggccaccaac cgggcgacga
      481 ggtcggggtt cgaccgcggg atccagtggc cgccacccac ctcccgacgg acgaggttcg
      541 gggcgacgcg ctccatgccg tcgagcaggg ccggcgtcac gtaccggtcg ccgacgggga
      601 cgacgagctg gacgggcacc tgggtggtgg gcgggacctt cggcgccgtc cgcccgtaca
      661 tgttcgcccg gtagagctcg atgccgttga ccccgttggc cacgaggttc gggccgggcc
      721 accggtcgtc gacccgggca ccgtcgaccc gctcgagcag gagcgggaac ccgtcggcca
      781 agcgaccccg ctcccacacc ttcgccggga tcgacgtgcg gaacgcggcg atgtaccagg
      841 accggacggc ctggcgtcgg gcgtcgcgcc agcgcccgcg gccggggatc cggtgggcac
      901 ggatccaggc ggcgacgtgg tccaggcccg gccccgacat cgacgtgaag ctggcgaagc
      961 gccggccgac caccggatcg gtgaccgccg accaggactg gatcgacccc cagtcgtggg
     1021 cgacgaggtg gacgggccgg tcgggcgagg tcgctccgat cacggcgtcg aggtcggcga
     1081 tgagggcctc gatccggtag cccgcacgat cagccgggat ctccgaggcg ccggcaccgc
     1141 ggacgtcgta ggtgacgacg tggtgatcgg cggccagccg atcggccacc aggtcccaga
     1201 cggcatggtc gtcggggtag ccgtggacca agacgacggg gggacgacca gcctcgccgc
     1261 gctcctgcac ggcgagcgag agcccgccga agccggcgac gcgccgctcg cgcacctcgg
     1321 ccaccgacgc ggtgccgctc atcgggcgcc cgtcggggtg atgccccggt ccgcccagcg
     1381 gcgcacgtgc gggaggtcgt cgtcgagcca gaaggcgtcg tcgtcggtga tgaccaggat
     1441 ctcctcgaac ttggcaccgg cgcctcggag gccgaggtgc ggctccatcg cccagaggcc
     1501 ggcgacgggc gggtggccgc ttcgcggctc accggaccag aggggcgacc agcgctcgcg
     1561 acggccgacc gcgatggtcc gcacgagcga ccggacgtgg cggcgaccga agcgggccgg
     1621 ggtgagcccg atgccgggac ccgggtcgag cggctcgatg gcgtggccga tcacgtgggc
     1681 cgggtaggcg tggtggcggt tggcgtaccc ttgctccgca gcgaggcggt cgacggcctc
     1741 gtacacctcg cggaacgtcc gaccggcccg cacctgatcg acgatcaggg tccggtgcgc
     1801 ggccaagtcg tccatgatct gctcgacggc cgggttggca ccgcaggtgg cggtgaagcc
     1861 gatgtcggcg gtggcgccgc gcaccacggg cgcggcgtcg aggatgaagc ccatcccctc
     1921 ctccagccgc cggttggtgg gcaggaactg cagcggcacg cggaagtgcc agaaggcggc
     1981 ccggtcgccg aaccaggcga aggggaggtg gaaccagtcg tcgacgccgt cggcctccag
     2041 gcgacgtcgc agggcgagcg ccgcctgccg ctcggtcatg ccgggctcga gcgtggcggc
     2101 gacgtcggcc gcggcccggt acgcgagctg ctgcaccgcg cggaaggcgt cgagctggtc
     2161 gggtgtgggc gcgtggccgc gtcgggccgg cgtcggagga ggggtcgctg tcttcaccac
     2221 tgaccactgt gacatcaaac aatgtcatgg tcaaccgacc agcagacccc ctggtcagac
     2281 cccatcttgc caccgatctc ttgacaacct ctcgaagtgt gttaggcatt cctaacatca
     2341 cagcccgagg aacccaggaa ccgatgtcac ccgatcacaa cgtccccacc gagccgctca
     2401 gccccttcca aggaggcgcc gccgctcacc ccgctcgcca agcctggctc ctcgaggagc
     2461 tcgagcagtg gttcgccggc gacctgcccg acggccgtga ccgccgccgc atcgtcgccc
     2521 aggcgctgcg ctccgccacc gaagatgcgc accactggct ggaccagcgg ctcccctgcg
     2581 atcacctctt gcgagtcgac ctccacctct gcaacaaggc cggcccccgg cactcggtct
     2641 tcccgtccct gcccacgccg ctcggcctcc tgctgatctg cctggaccag tgccaggtcg
     2701 gcgacgaggt cgtcctcgtc caggagctgg ccaagtccgg ccgggtcgag gcccgggcca
     2761 cgtggccctt cggcatggac gaggccgaga ccctcacccg ccccgttcga gtcccggtgg
     2821 gcgccaacca ggcctgagga caccgctctc cgagccgcag caactccgac acgggaggca
     2881 gccgctcgga ccgggcgggc gtcgggaccc ggttcccccg acgcccgccc caacatcgca
     2941 ccaccggcgc gaccacgcgc cgccgcaacg ccgtgggagc ccgaacgggc gggccccgcg
     3001 cacggtctcc gagccgcagc aactccgaca cgggaggcag ccgctcggac cgggcgggtg
     3061 tcgggacccg gttcccccga cacccgcccc acagctcgcc gcctccaccg ccctcggacc
     3121 cggcgacggc gacgaggtgc ggtacggccc ggccgaagcg acatccagag gactcgtggc
     3181 cgtcgaccgc accaagctcg ccgagcgcct cgctgccatc accccgttcc gcaacagggc
     3241 gcccatggcc ccgagcacca cccgagccga cgtcgacgcc cacaccaccg ccttcgcggg
     3301 ggccgtcacc gcgatctggt gagacgtccg gtcagcgggc ggcgtcgggg agggggccgg
     3361 tccagggctc ggacggcttg cgcatcgagg cccagtacga ctgggtgaag gcgatctcct
     3421 tgtcccacgt cgacgcgccg gcctcgatct gctcggcgac gaggcccagc tccttgcggc
     3481 agcgagcgga ccacagggtc atcagtccgc ccccgaccac cgggagggcg agcagcgcca
     3541 gcatcgagta gccgcggtgg gcgagcagcg ggatgacgaa gaggtagggg aagacgacgg
     3601 ccacgaacac catcgggatg gtgaccgcgg ccgtgcggaa gcgctgcacc gctcgggcga
     3661 actcggggct gtcgaccgag cggcgctcgt cggcgaacac ggcctggaac ccgctcggcg
     3721 cgtgctcggc gtgcgccggg cgctcccacc c
//
LOCUS       DATMFS010000465         3040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_295746, whole genome shotgun sequence.
ACCESSION   DATMFS010000465 DATMFS010000000
VERSION     DATMFS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295746"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VNQ33_08775"
     CDS             complement(<1..1185)
                     /locus_tag="VNQ33_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98239.1"
                     /translation="MTAPTQDPPAPDPGAPPAVELAGIVKRFPGVVANRNVNLRVAPT
                     TIHAIVGENGAGKSTLMKILYGMQAPDEGTISVDGEQVSFKSPNDAIEAGIGMVHQHF
                     MLADNLTVIENIVLGYEPRRPFPLRFLVDTGAARERIVALGQRYGLAVDPDALVETLG
                     VGQRQRVEILKVLFRGATTLILDEPTAVLVPQEVDELFANLRDLKREGITILFISHKL
                     DEVLAAADDITVIRAGTTVATAKPADVTTKQLAELMVGSELPTPETRLSTVTDQVELS
                     VEGLGIAPIGLRPTLDDISFDIRRGEIVGIAGIEGNGQTELIAAILGLIDLNHGTVRL
                     GDEDITSWSTRKRRDAGIGYVPEDRHRHALLLDAPLWENEILGHQSKPPNAKGPWIDR
                     AGA"
     gene            complement(1258..2370)
                     /locus_tag="VNQ33_08780"
     CDS             complement(1258..2370)
                     /locus_tag="VNQ33_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWJ98240.1"
                     /translation="MKQRSSSKLLILLFAVFALAVASCASSDSDGGSDGGGSETAATC
                     QGTPGEKQEAADLGGSDDPDGSGKKVGLVFDIGGKDDNSFNESAYNGLLAAKDNMGVE
                     IKALEPNTDGSNREDLLRQLAEDGYGLIIGVGFNFADPLTTVAKDYPDTDFGIVDSVV
                     EEDNVASITFAEEQGSFLVGAIAAQSTQTGTVGFVGGVEQDLIKKFEAGFTAGVKEVK
                     SDADVEVKYISPDGDFSGFNDPAKGKTIASGLYDAGADVVFHASGGSGSGVFKAAVDA
                     DRLAIGVDSDQYFQVDEPEQKCMLSSMLKRVDVGVYDTIADYAGNGFKSGVQVFDLAN
                     GGIDYSQAGGQVKDPAQIDDLKQQIIDGDIEVPTKP"
     gene            2583..>3040
                     /locus_tag="VNQ33_08785"
     CDS             2583..>3040
                     /locus_tag="VNQ33_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ98241.1"
                     /translation="MSAPLTVPDPEDDPLGSRLLAAATEVFAEEGYAGARVAEIARRA
                     GVTTGAIYSRYRGKDELLASAIAAATAGEFDSLFSDHRFEGRMEDILRIAGAHLVERN
                     QSPNQRELSAPGMLLESFVAARHEPTVLAFLQARMNDRRDRLAEIVRGAKA"
BASE COUNT          416 a          986 c         1124 g          514 t
ORIGIN      
        1 ggcgccggcc cggtcgatcc acggcccctt ggcgttcggc ggcttgctct ggtggccgag
       61 gatctcgttc tcccagagcg gggcatcgag gaggagggcg tggcggtggc ggtcctcggg
      121 cacgtagccg atgccggcgt cgcgccgctt gcgggtggac cacgacgtga tgtcctcatc
      181 gcccagccgg acggtcccgt ggttgaggtc gatgagtccg aggatggcgg cgatgagctc
      241 ggtctgcccg ttgccctcga tcccggcgat gccgacgatc tcgccgcggc ggatgtcgaa
      301 cgagatgtcg tcgagcgtcg gccggaggcc gatgggggcg atgcccaggc cctcgaccga
      361 cagctcgact tggtcggtga ccgtcgacag ccgggtctcg ggcgtcggca gctcgctgcc
      421 caccatcagc tcggcgagct gcttcgtggt cacgtctgcc ggcttggccg tggcgaccgt
      481 ggtcccggcc cggatgacgg tgatgtcgtc ggcggcggcc aggacctcat cgagcttgtg
      541 cgagatgaag aggatggtga tgccctcgcg cttgaggtcg cgcaggttgg cgaacagctc
      601 gtcgacctcc tgggggacca gcacggcggt gggctcgtcg aggatgaggg tggtggcgcc
      661 gcggaagagg accttcagga tctcgacccg ctggcgctgg ccgacgccga gcgtctcgac
      721 gagggcatcg gggtcgacgg cgaggccgta gcgctggccg agcgccacga tgcgctcccg
      781 agccgcaccc gtgtcgacca ggaagcggag cgggaagggc cgtcgaggct cgtagcccaa
      841 gacgatgttc tcgatgacgg tgaggttgtc ggcgagcatg aagtgctggt gcaccatgcc
      901 gatgccggcc tcgatggcgt cgttggggct cttgaacgag acctgctcgc cgtcgacgga
      961 gatggtgccc tcgtcgggcg cctgcatccc gtagaggatc ttcatgaggg tgctcttgcc
     1021 ggcgccgttc tcgccgacga tcgcgtggat ggtggttggg gccacccgga ggttgacgtt
     1081 gcggttggcg acgacgcccg ggaaccgctt cacgatgccg gccagctcga cagctggcgg
     1141 tgcgcctgga tcaggggcgg ggggatcctg tgtgggagcg gtcacggaag gcctccagaa
     1201 cgccaagacc cgggaccggc cgaccggtcc cgggtctcga tcgggttcga ccggtgatca
     1261 gggcttggtg gggacctcga tgtccccgtc gatgatctgc tgcttcagat catcgatctg
     1321 cgccgggtcc ttcacctggc cgccggcctg cgagtagtcg atgccgccgt tggcgagatc
     1381 gaacacctgc acaccggact tgaagccgtt gccggcgtag tcggcgatgg tgtcgtagac
     1441 gcccacatcg acccgcttca gcatcgacga gagcatgcac ttctgctcgg gctcgtcgac
     1501 ctggaagtac tggtcggagt ccacgccgat ggcgaggcgg tccgcgtcga ccgccgcctt
     1561 gaacacgccg gagccggagc cgccggacgc gtggaagacg acgtcggcgc cggcgtcgta
     1621 gaggcccgag gcgatggtct tgcccttggc cgggtcgttg aagccggaga agtctccgtc
     1681 gggcgagatg tacttgacct cgacgtcggc gtcggacttg acctccttca cgccggcggt
     1741 gaagccggcc tcgaacttct tgatgaggtc ctgctccacg ccgccgacga agccgacggt
     1801 gccggtctgg gtcgactggg cggcgatggc gccgaccagg aacgagccct gctcctcggc
     1861 gaaggtgatc gaggcgacgt tgtcctcctc gacgaccgag tcgacgatgc cgaagtcggt
     1921 gtcggggtag tccttggcga cggtggtcag cgggtcggcg aagttgaagc cgacgccgat
     1981 gatgaggccg tagccgtcct cggcgagctg gcgcagcagg tcctcgcggt tggagccgtc
     2041 ggtgttgggc tcgagggcct tgatctcgac gcccatgttg tccttggcgg cgaggaggcc
     2101 gttgtaggcc gactcgttga acgagttgtc gtccttgccg ccgatgtcga acacgaggcc
     2161 gaccttcttg cccgagccgt cggggtcgtc gctgccgccc aggtcggcgg cctcctgctt
     2221 ctcgccgggc gtgccctggc aggtggccgc ggtttctgaa ccgccgccgt ccgatccacc
     2281 atccgaatcg ctgctcgcgc agctggccac ggccagcgcg aagacggcga agaggaggat
     2341 cagcagcttg ctgctcgatc gttgcttcac gagagatctc ccttgtcgga ccctgggcgc
     2401 ccagtcgcgt cgggtgggat cagcaccgta ctgcgcgctt tcggggcgtg cgctgggttc
     2461 tgcgccggta cggacgaccg catcgagcgc tcccggtcga gggtcggtgc gtcctccgct
     2521 ggcggagcgc ggcccgcagg tgcgctcgac agatccggac gggcgccggt accgtcgtcg
     2581 tggtgtctgc cccgctgacg gtgcccgatc ccgaggacga tccgctcggc tcgcggctgc
     2641 tcgcagcggc cacggaggtc ttcgcggagg agggctacgc cggcgcccgc gtcgcggaga
     2701 tcgcccgccg ggccggggtc accaccggcg ccatctactc ccggtaccga ggcaaggacg
     2761 agctgctcgc gtcggccatc gcggcggcca cggcgggcga gttcgactcc ttgttctcgg
     2821 accaccgctt cgaaggccgg atggaggaca tcctccggat cgccggcgcc cacctggtcg
     2881 aacggaacca gagccccaac cagcgcgagc tgtcggcgcc cgggatgctg ctcgagtcgt
     2941 tcgtcgccgc ccgccacgag cccaccgtct tggcgttcct ccaggcgcgg atgaacgacc
     3001 ggcgcgaccg cctggccgag atcgtccggg gggccaaggc
//
LOCUS       DATMFS010000466         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_319309, whole genome shotgun sequence.
ACCESSION   DATMFS010000466 DATMFS010000000
VERSION     DATMFS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319309"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VNQ33_08790"
     CDS             <1..758
                     /locus_tag="VNQ33_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWJ98242.1"
                     /translation="QTAAACAKLGVRCELILTRLVDRTDPFHEQSGNRLLDTVLGARV
                     HECADSDEVVERTAALHDEAAEEGRTIFTVVGGGSDATGALGYVNATLELATQAEEQG
                     VDIGRIVTAASTAGTAAGLVVGTDLAGLDATVDIACVLASKEETSPLVNRLSVEVVAL
                     LGIAGGSSDLRITDATLGAGYGIPSPATLDAVELLARTEGVLLDPVYTGKAFEHLLAE
                     VAAGQLDPERDVVFVHTGGSPGLFAYTPAFTAR"
     gene            810..1103
                     /locus_tag="VNQ33_08795"
     CDS             810..1103
                     /locus_tag="VNQ33_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98243.1"
                     /translation="MQTAIMPNITIRDVPDPVHRKLVARAERNHQSLQQYLLEVITEM
                     TEKLTMDEWLDRAAALRATMPPITADTDALLDAALAERQAKLARLAGISLADE"
     gene            1115..1504
                     /locus_tag="VNQ33_08800"
     CDS             1115..1504
                     /locus_tag="VNQ33_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ98244.1"
                     /translation="MVLDSSALLPLVTNRPGRRRVIELAMGVDILIPHVADVEVLMSL
                     RDLWLTGQIDQLHLTGAAAELEAMPAPRFGMLGLHQRVLQLRAHLSTFDATYVALAES
                     LELELVTYDRAQADAPGIRCDVILLED"
     gene            complement(1517..>2150)
                     /locus_tag="VNQ33_08805"
     CDS             complement(1517..>2150)
                     /locus_tag="VNQ33_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWJ98245.1"
                     /translation="VDQICAMPNVAGDLSVFDCAGVADGSASAIVVRAEDAYRYTDKP
                     IFVKALSFIAGNGSGLVDPGYDYTWFPEIAACAADAYAQAGITDPVSRIAMAEVHDCF
                     TPTELVLMEDLGFSERGMAWKDIESGRFDLNGELPVNPDGGLKSFGHPVGASGLRMMF
                     EAYLQLRREAPEDRTISSISGDRNLALTHNLGGYPGEMVSFVSILGADKD"
BASE COUNT          325 a          770 c          732 g          323 t
ORIGIN      
        1 gccagaccgc ggccgcctgc gccaagctgg gggtgcgctg cgagctgatc ctgacccgcc
       61 tggtcgaccg caccgacccg ttccacgagc agtccggcaa ccgcctgctc gacacggtcc
      121 tcggcgcccg agtccacgag tgcgccgatt ccgacgaggt cgtcgagcgc accgccgccc
      181 tccacgacga ggccgccgag gaaggccgca cgatcttcac cgtggtgggc ggcggctccg
      241 acgcgacggg tgcgctgggc tacgtgaacg ccaccctcga gctggccact caggcggagg
      301 agcagggcgt cgacatcggc cgcatcgtca cggctgccag cacggcgggg accgccgccg
      361 gtctggtggt cggcaccgac ctcgccggcc tcgatgccac cgtcgacatc gcctgcgtgc
      421 tcgcctcgaa ggaggagacc tcgccgctgg tgaaccgcct cagcgtggag gtggtcgccc
      481 tgctcggcat cgccgggggc agttcggacc tccggatcac cgacgccacg ctgggcgccg
      541 ggtacgggat cccgagcccg gccacgctcg acgcggtcga gctcctggcc cgcaccgaag
      601 gcgtcctcct cgaccccgtg tacaccggca aggcgttcga gcacctcctc gccgaggtgg
      661 ccgccggtca gctcgatccc gagcgcgatg tggtgttcgt ccacaccggc ggaagcccgg
      721 gtctcttcgc ctacaccccc gccttcaccg cccgctgacg ccactcatca ggcgttgaac
      781 ccaactccgt tgcgcgcgac cgtacatgca tgcaaactgc aatcatgccg aacatcacca
      841 ttcgagacgt cccagatccg gtgcaccgca agctcgtggc ccgggccgag cgcaaccacc
      901 agagcctcca gcagtacctc ttggaggtga tcaccgagat gaccgagaag ctgacgatgg
      961 acgagtggct cgaccgtgcc gccgcgctgc gagcgaccat gccgccgatc actgcggaca
     1021 cggacgcgct cttggacgcg gcgctggccg agcggcaggc gaagctcgcc cgtctggccg
     1081 gcatctccct tgccgacgaa tgatgcgaac ctcgttggtc cttgactcgt cggccctcct
     1141 gcccctggtc accaaccgtc cgggtcgacg acgggtcatc gagctggcca tgggtgtcga
     1201 catcctcatc ccgcacgtcg cggatgtcga ggtgctcatg tcgttgcgcg acctctggct
     1261 caccgggcag atcgatcagc tgcacctgac gggggcggcc gcggagctgg aggcgatgcc
     1321 ggccccgcgc ttcgggatgc tcggtctcca ccagcgggtg ctgcagctgc gcgcccactt
     1381 gagcaccttc gacgcgacct acgtcgcgct ggccgagtcg ctcgaactgg agctggtcac
     1441 ctacgaccgc gcccaagccg acgccccggg aatccggtgc gacgtgatcc tgctcgagga
     1501 ctgagccccg gtcgggtcag tccttgtcgg cgccgaggat gctgacgaag ctgaccatct
     1561 cgccggggta gccgccgagg ttgtgggtca gggccaggtt gcggtcgccg ctgatggacg
     1621 agatcgtgcg gtcctccggc gcttcgcggc ggagctggag gtacgcctcg aacatcatcc
     1681 ggaggcccga cgcgccgacc gggtggccga agctcttgag cccgccgtca gggttgacgg
     1741 gcagctcgcc gttcaggtcg aagcggcccg actcgatgtc cttccacgcc atgccccgct
     1801 cggagaaccc gaggtcctcc atgagcacca gctcggtggg ggtgaagcag tcgtggacct
     1861 cggccatggc gatgcggctg accggatcgg tgatgccggc ctgggcgtag gcgtcggcgg
     1921 cgcaggcggc gatctccggg aaccacgtgt agtcgtagcc ggggtcgacc aggcccgagc
     1981 cgttgccggc gatgaagctc agcgccttca cgaagatcgg cttgtcggtg tagcggtagg
     2041 cgtcctcggc ccgcacgacg atcgccgacg cggagccgtc ggccacgccg gcgcagtcga
     2101 acaccgacag gtcgccagcg acgttgggca tggcgcagat ctggtcgacg
//
LOCUS       DATMFS010000467         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_202206, whole genome shotgun sequence.
ACCESSION   DATMFS010000467 DATMFS010000000
VERSION     DATMFS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202206"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="VNQ33_08810"
     CDS             complement(<1..2076)
                     /locus_tag="VNQ33_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWJ98246.1"
                     /translation="MSDSTLFAAAAARVAGGADAHAEAAALVGAMTEDERRWCLDGDH
                     PFWGDLSEMGQGGYHKRTFPAARVERLGIAGFAFSDGPRGVVIGPATAFPVSMARGAT
                     WDVDLEERIGEAIGVELRAVGATLYGGVCVNVLRHPAWGRAQETYGEDPVHVGELGAA
                     LTRGVQRHAMACVKHFACNSMENTRFAVDVTIDETALHEVYLPHFRRIVDEGVASVMS
                     AYNAVNGEWCGQSRMLLNDVLRERWGFDGFVISDWIFGLRDAGASVHAGLDVEMPYRM
                     IRAFGLDGDLAAGACTEDEIAAAATRVVATLLRFHPVLDQPRPPIDVLAQPTHRALAR
                     EAAAKALVLLRNEPVEGTPVLPLDAGSLTSVAVVGRLAAVRNLGDGGSSDVWAPSAVT
                     PLDGLRAALPAVEVRHSDGDVSVAAGSDVAIVVVGYTKADEGEFIGDSGTAHLASLMP
                     AADEPEVVAAYEATRAAEVRDFQPPPDAGGAGGFGFAKGGDRESLRLRPDDEELIAAV
                     AAANPRVVVCLVAGSAVLIDRWDRSVPAVVQSWYAGMEGGHALADVLLGRVQPSGRLP
                     FSVPADPDHLPSFDRDAVEATYDGWHGYWKLAADGNEAAYPFGFGLSYTDLSLLGTEA
                     AQHGDEIVATTVAVNAGTRPGVEVVQAYASAGDGPAKLVGFARVELDAGERKVSEVRF
                     PIARLARWDE"
     gene            complement(2073..>2260)
                     /locus_tag="VNQ33_08815"
     CDS             complement(2073..>2260)
                     /locus_tag="VNQ33_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98247.1"
                     /translation="LTAACREAGLEVADPDGAFVRAVASAEPAWPSLRDAVRPHEVVD
                     AIYRSAAEGGTRMELGA"
BASE COUNT          313 a          857 c          818 g          272 t
ORIGIN      
        1 ctcgtcccac cgggccagcc gggcgatcgg gaaccggacc tcggagacct tgcgctcgcc
       61 ggcgtccagc tcgacgcgcg cgaagccgac gagcttcgcc gggccgtcac ccgccgacgc
      121 gtaggcctgg acgacctcga cgcccggccg cgtcccggcg ttcaccgcga cggtcgtggc
      181 cacgatctcg tcaccgtgct gcgccgcttc cgtccccagc agcgacagat cggtgtagct
      241 gagcccgaac ccgaacgggt aggccgcctc gttcccgtcg gcggccagct tccagtaccc
      301 gtgccacccg tcgtaggtcg cctcgacggc atcgcggtcg aaagacggca ggtggtccgg
      361 gtcagccggc accgagaacg ggaggcggcc cgacggctgg acccggccga gcagcacgtc
      421 ggccagcgcg tggccgccct ccatcccggc gtaccaggac tgcaccaccg ccggcacgga
      481 acggtcccac cggtcgatca gcacggcgct gccggccacg aggcacacga cgacccgggg
      541 gttggcggcg gcgacggcgg cgatgagctc ctcgtcgtcg gggcgaaggc ggagggactc
      601 gcggtcgccg cccttggcga agccgaaccc gcccgctcca ccggcgtcgg gcggaggctg
      661 gaagtcgcgg acctcggcgg ctcgggtcgc ctcgtaggcc gccaccacct cgggctcgtc
      721 ggccgccggc atgagcgagg ccaggtgggc cgtgccgctg tcgccgatga actcgccctc
      781 gtccgccttc gtgtacccga ccacgacgat ggcgacgtcg gacccggctg cgaccgacac
      841 gtcgccatcg ctgtggcgca cctcgacagc gggcagcgcg gcccgcagcc cgtcgagcgg
      901 cgtcaccgcg ctcggcgccc acacgtcgct cgacccgccg tcgcccaggt tgcgcacggc
      961 ggcgaggcgg ccgaccacgg ccaccgacgt gagcgatccg gcgtcgagcg ggagcaccgg
     1021 cgtgccctcg accggctcgt tgcgcagcag gacgagggcc ttggccgccg cttcccgggc
     1081 gagggcccgg tgggtcggct gcgccagcac gtcgatcggc gggcggggct ggtcgagcac
     1141 cgggtggaac cgcaggaggg tggccacgac gcgggtcgcc gcggcggcga tctcgtcttc
     1201 ggtgcacgcg ccagccgcca ggtcgccgtc gaggccgaag gcgcggatca tccggtacgg
     1261 catctcgacg tcgagaccgg cgtgcaccga cgcaccggcg tcgcgcaggc cgaagatcca
     1321 gtcggagatc acgaacccgt cgaagcccca ccgctcccgg aggacgtcgt tgaggagcat
     1381 ccggctctgg ccgcaccact cgccgttcac cgcgttgtac gcgctcatca ccgacgccac
     1441 gccctcgtcg acgatccgcc ggaagtgcgg caggtagacc tcgtgcagcg ccgtctcgtc
     1501 gatcgtgacg tcgacagcga aacgggtgtt ctccatcgag ttgcaggcga agtgcttcac
     1561 gcaggccatg gcgtggcgct gcacacctcg ggtcagggcc gcgcccagct cgccgacgtg
     1621 gaccggatcc tcgccgtagg tctcttgggc acggccccag gcgggatgcc gcagcacgtt
     1681 gacgcacacg ccgccgtaga gggtggcgcc cacggcccgc agctcgacac cgatcgcctc
     1741 gccgatgcgc tcctcgaggt cgacgtccca ggtggcaccc cgcgccattg acacggggaa
     1801 ggcggtggcg gggccgatca ccacaccacg agggccgtcg gagaaggcga agccggcgat
     1861 gccgaggcgc tcgacgcggg ccgccgggaa ggtgcgcttg tggtagccgc cctggcccat
     1921 ctcgctcagg tcgccccaga acggatggtc gccgtcgagg caccagcgcc gctcgtcctc
     1981 cgtcatcgca ccgacgaggg ccgccgcctc ggcgtgggcg tcggcgccgc cggccacccg
     2041 cgctgccgcg gcggcgaaga gggtcgagtc gctcatgcgc ccagctccat ccgggtcccg
     2101 ccctcggcag ccgagcggta gatggcgtcg acgacctcgt ggggccgcac ggcgtctcgg
     2161 agcgaaggcc aagccggctc ggcgctggcg accgcccgca cgaacgcgcc gtcggggtcg
     2221 gccacctcca ggccggcctc gcggcacgcg gcggtgagcg
//
LOCUS       DATMFS010000468         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_319459, whole genome shotgun sequence.
ACCESSION   DATMFS010000468 DATMFS010000000
VERSION     DATMFS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319459"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VNQ33_08820"
     CDS             <1..1623
                     /locus_tag="VNQ33_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWJ98248.1"
                     /translation="QAFAMSVAQPMVIADPAQVEHPAATSRALASILGLDAADIEVQL
                     TSKYRYRIIAKSVSQADAEAIKDKLDDGELAGISLENEYQRSTPSDDLAIGVIGHALA
                     EGETTPDGKTGGVSGVEAEYDRQLQGKPGKLYYEQDVWGTPIAGGERKLDKAVQGTDL
                     YLTLDQAMQYEAEKAMADQVLATGAQSGQAVIMRPSTGEILAMASVAKGEDGTVSNTR
                     DNRSVTSVFEPGSVNKMITVAGAIEEGVVTPETILNVPDHLQLYDREFTDSHPHPASD
                     WSVTDIVATSSNIGTIKLAQELGKARVDRYLREFGFGTEPGLGFPGETAGIMKDLDDW
                     SGVDIGAMPIGQGVAVSALQMLDAYNVVANDGVFVAPKLVASTDSGRGQVPTEPSASH
                     RVVSKDTAAAMQAMLAKVVSDGTGGLAAVPGYTSAGKTGTARIPQGIDKEDGYLNGRG
                     RYEYQASFVGMVTGADLSIIVTLEDPRTSIYGGDVAAPVFAHLAASALRRYEVPPPAL
                     LGDMKHDVPELSASAKDVDGEDVAGATATAQG"
     gene            1598..>2230
                     /locus_tag="VNQ33_08825"
     CDS             1598..>2230
                     /locus_tag="VNQ33_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWJ98249.1"
                     /translation="MPPPLRKADRVLLPDLVAAATGRGSLDALDVVGDLDVDVRSVTH
                     DSRHVVPGTLFCCVPGAAFDGHDFAAGAVADGAVALLGERRLDLDVPQLVTPSVRAAM
                     GPVAAALVGFPSDTLQLAGITGTNGKTTVAHLIAAMAQAAGRSCGVIGTLTGARTTPE
                     APELQAQLAAMRDAGGDLVAMEVSSHALDLHRIDGTRFRVAVFTNLSQDHL"
BASE COUNT          345 a          844 c          746 g          295 t
ORIGIN      
        1 caggccttcg ccatgtcggt ggcccagccc atggtcatcg ccgatcccgc ccaggtggag
       61 caccctgccg ccacctcgcg ggcgctcgcc tcgatcctcg gcctcgacgc cgccgacatc
      121 gaggtccagc tcaccagcaa gtaccgctac cggatcatcg ccaagtcggt gtcgcaggct
      181 gacgccgagg ccatcaagga caagctcgac gacggcgagc tggcgggcat ctcgctcgag
      241 aacgagtacc agcgcagcac gcccagcgat gacctggcga tcggcgtcat cggtcacgcc
      301 ctggccgagg gcgagacgac gcccgatggc aagaccggcg gcgtttcggg ggtcgaggcc
      361 gaatacgacc ggcagctcca gggcaagccc gggaagctgt actacgaaca ggacgtctgg
      421 gggaccccga tcgccggcgg cgagcgcaag ctcgacaagg cggtccaggg caccgacctc
      481 tacctgacgc tggaccaagc catgcagtac gaggcggaga aggccatggc cgaccaggtc
      541 ctcgccaccg gcgcccagag cggccaggcg gtgatcatgc ggccgagcac cggtgagatc
      601 ctggccatgg cgagcgtcgc caaaggcgag gacggcacgg tctccaacac ccgggacaac
      661 cgctccgtca cctccgtgtt cgagccgggt tcggtcaaca agatgatcac cgtcgccggg
      721 gccatcgagg agggcgtggt cacgcccgag accatcttga acgtgcccga ccacctccag
      781 ctctacgacc gggagttcac cgacagccac ccgcacccgg cgtcggactg gtcggtcacc
      841 gacatcgtgg cgacgtcgtc gaacatcggc acgatcaagc tcgcccagga gctcggcaag
      901 gccagggtgg accgctacct ccgcgagttc gggttcggca ccgagcccgg cctcgggttc
      961 cccggtgaga cggccgggat catgaaggac ctcgatgact ggtccggcgt cgacatcggc
     1021 gccatgccca tcggccaggg cgtcgccgtg agcgccctcc agatgctcga cgcctacaac
     1081 gtcgtggcca acgacggcgt gttcgtcgct ccgaagctgg tggcctccac cgactcgggc
     1141 cggggccagg tgcccaccga gccgtccgcc tcccaccggg tcgtctccaa ggacacggcg
     1201 gcggccatgc aggcgatgct cgccaaggtc gtgtccgacg gcaccggcgg gctcgccgcc
     1261 gtgcccggct acacctccgc cggcaagacc ggcaccgccc gcatccccca gggcatcgac
     1321 aaggaggacg gctacctcaa cgggcggggc cgctacgagt accaggccag cttcgtgggc
     1381 atggtcaccg gcgccgacct gtcgatcatc gtgacgctcg aagacccccg cacctcgatc
     1441 tacggcggtg acgtggccgc tccggtcttc gcccacttgg cggcgtcggc cctgcgccgc
     1501 tacgaggtcc cgccgcctgc cctcctgggc gacatgaagc acgacgtgcc ggagctgtcc
     1561 gcttcggcga aggacgtgga cggcgaggat gtcgctggtg ccaccgccac tgcgcaaggc
     1621 tgaccgggtg ctcctcccag atctggtcgc ggccgccacc ggccggggct ccctggacgc
     1681 cctcgacgtg gtcggtgacc tcgacgtcga cgtccggtcc gtcacccacg actcccgcca
     1741 cgtggtgccg gggacgctgt tctgctgcgt ccccggcgcc gcgttcgacg ggcacgactt
     1801 cgccgccggc gccgtcgccg acggggccgt ggccctgctg ggcgagcgcc ggctcgacct
     1861 cgacgtgccc cagctggtga cgccatcggt gcgggccgcc atggggccgg tggccgccgc
     1921 cctcgtcggc ttcccgtccg acaccctcca gctcgccggc atcaccggca ccaacggcaa
     1981 gaccacggtc gcccacctca tcgcggcgat ggcccaggcc gcgggccggt cctgcggcgt
     2041 gatcgggacg ctcaccggcg cccgcaccac gcccgaggcg cccgagctgc aggcccagct
     2101 ggccgccatg cgcgacgccg ggggcgacct ggtggccatg gaggtgtcgt cccacgccct
     2161 ggacctccac cggatcgacg gcacgcgctt tcgggtggcc gtgttcacca acctcagcca
     2221 ggaccacctc
//
LOCUS       DATMFS010000469         3321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_132459, whole genome shotgun sequence.
ACCESSION   DATMFS010000469 DATMFS010000000
VERSION     DATMFS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132459"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VNQ33_08830"
     CDS             <1..239
                     /locus_tag="VNQ33_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98250.1"
                     /translation="RTPEDRVRHDAVRAAARFVRARRGDGPPGTVPATTTHQAVLAAE
                     LHPWFLTTDPSTGELRVAEPEPAVIRAAVDAARP"
     gene            415..1593
                     /locus_tag="VNQ33_08835"
     CDS             415..1593
                     /locus_tag="VNQ33_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ98251.1"
                     /translation="MADAPPSEPQSTKPITVVMIDDHEMLLESLRRLVGSQPDMAVIG
                     SGVTVAEGLAVVAAQQPDVVVLDYRLPDGDGAAAASAITGRHPQTRVVLLTGSDPDTA
                     VFEAARAGCAGFVDKARAPSELVRVIRSVHEGNLELPGHLVGQLPTPDELRVHYQPIV
                     KLTDGTIVGFEALVRWEHPVRGMVPPIDFIPLAERTSLIIDIDEVVRQHACAQAASWQ
                     ARFPREEPLFMSVNLSGRELQLPDLPQRVQAALDETGLDAASLVIEVTETFLIDTGAQ
                     GTTVLDELKAIGVRIALDDFGTAYSSLGYLQRFPIDIIKLDKSFTDELPDGERGLRLI
                     DAVGRLARDMGATVEAEGIESQDQADVLVRLGWELGQGYLYSRPEPAEAMTRLLDAQS
                     "
     gene            complement(1602..>3321)
                     /locus_tag="VNQ33_08840"
     CDS             complement(1602..>3321)
                     /locus_tag="VNQ33_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ98252.1"
                     /translation="DIPFVVLSGTIGEEAAVRALKAGAADVVLKGNMSRLASVARREL
                     TAATERRIRRAAEAALRESEALKTAILQSVFDAVISIDAEGRIIEFNRAAEQVFGFTR
                     SEVLGEVMVDLLVPPELRNQHREGFALYLATRQATILNRRLTMSALRRDGTEFPVELA
                     VVALDLPGETMFTANLRDITGQERMQRELAQSQRLESLGQLAGGVAHDFNNLLAVIMN
                     YAEFVRSEVEGNEQASSDVAAIVQAAEQAAQLTRQLLAFGRREVVHPRLVELNEVLAQ
                     VQQLLARTIGDYLQISSRTQDDLSSVFIDPGQVEQVLLNLALNARDAMPGGGELTFRT
                     EDVYLDDLAVQHFSELTPGRYVRLRVSDSGMGMSKATLERAFEPFFTTKPKGEGSGLG
                     LATCYGIVRQAGGDIHLYSEPSQGTTVSIYLPAAEDETGAPTVDSAQSTYHPAGGTEV
                     ILVVEDEPAFREMARRILEAAGYTALTAADAPEGLEAVGIHGARIDLILTDVVMPGHS
                     GIEMAAQLRATHPEIPLIFMSGYAESIVLGTTEVAPDELLAKPFNQTTLLERVRTVLD
                     RRGPRG"
BASE COUNT          492 a         1196 c         1160 g          473 t
ORIGIN      
        1 accgcacccc cgaggaccgc gtccgccacg acgccgtgcg cgccgcggca cgcttcgttc
       61 gagcgcggag gggagacggg cctcccggca cggtcccggc gacgacgacc caccaggcgg
      121 tcctcgccgc tgagctgcac ccgtggttcc tcaccaccga tccgtccacc ggcgagctgc
      181 gcgtggccga gcccgaacca gccgtgatcc gagccgcggt cgacgcagcc cgcccctgac
      241 cgcggcacct cgtggtgaac ggccgccgcc acgcagcgtg atcgaggact accctcgtcc
      301 ggcgaggggc ggcccgaggc gaccgagggt ctgagagcgg gcacgtgaag cgaccaacga
      361 gcacaggcaa cccggcacag ccgacggacg gccccgccac gggagcggtg caccatggct
      421 gacgcgcccc cgtcggagcc tcagagcacc aagccgatca ccgtcgtgat gatcgacgac
      481 cacgagatgc tcctcgagag cctgcggcgt ctggtgggct cgcaacccga catggcggtg
      541 atcgggtcgg gggtcaccgt cgccgagggt ctggccgtgg tcgcggcgca gcagccagat
      601 gtcgtcgtgc tcgactaccg cctgcccgac ggtgacgggg ccgcggccgc gagcgccatc
      661 accggtcggc acccgcagac ccgcgtggtc ctgctgaccg gatccgatcc cgacaccgcg
      721 gtgttcgaag cggcgagagc cggctgcgct gggttcgtcg acaaggcgcg agcgccgagc
      781 gagctggtcc gggtgatccg cagcgtgcac gaggggaacc tcgagcttcc cggccacctg
      841 gtcggccagc tccccacccc cgacgagctg cgcgtgcact accagcccat cgtgaagctc
      901 accgacggca ccatcgtcgg cttcgaggcc ctggtccggt gggagcaccc ggtccgcggg
      961 atggtgccgc cgatcgactt catccccctc gccgagcgca cgtcgctcat catcgacatc
     1021 gacgaggtgg tccgccagca cgcgtgcgcc caggccgcga gctggcaggc ccgcttcccc
     1081 cgcgaggagc cgctgttcat gagcgtgaac ctgtcgggtc gggagctcca gctgcccgac
     1141 ctcccccagc gggtccaggc tgcgctcgac gagacggggc tcgacgccgc ctcgctcgtg
     1201 atcgaggtca ccgagacgtt cctcatcgac acgggggccc agggcacgac ggtgctcgac
     1261 gagctcaagg ccatcggggt gcgcatcgcc ctcgacgact tcggtaccgc ctactcgtcg
     1321 ctcggctacc tccagcggtt cccgatcgac atcatcaagc tcgacaagtc gttcaccgac
     1381 gagctgcctg acggggagcg ggggctccgg ctgatcgacg ccgtcggccg actggcgcgg
     1441 gacatgggtg cgaccgtcga ggccgagggg atcgagagcc aggaccaggc ggacgtgctc
     1501 gtccgcctgg ggtgggagct cggccagggc tacctgtact cccgccccga gcccgccgag
     1561 gccatgaccc ggttgctgga cgcgcagagc tgacggaccg gtcagcctct cgggccgcga
     1621 cggtccagca cggtgcggac ccgctcgagg agcgtcgtct ggttgaacgg cttggcgagc
     1681 agctcgtcgg gcgcgacctc ggtggtgccc agcacgatcg actcggcgta gcccgacatg
     1741 aagatcagcg ggatctccgg gtgcgtcgca cggagctgcg ccgccatctc gatgcccgag
     1801 tgtcccggca tcacgacatc cgtcaagatc aggtcgatcc gggccccgtg gatcccgacg
     1861 gcctcgaggc cctccggcgc gtcggccgcg gtgagggcgg tgtagcccgc agcctccagg
     1921 atccggcggg ccatctcgcg gaaggccggc tcgtcctcca cgaccaagat gacctcggta
     1981 ccgcccgccg ggtggtaggt cgactgcgcc gagtccacgg tcggggctcc ggtctcgtcc
     2041 tcggccgccg gcaggtagat cgacaccgtg gtgccctggc tcggctccga gtacaggtgg
     2101 atgtcaccac cggcttgccg cacgatcccg tagcaggtgg ccaggccgag gccgctgccc
     2161 tcccccttgg gcttggtggt gaagaacggc tcgaacgccc gctcgagcgt ggccttggac
     2221 atccccatcc cgctgtcgga gacgcgcagg cgcacgtagc ggcccggtgt cagctcgctg
     2281 aagtgctgga ccgccaggtc gtcgaggtac acgtcctcgg tgcggaacgt cagctcgccg
     2341 ccgccgggca tggcatcgcg cgcgttgagg gccaggttga gcaggacctg ctcgacctga
     2401 cccgggtcga tgaacacgga cgagaggtcg tcttgggtcc gagacgagat ctggaggtag
     2461 tcgccgatgg tccgggccag gagctgctgg acctgggcca ggacctcgtt gagctcgacc
     2521 agccgcgggt gcacgacctc acggcggccg aacgccagga gctgccgggt cagctgggca
     2581 gcctgctccg cggcttggac gatggccgcg acgtcgctgc tggcctgctc gttgccctcg
     2641 acctcgctcc gcacgaactc ggcgtagttc atgatgacgg cgagcaggtt gttgaagtcg
     2701 tgggccaccc ctcccgcaag ctggccgagg ctctccaggc gctgggactg ggccagctcg
     2761 cgctgcatgc gctcttggcc ggtgatgtcg cgcaggttcg cggtgaacat cgtctcgccg
     2821 ggaaggtcga gcgcgaccac ggccagctcc acggggaact ccgtcccgtc ccggcgcagg
     2881 gcgctcatgg tcaagcggcg gttcaagatc gtggcctggc gggtggcgag gtagagggcg
     2941 aacccctcgc ggtgctggtt ccgcagctcg ggcggcacca ggaggtcgac catcacctcg
     3001 ccgagcacct ccgaccgcgt gaacccgaag acctgctcgg cagcgcggtt gaactcgatg
     3061 atccggcctt cggcatcgat cgagatgacc gcgtcgaaca ccgactgcaa gatggcggtc
     3121 ttgagcgcct cgctctcgcg cagcgcggcc tccgcggcgc ggcggatgcg ccgctcggtc
     3181 gcggccgtca gctcccgcct cgccaccgag gcaaggcggg acatgttgcc cttcaagacg
     3241 acgtcggccg cccccgcctt caacgcccgg accgccgctt cttcgccgat ggtccccgac
     3301 agcaccacga acgggatgtc c
//
LOCUS       DATMFS010000470         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_177103, whole genome shotgun sequence.
ACCESSION   DATMFS010000470 DATMFS010000000
VERSION     DATMFS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177103"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VNQ33_08845"
     CDS             complement(<1..684)
                     /locus_tag="VNQ33_08845"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWJ98253.1"
                     /translation="MDIRVSVIGAGSWGTTVAALAAHNAPTVLWSRRATLAEQIDQEH
                     ENGDYLAGYRLPTQLRATADLEEAVRQADLLVMGVPSQGFRGALESVKPYLRPWVPVI
                     SLSKGLELGTKLRMSQLIEEVLPGHPVGILTGPNLAKEILAGHAAASVVAMDDHNIAR
                     AVQDIFQAELFRVYTNEDIIGCEMGGVLKNVIAIASGMADGLGAGDNTRAAVITRGLS
                     EVTRMGVALG"
     gene            786..1370
                     /gene="cofC"
                     /locus_tag="VNQ33_08850"
     CDS             786..1370
                     /gene="cofC"
                     /locus_tag="VNQ33_08850"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWJ98254.1"
                     /translation="MQAAVVIPVKAFHAAKVRLADALSPEARAVLAREMATRVVRAAA
                     PLPVLVVCDDEAVRSWAVDVGAEPHWTPGLGLIGAVEAGVDAAAAAGADRVLVAHADL
                     PLATGLGHVVGESGVVIVPDRWNDGTNVIAVPATAGFRFAYGPGSAERHQAEAHRLGL
                     AVQIVDDHVLGWDVDVPGDLLLPSGEDLAAGIPS"
     gene            1367..2116
                     /locus_tag="VNQ33_08855"
     CDS             1367..2116
                     /locus_tag="VNQ33_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWJ98255.1"
                     /translation="MSGPASRDLPVPERALAVGAHPDDIEFGAGATLARWAAAGCEVS
                     LLVCTDGSKGTWDPDADLTALVATRQEEARAAAQALGATGEVVFLGVTDGELANDRAT
                     RSELARWIRILRPGVLLGHDPWKRYRLHPDHRAGGFLVCDALVAARDPHFFPEHGTAH
                     HRPDALLLFEADEPDHAEHADEDGLAAKVAALESHRSQYESTMFITDDPEAGAAEQAR
                     FRAEVRDRLVEAGDWAGIALAERFRLLPTDR"
     gene            complement(2259..>2595)
                     /locus_tag="VNQ33_08860"
     CDS             complement(2259..>2595)
                     /locus_tag="VNQ33_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWJ98256.1"
                     /translation="RFSWGVQLGTDGDDLQWPSADLVRYDADRKASETVSFGRDRIVG
                     EAVFVPRSAEAAEDDGWYLALVNDVPDDTTDLVVLDAAAPTEGPVATVHLPARVPLGF
                     HGNWIPTGQ"
BASE COUNT          324 a          983 c          948 g          340 t
ORIGIN      
        1 cccgagggcg acccccatcc gggtcacctc ggagaggccg cgggtgatga cggcggcccg
       61 ggtgttgtca ccggcgccga ggccgtccgc catgcccgac gcgatggcga tgacgttctt
      121 cagcacgccg cccatctcgc agccgatgat gtcctcgttc gtgtacaccc ggaacagctc
      181 ggcctggaag atgtcctgga cggcccgggc gatgttgtgg tcgtccatgg ccacgacgct
      241 ggccgcggcg tggccggcga ggatctcctt cgccaggttc ggcccggtga ggatgccgac
      301 ggggtggccg gggagcacct cctcgatgag ctggctcatc cgcagcttgg tcccgagctc
      361 gagccccttc gagagcgaga tgaccgggac ccacgggcgc aggtagggct tcaccgactc
      421 cagcgcaccg cggaacccct gcgagggcac gcccatcacg aggaggtcgg cctgccgcac
      481 ggcctcctcc aggtcagcgg tggcccgcag ctgggtgggg agccggtacc cggccaggta
      541 gtcgccgttc tcgtgctcct ggtcgatctg ctcggcgagc gtggcccgcc gcgaccagag
      601 gaccgtgggc gcgttgtggg cggcgagggc ggcgaccgtg gtgccccacg atccggcgcc
      661 gatgacggag acccggatgt ccatgggccg ggacactagg tgggatcagc gccgggtgtg
      721 acgggtggca cggggctgac ggcggccggg cacgcagcgc ccggggcgcc ggtagcgtag
      781 cgcccgtgca ggccgccgtc gtcatcccgg tgaaggcgtt ccacgccgcc aaggtgcgcc
      841 tggccgacgc cctgtccccc gaggcgcggg ccgtgctggc gagggagatg gccacccggg
      901 tcgtccgggc cgccgcaccg ctgccggtgc tggtcgtgtg cgatgacgaa gccgtgcggt
      961 cctgggcggt ggacgtcgga gccgaacccc actggacccc gggtctcggg ctcatcggcg
     1021 ccgtcgaggc cggcgtcgac gctgctgccg cggccggagc cgaccgggtc ctggtggccc
     1081 acgccgacct gccgctcgcc accgggctgg gccacgtcgt cggcgagtcc ggcgtggtga
     1141 tcgtccccga ccgctggaac gacggcacca acgtgatcgc ggtgccggcg acggccggct
     1201 tccgcttcgc ctacggcccc ggctcggcgg agcgccacca ggccgaggcc caccgcctcg
     1261 ggctggcggt ccagatcgtc gacgaccacg tgctcggctg ggacgtcgac gtgccgggtg
     1321 acctgctgct cccgagcggc gaggacctgg ccgccgggat cccgtcgtga gcggccccgc
     1381 gtcccgggac ctcccggtcc ccgagcgggc gctcgccgtg ggcgcccacc ccgacgacat
     1441 cgagttcggg gccggcgcca ccctcgcccg ctgggcggcc gccggctgcg aggtgtcgct
     1501 gctcgtgtgc accgacgggt ccaagggcac gtgggatccc gatgccgacc tgaccgccct
     1561 cgtcgccacc cgccaggagg aggctcgcgc tgccgcccag gcgctgggcg ccaccggcga
     1621 ggtcgtgttc ctgggggtca ccgacggcga actggcgaac gaccgagcca cccgctccga
     1681 gctggcccgg tggatccgca tcctgcggcc cggcgtcctc ttgggccacg acccgtggaa
     1741 gcgctaccgc ctccaccccg accaccgcgc cggcggcttc ctcgtctgcg acgcgctggt
     1801 ggccgcccgg gacccgcact tcttccccga gcacggcacc gcccaccacc gtcccgatgc
     1861 cctgctgctg ttcgaggccg acgagccgga ccacgccgag cacgccgacg aggacggcct
     1921 cgccgccaag gtcgccgccc tggagtcgca ccgctcgcag tacgagtcga cgatgttcat
     1981 caccgacgac cccgaggccg gcgccgccga gcaggcccgc ttccgcgccg aggtccgcga
     2041 ccgcctggtc gaagccggcg actgggccgg catcgccctc gccgagcgct tccgcctcct
     2101 ccccaccgac cgctgaccaa gccgccccct tccgatcccg caaccccgcc acccgccacc
     2161 cgccacccat cgaagttggt gtgcgaacgg cggctgtgcc tcgtctcgca caccaacttc
     2221 ggggacgtgc gaccgatgag gagcgggtca gacggcggct actggccggt cgggatccag
     2281 ttgccgtgga agccgagcgg gacccgcgcc gggaggtgca ccgtggccac cgggccctcg
     2341 gtcggggcgg cggcgtcgag caccaccagg tcggtggtgt cgtcggggac gtcgttgacg
     2401 agcgccaggt accagccgtc gtcctcggcg gcctcggcgg agcgggggac gaacacggcc
     2461 tcgccgacga tgcggtcgcg gccgaacgag accgtctcgc tcgccttccg gtcggcgtcg
     2521 tagcggacca ggtcggcgga cggccactgc aggtcgtcgc cgtccgtgcc gagctggacg
     2581 ccccagctga accgg
//
LOCUS       DATMFS010000471         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_342311, whole genome shotgun sequence.
ACCESSION   DATMFS010000471 DATMFS010000000
VERSION     DATMFS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342311"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1055)
                     /locus_tag="VNQ33_08865"
     CDS             complement(39..1055)
                     /locus_tag="VNQ33_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWJ98257.1"
                     /translation="MAPLLSSLMGPVDVAGLAEACSTRPGNACRLVYEQTGSVGLAEF
                     VDWLTDVPLQILWVLLIAWVANQIVKRAIRRFGDRISGMQANPGTRRFRPKEPRPPFV
                     DTGEVNLRAAARAQTITGVLRSVATGFIGVFAAIYVLGILGLSLGPLVAGAGVVGVAL
                     GFGAQSLVRDFLSGFFMLMEDHFGVGDVVDLGEASGTVEQVTLRVTRLRDQNGTVWHV
                     PNGEIRRVGNKSQQWARAVLDIAVAPSADLERAAEVIGAAAAAVWELAEAKPDVLGEP
                     EVLGIEYLGPDAATIRVQGKTRPGAQWRVSRLLRVRIAEALAEAGIDLPPSTFVRQGT
                     PPTR"
     gene            complement(1130..1696)
                     /locus_tag="VNQ33_08870"
     CDS             complement(1130..1696)
                     /locus_tag="VNQ33_08870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWJ98258.1"
                     /translation="MNRNAGVGIGVVAVAVVAAIGISFVGSGSSDGSEEPAPQAECAP
                     LGDIPEGGEGKPATVDLPADKTTGEVVTTVLEPGDGPEVTDASYVTVDYLGIACSTGE
                     QFDSSWDRGEPITIAMPSVPPTDTAFSVIPGWNQGLVGQQQGALVQLDIPSELAYGAQ
                     GNPPDIAANDPLTFVVRIIEVSDTAPAS"
     gene            complement(1693..>2548)
                     /locus_tag="VNQ33_08875"
     CDS             complement(1693..>2548)
                     /locus_tag="VNQ33_08875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWJ98259.1"
                     /translation="TDEKPDKDACEADADSYLTVDLIGATADDGTVFNDTYGEDRPLT
                     MKLGQQQLIAGLETGLTGMKVGERRTIVVPAAEAYGKDGSPEQGIGPDEDLVFVVDLV
                     SVTDSPTHCNEAAVLSKGTRDGKPTAVIMPVKAPEKVKTTVITEGDGPKATGKSYLTV
                     DYVGVSCATGQQFDSSWDRDEPITIAMADATPTDTAFSVIPGWTKGLDGQKQGSVVQI
                     EIPFEDGYGEAGSAPSIGTSDPLVFIVKIIKVSDEAPPSPTTTTVAGDATTTTAAAGD
                     ATTTTAGK"
BASE COUNT          344 a          906 c          911 g          387 t
ORIGIN      
        1 cgccgccacc tgagcggtgc cgatcagctc gggtcggctc agcgggtggg cggggtgccc
       61 tggcggacga acgtcgacgg cggcaggtcg atccccgcct cggccagggc ctcggcgatg
      121 cggacccgca gcaggcggct gacccgccac tgcgccccgg gccgggtctt gccctggacc
      181 cggatggtgg cggcatcggg accgaggtac tcgatgccca gcacctccgg ctcgcccagc
      241 acgtcgggct tggcctcggc cagctcccac acggcggccg ccgcggcgcc gatcacctcg
      301 gcggcccgct cgaggtcggc actcggggcg accgcgatgt cgagcacggc gcgcgcccac
      361 tgctgcgact tgttgccgac ccgccggatc tcgccgttgg gcacgtgcca caccgtgccg
      421 ttctggtccc ggaggcgcgt gacccgcagc gtgacctgct cgacggtccc cgacgcctcg
      481 ccgagatcca ccacgtcgcc cacaccgaag tggtcctcca tcagcatgaa gaagcccgac
      541 aggaagtccc gcaccaggct ctgggcgccg aagccgaggg cgacgcccac gacgccggca
      601 ccggccacga gcgggccgag gctgaggccg aggatgccca ggacgtagat ggcggcgaac
      661 acgccgatga acccggtggc gacgctgcgc agcaccccgg tgatggtctg ggcccgggcg
      721 gccgcccgca ggttcacctc acccgtgtcg acgaacggcg ggcggggctc tttgggccgg
      781 aaccggcgcg tgcccgggtt ggcctgcatc cccgagatcc ggtcgccgaa gcgccggatc
      841 gcccgcttca cgatctggtt ggccacccag gcgatgagca gcacccagag gatctggagg
      901 ggcacgtcgg tcagccagtc gacgaactcg gcgaggccca cgctgcccgt ctgctcgtac
      961 accaacctgc aggcgttgcc cggcctcgtg gagcacgctt cggcgaggcc cgccacatcg
     1021 accggaccca tcaaggacga cagaaggggt gccatggcgg agcaacccta ccgagggcag
     1081 cccgtctcga ccgccgatcg gtcatgggcc atcggcgggg ccgggcagct caggaggcgg
     1141 gggcggtgtc gctgacctcg atgatccgga ccacgaacgt gagcggatcg ttggcggcga
     1201 tgtcgggcgg gttgccctgc gcgccgtagg ccagctccga cgggatgtcg agctggacga
     1261 gcgcgccctg ctgctggccg acgaggccct ggttccatcc ggggatcacg ctgaacgcgg
     1321 tgtcggtcgg cggcaccgac ggcatggcga tcgtgatggg ctcgcctcgg tcccacgacg
     1381 agtcgaactg ctcacccgtg gagcaggcga tgcccaggta gtcgaccgtg acgtagctgg
     1441 catcggtgac ctcgggcccg tcgccgggct ccagcacggt ggtcacgacc tcgccggtgg
     1501 tcttgtccgc cggcaggtcc acggtggcgg gcttgccctc gccgccttcg gggatgtccc
     1561 ccagcggcgc gcactcggcc tgcggcgctg gctcctccga gccatcggac gatccgctgc
     1621 cgacgaacga gatgccgatg gcggcgacga cggcaacggc cacgacgccg atgccgaccc
     1681 cggcgttgcg gttcacttgc cggccgtggt ggtcgtggcg tcaccagcgg ccgcggtggt
     1741 ggtcgtggcg tcaccagcca cggtcgtggt ggtcggcgac ggcggagcct cgtcggacac
     1801 cttgatgatc ttcacgatga acacgagcgg atcgctggta ccgatgctgg gcgcgctgcc
     1861 ggcctcgccg tagccgtcct cgaacgggat ctcgatctgg acgaccgacc cctgcttctg
     1921 cccgtcgagg cccttggtcc aaccggggat gacgctgaac gccgtgtcgg tgggcgtggc
     1981 gtcggccatg gcgatcgtga tgggctcgtc gcggtcccac gacgagtcga actgctggcc
     2041 ggtggcgcag gacacgccca cgtagtccac ggtgaggtag ctcttgcccg tggccttggg
     2101 gccgtcgcct tcggtgatga ccgtggtctt gaccttctcc ggtgccttca ccggcatgat
     2161 caccgccgtg ggcttgccgt cgcgggtgcc cttcgagagc accgccgcct cgttgcagtg
     2221 cgtgggcgag tcggtcaccg agaccaggtc gaccacgaac accaggtcct cgtccggacc
     2281 gatcccctgc tccggcgagc cgtccttgcc gtacgcctcg gcggccggga ccacgatcgt
     2341 gcgccgctca cccaccttca tgccggtgag ccccgtctcg agcccggcga tgagctgctg
     2401 ctggccgagc ttcatggtga ggggccggtc ctcgccgtag gtgtcgttga acaccgtgcc
     2461 gtcatcggcg gtggccccga tgagatcgac ggtcaggtag ctgtcggcgt cggcctcgca
     2521 cgcgtccttg tccggcttct cgtcggtg
//
LOCUS       DATMFS010000472         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_319599, whole genome shotgun sequence.
ACCESSION   DATMFS010000472 DATMFS010000000
VERSION     DATMFS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319599"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VNQ33_08880"
     CDS             complement(<1..2022)
                     /locus_tag="VNQ33_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ98260.1"
                     /translation="MNTNHLHHRTGALAELRRLVPARGLSRHEARRVAERQAAMLLDF
                     YGIDDGPIPIAVFATLPRTSVGLDPTLPTSGMSYWTGDEWRLVARADEHPNRQRFSLA
                     HEFKHVIDHPSRDLLYPDHAAREDTADYFAACLLMPKVAIRRAWCAGIQDLDDLSERF
                     MVSTPAIQRRLVDLGLTDRMRSRRYQCARPSRALTPARAFAIAGHPSTDPKSPQVTGT
                     EYPTVNHEAALRGLVSYPPQHPKAKVRRAVLYLRVSSTSQVNTDYDPEGISIPAQRGS
                     CTRKATQIGDIEIVDEYVEPGRTGTNMAGRPAFQEMLRRIRQDRDIDVVIVYKLSRMN
                     RNRFDDAMVMAQLRQYGVTLVSATEMIDDTPEGQLLHGVLATINEFRSASDGADIRDK
                     MAEKARRGGTIGRAPIGYLNTKDTFEGREIATVAIDPERGPLVRQAFELYATGSYSLA
                     ALCDELTNRGLRTKPGRFPPGPISDSKLAKLLRDPYYRGIVTYKGDQYPGRHEPLIDQ
                     ELFDTVQAELGTRQIAGERQRVHDHYLKGTLYCAARHRRGRESRLLVQKSTGRRGGTY
                     YYFFCTARQQGNCTERHLPRADVETAVEDHYRGLALDPELTALITTQLTDTLSDLQTA
                     TREATRQTNARIAKLDRQEENLLDLIADAEIDTARAKTRLRRITAERETL"
     gene            complement(2071..2508)
                     /locus_tag="VNQ33_08885"
     CDS             complement(2071..2508)
                     /locus_tag="VNQ33_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ98261.1"
                     /translation="MPRGQNPQITEFSKYLRHRRRELQIPLSELESRTGIHNSRLSRW
                     ERGIEMPDRPERLSALARGLDVDLADLYALAGIELPTTLPTLRPYLRSKYGSTLPADA
                     LDEIARYSDYIAARHGVVTGPRPGEDEIGPPRRPEPAADEHTP"
     gene            complement(2614..3207)
                     /locus_tag="VNQ33_08890"
     CDS             complement(2614..3207)
                     /locus_tag="VNQ33_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HWJ98262.1"
                     /translation="MTGPGETPAAPEMEPRDGPRVYVASLSDYNAGVLHGAWLNADQP
                     VEDLEDGINTMLAASPTTRRYGDRAEEWAIHDHEGFGPDIALGEYESLERVSRLARGL
                     IEHGDAFGAWISIQDDQDQTSEDFTDHFAGEHASLTDWGTELLDELGFDPSELPGVPE
                     SLRPYVQVDVEAWTRDMVCGGDIDTVGSKAGVYVFWP"
     gene            3249..>3455
                     /locus_tag="VNQ33_08895"
     CDS             3249..>3455
                     /locus_tag="VNQ33_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98263.1"
                     /translation="MTFFGVLMIGVMAFGPPLCLVKPWRYEEMMELWNAPPPPPVASD
                     KAFVALGLRYGYLAWRCLVAFLLTT"
BASE COUNT          445 a          957 c         1344 g          709 t
ORIGIN      
        1 gagggtctct cgttcggcgg tgatgcgtcg gagccgggtc ttggcgcggg cggtatcgat
       61 ctcggcgtcg gcgatgaggt cgaggaggtt ctcttcttgg cggtcgagtt tggcgatgcg
      121 ggcgttggtt tggcgggtgg cttcgcgtgt cgcggtttgg aggtcgctga gggtgtcggt
      181 gagctgggtg gtgatgagcg cggtgagttc ggggtcgagg gcgaggccgc ggtagtggtc
      241 ttcgacggcg gtttcgacgt cggcgcgggg gaggtggcgt tcggtgcagt tgccttgttg
      301 tcgggcggtg cagaagaagt agtagtaggt gccgcctcgg cgtccggtgg atttctggac
      361 gaggagccgg gactcgcggc cgcgtcggtg gcgggcggcg cagtagaggg tgcctttgag
      421 gtagtggtcg tggacgcgtt ggcgttctcc ggcgatctgg cgggttccga gttcggcttg
      481 gacggtgtcg aagagttctt ggtcgatgag cggttcgtgg cgtccggggt attggtcgcc
      541 tttgtaggtg acgatccctc ggtagtaggg gtcgcgcagc agtttggcga gtttggagtc
      601 ggagatcggc ccgggtggga accggccggg tttggtgcgc aggccccggt tggtgagttc
      661 gtcgcagagc gcggcgaggg agtagctgcc ggtggcgtag agctcgaagg cttggcggac
      721 gagggggccg cgttcggggt cgatggcgac ggtggcgatc tcgcggcctt cgaaggtgtc
      781 tttggtgttg aggtagccga tgggggcgcg gccgatggtt ccgccgcggc gggctttctc
      841 ggccatcttg tctcggatgt cggcgccgtc ggatgcggag cggaattcgt tgatggtggc
      901 gaggacgccg tggaggagct gtccttcggg ggtgtcgtcg atcatctcgg tggcggacac
      961 gagggtgact ccgtattggc ggagctgggc catgaccatg gcgtcgtcga accggttgcg
     1021 gttcatgcgc gacagcttgt agacgatgac gacgtcgatg tctcggtctt ggcggatgcg
     1081 ccgcagcatc tcttggaagg cgggtctgcc ggccatgttg gtgccggtgc ggccgggttc
     1141 gacgtactcg tcgacgatct cgatgtcgcc tatctgggtg gctttgcggg tgcacgagcc
     1201 gcgctgggcg gggatggaga tcccttcggg gtcgtagtcg gtgttgacct gcgaggtcga
     1261 tgagactcgc aggtacagga ccgcccggcg caccttggct ttcggatgct gcggcgggta
     1321 tgaaacgagg ccgcgcagag cggcctcgtg gttgacggtc gggtactcgg ttccggtcac
     1381 ctgcgggctc ttcgggtcgg tgctggggtg tccagctatg gcgaacgccc gcgcgggcgt
     1441 caaggcccgt gatgggcggg cgcactggta gcgccgtgac cgcatccggt cggtgagccc
     1501 caggtcgaca aggcggcgct gtatggcggg ggtggagacc atgaaccgtt cggagaggtc
     1561 atcgaggtct tggatgcctg cgcaccaggc ccgccggatc gccaccttgg gcatgagcag
     1621 gcaggccgcg aaataatccg cggtgtcttc gcgtgcggcg tggtcggggt agaggaggtc
     1681 acgggagggg tggtcgatga cgtgcttgaa ctcgtgcgcg aggctgaagc gctgccggtt
     1741 cgggtgttcg tcggctcggg cgacgagccg ccactcgtca ccggtccaat acgacatgcc
     1801 cgaggtgggc agggtcgggt cgaggccgac gctggtgcgc ggcagggtcg cgaacaccgc
     1861 gatcggtatc gggccgtcgt cgatgccgta gaagtccagg agcatggcgg cttggcgctc
     1921 cgctactcgg cgggcttcgt ggcggctgag cccgcgggcc gggacgagcc gccgcagctc
     1981 cgcgagcgcc ccggtccgat ggtgtaggtg attcgtgttc atgctgtgcc tcctttcagc
     2041 gttggttgtg gtgccgaccg aaccgatcct ctagggcgtg tgttcgtcag cggcgggttc
     2101 aggccgccgt ggcgggccga tctcgtcctc accgggtctc ggcccggtga cgacgccgtg
     2161 tcgggcggcg atgtagtcgc tgtagcgggc gatctcatcg agcgcatcag cgggcagggt
     2221 gctgccgtac ttggatcgca gatagggccg cagggtcggc agggtggttg gtagttcgat
     2281 cccggcgagt gcgtacaggt ctgcgaggtc gacgtcgagg ccgcgggcga gcgcggagag
     2341 ccgttcgggc cggtcgggca tctcgatgcc gcgctcccag cgcgagagcc gtgagttgtg
     2401 gatgccggta cgggactcca gctcggacaa cgggatctgc agctctctcc gcctatggcg
     2461 gaggtacttg ctgaactcgg tgatctgggg gttctgacct ctcggcattc cttgtctctc
     2521 tgcaccatgc cggtgctttc gtacctcgat tgtctcatgg aacactgcgg aaaaccagag
     2581 tcgttgacca ttccacaacg ctctgacctg ggtctacggc cagaacacat acacgcctgc
     2641 cttgctgccc acggtgtcga tgtcgccgcc gcacaccatg tcgcgggtcc aagcttcgac
     2701 gtcgacttgc acgtaggggc gcagggactc cggcacgccg ggcagttctg acgggtcgaa
     2761 gcccaactcg tcgagcagct cagtgcccca gtctgtgagc gaggcgtgtt cgccggcgaa
     2821 gtgatcggtg aagtcctcgc tggtctggtc ttggtcgtct tggatggaga tccacgcccc
     2881 gaacgcatcg ccgtgctcga tcaacccccg ggcgaggcgg ctgacacgct ccaacgactc
     2941 gtactcaccc agggcgatgt cggggccgaa gccctcgtga tcgtggatgg cccactcctc
     3001 cgctcggtcg ccgtagcgcc gcgtcgtcgg tgaggcggcg agcatcgtgt tgatgccgtc
     3061 ctccaggtcc tcgacgggct ggtcggcatt cagccaggcg ccgtggagga ccccggcgtt
     3121 gtagtcgctg agggaggcga cgtagaccct tggcccgtcg cgcggctcca tctcaggagc
     3181 agccggggtt tcgccggggc cggtcatcgt cgtgctccac tgtcagaggg cctggtcatg
     3241 atgtcgccat gaccttcttc ggcgtgttga tgattggggt gatggcgttc gggccgccgc
     3301 tgtgtctcgt gaagccgtgg cgctacgagg agatgatgga gctgtggaac gccccgccgc
     3361 caccaccggt ggcctctgac aaggcgttcg tggctctggg gctgcgctac ggctatctgg
     3421 cctggcgttg tctcgtcgcg ttccttctca cgacg
//
LOCUS       DATMFS010000473         3790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_247839, whole genome shotgun sequence.
ACCESSION   DATMFS010000473 DATMFS010000000
VERSION     DATMFS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247839"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VNQ33_08900"
     CDS             complement(<1..193)
                     /locus_tag="VNQ33_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWJ98264.1"
                     /translation="MLNRPTFSLSHHSAPHRARFKSRAGEIGFTLVELVVCIAILGVV
                     IIPLSATMLVGFRSTESSES"
     gene            complement(187..819)
                     /locus_tag="VNQ33_08905"
     CDS             complement(187..819)
                     /locus_tag="VNQ33_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98265.1"
                     /translation="MRIRHAAGSTRSGSGESRRGEGGETLVEVLVMIVLMGVGFAAIF
                     SGLMTVSRINDANARRTKASDAIQSWAEGLQQPARSLTGSPTNPYTYVDCATAATYGR
                     EGQPNNLMPTSWANEPMNQAPVKVEYLSGYDANGEPQFYSDVNVCYSSMVAGKDKGLQ
                     RITLKVETPAGQKPRVVDTLVILKRRQTCPPVTNTSDGSSRFDNADLGPC"
     gene            complement(819..2033)
                     /locus_tag="VNQ33_08910"
     CDS             complement(819..2033)
                     /locus_tag="VNQ33_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWJ98266.1"
                     /translation="MAVFKYTAVTPDGQQVKDSVEGVSLTAAENDLLRQNLRVVKIKE
                     KKGFSQIEVSPERVPRQEVMHFSRQVAAFVRTGIPIIDAVRVVEDGTDSKRFKSILAD
                     VRESLEGGVPFSDALRPHSAVFPPYYLGILRSAELTGQLDTVLDQLAIYIERDMTSRS
                     KVKSALTYPAVVVVMSILTMLVMVAFVLPRFVEFFDSLDAELPLPTRMLLGFSAFMQN
                     YWWALILVAIAVVGFNMWVSRNESARVKRSEILMSLPVVGIILRYSAVERFCRIIGAM
                     MRAGVPLPDTMSAAIDSTNNPVFQQRLRAARDEMLEGEGVAGPLERTDLFPSAAIQMI
                     RVGEETGTLDQQIESAANFYATETEYKVNRLTDLFEPAVVLFMGLVVGFVAVALISAM
                     YGLLGGIEQDDK"
     gene            complement(2086..>3790)
                     /locus_tag="VNQ33_08915"
     CDS             complement(2086..>3790)
                     /locus_tag="VNQ33_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWJ98267.1"
                     /translation="PQVQQDPAPTPTGRIGEILVADGAIDEAQLASALELQKSTGDRL
                     GTVLVDTGVTARQQVTEALAKQYGLRTISLDRAVLDPQSIAVLGEATARELGVVPLSR
                     SSGRVTVAVGDPARPGVFEGVLAQATQPVTFVVVDPDRIDETIDKAYQASSTVSDAVK
                     VFEARAFERGGPATTVAEVDSSAPIVQVVNTILTQAVRDRASDVHIEPMGDRVRIRNR
                     IDGALHEVLSLPQSMSQALVSRIKIMADMNIVERRRPQDGQMEAEVDGSPLDIRISTT
                     STQYGEKTVLRILDKARALYDLSTLGMPPDTSALYADIIKSPYGMVVCAGPTGSGKTT
                     TLYASLMAINSDEINVMTVEDPVEYTFSTINQISIQEQAGVTFAAGLRSILRQDPDAI
                     LVGEIRDVETARIAIQAALTGHFVLSTIHATDATSALHRFLDMGIESFLLASSMLAVV
                     GQRLIRRNCTECLAPYEPAAEDLAFYERAGGKPKEQFIRGTGCAVCSDTGYVGRIGVY
                     EVLRVTDEMRDLVVRNAPVDEIRKLAIDQGMVPLRDQALRLVADDVTTIAEVMRTIYI
                     L"
BASE COUNT          623 a         1198 c         1333 g          636 t
ORIGIN      
        1 ccgactccga cgattcggtg gaacggaagc ccacgagcat cgtggccgac agcgggatga
       61 tgacgacgcc gaggatggcg atgcacacca cgagctcgac gagcgtgaag ccgatctcgc
      121 cggcgcgcga cttgaatcgg gcccggtggg gagcggagtg gtgtgagagc gagaacgtgg
      181 gtcggttcag catggtccta ggtccgcgtt gtcgaatcgg gagctgccgt cggaggtgtt
      241 cgtcaccggc ggacaggtct gtcgccgctt gaggatgacc agggtgtcga ccacgcgcgg
      301 cttctggccg gcgggggtct cgaccttgag cgtgatgcgc tggaggccct tgtccttgcc
      361 ggccaccatg ctcgagtagc agacgttcac gtcggagtag aactgcggtt cgccgttggc
      421 gtcgtagccc gacaggtact ccaccttcac cggtgcctgg ttcatgggct cgttcgccca
      481 cgacgtcggc atcaggttgt tcggctggcc ctcacgtccg tacgtcgccg ccgtggcgca
      541 gtccacgtag gtgtacgggt tcgtcgggct gccggtcagc gaccgcgccg gctgctggag
      601 gccttcggcc cagctctgga tggcatcgct cgccttggtg cgccgagcgt tggcatcgtt
      661 gatccgcgac accgtcatca acccgctgaa gatcgcagcg aagcccacgc ccatgaggac
      721 gatcatcacc aggacctcga cgagcgtctc gccgccttcg ccccggcggc tctccccgga
      781 cccggagcgc gtcgacccag cagcgtgacg gatcctcact acttgtcgtc ctgttcgatg
      841 ccgccgagga ggccgtacat ggcggagatc agcgccacgg cgacgaaacc gacgaccaag
      901 cccatgaaga gcacgacggc cggctcgaag aggtcggtca ggcggttgac cttgtattcg
      961 gtctccgtcg cgtagaagtt ggcggccgac tcgatctgct gatcgagcgt gcccgtctcc
     1021 tcgcccactc gaatcatctg gatcgccgcg gacgggaaca ggtcggtccg ctccagcggg
     1081 ccagcgacgc cttcgccttc cagcatctca tctcgggcgg cgcgcagccg ctgctggaag
     1141 accgggttgt tggtggagtc gatggccgcg gacatcgtgt cgggcagcgg cacgcccgct
     1201 cgcatcatcg cgccgataat gcggcagaag cgctcgacgg ccgagtagcg caagatgatg
     1261 cccaccaccg gcagcgacat caagatctcc gagcgcttca cccgtgcgga ctcgttgcgg
     1321 gacacccaca tgttgaagcc gacgacggcg atggctacca ggatcagcgc ccaccaatag
     1381 ttctgcatga aggcggagaa cccaaggagc attcgtgtcg gcagcggcag ctcggcgtcg
     1441 aggctgtcga agaactccac gaagcggggc agcacgaacg cgaccatgac gagcatggtc
     1501 aggatgctca tgacgaccac gaccgcggga taggtcaagg cgctcttgac cttcgaccgg
     1561 gaggtcatgt cccgctcgat gtagatcgcg agctggtcga gcacggtgtc gagctggccg
     1621 gtgagctcag ccgagcgcaa gatgccgagg tagtacggcg ggaacaccgc cgagtgcggg
     1681 cgcagcgcat cggagaacgg cacgccgccc tcgagcgact cgcgcacgtc ggcgaggatc
     1741 gacttgaagc gcttcgagtc ggtgccgtcc tcgaccaccc gcacggcgtc gatgatcggg
     1801 atgccggtcc gcacgaacgc cgccacctgg cgggagaagt gcatgacctc ctgtcgaggg
     1861 acgcgctcag gcgacacctc gatctgcgag aagcccttct tctccttgat cttcacgacc
     1921 cggaggttct ggcgcagaag gtcgttctcg gccgcggtga ggctcacgcc ttccaccgaa
     1981 tccttgacct gctggccatc gggtgtaacg gccgtgtact tgaacactgc catggtggga
     2041 ctccccccaa gagagctcgg tcctgcgcga aaagcgcaag aacacttaca gaatgtagat
     2101 cgttcgcatt acttcggcaa ttgtggtgac atcatctgcc acgagacgca acgcctgatc
     2161 tcgaaggggc accatgccct ggtcgatggc gagcttgcgg atctcgtcga ccggcgcgtt
     2221 gcggaccacc aggtcgcgca tctcgtcggt gacccgcagg acctcgtaga cgccgatgcg
     2281 cccgacgtaa ccggtgtcgg agcagacggc gcagcccgtg ccccggatga actgctcctt
     2341 cggcttgccg ccggcgcgct cgtagaaggc caggtcctcg gcagccggtt cgtagggcgc
     2401 caggcactcg gtgcagttgc gccggatgag gcgctgaccg acgacggcga gcatcgacga
     2461 ggccaggagg aacgactcga tgcccatgtc gaggaagcgg tggagggcgc tcgtggcgtc
     2521 ggtggcgtgg atcgtcgaca gcacgaagtg gccggtgagg gcggcctgga tggcgatccg
     2581 ggcggtctcc acgtcgcgga tctcgccgac gaggatggcg tcggggtcct ggcgcaggat
     2641 cgagcggagg ccggctgcga aggtgacccc cgcctgctct tggatgctga tctggttgat
     2701 cgtcgagaac gtgtactcga cgggatcctc gaccgtcatc acgttgatct cgtcgctgtt
     2761 gatggccatc agcgaggcgt agagcgtggt ggtcttgccc gatccggtgg gcccggcgca
     2821 cacgaccatg ccgtagggcg acttgatgat gtcggcatac agcgccgagg tgtcgggcgg
     2881 catgccgagc gtggagaggt cgtagagggc ccgagccttg tccaggatcc gcagcaccgt
     2941 cttctcgccg tactgggtgg aggtggtgct gatgcggatg tcgagcggcg acccgtcgac
     3001 ctcggcctcc atctggccgt cttgaggacg tcgccgctcc acgatgttca tgtcggccat
     3061 gatcttgatg cggctgacca gggcctggga catggactgc ggcagcgaca agacctcgtg
     3121 gagggcgccg tcgatgcggt tgcggatgcg gacccggtcg cccatgggct cgatgtggac
     3181 gtcggaggcg cggtcgcgca cggcctgggt gaggatggtg ttgacgacct ggacgatggg
     3241 ggcgctggag tcgacctcgg ccaccgtggt ggctggacct ccgcgctcga aggcgcgggc
     3301 ctcgaacacc ttgacggcat cggacaccgt ggacgaggcc tggtacgcct tgtcgatcgt
     3361 ctcgtcgatg cggtccgggt cgacgacgac gaaggtgacc ggctgggtgg cctgcgccag
     3421 cacgccctcg aagacgccag gccgggccgg atcgccgacg gcgacggtga cgcggccgct
     3481 gctccgagag agcgggacga caccgagctc acgagctgtc gcttcgccga gcacggcgat
     3541 cgactgcggg tccaggaccg cccgatcgag cgagatcgtc cgcaggccgt actgcttggc
     3601 gagcgcctcg gtgacctgct gccgagcggt caccccggtg tccacgagga ccgtgcccag
     3661 gcggtcgccg gtgctcttct ggagctccag cgccgatgcg agctgcgcct cgtcgatggc
     3721 cccgtcggcc acgaggatct cgccgatgcg gccggtgggc gtgggcgccg gatcctgctg
     3781 gacctggggt
//
LOCUS       DATMFS010000474         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_16639, whole genome shotgun sequence.
ACCESSION   DATMFS010000474 DATMFS010000000
VERSION     DATMFS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16639"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VNQ33_08920"
     CDS             complement(<1..816)
                     /locus_tag="VNQ33_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWJ98268.1"
                     /translation="MTESVTLTDNRTGESIEIPIVNGGVDAGAWKKLLGNIWFADPGF
                     TQTAATESAITELDGDAGILRYRGYPIEQLAEHSTYLEVAYLLIYGELPTEEQFAEWR
                     HEITYHTFIHENVRKRFMEGFHHDAHPMGMLVSAVAALSTFYPESKQISDPEIRNKQI
                     VRLIAKMPTLAAGAHRFSVGMPFVYPDNRLDFSSNFLSMMWKIAEPEYQADPILSRAL
                     DVLFILHADHEQNCGTTAMRVAGSSHADPYSATAAATAALYGPRHGGANEAVIK"
     gene            complement(938..1861)
                     /locus_tag="VNQ33_08925"
     CDS             complement(938..1861)
                     /locus_tag="VNQ33_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ98269.1"
                     /translation="MDLRQLSALTAVADHSSFSAAARALHTVQSNVSAHIANLERELG
                     TPLVDRATGLLTDAGELVVVRARRVQAELDALSADVASSLGEISGVVRLGVIGTTGRW
                     LVPKLLSELAAVHPKVRLVVIDATTTSLIPQLAAGSLDLAVVNLPAADPDVDTEVLFE
                     EEHVLLLTDGHPLAGRRSVHLADLAGVPLLLEPAGTGFRDDLDADAARAGVTLTPQAE
                     VDGMRLLASLAYQGYGAAILPASAVVEWSDGTWSTVALEGTTPRGVGLATPRRGRSSA
                     PTRSAIEVLHRVIADEVPNRPLLHLRGPGRS"
BASE COUNT          314 a          684 c          822 g          329 t
ORIGIN      
        1 cttgatgacg gcctcgttgg ccccgccgtg gcgcggtccg tagagcgcgg cggtggccgc
       61 cgcggtggcc gagtacgggt cggcgtgcga cgacccggcg acgcgcatcg cggtggtccc
      121 gcagttctgc tcgtggtcgg cgtggaggat gaacagcacg tcgagcgccc gagacaggat
      181 cggatcggcc tggtactcgg gctcggcgat cttccacatc atcgacaaga agttggacga
      241 gaagtcgagc cggttgtccg ggtagacgaa cggcatgccc acgctgaagc ggtgggcgcc
      301 ggcggcgagc gtcggcatct tggcgatcag ccgcacgatc tgcttgttgc ggatctcggg
      361 gtcggagatc tgcttggact ccgggtagaa ggtcgacagc gcggccacgg ccgacacgag
      421 catgcccatg gggtgggcgt cgtggtggaa gccctccatg aagcgcttgc gcacgttctc
      481 gtggatgaac gtgtggtacg tgatctcgtg gcgccactcg gcgaactgct cctcggtggg
      541 cagctcgccg tagatcagga ggtacgcgac ctccaggtag gtcgagtgct cggcgagctg
      601 ctcgatgggg tagccccggt accggaggat gccagcgtcg ccgtcgagct cggtgatggc
      661 cgattcggtg gcggcggtct gggtgaaacc ggggtcggcg aaccagatgt tgccgaggag
      721 cttcttccat gctccggcgt cgacgccccc gttgacgatg gggatctcga tcgactcgcc
      781 ggtgcggttg tcggtgaggg tcacactttc ggtcacgcgc ttcttcctgc ctctgtgggt
      841 tccaggtcag cattggccgg gtcgtctccg gtgaaccgcc agccgtgctt ccacggccga
      901 tggtaccggc cccggcgggg gagaggtgcc agcggcgtca ggagcgtccg gggcctcgaa
      961 ggtgcagcag cggccggttc gggacctcgt cggcgatgac ccggtggagc acctcgatcg
     1021 ccgaacgggt cggggcggag gagcgtccgc ggcggggcgt cgccaggccg acgccccggg
     1081 gcgtcgtgcc ctcgagcgcg acggtcgacc acgtgccgtc ggaccactcg acgacggcgc
     1141 tggcgggcag gatcgccgcc ccgtacccct ggtacgcgag cgaggccagg agccgcatgc
     1201 cgtccacctc ggcttgcggg gtgagggtga ccccggcccg ggcggcgtcg gcgtcgaggt
     1261 cgtctcggaa gccggtgccg gcgggctcca gcaggagcgg gacgccggcg aggtcggcga
     1321 ggtggacgct gcgccgcccg gccagcggat ggccgtcggt gagcagcagc acgtgctcct
     1381 cttcgaacag gacctcggtg tccacgtcgg ggtccgccgc cgggaggttg accaccgcca
     1441 ggtcgaggct cccggcggcg agctggggga tgagggaggt ggtggtggcg tcgatgacga
     1501 cgagccgcac cttggggtgc acggcggcca gctcgctgag cagcttgggc accagccagc
     1561 gccccgtcgt gccgatgacg cccaagcgca ccacgccgga gatctcgccc agcgaggagg
     1621 cgacgtcagc cgagagcgca tcgagctcgg cctgcacccg ccgggcgcgc accacgacca
     1681 gctcgccggc gtcggtcagc aggccggtgg cgcggtcgac cagcggcgtg cccagctccc
     1741 gctccaggtt ggcgatgtgg gccgacacgt tcgactgcac ggtgtggagg gcacgggcag
     1801 cggccgagaa gctgctgtgg tcggcgacgg cggtcagggc ggagagctgg cggagatcca
     1861 tcgcccatca tgatggcaca catcagaaac aactgttgga cagattgctt gctggagtta
     1921 cacacttggc atactcaaga ccgccgaggt gaacgggctc cccctcccga acctcgagcg
     1981 acggatcgag agcggttccc ctgccgctcc gatcgataga ccgaccggcc tcccctggcc
     2041 ggtcggtctc gttttggggt acggcgcggt acggcgtgga caggaaccgc tcgccgagga
     2101 cggtccgtgc acctggcttc ggacgaggtg caggcaacca ggcccgagg
//
LOCUS       DATMFS010000475         4227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_296343, whole genome shotgun sequence.
ACCESSION   DATMFS010000475 DATMFS010000000
VERSION     DATMFS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296343"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNQ33_08930"
     CDS             <1..225
                     /locus_tag="VNQ33_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWJ98270.1"
                     /translation="TLRAMLRDRVPPLADDDTSAARHRAHERSLQDALARIADGTYGR
                     CTWCGAPIPIERLEVVPTAEGCQACTATQR"
     gene            complement(306..584)
                     /locus_tag="VNQ33_08935"
     CDS             complement(306..584)
                     /locus_tag="VNQ33_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98271.1"
                     /translation="MTAPSIGSLEPVAKVLLVRLDEHLEQLVHTTAASEHHRGAIAAI
                     STALAQVRSGTYGRCLGCAGEIGADRLADEPEASTCEHCRHHPRALIG"
     gene            complement(581..871)
                     /locus_tag="VNQ33_08940"
     CDS             complement(581..871)
                     /locus_tag="VNQ33_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98272.1"
                     /translation="MDPDNGLDARQVELLRSRLLGARDELVGRTSTLRSEIEAVTSAL
                     AAVDAGVYGICLRCRRPIGFDVLSTQPAARLCPVCASGAPQTVPAPAGGRSR"
     gene            complement(1100..1267)
                     /locus_tag="VNQ33_08945"
     CDS             complement(1100..1267)
                     /locus_tag="VNQ33_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98273.1"
                     /translation="MYTVAMADVDRTVPALDPGGPVRPLDPPAVVRGWLHDRLETWWA
                     LLVGAPPVRLS"
     gene            1597..2937
                     /gene="gdhA"
                     /locus_tag="VNQ33_08950"
     CDS             1597..2937
                     /gene="gdhA"
                     /locus_tag="VNQ33_08950"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HWJ98274.1"
                     /translation="MIDEKLASAYAIVESRNPGEVEFLQSVREVLDSIGPVLAKHPEL
                     AEHKTLERICEPERQIIFRVPWADDQGEIHINRGFRVEFNSALGPYKGGLRFHETVNS
                     GIVKFLGFEQTFKNALTGMPIGGGKGGADFNPKGRSDNEIMRFCQSFMTELYRHIGEY
                     TDVPAGDIGVGTREIGYLFGQYKRITNRYESGVLTGKGTSWGGALVRTEATGYGTVYF
                     AECMLRAMGRSLDGKTCVVSGSGNVGIFAIEKIHQLGGTVAACSDSSGYVVDPNGIDL
                     EVLKEVKLGDRARICEYVERRHGATLVTDGSIWDVPCDIAFPCATQNELTRRDASALI
                     ANDVIAVVEGANMPTTPEAIRLLASAGVAFGPGKAANAGGVATSALEMQQNASRDSWT
                     FEHTEERLERIMANIHATCEETADTYASPGNYVVGANIAGFLRVSKAMVALGLI"
     gene            complement(3008..3442)
                     /locus_tag="VNQ33_08955"
     CDS             complement(3008..3442)
                     /locus_tag="VNQ33_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98275.1"
                     /translation="MPDRSTVEPSGGVGSTEALVSRAPTNLAAGSVGSSRDAATRARD
                     GEPGLGRSIAVAAAVSMAVVLAAVTGVLVATGAPVVTSLGIGAFAALWGGGGFGAMIG
                     GVLYAQRVEEPVSRPSLVGSDLDRTPALLAPWPSTTPVPGRR"
     gene            complement(3579..4034)
                     /locus_tag="VNQ33_08960"
     CDS             complement(3579..4034)
                     /locus_tag="VNQ33_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98276.1"
                     /translation="MSAAPNETRPTFDENSPKDGADRSLDVAPGSPARCHSRHPTKAT
                     TVTLRAQCHACTGWFDLARLYAADPCDADRCPNCAAHLGSAGLGHTTFRIERHLKALC
                     AVLGDLAESPGAFTVDSSAVRIQVLRAVDRLDEPPALNATTRISATTRR"
BASE COUNT          647 a         1523 c         1459 g          598 t
ORIGIN      
        1 accttgcggg ccatgctgcg cgaccgcgtc ccgccactgg ccgatgacga cacgagcgcg
       61 gcccgccacc gagcccacga gcggtcgctc caggacgcgc tcgcccgcat cgccgacggg
      121 acctacggcc gctgcacctg gtgcggcgcc cccatcccca tcgagcgcct cgaggtcgtc
      181 cccaccgcgg agggctgcca ggcgtgcacc gccacccagc ggtagggccc gcggtcaggt
      241 aggggccgac cggcgcctac gaacctgcgg tgcggggtgg ggcggaccac cccgcaccgc
      301 ggcgcctacc cgatgagcgc ccgcgggtgg tggcggcagt gctcgcacgt gctggcctcc
      361 ggctcgtcag cgaggcggtc ggcgccgatc tccccggcac acccgaggca gcgtccgtag
      421 gtgccggagc gcacttgggc cagcgccgtg ctgatcgccg cgatcgcgcc ccggtggtgc
      481 tcggacgctg cggtggtgtg cacgagctgc tcgaggtgct catcgagccg gacgagcagc
      541 accttggcga ccggctcgag cgacccgatg ctgggggcgg tcatcgggag cggcctcctg
      601 ccggagccgg aacggtctgt ggtgcgccgc ttgcgcacac cgggcacagg cgcgccgccg
      661 gctgggtcga caagacgtcg aagccgatcg gccgccggca ccgcaggcag atgccgtaca
      721 ccccggcgtc gacggcagcg agcgcgctgg tgaccgcctc gatctcggag cggagcgtcg
      781 aggtccgccc gacgagctcg tctcgtgcgc cgagcagccg gctgcggagg agctcgacct
      841 gccgggcatc gagtccgttg tctgggtcca cgtccgagct gaagcgaacc agggcttcgc
      901 cccagggatg atcgatcgtg atcacgatgt accttcccga tccaccgcgt cgccggccac
      961 atgaggtcga gcggcgcgcc caggtgattt cgtccaatgt gtgtgcaacc ttgctcctcg
     1021 gtccgcgtgc atgggccgac ggatccgaag ccgaggcgat caggctgcgt gaggcggcgg
     1081 tacggtgccg ctccgtcggt cagctcagcc ggacgggagg cgcgccgacc agcagtgccc
     1141 accaggtctc gagacggtcg tggagccagc cacgaacgac cgcaggcggg tccagcgggc
     1201 ggaccggccc gcccgggtcg agggccggga ccgtgcggtc gacgtcggcc atcgcaacgg
     1261 tgtacatgag gccaaccata gccgaccaaa acgccgccgc ttccttgccc caaaccgtgc
     1321 aattccgctg ccgacccggt cctgcattca cgcctttccg ttcggtgctg atcattcgca
     1381 cgtcacgtgg cacagcacgc tcccccgtcg cccgacgtga tccggcgggc ctggagccca
     1441 ccgatgccac ggaccgcagg gctggctcgg ctgctcgacc cgcggggcga agcgcccctt
     1501 cacgccgcac ccttcgggcg cggcgtgcgg gatgcagggt gctcggctgc ccgtccctac
     1561 gatcgatcct gtcgccaatc tgactgtgag gaccctgtga tcgacgagaa gctcgccagc
     1621 gcctacgcca tcgtcgaaag ccgcaaccct ggtgaggtcg agttcctcca atccgtccgc
     1681 gaggtgctgg actcgatcgg tcccgtcttg gccaagcacc ccgagctggc cgagcacaag
     1741 acgctggaac ggatctgcga accggagcgc cagatcatct tccgagtccc ctgggcggac
     1801 gaccagggcg agatccacat caaccggggc ttccgggtcg agttcaacag cgcgctcgga
     1861 ccctacaagg gcggcctccg gttccacgag acggtcaact ccggcatcgt caagttcctg
     1921 ggcttcgagc agacgttcaa gaacgccctc accggcatgc cgatcggcgg cggcaagggc
     1981 ggtgccgact tcaacccgaa gggccgcagc gacaacgaga tcatgcggtt ctgccagagc
     2041 ttcatgaccg agctctaccg ccacatcggc gagtacaccg acgtccccgc cggcgacatc
     2101 ggcgtcggga cgcgggagat cgggtacctg ttcggccagt acaagcggat cacgaaccgc
     2161 tacgagtccg gcgtcctcac cggcaagggc accagctggg gcggtgccct cgtccgcacc
     2221 gaggcgaccg ggtacggcac cgtctacttc gccgagtgca tgctccgggc gatgggccgt
     2281 tcgctggacg gcaagacgtg cgtcgtgtcc ggctccggca acgtgggcat cttcgccatc
     2341 gagaagatcc accagctcgg cggcaccgtg gccgcctgct ccgactcgtc cggctacgtc
     2401 gtcgatccca acggcatcga cctcgaggtg ctgaaagagg tcaagctcgg tgaccgcgcc
     2461 cgcatctgcg agtacgtcga gcggcggcac ggcgccacgc tggtgacgga cggttcgatc
     2521 tgggatgtgc cgtgcgacat cgcgttcccg tgcgccaccc agaacgagct cacccgccgc
     2581 gacgccagcg ccctcatcgc gaacgacgtc atcgccgtcg tcgagggggc gaacatgccg
     2641 acgacgccgg aggccatccg cctcctcgcc agcgccgggg tcgcgttcgg ccccggcaag
     2701 gcagccaacg ccggcggcgt cgcgacgtcg gcgctcgaga tgcagcagaa cgccagccgc
     2761 gactcctgga ccttcgagca caccgaggaa cgcctcgagc gcatcatggc gaacatccac
     2821 gccacctgcg aggagaccgc cgacacctac gcctcgcccg gcaactacgt ggtgggcgcc
     2881 aacatcgccg gcttcttgcg cgtcagcaag gcgatggtgg cgctcggact gatctgaagc
     2941 ggcgcccggc aaggctcacg ggccgctacg gcgcggccgg ccctccgccg ctcgagcccc
     3001 cggcccctca tcgccgaccg ggcaccggag tggtggacgg ccacggggca agcagcgccg
     3061 gcgtgcggtc cagatcggaa ccgaccagcg acggacggga gaccggctcc tcgacgcgct
     3121 gcgcgtagag cacaccgccg atcatcgccc cgaaccctcc gccgccccag agcgcggcga
     3181 acgccccgat ccccagcgac gtgacgaccg gcgcgccggt ggccaccagg accccggtga
     3241 ccgcggcgag cacgacggcc atgctcaccg ccgccgcgac cgcgatggac cgaccgagcc
     3301 ccggctcgcc gtctcgagcc ctcgtcgccg catcgcggct gctgccgacg gacccagccg
     3361 ccaggttggt cggtgcccgg ctcacgaggg cttcggtcga gccgacgccg ccactgggct
     3421 cgacggtgct tcggtctggc atgggaggtt ccttctggtg gtgaggtggt gaggcagtga
     3481 ggtggtgcgc ccgcgccgct cggccttccg ccggcggtgc gagcacgggt gtcggggcgc
     3541 cgggcaggag gcgacgaggt tccggccagc agggatcctc agcgccgggt ggtcgcggag
     3601 atccgtgtgg tggcgttcag agcagggggt tcatcgagcc ggtcgaccgc ccgaagcacc
     3661 tggatgcgaa ccgccgagga atcgacggtg aaggcacccg ggctctcggc aagatcaccg
     3721 agcaccgcgc agagcgcctt gaggtggcgc tcgatccgga aggtcgtgtg gccgaggccg
     3781 gccgatccca ggtgggccgc acagttcgga caccggtccg cgtcacaggg gtcggcggcg
     3841 tagagccgag cgaggtcgaa ccatccggtg caggcatggc attgcgctcg aagtgtcacg
     3901 gtcgtcgctt tcgtcgggtg ccggctgtgg caccgggccg gagaccccgg tgccacatcc
     3961 agcgatctgt cagctccgtc cttcggcgag ttctcgtcga aggttggcct cgtctcgttt
     4021 ggcgcggcgc tcatgtttca ggcggcgctt ctcgagcacg gacttgccct tctttcgggt
     4081 ctttccatct cccatggttc acctcctggg cgcgtcaaag gaacgatctg gaacggacct
     4141 ccatcgatat gccaccgacg ccttctggct ggcctgcgcg cgccgatacc gacgatgccg
     4201 gggcatcgcg aaccggccga ccgctcc
//
LOCUS       DATMFS010000476         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_111284, whole genome shotgun sequence.
ACCESSION   DATMFS010000476 DATMFS010000000
VERSION     DATMFS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111284"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNQ33_08965"
     CDS             complement(<1..254)
                     /locus_tag="VNQ33_08965"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98277.1"
                     /translation="MFADAGFDVIRMADVYPDDGQFVADDDWIGRASAEGWIALTKDV
                     AIIRDHGEALAASTLRVFALPSANLTGPEMAQRFGTNLHR"
     gene            complement(313..1074)
                     /locus_tag="VNQ33_08970"
     CDS             complement(313..1074)
                     /locus_tag="VNQ33_08970"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98278.1"
                     /translation="MPLGVRPRLYDGVRPLNGACVADDTRFTRPLYTVAQASRYVGMP
                     PSTLATWAKGYTKRFPDRPDVIQGPVITSVNGSVSPGPTIPFIGLVEATVVQAFRQTG
                     LPLQRVRRALQVLADQGELEHALASERLYTDGAQVLYDYASESGDQMLRLLTVVDSGQ
                     RVFHEVIVDYLRRITFDDTWATGLILPVTKRDVLQVRPSVASGDPIFLRGGAPLSAVV
                     SRSKAGERATSIAADYEVPVTDVQEALDVVLPAAA"
     gene            complement(1079..1558)
                     /locus_tag="VNQ33_08975"
     CDS             complement(1079..1558)
                     /locus_tag="VNQ33_08975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ98279.1"
                     /translation="MAPPTFVSCSRTYPVPADQAFEHTITWPLEELFPRRFGPIPPIT
                     GTDHDGEWGTVGQVRTIHLSDGGSMQERLVTVDPPREFGYEITGITGPMAPLAARIEG
                     TWAFAPVGSGSRITWSWTIHPKSSASSLVLPVFARIWKAYARRALDQLDDLLLSAIV"
     gene            1696..2373
                     /locus_tag="VNQ33_08980"
     CDS             1696..2373
                     /locus_tag="VNQ33_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWJ98280.1"
                     /translation="MTTLENRPNTALLIVDLQNGVLAGTHDRRRVVARISRLVDQARQ
                     DAVPVVWVQHSDEQMTIDSEEWRIVPEVAPGGADPLVRKRYGDAFEDTTLAATLEELG
                     VGRLVVAGAQTDACVRSTLHGAFVRGYDTLLVSDAHTTEDLTEWGAPPPHQVIAHTNL
                     YWGFQAAPGRVAGTVETAEVDFNEKPSMWREVASPADLAAAQDPSDPQDPSVPQDPQD
                     PQDPMPR"
BASE COUNT          429 a          879 c          853 g          341 t
ORIGIN      
        1 attcggtgca ggttggtccc gaatcgttga gccatctccg gtccagtcag gttcgccgaa
       61 ggaagcgcga agacccgaag ggtcgacgcg gcgagcgctt cgccatgatc tcggatgatg
      121 gcgacatcct tcgtgagcgc gatccagcct tcggccgacg ctcgcccgat ccagtcatcg
      181 tcggcaacga actgcccatc gtctgggtac acgtcagcca tgcggatgac gtcgaaccct
      241 gcgtcggcaa acacggcagg aacgtggtgc tttccaagcc cccggtcagc aaagaaggtc
      301 agcggctccg gatcaggccg cggccgggag gaccacatcc agcgcctctt ggacatcggt
      361 caccggcacc tcgtagtcgg ccgcgatcga cgtcgcccgc tcaccggcct tcgaccggga
      421 gacgaccgcc gagagcggtg cgcccccgcg aaggaagatc ggatccccgg acgcgaccga
      481 gggcctcacc tgcagcacgt cccgcttggt gaccggcaag atcagtccgg tggcccatgt
      541 gtcatcgaag gtgatgcgcc ggaggtagtc gacgatcacc tcgtggaaca cccgctggcc
      601 cgaatcgacg acggtcagaa gccggagcat ctgatcgccc gactcgctcg cgtagtcgta
      661 gagcacctgc gccccatcgg tgtacaggcg ttctgacgcg agggcatgct ccaactcacc
      721 ctgatcggca agcacctgca aggcacgccg gacccgctgc agagggaggc cggtctggcg
      781 gaacgcctgg acgaccgtcg cttccaccaa tccgatgaac gggatcgtcg gaccgggaga
      841 caccgacccg ttcacggagg tgatcaccgg cccctggatg acgtccggcc ggtcggggaa
      901 ccgcttcgtg tagcccttcg cccacgttgc aagcgtcgat ggcggcatcc cgacgtatcg
      961 ggacgcctgg gcgaccgtgt acaggggtcg ggtgaatcgg gtgtcgtcag ccacgcacgc
     1021 tccattcaga ggacggaccc catcgtagag acgcggtctg acgcccagag gcacttcatc
     1081 agacgatggc ggagaggagg aggtcgtcga gttggtcgag ggcgcggcgg gcgtacgcct
     1141 tccagatccg ggcgaagacg ggaagcacca gggacgacgc cgacgacttg ggatggatgg
     1201 tccacgacca ggtgatccgg ctgccgctgc ccaccggcgc gaacgcccac gtgccctcga
     1261 tgcgggccgc gaggggcgcc atcggaccgg tgatgccggt gatctcgtag ccgaactccc
     1321 tcggcggatc gacggtcacc agtcgctcct gcatcgatcc gccgtcgctc aggtggatcg
     1381 tgcgcacctg tcccaccgtg ccccactcgc catcgtgatc cgtgcccgtg atcggtggga
     1441 tcggcccgaa gcgtcggggg aacagctctt cgagcggcca cgtgatcgtg tgctcgaacg
     1501 cctggtccgc cggaaccgga taggtgcgag aacacgacac gaacgtgggt ggggccatgg
     1561 ccgagagctt cgcggccggt gccgcccccg acaaccgccc gcaccgcagc ggtgaccggt
     1621 gcgaccccgt gccgcagccc ccgccgctgc gtagcgtggg gccgcagagc gcgccgcggg
     1681 caccaggagg accacatgac cacgctcgag aaccggccga acaccgcgct gttgatcgtc
     1741 gacctgcaga acggcgtgct ggctgggacg cacgaccggc gacgggtcgt ggcccgcatc
     1801 agccgactcg tcgaccaggc ccgccaggac gccgtacccg tggtctgggt gcagcactca
     1861 gacgagcaga tgacgatcga cagcgaggag tggcgcatcg tgcccgaggt cgctccgggc
     1921 ggtgccgacc cgctggtccg caagcgctac ggcgacgcgt tcgaagacac cacgctcgcg
     1981 gccacgctcg aggagctggg tgtcggtcgg ctcgtcgtcg ccggcgccca gaccgatgcg
     2041 tgcgtccgct cgacgctcca cggtgcgttc gtccgtggct acgacaccct cctcgtcagc
     2101 gacgcccaca ccaccgagga cctgaccgag tggggcgccc cgcccccgca ccaggtcatc
     2161 gcccacacca acctgtactg gggcttccag gccgcccccg gccgcgtcgc tggcacggtg
     2221 gagacggcgg aggtcgactt caacgagaag ccgtcgatgt ggcgggaagt cgccagcccc
     2281 gctgaccttg cggccgcgca ggacccctcc gacccgcaag acccctccgt gccgcaggac
     2341 ccgcaggacc cgcaggaccc catgccccgg tgacccgctg cgctccgagc agggagcagc
     2401 gtcaggcggc accgagggcg tcggcgagct gggtgtgcac cgcgaccacc gagcgggtca
     2461 gccggaagcc gagcacgccg gcagtcacgg cgcaggcggc gg
//
LOCUS       DATMFS010000477         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_296427, whole genome shotgun sequence.
ACCESSION   DATMFS010000477 DATMFS010000000
VERSION     DATMFS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296427"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VNQ33_08985"
     CDS             complement(<1..1408)
                     /locus_tag="VNQ33_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWJ98281.1"
                     /translation="MAVLDKLLRVGEGKKVKALQGLVPDINAREPEFQKLSDAGLQAK
                     TAEFRQKLDNGADLTDILVDAFAATREAASRTIGQRHYDVQLMGGAALHYGWIAEMKT
                     GEGKTLTSTLPMYLNGLTGKGLHVITVNDYLARRDSEWMGEVLGWLGLTIGLVLPDGG
                     DADFKRQQYACDITYGTNNEFGFDYLRDNMATTAANKVQRGHVYAIVDEVDSILVDEA
                     RTPLIISGRIADAAKLYYKFASIVRGLTRDVDYDVDEEKRIVAPTEEGVAKVEAALGV
                     ENMYDAVQQNMVHQFQAALKAKELYKRDKDYILQHGEVKIVDEFTGRILEGRRWSEGL
                     HQAVEAKEGVKIKEENQTLATITLQNYFRMYEKLAGMTGTAVTEAAEFASTYNLQVVP
                     IPTHRDLLRIDQPDLIYKTEDAKFEAVVEDLAERYETGQPVLVGTISVAKSEKLSRML
                     DKRGIAHEVLNAKQHTREA"
     gene            complement(1522..2130)
                     /gene="raiA"
                     /locus_tag="VNQ33_08990"
     CDS             complement(1522..2130)
                     /gene="raiA"
                     /locus_tag="VNQ33_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWJ98282.1"
                     /translation="MPVQVTVSRRHTQVPESLRIMAEEKIGRLGRFLDGLDHAEVHFW
                     EHKNPRIADKEVCEVTIEGHGHHVRCKVQAPDGYQAIDKAYDKLEHQLHKLKTKLSRR
                     QQGRPKAHKKLDALGAVAVAVADPEAIDEVVELDGVVVEDGPRIVKAKRFAIHPMSAD
                     EAAERMDLIGHGFFFFTNVETSRSAVVYRREDGDVGLIDEAD"
     gene            complement(2171..>2654)
                     /locus_tag="VNQ33_08995"
     CDS             complement(2171..>2654)
                     /locus_tag="VNQ33_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWJ98283.1"
                     /translation="VPPGLDACRALLDYVGPARQVVAQLKYRNARSAAAWLATGVAGL
                     ARPGEVDLVTWAPTTELRRRSRGFDHAEVLARRVAAELSLPCWSVLTCAPGPPQTGRT
                     LLERRDGPEFGARRTVERASVLVVDDVVTTGATLAAAARTLRTAGAARVIGVAAAHPR
                     "
BASE COUNT          347 a          890 c          946 g          471 t
ORIGIN      
        1 cggcctcacg cgtgtgctgc ttcgcgttga ggacctcgtg ggcgatgccc cgcttgtcga
       61 gcatccgcga cagcttctcg gacttggcca ccgagatggt gccgacgagc accggctggc
      121 ccgtctcgta gcgctcggcg aggtcttcca ccacggcctc gaacttggcg tcctcggtct
      181 tgtagatcag gtcgggctgg tcgatgcgca gcaggtcgcg gtgcgtgggg atcgggacga
      241 cctgcaggtt gtacgtgctg gcgaactcgg cggcctcggt gaccgcggtg ccggtcatgc
      301 cggccagctt ctcgtacatg cggaagtagt tctggagggt gatcgtggcg agggtctggt
      361 tctcctcttt gatcttcacc ccctccttgg cctcgacggc ctggtggaga ccctccgacc
      421 agcgccggcc ctcgaggatg cggccggtga actcgtcgac gatcttcacc tcgccgtgct
      481 ggaggatgta gtccttgtcc cgcttgtaga gctccttggc cttcagcgcc gcctggaact
      541 ggtggaccat gttctgctgc accgcgtcgt acatgttctc gacgccgagg gccgcctcga
      601 ccttggcgac gccctcttcg gtcggtgcga cgatccgctt ctcctcgtcg acgtcgtagt
      661 cgacgtcgcg ggtgaggccc cggacgatgc tggcgaactt gtagtacagc ttcgcggcat
      721 cggcgatgcg gccggagatg atgagcgggg tgcgggcctc gtcgacgagg atcgagtcga
      781 cctcgtccac gatggcgtac acgtggcccc gctgcacctt gttggccgcg gtggtggcca
      841 tgttgtcgcg caggtagtcg aagccgaact cgttgttggt gccgtacgtg atgtcgcacg
      901 cgtactgctg gcgcttgaag tcggcatcgc cgccgtcggg gaggaccagg ccgatggtga
      961 ggccgagcca gcccaggacc tcacccatcc actcggagtc gcggcgggcc aggtagtcgt
     1021 tcaccgtgat gacgtggagg cccttgccgg tgagcccgtt caggtacatc ggcagggtcg
     1081 acgtgagggt cttgccctca ccggtcttca tctcggcgat ccagccgtag tgcagcgcgg
     1141 ccccgcccat gagctggacg tcgtagtggc gctggccgat ggtgcggctg gcggcttcgc
     1201 gggtggccgc gaaggcgtcg acgaggatgt cggtcaggtc ggcaccgttg tcgagcttct
     1261 ggcggaactc cgccgtcttg gcctggagcc ccgcatcgga gagcttctgg aactcgggtt
     1321 cacgggcgtt gatgtcggga acgaggcctt gaagggcttt gaccttcttg ccttcgccga
     1381 cgcggaggag cttgtcgaga acagccatga ggtccgcaag tgtacgcgcc ccgctccgca
     1441 acgcagatcg gggcgccggc ggacgccgtg acgagggcgg ccgcgccgcc gggcgggggt
     1501 cccggcggca cggtcagcag atcagtcggc ctcgtcgatg aggccgacgt cgccgtcctc
     1561 gcggcggtac acaaccgccg accgcgacgt ctcgacgttg gtgaagaaga agaacccgtg
     1621 gccgatcagg tccatgcgct cggccgcctc gtcggcggac atcgggtgga tggcgaagcg
     1681 cttcgccttc acgatgcggg gaccgtcctc gacgaccacg ccgtcgagct cgacgacctc
     1741 gtcgatcgcc tcggggtcgg ccaccgccac cgccaccgcg ccgagggcgt cgagcttctt
     1801 gtgcgccttg ggccggccct gctggcgccg ggagagcttg gtcttcagct tgtggagctg
     1861 gtgctcgagc ttgtcgtagg ccttgtcgat cgcctggtag ccgtcgggcg cctggacctt
     1921 gcagcggacg tggtgcccgt ggccctcgat cgtgacctcg cacacctcct tgtccgcgat
     1981 ccgtgggttc ttgtgctccc agaagtggac ctctgcgtgg tcgaggccgt cgaggaagcg
     2041 tccgagcctc ccgatcttct cctcggccat gatcctcaag ctctcgggca cctgcgtgtg
     2101 gcgacgactg accgtgacct gcaccggcat gacctccgta cgggggatgg tcctccggat
     2161 cgtagacccg tcatcgcggg tgcgccgcgg ccaccccgat gacccgtgct gcccctgccg
     2221 tgcgcagggt ccgagcggcc gccgcgaggg ttgctcccgt ggtcacgacg tcgtcgacga
     2281 cgagcaccga cgcccgttcg accgtgcggc gagcgccgaa ctcgggcccg tcgcgccgct
     2341 cgaggagggt gcgaccggtc tgtggcggac ccggggcgca ggtgaggacg gaccagcacg
     2401 ggagcgacag ctccgccgcc acccgccggg cgaggacctc cgcgtggtcg aagccgcggc
     2461 tgcgccggcg cagctcggtg gtgggcgccc acgtcaccag gtcgacctcg ccggggcgcg
     2521 ccaacccggc gacccccgtg gcgagccacg ccgccgcgga ccgggcgttg cggtacttga
     2581 gctgcgccac gacctggcgg gccggcccca cgtagtcgag cagcgcccgg caagcgtcca
     2641 gcccgggcgg gacc
//
LOCUS       DATMFS010000478         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_248068, whole genome shotgun sequence.
ACCESSION   DATMFS010000478 DATMFS010000000
VERSION     DATMFS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248068"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VNQ33_09000"
     CDS             <1..805
                     /locus_tag="VNQ33_09000"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HWJ98284.1"
                     /translation="TATDLARDPLHVASLLAALPAETAATTRSVVRQLGVVDSHRSPL
                     WAERSLDRQLETFDVPLADVKEAAGRLGGSVNDLFVAAAAGGAGAYHRRFGVGVGELR
                     MSMPVSTRADRSPGGNAFTPTRVLVPVAADPVARFREVHERLSVTKTERAMGLTASLA
                     GLVNLLPQPMLVRLARQQVMTVDFATSNVRAAPFDLYIAGALMTGNYPLGPIAGTAWN
                     LTTMSYRGVLNLGLHVDRAAVHAPDLLREDIEASFAELLALGSANPLGA"
     gene            complement(829..1011)
                     /locus_tag="VNQ33_09005"
     CDS             complement(829..1011)
                     /locus_tag="VNQ33_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98285.1"
                     /translation="MTETAVPEIVIQEALAVVDQALGTMQQRALVSTDEVADLLLDLR
                     LLLATADAPAPVGTGV"
     gene            1144..2589
                     /locus_tag="VNQ33_09010"
     CDS             1144..2589
                     /locus_tag="VNQ33_09010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWJ98286.1"
                     /translation="MSYEIIRRIGRGGMGVVDLARDDDGREVALKRLSLHGTPEELAK
                     ARARVRREAEVLQRLDHPGVVQLREVIDEDDDLILVMDYLSGGNLAQRVNEHGPLAPD
                     EVARLADHLLDALAAAHRQGVVHRDIKPANVLFDAGGVAHLADFGVAVHRDATPGLTA
                     TEMVVGTPGFMSPEQARGDDATAASDVFCLGATLLFAATGEGPFGIGDPKLLLLRAAA
                     GRTEKVPKTLPPDLRRRIEAMLAKDPAARPSAAAARGGTDGTHPRTIVQRAVTGRSPW
                     AIAGAVLVVLLVGAGVLAVLTDGDGNQGRLAPTTVSTTARPATTTTTCAPLPYQPCGE
                     DPAPNTDGRTCLDGFADYDGDRDTGCEAEADGLPDDSKLDGAVSANLVPADDVDTYLL
                     DVGDGFQILCDGKLQVTLTAPAGVSQRVTVLDGDEVLGTKASSDDEPATVTVGDPDCI
                     FNDATTLRVRVESVGTDRSPEDYTLEATGSF"
     gene            2599..>2820
                     /locus_tag="VNQ33_09015"
     CDS             2599..>2820
                     /locus_tag="VNQ33_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ98287.1"
                     /translation="MAHPKLGFTFASLMPLGPGLALETAQQAAGLGYRSFWTAETTGP
                     EAFSLLAAAGSAAPGLDLGTGVLALQLRTP"
BASE COUNT          386 a         1112 c          968 g          354 t
ORIGIN      
        1 caccgccacg gacctggccc gcgacccgct ccacgtcgcg tcgctgctcg ccgccctgcc
       61 ggcagagacc gccgccacca cccgctcggt ggtccgccag ctcggcgtgg tcgacagcca
      121 ccgatcgccg ctgtgggccg agcgctcgct cgaccggcag ctcgagacgt tcgacgtccc
      181 gctggccgac gtgaaggagg ccgccggtcg cctgggcgga tcggtcaacg acctcttcgt
      241 cgccgccgcg gccggtggcg ccggcgccta ccaccgccgc ttcggcgtcg gcgtcggcga
      301 gctgcgcatg tcgatgcccg tcagcacgcg cgcggaccgt tcaccgggtg gaaacgcgtt
      361 cacccccacg cgggtgctcg tcccggtggc cgcggacccg gtggcgcggt tccgcgaggt
      421 gcacgagcgg ctctcggtca ccaagacgga acgggccatg ggcctcaccg cgtcgctcgc
      481 cggactcgtg aacctgctcc cccagccgat gctcgtgcgc ctcgcccgcc agcaggtgat
      541 gaccgtggac ttcgccacct cgaacgtgcg ggcggcgccc ttcgacctct acatcgccgg
      601 cgcgctgatg accggcaact accccctcgg ccccatcgcc gggaccgcct ggaacctcac
      661 caccatgagc taccgcggcg tgctcaacct cggtctgcac gtggaccggg cggccgtcca
      721 cgccccggac ctgctgcgcg aggacatcga ggcgtcgttc gccgagctgc tcgccctggg
      781 ctcagcgaac cctctgggcg cgtagaacgc acccgctggc cgcccgggct agaccccggt
      841 gcccaccggt gcgggagcgt cggccgtggc cagcaggagc cgcaggtcca agagcaggtc
      901 cgccacctca tcggtggaga cgagcgctcg ttgctgcatg gtcccgagag cttggtcgac
      961 gacggccaac gcttcttgga tcacgatctc tggcactgca gtctcggtca cgtccgccac
     1021 cttaccgggg tgggctgaca aaccacgcgc acgttgcggc cgggcgacag gacggcgcca
     1081 gccgcccgaa acacggacac gccggccgcg gccggatcga gcggacctcc taccctggcc
     1141 gccgtgagct acgagatcat ccgccgcatc ggccgcgggg gcatgggggt ggtcgatctc
     1201 gctcgtgacg acgacgggcg cgaggtggcg ctcaagcgcc tctcgctgca cggcaccccc
     1261 gaggagctcg ccaaggcgcg cgcccgggtc cgccgtgagg ccgaggtgct ccagcggctc
     1321 gaccacccgg gtgtcgttca gctccgcgag gtgatcgacg aggacgacga cctgatcttg
     1381 gtcatggact acctctcggg cggcaacctg gcgcagcggg tgaacgagca cggcccgctc
     1441 gctcccgacg aggtcgcccg cctggccgac cacctgctcg acgccctcgc ggcggcgcac
     1501 cgccagggcg tcgtccaccg cgacatcaag ccggcgaacg tgctcttcga cgccgggggc
     1561 gtcgcccacc tggccgactt cggcgtcgcc gtccaccgcg acgccacacc ggggttgacc
     1621 gcgaccgaga tggtcgtcgg gacgccgggg ttcatgtcgc cggagcaggc ccggggcgac
     1681 gacgcgaccg cagcgtcgga tgtgttctgc ctcggagcca ccctgctctt cgccgccacc
     1741 ggcgaggggc ccttcggcat cggcgacccc aagctgctgc tcctgcgggc cgcggcagga
     1801 cgcaccgaga aggtgcccaa gaccctgccg ccggacctcc ggcgccggat cgaggcgatg
     1861 ctcgccaagg acccggccgc ccggccgtcg gcggccgcgg cccgcggcgg caccgacggc
     1921 acgcacccgc gcacgatcgt gcagcgcgcc gtcaccggcc ggagcccctg ggcgatcgcg
     1981 ggtgcggtcc tcgtggtgct gctggtcggc gccggggtcc tcgccgtgct gaccgacggc
     2041 gacgggaacc agggccgcct ggcgccgacc acggtctcca ccaccgcccg cccggccacg
     2101 accaccacca cgtgcgcgcc gctcccctac caaccctgcg gcgaggaccc ggcgcccaac
     2161 accgacggcc ggacctgcct cgacgggttc gccgactacg acggcgaccg ggacaccggc
     2221 tgcgaggcgg aggccgacgg cctccccgac gactcgaagc tcgacggcgc cgtgtccgcc
     2281 aacctggtgc ccgccgacga cgtcgacacc tacctgctcg acgtgggcga cggcttccag
     2341 atcctctgcg acggcaagct gcaggtgacc ctcacggcac cggccggggt gagccagcgg
     2401 gtgacggtcc tcgacggcga cgaggtcctc ggcaccaagg ccagcagcga cgacgagccg
     2461 gccaccgtca ccgtcggcga ccccgactgc atcttcaacg acgccaccac gctgcgggtc
     2521 cgcgtcgagt cggtcggcac cgaccggagc cccgaggact acacgctcga ggcgacgggc
     2581 agcttctagg gtcggcccat ggcccatccc aagctcggat tcacgttcgc cagcctgatg
     2641 ccgctcggcc ccggcctggc gctcgagacc gcccagcagg ccgccggcct cggctaccgg
     2701 tcgttctgga cggccgagac caccgggccg gaggcgttct cgctgctcgc ggccgcgggc
     2761 agcgcagcgc cagggctcga tctcggcacg ggtgtcctcg cgctgcagct gcgcacgccg
//
LOCUS       DATMFS010000479         4083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_177594, whole genome shotgun sequence.
ACCESSION   DATMFS010000479 DATMFS010000000
VERSION     DATMFS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177594"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VNQ33_09020"
     CDS             <1..948
                     /locus_tag="VNQ33_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98288.1"
                     /translation="GLFGDLGEVRSGPQAAMVTGHNMAYCATDTIGMAEPDVANAATI
                     LQDISSFGTMPDRTQQGLLNTVFLGRLMSNDAGFVSNAAFQDSGGGPLLDTSALFYDG
                     NSQGSVVGGAYVAVDPKVEAGVLGVAGMNYSTLLERSVDFDPFFDLMKVTYPNRVDQV
                     VGLGLIQMLWDRGETNGYAAYLNASNNLPGTPHKRILIHTALGDHQVAPITAEVEART
                     AGIPISRPIYLAGRTTDVEPGWGLETLAYPSTGSGLVLWDSGADLAPLTNLPPRTGKD
                     PHGDPRSSALAQQQKSEFLKTGGTVIDVCGAAACTAPAT"
     gene            complement(1222..1809)
                     /locus_tag="VNQ33_09025"
     CDS             complement(1222..1809)
                     /locus_tag="VNQ33_09025"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWJ98289.1"
                     /translation="MAIELPPLPYAEDALASKGLSAETIQYHYGKHHATYVNVLNGLI
                     EGTEHADSSLEEIILAADAGPLFNNSAQVWNHTFYWNCLSPNGGGTPSGELGDKINSA
                     FGSYDEFASQFTEAAKTTFGSGWAWLVEGDGGLEIQKTFNADLPLKHGVKALLTVDVW
                     EHAYYIDYRNARPDYVANFLTNLVNWDFVAENLAS"
     gene            complement(1895..3757)
                     /locus_tag="VNQ33_09030"
     CDS             complement(1895..3757)
                     /locus_tag="VNQ33_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98290.1"
                     /translation="MDPTSMYRMMTADPSVKQATLTPGLVRRVWTFAHAYRSRVFAFL
                     GVTVAIAFLGLVPPLLIKSIIDDAVPNKDDGQVTVLAGLMIAVAFAIALIGMVERYLS
                     ATIGEGLIYDLRSALFDHVQRQPIAFFTRTQTGALVSRLNNDVIGAQRAVTGTLGTVV
                     SNIVTLALTLGAMFVLSWQLTLLAVVLLPIFLLPARRVGRRLSGITRDGFDLNASMNA
                     TMTERFGVSGALLVKLFGRHDDEAASFSDRAGQVRDIGVQSAMYSRTFITALTLVGAV
                     GTAVIYLVGGKLVVSGSIQIGTLVALGIYVVQIYAPLTSLTNARVDLMTAFVSFDRVF
                     EVLDTPNPIEDADDAVALTAPVGVIDLDHVTFAYPSGLEASVASLESAAQLAAPDVGA
                     GPVLHDLTVHIDQGELVALVGPSGAGKTTVSQLIPRLYDVTTGAVRLDGIDVRDLTQA
                     SLRAAIGVVSQDPHLFHESVADNLRYARPAATDDELVAAARAARIHDVIAALPDGYAT
                     VVGERGYRLSGGEKQRLAIARMLLKDPAVVILDEATSNLDSENEAAVQDALAEALRGR
                     TSIVIAHRLSTIVDADRILVLDQGRLVQQGRHVDLVDAPGLYAELYRTLVRAEV"
     gene            complement(3826..>4083)
                     /locus_tag="VNQ33_09035"
     CDS             complement(3826..>4083)
                     /locus_tag="VNQ33_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98291.1"
                     /translation="RALVARETCPDDRRGTWAVLTAEGRATIEAVAPHHVAGVREYFL
                     DKISPEELATIAGAFAKVNDQLGPSDDCLGEPCPGTGGPAS"
BASE COUNT          614 a         1328 c         1537 g          604 t
ORIGIN      
        1 gggctgttcg gtgacctggg tgaggtccgc tcgggcccgc aggcggcgat ggtcaccggc
       61 cacaacatgg cgtactgcgc caccgacacc atcggcatgg ccgagcccga cgtcgccaac
      121 gcggccacga tcctccagga catctcgtcg ttcggcacca tgcccgaccg cacgcagcag
      181 ggcctgctga acacggtgtt cctcggccgg ctcatgtcga acgacgccgg cttcgtgagc
      241 aacgccgcct tccaagacag cggcggcgga ccgctgctcg acacgagcgc gctgttctac
      301 gacggcaaca gccagggctc ggtggtgggc ggcgcctacg tcgccgtcga cccgaaggtg
      361 gaggccggcg tgctcggcgt ggcggggatg aactactcga ccctgctcga gcgcagcgtc
      421 gacttcgacc cgttcttcga cctgatgaag gtcacctacc cgaaccgggt ggaccaggtg
      481 gtgggcctcg ggctcatcca gatgctgtgg gaccgcggcg agacgaacgg ctacgccgcc
      541 tacctcaacg cctcgaacaa cctgcccggc acgccccaca agcggatcct gatccacacc
      601 gccctgggcg accaccaggt ggcgccgatc acggccgagg tcgaggcccg caccgccggc
      661 atcccgatca gccggccgat ctacctcgct ggtcgcacca ccgacgtgga gccgggctgg
      721 ggcctcgaga cgctcgcgta cccgtcgacg ggctccggcc tcgtgctgtg ggacagcggt
      781 gccgacctcg cgccgctcac caacctcccg ccccgcaccg gcaaggaccc ccacggcgat
      841 ccccgcagct cggcgctggc ccagcagcag aagtccgagt tcttgaagac cggcggcacc
      901 gtcatcgacg tgtgcggcgc cgccgcctgc accgcccccg ccacctgacc gccgcccgac
      961 tgccgcctga ccgccgcctg accgccgccc ttcctccaac ttggaggatc gtggtcgcag
     1021 agcgccatct tcctccaagt tggagccggg tggcggaagt tggtggatcg tggtcgctgg
     1081 gcgaccaaca tcctccaggt tctcgggtgc ggcgcgtgct gccccgtgcg agttcgggac
     1141 gagacgcgag gagggccgct cccgggggag cggccctcct cggacacggg aggcgcggca
     1201 gtcggccgca gcggcacagg gtcagctggc gaggttctcc gccacgaagt cccagttcac
     1261 gaggttcgtg aggaagttgg ccacgtagtc gggccgggcg ttgcggtagt cgatgtagta
     1321 ggcgtgctcc cacacgtcga cggtgagcag ggccttcacg ccgtgcttga gcggcaggtc
     1381 ggcgttgaag gtcttctgga tctccagccc gccgtcgccc tcgacgagcc acgcccaacc
     1441 ggagccgaag gtggtcttgg cggcctcggt gaactgcgag gcgaactcgt cgtaggagcc
     1501 gaaggcggag ttgatcttgt cgccgagctc accagacggg gtgccgccgc cgttgggcga
     1561 gaggcagttc cagtagaagg tgtggttcca gacctgggcc gagttgttga acagcggacc
     1621 ggcgtcggcg gcgaggatga tctcctcgag cgacgagtcg gcgtgctcgg tgccctcgat
     1681 caggccgttc agcacgttca cgtaggtggc gtggtgcttg ccgtagtggt actggatcgt
     1741 ctccgcggag aggcccttcg acgcgagggc gtcctcggcg tagggaagcg ggggcagctc
     1801 gatggccatg ggtgtggttc ctttcgcacg ttcagcgatt cgttcggacc ctacttcccg
     1861 atctccgcga tgagaaccgc cgtggcgaag ggcgtcagac ctcggcgcgc accagggtgc
     1921 ggtagagctc ggcgtagagg ccgggggcgt ccacgaggtc gacgtggcgg ccctgctgga
     1981 cgagacggcc ctggtcgagc acgaggatgc ggtcggcgtc gacgatggtg gacagccggt
     2041 gcgcgatcac gatggacgtc cggccccgga gcgcttcggc gagggcgtct tgcacggcgg
     2101 cctcgttctc gctgtcgagg ttggacgtcg cctcgtcgag gatgacgacg gccgggtcct
     2161 tgagcagcat gcgggcgatg gcgagccgct gcttctcacc gccggacagc cggtagccgc
     2221 gctcgcccac gaccgtggcg tagccgtccg gcagggcggc gatgacgtcg tggatgcgag
     2281 cggcgcgggc ggcggccacg agctcgtcgt cggtggcggc ggggcgggcg taccgcaggt
     2341 tgtcggcgac ggactcgtgg aacaggtgcg ggtcttggct gacgacgccg atggcggcgc
     2401 gcagcgacgc ttgggtgagg tcgcgcacat cgatcccgtc gagccgcacc gccccggtgg
     2461 tcacgtcgta gaggcggggg atgagctggc tgacggtggt cttgccggcg cccgaggggc
     2521 cgaccagcgc caccagctcg ccctggtcga tgtgcacggt gaggtcgtgg agcaccggcc
     2581 cggcgccgac gtcgggtgcg gcgagctggg ccgcgctctc gagcgacgcc accgaggcct
     2641 cgaggcccga tgggtaggcg aaggtcacgt ggtccagatc gatgacgccg acgggggcgg
     2701 tgagggcgac ggcgtcgtcc gcgtcctcga tcgggttggg tgtgtcgagc acctcgaaca
     2761 cgcggtcgaa cgagacgaac gccgtcatga ggtcgacccg ggcgttggtg aggctggtga
     2821 gcggggcgta gatctgcacg acgtagatgc cgagggccac cagggtgccg atctggatcg
     2881 aaccggacac caccagcttg ccgcccacca ggtagatcac cgccgtgccg acggcgccca
     2941 cgagggtgag tgccgtgatg aaggtgcggg agtacatggc gctctgcacg ccgatgtcgc
     3001 gcacctgccc ggcccggtcg gagaagctgg ccgcctcgtc gtcgtggcgg ccgaagagct
     3061 tgaccagcag ggcgcccgac acgccgaagc gctcggtcat ggtggcgttc atcgacgcgt
     3121 tcaggtcgaa gccgtcgcgg gtgatgcccg agaggcggcg gcccacccgt ctggcgggga
     3181 gcaggaagat cgggagcagc accacggcca gcagggtgag ctgccagctc aggacgaaca
     3241 tggcgcccag ggtcaaggcg agcgtgacga tgttggacac gacggtgccg agcgtgccgg
     3301 tgaccgcccg ctgggcgccg atgacgtcgt tgttgaggcg gctcaccagg gcgcccgtct
     3361 gggtgcgggt gaagaaggcg atgggctggc gctgcacgtg gtcgaacagg gccgagcgga
     3421 ggtcgtagat cagcccctcg ccgatggtgg ccgacaggta ccgctcgacc atcccgatga
     3481 gggcgatggc gaaggccacg gcgatcatca gcccggcgag cacggtgacc tggccgtcgt
     3541 ccttgttcgg cacggcgtcg tcgatgatgg acttgatgag cagcgggggc acgaggccca
     3601 agaacgcgat ggccacggtg acgccgagga aggcgaagac ccgggatcgg taggcgtggg
     3661 cgaaggtcca cacgcgccgg acgagccccg gcgtcagggt ggcctgcttg accgacgggt
     3721 cggcggtcat catccggtac atcgaggtcg gatccatggc ctgcgcaggc tacggggacc
     3781 cgaaccgatc cccgcaggtt tgggggccgg tggctcagcg gtgggtcagg aggcgggccc
     3841 acctgtgccc gggcacggct cgcccaggca gtcgtcgctg ggaccgagct ggtcgttgac
     3901 cttggcgaag gcgccggcga tggtggccag ctcctcgggt gagatcttgt cgaggaagta
     3961 ctcgcggacc ccggccacgt ggtgcggggc cacggcctcg atggtcgctc ggccttcggc
     4021 ggtgaggacg gcccacgtgc cgcggcggtc gtcggggcac gtctcgcggg cgacgagggc
     4081 ccg
//
LOCUS       DATMFS010000480         4072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_177692, whole genome shotgun sequence.
ACCESSION   DATMFS010000480 DATMFS010000000
VERSION     DATMFS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177692"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2370
                     /gene="hrpB"
                     /locus_tag="VNQ33_09040"
     CDS             <1..2370
                     /gene="hrpB"
                     /locus_tag="VNQ33_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HWJ98292.1"
                     /translation="LLDAPWLDGQRIVVLEPRRLAARAAASRMADLLGEPLGQTVGYR
                     TRDERVGGASVRIEVVTEGILVRRLQGDPTLEGTGLVVLDEVHERNLVTDLSLALVTD
                     ARRGLRPDLRVLAMSATVDADRFARVLGDDEPAPVVRSEGGTSPVDIHYRPPSARDRD
                     DAHVSRIVGEAVAGHPDGDVLVFLPGAAAINRVADRLRAPGGLPADVDVRPLFGALPT
                     VDQDLALAASPAGRRRVVLATDIAESSLTVAGVRIVVDGGLVRVPRFDPASGMTRLQT
                     EPASLASADQRAGRAGRLGPGVAYRAWDPSDATRRPCFPVAEITVADLAGFALELAVW
                     GASAAQVPLVEAPPAGAVEQAAALLRSLGAIDDRGRPTRRGRAMAGLPVHPRLAAMVL
                     AGDEAGAGWTASVLAALLEERDIVRGRPEERPADLVARARAVVGAPTDLAVDRSAART
                     VRRRAEQLARRAGTGRGELAADEVGPLVAVAYPERIAQASGRGYRLRGGGGGQLDGAD
                     PLAGAPFLAVAVVEDGRRGPRIALAAELTAAEAAAAIGSDVTEVAVVEWDTARDDLRA
                     RTEVRSGALVLDSIDGPAPPGPGTTAALVARVQATGGASLRWTPAARTLQGRLGFLHD
                     RQPGTWPDVSDAALLATVDDWLAPLLPGATSRRDLERIDLVRALRSRLDHRALGDLDR
                     LAPTSFAAADGRTVAITYEGGTARAAARVQDLYGLTLHPTVADGTVPVVVELLSPAAR
                     PVQVTADLPGFWRGSWAEVRKEMAGRYPKHDWPTDPASAAPSRRSRRPR"
     gene            2514..3293
                     /gene="aqpZ"
                     /locus_tag="VNQ33_09045"
     CDS             2514..3293
                     /gene="aqpZ"
                     /locus_tag="VNQ33_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HWJ98293.1"
                     /translation="MTLQRRLLAEVFGTFWLVLGGCGSAIFAASIVRGDVETNLGIGF
                     IGVSFAFGLTVLTMAYAVGHISGGHFNPAVTIGALVSGRFDKKDAVPYIVAQVIGGII
                     AAGVLYVIASGKSGFDIDNTKAGAFAANGYGDLSPEGYSLAACFLAETVLTGFFLLVI
                     LGATAKKAAAGFGGLAIGLALTLIHLISIPITNTSVNPARSTSQALFAGGDYIPQLWL
                     FWVAPILGAVIGGAIWRYFLEEGADQEALVEGVEVPGNAPA"
     gene            3378..>4072
                     /locus_tag="VNQ33_09050"
     CDS             3378..>4072
                     /locus_tag="VNQ33_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWJ98294.1"
                     /translation="MSTHRIRTLLAVGAAVAAGGVVWCRRRGKAMPSAPGPVGATGRL
                     QRSVVIAGTATKLGGGWAVDRARGVFADAARREELDQARQLKTAEAVAEALGNMKGAM
                     MKLGQMASYLDQGMPEPVREALAELQQDAPPMAPELAAQVIATELGSGPDVLFAEWDP
                     VPIAAASIGQVHRAMTHDGRAVAVKVQYPGVDAAIKGDLDNAGMLFTAMTFLFPGLDP
                     EPLVEELRDRIIE"
BASE COUNT          471 a         1502 c         1532 g          567 t
ORIGIN      
        1 ctcctggacg cgccctggct cgacggccag cgcatcgtgg tgctcgagcc gcggcggttg
       61 gccgctcgag cggcagccag ccggatggcc gacctgctcg gtgaaccgct cggccagacg
      121 gtcgggtacc gcacccgcga cgagcgggtc ggaggcgcgt cggtgcgcat cgaggtggtc
      181 accgagggga tcctcgtccg ccgcctccag ggcgacccga cgctggaggg gactggcctg
      241 gtggtgctcg acgaggtgca cgagcgcaac ctcgtgaccg acctgtccct cgccttggtg
      301 accgacgccc gccgggggct gcggcccgat ctgcgggtgc tcgccatgtc ggccacggtc
      361 gacgccgacc ggttcgcccg ggtgctgggc gacgacgagc ccgcgccggt cgtgcgcagc
      421 gaaggcggga cgtcccccgt ggacatccac taccgcccac cttcggcccg agaccgcgac
      481 gacgcccacg tgtcgcggat cgtcggcgag gccgtggccg gccatcccga tggcgatgtg
      541 ctcgtgttcc tccccggcgc ggccgccatc aaccgcgtgg ccgaccggct gcgggcacct
      601 ggcgggctcc ctgccgacgt cgacgtccgc ccgctgttcg gcgcccttcc cacggtcgac
      661 caggacctcg ccctggctgc ttcgcctgcc ggccgccgcc gggtcgtgct ggccaccgac
      721 atcgcggaat ccagcctcac ggtcgccggc gtccgcatcg tcgtcgacgg cgggctggtg
      781 cgggtgccgc gcttcgatcc ggcgtcgggc atgacccggc tccagaccga gccggcgtcg
      841 ctcgcgtcag cggaccagcg cgccgggcga gcaggacggc tcgggcccgg cgtcgcctac
      901 cgggcgtggg acccgtcgga cgcgacccgt cggccgtgct tccccgtggc cgagatcacc
      961 gtggccgacc tggccggctt cgccctcgag ctggcggtgt ggggcgcgtc ggccgcgcag
     1021 gtccccctcg tcgaggcacc gccagccggt gccgtcgagc aggccgcggc gctgctgcgc
     1081 tcgctgggtg cgatcgacga ccgcggccgg ccgacgcgcc gtggccgggc catggcgggg
     1141 ctgcccgtgc acccgcgcct cgcggccatg gtcctggcgg gcgacgaggc cggggccggc
     1201 tggaccgcgt cggtgctggc cgccttgctc gaagagcgcg acatcgtccg gggtcggccg
     1261 gaggagcggc ccgccgacct cgtggcccgc gcccgcgccg tggtgggcgc gcccacggac
     1321 ctggccgtcg atcgcagcgc ggcgcggacg gttcggcgtc gggccgagca gttggccagg
     1381 cgggcgggga ccgggcgcgg ggagctggcc gccgacgagg tgggaccgct cgtggccgtc
     1441 gcctaccccg agcgcatcgc ccaggcaagc gggcgcggct accggttgcg cgggggcgga
     1501 ggcggtcagc tcgacggcgc cgacccgctg gccggcgccc cgttcctcgc cgtggccgtg
     1561 gtcgaagacg gccgccgcgg accgaggatc gccctggccg ccgagctgac cgcggccgag
     1621 gcggcggcgg cgatcggcag cgacgtgacc gaggtcgccg tggtcgagtg ggacacggcc
     1681 cgcgacgacc tgcgggcgcg gaccgaggtc cgctccggtg ccctcgtgct ggactcgatc
     1741 gacgggccgg cgccaccggg gcctggtacg acggctgccc tcgtggcacg ggtgcaggcc
     1801 accggtgggg ccagcctgcg gtggacgccg gcggcacgga cgctgcaggg gcggctcggg
     1861 ttcctccacg atcgccaacc cggcacctgg cccgacgtgt ccgatgccgc gctcctggcc
     1921 acggtggacg actggctggc gccgctgctt cccggggcca ccagccggcg ggatctcgag
     1981 cggatcgacc tggtgcgcgc cctgcggtcc cgcctcgacc accgggccct cggggatctg
     2041 gaccggctcg ctccgacgtc gttcgccgcc gccgacgggc ggaccgtcgc catcacctac
     2101 gagggcggca ccgcgcgggc ggcggcgcgc gtgcaggacc tctacggcct caccctgcac
     2161 ccgacggtgg ctgacggcac cgtgccggtg gtggtggagc tgctgtcgcc ggccgctcgc
     2221 ccggtgcagg tcaccgccga cctcccgggc ttctggcggg ggtcgtgggc cgaggtgcgc
     2281 aaggagatgg ccggccgcta cccgaagcac gactggccga ccgatccggc gtcggccgcg
     2341 ccgtctcgcc ggtctcgccg gcctcgctga ccgtcccgac gtgcacgggt gcgggggacc
     2401 atcccaggtt cgacccggcg accagtggga ggcttgctgt cagatcagcg tcacggaagc
     2461 tggctggcgg tggtctgagg tgaggtgggt tcccctgatc tggaggtagt tccatgaccc
     2521 tgcaacggcg attgctcgcc gaggtgttcg gcacgttctg gctggtgctc ggcggctgtg
     2581 gctcagcgat cttcgctgcg tccatcgtcc ggggcgatgt cgagaccaac ctcggcatcg
     2641 gcttcatcgg tgtgtcgttc gcgttcggcc tgaccgtgtt gacgatggcc tacgccgtcg
     2701 gccacatctc gggcgggcac ttcaacccgg ccgtcacgat cggggcgctc gtgtcgggcc
     2761 gcttcgacaa gaaggacgcg gtgccctaca tcgtcgctca ggtcatcggc ggcatcatcg
     2821 ccgccggcgt tctgtacgtg atcgcgtcgg gcaagagcgg cttcgacatc gacaacacca
     2881 aggccggggc cttcgcggcc aacggctacg gcgacctcag ccccgagggc tacagcctgg
     2941 ccgcctgctt cttggccgag acggtgctca ccgggttctt cctgctggtc atcctcggtg
     3001 ccaccgccaa gaaggcggcg gccggcttcg gcggcctggc catcggcttg gccctcaccc
     3061 tgatccacct gatctcgatc ccgatcacca acaccagcgt gaacccggct cgctccacca
     3121 gccaggccct cttcgccggt ggcgactaca tcccgcagct gtggctgttc tgggtggcgc
     3181 cgatcctcgg tgcggtcatc ggcggggcca tctggaggta cttcctcgag gagggcgccg
     3241 accaggaggc gctcgtcgag ggcgtcgagg tcccgggcaa cgccccggcc tgatcccggc
     3301 tcgcaccacc gacccggacc tcgtggcacg cccacggggt tcgggtcgcc ggggaccacc
     3361 ccggtagcgt cggcgccatg tcgacgcacc gcatccgcac cctgctcgcg gtgggcgcgg
     3421 cggttgccgc cgggggcgtg gtctggtgcc gtcgacgcgg gaaggcgatg ccgtcggcgc
     3481 cgggtccggt gggtgccacc ggccggctcc agcgctccgt ggtcatcgcg gggacggcga
     3541 cgaagctcgg cggcgggtgg gcggtcgacc gggcccgcgg cgtcttcgcc gacgcggccc
     3601 gccgcgagga gctggaccaa gcccgccagc tcaagacggc cgaggccgtt gcggaggccc
     3661 tcgggaacat gaagggcgcc atgatgaagc tcggccagat ggccagctac ctcgaccagg
     3721 gcatgcccga gccggtgcgg gaggcgctcg ccgagctcca gcaggacgcg ccgccgatgg
     3781 cccccgagct ggccgcccag gtcatcgcca ccgagctcgg ctcggggccc gacgtcctct
     3841 tcgccgagtg ggacccggtg cccatcgcgg cggcgtcgat cggtcaggtc caccgggcga
     3901 tgacccacga cgggcgcgcc gtcgccgtga aggtgcagta cccgggtgtc gacgcggcca
     3961 tcaagggcga cctcgacaac gccgggatgc tgttcaccgc gatgacgttc ctcttccccg
     4021 ggctcgaccc cgagccgctg gtggaggagc tgcgcgaccg catcatcgag ga
//
LOCUS       DATMFS010000481         3720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_16981, whole genome shotgun sequence.
ACCESSION   DATMFS010000481 DATMFS010000000
VERSION     DATMFS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16981"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1688
                     /gene="purF"
                     /locus_tag="VNQ33_09055"
     CDS             96..1688
                     /gene="purF"
                     /locus_tag="VNQ33_09055"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWJ98295.1"
                     /translation="MARADGTLSHDLLPGEKGPQDACGVFGVWAPGEEVAKLTYFGLY
                     ALQHRGQEAAGIAVSEGGSIVVYKDLGLVSQVFDEPTLASLRGHLAIGHCRYSTTGSS
                     TWENAQPTFRTSRSGTGVALGHNGNLVNTGELADEAGMLPGTVASDSDLVAELVAREL
                     DRNEQAREHDPALPAPTLDIALAAVLPHLQGAFSLVLMDEHRVIGVRDPNGFRPLCLG
                     RLEQGGWVLASESPALDVVGADFVREIEPGEMVVIDADGVRSARPFPADRLNPKLCVF
                     EFVYFARPDTRLYGKSVHFARVRQGELLAEQAPVEADMVMGVPESGIPAAEGYARASG
                     IPYGQGLVKNRYIGRTFIAPSQEMRALGVRMKLNPLKENLQGKRVVVVDDSVVRGTTQ
                     KQLVKMLREAGVKEVHLRLTSPPIKWSCFYGIDTGERSELLANGLEIDEIRQYLNLDS
                     IAFITLDRLISSTGTSGSGFCDACFTGNYPVAVPVTLSKGVLEADDSLGGAGCDPAPV
                     PVTTEALFGSSLLPADEARQDQ"
     gene            1745..2776
                     /gene="purM"
                     /locus_tag="VNQ33_09060"
     CDS             1745..2776
                     /gene="purM"
                     /locus_tag="VNQ33_09060"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWJ98296.1"
                     /translation="MGETYEAAGVSIEAGEEAVQRIKDKVRSTFRPEVIGDIGGFGGL
                     FAFASHRYRHPVLVSSTDGVGTKALIAQASGRFDTIGVDLVAMCVDDLVCQGAEPLFF
                     LDYIAVGKLDPDHIEQLVEGVAEGCRQAGCALIGGEMAEHPGAMDPGEFDLVGFSVGV
                     AERDQLITGEHVRAGDAVIGLPSPGIRSNGYSLARRVLLEKAQLPLDGPAFPGAHHTL
                     AEELLTPSVIYAPAVLALQRAVEVRAVCHVTGGGLAGNLVRVLPKGVDVTVDPGSWEE
                     PRIFAEIRRHGDIDQAEMRKVFNLGIGMVVVVPQDEVYRALDVLRSSGQRATEIGKIT
                     KGTGQVHFT"
     gene            2865..3134
                     /locus_tag="VNQ33_09065"
     CDS             2865..3134
                     /locus_tag="VNQ33_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98297.1"
                     /translation="MTTTRVTVTIDEAIATELRERVGRRGVSAYVEDALVWQLQRDRL
                     GEFLAQAEEESGPLSDEELEEAAALWRRDPVRPVGDERSEASPTP"
     gene            3071..3508
                     /locus_tag="VNQ33_09070"
     CDS             3071..3508
                     /locus_tag="VNQ33_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ98298.1"
                     /translation="MATRPRATGRRRALGGESHSLILDSGAVIALSRNDRRALARVAD
                     ALAAGDKVVVPAIVLAETLRGRPADAPVHRVLRKVNRFPPADEVTCRVAASLLEAAGS
                     SSTVDAVVVAVAIGLGGGVILTSDVGDLMLLADGRPEIVLARC"
BASE COUNT          535 a         1317 c         1297 g          571 t
ORIGIN      
        1 tctcgggatc gcgcggcgtg ttgttcgcgg tgggcgtgac ctctgacatg gctgcagcgt
       61 cccatggggg cctctcgctc cgtagactga tccgcgtggc gcgcgccgat ggcactctgt
      121 ctcacgatct gctccccggc gagaagggcc cccaggacgc ttgcggcgtc ttcggtgtgt
      181 gggcccccgg cgaggaagta gccaagctca cctacttcgg gctgtacgcg ttgcagcacc
      241 gcggtcagga ggcggccggc atcgccgtgt ccgagggcgg ctcgatcgtc gtgtacaagg
      301 acctcgggct ggtgtcccag gtgttcgacg agcccacgct ggcgagcctg cgcggtcacc
      361 tggcgatcgg gcactgccgg tactccacca ccggcagctc cacctgggag aacgcccagc
      421 cgactttccg caccagtcgc agcggcaccg gcgtggcgct cgggcacaac ggcaacctgg
      481 tcaacaccgg ggagctggcc gacgaagccg gcatgctgcc cggcaccgtc gccagcgaca
      541 gcgacctcgt ggccgagctg gtggcccggg agctcgaccg caacgagcaa gcccgcgagc
      601 acgatcccgc gctcccggcg cccacgctcg acatcgccct cgccgcggtc ctgccccacc
      661 tgcagggcgc cttctcgctc gtgctgatgg acgagcaccg cgtcatcggc gtccgcgacc
      721 ccaacgggtt ccggcccctg tgcctcggcc gactggagca gggcggctgg gtgctggcct
      781 cggagtcgcc cgcgctcgac gtcgtgggcg ccgacttcgt gcgcgagatc gagcccggcg
      841 agatggtcgt gatcgacgcc gacggcgtgc gctcggcccg gcccttcccg gccgaccggc
      901 tcaacccgaa gctgtgcgtc ttcgagttcg tctacttcgc ccggcccgac acccggctgt
      961 acggcaagag cgtccacttc gcccgggtcc gccagggcga gctgctcgcc gagcaggcac
     1021 cggtcgaggc cgacatggtc atgggcgtgc ccgagtccgg catccccgcg gctgagggct
     1081 acgcccgcgc cagcggcatc ccctacggcc agggcctggt caagaaccgc tacatcggcc
     1141 gcacgttcat cgccccgtcg caggagatgc gggcgctcgg cgtgcgcatg aagctcaacc
     1201 cgctgaagga gaacctccag ggcaagcggg tcgtggtcgt cgacgactcc gtcgtccggg
     1261 gcaccacgca gaagcagctg gtgaagatgc tgcgggaggc gggcgtgaag gaggtccacc
     1321 tccggctcac ctcgccgccc atcaagtggt cctgcttcta cggcatcgac acgggcgagc
     1381 gctcggagct gctggccaac ggcctggaga tcgacgagat ccgccagtac ctgaacctgg
     1441 actcgatcgc cttcatcacc ctggaccggc tcatcagctc caccggcacc agcggctcgg
     1501 gcttctgcga cgcctgcttc accggcaact acccggtggc ggtgccggtc acgctgagca
     1561 agggcgtgct cgaggccgac gactcgctcg gcggagccgg gtgcgacccg gcgcccgtgc
     1621 ccgtcaccac cgaggccctc ttcggcagca gcctgctccc ggccgacgaa gcccgccagg
     1681 accagtagat cctgctgctc gagccgatct ccgtgaccga cctgaacgag aaggaacccg
     1741 cctgatgggt gagacgtacg aagccgccgg tgtgagcatc gaggcgggtg aggaagcggt
     1801 ccagcgcatc aaggacaagg tgcgctcgac cttccggccc gaggtcatcg gtgacatcgg
     1861 cgggttcggc ggcctgttcg ccttcgccag ccaccgctac cgccacccgg tcctcgtgtc
     1921 gtccaccgac ggcgtcggca ccaaggcgct catcgcccag gcatcaggcc gcttcgacac
     1981 catcggcgtc gatctcgtgg ccatgtgcgt cgacgacctg gtctgccagg gtgccgagcc
     2041 gctgttcttc ctcgactaca tcgcggtcgg caagctcgac cccgatcaca tcgagcagct
     2101 cgtcgaaggc gtggccgagg gctgtcgcca agccggctgc gcgctgatcg gcggcgagat
     2161 ggccgagcac ccgggcgcca tggacccggg cgagttcgac ctcgtcgggt tctccgtcgg
     2221 cgtcgccgag cgcgaccagc tcatcaccgg ggagcacgtt cgcgctggcg atgccgtgat
     2281 cggcctgccg tcgcccggca tccgctccaa cggctactcg ctcgcccgcc gcgtgctgct
     2341 cgagaaggct cagctcccgc tggacggccc ggccttcccg ggcgcgcacc acacgctcgc
     2401 tgaggagctg ctgaccccgt cggtcatcta cgccccggcc gtgctcgccc tccagcgcgc
     2461 ggtcgaggtg cgggccgtgt gccacgtcac cggcggcggt ctcgccggca acctcgtgcg
     2521 cgtgctgccg aagggggtcg acgtcaccgt cgacccgggc tcctgggagg agccgcggat
     2581 cttcgccgag atccgccgcc acggcgacat cgaccaagcc gagatgcgca aggtcttcaa
     2641 cctcggcatc ggcatggtcg tcgtcgtccc ccaagacgag gtctaccggg ccctcgacgt
     2701 cctccgcagc agcggccagc gcgccaccga gatcggcaag atcaccaagg gcaccggcca
     2761 ggtccacttc acctgaccag gcccgccgct gggtcgcccc tgatgggtgg cgatccgccg
     2821 aagacccttg atgacgatcc gtcgagtcgg gtacaaccgg ggtcatgacg acgacgcggg
     2881 tcacggtgac catcgacgag gcaatcgcca ccgagctgcg cgagcgggtc gggaggcgag
     2941 gcgtctcggc ctacgtcgaa gacgcgctcg tctggcagct ccaacgggac cgactcggcg
     3001 agttcctcgc ccaggcagaa gaggagtcgg gcccattgtc cgacgaggaa ctggaggagg
     3061 ccgccgccct atggcgacga gaccccgtgc gaccggtcgg agacgagcgc tcggaggcga
     3121 gtcccactcc ctgatcctcg acagcggcgc ggtcatcgcg ctgtcccgca acgatcggcg
     3181 cgcgctcgcc cgagtcgccg atgctctggc ggcaggcgac aaggtcgtcg ttcctgcgat
     3241 cgttctggcc gagacgttgc gcggtcgccc ggcggatgcg cccgtccatc gggtgttgag
     3301 gaaggtgaac cgcttcccac ctgccgacga ggtgacgtgc cgagtggcgg cttccctgtt
     3361 ggaggcggcg ggctcgtcat cgacggtcga tgcggtcgtc gtcgcggtag cgatcggact
     3421 tggcggtggc gtgatcctca cgagcgatgt gggcgacctc atgctgcttg ccgatggtcg
     3481 cccggagatc gtccttgccc gatgttgaac ccttcgccgg caggggcgat ggagcgccgg
     3541 taggttccgg atccatgggg ttcgtgacgg tgcacgggta ctgctggccg caatcgcagg
     3601 tgggtggggg gaccgtcgct ttgcacctgt cgtcgccggg tgggcgcgag gtggcggtcg
     3661 aggtggctcg gtgcgggtgg cggcgggagg tcgtgtggtc cgacacggtg ccggccggct
//
LOCUS       DATMFS010000482         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_132996, whole genome shotgun sequence.
ACCESSION   DATMFS010000482 DATMFS010000000
VERSION     DATMFS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132996"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNQ33_09075"
     CDS             complement(<1..415)
                     /locus_tag="VNQ33_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98299.1"
                     /translation="MSDQAAPVIALRGVGRCHAGPPTVDALIDVDLEVAPGELVAVTG
                     PSGAGKSTLLQILGLLDRPTDGSYTFEGIDTRMLDGRRHAALRATRIGFVFQSFHLLP
                     HLDVVGNVELGLLYAGCARRHRRERALAVLDDLGVG"
     gene            complement(408..1727)
                     /locus_tag="VNQ33_09080"
     CDS             complement(408..1727)
                     /locus_tag="VNQ33_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWJ98300.1"
                     /translation="MSRRARGVLAVLAISLGGTCVGWVIGRSTEPPPASKAFDPGPIT
                     AAVERRTIEAKVIGRADIGATIRHEVRVGGLAGDEVVTRGVARAGSPIASGDVVAELS
                     GRPVLLLDGDVPMYRDVRPGDEGRDIVAIQRALAALGWPSDDPVGRFGPSTRAAVESW
                     YRARGYEPAGPPAEAEERSQAAEATLVTAADERDESVRALRDTADSGGDVAQARRALD
                     RAEADLRQARQAAADAAEARSTWIPRRELVLLDGLPLRISEGLATGAAPDGPIARLGG
                     GPLVAVALFPSAEVTGIEVGMPGTLDEDGSGRSWKVSVVSVEAATTADGEGEAPEGDR
                     SVIRLGFGRSPAELEGRNLRLTIVTSSSKADALVVPVGAVTTNAAGVAQVRVLRGGND
                     DDTAAVAVELGAEADGFVEVRASDHGQLEAGARVVLGRARSRAGDGE"
     gene            complement(1724..2623)
                     /locus_tag="VNQ33_09085"
     CDS             complement(1724..2623)
                     /locus_tag="VNQ33_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98301.1"
                     /translation="MKRRTVVLGVALTAVVGGAATWTIVRAVGSSDAPPASARPGLEA
                     VLDLGTGPADDLARQRRHDEVERHIEQCMRSSGFAYEPVPYVPPRSTGDIEVPADVTT
                     ARDRGYGISTALSPTESEPDRVDEPASTAPRSLGDAERAAWLERLGNPLQPGTCRALA
                     EDAADERLAGVTDDLATLTASFERAVEASPVLRRLDRRWKQCMRAAGHEVDRPADVIE
                     VIDTALTEARVATGADGVRTFDAARLRSVQELERSLAEAAVRCRVPLAHDYDEAMAAI
                     GTGLRAQHAAEVDRVRSALGRGG"
     gene            complement(2620..>3219)
                     /locus_tag="VNQ33_09090"
     CDS             complement(2620..>3219)
                     /locus_tag="VNQ33_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98302.1"
                     /translation="VLDPLDRFVLAVAARSTDHLDLEALDQADPGPTTFALALRAAAL
                     ADQVCPNPVDHVVERTIGEIEDRGVPSAANPYTASGYAALVVLAERCNRTGAAIDQVE
                     RTVRAHLHSLRRDDGLFGTPETGVDLRLTATLLVAGCRLGDPAALPRPKAVEEATSGI
                     RLDSGGAASDGSAALSLEDTLAVAQIDAAAARGCKALRP"
BASE COUNT          428 a         1158 c         1191 g          442 t
ORIGIN      
        1 cgcccactcc taggtcatcg agaacggcca gcgctcgttc ccggcggtgg cgtcgggcgc
       61 accccgcgta gagcaggccg agctcgacgt tgccgacgac gtcgaggtgg gggaggaggt
      121 ggaacgactg gaacacgaag ccgatgcggg tggcccggag cgcggcgtgc cgacgtccgt
      181 cgagcatcct ggtgtcgatc ccctcgaacg tgtagctccc gtcggtgggc cggtcgagga
      241 ggccgaggat ctgcagcagg gtggacttcc cggcgcccga tgggccggtg acggccacca
      301 gctcgcccgg cgccacctcc aggtcgacgt cgatgagagc atcgacggtc ggtgggccgg
      361 cgtggcatcg acccacgcct cgcagggcga tcaccggcgc ggcttggtca ctcaccgtcg
      421 ccagcccggc tccgggcgcg gccgaggacg acgcgggcgc cggcctccag ctgtccgtgg
      481 tctgatgcgc gcacttcgac gaacccatcg gcctccgcgc cgagctccac cgcgacggcg
      541 gcggtgtcgt cgtcattgcc tcctcggagg acgcggacct gagcgacgcc agccgcgttc
      601 gtggtcaccg caccgacggg caccaccagc gcatcggcct tcgagctcga tgtgacgatc
      661 gtgaggcgca ggttccgacc ttcgagctcg gccggcgaac gaccgaagcc gaggcggatg
      721 acggagcggt cgccttcggg tgcttcgcct tcgccatcag cggtggtcgc ggcctccacg
      781 gagacgaccg agaccttcca cgaccgaccc gagccgtcct cgtcgagagt gcccggcatg
      841 ccgacctcga tgccggtgac ctcggccgag gggaacaagg cgaccgccac cagcggaccc
      901 ccaccaagcc gggcgatggg cccatcgggg gccgcgcccg tcgcgaggcc ctccgagatg
      961 cgcagcggca gcccatcgag gaggaccagc tcgcggcggg ggatccaggt ggatcgcgcc
     1021 tcggccgcgt ccgccgctgc ctgtcgtgcc tgtcgcagat cggcctcggc acggtccagg
     1081 gctcgccggg cctgcgcgac gtcgccgccg ctgtcggcgg tgtcgcggag cgctcgaacc
     1141 gactcgtcgc gctcgtcggc cgcggtcacc agggtggctt cggccgcctg ggagcgctcc
     1201 tcggcttcgg ctggcggtcc tgctggctcg tagccgcggg cgcggtacca ggactcgacg
     1261 gcagcgcggg tggacgggcc gaagcgaccg acgggatcgt cggagggcca ccccagcgcg
     1321 gccagcgccc gttggatcgc gacgatgtca cggccctcgt cgcccggccg gacgtcccgg
     1381 tacataggga cgtccccgtc gagcagcagg accggtcgac ccgagagctc tgccaccacg
     1441 tcaccggagg cgatcgggct tccggcgcgg gcgaccccac gggtgaccac ttcatcgcca
     1501 gcgaggccgc cgactcgaac ctcgtggcgg atggtggctc cgatgtcagc acgaccgatg
     1561 accttcgcct cgatcgtgcg tcgttcgacc gcggcggtga tcggtccggg gtcgaacgcc
     1621 ttggaagccg gtggcggttc ggtggagcgg ccgatcaccc atccgacgca ggtgccgccg
     1681 agcgagatcg ccaggacagc cagcacgcct cgggcccgcc ggctcatccg cctcgtccga
     1741 gggcggatcg cacccggtcg acctctgccg cgtgctgggc gcgcaggccc gtgccgatcg
     1801 ccgccatcgc ctcgtcgtag tcgtgggcca acggcacgcg acagcggacg gcggcctcgg
     1861 cgagcgagcg ctccagctcc tgaaccgacc tcagccgtgc ggcatcgaag gtgcggacgc
     1921 cgtcggcccc tgtcgcgacg cgcgcctcgg tgagggcggt atcgatgacc tcgatgacat
     1981 ccgccgggcg gtcgacctcg tgtcctgccg ctcgcatgca ctgcttccag cggcggtcga
     2041 gccggcgcag caccgggctg gcctcgaccg cgcgctcgaa tgatgccgtc agcgtcgcca
     2101 gatcgtcagt gacgcctgcc agccgttcat cagcggcgtc ctcggcgagc gcgcggcacg
     2161 tgcctggttg gagcgggttg ccgaggcgct ccaaccacgc cgcgcgctcg gcgtctccga
     2221 gcgaccgtgg cgcggtgctc gcgggttcgt ccacgcggtc cggctccgat tcggtcgggg
     2281 agagggcggt ggagatcccg tagccgcggt ctcgggcggt ggtgacgtcg gcgggcacct
     2341 cgatgtcacc ggtcgatcga ggcggcacgt agggcacggg ctcgtaggcg aacccgctgc
     2401 tcctcatgca ctgttcgatg tgccgctcga cctcgtcgtg acgccgctgc cgtgcgaggt
     2461 catcggccgg ccccgtcccg agatcgagca ccgcctcgag cccaggccgt gccgaggcgg
     2521 gcggagcgtc cgatgatccg accgcccgca cgatcgtcca ggttgccgcc ccgccgacga
     2581 cggcggtgag ggcgaccccg aggaccacgg tccggcgctt cacgggcgca gcgccttgca
     2641 cccgcgggcc gcggcggcat cgatctgcgc caccgccagg gtgtcctcga gcgacagggc
     2701 ggcgctgccg tccgatgccg ctccaccgct gtcgaggcgg atgccgctgg tcgcctcctc
     2761 cacggccttc ggacgaggca gggcggccgg atcaccgagg cggcaaccag ccacgagcaa
     2821 ggtggcggtg agacggaggt ccacaccggt ctcgggggtg ccgaagagcc cgtcgtcacg
     2881 ccgcagcgag tggaggtggg cgcgcacggt ccgctccacc tggtcgatcg cggcacccgt
     2941 tcggttgcat cgctcggcga gcaccacaag ggcggcgtag ccgctggcgg tgtaggggtt
     3001 ggctgcgctg gggacgccgc gatcttcgat ctcgccgatg gtccgctcga ccacgtggtc
     3061 caccgggttt ggacacacct ggtcggcgag ggccgccgcc cgcagcgcga gcgcgaacgt
     3121 cgtcggtccc gggtcggcct ggtcgagtgc ctcgagatcg aggtggtcgg tggaccgggc
     3181 cgccacggcg agcacgaagc ggtcgagcgg gtcgaggac
//
LOCUS       DATMFS010000483         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_320294, whole genome shotgun sequence.
ACCESSION   DATMFS010000483 DATMFS010000000
VERSION     DATMFS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320294"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VNQ33_09095"
     CDS             complement(<1..245)
                     /locus_tag="VNQ33_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWJ98303.1"
                     /translation="MAVDQVLLAAPRGFCAGVEMAIKALAWMVRAFDAPVYCYHEIVH
                     NQSVVKRFEDLGVIFVDDVSEVPEGRPLMLSAHGSAP"
     gene            322..1761
                     /locus_tag="VNQ33_09100"
     CDS             322..1761
                     /locus_tag="VNQ33_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HWJ98304.1"
                     /translation="MSAPRVVAVAPGSPAALGGVQVGDEVLAVDGERPRDIIRWTWLT
                     DEADPVLDIARGGLELSIEVEKDAGAPLGIDVHSALFDQLRTCDNHCEFCFIYQLPPG
                     MRKSLYLKDDDYRLSFLYGNFTTLTRFTEADLERVVTEGLSPLNVSIHAVDPAARNDL
                     LRNRRGGPSLRWLRALLDHGVEVHGQIVCCPGLNDGSVLDDTLAGVLEQFPELASLCV
                     VPLGISKFNTEPRMRPTTQAEAAAVVDCVEDWQDVYLQVLGRRLVQASDEYYLLARRP
                     FPDPEVYEGFAMHEDGVGMARTFEQEFLGHADTTTAPQAGFFAWADATTSAPAASGGR
                     YEAAGDYGAPAEGYRAVRTAEAASPAGGGVAVALRPRRGAPTAVLTSEAGAAVIGPLV
                     ASLDRSDVRVVPVENRFFGGTTSVTGLLVGEDLHRVLAGQPETHRYLLPDVCLSGGRF
                     LDGGTPADLPRPVEIIPTDGAALRRALEA"
     gene            1763..>2361
                     /locus_tag="VNQ33_09105"
     CDS             1763..>2361
                     /locus_tag="VNQ33_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWJ98305.1"
                     /translation="MSDATPKPLPMVAIVGRPNVGKSTLMNRIIGRREAIVEELPGVT
                     RDRKEVEADWIGRRFRLVDTGGWMPGGSSLDEKVSRQSEQAIKEADALILVTDVSMGI
                     TEEDARIAELLQTASQPVFVVANKVDDPSREALMWELLGLGLGNPWPVSALHGLGTGD
                     MLDALVAALPEVEDDEDGEPAEPDGDRVFAVALVGRPNVG"
BASE COUNT          343 a          900 c          801 g          317 t
ORIGIN      
        1 tcgggcgccg acccgtgcgc cgagagcatc agcggccggc cctcggggac ctccgacacg
       61 tcgtcgacga agatcacgcc caggtcctcg aaccgcttca ccaccgactg gttgtggacg
      121 atctcgtggt agcagtacac cggcgcatcg aaggcccgca ccatccaggc cagcgccttg
      181 atggccatct ccacaccggc gcagaagcct cggggagcgg cgagcagcac ctggtcgacg
      241 gccatgggcc caccctaccg gccgtcctcc gaagccccag atcggagggg ggacgggacg
      301 gtcccagtag gatcgccccc tatgtctgcg ccccgagtcg ttgctgtcgc gcccggttcg
      361 ccggccgcgc tcgggggcgt gcaggtcggc gacgaggtgc tcgccgtcga cggcgagcgg
      421 ccgcgggaca tcatccggtg gacctggctc accgacgaag ccgacccggt gctcgacatc
      481 gcgcgcggcg ggctcgagct gtcgatcgag gtggagaagg atgccggcgc cccgctcggc
      541 atcgacgtgc actcggcgct gttcgaccag ctccgcacgt gcgacaacca ctgcgagttc
      601 tgcttcatct accaactgcc gccgggcatg cgcaagagcc tgtacctgaa ggacgacgac
      661 taccgcctga gcttcctgta cgggaacttc accaccctca cccgcttcac cgaggccgac
      721 ctcgagcggg tggtcaccga gggcctgtct ccgctcaacg tgagcatcca cgccgtcgat
      781 ccggccgctc gcaacgacct cctccggaac cggcgcgggg gcccgagcct gcggtggctc
      841 cgagcgctgc tcgaccacgg cgtcgaggtg cacggccaga tcgtgtgctg tcccggcctc
      901 aacgacggct cggtcctcga cgacacgctc gccggggtcc tcgagcagtt cccggagctg
      961 gccagcctct gcgtggtccc gctcggcatc tcgaagttca acaccgagcc ccggatgcgg
     1021 cccaccaccc aggccgaagc agcggcggtg gtcgactgcg tcgaggactg gcaggacgtc
     1081 tacctccagg tcctcgggcg gcgcctcgtg caggcgtccg acgagtacta cctgctggcc
     1141 cggcgcccgt tcccggatcc cgaggtctac gagggcttcg ccatgcacga ggacggcgtg
     1201 ggcatggccc gcaccttcga gcaggagttc ctcgggcacg ccgacacgac gacggccccg
     1261 caggccgggt tcttcgcctg ggccgacgcc accacgagcg cgcccgccgc ctcgggcggc
     1321 cggtacgaag ccgccgggga ctacggcgcc cccgccgagg gctaccgcgc cgtgcgcacc
     1381 gccgaggcgg catcgcccgc agggggcggc gtggccgtcg ccctccggcc ccgccgcggg
     1441 gcgcccaccg ccgtgctcac gagcgaggcg ggggccgccg tcatcgggcc gctcgtcgcg
     1501 tcgctcgacc ggtccgacgt gcgcgtggtc cccgtcgaga accgcttctt cggcggcacc
     1561 acgtcggtca ccggcctgct ggtgggggag gacctccacc gggtcctcgc cggccagccc
     1621 gagacccacc gctacctgct ccccgacgtc tgcctctccg gcggccgctt cctcgacgga
     1681 ggaaccccgg ccgacctgcc gcgccccgtc gagatcatcc ctaccgacgg cgctgcgctg
     1741 cgccgggcct tggaggcctg aagtgtctga cgccacgccc aaaccactgc ccatggtggc
     1801 catcgtcggc cgccccaacg tgggcaagtc cacgctgatg aaccggatca tcggccgccg
     1861 cgaagccatc gtcgaggagc tccccggcgt cacccgcgac cgcaaggagg tcgaagccga
     1921 ctggatcggc cgccgcttcc gcctggtcga caccggcggc tggatgcccg gtggctcctc
     1981 gctcgacgag aaggtgagcc gccagagcga gcaggccatc aaggaggccg acgccctgat
     2041 cctcgtgacc gacgtgtcca tggggatcac cgaggaggac gcccgcatcg ccgagctgct
     2101 ccagaccgcg agccagcccg tattcgtcgt ggccaacaag gtcgacgacc cgtcgcgcga
     2161 agcgctgatg tgggagctgc tcggcctcgg cctcggcaac ccctggcccg tcagcgccct
     2221 ccacggtctc ggcaccgggg acatgctcga cgcgctggtc gcggcgctcc ccgaggtcga
     2281 agacgacgag gacggcgaac cggcagagcc cgacggcgat cgggtgttcg cggtggcgct
     2341 cgtcggccgg cccaacgtgg g
//
LOCUS       DATMFS010000484         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17065, whole genome shotgun sequence.
ACCESSION   DATMFS010000484 DATMFS010000000
VERSION     DATMFS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17065"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VNQ33_09110"
     CDS             complement(<1..484)
                     /locus_tag="VNQ33_09110"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWJ98306.1"
                     /translation="MTTTFAAITAPSALELRERGRPDEIAPGMVEVDVTLCGICATDT
                     HNYTSGGQIPPATFGHEWTGTVRRVGVGVSTVEPGTRVVASVGPACGTCAQCTAGHPQ
                     HCDTAFAEANGVTPDSPDHGGFATSLVVSERRVMPVLEGLSDVEAALVEPTAVTFHAV
                     K"
     gene            complement(628..930)
                     /locus_tag="VNQ33_09115"
     CDS             complement(628..930)
                     /locus_tag="VNQ33_09115"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HWJ98307.1"
                     /translation="MTASKPAKSSTSAKILYQPVGLISSILAGLIASAVFKQVWKRAS
                     PNSEGDPPNPTQSEFPLKEILLAAVIQGAIFSGVRAIVQRQGARAFAKATGEWPGD"
     gene            complement(930..1220)
                     /locus_tag="VNQ33_09120"
     CDS             complement(930..1220)
                     /locus_tag="VNQ33_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWJ98308.1"
                     /translation="MVNIKRLPSPIIESYEWQWEGACMGVDSSVFFSPEAERGMKRHR
                     REENAKAVCATCPVIDRCREHALAVQEPYGVWGGLTESERTEILAGRQVAVG"
     gene            1348..2229
                     /locus_tag="VNQ33_09125"
     CDS             1348..2229
                     /locus_tag="VNQ33_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HWJ98309.1"
                     /translation="MHRRWTVGTLSEMLGIAAPTLRTWERRYGVGPTFRTAGGHRRYT
                     IVDADRVATMARFIESGVPAHDAAERVKRLSPHELAAIVGAGPAGLRASAHEQAVFDI
                     IEGAKELDAARVQEAAEKAIDRFGIEEAWDAAIAPAMIEVGLHWEDGRIGVAAEHLVT
                     ACVLSALRAAAHQYPTKGPARVVLASVEDEQHKLPVVALGAALARHGHAWTELGARLP
                     MESLEQFVESSEPDVVFLWSSVIVPGEEELERFAALGKRTHLFLGGTGWPHGVATSHS
                     LTETVQQLRRALGDALP"
     gene            2235..>2815
                     /locus_tag="VNQ33_09130"
     CDS             2235..>2815
                     /locus_tag="VNQ33_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ98310.1"
                     /translation="MSEQHVVTEIEGPIARVWLNRPDKLNGITFGVLDGLLEAADRIE
                     ADPNVRAVILEGRGPSFCAGLDFGQVMTDKKRVAKYFLPNPLKGTNKFQQPLWAWREL
                     SVPVIAVTRGHVFGGGIQLALAADFRFTTPDCQWSILEAKWGLVPDMSGTVALAELVN
                     ADLAKRLVMTGEVFSGEQAVEYGIASGVADDPT"
BASE COUNT          425 a          997 c          976 g          417 t
ORIGIN      
        1 gcttgaccgc atggaacgtc acggccgtcg gctcgacgag ggcggcctcg acgtcgctga
       61 gaccctcgag caccggcatg acccgacgct ccgagacgac gagcgaggtg gcgaacccgc
      121 cgtggtcggg agagtcgggc gtgacgccgt tcgcctcggc gaaggcggtg tcacagtgct
      181 gggggtggcc ggccgtgcac tgcgcacagg tgccgcacgc gggcccgacc gaggcgacga
      241 cccgggttcc gggctccacg gtcgacacgc cgacgccgac tcggcgcacc gtgccggtcc
      301 actcgtgacc gaacgtcgcg ggcgggatct ggccgcccga cgtgtagttg tgggtgtcgg
      361 tcgcgcagat gccgcacagc gtcacgtcga cctccaccat gccgggggcg atctcgtccg
      421 gccggcctcg ctcccgcagc tcgagggcgg acggggcggt gatggcggcg aacgtagtag
      481 tcacatcttc aactgttcca cggctggcga aaccggcgcg gtgcaccgtc tccggagacg
      541 acgatggccg cggggtggag tcccgcggcc atcatcggtg tgcgccgacc ggcaggagga
      601 ggggccggtc gtcgcggagt cgtgcgttca gtcgccgggc cactcgcccg tcgccttggc
      661 gaacgcgcgg gcgccctgtc gctggacgat cgcgcggacg ccggagaaga tcgcgccctg
      721 gatgacagcg gcgagcagga tctccttgag cgggaactcc gactgcgtcg ggttgggcgg
      781 atcgccctcg ctgttcgggc tggcccgctt ccagacctgc ttgaacaccg ccgacgcgat
      841 gagtcccgcg aggatcgacg agatgagccc gaccggctgg tacaggatct tcgccgacgt
      901 gctggacttc gcgggcttgg aggcggtcat cagccgacgg ccacctgacg acccgcgagg
      961 atctcggtgc gctcggactc ggtgagacca ccccagacgc cgtacggctc ctggacggcg
     1021 agcgcgtgct cacggcaccg gtcgatgacc gggcacgtgg cgcagaccgc cttggcgttc
     1081 tcctcacggc ggtgacgctt catcccgcgc tcggcttcgg gcgagaagaa gacggaggag
     1141 tcgacgccca tgcaggcgcc ctcccactgc cattcgtagg actcgatgat gggcgacgga
     1201 agtcgcttga tgttgaccat gaaacctgcc ccttccccaa gggataggac tgacacgagc
     1261 tggtgtgcac aacccggtga gtagtccgac cgtactactc aacctattca ctaggcaagc
     1321 gacttgaata ggttttatga tttccccatg caccgccgct ggaccgtggg gactctctcc
     1381 gagatgttgg gcatcgccgc ccccactctt cgcacgtggg agcgccgtta cggcgtgggt
     1441 cccaccttcc gcaccgccgg cggccaccgc cgctacacga tcgtcgacgc ggaccgtgtc
     1501 gccacgatgg cacgcttcat cgaatcgggc gttcccgccc acgacgcggc cgagcgcgtc
     1561 aagcgtctgt cgccccacga gctcgccgcg atcgtgggcg ccggaccggc gggactgcgg
     1621 gcctcggccc atgaacaggc ggtcttcgac atcatcgagg gcgccaagga gctggacgcc
     1681 gcccgcgtcc aggaggccgc cgagaaggcg atcgaccgct tcggcatcga ggaggcctgg
     1741 gacgcggcca tcgcgccggc gatgatcgag gtcggcctgc actgggagga cggccggatc
     1801 ggcgtggccg ccgagcacct cgtcaccgcg tgcgtcctca gcgccctgcg cgccgccgca
     1861 caccagtacc cgacaaaggg cccggcgcgc gtcgtgctgg ccagcgtcga ggacgagcag
     1921 cacaagctgc ccgtcgtcgc gctcggcgcg gccctcgccc gccacggcca cgcctggacc
     1981 gagctcggcg cgcgactgcc catggagtcg ctggagcagt tcgtcgagag cagcgagccc
     2041 gacgtcgtct tcctctggtc gtcggtgatc gtcccgggcg aggaggagct ggagcggttc
     2101 gccgccctgg gcaagcgcac gcacctcttc ctcggcggta cgggctggcc gcacggcgtc
     2161 gccacgtcgc actcgctgac cgagacggtg cagcagttgc ggcgcgcgct cggcgacgcc
     2221 ctaccctgat ccccatgagc gaacagcacg tcgtcaccga gatcgagggg cccatcgccc
     2281 gcgtctggct caaccgcccg gacaagctga acggcatcac gttcggggtg ctcgacggcc
     2341 tcctcgaggc ggccgaccgc atcgaggccg acccgaacgt gcgcgccgtg atcctcgagg
     2401 gccgcggtcc gtccttctgc gccggcctgg acttcggtca ggtcatgacc gacaagaagc
     2461 gcgtcgccaa gtacttcctg cccaacccgc tcaagggcac gaacaagttc cagcagccgc
     2521 tgtgggcctg gcgtgagctc tcggtgccgg tcatcgcggt cacccgcggt cacgtgttcg
     2581 gcggcggcat ccagctggcg ctcgccgccg acttccgctt caccacgccg gactgccagt
     2641 ggtcgatcct cgaggccaag tggggcctgg tccccgacat gagcggcacc gtcgccctgg
     2701 ccgagctggt caacgccgac ctcgccaagc gcctcgtgat gaccggtgag gtcttctccg
     2761 gcgagcaggc cgtcgagtac ggcatcgcgt ccggcgtcgc cgacgacccg accaa
//
LOCUS       DATMFS010000485         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_248342, whole genome shotgun sequence.
ACCESSION   DATMFS010000485 DATMFS010000000
VERSION     DATMFS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248342"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VNQ33_09135"
     CDS             <1..760
                     /locus_tag="VNQ33_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ98311.1"
                     /translation="VTAYVNALKAGDRAPSSVKRMLVSVRGLHRFLAEETEGVADPTQ
                     DVEVPKPPKGLPKALSEAEVERLLGSVTGTDAVARRDRAILEVLYGTGARIGELSGVS
                     LSDLDLDAGLVRLFGKGAKERIVPLGQFAQRALVDWLGDHGRPLLEPEQWRSRDDADA
                     VFLNRRGGRLQRGGAWLVVRQAGDAVGLGSRLTPHVLRHSCATHMLDHGADIRTVQEL
                     LGHASISTTQVYTLVSGERLQAVYQAAHPRASAG"
     gene            770..1093
                     /locus_tag="VNQ33_09140"
     CDS             770..1093
                     /locus_tag="VNQ33_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWJ98312.1"
                     /translation="MAQPDLTAVRDQLVTEQADLERQLQELSGDGDGLDADENFADSA
                     QVAAEHVENRTLAAQLQDQLDDVAAALARLDDGRYGTCEVCEQPVTAARLEAMPSTRF
                     CIDHA"
     gene            1093..1611
                     /locus_tag="VNQ33_09145"
     CDS             1093..1611
                     /locus_tag="VNQ33_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWJ98313.1"
                     /translation="MAGPAHLVKRFFGSLVPLGEPKNDRAWVHEHLLPEEQEIWRRMS
                     RQDRRHAAGVARRVERALGHEATRPVIAAALLHDCGKCVSHLGTTGRVIATLSAKVAG
                     HEMAPAWSETTGFTRRVGLYLEHPRLGADLLGMAGSAPLTVAWAAEHHLPPEDWTVPA
                     NIGQVLKDADDD"
     gene            1840..>2913
                     /locus_tag="VNQ33_09150"
     CDS             1840..>2913
                     /locus_tag="VNQ33_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98314.1"
                     /translation="MLVLLLVASVLPVAVVVLGGGVPAGAVDDFGVLSTLAGSGAVGQ
                     ADGAAGSASFDSLVDLAATYDGRYLYVLEVSSGGSRENMIRKVDTGDGSVSTFVTESE
                     APALQAPKSIQVDRFGQVWVSVSISAAEKGIVRFDEPHSSGTVVMSGLCCGFDGTALV
                     GDGFKPAGFYLYYSHAGPDDGHGHRFNELYRIPLDDLPASGDDGEALGGPGSFTRSQV
                     KDMEFGADDRLYLAWDGTGGGQQLWRFDGPGSFTRLDDDFSDRNRIAFARGSSSMYHL
                     CFWWCPTATGGEGRHELLRRDGFGDSAETRIAGEWMGYDEGSPGMMGVPVGLALSPQG
                     TVAWIADQGNHRIRRVGLPEDPNR"
BASE COUNT          396 a          930 c         1131 g          456 t
ORIGIN      
        1 ggtgacggcc tacgtcaacg ccctcaaggc cggtgatcgg gcacccagca gcgtgaagcg
       61 gatgctggtc tccgtccggg gcctgcaccg gttcctcgcc gaggagaccg agggcgtggc
      121 cgatccgacg caggacgtgg aggtgccgaa gccgccgaag ggcctgccca aggcgctgag
      181 cgaggcagag gtcgagcggt tgctggggtc cgtcaccggc accgacgcgg tggcacgccg
      241 agaccgggcg atcctcgagg tgctctacgg gaccggtgcc cgcatcgggg agctgagcgg
      301 ggtgtcgctc tcggacctcg acctcgacgc cgggctggtg cgcctgttcg gcaagggcgc
      361 caaggagcgc atcgtgccgc tcggtcagtt cgcccagcgg gcgctggtcg actggctcgg
      421 tgaccacggc cggccgctcc tggagcccga gcagtggagg agccgcgacg acgcggatgc
      481 ggtgttcctc aaccgccgcg gcggccgtct gcagcgagga ggcgcctggc tggtggtccg
      541 ccaggccggc gacgccgtcg gcctgggcag ccggctcacg ccgcacgtgc tgcgccactc
      601 gtgcgccacc cacatgctcg accacggagc cgacatccgg accgtccagg agctgctcgg
      661 ccacgcctcg atcagcacca cccaggtgta cacgctcgtg tcgggggagc ggctgcaggc
      721 cgtgtaccag gccgcacacc cccgagcatc cgctgggtag cctgaccgca tggcccagcc
      781 cgacctcacc gccgtccgcg accagctcgt caccgagcag gctgacctcg agcgccagct
      841 ccaggagctg agcggcgacg gcgacgggct cgacgccgac gagaacttcg ccgactcggc
      901 ccaggtggcc gccgagcacg tggagaaccg caccctggcc gcgcagctcc aagaccagct
      961 cgatgacgtc gcggctgcgc tggcccgact cgacgacggc cgctacggca cctgcgaggt
     1021 ctgcgagcag ccggtcacgg cggcccgcct ggaagcgatg cccagcacgc gcttctgcat
     1081 cgaccacgcc tgatggccgg acccgcgcac ctggtcaagc ggttcttcgg ttcgctcgtc
     1141 ccgctgggcg aacccaagaa cgaccgcgcg tgggtgcacg agcacctcct ccccgaggag
     1201 caggagatct ggcgccgcat gtcgcgccag gaccgccgcc acgcggccgg cgtcgcccgc
     1261 cgggtggagc gcgccctcgg gcacgaggcg acgcgaccgg tcatcgcggc cgcgctgctg
     1321 cacgactgcg gcaagtgcgt gtcgcacctc ggcaccaccg gccgcgtcat cgccaccctc
     1381 tcggcgaagg tggccggcca cgagatggca ccggcgtgga gcgagaccac gggcttcacc
     1441 cgccgcgtcg gcctctacct ggagcatccg cggctgggtg ccgacctgct cggcatggcc
     1501 ggctcggccc cgctcacggt ggcctgggcg gccgagcacc acctcccacc cgaggactgg
     1561 acggtccccg ccaacatcgg ccaggtcctc aaggacgccg acgacgactg atcgatgcct
     1621 gacaaccgcg gatcgccggt ggcagctcgg ccggcctggt ccgggtgagc gctgacgtcg
     1681 cgtagggtct ggagttcacg tggtcatggt tgccggtcgg tagctggatg ggtcgatagg
     1741 gcgactcggc gaggtcgacg gggaggggga gcgatgcggg gtggtctgtc gggggtggaa
     1801 ggctggcgcc ggtcgtcgcg gtgtgggcgg gcgctgtgtg tgctggtgtt gctgttggtg
     1861 gcgtcggttc tgccggtggc tgtggtggtg ctgggtggtg gggtgccggc tggtgcggtt
     1921 gatgatttcg gggtgttgtc gacgttggcg gggtcggggg ctgtggggca ggcggatggt
     1981 gctgcggggt cggcgtcgtt cgacagtctg gtggatcttg ctgcgaccta tgacggcagg
     2041 tacctgtatg tgttggaggt gtcgtctggg ggttcgcggg agaacatgat ccggaaggtc
     2101 gacacgggcg atgggtcggt gtcgacgttt gtgacggagt cggaggcgcc ggcgcttcag
     2161 gcgccgaaga gcatccaggt ggaccggttc gggcaggtgt gggtgtcggt gtcgatcagt
     2221 gcggcggaga agggcattgt gcgcttcgat gagccgcact cgtcggggac ggtggtgatg
     2281 tcggggctgt gctgcggttt cgacggcacg gcgttggtcg gtgacgggtt caagccggcg
     2341 ggtttctacc tgtactactc gcatgcgggg cctgacgacg ggcacgggca ccggttcaac
     2401 gagctgtacc ggatcccgct cgatgacctt cctgcatcgg gtgatgacgg tgaggcgctc
     2461 ggtggtccgg gttcgttcac ccggtcgcag gtcaaggaca tggagttcgg cgcggacgac
     2521 cgcctgtatc tggcctggga cgggacgggg ggtgggcagc agctgtggcg gttcgacggt
     2581 ccgggctcgt tcacccgtct cgatgacgac ttcagcgatc ggaaccggat cgcgttcgcg
     2641 aggggcagct cgtcgatgta ccacctgtgc ttctggtggt gcccgactgc gaccggtggc
     2701 gagggaaggc acgagctgct gcgccgggac ggcttcgggg actcggccga gacccggatc
     2761 gccggtgagt ggatgggcta cgacgaaggc tcgccgggga tgatgggcgt cccggtcggg
     2821 ctggccctgt cgccgcaggg gacggtggcg tggatcgccg atcagggcaa ccaccggatc
     2881 cgcagggtcg gtctccccga ggaccccaac cgg
//
LOCUS       DATMFS010000486         4686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17094, whole genome shotgun sequence.
ACCESSION   DATMFS010000486 DATMFS010000000
VERSION     DATMFS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17094"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..671
                     /locus_tag="VNQ33_09155"
     CDS             27..671
                     /locus_tag="VNQ33_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HWJ98315.1"
                     /translation="MTTETAERFSATVRSETAGDHRGAERSAFMEALMGGTLPLAGYV
                     DMLAQHWYAYERLEAQGERLSSDPVAGAFVDTALLRLPALTTDLIELAGADWQAAYPA
                     STATTEYTAAIDRASAWSGGFVAHHYTRYLGDLSGGQHIGRVAARTYDLAPGRGGNFA
                     VFAIDDPAAYREAYRVRLDEAAWDADEQRRMIDEVHEAYRHNRAVFADLDRHGA"
     gene            721..1686
                     /locus_tag="VNQ33_09160"
     CDS             721..1686
                     /locus_tag="VNQ33_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98316.1"
                     /translation="MIPYLLRRVAALAVTVLLALTMMFFLLHASPGSPVDSMGAQVAA
                     DPAAREAITESQGLDRPLLEQYGSYLGGLVRGDLGTSIYDGSSVRTTIATAAPVSAEL
                     GLLAIAVTVIPGLAAGIVAARRHGRAADGVVRVITLVAVSLPSYWLAVLCLIWIGERY
                     PDLLPNAGGFVSFSEDPIANLQVMVLPAIVVGLSGFAIVARSVRSALVEAHGTDEVRF
                     GRAAGLSERDVTRKLALRRAAPPSLAVAGLVVGGLLTGTVLVENVFQIPGLGSLMVTA
                     FTRDDYPLALGCALVTAVVLLSVNLAVDVVLHLLDPRTRPAGRWR"
     gene            1683..2663
                     /locus_tag="VNQ33_09165"
     CDS             1683..2663
                     /locus_tag="VNQ33_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98317.1"
                     /translation="MKRRSWLPQSTALTWVGVVLLGAYVLIATVGRLHGDPTALVADG
                     ALAPSWAHPFGTDSLGRDLLARTAEGAWTSMVISVSAVVLSCLVAVPLGLLAAVRAGR
                     WTDAFVMRIIETTQVVPPFILVLLLLGLTGSRPMEVGPVTVSVNVRIAVCLAIGFVPF
                     FARVTRSAALAELQEPYVEELRRLGVGPREVIGGEVFPNVLPTLAVQALLALAIVVFA
                     EGGLSFLGLGVPPPAPTLGNLIAEAGSQLLGSSWWYALVPGLVLVAGITGLNLLSDAV
                     ADHLLGQGPLPPEPLDPAEDDADADLEAAADPPAAAATLPPAPTAARTTP"
     gene            2660..4246
                     /locus_tag="VNQ33_09170"
     CDS             2660..4246
                     /locus_tag="VNQ33_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98318.1"
                     /translation="MNPDARSGTARATIDRRRFLGLAVGAAIVGPTALAACTSTSSSS
                     RAQKRQADGILRIGVAGAPSNINPLDSGSELTRWLAEPVMESLYTYDDDLKSVPLLAD
                     GEPVVSDDQLTWTIRLKSGITFQNGDPLEAADVVATLDHMLNLSSGSEWITYLLDYVQ
                     RFDAVDEQTFTIGLVKPYGLLRSHLTNLPIAHRDYAERRDAMMGTGPYGLDRFKPGQS
                     FTMSRYDGYHGAKTAFDGIEYTVFTDGSTRLVSLRQGKVDLITAVPYQNLEAVEEDDD
                     LTLIMADAPLDVLTYVMMRAEPFSDDHFRKAVAVSMDRAGVRDRVYGGHATTGQGPIG
                     PAELGWDPDLDVYAAEPDYDQAAELLAQAKTKRRNFRITVGTSQTGRDIAQVLAAGWE
                     QAGLKVEIEQLAGGPWSSAWLSNDYEMLMNTFQSGFTSGPANYLTLAPAHSQNLLSSG
                     YVNPEVDAWMDTVWQTSDDAEREEALRNISRTLAEDAVIFPPAYPKLALAQRKELSPI
                     ATGPLRTSRIQPQLLRFKSS"
     gene            4243..>4686
                     /locus_tag="VNQ33_09175"
     CDS             4243..>4686
                     /locus_tag="VNQ33_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98319.1"
                     /translation="MTSDAAPALLVEQLDIRYRGAAGSVHAVAGVDLRVGRGEVLGVV
                     GESGSGKSSICLALLGLVGDRATRSANRIEIAGHDLTNAREREWRSHRGSTIAFVPQQ
                     PMTALAPTVAVGRQLDWHLGSGAVERHRGALCDLGLQALVDRPDGL"
BASE COUNT          612 a         1877 c         1521 g          676 t
ORIGIN      
        1 tgccgatccc gccacaggag ccatccatga ccaccgagac cgccgaacgc ttctccgcca
       61 ccgtccggtc cgagaccgcg ggcgatcatc gcggcgccga gcgctcggcg ttcatggagg
      121 cgctgatggg cggcacgctg ccgctcgccg gctacgtcga catgctggcc cagcactggt
      181 acgcgtacga gcggctcgag gcgcagggcg agcggctgag ctcggatccg gtggccgggg
      241 cgttcgtcga taccgccctg ctccggctgc cggcgctgac caccgacctg atcgagctgg
      301 ccggggccga ctggcaggcg gcctaccccg cctcgacggc gactaccgag tacacggcgg
      361 ccatcgaccg ggcttcggct tggagcggcg gcttcgtggc gcaccactac acgcggtacc
      421 tgggcgacct gtccggcggc cagcacatcg gccgggtcgc ggcacgcacc tacgacctgg
      481 ccccgggccg cggcggcaac ttcgcagtct tcgccatcga cgatccggcg gcgtaccgcg
      541 aggcctaccg ggtgcgcctc gacgaggccg catgggacgc cgacgagcag cggcgcatga
      601 tcgacgaggt gcacgaggcg taccggcaca accgggccgt gttcgccgac ctcgaccgcc
      661 acggcgcctg acgctcgctg accccgccag caccggcgcg ctcgtcgggg agcgcgcccg
      721 gtgatcccct acctcctccg gcgcgtcgcc gcgctggccg tgaccgtgct gctggcgctc
      781 acgatgatgt tcttcctgct gcacgcatcg ccgggcagcc cggtcgactc gatgggcgcc
      841 caggtggccg cggatccggc agcccgggag gcgatcaccg agtcgcaggg cctcgaccgt
      901 ccgctgctcg agcagtacgg ctcctacctc ggcggcctgg tccgcggcga cctcggcacg
      961 tcgatctacg acggcagctc ggtccgcacc accatcgcca ccgccgcccc cgtctcggcc
     1021 gagctgggcc ttctcgccat cgccgtgacg gtcatccctg gcctggccgc cggcatcgtc
     1081 gcggcccgcc ggcacggccg agccgcggac ggcgtggtcc gcgtcatcac cctcgtcgcc
     1141 gtgtccctgc cgtcgtactg gctcgccgtg ctctgcctca tctggatcgg cgagcgctac
     1201 cccgacctgc tcccgaacgc cggcgggttc gtctcgttct ccgaggaccc catcgccaac
     1261 ctgcaggtga tggtcctccc ggcgatcgtc gtcggcctgt cgggcttcgc catcgtcgcc
     1321 cgttcggtgc gcagcgcgct ggtcgaggcc cacggcaccg acgaggtccg cttcggccgg
     1381 gccgccgggc tgtccgagcg cgacgtcacc cggaagctcg ccctgcgccg ggccgcaccg
     1441 ccgtcgctgg cggtcgccgg gctcgtggtc ggcgggctgc tcaccggcac cgtcttggtg
     1501 gagaacgtct tccagatccc cggcttgggc tcgttgatgg tcaccgcctt cacccgcgac
     1561 gactacccgc tcgccctcgg gtgcgcgctg gtgacggcgg tcgtcctgct gtcggtgaac
     1621 ctcgccgtcg acgtggtgct gcacctgctc gaccctcgga cccgaccggc aggacggtgg
     1681 cggtgaagcg gcgctcctgg ctcccgcagt cgaccgccct cacgtgggtg ggcgtcgtgc
     1741 tgctcggcgc ctacgtcctg atcgcgaccg tcgggcgcct ccacggcgac cccaccgcgc
     1801 tggtggccga tggcgccctc gccccctcct gggcccaccc cttcgggacc gactcgctgg
     1861 ggcgcgacct cctcgcccgc accgccgagg gggcctggac ctcgatggtc atcagcgtga
     1921 gcgccgtggt gctctcgtgc ctcgtggccg tcccgctcgg cctgctcgcc gccgtgcgcg
     1981 ccggccgctg gaccgatgcg ttcgtgatgc ggatcatcga gaccacgcag gtcgtgccgc
     2041 cgttcatcct cgtcctgctg ctcctcggtc tcaccggctc ccggccgatg gaggtcggac
     2101 cggtcacggt ctcggtcaac gtccggatcg cggtgtgcct cgccatcggg ttcgtgccgt
     2161 tcttcgcccg agtcacccgg tcggccgcgc tcgctgaact gcaggagccc tacgtcgagg
     2221 agctccgccg cctcggtgtc ggcccacgcg aggtcatcgg cggcgaggtg ttccccaacg
     2281 tgttgcccac gctcgccgtc caggccctgc tcgccctcgc catcgtcgtg ttcgccgagg
     2341 gcggcctcag cttcctcggc ctcggcgtcc cgccccctgc cccgaccctc ggcaacctca
     2401 tcgccgaggc cggcagccag ctcctcggct cgtcctggtg gtacgccctc gtccccggcc
     2461 tcgtcctcgt cgccggcatc accggcctca acctcctctc ggacgccgtc gccgaccacc
     2521 tcctcggcca gggcccgctt ccgcccgagc cgctcgaccc cgccgaagac gacgccgacg
     2581 ccgacctcga agccgcggcc gatccgcccg ccgcggctgc cacccttcct cctgccccca
     2641 ccgccgcaag gaccacacca tgaaccccga tgcccgctcc gggaccgccc gcgccaccat
     2701 cgaccgccgc cgcttcctcg gcctcgccgt gggtgccgcc atcgtcggcc ccaccgccct
     2761 cgccgcctgc acctccacga gcagcagcag ccgggcccag aagcgccaag ccgacggcat
     2821 cttgcgcatc ggcgtggccg gtgcgcccag caacatcaac ccgctggact ccggctccga
     2881 gctgacccgc tggctcgccg agccggtcat ggagtcgctc tacacctacg acgacgactt
     2941 gaagtcggtc ccgctcctgg ccgacggcga gccggtggtc agcgacgacc agctcacctg
     3001 gaccatcagg ctcaagtccg gcatcacgtt ccagaacggc gatccgctcg aggccgccga
     3061 tgtggtcgcc acgctcgacc acatgctcaa cctgtcgtcg ggcagcgagt ggatcacgta
     3121 cctcctcgac tacgtccagc gcttcgacgc cgtcgacgag cagaccttca ccatcggcct
     3181 ggtcaagccc tacggcctgc tgcgctcgca cctcaccaac ctgcccatcg cccaccggga
     3241 ctacgccgag cgccgcgacg ccatgatggg caccggcccc tacgggctcg accgcttcaa
     3301 gcccggccag agcttcacca tgtcccgcta cgacgggtac cacggcgcca agaccgcctt
     3361 cgacggcatc gagtacacgg tcttcaccga cggctcgacc cgtctcgtga gcctccgcca
     3421 gggcaaggtc gacctcatca ccgccgtccc gtaccagaac ctcgaggcgg tcgaggagga
     3481 cgacgacctg acgctgatca tggccgacgc tccgctcgac gtgttgacct acgtgatgat
     3541 gcgggccgag ccgttcagcg acgaccactt ccgcaaggcc gtcgccgtgt cgatggaccg
     3601 ggccggggtg cgcgaccgcg tgtacggcgg ccacgccacc accggccagg ggcccatcgg
     3661 ccccgccgag ctgggctggg atcccgacct cgacgtgtac gccgccgagc ccgactacga
     3721 ccaggccgcc gagctgctgg cccaggccaa gaccaagcgc cggaacttcc gcatcaccgt
     3781 cggcaccagc cagaccggcc gcgacatcgc ccaggtcctg gccgccggct gggagcaggc
     3841 cgggctgaag gtcgagatcg agcagctcgc cggcggcccg tggtcgtcgg cctggctgtc
     3901 gaacgactac gagatgctga tgaacacctt ccagagcggc ttcacctccg gtccggccaa
     3961 ctacctcacc ctggcaccgg cccactccca gaacctcctg tcgagcgggt acgtcaaccc
     4021 ggaggtcgac gcctggatgg acacggtctg gcagacgagc gacgacgccg agcgggagga
     4081 ggcgctgcgc aacatctcgc ggaccctcgc cgaagacgcc gtgatcttcc cgccggccta
     4141 cccgaagctc gccctggccc agcgcaagga gctgagcccc atcgccaccg ggccgctgcg
     4201 caccagccgc atccagcccc agctcctgcg gttcaaatcg tcgtgaccag cgacgccgca
     4261 cccgccctgc tggtcgagca gctcgacatc cgctaccgcg gcgccgccgg ttcggtgcac
     4321 gcggtcgcgg gggtcgacct gcgcgtcggc cggggcgagg tgctgggcgt cgtgggcgaa
     4381 tcgggcagcg gcaagagcag catctgcctc gccctgctcg ggctcgtggg cgaccgggcg
     4441 acgcggtccg cgaaccgcat cgagatcgcc ggccacgacc tcaccaacgc ccgggagcgg
     4501 gagtggcgct cgcaccgcgg ctcgaccatc gcgttcgtcc cccagcagcc gatgacggcc
     4561 ctcgccccga cggtggccgt gggccggcag ctcgactggc acctggggag cggcgccgtg
     4621 gagcgccacc gcggcgcgct gtgcgacctc gggctccagg ccctcgtcga ccgtcccgac
     4681 ggcctg
//
LOCUS       DATMFS010000487         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_225323, whole genome shotgun sequence.
ACCESSION   DATMFS010000487 DATMFS010000000
VERSION     DATMFS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225323"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VNQ33_09180"
     CDS             complement(<1..1004)
                     /locus_tag="VNQ33_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ98320.1"
                     /translation="MSAAATTTTDTTASTSPLMPTYPPAAVTFTRGRGTRLWDVDGKE
                     YLDLLGGLAVTALGHSHPAVAEALAAQAQTLLHTSNLFGTVPGLEVARTLDRLLGGGG
                     QVFFTNSGAEANECAIKLARRWGGRGKHVVVSALGSFHGRTLATLHATGQPQKWEAFQ
                     PLPEGFRHVAWNDLEALESAIDPSVAAILLEPVQGEGGVNPATAEYFGGVRKLCDERG
                     LLFVVDEVQTGLGRTGEWFGHQHFGVVPDVVTMAKALGNGVPIGACWAKADVAAAFEP
                     GDHATTYGGQPLAAAAARAVLGVMEEIDAPAQAREAGAKLTQVLEADPHVAEVRGLGL
                     L"
     gene            complement(1001..1909)
                     /gene="argB"
                     /locus_tag="VNQ33_09185"
     CDS             complement(1001..1909)
                     /gene="argB"
                     /locus_tag="VNQ33_09185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWJ98321.1"
                     /translation="MADERYVDPQTLAEIDPATRAAILVEALPYIQAFSGKTIVVKFG
                     GNAMVDEALAHSFAADIVLLRAIGLKPVVVHGGGPQIGDLLEKLGIETEFKDGLRVTD
                     ADTLDVARMVLTGSVNADIVSAINQRDRVAVGLSGEDAGLIKAEQRHVDLGYVGDVVE
                     VDRRILDTLLAADFIPVISTIGSDHDGQSYNINADTAAIAIAANLEAEKLIYLTDVSG
                     VLTDVDDPATLVSRLSTARARGLIADGVISGGMIPKIEACLDAVADGVGSAHILDGRI
                     PHVVLLELLTDAGVGTMITRADGAVG"
     gene            complement(1987..3156)
                     /gene="argJ"
                     /locus_tag="VNQ33_09190"
     CDS             complement(1987..3156)
                     /gene="argJ"
                     /locus_tag="VNQ33_09190"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWJ98322.1"
                     /translation="MSVTAAKGFVASGIHCGVKPSGDPDLSLVATADGRPVPAAAVFT
                     DNKMTAAPVLTSDAHLARTHGQAAAVILNSGNANAATGAQGKADAQAMCAAVAERLGC
                     DPHEVLVCSTGLIGIPLPIDIILAGVPDLVAGRRADGDTAAAEAIRTTDTARKVAVAP
                     VGGSGAVVGGMAKGAAMLSPNMATMLAVLTTDAEATNDELREILRGAVSTSFNVLMTD
                     ACRSTNDTVILLASGEAGPVDNDELAAAVGQVCADLASQMAGDAEGATKVVKLTVTGA
                     ASDADAEAGARQIAASALCKCSWFGQDPYWGRLASELGVAGIAMDQEKVSVAYGGVTV
                     AAGGITVAHDEAAVADHLAQRHLDIVCDLGLGDGRFTVLTNDLTHAYIDENTGGS"
BASE COUNT          434 a         1161 c         1181 g          497 t
ORIGIN      
        1 agcaggccga ggccccgcac ctcggccacg tggggatcgg cctcgaggac ctgcgtgagc
       61 ttggcgccgg cttcgcgggc ctgggcgggc gcgtcgatct cctccatcac gccgagcacg
      121 gcgcgggcag cagccgcggc cagaggctgg ccgccgtagg tggtggcgtg atcgccgggc
      181 tcgaacgcgg cggccacgtc cgccttggcc cagcaggcgc cgatggggac gccgttgccg
      241 agggccttgg ccatggtgac cacgtcgggc accacaccga agtgctggtg gccgaaccac
      301 tcccccgtcc ggcccagtcc ggtctgcacc tcgtccacca cgaacagcag gccgcgctcg
      361 tcgcacagct tccggacgcc accgaagtac tcggcggtgg cggggttcac gccgccctcg
      421 ccctgcacgg gctccagcag gatcgccgcc accgacgggt cgatggccga ctccagggcc
      481 tcgaggtcgt tccacgccac gtgccggaag ccctcgggca ggggctggaa cgcctcccac
      541 ttctgcggct ggccggtggc gtgcagcgtg gcgagcgtcc gtccgtggaa cgacccgagc
      601 gcgctgacca cgacgtgctt gccccggcca ccccacctgc gggccagctt gatggcgcac
      661 tcgttggcct cggccccgga gttggtgaag aagacctggc cgccgccgcc cagcagccgg
      721 tccagcgtcc gcgccacctc gaggccgggg acggtgccga acaggttcga cgtgtgcagc
      781 agcgtctggg cctgcgccgc gagcgcttcg gcgaccgcgg gatggctgtg gccgagcgcg
      841 gtgaccgcca ggccgccgag caggtcgagg tactccttgc cgtcgacgtc ccagagccgc
      901 gtgccccggc cccgggtgaa ggtgacggcc gccggggggt acgtcggcat cagcgggctg
      961 gtgctggccg tcgtgtcggt ggtggtggta gcggcggcgc tcatccgacc gctccgtcgg
     1021 cccgggtgat catggtgccg acaccggcat cggtgagcag ctccagcagc accacgtgcg
     1081 ggatgcggcc gtcgaggatg tgggccgatc ccacgccgtc ggccaccgcg tcgaggcagg
     1141 cctcgatctt ggggatcatc cccccggaga tgacgccgtc ggcgatgagg ccccgggcgc
     1201 gggcggtgga cagccgggag accaacgtcg ccggatcgtc cacgtcggtg aggaccccgg
     1261 acacgtcggt caggtagatg agcttctcgg cctcgaggtt ggcggcgatg gcgatggctg
     1321 ccgtgtccgc gttgatgttg tacgactggc cgtcgtggtc cgacccgatg gtcgagatca
     1381 ccgggatgaa gtcggcggcg agcagggtgt cgaggatccg ccggtccacc tccaccacgt
     1441 cgcccacgta gcccaggtcg acgtggcgct gctccgcttt gatcagcccc gcgtcctcgc
     1501 ccgagagccc caccgccacc cggtcgcgct ggttgatggc cgacacgatg tcggcgttga
     1561 cgctgcccgt gaggaccatg cgggcgacat cgagcgtgtc cgcgtcggtg acccgcagcc
     1621 cgtctttgaa ctcggtctcg atccccagct tctccagcag atcaccgatc tggggacccc
     1681 cgccgtgcac caccaccggc ttcagcccga tggcccgcag caagacgatg tcggccgcga
     1741 acgagtgggc cagcgcctcg tcgaccatgg cgttgccccc gaacttgacc acgatggtct
     1801 tgcccgagaa ggcctggatg tacggcagcg cctccaccag gatcgcggcg cgcgtggcgg
     1861 gatcgatctc cgccagcgtc tgcggatcca cgtacctctc gtcagccatc ccgagagtct
     1921 atgcagaaac ctgcatgttc atgcaagaac gagggccggg tgcgcagatc gatctccatc
     1981 gcccggtcac gaaccgccgg tgttctcgtc gatgtaggcg tgggtgaggt cgttggtgag
     2041 gacggtgaag cgaccgtcgc ccaggccgag gtcgcagacg atgtcgaggt ggcgctgggc
     2101 caggtggtcg gccaccgccg cctcgtcgtg ggcgacggtg atcccgcccg cggcgacggt
     2161 cacgcccccg taggccaccg acaccttctc ctggtccatg gcgatgccgg cgacgccgag
     2221 ctcgctggcc aggcggcccc agtacgggtc ctggccgaac cagctgcact tgcacagggc
     2281 gctcgccgcg atctggcggg caccggcctc ggcgtcggca tcgctggcgg cgccggtgac
     2341 cgtgagcttc acgaccttgg tggcgccctc ggcatcgccc gccatctggg aggcgaggtc
     2401 cgcgcagacc tggcccacgg cggccgccag ctcgtcgttg tcgacggggc cggcctcgcc
     2461 gctggccagc aggatcaccg tgtcgttggt ggagcggcag gcgtcggtca tcaacacgtt
     2521 gaagctggtg gagacggcgc cgcgcaggat ctcgcgcagc tcgtcgttgg tggcttcagc
     2581 atcggtggtg agcacggcga gcatcgtcgc catgttgggg ctgagcatcg ccgccccctt
     2641 ggccatgccg ccgacgacgg caccgctccc cccgaccggc gccaccgcca ccttgcgggc
     2701 ggtgtcggtg gtgcggatcg cctcggccgc agcggtgtcg ccgtcggcgc ggcggccggc
     2761 gacgagatcg ggcaccccgg cgaggatgat gtcgatcggc agcgggatcc cgatgagccc
     2821 ggtgctgcag acgaggacct cgtgcggatc gcagccgagc cgctcggcca cggcggcaca
     2881 catggcctgg gcgtcggcct tgccctgcgc tccggtggcg gcgttggcgt tgcccgagtt
     2941 gaggatcacc gccgccgcct gaccgtgcgt gcgggccagg tgggcgtccg acgtgagcac
     3001 cggcgccgcc gtcatcttgt tgtcggtgaa caccgccgcc gccggcaccg gccgcccgtc
     3061 ggccgtcgcc accagcgaca ggtcggggtc gcccgagggc ttgaccccgc agtggatccc
     3121 cgaggcgacg aaccccttcg ctgccgtgac gctcatcgtg ctccgttcgt tcgatttcgg
     3181 gtcacgagtg cacccttgcg gtgcccaggt gacccgattg tgggcggtct cgcggtttcg
     3241 ggtcacgcgg acaccgctgt ggtgcccagg tga
//
LOCUS       DATMFS010000488         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_225367, whole genome shotgun sequence.
ACCESSION   DATMFS010000488 DATMFS010000000
VERSION     DATMFS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225367"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1616)
                     /locus_tag="VNQ33_09195"
     CDS             complement(150..1616)
                     /locus_tag="VNQ33_09195"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HWJ98323.1"
                     /translation="MTSLLTRPRAAAATGVGAALVVSLLGAGAAHAAGPYDDRLVLSQ
                     GHADAFYLHKDASGEPELVIHSDEFGSHAPEQFVIQAKPSVATRTAGAAVAGVLGIEQ
                     GATYYLLPQTNRPGEVFLGFGYDTTAYPAGSIDVTHTITDFDGPGALALWQSGEEGPV
                     EWVNTSKDDWSFTSTANHEHLNWGFTAEGEYTFEVTSTFTDNGTAKTAGPQTYTFFVG
                     EELPSDPEPEPGETTLTLTGAAAHYHTGNVASLTAVQSPAPVSDHFHWFTRAGAGAEW
                     AVVSGAYTETYGFVVTEELQVKAVLYDHDHNAIAETDPVDIHIDDHGNTPGTGPELSV
                     TLPEDEGALVLSVAPGSERSELSDLALNSAADRFVSTGTIGGITVTDTRSANPGWSAS
                     GRVRELVTVDGAVLDGASVGWAPKVDASAGGQTVSAGAAVAPALSGGNGIKAWSVLGS
                     AAAGSSVGTAVLGADLRIEAPTTTEVGEYSGIVLITVI"
     gene            complement(1727..>2633)
                     /locus_tag="VNQ33_09200"
     CDS             complement(1727..>2633)
                     /locus_tag="VNQ33_09200"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HWJ98324.1"
                     /translation="TPDASPVPTGTQTPGPAAAAEQAVPECIPTPVTTTVRPDNVDVV
                     DAGHFDFGPVLDGGTLHALVKDDRAAPASWVDPGTLVFHLGDAAAADAPGGQFAFLGS
                     GRIWQIPLTQQAGVPWLGWNTQHPSIAGHAQGQVTLTLEGLDGPGDLAVYSVDSWGQL
                     GEKYFGTVGGFPRSTSIDVGASGVHVHGIWAFTAPGAYHADLTFAGTIDGERVSATTT
                     LTFFAGAGDPRSAVRERTVTTYVGRTADGEECDTALAATGFADPETAADLSGLAGVLV
                     LVGLAFVGAAALAPRTRERRERPAS"
BASE COUNT          351 a          866 c         1027 g          389 t
ORIGIN      
        1 cgaagaccgt cgcgggggtc agcgcgaccg cgaccgcgag ggcgatgccg gcggagagca
       61 ggcgaaggag atgggacatg tggtctccgg aagacgagat cggatgccgg ccggggatac
      121 gggccggccg gcatccgatc ggatgggtgt cagatcaccg tgatgaggac gatgcccgag
      181 tactcgccga cctcggtggt cgtcggtgcc tcgatgcgca ggtcggcgcc cagcacggcg
      241 gtgccgaccg acgagcccgc ggccgcggag ccgaggacgc tccacgcctt gatgccgttg
      301 ccgccggaga gtgcgggggc gacggcggcg ccggccgaga cggtctggcc gccggcgctc
      361 gcatcgacct tcggcgccca gccgacggac gcaccgtcga gcacggcccc gtcgacggtc
      421 acgagctctc gcacccgtcc cgacgccgac catcccgggt tcgccgagcg ggtgtcggtc
      481 accgtgatgc cgccgatcgt gcccgtcgag acgaagcggt ccgcggcgga gttgagcgcg
      541 agatcggaca gttcgctgcg ctcgctgccg ggcgccacgc tgaggacgag ggcgccctcg
      601 tcctccggca gggtcacgga gagctcgggg ccggtgccgg gggtgttgcc gtggtcgtcg
      661 atgtggatgt cgacgggatc ggtctcggcg atggcgttgt ggtcgtggtc gtagaggacc
      721 gccttgacct ggagttcctc ggtgacgacg aagccgtagg tctcggtgta ggcgccgctg
      781 acgaccgccc actcagcacc ggcaccggcc cgggtgaacc agtggaagtg gtccgagacg
      841 ggcgcgggcg actgcaccgc ggtcagcgac gccacgttgc ccgtgtggta gtgcgcggca
      901 gcccccgtga gggtgagagt cgtctcaccg ggctcgggct cggggtcgga cggcagctcc
      961 tcgccgacga agaacgtgta cgtctgcggg ccggccgtct tcgcggtgcc gttgtctgtg
     1021 aaggtcgagg tgacctcgaa cgtgtactcg ccctctgccg tgaagcccca gttgaggtgc
     1081 tcgtggttgg cggtgctcgt gaacgaccag tcgtccttcg acgtgttcac ccactcgacg
     1141 gggccttcct ctccgctctg ccacagcgcg agcgcaccgg ggccgtcgaa gtccgtgatc
     1201 gtgtgggtca catcgatcga gcccgccggg tacgccgtcg tgtcgtagcc gaagccgagg
     1261 aagacctctc ccggccggtt ggtctgcggc agcaggtagt aggtcgcgcc ctgctcgatg
     1321 ccgagcactc cggcgacggc ggcgccggca gtgcgggtgg ccaccgacgg cttggcctgg
     1381 atcacgaact gctcgggcgc atggctgccg aactcgtcgg agtggatgac gagctcgggc
     1441 tcgccgctcg catccttgtg caggtagaag gcgtcggcgt ggccctgtga caggacgagc
     1501 ctgtcgtcgt agggccctgc cgcatgcgcg gcgcccgctc cgaggaggga cacgaccagc
     1561 gccgccccca ctccggtggc ggccgcggcg cgcggcctgg tgaggagaga tgtcatgtcg
     1621 gtgacatcct ttctattctt cggtggccgt tcggccgtgg tgtgtgatcg ggaggcggtg
     1681 caccctgccg ggtgcggcat cctcccgatc acggaggatg ggaagctcag gacgcgggac
     1741 gctcgcggcg ctcgcgggtg cggggagcga gtgcggccgc gccgacgaag gcgaggccga
     1801 ccagcaccag aacgccggcc aggcccgaca ggtcggcggc ggtctcgggg tctgcgaagc
     1861 cggtcgccgc cagtgccgtg tcgcactcct cgccgtccgc cgtgcggccg acgtaggtcg
     1921 tgacggtgcg ctcgcggacg gccgagcgag gatcgcctgc ccccgcgaag aacgtcagcg
     1981 tcgtggtggc gctgacgcgc tcgccgtcga tcgttcccgc gaaggtgagg tcggcgtggt
     2041 acgcgccggg cgcggtgaag gcccagatgc cgtgcacgtg cacgccggag gcgccgacgt
     2101 cgatgctcgt gctgcgcggg aagccgccga ccgtgccgaa gtacttctcg ccgagctgcc
     2161 cccacgagtc gacggagtac accgcgaggt ccccggggcc gtcgaggccc tcgagcgtga
     2221 gcgtcacctg tccctgggcg tgtccggcga tcgagggatg ctgggtgttc cagcccagcc
     2281 aggggacgcc ggcctgctgc gtgagcggga tctgccagat ccggcccgag ccgaggaagg
     2341 cgaactggcc gcccggggca tcggcggcgg ccgcgtcgcc caggtggaac acgagggtgc
     2401 cggggtcgac ccagctcgcg ggggcggcgc ggtcgtcctt cacgagtgcg tgcagggtgc
     2461 cgccgtccag gacggggccg aagtcgaagt gtcctgcgtc gacgacgtcg acgttgtcgg
     2521 gacgcacggt cgtcgtgacg ggagtcggga tgcactccgg gacggcctgc tcggccgcgg
     2581 ccgccgggcc gggcgtctgc gtcccggtcg gcacaggcga cgcgtcggga gtc
//
LOCUS       DATMFS010000489         4957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17238, whole genome shotgun sequence.
ACCESSION   DATMFS010000489 DATMFS010000000
VERSION     DATMFS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17238"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VNQ33_09205"
     CDS             <1..1552
                     /locus_tag="VNQ33_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ98325.1"
                     /translation="RVLDPALRPTDALSQVAGITFRDGGRITSTAERSRHESLEDFPS
                     PYLTGEFDHVDSSGWQWPSIGTNRGCPYSCSFCDWGSATRSRVRDFPMERVLGELQWL
                     SDHGLPHWMINDANFGIHRRDVEIAKFIVELRRAGGVPEALYMSYAKNTVKYLAEIIK
                     MITEAGIIGEAALALQTRDPETLRAIKRSNIRVEKFDDLAEEFRRYGLPLVTDLIIGL
                     PGQTVESFLTDMQYCVDNDVTPRFFVALNLPNAEFNNPEYQRKFSIVLGDGDVVVETS
                     SFTRSDRDRMMRLRMAYRSFEHFALLRHVFRYLQWDHGIAEATVIERIEAVTAASPER
                     YPLLSWVQGYFDMFLIPPFGWPPFFAEVATFVSEEFGIEDDAVLRTVFEVQMAILPEP
                     NRTFPSTVHLDHDYVTWFEAHGRQRPDGPRLGELPPGELVVTGDPCQCCVTGVGRQCD
                     VRPHELIIAPFWAALHWELDSPVQRIVPAVAVSNSASQQQDPAAGPGGPAADPEGVGA
                     TASVDATV"
     gene            complement(1884..2945)
                     /locus_tag="VNQ33_09210"
     CDS             complement(1884..2945)
                     /locus_tag="VNQ33_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWJ98326.1"
                     /translation="MGSVSMSQGMTMEREAIGTVRPTLLDERAQAELERDGYTIIDLL
                     DADQVTELHRQYEAAGPQPADPHEACIDTFFSPDLAYKAKIHETVERVMGPALESHLD
                     RQHALSFSFANKWSSEKSSFGLHQDISVVDETKHRSVEVWCPLVDTDEENGQLWVVPG
                     SHKWRSVNRPIHRDRPPFWGVKERIVARHSVPLPLRAGQAIVFDHATYHFSYANRSDQ
                     PRLTAVCDLRPEEAEHVHYVRPQDSDEIDVYAISDRFWVEVNPFNVSDEIQKFEHVGT
                     VEYDVEPITDADLDQFVAEGRAVDHEPLQLGPINPTDPWCHRCGTTEGVEGEVDAWLG
                     NTILACPSCASADDPSAVI"
     gene            complement(2999..4408)
                     /locus_tag="VNQ33_09215"
     CDS             complement(2999..4408)
                     /locus_tag="VNQ33_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ98327.1"
                     /translation="MTRTAPACRAPSVSMYLDPLGVVRPCCVNPFYVLGRITEQSLAD
                     IWNGERTRQLRERLGHGDLSGGCDACRGPDRGDGEDHSSFARTFDVFPLAGGEPDVTW
                     PVQLELALSNTCNLQCTMCSGTLSSAIRAGREGLPPLPKVYDDRFFEELRPFLAHAER
                     VTLLGGEPLLARETWRVVDLLEEVGNQPRLHVTTNGMIWNDKVERLLTRFPTDVVVSV
                     DAVDPDLLRSIRIGIEPERVMANLERFWEHAPRNGGSLAIACCLMTESAPDLRAVLAL
                     ADRLDVDVFLNDVAWPAVSAYWSLDHEARAALVADLRSVEALGPPLHRNAAVWEDALS
                     RIEAAPEPVAVELRVTSRSPELGPPVRFEVNDKHQLVAIEPDPSDVFGVDLTAVVGTP
                     VGEFREPFHAVHGPLLRSQVQRNEGGSQVWTLDMGSPEAVRRLRATFEPGATTGGTWV
                     LTLHELSPIDTLATGPVAN"
BASE COUNT          774 a         1689 c         1714 g          780 t
ORIGIN      
        1 ccgcgtgctc gatcccgctc tccgccccac cgacgcactg tcgcaggtcg ccggcatcac
       61 ctttcgagac ggcgggcgaa tcacctccac ggccgagcgc tcaaggcacg agtcgctcga
      121 ggacttcccg tcgccgtacc tcacgggcga gttcgatcac gtcgacagct cggggtggca
      181 gtggccatcg atcgggacca accgcggctg cccctacagc tgctcgttct gcgactgggg
      241 ttccgcgacc cggtcgcggg tgcgcgactt ccccatggaa cgggtcctgg gcgagctcca
      301 gtggctttcg gaccacggcc tgccgcactg gatgatcaac gacgccaact tcggcatcca
      361 ccgacgtgac gtcgagatcg caaagttcat cgtggagctc cggcgtgccg gcggagttcc
      421 cgaagcgctc tacatgagct acgcgaagaa caccgtcaag tacctcgctg agatcatcaa
      481 gatgatcacc gaggcgggga tcatcggtga ggcagccctc gcgctgcaga cgcgagaccc
      541 cgagacgctc cgagcgatca agaggtcgaa catccgggtc gagaagttcg acgatctcgc
      601 tgaggagttc cgtcgatacg gcctgcccct cgtcaccgat ctgatcatcg gactcccagg
      661 gcagacggtc gagtccttcc tcacggacat gcagtactgc gtggacaacg acgtcacccc
      721 ccggttcttc gtggcgctca acctgccgaa cgccgagttc aacaatcccg agtaccaacg
      781 gaagttctcc atcgtcctcg gggacggaga tgtcgtggtg gagacctcga gcttcacccg
      841 gtcggatcga gatcggatga tgcgcctgcg catggcgtac cgctcgttcg agcacttcgc
      901 actgctccgt cacgtcttcc gctacctcca gtgggatcac ggcatcgccg aagcgacggt
      961 gatcgaacgg atcgaagcgg tgacggcagc gtcgcccgag cggtacccgc tgctctcgtg
     1021 ggtgcaggga tacttcgaca tgttcctcat accgccgttc gggtggccgc ccttcttcgc
     1081 cgaggtcgcc accttcgtat ccgaggagtt cggcatcgaa gacgacgcgg tgctccgcac
     1141 ggtgttcgag gtccagatgg cgatcctgcc cgagccgaac cgcaccttcc cctcgaccgt
     1201 gcacctcgac cacgactacg tgacttggtt cgaggcgcat ggccgccagc ggccagacgg
     1261 gccccgcctc ggggagcttc cgcccggcga gctcgtggtc accggggacc cgtgccagtg
     1321 ctgcgtgacc ggggtcggcc gccagtgcga cgtccgcccc cacgagctga tcatcgcgcc
     1381 cttctgggct gccctccact gggagctgga ctcgcccgtc cagcggatcg tgccggcggt
     1441 ggcggtcagc aacagcgcct cgcagcagca ggaccccgcg gcaggtcccg gaggtccggc
     1501 ggcggatcca gaaggcgtgg gtgctaccgc gtcggtggat gcaacggtgt aggactcggc
     1561 gagtcgggcg cctggccgcg cacggcgcca gcgacctccg cggccgcggc gcggctcacg
     1621 ccgggccacc gcccccggcc agctccgccc gggacctcat cggcggtggg taccgtcgcg
     1681 ggccccgcgt caccaccgag ctgaccatcc gccgagcgct cggcgcatca gatccggcag
     1741 gccgcgtgat ggccttggac gcgactcggg actgaccgaa ggcctcggac tcgcctttgg
     1801 tcagtcccga gtcgaacttc tgtccacctg ccgtcccacg atcagggacg cttggcagct
     1861 gacgggcgcg ggcccgggtg cgatcagatg acggccgagg ggtcgtcagc tgacgcgcag
     1921 gacgggcacg cgaggatcgt gttcccgagc caggcgtcga cttcgccttc gaccccttcg
     1981 gtggtaccgc aacgatggca ccacgggtcg gtcgggttga tcggaccgag ctgcagcggc
     2041 tcgtgatcga ccgctcggcc ctcggcgacg aactggtcga ggtcggcgtc ggtgatcggc
     2101 tcgacgtcgt actccacggt gccgacgtgc tcgaacttct ggatctcgtc agacacgttg
     2161 aacgggttca cctcgaccca gaaccggtcg gagatggcgt acacgtcgat ctcatcgctg
     2221 tcctgcgggc gcacgtagtg cacgtgctcg gcctcttcag gacggaggtc gcacaccgcc
     2281 gtgaggcggg gctgatccga tcggttggcg tacgagaagt ggtaggtggc gtggtcgaag
     2341 acgatcgcct ggccagcacg cagcgggagc ggcacgctgt gacgagccac gatgcgctcc
     2401 ttgacgcccc agaacggggg ccggtcgcgg tggatcgggc ggttcaccga acgccacttg
     2461 tggctgccgg gcacgaccca gagctggccg ttctcctcgt cggtgtccac cagagggcac
     2521 cagacctcga cggagcggtg cttggtctcg tccaccacgg agatgtcctg gtggaggccg
     2581 aagctgctct tctcgctcga ccacttgttg gcgaagctga aggagagggc gtgctggcgg
     2641 tccaggtggc tctcgagggc tgggcccatc acccgttcga ccgtctcgtg gatcttggcc
     2701 ttgtacgcca ggtccgggga gaagaacgtg tcgatgcagg cctcgtgcgg gtcggccggc
     2761 tgggggcccg cggcctcgta ctggcggtgg agctccgtga cctggtccgc atcgaggagg
     2821 tcgatgatcg tgtacccgtc gcgctcgagc tcggcctggg cccgctcgtc cagcaacgtc
     2881 ggacgcaccg tcccgatcgc ctcgcgctcc atcgtcatgc cttggctcat gctcacgctc
     2941 cccatcagta atcctggcta ggctccgggg acactaccac gggaaccgcg cctgtcgctc
     3001 agttggcgac ggggccggtc gccagggtgt cgatcggcga cagttcgtga agcgtcagca
     3061 cccaggtacc acccgtcgtg gctccgggct cgaacgtggc gcgaaggcgg cgaacggcct
     3121 cgggcgaccc catgtcgagc gtccacacct ggctgccgcc ctcgttgcgt tggacctggg
     3181 accgcaggag cgggccgtgc accgcgtgga agggctcgcg gaactcgcct acgggcgtcc
     3241 ccaccaccgc ggtgaggtcg acgccgaaca cgtcggacgg gtcgggttcg atggcgacca
     3301 gttggtgctt gtcgttgacc tcgaagcgaa ccggcggacc gagctctggt gaccgggacg
     3361 tcacgcggag ctccaccgcg accggctccg gggcagcttc gatccgactg agggcgtcct
     3421 cccacacggc cgcgttgcgg tgcagcggag gaccgagggc ctcgaccgag cggaggtcgg
     3481 cgaccagggc cgcacgcgcc tcgtggtcga gcgaccagta ggcggagaca gccggccacg
     3541 ccacgtcgtt caagaacacg tcgacgtcaa gccgatcggc gagggcgagc acggctcgca
     3601 gatcgggcgc gctctcggtc atcaagcagc acgcgatggc gagcgacccg ccgttgcggg
     3661 gcgcgtgctc ccagaagcgc tccaggttgg ccatgacccg ctccggctcg atgccgatgc
     3721 ggatggagcg gagcaggtcc ggatcgaccg cgtcgaccga cacgaccacg tcggtgggga
     3781 accgggtgag caagcgctcg accttgtcgt tccagatcat cccgttcgtg gtcacgtgga
     3841 ggcgcggctg gttgcccacc tcctcgagca gatcgaccac ccgccacgtc tcgcgggcga
     3901 gcagcggctc gccgccgagc agcgtgacgc gctcggcgtg ggcgaggaag ggccgcagtt
     3961 cctcgaagaa gcggtcgtcg tacaccttcg gaagcggtgg caacccctct cgaccggcgc
     4021 ggatggccga tgacagcgtg cccgagcaca tcgtgcactg gaggttgcac gtgttcgaga
     4081 gcgccagctc gagctggacg ggccaggtga catctggctc gccgccggcg agcgggaaca
     4141 cgtcgaaggt gcgggcgaac gagctgtggt cctcgccgtc gccgcggtcg ggacctcggc
     4201 aggcatcgca gccgccgctc aggtcaccgt ggccgagccg ttcgcgcagt tgccgggtgc
     4261 gctcgccgtt ccagatgtcg gccagcgact gctcggtgat gcggccgagc acgtagaagg
     4321 ggttgacgca gcacggtcgg acgaccccca acggatcgag gtacatcgac accgacggtg
     4381 ctcggcacgc cggcgcggtc cgggtcatgg ccatggagtg taccggccgt cttggatgag
     4441 ccggcagagg cccgccgcac gccatctctc tcgtgggtcc gtgccatctc gtagatccgt
     4501 gccaccggca gaccgcccgg cctccgacct ggccgagccc ccgaccggtc ccaggtcggg
     4561 gtggtcgacc gatccgtcgc ttccaccggc tcgcgagctc atcagccgac gggtgcgcgc
     4621 gtcggcctcc ttcggcgtgc gagggtctcg aacgggtgtt ggctcagatc ggaggtagcg
     4681 gtctatcctg accgcctggc tggagggcgg gagcccacca gggcccccgg ccaaggacga
     4741 ccgtcctcga acagggggcc gttgcgccat ctgccatgcc agcgaaccct ggcctcgtac
     4801 gaggagcgac atgcagaatc tgttccgacg aattcgccgc cggtgggcag gggcgccgtc
     4861 acgaccgcgt gtgaccgaag caggagcaac ggaagaggca ccggagctcg aggccccccc
     4921 gacggcggtc gatgcggcgg atgccgacga cggagcg
//
LOCUS       DATMFS010000490         5458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_62693, whole genome shotgun sequence.
ACCESSION   DATMFS010000490 DATMFS010000000
VERSION     DATMFS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62693"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..652
                     /gene="rplE"
                     /locus_tag="VNQ33_09220"
     CDS             77..652
                     /gene="rplE"
                     /locus_tag="VNQ33_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWJ98328.1"
                     /translation="MSTETIEIPRLKAKYHDEIRDQLQADLGLGNVMQVPRLEKIVVN
                     MGVGGAVGQASLLEHAVTDLTLITGQKPLITRARKSIANFKLREGNAIGAKVTLRGDR
                     MWEFLDRLIALAIPRIRDFRGLPANSFDGRGNYTFGVTEQLIFPEIDYDKIDQTRGMD
                     ITIVTTAVDNAGGKALLDAFGFPFKREGQAS"
     gene            652..837
                     /locus_tag="VNQ33_09225"
     CDS             652..837
                     /locus_tag="VNQ33_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWJ98329.1"
                     /translation="MAKKALINKANKKPKFKVRGYTRCQKCGRPHSVYRKFKLCRICL
                     RDMAHAGEIPGVTKASW"
     gene            852..1256
                     /gene="rpsH"
                     /locus_tag="VNQ33_09230"
     CDS             852..1256
                     /gene="rpsH"
                     /locus_tag="VNQ33_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWJ98330.1"
                     /translation="MTMTDPIADMLTRVRNANVAMHDEVKMPSSKKKEALAALLKKEG
                     YIEDFSVADNGSRPGSTLTVTMKYSPERARTISGLRRVSKPGLRVYKPAQEVPRVLGG
                     LGVAVVSTSQGLMTDREARKRNVGGEVLCFVW"
     gene            1265..1804
                     /gene="rplF"
                     /locus_tag="VNQ33_09235"
     CDS             1265..1804
                     /gene="rplF"
                     /locus_tag="VNQ33_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWJ98331.1"
                     /translation="MSRIGKAPIPVPSGVDVTVADRTITVKGPKGTLDRTIPGTITIR
                     QEGAELLVERPDDERQSRAMHGLVRSLVNNMVVGVTDGFTKQLEIIGVGYRATAKGQG
                     TLDLALGFSHPVEVKAPAGVTFDVPSPNRIDVKGSDKELVGQVAADIRKLRKPEPYKG
                     KGVRYLGEHVVRKAGKSGK"
     gene            1810..2172
                     /gene="rplR"
                     /locus_tag="VNQ33_09240"
     CDS             1810..2172
                     /gene="rplR"
                     /locus_tag="VNQ33_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWJ98332.1"
                     /translation="MSDSAKQKREARIRRHRRVRKKVRGTAERPRLAVFRSNKHITAQ
                     VIDDRAGHTLASASTTEAALAGNGGNVDAATKVGELVAERATAAGITTVVFDRGGNLY
                     HGRIAALAEAARKGGLEF"
     gene            2172..2735
                     /gene="rpsE"
                     /locus_tag="VNQ33_09245"
     CDS             2172..2735
                     /gene="rpsE"
                     /locus_tag="VNQ33_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWJ98333.1"
                     /translation="MATNSNDAQLRESRVININRVAKVVKGGRRFSFTALVVIGDGAG
                     RVGLGYGKAKEVPLAIQKGTEEARKNLFSVPLAGSTITHPTIGIMGAGRVMLKPAAPG
                     TGVIAGGAARAILEEAGIHDVLCKSLGSANHINVARATIAGLKSLKRPDEIARLRGLS
                     PEEFVPKGLLDAYNESERGPVQFEEVK"
     gene            2738..2917
                     /gene="rpmD"
                     /locus_tag="VNQ33_09250"
     CDS             2738..2917
                     /gene="rpmD"
                     /locus_tag="VNQ33_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041604457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWJ98334.1"
                     /translation="MAELKVTQIRSTIGVKPKTRGTVRALGLRGIGQSNTLPDRPEIR
                     GMIARVPHLITVEEV"
     gene            2920..3408
                     /gene="rplO"
                     /locus_tag="VNQ33_09255"
     CDS             2920..3408
                     /gene="rplO"
                     /locus_tag="VNQ33_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWJ98335.1"
                     /translation="MKVHDLQPAPGSNKGRRRVGRGIAGKGGKTAGRGTKGQAARGQI
                     PASFEGGQMPLHMRVPKLKGFTNPFRVEYQAVNLDTLEATGLDEVSPEGLHAKGLVSK
                     GSLVKVLGRGQVTRAITVRAHAFSKSAEEAITAAGGTVERLALPWGEGRPPAKGNQFT
                     NR"
     gene            3454..4833
                     /gene="secY"
                     /locus_tag="VNQ33_09260"
     CDS             3454..4833
                     /gene="secY"
                     /locus_tag="VNQ33_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWJ98336.1"
                     /translation="MSTLRNIFKIPELRNKVLFTLLMIAVYRLGSHVPVPGIDLGQIK
                     EIQDKADSSGVLNLLQTFSGGAITQFAVFALGIMPYITSSIIMQILAVVIPKLEQWQD
                     QGAVGQRKITQWTRYLGVAIALLQATGITYLFHTGGGGFGANVNTTGLDVFGDNYNAG
                     RFVLVVLTLTTGFALLMWIGEVITQRGVGNGMSLLIFASVVSQMPRNFITVQQEKNTF
                     TMIAVALMFIVLMVGIVFVEQGVRRIPVQFAKRQVGRKMFGGQSTYIPLKVNGAGVIS
                     IIFASSVLYLPVIISQILPVGDATAENPNIWWDIQQFVDQHLTRSTSPAYLVTYGVLI
                     ILFAYFYTAIQFDPHKQADQIRKQGGFIPGIRPGYQTERYLAKILSRITLPGALFVAG
                     IALIPAFVLGKVLGDTSGGQRLGFFGISILIAVGVAIETVKQVDGQLMMRNYEGFLNT
                     KPTKTKTTK"
     gene            4838..>5458
                     /locus_tag="VNQ33_09265"
     CDS             4838..>5458
                     /locus_tag="VNQ33_09265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWJ98337.1"
                     /translation="MTDGVRLVMLGRQGAGKGTQAIRLAQHYVVPHISTGDMLRAAVA
                     AGTEFGLRAKSVMDGGGLVGDDIMIGVVDERLNESDTTKRGYILDGFPRTVPQGISLE
                     AITEARPLDVAVDLEVPEDVVLERITKRRVCKTCGHIYSVDAPPKNGWICDVDGGEVI
                     QRADDTEEAVRKRLDLYTAETKPLVPFYAERGLLVEVDGLGTADEVY"
BASE COUNT          975 a         1999 c         1700 g          784 t
ORIGIN      
        1 aggccacccg tgtgggctac caggtcgtcg acggcaagaa ggtgcgcgtc tgcaagcgca
       61 cgggggtgga gatcccatga gcaccgagac catcgagatc cctcgcctga aggcgaagta
      121 ccacgacgag atccgcgacc agctccaggc tgacctgggc ctcggcaacg tgatgcaggt
      181 gccccgcctc gagaagatcg tggtgaacat gggcgtcggc ggtgccgtcg gccaggcctc
      241 gctcctcgag cacgccgtca ccgacctcac cttgatcacc ggccagaagc cgctgatcac
      301 ccgggcccgc aagtccatcg ccaacttcaa gctccgtgaa ggcaacgcca tcggcgccaa
      361 ggtcacgctg cgcggcgatc gcatgtggga gttcctggac cggctgatcg ccctggcgat
      421 cccccgcatc cgtgacttcc gcggcctccc ggccaacagc ttcgacggcc ggggcaacta
      481 cacgttcggc gtgaccgagc agctgatctt cccggagatc gactacgaca agatcgacca
      541 gacccgaggc atggacatca ccatcgtgac caccgccgtc gacaacgcag gcggcaaggc
      601 cctgctcgac gccttcgggt tcccgttcaa gcgcgaaggg caggccagct gatggcgaag
      661 aaggctctga tcaacaaggc caacaagaag cccaagttca aggtgcgggg ctacacgcgg
      721 tgccagaagt gcgggcgacc gcactcggtg taccggaagt tcaagctgtg ccgcatctgc
      781 ctgcgggaca tggcgcacgc cggggagatc cccggcgtga ccaaggcgag ctggtgaggg
      841 aggactgaac catgaccatg actgatccca tcgccgacat gctcacccgt gtccgcaacg
      901 ccaacgtcgc catgcacgac gaggtgaaga tgccgtcttc caagaagaag gaggcccttg
      961 cggcgctcct gaagaaggag ggctacatcg aggacttcag cgtcgccgac aacggcagcc
     1021 gtcccggcag caccctcacc gtcaccatga agtactcgcc cgagcgggcc cggaccatct
     1081 ccggcctccg ccgggtgtcc aagcccggtc tccgggtgta caagccggcc caggaggtcc
     1141 cccgcgtcct cggtgggctc ggtgttgccg tcgtctcgac cagccagggc ctgatgaccg
     1201 accgcgaggc gcgcaagcgc aacgtgggcg gcgaagtcct gtgcttcgtc tggtaggagc
     1261 acccatgtcc cgcatcggca aagcacccat ccccgtgccc agcggcgtcg acgtcaccgt
     1321 cgccgaccgc accatcacgg tcaagggccc caagggcacc ctcgaccgca ccatcccggg
     1381 caccatcacc atccgccagg aaggcgccga gctgctcgtc gagcggcccg acgacgagcg
     1441 ccagagccgc gccatgcacg gcctggtccg ctccctggtc aacaacatgg tcgtcggcgt
     1501 gaccgacggc ttcaccaagc agctcgagat catcggcgtc ggctaccgcg ccaccgccaa
     1561 gggccaaggc accctcgatc tcgccctcgg gttcagccac cccgtcgagg tcaaggcgcc
     1621 cgccggcgtc accttcgacg tgccgagccc caaccgcatc gacgtcaagg gctccgacaa
     1681 ggagctggtc ggacaggtcg ccgccgacat ccgcaagctg cgcaagcccg agccctacaa
     1741 gggcaagggc gtccgctacc tcggcgagca cgtcgtccgc aaggccggaa agtccgggaa
     1801 gtaggaacca tgagcgattc agcgaagcag aagcgcgaag cccgcatccg ccgccaccgc
     1861 cgggtgcgca agaaggtccg ggggaccgcc gagcgcccgc gcctggccgt gttccggtcc
     1921 aacaagcaca tcaccgccca ggtcatcgac gaccgagccg gccacaccct cgcctcggcc
     1981 tccaccaccg aggccgcgct ggccggcaac gggggcaacg tcgacgccgc caccaaggtc
     2041 ggtgagctcg tcgccgagcg ggccaccgcc gccggcatca ccaccgtggt cttcgaccgg
     2101 ggtggcaacc tctaccacgg ccgcatcgcg gcgctcgccg aagccgctcg caagggcgga
     2161 ctggagttct gatggctacc aactcgaacg acgctcagct ccgtgagtcg cgcgtcatca
     2221 acatcaaccg cgtggccaag gtggtcaagg gcggccgtcg cttctccttc accgccctcg
     2281 tcgtgatcgg cgacggcgcc ggtcgcgtgg gcctcggcta cggcaaggcg aaggaggtgc
     2341 ccctcgccat ccagaagggc accgaggagg ctcgcaagaa cctcttctcc gtcccgctgg
     2401 ccggctccac catcacccac cccaccatcg ggatcatggg tgccggtcgg gtcatgctga
     2461 agccagccgc ccctggtacc ggcgtcatcg ccggcggtgc cgcccgtgcc atcctcgaag
     2521 aggccggcat ccacgacgtg ctctgcaagt cgctcggctc cgccaaccac atcaacgtcg
     2581 cccgcgccac catcgccggc ctcaagtcgc tcaagcgccc cgacgagatc gcccgcctcc
     2641 gcggcctctc gccggaggag ttcgtgccca agggcctgct cgacgcgtac aacgagtccg
     2701 agcgcgggcc cgtgcagttc gaagaggtga agtgacgatg gccgagctca aggtcaccca
     2761 gatccgatcc accatcggcg tcaagcccaa gacccgcggc accgtccgcg ccctgggcct
     2821 ccgtgggatc ggccagtcca acaccctccc cgaccgtccc gagatccgcg ggatgatcgc
     2881 ccgggtgccc cacctgatca ccgtcgaaga ggtctgatca tgaaggtcca tgatctccag
     2941 cccgctccgg gctccaacaa gggccgccgc cgcgtcggcc gcggcatcgc cggcaagggc
     3001 ggcaagaccg ccggccgggg caccaagggc caggccgctc gcggccagat ccccgcctcg
     3061 ttcgaaggcg gccagatgcc cctgcacatg cgggtgccga agctcaaggg cttcaccaac
     3121 ccgttccgcg tcgagtacca ggccgtgaac ctcgacacgc tcgaggccac cggactcgac
     3181 gaggtcagcc ccgagggact gcacgccaag ggcctcgtgt ccaagggctc cctcgtcaag
     3241 gtgctcggcc gtggccaggt cacccgggcc atcacggtcc gggcccacgc gttctccaag
     3301 tccgccgaag aagccatcac ggcggccggc ggtacggtgg agcgccttgc cctcccgtgg
     3361 ggcgaaggcc gcccgcccgc caagggcaac cagttcacca accgctgacc cgtcggcctc
     3421 cgggccgccc cgatcgctga tcaggagcgc gagttgtcga ccctgcgcaa catcttcaag
     3481 atcccggagc tccgcaacaa ggtgctgttc accttgctga tgatcgccgt gtaccgactg
     3541 ggctcgcacg tcccggtgcc gggcatcgac ctggggcaga tcaaggagat ccaggacaaa
     3601 gcggactcca gcggcgtgct gaacctgctg cagaccttct cgggcggggc catcacccag
     3661 ttcgcggtgt tcgcgctcgg gatcatgccc tacatcacct cgtcgatcat catgcagatc
     3721 ctcgccgtgg tgatccccaa gctcgagcag tggcaggacc agggggcggt gggccagcgc
     3781 aagatcaccc agtggacccg ctacctcggt gtggccatcg ccctgctcca ggccaccggc
     3841 atcacctacc tgttccacac cggcggcggc gggttcggtg ccaacgtcaa caccaccggg
     3901 ctcgacgtgt tcggcgacaa ctacaacgcc gggcgcttcg tcctcgtggt gctcacgctc
     3961 accaccggct tcgcgctgct gatgtggatc ggcgaggtca tcacccagcg cggcgtcggc
     4021 aacggcatgt cgctgctgat cttcgcctcc gtcgtgagcc agatgccccg caacttcatc
     4081 acggtgcagc aggagaagaa caccttcacg atgatcgccg tggcgctcat gttcatcgtg
     4141 ttgatggtgg gcatcgtgtt cgtggagcag ggcgtgcgcc gcatcccggt gcagttcgcc
     4201 aagcgccagg tgggacgcaa gatgttcggc ggccagagca cctacatccc gctcaaggtc
     4261 aacggcgccg gcgtgatctc gatcatcttc gcaagctccg tgctgtacct gccggtgatc
     4321 atctcccaga tcctcccggt cggcgatgcc accgccgaga acccgaacat ctggtgggac
     4381 atccagcagt tcgtcgacca gcacctgacc cggtcgacct cgcccgccta cctggtgacc
     4441 tacggcgtgc tcatcatctt gttcgcctac ttctacacgg ccatccagtt cgacccgcac
     4501 aagcaggccg accagatccg caagcaaggc ggcttcatcc ccggcatccg ccccggctac
     4561 cagaccgagc gctacctggc gaagatcctc tcccggatca cgctgcccgg cgccttgttc
     4621 gtggccggca tcgccctcat cccggccttc gtcttgggca aggtcctggg cgacacctcc
     4681 ggtggccagc gcctcggctt cttcggcatc tcgatcctca tcgccgtcgg cgtggccatc
     4741 gagacggtca agcaggtcga cggccagctg atgatgcgca actacgaggg cttcttgaac
     4801 accaagccga ccaagaccaa gacgacgaag tagcgccatg accgacggcg tccgtctcgt
     4861 catgctcggc cgccagggcg cgggcaaggg aacccaggcc atccgcctgg cccagcacta
     4921 cgtggtcccg cacatctcga ccggcgacat gctccgcgcc gccgtggccg ccggcaccga
     4981 gttcggcctg cgcgccaagt ccgtgatgga cggcggcggg ctggtgggcg acgacatcat
     5041 gatcggcgtg gtcgacgagc ggctgaacga gagcgacacg accaagcgcg gctacatcct
     5101 cgacggcttc ccgcggaccg ttccccaggg catctcgctc gaggccatca ccgaggcccg
     5161 cccgctcgac gtggcggtcg acctggaggt gcccgaagac gtcgtgctcg agcgcatcac
     5221 caagcgccgg gtctgcaaga cctgcggcca catctactcc gtggacgcgc cgccgaagaa
     5281 cggctggatc tgcgacgtcg acggcggcga ggtcatccag cgcgccgacg acaccgaaga
     5341 ggcggtgcgc aagcgcctcg acctgtacac cgccgagacc aagccgctgg tgcccttcta
     5401 cgccgagcgc gggctgctgg tcgaggtcga cggcctgggc accgcggacg aggtgtac
//
LOCUS       DATMFS010000491         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17273, whole genome shotgun sequence.
ACCESSION   DATMFS010000491 DATMFS010000000
VERSION     DATMFS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17273"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VNQ33_09270"
     CDS             <1..720
                     /locus_tag="VNQ33_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98338.1"
                     /translation="WAIVRDTIAERPGTVVALPWLEYPSLSFADGRQAFNPLPDYLGG
                     DVISSYDPLFQASGVHQEQVDRRSVAVDELVDRARAGDPVAADLAALGVRWVVLAHER
                     GSERFGVLAADPGLDRILALPDADLYEVLGWDGAAVAPDGTAHRLSRPVPPILRTDAP
                     EGSVLNVAGAPGWVQGWGHPVAVTGDGRLRLEGTGETLWFWPSIVIFVTDVAIAGLGI
                     RCLASRRRRFGRILAPVGPRG"
     gene            793..1311
                     /locus_tag="VNQ33_09275"
     CDS             793..1311
                     /locus_tag="VNQ33_09275"
                     /inference="COORDINATES: protein motif:HMM:NF037006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWJ98339.1"
                     /translation="MKAAVLAGAIVALGLTFAGASPAGAQDCGYDCPPGSTSTTTTAV
                     PEIPTVTVVRGETIDVRGEGCAPGAEVVVTWDDGTVLGTFTADENGDFVTSITIPINA
                     TLGVHLVTATCGDVEQFLNVNVLAESVNNVDNGTLPRTGSSNTGPLVGIGVAAVVLGG
                     AFLYGARRPRQA"
     gene            1435..>2158
                     /locus_tag="VNQ33_09280"
     CDS             1435..>2158
                     /locus_tag="VNQ33_09280"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWJ98340.1"
                     /translation="MLFAHLLASGPWKPEFWLEKAGASAAWVIAAIIFAESGLFFGFF
                     LPGDSLLFLAGFLTSAGAEEFVQTTAGAPLATVVDHMPSIWVLLPLFFVMAVLGDQVG
                     YWFGKGVGPALFTREDSRLFKQSHVTKAHAFLEKYGPKTIFLARFVPIVRTFAPIVAG
                     VGQMRYRTFLKWNILGAFAWAVGVTLLGHVLGNVEVVREHIEIAILGIVAISLTPVFI
                     EVYRSRKHGRGDTPDGPVPGGPE"
BASE COUNT          269 a          771 c          744 g          374 t
ORIGIN      
        1 tgggcgatcg tccgcgacac catcgccgag cggccgggga cggtggtcgc cctgccgtgg
       61 ctcgagtacc cgtcgctgtc gttcgccgac ggccgccagg cgttcaaccc gctgcccgac
      121 tacctgggcg gcgacgtgat cagcagctac gacccgctct tccaggccag cggcgtgcac
      181 caggagcagg tcgaccggcg ctcggtggcc gtcgacgagc tggtcgaccg ggcccgagct
      241 ggtgatcccg tcgccgcgga cctggccgcg ctgggggtgc gctgggtcgt gctggcccac
      301 gagcgggggt cggagaggtt cggcgtgttg gccgccgatc ccggtctgga ccggatcctg
      361 gcgctgcctg acgccgatct gtacgaggtg ctgggttggg acggggcggc cgtcgccccc
      421 gacggcacgg cccaccgcct cagccggcct gtgccgccga tcctgcgcac cgacgccccg
      481 gagggctcgg tcctgaacgt ggccggcgct ccgggctggg tgcagggctg gggtcacccg
      541 gtggcggtga ccggcgacgg ccggctgcgg ctggagggga ccggcgaaac gctctggttc
      601 tggccctcga tcgtgatctt cgtcaccgac gtcgccatcg ctggcctggg aattcgttgc
      661 ctcgcctcac gtcgccgccg ttttggccga atacttgccc cagttgggcc gcgcgggtag
      721 cttcgtggtc accgttgcgg gaatcccctg cgtgtgaagg gtggaaggta cagacccatg
      781 attcgatcca agatgaaggc agcagtgctt gcgggtgcga tcgtcgccct gggcctcacg
      841 ttcgctggtg ccagcccggc tggtgcgcag gactgcggct acgactgccc tcccggctcg
      901 accagcacca ccacgacggc ggtcccggag atcccgacgg tcaccgtcgt gcgcggcgag
      961 accatcgacg tccgcggcga gggctgtgcg cccggcgccg aggtcgtcgt gacctgggat
     1021 gacggcaccg tgctcggcac cttcacggcc gacgagaacg gcgacttcgt cacctcgatc
     1081 accatcccga tcaacgccac cctcggcgtg cacctcgtca ccgccacgtg cggcgacgtc
     1141 gagcagttcc tcaacgtcaa cgtgctggcc gagtcggtca acaacgtcga caacggcacc
     1201 ctgccccgca ccggtagctc caacaccggc ccgctggtcg gcatcggtgt tgctgccgtg
     1261 gtcctcggtg gcgccttcct ctacggcgct cgccggcccc gtcaggcctg agccttccgc
     1321 cggcgctgag ccggcacacc cacccttcga gaacgacccc gcccctcggg cggggtcgtt
     1381 ccgcgttctg gcgcggtctt cgggccgatg ggggccggcc actagggtgc gaggatgctc
     1441 ttcgcccacc tcctggccag cggcccttgg aagccggagt tctggctcga gaaggcgggg
     1501 gccagcgccg cctgggtcat cgccgcgatc atcttcgccg agtccggctt gttcttcggc
     1561 ttcttcctgc cgggcgactc gctgctgttc ctggctgggt tcttgacgtc ggccggcgcc
     1621 gaggagttcg tgcagaccac ggctggggcc ccgctcgcca ccgtcgtgga ccacatgccg
     1681 tcgatctggg tgctgctgcc gctgttcttc gtcatggccg tcttgggcga ccaggtcggc
     1741 tactggttcg gcaagggcgt cggtccggcc ctgttcaccc gggaggactc gcgcctcttc
     1801 aagcagagcc acgtgaccaa ggcccacgcc ttcttggaga agtacggccc caagaccatc
     1861 ttcttggccc gcttcgtgcc gatcgtgcgc accttcgccc cgatcgtggc gggcgtcggc
     1921 cagatgcgct accgcacgtt cctcaagtgg aacatcctgg gggcgttcgc ctgggccgtc
     1981 ggcgtgaccc tgctcggcca tgtgctgggc aacgtcgagg tcgtccgcga gcacatcgag
     2041 atcgccatct tgggcatcgt cgccatctcg ctgaccccgg tcttcatcga ggtgtaccgg
     2101 agccgcaagc acggccgtgg cgacacgccc gacggtcccg tacccggcgg tcccgagg
//
LOCUS       DATMFS010000492         5516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_87252, whole genome shotgun sequence.
ACCESSION   DATMFS010000492 DATMFS010000000
VERSION     DATMFS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87252"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..3723)
                     /locus_tag="VNQ33_09285"
     CDS             complement(61..3723)
                     /locus_tag="VNQ33_09285"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HWJ98341.1"
                     /translation="MGIRQHSKRARIGAAAVALLIAIPVFNLVTGDSAKAASAVAGDG
                     TTSATAGASCWGIKQQIPASPNGLYWLNTAALERPQQFYCDMTTSGGGWVLVGRGREG
                     WTFNPFGQGSPSTVRTTVEGSGAFTPAALDTATISSLLNGQTVDTLADGIRLERATNT
                     AGTAKQNFAIYPKYKNWTWSFAAGQLLNKVVIDGTTYQGSNTYDTAASVSGQTTNGLS
                     GQSGVRRMFTYLWSSHNNKAGFSFGSGVNGGSNSATNHLWTNGSEGSPIPFTRVWIRP
                     QIANSAAGFSPIPAAGYGAATKPANLKNRSEQANWGVVGLNHANEPTIEPYSTNVLAL
                     KVFGNRTFVSGRFTGVQQGPTGTPVPQQSLAAFSIDGNFIDTFRPKIDGRVWDLALTA
                     DNKLIIAGDFTSVNGKPNTQGMAALDPDTGEVITSWKAKAYRADGTRSFVRALDVHDG
                     TVYAAGNFNRVDGGTWNTITVTNAVAVSAANGNPGLWRPRPNGSVVRLEATNDGSRVL
                     MAGYFTNINGDTNQGYFGITDAATGNVSTGIGAWQASAGSTAKYQQAVEDLGDGRILV
                     GGSEHDFQLWNANRTKMLDSTITKQGGDTQAIEVFDGKIYMGCHCGDWVYEGTNNWST
                     PSGFRSVQPINLVGQWDASTFTYDTSWYPSSLKGAKGEGIWAIDKDSRGCLWVGGDLN
                     RGAWSGNAATDYLGGFARFCPDDVTPPTTPANLTTTTAGSGVKLDWSASTDASGTVSY
                     DVYRDDRVIATVYGRSYTDPEVTGQHRYTVRAADARGNRSASPAPISVNGPSPVIATP
                     VPFGSTWKYNTTGVETAGWAANGFNDSAWASGPGKLGWGITGLGTTTTSTKPMTSYYR
                     KTFAVTNPSSVKQLDLKLAEFQGAVVHVNGVEVGRLNMPAGKITATTGAASYLTADGE
                     KAVKTITVPGSLLVSGTNTISVEVHSVTSGAGRQLFDLEATLYGAGGDTTPPSAPSLS
                     GTGGATANTLTWTGSTDDVALGGYIVRRDNQVIAVTGPTALGFTDGSITSPQSHSYSV
                     TAFDMNGNTAASNAVTLDPVADPNLLGFGANWKWFYAEGGPAADWAQPTFDDRTWATG
                     QAELGFGDSDEKTLISTNPAPRPLTGYFRTTVDIADPAAFRTVLASLIRDDSAVVYVN
                     GVEVGRDNLPAGPIAFATPASTIISDRAVEKTPISITIPASAFKAGTNVIAVEIHNND
                     RWSGDMSMDLKLTGQP"
     gene            complement(3871..4836)
                     /locus_tag="VNQ33_09290"
     CDS             complement(3871..4836)
                     /locus_tag="VNQ33_09290"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWJ98342.1"
                     /translation="MTTSTSTTSIGGRAGGGRTIDTTDASTLAKVRRSRAGTAVTDAA
                     KHALRAYGTATASHRKPPELIVIGAKRGGTTSLWRYLAEHPGVLPTFPKAEKIKGTYF
                     FDEEWHRGERWYRSHFPTDRVRAAAARTLGYEPIAFEASPYYLFHPHAPARARQVVPD
                     ALVVALLRDPVERAFSHYKERRNHTEPLPFAEALAAEEGRTAGEEDRMLADPSVVSFA
                     HRHQTYVAQGRYAPMLERWFAAFPSDQLLVAAAEDFYADPQAFCDQITDRVGLPRRDL
                     GSPEPFNSEPSADMDPEVRAQLRASLDDDIAAVEDLLGRPMPWGG"
     gene            complement(4833..>5516)
                     /locus_tag="VNQ33_09295"
     CDS             complement(4833..>5516)
                     /locus_tag="VNQ33_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWJ98343.1"
                     /translation="TGTNGWGAAVGILTPFFLRSWIVQPDQRRRRIGYAIALVGVYPI
                     LVSVNRGLWISLAVGMVYFAARKALRGKFAAFGVLCGAMVAVTVLLLATPAGQLVGDK
                     LDKSERSNDSRSTLYVEAFHGAVDSPLIGNGGPVRPPDAPPGMPPIGTHGMLWYLMFV
                     HGFVGMALFLAWLGGEVLRSGRVRTADGWWTHLALVIGLVQVPFYGLLPQVVLFGIAA
                     GLSHREDPT"
BASE COUNT          730 a         1714 c         2142 g          930 t
ORIGIN      
        1 gttctcaacg tggcatccat ctggaactca tcggcccgtg ggccggtgag ttcaggagaa
       61 tcagggctgg ccggtgagct tgaggtccat ggacatgtcc ccgctccagc ggtcgttgtt
      121 gtggatctcg accgcgatca cgttggtgcc ggccttgaag gccgaggccg ggatggtgat
      181 cgagatgggg gtcttctcga cggcgcggtc cgagatgatg gtcgacgctg gggtcgcgaa
      241 ggcgatcggg ccggccggca ggttgtcccg tccgacctcg acgccgttga cgtatacgac
      301 ggcgctgtcg tcacggatca ggctggccag cacggtccgg aacgcggccg gatcggcgat
      361 gtcgacggtg gtccggaagt agccggtcag ggggcggggg gcggggttcg tcgagatgag
      421 ggtcttctcg tcgctgtcgc cgaagccgag ctcggcttgg ccggtggccc aggtcctgtc
      481 gtcgaacgtg ggctgggccc agtcggccgc cgggccgcct tcggcgtaga accacttcca
      541 gttggccccg aacccgagca ggttggggtc ggccaccgga tcgagcgtga cggcgttcga
      601 cgcggccgtg ttgccgttca tgtcgaacgc cgtgaccgag tagctgtggc tctgggggct
      661 ggtgatgctg ccgtcggtga acccgagcgc cgtcgggccg gtgacggcga tgacctggtt
      721 gtcacggcgg acgatgtagc cgccgagggc gacgtcgtcg gtggagccgg tccacgtgag
      781 ggtgttggcc gtggctcctc cggtgccgct cagcgacggg gcggacggcg gtgtcgtgtc
      841 gccgccggcg ccgtagaggg tcgcctcgag gtcgaagagc tggcgcccgg caccgctcgt
      901 caccgagtgc acctccacgc tgatcgtgtt ggtgcccgac accaggagcg aacccgggac
      961 ggtgatcgtc ttgacggcct tctcgccgtc ggcggtcagg tagctggcgg caccggtggt
     1021 ggcggtgatc ttgccggcgg gcatgttgag gcggccgacc tcgaccccgt tgacgtgcac
     1081 caccgcgccc tggaactcgg cgagcttgag gtcgagctgc ttgaccgacg acgggttcgt
     1141 gaccgcgaac gtcttgcggt agtagctggt catcggcttg gtggaggtgg tggtggtgcc
     1201 caggccggtg atgccccagc cgagcttgcc gggacccgag gcccacgccg agtcgttgaa
     1261 gccgttggcg gcccaaccgg cggtctcgac gccggtggtg ttgtacttcc aggtgctgcc
     1321 gaagggcacc ggcgtggcga tgaccggcga cgggccgttg acgctgatgg gcgccggaga
     1381 tgcggagcgg ttgccgcggg cgtcggcggc gcggaccgtg tagcggtgct ggccggtgac
     1441 ctccgggtcg gtgtaggagc ggccgtagac cgtggcgatg acccggtcat cgcggtacac
     1501 gtcgtaggag acggtgccgc tggcatcggt ggaggcgctc cagtcgagct tcacgccgga
     1561 tccggcggtg gtcgtggtca ggttggccgg ggtcgtcggg ggggtgacgt cgtcggggca
     1621 gaaccgggcg aagccgccca ggtagtcggt ggcggcgttg ccgctccagg cgccgcggtt
     1681 gaggtcgccg ccgacccaga ggcagccccg gctgtccttg tcgatggccc agatgccttc
     1741 gcccttggcg cccttgaggc tcgacgggta ccacgacgtg tcgtaggtga acgtcgatgc
     1801 gtcccactgg ccgaccaggt tgatcggctg cacggagcgg aagccgctgg gggtgctcca
     1861 gttgttggtg ccctcgtaga cccaatcgcc gcagtggcag cccatgtaga tcttgccgtc
     1921 gaacacctcg atggcctgcg tgtcgccgcc ctgcttggtg atcgtggagt cgagcatctt
     1981 ggtgcggttc gcgttccaca gctggaagtc gtgctcggag ccgcccacca ggatgcggcc
     2041 gtcgccgagg tcctccaccg cctgctggta cttggcggtg gagccggccg atgcctgcca
     2101 ggcgccgata ccggtggaca cgttgccggt ggcggcgtcg gtgatgccga agtagccctg
     2161 gttcgtgtcg ccgttgatgt tggtgaagta gccggccatc aggacccggc tgccgtcgtt
     2221 cgtcgcctcg aggcggacga ccgagccgtt gggtcggggg cgccagaggc cggggttgcc
     2281 gttggccgcc gacacggcga cggcgttggt gacggtgatg gtgttccagg tgccgccgtc
     2341 gacccggttg aagttccccg cggcgtagac ggtgccgtcg tgcacgtcga gggcccggac
     2401 gaacgagcgg gtgccgtcgg cgcggtaggc cttggccttc cacgaggtga tgacctcgcc
     2461 cgtgtcgggg tcgagggcgg ccatgccctg ggtgttgggc ttgccgttca cgctggtgaa
     2521 gtcgccggcg atgatcagct tgttgtcggc cgtgagggcc aggtcccaga cccggccgtc
     2581 gatcttgggc cggaaggtgt cgatgaagtt gccgtcgatg ctgaaggcgg cgagcgactg
     2641 ctgggggacc ggggtgccgg tggggccctg ctggacgccg gtgaagcgac cgctgacgaa
     2701 cgtgcggttg ccgaacacct tcagggcgag cacgttcgtg gagtacggct cgatggtggg
     2761 ctcgttggcg tggttcaggc cgacgacgcc ccagttcgcc tgttcggacc ggttcttcag
     2821 gttggctggc ttcgtggcgg cgccgtagcc ggcggcgggg atgggggaga agccggcggc
     2881 gctgttggcg atctgggggc ggatccagac ccgggtgaac gggatcgggc tgccctcgct
     2941 gccgttcgtc cagaggtggt tggtggcgct gttcgagccg ccgttgacgc cggacccgaa
     3001 ggagaagccg gccttgttgt tgtggctgga ccacaggtag gtgaacatcc gccggacgcc
     3061 gctttggccg ctcaggccgt tggtggtctg cccggagacg ctggctgccg tgtcgtaggt
     3121 gttcgacccc tggtaggtgg tgccgtcgat gaccaccttg ttgagcagct ggcccgcagc
     3181 gaagctccag gtccagttct tgtacttggg gtagatcgcg aagttctgct tggcggtgcc
     3241 ggcggtgttg gtggcgcgct cgaggcggat gccgtcggcc agcgtgtcga cggtctggcc
     3301 gttgaggagc gagctgatgg tcgccgtgtc gagggcggcg ggcgtgaagg cgccggaccc
     3361 ctcgacggtg gtgcgcacgg tggacgggct gccctggccg aacgggttga acgtccaccc
     3421 ctcgcggccg cggccgacga gcacccagcc gccgccgctg gtggtcatgt cgcagtagaa
     3481 ctgctgcggc cgctcgaggg ccgcggtgtt gagccagtag aggccgttcg ggctggccgg
     3541 gatctgctgc ttgatgcccc agcaggatgc accggcggtg gcggaggtgg tcccgtcgcc
     3601 ggctaccgcg ctggccgcct tggccgagtc gccggtgacc aggttgaaca cggggatggc
     3661 gatgagcagc gcgaccgcgg ctgccccgat ccgtgctcgc ttgctgtgtt ggcggatgcc
     3721 catgtcgtgc cctcttcctc cccgctcccg cctgacaccg agcgtgccga gacgggaccg
     3781 aatatcacgc ttcgtgagag cgtacgtgct ttcacggagc gtgacaagct gctctgtgtc
     3841 accacgctct gtggttgatt ggcggcggcc tcaaccaccc cacggcatcg ggcggccgag
     3901 gaggtcctcg accgcggcga tgtcgtcgtc cagcgaggcc cggagctggg ctcgaacctc
     3961 gggatccata tcggcactcg gttcgctgtt gaaaggctcc ggcgagccga ggtcgcgccg
     4021 aggcaagccc acccggtcgg tgatctggtc gcagaacgcc tgcgggtcgg cgtagaagtc
     4081 ctccgccgcg gcgaccagga gctggtcgct cgggaaggcg gcgaaccagc gctcgagcat
     4141 gggggcgtac cggccctggg cgacgtacgt ctggtggcgg tgggcgaacg agaccaccga
     4201 ggggtcggcc agcatccggt cctcctcgcc ggccgtgcgc ccctcctcgg ccgcgagggc
     4261 ctcggcgaac ggcagcggct cggtgtggtt ccggcgctcc ttgtagtgcg agaacgcccg
     4321 ctccaccgga tcgcggagca gggcgacgac gagggcgtcc ggcaccacct ggcgggcccg
     4381 ggccggcgcg tgggggtgga acaggtagta ggggctggct tcgaaggcga tgggttcgta
     4441 gccgagggtc cgcgccgccg cggcccgcac gcgatcggtc gggaagtgcg agcggtacca
     4501 gcgctcgccg cggtgccact cctcgtcgaa gaagtaggtg cccttgatct tctcggcctt
     4561 cgggaaggtc ggcagcacgc cgggatgctc ggccaggtac ctccagaggg aggtggtccc
     4621 gccccgcttg gccccgatca cgatgagctc gggcggcttg cggtgcgagg cggtggcggt
     4681 gccgtaggcg cgcagcgcgt gcttggcggc gtcggtgacc gccgtcccgg cccggctgcg
     4741 gcgcaccttg gcgagcgtcg acgcgtcggt ggtgtcgatg gtccggccgc ccccggcgcg
     4801 gccgccgatg gaggtggtgg aggtcgacgt ggtcatgtgg ggtcctctcg gtgcgacagg
     4861 ccggcggcga tgccgaacag gacgacctgc gggagcaagc cgtagaacgg cacctggacc
     4921 aggccgatga ccagggccag gtgcgtccac cacccatcgg ccgttctcac ccgtcctgaa
     4981 cgcagcacct cgccgccgag ccaggcgagg aacagggcca tccccacgaa gccgtgcacg
     5041 aacatgaggt accagagcat cccgtgggtc ccgatgggcg gcatgcccgg tggggcgtcg
     5101 gggggccgga ccgggccgcc gttgccgatg agcggggagt cgaccgcacc gtggaacgct
     5161 tcgacgtaga gggtggaccg ggagtcgttg gaccgctccg acttgtcgag cttgtcgccc
     5221 acgagctgtc cggccggcgt cgccaggagc agcaccgtca ccgcgaccat cgccccgcac
     5281 agcacgccga acgcggcgaa cttgccccgc agcgccttgc gggcggcgaa gtagaccatg
     5341 ccgacggcca ggctgatcca caggccgcgg ttcaccgaca cgaggatcgg gtagacgccc
     5401 acgagcgcga tggcgtagcc gatgcgccgt cggcgctggt cgggttggac gatccaggac
     5461 cgcaggaaga acggggtcaa gatcccgaca gccgcacccc agccgttggt gccggt
//
LOCUS       DATMFS010000493         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_320479, whole genome shotgun sequence.
ACCESSION   DATMFS010000493 DATMFS010000000
VERSION     DATMFS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320479"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VNQ33_09300"
     CDS             complement(<1..1223)
                     /locus_tag="VNQ33_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ98344.1"
                     /translation="MTLPTAGTAPAPSPRSLLRGVFRAGPASRTLHLTWFAFFTTFVV
                     WFNFAPFATTIGDQFDLSKGQLVTLGLGNLALTVPARLVVGSLLDRFGPRRTFSAILV
                     YALVPCLVFATAQDFTGLVIGRLLVGVVGAGFVVGIRMVSEWFPPGAVGTAEGIYGGW
                     GNFGAAAATLALPVAADVVGGPDGWRWAIAGTGVAAAAFGLVYARSVTDTPGGVAYQR
                     TTKASALEVTSPSAVWGLMALSVPLAVALGVIGWRIWKVGVITTAGLAVAIAASALLL
                     AVQLLTVHRANGRARADGYPPEEQYPFRSVAVLSFAYACTFGSELAAVSFLPTFFETT
                     WGLSAAVAGAAASAFAVMNLVSRPAGGLISDLVASRRRWLATLLGCLGAGYLVLSRLT
                     GAWPLGVAIALVLGTS"
     gene            complement(1358..>2042)
                     /locus_tag="VNQ33_09305"
     CDS             complement(1358..>2042)
                     /locus_tag="VNQ33_09305"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWJ98345.1"
                     /translation="VQLDGDGSDGLAERLAELGLDVVPVPVYRWLLPDDLAPAERVVL
                     AVADGRVDAVSFTSAHAVANFAVIAASLGVLDRVRAACGPGRVAVVCVGPVTAARAQQ
                     LGLDGRVEPEQPRLGAMVARLAAAFEGRALDLSLDDVPVRLQGNLVRVGDRAPVSLTE
                     RERSVLEVLSERQGAVISKQALLQRVWHDDSDEHAVEVTVGRLRRRLGPAGGSIETVM
                     RRGYRLAAG"
BASE COUNT          320 a          738 c          783 g          201 t
ORIGIN      
        1 gaggtgccga ggaccagggc gatcgcgacg ccgagcggcc acgccccggt cagccgcgac
       61 aagacgaggt aaccggcccc caggcacccg agcaaggtgg cgagccagcg tcgccggctg
      121 gcgacgaggt cggagatgag cccgcctgct ggccgactga ccaggttcat caccgcgaac
      181 gccgacgccg ccgcgccggc gaccgcggcg gacaggcccc acgtggtctc gaagaaggtc
      241 ggcaggaacg acaccgccgc cagctccgag ccgaaggtgc aggcgtaggc gaacgagagc
      301 acggcgaccg accggaacgg gtactgctcc tcgggcgggt agccgtcggc tcgggctcgg
      361 ccgttggccc ggtgcacggt caggagctgg acggcgagca ggagggccga cgcggcgatc
      421 gcgaccgcca gcccggccgt ggtgatcacg cccaccttcc agatccgcca gccgatcacg
      481 ccgagggcga cggcgagcgg gaccgacagg gccatgaggc cccagacggc gctcgggctc
      541 gtcacctcga gcgcgctcgc cttcgtcgtc cgctggtagg cgacgccacc gggggtgtcg
      601 gtgaccgatc gggcgtagac gagcccgaac gcggcggcgg cgacgccggt cccggcgatg
      661 gcccagcgcc agccgtcggg gccgcccacg acatcggcgg ccacgggcag cgccagggtc
      721 gcggccgcgg caccgaagtt gccccaaccg ccgtagatgc cctccgcggt gccgaccgcg
      781 ccgggcggga accactccga caccatccgg atgccgacca cgaaaccggc gccgacgacg
      841 ccgaccagca gccgcccgat gacgaggccc gtgaagtcct gcgccgtggc gaacaccagg
      901 cacgggacca gcgcgtagac gaggatcgcc gagaacgtcc ggcggggccc gaagcgatcg
      961 agcaggctcc ccaccaccag ccgggccggg acggtgagcg ccaggttgcc gaggccgagg
     1021 gtcacgagct ggcccttgct caggtcgaac tggtcgccga tggtcgtggc gaacggcgcg
     1081 aagttgaacc agacgacaaa ggtcgtgaag aaggcgaacc aggtgaggtg cagggtgcgg
     1141 gaggccggtc cggcccggaa caccccgcgc aggaggctgc gaggagacgg cgccggtgcg
     1201 gtgccagcgg tcgggagggt catggcgcca tcctccggga cggggttttc ccggctgtgt
     1261 gcccgggatt acgccatccg aacgccgggc gcacgggggt cgggcgcggc tgtgcggggc
     1321 cgttcagctt cctgactcgg agcgctggcc gccgaggtca acctgcggcg aggcggtacc
     1381 cgcgccgcat gaccgtctcg atcgacccgc ctgccggccc gaggcgtcgg cgcaggcggc
     1441 cgaccgtgac ctcgacggcg tgctcgtcgc tgtcgtcgtg ccagacccgc tggagcagcg
     1501 cctgcttgga gatcaccgcg ccctggcgct ccgacagcac ctccagcacc gagcgctccc
     1561 gctccgtcag cgacaccggg gcccggtcgc cgacgcggac caggttcccc tggagccgga
     1621 cggggacgtc gtcgagcgac aggtccaggg cgcgaccctc gaaggcggcg gccaggcgag
     1681 cgaccatcgc tccgagccgg ggctgctcgg gctcgacgcg gccgtcgagc ccgagctgct
     1741 gggcgcgggc ggcggtgacc gggccgacgc acacgacggc gacccgcccc ggaccgcagg
     1801 cggctcgcac ccggtcgagc acgccgagcg aggcggcgat cacggcgaag ttggcgaccg
     1861 cgtgggcgga ggtgaagctc accgcatcga cccggccgtc ggcgacggcc agcacgacgc
     1921 gctcggccgg ggccaggtcg tcgggcagga gccagcggta gaccggcacc ggcaccacgt
     1981 cgagcccgag ctcggccagg cgctcggcga gcccgtcgga accgtcgccg tcgagctgga
     2041 cg
//
LOCUS       DATMFS010000494         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_270687, whole genome shotgun sequence.
ACCESSION   DATMFS010000494 DATMFS010000000
VERSION     DATMFS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270687"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VNQ33_09310"
     CDS             complement(<1..487)
                     /locus_tag="VNQ33_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWJ98346.1"
                     /translation="MTQGEELLSIGDRIVALARPGEQVEVVVGRSSSTEVRVYEGDVE
                     QLASATSAGIGVRVISDHRQGFAYAGSLDEDVLAETLADARDNATFATPDEWLGLAEP
                     DGIAVPDLDLYRESLLKASTELKIDLAIELEKALKAEDPRITGVDSADYADGVSEGAV
                     VS"
     gene            complement(633..2021)
                     /locus_tag="VNQ33_09315"
     CDS             complement(633..2021)
                     /locus_tag="VNQ33_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWJ98347.1"
                     /translation="MIDEAVVSRVLGTALQTGGDFAEVFAEDKRSSSGRLDDGKIEEL
                     GSGRDRGAGIRVVVGDTTGFAHTADLSEPGLLAAAEAAAAAARGGGGGVRTVALTEQA
                     VDSQAVLTRPEEVAKATKVELLQRANEVARSKGDAIKQVSASYGDSRRRILVANSDGL
                     LARDDQTRTLFVVGVVASGDTGMQTGRRSIGHTMGFELFDRYQVEDLADEAAEQALTK
                     LQARPAPSGALPVVIGKGGGGVLFHEACGHGLEADHIQKKVSVFADRVGQLVASPLVT
                     IVDDGTMGNEWGTFAIDDEGTPAQRNVLIEDGVLTDYMWDQLRARKEGRKLSGNGRRQ
                     SYKHLPMVRMTNTILANGETDPDDIVADTPSGVYVAHLGGGQVNTATGDFVFGMTEAY
                     LIEDGRITEPLREGNLIGNGPKVLELIDAVGDDFEMGPPGTCGKDGQGVPVGDGVPTL
                     RVSSLTVGGTAA"
     gene            2095..2472
                     /locus_tag="VNQ33_09320"
     CDS             2095..2472
                     /locus_tag="VNQ33_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HWJ98348.1"
                     /translation="MPVHLGLTAKITLVVSPDDTAVAARSGDVPVLSTPRIVALCEEA
                     AITAVEGQLTEGETTVGMRIQLDHLAPSAIGSTVTASAKLEKVEGRRLKFSVSVSDPR
                     GLVAAGKITRVVVERDRFMDKCL"
     gene            2504..3205
                     /locus_tag="VNQ33_09325"
     CDS             2504..3205
                     /locus_tag="VNQ33_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWJ98349.1"
                     /translation="MSSKGIEVVGLDADDTLWHSEVYFERTQRRFDELVGRYVSSDVD
                     VHAALTAVERRNVVRYGYGIKGFTLSMIECAIEITDGAIGVEELGQVLAAGRAMLDHP
                     VDLLDGVDETITALADAGYRMVIVTKGDLHHQEMKVMASGLAGRVERIEIVAEKDVAT
                     YQRVVASMDVEPEQFAMVGNSVRSDVLPVLELGGHAVHIPYHITWEAEHADHDGTVPT
                     LTTIAELPAWLASQS"
BASE COUNT          456 a         1192 c         1141 g          514 t
ORIGIN      
        1 tggacaccac ggcgccttcg gacacgccat cggcgtagtc ggccgagtcg acgccggtga
       61 tgcgcgggtc ctcggccttg agggccttct cgagctcgat ggccaggtcg atcttcaact
      121 cggtcgacgc cttcagcagg ctctcccggt agaggtcgag gtccggcacc gcgatgccgt
      181 cgggctcggc caggcccagc cactcgtcgg gcgtggcgaa cgtggcgttg tcgcgggcgt
      241 cggcgagcgt ctcggccagc acgtcctcat cgagcgaccc ggcgtaggcg aatccctgcc
      301 ggtggtccga gatgacccgg acgccgatgc ccgccgaggt ggctgatgcc agctgctcga
      361 cgtcgccctc gtacacccgc acctcggtgg acgacgaccg cccgaccacg acctcgacct
      421 gctcccccgg ccgagccagc gcgacgatcc ggtcaccgat cgacagcagc tcctctccct
      481 gggtcatcgc gcacctccgt cgcacaacgc cgatccgacg ccgctcacgg cccagccacg
      541 acgggcattt cgctgctccg caagctccgc atcggacgac caagccgtgt tcatgggtcg
      601 tactcgcttc gctcgccgtt ccgagacgtc gctcacgctg cggtccctcc cacggtcagg
      661 ctcgacaccc ggagggtggg gacgccgtcg ccgacgggga cgccctggcc gtctttgccg
      721 caggtgccgg gcgggcccat ctcgaagtcg tcgccgacgg cgtcgatgag ctcgagcacc
      781 ttcgggccgt tgccgatgag gttgccctcg cgcaggggct cggtgatcct cccgtcctcg
      841 atcaggtacg cctcggtcat gccgaacacg aagtctccgg tggccgtgtt gacctggccc
      901 ccgccgaggt gcgccacgta cacaccgctg ggggtgtcgg ccacgatgtc gtcggggtcg
      961 gtctcgccgt tggcgaggat cgtgttggtc atgcggacca tcggcaggtg cttgtagctc
     1021 tggcggcgcc cgttgcccga gagcttgcgg ccctccttgc gggctcggag ctggtcccac
     1081 atgtagtcgg tgaggacgcc gtcttcgatg agcacgttgc gctgggccgg cgtgccctcg
     1141 tcgtcgatgg cgaacgtgcc ccactcgttg cccatcgtcc cgtcgtccac gatggtcacc
     1201 agcggcgagg cgaccagctg gccgacccgg tcggcgaaca ccgacacctt cttctggatg
     1261 tggtcggcct cgagaccgtg gccgcaggcc tcgtggaaca gcacgccgcc gccgcccttg
     1321 ccgatgacga cgggcagggc acccgacggg gccgggcggg cctgcagctt ggtgagcgcc
     1381 tgctcggcgg cctcgtcggc caggtcctcg acctggtagc ggtcgaacag ctcgaagccc
     1441 atggtgtggc cgatcgagcg gcggccggtc tgcatgccgg tgtcgccgct ggccaccacg
     1501 cccacgacga acagcgtccg ggtctggtcg tcgcgggcga gcaggccgtc ggagttggcc
     1561 acgaggatgc ggcggcggct gtcgccgtag gacgccgaca cctgcttgat ggcatcgccc
     1621 ttcgaccggg cgacctcgtt cgcccgctgg agcagctcga ccttggtggc cttggccacc
     1681 tcctcgggac gggtgagcac cgcctgcgag tcgaccgcct gctcggtgag cgcgacggtc
     1741 cgcaccccgc cgcccccgcc tcgagcggcg gcggcggccg cttcggcggc ggcgaggagg
     1801 cccggctcgg acaggtcggc cgtgtgggcg aagccggtgg tgtcgcccac cacgacccgg
     1861 atgccggcac cgcggtcgcg gcccgagccg agctcctcga tcttgccgtc gtcgaggcgg
     1921 cccgacgacg agcgcttgtc ctcggcgaac acctctgcga agtccccgcc cgtctgcagg
     1981 gcggtgccga gcacccggga caccacggct tcgtcgatca cgtcggacct cgtccttgtc
     2041 gaatgttgag cgggcgcttg gatcgcccgt ccgagggcgg cttatcgtac cgaggtgcct
     2101 gtgcatctcg gactgaccgc gaagatcacg ttggtcgtct cccccgacga caccgcggtg
     2161 gccgcccggt ccggcgacgt ccccgtgctg tcgacgcccc gcatcgtcgc cctctgcgaa
     2221 gaggcggcca tcaccgcggt cgaaggccag ctcaccgagg gcgagaccac cgtcggcatg
     2281 cggatccagc tcgaccacct cgccccctcc gccatcggca gcacggtgac ggccagcgcc
     2341 aagctcgaga aggtcgaggg ccgccggctg aagttctcgg tctcggtcag cgacccccgc
     2401 ggcctcgtcg ccgccggcaa gatcacccgg gtggtcgtcg agcgagaccg cttcatggac
     2461 aagtgcctgt agcgatctcc tcgcggcctg gcatcctgcc tcggtgagct ccaaggggat
     2521 cgaggtcgtc gggctcgacg ccgacgacac gctgtggcac tcggaggtgt acttcgaacg
     2581 gacccagcgg cggttcgacg agctggtcgg gcgctacgtc agcagtgacg tcgacgtcca
     2641 tgccgcactg accgcggtcg agcggcgcaa cgtcgtccgc tacggctacg gcatcaaggg
     2701 cttcacgctc tcgatgatcg agtgcgccat cgagatcacc gacggcgcca tcggcgtcga
     2761 ggagctgggc caggtgctgg cggcgggccg ggccatgctc gaccaccccg tcgacctgct
     2821 cgacggcgtc gacgagacga tcaccgccct ggccgacgcc ggctaccgca tggtcatcgt
     2881 caccaagggc gacctgcacc accaggagat gaaggtgatg gcatcgggac tcgccggccg
     2941 ggtggagcgg atcgagatcg tggccgagaa ggacgtggcg acctaccagc gggtcgtggc
     3001 ctcgatggac gtggagcccg agcagttcgc catggtcggc aactcggtca ggagcgacgt
     3061 gctcccggtg ctcgagctcg gcggccacgc cgtccacatc ccgtaccaca tcacctggga
     3121 ggccgagcac gccgaccacg acggcaccgt ccccacgctc acgacgatcg ccgagctccc
     3181 ggcctggctc gccagccagt cctgaggtcg gcgggagatc agaccggcgg gagctcagat
     3241 cggggtggtg ctggcctcgg cgggggtgct ctccaccgcg gtctcggact cgctctcggt
     3301 gcc
//
LOCUS       DATMFS010000495         3031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_343401, whole genome shotgun sequence.
ACCESSION   DATMFS010000495 DATMFS010000000
VERSION     DATMFS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343401"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VNQ33_09330"
     CDS             complement(<1..911)
                     /locus_tag="VNQ33_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWJ98350.1"
                     /translation="MTSDVCGLDEVGSRDLAAVGGKGASLGELAGIDGVTVPAGFCVT
                     TGAFERALAAASVDRLLERLEPLAADDLGAVAALAAEIRTAIEAVEVPAAVADEIREA
                     VGRVGGEDTPWAVRSSATAEDLPTVSFAGQQDTFLNVVGPDAVIDHVRRCWASLFTDR
                     AVAYRQHHGFDHRGVRMAVVVQRMVEPTASGVLFTADPVSGNRNLATIDAGFGLGEAL
                     VSGLVNPDVFAVRDGEVVDRSIAVKTTATRAAAGGGTEQVAVDASRWEEAALTDAQAV
                     ELVALARRIEGRLGAPQDIEWCLVDDG"
     gene            complement(1068..1838)
                     /locus_tag="VNQ33_09335"
     CDS             complement(1068..1838)
                     /locus_tag="VNQ33_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98351.1"
                     /translation="MPPDRSRRIDRSQLPGEFTPETVRRLGMRTARALAHPDNGVLTP
                     EEQVAFDRVLREVMQESAERLGRSVGRSRRPPPPDADPHLRRSYLRTQQRLDAQAERA
                     RQAFPQLAGGWADPAPPARPVDGAPVGAAPVDPARPVGGVEAAADGAADDEVSVGAFE
                     HEIEQTSDTLEILEQIAGIQQQQLEHQRSQLLSETRGLFFALAVSVAVIIAGVAPLVS
                     ASPHDRRSILAWTAIVCAVGGVAYAIVRSVQAKAGRPD"
     gene            complement(1874..2431)
                     /locus_tag="VNQ33_09340"
     CDS             complement(1874..2431)
                     /locus_tag="VNQ33_09340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HWJ98352.1"
                     /translation="MDTGDEIRIPSGTAASRAFVADVERAMAITARLNRLTYADLDEV
                     RELFTELTREAPGEGFGLIPPFFADCGRAIRIGTHVFVNQNATFSSLAPITISDEVMI
                     GPNVSLITSGHPVAPSQRHAALVGRPITIERDVWIGAGATVIGGVTIGARSVVAAGSV
                     VTKDVPPDSLVGGNPATLIRSIAGE"
     gene            complement(2437..2784)
                     /locus_tag="VNQ33_09345"
     CDS             complement(2437..2784)
                     /locus_tag="VNQ33_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWJ98353.1"
                     /translation="MDHPAPDQANGLQNERTTLAWRRTNLAALTVSALAAKASGRPAV
                     ALAVFGGAFGVCALVGLLADQRGRQRAAAIDDWDAGRCAASSSAAAPLAVAAAAGLTV
                     VLAALGVAIALLP"
     gene            complement(2790..>3031)
                     /locus_tag="VNQ33_09350"
     CDS             complement(2790..>3031)
                     /locus_tag="VNQ33_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98354.1"
                     /translation="GGLGIDAFAHDLPVWARTSISIALVVVGGLLGASAFRRWLQAEV
                     ALRRDEPLPPSRTPQIVSLAIAAVALAVAILIAFE"
BASE COUNT          423 a         1140 c         1102 g          366 t
ORIGIN      
        1 aacccgtcgt ccacgagaca ccactcgatg tcctgcggcg caccgaggcg gccctcgatc
       61 cggcgcgcga gcgccacgag ctccaccgcc tgggcatcgg tcagcgccgc ctcctcccac
      121 cgcgacgcgt ccacggccac ctgctcggtc cccccacccg cggcggcacg ggtggccgtc
      181 gtcttgacgg cgatggagcg gtcgacgacc tcgccgtcgc gcacggcgaa cacgtcgggg
      241 ttgaccaacc cggacacgag cgcctcaccc aacccgaacc cggcgtcgat ggtggcgagg
      301 ttccggtttc ccgagacggg atcggcggtg aagaggactc ccgacgccgt cggctcgacc
      361 atccgctgca ccaccacggc catgcggacg ccacggtggt cgaacccgtg gtgctggcgg
      421 tacgccaccg cccggtcggt gaacagggag gcccagcagc ggcggacgtg gtcgatcaca
      481 gcgtcggggc cgaccacgtt gaggaacgtg tcctgctgtc cggcgaacga caccgtcggc
      541 aggtcttcgg cggtggcgct cgaccgcacc gcccacggcg tgtcctcgcc gccgacacgg
      601 ccgaccgcct ctcggatctc gtcggccacc gcggccggca cctcgaccgc ctcgatcgcc
      661 gtccggatct ccgccgcgag ggccgcgacc gcgccgaggt cgtccgcagc gagcggctcg
      721 agccgctcga gcaaccggtc gaccgacgcg gccgcgagcg cccgttcgaa cgcccctgtc
      781 gtgacgcaga acccagccgg cacggtcacg ccgtcgatgc cagccagctc gccgaggctg
      841 gcccccttgc cccccacggc cgccaggtcg cggctcccaa cctcgtcgag tccgcacacg
      901 tcgctcgtca tcggttcaca tcccttcggc ccggaagccg ttcgcgcctc gccgtcgggc
      961 gagatcaaga ggtgcagtgg aagcacgatt cgccgcttgc aacaagcccc ccaccctccg
     1021 gtaccttggg tactggcggg gcggacgggc ggtgggtgga ccgtcgatca gtcgggtcgg
     1081 ccggccttcg cctgcaccga tcggacgatg gcgtaggcca ccccaccgac cgcgcacacg
     1141 atcgccgtcc acgccaggat cgaccgccgg tcgtgcggcg atgccgacac cagcggggcg
     1201 acgccggcga tgatcacggc aaccgacaca gccagggcga agaagaggcc gcgggtctcg
     1261 ctcagcagct gcgagcgctg gtgctcgagc tgctgctgct ggatcccggc gatctgctcg
     1321 aggatctcca gcgtgtccga cgtctgttcg atctcgtgct cgaacgcgcc gaccgacacc
     1381 tcgtcgtcgg ccgcaccgtc ggccgcggcc tcgacgccgc ccaccggccg agcagggtcg
     1441 acgggggccg ccccgaccgg cgccccgtcg accggcctcg ccggcggtgc cgggtccgcc
     1501 cacccgcctg ccagctgcgg gaaggcctga cgggcccgct cggcctgggc atcgaggcgc
     1561 tgctgggtcc gcaggtagga ccggcgcagg tgcgggtcgg catcgggcgg cggcggccgg
     1621 cgcgaccgcc cgaccgatcg gccgaggcgc tcggcggact cctgcatcac ctcgcgcagg
     1681 acccggtcga acgccacctg ctcctcgggc gtcaagacgc cgttgtccgg gtgcgccagc
     1741 gcccgggccg tccgcatgcc gagccggcgc acggtctccg gcgtgaactc gccgggcagc
     1801 tgcgagcggt cgatgcgacg ggagcggtca ggcggcaccg ccccatcatg gcggcggacc
     1861 ggcccggccc gggctactcg ccggcgatcg agcggatcag cgtcgccggg ttgcccccca
     1921 ccagggagtc cggcggcacg tccttggtga ccaccgaccc ggcggcgacc accgagcggg
     1981 cgccgatcgt gacgccgccg atgacggtgg cgccggcacc gatccacacg tcgcgctcga
     2041 tggtgatcgg ccggccgacg agcgcggcgt ggcgctgcga cggagcgacc gggtggcccg
     2101 acgtgatcag gctcacgttg ggcccgatca tcacctcatc gctgatcgtg atcggggcga
     2161 gcgaggagaa ggtggcgttc tggttcacga acacgtgggt gccgatgcgg atggcgcgcc
     2221 cgcagtcggc gaagaacggc gggatcagcc cgaacccctc tcccggcgct tcacgggtga
     2281 gctcggtgaa cagctcccgg acctcgtcga ggtcggcgta ggtgaggcgg ttgagccgag
     2341 cggtgatcgc catcgcccgc tcgacgtcgg ccacgaacgc ccgcgacgcc gcggtgcccg
     2401 acgggatgcg gatctcgtcg ccggtgtcca tcggcatcag gggagcaggg cgatggccac
     2461 gccgagggca gccagcacga cggtcaggcc ggcggccgcg gccacggcga gcggtgccgc
     2521 cgccgatgag ctggccgcac accgaccggc gtcccagtcg tcgatcgcgg cggcccgctg
     2581 ccggccgcgc tggtcggcga gcagtccgac cagcgcgcac accccgaacg ccccgccgaa
     2641 cacggcgagc gccacggccg ggcggcccga tgccttggcg gcgagggccg agacggtgag
     2701 ggcggcgagg ttggtccgcc gccaggcgag cgtcgtgcgc tcgttctgga ggccgttcgc
     2761 ctggtcaggc gctgggtgat ccaccgtcgc tactcgaacg cgatgaggat ggcgacggcg
     2821 agggcgaccg cggcgatggc gagcgacacg atctgcggcg tgcggctggg cgggagcggc
     2881 tcgtcgcggc gcagggcgac ctccgcctgg agccaccgcc ggaacgccga cgcgcccagc
     2941 agtccgccga cgaccacgag cgcgatcgag atcgaggtcc tcgcccagac ggggaggtcg
     3001 tgggcgaacg cgtcgatgcc caggccgccg g
//
LOCUS       DATMFS010000496         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_177966, whole genome shotgun sequence.
ACCESSION   DATMFS010000496 DATMFS010000000
VERSION     DATMFS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177966"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VNQ33_09355"
     CDS             complement(<1..366)
                     /locus_tag="VNQ33_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWJ98355.1"
                     /translation="MRKTSVYLTDELKESLASAAAQSGRSEAEFIRAAIELAVQQAKT
                     GSATAPTPPVRRVGPALIGVGVGPGAADLLTPRAHDAIATADTVVAAAISADAIGRAE
                     SIARAASDPLRVLRLEIDIA"
     gene            420..1151
                     /gene="cobA"
                     /locus_tag="VNQ33_09360"
     CDS             420..1151
                     /gene="cobA"
                     /locus_tag="VNQ33_09360"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWJ98356.1"
                     /translation="MTVYLVGAGPGDPGLITVRGAQVLARAEVVVHDRLSAAELLDLA
                     PAAAERIDVGKAPKAHRMPQAEINQLLVQRGQAGQAVVRLKGGDPFVFARGSEEAQAL
                     AGAGVPFEVVPGITSAIAVPAYAGIPITQRFSSTSFTVVTGHEDPSSGDGTVDWDAVA
                     RIGGTVVILMGVGRWPQIAARLLAAGRPPDTPAAAVRWGTRPEQCTIRATLATLGDHP
                     LAPPSVIVVGSVAAETLEWTEHLGG"
     gene            complement(1215..2039)
                     /locus_tag="VNQ33_09365"
     CDS             complement(1215..2039)
                     /locus_tag="VNQ33_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HWJ98357.1"
                     /translation="MARNVAVAVACCLAVFGLGAAVGSRAGIGDSGPPTTTTTLSPDD
                     QAIVDGNLAKCNDLSYSDNASFGATCSSHGGVRKWLATYGRCADGEVIRISDTAKCGD
                     HGGFDKLLPAGFVPTAAKGDLAECNDGTFSDNTDFDATCSSHQGLDRWLAPYARCTDG
                     TVIKLARSASCDHHGGFGKLMAKDYTPPTTTTTAAATTTTAPPPPPPPTTTAPIQPLV
                     PPTTAPYVPPTDPPSTAPPVAAGGGHTARCNDGTYSDAAHHQGACSHHGGVAEWYK"
     gene            complement(2456..>2792)
                     /locus_tag="VNQ33_09370"
     CDS             complement(2456..>2792)
                     /locus_tag="VNQ33_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98358.1"
                     /translation="LLMGAAACGSSGGDDAKKSDTTEAKSDSGSDEGATTDSSNADVK
                     AYCDAVDEYVKKAKAAQSDPAKASALTDEAKDLTDKATALASAGLDADDAQAVADCTK
                     KSTDALMPS"
BASE COUNT          338 a          920 c         1110 g          424 t
ORIGIN      
        1 ggcgatgtcg atctcgagcc gcagcacccg gagcggatcg gaggcggccc gggcgatgga
       61 ctcggcccgg ccgatggcgt cggcgctgat ggcggccgcc acgaccgtgt cggccgtggc
      121 gatggcgtcg tgcgcccggg gcgtgaggag gtcggcggcg cccgggccca cgccgacgcc
      181 gatcagggca ggcccgactc ggcggacggg cggggtgggg gcggtggccg agccggtctt
      241 ggcctgctgc acggccagct cgatggccgc ccggatgaac tcggcctcgg agcgtccgga
      301 ctgtgcagcc gccgaggcca gcgattcctt gagctcgtcg gtcaggtaca ccgaggtctt
      361 ccgcatggca cgtagggtac tgcgtagggc agtcccgtct ccccggtacc atcgccagcg
      421 tgaccgtcta cctcgtgggt gcaggacctg gtgatcccgg gctgatcacg gtgcggggcg
      481 cgcaggtgct cgcccgggcc gaggtggtcg tccacgaccg gttgtcggcg gccgagctgc
      541 tcgacttggc cccggcggcg gccgagcgca tcgacgtcgg caaggcgccc aaggcccacc
      601 gcatgcccca ggccgagatc aaccagctcc tggtgcagcg cggccaggcc ggccaggcgg
      661 tcgtccggct caaaggcggc gacccgttcg tcttcgcccg cgggagcgag gaggcccagg
      721 ccctggccgg cgccggggtg ccgttcgagg tcgtcccggg catcacgtcg gcgatcgcgg
      781 tcccggccta tgccggcatc cccatcaccc agcgcttctc ctcgacctcg ttcacggtgg
      841 tgaccggtca cgaggacccg tcgagcggcg acgggaccgt cgactgggac gcggtggcgc
      901 gcatcggcgg gacggtcgtg atcttgatgg gcgtgggacg ctggccccag atcgcggcgc
      961 ggctgctggc tgcaggtcgg ccacccgaca ccccggcggc ggcggtccgc tgggggaccc
     1021 gcccggagca gtgcaccatc cgagccacgc tcgccacgct gggcgatcac ccgctggcgc
     1081 cgccatcggt gatcgtcgtc ggatcggtcg cagccgagac gctcgagtgg acggagcacc
     1141 tcggcgggta gcgagtggcc cgtcgatctc ggcgggccag cggggatgtc gcgtggagcc
     1201 cgtccgaacg agggctactt gtaccactcg gctacgccgc cgtggtggga acaggctccc
     1261 tggtggtgtg cagcatcgct gtaggtgccg tcgttgcatc gggcggtgtg gcccccgccg
     1321 gccgcgaccg gaggggccgt gctcggggga tcggtcggcg gcacgtaggg agcggtcgtg
     1381 ggcgggacga gaggttggat cggcgccgtg gtggtcggcg gtgggggagg tgggggagct
     1441 gtcgtggtgg tagcggcggc ggtggtcgtc gtggtcggcg gcgtgtagtc cttcgccatg
     1501 agctttccga agccgccgtg gtggtcgcag gacgcggatc gggccagctt gatgacggtg
     1561 ccgtcagtgc agcgggcgta cggggcgagc caccggtcga ggccctgatg gctgctgcag
     1621 gtcgcgtcga agtccgtgtt gtccgagaag gttccgtcgt tgcactcggc gaggtcgccc
     1681 ttggcggcgg tggggacgaa cccggcgggc aggagcttgt cgaacccgcc gtggtcgccg
     1741 cacttggccg tgtcggagat gcggatgacc tcgccgtcgg cgcaccggcc gtaggtggcc
     1801 agccacttcc ggaccccgcc gtggctgctg cacgtcgcgc cgaagctggc gttgtcggag
     1861 taggacaggt cgttgcactt ggcgaggttg ccgtccacga tcgcctggtc gtcggggctc
     1921 aacgtcgtgg tcgtcgtcgg gggcccgctg tcgccgatcc ctgcacggga gccgacggcg
     1981 gcaccgaggc cgaacacggc caggcagcag gcgaccgcga ccgcaacgtt gcgcgccacc
     2041 ttcgcccgtc gagccggacc ggccggcttc ggtggcggcg gcgggagcag cggcggaggc
     2101 ggcgccggtg gcagcggggc cgcgctcggc ccgctgaggg cgttgccgca cgacgtgcag
     2161 aagcgcgctg ctggaacgtt gacgcgtccg cacccggaac aggtctccat tgcatcccct
     2221 ccccaatgaa tcctggcagg gaggctaggg cgaccgacca gatccgatcg agtccgacac
     2281 cccggcccga tggccacgga caagcaagcg ggaactggtc gacggggcca gctacgaggt
     2341 gaacgaggcc gtggtgggcg aacgcagccc atggccgagc gcggatgtgc caacgccccc
     2401 gccgcgggag cggcgagggc gctggaggtc cgggggagga ccggggggat cagcgtcagc
     2461 tgggcatgag ggcgtcggtc gacttcttgg tgcagtcggc cacggcctgg gcgtcgtcgg
     2521 cgtcgaggcc ggcgctggcg agggccgtcg ccttgtcggt caggtccttg gcctcgtcgg
     2581 tcagggccga ggccttggcc gggtcgctct gggcggcctt ggccttcttg acgtactcgt
     2641 cgacggcgtc gcagtaggcc ttgacgtcgg cgttgctgct gtcggtcgtc gccccttcgt
     2701 cgctgccgct gtcgctcttg gcctcggtgg tgtcggactt cttggcgtcg tcgccgccgc
     2761 tgctgccaca ggccgctgcg cccatgagca ag
//
LOCUS       DATMFS010000497         2482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_87339, whole genome shotgun sequence.
ACCESSION   DATMFS010000497 DATMFS010000000
VERSION     DATMFS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87339"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VNQ33_09375"
     CDS             <1..1260
                     /locus_tag="VNQ33_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ98359.1"
                     /translation="LASSTRRQAYAQTLVDQRTVKLREALAEQERLQEGQRVARQAAE
                     QANQAKDEFMSRMSHELRTPLNAVLGFAQLLEQDDLDEDGRDSVVQILKGGRHLLDLI
                     NEVLDISRIESGTLQLSSEPVSVATVIADVVQLAAPLAAADGITLVAGPPPSDGTHVL
                     ADHQRLIQILLNLVGNAIKYNRPGGTVDVSSEPVAGSRMRIKVADTGPGIHPEQRALL
                     FKPFERLGAERTDIEGTGVGLALSRHLADAMGGTLDVDSVVGQGSTFWVDLPLVEGPE
                     ERFERMQEDTPPAQSEPPARDRAKVLSIEDNLLNVRLVTRIVEQRPGVELLTAMQGRL
                     GFEIAREHQPKLVLLDLHLSDMGGEEVLRMLRADPRTAHIPVTVVSADATPGQIRRLL
                     AEGATSYMTKPLDVRAMLDLLDEALGR"
     gene            1257..>2482
                     /locus_tag="VNQ33_09380"
     CDS             1257..>2482
                     /locus_tag="VNQ33_09380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HWJ98360.1"
                     /translation="MTDAGREDPRTAPDDPVMADAIVLVVDDNGPNCVLLERILTRAG
                     VAQVHTTDDPFQVVSMVERIKPHLVLLDLHMPGMDGVAVMEAVAGVTPPDEFLPVIVL
                     TADATSAARHRALEAGATDFLTKPIERIEVVLRVRNHLHTRSLHARLHARNEELVTEV
                     AEHRRMDDAARAALAAKERRVGQLIDSGPPAIAFQPIAELATGTVIGYEGLARFASEP
                     LRPPDHWFAEAAEVGRGVELELAAVQAALADAARYFPETPVSVNISPAAACSPDLPPL
                     LSRRDGRLLFLEITEHERVDHYEQLLAALGRLRATGARLAVDDAGAGFAGLQHILTLR
                     PDIIKLDIALTRGIDQDPIKRALASSLVTFSGEMNSRLVAEGIETEAELVTLIDLGVA
                     WGQGFHLGRPGPAETFA"
BASE COUNT          385 a          929 c          836 g          332 t
ORIGIN      
        1 ctcgccagca gcacccgccg gcaggcctac gcccagaccc tcgtcgacca gcgcacggtc
       61 aagctccgag aggcgctggc cgaacaggag cggctccagg agggccagcg ggtcgcccgg
      121 caggcggctg agcaggccaa ccaggccaaa gacgagttca tgtcgcgcat gagccacgag
      181 ctgcgaacgc cgctcaacgc cgtcttgggc ttcgcccagc tgctcgagca agacgacctc
      241 gacgaggacg gccgcgactc ggtcgtccag atcctcaagg gcggccgcca cctgctcgac
      301 ttgatcaacg aggtgctcga catctcccgc atcgagtccg gcaccctcca gctctcgtcc
      361 gagccggtgt cggtcgcgac ggtgatcgcc gacgtggtcc agctcgccgc cccgctggct
      421 gcggcggacg ggatcacctt ggtggccgga cctccgccga gcgacggcac ccacgtgctc
      481 gccgaccacc agcggctcat ccagatcctg ttgaacctcg tcggcaacgc catcaagtac
      541 aaccggccgg gcgggacggt cgacgtctca tcggagcccg tcgccgggtc tcggatgcgg
      601 atcaaggtgg ccgacacggg cccaggcatc caccccgagc agcgagcgct gctgttcaag
      661 ccgttcgagc ggctcggtgc cgaacgcacc gacatcgagg ggaccggtgt cggcctggcg
      721 ctctcgcgcc acctggccga tgccatgggc gggaccctcg acgtcgactc cgtggtgggg
      781 cagggcagca cgttctgggt cgacctcccc ctggtggagg gtcccgagga gcggttcgag
      841 cgcatgcagg aggacacacc ccccgcccag agcgagccac cggccaggga ccgcgccaag
      901 gtgctctcca tcgaggacaa cctgctgaac gtgcggctgg tgacccgcat cgtggagcag
      961 aggccaggtg tggagctcct caccgcgatg cagggccggc tcgggttcga gatcgctcgg
     1021 gagcaccagc ccaagctcgt gctgctcgac ctccacctct cggacatggg cggcgaggag
     1081 gtgctccgca tgctccgggc cgatccacgc accgcccaca tccccgtcac cgtcgtcagc
     1141 gccgacgcga ccccgggcca gatccgtcgg ctcctcgccg agggcgccac ctcctacatg
     1201 acgaagccgc tcgatgtccg agcgatgctc gacctgctcg acgaggcgct gggtcgatga
     1261 ccgacgcggg ccgcgaggat ccgaggacgg cgccagacga cccggtgatg gccgacgcga
     1321 tcgtgctcgt ggtcgatgac aacggcccga actgcgtgct cctcgagcgg atcctcacgc
     1381 gagccggcgt cgcccaggtg cacaccaccg acgacccgtt ccaggtcgtc tccatggtcg
     1441 agcggatcaa gccccacctc gtcctgctcg acctccacat gccggggatg gacggggtgg
     1501 cggtcatgga ggcggtcgcc ggcgtgacac cgcccgacga gttccttccg gtgatcgtgt
     1561 tgacggcaga cgccaccagc gccgcccgcc accgggcgct ggaggcgggg gcgaccgact
     1621 tcctcacgaa gccgatcgag cggatcgagg tggtgctccg ggtcaggaac cacctccaca
     1681 cccggtccct ccacgcccgg ctccacgccc ggaacgagga gctggtgacc gaggtcgccg
     1741 agcaccgccg gatggacgac gccgcccgcg ccgcgctggc ggcgaaggag cggagggtgg
     1801 gccagctcat cgacagcggc ccgcccgcca tcgccttcca gccgatcgcc gagctggcca
     1861 ccggaacggt gatcggctac gaggggctgg cccgcttcgc gagcgagccc ctccgtccac
     1921 cggaccactg gttcgccgag gcggcagagg tcggccgagg ggtcgagctc gagctcgccg
     1981 cggtgcaggc ggcactggcg gatgcggcgc ggtacttccc ggagacgccc gtgtcggtca
     2041 acatctcccc cgcggctgcc tgcagccccg acctcccccc gctgctctcc cggcgagatg
     2101 gccggctgct cttcctcgag atcaccgagc acgagcgcgt cgaccactac gagcagctgc
     2161 tcgccgcgct ggggcgcctg cgggccaccg gtgcgcgcct cgccgtcgat gacgccggcg
     2221 ctgggttcgc gggcctccag cacatcctca cgctgcgccc cgacatcatc aagctcgaca
     2281 tcgcgttgac ccgaggcatc gaccaggacc cgatcaagcg ggcgctcgca tcttcgctcg
     2341 tcaccttcag cggcgagatg aacagccgcc tcgtcgccga gggcatcgag accgaggccg
     2401 agctcgtcac gctgatcgac ctgggcgtcg cctggggcca gggcttccac ctcggccgcc
     2461 cggggcccgc cgagacgttc gc
//
LOCUS       DATMFS010000498         4747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_62871, whole genome shotgun sequence.
ACCESSION   DATMFS010000498 DATMFS010000000
VERSION     DATMFS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62871"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNQ33_09385"
     CDS             complement(<1..228)
                     /locus_tag="VNQ33_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWJ98361.1"
                     /translation="MRLVHPPIHPSSASFDELVAAAKAGDAGSFEELVRRTSADTYTL
                     ARRLVSDDDDARDVVQDAYLRAYRSIGTFRGD"
     gene            421..493
                     /locus_tag="VNQ33_09390"
     tRNA            421..493
                     /locus_tag="VNQ33_09390"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Trp,seq:cca)
     gene            533..835
                     /gene="secE"
                     /locus_tag="VNQ33_09395"
     CDS             533..835
                     /gene="secE"
                     /locus_tag="VNQ33_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWJ98362.1"
                     /translation="MAMNREHKRMLQKQGQLDAEGNQKAERRKAPSPTAPKEPRTKPR
                     QFVREVNAELRKVAWPSRNETISLSAIVLVFLIIMTALIAALDYSFSEAVLWIINQ"
     gene            1047..1640
                     /gene="nusG"
                     /locus_tag="VNQ33_09400"
     CDS             1047..1640
                     /gene="nusG"
                     /locus_tag="VNQ33_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWJ98363.1"
                     /translation="MAEPAPVVDNPANRPGKWYVVHTQSGYEKKVKQNLEARTSSMNM
                     EGRINEVVIPMEEVTEFKNGKKVIVQKKMFPGYLLVRCFLDDDSWYVIRNTPGVTGFV
                     GQGTKPSPLRRKEVETFLSIPVEGDTSSPSVGARPARPRLEWEISETVRVKEGPFADF
                     SGEIVEIDEDKLKVKVLVNIFGRETPVELDFSQIAKL"
     gene            1824..2249
                     /gene="rplK"
                     /locus_tag="VNQ33_09405"
     CDS             1824..2249
                     /gene="rplK"
                     /locus_tag="VNQ33_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWJ98364.1"
                     /translation="MAKKKVKAVVKIQIPAGQATPAPPVGTALGPHGVAIMDFCKAYN
                     AQTEASRGQIIPVEITIFEDRSFTFITKTPPASRMLLEAAGVAKASSNPGKESGGSVT
                     AAQIEEIAKVKLPDLNANDLDAAKKIVEGTARSMGLAVN"
     gene            2360..3061
                     /gene="rplA"
                     /locus_tag="VNQ33_09410"
     CDS             2360..3061
                     /gene="rplA"
                     /locus_tag="VNQ33_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWJ98365.1"
                     /translation="MAQGKKYSDAIKRYDTEALHGIGEALGHVKALAPAQFDETVELV
                     VRLGVDPRKADQMVRGTVALPAGTGKDVRIAVFATGDAAAAAREAGAEFVGADDLAAE
                     VEGGMLDFDLAIATPDQMPVVGRLGRVLGPRGLMPNPKTGTVTTDVAKAVEEFKGGKV
                     EYRTDRYGNVHVPLGKVSFTPEALEENFRAVLDELQRAKPASAKGRYLKKISVSSTMG
                     PGVKIDSNRLKPEAV"
     gene            3287..3796
                     /gene="rplJ"
                     /locus_tag="VNQ33_09415"
                     /pseudo
     CDS             3287..3796
                     /gene="rplJ"
                     /locus_tag="VNQ33_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            4000..4380
                     /gene="rplL"
                     /locus_tag="VNQ33_09420"
     CDS             4000..4380
                     /gene="rplL"
                     /locus_tag="VNQ33_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWJ98366.1"
                     /translation="MATQEEILDSIANLSVLELSELLKAFEEKFGVTAAAPAAVAVAA
                     GPAAGGGEAAEEQDEFDVILTAAGEKKINVIKEVRALTNLGLKEAKELVDGAPSPILE
                     KASKDDANKAKEALEGAGATVELK"
     gene            4511..>4747
                     /locus_tag="VNQ33_09425"
     CDS             4511..>4747
                     /locus_tag="VNQ33_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98367.1"
                     /translation="MGDGDDGGAEPSRGSGALDLEHGWGPLALLSLAVCLPILLAVVD
                     AARADWVPTADAATIVTRAYDMWGAHRPLVGQFSL"
BASE COUNT          819 a         1741 c         1561 g          626 t
ORIGIN      
        1 gtcgccgcgg aacgtgccga tcgaccggta ggcccggagg taggcgtcct gcaccacgtc
       61 tcgggcgtcg tcgtcatcgg agacgagccg ccgggccagg gtgtaggtgt cggcgctggt
      121 gcgccgcacg agctcctcga aggacccggc atcgcccgcc ttggccgccg cgaccagctc
      181 gtcgaagctg gcgctggaag ggtggatcgg ggggtggacc agccgcatcg ggggttctga
      241 ccccgcgctg gccggcgggg ttaccggctc tgggccgggg tctccggggg gctgatccgc
      301 aggctccacc ccgccagcct ggcacgatcg ggccggtccg ccaccggccg gaggccccgg
      361 aacccgaatt ggcccccttc cagacagggc gtaaggtggt cggccgagca agaaccccca
      421 agggcagtag ctcaattggt agagcaccgg tctccaaaac cggcggttgc gggttcgagt
      481 ccctcctgcc ctgcttttca cattcccgat ccccgatgcg ccgaggcccg tcatggcgat
      541 gaaccgagag cacaagcgga tgctgcagaa gcagggtcag cttgacgcgg aaggcaacca
      601 gaaggccgag cgccgcaagg cgccgtcgcc gaccgcgccc aaagagcccc gcaccaagcc
      661 ccgccagttc gtgcgcgagg tcaacgccga gctccgcaag gtggcctggc cgtcgcgcaa
      721 cgagacgatc agcctctcgg ccatcgtcct cgtgttcctc atcatcatga cggccctcat
      781 cgccgccctc gactacagct tctccgaagc tgtcctctgg atcatcaacc agtaacggtg
      841 ccaaccccat gagcctcgac aaccaagacc tccccaccga cgacgccccg gagaccgacg
      901 acgccgtcgg tgctgcggac gcgcccgagg cggagccgac cggcgacgag gcggccgagg
      961 tcgatgcccc gccagccgtc gaccccgaga cgggcgagat cctcgatccc cccgcggccg
     1021 cggccgccgt cgatgccgag gacctgatcg ccgagccggc gcccgttgtc gacaacccgg
     1081 ccaaccggcc cggcaagtgg tacgtcgtcc acacgcagtc gggctacgag aagaaggtca
     1141 agcagaacct cgaggcccgc acctcgtcca tgaacatgga gggccgcatc aacgaggtgg
     1201 tcatccccat ggaggaggtg accgagttca agaacggcaa gaaggtcatc gtccaaaaga
     1261 agatgttccc cggctacttg ctggtgcgct gcttcctcga cgacgactcc tggtacgtca
     1321 tccgcaacac gcccggcgtc accgggttcg tgggccaggg caccaagccc tcgccgctgc
     1381 ggcgcaagga ggtcgagacc ttcctctcga tccccgtcga gggcgacacg tcgtcgccct
     1441 cggtcggcgc ccgccctgct cgtccccgcc tggagtggga gatcagcgag acggtccggg
     1501 tcaaggaagg accgttcgcc gacttcagcg gcgagatcgt cgagatcgac gaggacaagc
     1561 tcaaggtcaa ggtcctcgtc aacatcttcg gccgggagac ccccgtcgag ctcgacttct
     1621 cccagatcgc caagctctag cccgaccggc ttaggcgagt cgaagcgcgc gcgcgtaggc
     1681 tcgtagaccg gttcgccgcg cgcacccgac tcgccgcacc gattcgtcgg cttcgccgcc
     1741 ggtcaaccca ccgtccgttc attggtcggg ctcgcaagct cgccctccag gtccccaagc
     1801 attcgtagga gcactgagcc accatggcca agaagaaggt caaggccgtc gtcaagatcc
     1861 agatccccgc tggacaggcc acccccgcgc cgcccgtcgg caccgccctc ggcccgcacg
     1921 gtgtggccat catggacttc tgcaaggcgt acaacgccca gaccgaagcc agccgcggcc
     1981 agatcatccc ggtcgagatc acgatcttcg aggatcgctc gttcacgttc atcaccaaga
     2041 ccccgccggc gtcgcggatg ctgctcgaag cagcaggtgt cgcgaaggcc tcgtcgaacc
     2101 ccggcaagga gtccggcggc tcggtcaccg cggcgcagat cgaagagatc gccaaggtca
     2161 agctgcccga cctgaacgcc aacgacctcg acgccgccaa gaagatcgtc gaaggcacgg
     2221 cccgctcgat gggcctcgcg gtcaactgac cacctgatcc accgcacccg caaccgcggg
     2281 tgcatcacga acctccacac gacccaaggc acgaccggga cgacctcgcc cggcggccac
     2341 gaccctgagg gagccaacca tggcgcaagg caagaagtac tccgacgcga tcaagcgcta
     2401 cgacaccgag gccctccacg gcatcggcga ggcgctcggc cacgtgaagg ccctggcccc
     2461 ggcccagttc gacgagaccg tcgagctggt cgtgcggctc ggcgtcgatc cccgcaaggc
     2521 ggaccagatg gtccgcggca cggtcgccct gccggccggc accggcaaag acgtccgcat
     2581 cgcggtgttc gccaccggtg acgccgccgc ggccgcccgt gaggccggtg ccgagttcgt
     2641 cggcgccgac gacctcgccg ccgaggtgga gggcggcatg ctcgacttcg acctggccat
     2701 cgccaccccc gaccagatgc ccgtcgtcgg ccgcctcggc cgggtgctcg gcccccgtgg
     2761 cttgatgccc aaccccaaga ccggcaccgt caccaccgac gtcgccaagg ccgtcgagga
     2821 gttcaagggc ggcaaggtcg agtaccgcac cgaccgctac ggcaacgtgc acgtcccgct
     2881 cggcaaggtg agcttcaccc ccgaggcgct cgaggagaac ttccgggccg tgctcgacga
     2941 gctccagcgg gccaagccgg cttcggccaa gggccgctac ctcaagaaga tctcggtcag
     3001 ctccaccatg ggccccgggg tcaagatcga cagcaaccgc ctcaagcccg aggccgtcta
     3061 gcagtctccg ggggccaccg gctacggtgg cccccgcaca acctgcagta cgccgaagac
     3121 ctccggtgcg ccctcgggcg cgcaatgggc ccccgggccc gccagatgag gcggacgaat
     3181 cgcaagctcg tctgtttcat gggtgttcgc ggaccgcgga cacccatttc gtattccggg
     3241 gcgcctgctc cggaacccac cagaaacaag atgagaggag gtgcccatgg agaacccgag
     3301 gcctgagaag gtcgccgtgg tcgacgaggt ccgagccaag ctcgaggagt cgaacgctgc
     3361 ggtgctgacc gaataccggg ggctcaacgt gtcggccacc gccgatctgc gtcgtgcgct
     3421 gcgcgacgca ggaggcgagt acaagatcta caagaacacc ctggtccgca tcgcggcccg
     3481 ggacctgggc ttggagctcg acgacctgct gaccggcccg acggccatcg ccttcgtcac
     3541 cggcgacgcc gtcggcgtcg ccaaggcgct ccaggacttc gccaagacca acccggccct
     3601 cgtggtcaag ggcggtctgc tgggcgacca gatcctgagc gacaaggacg tgaaggcgct
     3661 cgccgacctg gagccccgcg aggtcctgct cgccaagttc gccggcctgc tcgccgcccc
     3721 gctgcagcag ttcgccggtc tcctcgaggc cctgccgcgg aacttcgcgt acggcctcaa
     3781 ggccctcatc gaccagggcg gcgctcccgg cgctcctgcc gatgccccgg ctgctgctgc
     3841 ggaggaggcc ccggcggccg agtccgaagc cgaagccgcc gctcctgagg cagaggccga
     3901 ggcccctgcc gaagcaaccg ccgcagaggc ccccgccgct gctgctgaag acaccgagac
     3961 cccggccgag tcggccgaca ccgaaagcga gagctgacca tggctaccca agaagagatc
     4021 ctcgattcca tcgccaacct gtccgtcctc gagctctccg agctcctgaa ggcgttcgag
     4081 gagaagttcg gcgtgaccgc cgctgctccc gccgccgtgg ccgtggccgc gggccccgct
     4141 gccggtggcg gcgaggctgc cgaagagcag gacgagttcg acgtgatcct caccgcggcc
     4201 ggtgagaaga agatcaacgt catcaaggag gtgcgcgccc tcaccaacct cggtctcaag
     4261 gaggccaagg agctggtcga cggcgccccc tcgcccatct tggagaaggc ttccaaggac
     4321 gacgccaaca aggcgaagga agccctcgag ggcgccggcg ccaccgtcga gctcaagtag
     4381 atccgagcgg gccgcgcccg ctcaccgtgc acaccgccag cagccgctgc ctccgggcag
     4441 cggctgctgg cgcgaccggg ctgctgggcg cgcgttgggg ccgggggcgg cgctgtgcga
     4501 gcatgcgccg atgggcgacg gggacgacgg cggcgcggag ccgtcccgag ggagcggcgc
     4561 gctcgacctc gagcacggct gggggccgct ggcgctgctg tcgctggcgg tgtgcctgcc
     4621 gatcctgctc gccgtggtcg acgccgcccg cgccgactgg gtcccgacgg ccgacgccgc
     4681 cacgatcgtc acccgggcgt acgacatgtg gggcgcgcac cggccgctcg tcggtcagtt
     4741 ctcgctc
//
LOCUS       DATMFS010000499         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17441, whole genome shotgun sequence.
ACCESSION   DATMFS010000499 DATMFS010000000
VERSION     DATMFS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17441"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="VNQ33_09430"
     CDS             <1..1747
                     /locus_tag="VNQ33_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98368.1"
                     /translation="SDSCMAHTPILVAGGGGGGAGGSGPSDTLTGGGGAGGGGGEVGT
                     RGSGCSTTSASAGTLGLTQPDQAWYGTGPVGTTLGGSGGGGSGCTDKPGGAGGGGGGA
                     GGGAGGDRCCTTASGGAGGTSNAPGGGGAGESGPGADGTAAPVTGGSGGPGGDATSGG
                     GTGGGGGGGYAGGGGGGAGATSRWGLPGGGGGGGSSWANPAIGGTVVLRPNASRNGIA
                     QGEATVVIRGPQLVFGDPGPQTDATGVPVHLDLDIGIGNPHPGTMTRAISAAVLPPGL
                     SIDPQAGSVTGIPTTPGSYPTTITVTSTHSVDGTVSASATFLWTVTGSAVTTSTSIPT
                     STSAPASTSTSTSTSTPASTSTVPCTPDLVPFATPAKLVDQLAADAGKTAAIAPNRAA
                     MITGIADCSLDADDLAVQFVQNPLTQDDARLLRLYVAYFKRPPDPDGFAYWQRQLDAG
                     RGLINAAKKFAESPEFKRTYGTLSNAAFIELVYQNVLGRGSDPTGRAFWLTRLDNKTK
                     NRGDVMINFSESSENLNKKKPHVAVFRLHRAMLRRFPAKTAYDGLLDPVLAGTATLDD
                     VARELRTSPAYAARF"
     gene            1800..>2201
                     /locus_tag="VNQ33_09435"
     CDS             1800..>2201
                     /locus_tag="VNQ33_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWJ98369.1"
                     /translation="MGKDIPLGRIAGIKVGMDVSVFLFATFLAFLLANSQLPSLSHGH
                     STTAYWIAGIVGALFLFLSLLIHEIGHALVAKDEGIGVESMELNLLGGVTRMESSPTT
                     PGAEFRVSVVGPIASATCGVVFLVGSFLLPDH"
BASE COUNT          319 a          850 c          748 g          284 t
ORIGIN      
        1 atccgacagc tgcatggccc acaccccgat cctcgtcgcc ggtggtggtg gcggcggtgc
       61 cggcggcagc gggccctcgg acaccctcac gggaggcggc ggcgcagggg gcggtggcgg
      121 ggaggtcggc accaggggct ccggctgcag caccacgtcg gcatccgccg gcaccctcgg
      181 cctgacgcag cccgaccagg cctggtacgg caccggcccc gtcggcacca cgctcggcgg
      241 cagcggcggg ggcgggagcg ggtgcaccga caagccaggc ggcgccggcg gcggcggagg
      301 cggcgctgga ggcggagccg gcggcgatcg ctgctgcacc acggcgagtg gcggcgcggg
      361 cggcacgagc aacgcacccg gcggcggagg agcaggcgag tccggtcccg gggctgacgg
      421 cacggccgct ccggtcaccg gcggctcagg tggccccggc ggcgacgcca caagcggcgg
      481 gggcaccggc gggggcggcg ggggcggcta tgccggcggt ggcgggggcg gagccggggc
      541 caccagccgg tggggtctcc ccggcggcgg cggaggcggc ggatcgtcgt gggcgaaccc
      601 cgcgatcggg ggcaccgtcg tgttgcggcc gaacgccagc cggaacggca tcgcacaggg
      661 cgaagccacg gtggtgatcc gaggccccca actggtgttc ggtgaccccg gcccccagac
      721 cgacgccacc ggcgtcccgg tgcacctcga cctcgacatc ggcatcggca acccccaccc
      781 cgggacgatg acccgcgcca tcagcgccgc ggtcctcccg cccggcctgt cgatcgatcc
      841 ccaggcaggc tcggtcaccg ggatccccac gacacccggc tcctacccga cgacgatcac
      901 cgtcacgtcg acccacagcg tcgatggcac cgtgagcgcg agcgccacgt tcctgtggac
      961 ggtcaccgga tcggccgtga ccacgagcac gtcgatcccg acctcgacgt ccgcgcctgc
     1021 ctcgacttcg acttcgacct cgacttcgac ccccgcctcg acctcgacgg tgccgtgcac
     1081 gcccgacctg gtcccgttcg ccacgccggc aaagctcgtc gaccagctcg ccgcggacgc
     1141 cggcaagacc gcagccatcg cccccaaccg cgccgcgatg atcaccggca tcgcggactg
     1201 ctcgctcgac gccgacgatc tggccgtcca gttcgtccag aacccgctca cccaggacga
     1261 tgcccgcctc ctgcggctgt acgtggcgta cttcaagcgc ccgccggacc ccgacggctt
     1321 cgcctactgg cagcgccagc tcgacgccgg ccgcggcctc atcaacgcgg ccaagaagtt
     1381 cgccgagtca cccgagttca agcggacgta cggcaccctt tcgaacgccg ccttcatcga
     1441 gctcgtgtac cagaacgtct tgggccgcgg ctcggatccg accggcaggg ccttctggct
     1501 gacccgtctc gacaacaaga ccaagaaccg cggcgacgtg atgatcaact tctccgagtc
     1561 gtctgagaac ctgaacaaga agaagcccca cgtcgccgtg ttccgactcc accgggcgat
     1621 gctccggagg ttcccggcca agacggcgta cgacgggctg ctcgatccgg tcctggccgg
     1681 caccgccacc ctcgacgacg tcgcccgcga gctccggacg tcgccggcgt acgcggcccg
     1741 gttctgaccg cgcaccccgc cgcggggcga gaacccaccg gcgccggtag attcggccga
     1801 tgggcaagga catccccctg gggcgcatcg ccggcatcaa ggtcggcatg gacgtgtcgg
     1861 tgttcctgtt cgccacgttc ctcgcgttcc tgctcgccaa ctcgcagctc ccgagcctga
     1921 gccacggcca ttccacgacc gcctactgga tcgccggcat cgtcggcgcc ctcttcttgt
     1981 tcctctcgct gctgatccac gagatcggcc acgcgctcgt cgccaaggac gaaggcatcg
     2041 gcgtcgagag catggagctc aacctgctcg gcggcgtcac ccgcatggag tcctcgccca
     2101 ccacccccgg agccgagttc cgggtgtcgg tcgtggggcc gatcgccagc gccacctgcg
     2161 gcgtcgtgtt cctcgtcggc tcgttcctgc tgcccgacca c
//
LOCUS       DATMFS010000500         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_111939, whole genome shotgun sequence.
ACCESSION   DATMFS010000500 DATMFS010000000
VERSION     DATMFS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111939"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..697)
                     /locus_tag="VNQ33_09440"
     CDS             complement(227..697)
                     /locus_tag="VNQ33_09440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ98370.1"
                     /translation="MSDRYRLHRLRQGDEWALNTLAKGNARFGDGEDRPWVPPLSSDE
                     ASEFVNDPRTICVVAIHMETNHMAGFAYGGVLYRRHTKLRHLCVYEIGIDIDHRNQGV
                     GKVLLDGIGAEARQMGIDRGFAFASADDRETRAIYDSAGGQHSNDNEILYGFEF"
     gene            complement(848..1630)
                     /locus_tag="VNQ33_09445"
     CDS             complement(848..1630)
                     /locus_tag="VNQ33_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWJ98371.1"
                     /translation="MSDTLSSLVTRADVCAVAVADCFRGDGELLANPIGTIPMIGGRL
                     ARATWEPDLVMTDGEAALIANDEAVPAVDDRRYETYNPYRTMFDVVWSGRRHVMMGAT
                     QVDRFGNQNIAGLGGTPTRPRTQLLGFRGAPGNTINDTTSYWVPQHGPRVFVEHVDIV
                     TGIGYDRASALGDQAARFHEIRRVVSNLGVFDFETPDHRMRVRSLHPGVSVDDVVAAT
                     GFELVIPDDVSTSRLPTPEELRLLTEVIDPKGTRLREVPEPG"
     gene            complement(1630..>2239)
                     /locus_tag="VNQ33_09450"
     CDS             complement(1630..>2239)
                     /locus_tag="VNQ33_09450"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWJ98372.1"
                     /translation="ARQSGAIECTELDEGMLLLGLQAAAWRVPFIPTRVGLGSDLHLV
                     NPGLKTVTSPYGDGEELIAQPAIHLDAALCHLNVADERGNAAFRGPDLYFDDLLLQAA
                     ERRFISTERIVPSGDLVDAAGDITRLRINRLFVDGVVEAPNGAHPTSCEPEYARDEAF
                     QKAYLGTAKEAEAWEAFRSEWLSFPTEADYQAALAARPTGDA"
BASE COUNT          327 a          749 c          829 g          334 t
ORIGIN      
        1 ccccagcaga tcggtcagcc gcgtccgcaa cccatcgcca tcactggtcg aagcagcggt
       61 cgaggcggtc tctgatgacc cgtcagacat gtcgagcagc gtacgacggc acccgcggca
      121 cccgcgaaac cgtccccacc gccctcagtc cgagcttcgc accgctcgca accgagatga
      181 accgctggag cgcggcgctc ggtgggtcgg ctggggtgtg aggccgtcag aactcgaagc
      241 cgtagaggat ctcgttgtcg ttgctgtgct ggccaccggc gctgtcgtag atggcgcgcg
      301 tctcacggtc gtcggccgac gcgaaggcga agccgcggtc gatgcccatc tgccgtgcct
      361 cggcgccgat gccgtcgagc aggaccttgc cgaccccttg gttccggtgg tcgatgtcga
      421 tgccgatctc gtacacgcag aggtggcgca acttggtgtg ccggcggtac agcacgccgc
      481 cgtaggcgaa gccagccatg tggttcgtct ccatgtggat ggccacgacg cagatcgtcc
      541 gggggtcgtt gacgaactcg ctggcctcgt cgctcgacag cggcgggacc cacggacggt
      601 cctcgccgtc gccgaagcgg gcgttgccct tcgcgagcgt gttcagcgcc cattcgtcac
      661 cctgtcgaag ccggtgcagc cggtaccgat cactcaccac agcgcggagc gtaggcgagc
      721 gacgacccac tgcgtcgcca atcagacatc caacccgccc ccggatcgga ctccgtcggc
      781 gagaacgccc gaacgagtgc cccgacctgc gaggttcgca ccggtcgggt ggcgcggcgc
      841 cggtgggtca ccccggctcg gggacctcgc ggaggcgggt gcccttgggg tcgatgacct
      901 cggtgaggag gcggagctcc tcgggcgtcg ggagacggga ggtggacacg tcgtcgggga
      961 tgaccagctc gaagccggtg gcggcgacca cgtcgtcgac cgagaccccg gggtgcagcg
     1021 agcgcacgcg catccggtga tcgggcgtct cgaagtcgaa gacgccgagg ttcgacacga
     1081 cccgccggat ctcgtggaag cgggccgcct ggtcccccag tgctgacgcc cggtcgtagc
     1141 cgatgcccgt gacgatgtcg acgtgctcga cgaacacccg cgggccgtgc tggggcaccc
     1201 agtacgacgt ggtgtcgttg atcgtgttgc ccggcgcgcc gcggaacccg aggagctggg
     1261 tccggggccg ggtcggcgtg ccgccgaggc cggcgatgtt ctggttgccg aaccggtcca
     1321 cctgggtggc gcccatcatc acgtgacgcc gcccgctcca gaccacgtcg aacatggtgc
     1381 ggtacgggtt gtaggtctcg tagcggcggt cgtcgacggc ggggacggcc tcgtcgttgg
     1441 cgatgagggc ggcctcgccg tcggtcatca ccaggtccgg ctcccacgtg gcgcgggcga
     1501 ggcggccgcc gatcatgggg atggtgccga tggggttggc gagcagctcg ccatcgcccc
     1561 ggaagcagtc ggcgacggcg acggcgcaca cgtcggcgcg ggtcacgagc gaggacaggg
     1621 tgtcggacat caggcgtctc cggtggggcg ggcggccagg gcggcctggt agtcggcttc
     1681 ggtcgggaac gacagccact cggaccggaa ggcctcccac gcctcggcct ccttggccgt
     1741 gcccaggtac gccttctgga aggcctcgtc ccgggcgtac tcgggttcgc acgacgtggg
     1801 gtgggcgccg ttgggtgcct cgaccacacc atccacgaac aggcggttga tgcggaggcg
     1861 ggtgatgtcg ccggcggcat cgaccaagtc acccgaaggg acgatccgct cggtcgagat
     1921 gaagcgccgc tcggccgcct gcaggagcag gtcgtcgaag tagaggtccg gtccccggaa
     1981 ggcggcgttg ccccgctcgt cggccacgtt caggtggcag agggcggcat cgaggtggat
     2041 cgccggctgg gcgatcagct cctcgccgtc gccgtagggc gaggtgacgg tcttgagccc
     2101 cgggttgacc aggtgcaggt ccgaccccag gcccacgcgg gtcgggatga acgggacccg
     2161 ccacgccgcc gcctggaggc cgagcagcag cattccctcg tcgagctcgg tgcactcgat
     2221 cgcaccggac tggcgggcg
//
LOCUS       DATMFS010000501         7480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_297059, whole genome shotgun sequence.
ACCESSION   DATMFS010000501 DATMFS010000000
VERSION     DATMFS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297059"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VNQ33_09455"
     CDS             complement(<1..618)
                     /locus_tag="VNQ33_09455"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWJ98373.1"
                     /translation="MTGHRSVAVEVPESLVGERIDRIVAMVTDVSRTEAARLVAAGAV
                     TLDGTVATRGADRLVAPGALAIELGDAAAVAALEPDPSVAISSVYIDEHVIVVDKPAD
                     LVVHPGAGHTTGTLANGLIAAYPEMAAVGEPDRPGIVHRLDRGTSGLLMVARSAVAYE
                     SLTEQLAERTVDRRYRTVVWGHVESPRGVVDAPIGRSTKHPTKMAV"
     gene            complement(615..1229)
                     /gene="lspA"
                     /locus_tag="VNQ33_09460"
     CDS             complement(615..1229)
                     /gene="lspA"
                     /locus_tag="VNQ33_09460"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWJ98374.1"
                     /translation="MVDDLPTDTEAPEESTDALTPDDEAPAASDGSTGAAADGADGSG
                     EPGAPRPPRRLKIVGAVAAAVLIADQASKWWALGRLDDGQIVDLFWTLRFNLFSNTGV
                     AFSLGTDSGLGPWISLLAIGVIIAVSFGTTSRYPLGAVASGLISGGALGNLMDRAFRG
                     DEGFLHGAVIDFIDLQWWPVFNVADAAIVVGALLLVAASFRVPQ"
     gene            complement(1239..1880)
                     /locus_tag="VNQ33_09465"
     CDS             complement(1239..1880)
                     /locus_tag="VNQ33_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HWJ98375.1"
                     /translation="MGTATNKAAPAKKAAAKKVPAKKAPVKKAAAKKKAAAKTPTAKK
                     AAKKAPAKKAAPAAKTVAKKTVANKTVGKKAPAKKAPAKKAGAKSSFGQRWLDSQQAL
                     LEHERATYLHQAESLEAEARDLVADYESGDTQFDEESGEGDTLSMERERDLQLSAQAR
                     AAIEEIDHALAKFALGTYGICEQSGLPIPKERLEAIPWARERVEFKVGGFGHR"
     gene            complement(1898..3130)
                     /locus_tag="VNQ33_09470"
     CDS             complement(1898..3130)
                     /locus_tag="VNQ33_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWJ98376.1"
                     /translation="MELTLKRLLENAEFKKYRGKGYDPTEVDDFLDRATAMAAKVEVQ
                     LTQALEQARSAGGASGPGPDPAAVEAEIDRRVATEVAAQLAAQPAPSTGPSEEEVAAE
                     AQRTLVMAQRTADAAVREARDDADKMVAAAKEQAEALLADAEARASATNSESEQRAAS
                     TRAEVEAEAARERREARQRLAAEIGELEGLRESLRSDVSVLERHVEEQRSQLGSTLAE
                     LQQILDDPSGFRLAPAPALSDPALPDFSDLDEPEPAAAASEPVDAEPVAVAPTPSADE
                     PEPEPEPEAAVADEPPAPELPPVVAPEPVAAPGEAEPPVPPVLAFEEVDHEAPGLDDP
                     PDSGPPTAPVSAVDLGLAPERAPSAEGGDEDAFLTELRKAMADEEPLGPRDAGDIPPT
                     ESLFGEQDRRGFRFGKRR"
     gene            complement(3186..3767)
                     /gene="sepF"
                     /locus_tag="VNQ33_09475"
     CDS             complement(3186..3767)
                     /gene="sepF"
                     /locus_tag="VNQ33_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HWJ98377.1"
                     /translation="MWRKAMVYLGLGDDAEYDEYGSNYDDEPERSNPMSRDRPPVRSQ
                     AAPPASSYPDEPSGIGSVRPISTRGVDPREPDTGQTVAPTGVGQVRPRPQVVRPMPVM
                     PNAKPFVVMPTSFNQAQDVADKYKGSQPVIMNLQGADRDLSRRLIDFASGLCYGLGGQ
                     MERVANQVYLLTPTNVEVSPEERRRLHERGYDT"
     gene            complement(3778..4467)
                     /locus_tag="VNQ33_09480"
     CDS             complement(3778..4467)
                     /locus_tag="VNQ33_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWJ98378.1"
                     /translation="MSAPEPDVLLARVEQVRNRIADAGGDPAAVVLLAVTKGFGPEVA
                     MAAASAGLTDLGENYAQELVAKAPVLADAGLAPRWHAIGRLQRNKVKALAGIVSLWQT
                     VDRIDLGQEIAKRAPAAPVLVQVNVSDEPQKGGCAPDEAPDLVARLGDAGLDVQGLMT
                     VGRTGPAAAARAGFAVLRGLADDLDLPVRSMGMSGDLEAAVAEGSTMVRVGSALFGPR
                     PSVPGPAAAPR"
     gene            complement(4544..5191)
                     /locus_tag="VNQ33_09485"
     CDS             complement(4544..5191)
                     /locus_tag="VNQ33_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HWJ98379.1"
                     /translation="MTGEVRSVFTDTTHGDLMVRAEPSALAARRAAVVDRPWSWLRQV
                     HGARVVTVTEPGQHAGDEADAAVTAVPGAVLAVQTADCAPVLLTGPGVVGVAHAGWRG
                     LEAGVVEATAAAMADLGGAPEHAALGPCIRARCYEFGGTELDLVAARYGDAVRATTAW
                     GTPALDVAAGIAEACRRLGIELHDVGTCTACSPNHWSHRARAEPQRQALVAWIEP"
     gene            complement(5202..6338)
                     /gene="ftsZ"
                     /locus_tag="VNQ33_09490"
     CDS             complement(5202..6338)
                     /gene="ftsZ"
                     /locus_tag="VNQ33_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWJ98380.1"
                     /translation="MAGNPQNYLAVIKVVGVGGGGVNAVNRMIDAGLKGVEFIAVNTD
                     AQALLMSDADIKLDIGRELTRGLGAGSDPEVGRLAAEEHRNEIEEVLQGADMVFITAG
                     KGGGTGTGAAPVIAEIAKGIGALTIGVVTRPFAFEGRRRSVQAEAGIQKLKEKVDTQI
                     VIPNDRLLTVSNEKTSMMNAFKMADEVLLQGVQGITDLITTPGVINTDFADVKMIMSN
                     AGSALMGIGTATGEGRALNAARGAISSPLLEASIEGARGILLSIAGGSDLGLFEVNEA
                     AEVIHGVAHPDANIIFGTVIDDEMGDEVRVTVIAAGFDRWEEKTSRPSRREEGAASGD
                     APPPRRPPRPSSASDLFADSAEHRLGDDEGGGGDDEFDVPSFLK"
     gene            complement(6471..7355)
                     /locus_tag="VNQ33_09495"
     CDS             complement(6471..7355)
                     /locus_tag="VNQ33_09495"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWJ98381.1"
                     /translation="MTETVFRQRSKPAKAGSKPSRAAKATTSATAPKAAKEPKAKAKA
                     KPESPSLVDPRLQARRIEVARGEGRRRLRWLATALAVAGLAAGAFALTESPLLDVDAV
                     AVKGASRTEQAEAQKASGIALGAPMAGVDASAAERRLEALPWVERATVSRSWPGTVEI
                     TLVERTPIAVVGTGAGALEVDRAGRVLGPAAGGGLPVLAGAAAPVGDDLPAPQRSALA
                     TLSGLPAPLRSEVAGVVATSSGVRFALADDIEVWWGDRSQPSAKADALQVLLEQADRD
                     TIATIDVSAPRAATVTRK"
BASE COUNT          945 a         2666 c         2794 g         1075 t
ORIGIN      
        1 gacggccatc ttcgtggggt gcttcgtcga ccggccgatg ggggcgtcga cgacgccgcg
       61 gggcgattcg acgtggcccc acacgaccgt gcggtaccga cggtcgacgg tgcgctcggc
      121 cagctgctcg gtgagcgact cgtaggccac ggcgctgcgg gccaccatca gcagcccgga
      181 ggtgccgcgg tcgaggcggt ggacgatgcc cggacgatcc ggctcgccga ccgccgccat
      241 ctcggggtag gcggcgatca agccgttggc caaggtgccg gtcgtgtggc cagcaccggg
      301 gtggaccacc aggtcggccg gcttgtccac cacgatgacg tgctcgtcga tgtacaccga
      361 cgagatggcg acgctcgggt cgggctccag cgcggccacg gcggccgcgt cgccgagctc
      421 gatggccagc gcgcccggag cgacgagccg atcggcgccg cgggtggcca cggtgccgtc
      481 gagcgtcacc gcgcccgccg ccaccagccg cgccgcttcg gtgcggctca cgtcggtgac
      541 catggcgacg atccggtcga tgcgctcccc cacgagcgac tcgggcacct cgaccgccac
      601 cgagcggtgg ccggtcactg gggcactcgg aagctggcgg ccaccaggag gagcgccccg
      661 accacgatgg cggcgtcggc gacgttgaac accggccacc actgcaggtc gatgaagtcg
      721 atgacggcgc cgtggaggaa cccctcgtcg ccgcggaagg cccggtccat caggttgccc
      781 agcgccccac ccgagatcag gcccgacgcg acggcgccca gcgggtaccg gctggtggtc
      841 ccgaacgaga cggcgatgat cacgccgatg gcgagcagcg agatccacgg cccgaggccg
      901 ctgtcggtgc cgaggctgaa cgcgacgccg gtgttggaga agaggttgaa ccgcagcgtc
      961 cagaacagat cgacgatctg gccgtcgtcg agccggccga gcgcccacca cttcgacgcc
     1021 tggtcggcga tgagcaccgc ggcggcgacc gcgccgacga tcttcagccg acggggcgga
     1081 cgcggcgcgc cgggctcgcc ggagccgtcc gcgccgtccg cagcagcgcc ggtgctgccg
     1141 tcggacgcgg cgggagcctc gtcgtcgggc gtcagggcat cggtggactc ctcgggagcc
     1201 tccgtgtcgg tgggcagatc gtcgaccatg ggctcgacct agcggtggcc gaatcctccg
     1261 accttgaact cgacgcgctc gcgcgcccac gggatcgcct cgaggcgctc cttcgggatc
     1321 ggcagcccgg actgctcgca gatgccgtag gtgccgagcg cgaacttcgc cagcgcgtgg
     1381 tcgatctcct cgatggcggc ccgggcctgg gccgagagct gcagatcgcg ctcgcgctcc
     1441 atgctgagcg tgtcgccctc gcccgactcc tcgtcgaact gggtgtcgcc ggactcgtag
     1501 tcggccacga ggtcgcgtgc ctccgcctcc aggctctccg cctggtgcag gtaggtggcg
     1561 cgctcgtgct cgaggagcgc ctgctgcgag tcgagccacc tctgcccgaa cgacgacttc
     1621 gcgcccgcct tcttcgccgg agccttcttc gccggggcct tcttgcccac ggtcttgttg
     1681 gcgacggtct tcttcgccac ggtcttggcc gccggagcgg ccttcttggc aggagccttc
     1741 ttggcagcct tcttggccgt cggggtcttg gccgccgcct tcttcttggc cgccgccttc
     1801 ttcaccggag ccttcttcgc gggcaccttc ttcgcggctg ccttcttcgc tggtgcggcc
     1861 ttgttcgtcg ctgttcccac ccgggcagcc atcggtgtca gcggcgcttg ccgaagcgga
     1921 agccgcgtcg atcctgctcg ccgaacaggg actcggtcgg cgggatgtcg ccggcgtcac
     1981 gcggaccgag gggctcctcg tcggccatgg ccttgcggag ctccgtgagg aacgcatcct
     2041 cgtcaccgcc ctcggcggac ggggcccgct cgggcgccag gccgaggtcg acggcgctca
     2101 ccggcgccgt cgggggcccg ctgtcgggcg ggtcgtcgag gccgggcgcc tcgtggtcga
     2161 cctcctcgaa ggcgagcacc gggggaaccg gcggctccgc ttcgcccggg gctgcgaccg
     2221 gctcgggagc gacgaccggc ggaagctccg gggccggggg ctcgtcggcc acggcggcct
     2281 cgggttcggg ctcgggctca ggctcgtcgg cggaaggcgt cggcgcgacc gccaccggct
     2341 cggcgtcgac gggctcggag gcggcggccg ctggctcggg ctcgtcgaga tcggagaagt
     2401 ccgggagcgc cgggtcgctc agtgccgggg ccggagcgag ccggaagccc gacgggtcgt
     2461 cgaggatctg ctgcagctcg gcgagggtcg agccgagctg cgaccgctgc tcctcgacgt
     2521 ggcgctcgag cacgctcacg tcgctgcgca gcgactcgcg caggccctcg agctcgccga
     2581 tctcggcggc caggcgctga cgggcttccc gccgctcccg ggctgcttcg gcttcgacct
     2641 cggcccgggt ggaagccgcc cgctgctcgg actcgctgtt ggtggccgag gcgcgggcct
     2701 cggcgtcggc gaggagggcc tcggcctgct ccttggcggc cgcgaccatc ttgtcggcgt
     2761 cgtcgcgggc ttcgcggacg gcggcatcgg cggtgcgctg ggccatgacg agcgtgcgct
     2821 gcgcctcagc ggcgacctcc tcctccgacg gcccggtgga gggggcgggc tgcgcggcga
     2881 gctgggcggc gacctcggtg gccacccgcc ggtcgatctc ggcttcgacg gccgcgggat
     2941 cggggccggg gccggacgcg ccaccagccg acctggcttg ctccagggcc tgcgtgagct
     3001 gcacctcgac cttggccgcc atcgccgtcg cccggtccaa gaagtcgtcg acctcggtgg
     3061 ggtcgtagcc ctttcctcgg tacttcttga actcggcgtt ctcgagcagg cgcttcaggg
     3121 tcaactccat gccgccaccc tacccgtgtg cttcgggccg ggccgcgaca cgtccccggg
     3181 ctcgatcagg tgtcgtagcc gcgctcgtgc aggcgccgac gctcctcggg cgacacctcc
     3241 acgttggtgg gcgtgagcag gtacacctgg ttggccaccc gctccatctg cccgccgagg
     3301 ccgtagcaga gaccgctcgc gaagtcgatg agccggcggg acaggtcccg gtcggcgccc
     3361 tggaggttca tgatgacggg ctggctgccc ttgtacttgt ccgccacgtc ttgagcttgg
     3421 ttgaaggacg tcggcatgac cacgaacggc ttggcgttgg gcatgaccgg catgggccgc
     3481 accacctggg gacggggacg gacctgaccc accccggtgg gtgcgacggt ctggccggtg
     3541 tcgggctcac gggggtcgac gccgcgggtg ctgatgggac ggaccgagcc gatgcccgag
     3601 ggctcgtcgg ggtaggacga cgccggcggt gcggcctggc tgcggacggg cgggcggtca
     3661 cggctcatgg ggttgctgcg ctccggctcg tcgtcgtagt tgctgccgta ctcgtcgtac
     3721 tcagcgtcat cgccgagccc gaggtacacc attgccttgc gccacatgca cgtgaggtta
     3781 gcggggagcg gccgcaggac cagggacgga cggtcgcgga ccgaacaggg ctgaaccgac
     3841 gcggaccatc gtcgagccct cggcgacggc cgcctcgagg tcgccggaca tgcccatcga
     3901 cctcaccggg aggtcgaggt cgtcagccag gccgcggagc acggcgaacc cggcccgggc
     3961 cgcggcggcc ggtccggtgc ggcccaccgt catcagcccc tgcacgtcga ggccggcgtc
     4021 gccgagcctg gccacgaggt cgggagcctc gtcgggggcg cacccgccct tctgcggctc
     4081 gtcggacacg ttgacctgca cgaggaccgg ggcagccgga gcccgcttgg cgatctcctg
     4141 gccgaggtcg atgcggtcca ccgtctgcca gagcgagacg atcccagcca gggccttcac
     4201 cttgttgcgc tgaaggcgcc cgatggcgtg ccagcgcggc gccaggcccg cgtcggccag
     4261 caccggggcc ttggccacca gctcctgggc gtagttctcg cccaggtcgg tgaggccggc
     4321 cgaagcagcc gccatcgcca cctcgggacc gaaccccttg gtgacggcca gcagcaccac
     4381 ggcggcgggg tccccaccgg catcggcgat gcggttgcgc acctgctcga cccgggcgag
     4441 caggacgtcg ggctccggtg cgctcacgac tccggacctc cagcagacgt ggaggggcga
     4501 gctgaggtcg aggtcgaggg ccaacccgaa gtcgaggtcg aggtcacggc tcgatccaag
     4561 cgaccagggc ctggcgctgc ggttcggcgc gggcccggtg cgaccagtgg ttggggctgc
     4621 acgccgtgca ggtgccgacg tcgtgcagct cgatgcccag gcggcggcag gcctcggcga
     4681 tgccggcggc gacgtcgagc gccggggtgc cccacgccgt cgtggcgcgg acggcgtcgc
     4741 cgtagcgggc cgcgacgagg tccagctcgg tgccaccgaa ctcgtagcag cgggcccgga
     4801 tgcacgggcc gagcgcggcg tgctcgggcg ccccgccgag gtcggccatc gccgcggccg
     4861 tggcctcgac gacacccgcc tccaggcccc gccacccggc gtgggccacc ccgacgacgc
     4921 cgggtccggt gaggagcacg ggagcgcagt cggcggtctg gacggcgagc acggcgccgg
     4981 gcacggcggt gacggcggcg tcggcttcgt cccccgcgtg ctggcccggc tcggtgacgg
     5041 tgacgacccg ggcgccgtgc acctggcgca gccaggacca cggccggtcg acgacggccg
     5101 cacggcgcgc cgcgagggcc gacggctcgg cgcggaccat caggtcgccg tgggtggtgt
     5161 cggtgaagac cgagcgaacc tcgccggtca cggcgtcggc gctacttcag gaacgacggg
     5221 acgtcgaact cgtcgtcacc gccaccgcct tcgtcgtcgc ccaggcggtg ctcggcggag
     5281 tcggcgaaca ggtcgctggc gctcgacgga cggggcggcc gccgcggcgg cggggcatcg
     5341 cccgaggcgg cgccctcctc acggcgcgac gggcgggagg tcttctcctc ccagcggtcg
     5401 aagccggcgg cgatgaccgt gacccggacc tcgtcgccca tctcgtcgtc gatcacggtg
     5461 ccgaagatga tgttggcgtc ggggtgcgcc acgccgtgga tcacctcggc ggcctcgttg
     5521 acctcgaaca ggccgaggtc cgagccgccg gcgatcgaga gcaagatgcc ccgggcgcct
     5581 tcgatggacg cctcgagcag cgggctcgag atggcgccgc gggcggcgtt gagcgcccgg
     5641 ccctcgccgg tggcggtgcc gatgcccatg agggccgagc cggcgttgct catgatcatc
     5701 ttcacgtcgg cgaagtcggt gttgatgacg cccggcgtgg tgatgaggtc ggtgatgccc
     5761 tggacgccct ggagcaggac ctcgtcggcc atcttgaagg cgttcatcat cgacgtcttc
     5821 tcgttcgaca cggtgagcag ccggtcgttc gggatgacga tctgggtgtc gaccttctcc
     5881 ttcagcttct ggatgccggc ctcggcctgc acggagcggc ggcggccctc gaaggcgaac
     5941 gggcgggtga ccacgccgat ggtcagcgcg ccgatgccct tggcgatctc ggcgatgacc
     6001 ggggccgcac cggtgccggt gccgccgcct ttgcccgcgg tgatgaacac catgtcggcc
     6061 ccctggagga cctcctcgat ctcgttgcgg tgctcctcgg cggccaggcg gccgacctcg
     6121 gggtcgctgc cggcaccgag gccccgggtg agctcacggc cgatgtcgag cttgatgtcc
     6181 gcgtccgaca tcagcagcgc ctgcgcgtcg gtgttgacag cgatgaactc gacgcccttg
     6241 aggccagcgt cgatcatgcg gttgacggcg ttgacgccac caccgccgac accgaccacc
     6301 ttgatgacgg cgagatagtt ctgaggatta ccggccatgg ctgctacctc cacacggggt
     6361 ggttggaagg gaaaccctca acgctggctg gagaaggggt gaaccttgag gaacggttga
     6421 cattagtacc caatcattgc gggtcagagg ggcggagcgg gggattccgt tcacttccgg
     6481 gtcacggtgg cggcccgggg agcgctgacg tcgatggtgg cgatggtgtc gcggtcggcc
     6541 tgctcgagca ggacctgaag ggcgtctgcc ttggcgctcg gctggctgcg gtccccccac
     6601 cagacctcga tgtcgtcggc gagcgcgaac cggacacccg acgaggtggc gacgaccccg
     6661 gccacctcgg agcggagcgg ggccggcaga cccgagagcg tggcgagcgc cgagcgctgc
     6721 ggggccggga ggtcgtcgcc gacgggcgcg gcggccccgg cgaggacggg caggccgccg
     6781 ccggcggcag ggccgagcac gcgtccggcg cgatccacct cgagcgcgcc ggcgccggtc
     6841 cccacgaccg cgatcggcgt gcgctcgacg agcgtgatct cgacggtgcc gggccacgag
     6901 cgcgacaccg tggcccgctc gacccagggc agcgcctcga gccggcgttc ggcggcggag
     6961 gcatccaccc ccgccatcgg cgcgcccagg gcgatgcccg atgccttctg cgcctcggcc
     7021 tgctcggtgc gcgaggcgcc cttgaccgcg acggcgtcca cgtccagcag cggcgactcc
     7081 gtgagggcga acgccccagc cgcgagaccg gccacggcca gcgcggtcgc caaccagcgc
     7141 agccgcctgc ggccctcccc gcgggcgacc tcgatccgac gggcctgcag ccgcgggtcc
     7201 accagcgacg ggctctccgg cttcgccttc gctttcgcct tgggttcctt ggccgccttc
     7261 ggagccgttg cggacgtggt ggccttcgcc gcccgagacg gcttcgaccc ggccttcgcc
     7321 ggcttcgacc gctggcggaa gaccgtctcg gtcacgacac cagctccggc atggccgcga
     7381 agcccaccag gcacgtctcg acgtgcagga cgatgccggt ctgctcgtgg acccggcgac
     7441 ggacctcgac catcagatcc cagatgtcct gggcgcggcc
//
LOCUS       DATMFS010000502         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_133448, whole genome shotgun sequence.
ACCESSION   DATMFS010000502 DATMFS010000000
VERSION     DATMFS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133448"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VNQ33_09500"
     CDS             <1..853
                     /locus_tag="VNQ33_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98382.1"
                     /translation="QFLVGPLGWVALAATMTATARASASRVADLITTSPAVADGADPV
                     PAAPAPGHPSIELSGVCHRSLDGLDLAVGDGSFLAVAAASAADSEALVDLLGRWHDPE
                     AGTIRLHGAEVTRLPIDAVRRQVLVSEHDAALFEGTVRQAIDGGQPDDGPIAIDAVLA
                     ASGVDEMAGSLPDGLATPISERGLSLSGGQRQRLVLARALAADRPVLVLQEPTTAVDA
                     VTEDRIARGIRELRDGRTTIVVTTSPALLATADEVVFVADGAIAARGTHEDLLAASEP
                     YRTLVTR"
     gene            850..2595
                     /locus_tag="VNQ33_09505"
     CDS             850..2595
                     /locus_tag="VNQ33_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98383.1"
                     /translation="MTAVLLPTASAAATRHALVTRLKPHRIALASSAALFLVRAVAGL
                     VLPLALGLIVNVAVDGDGAGAITAPVLAIVLATLVEGAAAVGGTAVAARAFLPALADL
                     REEVLDRALQVPLADVERAGIGDLTARVDGDVAAVNEAIQEAFPTVLESTLAIAMTIV
                     GLAVLDWRLALAGLGAAPIQFHTMRWYLPRSSPLYAEERVAAGRRSQQLLETVGGAST
                     VQALDLADEHLVLVHDRSRDALRLSLAATWLRTRFFARLNLAEVTGMVLVLVVGFWLS
                     DEHGLSAGAVTAAVLLFQRLFNPINALLYLVDEAQSAGAALARLIGVTAMDPPADEVS
                     PADPADAAVELHDVCFGYRPGHDVLTGLDLRVADGETVALVGATGAGKSTVGRLVAGI
                     AAPAGGTVTIGGIPVAELGDDRARLPVVLVTQEIHLYAGTLADDLRMVRPDATDDELW
                     AALATVGATEWVQGLTDGLDTAVGDGAVELDASRIQLLALARVVLADPMVVVLDEATA
                     EAGSAGARLLDRAAAAALAGRTGLLIAHRLSQAAAADRVVVLDAGRVVEAGTHDELAA
                     AGGVYAGLWHAWRTS"
     gene            2680..>3411
                     /gene="modA"
                     /locus_tag="VNQ33_09510"
     CDS             2680..>3411
                     /gene="modA"
                     /locus_tag="VNQ33_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWJ98384.1"
                     /translation="MRIRRSLLALVAGTALLLAACGSSGGSESSDTTKAPSSSDATTT
                     TAGEPALEGEITVSAAASLTEPFTDMGKAFEDANPGTKITFTFDSSGTLSQQILDGAP
                     VDVFASADEKNMEKLTDADLVKGEPTVFARNQLIIVTKPGNPEGIESLADLADAGIIS
                     LCGEDVPCGKFAGEALENAGVTIPEGSVTRGQNVKATLAAVTEGDAVAGIVYVTDALT
                     AGDAVDAVDIPEDQNVIATYPVAVLT"
BASE COUNT          418 a         1366 c         1153 g          474 t
ORIGIN      
        1 ccagttcctc gtcgggccgc tcggctgggt cgcgctggcc gccaccatga ccgccaccgc
       61 ccgggcgtcg gccagccgcg tcgccgacct gatcaccacg tcgccagcgg tggccgacgg
      121 ggccgatccg gtccccgctg ctcccgcccc tggtcatccg tcgatcgagc tctccggggt
      181 ctgccaccgc agcctcgacg gcctcgacct cgccgtcggt gatggatcgt tcctggcggt
      241 ggccgccgca tcggctgcgg actccgaggc gctggtcgac ctgctcggcc ggtggcacga
      301 ccccgaagcg ggaaccatcc ggctgcacgg cgccgaggtg acccgcctgc cgatcgatgc
      361 cgtgcgccgc caggtcctcg tgagcgagca cgacgccgcc ctgttcgagg gcacggtccg
      421 ccaggcgatc gacggcggcc agcccgacga cggcccgatc gcgatcgatg ccgtcctcgc
      481 cgcctccggc gtggacgaga tggcaggctc gctccccgac ggcctcgcca ccccgatcag
      541 cgagcgcggc ctgtcgctct cgggcgggca gcgccagcgc ctggtgctgg cccgtgccct
      601 agccgccgac cgtccggtgc tcgtgctcca ggagcccacc accgcggtcg acgccgtcac
      661 cgaggaccgc atcgcacgcg gcatccgcga gctgcgcgac ggacggacca ccatcgtggt
      721 gacgaccagc ccggccctgc tcgccacggc cgacgaggtg gtgttcgtcg ccgacggcgc
      781 catcgccgcc cgcggcacgc acgaggacct gctcgccgcg agcgagccgt accgcacgct
      841 ggtgacgcgg tgaccgccgt gctcctgccc accgcctccg ccgccgccac ccggcacgcg
      901 ctcgtcaccc ggctgaagcc ccaccggatc gcgctggcct cgtcggccgc gctgttcctc
      961 gtccgggccg tcgccgggct ggtgctcccc ctcgccctcg gcctgatcgt gaacgtcgcc
     1021 gtcgacgggg acggggccgg ggccatcacc gctcccgtcc tggcgatcgt gctcgccacg
     1081 ctggtcgagg gcgccgccgc ggtcggcggg accgccgtcg cggcacgggc cttcctcccc
     1141 gcgctcgccg acctccgcga ggaggtcctc gaccgagccc tgcaggtgcc gctggcggat
     1201 gtcgagcgcg ccggcatcgg cgacttgacc gctcgggtcg acggcgacgt cgccgccgtg
     1261 aacgaggcga tccaagaggc gttccccacc gtcctcgagt cgacgctcgc catcgccatg
     1321 acgatcgtcg gcctcgccgt cttggactgg cgcctcgccc tcgccggcct gggcgcggcg
     1381 ccgatccagt tccacaccat gcggtggtac ctgccgcgct cctccccgct ctacgccgag
     1441 gagcgggtgg ccgccgggcg gcgctcgcag cagctgctgg agacggtggg cggcgccagc
     1501 accgtgcagg ccctcgacct ggccgacgag cacctcgtcc tcgtgcacga ccgctcccgg
     1561 gacgccctcc gcctctcgct cgccgccacc tggctgcgga cccgcttctt cgcccggctc
     1621 aacctggccg aggtcaccgg catggtgttg gtgctggtgg tcgggttctg gctctccgac
     1681 gagcacggcc tgtcggccgg tgccgtgacc gccgccgtcc tcttgttcca gcgcctcttc
     1741 aacccgatca acgccctgct ctacctcgtc gacgaggccc agtccgccgg tgccgccctc
     1801 gcccggctga tcggtgtcac cgccatggac ccgccggccg acgaggtgtc gcccgccgac
     1861 ccggccgatg cggccgtcga gctgcacgac gtctgcttcg gctaccggcc cggtcacgac
     1921 gtgctcaccg gcctcgacct gcgggtggcc gacggcgaga ccgtggccct cgtcggcgcg
     1981 accggcgccg gcaagagcac ggtcggccgg ctcgtcgccg gcatcgcggc gcccgccggc
     2041 ggcacggtca ccatcggcgg catcccggtg gccgagctgg gcgacgaccg ggcgcggctc
     2101 ccggtcgtgc tcgtcaccca ggagatccac ctctacgccg gcaccctcgc cgacgacctc
     2161 cgcatggtcc gccccgatgc gaccgacgac gagctgtggg ccgcgctcgc caccgtcggc
     2221 gccaccgagt gggtccaggg cctcaccgac ggcctggaca ccgcggtcgg cgacggcgct
     2281 gtggagctgg acgcgagccg catccagctc ctcgccctgg cccgggtcgt cctggccgac
     2341 ccgatggtgg tcgtgctcga cgaggccacc gccgaggcgg gcagcgccgg tgcccgcctc
     2401 ctcgaccggg ccgcggccgc cgcgctcgcg ggccggaccg gtctcctgat cgcccaccgg
     2461 ctcagccagg ccgccgctgc cgaccgggtg gtggtgctcg atgccggccg ggtggtcgag
     2521 gccggcaccc acgacgagct ggccgccgcc ggtggcgtct acgccggcct gtggcacgcc
     2581 tggcggacgt cttgaacaca gcaacggcga ggtacatcga tgttcttcct tgcgtgtgcg
     2641 gggtaggttg ccaccaccct gtctgaggag atctgtccca tgcgcatccg tcgatccctg
     2701 ctggcgctgg ttgccggcac cgccctcctc ctcgccgcct gcggcagcag cggggggtcg
     2761 gagagctcgg acaccaccaa ggcgccgtca tcctccgatg cgacgaccac cacggccggg
     2821 gagccggcgc tcgaaggcga gatcacggtg tcggccgcgg cctcgctcac cgagcccttc
     2881 accgacatgg gcaaggcgtt cgaggacgcc aaccccggca ccaagatcac cttcacgttc
     2941 gactcgtcgg gcacgctctc gcagcagatc ctcgacggtg cccccgtcga cgtcttcgcc
     3001 tcggccgacg agaagaacat ggagaagctc accgacgccg acctggtgaa gggcgagccc
     3061 accgtcttcg ccaggaacca gctcatcatc gtcaccaagc cgggcaaccc cgagggcatc
     3121 gagtccctgg ccgatctcgc cgacgccggg atcatctccc tgtgcggtga agacgtgccg
     3181 tgcgggaagt tcgccggcga ggcgctcgag aacgccgggg tcaccatccc cgagggctcg
     3241 gtcacccgcg gccagaacgt caaggccacc ctcgccgctg tgaccgaggg cgacgccgtg
     3301 gccggcatcg tctacgtcac cgatgccctc acggccgggg acgccgtcga cgccgtcgac
     3361 atccccgagg accagaacgt gatcgccacc taccctgtgg ccgtgctgac c
//
LOCUS       DATMFS010000503         4696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17524, whole genome shotgun sequence.
ACCESSION   DATMFS010000503 DATMFS010000000
VERSION     DATMFS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17524"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3295)
                     /locus_tag="VNQ33_09515"
     CDS             complement(<1..3295)
                     /locus_tag="VNQ33_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWJ98385.1"
                     /translation="MSLFDFLDEPFDETGGSSAPESDPTSDPTPGLEPNAEPDPDPEP
                     DPEAVAEPEPEPGAEAEAEAEAEEAEAEPEAEAEAGAEAVSAAAALVDERPPPPDQDQ
                     REVVRKALGTTLFVEAGAGSGKSTALVGRVLELVTTGTAELRSIAAITFTEKAAAELR
                     DRIRQALERSAERARQDGETQIVERCDDAIDQLDGAAIGTLHAFAQRVLAENPIEVQL
                     PPRVEILDEVTSDVQFEQRWSAYLDHLLEDPAMERTLLLLFATGVKPAALRALALEFD
                     RNWDLVDERVADQAPEPPELQAALAVALRSVDAATEAAVHCTNADDKLLLRLHQVAEY
                     AARLRSTGGELDLLDELSGNGTAKPPSFRAGNIGSKGNWGCDHKAIQADLAAAGEQLA
                     AVAGDVAQACADRIAAALRGFTLDAAAARREAGQLAFHDLLVLARQLVRQHAPVRERL
                     HRRYQRLLLDEFQDTDPIQIEIAVRIAAADPGSDDAGLLAWDEVAVAPGHLFVVGDPK
                     QSIYRFRRADIGLFLAAKDRYGAAGGLVSLSTNFRTGEPIIGWVNQAFAALMAEPPGV
                     DAPIASQPGYVPLHAVRPGPDVGPAVSVIGRDAVEAGSAGELRAAEARQVAATIARIQ
                     AEGWSVGDPSSATGWRDPKLGDITVLVPARTSLPFLEDALDAARIPYRAESSSLVYVS
                     RAVRDLLMALRAVDDPTDHLAIVSALRSPLFACGDDDLFRFKVLQRGHWNFASRQPDT
                     VEPGPVPEALRYLAALHRERHWTSPAELADRIARDRRLFELGFAEGRTRDVWRRLRFV
                     IDQAREWGEATDGNLREYLRWVARQSSEGSRVAEAVLPESDDDAVRIMTIHAAKGLEF
                     PITILSGMSTAPQARPSPAQVVFPPAGGVGYRFGKAVSTQEFEDYKPVDEQMAFDERV
                     RLLYVAATRAKDHLVLSLQRKVRAKAPEPSKRTNAELLLAGCGDGLADLPDAVDADVE
                     APTIAAAPPPPAPAPFAEWRAELDAALAAGSRPTTVAATALTAEGAPDPARDEPEADP
                     GPDPGLIDEHEPVEAGLQKRPRDLDLPPWLKGRYGTAVGRAVHGTLQTIDLADPSGLA
                     GAVAAQCEAE"
     gene            complement(3292..>4696)
                     /locus_tag="VNQ33_09520"
     CDS             complement(3292..>4696)
                     /locus_tag="VNQ33_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWJ98386.1"
                     /translation="GHVDRLHHQFLATIGGAARQVLCVPKGDLRRSRERVPSRWVLDV
                     ASVLAGQTWWSDDLLHRSEPWLTHLASFDDAIRTAAVPATEQEHRLRQLLALDPPRRQ
                     LHAAAGSVDATLASGVDVVDQRWSDRLTRFDGNLAGLPIPSPVDRGASATSLESWATC
                     PHGYFVRHLLGVQPLEQPEDVLQITPLDKGNLVHAALEAFIEEALDQRRVPAVGGRWT
                     DDDRARLAEIAIGLCDEAEADGLTGRSLFWQRDRGKILADLDRFLTEDDALRNASGAV
                     PYATELPFGTGGHPPVAFPLTDGRAVPLRGKADRVDKSDDGRLHVLDYKTGSTRAHGG
                     ISAEDPHQGGRRLQLAVYGLAARQSTGLPHAPVRADYWFTSTKGRWEQLGYEITDEVL
                     SEVSAAVTTIVHGIEGGVFAPHPIPHSTSPFPDCPACDPDNLGTTEVRRHFERKIADP
                     VLADYAALALGTDEEAS"
BASE COUNT          572 a         1567 c         1901 g          656 t
ORIGIN      
        1 actcggcttc gcactgggcg gcgacggcgc cggcgagccc gctgggatcg gcgaggtcga
       61 tggtctggag ggtgccgtgg acggcacggc cgacggcggt gccgtagcgg cccttgagcc
      121 acggcggcag gtccaggtcc cgggggcgct tctggaggcc ggcctcgacg ggttcgtgct
      181 cgtcgatcag accggggtcg gggccggggt cagcctctgg ttcgtcgcgc gccggatccg
      241 gcgcgccttc ggcggtgagg gcggtggccg ccacggtggt gggacgggag ccggcggcga
      301 gggcggcgtc gagctccgct cgccactcgg cgaacggggc cggagccggg ggtggggggg
      361 cggccgcgat cgtcggtgcc tccacgtcgg catcgacggc gtcggggagg tcggccagcc
      421 cgtcgccgca tccggccagg aggagctcgg cgttggtgcg cttcgacggt tcgggagctt
      481 tggcccggac cttgcgctgg agcgagagga ccaggtggtc cttggcccgg gtggcggcga
      541 cgtagaggag gcgcacccgt tcgtcgaagg ccatctgctc gtcgacgggc ttgtagtcct
      601 cgaactcctg ggtggacacg gccttgccga accggtagcc gacgccgccg gccggcggga
      661 acacgacctg ggccggcgac ggccgggcct ggggcgccgt ggacatgccg ctcaggatcg
      721 tgatggggaa ctccaggccc ttggcggcgt ggatggtcat gatccgcaca gcgtcgtcgt
      781 cggactcggg caggacggct tcggccaccc ggctgccctc gctgctctgg cgggcgaccc
      841 accggaggta ctcgcgcagg ttgccgtcgg tggcctcgcc ccactcccgg gcctggtcga
      901 tgacgaaccg gaggcgccgc cacacgtcgc gggtgcggcc ctcagcgaag cccagttcga
      961 agaggcgccg gtcgcgggcg atgcggtccg ccagctcggc gggggaggtc cagtgccgct
     1021 cgcggtggag agcggccagg tacctgaggg cctcgggcac ggggccgggt tcgacggtgt
     1081 cgggctggcg ggaggcgaag ttccagtggc cgcgctggag gaccttgaag cggaagaggt
     1141 cgtcgtcgcc gcaggcgaag agcgggctgc ggagggccga gacgatggcg aggtggtcgg
     1201 tggggtcgtc gaccgcgcgc agtgccatca gcaggtcgcg cactgctcgg gagacgtaca
     1261 cgaggctgct ggactcggcc cgatagggga tgcgggcggc gtcgagcgcg tcctcgagga
     1321 acgggagcga cgtgcgggcc ggcacgagca cggtgatgtc gccgagcttc ggatcgcgcc
     1381 agccggtcgc gctcgacggg tcccccacgc tccagccctc ggcctggatg cgggcgatgg
     1441 tggcagccac ctgacgggcc tcggcagcgc gcagctctcc ggccgagccc gcttcgacgg
     1501 cgtctcggcc gatgaccgac accgcgggcc cgacgtcggg cccggggcgc acggcgtgga
     1561 gcgggacgta gccgggttgc gacgcgatgg gtgcgtcgac gccgggcggc tcggccatga
     1621 gcgcggcgaa cgcctggttg acccagccga tgatcggctc gccggtgcgg aagttggtgg
     1681 agaggctgac gagtccacca gccgcgccgt agcggtcctt cgcagcgagg aacaggccga
     1741 tgtcggcccg gcggaagcgg tagatcgact gcttcggatc gcccaccacg aacaggtggc
     1801 cgggggcgac ggcgacctcg tcccacgcca gcaggccggc gtcgtctgat ccggggtcgg
     1861 cggcggcgat gcgcacggcg atctcgatct ggatcggatc ggtgtcctgg aactcgtcga
     1921 ggaggaggcg ctggtaccgg cggtggaggc gctcgcggac cggcgcgtgc tggcggacga
     1981 gctggcgggc gagcacgagc aggtcgtgga acgcgagctg gcccgcctcc cggcgagcgg
     2041 cggcggcatc gagcgtgaag ccccggagcg cggcagcgat gcggtcggca cacgcctggg
     2101 ccacgtcgcc ggccacggcg gccagttgct cgcccgccgc ggcgaggtcg gcctggatgg
     2161 ccttgtggtc gcacccccag ttgcccttcg agccgatgtt gccggcccgg aagctcgggg
     2221 gcttggcggt gccgttgccg ctcagctcgt cgagcaggtc gagctcgccg ccggtggaac
     2281 ggaggcgggc ggcgtactcg gccacctggt ggaggcggag gagcagcttg tcgtcggcgt
     2341 tggtgcagtg gacggcggct tcggtggcgg catcgaccga gcgcagggcc accgccagcg
     2401 ccgcctgcag ctcgggcggt tccggcgcct ggtccgccac ccgctcgtcg acgaggtccc
     2461 agttgcggtc gaactccagg gcgagggccc ggagcgcggc gggcttcacg cccgtggcga
     2521 acaggagcag gagggtgcgc tccatcgccg ggtcctcgag caggtggtcc aggtaggcag
     2581 accagcgctg ctcgaactgc acgtcagagg tgacctcgtc gaggatctcg acccggggcg
     2641 ggagctggac ctcgatgggg ttctcggcca gcacccgctg ggcgaaggcg tggagggtgc
     2701 cgatggcggc gccgtcgagc tggtcgatgg cgtcgtcgca gcgttcgacg atctgcgtct
     2761 cgccgtcttg tcgggcccgt tcggcgctgc gctccagtgc ctggcggatg cggtcgcgca
     2821 gttcggccgc cgccttctcg gtgaacgtga tggcggcgat gctgcgcagc tcggcggtgc
     2881 cggtggtgac cagctccagc acccggccga cgagggccgt ggacttgccg gacccggcgc
     2941 cggcctcgac gaacagcgtg gtgcccagcg ccttgcgcac gacctcgcgc tggtcctggt
     3001 cggggggcgg cggccgctcg tcgaccagcg ccgccgcagc cgagaccgcc tccgcgccag
     3061 cctcggcctc ggcctcgggc tcggcctccg cctcctccgc ctccgcctcc gcctccgcct
     3121 ccgctccagg ctcgggctcg ggctcggcca cggcctcggg gtcgggctcg gggtcggggt
     3181 cgggctcggc gtttggctct aggccagggg tgggatccga cgtgggatcg gactcgggcg
     3241 ccgacgagcc gccggtctcg tcgaagggct cgtcgaggaa gtcgaagagg ctcacgatgc
     3301 ctcctcgtcg gtgccgaggg cgagggcggc gtagtcggcg aggacgggat cggcgatctt
     3361 gcgctcgaag tggcggcgga cctcggtggt gccgaggttg tcggggtcgc acgcagggca
     3421 gtcggggaac ggcgaggtgc tgtgcgggat cgggtgcggt gcgaacacgc cgccctcgat
     3481 gccgtggacg atcgtggtca cggcggccga cacctcgctc aggacctcgt cggtgatctc
     3541 gtagccgagc tgctcccagc ggcccttcgt ggacgtgaac cagtagtcgg cccggacggg
     3601 cgcgtggggc aggcccgtgc tctggcgcgc ggccaggccg tacaccgcca gctggaggcg
     3661 gcgacctccc tggtgcgggt cctcggcgct gatcccgcca tgggcccgcg tgctgcccgt
     3721 cttgtagtcg aggacgtgga ggcgcccgtc gtcggacttg tcgacgcggt ccgccttgcc
     3781 ccgcagcggc acggctcggc cgtcggtcag ggggaacgcc accggcgggt gcccgccggt
     3841 cccgaacggc agctcggtgg cgtacgggac ggcacccgac gcgttgcgca gcgcgtcgtc
     3901 ctcggtgagg aaccggtcca gatcggccag gatcttcccc cggtcccgct gccagaacag
     3961 cgaccggccc gtgagcccgt cggcctcggc ttcgtcgcac agcccgatgg cgatctcggc
     4021 caggcgagcc cggtcgtcgt cggtccaccg gccaccgacc gcgggaaccc gcctctgatc
     4081 gagcgcctct tcgatgaacg cctccagcgc ggcgtgcacg aggttgccct tgtcgagcgg
     4141 ggtgatctgg aggacgtctt cgggctgttc gagcggctgg acgccgagga ggtggcggac
     4201 gaagtagccg tgcgggcacg tggcccagga ctccaggctg gtggccgacg ctccccggtc
     4261 gaccggcgac gggatgggca ggccggcgag gttcccgtcg aaccgggtca gccggtcgct
     4321 ccaccgctgg tcgaccacgt cgacgcccga cgcgagcgtg gcgtcgaccg agccggcagc
     4381 ggcgtgcaac tggcgccggg gcggatcgag ggcgaggagc tggcgcagcc ggtgctcctg
     4441 ctcggtggcg gggacggcgg cggtgcggat ggcgtcgtcg aacgaggcca ggtgggtgag
     4501 ccacggctcg gaccggtgga gcaggtcgtc gctccaccag gtctggccgg cgagcaccga
     4561 cgccacgtcg agcacccagc gagacggcac ccgctcgcgg ctgcggcgca ggtctccctt
     4621 gggcacgcac agcacctgcc gggccgcgcc gccgatggtg gcgaggaact ggtggtgcag
     4681 ccgatcgacg tggccg
//
LOCUS       DATMFS010000504         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_41149, whole genome shotgun sequence.
ACCESSION   DATMFS010000504 DATMFS010000000
VERSION     DATMFS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41149"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VNQ33_09525"
     CDS             <1..1572
                     /locus_tag="VNQ33_09525"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWJ98387.1"
                     /translation="LGDNMLITFLGWEGVGACSYFLISFWHTDDANASAGKKAFVTNR
                     IGDFGFMVAIFFTFAALGTINYTGIVAASPSLATTTATVIAAMLFLGACGKSAQLPLY
                     VWLPDAMAGPTPVSALIHAATMVTGGIYLLARMNPVIVEASSWVPWMIAVVGALTALF
                     AATIAVAQNDIKKVLAYSTVSQLGFMFLAIGSGAYVAALFHMITHAFFKGLLFLGSGS
                     VIHGMEGEQDMRRMGGLFKKMPITAVTFMVGWLAIAGFPFFAGFFSKDEILLFAYDKS
                     IVLWLIGMVAAGLTGFYMTRQVIMVFFGAPRWDRPLEEAAPELFAEREAAGIHPGHGA
                     GPTGEIHPHESPWTMTLPLIVLGPLALLGGWLNSAFWEKGKALEHWLEPVIEFGEHHL
                     SAGTTTLLKIELGIGATLVGLIGMTAAWFVYSRNKGEAKDRIELKVLLKGWYYDSSIA
                     AFMGGPGRKGFDAIAWFDKHVIDGAVNGVGSVVKVTSDKGRTVQTGYVRNYALGLLVG
                     ALVVAALILSKAVTG"
     gene            1569..3236
                     /locus_tag="VNQ33_09530"
     CDS             1569..3236
                     /locus_tag="VNQ33_09530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWJ98388.1"
                     /translation="MNPLLLASEGGHATAANPSWPLLSFLVLTPVFGALVVALISKRR
                     PELTKQAAVLFSLVTGALAVGVVVLFDKHVPGFQLVDDVQWLKNPDISWKLGVDGISL
                     WLVALTGLLFPIAMLGAPPHGDDKPFYAWLLVLEAGCLGAFLALDTFLFFVFFEIVLV
                     PMYFLISGWGYGERKYAATKFFLYTMFGSAFMLVGTVALSYLHAKGGPVTFDLQVLAD
                     HQGVLAQSTARWLFVSFAIAFAVKVPIFPVHTWLPDAHTQAPTAGSVILAGVLLKLGT
                     YGFLRYGLYLFPEASAWAAPGMVTLGVIGIIYGAVCATMQKDLKRLVAYSSVAHMGFI
                     VLGIFAITRQALNGSVLQMVNHGVSTGALFLLVGFIYERRHTREIAELKGLQKPAPKM
                     AAVFTLVMLSSIGLPGLNGFVGEFLILIGSFLTVRWWAVVAVTGVILAALYLLWAYQR
                     VFHGEPDGDNKTFKDITFTEGLVMAPLLLLIVFMGLFPKPFLDRIEPSVDKLVHHVEA
                     NSDHQEPAVSTKGDEIVPESERRSEAEAAAEGEHGGAEGEHRTEEGN"
     gene            3236..>3519
                     /locus_tag="VNQ33_09535"
     CDS             3236..>3519
                     /locus_tag="VNQ33_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98389.1"
                     /translation="MLAEVERLSTPQVEWSVLLPFQILLGGAVILMVAGALTPRRTKA
                     PWHAGATVAIAVASMVSTVFLWWRVRDDGPISAVSNAVVVDGLSLYLGFA"
BASE COUNT          476 a         1296 c         1093 g          654 t
ORIGIN      
        1 ctgggcgaca acatgctcat caccttcctc ggctgggagg gcgtgggcgc ctgctcgtac
       61 ttcctcatct ccttctggca caccgacgac gccaacgcct cggccggcaa gaaggcgttc
      121 gtcaccaacc gcatcggcga cttcggcttc atggtggcga tcttcttcac cttcgccgcc
      181 ctcggcacca tcaactacac gggcatcgtc gccgcctcgc ccagcctggc caccaccacc
      241 gccacggtca tcgccgccat gctcttcctc ggggcctgcg gcaagtcggc ccagctgccg
      301 ctctacgtct ggctgcccga cgccatggcc ggcccgacgc cggtctcggc cctcatccac
      361 gccgccacca tggtcaccgg cggcatctac ctcctggccc gcatgaaccc ggtcatcgtc
      421 gaggcatcca gctgggtgcc gtggatgatc gccgtcgtcg gtgccctcac cgccctgttc
      481 gccgccacca tcgccgtcgc ccagaacgac atcaagaagg tgctggcgta ctccaccgtc
      541 agccagctcg gcttcatgtt cctggccatc ggctcaggcg cctacgtggc cgccctgttc
      601 cacatgatca cccacgcctt cttcaagggc ctcctgttcc tcggctcggg ctcggtgatc
      661 cacggcatgg agggcgagca ggacatgcgc cgcatgggcg ggctgttcaa gaagatgccc
      721 atcaccgcgg tgacgttcat ggtcggctgg ctcgccatcg ccggcttccc gttcttcgcc
      781 gggttcttct cgaaggacga gatcctgctc ttcgcctacg acaagagcat cgtgctgtgg
      841 ctcatcggca tggtcgccgc ggggctcacc ggcttctaca tgacccgcca ggtgatcatg
      901 gtcttcttcg gcgccccgcg ctgggatcgc ccgctcgagg aggcggcgcc tgagctcttc
      961 gccgagcgcg aggcggctgg catccacccc ggtcacggcg ccggtcccac cggtgagatc
     1021 cacccgcacg agtccccgtg gaccatgacg ctgccgctca tcgtcttggg cccgctcgcc
     1081 ctgctgggcg gctggctcaa ctccgccttc tgggagaagg gcaaggccct cgagcactgg
     1141 ctggagcccg tgatcgagtt cggcgagcac cacctcagcg ccggcaccac caccctgctc
     1201 aagatcgagc tgggcatcgg cgccacgctg gtcgggctca tcggcatgac ggctgcctgg
     1261 ttcgtgtact cccgcaacaa gggcgaggcg aaggaccgca tcgagctcaa ggtcctgctc
     1321 aagggctggt actacgactc gtcgatcgcc gcgttcatgg gcggcccggg ccgcaagggc
     1381 ttcgacgcca tcgcctggtt cgacaagcac gtcatcgacg gggccgtgaa cggcgtgggc
     1441 tcggtggtga aggtgacctc ggacaagggc cgcacggtcc agaccggcta cgtgcgcaac
     1501 tacgcgctcg gcctcctcgt cggcgccctc gtggtcgccg ccctcatctt gtcgaaggcg
     1561 gtgaccggat gaacccgctc ctgctcgcct ccgagggcgg ccacgccacc gcggccaacc
     1621 cctcctggcc cctgctctcg ttcctggtcc tgacgccggt gttcggcgcc ctcgtggtgg
     1681 cgctgatctc caagcgccgc ccggagctca ccaagcaggc cgcggtcctc ttctcgctgg
     1741 tcaccggggc cctcgccgtc ggcgtggtcg tgctgttcga caagcacgtg cccggcttcc
     1801 agctggtcga cgacgtccag tggctgaaga accccgacat ctcgtggaag ctcggcgtcg
     1861 acggcatctc gctgtggctc gtcgcgctca ccggcctcct gttcccgatc gccatgctcg
     1921 gtgcgccgcc gcacggcgac gacaagccgt tctacgcctg gctgctcgtg ctcgaggccg
     1981 gctgcctcgg agcgttcctg gcgctcgaca cgttcctctt cttcgtgttc ttcgagatcg
     2041 tcttggtccc gatgtacttc ctgatcagcg ggtggggcta cggcgagcgg aagtacgccg
     2101 ccaccaagtt cttcctgtac acgatgttcg gctcggcctt catgctggtc ggcaccgtcg
     2161 ccctctcgta cctgcacgcc aagggcgggc cggtcacctt cgacctccag gtcttggcgg
     2221 accaccaggg cgtcttggcg cagagcacgg cccgctggct gttcgtctcc ttcgccatcg
     2281 ccttcgccgt gaaggtcccg atcttcccgg tgcacacctg gctgcccgac gcccacaccc
     2341 aggcgcccac cgccggctcg gtgatccttg ccggcgtgct gttgaagctc ggcacctacg
     2401 ggttcttgcg ctacggcctc tacctgttcc ccgaggcgtc ggcctgggcg gcgccgggga
     2461 tggtcacgct cggcgtcatc ggcatcatct acggcgccgt gtgcgccacg atgcaaaagg
     2521 acctcaagcg cctcgtcgcc tactcgtcgg tggcccacat gggcttcatc gtgctgggca
     2581 tcttcgccat cacccgccag gccctgaacg gctcggtgct gcagatggtc aaccacggcg
     2641 tctccaccgg cgccctgttc ctcctcgtcg gcttcatcta cgagcgccgg cacacccggg
     2701 agatcgccga gctgaagggc ctccagaagc cggctccgaa gatggccgcc gtcttcaccc
     2761 tggtgatgct gtcgtccatc ggcctccccg gcctgaacgg cttcgtcggc gagttcctga
     2821 tcttgatcgg ctcgttcctc accgtccggt ggtgggccgt cgtggccgtc accggcgtga
     2881 tcctcgccgc cctgtacctg ctctgggcct accagcgggt cttccacggc gagcctgacg
     2941 gcgacaacaa gaccttcaag gacatcacgt tcaccgaggg cctggtcatg gccccgctgc
     3001 tcttgctcat cgtcttcatg ggcctgttcc cgaagccgtt cctcgatcgc atcgagccgt
     3061 cggtcgacaa gctggtccac cacgtcgagg ccaactcgga tcaccaggag cccgccgtct
     3121 cgacgaaggg cgacgagatc gtgcccgagt ccgagcgccg gtctgaggcg gaagccgccg
     3181 cggaaggcga gcacggtggg gccgagggcg agcaccgcac cgaggaaggc aactgatgct
     3241 ggccgaagtg gagcgcttgt cgaccccgca ggtcgagtgg tcggtcctgc tgccgttcca
     3301 gatcctgctg ggcggcgccg tgatcttgat ggtggcgggc gcgctgaccc cccgtcgcac
     3361 caaggcgccg tggcacgccg gggccaccgt cgccatcgcc gtggcgtcga tggtctccac
     3421 ggtcttcctg tggtggcggg tccgtgacga cggccccatc tcggccgtgt cgaacgccgt
     3481 ggtcgtcgac ggcctgagcc tctacctggg cttcgccat
//
LOCUS       DATMFS010000505         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_63033, whole genome shotgun sequence.
ACCESSION   DATMFS010000505 DATMFS010000000
VERSION     DATMFS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63033"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VNQ33_09540"
     CDS             complement(<1..571)
                     /locus_tag="VNQ33_09540"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HWJ98390.1"
                     /translation="MNKRKPGSANFAIDEPPLKLVLVEGEGGGTLNHLGVEVDMAEEF
                     VAAEARLTDDGLDTTGVDDTTCCYALKTETWVNDPDGAPWEWYVKTGDIEQMGTTAAD
                     AEGGACCSPAAISESVTLGRGPGCGRRLRTCRAAPARRRVRRGLVGSSPGSGPSRGPA
                     RGARRRGDGREQPGLTSAPGPAGPCGRSRT"
     gene            complement(774..920)
                     /locus_tag="VNQ33_09545"
     CDS             complement(774..920)
                     /locus_tag="VNQ33_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98391.1"
                     /translation="MGGEPCVVLLDFGQPTIDPGSQRLSDVAIDAADDAVGADLENQL
                     FPWP"
     gene            complement(1026..>2356)
                     /gene="dnaB"
                     /locus_tag="VNQ33_09550"
     CDS             complement(1026..>2356)
                     /gene="dnaB"
                     /locus_tag="VNQ33_09550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWJ98392.1"
                     /translation="SSNGGRVPPHNLAAEESLLGAMLLSRTAIDIASESVAPSDFYKP
                     AHGHIYEAITSLSARGEPVDPVTVADELSRHDLLDVIGGPPALINLQSATPATSSASR
                     YAKIVEEHSLLRGLIGVAGEIAEMGYDIPDDVPKAIDRAETLVFEIAQHRQTDSMSRL
                     HDLLDQTVDNLEALYDRGDSITGTPTGYYDFDELTSGLQPNALIVIGARPGMGKTSFA
                     MGMAGHAAIEEQRPVLFFSLEMSRVELSQRLLCSEARVDSTKVRNGRLTDDDWSRISH
                     AIGRMGEAPLWIDDNPNVTVMEIRAKARRLQSRVGTLGMIVVDYIQLMTGNARAENRQ
                     TEVAEISRGLKILARELQCPVVALAQLNRGLEQRADKRPMLSDLRESGSLEQDADIVT
                     FLYRDEHYNPDSADRGTAEVLVAKHRAGPTGMIRLAFLDHYTKFANMARE"
BASE COUNT          342 a          780 c          862 g          372 t
ORIGIN      
        1 cggtccggct gcgaccgcac ggccccgccg gtccaggcgc cgacgtgaga ccaggctgct
       61 cccttccgtc gcccctcctg cgcgctccgc gggcggggcc gcgtgagggc cctgaccccg
      121 gggacgaccc gacgagtcct cgtctgactc gacgacgtgc aggagccgct cggcaagtcc
      181 gcagccgacg accgcacccg gggccacggc cgagggtcac cgactcgctg atggccgccg
      241 gcgagcagca ggcgccgcct tcggcgtcgg cagccgtggt gcccatctgc tcgatgtcac
      301 ccgtcttcac gtaccactcc cacggcgcgc cgtcggggtc gttgacccag gtctcggtct
      361 tgagggcgta gcagcaggtg gtgtcgtcga ccccggtggt gtccaggccg tcgtcggtca
      421 accgggcttc ggcggcgacg aactcctcgg ccatgtcgac ctcgaccccc aggtggttga
      481 gcgtcccgcc gccctccccc tcgaccagca ccagcttgag cggcggctcg tcgatggcga
      541 agttggcgga gccaggcttg cgcttgttca cctcggtgcc gaacagactg ctacccgtcg
      601 atcgtcggag cacgtggccc ctgcgccatc cgaccgacca cggcatcccg acccgatgca
      661 gagcacgagc acgacgaacc ccaggaccct tcgtcgaggc tcgccgacga acttctcgaa
      721 cccgccgaag ccatgcccga agcgtcgagc ggaacgtcgt ggcgtgatgg gtctcatggc
      781 caagggaaga gctggttctc gagatcggca ccgacggcat cgtcggccgc gtcgatcgcg
      841 acgtcggaga gccgctggct accgggatcg atcgtcggct ggccgaagtc gaggagcacg
      901 acgcacggtt cgccgcccat cggatcccat cggccaagcg cggatgacca tgagaaggga
      961 ccgggaggcc ggcgtgccct gcatgtgccg ttcaggggca cccgaagggc gggtggcgca
     1021 cggggctatt cgcgggccat gttggcgaac ttggtgtagt ggtccaagaa cgcgaggcgg
     1081 atcatgccgg tgggtccggc acggtgcttg gcgacgagca cctcggccgt gccgcggtcg
     1141 gcggagtcgg ggttgtagtg ctcgtcccgg tacaggaacg tcacgatgtc cgcatcctgc
     1201 tcgagcgatc cactttcacg gaggtcggag agcatggggc gcttgtcggc ccgttgctcg
     1261 aggccgcggt tgagctgggc gagggcgacg accgggcact ggagctcacg ggcgaggatc
     1321 ttgaggcccc ggctgatctc ggccacctcg gtctggcggt tctcggcacg ggcgttgccg
     1381 gtcatgagct ggatgtagtc caccacgatc atgccgagcg tcccgacccg ggactggagg
     1441 cgacgagcct tggcccggat ctccatgacc gtgacgttgg ggttgtcgtc gatccacagc
     1501 ggcgcctcgc ccatgcggcc gatggcgtgg ctgatgcggc tccagtcgtc gtcggtgagg
     1561 cggccgttgc ggaccttcgt ggagtccacc cgggcctccg agcacaacag gcgctgcgac
     1621 agctcgaccc ggctcatctc cagcgagaag aacaggacgg gccgctgctc ctcgatggcg
     1681 gcgtggccgg ccatgcccat ggcgaacgag gtcttgccca tgccgggccg ggcgccgatg
     1741 acgatgaggg cgttgggctg caagccggac gtgagctcgt cgaagtcgta gtacccggtg
     1801 ggtgtgccgg tgatggagtc gccccggtcg tagagggcct cgaggttgtc gacggtctgg
     1861 tccagcaggt cgtgtagccg cgacatggag tcggtctggc ggtgctgggc gatctcgaag
     1921 acgagggtct cggcccggtc gatggccttg ggcacgtcgt cggggatgtc gtagcccatc
     1981 tcggcgatct cgccggcgac gccgatgagg ccgcgcagca gcgagtgctc ctcgacgatc
     2041 ttggcgtagc gcgacgcgct ggaggtggcc ggcgtggccg actgcaggtt gatcagcgcc
     2101 ggggggccgc cgatgacgtc gagcaggtcg tggcgggaca gctcgtcggc cacggtgacc
     2161 gggtcgacgg gctcaccccg cgccgacaac gacgtgatgg cctcgtagat gtggccgtgc
     2221 gccggcttgt agaagtccga cggcgccacc gactccgagg cgatgtcgat cgccgtgcgt
     2281 gagagcaaca tggcgcccag cagggactcc tctgccgcca ggttgtgcgg cggcacgcga
     2341 ccgccgttgg aggacc
//
LOCUS       DATMFS010000506         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_203546, whole genome shotgun sequence.
ACCESSION   DATMFS010000506 DATMFS010000000
VERSION     DATMFS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203546"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VNQ33_09555"
     CDS             complement(<1..332)
                     /locus_tag="VNQ33_09555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ98393.1"
                     /translation="MGADHRARWAHRPGLDGVRGLAVVAVVAYHLGYLHGGFLGVDVF
                     LVLSGFLITGLALGEIGETGHLSLRAFWGRRVRRLVPALLVVVLVVTLVALAIGWPRD
                     QLDSLGWDG"
     gene            complement(401..1645)
                     /locus_tag="VNQ33_09560"
     CDS             complement(401..1645)
                     /locus_tag="VNQ33_09560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWJ98394.1"
                     /translation="MTIQRTDLPSGLTVVTEAMAGSRSASFGCWVRVGARDEGDDLAG
                     ASHFLEHLLFKGTETRSGREISEAIEAVGGELNAHTAHEHTAFYARVPAEAAAVGLEL
                     LCDVVTSPMFDVDDVESERQVILEELHLAEDEGDDRVMSLAHEALWGDHPLGREVLGT
                     LATIDGMDRGAIAAFFEHHYHPANLVLVAAGAVDHEAVVAAGERFPDRSPGERPAREA
                     PGGPIPVRERMLLRRPSEQAHLCAVWPALASDDPDRYALGVANQILGGGLSSRLFQEI
                     REERGLAYTVFSSVAAFSDAGSLAAYVGTGAERVDEVRKLLLEITAELVEGGVTEREW
                     EVARGYLEGSFLLGLEDSGSVMARLGNHVCSRGRVTPVEDQLAKLRAVTIEQVGAAAQ
                     RVLAMPPTICGVGPLTEADLGG"
     gene            complement(1770..>2794)
                     /locus_tag="VNQ33_09565"
     CDS             complement(1770..>2794)
                     /locus_tag="VNQ33_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWJ98395.1"
                     /translation="ASVCASSLSLMDAGVPLRAPVAGIAMGLVFAEGKYTPLTDILGA
                     EDAYGDMDFKVAGTSEFVTALQLDTKIEGLPSEVLAAALEQAKVARLEILDVMNAAIP
                     APRESVGASAPKIISFEIPIDKIGEIIGPKGKVINAMQAETGADISVDDDGMVGTVSI
                     GSSDMGAVQEAERQIRLILNPPSAEVGATYPGRVVNITKFGAFVNILPGRDGLVHISK
                     MGGGKRIEKVEDVLELGQELLVKVDDVDPNGKVSLTPVTPLVPGGGSGGDAPAPSGSD
                     DAPASDGGSAPKRETVSFEEAFDAEVREEFGDLGPGSERPGGSGGGRSGGRGGRDGGR
                     RGGGRR"
BASE COUNT          420 a         1013 c         1006 g          355 t
ORIGIN      
        1 ccgtcccagc cgagcgagtc gagctgatcg cgcggccagc cgatggcgag ggcgacgagc
       61 gtgacgacga ggaccaccac cagcagggcc gggaccaacc gccggacccg tcggccccag
      121 aacgcacgca gcgacaggtg ccccgtctcc ccgatctccc ccagcgccag gccggtgatc
      181 aagaagcccg agaggaccag gaacacgtcg acgccgagga agccgccgtg caggtagccg
      241 aggtggtagg ccacgacggc cacgaccgcg aggccgcgca cgccgtcgag cccgggccgg
      301 tgggcccagc gcgctcggtg gtcagccccc atgagggacc aaccctacgg gcggacccgc
      361 gcccgcaccc ttcgatccgg gacgccctgg ccccgcaccg tcacccgccg aggtcggcct
      421 cggtcagcgg acccacgccg cagatcgtcg gcggcatggc cagtacccgc tgggccgctg
      481 cgccgacctg ctcgatggtg acggctcgca gcttggccag ttggtcctcg accggggtca
      541 cccggccgcg ggagcacacg tggttgccga gccgggccat cacgctgccg ctgtcctcca
      601 agccgagcag gaacgatccc tccaagtacc cgcgggccac ctcccactcg cgctcggtga
      661 cgccgccctc caccagctcc gcggtgatct cgaggagcag cttgcggacc tcgtcgaccc
      721 gctcggcccc cgtgccgacg tacgcggcga gcgatccggc gtcagagaac gccgccaccg
      781 aggagaacac ggtgtaggcc aggccccgct cctcgcggat ctcctggaac aaccggctgg
      841 acaggccccc gccgaggatc tggttggcca cgccgagcgc gtaccggtca gggtcatcgg
      901 acgcgagggc gggccagacg gcgcagaggt gggcctgctc ggacggccgg cgcaggagca
      961 tccgctcgcg caccgggatc gggccgcccg gtgcctcgcg cgcgggccgc tctcctggtg
     1021 aacgatccgg gaaccgctcg ccggccgcga ccacggcctc gtggtcgacc gcaccggcgg
     1081 cgacgaggac gaggttcgcc gggtggtagt ggtgctcgaa gaaggcggcg atggcgccgc
     1141 ggtccatccc gtcgatcgtg gcgagcgtgc cgaggacctc ccggccgagc gggtggtcgc
     1201 cccagagcgc ttcgtgggcg agggacatga cccggtcgtc gccctcgtcc tcggccaggt
     1261 gcagctcctc gaggatgacc tggcgctcgg actcgacgtc gtcgacgtcg aacatcggcg
     1321 acgtcacgac atcgcacagc agctcgaggc ccaccgccgc cgcctccgcc gggacccggg
     1381 cgtagaaggc cgtgtgctcg tgggccgtgt gggcgttgag ctcgccgccc acggcctcga
     1441 tggcctcgga gatctcccgg cccgagcggg tctcggtgcc cttgaacagc aggtgctcca
     1501 ggaagtgcga agcaccggcg aggtcgtcgc cctcgtcacg ggccccgacc cgcacccagc
     1561 agccgaagct cgccgagcgc gagccggcca tggcctcggt gacgacggtg aggccagagg
     1621 ggaggtcggt gcgctggatg gtcacaggtg ctcgctcgat cgaaggcagg aggggaaaac
     1681 gccgtcggtc ccagccagag gccgggaccg acgggtacta cggaacgtgc aggtcaggca
     1741 cggcggcccg accctctggc acggtcagct cagcgacgac cgccgccacg gcgaccgccg
     1801 tcgcgaccgc cacggccgcc cgagcgaccg ccgccgctgc caccgggacg ctcggaaccg
     1861 ggaccgaggt caccgaactc ctcgcgcacc tcggcgtcga aggcctcctc gaaggacacg
     1921 gtctcgcgct tgggggccga gccgccgtcg gacgccggcg cgtcgtcgga gccggacggg
     1981 gccggagcgt cgccgcccga gccgccgccg gggaccagcg gggtgaccgg ggtgagcgac
     2041 accttgccgt tggggtcgac gtcgtcgacc ttgaccagca gctcctgacc gagctcgagg
     2101 acatcctcga ccttctcgat gcgcttgccg ccgcccatct tggagatgtg gacgaggccg
     2161 tcgcggcccg ggaggatgtt cacgaacgca ccgaacttgg tgatgttcac gacccggccc
     2221 gggtaggtgg cgccgacctc ggccgacggc gggttgagga tgaggcggat ctggcgctcg
     2281 gcctcctgca ccgcacccat gtcgctggag ccgatgctca cggtgccgac catgccgtcg
     2341 tcgtcgacgg agatgtcggc gccggtctcg gcctgcatgg cgttgatgac cttgcccttc
     2401 gggccgatga tctcaccgat cttgtcgatc gggatctcga agctgatgat cttcggggcc
     2461 gaggcgccga cggactcacg gggcgccggg atggcggcgt tcatcacgtc gaggatctcg
     2521 aggcgggcga ccttggcctg ctccagggcg gccgccagga cctcggaggg caggccctcg
     2581 atcttggtgt cgagctggag ggcggtcacg aactcgctcg tgcccgccac cttgaagtcc
     2641 atgtcgccgt aggcgtcctc ggctccgagg atgtcggtga gcggcgtgta cttgccctcg
     2701 gcgaacacga gccccatggc gatgccggcc accggggcac gcaggggcac gccggcgtcc
     2761 atcagggaca gcgacgaggc gcacaccgag gcca
//
LOCUS       DATMFS010000507         5471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_343550, whole genome shotgun sequence.
ACCESSION   DATMFS010000507 DATMFS010000000
VERSION     DATMFS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343550"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VNQ33_09570"
     CDS             complement(<1..632)
                     /locus_tag="VNQ33_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWJ98396.1"
                     /translation="MSEPDVAIPRRGASSGPSALRRRLGLLGGIGLALLGSVLILRPF
                     ASLSILVVLVTTGLLVGGVLTLVRSAAEHRPWVARALGTVWLVAGVVAVVWPQLTTRG
                     IAVIAGLSMVVGGVVDVLGGLAGTKDERAASVIWGLAGTVLGVLALVWPDVTLLVVAV
                     VFGMRILVLGGRLALASVRGSQVSVHDDGDRAKGRVRRLAHVAGVSVVLVL"
     gene            842..2098
                     /locus_tag="VNQ33_09575"
     CDS             842..2098
                     /locus_tag="VNQ33_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HWJ98397.1"
                     /translation="MATPSDPGRPSLRDAYVYLLGRALVARQQATDLAEPGAAFNQLK
                     HNPVATPLEWVNPNLDVTNTEAWIGVDEDTPALLEVPRVDGRYFTVQICDEWGEVITN
                     INERNYPEHRHGTFALVAPGSRAAVPDGAVRIELRSPKAKLLARIELGSDRDGAVELQ
                     HQISLRSSGSPRVPAPVELPPFDNADLIGVELFEHAEALLTSAPDVSPVAAQFQAKVR
                     DVVASIEDPEERTRTDEALRSTVVPEFQRYAVQEAGAVGGNWLATTVIGNYGDDIAIR
                     TAANYVGIWANSRHEVVYFVTTRDADGNPLDGSLTYVLDFPAASHPDRVVDAYWSLSL
                     VDVPRFVAVENRLDRYTFNSVNPPPANPDGGMQIFVAPDARGTIAEANWLPAAEGRPF
                     SLTFRAYVPREIVKQGEWFPPAPRKV"
     gene            complement(2149..4161)
                     /locus_tag="VNQ33_09580"
     CDS             complement(2149..4161)
                     /locus_tag="VNQ33_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWJ98398.1"
                     /translation="MCEPTPSLDSHTKFVGGQVGAGEVGAIANQLDIVDAGAPLSVPA
                     QTMEGVALDRDLVMYTTSEGGGGDASHPQLLVWAPAASPLARLAVTEVAPWGSDASYG
                     ADWFELTNTGATTLDLDGVKVDDSSNAFGSAAALTGVSSLASGESAIFFEKSTSGDAA
                     AIAAAFGQAWFGDAGLPDGFKIGSYSGSGLGLSSGGDAVNLFAPGTHVTGVSFAGSTA
                     DVTFDNTARLGAVAAPVGIKTLAGEGVFGAFVGADGIGLGSPGNVAPADPGGPGGDTS
                     DIEITEVSAFGSGSAPYAADWIEVTNTGTESVNLAGWKMDDSSALFSAAVDLVGVPVL
                     PAGASAVFFEGTDDAAFQLAFAQAWFGQDLFDSGFLFGHYTGGGVGLSTGGDGVVLFA
                     PDGTLVTGVSFGASPSAAPFATFDNVGGLGASTAPFPVLETLSAVDVDGAFLAVDGHA
                     IGSPGTTTSEEEPSSTEDVVRGLYDAVLRRQLDPAGLSYWAGRIDGGLPPAEVAASLA
                     RSREGWTRVVQQYDRLALDREGDPSGVTYWTNKLQASKAPDVLLADLFGSAEADRRGG
                     STNAGYVSYVSSRVLARAPDSGGLAYWTSKVDHAADPAAARRQAARTILFSPEGLRRQ
                     VHANHQTVCGAPAPGASLGGWTDSFRASRLNPSLLRAVIVIEGCEV"
     gene            complement(4225..>5471)
                     /locus_tag="VNQ33_09585"
     CDS             complement(4225..>5471)
                     /locus_tag="VNQ33_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ98399.1"
                     /translation="DLDEAARLLGLSRWRRFWKLDVPSGAIGLVWNGMMSMGGGWFFL
                     VASESISVLNRSYALPGIGSYVGAAITEGSLRKVAIGAVAMALMVLGVNFMFWRPLVA
                     WAERFKNEQSESEQVPRSMVLDFLRRSHWPRAVGRARRRLAEPLNRAGDRVFGHERPV
                     DADTGTRRRGDLVFAAVTVGVIGYGTVELLRYVTAVDGWGVFVPPLWQGLLTLARVVA
                     VVALSTVVWVPIGVAIGLHPRASRIAQPIVQVLASFPANFLFPFAVWAFVRTGLTLDV
                     GGIVLMALGAQWYILFNTIAGAQSIPGDLIEAMDDLGIGGWLRWKRLILPAVFPSYVT
                     GGITASGGAWNAAIVAEIVTYGSTTLTATGLGAFIAHATQTGDFHRILAGVTVMSLYI
                     IAVNRLFWQRLHRLAEQRYALA"
BASE COUNT          902 a         1946 c         1942 g          681 t
ORIGIN      
        1 aggacgagca ccaccgacac gccggccacg tgggccagtc ggcggacccg ccccttggcc
       61 cgatccccat cgtcgtgcac cgagacctgc gaccctcgga ccgaggccaa cgccagccga
      121 ccgccgagca ccaggatgcg catcccgaac accaccgcga ccacgagcag cgtgacatcg
      181 ggccacacca gcgccaggac acccaagacg gtgccggcca agccccagat caccgaggcg
      241 gcacgctcgt ccttcgtgcc ggccagcccg ccgagcacgt ccacgacgcc gcccacgacc
      301 atcgacaagc cggcgatcac cgcgatgccg cgcgtggtca gttgcggcca caccacagcc
      361 acgacgccgg cgaccagcca gaccgtgccc agggctcgag caacccacgg ccggtgctcc
      421 gcagcggaac ggacgagggt gaggacgcct ccgacgagca gaccggtcgt cacgaggacg
      481 acgaggatcg acagcgacgc aaacggccgc aggatcagga ccgagccgag cagcgccagc
      541 ccgatcccac cgaggagccc gagccggcgc cgaagcgcgc tcgggccgct cgatgcccca
      601 cggcggggaa tcgccacatc cggctcgctc atgccagccc ccggcgcgcc cgctccgacc
      661 cggaccgagc acgcgcgccg cttcgctccc cgtccgccag cttcgcgctc gtggcccgtc
      721 gagacacgcc ccatcgtctc acgactgctc gaccggcgcg ctgaccgaga ccggggaacc
      781 cggtgccggc gaccggctac gcacatgcgt gcgtgggtgc gtgcgtgcca ggatcgcgtc
      841 cgtggcaacg ccgagcgatc caggccgtcc ctccctgcgc gacgcctacg tctacctctt
      901 gggacgggcg ctcgtcgccc ggcagcaggc caccgacctg gccgagccgg gcgcggcgtt
      961 caaccagctc aagcacaacc ctgtcgcaac gcccctcgag tgggtgaacc cgaacctgga
     1021 cgtgaccaac accgaggcct ggatcggcgt cgatgaggac acgccggccc tcttggaggt
     1081 gccgcgggtg gacggccgct acttcaccgt gcagatctgc gacgagtggg gcgaggtcat
     1141 caccaacatc aacgaacgga actatcccga acaccgtcac ggcaccttcg cgctcgtcgc
     1201 cccgggatcg cgggcagcgg tccccgacgg cgcggtgcgg atcgaactgc ggtcgccgaa
     1261 ggcgaagctg ctggcccgca tcgagctcgg ttccgatcgg gacggagccg tcgagctcca
     1321 gcaccagatc tcgctccggt catcggggtc accgcgggtg cccgcgccag tggagctgcc
     1381 gccgttcgac aacgccgatc tgatcggggt ggagctcttc gagcacgccg aggcgttgct
     1441 gaccagcgcg ccagacgtgt cgcccgtcgc cgcccagttc caggccaagg tgcgtgacgt
     1501 ggtcgcttcg atcgaagacc ccgaggagcg tacgaggacc gacgaggccc tgcggtcgac
     1561 ggtcgttccc gagttccagc ggtacgcggt ccaagaggcc ggcgccgtgg gcggcaactg
     1621 gctcgccacc acggtgatcg gcaactacgg ggacgacatc gcgatccgga ccgccgccaa
     1681 ctacgtgggg atctgggcca actcccgcca cgaggtggtc tacttcgtca ccacccgcga
     1741 tgccgacggg aaccccctcg atggatccct cacctacgtg ctcgacttcc ctgcagcgtc
     1801 gcatcccgac cgcgtcgtcg acgcgtactg gtcgctgagc ctggtcgacg ttccccgatt
     1861 cgttgccgtc gagaaccggc tggatcgcta cacgttcaac agcgtcaacc cgccgccggc
     1921 gaaccccgat ggtgggatgc agatcttcgt ggcgccggac gcccgcggca cgatcgccga
     1981 agcgaactgg ctgccagcgg ccgagggccg accgttctcc ctgacgttcc gggcgtacgt
     2041 gccgcgggag atcgtcaagc aaggcgagtg gtttccgccc gccccccgca aggtctgacc
     2101 atcgatgacg aactccgccg cgccgtcgag gagacggtcg gttccgacct agacctcgca
     2161 accctcgatc acgatgaccg cccgtaggag cgaggggttg agacgtgagg ctcggaacga
     2221 atcggtccag ccgcccagcg acgcgccggg cgcgggcgca ccgcacacgg tctggtggtt
     2281 ggcgtggacc tgccgccgca gcccctccgg cgagaagagg atcgtccggg ccgcctgccg
     2341 ccgagcggcc gccggatccg cggcgtggtc gaccttgctc gtccagtagg cgagcccgcc
     2401 gctgtcgggg gcccgagcca ggacccggct ggagacgtag gagacgtagc cggcgttggt
     2461 gctcccgcct cgccggtcgg cctctgcgga gccgaacagg tcagccagca gcacgtcggg
     2521 ggccttggag gcctggagct tgttcgtcca gtaggtcacg ccggagggat cgccctcacg
     2581 atcgagggcg aggcggtcgt actgctgcac gacgcgggtc cagccctccc gtgaccgggc
     2641 gagcgacgcg gcgacctcgg ccggtggcag cccgccgtcg atccgtcccg cccagtagct
     2701 cagcccggcc gggtcgagct ggcggcgcag cacggcgtcg tagagccccc ggaccacgtc
     2761 ctcggtggac gacggctcct cctcggaggt cgtcgtgccg ggcgacccga tggcgtggcc
     2821 gtcgaccgcg aggaacgccc cgtcgacgtc gacggcgctc agcgtctcca gcacggggaa
     2881 gggagccgtg ctggcgccga gtcctccgac gttgtcgaag gtggcgaagg gcgccgccga
     2941 cggcgaagcc ccgaagctca cgccggtgac gagcgtgccg tccggggcga agagcaccac
     3001 gccatcgcca ccggtcgaca ggcccacacc gcccccggtg tagtggccga agaggaagcc
     3061 gctgtcgaag aggtcctggc cgaaccacgc ctgggcgaag gcgagctgga aggcggcgtc
     3121 gtcggtgccc tcgaagaaga ccgccgaggc accggccggg agcaccggca cgccgacgag
     3181 gtcgaccgca gcggagaaga gggccgagga gtcgtccatc ttccagccgg cgaggttcac
     3241 cgactcggtg ccggtgttgg tgacctcgat ccagtcggcg gcgtagggcg cgctgcccga
     3301 accgaacgcc gagacctcgg tgatctcgat gtccgaggtg tcgcccccgg gacccccggg
     3361 atcggccggc gcgacgttgc ccggcgagcc gagcccgatg ccgtccgcac cgacgaaggc
     3421 gccgaagacg ccttcgcccg ccagggtctt gatccccacg ggtgccgcca cggcgccgag
     3481 ccgagcggtg ttgtcgaagg tgacgtcggc ggtcgatccg gcgaaggaca cgccggtgac
     3541 gtgcgtgccg ggggcgaaca ggttgaccgc atcgcccccg gacgacagcc cgaggcccga
     3601 gccgctgtag gagccgatct tgaagccgtc ggggagcccg gcatcgccga accacgcctg
     3661 tccgaaggcg gcagcgatcg cagcggcatc gccgctggtg ctcttctcga agaagatcgc
     3721 cgactcaccc gatgccaggc tgctgacccc ggtgagggcc gcggcggaac cgaaggcgtt
     3781 ggacgagtcg tccaccttca cgccatcgag gtcgagggtg gtcgcaccgg tgttggtcag
     3841 ctcgaaccag tcggcaccgt aggaagcgtc gctgccccac ggcgccacct cggtgacagc
     3901 cagccgggcg agcggcgatg ccgcaggcgc ccacaccagc agctgcgggt gggaggcatc
     3961 gccaccgccc ccttcggacg tcgtgtacat cacgaggtcg cggtcgagcg cgacgccctc
     4021 catcgtctgg gcgggcacgc tcaggggagc gccggcatcg acgatgtcga gctggttggc
     4081 gatcgccccg acctcccccg ccccgacctg acctcccacg aacttggtgt gcgaatcgag
     4141 gctgggcgtc ggttcgcaca ccaagttcgg atgagagggg cgggaacggg cggcgggagg
     4201 ggggaggggc cgggtcgtgg cgcgtcaggc gagggcgtag cgctgttcgg ccagtcggtg
     4261 gaggcgctgc cagaagagcc ggttgaccgc gatgatgtag agcgacatca cggtgacgcc
     4321 ggcgaggatc cggtggaagt cgccggtctg ggtggcgtgg gcgatgaacg cgccgaggcc
     4381 cgtggcggtg agggtggtgc tcccgtaggt gacgatctcg gcgacgatgg cggcgttcca
     4441 cgccccgccc gatgcggtga tgccaccggt gacgtagctg gggaacacgg cagggaggat
     4501 gaggcgcttc cagcggagcc agccgccgat cccgaggtcg tccatggcct cgatcaggtc
     4561 accggggatc gactgggcgc cggcgatggt gttgaacagg atgtaccact gcgcgcccag
     4621 ggccatgagc acgatgccgc cgacgtcgag ggtgagcccg gtgcggacga aggcccacac
     4681 cgcgaacggg aacaagaagt tggccgggaa gctggcgagg acctgcacga tcggctgggc
     4741 gatgcgagac gccctggggt gcaggccgat cgcgacgccg atcgggaccc agacgacggt
     4801 cgagagggcg acgacggcca cgacccgggc gagggtcagc agtccctgcc acagcggtgg
     4861 gacgaacacg ccccagccgt cgaccgcggt cacgtaccgg agcagttcga cggtgccgta
     4921 gccgatgacg ccgacggtga cggcggcgaa cacgaggtcg ccacggcgac gcgtccccgt
     4981 atccgcatcg acggggcgtt cgtggccgaa cacgcgatca ccggcgcggt tgagcggctc
     5041 ggcgaggcgt cgccgcgctc gaccgacggc gcgcggccag tgggagcgcc ggaggaagtc
     5101 gagcaccatc gaacgcggga cctgctcgga ctcgctctgc tcgttcttga accgctccgc
     5161 ccacgccacc agcggccgcc agaacatgaa gttcaccccc agcaccatga gggccatggc
     5221 gacagccccg atggccacct tgcggaggct gccctcggtg atggccgcgc cgacgtacga
     5281 gccgatgccg ggcagggcgt agctgcggtt gaggacgctg atcgactcgg aggcgaccag
     5341 gaagaaccag ccgcccccca tgctcatcat cccgttccag acgaggccga tcgcgccgct
     5401 ggggacgtcg agcttccaga accggcgcca gcgcgacagc ccgaggagcc gggcggcttc
     5461 gtcgaggtcc c
//
LOCUS       DATMFS010000508         4069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_156924, whole genome shotgun sequence.
ACCESSION   DATMFS010000508 DATMFS010000000
VERSION     DATMFS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156924"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..692
                     /locus_tag="VNQ33_09590"
     CDS             228..692
                     /locus_tag="VNQ33_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWJ98400.1"
                     /translation="MTKTIDPATTRYLAPTAGDRIFNRFAGWLTRHGVSISGSRELRI
                     VGRKSGQIRTNVVNLLVLNGERYLVAPRGITEWVRNLRAAGQGELKVGRRVEAFRAEE
                     LADADKPPALREYLERWAWEVGRFFEGIDASSPDEDLLAIAPGFPVFRLLEP"
     gene            804..2192
                     /locus_tag="VNQ33_09595"
     CDS             804..2192
                     /locus_tag="VNQ33_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWJ98401.1"
                     /translation="MHRGPYADGPPPEALEVHAPPLDRFGVTELRRMLLIGFVLFAAV
                     LRSCAGWIVRRQGRSFATAASEGVVEGFDQLGPTFVKLGQLIASSPSLFPTPLADACL
                     KMLDEVRPFDPATVRRLVEEQLGAPPEAVFAAFDDVPLSAASIAQVHACTLPDGREAV
                     IKLQRPDIAHRMNTDLRIQYFFATKLISRLNFAKRANVVGMVEDLHDVTNQELNAALE
                     AHRQTYFRDHIGAFGDNAGITAPEVYWNFCGPHLICMERMRGVPMDEFDSIAALGVDG
                     ELILRRGIKVWVEAALIHGTFHGDVHAGNLWVLEDGRASYLDFGIMGELPELWRDTMR
                     EILYTSTIDQDYRRVVRAYQKVGVIPDDVDPEVAGPAIAMVMEPMLTQGIGAVSLGDQ
                     LKANLELANQYGATAPKELLLVSKQLMYFERYAKTLAPGYVLARDLFLLQNVFPEAVA
                     EAAAERGVTLPE"
     gene            complement(2205..2777)
                     /locus_tag="VNQ33_09600"
     CDS             complement(2205..2777)
                     /locus_tag="VNQ33_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98402.1"
                     /translation="MAPSANCPTCRAALQLTNHDTFDSWICPNGHGLAATLSELYERA
                     QEDEIHALWAQAKAAWVAAEPTTGKACPMCERIMVSIVVGTDADEVDEGQPGDGPNTG
                     EVPIDVCVVDQVIWFDVNELELFPADLPDPQPTPEQEAAVQDIARRFADDVAAVQEAR
                     TIGFTDRLLRRLHLSPAAASAVNPTARADA"
     gene            2882..>4069
                     /locus_tag="VNQ33_09605"
     CDS             2882..>4069
                     /locus_tag="VNQ33_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWJ98403.1"
                     /translation="MTDQRTPDRPWMMRTYSGHSTAKASNELYRTNLAKGQTGLSIAF
                     DLPTQTGYDSDDPMARGEVGKVGVPVSHIGHMETLLDQIPVGEMNTSMTINAVAAWML
                     GLYTANAERQGVKSQQLRGTTQNDIVKEYLSRGTYIYPPLPSRRLIVDMFAFCQEWIP
                     HWNPMNVCSYHLQEAGATPVQEIAYSLATAIDVLDAVKESGQVPDDRMVNVVGSISFF
                     VNAGIRFVEETCKMRAFTQMWDRICLERYGITDAKARRFRYGVQVNSLGLTEAQPENN
                     VQRIVLEALGVTLSKDARARSLQLPAWNEALGLPRPWDQQWALRIQQVLAFETDLLEY
                     DDIFAGSHVVEAKTKDLIDGAQAELDEVLALGGAFEAIEELKGRLVRSHAERMRKIES
                     GELT"
BASE COUNT          640 a         1471 c         1349 g          609 t
ORIGIN      
        1 cccgcagcga caggccgtcg gacccgacct cggcgagctg ccgccgcgcc gcggccttga
       61 tctcggcgat gcgctcgatc cgggccaggg cgcgcggcgt gctcgggtcg atcggcatgc
      121 ttccacgcta gcgcaaattg cgagagcact gctcttgaca tggagccgat ctgagagaac
      181 actgctcact attcagagag cgttgctctc ccgttacagg agccaccatg acgaagacca
      241 tcgaccccgc caccacccgc tacctcgccc ccaccgccgg cgaccgcatc ttcaaccggt
      301 tcgccggttg gctcacccgc cacggcgtga gcatctcggg gtcgcgcgag ctgcggatcg
      361 tgggccgcaa gtccggccag atccgcacca acgtcgtgaa cctgctcgtg ctcaacggcg
      421 agcgctacct cgtcgcccct cgggggatca ccgagtgggt gcgcaacctg cgggccgccg
      481 gccagggcga gctgaaggtc ggacggcgcg tcgaggcgtt ccgcgccgaa gagctggccg
      541 acgccgacaa gcccccggcc ctgcgcgagt acctcgagag gtgggcctgg gaggtcggcc
      601 gcttcttcga gggcatcgac gcctcctctc ccgacgagga cctcctcgcc atcgcccccg
      661 gcttccccgt cttccgcctg ctcgagccct gatcggctcc tgtcgccgtt cgcgccgtct
      721 agggacacgt tctgcgacag aaccgggctg gaccggttca gaccgtcctg ggtggcgccg
      781 tggccagagg gtacggtctc gccgtgcacc gcgggcccta cgcagacgga ccgcctcccg
      841 aggcgctgga ggtccatgcg ccgccgctgg accgcttcgg ggtcaccgag ctgcgccgga
      901 tgctgctgat cggcttcgtg ctcttcgccg ccgtcctgcg gtcgtgcgcc gggtggatcg
      961 tgcgccgcca gggccggtcg ttcgcgaccg ccgcatccga aggggtggtc gaggggttcg
     1021 accagctcgg gccgacgttc gtgaagctcg gccagctcat cgccagctca cccagcctct
     1081 tcccgacacc gctcgccgac gcctgcctga agatgctcga cgaggtccgg ccgttcgacc
     1141 cggccaccgt gcggcgcctc gtggaggagc agctcggcgc cccgcccgaa gcggtcttcg
     1201 ccgccttcga cgacgtgccc ctctcggccg cgtccatcgc ccaggtccac gcctgcacgc
     1261 tgccggacgg gcgggaggcg gtcatcaagc tccagcggcc cgacatcgcc caccgcatga
     1321 acaccgatct gcggatccag tacttcttcg ccacgaagct gatcagccgg ctcaacttcg
     1381 ccaagcgagc caacgtcgtg ggcatggtcg aggacctgca cgacgtcacc aaccaggagc
     1441 tcaacgccgc gctcgaagcc caccgccaga cgtacttccg agaccacatc ggcgccttcg
     1501 gcgacaacgc cggcatcacc gcccccgagg tctactggaa cttctgcggc ccccacctga
     1561 tctgcatgga gcggatgcgc ggcgtcccca tggacgagtt cgactccatc gctgcgctgg
     1621 gcgtcgacgg cgagctgatc ctgcgccgcg gcatcaaggt gtgggtcgag gccgccctca
     1681 tccacggcac gttccacggc gacgtccacg ccggcaacct ctgggtgctc gaagacgggc
     1741 gggccagcta cctggacttc gggatcatgg gcgagctgcc cgagctgtgg cgcgacacga
     1801 tgcgggagat cctgtacacg tcgaccatcg accaggacta ccggcgggtc gtgcgggcgt
     1861 accagaaggt cggggtgatc cccgatgacg tggaccccga ggtcgccggc cccgccatcg
     1921 ccatggtcat ggagccgatg ctcacgcagg ggatcggcgc ggtcagcctg ggcgaccagc
     1981 tcaaggccaa cctcgagctg gccaaccagt acggcgccac ggcgcccaag gagctgctcc
     2041 tcgtgtcgaa gcagctcatg tacttcgagc ggtacgccaa gacgctggcg ccgggctacg
     2101 tgctggcccg cgacctcttc ttgctgcaga acgtgttccc cgaagccgtc gccgaagcgg
     2161 ccgccgagcg cggggtcacc ctgcccgagt gacgaccaga aggatcaggc gtcggcgcga
     2221 gcggtcgggt tgaccgccga tgcggcggcg gggctcaggt ggaggcggcg cagcagccgg
     2281 tcggtgaagc cgatggtccg agcttcctgc acggccgcga cgtcgtcggc gaaacggcgg
     2341 gcgatgtctt ggaccgccgc ctcctgctcg ggggtcggct gggggtccgg caggtccgcg
     2401 gggaacagct cgagctcgtt cacgtcgaac cagatcacct ggtccacgac gcacacgtcg
     2461 atgggcacct caccggtgtt cggaccgtcg cccggctgcc cttcgtcgac ctcgtccgcg
     2521 tcggtgccga cgacgatcga gaccatgatg cgctcgcaca tggggcacgc cttgccggtg
     2581 gtgggctcgg ccgccaccca cgccgccttg gcctgcgccc acagcgcgtg gatctcgtct
     2641 tcttgcgccc gctcgtacag ctcggagagc gtcgcggcga ggccgtgacc gttggggcag
     2701 atccacgagt cgaaggtgtc gtggttggtc agctgcagcg cagcacgaca ggtggggcag
     2761 ttcgctgaag gggccacacg gtcctatcgg ccccttcgag ccgatcttga acgcggcgcc
     2821 gacgggccgg ccgggcagcc tcagcgtgac tttccctgcg tccacggcgg agaatggacc
     2881 catgaccgat cagcgcacgc cggaccgccc ctggatgatg cggacctact cgggtcactc
     2941 caccgccaag gcgtcgaacg agctgtaccg caccaatctg gccaagggcc agaccggcct
     3001 gtcgatcgcc ttcgacctcc ccacccagac gggctacgac agcgacgacc cgatggcccg
     3061 cggcgaggtc ggcaaggtcg gcgtgccggt gagtcacatc ggccacatgg agacgctgct
     3121 cgatcagatc cccgtcggcg agatgaacac gtcgatgacc atcaacgccg tcgccgcgtg
     3181 gatgctcggg ctctacaccg ccaacgccga gcgccagggc gtgaagtccc agcagctccg
     3241 ggggaccacc cagaacgaca tcgtcaagga gtacctgagc cgcgggacgt acatctaccc
     3301 gccgctgccc tcccgccggc tgatcgtcga catgttcgcg ttctgccagg agtggatccc
     3361 gcactggaac ccgatgaacg tctgcagcta ccacctgcag gaggcggggg cgacgccggt
     3421 ccaggagatc gcctactccc tggccaccgc catcgacgtg ctcgatgccg tgaaggagtc
     3481 gggccaggtg cccgacgacc gcatggtgaa cgtggtcggg tccatctcgt tcttcgtcaa
     3541 cgccggcatc cgcttcgtgg aggagacctg caagatgcgg gccttcaccc agatgtggga
     3601 ccggatctgc ctggagcgct acggcatcac cgacgccaag gcccgccggt tccgctacgg
     3661 cgtgcaggtc aacagcctcg ggctcaccga agcccagccc gagaacaacg tgcagcgcat
     3721 cgtgctggag gccctcggcg tgaccctgtc gaaggatgcc cgggcccgct cgctccagct
     3781 cccggcgtgg aacgaggccc tcggcctgcc ccgcccctgg gaccagcagt gggcgctgcg
     3841 catccagcag gtgctggcgt tcgagaccga cctgctcgag tacgacgaca tcttcgccgg
     3901 ctcccacgtg gtcgaggcca agaccaagga cctcatcgac ggtgctcagg ccgagctcga
     3961 cgaggtcctc gccctcggcg gcgccttcga ggccatcgag gagctgaagg gccggctcgt
     4021 gcgcagccac gccgagcgga tgcgcaagat cgagtccggc gagctcacc
//
LOCUS       DATMFS010000509         2276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_112225, whole genome shotgun sequence.
ACCESSION   DATMFS010000509 DATMFS010000000
VERSION     DATMFS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112225"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..603
                     /locus_tag="VNQ33_09610"
     CDS             145..603
                     /locus_tag="VNQ33_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWJ98404.1"
                     /translation="MASASSTSSVSTTKRRQIVLATVLCAMALLTGCLRSTQSQVLNE
                     LNADRSANKLRTLPTQADAQAKAQAWAEKLARENTLYHSTLSQGIKVKWCSLGENVGY
                     GPSVPAIEDAYMNSSGHRANILSSKWNGVGVGYATNGQRVFTVQVFIKTC"
     gene            complement(937..1731)
                     /locus_tag="VNQ33_09615"
     CDS             complement(937..1731)
                     /locus_tag="VNQ33_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98405.1"
                     /translation="MSGRSVTGPTGNSHAEADVDVPEPSPVPAPGPLRTKLTRSDIGV
                     LLDELDEAMTEAEITGMIELVGGAALALAYYDRLGTTDVDGAIYPSPEIIALADEVGA
                     RHGLKAGWLNNAAQGFIPPGDTGDEPQTVRQGTSLTVTAAGARTLLAMKLRAARPIKD
                     ADDIAFLLRECGVTSLEGAKAVLEEMYNGEHELTERGERMVLGALGARSITQADGQVL
                     QLHAVTSLEPVVCGKWAFRLDRRCDLPPDHTGDCAGGSAEGIAGSV"
     gene            complement(1773..>2276)
                     /locus_tag="VNQ33_09620"
     CDS             complement(1773..>2276)
                     /locus_tag="VNQ33_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98406.1"
                     /translation="AASSKHLQPAISRIEREGGASFHTLEHLLGSLGLRVIAVPLTRP
                     TVADHAIELRQRVAAKKPTIRALAEATNNLVEADPRELPLLVATPPAPTTDARVDAYL
                     AALVDHVCGDTAPHWTDEASRTVTEPWILNPFPADAALDQLIRANTPPAFARHGVYID
                     ASDLASI"
BASE COUNT          347 a          776 c          782 g          371 t
ORIGIN      
        1 cggcaccgcc gcgtgggccg ttcgtaccat ttcttgacgg acccatcacc gaacccgcag
       61 gccaggccac tgagggtggg aagttcgcca cccagggcgg gattcctcgc tcaagacgga
      121 tcagacatgg gccgagaacg aggtatggct tctgcgtcct ccacctcctc ggtctccacc
      181 accaagcgcc gccagatcgt cttggcgacg gtcctgtgcg cgatggctct gctcaccggc
      241 tgcctccgca gcacccagtc gcaggtgctc aacgagctca acgccgaccg ttcggccaac
      301 aagctccgca ccctccccac ccaggccgac gcccaggcca aggcgcaggc gtgggccgag
      361 aagctggctc gggagaacac gctctaccac tcgaccctct cccagggcat caaggtcaag
      421 tggtgctcgc tcggcgagaa cgtgggctac ggcccgagcg tgcccgccat cgaggacgcc
      481 tacatgaact cctcgggtca ccgcgccaac atcttgagct ccaagtggaa cggcgtcggc
      541 gtcggctacg ccaccaacgg ccagcgagtc ttcaccgtcc aggtcttcat caagacctgc
      601 tgagaggccc ctgggccgtt cgcggttctc tgacctgccg tttcgcgcaa ctcgccgcct
      661 gtgacggcct tcgccccgat cggcgctcag ggggaccgtg atcgagcccc ggaaggtcac
      721 tcaaccggct gatgtcccca ccgggcgacg agagggccaa cacggtgccg gccccgggcg
      781 cggcctgcga aaccccgagg cgttcttccg ctcggggtcc tccgaccgct gccgcgagac
      841 gctctccgag agcgatcgcc agacctgcga ggccctcgtc cacgagctag cccccgatga
      901 ggacgcccgc tggctgtggc gttgacgcgc aggccatcaa acgctgcctg caatgccctc
      961 tgccgagcct ccggcgcaat cgccggtgtg gtcgggcggg agatcacagc ggcggtccaa
     1021 cctgaaggcc cacttcccac acaccacggg ttcgagcgag gtcaccgcat ggagctggag
     1081 cacctggccg tccgcctggg tgatcgagcg ggcgccaagc gcgccgagca ccatccgttc
     1141 gccacgttcg gtcagctcgt gctcgccgtt gtacatctct tcgagcacgg ccttggcacc
     1201 ctcaaggctg gtgacgccgc actcccggag gaggaaggcg atgtcatcgg cgtccttgat
     1261 cggcctggca gctcggagct tcatggcgag cagcgtcctc gccccggcag cggtcacggt
     1321 gagcgatgtg ccttgacgga cggtctgggg ctcgtcgccg gtatcccccg gcgggatgaa
     1381 gccctgagca gcgttgttca gccacccggc cttgagcccg tggcgagcac cgacctcatc
     1441 agccagggcg atgatctccg gcgatggata gatggctccg tcgacgtcgg tcgtgccgag
     1501 ccggtcgtag taggcgagcg ccagcgccgc tccgcccacg agctcgatca tgccggtgat
     1561 ctctgcttcg gtcatggctt catcgagctc gtcgagcagc accccgatgt cggacctggt
     1621 cagcttggtc cggagcggcc caggcgcggg gaccggtgac ggttcgggca cgtcgacgtc
     1681 agcctcagcg tgcgagttcc cggtgggtcc ggtgacggaa cgcccgctca cttggtgatc
     1741 gttggtcccc cgtccgccgc cggtcttgcc catcagatgc tcgccaggtc agacgcatcg
     1801 atgtagacac cgtggcgggc gaacgccggc ggggtgttgg ctcggatcag ctggtcaagt
     1861 gcagcgtcgg cggggaacgg gttgaggatc cacggctcgg tgaccgtgcg gctcgcctcg
     1921 tcggtccagt ggggcgcggt gtcaccgcag acgtggtcga cgagcgctgc gaggtaggcg
     1981 tcgacgcggg catcggtggt cggcgccggc ggggtggcca ccagcagagg cagctctcgg
     2041 gggtcagctt ctacgaggtt gttggtggct tcggcgaggg cccggatcgt cggcttcttg
     2101 gcagcgacgc gctggcgcaa ctcgatggcg tggtcggcaa cggtaggtcg ggtgaggggc
     2161 acggcgatga ctcggagacc gaggctgccg agcaagtgct cgagcgtgtg gaaggaggcc
     2221 ccgccttcgc gctcgatccg gctgatggcc ggctggaggt gcttggagga cgcggc
//
LOCUS       DATMFS010000510         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_271176, whole genome shotgun sequence.
ACCESSION   DATMFS010000510 DATMFS010000000
VERSION     DATMFS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271176"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VNQ33_09625"
     CDS             <1..229
                     /locus_tag="VNQ33_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWJ98407.1"
                     /translation="FELAQVMCLDALTREESCGGHFRAEHQTDEGEAERDDEHFAHVA
                     AWEWTGDAGNPIRNTEPLTFDNVHLAVRSYK"
     gene            232..1002
                     /locus_tag="VNQ33_09630"
     CDS             232..1002
                     /locus_tag="VNQ33_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWJ98408.1"
                     /translation="MTDLNLTLKVWRQAGPDAPGSFDTIEAQGINDEMSFLEMLDIVN
                     ERLIDDGKEAIVFDHDCREGICGTCSLMINGQAHGPQKGTATCQLHMRKFHDGQEIVI
                     EPWRATAFPIVKDLMVDRSAFDRIVEAGGFITAETGGASDANLTPIPKPVADAAMDAA
                     ACIGCGACVAACPNSAANLFTSAKASHLNLLPQGQAERYSRVEAMVDTMEDFFGSCTN
                     HGECQEACPKEISIDFIALMNKDYLASKFKNRRMLSRK"
     gene            1100..1816
                     /locus_tag="VNQ33_09635"
     CDS             1100..1816
                     /locus_tag="VNQ33_09635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ98409.1"
                     /translation="MELILVRHGQPAWTTPDGKGRNDPGLTELGHSQAERAAARLADT
                     EDLPGRGDVDRLFASPAVRAQETAGPIAAALDLPIETHEWLWELRNPDAWEGAPIEEI
                     EQAFVTLGSQSREVWWQGHEGGESLRAFHQRVTTGLRALLADIGITPAAEDGLWDVAG
                     PAADGTIDRVVAVAHGGTNSTIIAHLLGAAPEPWEWDRFSMGHASVALLVLTPLAGHH
                     IWSLRSLGDAAHLAVVDRTV"
     gene            1900..>2878
                     /locus_tag="VNQ33_09640"
     CDS             1900..>2878
                     /locus_tag="VNQ33_09640"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HWJ98410.1"
                     /translation="MPRSVRRRAWSAGLVMGLLVALAACSSGRSDGAEDPSTTKGTQK
                     TTTTMLQPEGPAIDRYAGYESASYDDPSHWVCRPDQDDICDHGLDATVVEADGTLTVE
                     PFTPSADPPVDCFYVYPTISRDKGAYSDWEASDEEEGYVTLNQAARLQSECRLFAPVY
                     RQGTLGGLAARINGETPDEQGDPYADVLDAFRTYMAEDNHGRGVVLIGHSQGTGMLHQ
                     LISEEIDPNPDVRKLLVGAYLAGGSVSVPEGKAVGGDFDSVPLCTKQGETGCVVNWVT
                     FRSTSPPPSDSFFGRSGRDGLAAGCVNPAAVGGGSGDLDAYLPANSEASI"
BASE COUNT          455 a         1073 c          968 g          382 t
ORIGIN      
        1 cttcgagctc gcccaggtca tgtgcctcga cgccctcacc cgtgaggagt cctgcggcgg
       61 ccacttccga gccgagcacc agaccgacga gggcgaggcc gagcgcgacg acgagcactt
      121 cgcccacgtc gccgcctggg agtggaccgg tgacgccggc aaccccatcc gcaacaccga
      181 gcccctcacc ttcgacaacg tccacctcgc cgtccggagc tacaagtagc gatgaccgat
      241 ctcaacctca cgctcaaggt ctggcgccag gccgggccgg acgcccccgg gtccttcgac
      301 acgatcgaag cccagggcat caacgacgag atgtcgttcc tcgagatgct cgacatcgtc
      361 aacgaacggc tcatcgacga cggcaaggaa gcgatcgtct tcgaccacga ctgccgcgag
      421 ggcatctgcg gcacgtgctc gctgatgatc aacggccagg cccacggacc gcagaagggc
      481 acggccacct gccagctcca catgcggaag ttccacgacg gccaggagat cgtgatcgag
      541 ccctggcggg cgacggcgtt ccccatcgtc aaggacctga tggtcgaccg ctcggcgttc
      601 gaccgcatcg tcgaggccgg cggcttcatc accgccgaga ccggcggcgc gtccgacgcc
      661 aacctcaccc ccatcccgaa gccggtggcc gacgcggcca tggacgccgc cgcctgcatc
      721 ggctgcggcg cgtgcgtggc ggcgtgcccc aactcggccg ccaacctgtt cacgtcggcg
      781 aaggccagcc acctcaacct gctgccgcag ggccaggccg agcggtacag ccgggtggag
      841 gccatggtcg acaccatgga ggacttcttc gggtcctgca ccaaccacgg cgagtgccaa
      901 gaggcgtgcc ccaaggagat ctcgatcgac ttcatcgccc tcatgaacaa ggactacctg
      961 gcctccaagt tcaagaaccg ccggatgctc agccgcaagt aggcgcatcc cgctccgctc
     1021 cgaggcccgc accgatccgg tgcgggcctc ggtcgtttcg gagccgtcgg tgtcggaggg
     1081 ggtcggtagg gtcggcggca tggagctgat cctcgtccga cacggccaac cggcgtggac
     1141 cacgcccgac gggaagggcc gcaacgaccc cgggctcacc gagctgggcc acagccaggc
     1201 cgagcgggcg gcggcgcggc tggccgatac ggaggacctg ccggggcgcg gcgacgtcga
     1261 ccggctgttc gcctcgcccg cggtccgggc ccaggagacg gcggggccca tcgccgccgc
     1321 gctggacctg ccgatcgaga cccacgagtg gctgtgggag ctgcgcaacc ccgatgcatg
     1381 ggagggcgca cccatcgagg agatcgagca ggcgttcgtc acgctcggct cccagtcccg
     1441 cgaggtgtgg tggcagggcc acgaaggcgg ggagtcgctc cgagcgttcc accagcgggt
     1501 gaccaccggc ctgcgcgccc tcctggccga catcggcatc acgccagccg cggaggatgg
     1561 gctgtgggac gtggccgggc ccgccgccga cgggacgatc gaccgggtgg tggccgtggc
     1621 ccacggcggc accaactcca cgatcatcgc ccacctcctc ggggccgccc ccgagccctg
     1681 ggagtgggac cgcttctcca tgggacacgc gtcggtggcc ctcctggtcc tcacgccgct
     1741 ggccggccac cacatctggt cgctgcgctc gctcggcgat gccgcccacc tcgcggtcgt
     1801 ggaccggacg gtgtgaccca ccgggttcct gccgcgcctc ggctacccat ggcctgcgtg
     1861 aggacgacac ggcccgcggg tcggcagtac ggtcaccgga tgccccgctc tgtccgtcga
     1921 cgcgcctggt cagcgggcct ggtgatgggg ctcctcgtcg ccctcgccgc ctgcagctcg
     1981 ggccgcagcg acggggccga ggatccgtcc accaccaagg gcacccagaa gacgaccacc
     2041 accatgctcc agcccgaggg accggccatc gaccgctacg ccggctacga gagcgccagc
     2101 tacgacgacc cgtcgcactg ggtctgccgt cccgaccagg acgacatctg cgaccacggc
     2161 ctcgatgcca cggtggtcga ggcagacggc accctcacgg tcgagccgtt cacgccgtcg
     2221 gctgacccgc ccgtcgactg cttctacgtg tacccgacga tctcgcgcga caagggggcc
     2281 tacagcgact gggaggcgtc cgacgaggag gaggggtacg tcaccttgaa ccaggcggcg
     2341 cgcctccagt ccgagtgccg gctgttcgca cccgtctacc ggcagggcac cttgggtggt
     2401 ctcgccgccc ggatcaacgg cgagacgcca gacgagcagg gcgacccgta cgccgacgtc
     2461 ctcgacgcct tccggaccta catggccgag gacaaccacg gccgcggcgt cgtgctcatc
     2521 ggccactcgc agggcaccgg gatgctccac cagctcatca gcgaggagat cgacccgaac
     2581 cccgacgtgc gcaagctcct ggtgggcgcc tacctcgccg gcggttcggt cagcgtgccc
     2641 gagggcaagg cggtcggcgg tgacttcgac tcggtgccgc tctgcacgaa gcagggcgag
     2701 accggctgcg tcgtcaactg ggtcacgttc cggtcgacct cgccgccgcc gagcgactcg
     2761 ttcttcggcc ggtccggccg tgacggcctg gccgccggat gcgtgaaccc cgcggcggtc
     2821 ggcggcggct ccggcgatct cgacgcctac ctgcccgcca actccgaggc gtcgatcc
//
LOCUS       DATMFS010000511         3436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_87657, whole genome shotgun sequence.
ACCESSION   DATMFS010000511 DATMFS010000000
VERSION     DATMFS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87657"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VNQ33_09645"
     CDS             complement(<1..741)
                     /locus_tag="VNQ33_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWJ98411.1"
                     /translation="MASASSTATEAPAPPADGAIEGDGGRSRREAPILLSVVALAGLL
                     LLTVVVATGVGAVGIARSNVLSTVVHHLGLGSGPDAAGPVDGIVWNLRLPRILMAALC
                     GMGLAGVGVVMQGVTRNPLADPYLLGLSSGASLGAVTVVALGLGAVGSPALTLGAFIG
                     ATVAFALVLVLASGDGRLPPVRTILAGVAIGEAMAAATSFVTISSTNAQATRSLLVWL
                     LGGFAGTSWADLPLVAALVLAFAIASRGD"
     gene            complement(713..1738)
                     /locus_tag="VNQ33_09650"
     CDS             complement(713..1738)
                     /locus_tag="VNQ33_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98412.1"
                     /translation="MPSSSRIARACGATLLSCLLLSTTACGSSTSDGAPSDDTEATTT
                     EAGRADSTFPMTIENCGETVTLDAPPTKAITMNQGATETMLALGLEDQMIGTAYLDDA
                     VLPEWQDVYDSVDVLSDEYPSQEEVLATGTDFVYGSYASAFDDEAAGSRGSLAKEDIG
                     TYLSPNDCQASDGTPVTFDDVFAEITEIGAVFGVPDRAAALVADSKDRLAEATADLPE
                     GQTVLWYDSGDDAPFVGACCGGPNLVMSAVGATNVFDDLQGGWEDASWEAVVAADPDA
                     IVLVDASWDTVAAKKKVLAAAPMNAMTAVEDEQFIEIPFSSSSPGVRNVSAVEQLADG
                     LRKLDGN"
     regulatory      complement(1780..1859)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1944..2360)
                     /locus_tag="VNQ33_09655"
     CDS             complement(1944..2360)
                     /locus_tag="VNQ33_09655"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWJ98413.1"
                     /translation="MTDTGRPTALFLCVHNAGRSQMALGWFEHLAAGRATAWSGGSEP
                     ADQINPVAVAAMAEVGIDIAAASPKRWTDEAVRAADVVITMGCGDACPFYPGTRYEDW
                     ELDDPAGRGLDAVRPIRDDIERRVRALLAELGVPAS"
     gene            complement(2357..3079)
                     /locus_tag="VNQ33_09660"
     CDS             complement(2357..3079)
                     /locus_tag="VNQ33_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWJ98414.1"
                     /translation="MSAPTGALGRRVLAEGLGTGLLVAIVVGAGIAAQRLSPGDTGLQ
                     LLESALATGAGLVALILAFGSVSGAHFNPVVSLADWLLGGLTGGATAAYLAAQVTGAC
                     AGCIVANLMFDLAPVTLSDTTRSSSGTWLGEVVATFGLLVVIFGLARSGRGALAPFAV
                     AAYITAAYWFTSSTSFANPAVTIGRTLTDTFAGIAWSSVPAFIAFQLVGGALALVVSR
                     ALHPDLDPDDVVVPHAATGGTP"
     gene            complement(3081..>3436)
                     /locus_tag="VNQ33_09665"
     CDS             complement(3081..>3436)
                     /locus_tag="VNQ33_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98415.1"
                     /translation="PPLKLVLIEAEEGGTLNHLGVEVDSAEEVVAAEGRLSADGLATT
                     GVDDTTCCYALKTETWVNDPDGAPWEWYVKTGDAEQQGSTVRSGGGTGACCSTAAISQ
                     SVSLGAAPGAASGCC"
BASE COUNT          492 a         1139 c         1373 g          432 t
ORIGIN      
        1 gtcgccacgg gatgcgatgg cgaaggcgag gaccagcgcg gccacgaggg ggaggtcggc
       61 ccacgacgtg ccggcgaagc cgccgagcag ccacacgagc aggctccggg tggcttgggc
      121 gttcgtcgag ctgatggtga cgaacgaggt ggcggcggcc atcgcctcgc cgatggcgac
      181 gcccgcgagg atggtccgca ccggcgggag ccggccgtcg ccgctcgcca gcacgaggac
      241 gagggcgaac gccacggtcg cgccgatgaa ggcgccgagg gtgagggcgg gcgaacccac
      301 cgcgccgagg ccgagggcca ccacggtcac ggcgccgagc gaagctcccg acgacaggcc
      361 gagcaggtac gggtccgcca gcgggttgcg ggtcaccccc tgcatcacga cgccgacgcc
      421 ggccaggccc atgccgcaca gcgcggccat gaggatgcgg ggcaggcgga ggttccagac
      481 gatgccgtcg accggaccgg cggcgtcggg cccggagccg agtccgaggt gatgcaccac
      541 cgtgctgagc acgttcgagc gggcgatccc gacggcgccc acgccggtgg ccacgacgac
      601 cgtgaggagg agcagtcccg ccagggcgac gacggagagg aggatggggg cctcccgccg
      661 agagcggccc ccatccccct cgatcgcgcc gtccgctggg ggagcgggcg cctcagttgc
      721 cgtcgagctt gcggaggcca tcggcgagct gctcgacggc gctcacgttg cggacgccgg
      781 gggagctcga cgagaacggg atctcgatga actgctcgtc ctcgacggct gtcatggcgt
      841 tcatcggcgc cgcggcgagg accttcttct tcgccgcgac cgtgtcccag gaggcgtcga
      901 ccaagacgat ggcgtcgggg tcggccgcga ccaccgcctc ccaggaggcg tcctcccagc
      961 cgccctgcag gtcgtcgaac acgttcgtgg cgccgaccgc gctcatgacg aggttcgggc
     1021 cgccgcagca ggcgccgacg aagggggcgt cgtcgccgga gtcgtaccag aggacggtct
     1081 ggccctcggg caggtccgcg gtggcctcgg ccaggcggtc cttgctgtcc gccaccagcg
     1141 cggcggcccg gtcgggcacg ccgaagacgg cgccgatctc ggtgatctcg gcgaacacgt
     1201 cgtcgaacgt gaccggcgtc ccgtcggagg cctggcagtc gttgggggag aggtaggtgc
     1261 cgatgtcctc cttggccagc gagccgcggg agccggcggc ctcgtcgtcg aaggcgctgg
     1321 cgtaggagcc gtagacgaag tcggtgcccg tcgccagcac ctcctcctgg gaggggtact
     1381 cgtcggagag gacgtcgacg gagtcgtaga cgtcctgcca ctcgggcagc acggcatcgt
     1441 cgaggtaggc ggtgccgatc atctggtcct cgagcccgag ggcgagcatc gtctcggtcg
     1501 cgccctggtt catggtgatg gccttggtgg gcggtgcgtc gagcgtgacg gtctcgccgc
     1561 agttctcgat cgtcatcggg aaggtcgagt cggcgcggcc ggcctcggtc gtcgtggcct
     1621 cggtgtcgtc cgacggcgcg ccgtcgctgg tcgacgagcc gcaggccgtg gtgctgagca
     1681 gcaggcagga gaggagggtc gcaccgcagg cgcgggcgat ccgagatgag ctgggcatgg
     1741 tgacgtgttc ctcgcgtcgt cgggtcggtg ctgccgtgcg gtgctctggc tcccggctcg
     1801 caggctgcga ggaccggtga cagttgcggg cacagccccg gattcgcacc ggtgttcccc
     1861 gctcggcgtg ggcaacgtac cgacgccgcc gtcagccggg caacgccaga cccggtccgc
     1921 ctttcagcgc taaggacccg gtgctacgag gcgggaacac cgagctcggc gaggagggcg
     1981 cgcacgcgcc gctcgatgtc gtctcggatc gggcggacgg catcgaggcc ccggccggcg
     2041 ggatcgtcga gctcccagtc ctcgtaccgc gtgcccgggt agaaggggca ggcgtcgccg
     2101 cagcccatgg tgatcacgac gtcggcggcg cggacggcct cgtcggtcca ccgcttgggc
     2161 gatgccgcgg cgatgtcgat gccgacctcg gccatggccg cgaccgcgac ggggttgatc
     2221 tggtcggcgg gctcggaacc gccggaccag gcggtggctc gtccggcggc gaggtgctcg
     2281 aaccacccga gggccatctg cgagcgcccg gcgttgtgca cgcagaggaa cagggcggtg
     2341 ggccggccgg tgtcggtcac ggggttcctc cggtggcggc gtgggggacc acgacgtcgt
     2401 cggggtcgag gtcggggtgg agggcgcggg agacgacgag ggcgagggca ccgccgacga
     2461 gctggaaggc gatgaacgcc gggaccgacg accaggcgat gcccgcgaac gtgtcggtga
     2521 gggtccggcc gatggtgacg gcagggttgg cgaagctggt ggacgaggtg aaccagtagg
     2581 cggcggtgat gtaggcggcg acggcgaatg gggcgagcgc cccgcggccg gagcgggcga
     2641 ggccgaagat gacgacgagg aggccgaagg tggcgacgac ctcaccgagc caggtgcccg
     2701 acgacgaccg cgtcgtgtcc gacagggtga caggagcgag gtcgaacatc aggttcgcca
     2761 cgatgcagcc ggcacacgcc ccggtgacct gggcggcgag gtaggccgcg gtggccccac
     2821 cggtcagccc gcccaggagc cagtcggcga gcgacacgac cgggttgaag tgcgcccccg
     2881 acaccgaccc gaaggccagg atcagggcga cgaggccggc gccggtggcg agggcgctct
     2941 cgaggagctg aagtccggtg tcgccggggg agaggcgctg ggcggcgatg ccggcgccga
     3001 cgacgatggc gacgaggagc cccgtcccga gcccttcggc caggacccgg cggcccaggg
     3061 ctcccgtggg tgcggacacc tcagcagcag ccggaggcgg caccgggggc ggcgcccagc
     3121 gagaccgact ggctgatggc ggcggtggag cagcaggcgc cggtgccgcc cccgctgcgg
     3181 acggtgctgc cctgctgttc ggcgtcgccg gtcttcacgt accactccca cggcgcaccg
     3241 tcggggtcgt tcacccacgt ctcggtcttg agggcgtagc agcaggtggt gtcgtcgacg
     3301 ccggtggtgg cgaggccgtc ggccgacagc cggccctcgg cggcgacgac ctcctcggcc
     3361 gagtcgacct cgacgcccag gtggttgagc gtcccgccct cctcggcctc gatcaggacc
     3421 agcttcaggg gcggct
//
LOCUS       DATMFS010000512         4111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_320946, whole genome shotgun sequence.
ACCESSION   DATMFS010000512 DATMFS010000000
VERSION     DATMFS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320946"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VNQ33_09670"
     CDS             complement(<1..295)
                     /locus_tag="VNQ33_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ98416.1"
                     /translation="MARQILVVEDEALIADAIAARLRSEGHEVRVASDGPSGVAYAER
                     FEPDLVILDLMLPGLDGLEVCRQIQRERHVPVLMLTARDDETDLVIGLAVGADD"
     gene            409..2205
                     /locus_tag="VNQ33_09675"
     CDS             409..2205
                     /locus_tag="VNQ33_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWJ98417.1"
                     /translation="MSDYAPPLRDITFLLDHVTPIDELTKLEAYQEIDRDSVSGVLEE
                     FGRLMSDVWAPTNAIGDEQGSHVEGDEVVTPDGHKEAYKAYADAGWGAVPFAEEYGGG
                     GFPWLVGIVQQELLNSANMALAMAPLLTQGAIDAILHHGSEEQKEKYLLKMVSAEWTG
                     SMNLTEPDAGSDVGALRTKAVKQDDGTYRITGQKIFITFGEHPFTDNIVHLVLARTPD
                     APTGTKGISLFIVPKFLVNDDGSLGERNDVHIVSVEHKMGIKASPTCILAYGDRSDGA
                     VGYLVGEENAGMRYMFTMMNNARLGVGVEGLGLAERSYQHALAYAKERKQGFAPGAAR
                     GEKSFIVEHPDVRRQLLTMKAYTESMRVLAYKVAEQIDVLRYGADEPTRTAAQEMVDL
                     LIPLTKSYCTDVAETVTSIGIQVHGGMGFIEETGVAQYYRDTKITQIYEGTNGIQAMD
                     LVGRKLPMRAGGVFNDQLARMKATVEELSGAGDDLAVIHRELSAALEALEDATGWILN
                     TGLADPVEALSAATPYQRLFATTVAGWLMAQQGLAAKRLVDAGSADADVAEVKLVTAR
                     FFAEHLLPQVHGLVAPVKAGKADLFALTADQL"
     gene            2353..3345
                     /locus_tag="VNQ33_09680"
     CDS             2353..3345
                     /locus_tag="VNQ33_09680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWJ98418.1"
                     /translation="MTTGLGRARALGALQSIGASSAGLVRADSVTNEVWLTHDLVVRL
                     NRDASMRLHREAVLSQVLPDEVGYPQVIQHGGEVGSDWLVAQRLPGVPLSRAWPTLTA
                     IARRDAVHQIADRLKAVHQTPCPRLDGLHDVPQLLDPAPSGSQAVQRLLNAIARASQL
                     SNIDSTLMADVTALVSDLAPALDPFDATTIVHGDLTFENILWDGAEVTALLDFEYARP
                     GPPDLDLDVLLRFCALPHLHVAPDYEHLTKAEDYAEVPWWLAEEYPELFSHPRQLDRV
                     RLYSIAWDIRELLAFPPSEPLNRLHKHHPYQRLSQNLRGTHYIDRFNGKASVHY"
     gene            3388..>4111
                     /locus_tag="VNQ33_09685"
     CDS             3388..>4111
                     /locus_tag="VNQ33_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HWJ98419.1"
                     /translation="MGDEPGTPGEGVAARIRAASSTLSPAQRRVADAVLADPQLVAFG
                     TVADLAEEAGASGASVVRLATRLGYHGFVGMQEAAQGELSDRLRPAASRIRDLPPGDV
                     VARALTTSMQAVEATLAGLDRSVFGHAVDLLADPERSVAVIAGTAGAGIAGHAGDELR
                     MVRSGVEVVSGTPVAVGQSIARLGRGDVVLALDLRRYDHWLLDAVDHAVREGAALVAL
                     TDGPASPLARRARVLLVVEGEGI"
BASE COUNT          617 a         1548 c         1385 g          561 t
ORIGIN      
        1 agtcgtcggc ccccaccgca aggccgatga ccaggtcggt ctcgtcgtcg cgggcggtga
       61 gcatcaggac ggggacgtgg cgctcccgct ggatctggcg gcagacctcg aggccgtcga
      121 gccccgggag catgaggtcg aggatcacca ggtcgggctc gaaccgctcg gcgtaggcca
      181 cgccggacgg gccgtcggac gccacccgca cctcgtggcc ctcgctgcgc agccgagcgg
      241 cgatcgcatc cgcgatgagg gcttcgtctt cgaccacgag gatctggcgc gccatcgcct
      301 gaccctaacg aaacggctgc gtggccaggc cggcacctca gccgcgagcg tttctgacgg
      361 gccgtcaggg gcttgggcgg cacaggccgc ggtccgtagc gttgggccat gtctgactac
      421 gcgccgccgc ttcgcgacat cacgttcctg ctcgaccacg tcaccccgat cgacgagctg
      481 accaagctcg aggcctacca ggagatcgac cgcgactccg tctccggcgt gctggaggag
      541 ttcgggcgcc tgatgtccga cgtgtgggcc cccaccaacg ccatcggcga cgagcagggc
      601 agccacgtcg agggcgacga ggtcgtcacc cccgacggcc acaaggaggc ctacaaggcc
      661 tacgccgacg ccggctgggg tgccgtgcca ttcgccgagg agtatggcgg cggcggcttc
      721 ccctggctgg tcggcatcgt ccagcaggag ctgctcaaca gcgccaacat ggccctcgcc
      781 atggccccgc tgctcacgca gggcgccatc gacgccatcc tccaccacgg gtcggaggag
      841 cagaaggaga agtacctcct gaagatggtg tcggccgagt ggaccggctc gatgaacctc
      901 accgagcccg acgccggctc cgacgtcggt gcgctgcgca ccaaggccgt gaagcaggac
      961 gacggcacct accggatcac cggccagaag atcttcatca ccttcggcga gcacccgttc
     1021 acggacaaca tcgtgcacct cgtgctggct cgcacccccg acgctcccac cggcaccaag
     1081 ggcatctcgc tgttcatcgt ccccaagttc ctggtgaacg acgacggcag cctgggcgag
     1141 cgcaacgacg tccacatcgt gtcggtcgag cacaagatgg gcatcaaggc cagccccacc
     1201 tgcatcctcg cctacggcga ccgctccgac ggcgccgtcg gctacctcgt cggcgaggag
     1261 aacgccggca tgcggtacat gttcacgatg atgaacaacg cccgcctcgg cgtcggcgtc
     1321 gagggcctgg gcctggcgga gcgcagctac cagcacgccc tcgcctacgc caaggagcgc
     1381 aagcagggct tcgctcccgg cgccgcccgc ggggagaagt cgttcatcgt cgagcacccc
     1441 gacgtgcgcc gtcagctgct cacgatgaag gcgtacaccg agtcgatgcg ggtcctcgcc
     1501 tacaaggtcg ccgagcagat cgacgtcctc cgctacggcg ccgacgagcc gacccgcacg
     1561 gccgcccagg agatggtcga cctgctgatc ccgctcacca agagctactg caccgacgtg
     1621 gccgagaccg tcacgtcgat cggcatccag gtgcacggcg gcatgggctt catcgaggag
     1681 accggcgtcg cccagtacta ccgcgacacc aagatcacgc agatctacga gggcaccaac
     1741 ggcatccagg ccatggacct cgtgggccgc aagctcccga tgcgcgccgg cggcgtgttc
     1801 aacgaccagc tcgcccgcat gaaggccacc gtcgaggagc tctcgggcgc cggtgacgac
     1861 ctcgccgtca tccaccgcga gctgtccgcc gcgctcgagg ccctcgagga cgccaccgga
     1921 tggatcctca acacgggtct ggccgatccg gtcgaggcgc tgtcggcggc cacgccgtac
     1981 cagcgcctgt tcgccaccac cgtcgccggc tggctcatgg cccagcaggg actggccgcc
     2041 aagcgcctcg tcgatgccgg ctcggccgac gccgacgtgg ccgaggtcaa actggtcacc
     2101 gcccgcttct tcgccgagca cctgctcccg caggtccacg gcctggtggc accggtcaag
     2161 gccggcaagg ccgatctgtt cgccctcacg gccgaccagc tctgacgcct gctcgccccg
     2221 gtcccggggc cgcggcgtcg ggccctcgcc gggtggctgg gcgctaggtc gtttggctca
     2281 cgaagccgtc aggtcggccg atgggacctc ctggtggacc gcacgcacac gacctcctcg
     2341 atcggttcgc cgatgacgac cggcctcggc cgggcacgcg ccctcggcgc gctccagagc
     2401 atcggagcgt cgtcggcggg gctggtgcgc gccgacagcg tcaccaacga ggtctggctc
     2461 acccacgacc tggtcgtgcg gctgaaccgc gatgccagca tgcgcctcca ccgggaggcc
     2521 gtgctgtcgc aggtgctgcc cgatgaggtc gggtaccccc aggtcatcca gcacggcggc
     2581 gaggtgggca gcgactggct ggtggcccag cgcctccccg gcgtgccgct cagccgggcc
     2641 tggcccaccc tcaccgcgat cgcccggcgc gacgccgtcc accagatcgc cgaccggctg
     2701 aaggcggtcc accagacgcc gtgccctcgg ctcgacgggc tccacgacgt gccccagctg
     2761 ctggacccgg cgccgtcggg gagccaggcg gtgcagcgct tgctcaacgc catcgcccga
     2821 gccagccagc tgtccaacat cgactcgacg ctcatggccg atgtgaccgc cctggtgtcg
     2881 gacctggcgc ctgcgctgga cccgttcgac gccacgacca tcgtccacgg cgatctcacg
     2941 ttcgagaaca tcctgtggga cggcgccgag gtcaccgccc tgctcgactt cgagtacgcc
     3001 cggcccggcc cgccggacct cgacctcgac gtgctgctgc gcttctgcgc gctgccgcac
     3061 ctgcacgtgg ctcccgacta cgagcacctg accaaggccg aggactacgc cgaggtcccg
     3121 tggtggctcg ccgaggagta ccccgagctg ttctcgcatc cccgccagct cgaccgcgtc
     3181 cggctctaca gcatcgcctg ggacatccgg gagctgctgg cgttcccacc gtcggaaccg
     3241 ctcaaccgcc tgcacaagca ccacccctac cagcggctct cccagaacct ccgcggcacg
     3301 cactacatcg accgcttcaa cggcaaagcc tccgtccact actgaacggc atcgcctccg
     3361 ccgggtggtg gctggcagga tcgggggatg ggcgacgaac caggaacccc cggtgaaggc
     3421 gtggccgccc ggatccgtgc ggcgtcgtcc acgctgagcc cagcgcagcg ccgcgtcgcc
     3481 gacgccgtgc tggccgaccc gcagctcgtc gccttcggga cggtggccga cctggccgag
     3541 gaggccggcg ccagcggggc gtcggtcgtc cgcctggcca cccggctcgg ctaccacggc
     3601 ttcgtcggca tgcaggaggc ggcccagggc gagctgtccg atcggctccg ccccgcggcc
     3661 agccggatcc gcgacctgcc acccggcgac gtggtcgccc gggcgctgac cacgtcgatg
     3721 caggcggtgg aggccacgct cgccgggctc gaccggagcg tcttcggcca cgccgtcgac
     3781 ctcctcgccg acccggagcg cagcgtggcg gtgatcgccg gcaccgcggg cgccggcatc
     3841 gccgggcacg ccggcgacga gctccgcatg gtgcggtcgg gcgtggaggt ggtgagcggc
     3901 acgccggtcg cggtcgggca gtcgatcgcc cggctcgggc gcggtgacgt cgtgctcgcc
     3961 ttggacctcc gtcgctacga ccactggctg ctcgacgccg tcgaccacgc ggtgcgggag
     4021 ggagcggcgc tcgtcgccct caccgacggg ccggcgtcgc cgctcgcccg acgggcgcgg
     4081 gtcctgctgg tggtcgaagg agaggggatc g
//
LOCUS       DATMFS010000513         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_17919, whole genome shotgun sequence.
ACCESSION   DATMFS010000513 DATMFS010000000
VERSION     DATMFS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17919"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VNQ33_09690"
     CDS             <1..1156
                     /locus_tag="VNQ33_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWJ98420.1"
                     /translation="LDPLELLDQIDEKIGVTAAPVTWPVGIAGDFRGVIDRRTDQFIR
                     FTRTARGATEAPEEVVDADRAGTEEGEAWAAAAEGDEMLSAMGLDYDEELFLGGELSP
                     TFFGSAITNFGVRLLLDGVIDLAPAPSPRFDVDGQPRLLDAPFSGFVFKVQANMDKAH
                     RDRSAFMRVCSGQFERGMVVTHDATGKPFATKYAHTVNGQDRETLEVAYPGDVVGLVN
                     AMDVRVGDTLWLDQTVTFPRIPSFAPEHFSVVRVRDTSKFKQFRKGIAQLDEEGVVQV
                     LRDPDFGDQAPVLAAVGPMQFEVAVHRLENEFGAPVELSPTAYSVARRTDDASAAALR
                     AMRGVDVLDRSDGTRIAVFESTYWLDRLMADQPELVLEPLVAGELAATNG"
     gene            complement(1223..>2561)
                     /locus_tag="VNQ33_09695"
     CDS             complement(1223..>2561)
                     /locus_tag="VNQ33_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98421.1"
                     /translation="WSDCGGGFECGTIALPLDYSKPDGETIDVAVTRHRADNPGERIG
                     TLFVNPGGPGGSAIELARSFGRAGEIGDRFDIVGFDPRGVGESSPLDCHSHLIDIYDA
                     DPSIDSAADREETLATSQAFVDECEQKYGDILPFLGTTDVARDMDEIRKAMGEEQVNY
                     LGYSYGTSLGQEYARLFPTHVRSMVLDGVVDHKPDGLTTAETQAAGFETALDAYIAHC
                     KKDGCGFGAQDAGAVIDAVSAAAEKEPIPAPSADRDAGPGVVSLALAQALYAEQLWGQ
                     LSRALRDAQDRDGSALVALANMYLGTDGGSGISLEAYFAVSCLDDTWPTDPNEVIDAS
                     IQAEKEVPRFGGPIVVDYVRCAMWPAKAKPLKPVPADTDGLPPILVVSTTNDPATPYE
                     NGVAVAEQIPGAVLVTNEGEGHTIVKQGKPCIDDLVDAYLIDLTVPEDGVTCR"
BASE COUNT          336 a          906 c          924 g          395 t
ORIGIN      
        1 cctcgacccg ttggagctgc tcgaccagat cgacgagaag atcggcgtca ccgccgcccc
       61 ggtcacgtgg ccggtgggca tcgccggcga cttccgcggc gtcatcgacc gccggaccga
      121 ccagttcatc cgcttcaccc gcacggcccg cggcgcgacc gaggcgcccg aggaggtcgt
      181 cgacgccgac cgtgccggca ccgaggaggg cgaggcctgg gccgcggccg ccgagggcga
      241 cgagatgctc agcgcgatgg ggctcgacta cgacgaggag ctgttcctcg gcggcgagct
      301 ctcgcccacc ttcttcgggt cggccatcac caacttcggg gtgcgcctcc tgctcgacgg
      361 cgtcatcgac ctcgctccgg ccccctcccc ccgcttcgac gtcgacggcc agccgcggtt
      421 gctcgacgcg ccgttcagcg ggttcgtgtt caaggtccag gccaacatgg acaaggccca
      481 ccgcgaccgc agcgcgttca tgcgggtgtg ctcgggccag ttcgagcgcg ggatggtcgt
      541 gacccacgac gccaccggca agccgttcgc caccaagtac gcccacaccg tcaacggcca
      601 agaccgcgag acgctggagg tcgcctaccc gggcgacgtc gtgggcctgg tcaacgccat
      661 ggacgtccgc gtcggcgaca cgctgtggct cgaccagacg gtgacgttcc cccggatccc
      721 gagcttcgcc cccgagcact tctcggtggt ccgcgtgcgt gacaccagca agttcaagca
      781 gttccgcaag ggcatcgccc agctcgacga ggagggcgtg gtgcaggtgc tgcgcgaccc
      841 ggacttcggt gatcaggccc cggtgctcgc ggccgtcggg ccgatgcagt tcgaggtggc
      901 cgtccaccgc ctggagaacg agttcggggc cccggtcgag ctcagcccca ccgcctactc
      961 ggtggcccgc cgcaccgacg acgccagcgc cgccgctctg cgggccatgc gcggcgtcga
     1021 cgtgctcgac cgctccgacg gcacccgcat cgccgtgttc gagagcacct actggctcga
     1081 ccgcctcatg gccgaccagc ccgagctcgt cctggagccc ctcgtcgccg gtgagctggc
     1141 cgccaccaac ggttgaccgg cgtggcggcg gggcggcgga gcatcgctgg tcaggtcgct
     1201 gccgggccgc cgcggttccc gatcacctgc aggtgacgcc gtcctccggg acggtgaggt
     1261 cgatgaggta ggcgtcgacc aggtcgtcga tgcagggctt gccctgcttg acgatggtgt
     1321 ggccctcgcc ctcgttggtg acgagcacgg cgccggggat ctgctcggcc acggcgacgc
     1381 cgttctcgta gggcgtcgcc gggtcgttgg tggtgctgac cacgaggatc ggcggcaggc
     1441 cgtcggtgtc ggccggtacc ggcttcagcg gcttggcctt cgccggccac atcgcgcagc
     1501 gcacgtagtc cacgacgatc gggcccccga agcgcggcac ctccttctcc gcctggatcg
     1561 aagcgtcgat gacctcgttc gggtccgtcg gccacgtgtc atcgaggcac gacaccgcga
     1621 agtacgcctc gaggctgatc ccgctgccgc cgtcggtgcc gaggtacatg ttcgccaagg
     1681 cgaccagcgc gctgccgtcg cggtcttggg cgtcgcgcag ggcgcgggac agctgccccc
     1741 agagctgttc ggcgtagagc gcctgggcca gggcgagcga gacgacgcct ggtccggcgt
     1801 cgcggtcggc gctcggcgcc gggatcggct ccttctcggc ggccgcgctg accgcgtcga
     1861 tcacggcgcc ggcgtcttgg gcgccgaacc cgcagccgtc cttcttgcag tgggcgatgt
     1921 acgcgtcgag cgcggtctcg aacccggccg cttgggtctc cgccgtggtc agcccgtccg
     1981 gcttgtggtc gacgacgccg tcgaggacca tcgaccggac gtgggtgggg aacaggcggg
     2041 cgtactcctg gccgagcgag gtgccgtacg agtagccgag gtagttgacc tgctcctcgc
     2101 ccatggcctt gcggatctcg tccatgtccc gggcgacgtc ggtggtgccg aggaacggga
     2161 ggatgtcgcc gtacttctgc tcgcactcgt cgacgaacgc ctgcgaggtg gccagcgtct
     2221 cctcgcggtc ggcggcgctg tcgatgctgg ggtcggcgtc gtagatgtcg atcaggtgcg
     2281 agtggcagtc gagcggtgag ctctcgccga cgccgcgggg gtcgaagccg acgatgtcga
     2341 agcggtcgcc gatctccccg gcacggccga acgatcgggc cagttcgatg gccgagccgc
     2401 cgggcccgcc cgggttcacg aacagggtgc cgatgcgctc gccggggttg tcggcgcggt
     2461 ggcgggtgac ggcgacgtcg atggtctcgc cgtcgggctt cgagtagtcg agcgggagcg
     2521 cgatggtccc gcactcgaag ccgccgccgc agtccgacca g
//
LOCUS       DATMFS010000514         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_343846, whole genome shotgun sequence.
ACCESSION   DATMFS010000514 DATMFS010000000
VERSION     DATMFS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343846"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..764)
                     /locus_tag="VNQ33_09700"
     CDS             complement(228..764)
                     /locus_tag="VNQ33_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98422.1"
                     /translation="MAARLRDAPRLRTDEVAELVLTLAEHRDRRVIAPLIDLIASRRV
                     DELTVRAAGWMADPGLHPALVQLRATRIGDLGDAGYWEQVDRAIGRCRPGAADAAEEV
                     EISLLTAAQTSLLESGAAGVELILAGEYPVTEVVLRAGGWERRHAIWNFDETSPDDPA
                     TIDRAFALYRIANLATWG"
     gene            complement(794..1567)
                     /locus_tag="VNQ33_09705"
     CDS             complement(794..1567)
                     /locus_tag="VNQ33_09705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWJ98423.1"
                     /translation="MPEPTIRSEVVGGVGVVTLDAPARRNAFDLAMCDEAVAAVDALE
                     ADPSVGALIVTGAGSAFCAGADLSHLGAASDGSAREGLLAVYEGFLRFARSPLPTIAA
                     VNGAAVGAGMNLALACDVRLAGESARFDTRFLELGIHPGGGHTWMMQNIVGPQATAAT
                     VLFGEVLSGADAERAGLAWRCVPDDELLPLALALAGRAAAAPRELVERVKATIGDAAA
                     ITEHVEAVERELGDQVWSIGQPAFAEKLAALQAKISTPR"
     gene            complement(1560..2303)
                     /locus_tag="VNQ33_09710"
     CDS             complement(1560..2303)
                     /locus_tag="VNQ33_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98424.1"
                     /translation="MSQREVTSARVQGFALGAGSAAVAGGAFAALVHPSDWTWLAGAV
                     AEGAVTAALLVGLARHLRLAEHDVDRVLEAEASEERACSERAGALQAIADSCPRPNEL
                     GKLGPGNIGALDGLGAVPSMPNGKPDFDQWYPRAAPLVVDHLHALTDRLDRAANLWAG
                     ETGAHLRAVRDLNVALTQRWRGWQSADRQRRPRLAELRTHVEAMRHHFDRRDPAQAGP
                     GSTNLPGLVARLEDLAAERRPAVPDSADA"
     gene            complement(2362..>2811)
                     /locus_tag="VNQ33_09715"
     CDS             complement(2362..>2811)
                     /locus_tag="VNQ33_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98425.1"
                     /translation="DPRFQLVHEDDVVAALLFAIDREVPGIYNVAGDGQIPWSEVMAI
                     AGKRGIPMPPFGIALATDPLQRLGLVDFAPEVIELLKYGRGIDNRAFQDAGFAYEHTS
                     AGAVEAFVQAMRLRDTVGSDEINYRYEEDVEKFFRHSPAVQRDRRTT"
BASE COUNT          392 a          965 c         1066 g          388 t
ORIGIN      
        1 agatccagcc tctcgttgac gagacgttcg aacgttaggc aagccggttc tagtcgccag
       61 tgccgatggc tctcatacca ggttacgaac tgttcgttcg tgaatgggag tggagtatca
      121 gaaacactcg tatcgctatc agtcgccgcc atcttcccgc actcccctcg tcggacgacg
      181 aacgatacag ccctgtgact ccatccgccg atgtcgccgg tgggcggtca gccccaggtg
      241 gcgaggttgg cgatgcggta gagggcgaag gcgcggtcga tggtggcggg gtcgtcgggc
      301 gacgtctcgt cgaagttcca gatggcgtgg cggcgctccc acccgccagc gcggagaacg
      361 acctcggtca ccgggtactc gccagcgagg atcagctcga cgccggcggc accggactcc
      421 aggagcgagg tctgcgcggc ggtgaggagc gagatctcca cctcctcggc ggcgtcggcg
      481 gcgccgggcc ggcagcgccc gatcgcccgg tcgacctgct cccagtaccc ggcgtcgccg
      541 aggtcgccga tgcgggtcgc tcgaagctgg acgagtgccg ggtggaggcc gggatcggcc
      601 atccacccgg cggcgcggac ggtcagctcg tcgactcggc gcgatgcgat caggtcgatg
      661 agcggcgcga tgacccgccg gtcccggtgc tcggcgagcg tcagcaccag ttccgccacc
      721 tcgtcggtgc gcagccgtgg cgcgtcccgc agccgcgcgg ccatcgcatc gagatcggga
      781 gcgccctcca cggtcagcga ggcgtcgaga tcttggcttg gagcgcggcg agcttctcgg
      841 cgaaggcggg ctggccgatg gaccacacct ggtcgcccag ctcgcgctcg accgcctcga
      901 cgtgctcggt gatcgcggcg gcatcgccga tggtggcctt cacccgctcg accagctcac
      961 gaggtgcggc ggcggcccgg ccggcgagcg ccagggcgag cgggagcagc tcgtcgtcgg
     1021 ggacgcagcg ccaagccagg ccggcgcgct cggcgtcggc tcccgacagc acctccccga
     1081 acaggacggt ggcggcggtg gcctgcgggc cgacgatgtt ctgcatcatc caggtgtggc
     1141 cgccgccggg gtggatgccg agctcgagga agcgggtgtc gaaccgggcc gactcgccgg
     1201 cgaggcggac gtcgcacgcc agcgccaggt tcatcccggc gccgacggct gcgccgttga
     1261 cggcggcgat ggtgggcagc ggcgagcggg cgaagcggag gaagccctcg tagacggcca
     1321 ggagcccttc tcgggccgaa ccgtccgacg cggcaccgag gtgcgacagg tcggcgcccg
     1381 cgcagaacgc cgagcctgcg ccggtgacga tgagcgcgcc caccgacgga tccgcttcga
     1441 gggcgtcgac cgccgcgacc gcttcgtcgc acatggccag gtcgaacgca ttgcggcgag
     1501 cgggcgcgtc gagcgtgacg acgccgaccc cgccgacgac ctcggaccgg atcgtgggct
     1561 caggcatccg ccgagtctgg caccgctggt cgacgttcgg cggcgaggtc ctcgaggcgg
     1621 gcgacgagcc cggggaggtt cgtggagcct ggacccgcct gggcaggatc gcgccgatcg
     1681 aagtggtggc gcatcgcttc gacgtgcgtc cgcagttcgg ccaggcgcgg ccgccgctgc
     1741 cggtcggcgc tctgccagcc gcgccatcgc tgcgtcagcg cgacgttgag gtcgcgcacc
     1801 gcgcgcaggt gtgctcccgt ctccccggcc cagaggttcg cagcccggtc cagccgatcc
     1861 gtcagggcgt ggaggtggtc gacgacgagc ggcgcggctc gtgggtacca ctggtcgaag
     1921 tcgggcttgc cgttcggcat cgacgggacg gcgccgagcc catcgagggc gccgatgttg
     1981 cccgggccga gcttgccgag ctcgttcggc cgagggcagg agtccgcgat ggcctggagc
     2041 gcgccggcgc gctcggagca ggctcgctcc tcggaggctt ccgcttcgag gacccggtcg
     2101 acatcgtgct cggcgagccg aaggtggcgc gccaggccga cgagcagcgc cgcggtcacg
     2161 gcaccttccg cgaccgcgcc cgccagccac gtccaatccg aagggtggac gagggccgcg
     2221 aaggcccctc cggcgacggc ggcgctcccc gccccgaggg cgaacccctg gacccgggct
     2281 gacgtgacct ctcgttgcga cacgggaggg gagaccccgg cgacgggcga acgatgacgg
     2341 gcgcccgggg atttctgcgg gtcaggtggt gcgccggtcg cgctggacgg cgggggagtg
     2401 gcggaagaac ttctccacgt cctcctcgta ccggtagttg atctcgtcgc tgccgacggt
     2461 gtctcggagg cgcatcgcct ggacgaacgc ctcgacggcc ccggccgacg tgtgctcgta
     2521 ggcgaacccg gcgtcctgga acgcccggtt gtcgatgccg cggccgtact tgaggagctc
     2581 gatcacctcg ggggcgaagt cgaccagccc gaggcgctgc agcggatcgg tggcgagggc
     2641 gatgccgaac ggcggcatcg ggatgccccg cttgccggcg atggccatga cctcgctcca
     2701 ggggatctgc ccgtcgccgg ccacgttgta gatgccgggc acctcgcggt cgatggcgaa
     2761 gagcagggcg gcgacgacgt cgtcctcgtg gaccagctgg aaccgcgggt c
//
LOCUS       DATMFS010000515         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_134002, whole genome shotgun sequence.
ACCESSION   DATMFS010000515 DATMFS010000000
VERSION     DATMFS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134002"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VNQ33_09720"
     CDS             <1..790
                     /locus_tag="VNQ33_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWJ98426.1"
                     /translation="EFIESIRLNTSAEFKRRYREVDVLLVDDIQFLENRKETQEEFFH
                     TFNALHEGRRQLVLSSDRPPDAISTLENRLRSRFKMGLITDIQPPDLETRLAVLGKKA
                     ENEGFPVPDDVLEFIAVNITDNIRELEGALNRVSALAKLHKRPITLPEAEKHLHDIIG
                     DRQPRPITPDLILQATSDMFGFTIEEIKGKSRQRPLVTARQIAMYVFRDSTDLSYPAI
                     AREFGGRDHTTVIHAVEKIKALMGERRQIYDQVTELLNQIRKGS"
     gene            1105..2202
                     /gene="dnaN"
                     /locus_tag="VNQ33_09725"
     CDS             1105..2202
                     /gene="dnaN"
                     /locus_tag="VNQ33_09725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWJ98427.1"
                     /translation="MKFRCERDTLVEALASAGRAVANRGGALPVLSGVRAELTGDNLR
                     LTGSDLELTIEIEITVAGERDGVAVLPAKISSDLVRSLGDPRVEVAVEGDEARITAGR
                     FESSLRLLPADEFPRLAMPADDAVTLAAKDFAGALRQVVPAASADDARPILTGVLLAA
                     EAGGLRLVATDSYRLAVRDLPGTSVLGEGQSVLVPSRALRELERLLGAAEEVTLRLGE
                     REAAFEVGDVRLTTRLIEGEFPNYRGLIPASHPNRLTVGREALVEAVKRVKLMARELN
                     TPVRLAMSSDGLELVAITQDVGQAHEQLDASYEGTELTVAFNPEYLLSGVEVTPGDEI
                     HLDTVDAQKPAVIRAGEAQEFLYLLMPVRVS"
     gene            2220..>2546
                     /locus_tag="VNQ33_09730"
     CDS             2220..>2546
                     /locus_tag="VNQ33_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ98428.1"
                     /translation="MQLRRLWLTDFRGYREADVAFDPGLTAVLGPNGQGKTNLLEAIG
                     YLATLSSFRGAPTEALVRSGADRAVVRAEAEREGRELLLEAELVANGRNRTQLNKQPL
                     RRARDLL"
BASE COUNT          407 a          928 c          836 g          375 t
ORIGIN      
        1 cgagttcatc gagtccatcc ggctcaacac ctcggccgag ttcaagcgcc gctaccgcga
       61 ggtcgacgtc ttgttggtgg acgacatcca gttcctggag aaccgcaagg agacccagga
      121 ggagttcttc cacacgttca acgccctcca cgagggtcgc cgccagctcg tgttgtcgtc
      181 cgaccgaccg cccgatgcca tctccacctt ggagaaccgg ctccgcagcc ggttcaagat
      241 gggcctgatc accgacatcc aaccgcccga cctcgagacg cgcctcgcgg tgctcgggaa
      301 gaaggccgag aacgagggct tcccggtgcc cgacgacgtg ctcgagttca tcgccgtcaa
      361 catcaccgac aacatccgcg agctcgaggg cgccctcaac cgcgtctccg cgctggcgaa
      421 gctccacaag cgccccatca cgctgcccga ggccgagaag cacctccacg acatcatcgg
      481 cgaccgccag ccccggccga tcaccccgga cctgatcctc caggcgacct cggacatgtt
      541 cgggttcacc atcgaggaga tcaagggcaa gagccgccag cgcccgctgg tcaccgcccg
      601 ccagatcgcc atgtacgtgt tccgggactc gaccgacctg tcgtacccgg ccatcgcacg
      661 cgagttcggc gggcgcgacc acacgacggt gatccacgcc gtggagaaga tcaaggccct
      721 gatgggggag cgccgacaga tctacgacca ggtcaccgag ctgctcaacc agatccggaa
      781 gggaagttga gcatgcgccg cgcgtgccgg tcgtcacacg agttgtccac cgttgtccac
      841 agggacctgg gcatttctcc gtccatcacg ctggatgcag ctgtggacgg agccggggac
      901 ggcaggggac gcgcggggac aagtcatgct ccggacacgt cgcatgcacc gcggcgcccc
      961 gaacctgtgg aacgctgtgg attccccccg agatgccgaa tgcctgctga cctgcactga
     1021 tggacgctta tccccagtcc acaggtccca ccaccaccat cagattctga tcagcacctt
     1081 ctctggagaa cgtgaggtta cgaagtgaag ttccgttgcg agcgtgacac cttggtcgag
     1141 gccctggcct cagccggccg tgcggtggcc aaccgcggcg gcgccctgcc cgtgctctcg
     1201 ggcgtccgcg ccgagctcac gggcgacaac ctccgtctca ccggcagcga tctcgagctc
     1261 accatcgaga tcgagatcac ggtggccggc gagcgtgacg gtgtcgccgt gctgcccgcc
     1321 aagatctcct cggatctcgt ccggtcgctg ggtgaccctc gcgtggaggt ggcggtcgag
     1381 ggcgacgagg cgcgcatcac ggctggacgc ttcgagtcct ccctgcggct gctgcccgcc
     1441 gacgagttcc cccggctggc gatgcccgcc gacgacgcgg tgacgctggc cgccaaggac
     1501 ttcgccgggg ccctgcgcca ggtggtgccc gcggccagcg ccgacgacgc ccgcccgatc
     1561 ctcaccggcg tgctcctcgc ggccgaggcc ggcggtctgc gcctcgtcgc caccgactcc
     1621 taccgcctcg ccgtgcggga cctgccgggc acctcggtgc tgggcgaggg ccagagcgtc
     1681 ctcgtgccct cccgggccct gcgcgagctg gagcggctcc tgggcgctgc cgaggaggtc
     1741 acgctgcggc tgggggagcg cgaggcggcg ttcgaggtcg gcgacgtccg gttgacgacc
     1801 cgcctgatcg aaggcgagtt ccccaactac cgcggcctca tccccgcgag ccacccgaac
     1861 cggctcaccg tgggccgtga ggcgctggtc gaggccgtga agcgcgtgaa gctcatggca
     1921 cgcgagctga acacgccggt gcggctggcc atgagcagcg acggcttgga gctggtcgcc
     1981 atcacccaag acgtcggtca ggctcacgag cagctcgatg cctcctacga gggcaccgag
     2041 ctcaccgtgg cgttcaaccc ggagtacctc ctgagcggcg tcgaggtgac ccccggagac
     2101 gagatccacc tcgacacggt cgacgcccag aagccggccg tcatccgcgc cggcgaggcc
     2161 caggagttcc tgtacctgct gatgccggtg cgggtctcct gaccgccgcg gcctgagcgg
     2221 tgcagctgcg gcgcctctgg ctgaccgact tccgcggcta ccgcgaggcc gacgtcgcgt
     2281 tcgaccctgg actgaccgcc gtgctcggtc ccaacggcca gggcaagacc aacctgctcg
     2341 aggccatcgg ctacctggcc acgctctcgt cgttccgggg cgcgcccacc gaggcgctgg
     2401 ttcgatccgg tgccgatcgg gcggtggtcc gggccgaggc ggagcgcgag ggccgtgagc
     2461 tgctcctcga ggccgagctg gtggccaacg gccgcaaccg cacccagctc aacaagcagc
     2521 cgctgcgccg ggcccgcgat ctgctc
//
LOCUS       DATMFS010000516         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_63431, whole genome shotgun sequence.
ACCESSION   DATMFS010000516 DATMFS010000000
VERSION     DATMFS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63431"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VNQ33_09735"
     CDS             complement(<1..578)
                     /locus_tag="VNQ33_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98429.1"
                     /translation="MSEDLREGEGGAADGVRGDDVGPHQLLTAPTGRRTWTPTFFGVV
                     PAGSVRRRPSDALHLAIGAVVLAACYVATDGFRPRSDRFFHWLNGLPGWVDAIGTTLF
                     VASTLGAVVVVVGSLLLSRNSRLAVTLVVLAAATGALAWVIAGVIELDQVRRAAGGDA
                     QRFTESPVWLAVAVAVLLAVAPYLVRPARRSV"
     gene            964..1034
                     /locus_tag="VNQ33_09740"
     tRNA            964..1034
                     /locus_tag="VNQ33_09740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:996..998,aa:Gly,seq:ccc)
     gene            complement(1140..2330)
                     /locus_tag="VNQ33_09745"
     CDS             complement(1140..2330)
                     /locus_tag="VNQ33_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HWJ98430.1"
                     /translation="MSSLQGSPVGSVVPIAGFDARFDEEYRTAAFVPADLPDRIDLPG
                     PVWMVVSEANAELGRLDAAASLIPNPQLVTRIATRREAVGTSALEGTYAELTELFAAE
                     VLPFEEQETEVAPNVREVMNYARAADAAYGWIGERPITLGMLASLQAEIVRGTDSDGP
                     EAGSIRQSQVFIGAKHRRVAEARFVPPPPGDQLRAMCERWVDWLSAAEPVEQIQLIAR
                     VAMAHYQFETLHPFIDGNGRVGRLVAVLQLMQAGALRSPVLSVSPWLKDRADAYRDHL
                     LAVSTTGDWAPWIEFFAQAIRAEARSGHERIMRLLALREQLGDVVRSALPRARLALEI
                     ADDLIAYPILSVSDAQRRYGRSNQANRNAIGSLIELGVLEPYSDAPYDRLYWNRRVFQ
                     IIDS"
     gene            complement(2530..3615)
                     /locus_tag="VNQ33_09750"
     CDS             complement(2530..3615)
                     /locus_tag="VNQ33_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HWJ98431.1"
                     /translation="MADGDLLPRSYREARAAFLEAAEAAGARITSAVHPLRGIDDEEL
                     AIDVAEHGPADADDVVVVVSGTHGVEGYLGSALQTQHLRLLADGSTGGGDGPGLVFIH
                     ALNPYGFSWVRRVNEDNVDLNRNFIDWTAPLPVANVEYAEMADTFVPGDWSVETQERT
                     LLEMAAKLEEVGAAQLQQIVSGGQYSHPTGVFYGGAKAVWSNDWLQGFLGGRLAGVRR
                     AAIIDLHTGLGPWGHGELISSEKPDSEAYARQTGWWGEVTSLHDGSSVSAELVGDWLA
                     ALPSFVVGPEITGIAIEYGTVDSITVLQSLRADAVLHATGDPIAPGAVAIRDQVRAAF
                     LDDDPTWLATCADRYRSVVKATLAALA"
     gene            complement(3650..>3895)
                     /locus_tag="VNQ33_09755"
     CDS             complement(3650..>3895)
                     /locus_tag="VNQ33_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98432.1"
                     /translation="RAADLVAPGGVLLSVSHAAAPPWSDHHDHVFPTTDEELAELALD
                     PVDWEVVRNGDVEREATGPDGQHGCLLDGVILVRRRP"
BASE COUNT          613 a         1366 c         1370 g          546 t
ORIGIN      
        1 cacacgctgc ggcgggccgg acgcacgagg tacggcgcca ccgccagcag cacggccacg
       61 gcgacggcga gccacaccgg gctctcggtg aaccgctggg catctccgcc agccgcacgc
      121 cggacctgat cgagctcgat cacaccagcg atcacccagg cgagggcgcc ggtggccgcc
      181 gcgagcacga ccagggtcac ggccagtcgg ctgttgcggg agagcagcag cgacccgacg
      241 accacgacga ccgcgccgag ggtcgaggcc acgaacagcg tcgtgccgat ggcgtcgacc
      301 cacccaggca ggccgttcag ccagtggaag aagcggtcgg accggggcct gaacccgtcg
      361 gtcgccacgt agcaggcggc cagcaccacc gcgccgatgg cgaggtgcag cgcatcgctc
      421 ggacgcctgc gcacgctgcc ggccgggacg acgccgaaga aggtcggcgt ccacgtccgc
      481 cgcccggtcg gggcggtgag aagctggtgc gggccgacgt catcgcctcg gacgccgtcc
      541 gctgcgcctc cttcgccctc ccgcaggtcc tcgctcatgc cgcccgcagc gtacgacagc
      601 gcctcgcccg gccctggggt ggtggcgacc cgtgccgtcc cgcctcctgc gccggactcg
      661 gcgtacggac ggatgcgctc cctcggcacc ctggccggcg gcaagcccgg cgcaccgccg
      721 tgatcgcccg acgcggtggc gacgcgccgt cgtgtccttc ccccgcccga gcacgcgcga
      781 tcgagctggc caccgccccc gggtgcctgt cggcgcccaa cggcgaccgc aacggagacg
      841 gaccgtccgg tgggcgtgct cgccccgctc atctggctgg gcgaaggacg acccgggtcg
      901 ggtagggtca cccctcgctg gatcgccgtc cgaaggcccc cgagccccag gccgacgccc
      961 cacgcgggtg tagttcaatg gcagaacatc agcttcccaa gctgaatacg ggagttcgat
     1021 tctcctcacc cgctctcaag agaaacccac tcggcgagca gccgcgtggc gagttcgacg
     1081 tcgtcctccg cctccgattc acgctccatc agtcggctcc cccacttcca tccgttggat
     1141 caactgtcga tgatctggaa cacccgtcgg ttccagtaga ggcggtcgta gggcgcatcg
     1201 ctgtagggct cgagaacgcc gagctcgatg agcgatccga ttgcattgcg gttggcctgg
     1261 ttcgagcggc cgtagcgccg ttgagcgtcc gagaccgaga ggatcgggta ggcgatgagg
     1321 tcgtcagcga tctccaaggc gagccgcgcc cgcggaagcg cagaacgcac gacgtcaccg
     1381 agctgttcac gcaaggcgag gaggcgcatg atgcgctcgt ggccagaccg agcttcggcg
     1441 cggatggctt gggcgaagaa ctcgatccaa ggcgcccagt ccccggtggt gctcaccgcc
     1501 agcaggtgat cgcggtacgc gtcggcacga tccttcagcc agggcgacac cgacaggacc
     1561 ggggaccgaa gcgcgcccgc ttgcatcagc tgcaggacag cgacgagacg gccgaccctg
     1621 ccgttgccat cgatgaacgg gtggagcgtc tcgaactggt agtgcgccat ggccacgcga
     1681 gcgatgagct ggatctgctc gactggctcg gcagcgctga gccagtcgac ccaccgctcg
     1741 cacatggcgc gaagctggtc ccctggggga ggcggaacga accgtgcctc ggccacccgg
     1801 cgatgcttcg cgccgatgaa cacctggctc tggcggatcg agccggcctc cggcccgtcg
     1861 ctgtctgtgc ctcgaacgat ctcggcctgc aatgacgcca acatccccaa cgtgatcggg
     1921 cgctcgccga tccagccgta cgccgcgtcc gcagcacggg cgtagttcat gacctcgcgc
     1981 acgttgggcg cgacctcggt ctcctgctcc tcgaacggga gcacctcggc ggcgaacaac
     2041 tcggtgagct cggcgtaggt tccctccagc gccgaggtgc cgaccgcctc acggcgggtc
     2101 gcgatgcgag tcaccagctg ggggttcggg atgaggctcg ccgctgcatc aaggcgaccc
     2161 agctcagcgt tggcctcgct gaccaccatc cagacgggtc ccggcaggtc gatgcgatca
     2221 ggaagatcgg cagggacgaa cgccgcagtt cggtactcct catcgaaccg agcgtcgaac
     2281 ccggcgatgg gaacgaccga cccgaccggc gagccctgca acgacgacat gacttggaat
     2341 gtaccgccac gattccaagt ttcgcgtgcg aaacttggaa ttacgtgggt ggaatccaag
     2401 tctcggtcag gcgctccgga cagaggtctc cagcctcctg ggccttcggc gttccgggcc
     2461 ggcatcggag gcgtgacctc cccctcgcct ctcgtcagcg tcgaggttgt gagctgaggt
     2521 gcgcggcgct caggccaggg cggcgagggt ggccttcacc accgaccggt agcggtcggc
     2581 gcaggtggcg agccaggtcg ggtcgtcgtc gaggaacgcg gcccgcacct ggtcgcggat
     2641 ggcgaccgct ccgggcgcga tcgggtcgcc ggtggcgtgg aggacggcgt cggcccgcag
     2701 gctctggagc acggtgatcg agtcgacggt gccgtactcg atggcgatgc cggtgatctc
     2761 aggaccgacc acgaagctgg gcagcgcggc gagccagtcg ccgaccagct cggccgagac
     2821 gctggagccg tcgtgcagcg aggtcacttc gccccaccac ccggtctggc gggcgtaggc
     2881 ctcgctgtcg ggcttctcgc tggagatcag ctcgccgtga ccccacggac cgaggccggt
     2941 gtggaggtcg atgatcgcag cccggcggac gccggcaagc cgcccgccca agaacccctg
     3001 cagccagtcg ttcgaccaca ccgccttggc tccgccgtag aacacgcccg tcggatggct
     3061 gtactgccca cccgagacga tctgctggag ctgggcggcg ccgacctcct cgagcttcgc
     3121 cgccatctcg agcagcgtgc gctcctgcgt ctcgaccgac cagtcgccgg ggacgaacgt
     3181 gtcggccatc tcggcgtact cgacgttggc cacgggaagc ggcgcggtcc agtcgatgaa
     3241 gttgcggttc aggtcgacgt tgtcctcgtt gacccgccgc acccacgaga agccgtacgg
     3301 gttgagggcg tggatgaaca cgagtcccgg gccgtccccg ccaccggtgc tgccatcggc
     3361 gagcagccgc aggtgctggg tctggagggc ggagccgagg tagccctcga cgccgtgggt
     3421 ccccgacacg acgaccacga cgtcgtcggc gtcggccgga ccgtgctcgg cgacgtcgat
     3481 ggccagctcc tcgtcgtcga tcccccgcag ggggtgcacc gccgacgtga tccgggcgcc
     3541 agcggcctca gcggcctcca ggaacgcagc ccgggcctcg cggtacgaac gggggaggag
     3601 atcgccgtca gccatggaac gaagtcttgc agggacgatg gcaaggcgtt cagggccggc
     3661 ggcggacgag gatgacgccg tcgagcaggc agccgtgctg gccgtcggga ccggtcgcct
     3721 ctcgctcgac gtcgccgttt cggacgacct cccaatcgac tggatccagg gccagctcgg
     3781 ccagctcctc gtcggtggtg gggaagacgt ggtcgtggtg gtcggaccag ggcggggcgg
     3841 cggcatggct caccgagagg aggacgccgc cgggggcgac gaggtctgcg gcccg
//
LOCUS       DATMFS010000517         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_41796, whole genome shotgun sequence.
ACCESSION   DATMFS010000517 DATMFS010000000
VERSION     DATMFS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41796"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VNQ33_09760"
     CDS             <1..1366
                     /locus_tag="VNQ33_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98433.1"
                     /translation="LDGFAAECRADGLTDAVVMGMGGSSLFPEVVARSFPTGERGLRL
                     HVLDSTDPAAVGRIAAHLHPDTTLYLASSKSGSTIETRSHLDFFWERVGRGDRFAVVT
                     DPGSALGALARERGFRQVFENRPDIGGRYSALSLFGLVPAALAGIDWLGLVAAAQRVE
                     ASLRDPDPTVNVGLRLGAALAAGVKAGRDKATLVIDPRVETFGLWLEQLLAESTGKQG
                     TGVVPIAGEALGPVDVYGTDRIFVSIGDVEHPVGLDVLADEGNPVIELDLDDPLDLGA
                     HVLVWEVATALCGAALGINAFDQPNVAEAKEATAKVLAEGAGDLPSGRLAEVLLDLRP
                     GDYLAIQAYLEPADDAVPLLEEIRLELRDEHQVATTFGLGPRFLHSTGQLHKGGPPTG
                     VFLQVVGADPSDAAIPGQQFTFSQLKHAQADGDLLTLAKHGLRACRTTLAELTDHVDK
                     GDVR"
     gene            1363..2289
                     /gene="gnd"
                     /locus_tag="VNQ33_09765"
     CDS             1363..2289
                     /gene="gnd"
                     /locus_tag="VNQ33_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWJ98434.1"
                     /translation="MKLGMIGLGKMGGFMARRLEDGGHEVVGMDPAKPEADAHSLEEL
                     VPLLAAEGQPKVAWSMVPSGPITDGVISELGERLAPGDLVIDGGNSNYLDSIRHGEEL
                     AAKGIGFVDCGTSGGVWGLQNGYALMVGGTDESVAIAQPLFDALAPPSGKGFAHAGPV
                     GTGHYTKMVHNGIEYGIMQAYAEGYAILDASPLGIDVQKAIGSWQEGSVVRSWLLDLL
                     IKALEETPDLDGIASVAADSGEGRWTVTEAVANGVAAPVISAALFARFTSQHREEDKA
                     MKVISALRNQFGGHAIELEPPKGEQHGGEGQG"
     gene            2286..>2706
                     /locus_tag="VNQ33_09770"
     CDS             2286..>2706
                     /locus_tag="VNQ33_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWJ98435.1"
                     /translation="MTGEQSDALVLFGATGDLAHKKIFPSLYAMALEGQLKVPVIGVA
                     SSAGDDEFLRGKVRASLAEQVADAEDVVVEHLLGQVRYVSGDYREPTVFDELAKVLEP
                     CERPLFYLAIPPTLFDDVLDGLRRVGANEGGRVVVEKP"
BASE COUNT          380 a         1011 c          940 g          375 t
ORIGIN      
        1 gctcgacggc ttcgcagccg agtgccgggc cgacggcctc accgacgcgg tggtcatggg
       61 catgggcggc tcgagcctgt tccccgaggt cgtggcccgt tcgttcccga cgggggagcg
      121 gggtctgcgc ctccacgtgc tcgacagcac cgaccccgcc gcggtgggcc gcatcgccgc
      181 ccacctccac cccgacacca ccttgtacct ggcgtcgtcg aagtcgggtt ccacgatcga
      241 gacccggagc cacctggact tcttctggga gcgggtgggc cgcggcgacc ggttcgccgt
      301 cgtcaccgat ccgggctcgg cgctcggcgc cctcgcccgc gagcggggct tccgccaggt
      361 gttcgagaac cggcccgaca tcgggggccg ctactcggcg ctgagcctct tcggcctggt
      421 gcccgcggcg ctggccggca tcgactggct gggactggtc gccgccgccc agcgggtgga
      481 ggcgtcgctg cgcgatcccg acccgaccgt gaacgtcggc ctccgcctcg gcgccgccct
      541 ggctgcgggc gtcaaagccg gccgggacaa ggcgaccctg gtcatcgacc cccgcgtcga
      601 gaccttcggc ctgtggctcg agcagctgct cgccgagtcc accgggaagc agggcaccgg
      661 ggtcgtcccc atcgccggcg aggccctcgg ccccgtcgac gtgtacggca ccgaccgcat
      721 cttcgtgtcg atcggcgacg tcgagcaccc cgtcggcctc gacgtgctcg ccgacgaggg
      781 caacccggtg atcgagctcg acctcgacga cccgctcgac ctcggtgccc acgtgctcgt
      841 gtgggaggtg gccaccgccc tgtgcggtgc cgccctcggc atcaacgcct tcgaccagcc
      901 caacgtggcc gaggccaagg aggccaccgc caaggtgctg gccgaagggg cgggcgacct
      961 ccccagcggg cggctggccg aggtgctgct ggacctccgt cccggcgact acctcgccat
     1021 ccaggcctac ctggagccgg ccgacgacgc cgtgcccctc ctcgaggaga tccgcctgga
     1081 gctgcgcgac gagcaccagg tcgccaccac gttcgggctc ggcccgcgct tcttgcactc
     1141 caccggccag ctccacaagg gcggcccgcc caccggcgtc ttcctccagg tggtgggcgc
     1201 cgacccgtcc gacgcggcca tcccggggca gcagttcacc ttctcgcagc tcaagcacgc
     1261 ccaagccgac ggagacctgc tcacgctcgc caagcacggg ttgcgcgcct gccgcaccac
     1321 gctcgccgag ctgaccgacc acgtggacaa gggagacgtc cgatgaagct cggcatgatc
     1381 ggtctcggga agatgggcgg cttcatggcc cggcgcctgg aggacggcgg ccacgaggtc
     1441 gtcggcatgg acccggccaa gccggaggcc gacgcccatt cgctagagga gctggtcccg
     1501 ctgctggcgg ccgagggcca gcccaaggtg gcgtggtcga tggtcccgtc gggtccgatc
     1561 accgatggcg tgatctccga gctgggcgag cggctggcgc cgggcgacct cgtcatcgac
     1621 ggcggcaact ccaactacct ggactcgatc cgccacggcg aggagctggc cgcgaagggg
     1681 atcggcttcg tcgactgcgg cacctccggc ggcgtgtggg ggctccagaa cgggtacgcc
     1741 ctgatggtgg gcggcaccga cgagtcggtg gccatcgccc agccgctgtt cgacgcgctg
     1801 gccccgccca gcggcaaggg cttcgcccac gccggcccgg tgggcaccgg ccactacacg
     1861 aagatggtcc acaacggcat cgagtacggg atcatgcagg cctacgccga gggctacgcc
     1921 atcctcgatg cctcgccgct gggcatcgac gtgcagaagg ccatcggcag ctggcaggag
     1981 gggagcgtcg tgcgctcctg gctgctggac ctcctcatca aggcgctgga ggagaccccc
     2041 gacctcgacg gcatcgcgtc ggtggcggcc gactcgggcg agggccgctg gaccgtcacc
     2101 gaggccgtcg ccaacggcgt ggccgccccg gtcatctctg ccgccctctt cgcccgcttc
     2161 acctcgcagc accgcgagga ggacaaggcc atgaaggtca tctcggccct gcgcaaccag
     2221 ttcgggggcc acgccatcga gctcgagcca ccgaagggcg agcagcacgg cggcgagggc
     2281 cagggatgac cggcgagcag tccgacgccc tcgtcctctt cggggcgacc ggagacctcg
     2341 cccacaagaa gatcttcccg tcgctctacg ccatggcgct cgaagggcag ctgaaggtcc
     2401 ccgtgatcgg ggtggcctcg tcggcaggcg acgacgagtt cctccgcggc aaggtccgag
     2461 cctcgctggc cgagcaggtc gccgacgccg aggacgtcgt cgtcgagcac ctgctcggcc
     2521 aggtccgcta cgtgtccggc gactaccgcg agcccaccgt cttcgacgag ctggccaagg
     2581 tgctcgagcc ctgcgagcgc ccgctcttct acctggccat cccgcccacg ctcttcgacg
     2641 acgtgctcga cggcctgcgc cgcgtcggtg ccaacgaagg ggggcgggtc gtcgtcgaga
     2701 agccct
//
LOCUS       DATMFS010000518         5807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_41811, whole genome shotgun sequence.
ACCESSION   DATMFS010000518 DATMFS010000000
VERSION     DATMFS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41811"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /gene="glyA"
                     /locus_tag="VNQ33_09775"
     CDS             <1..1091
                     /gene="glyA"
                     /locus_tag="VNQ33_09775"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWJ98436.1"
                     /translation="EGYPGKRYYGGNEIIDQAEELARERVKALFGAEHANVQPHSGAN
                     ANMAVYLGLLDHGDTVLGLRLDQGGHLTHGSPVNASGILYDFVSYGVTPSDERIDFDQ
                     VRDLALEHRPKIIVAGATAYPRIIDPQPFREIADEVGALFMFDAAHIAGLIAGGVHPN
                     PVPVADIVTFTTHKTLRGPRGGCILSKAEHAAAIDKAVFPGSQGGPLDHVIAAKAVAF
                     REAATPEFTQYAHDVVSNASALAEALASHGFRLVSGGTDNHLMLVDLRPFDADLSGKK
                     ARLVLDRAGISLNENTVPGDERPPYITSGLRIGAPAVTTQGMGHREMREIAFQIHRVL
                     THRDDDAVIAEARESVATLCSKFAPYPG"
     gene            1120..2373
                     /locus_tag="VNQ33_09780"
     CDS             1120..2373
                     /locus_tag="VNQ33_09780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWJ98437.1"
                     /translation="MPTTNAYVAAFVIALVVTFVATPLVKRAAFRLKVVAIPSDRSMH
                     EKPMPLLGGAAMLIGFLAAMGVAWVTGAFPAMFEDQFPTIIGVVVGAVVLCAIGTLDD
                     VRDVSAPAKTAGIVLAASVMYLFGVTMLYFRPPFLDGLFVVPEDLAPLITVLWVLGMA
                     NAVNLIDGLDGLAAGIVAIGSGAFFLYGHHLATAGVQIIEPSNPSPLICVVTAGICLG
                     FLPHNFNPARIFMGDGGALMLGGLMAASTILVGGQTSQPFSGQVFFFFAPLFIPFFVM
                     AIPIIDTAFAIIRRARKRAGLAEADKDHLHHRLMRLGHGQRRSVAILWLWSGLLSLFV
                     LYPVYTGKGNGLVPIAVAALALILLTFFHPGTRRARQQEAAEEEHRRLQQIAEADTEP
                     VGDDAVEVPADDLDPVDATADPGAS"
     gene            2539..2907
                     /locus_tag="VNQ33_09785"
     CDS             2539..2907
                     /locus_tag="VNQ33_09785"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWJ98438.1"
                     /translation="MSDSTSRITGNEAPAPSPAVGVPASSAPRASADPFSRTSYAKSL
                     NRGYGDAMGRGLELAMTLVFMVAIGRLADFVFGTYPICTIVFSILGFAGITVKLFFGY
                     DLEMRKHEENAIWNRKSESA"
     gene            2907..3368
                     /locus_tag="VNQ33_09790"
     CDS             2907..3368
                     /locus_tag="VNQ33_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWJ98439.1"
                     /translation="MATTVRAKPSVIESRDLGPAPEAQVARDLIKRGLIALPFALVLG
                     AIGWGTAGIASVAYAAGLILVNFAVAAALLGWAARVSLGLLMGVCLFGFLIRMAAITA
                     AVLLVRDQSWVAPVPLAVTLIVTHLGLLFWETKFVSASLAFPGLKPSSEEQ"
     gene            3371..4129
                     /gene="atpB"
                     /locus_tag="VNQ33_09795"
     CDS             3371..4129
                     /gene="atpB"
                     /locus_tag="VNQ33_09795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWJ98440.1"
                     /translation="MLALEFPPLSHITNWGGYVGDGEIWEINKIVLVYGFALLFTWLL
                     FFLGRKGEIVPTGAQNLAEMSIEFVEDGIVMDTMGEEGKKYTPFLLSLFLFIFFTNIF
                     EVVPVIQMPASARIAMPMTLAIIAYLVYHGAGFKSHGLGYFKHALVPPGVPIGILPLV
                     VIIEFVSKFLVQPFSHTVRLFANLLAGHILLVTFAVLTSALITPKWYAIFAPLPFAGV
                     VFFTAFEVFVSFLQAYVFTLLTGVYINAAASHEH"
     gene            4182..4424
                     /gene="atpE"
                     /locus_tag="VNQ33_09800"
     CDS             4182..4424
                     /gene="atpE"
                     /locus_tag="VNQ33_09800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWJ98441.1"
                     /translation="MSIAVLAAEGLTNKGVAALTYGLGAIGPGIGIGYLVGQSVQAMA
                     RQPESAGMVRTTMFLGIAFVEALALIGFVVFFLAGA"
     gene            4440..5120
                     /gene="atpF"
                     /locus_tag="VNQ33_09805"
     CDS             4440..5120
                     /gene="atpF"
                     /locus_tag="VNQ33_09805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWJ98442.1"
                     /translation="MKRTRLLLVAAAIVAVGLFGFARPSMAQEASGESTSEVSHSVHE
                     CIELMEDANSIDVECQKAPNPLVPEKNEVIWGFVGFILVFGFLYWKAVPSMKKGMNAR
                     TERIKGDLDAAEAQRAEASEILAQYKAQLADARTESARIIEEARQAADEVRANLQTKA
                     TADIAELRARATADIESAKQQAVADLRGEVTSLAIGAAEQVVGRNLDAETNAALVEAY
                     IDQVGANS"
     gene            5120..5647
                     /gene="atpH"
                     /locus_tag="VNQ33_09810"
     CDS             5120..5647
                     /gene="atpH"
                     /locus_tag="VNQ33_09810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HWJ98443.1"
                     /translation="MADDRTTAYAEALFAVARTEGPLSEVEDELFRVAQVVRGNDELR
                     DKLADPHIPVATRQQIIIDLLSGKAEPTTVSLVSLVVANGRIRELPAIVDELLAMAAA
                     EGNKEVAEIRSAIALTDDQKSRLAEALGKATGKQVEVRVIIDPSIKGGIVAQVGDTVI
                     DGSVRRRLEQLKNAL"
BASE COUNT          878 a         2179 c         1808 g          942 t
ORIGIN      
        1 ccgagggcta ccccggcaag cgctactacg gcggcaacga gatcatcgac caggccgagg
       61 agctggcccg ggagcgggtg aaggcgctgt tcggcgccga gcacgccaac gtccagcccc
      121 actcgggcgc caacgccaac atggccgtgt acctcggcct gctggaccac ggcgacaccg
      181 tgctcggcct ccggctcgat cagggcggcc acctcacgca cggatcgccg gtcaacgcca
      241 gcggcatcct ctacgacttc gtgtcgtacg gcgtgacgcc gtccgacgag cgcatcgact
      301 tcgaccaggt ccgggacctg gcgctcgagc accggccgaa gatcatcgtc gccggcgcca
      361 ccgcctaccc ccggatcatc gacccgcagc cgttccggga gatcgccgac gaggtcggcg
      421 ccctgttcat gttcgatgcc gcccacatcg ccggcctcat cgccggtggc gtgcacccga
      481 acccggtccc ggtcgccgac atcgtcacct tcaccaccca caagacgctg cgcggcccgc
      541 gcggcggctg catcctgtcg aaggccgagc acgccgccgc catcgacaag gcggtgttcc
      601 ccggctcgca gggcggcccg ctcgaccacg tgatcgcggc caaggccgtg gccttccgcg
      661 aggcggccac acccgagttc acccagtacg cccacgacgt cgtgtccaac gcgtcggccc
      721 tggccgaggc gctggcgagc cacggcttcc ggctggtgtc gggcggcacc gacaaccacc
      781 tgatgctcgt cgacctccgc ccgttcgacg ccgacctgtc gggcaagaag gcccgcctgg
      841 tcctcgaccg ggccggcatc agcctcaacg agaacaccgt tcccggcgac gagcgcccgc
      901 cctacatcac gtcgggcctg cgcatcggcg ctccggcggt gaccacccag ggcatgggcc
      961 accgggagat gcgggagatc gccttccaga tccaccgcgt cctcacgcat cgtgacgacg
     1021 acgccgtcat cgccgaggcc cgcgagtcgg tcgccaccct ctgctcgaag ttcgccccct
     1081 accccggctg accgccggcg gcggcgagac tggtccgcga tgcccaccac caacgcctac
     1141 gtggctgcct tcgtcatcgc gctcgtggtg acgttcgtgg ccacgcccct ggtgaagcgg
     1201 gccgcgttcc gtctcaaggt ggtggccatc ccgtcggacc ggtcgatgca cgagaagccc
     1261 atgccgctgc tcggcggggc ggccatgctc atcgggttcc tggcggccat gggggtggcc
     1321 tgggtcacgg gtgcgttccc ggcgatgttc gaggaccagt tccccacgat catcggggtg
     1381 gtggtcggcg ccgtggtcct ctgcgccatc ggcacgctcg acgacgtccg ggacgtgtcg
     1441 gccccggcca agaccgcggg gatcgtgctg gcggcgtcgg tcatgtacct gttcggcgtc
     1501 accatgctct acttccgccc gccgttcctc gacgggctct tcgtggtgcc cgaggacctc
     1561 gctccgctca tcaccgtgct gtgggtgctg ggcatggcca acgccgtgaa cctcatcgac
     1621 gggctcgacg gcctggcggc gggcatcgtc gccatcggct ccggtgcgtt cttcctctac
     1681 ggccaccacc tcgccaccgc aggcgtccag atcatcgagc cgtcgaaccc cagcccgctc
     1741 atctgcgtcg tcaccgccgg catctgcctc gggttcctgc cgcacaactt caacccggcc
     1801 cgcatcttca tgggcgacgg cggggcgctc atgctcggcg gtctcatggc cgcgtcgacg
     1861 atcctcgtcg gcggtcagac cagccagccc ttctcgggcc aggtgttctt cttcttcgcc
     1921 ccgctgttca tcccgttctt cgtgatggcg atcccgatca tcgacacggc gttcgcgatc
     1981 atccgccggg cccgcaagcg cgccgggctg gccgaggccg acaaggacca cctgcaccac
     2041 cggctcatgc gcctcggcca cgggcagcgc cgcagcgtcg ccatcctctg gctctggtcg
     2101 gggctgctgt cgctgttcgt gctgtacccc gtgtacacgg gcaagggcaa cggcctggtg
     2161 cccatcgccg tagcggccct ggcgctcatc ctgctcacct tcttccaccc cgggacccgc
     2221 cgggcgcgcc agcaggaggc ggccgaggag gagcaccgcc ggctccagca gatcgccgag
     2281 gccgacaccg agccggtggg cgacgacgcc gtcgaggtgc ccgccgacga cctcgatccc
     2341 gtcgacgcca ccgccgatcc cggcgcctcc tgaccaggcc gatcgccggc gcgctcgggt
     2401 gcgggggccc gggacctgca catcggctgc agatttgccg gattgcaggg atccgcctac
     2461 attgagattc gattcacaag cacctcccga gcgcacctgc gagactgcag cgccggggcg
     2521 gcttggtccc acacctccat gagcgactcc acgtcacgca tcacgggcaa cgaagccccc
     2581 gcaccatcgc ccgccgtcgg ggtgcctgcg tcatcggcgc cgcgcgcctc ggccgatccg
     2641 ttcagccgca cgtcgtacgc caagtcgctc aaccgcgggt acggcgacgc catgggccgc
     2701 ggcctcgagc tggccatgac cctcgtgttc atggtggcga tcggccgcct cgccgacttc
     2761 gtgttcggta cctaccccat ctgcacgatc gtgttctcga tcctcggctt cgccgggatc
     2821 accgtgaagc tcttcttcgg gtacgacctc gagatgcgca agcacgagga gaacgccatc
     2881 tggaaccgca agagcgagtc cgcctgatgg ccaccacggt gcgcgccaag ccctcggtga
     2941 tcgagagccg cgacctcggc cccgcgcccg aggcccaggt ggcacgcgac ctcatcaagc
     3001 gggggctcat cgccctcccc ttcgccctgg tgctcggtgc catcggctgg ggcaccgccg
     3061 gcatcgcatc cgtggcctac gccgccgggc tcatcctcgt gaacttcgcc gtggccgccg
     3121 ccctgctcgg ctgggccgcc cgcgtctccc tcggtctgct gatgggcgtc tgcctgttcg
     3181 gcttcttgat ccgcatggcc gccatcaccg cagcggtgct gctggtgcgc gaccagtcct
     3241 gggtggcccc cgtgccgctc gccgtcacgt tgatcgtgac ccacctcggc ctcctgttct
     3301 gggagaccaa gttcgtatcc gcatccctgg cctttccggg cctgaagccg tcttctgagg
     3361 agcaatgagc gtgctcgcac tcgaattccc gccgctgagc cacatcacca actggggtgg
     3421 ctacgtcggc gatggcgaaa tctgggagat caacaagatc gtcctggtgt acggcttcgc
     3481 gctgctgttc acctggctgc tcttcttcct cggccgcaag ggcgagatcg tgccgaccgg
     3541 tgcccagaac ctcgccgaga tgtcgatcga gttcgtcgag gacggcatcg tgatggacac
     3601 gatgggcgag gaggggaaga agtacacccc cttcctcctc agcctgttcc tcttcatctt
     3661 cttcacgaac atcttcgagg tggtgccggt catccagatg ccggcctcgg ctcgcatcgc
     3721 catgccgatg accctcgcga tcatcgccta cctcgtgtac cacggcgccg gcttcaagag
     3781 ccacggcctc ggctacttca agcacgccct cgtgccgccg ggcgtgccga tcggcatcct
     3841 cccgctcgtg gtcatcatcg agttcgtctc gaagttcctg gtccagccct tcagccacac
     3901 ggtgcgtctg ttcgccaact tgctggccgg ccacatcctg ctcgtcacct tcgccgtgct
     3961 cacgagcgcg ctgatcaccc ccaagtggta cgcgatcttc gccccgctcc ccttcgctgg
     4021 cgttgtcttc ttcaccgcct tcgaggtgtt cgtgtccttc ctccaggcgt acgtgttcac
     4081 gctgctcacc ggcgtgtaca tcaatgcggc tgcgagccac gagcactgat cccgatcacc
     4141 tccatccaac caagcaccac gcaacccaga aaggctccga aatgagcatc gctgtcctgg
     4201 ccgccgaagg cctgaccaac aagggcgtcg ccgccctcac ctatggcctc ggcgccatcg
     4261 gccccggcat cggcatcggc tacctggtcg gccagtccgt ccaggccatg gcccgtcagc
     4321 ccgagtccgc cggcatggtg cgcaccacca tgttcctggg catcgcgttc gtcgaggcgc
     4381 tcgcgctcat cggcttcgtg gtgttcttcc tcgccggcgc ctgatcggga gacgaccaca
     4441 tgaagcgaac ccgactgctg ctggttgcag cagccatcgt ggcggtgggg ctcttcggct
     4501 tcgcccgtcc gtcgatggca caagaagcgt ctggcgagtc gacgtctgag gtcagccact
     4561 cggtgcacga gtgcatcgag ctgatggagg acgcgaactc gatcgacgtc gagtgccaga
     4621 aggcaccgaa cccgctcgtt cccgagaaga acgaggtcat ctggggcttc gtcggtttca
     4681 tcttggtctt cggcttcctc tactggaagg ccgtcccctc gatgaagaag ggcatgaacg
     4741 cccggacgga gcgcatcaag ggcgacctcg atgccgccga ggcgcagcgc gccgaggcct
     4801 ccgagatcct cgcccagtac aaggcgcagc tggctgacgc ccgcacggag tcggcccgca
     4861 tcatcgagga ggcccgccag gccgccgacg aggtgcgtgc caacctccag acgaaggcca
     4921 cggccgacat cgccgagctg cgtgctcggg ccaccgccga catcgagtcg gccaagcagc
     4981 aggccgtcgc cgacctgcgc ggcgaggtca cgtcgctggc catcggcgcc gccgagcagg
     5041 tggtcggccg caacctcgat gccgagacca acgccgccct cgtggaggcg tacatcgacc
     5101 aggtgggagc gaacagctga tggccgacga ccgcaccacg gcgtacgccg aggcactctt
     5161 cgcggtcgcg cggaccgaag gccccctctc cgaggtcgaa gacgagctgt tccgggtcgc
     5221 ccaggtggtg agggggaacg acgagctgcg tgacaagctc gccgaccccc acatcccggt
     5281 ggcgacccgc cagcagatca tcatcgacct cctctcgggc aaggccgagc cgaccaccgt
     5341 gagcctcgtc tcgctggtgg tcgccaacgg ccgcatccgg gagctcccgg ccatcgtgga
     5401 cgagctcctc gccatggcgg ccgcggaagg caacaaggag gtggccgaga tccgctccgc
     5461 catcgccctc accgacgacc agaagtcccg cctcgccgag gccctcggca aggccacggg
     5521 caagcaggtc gaggtccggg tcatcatcga cccgtccatc aagggcggca tcgtggccca
     5581 ggtcggagac accgtcatcg acggctccgt ccgccgccgc ctcgaacagc tcaagaacgc
     5641 cctctaggca cccgcccaga ccactcgcca cccggcacag gagaaacggc acatggccga
     5701 gctcacgatc aacatcgaag acatcaccgc tgcgcttcgc aagaacctcg agggcttcac
     5761 gcccgacgtg tccgccgccc aggtcggccg cgtgctcgag gtcggcg
//
LOCUS       DATMFS010000519         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_63594, whole genome shotgun sequence.
ACCESSION   DATMFS010000519 DATMFS010000000
VERSION     DATMFS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63594"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VNQ33_09815"
     CDS             complement(<1..1268)
                     /locus_tag="VNQ33_09815"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWJ98444.1"
                     /translation="MADTDPDDAPDTPLTGSALRRGARLASLPAGFAARTTWGIGRRL
                     VGAPAAAVMTDVQRRTADQVFSVLGQLKGGAMKFGQALSIFEAALPEEVIAPYREALT
                     KLQDAAPPMGTPTVRRVMEREFGPDWPERFPDFEMTPAAAASIGQVHRSGFVAEPGAE
                     PVEVAVKLQYPGAAEALNSDLRQIGRLARLLGTMMPGVDVKALVRELQERVAEELDYS
                     LEADAQARFAEAFADDPNIVVPRPLAHTERALVTTWLDGDRSLADVITDGTPEERDRL
                     GETYVRFLFEGPARAGLLHADPHPGNFRVLPDGRLGVVDYGAVARLPDGLPAPMGPLL
                     RAAADGDYVTVAEGLRREGFVRQGSNVDPEVLGRYLGPIVEPVAVEAFTFDRDWLRRQ
                     GQRLSSPGAEGMGTALKLTVPPSYLLIHRV"
     gene            complement(1305..>2076)
                     /locus_tag="VNQ33_09820"
     CDS             complement(1305..>2076)
                     /locus_tag="VNQ33_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98445.1"
                     /translation="GPRLLPGALLLRRDARTLQVGTSPGVPVRDRPGLARVLRLLDGS
                     LPLPVLAELVARDVPEFADDLHAVLRELGAAGAVLDPVSAPPSPRVAVRHDRSTPDFA
                     ALLIDALGPVPAEPDLEILVSAGEPGRSTVEHLVGAGIAHLPVVLDERRVRIGPLVVP
                     GATPCLGCHDAHRAAADPGWRALIPQFERGRLLACGLAPGVLLRAAAEVLHQVHCLRN
                     GARPPALGRVVSVTSPHDVPEVEDVPFATDCACRLLAA"
BASE COUNT          278 a          739 c          819 g          240 t
ORIGIN      
        1 cacacgcggt ggatcagcag gtagctcgga ggcacggtga gcttcagcgc ggtgcccatg
       61 ccctccgcac ccggcgacga caggcgctgg ccctgacggc gcagccagtc gcggtcgaag
      121 gtgaacgcct cgaccgccac gggctccacg atcgggccca ggtagcgccc cagcacctcg
      181 ggatcgacgt tgctgccctg gcgcacgaat ccctcgcggc gcagcccctc ggccaccgtg
      241 acgtagtcgc cgtcggccgc ggcccgcagc aacgggccca tcggcgcggg cagcccgtcg
      301 ggcagccgcg ccacggcgcc gtagtccacc acgccgagtc gcccgtcggg cagtacgcgg
      361 aagttgcccg ggtgcgggtc ggcgtgcagc aggccggcgc gcgccggtcc ctcgaacagg
      421 aagcgcacgt aggtctcgcc cagtcggtcg cgctcctcgg gcgtgccgtc ggtgatcacg
      481 tcggccagcg agcggtcgcc gtccagccac gtggtcacga gggcgcgctc ggtgtgcgcg
      541 agcggtcgcg gcacgacgat gttcgggtcg tcggcgaacg cctcggcgaa ccgggcctgc
      601 gcgtcggcct cgagcgagta gtcgagctcc tcggcgaccc gctcctgcag ctcgcgcacg
      661 agggccttca cgtcgacgcc cggcatcatc gtgccgagca ggcgggccag ccggccgatc
      721 tggcgcaggt cggagttcag cgcctcggcg gcgccggggt actgcagctt gacggcgacc
      781 tcgacgggct cggcgcccgg ctcggcgacg aagccgctgc ggtggacctg gccgatcgag
      841 gcggccgcgg ccggcgtcat ctcgaagtcg gggaagcgct cgggccagtc gggtccgaac
      901 tcgcgctcca tgacgcgcct caccgtgggc gtgcccatgg gcggcgccgc gtcctgcagc
      961 ttcgtcagcg cctcgcggta cggcgcgatg acctcctcgg ggagcgcggc ctcgaagatg
     1021 ctcagcgcct ggccgaactt catcgcgccg cccttgagct ggccgaggac ggagaacacc
     1081 tggtcggcgg tgcgtcgctg gacgtcggtc atgaccgcgg cggcgggggc gccgacgagt
     1141 ctgcggccga tgccccaggt cgtgcgggcc gcgaagccgg cgggaaggct ggccaggcgg
     1201 gccccgcgtc gcagggcact gccggtcagg ggagtgtcgg gagcgtcgtc cgggtcggtg
     1261 tcggccacgt ggtcaggata gtggcgcctg ccgcatccga ccggtcacgc ggcgagcagc
     1321 cggcaggcgc agtcggtggc gaacgggacg tcctcgacct cgggcacgtc gtgcggggag
     1381 gtcaccgaca cgacccggcc gagtgccggc gggcgggctc cgttccgcag gcagtggacc
     1441 tggtgcagta cctcggcggc ggcgcggagc agcacgcccg gtgccagtcc gcacgccagt
     1501 aggcgacccc tctcgaactg cgggatgagg gcccgccacc ccgggtccgc cgcggcgcgg
     1561 tgcgcgtcgt ggcatcccaa gcatggcgtg gcaccgggca cgacgagcgg cccgatccgc
     1621 acgcgccgct cgtcgaggac gacgggcagg tgcgcgatcc cggcaccgac gaggtgctcc
     1681 acggtggagc gaccgggctc gcccgcgctc acgaggatct cgaggtcggg ctcggcgggc
     1741 accggtccca gcgcgtcgat cagcagggcc gcgaagtcgg gagtggagcg gtcgtggcgc
     1801 acggccaccc ggggactggg aggtgccgag acgggatcga gaacggcacc cgcggcaccg
     1861 agctcgcgca ggacggcatg gaggtcgtcg gcgaactcgg gcacgtcgcg cgccacgagc
     1921 tcggccagga cgggcagggg cagcgatccg tcgaggaggc gcagcacgcg cgccaagccc
     1981 ggccggtcgc gcaccgggac gccggggctg gtgccgacct ggagcgtgcg ggcatcgcgc
     2041 cgcaggagca gggcacccgg caggaggcgg ggaccg
//
LOCUS       DATMFS010000520         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_297919, whole genome shotgun sequence.
ACCESSION   DATMFS010000520 DATMFS010000000
VERSION     DATMFS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297919"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VNQ33_09825"
     CDS             <1..1255
                     /locus_tag="VNQ33_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ98446.1"
                     /translation="RAWVDGERTASSLTALDTGPRGPIGVYQVVDNERKLSKAEIEAG
                     STYGSPKDGRARLEPALAGGCGARFSSLEDEPRRLSGPVAVAASPASDLVWVVGEFSH
                     SVSVVRCDGGPESSRSTTVAAFDVGEGARGIVLDADGRTAYVDVGFDHQIAELDLPVD
                     AASVQAGDAIERTEPVAVGRRKVEDQYLTPLAQEGRRMFNDATDTHLTPFGVVTCGSC
                     HPSAGEDGLSWRIESADAATRSIERKVRRTPPAWQVDTEVKPLHWNGEFTSSDDLALS
                     TIQQLLGGDGLLVDTAAISAYMAEVSAPPARPTGGVDQTAKARQEGDALLTELGCTSC
                     HAGDAGTDGKPHHVLSPSTVADGDLPAVVTPPLRAVRGRAPYGHDGRAPDLASLLRAH
                     GDGEGGSIELTPEQLAALVAYLDTR"
     gene            complement(1287..1754)
                     /locus_tag="VNQ33_09830"
     CDS             complement(1287..1754)
                     /locus_tag="VNQ33_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWJ98447.1"
                     /translation="MPSSSAGLLLHRTGLDGLEVLLVHPGGPFWANKDEGAWSIPKGE
                     HDDGDDPHACALREFEEELGSPPPPADEPDRDLGEVRLKSGKRVRAFTRAGTLDVDEI
                     TSNTFEVEWPPRSGRRQTFPEVDRAGWFPVDEARRKLNPAQAELVDRLLTALA"
     gene            complement(1757..>2087)
                     /locus_tag="VNQ33_09835"
     CDS             complement(1757..>2087)
                     /locus_tag="VNQ33_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98448.1"
                     /translation="HTVDIAVAFGPPPPPTDRLWHTARLTWRTVPYALGQEGISAAGP
                     VVFSLDAPDRSTWTFGEPADAATTITGSALDLCTVAGQRGSAAGTSLHGEGPDADDVL
                     RLMRTFA"
BASE COUNT          275 a          739 c          804 g          269 t
ORIGIN      
        1 ccgggcctgg gtggacggcg agcggacggc atcgtcgttg acggcgctcg acaccgggcc
       61 ccgtggcccg atcggcgtgt accaggtggt ggacaacgag cggaagctgt cgaaggcgga
      121 gatcgaggcc gggtccacct acgggagccc gaaggacggc cgggctcgtc tcgagccggc
      181 gctcgccggg gggtgcgggg cccggttctc gtcgctcgag gacgagcctc ggcggctgag
      241 cgggcccgtc gccgtcgccg cgtcccccgc cagcgacctg gtctgggtcg tcggcgagtt
      301 cagccactcg gtgtcggtcg tccgctgcga cggcggaccc gagtcgtcgc ggtcgacgac
      361 cgtcgccgcg ttcgacgtgg gagagggagc gcgggggatc gtgctcgacg ccgacggccg
      421 gaccgcctac gtcgacgtcg ggttcgacca ccagatcgcc gagctcgacc tgcccgtcga
      481 cgccgcttcg gtgcaggccg gcgatgccat cgagcggacc gagccggtgg ccgtcgggcg
      541 gcggaaggtc gaggaccagt acttgacgcc gctcgcccag gagggccggc gcatgttcaa
      601 cgatgccacc gacacccacc tgaccccgtt cggcgtggtg acgtgcggtt cgtgccaccc
      661 gtccgcgggc gaagacgggc tcagctggcg gatcgagagc gccgatgccg ccacgcgcag
      721 catcgagcgc aaggtccgcc gcaccccgcc ggcctggcag gtcgacaccg aggtgaaacc
      781 gctccactgg aacggcgagt tcacgagcag cgacgacctc gccctctcga cgatccagca
      841 gctgctcggt ggcgacggcc tgctcgtgga cacggcggcg atcagcgcct acatggccga
      901 ggtgagcgcg ccgccagcgc gtccgaccgg tggcgtcgac cagacggcga aggcccggca
      961 ggagggtgat gccctgctga ccgagctggg ctgcacgtcg tgccacgccg gcgacgccgg
     1021 gaccgacggg aagccgcacc acgtgctgtc cccctcgacg gtggccgacg gcgacctgcc
     1081 ggcggtcgtc acgcccccgc tgcgcgccgt gcggggtcgg gcgccctacg gccacgacgg
     1141 gcgcgcgccc gacctggcgt ccctgctccg ggcccacggc gacggcgagg gcggctcgat
     1201 cgagctcact ccggagcagc tggcggcgct ggtcgcctac ctcgacaccc gctgacgcgc
     1261 ggcgggctca gcccggctca gccggatcag gcgagggcgg tgaggaggcg gtcgaccagc
     1321 tcggcctggg cggggttgag cttgcggcgg gcctcgtcga ccgggaacca gcccgcccga
     1381 tcgacctcgg ggaacgtctg acgccgcccg gaccgaggcg gccactccac ctcgaaggtg
     1441 ttgctcgtga tctcgtcgac gtcgagcgtg ccggcgcggg tgaacgcccg gacccgcttg
     1501 ccgctcttca accgcacctc gccgaggtcc cggtcgggct cgtcggccgg gggtgggggc
     1561 gagcccagct cctcttcgaa ctcccgcagc gcgcacgcgt gcgggtcgtc accgtcatcg
     1621 tgctcgccct tcgggatcga ccaggcacct tcgtccttgt tcgcccagaa cgggccgccg
     1681 gggtgcacga gcaggacctc gaggccgtcg agcccggtgc ggtggagcag gagcccggcg
     1741 ctagacgatg gcatcgtcag gcgaacgtgc gcatgaggcg cagcacgtcg tcggcgtcgg
     1801 gtccctcgcc gtggagcgag gtgcccgccg ccgatccccg ctggcccgcg accgtgcaga
     1861 ggtcgagtgc cgagccggtg atggtggtgg cggcatcggc gggttcgccg aaggtccagg
     1921 tggatcggtc gggtgcgtcg agcgagaaca cgacggggcc agctgccgag atcccctcct
     1981 ggccgagcgc gtagggcacc gtccgccagg tgagccgggc cgtgtgccag agccggtcgg
     2041 tgggcggggg cggcggcccg aacgccacgg cgatgtcgac cgtgtgg
//
LOCUS       DATMFS010000521         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_63692, whole genome shotgun sequence.
ACCESSION   DATMFS010000521 DATMFS010000000
VERSION     DATMFS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63692"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VNQ33_09840"
     CDS             complement(<1..988)
                     /locus_tag="VNQ33_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98449.1"
                     /translation="MVALRRPASAAAVAALAATVVLSSCTRTSGSAHDRPDPHTWASR
                     TATVLTHGLQATPDGPIPQVATPIAGTAPLFDRAGAELALAAVDDLGRPSSKVDDELA
                     DAIGPDGTVDDGLSPTEVLTWYVARLAAAPGDPAPSVRRRVEEVADALEDTVASHLTA
                     SSDDLFTQVFWVRAAAARQSSPTIDVAGFDPAAACESSVPAPTDEIEAALAVAGVASE
                     IATAAGTRCSRAVVEGWQTLQAPAEAWLRGAPRDEELDATGVGALSAIARLGRAGAFD
                     DDLATAAGDRLASSLTGDEVETTPAVVLEAVDALEAAGVRRSGLGPETAATLR"
     gene            complement(989..1567)
                     /locus_tag="VNQ33_09845"
     CDS             complement(989..1567)
                     /locus_tag="VNQ33_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98450.1"
                     /translation="MSTKTAARAVLGLAALTTGVSAVAPAPADAGSYTQTDETLSYYG
                     SGATRGHVRTYVRAYSRDFGCGLQDCASIRDMYGSVNGYKDGNNASLPDAVKMRLVAG
                     FGGVSLSVSLGVSAGGPSGGVAFRDLGASCGTADVQNSGAEISINAPGEICKASTYGW
                     VSNIDVTTGGLYRRGTAWTTVTAFDRVSVGGF"
     gene            1827..2195
                     /locus_tag="VNQ33_09850"
     CDS             1827..2195
                     /locus_tag="VNQ33_09850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWJ98451.1"
                     /translation="MSEKPEITIPNEAPPVELVLEDLVVGDGPEAGPGPVEVHYVGVA
                     WSTGEQFDASWDRGDTFGFRLGAGQVISGWDQGVAGMKLGGRRKITIPPHLGYGERGA
                     GGVIKGGETLVFVVDLLGVG"
BASE COUNT          373 a          809 c          915 g          336 t
ORIGIN      
        1 cacgaagggt cgcggcggtc tccggcccga gccccgagcg ccgcacgccc gcggcctcga
       61 gagcatccac cgcctcgagc acgacggccg gtgtggtctc gacctcgtca ccggtgaggc
      121 tgctggcgag gcggtcgcct gctgccgttg cgaggtcgtc gtcgaacgcg ccggctcgac
      181 cgaggcgggc gatggcgctc agggcgccga caccggtcgc gtcgagctcc tcgtcgcggg
      241 gcgcgccgcg caaccacgcc tcagcaggag cctgcagcgt ctgccacccc tcgacgaccg
      301 ctcgggagca gcgggtgccg gcggccgtgg cgatctccga tgcgacgccg gccacggcga
      361 gcgcggcctc gatctcatcg gtcggggcgg ggaccgagga ttcgcaggcc gcggcggggt
      421 cgaacccggc gacgtcgatg gtcggactgc tctggcgggc ggccgctgct cgaacccaga
      481 agacctgggt gaagaggtcg tcgctcgacg ccgtcaggtg ggaggcgacc gtgtcctcga
      541 gcgcgtcggc gacctcttcc actcggcggc gcaccgaggg agccgggtcg ccaggagcgg
      601 cggccaagcg ggcgacgtac caggtgagga cctcggtcgg ggagaggccg tcgtccacgg
      661 tgccgtccgg tccgatcgcg tcggccagtt cgtcgtcgac cttcgacgaa ggtcgaccga
      721 ggtcgtcaac ggcggccaga gcgagctccg caccagcacg gtcgaagagg ggcgcggtgc
      781 ccgcaatggg ggtggcgacc tgcgggatcg ggccgtcggg ggttgcctgc aacccgtggg
      841 tgagcacggt ggcggtgcgg gaggcccagg tgtgcgggtc cgggcgatcg tgcgcagagc
      901 cggaggtacg ggtgcagctg ctcagcacca cggtggcggc cagcgcggcg accgcagccg
      961 cgctcgccgg ccgccgaagg gccaccacct agaagccgcc gaccgagacg cgatcgaacg
     1021 cggtcaccgt cgtccacgcc gtgccgcggc ggtagaggcc gccggtggtg acgtcgatgt
     1081 tgctgaccca gccgtaggtg ctcgccttgc agatctcgcc tggggcgttg atgctgatct
     1141 ctgcaccgga gttctggacg tcggcggtgc cgcaggaagc gcccaggtcg cggaacgcga
     1201 cgcctccgct cggacctccg gcactgactc cgaggctcac gctgaggctc accccgccga
     1261 agccggcgac caggcgcatc ttgaccgcgt cgggcagcga ggcgttgttc ccgtccttgt
     1321 agccgttgac ggagccgtac atgtcccgga tactggcgca gtcctggagg ccgcagccga
     1381 agtcgcggga gtaggcccgg acgtacgtcc gcacatgacc ccgggtcgcc ccgctgccgt
     1441 aataggacaa cgtttcgtcg gtctgggtgt aggagcccgc gtcggcgggg gcgggcgcga
     1501 cagcgctgac cccagtggtg agagcggcga ggccgagcac agctcgggcg gcggtcttgg
     1561 tggacatcag cggtctccga tgcagacgat acgtatagag aagatgtaga cgaaaaggcc
     1621 tggaatcgtc aattcggaca tcaagagggg ctgaaccacc aaatcggaca tgaagcgtgc
     1681 tggcgagcga gatcggacat gacaccacgg gaagcccatc accgaacacc gaaaccgctg
     1741 cggacgcacc gggccccagc ccagcggcgg aggccgtgga ccggtcgggg tcaggcgcgg
     1801 cgggctggcc gatagggtcg gcggccatga gcgagaagcc tgagatcacc atcccgaacg
     1861 aagcgccgcc cgtcgagctg gtgctggagg acctcgtcgt cggtgacggc cccgaggccg
     1921 ggcccggccc cgtcgaggtc cactacgtgg gggtggcgtg gtccaccggc gagcagttcg
     1981 acgcctcctg ggaccgcggc gatacgttcg ggttccgcct gggcgccggc caggtcatct
     2041 ccggctggga ccagggcgtc gccggcatga agctcggcgg ccgccgcaag atcacgatcc
     2101 cgccgcacct gggctacggc gagcgcggcg ctggcggcgt catcaagggc ggcgagaccc
     2161 tcgtcttcgt cgtcgacctc ctcggcgtcg gctgaaggat ttcgggtcat ctgagcaccg
     2221 cctcggtgct cagatgaccc gaaatcgcac tcggcggcgg aacgctttgg gatggtcggg
     2281 gaccgggccg gtaccgtcca cggcgtgctg aagctggttc tccccaaggg ttcgttggaa
     2341 aaggccacgt tggagctgtt cgcggccgcg gacctgcagg tgaaccggtc ctcgaccgtc
     2401 gactacaagg cgatgatcga cgacccgcgc atc
//
LOCUS       DATMFS010000522         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_297969, whole genome shotgun sequence.
ACCESSION   DATMFS010000522 DATMFS010000000
VERSION     DATMFS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297969"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNQ33_09855"
     CDS             complement(<1..407)
                     /locus_tag="VNQ33_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98452.1"
                     /translation="MTTTERRATTQVRSRHRSDAGAEATSAAEAVAGLIGVWLGDPPP
                     VRFEFWDGSSLGPGADEAAGVVEVRSPDAVRRILWAPGELGVGRAYVAGDLGLRGDLY
                     DTLSALRDGYRPSASATAAAVPAGVRAAHRLGV"
     gene            693..1139
                     /locus_tag="VNQ33_09860"
     CDS             693..1139
                     /locus_tag="VNQ33_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWJ98453.1"
                     /translation="MTEGRYLIGKAAVMAGVTNRTLRYYQELGLIDPATSPAGTRIYS
                     DRDVERLKRIIQLRDVVGLDLERIAELLRAEDRLEELRTEVRHGTSPKRRQEIIQEAI
                     AVNAHMLDVVAAKLEALQAYLDELTADATRYRAVLDRERTRDTSTP"
     gene            complement(1475..4021)
                     /locus_tag="VNQ33_09865"
     CDS             complement(1475..4021)
                     /locus_tag="VNQ33_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HWJ98454.1"
                     /translation="MTATTTITIPDLALVVLIGPSGAGKSSFAARHFASSEVLSSDFC
                     RLLVSDDENDQAATPAAFDVLGYIAGKRLEAGRLTVIDATSVQCDARAPLVSLAREHH
                     VLPVAIVLDVPEKVCAERNAARPDRNLPAHAVRRQHQQLRKSIKGLQREGFRRVFVLK
                     GTDEIDAVSIERERRWTDRRNDHGPFDFIGDVHGCADELVALLTELGYDIAPDRSTAS
                     HQAGRRAFFVGDLVDRGPDSPGVLRLAMGMVRDGTAACVPGNHENKLWRALNGRNVQV
                     SHGLAETLAQLEGEPDEFRAEVATFIDSLISHAVLDDGRCVVAHAGLPERLHNRSSGA
                     VRSFALYGDTTGETDEFGLPVRYPWANDYRGSAVVVYGHTPVPEAEWINNTICIDTGC
                     VFGGKLTALRYPERELVSVPAARTYWEPTKPLRPETNEPERPAELLDATDVLGKRIID
                     TRLQPRITIGAENAAAAFEVMSRFAIDPRWLIYLPPTMAPTATSAHPDLLEHPTEAFA
                     AFRRDGVAQVICEEKHMGSRAVVIVCRDAEAAARRFKIDDAAGGTIYTRTGRAFFGDP
                     TWQARVIERTRRVVDAADLWDLLDTDWVALDAELLPWSAKAEDLLRTQYAAVGAAGHA
                     MTTRASELLAAARTRGIHVDELAQRTGERVVAIDRFADAYRRYCWSVEEPDDLQLAPF
                     VVLAAEGTLLAERDHAWHLSLAERLVAAGDGFIRPTRNLTVDLDDSRSEAAAVTWWEG
                     LVGNGGEGMVVKPLESIVTGARGLIQPGIKCRGPEYLRIIYGPDYLAPHNLERLRNRG
                     LGMKRSLAQREFALGIEALERFVAGEPLHRVHECVFGVLAMESEPVDPRL"
BASE COUNT          606 a         1341 c         1528 g          632 t
ORIGIN      
        1 atcacgccga ggcgatgggc ggcgcggacc ccggccggca cggccgctgc ggtggccgac
       61 gcgctggggc ggtagccgtc gcgcagagcc gagagcgtgt cgtagaggtc cccgcgcagg
      121 ccgaggtcac cggccacgta ggcccggccg acgccgagct cgcccggggc ccacaagatg
      181 cgtcgaaccg catccgggga gcggacctcg acgacacccg ccgcttcgtc ggcccctggt
      241 ccgagcgagc tgccgtccca gaactcgaac cgcacggggg gtgggtcgcc gagccacacc
      301 ccgatgagcc cggccaccgc ctcggcggcc gaggtggctt ccgcccccgc gtcgctgcga
      361 tggcgcgatc ggacctgggt ggtcgctcga cgttcggtgg tcgtcatcgt ctctcctccg
      421 gcgcagttca ggggttgggg tggtggcagc tcgccgccga tcgtggcgac gcctctgcga
      481 tacgtcagag cgattcggtc atgctcgtcc ggcctggctg ggtcttggtc ctggccggga
      541 ccgccagcca gggatcatcg atctcacgtt aaggtgagat cacgggactt ccaccctcgg
      601 gacacgagcg agcgctggcg ccgcgacatc ctcctggcgg ccagccgcac tgcccgccgg
      661 gcgcccggca ccggacctgg aagagtggct gcatgaccga aggccgctac ctgatcggca
      721 aggccgccgt gatggccggc gtcaccaacc gcacgctccg ctactaccaa gagctgggcc
      781 tgatcgaccc ggccacgagc cccgccggca cccgcatcta ctccgatcgc gacgtcgagc
      841 gtctcaagcg gatcatccag ttgcgcgatg tggtgggcct cgacctcgaa cgcatcgccg
      901 agctgctccg agcggaggac cggctcgagg agctccggac cgaggtccgc cacggcacca
      961 gccccaagcg ccgccaagag atcatccagg aggccatcgc ggtcaacgcc cacatgctcg
     1021 acgtcgtcgc cgccaagctg gaagcgctcc aggcctacct ggacgagctg accgcggacg
     1081 ccacccgcta ccgagccgtg ctcgaccgcg aacggacccg cgacacgtcg acgccgtgac
     1141 cctcatgggg ccaagaagct cggtggtttc gcctgccgct ccgcgggtcc ggcatcgttc
     1201 tggagccttg ccagcccagc gaggatcggg gcccgccacc tacgctcggc cgccaggaag
     1261 accgaatcga gccgacggtg atccggtggt gcgtgcccac cttgcggacg tcgagcttgc
     1321 cgccgtccat cagcttgcgg accgggcccg ggacaggccg agcacctgcc cggcctgggt
     1381 cggggtgacc tcgctgagac ccttcgtgag cgccgtgtcg tagccgctga ccttggcatc
     1441 gacgatgcgg tgcccggccg tagacgaaac gggttcagag tcgggggtcg accggttcgg
     1501 actccatggc gagtacgccg aagacgcatt cgtggactcg gtggaggggc tcgccggcga
     1561 cgaagcgttc gagagcttcg atgccgaggg cgaactcacg ctgggcgagg gatcgcttca
     1621 tgccgaggcc gcggtttcgg agtcgttcga gattgtgggg agcgaggtag tcggggccgt
     1681 agatgatccg caggtactcc gggcctcggc acttgatgcc gggctggatg agaccgcgcg
     1741 cgccggtcac gatcgactcg aggggtttga cgaccatgcc ttcgccgccg ttcccgacga
     1801 gcccttccca ccaagtgacg gcagcagctt cgctccggga gtcgtcgagg tcgacggtga
     1861 ggttccgagt cgggcggatg aacccatcac cggcggcgac gagccgctcg gcaagagaca
     1921 ggtgccaggc gtgatcacgc tccgcgagga gcgtcccttc ggcggccagg acgacgaagg
     1981 gggcgagctg gaggtcgtcg ggctcctcga cggaccagca gtaccggcgg taggcatcgg
     2041 caaaccggtc gatggcgacg acccgctcgc cggtgcgctg ggcgagctcg tcgacgtgga
     2101 ttccacgcgt gcgggctgcg gcgagcagtt cgctggcgcg tgtggtcatg gcgtggccgg
     2161 cggcgccgac cgccgcgtac tgggttcgca gcaggtcttc cgccttggcg ctccacggaa
     2221 gcagctcggc gtcgagggcg acccagtcgg tgtcgagcag atcccagagg tctgcggcat
     2281 cgacaacgcg tcgggtgcgt tcgatgaccc tggcctgcca tgtcggatcg ccgaagaacg
     2341 cccggccggt gcgggtgtag atcgtccctc cggctgcgtc gtcgatcttg aaccggcgag
     2401 cagcggcctc cgcatcacgg cacacgatga cgaccgcccg ggagcccatg tgcttctcct
     2461 cgcagatcac ctgggcgacg ccgtcgcgcc ggaaggcagc gaacgcctcg gtcgggtgtt
     2521 cgaggaggtc gggatgtgct gaggtcgcag tcggcgccat ggtcggcggg aggtagatga
     2581 gccagcgagg gtcgatggcg aagcggctca tcacctcgaa cgcggctgcg gcgttctccg
     2641 cgccgatggt gatgcgcggc tggagtcgcg tgtcgatgat gcgcttgccc aacacgtcgg
     2701 tggcgtcgag cagctcggct ggccgttccg gctcgttcgt ctcggggcgg agaggcttgg
     2761 tgggttccca gtaggtgcgg gccgccggga ccgagacgag ctcccgttcc gggtaccgca
     2821 gcgcggtgag cttgccgccg aacacgcagc cggtgtcgat gcagatggtg ttgttgatcc
     2881 actcggcttc ggggaccggg gtgtggccgt agacgacgac ggcagagccg cggtagtcgt
     2941 tggcccacgg gtagcgcacg gggaggccga actcgtcggt ctcgccggtg gtgtcgccgt
     3001 agagggcgaa gctgcgcacg gctccggagg agcggttgtg gagtcgttcg gggagcccgg
     3061 cgtgggcgac gacgcagcgg ccgtcgtcga gcacggcgtg gctgatgagg ctgtcgatga
     3121 acgtggcgac ctcggcgcgg aactcgtcgg gttctccttc gagttgggcc agcgtctcgg
     3181 cgaggccgtg ggagacctgg acgttgcggc cgttcagggc gcgccacagc ttgttctcgt
     3241 ggttgccggg gacgcacgcc gcggtcccgt cacgcaccat gcccatggcg aggcggagca
     3301 caccggggct gtcggggcca cggtcgacga ggtcaccgac gaagaacgcc ctccgcccgg
     3361 cctggtggct ggcggtcgac cggtcgggag cgatgtcgta gccgagctcg gtgagcaagg
     3421 cgacgagctc gtcggcgcag ccgtggacgt cgccgatgaa gtcgaacggg ccgtggtcgt
     3481 tgcggcgatc ggtccaccgt cgttcacgct cgatcgagac cgcatcgatc tcgtcggtgc
     3541 ccttcaggac gaacacgcgg cgaaagccct cgcgctggag acccttgatc gacttgcgga
     3601 gctgctggtg ctggcggcgc accgcgtgcg cgggcaggtt gcggtcgggt cgggcggcgt
     3661 tgcgttcggc gcacaccttc tccggcacgt cgagcacgat ggccacggga agcacgtggt
     3721 gctcgcgggc gaggctgacc agcggcgctc gagcgtcgca ctgcacgctg gtggcgtcga
     3781 tgacggtgag ccggccggct tcgaggcgct tgccggcgat gtagccgagc acgtcgaacg
     3841 cggcaggggt ggcggcctgg tcgttctcgt catccgatac aaggagccgg cagaaatctg
     3901 acgagagcac ctcactggac gcgaagtgcc gggcggcgaa gctggacttg cctgcaccgg
     3961 aggggccgat cagcacgacc agtgccaggt cgggaatggt gatcgtcgtg gtggcggtca
     4021 tcgggtgaac accgccatct gtgtcggcgg tcccacttgg ggatcgtccg ggccgatcgg
     4081 ggtgaacgcc acctggtagc cgtgtgt
//
LOCUS       DATMFS010000523         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_271608, whole genome shotgun sequence.
ACCESSION   DATMFS010000523 DATMFS010000000
VERSION     DATMFS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271608"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..598
                     /gene="rpsL"
                     /locus_tag="VNQ33_09870"
     CDS             227..598
                     /gene="rpsL"
                     /locus_tag="VNQ33_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWJ98455.1"
                     /translation="MPTIQQLVRKGRQDKPTKGKTPALKGAPQRRGVCTRVYTNTPKK
                     PNSALRKVARVRLSSGVEVTAYIPGEGHNLQEHSIVMVRGGRVKDLPGVRYKIIRGTL
                     DTSGVKDRKQARSRYGAKKEG"
     gene            600..1073
                     /gene="rpsG"
                     /locus_tag="VNQ33_09875"
     CDS             600..1073
                     /gene="rpsG"
                     /locus_tag="VNQ33_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWJ98456.1"
                     /translation="MPRKGPAPRRELMPDPIYRSVVVTQLVNKVLQRGKRSTAERIVY
                     SALEIVQSKTGTDDPVGTLKRAVDNVRPQLEVRSRRVGGATYQVPVEVKPRRANTLAI
                     RWLVGYSRQRREKTMAERLANEILDASNGIGASVKRREDLHKMAESNKAFAHYRW"
     gene            1195..>2002
                     /locus_tag="VNQ33_09880"
     CDS             1195..>2002
                     /locus_tag="VNQ33_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ98457.1"
                     /translation="MPEVVPIARYRNIGIMAHIDAGKTTTTERILYYTGKTYKIGEVH
                     EGGAVMDHMVQEQERGITITSAATTTFWRDHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDGVAGVEPQTETVWRQADKYGVPRMCFVNKLDRMGADFYKAVDSIKDRLGANVA
                     VLQLPIGYESEFTGVVDIVKMKAVIWSGEELGAKYDIVDIPDDLAEKAEQYRHELLDV
                     VGSADDSILEKYLGDEEITEAELLTAIRKATIANEIVPVLTGSSFKNKGVQ"
BASE COUNT          373 a          720 c          632 g          277 t
ORIGIN      
        1 agcacccggg ggcgtccagc aggagtgtcg gtcatcgcgt ctcgtccttc gccggcccgt
       61 cggtggtccg tcgggcaggc gccccggtgc ggccgaccga tcgtagcggc accgccgagc
      121 aggtcctttt gctcccagcc ggacgacgcc cctagcatgg caactcggct caatgacgga
      181 tgccgtcatc cgtgagccac cgccgaagtc atcgagaggt tcgttcgtgc ccaccatcca
      241 gcagctggtc cgcaagggcc gccaggacaa gcccaccaag ggcaagaccc cggcgctcaa
      301 gggcgccccg cagcgtcgtg gcgtctgcac ccgcgtgtac acgaacaccc ccaagaagcc
      361 gaactcggcc ctccggaagg tggcccgcgt ccgtctctcc agcggtgtcg aggtcacggc
      421 ctacatcccc ggcgagggcc acaacctcca ggagcactcc atcgtcatgg tccgtggcgg
      481 ccgtgtgaag gacctccccg gcgttcgcta caagatcatc cgcggcaccc tcgacacctc
      541 gggtgtgaag gaccgcaagc aggcccgcag ccgctacggc gcgaagaagg aaggctgaca
      601 tgccccgcaa gggtcccgcc ccccgccgcg agctcatgcc cgacccgatc taccggtcgg
      661 tcgtcgtcac ccagctcgtc aacaaggtcc tgcagcgcgg caagcgctcc accgccgagc
      721 gcatcgtcta ctcggccctg gagatcgtcc agtccaagac cgggaccgat gacccggtcg
      781 gtaccctgaa gcgtgcggtc gacaacgtcc gcccccagct cgaggtgcgc agccgccgcg
      841 tcggtggcgc cacctaccag gtccccgtcg aggtcaagcc ccgtcgggcc aacaccctcg
      901 ccatccgctg gctcgtgggc tactcccggc agcgccggga gaagaccatg gccgagcgcc
      961 tggccaacga gatcctcgac gccagcaacg gcatcggggc ctcggtgaag cgccgtgagg
     1021 atcttcacaa gatggcagag tcgaacaagg ccttcgccca ctaccgctgg tagcgcaaac
     1081 agaacctgag acccgggggt gcacgctccc gggttccgca ccgaccgggt ccgcctgctg
     1141 gcggttcccg gtcgtggcat gtcaccgcca acccacgtga actgagacag agagatgcca
     1201 gaagtcgtcc ccatcgcccg gtaccgcaac atcgggatca tggcccacat cgacgcgggc
     1261 aagaccacca ccacggagcg gatcctctac tacaccggca agacctacaa gatcggcgag
     1321 gtgcacgagg gcggcgcggt catggaccac atggtccaag agcaagagcg cggcatcacc
     1381 atcacctcgg cggccaccac cacgttctgg cgcgaccacc ggatcaacat catcgacacc
     1441 cccggccacg tggacttcac ggtcgaggtg gagcggtcgc tgcgcgtgct cgacggtgcg
     1501 gtcgccgtgt tcgacggcgt ggccggcgtg gagccccaga ccgagaccgt gtggcgccag
     1561 gccgacaagt acggcgtccc gcgcatgtgc ttcgtcaaca agctcgaccg gatgggcgcg
     1621 gacttctaca aggccgtcga ctccatcaag gaccggctcg gtgcgaacgt ggcggtgctc
     1681 cagctgccga tcggctacga gtccgagttc acgggcgtgg tggacatcgt caagatgaag
     1741 gccgtcatct ggtccggcga ggagctcggc gccaagtacg acatcgtcga catccccgac
     1801 gacctcgcgg agaaggcgga gcagtaccgc cacgagctgc tcgatgtcgt cggctcggcc
     1861 gatgactcga tcctcgagaa gtacctcggc gacgaggaga tcaccgaggc ggagctgctc
     1921 accgccatcc gcaaggccac catcgccaac gagatcgtgc cggtgctcac cgggtcgtcc
     1981 ttcaagaaca agggcgtgca gc
//
LOCUS       DATMFS010000524         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_298003, whole genome shotgun sequence.
ACCESSION   DATMFS010000524 DATMFS010000000
VERSION     DATMFS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298003"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VNQ33_09885"
     CDS             complement(<1..655)
                     /locus_tag="VNQ33_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98458.1"
                     /translation="MAIATRRVGLRGRLLTLVLVPTLVAGALAGHTAADKRRAADVAG
                     SVHSEVGGLLALVDIRTAMMSARIPVEVQIRSEALGLDPEAVLKLLDMDGQRFGDLAD
                     VAAKLRAMPERIRPFEPEDLDTLRDEIRHGADVEVIDRFTTLEERTDDTWADELAVVQ
                     RQTESLSHPELGDLLRDLDRTARSASAMAASVTGLADYWFATMQGEDRVEASRVRLGI
                     "
     gene            complement(804..877)
                     /locus_tag="VNQ33_09890"
     tRNA            complement(804..877)
                     /locus_tag="VNQ33_09890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Gly,seq:tcc)
     gene            complement(934..2577)
                     /locus_tag="VNQ33_09895"
     CDS             complement(934..2577)
                     /locus_tag="VNQ33_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWJ98459.1"
                     /translation="MTVTLPRRTARRTEAPPVDLAEHGIHRDPVNHDPLGSATFSQRL
                     VQRAAGLFNRGTTRRSFLTRTAVLGSALAVGPIDFILKPGSAYAYTCGTCSDGWTAFC
                     CTINSGKNSCPPNSFVAGWWKADNAAYCCGAARYIIDCNATCPTQCACRCAGGSCDGR
                     RTCCNQFRYGQCHTEIACYGPVVCRVATCTPPWRYDPSCTTSSATDNKTVNHGAPCLT
                     TDCGGSAIDKKYDALGGAGGFLRAPTAAERANSDKRGRMRTYQGGNIYWTSTTGAHEV
                     HGDILKEFGKQATVNGVLKYPTSDTLTSSDKKSRYNNFENGRIYYRGGIGTFTVPKPF
                     FAKHESLRGVHGSLGYPIRNVTTSADKKSRYQNYEHGRIYLQGSRVVEIHGAVYTKHE
                     SLQGVYGPLGYPISDLGAAGDNRGKAQWFEKGFIWYTPTTGAHGLWGKVNDRHIANGH
                     VKGYLRYPTSEPMPVGDGRGTYATFERGNIYASTTTAGFQVHGAVLDAYLNTYGGPQG
                     SLGYPTSELDPAGTSGGRFQQFEGGKLRYNANGTVTLVS"
     gene            complement(2574..>3041)
                     /locus_tag="VNQ33_09900"
     CDS             complement(2574..>3041)
                     /locus_tag="VNQ33_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98460.1"
                     /translation="SHGIETDLPPGWEGRIGVRTQPSADTRAIGDTSEVPHPIVHLAN
                     FALPEQRGDFGSGAVDLMGDDDVLVVLFEYGPECANTALFKRQGIPKDLAPSMFSSSA
                     LQRTMPGQAGCQIFFTEAGRAFCSYTVLGRQSSALRVLPQANDTLSRTRIAPR"
BASE COUNT          418 a          960 c         1168 g          495 t
ORIGIN      
        1 cgatgccgag cctgacccgg gacgcctcga cgcggtcctc gccctgcatg gtggcgaacc
       61 agtagtcggc caggccggtc accgacgcgg ccatcgccga ggcggagcgt gcggtgcggt
      121 cgaggtcgcg cagcaggtcg ccgagctcgg ggtgcgagag cgactcggtc tggcgctgga
      181 ccacggccag ttcgtcggcc cacgtgtcgt cggtccgctc ctcgagcgtc gtgaagcggt
      241 cgatgacctc gacgtcggcg ccgtggcgga tctcgtcgcg cagggtgtcg aggtcctcgg
      301 gctcgaacgg gcggatccgc tcgggcatcg ctcggagctt ggccgcgacg tcagccaggt
      361 cgccgaagcg ctggccgtcc atgtccaaca gcttgaggac ggcctcgggg tcgagcccga
      421 gcgcctcgga ccggatctgg acctcgaccg ggatgcgggc cgacatcatc gccgtgcgga
      481 tgtccaccag ggcgagcagc ccgcccacct cgctgtgcac ggagccggcg acgtccgccg
      541 ctcggcgctt gtcggctgcg gtgtggccgg ccagggcccc ggccacgagc gtcgggacca
      601 agacgagggt gagcaaccgc cctcggagac ccacccggcg agttgcgatg gccatggacg
      661 tgctgctccg tggaaggccc cccaaggaag gatcggcccg gcgacggtgg aacttgaggg
      721 tttcgcccga acgttcgccg gtccgcgccg gcgtcgtcgt ccaagtcggg atcgtgccga
      781 cgaccgcctg ggtcccggtg cagtggagcg ggtgaaggga atcgaaccct cgtcatcagg
      841 atggaagcct gaggctctgc cattgagcta cacccgcaga gcgaccaacc gtactggtcc
      901 cgaccgatcc gccggcaacc agatcgtgac gggtcaagac accagggtca cggtgccgtt
      961 ggcgttgtag cgcagcttgc cgccctcgaa ctgctggaac cgcccgccgc tggtgccggc
     1021 gggatcgagc tccgaggtcg gatagccgag gctgccctgc ggaccgccgt aggtgttcag
     1081 gtaggcgtcg agcacggcgc cgtggacttg gaagccggcg gtcgtggtgc tggcgtagat
     1141 gttcccgcgc tcgaaggtgg cgtaggtgcc ccggccgtcg ccgacgggca tgggctcgga
     1201 ggtggggtag cggaggtagc ccttcacgtg cccgttggcg atgtggcggt cgttgacctt
     1261 gccccacagg ccgtgcgccc cggtggtcgg cgtgtaccag atgaagccct tctcgaacca
     1321 ctgggccttg ccccggttat cacccgcggc gccgaggtcc gagatcgggt agccgagggg
     1381 cccgtacacg ccctggagcg actcgtgctt cgtgtacacg gcgccgtgga tctcgaccac
     1441 gcggctgccc tgcaggtaga tccggccgtg ctcgtagttc tggtaccggc tcttcttgtc
     1501 ggccgacgtc gtcacgttcc ggatcgggta gccgagggag ccgtggacgc cgcgcagcga
     1561 ctcgtgcttg gcgaagaagg gcttggggac cgtgaaggtg ccgatgccgc cccggtagta
     1621 gatgcggccg ttctcgaagt tgttgtaccg gctcttcttg tccgacgacg tcagcgtgtc
     1681 ggacgtgggg tacttcaaga cgccgttgac ggtggcctgc ttgccgaact ccttgaggat
     1741 gtcgccgtgc acctcgtggg cgccggtggt ggacgtccag tagatgttgc cgccctggta
     1801 ggtgcgcatc cggccccgct tgtcggagtt ggcccgctcc gcagcggtgg gggcgcgcaa
     1861 gaagccgccg gcgccgccga gggcgtcgta cttcttgtcg atcgcggatc cgccgcagtc
     1921 ggtggtgagg cagggggcgc cgtggttcac ggtcttgttg tcggtggcgc tcgaggtggt
     1981 gcagctcggg tcgtagcgcc acggcggcgt gcaggtggcg acgcggcaga ccacgggccc
     2041 gtagcaggcg atctcggtgt ggcactggcc gtagcggaac tggttgcagc aggtgcggcg
     2101 gccgtcgcac gagccgccag cgcagcggca cgcgcactgg gtggggcagg ttgcgttgca
     2161 gtcgatgatg tagcgggcgg cgccgcagca gtaggcggcg ttgtcggcct tccaccaccc
     2221 ggccacgaag ctgttcggcg ggcacgagtt cttgccgctg ttgatggtgc agcagaaggc
     2281 ggtccacccg tccgagcagg tgccgcacgt gtaggcgtag gccgaaccgg gcttgaggat
     2341 gaagtcgatg ggacccacgg cgagcgcgga cccgagcacg gccgtgcggg tgaggaacga
     2401 gcggcgcgtg gtgcccctgt tgaacaagcc tgccgcccgc tgcacgaggc gctggctgaa
     2461 cgtcgccgat ccgagcgggt cgtggttcac cgggtcccgg tggatgccgt gctcggcgag
     2521 gtcgaccggt ggggcctcgg ttcggcgggc ggtgcgtcgg gggagcgtga ccgtcatcgg
     2581 ggagcgatcc tcgttcggga gagggtgtcg ttggcctggg gcagaacacg gagcgcggag
     2641 gactggcggc cgagcacggt gtaggagcag aacgccctcc cggcttccgt gaagaagatc
     2701 tggcagccgg cctggcctgg catcgtgcgc tgcagcgccg agctggagaa catcgacggc
     2761 gccaggtcct tcgggatgcc ctggcgcttg aagagggccg tgttcgcgca ctcggggccg
     2821 tactcgaaga ggacgacgag cacgtcgtcg tcgcccatga ggtcgacggc gccggagccg
     2881 aagtcgcccc gctgctcggg gagggcgaag ttggccaggt gcacgatcgg gtggggcacc
     2941 tcgctcgtgt cgccgatggc ccgggtgtcg gccgatggct gggtccggac cccgatcctt
     3001 ccctcccagc cgggcggcag gtcggtctcg atgccgtgtg a
//
LOCUS       DATMFS010000525         4810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_18484, whole genome shotgun sequence.
ACCESSION   DATMFS010000525 DATMFS010000000
VERSION     DATMFS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18484"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VNQ33_09905"
     CDS             <1..220
                     /locus_tag="VNQ33_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98461.1"
                     /translation="LGGLGGIDPSVAAHVGDLRRTDIAGAQGRGVFAVRYSGIYDDPG
                     NADDGSDQVEGDAVVADHADLLAALGIA"
     gene            265..1500
                     /locus_tag="VNQ33_09910"
     CDS             265..1500
                     /locus_tag="VNQ33_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ98462.1"
                     /translation="MVDPLELAAPDRYAVDGFPHEAWTELRATDPVHWTEPPGYRPFW
                     SITKHADLKHVSSHPELFSSRPRLTMQPIAMEEEMLRQFPDAADQGGMPLRMLVTMDP
                     PEHRAYRNLAGPYFRPSVLRNLEDRVEEITTLLLDRFAGTDVELDFVTDVASWHPLRM
                     ICEILGVASEREGLILRMTNELFGADDPELGREDQENLLPEMFEFFFNLVEEKRAQPG
                     EDLASVLANGEIDGEPLPHIELLSYFVLVSTAGHDTTRNALGSGMHALLHHPDQLALL
                     REDRSLVKTAADEMVRWASPVIHFIRTATEDTEVGGKAIKAGDHLVLFYPSANRDEDV
                     FEDPFSFRVDRDPNPHLGFGVGEHFCLGAGLARMEIRVFLEQWLDRIESVDLTGEPAH
                     TAASFVGGYKHLPLRWTLR"
     gene            complement(1634..2530)
                     /locus_tag="VNQ33_09915"
     CDS             complement(1634..2530)
                     /locus_tag="VNQ33_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWJ98463.1"
                     /translation="MAHPGIDVIRQLVGAVRHQGASIPERRASLAALAGSPPAPDGVA
                     VEAIELADRPAERLTPAGAPSGDVVLYLHGGSYHSGSPDTHRGLAGALALAAGRTVVT
                     LDYRLAPEDPFPAGLNDALLAFDQLAPARVALAGDSAGGGLAAATALALRDRAGGQPV
                     AVALISPWTDLTQSAGSYTSRAAADPMLTAAALDQAAEAYLAGTDPRTSYVSPVFGDL
                     AGLPPVRVDVGADEVLFDDSQTLVEGITAAGGDAVLVEWPEMIHVFQAFPPDLVPEAA
                     HAVAAMGAFLAGHFDASRDRGA"
     gene            2558..4477
                     /locus_tag="VNQ33_09920"
     CDS             2558..4477
                     /locus_tag="VNQ33_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWJ98464.1"
                     /translation="MSDRYAGLPIRDDDAAIAAALEDVSVPTLLVSLVHLTGDSSILR
                     GPLRPAGIYLNEVQGFMSPEDQAAARAFALEKIIEFRDGGCVLPPPPSPEVIKEMMAF
                     LIAGAIPDEYVPMMLEELALHGDDERDVDLSEIDPEAKAAFHVVVIGAGMSGLLAGIR
                     LAAAGIGFTIIEKNADVGGTWFENRYPGCRVDVGNHFYSYSFAPDDGWTEFFAQQPEL
                     QAYFHRCMVDHGIADHIRFETEVRSATWGEATGTWTVETDDGRQLTANAVISAVGQLN
                     RPKLPDIVGRASFEGLAMHSAEWVDGTDLRGQRVAVVGTGASAFQIVPTIAADVEHLT
                     VFQRSAPWMFPNPNYHEQVGPGVQWAIQHLPYYGRWYRFLLFWPACDGGMVAMTKDPD
                     YADPEHGISDINDAAKEMFTQWMRDQVGDDEELFAKVVPDYVCLGKRTLQDNGSWLTA
                     LTRPNVDLVTEPIAEVTPTGIRTADGTDRDVDVIVYATGFHANRYLWPMDVVGRDGIV
                     LGEQWGERPTALLGITVPNFPNLFCLYGPGTNLASGGSLIFHSECQVRYVMGCLATLL
                     GDGVSSMEVRQDAHDEYNERLQARMREMVWSHPSIKTSWYRNSEGETYILSPWRLVDY
                     WAWTQAPDPDHFEVR"
BASE COUNT          708 a         1758 c         1670 g          674 t
ORIGIN      
        1 cctcggcggg ctgggcggca tcgacccgtc ggtggcggcc cacgtgggcg acctccgccg
       61 caccgacatc gccggtgccc agggccgtgg cgtcttcgcc gtccgctact cgggcatcta
      121 cgacgatccc ggcaacgccg acgacggctc ggaccaggtc gaaggcgacg ctgtcgtcgc
      181 cgaccacgcc gacctcctcg ccgccctcgg gatcgcctga tcgggcctcc tgatcccgca
      241 gatgatctgc ggcaggctgg ggtgatggtc gatccactcg aactggccgc tccggaccgc
      301 tacgccgtcg acgggttccc gcacgaggcg tggaccgagc tgcgggccac cgatccggtg
      361 cactggaccg aaccgccggg ctaccggccg ttctggtcca tcaccaagca cgccgacctg
      421 aagcacgtct cgtcgcaccc ggagctgttc tcgtcgcgcc cgcggctcac catgcagccc
      481 atcgcgatgg aagaggagat gctccgccag ttccccgacg ctgccgacca gggcgggatg
      541 ccgctgcgga tgctggtcac gatggacccg cccgagcacc gggcgtaccg caacctcgcc
      601 ggcccgtact tccggcccag cgtgctgcgg aacctggagg accgggtcga ggagatcacc
      661 acgctgctgc tcgaccgctt cgccggtacc gacgtggagc tggacttcgt caccgacgtg
      721 gcgtcgtggc acccgctccg catgatctgc gagatcctcg gtgtcgcctc ggagcgcgag
      781 ggcctgatcc tgcggatgac caacgagctg ttcggcgccg acgaccccga gctcggccgg
      841 gaggaccagg agaacctcct gccggagatg ttcgagttct tcttcaacct ggtcgaggag
      901 aagcgggccc agccgggtga ggacctggcg tcggtgctgg ccaacggcga gatcgacggc
      961 gaaccgctcc cgcacatcga gctgctcagc tacttcgtgc tggtctcgac cgccggccac
     1021 gacaccaccc gcaacgccct cggctcgggc atgcacgccc tgctgcacca cccggaccag
     1081 ctcgccctcc tgcgcgagga ccgatcgctc gtgaagaccg ccgccgacga gatggtccgc
     1141 tgggccagcc cggtcatcca cttcatccgc accgccaccg aagacacgga ggtcggcggc
     1201 aaggcgatca aggccggcga ccacctcgtg ctcttctacc cgtcggccaa ccgcgacgag
     1261 gacgtgttcg aggacccgtt ctcgttccgg gtggaccggg acccgaaccc gcacctcggc
     1321 ttcggggtgg gcgagcactt ctgcctgggg gccggcctgg cccgcatgga gatccgcgtc
     1381 ttcctcgagc agtggctcga ccgcatcgag tccgtcgatc tcaccggcga accggcccac
     1441 accgccgcca gcttcgtcgg cggctacaag cacctccccc tccgctggac cctccggtag
     1501 cgaccggatc cgccacccgc cgccgaactt cgcatgcgag agcgcccgtt cgggagcggt
     1561 gacctacgaa gttcgccggg gcccgaggcc cagggcttgg gtcctttggc gtggcggctg
     1621 cggtaccagc ccgtcaggcg cctcggtccc tgctggcgtc gaagtgcccg gcgaggaagg
     1681 cacccatcgc cgccactgcg tgggcggcct cgggcacgag gtccggtggg aaggcctgga
     1741 acacgtggat catctcgggc cactcgacga gcacggcgtc gccaccggcg gccgtgatcc
     1801 cctcgacgag ggtctggctg tcgtcgaaca gcacctcgtc ggcccccacg tcgacgcgca
     1861 cgggtggcag tccggccagg tcgccgaaca cgggcgagac gtaggaggtg cggggatcgg
     1921 tgccggcgag ataggcctcg gccgcctggt cgagggcggc ggccgtgagc atcgggtcgg
     1981 ccgcggcgcg agacgtgtac gacccggccg actgcgtgag gtcggtccac ggcgagatga
     2041 gggcgacggc gaccggctgg cctccggcgc ggtcgcgcag ggcgagcgcg gtggccgcgg
     2101 cgaggccgcc gccggccgag tcgccggcga gggcgacgcg tgccggtgcc agctggtcga
     2161 acgcgaggag ggcgtcgttg agcccagccg ggaaggggtc ctccggggcg agccggtagt
     2221 cgagcgtgac gaccgtgcga ccggccgcga gggcgagggc gccggccagc ccgcggtgcg
     2281 tatcgggcga gccggagtgg tagctgccgc cgtgcaggta gagcacgacg tcgccgctgg
     2341 gcgcccccgc tggggtcagc cgctcagccg ggcggtcggc cagctcgatg gcctcgaccg
     2401 ccacgccatc gggtgctggc ggcgagccgg ccagcgcggc gaggctcgcc cgccgctccg
     2461 ggatcgacgc cccctggtgc ctcacggcgc cgacgagttg gcggatgacg tcgattccgg
     2521 ggtgggccat ggctcggcat cgtagggtcc ggccctgatg agcgaccgat acgccggact
     2581 cccgatccgc gatgacgatg ccgccatcgc ggcggccctc gaggacgtga gcgtgcccac
     2641 cctcctcgtc tcgctggtgc acctcaccgg ggactcgtcg atcctgcgcg gccccctccg
     2701 gcccgcgggc atctacctca acgaggtgca gggcttcatg agccccgagg accaggccgc
     2761 ggcccgtgcc ttcgccctcg agaagatcat cgagttccgc gacggcgggt gcgtcctgcc
     2821 gccgccgccg tcgcctgagg tgatcaagga gatgatggcg ttcctcatcg ccggagcgat
     2881 ccccgacgag tacgtgccga tgatgctcga ggagctggcg ctgcacggcg acgacgagcg
     2941 cgacgtcgac ctgagcgaga tcgacccgga ggccaaggcc gcgttccacg tcgtggtcat
     3001 cggcgccggc atgtcgggcc tgctcgccgg gatccgcctg gctgcggccg gcatcgggtt
     3061 caccatcatc gagaagaacg ccgatgtggg cggcacctgg ttcgagaacc gctacccggg
     3121 ctgccgggtc gacgtgggca accacttcta ctcgtactcc ttcgcccccg acgacggttg
     3181 gaccgagttc ttcgcccagc agcccgagct ccaggcgtac ttccaccggt gcatggtcga
     3241 ccacggcatc gccgaccaca tccgcttcga gaccgaggtc cgctcggcca cgtggggcga
     3301 ggccaccggc acgtggaccg tcgagaccga cgatggccgg cagctcaccg ccaacgccgt
     3361 catcagcgcg gtcggccagc tcaaccggcc caagctcccc gacatcgtcg gccgcgcatc
     3421 gttcgaaggc ctggccatgc actcggcgga gtgggtcgac ggcaccgacc tgcggggcca
     3481 gcgggtcgcc gtcgtcggca ccggcgcgag cgcgttccag atcgtgccga ccatcgccgc
     3541 cgacgtcgag cacctcaccg tgttccagcg gtcggcgccg tggatgttcc ccaacccgaa
     3601 ctaccacgag caggtcgggc ccggcgtcca gtgggcgatc cagcacctgc cgtactacgg
     3661 gcgctggtac cggttcctcc tgttctggcc ggcgtgcgac ggcgggatgg tcgccatgac
     3721 gaaggacccg gactacgccg atcccgagca cggcatcagc gacatcaacg acgccgccaa
     3781 ggagatgttc acccagtgga tgcgcgacca ggtcggtgac gacgaggagc tgttcgccaa
     3841 ggtcgtgccg gactacgtct gcctcgggaa gcggaccctc caggacaacg gcagctggct
     3901 gaccgcgctc acccgcccga acgtggacct ggtgaccgag cccatcgccg aggtcacgcc
     3961 caccggcatc cgcaccgccg acggcaccga ccgcgacgtc gacgtgatcg tctacgccac
     4021 cggcttccac gccaaccgct acctctggcc gatggacgtc gtcggccgcg acggcatcgt
     4081 gctgggcgag cagtgggggg agcgcccgac cgcgctgctc ggcatcaccg tgcccaactt
     4141 ccccaacctc ttctgcctct acggccccgg caccaacctg gcgagcggcg gcagcctgat
     4201 cttccactcc gagtgccagg tccggtacgt gatgggctgc ctcgccaccc tgctgggcga
     4261 cggcgtgtcg tcgatggagg tccgccaaga cgcccacgac gagtacaacg agcgcctcca
     4321 ggcccgcatg cgggagatgg tctggagcca cccgagcatc aagaccagct ggtaccgcaa
     4381 cagcgagggc gagacctaca tcctcagccc ctggcgcctc gtggactact gggcctggac
     4441 ccaggccccc gaccccgatc acttcgaggt gcgctgagcc ggacgtccca tgatcgagcg
     4501 gcggatcgcg gcgacggtac ggccgcctcg tcaccgaggc gccggtcggc cggcggggga
     4561 ctcggcaggg gactcggcga gcccgcgggg gacggtcctc ggaagatctg cccgctcgtc
     4621 cgtcagagaa cggcgcctcg accgctctga aggggcgtgg atccagacag gagggcgcgg
     4681 tgggcggtcg ccgcggccct tctcggcgtc gtggccctgg agctggtggc cacgcgccac
     4741 ctgctgcgtc tcgtgtcgtg gccgaacgac ctctcgttcc accactccat ggtcgactgg
     4801 gccggcggcc
//
LOCUS       DATMFS010000526         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_271862, whole genome shotgun sequence.
ACCESSION   DATMFS010000526 DATMFS010000000
VERSION     DATMFS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271862"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..559)
                     /locus_tag="VNQ33_09925"
     CDS             complement(68..559)
                     /locus_tag="VNQ33_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HWJ98465.1"
                     /translation="MSDAPQGPGWWQASDGKWYPPTAQPGTPRPPAAPGPSPYGPGPA
                     PYGPGAAPYGPGPAPYGPGPAPYGYGYAPNPNGTPSGLPSVNGFATASLVLGIFSLVI
                     CCAPFMLCSLAGLPLGIYALTRINKRTADPGPRGLAITGVVVNGIGFAILAIVAVAAV
                     AGS"
     gene            738..>3178
                     /locus_tag="VNQ33_09930"
     CDS             738..>3178
                     /locus_tag="VNQ33_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HWJ98466.1"
                     /translation="MFDDLKIRNKLILLMAGPIAIILLLSALGAKDRRESAAASRDVA
                     KLVTVARLDSDVADALQQEALYSSAYVGSGQKAWAKELKAARANTDTVMGQAVPVLQD
                     GLGGTDEGIRSAAGLAVGAADKLGFYRKSIDQGFEPEQFNEVVVSYGDLQNTFLAVNT
                     NIGDTMSDPQASAELRSAAALGAYKASLARQGALVAGAAETESKSFGSQKTFDLFQKA
                     QTSERSQLALLNSLSGLNRKGAVRNALASDQSQVFDTIREKAADAGAAGDLGFDGKVV
                     VTATGTVIKDLHQVESDLLAQLITDSTAARESAERASTLFLVAALVAIGGAVLAALFL
                     GRRITKPLGKLTEAADRLSGEQMPRLIETLKNPTEDELGMSIGAIAPIEIDSNDEIGQ
                     LARSFNEVQRVASEVANEQAQLLRKGIGEMFVNLARRNQALLDRQIEFIDELERGEED
                     PDQLDNLYRLDHLATRMRRNAESLLVLAGAEPPRRRGRPAPLANVVRAALAEVEDFGR
                     IELLSFDEVLVASNAAADLAHLLSELMENATNFSPPETRVEVVGHKTKADGYVISVTD
                     HGIGMSAEQIAEANDSLARPPLVGLAMSRSLGFIVVGRLASRHGIAVRMMPSAAGGVT
                     AVVSIPPALVVDTPGGMSEPQPVVEAPARLKPVLDSPTTGLAPLTFNPADTGAESAAD
                     APAAAPDAAPVPGAPPAPVPAGDGPALFAPPTPSAPAGSGSGELAAWPADPPAPVTAA
                     TDEAALPSRSIFDTPPPAAVDPAPGAEPAPMADDEVTAAIAAQVDEAVGPAMPAALSF
                     DAPDPERTPSAPIPPS"
BASE COUNT          465 a         1190 c         1111 g          412 t
ORIGIN      
        1 cgcggcccgg tccgggtcgg gcggtggcgg gaggtgctgg taggcgagcg atgccatcag
       61 gtgctcctca cgacccggcc accgcggcca cggcgacgat cgccaagatg gcgaacccga
      121 tcccgttgac gaccacgccg gtgatggcga ggccccgggg acccgggtcc gcggtgcgct
      181 tgttgatcct ggtcagggca tagatcccga gggggagccc ggcgagcgag cagagcatga
      241 agggagcgca gcagatgacc aggctgaaga tgccgagcac cagggacgcg gtggcgaagc
      301 cgttcaccga cggcaggccg ctcggcgtgc cgttggggtt cggcgcgtag ccgtacccat
      361 agggtgcggg acccggcccg tagggcgccg gtccggggcc gtacggagcg gcaccgggtc
      421 cgtacggagc ggggccgggg ccgtagggcg atgggcccgg cgcggccggc gggcgcggcg
      481 tgccgggctg ggcggtgggc ggataccact tgccatcgga tgcctgccac caaccggggc
      541 cctggggtgc gtcgctcatg cggtgccagt ctgcccgctg ggctggggag cggaggaccc
      601 gcctgagcgc gactgggtcg ttcgacccat ctagggcata cggcccaccc gctgctcaag
      661 accgggcgga atcggccgat ctgacctctc gaccagtgca cccccgccgg ccgattccct
      721 tctcccacga ggacgccgtg ttcgacgatc tgaagatccg caacaagctg atcctgctga
      781 tggcagggcc gatcgcgatc atcctgctcc tctccgcgct cggcgcgaag gaccgccggg
      841 agtcggcagc cgccagccgc gacgtcgcca agttggtgac ggtcgcccgg ctggacagcg
      901 acgtcgccga cgccctgcag caagaagcgc tctacagctc ggcgtacgtg gggtccggcc
      961 agaaggcctg ggcgaaggag ctgaaggccg ctcgggccaa caccgacacg gtgatgggcc
     1021 aggccgtgcc cgtcctccaa gacggcctgg gcgggaccga cgagggcatc cggtccgcgg
     1081 ccgggctcgc cgtgggcgcg gccgacaagc tgggcttcta ccgcaagtcg atcgaccagg
     1141 gcttcgagcc cgagcagttc aacgaggtgg tggtcagcta cggcgacctc cagaacacct
     1201 tcctcgccgt gaacaccaac atcggcgaca cgatgtccga cccgcaggcg tccgccgagc
     1261 tccgttccgc cgccgcgctg ggcgcctaca aggcctccct cgcccggcag ggcgcgctgg
     1321 tcgccggcgc cgccgagacg gagtccaagt ccttcggctc ccagaagacc ttcgacctct
     1381 tccagaaggc ccagacgagc gagcgcagcc agctcgccct cctcaactcc ctctctgggc
     1441 tgaaccgcaa gggcgccgtc cgcaacgccc tggcgtccga tcagagccag gtgttcgaca
     1501 ccatccgcga gaaggcggcc gacgcgggtg ccgcaggcga cctcggcttc gacggcaagg
     1561 tcgtggtcac ggccacgggg accgtcatca aggacctcca ccaggtggag tccgatctgc
     1621 tcgcccagct catcaccgac agcaccgccg cccgcgagag cgccgagcgg gcatcgacct
     1681 tgttcctcgt cgccgccctg gtggccatcg gcggcgccgt gttggctgcc ctcttcctcg
     1741 gccgccggat caccaagccg ctgggcaagc tgaccgaggc ggccgaccgc ctgtcgggtg
     1801 agcagatgcc ccgcctcatc gagacgctga agaaccccac ggaagacgag ctgggcatgt
     1861 cgatcggcgc catcgccccg atcgagatcg acagcaacga cgagatcggc cagctggccc
     1921 ggtcgttcaa cgaggtccag cgagtggcca gcgaggtcgc caacgagcag gcccagctcc
     1981 tgcgcaaggg catcggcgag atgttcgtga acctcgcccg ccgcaaccag gccctgctcg
     2041 accgccagat cgagttcatc gacgagctcg agcgcggcga ggaggatccc gaccagctcg
     2101 acaacctcta ccgcctcgac cacctcgcca cccgcatgcg ccgcaacgcc gagtcgctgc
     2161 tcgtgctggc cggggccgag ccgccccgtc gccggggccg tccggcgccg ctcgccaacg
     2221 tggtgcgtgc cgcgctcgcc gaggtcgagg acttcggccg catcgagctg ctctcgttcg
     2281 acgaggtgct cgtcgcgtcg aacgccgcgg ccgacttggc ccacctgctc tccgagctca
     2341 tggagaacgc caccaacttc tcgccgcccg agacccgggt cgaggtcgtc ggccacaaga
     2401 ccaaggccga tggctacgtc atctcggtga ccgaccacgg catcggcatg tcggccgagc
     2461 agatcgccga ggccaacgac tccttggccc gtccgccgct cgtcggcttg gccatgtccc
     2521 gctcgctcgg cttcatcgtg gtcggccggc tggcctcccg tcacgggatc gcggtgcgca
     2581 tgatgccgtc ggctgccggt ggcgtgaccg cggtggtctc gatcccgccc gcgctcgtgg
     2641 tcgacacgcc aggaggcatg agcgagcccc agcccgtcgt cgaggcgccg gcccggctca
     2701 agccggtgct cgacagcccc acgacgggcc tggcgccgct cacgttcaac ccggccgaca
     2761 ccggcgccga gtcggcggcg gacgcaccag ccgcggcgcc ggacgcagcg ccggtcccgg
     2821 gcgctccgcc cgcgccggtg ccggcgggcg acggtcccgc cctcttcgct ccgccgacgc
     2881 cgtcggcgcc ggcgggcagc ggttcgggcg agctggcggc gtggccggcc gatccgccgg
     2941 cgccggtcac ggctgccacc gacgaagcgg cgctcccgag ccgctccatc ttcgacacgc
     3001 cgccaccggc cgccgtcgat cccgccccag gcgccgaacc ggcgccgatg gccgacgacg
     3061 aggtcacggc ggccatcgcc gcccaggtgg acgaggccgt cggcccggcg atgcccgcgg
     3121 cgctgtcgtt cgacgctccc gaccccgagc ggactccgtc ggccccgatc ccgccgtc
//
LOCUS       DATMFS010000527         3044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_18614, whole genome shotgun sequence.
ACCESSION   DATMFS010000527 DATMFS010000000
VERSION     DATMFS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18614"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1481)
                     /locus_tag="VNQ33_09935"
     CDS             complement(84..1481)
                     /locus_tag="VNQ33_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98467.1"
                     /translation="MTRNPPSPTLDAPISTVPSVSAAELAAHTPAGRNRAVDLYRAAA
                     MAVVAVGHWLGTVVVVHRGKLGGGNLLDYAPEYGWFTWIGQVMPLFFFVGGFASATSL
                     RSADKRGVRPADWVATRLRRMVAPAAALAAFWAVALAIGATVGGFGIVSLAAAGAAIP
                     LWFLANYTVDTALAPFTFRWFRARPVVFTASLLGLFAVAEAAGFAGIRFVPQLNWVIG
                     WLGFQVAGFAWQDGRLPAGRGLVALAAAFWALALAAVNLGPWPAVMLHHGGLEHSPTH
                     PPSTALLLFGFAYSFTAAALAPKVNRWLEQSARAWKVTIAANSVAMSVYLWHMTAGMV
                     VIGAAYLAGLIPDIEAGTSAWWWTKIPFLLANIAVLVPIVRKVAPIEQRQLLAGPSTW
                     RWGTGSMLAVAALLSASIKAWSSPHAPVLAGGVVGTLAIWHLVLRDRPPSTGLVEAPA
                     PARQDEGADGERAQA"
     gene            1652..2098
                     /locus_tag="VNQ33_09940"
     CDS             1652..2098
                     /locus_tag="VNQ33_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWJ98468.1"
                     /translation="MSDSPAPAPADLLRSHGLHVTAQRLAVLRAVAERPHGTADGVID
                     AVRTEIGAVSRQAVYDSLAVLVDKGVLRRIQPAGLSARYEQRVGDNHHHLVCRDCGRI
                     VDVDCATGEAPCLHAAEDWGFEIDEAEVIYWGRCPDCLAAATAAAT"
     gene            2160..>3044
                     /locus_tag="VNQ33_09945"
     CDS             2160..>3044
                     /locus_tag="VNQ33_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWJ98469.1"
                     /translation="MSNDERDTVEEHRFDDEHEIDNEHGTVTTSDEPSDARCPVMHAG
                     HQAVGSAANQHWWPDQLNLRILRQNHPDADPLGDFDYKAAFEALDHEALAADIDAIMT
                     DSQPWWPADYGHYGPFFIRMSWHAAGTYRVADGRGGGGAGAQRFAPLNSWPDNVSLDK
                     ARRLLWPVKQKYGQAISWADLLVLAGNRALETMGFTTFGFAGGRRDIWSPEDDVYWGP
                     EDVWLGDERYSGDRELANPLGAVQMGLIYVNPEGPNGNPDPMASARDVRETFGRMAMD
                     DEETVALIAGGHTFGKMHG"
BASE COUNT          484 a         1109 c         1112 g          339 t
ORIGIN      
        1 gcaccgatcc gccgggctcg gcgccgtcag gcggcccctc gccgctgccc cgcacgggaa
       61 gcgaccccgt gccgctcgtg gtctcaggcc tgggcgcgct cgccgtcggc gccctcgtcc
      121 tggcgggccg gcgccggcgc ctccaccaga ccggttgacg gcggccgatc ccggaggacc
      181 aggtgccaga tggcgagcgt gccgacgacc ccgccggcca ggaccggcgc gtgcgggctg
      241 gaccacgcct tgatgctggc ggacagcagg gcggcgaccg ccagcatcga gccggtgccc
      301 cagcgccacg tcgacggacc ggccaggagc tggcgctgct cgatcggggc caccttccgc
      361 acgatcggga cgaggacggc gatgttggcg aggaggaacg ggatcttcgt ccaccaccag
      421 gcggaggtcc cggcctcgat gtcggggatc aggccggcga ggtaggccgc accgatcacg
      481 accatcccgg cggtcatgtg ccagaggtac acggacatgg caacggagtt ggcggcgatg
      541 gtgaccttcc aggcccgggc cgactgttcc agccagcggt tcaccttcgg ggccagcgcc
      601 gccgccgtga acgagtaggc gaagccgaac agcagcagcg ccgtcgacgg cgggtgggtg
      661 gggctgtgct ccagcccgcc gtggtggagc atgaccgccg gccacgggcc gaggttcacc
      721 gcggcgagcg ccagcgccca gaacgccgcc gccagggcca ccagcccacg ccccgccggg
      781 agcctgccgt cttgccaggc gaagccggcg acctggaagc ccagccagcc gatgacccag
      841 ttcagctggg gcacgaaccg gataccggcg aagccggcgg cctcggcgac ggcgaacagc
      901 ccgagcagcg aggcggtgaa caccaccggc cgggcccgga accaccggaa ggtgaacgga
      961 gcgagcgcgg tgtcgaccgt gtagttggcg aggaaccaca gcgggatcgc cgcgcccgcc
     1021 gcggcgagcg acacgatgcc gaagccgccg acggtggcac cgatcgccag tgccaccgcc
     1081 cagaacgcgg cgagggccgc tgcgggggcg accatccggc gcagccgggt cgccacccag
     1141 tcggctggac gcacgccccg cttgtccgcc gaccgcagcg aggtggcgga ggcgaagccg
     1201 ccgacgaaga agaacagcgg catcacctga ccgatccagg tgaaccagcc gtactcgggc
     1261 gcgtagtcga ggaggttgcc gccgccgagc ttgccgcggt gcaccacgac gacggtcccg
     1321 agccagtggc cgacggccac gaccgccatg gcggctgctc ggtagaggtc gacggcgcgg
     1381 ttgcggccgg cgggggtgtg ggcggcgagc tcggcggccg agacggaggg gaccgtcgag
     1441 atgggcgcgt cgagcgtggg ggacggcggg ttgcgggtca tgggcccatt ccgccagggc
     1501 cggccgcacc gggccccggg gagaaccccc caatccggac cgggcggccc ggtggggctc
     1561 ggccgctcgc ccgactcccg ggagccggcg ctccgtgaac ggaccgagac gacttgttga
     1621 cagtgtccag aagtaggcag acgatggctt catgtccgat tcgccggcgc cggcaccagc
     1681 cgacctgctg cggtcccacg gcctgcacgt cacggcccag cgcctcgcgg tgctgcgggc
     1741 ggtggccgaa cgcccgcacg gcaccgccga cggcgtcatc gacgccgtgc ggaccgagat
     1801 cggcgcggtg tcgcgccagg cggtctacga ctcgctcgcc gtcctcgtcg acaagggcgt
     1861 cctgcggcgc atccagcccg ccggcctctc cgcccgctac gagcagcggg tgggtgacaa
     1921 ccaccaccac ctcgtgtgcc gcgactgcgg ccgcatcgtc gacgtcgact gcgcaaccgg
     1981 cgaggcgccg tgcctgcacg cggccgagga ctggggcttc gagatcgacg aggccgaggt
     2041 catctactgg ggtcgctgcc ccgactgcct cgcggcggcg acggcggctg ccacctgatc
     2101 acggcgagaa gacgagaaga accgaccacc cacctcactt caaccaccaa ggggaaccga
     2161 tgagcaacga cgagcgcgac accgtcgagg agcaccgctt cgacgacgag cacgagatcg
     2221 acaacgagca cggcaccgtc accaccagcg acgagccgtc cgacgcccgc tgcccggtga
     2281 tgcacgccgg gcaccaggcc gtcggttcgg ctgcgaacca gcactggtgg cccgaccagc
     2341 tgaacctgcg catcctccgc cagaaccacc ccgacgccga cccgctcggg gacttcgact
     2401 acaaggccgc cttcgaggcc ctcgaccacg aggcgctggc ggccgacatc gacgccatca
     2461 tgacggactc ccagccgtgg tggccggcgg actacggcca ctacggcccg ttcttcatcc
     2521 ggatgagctg gcacgctgcg ggcacctacc gggtggcgga cggccgcggc ggcggtggcg
     2581 ccggcgccca gcggttcgcc ccgctcaaca gctggcccga caacgtgagc ttggacaagg
     2641 cccgccgcct gctgtggccc gtcaagcaga agtacggcca ggccatctcg tgggccgacc
     2701 tgctggtgct cgccggcaac cgggcgctgg agaccatggg cttcacgacc ttcggcttcg
     2761 ccggcggccg tcgcgacatc tggtcgccgg aggacgacgt gtactggggc cccgaggacg
     2821 tgtggctcgg cgacgagcgc tactcgggcg accgggagct ggccaacccg ctgggcgcgg
     2881 tgcagatggg cctgatctac gtgaaccccg agggcccgaa cggcaacccg gacccgatgg
     2941 cctcggcccg cgacgtgcgc gagacgttcg gccgcatggc catggacgac gaggagacgg
     3001 tggcgctcat cgccggcggc cacaccttcg gcaagatgca cggc
//
LOCUS       DATMFS010000528         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_113048, whole genome shotgun sequence.
ACCESSION   DATMFS010000528 DATMFS010000000
VERSION     DATMFS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113048"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VNQ33_09950"
     CDS             <1..1032
                     /locus_tag="VNQ33_09950"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HWJ98470.1"
                     /translation="DLLGCRYPIVQTGMGWVSGANLTAATSAAGGFGILASITMTDEQ
                     FRDAVASVKAKTDAPFGANFRAGQPDLDERIAFATKEGIGLFSFAGAPTRDAIAKIHD
                     AGGLVMPTVGARRHAEKMAEWGVDAVIAQGGEGGGHTGSVPTSLLLPQVVEAVGSQIP
                     VVGAGGFHSGQGLVAALAYGADGIAMGTRFLLTQESRVPESIKGDYTKATVFDTVVTT
                     ALDGAPQRVIRTELIDGLEKASIVTRMPKAALKALEFRKQTGTSLKELWAEGMAMRKS
                     QDLTWAQLAMAANAPMLIKTALVDGDASVGVLPTGQVTGVIDDLPTVADLLEQIVADA
                     RATLTRLGA"
     gene            1167..>2293
                     /locus_tag="VNQ33_09955"
     CDS             1167..>2293
                     /locus_tag="VNQ33_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98471.1"
                     /translation="MDLTWSADEEAFRAEVRAWLETELASWRTRHDDRILSGDTTEGF
                     AQHLDWERTLFASRYAVVSWPEELGGRGASRWQWLIFEEEYYRAGGPQRVTQNGIFLL
                     APTVFEFGNEEQQAHILPRMAGALDLWCQGWSEPNAGSDLASLTCKATRDDERGGWVL
                     DGQKTWTTRGAFCSHLFGLFRTDPESERHKGLTYLLVPLDIEGITVRGFGRLDGDEGF
                     AEVFFDGAFLPDDAIPGGVVLGGIGQGWSVAMATTSSERGLTLRAPGRFTATADRLVD
                     LYRDRAKAAGPDGIDPGLRDRVVEGWMEAEAYRLQTLQTVTDDAEGRSAGAASSFVKL
                     WWSELDVKLNETALDILGPEAELDGPWSKGFQFALSGPIYAG"
BASE COUNT          335 a          814 c          824 g          320 t
ORIGIN      
        1 gatctgctgg ggtgccggta cccgatcgtg cagaccggca tggggtgggt ctcgggggcg
       61 aacctgacgg ctgccacgag tgcggcgggc gggttcggga tcctggcgtc gatcacgatg
      121 accgacgagc agttccgcga cgccgtcgcg tcggtcaagg cgaagaccga tgcgcccttc
      181 ggcgccaact tccgcgccgg ccagcccgac ctcgacgagc gcatcgcctt cgccacgaag
      241 gagggcatcg ggctgttctc gttcgccggg gcaccgacca gagacgccat cgccaagatc
      301 cacgacgccg gcggcctcgt catgcccacg gtcggcgccc ggcgccacgc cgagaagatg
      361 gccgagtggg gcgtcgacgc ggtcatcgcc caaggcggcg agggaggcgg ccacaccggc
      421 tcggtgccca cgtcgctcct cctgccccag gtggtggagg cggtcggctc gcagatcccg
      481 gtggtcggcg ccggcggctt ccactccggt cagggtctcg tcgcggcgct ggcgtacggc
      541 gccgacggca tcgccatggg aacccgcttc ctcctgacgc aggagagccg ggtgccggag
      601 tcgatcaagg gcgactacac gaaggcgacg gtgttcgaca ccgtcgtcac caccgcgctc
      661 gacggcgctc cgcagcgggt gatccgcacc gagctcatcg acggcttgga gaaggcgtcg
      721 atcgtcaccc gcatgcccaa ggcggcgttg aaggcactcg agttccgcaa gcagaccggc
      781 acgtcgctca aggagctgtg ggccgaaggc atggcgatgc gcaagagcca ggacctcacc
      841 tgggcccagc tggccatggc cgccaacgcc ccgatgctga tcaagacggc gctcgtcgac
      901 ggcgatgcct cggtcggcgt gctccccacc ggccaggtca ccggcgtcat cgacgacctc
      961 cccacggtgg ctgatctcct tgagcagatc gtcgccgacg cacgagccac cctcacccgc
     1021 ctcggcgcct gaacccgtcc tcgctcggcg ggaggggtgg gtgcaggagc cgctggtggg
     1081 cgacggtcgg tgcactcgcc ccgggacggg gcccgcgtgg tccggttcct ggatctgacg
     1141 gggtgtcaga tacgctcgct cgccgtatgg acctgacctg gagcgctgac gaggaagcct
     1201 tccgcgccga ggtgcgggcg tggctggaga ccgagctggc gtcgtggcgc acccgccacg
     1261 acgaccggat cctgagcggc gatacgacgg agggcttcgc ccagcacctc gactgggagc
     1321 ggaccctgtt cgcctcccgg tacgcggtgg tgtcgtggcc cgaggagctc ggcgggcgcg
     1381 gggccagccg gtggcagtgg ctcatcttcg aggaggagta ctaccgggcg ggcggccctc
     1441 agcgggtcac ccagaacggc atcttcctgc tggcgccgac ggtcttcgag ttcggaaacg
     1501 aggagcagca ggcccacatc ctcccccgca tggccggcgc cctggacctg tggtgccagg
     1561 gctggtccga gcccaacgcg gggtccgacc tggccagcct cacctgcaag gccacccgcg
     1621 acgacgagcg cggcggctgg gtgctcgacg gccagaagac ctggaccacc cgtggcgcct
     1681 tctgcagcca cctgttcggc ctcttccgga ccgacccgga gagcgagcgc cacaagggcc
     1741 tcacctacct gctggtcccg ctcgacatcg agggcatcac ggtccggggc ttcggccggc
     1801 tcgacggcga cgagggcttc gccgaggtgt tcttcgacgg cgccttcctc cccgacgacg
     1861 ccatccccgg cggcgtggtg ctgggcggca tcggccaggg gtggtcggtc gccatggcca
     1921 ccacatcgtc cgagcggggc ctcaccctgc gtgcgccggg gcggttcacc gccacggccg
     1981 accgcctcgt cgacctctac cgggatcggg ccaaggcagc agggcccgac ggcatcgacc
     2041 ccggcctgcg agaccgggtc gtggagggct ggatggaggc cgaggcctac cggctccaga
     2101 cgctccagac ggtcaccgat gacgccgagg ggcgcagcgc cggtgccgcc agcagcttcg
     2161 tgaagctgtg gtggtccgag ctcgacgtga agctcaacga gaccgcgctc gacatcttgg
     2221 gccccgaggc cgagctcgac gggccgtgga gcaagggctt ccagttcgcc ctctccggcc
     2281 ccatctacgc ggg
//
LOCUS       DATMFS010000529         2664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_344371, whole genome shotgun sequence.
ACCESSION   DATMFS010000529 DATMFS010000000
VERSION     DATMFS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344371"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VNQ33_09960"
     CDS             <1..605
                     /locus_tag="VNQ33_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWJ98472.1"
                     /translation="PLFGAIVAYVLGDHHALTRSRILGLVIGVAGVALVVGAAGGDGD
                     VRLVNVAEVLVVAVCYAVAPFITARKLTEVPGVGLAALALAMVAVGYIVPASLTQDQT
                     PTGRSIGALVALAVLCTAIAFVIFFALIREVGPARATVITFINPIVALALGVVVLDEH
                     LSWGQILGLPIVLAGCWLAAGRRPAEVEPAAELATPAPAD"
     gene            complement(673..1533)
                     /locus_tag="VNQ33_09965"
     CDS             complement(673..1533)
                     /locus_tag="VNQ33_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98473.1"
                     /translation="MVATGRWEAVRDGVYRIGAGPWTWDEQPMAAVLAAGDDAYAAFR
                     TAARLHEWVDRTGRLELLTDGYRRVRLPGVLAHRTIHLLPEDVTTVRGIPTTSPTRTL
                     IDLGARQPEATLGRWVDLGIQRGIVDLADLARRTSELNIRGRPKPIPLMRALRLRSGD
                     HDPGRSALEARVIEAVARRGLPELVRQHPVIRPDGRTAFIDLARPSAMVAVELDGWET
                     HGIRSAFEPDRIRANELLLLGWHLLRFTWHMSNDYICDTIDTAIQLHEPRLISGHGGT
                     LWVPRRPEIA"
     gene            complement(1862..>2664)
                     /locus_tag="VNQ33_09970"
     CDS             complement(1862..>2664)
                     /locus_tag="VNQ33_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ98474.1"
                     /translation="DPLKDDPGAVQLLFTAFNRWLEEDWGFAYQDRIFAAPYITLVDV
                     DWACAELEWAIERGARTIVTRPAAPTTADGPFSPGNVRFDPFWARVNEAGITVVVHAG
                     DSGYTSHGYATDGFTTDFGGVPQPIKMLQLERPIEDWLSSLICDNLFHRFPNLRVASV
                     ENGSKFLPGMFERLQILGRKMNGWFPEDPVETFRRHVWVNPFWEDQVDDVIAACGAER
                     VIFGSDWPHIEALPHPLDYVAEVQHLPEATQKLILADNVATLNHPHPA"
BASE COUNT          362 a          883 c          975 g          444 t
ORIGIN      
        1 tgccgctgtt cggcgcgatc gtcgcctacg tgctgggcga ccaccacgcg ctgacccgca
       61 gtcgcatcct cgggctggtc atcggcgtcg cgggcgtcgc cctcgtggtc ggcgcggctg
      121 gcggcgacgg cgacgtccgc ctggtcaacg tggccgaggt cctggtggtg gccgtctgct
      181 acgccgtcgc cccgttcatc acggctcgca agctcaccga ggtgccgggc gtcggcctcg
      241 ccgccctcgc cctcgccatg gtggcggtgg gctacatcgt ccccgcctcg ctcacccagg
      301 accagacgcc caccggtcgc agcatcggtg cgctcgtcgc cctggcggtc ctctgcacgg
      361 cgatcgcctt cgtcatcttc ttcgccctca tccgcgaggt cggcccggct cgcgccacgg
      421 tgatcacgtt catcaacccg atcgtggccc tggcgctcgg cgtggtcgtc ctcgacgagc
      481 acctctcgtg gggccagatc ctcggcctgc ccatcgtgtt ggccggttgc tggctcgccg
      541 ccggccggcg tcccgccgag gtcgaacccg ccgccgagct cgcgaccccc gcccccgccg
      601 actgaccgat ttcgggtcac ctgtgcaccc catcggtgct cacacgacct gaaatgccgc
      661 agccggtccg gattacgcga tttcgggtcg tctgggcacc cacagggtgc cgccgtgacc
      721 cgaaatcagg cggggctcgt ggagctggat cgccgtgtcg atcgtgtcgc agatgtagtc
      781 gttgctcatg tgccaggtga accggaggag gtgccagccg aggaggagca gctcgttggc
      841 ccggatgcgg tcgggttcga aggcagatcg gatgccgtgg gtctcccatc cgtcgagctc
      901 gaccgcgacc atcgctgatg gccgagcgag gtcgatgaac gcggtgcgac catcgggccg
      961 gatgacgggg tgctggcgca ccagctcggg aagacctcgt cgggcgacgg cctcgatgac
     1021 gcgcgcctcg agcgcggacc gccctgggtc gtgatctccc gagcggaggc gaagcgctcg
     1081 catgagcgga atcggcttcg gtcggccacg gatgttcagc tcgctcgtgc ggcgggcgag
     1141 atctgcgagg tcgacgatgc cgcgctggat ccccaggtcg acccagcggc ccaacgtggc
     1201 ttcgggctgc cgtgcgccga gatcgatcag ggtccgagtc ggggaggtgg tcgggatgcc
     1261 ccggacggtc gtcacgtctt cgggaagcag gtggatcgtc cgatgagcca agacgccagg
     1321 gagcctcacg cggcggtagc cgtcggtcag cagttcgagc cggccggttc ggtcgaccca
     1381 ctcgtgcaat cgggcggccg ttcggaacgc tgcgtaggcg tcatcgccgg cagcgagcac
     1441 ggcggccatc ggctgttcgt cccacgtcca tggtccggcg ccgatccggt agacgccgtc
     1501 gcgcaccgcc tcccatcgcc cggtcgccac catcgtctgg atcgagcgtc gcgtgccgcc
     1561 gaactcgact gcttgacggc gtgagatcaa tccgtgctgg ccgcgcgcga tgcgccggat
     1621 cgctcgatgg tccatgtgtg cctccgctcg cgtcgggtcc gaccggggaa ggcggatgga
     1681 cgatgacgga cgcgccggcc gtcgtcacgc gatgcgctgt atctggagca tcgatgtttc
     1741 gggtcgcctg tgcaccgtcg gggtgcccag acgacccgaa atcggctggt ggaccagttc
     1801 ggcgggattt ggggtcgcct gtgcaccggt ggggtgctca ggtgacccga aatcgtctgg
     1861 gttaggcggg gtgggggtgg ttgagggtgg cgacgttgtc ggcgaggatc agcttctggg
     1921 tggcctcggg gaggtgctgc acctcggcga cgtagtcgag ggggtggggg agggcctcga
     1981 tgtggggcca gtcgctgccg aagatcaccc gctcggcacc gcaggcggcg atgacgtcgt
     2041 cgacctggtc ttcccagaac gggttgaccc agacgtggcg gcggaacgtc tccaccgggt
     2101 cctcggggaa ccagccgttc atcttgcggc cgaggatctg cagccgctcg aacatcccgg
     2161 ggaggaactt cgagccgttc tcgaccgagg cgacgcggag gttcgggaac cggtggaaga
     2221 ggttgtcgca gatgagcgac gacagccagt cctcgatggg ccgctcgagc tggagcatct
     2281 tgatcggctg cggcaccccg ccgaagtcgg tggtgaaccc gtcggtggcg tagccgtgcg
     2341 acgtgtagcc ggagtcgccc gcatggacca ccacggtgat gccggcctcg ttgacccgcg
     2401 cccagaacgg gtcgaagcgc acgttgcccg gcgagaacgg accgtcggcg gtggtggggg
     2461 cggccgggcg ggtgacgatg gtgcgggccc cgcgctcgat ggcccactcc agctcggcgc
     2521 aggcccagtc gacgtcgacg agcgtgatgt acggcgccgc gaagatgcgg tcctggtagg
     2581 cgaagcccca gtcctcctcg agccaccggt tgaacgcggt gaagagcagc tggacggcac
     2641 ccgggtcgtc cttcagcggg tcct
//
LOCUS       DATMFS010000530         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_157869, whole genome shotgun sequence.
ACCESSION   DATMFS010000530 DATMFS010000000
VERSION     DATMFS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157869"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..755)
                     /locus_tag="VNQ33_09975"
     CDS             complement(78..755)
                     /locus_tag="VNQ33_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98475.1"
                     /translation="MTTLLPPSCARCGAPLPAVAADPTHPEWCGACAARALDAPGMPP
                     SVPSTSGRTSQAPWNNPATAAPPADGGLPVVVTRPLDGALTGLAAATLGGVLWWAASV
                     AVFTSQPAFEWWPLGSVLVGLVTGIGVLLGTRRGGLVSGLLALVFSSAAVLAAVYFID
                     RSHAIIALEDAGRPADIPLWQGFRHVADVYRGWWDLDREHALMWLAGPVVAVLIAGWP
                     NRRPIGA"
     gene            1005..1985
                     /locus_tag="VNQ33_09980"
     CDS             1005..1985
                     /locus_tag="VNQ33_09980"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWJ98476.1"
                     /translation="MAHRIAVIGGDGIGPEVVAEALKVVRAAGVALDTTDFDLGGARY
                     LRDGTVLPDDVLDELRGFDAILLGAVGTPDVPPGVIERGLLLKMRFALDQYVNLRPFA
                     LPEKDIDFFVVRENTEGTYAGEGGFLRKGTPHEIATQGSVNTRLGVERVVRYAFELAQ
                     SRPRKHLTMVHKTNVLTFSGDLWQRVFDLVAAEYPEVTTAYNHVDAACIYFVQDPQRY
                     DVIVTDNLFGDILTDLGGAVSGGIGFASSGNLDPSRQAPSMFEPVHGSAPDIAGQAKA
                     NPIAAILSAAMMVELLGEAGAAERIRKACAAHTDLDASTPEIGDAIAASL"
BASE COUNT          322 a          784 c          702 g          272 t
ORIGIN      
        1 cgtcttgtga cagcgggatc ggctcgtcgc tcatgggcgg cagtctagga gcgtgggccg
       61 agacgccctt ccggcgatca ggcgccgatg ggccggcggt tcggccagcc ggcgatcagc
      121 acagccacca ccggcccggc cagccacatg agagcgtgct cccgatcgag gtcccaccag
      181 ccccggtaca cgtcggcgac gtggcggaac ccctgccaca gcgggatgtc cgccggacgc
      241 cccgcatctt cgagggcgat gatcgcgtgg ctgcgatcga tgaagtagac ggcggcgagg
      301 acggcggcgg agctgaacac gagcgcgagc agtcccgaga cgagaccgcc gcgccgggtg
      361 cccagcagga ccccgatccc ggtgacgaga ccgacgagca ccgaaccgag gggccaccac
      421 tcgaaggccg gctggctcgt gaagacggcg acggaagcgg cccaccacag cacgccgccg
      481 agggtcgccg ccgccagccc cgtcaaggcg ccgtcgagcg gacgggtgac gaccaccggc
      541 aacccgccgt cggcgggagg cgcggccgtg gcggggttgt tccagggcgc ctggctggtg
      601 cgcccggacg tgctcgggac cgacggcggc atcccgggcg cgtcgagggc ccgagctgca
      661 caggcgccgc accactcggg gtgggtggga tcggcggcga cggccggcag gggcgctccg
      721 cagcgggcac aggacggggg cagcaacgtg gtcacctcac gaggacggtc cggctgaccg
      781 tactccggtc gccgctcctt gcggggaaac ggcaagacgg gcctacactt gaccgcatgt
      841 cgcgtccccg cctggtcatc accatcgggt agcgcacacc gatccatcgg tgtgcgcctt
      901 gagccgtcca cttcggtggg cggctttgtc gttgtcagcg catctgcgcc ggccgtgagc
      961 gcgtcacgag cacgaacgaa gaacgagcag agacggagca cctggtggca catcggatcg
     1021 cagtgatcgg cggcgacggg atcggaccgg aggtcgtggc cgaggccctg aaggtcgtcc
     1081 gcgctgcggg cgtggcgctc gacaccaccg acttcgatct cggcggcgcc cgctacctgc
     1141 gcgacggcac cgtgctgccc gacgacgtcc tcgacgagct gcggggcttc gatgccatcc
     1201 tcctcggcgc ggtcggcacc cccgacgtgc cacccggggt catcgagcgc ggactgctgc
     1261 tcaagatgcg cttcgcgctc gaccagtacg tgaacctgcg gccgttcgcc ctgccggaga
     1321 aggacatcga cttcttcgtg gtccgggaga acaccgaggg cacctatgcc ggcgagggcg
     1381 gcttcctccg caagggcacg ccccacgaga tcgccaccca gggctcggtc aacacccgcc
     1441 tcggcgtcga gcgggtcgtc cgctacgcct tcgagctggc ccagagccgg ccccgcaagc
     1501 acctcaccat ggtccacaag accaacgtgc tcacgttctc cggcgacctg tggcagcggg
     1561 tgttcgacct cgtcgccgcc gagtaccccg aggtcaccac ggcgtacaac cacgtcgacg
     1621 ccgcctgcat ctacttcgtg caggacccgc agcgctacga cgtcatcgtc accgacaacc
     1681 tcttcggcga catcctcacc gacctcggcg gtgccgtgtc cggcgggatc ggcttcgcca
     1741 gctccggcaa cctcgacccg tcgcgccagg cgccctcgat gttcgagccg gtccacggct
     1801 ccgcgcccga catcgccggc caggccaagg ccaatcccat cgccgccatc ctctcggcgg
     1861 cgatgatggt ggagctgctc ggcgaggccg gcgcggccga gcgcatccgc aaggcgtgcg
     1921 ccgcccacac cgacctcgac gcctccaccc ccgagatcgg cgacgccatc gccgcctcgt
     1981 tgtagatccg ctctcgaccc ctcctcccac ccgcccacct gtccacgctg ctcgtcctcc
     2041 gaccgatcca gcacccgccg ggctcgcccg cctgacgcac
//
LOCUS       DATMFS010000531         2497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_88261, whole genome shotgun sequence.
ACCESSION   DATMFS010000531 DATMFS010000000
VERSION     DATMFS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88261"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..845
                     /locus_tag="VNQ33_09985"
     CDS             57..845
                     /locus_tag="VNQ33_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ98477.1"
                     /translation="MPSENVVASEPTDRRARKREARRDHLLDLALDLVDEHGVDGLTM
                     AALAEAGDYATASLYTYFASRSALLAALQERALVVLGRVAEEGAANWDRALAADADAT
                     GEVAALARLCAFSDLFLAAPTHHRREFRLQQQLLVTPGLEEAADANTVLPVALHVLGV
                     PQRLLAEAVEVGALRPGGPVEDPIGGAVDASFARTLAWVVALNGALLTDGLVTGLPTT
                     GALLGSQLTDALLAGWGADPADLAAARTRSARWQPAGAVSRTTR"
     gene            842..1477
                     /locus_tag="VNQ33_09990"
     CDS             842..1477
                     /locus_tag="VNQ33_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWJ98478.1"
                     /translation="MITTALTLAAAFAGGFLLWILFEYLLHRFAMHELHGKGIMSREH
                     LNHHVAAGWAFSYIHLLSWAGMLLVGFVLWMPLGWVTVSLAFGITVAVGWTAGYFFYE
                     YQHASAHLRGPRNQYEAWVRKHHFHHHFGHPMANHGVTLGIWDKVFGTLEEPERVRVP
                     RRMAQPWMLDEDGELKDEYTADYLLVGSRDTNERLAQLDKARAFASIAPTD"
     gene            1611..>2497
                     /locus_tag="VNQ33_09995"
     CDS             1611..>2497
                     /locus_tag="VNQ33_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ98479.1"
                     /translation="MAETTAPGRRIAGAPGEFQLAFEQAPIGIALVAPDGQFLWVNEA
                     LTRICGYDHETLLRLTFQDITHPDDLDADLALLESVIAGEITAYSMEKRYFHARGHEV
                     PIQLDVSMVRSADGSPRYFISHVQDITERRATVEALASAHDALRASQQRFAALVERST
                     DIICIIDEQGCIAYHSPAAERLLGYAPGSWLGTPFTAVVHPDDQAALNETFRRLVLDP
                     TLMPLAEVRVTTAEGEWRHVEISATNRLADPAVRGIVANVRDVTERAEAATRLAWQAF
                     HDSLTGLPNRALLADRLGHA"
BASE COUNT          341 a          938 c          845 g          373 t
ORIGIN      
        1 tgggtttggg gtgggggggg ttgaccgagg ttgacgatgt gggtacgttt ttcgatgtgc
       61 cttcggaaaa cgtcgttgcc tcggaaccca ccgatcggcg ggcccgcaag cgcgaggcgc
      121 gtcgggacca cctgctggac ctggcgctgg acctggtcga cgagcacggc gtcgacgggt
      181 tgacgatggc ggcgctggcg gaggcgggcg actacgccac ggcgtcgctg tacacctact
      241 tcgcctcgcg cagcgccctg ctcgccgcgc tccaggagcg ggccctcgtc gtgctcggcc
      301 gggtcgcgga ggagggcgcg gccaactggg accgcgccct cgctgccgat gccgatgcca
      361 ccggcgaggt ggcggcgctg gcgcgcctgt gcgcgttctc cgacctgttc ctcgccgcgc
      421 cgacgcacca ccggcgcgag ttccggctgc aacagcagct gctggtcacc cccggcctgg
      481 aggaggcggc cgatgccaac accgtgctgc ccgtcgccct ccacgtgctc ggcgtgcccc
      541 agcgcctgct cgccgaggcc gtcgaggtcg gcgccctgcg cccgggtgga ccggtcgagg
      601 accccatcgg cggtgccgtc gatgcctcct tcgcccgcac cctcgcctgg gtggtggccc
      661 tcaacggcgc cctgctcacc gatgggttgg tcaccggcct gcccaccacc ggggcgctgc
      721 tcggcagcca gctcaccgat gcactgctcg ccggctgggg tgccgatccc gccgacctcg
      781 ctgctgcccg cacccgttct gcccgctggc agcccgccgg cgccgtctcg aggaccaccc
      841 gatgatcacc accgccttga ccctcgccgc cgcgttcgcc ggcgggttcc tcctctggat
      901 cctcttcgag tacctgctcc accggttcgc catgcacgag ctgcacggca aggggatcat
      961 gagccgcgag cacctcaacc accacgtcgc ggcaggctgg gcgttctcgt acatccacct
     1021 cctcagctgg gcggggatgc tgctcgtcgg gttcgtcctg tggatgcccc tcggctgggt
     1081 cacggtgagc ctggcgttcg gcatcaccgt ggcggtcggt tggacggcgg gctacttctt
     1141 ctacgagtac cagcacgcct cggcccacct ccgcggtccc cgcaaccagt acgaggcctg
     1201 ggtccgcaag caccacttcc accaccactt cgggcacccg atggccaacc acggcgtcac
     1261 cctcggcatc tgggacaagg tgttcgggac gctcgaggag cccgagcggg tccgggtgcc
     1321 gcgccggatg gcccagccgt ggatgctcga cgaggacggc gagctcaaag acgagtacac
     1381 cgccgactac ctcctcgtgg ggtcgcgcga cacgaacgag cgcctggccc agctggacaa
     1441 ggcccgggcc ttcgcgtcca tcgcaccgac cgattgatcc ccgcttgacc tgtcgctgac
     1501 ggacctgtcg gcgagtcgcc cgatccggcc ctacgctggc gatcgctaca ggagcgtggt
     1561 gcgccgaccg atgagagctg gtcggaaccg ggacgagggg atcggggtgc atggctgaga
     1621 cgacggcgcc cgggcgacgc atcgccggcg cccccggcga gttccagctc gcgttcgagc
     1681 aggcacccat cgggatcgcg ctcgtggcgc cggacggcca gttcctgtgg gtcaacgagg
     1741 cgctgacgcg gatctgcggc tacgaccacg agaccctgct gcgcctcacg ttccaagaca
     1801 tcacgcaccc cgacgacctc gacgccgacc tggcgctgct ggagtcggtg atcgccggcg
     1861 agatcacggc gtactccatg gagaagcgct acttccacgc tcggggccac gaggtgccga
     1921 tccagctcga cgtctcgatg gtgcggtcag ccgacggatc cccgaggtac ttcatctccc
     1981 acgtgcagga catcaccgag cgccgggcca cggtcgaggc cctggcctcg gcccacgacg
     2041 cgctccgggc cagccagcag cggttcgccg cgctcgtcga gcggagcacc gacatcatct
     2101 gcatcatcga cgagcagggg tgcatcgcct accacagccc cgcagcggag cggctgctcg
     2161 gctacgcgcc gggctcgtgg ctcggcacgc ccttcaccgc ggtggtgcac cccgacgacc
     2221 aggccgcgct caacgagacc ttccgccgcc tcgtgctgga cccgaccctc atgccgctcg
     2281 ccgaggtgcg ggtgaccacg gccgagggcg agtggcgcca cgtcgagatc tccgccacca
     2341 accgcctcgc cgatccggcc gttcggggca tcgtcgccaa cgtccgcgac gtgaccgagc
     2401 gggccgaggc ggccacccgc ctggcgtggc aggcgttcca cgactcgctg accggcctgc
     2461 cgaaccgcgc gctgctcgcc gatcgcctgg gccacgc
//
LOCUS       DATMFS010000532         3929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_178654, whole genome shotgun sequence.
ACCESSION   DATMFS010000532 DATMFS010000000
VERSION     DATMFS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178654"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="pdxT"
                     /locus_tag="VNQ33_10000"
     CDS             complement(<1..455)
                     /gene="pdxT"
                     /locus_tag="VNQ33_10000"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWJ98480.1"
                     /translation="MKVGLLALQGAIGLHGDAIAALGATPVEVRTPDQLAGVDALIIP
                     GGESTTMSKLLDTSGLASPLRDRLAAGMPAFGTCAGMIVLARTVLDGRADQSSYAAID
                     VTVRRNAFGRQVDSFEADLAVDGLDAPFRAVFIRAPFVEEVGDGVEVLAS"
     gene            complement(458..1384)
                     /gene="pdxS"
                     /locus_tag="VNQ33_10005"
     CDS             complement(458..1384)
                     /gene="pdxS"
                     /locus_tag="VNQ33_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWJ98481.1"
                     /translation="MSESPTSLPNTVSSSTTPATGTQLVKRGLAEMLKGGVIMDVVDP
                     DQAKIAEDAGAVAVMALERVPSDIRRDGGVARMSDPEMIEGIQAVCTIPVMAKARIGH
                     FVEAQILEALGVDYIDESEVLTPADEAHHIDKWAFTVPFVCGATNLGEALRRISEGAC
                     MIRSKGEAGTGNVVEAVRHLRSILGDIRKITQADGPELFDWAKQLQAPLPLVQEIHDT
                     GKLPVPLFCAGGLATPADAALAMQLGAESVFVGSGIFKSDNPSLRAKAIVEATTHYND
                     PAILAKVSRGLGGAIPGLEISKLETTYADRGW"
     gene            complement(1438..2565)
                     /locus_tag="VNQ33_10010"
     CDS             complement(1438..2565)
                     /locus_tag="VNQ33_10010"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWJ98482.1"
                     /translation="MSRIAELWAGLSTNARVGVIGGAVGVVALGGVAAVALSGGGSND
                     PAPTTTTEAATTAAPTTTSTTAVPTGPTAPLTGLAVQEEDAPLLFRTALAVKIDNLDA
                     PGESAVPQTAIPKADVVFEEIVEGNITRLVAVFHSQQPGTVGPVRSARTTDVQLLPQL
                     GHPLLAWSGGNDGAVAAVRSSPFLIDAGADAAPGSYFRDRSRRAPHNLYVHADELFGR
                     APQGTPGPQALFLYRKAGEGNPPAAQGAQGADLNWGGGGASANVSWRWDASERVYKRE
                     QRGRPHLDSEGAQLTAANVVVLATDYGQSAADSRSPEAQTVGSGELFVFTDGAVIHGR
                     WDRPDIAKPATLVDDTGAPVLLTPGRTWVELPRPGGTAVVR"
     gene            complement(2628..3536)
                     /locus_tag="VNQ33_10015"
     CDS             complement(2628..3536)
                     /locus_tag="VNQ33_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWJ98483.1"
                     /translation="MYDQVAQNKRRSAAIVTGFVLFTTLVIAVILWLFDLGFVGIGIA
                     AVVAIASAWGAYWKSDQIALKMSRAVPADEHTYARLHNLVEGLCIASGIPKPRIYIVD
                     DPAPNAFATGRNPKHAAVAVTTGLLDMMTRVELEGVLAHELSHIKNDDILVSTLAVTM
                     VGMVTLAADISVRMMWWNGGRNGRSSDREGGAAPFLAIFGLALLVLSPLLAKLMQMSV
                     SRSRESLADMSAIEMTRYPPGLISALEKLRDDTTVVHASSRATAHLWIEAPVARDEEE
                     GKLSKWNKLFDTHPPIEERIAALQEL"
     gene            complement(3544..>3929)
                     /locus_tag="VNQ33_10020"
     CDS             complement(3544..>3929)
                     /locus_tag="VNQ33_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWJ98484.1"
                     /translation="VKGYAAHERETLDAVIQARNMAQQASGPAEAAAADNVLTGALKS
                     VFALSEAYPDLKANQNFLALQEELTGTEDKIAYSRQFYNDTVNRYNTKIQSLPSNIIA
                     GMFHFTEREYFEAAGEARGPVNVQF"
BASE COUNT          536 a         1298 c         1506 g          589 t
ORIGIN      
        1 gaggccagga cctcgacgcc gtcgcccacc tcctcgacga acggggctcg gatgaacacg
       61 gcgcggaacg gcgcgtccag gccgtcgacg gcgaggtcgg cctcgaacga gtcgacctga
      121 cggccgaacg cgttgcgccg caccgtcacg tcgatggcgg cgtaggagct ctggtcggcc
      181 cggccgtcga gcacggtgcg ggccaagacg atcatccccg cgcaggtgcc gaacgcaggc
      241 atgcccgcgg cgaggcggtc gcgcagcggc gaggccaggc cggacgtgtc gagcagcttg
      301 gacatcgtgg tggactcgcc gccggggatg atcagcgcat cgacgccggc cagctggtcc
      361 ggcgtgcgga cctcgaccgg cgtggcgccg agggcggcga tggcatcgcc gtgcaggccg
      421 atcgcgccct ggagggcgag caggccgacc ttcacggcta ccagccgcgg tcggcgtagg
      481 tggtctcgag cttggagatc tcgagaccgg ggatcgcacc gccgaggccg cggctgacct
      541 tggcgaggat ggccgggtcg ttgtagtgcg tggtggcctc gacgatcgcc ttggcgcgca
      601 gcgaggggtt gtcggacttg aagatgcccg acccgacgaa cacggactcg gcgccgagct
      661 gcatggcgag ggcggcgtcg gccggggtgg ccaggccacc ggcacagaac agcgggacgg
      721 ggagcttgcc ggtgtcgtgg atctcctgga cgagcgggag cggggcctgg agctgcttgg
      781 cccagtcgaa gagctcgggg ccgtcggcct gggtgatctt gcggatgtcg ccgaggatgg
      841 agcgcaggtg gcgcacggcc tcgaccacgt tgccggtgcc ggcctcgccc ttggagcgga
      901 tcatgcacgc accctcggag atgcggcgca gggcctcgcc caggttggtg gcgccgcaca
      961 cgaacggcac ggtgaaggcc cacttgtcga tgtggtgggc ctcgtcggcc ggggtgagga
     1021 cctcggactc gtcgatgtag tcgacgccga gcgcctcgag gatctgcgcc tccacgaagt
     1081 ggccgatgcg ggccttggcc atcaccggga tggtgcagac ggcctggatg ccctcgatca
     1141 tctccgggtc ggacatgcgg gccaccccgc cgtcgcggcg gatgtcagac gggacccgct
     1201 cgagcgccat gacggcgacg gcgccggcgt cctcggcgat cttggcctgg tcggggtcga
     1261 ccacgtccat gatcacgccg cccttgagca tctcggcgag gcctcgcttg accagctggg
     1321 tgccggtggc gggggtggtg ctggacgaga cggtgttcgg cagggacgtg ggcgactcgg
     1381 acatgccgtc agtctagaga cggacccccg cgatcccgaa gcccgttgcg gagcggatca
     1441 cctcaccacc gccgtgccac cgggccgggg cagttcgacc cacgtacggc ctggggtgag
     1501 cagcaccggg gcaccggtgt cgtcgaccag ggtggccggc ttggcgatgt cgggccggtc
     1561 ccaccgcccg tggatcacgg caccgtcggt gaacacgaac agctcgcccg atccgacggt
     1621 ctgggcttcg ggcgaccggg agtccgccgc gctctggccg tagtcggtgg ccagcacgac
     1681 gacgttggcg gccgtgagct gcgcgccctc ggaatccagg tgcgggcgcc cacgctgctc
     1741 ccgcttgtag acgcgctcgc tggcgtccca ccgccagctc acgttggcgc tggcgccacc
     1801 gccgccccag ttcaagtcgg cgccctgcgc gccctgggcg gcgggcgggt tgccctcgcc
     1861 ggccttgcgg tagaggaaca gcgcttgggg gcccggcgtc ccctgcgggg cccgcccgaa
     1921 cagctcgtcg gcgtgcacgt agaggttgtg cggcgcccgg cggctgcggt cgcggaagta
     1981 ggagcccggc gcggcgtcgg cgccggcatc gatcaggaac gggctgctgc gcaccgcggc
     2041 gaccgcgccg tcgttgccgc ccgaccaggc caggagcggg tggccgagct gcgggagcag
     2101 ctgcacgtcg gtggtgcggg ccgagcgcac gggaccgacc gtccccggct gctgggagtg
     2161 gaacacggcc acgaggcggg tgatgttgcc ctcgacgatc tcctcgaaca cgacgtcggc
     2221 cttggggatc gccgtctgcg ggacggcgct ctcgcccggc gcgtcgaggt tgtcgatctt
     2281 gacggccagc gccgtgcgga acagcagcgg cgcgtcctcc tcctgcacgg ccaggccggt
     2341 cagcggcgcg gtcgggccgg tggggacggc ggtggtcgag gtcgtcgtcg gtgccgcggt
     2401 ggtggccgcc tcggtcgtgg tggtgggcgc agggtcgttc gagccaccgc cgctgagcgc
     2461 gacggccgcc acgccaccga gggcgacgac gccgacggcg cccccgatga cgcccacgcg
     2521 ggcgttcgtg ctgagaccgg cccagagctc ggcgatgcgg gacatgggga aagcgttctc
     2581 ctcgcgccgc cgtgtggggt ggatggtggg tcctgccagg accggagcta cagctcctgc
     2641 agcgcggcga tgcgctcctc gatgggtggg tgcgtgtcga agagcttgtt ccacttcgag
     2701 agcttcccct cctcctcgtc acgggcgacc ggcgcctcga tccagaggtg ggccgtggcc
     2761 cgcgacgagg cgtggacgac ggtggtgtcg tcgcgcagct tctcgagcgc cgagatcagc
     2821 cccggcgggt agcgcgtcat ctcgatggcc gacatgtcgg cgagcgactc ccgcgagcgg
     2881 ctcaccgaca tctgcatcag cttggcgagc agcggcgaca agacgaggag cgccagcccg
     2941 aagatggcga ggaacggggc ggcgccgccc tcgcggtcgc tgctgcggcc gttgcgcccg
     3001 ccgttccacc acatcatccg cacgctgatg tcggcggcga gggtgaccat gcccaccatg
     3061 gtgacggcca gggtcgacac gaggatgtcg tcgttcttga tgtggctcag ctcgtgggcc
     3121 aagacgcctt cgagctcgac ccgggtcatc atgtcgagca gcccggtggt gacggcgacc
     3181 gccgcgtgct tcgggttgcg acccgtggcg aaggcgttcg gcgccggatc gtcgacgatg
     3241 tagatccgcg gcttcgggat cccgctggcg atgcagaggc cctccaccag gttgtggagg
     3301 cgggcgtagg tgtgctcgtc ggcgggcacc gcccggctca tcttcagggc gatctggtcc
     3361 gacttccagt aggcccccca cgccgacgcg atggcgacga ccgcggcgat gccgatgccg
     3421 acgaacccga ggtcgaagag ccagaggatg acggcgatga cgagggtcgt gaagaggacg
     3481 aacccggtga cgatcgccgc ggaccggcgt ttgttctgag cgacttggtc gtacacgcgg
     3541 gggtcagaac tgcacgttca cggggccacg ggcctcgccc gccgcctcga agtactcgcg
     3601 ctcggtgaag tggaacatgc cggcgatgat gttcgacggg agcgactgga tcttggtgtt
     3661 gtacctgttc acggtgtcgt tgtagaactg ccgcgagtag gcgatcttgt cctcggtgcc
     3721 ggtgagctcc tcctgcagcg ccaggaagtt ctggttcgcc ttgaggtccg ggtaggcctc
     3781 cgacagggcg aacaccgact tcagcgcgcc ggtgaggacg ttgtcggcgg cggcggcctc
     3841 cgccggcccc gatgcctgct gggccatgtt gcgggcctgg atcaccgcgt cgagcgtctc
     3901 gcgctcgtgg gcggcgtagc ccttcacgg
//
LOCUS       DATMFS010000533         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_204127, whole genome shotgun sequence.
ACCESSION   DATMFS010000533 DATMFS010000000
VERSION     DATMFS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204127"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="sufD"
                     /locus_tag="VNQ33_10025"
     CDS             <1..1132
                     /gene="sufD"
                     /locus_tag="VNQ33_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWJ98485.1"
                     /translation="AAAAEAFGAAAFPSAQEEVWRYSRIAELDLDRYAPSADAVAVDL
                     PIPEEWTAKGVAVRRAADLVDETSGIGSVAQGGPDVFATLNDAFAADPVVVSVPRGAQ
                     LTEPLVVGTRLAGAGAAGFPRLVVLVGEGAAATVIERTTSTDDDLLSVPLTELRVERG
                     GRLDFVAIQQLGSAAWQLGSLVAEVGQEAHLRVGLVGLGGAYARLRTDCRLTGRGGTG
                     DLLAAYFGDGDQTLDFRTFQHHDAPDTTSNLLFKGAVGGRSRSVYTGLIKVSKDARGT
                     NAFQTNRNVKLSDDAWAESVPNLEIETNDVRCSHASTVGPVDPEQRFYLESRGVPPTV
                     ADRLIVAGFFDEVVRELPDDALVAEVRSRISEKLDRVAEDLR"
     gene            1129..1473
                     /locus_tag="VNQ33_10030"
     CDS             1129..1473
                     /locus_tag="VNQ33_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWJ98486.1"
                     /translation="MSTGTHPIAPLDALADGEAQRFEVQGHRLAVVRIGDDVYVIGDI
                     CTHQDISLSEGEVDAETCHIECWKHGSSFSLETGEPDVLPATRPVPVYRSWVDGGEVY
                     VEIDDAEGTAHV"
     gene            1466..>2125
                     /gene="sufC"
                     /locus_tag="VNQ33_10035"
     CDS             1466..>2125
                     /gene="sufC"
                     /locus_tag="VNQ33_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWJ98487.1"
                     /translation="MSELVIEGLRAGVDGKEILQGIDLVVRSGEVHAVMGPNGSGKST
                     LSHVILGRPGYEVTGGSVTLDGVDVLAMDTWERAQAGLFLALQYPTEVPGVSVESVLT
                     AAFAAGGRSTEGLHATMAAEAERIGFAERFLGRDLNVDLSGGEKKRNETLQLGVIEPK
                     IAILDELDSGLDVDALRACSRRVEQATTETGLGVLAITHYTRLLDELKPDVVHILAKG
                     RI"
BASE COUNT          296 a          778 c          750 g          301 t
ORIGIN      
        1 ggccgctgcg gccgaggcgt tcggggcagc ggcgttcccg agcgcgcagg aggaggtctg
       61 gcgctacagc cggatcgcgg agctggacct cgaccggtac gcgccgtcgg ccgacgcggt
      121 ggcggtggac ctgccgatcc ccgaggagtg gacggcgaag ggcgtcgcgg tccggcgcgc
      181 cgcggacctc gtcgacgaga ccagcggcat cggttcggta gcccagggcg gccccgacgt
      241 cttcgccacc ttgaacgacg ccttcgccgc cgacccggtc gtcgtgtcgg tgccccgcgg
      301 cgcgcagctc accgagccgc tcgtcgtcgg cacccgcctc gctggcgcgg gcgccgcggg
      361 cttcccccgc ctcgtcgtgc tcgtggggga aggtgcggcc gccaccgtca tcgaacgcac
      421 cacctccacc gacgacgacc tgctgtcggt cccgctcacc gagctgcggg tcgagcgcgg
      481 cggccggctg gacttcgtgg ccatccagca gctcggctcg gcggcctggc agctgggctc
      541 gctcgtggcc gaggtcggtc aggaggcgca cctgcgcgtc ggcctcgtcg gcctgggcgg
      601 cgcctacgcc cgtctccgca ccgactgccg tctcaccggc cgcggcggca ccggcgacct
      661 gctcgcggcg tacttcggcg acggcgacca gacgctcgac ttccgcacgt tccagcacca
      721 cgatgcgccc gacaccacgt cgaacctcct gttcaagggg gccgtcggcg ggcggagccg
      781 gtcggtgtac acgggcctca tcaaggtcag caaggacgca cggggcacca acgccttcca
      841 gaccaaccgc aacgtcaagc tgtccgacga tgcgtgggcc gagtcggtcc ccaacctgga
      901 gatcgagacc aacgacgtgc gctgcagcca cgcgtccacg gtcgggccgg tcgatccgga
      961 gcagcgcttc tacctggaga gccgcggcgt gccgccgacg gtggccgatc ggctgatcgt
     1021 cgccggcttc ttcgacgagg tcgtgcggga gctgcccgac gacgcgctgg tggccgaggt
     1081 ccgcagccgg atctccgaga agctcgaccg agtggccgag gaccttcgat gagcaccggc
     1141 acgcacccga tcgcgccgct cgatgccctc gccgacggcg aggcgcagcg cttcgaggtg
     1201 cagggccacc ggctggccgt cgtgcgcatc ggcgacgacg tctacgtcat tggcgacatc
     1261 tgcacgcacc aggacatctc cctgtccgag ggcgaggtcg acgccgagac ctgtcacatc
     1321 gagtgctgga agcacgggag ctcgttctcg ctggagacgg gcgagcccga cgtgctcccc
     1381 gccacccggc ccgtgcctgt gtaccgctcg tgggtcgacg gcggcgaggt ctacgtggag
     1441 atcgacgacg ccgaagggac cgctcatgtc tgagctcgtg atcgagggcc tgcgagccgg
     1501 cgtcgacggc aaggagatcc tccaggggat cgacctggtc gtccgctcgg gtgaggtcca
     1561 cgccgtgatg gggcccaacg ggtccggcaa gtccaccctc tcgcacgtca tcctcggccg
     1621 tcccggctac gaggtcacgg gcggctcggt caccctcgac ggcgtcgacg tgttggccat
     1681 ggacacgtgg gagcgggccc aggccggcct gttcctcgcc ctccagtacc cgaccgaggt
     1741 cccgggggtg tcggtcgagt cggtgctgac cgccgcgttc gccgccggtg gccgctccac
     1801 cgagggcctc cacgccacca tggccgccga ggccgagcgc atcggcttcg ccgagcggtt
     1861 cctcggccgc gacctcaacg tcgacctgtc cggtggtgag aagaagcgca acgagaccct
     1921 gcagctcggc gtgatcgagc ccaagatcgc catcctcgac gagctggact ccgggctcga
     1981 cgtcgatgcc ctgcgggctt gctcgcggcg ggtcgagcag gccaccaccg agaccgggct
     2041 cggcgtcctc gccatcaccc actacacccg gctgctcgac gagctgaagc ccgacgtggt
     2101 gcacatcttg gccaagggcc ggatc
//
LOCUS       DATMFS010000534         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_344440, whole genome shotgun sequence.
ACCESSION   DATMFS010000534 DATMFS010000000
VERSION     DATMFS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344440"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNQ33_10040"
     CDS             complement(<1..751)
                     /locus_tag="VNQ33_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98488.1"
                     /translation="MDDEGNDRAEGGPAAPPAPPWGLQQPPVFEQPPGLDAPPAFGPG
                     PAFGAPPPLPPPPVPPAWRRLIAPMGPVEGPRPAPTAASMVAVGGGLLAAAGLFALLS
                     ELDGYAQRTTGLAISLLFVALGVAISVVNRSSRAAAGGVALSIIGVVPFTVYVFANAD
                     LFDQFSGGEVDGNPWTGLRWTITLALITAAGLWLVGYLFVPTRRYGAFLGAALLAVWL
                     IPLCNLQLSAMEDMFRPFESVSTFEPVPSEPG"
     gene            876..>2319
                     /gene="metG"
                     /locus_tag="VNQ33_10045"
     CDS             876..>2319
                     /gene="metG"
                     /locus_tag="VNQ33_10045"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWJ98489.1"
                     /translation="MPGRYFLTTPIYYVNDVPHIGHAYTTVTADALARWHRLLGEEVF
                     FLTGTDEHGLKIARAAEANGITPQEQADRTSVRFQEAWDALDISYDRFIRTTDADHYA
                     AVQAFMEKVRENGHLYKETYAGWYCVACEAYYEESDLDPNPDGGPMLCRIHARPVEWF
                     EEDNWFFRLSAFEQPLLDWYEANPGAVSPAGKRNEAIGLIKQGLRDISISRTSIDWGV
                     PVPWDEGHVFYVWYDALINYATAVGYGADPERFDGWWPHVHHLLAKDILRFHCVYWPA
                     MLMAAGEAPPAHLNVHGYLLVGGEKMSKTRLNQIFPADLVEDFGVDGFRFHFLRYAPF
                     GPDGDVSYEGIVAGYNADLANNFGNLLSRVATVVAKKCGGTGPAPRPDSPLAAVAAEA
                     YRDAAAAWDAVQPSVALDATWRLIREANALLEQTEPWKADPGPEVDGVLGDALEVLRL
                     VCILASPALTRATDEAWRRIGLDGSPADQRL"
BASE COUNT          341 a          823 c          850 g          305 t
ORIGIN      
        1 agccgggttc gctgggcacc ggttcgaacg tgctgacgga ttcgaacggg cggaacatgt
       61 cctccatggc cgagagctgg aggttgcaca gcgggatgag ccagaccgcg aggagggcgg
      121 cgccgaggaa cgcgccgtag cggcgggtgg gcacgaacag gtagcccacg agccacaggc
      181 ccgcggcggt gatcagcgcc aaggtgatgg tccaccgcag gccggtccac gggttgccgt
      241 ccacctcgcc gccgctgaac tggtcgaaca ggtcggcgtt ggcgaacacg tagaccgtga
      301 aggggacgac gccgatgatc gacagggcaa cgccgccggc cgccgcccga gacgatcggt
      361 tcacgacgct gatggccacg ccgagcgcca cgaacaggag ggagatggcg agcccggtgg
      421 tgcgctgggc gtacccgtcg agctcgctca gcagggcgaa caggccggcc gcggcgagga
      481 ggccgccgcc gacggcgacc atcgaggcgg cggtgggcgc cgggcgcggt ccttcgaccg
      541 gccccatcgg ggcgatgagc cgccgccacg ccggagggac cggcggcggg ggcaggggcg
      601 gcggcgcccc gaaggcgggt cctggcccga aggccggcgg cgcatcgagc cccggcggct
      661 gctcgaacac cggcggttgc tggagccccc atggtggggc gggtggcgct gcgggtcccc
      721 cctcggctcg gtcgttgcct tcgtcgtcca tgcgcgccgc ctccctcgtg cccgatcggc
      781 cgtccgcagc gtagggcggc ggcggcctcg cgctgggcga ttcgcagggg tcatgtcccg
      841 gcggggtgcg cggggcgcct cgctagggtg atccggtgcc cggccgctac ttcttgacca
      901 cgccgatcta ctacgtgaac gacgtgcccc acatcgggca cgcgtacacg acggtgacgg
      961 ccgacgccct ggcccgctgg caccgcctgc tgggcgagga ggtgttcttc ctcaccggca
     1021 ccgacgagca cggcctcaag atcgcccgcg ccgccgaggc caacggcatc acgccccagg
     1081 agcaggcgga tcgcacgtcg gtccggttcc aggaggcgtg ggacgcgctc gacatctcct
     1141 acgaccgctt catccgcacc accgacgccg atcactacgc cgccgtgcag gccttcatgg
     1201 agaaggtgcg ggagaacggc cacctctaca aggagaccta cgccggctgg tactgcgtgg
     1261 cgtgcgaggc gtactacgag gagagcgacc tcgatccgaa ccccgacggc gggccgatgc
     1321 tctgtcggat ccacgcccgg ccggtggagt ggttcgagga ggacaactgg ttcttccggc
     1381 tctcggcgtt cgagcagccg ctcctcgact ggtacgaggc caacccgggc gccgtctccc
     1441 ccgcgggcaa gcgcaacgag gccatcggcc tcatcaagca ggggctccgg gacatctcga
     1501 tctcgcgcac gtcgatcgac tggggcgtgc cggtcccgtg ggacgagggc cacgtcttct
     1561 acgtctggta cgacgcgctc atcaactacg ccacggcggt gggctacggc gccgaccccg
     1621 agcggttcga cgggtggtgg ccccacgtgc accacctgct cgccaaggac atcctccggt
     1681 tccactgcgt gtactggccg gcgatgctga tggcggcggg cgaggcgccg cccgcccacc
     1741 tcaacgtcca cggctacctg ctcgtgggcg gggagaagat gagcaagacc cggctcaacc
     1801 agatcttccc ggccgacctg gtggaggact tcggggtcga cggcttccgg ttccacttcc
     1861 tccgctacgc cccgttcggt ccggacggcg acgtgagcta cgaggggatc gtggcggggt
     1921 acaacgcgga tctcgccaac aacttcggca acctgctcag ccgggtggcc accgtcgtcg
     1981 ccaagaagtg cgggggcacc ggcccggcgc cccgccccga cagcccgctc gccgcggtgg
     2041 cggccgaggc ctaccgggac gcggcggcgg cgtgggacgc ggtgcagccg tcggtcgcgc
     2101 tcgatgccac gtggcgcctg atccgggagg ccaacgccct gctggagcag accgagccgt
     2161 ggaaggccga cccggggccc gaggtcgacg gtgtgctcgg cgatgccctc gaagtgctgc
     2221 ggctcgtgtg catcctcgcc agccccgccc tgacccgggc caccgatgag gcctggcgcc
     2281 gcatcggcct cgacggctcg ccggccgacc agcgcctcc
//
LOCUS       DATMFS010000535         4889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_226977, whole genome shotgun sequence.
ACCESSION   DATMFS010000535 DATMFS010000000
VERSION     DATMFS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226977"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VNQ33_10050"
     CDS             <1..200
                     /locus_tag="VNQ33_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98490.1"
                     /translation="EGRADLFPAATSYSEPLRYGNGMWAKVSRAGLAAQLGYLVFAGL
                     IALIGVALVVLIVATLVAAAT"
     gene            249..662
                     /gene="ndk"
                     /locus_tag="VNQ33_10055"
     CDS             249..662
                     /gene="ndk"
                     /locus_tag="VNQ33_10055"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWJ98491.1"
                     /translation="MTNRTFIMCKPDAVERGLVGEVISRIERKGLTLAAVELRTADTA
                     LAEAHYAEHADKPFFGELVSFLTRSPVVAMIVEGPSDNTWELGRILIGKTQVTDAQPG
                     SIRGDFATTTNENLVHGSDGPESAEREIALWFPGR"
     gene            973..2016
                     /locus_tag="VNQ33_10060"
     CDS             973..2016
                     /locus_tag="VNQ33_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWJ98492.1"
                     /translation="MSADNPLSSIMGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAVN
                     VKDGRPLAVGLEAKRMIGRTPAHIQAIRPLKDGVIADFEICEKMLRYFIHRVHQRRWV
                     KPRMVICVPSGITGVEQRAVQEAAEYAGARKPAYIIEEPMAAAIGAGLPVQEPTGNMI
                     VDIGGGTTEVAVISLGGIVSSQSVRVGGDELDDAIIQYIKKEYSLALGERTAEEVKIA
                     LGSAWRLQEELHAEIRGRDLVTGLPKTIVTTTEEIREAIEEPVASICDAVKVTLDKTP
                     PELAADIMEQGIVLAGGGALLHGLDARLQHETGMPIVIAQNPLDCVAIGSGQSLEEFE
                     ALKGVLFSTNLAN"
     gene            2034..2879
                     /gene="mreC"
                     /locus_tag="VNQ33_10065"
     CDS             2034..2879
                     /gene="mreC"
                     /locus_tag="VNQ33_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWJ98493.1"
                     /translation="MAYSRRPSRTPRSRFTLALLLLTAVTLLVLDLPGTGPLDPVRNA
                     LGAAFRPFRAAGNAVFEPISNGWKGAFGYDDLRDENEQLKAQLEERKSEEADVKRLQQ
                     KVADLSKINDVRVEQQKTRVAEVVSGPLNSFEQTLEIDAGSSDGIKKGMAVITGRARG
                     KGGAVLGRISEVRSGSSTVQLLTEVDVSIGVRTQKGDIGTATGQGQGKPLVIESIDEP
                     TLSPGDYLWTSGLDRSRFPADLYVGRVTKVTKAVEGVERVVEAEPAADLTSRYVRVVL
                     REPPK"
     gene            2876..3385
                     /gene="mreD"
                     /locus_tag="VNQ33_10070"
     CDS             2876..3385
                     /gene="mreD"
                     /locus_tag="VNQ33_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWJ98494.1"
                     /translation="MSWPRTALIVLVALVLQVTLLSRFSYEGARPDLMILLAVMAGFR
                     LGPERGAIVGFAAGVSFDIVLSTPLGLSALVYTIIGYAVGAATAGMVRTSRWAAPVVA
                     AAGSAAGMLLYALVGAVLGEPTLSGPSLASIVVLVAAVNAVLAPLASRALVWARTDER
                     DRRQPFLAR"
     gene            3382..>4889
                     /locus_tag="VNQ33_10075"
     CDS             3382..>4889
                     /locus_tag="VNQ33_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWJ98495.1"
                     /translation="MTSDNTRHRLGLLAVASLSLFGALFARLWFLQIVEGDTAKAEAS
                     NNTTEVVITPAARGRILDRNGAVLVDNRQSIVVGIEAQDFSELTKGEQTAVLKRLSAT
                     LSAGRPVDQKVSVEAIRKKLDDGRYSRFRPIPIVEDISEEDQIYFAEQADLYPSVVVE
                     RTTVREYPFGSLAAHVLGYVGPLSDPQWQELSKQNDPDKPYIQTDEIGKAGVESSYEK
                     YLRGTPGRQVFEVDRTGKVVREMTKERIEPKAGDDVYLSIDAKVQYKAEEALQAQLAN
                     AYQGRSGKEAGGLVVMDQSTGQVRAMASYPTYNPADLVGGISCPTWRDLQGLPPEGSC
                     KGDSMEKEIKLLKKNDDAPVPKLLNRAYQGAYQPASTFKLASAYAALKDGVRKAGDVT
                     NDGGTVNICGGRDGPGCVKKNAGTPPAQHGPVSLSPALTVSSDVYFYKVGEDFWVRRE
                     QLGETAFQKGVEELGYGEKTGIDLPVESAGQMPTPQDEMDLAMALFEQDPAY"
BASE COUNT          783 a         1746 c         1651 g          709 t
ORIGIN      
        1 tcgaggggcg ggccgacctg ttcccggcgg cgacgtccta cagcgagccg ctccggtacg
       61 gcaacgggat gtgggccaag gtctcccgcg ccgggctcgc cgcgcagctg ggctacctcg
      121 tgttcgccgg gctgatcgcc ctcatcggtg tcgctctggt ggtcctgatc gtcgccacgc
      181 tggtggccgc ggcgacctga tctcaggttg gagcggggga tgcaggaacc cgtagggtcg
      241 gcgccactat gacgaaccgg accttcatca tgtgcaagcc cgacgccgtg gagcgcggcc
      301 tcgtcggcga ggtcatctcg cggatcgagc gcaagggcct gaccctcgcc gcggtcgagc
      361 tgcgcacggc ggacacggcg ctggccgagg cccactacgc cgagcacgcc gacaagccgt
      421 tcttcggtga gctcgtgagc ttcctcaccc gctcgccggt ggtcgccatg atcgtcgaag
      481 gccccagcga caacacctgg gagctcggcc gcatcctcat cggcaagacc caggtgaccg
      541 atgcgcagcc cggcagcatc cggggcgact tcgccaccac caccaacgag aacctggtgc
      601 acggctccga cggccccgag tccgccgagc gcgagatcgc cctctggttc ccgggtcgct
      661 gaccccgacc tgattcccct cctgaaccga acttcgtagg cgaggccatc cgttcgggcg
      721 ctctcgccta cgaagttcgg gtcgttctgg ctggtcgacc tcagcgggag gtcgagcgtg
      781 gtgacgggag gtggagggtg agactgacca tccacgtgca ggtcagcggt atctgctagg
      841 gcgattgttt caattgggtg acgacggcta gggtgtcggc gacggagctc gaagacccgg
      901 cccttgtggg gaccggagcc tgctcgtagc ctccgcaccc cgccgttccc cgccctgtgg
      961 agggcccgtc acatgtctgc tgacaacccg ctctcctcga tcatgggccg tgacatggcc
     1021 gtcgacctgg gcaccgccaa caccctcgtc tacgtccgag gccggggcat cgtcctcaac
     1081 gagccctcgg tcgtcgccgt caacgtcaaa gacggccgcc cgctcgcggt cgggctcgag
     1141 gcgaagcgga tgatcgggcg cacgccggcc cacatccagg ccatccggcc gctgaaggac
     1201 ggcgtcatcg ccgacttcga gatctgcgag aagatgctcc gctacttcat ccaccgggtg
     1261 caccagcgcc ggtgggtgaa gccccgcatg gtgatctgcg tcccgtcggg catcaccggg
     1321 gtggagcagc gggccgtgca ggaggccgcc gagtacgccg gcgcccgcaa gcccgcctac
     1381 atcatcgagg agcccatggc ggcggccatc ggcgccgggc tgccggtcca agagcccacc
     1441 ggcaacatga tcgtcgacat cggcggcggc accaccgagg tcgccgtcat ctccctcggc
     1501 ggcatcgtgt ccagccagtc ggtccgcgtc ggcggcgacg agctcgacga cgcgatcatc
     1561 cagtacatca agaaggagta cagcctggcg ctcggcgagc gcacggccga agaggtgaag
     1621 atcgccctcg gctccgcgtg gcgcctccag gaggagctgc acgccgagat ccgcggccgg
     1681 gatctggtga ccgggctgcc caagacgatc gtcaccacca ccgaggagat ccgcgaggcc
     1741 atcgaagagc ccgtcgcgtc gatctgcgat gcggtgaagg tcaccctcga caagaccccg
     1801 ccggagctgg ccgccgacat catggagcag ggcatcgtct tggccggtgg tggcgccctc
     1861 ctccacgggc tcgacgcccg cctccagcac gagacgggca tgccgatcgt gatcgcccag
     1921 aacccgctcg actgcgtggc catcgggtcc gggcagtcgc tcgaggagtt cgaggcgctc
     1981 aagggcgtcc tcttctcgac caacctggcg aactagtccg ccgcgccggc tccgtggcct
     2041 actcccgtcg cccgtcgcgc acgccccgtt cccggttcac cctggcactg ctgcttctca
     2101 ccgcggtcac gctgctggtg ctggacctgc ccggcaccgg tccgctcgac ccggtccgca
     2161 acgcgctcgg tgccgcgttc cgcccgttcc gtgccgccgg caacgccgtg ttcgaaccca
     2221 tcagcaacgg ctggaagggc gcgttcggct acgacgacct ccgagacgag aacgagcagc
     2281 tgaaggccca gctcgaggag cgcaagagcg aggaggccga cgtcaagcgc ctccagcaga
     2341 aggtcgcgga cctgagcaag atcaacgacg tccgagtcga gcagcagaag acccgggtcg
     2401 ccgaagtggt ttcgggtccg ctcaacagct tcgagcagac cctcgagatc gacgccggct
     2461 ccagcgacgg catcaagaag ggcatggcgg tcatcacggg ccgggcgcgg gggaaaggcg
     2521 gcgccgtcct cggccggatc agcgaggtcc gcagcggctc gtcgacggtc cagctcctca
     2581 ccgaggtcga cgtgtcgatc ggggtccgga cccagaaggg cgacatcggc accgccacag
     2641 gccaggggca gggcaagccc ctggtcatcg agagcatcga cgagccgacg ctgagcccgg
     2701 gcgactacct gtggacctcg ggcctcgacc gctcgcgctt tcccgcagat ctctacgtgg
     2761 gccgggtcac caaggtcacc aaggccgtcg aaggcgtcga gcgcgtggtg gaggccgagc
     2821 ccgcggccga cctgacgtct cggtacgtgc gggtcgtcct ccgggagccg ccgaagtgag
     2881 ctggccccgc accgcgctca tcgtcttggt ggccctggtg ctccaggtca cgctgctctc
     2941 gcggttctcc tacgaggggg cccgccccga cctgatgatc ctgctggcgg tgatggccgg
     3001 cttccgcctc ggtcccgagc gcggcgccat cgtcgggttc gccgccggcg tgtccttcga
     3061 catcgtgctg tcgacgcccc tcggcctgtc cgccctcgtg tacacgatca tcggctatgc
     3121 ggtcggcgcc gccacggccg ggatggtgcg cacgtcgcgc tgggcggctc cggtggtggc
     3181 cgccgccgga tcggcggcgg ggatgctgct gtatgcgctg gtcggcgcgg tgttgggcga
     3241 gccgaccctc agcggaccct ccctcgcctc tatcgtcgtg ctcgtggcgg cggtgaacgc
     3301 cgtcctcgct cccctcgcct cccgggcgct cgtgtgggcc cgcaccgacg agcgcgaccg
     3361 gcgccaaccc ttcctcgcac gatgacctcg gacaacaccc gccaccgcct cggactcctc
     3421 gccgtggcgt cgctgtcgct cttcggcgcc ctcttcgccc ggctctggtt cctccagatc
     3481 gtcgagggcg acaccgccaa ggcggaggcc agcaacaaca ccaccgaggt cgtcatcacc
     3541 ccggcagcgc gcggccggat cctcgaccgc aacggtgcgg tcctggtcga caaccgccag
     3601 tccatcgtcg tcggcatcga ggcgcaggac ttcagcgagc tcacgaaggg cgagcagacg
     3661 gcggtgctca agcggctgtc ggccaccttg agcgccggtc gtccggtcga ccagaaggtc
     3721 tccgtcgagg ccatccgcaa gaagctggac gacggccgct actcccggtt ccggccgatc
     3781 ccgatcgtcg aggacatctc cgaggaggac cagatctact tcgccgagca ggccgacctc
     3841 tacccgtcgg tcgtcgtcga gcgcaccacc gttcgcgagt accccttcgg ctcgctcgcc
     3901 gctcacgtgc tcggctacgt ggggccgctc agcgatccgc agtggcagga gctctcgaag
     3961 cagaacgatc ccgacaagcc gtacatccag accgacgaga tcggcaaggc cggggtcgag
     4021 tcctcctacg agaagtacct gcgggggacc cccggtcgac aggtcttcga ggtggaccgc
     4081 accggcaagg tcgtccggga gatgacgaag gaacggatcg agccgaaggc cggcgacgac
     4141 gtgtacctgt cgatcgacgc caaggtgcag tacaaggccg aggaggcgct ccaggcccag
     4201 ctggcgaacg cctaccaggg gcgttcgggc aaggaggccg gcggcctcgt ggtgatggac
     4261 cagagcacgg gccaggtgcg ggccatggcg tcgtacccca cctacaaccc ggccgacctg
     4321 gtgggcggga tcagctgccc gacctggcgc gacctccagg gcctgccgcc cgagggctcg
     4381 tgcaagggcg acagcatgga gaaggagatc aagctcctca agaagaacga cgacgcaccc
     4441 gtgccgaagc tgctcaaccg ggcgtaccag ggcgcctacc agccggcctc gacgttcaag
     4501 ctggccagcg cctacgccgc cctcaaagac ggcgtgcgca aggcgggcga cgtgaccaac
     4561 gacgggggca cggtcaacat ctgcggcggg cgcgacggcc ccggctgcgt caagaagaac
     4621 gccggcaccc ctcccgccca gcacggtccg gtcagcctca gcccggcgct cacggtctcc
     4681 agcgacgtgt acttctacaa ggtcggcgag gacttctggg tccgtcgcga gcagctcggc
     4741 gagacggcct tccaaaaggg cgtcgaggag ctcggctacg gcgagaagac cggcatcgac
     4801 ctccccgtcg agtcggccgg ccagatgccc accccgcaag acgagatgga cctggcgatg
     4861 gcgctcttcg agcaggaccc ggcctacta
//
LOCUS       DATMFS010000536         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_113155, whole genome shotgun sequence.
ACCESSION   DATMFS010000536 DATMFS010000000
VERSION     DATMFS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113155"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VNQ33_10080"
     CDS             <1..652
                     /locus_tag="VNQ33_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ98496.1"
                     /translation="DPVPDVYFAGSSPAALDVAARHADVYLTWGEPPAQVAEKLDRVR
                     ELAAAQGRTPRFGIRLHVVSRDTSDEAWKVANGLLEGISEEEIAKVHARMKTTESEGQ
                     RRMLELHGGSRDNLEVSPNLWAGVGLARGGAGTALVGSHAEVADRIEEYAALGIDEFI
                     LSGYPHLEEVYWFGEGVLPVLEDRGSWTRPVGSAEGMRTGSIASGGVSFGVVPERR"
     gene            788..1237
                     /gene="rplM"
                     /locus_tag="VNQ33_10085"
     CDS             788..1237
                     /gene="rplM"
                     /locus_tag="VNQ33_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWJ98497.1"
                     /translation="MPTYTPKASEIERNWLIIDADGLVLGRLATEVAAILRGKHKPTF
                     APHIDTGDHVVIINCEKIVLTAGKAEKKKVFRHSGFPGGIKEQTFADLLEKKPEDAVR
                     NTVRGMLPKNRLGRAQLKKLKVYAGPNHPHAAQQPQVLELDHARRSA"
     gene            1249..1653
                     /gene="rpsI"
                     /locus_tag="VNQ33_10090"
     CDS             1249..1653
                     /gene="rpsI"
                     /locus_tag="VNQ33_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWJ98498.1"
                     /translation="MSKPLVQSTGRRKEAVARVRLRPSEGDAEATITINGRDWVDFFP
                     NPTHRMILQEPLKLTQLEESYDVDVTIHGGGVTGQAGAVRLGIARALIELDPELRPTL
                     KRAGFLTRDDRKKESKKYGLKKARKAPQYSKR"
     gene            1751..>2260
                     /locus_tag="VNQ33_10095"
     CDS             1751..>2260
                     /locus_tag="VNQ33_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWJ98499.1"
                     /translation="MSLKFGTDGVRGIANRELTPELALNLGRAAARVLGGARWLIGRD
                     TRRSGPLLSAALAAGLATEGVEVVDLGVVPTPGVAFLSAADHVPAAMISASHNPFGDN
                     GIKLFSAGGLKLSDELEERLELELTALSGQGDPRPRPTGTEVGTITTDHGAIDRYRRF
                     LAEDVVPAGS"
BASE COUNT          344 a          851 c          752 g          313 t
ORIGIN      
        1 cgacccggtc cccgacgtgt acttcgccgg ctcgtcgccg gctgcgctcg atgtcgccgc
       61 ccgccacgcc gacgtctacc tcacgtgggg cgagccgccc gcccaggtgg ccgagaagct
      121 cgaccgggtc cgcgagctgg ccgccgccca gggccgcacc ccgcgcttcg gcatccggct
      181 ccacgtcgtg agccgcgaca cgtccgacga ggcctggaag gtggcgaacg gcctcctcga
      241 gggcatctcc gaggaggaga tcgccaaggt ccacgcccgg atgaagacca ccgagtccga
      301 gggccagcgg cgcatgctcg agctccacgg cgggtcccga gacaacctcg aggtctcgcc
      361 caacctgtgg gccggcgtcg ggttggcccg cgggggcgcg ggcaccgccc tggtggggag
      421 ccacgccgag gtcgccgacc gcatcgagga gtacgccgcg ctcggcatcg acgagttcat
      481 cctctcgggc tacccgcacc tcgaggaggt ctactggttc ggcgagggcg tcttgcccgt
      541 gctcgaggac cgcggctcgt ggacccggcc cgtcggctcg gccgagggca tgcgcaccgg
      601 ctcgatcgcc tcgggtggcg tctcgttcgg cgtggtcccc gagcggaggt agccccgccg
      661 gtccaccacc ggccgggcgc gctctgctat ttgccctggc agaccggcgg gccgtaacgt
      721 atcccttcgg cccgaagccg ccctgggggc gattctggct cacgaccagc agatccaagg
      781 attcaccgtg cccacgtaca cgcccaaagc ttcagagatc gagcgcaact ggctgatcat
      841 cgatgccgat ggcctggtgc tcggccgcct cgccaccgag gtcgccgcca tcttgcgtgg
      901 caagcacaag cccacgttcg ccccccacat cgacaccggg gaccacgtgg tcatcatcaa
      961 ctgcgagaag atcgtcctca cggccggcaa ggccgagaag aagaaggtgt tccgccactc
     1021 cgggttcccg ggcggcatca aggagcagac cttcgccgac ctcctggaga agaagccgga
     1081 agacgcggtg cgcaacaccg tgcgcggcat gctcccgaag aaccggctcg gccgggccca
     1141 gctcaagaag ctgaaggtct acgccggccc caaccacccg catgccgctc agcagcccca
     1201 ggtgctcgag ctcgaccacg cccgccgcag cgcgtaggag tccctgacgt gtccaagccc
     1261 ctcgtgcagt ccaccggtcg ccgcaaggaa gccgtcgctc gcgtccgcct ccgtccctcc
     1321 gagggcgatg cggaagccac catcaccatc aacggccgcg actgggtcga cttcttcccg
     1381 aacccgacgc accggatgat cctccaagag cccctcaagc tgacccagct cgaggagtcc
     1441 tacgacgtcg acgtcaccat ccacggtggc ggcgtcaccg gccaggccgg cgccgtgcgc
     1501 ctcggcatcg cccgggccct catcgagctc gaccccgagc ttcgccccac cctcaagcgc
     1561 gccgggttcc tgacccgaga cgatcgcaag aaggagtcca agaagtacgg cctcaagaag
     1621 gcccgcaagg ctcctcagta ctcgaagcgc tagtccgctt cctgcctctg gtcgaaggcc
     1681 gcagctccct cggagctgcg gccttcgtcg tttccgggac ccggctgcgc gcgccagtac
     1741 ggtggctcgc atgagcttga agttcgggac ggacggggtc cgcgggatcg ccaaccggga
     1801 gctgacgccg gagctggcgc tgaacctggg ccgggccgcg gcccgggtgc tcggcggggc
     1861 tcgctggctg atcgggcgtg acacccgccg ttcggggccg ctgctgtcgg ccgccctcgc
     1921 cgccgggctg gccaccgagg gcgtcgaggt cgtcgacctg ggcgtcgtgc ccacgccggg
     1981 cgtcgccttc ttgtcggcgg ccgaccacgt gccggccgcg atgatctcgg cgtcgcacaa
     2041 ccccttcggc gacaacggca tcaagctgtt ctcggccggt gggctgaagc tgtccgacga
     2101 gctcgaggag cgcctggagc tggagctcac cgcgctgagc ggccagggcg acccccggcc
     2161 ccggcccacc ggcaccgagg tgggcacgat caccaccgac cacggtgcca tcgaccgcta
     2221 ccgccggttc ctggccgaag acgtggtccc tgccgggtcg
//
LOCUS       DATMFS010000537         7199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_18805, whole genome shotgun sequence.
ACCESSION   DATMFS010000537 DATMFS010000000
VERSION     DATMFS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18805"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNQ33_10100"
     CDS             complement(<1..697)
                     /locus_tag="VNQ33_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWJ98500.1"
                     /translation="MARAKFQAPKGTQDVLPPVSARWERLLAAYAQTVERAGYGLLQS
                     PMFEEIGVFQRESEGADAVRKEMYDFDDKGGRRIALRPEGTASVVRAFVQHQPASPWK
                     VWYATPAFRYENVQAGRFRQHHQVGLEVLGSADPDVDVEVITLGWEFLGALGLRQVEL
                     LLNTMGTAADRVRYVEVLRSYLQDRIGDLATEDREKVERHPMRVLDSKRTETRRATAD
                     APAIADHLSDESAE"
     gene            complement(804..1721)
                     /locus_tag="VNQ33_10105"
     CDS             complement(804..1721)
                     /locus_tag="VNQ33_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98501.1"
                     /translation="MVADVDSLIADDAPGWFRRALHTPFFDEHVEVDGARIHYLAWGE
                     PGRKGLVFVHGGAAHAHWWTHVAATFSRQYRVVAIDLSGHGDSEHRDTYSLEQWSREV
                     MAVADDAGIGGPPVIVGHSMGGFVTIATAALHSDRLAGVIVLDSPVAEPDAEVGAAQA
                     KQAFGVPKVYPTKEDALLRFRTVPRQENDLPYVIHHVARRSLHEVEGGWSWKFDHNLF
                     SAFTSSIRAVALPYLPEVRCRFALLRSELGLVTPDIGESMYEKLGRVAPVVELPETGH
                     HGMLDQPLLVITAIRTLLADWDHSTPQRR"
     gene            complement(1741..3966)
                     /locus_tag="VNQ33_10110"
     CDS             complement(1741..3966)
                     /locus_tag="VNQ33_10110"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWJ98502.1"
                     /translation="MSTVSRVLPWRRSSAPASVELAPLLATFRVHHPKKSTVLIAQAY
                     ELAAAAHEGQTRKSGEPYIHHPLSVARIVADLGLDDVTVAAALLHDSVEDTGVTLEQV
                     ARQFGDEVAAIVDGVTKLDRVQFDSKAAQQAASMRKMLVAMAKDLRVLMIKLADRLHN
                     MRTLGAMAPEKQTRIARETIDVYAPLAHRLGMQDLKQQLEDLSFATLHPKQYAEIDHM
                     VAERSPERELYLEQVLEEVRQRLAELGVEADVSGRPKHLWSIYEKMVVKGRQFDEIYD
                     LVGIRVTVGSVRDCYAALGSIHATWRPVQGRFKDYVAMPKFNLYQSLHTTVIGPQGKP
                     LEVQLRTLEMHQRAEFGVAAHFAYKRGTPADELAWLNRIVDWQQETSDPEQFMETLKV
                     DLEQDEVYVFTPKGRVVTMVKGATPIDFAYAVHTEVGHACVGARVNGRLVALSHQLTS
                     GDTCEIFTSKVEGSGPSRDWLQIVQTPRAANKIRQWFSRERREDARETGREELQKQLR
                     KEGLPTQKLPNGILEEAALDLNYLDADALYTAIGEHHVSPEAVVARLNRLLHSGDPER
                     VDQLPTSVTRTSSMRSNRTGAGVHVEGLDDVMVRLSRCCTPVPGDEIIGFVTRGRGVS
                     VHRADCANAASLQAGQRDRLMEVEWDSDLSGGIFVASVEVRSLDRPGLLGDVSGVLAE
                     HKVNIIACNMKTDRDRMALMRFDCELGDPSHLDAVLGRIRSIDGVYQSYRVLPGAGTS
                     D"
     gene            complement(4028..4555)
                     /locus_tag="VNQ33_10115"
     CDS             complement(4028..4555)
                     /locus_tag="VNQ33_10115"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWJ98503.1"
                     /translation="MPAPVDITAYIRDIDDFPKPGITFKDITPLLAHPEALATAVEGL
                     SEPFQDLGITKVVGMEARGFIFAAPVAVAIGAGFVPVRKAGKLPWAVHRQEYQLEYGT
                     DLLEIHQDALAADDRVLIVDDVLATGGTAAATVELVGQSGAEVVALGFVIELGFLGGR
                     PALGDARVRALVTYP"
     gene            complement(4555..6126)
                     /gene="secF"
                     /locus_tag="VNQ33_10120"
     CDS             complement(4555..6126)
                     /gene="secF"
                     /locus_tag="VNQ33_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWJ98504.1"
                     /translation="MTNPSDPVDPTGDEPVESVEPESVKPEAVDTDAVDGADAADGPQ
                     EEIVEDAPVDSEDDLMAIAQAPVHHGALSRLYHGETNINMVGRWKTWFAISGAFLLIG
                     LVALVGSGLNLGIDFTGGTVWEVPAGKADVAKVTAAMSDLGYNDVQVQLVTQNTGSGD
                     KRIMRVEAEATASPDQATTKAIDEAKAGLNSIKGKLKGESAQRIEAVRANLDSIEGPF
                     AKPVPDELKALQDQIDELADQLPGSKDKTKLVSSAASDMQTQVSALDAAEQAERERTG
                     QAVSDELAAQTGSNVKEVTVDTVGPSWGQQVSGKARTALIVFLIAISIYITLRFEFRM
                     AMATLAALIHDLLIVVGIYAIFQFPVTPATVIALLTILGFSIYDGIVVFDRVDENTKL
                     LGRKSRMTYSEMANMSLNQVLMRSLNTSITALLPIASVLVLGSFVLGATTLEEFGLAL
                     FLGLLSGAYSSIFIATPLLAILKEREPRYRELRERLAARGPQGEELVTAGAGSSASSR
                     TADVTPRARKQGRTR"
     gene            complement(6123..>7199)
                     /locus_tag="VNQ33_10125"
     CDS             complement(6123..>7199)
                     /locus_tag="VNQ33_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HWJ98505.1"
                     /translation="ASTSQTNGCNSGSKVDTSTVSTTAPLPEGAEIVPSKEGTLLYTL
                     GPVGFTGDALSKAEAALQESWAVNVSVRGAKKAEANAAFNACYNSEPTCPAQGQGGRG
                     AIAIVLDGEVLSAPAVNGPDLASDNFTISGDFDQAEAKELALVLRYGSLPVEFDQVAL
                     QQVSATLGTDSLHAGLLAGAVGIALVAAYVLLYYRGLGLVVVGGLAVWGGFMYGLVCW
                     LSANQGLALTLSGITGIVVSVGTTVDSYVVYFERMRDEVRAGRSARSATDRGFSSAIR
                     TIITADVASFLGAFLLWWLTVGPVRGFAFFLGMSVVLDLFVAYFFTRPVVALLSYNKG
                     LIESKFMGLESARSRAVDTNGATS"
BASE COUNT         1048 a         2412 c         2625 g         1114 t
ORIGIN      
        1 gctcggcgct ctcgtccgac aggtggtcgg cgatggccgg ggcatcggcg gtggctctgc
       61 gggtctcggt gcgcttcgag tccaggaccc gcatggggtg gcgctcgacc ttctcgcggt
      121 cctcggtggc caggtcgccg atccggtcct gcaggtacga gcggagcacc tcgacgtagc
      181 ggacgcggtc ggcggcggtg cccatggtgt tgagcagcag ctcgacctgg cgcagcccca
      241 gggcaccgag gaactcccag ccgagggtga tgacctcgac gtcgacgtcg gggtcggccg
      301 agccgagcac ctcgaggccg acctggtggt gctggcggaa ccggccggcc tgcacgttct
      361 cgtagcggaa cgccggcgtg gcgtaccaga ccttccacgg cgaagccggc tggtgctgca
      421 cgaaggcccg caccaccgat gcggtcccct cggggcgcag ggcgatgcgg cgaccgccct
      481 tgtcgtcgaa gtcgtacatc tccttgcgga ccgcgtccgc cccctcggac tcccgctgga
      541 agaccccgat ctcctcgaac atgggcgact ggagcagccc gtacccggcc cgctcgacgg
      601 tctgcgcgta ggccgcgagc aggcgttccc accgtgctga caccggggga aggacgtctt
      661 gggtcccctt cggtgcctgg aacttcgctc gtgccatgag gggtcgagcg tacccgtcgc
      721 tcctcccccg cccgaaacga acttcgtagg ccagaacgcc cgaaacggcg ccctccccta
      781 cgaagttcgg ctggtggggt gggtcagcgc cgctgcggcg tgctgtggtc ccagtcggcc
      841 agcagggtgc ggatggcggt gatgacgagg aggggctggt cgagcatgcc gtggtggccc
      901 gtctcgggga gctccacgac cggggcgacc cgtccgagct tctcgtacat cgactcgccg
      961 atgtcgggcg tgaccaggcc cagctcggag cggagcaggg cgaaccggca gcggacctcg
     1021 ggcaggtacg gcagcgccac cgcccggatg ctggacgtga aggcgctgaa gaggttgtgg
     1081 tcgaacttcc agctccagcc gccctcgacc tcgtggagcg accggcgggc gacgtggtgg
     1141 atcacgtacg gcaggtcgtt ctcctggcgc gggacggtgc ggaagcgcag gagggcgtcc
     1201 tccttcgtgg ggtacacctt cggcacgccg aaggcctgct tggcctgggc ggcgccgacc
     1261 tcggcgtcgg gctcggccac gggtgagtcc agcacgatca caccggccag ccgatcggag
     1321 tggagggcgg cggtggcgat ggtgacgaac ccgcccatgc tgtggcccac gatcacgggc
     1381 ggtcccccga tgccggcatc gtcggcgacg gccatcacct cgcgggacca ctgctcgagc
     1441 gagtaggtgt cgcggtgctc ggagtcgccg tgccccgaca ggtcgatggc gacgacgcgg
     1501 tactgccggg agaacgtggc ggccacgtgg gtccaccagt gggcgtgggc ggcgccgccg
     1561 tggacgaaca ccaggccctt gcgcccgggc tcgccccagg ccaggtagtg gatgcgggcg
     1621 ccgtcgacct cgacgtgctc gtcgaagaac ggcgtgtgca gcgcccgccg gaaccacccg
     1681 ggagcgtcgt cggcgatcag cgagtccacg tcggcaacca tcggtcgcac ggtagaccag
     1741 tcagtcgctg gttccggccc ccggcaggac gcggtacgac tggtagacgc cgtcgatcga
     1801 gcggatgcga cccagcacgg catcgaggtg ggaggggtct cccagctcgc agtcgaaccg
     1861 catcaaggcc atgcggtccc ggtcggtctt catgttgcag gcgatgatgt tcaccttgtg
     1921 ctcggcgagc acgcccgaca cgtcgcccag gaggccgggc cggtcgagcg agcgcacctc
     1981 gacggacgcc acgaagatgc cgcccgacag gtcgctgtcc cactcgacct ccatcagccg
     2041 gtcgcgctgg ccggcttgga gcgaagcggc gttggcgcag tcggcccggt gcaccgacac
     2101 gccgcggccg cgggtgacga agccgatgat ctcgtcgccg ggcaccggtg tgcagcagcg
     2161 cgagagccgg accatgacgt cgtcgaggcc ctcgacgtgg acacccgcgc ccgtgcggtt
     2221 gctgcgcatc gacgacgtcc gcgtgacgga ggtgggcagc tggtcgaccc gctccgggtc
     2281 accggagtgg aggaggcggt tgaggcgggc caccaccgcc tcgggcgaga cgtggtgctc
     2341 gccgatggcg gtgtagagcg cgtcggcgtc gaggtagttg aggtcgagcg cggcttcctc
     2401 gaggatgccg ttcgggagct tctgggtggg caggccctcc ttccggagct gcttctgcag
     2461 ctcctcccgc ccggtctcac gggcatcctc gcggcgctca cgggagaacc actgccggat
     2521 cttgttggcg gcccgcggcg tctggacgat ctggagccag tcccggctgg gtccgctccc
     2581 ctccaccttc gaggtgaaga tctcgcacgt gtcgcccgac gtgagctggt ggctgagggc
     2641 tacaaggcgc ccgttcacgc gagcgccgac gcaggcgtgg ccgacctcgg tgtgcaccgc
     2701 gtaggcgaag tcgatgggcg tcgccccctt gaccatggtg acgacccgcc ccttgggggt
     2761 gaagacgtag acctcgtctt gctccaggtc gaccttgagc gtctccatga actgctcggg
     2821 gtccgacgtc tcctgctgcc agtcgacgat gcggttgagc cacgccagct cgtcggccgg
     2881 ggtgccgcgc ttgtaggcga agtgggcggc gacgccgaac tcggcgcgct ggtgcatctc
     2941 gagcgtgcgc agctgcacct cgagcggctt gccctgcggc ccgatcacgg tcgtgtgcag
     3001 cgactggtac aggttgaact tgggcatggc cacgtagtcc ttgaaccggc cctgcaccgg
     3061 gcgccacgtg gcgtggatcg agccgagggc ggcgtagcag tcccgcaccg agcccaccgt
     3121 gacgcggatc ccgacgaggt cgtagatctc gtcgaactgg cggcccttga cgaccatctt
     3181 ctcgtagatg ctccacaggt gcttcggccg gccggagacg tcggcctcca cacccagctc
     3241 ggccagccgc tggcgcacct cctcgagcac ctgctccagg tacagctcgc gctcgggcga
     3301 gcgttcggcg accatgtggt cgatctcggc gtactgcttg gggtgcagcg tggcgaagct
     3361 gaggtcctcg agctgctgct tgaggtcctg catgccgagc cggtgcgcga gcggcgcgta
     3421 gacgtcgatg gtctcgcggg cgatgcgggt ctgcttctcg ggcgccatgg cgccgagcgt
     3481 ccgcatgttg tggagccggt cggcgagctt gatcatcagg acccgcaggt ccttggccat
     3541 ggcgacgagc atcttgcgca tcgacgccgc ctgctgggcg gccttcgagt cgaactggac
     3601 ccggtcgagc ttggtgacgc cgtcaacgat ggccgccacc tcgtcgccga actgccgggc
     3661 tacctgctcc agcgtgacgc cggtgtcctc gacggagtcg tgcaggaggg cggcggcgac
     3721 ggtgacgtcg tcgaggccga ggtcggccac gatgcgggca accgagagcg gatggtggat
     3781 gtacggctcg ccgctcttgc gggtctggcc ctcgtgggct gcggcggcca gctcgtaggc
     3841 ctgggcgatc aggaccgtcg acttcttcgg atggtgcacc cggaaggtgg ccagcagggg
     3901 cgcgagctcg accgaggcgg gggcgctgga gcggcgccac gggagcacgc ggctgacggt
     3961 cgacatggct ccagcctacg gcgcccccgc aaccgccgga ccgggtggtt ccgaggcgcc
     4021 cgccgcgtca ggggtaggtg acgagcgccc tcacccgagc gtcgccgagc gccgggcgac
     4081 cgccgaggaa gccgagctcg atgacgaacc ccagggcgac gacctcggca ccgctctgtc
     4141 cgaccagctc gaccgtcgcc gccgcggtac cgcccgtcgc caagacgtcg tcgacgatca
     4201 ggacgcgatc atcggccgcg agcgcgtcct ggtggatctc caggagatcc gtgccgtact
     4261 ccagctggta ctcctggcgg tgcaccgccc acgggagctt gccggccttg cggacgggca
     4321 cgaatcccgc tccgatcgcg acggcgacgg gagcggcgaa gatgaacccg cgcgcctcca
     4381 tgccgacgac cttggtgatg ccgaggtcct ggaacggttc gctcaggccc tccaccgcgg
     4441 tggcgagcgc ctccgggtgg gcgagcagcg gcgtgatgtc cttgaaggtg atccccggct
     4501 tcgggaagtc gtcgatgtcg cggatgtagg ccgtgatgtc gaccggtgcg ggcatcagcg
     4561 cgtccggccc tgcttgcggg cccggggcgt gacgtcggcg gtccgggacg aggccgacga
     4621 cccggcgccg gcggtgacca gctcctcgcc ctgcggaccc cgggcggcca gccgctcacg
     4681 cagctcgcgg taccggggct cgcgctcctt caagatggcg agcagcgggg tggcgatgaa
     4741 gatcgacgag taggcgccgg acaggaggcc gaggaacagg gccaggccga actcctcgag
     4801 cgtcgtggcg cccagcacga acgatcccag caccagcacc gacgcgatcg gcaagagcgc
     4861 ggtgatcgac gtgttgagcg accgcatgag cacctggttc agggacatgt tggccatctc
     4921 ggagtacgtc atccgcgact tccggccgag cagcttggtg ttctcgtcga cgcggtcgaa
     4981 caccacgatg ccgtcgtaga tcgagaagcc gaggatggtg agcagcgcga tcaccgtcgc
     5041 cggggtcacc gggaactgga agatggcgta gatgcccacc acgatcagca ggtcgtggat
     5101 gagggcggcg agggtggcca tggccatgcg gaactcgaac cgcagggtga tgtagatgct
     5161 gatcgcgatg aggaacacga tcagggccgt acgggccttg cccgacacct gctggcccca
     5221 cgagggaccc accgtgtcga ccgtgacctc cttgacgttc gatcccgtct gagcggcgag
     5281 ctcgtcggag acggcctggc ccgtgcgctc ccgctcggcc tgctcggcgg catcgagggc
     5341 gctgacctgg gtctgcatgt cggacgccgc gctgctgacc agcttggtct tgtccttcga
     5401 gccgggcagc tggtcggcca gctcgtcgat ctggtcctgc agggccttca gctcgtcggg
     5461 caccggcttg gcgaaggggc cctcgatgct gtcgaggttg gcgcgcacgg cctcgatccg
     5521 ctgagccgac tcccccttga gcttgccctt gatcgagttg aggcccgcct tcgcctcgtc
     5581 gatggccttg gtggtggcct gatcgggcga tgcggtcgcc tcggcttcga cgcgcatgat
     5641 gcgcttgtcg cccgacccgg tgttctgggt gacgagctgg acctgcacgt cgttgtagcc
     5701 gaggtcgctc atcgccgcgg tgaccttggc gacgtcggcc ttgcccgccg ggacctccca
     5761 caccgtgccg ccggtgaagt cgatgccgag gttcagcccg ctgcccacca gggcgacgag
     5821 accgatgagc aagaacgcgc cggagatggc gaaccacgtc ttccagcggc cgaccatgtt
     5881 gatgttggtc tcgccgtggt acagccggga cagggcgccg tggtggacgg gcgcctgggc
     5941 gatggccatc aggtcgtctt cggagtcgac cggcgcatcc tcgacgatct cctcctgcgg
     6001 cccgtcggca gcatcggctc catcgaccgc gtcggtgtcg acggcttcgg gcttgacgga
     6061 ctcgggctcg acggactcga caggctcgtc gccggtgggg tccacggggt cgctggggtt
     6121 ggtcacgacg tcgctccgtt cgtgtcgacc gctcggcttc tggcgctctc gagacccatg
     6181 aacttcgact cgatcaggcc cttgttgtag ctgagcagcg ccaccaccgg ccgcgtgaag
     6241 aagtaggcga cgaacaagtc gaggaccacc gacatgccca ggaagaaggc gaagccgcgg
     6301 acgggcccga cggtcagcca ccacagcagg aaggcaccca ggaacgaggc cacgtcggcg
     6361 gtgatgatcg tccggatggc gctgctgaac ccgcggtcgg tggcactgcg ggcgctgcgc
     6421 ccggcgcgca cctcgtcacg catgcgctcg aagtacacca cgtaggagtc cacggtggtg
     6481 ccgaccgata cgacgatgcc ggtgatgccc gacagcgtca gcgccaggcc ctggttggct
     6541 gacaaccagc agaccaggcc gtacatgaac ccgccccaga cggcgaggcc gccgaccacg
     6601 acgaggccga gaccccggta gtacagcagc acgtaggccg cgacgagggc gatgccgacg
     6661 gcgccggcga ggaggccggc gtgcagcgag tcggtgccga gcgtggcgga cacctgctgg
     6721 agggcgacct ggtcgaactc caccggcagg gagccgtagc gcagcacgag ggccagctcc
     6781 ttggcctcgg cctggtcgaa gtcgccgctg atggtgaagt tgtcgctcgc caggtcagga
     6841 ccgttcacag ccggcgccga cagcacctcg ccgtcgagga cgatggcgat ggcaccccgg
     6901 ccgccctgcc cctgggccgg gcaggtgggc tcgctgttgt agcaagcgtt gaaggcggcg
     6961 ttggcctcgg ccttcttggc cccgcggacc gacacgttga ccgcccagct ctcctgcagg
     7021 gcggcctcgg ccttgctcag cgcgtcaccc gtgaagccca ccgggccgag cgtgtagagg
     7081 agcgtgccct ccttggacgg gacgatctcg gcgccctcgg gcagcggggc ggtggtcgac
     7141 accgtggagg tgtcgacctt gctgccggag ttgcagccgt tggtctggct ggtgctggc
//
LOCUS       DATMFS010000538         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_178750, whole genome shotgun sequence.
ACCESSION   DATMFS010000538 DATMFS010000000
VERSION     DATMFS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178750"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2095)
                     /locus_tag="VNQ33_10130"
     CDS             complement(<1..>2095)
                     /locus_tag="VNQ33_10130"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HWJ98506.1"
                     /translation="VKDGKRRYDGFVVERPAVSTQLPVLDWYLSDADAQWLAENRGSK
                     EYVPAVLVVDGRVIDGVEVRNQGGATSAFSIKPNFKFKLPGQQTIAEPFFDAPVDELV
                     IDADFEDPTSAIGRTAWSLMAEAGVPRRRAATGRVERNGAFFGVFQLYQEYDGTWFDQ
                     LPYEMASFYEGESGRADLLDEGDPAALAERWEQTEGDGTAAELTELVAAVDRSAGPQR
                     VDAVVDRFDVPRLVDLIAMSKLVQHLDTGRTNMDLVWGADGRWRIIPNDLDLTLGLRG
                     RTRYQGFVPTPLTSVTSLLSTEGELADQVARRTRTLADEWFEDDRILDDVRARVDAMR
                     PDLELDRQRWVYDRISPDEGLADLADFLETQRAVLDGWTATGVIPGPGTEGTEVRVSE
                     LAAHGSADDFVELANPDRARSIDVSGWTLGGAATATLPGGSVLAPGARLVVPASAAAV
                     AARRRGTVVSGELDAALPPAGGTLVVRDAEGGVVDQVTWRTTGGWPDPGSSGRSIEAR
                     SLDAGRALPSGWALSPDAGGTPGAAGASERGLSVRVDAGVEAGPIGAPLPIAVVIANA
                     GPGARTGVEVDGPGTCDRSVDAIPPGGVVVLHCEGASTQALGFEPLRTVVRAAEEPDG
                     VVDAPVVRHVPESILPGVPELLQVRLEANRIRVGYRATGAASEPGEAPWRVEAVAVPS
                     GAGEGRGAVVGAGTA"
BASE COUNT          278 a          813 c          730 g          274 t
ORIGIN      
        1 tcggccgtcc ccgcacccac gaccgcgccg cgcccctccc ctgcgccgct gggaacggcg
       61 acggcctcga cccgccacgg cgcctccccg ggctccgacg cggcaccggt cgcccggtag
      121 cccacacgga tgcggttcgc ctccagccgg acctggagga gctccggcac gccgggcaga
      181 atcgactctg gaacgtgacg caccaccggt gcgtcgacca cgccgtcagg ctcctcagcc
      241 gcccgcacga cggtccggag cggctcgaac cccagcgcct gcgtcgaagc accctcgcag
      301 tggaggacca cgacaccgcc gggcgggatc gcgtcgacgg aacggtcgca cgtgccgggt
      361 ccgtcgacct cgacccccgt ccgggctccc ggcccggcgt tggcgatgac gaccgcgatc
      421 ggcagcggtg cgccgatggg gccggcctcg accccagcat cgacccggac gctcagcccc
      481 cgctcgctcg ccccggccgc tccgggcgtg ccgccggcat ccggcgacag ggcccacccc
      541 gacgggaggg ctcgtccggc atcgagcgag cgcgcctcga tcgagcgccc cgatgagccc
      601 gggtcaggcc agccgcccgt ggttctccag gtcacctggt ccaccacgcc gccctcggcg
      661 tcgcggacca cgagggtgcc ccctgccggt gggagggcgg cgtcgagctc gccgctgacc
      721 acggtgcctc gccgccgcgc cgcgaccgcg gccgcggagg cgggcacgac gagacgggcg
      781 cccggcgcca gcaccgagcc accaggcagc gtcgccgtcg ccgcgccgcc gagcgtccag
      841 ccggagacgt cgatcgatcg agcgcgatcg gggttggcga gctccacgaa gtcgtcggcg
      901 ctcccgtgcg cggccaactc ggagacgcgg acctcggtgc cctcggtccc cggtcccggg
      961 atcaccccgg tggcggtcca cccgtcgagc accgctcgct gggtctcgag gaagtcggcc
     1021 aggtcggcga ggccctcgtc ggggctgatc cggtcgtaca cccagcgctg tcggtccagt
     1081 tccaggtccg ggcgcatggc gtcgacgcgg gcccggacgt catcgaggat ccggtcgtcc
     1141 tcgaaccact cgtcggcgag ggtgcgggtc cgtctcgcca cctggtccgc cagctcgccc
     1201 tcggtgctca gcaggctcgt cacgctggtg agcggggtcg ggacgaaccc ctggtaccgg
     1261 gtcctcccgc gcagcccgag cgtcaggtcg aggtcgttgg ggatgatccg ccagcggcca
     1321 tcggcgcccc acaccagatc catgttcgtg cggcccgtgt cgaggtgctg caccagcttg
     1381 gacatcgcga tcaggtcgac gagacggggc acgtcgaagc ggtccaccac ggcatccacg
     1441 cgctgggggc ccgcactgcg gtccaccgcg gccaccagct cggtcagctc cgctgcggtg
     1501 ccgtcgcctt cggtctgctc ccagcgctca gcgagcgccg ccgggtcgcc ctcgtccagg
     1561 aggtcggcgc ggccggattc cccctcgtag aaggaggcca tctcgtacgg gagctggtcg
     1621 aaccacgtcc cgtcgtactc ctggtagagc tggaacacgc cgaagaacgc gccgttgcgc
     1681 tcgacgcggc ccgtcgcggc ccggcgccgc ggaacccccg cctcggccat cagcgaccag
     1741 gcggtccggc cgatcgccga agtcggatcc tcgaagtcgg cgtcgatgac gagctcgtcg
     1801 acgggtgcat cgaagaacgg ctcggcgatg gtctgctggc ccggcagctt gaacttgaag
     1861 ttcggcttga tcgagaacgc cgacgtggca ccgccctggt tgcggacctc cacgccgtcg
     1921 atgacgcggc cgtcgaccac gaggacggcg gggacgtact ccttgctgcc ccggttctcg
     1981 gccagccact gcgcatcggc gtcgctcagg taccagtcga ggacggggag ctgggtgctc
     2041 accgccggcc gctccacgac gaagccgtcg tagcgccgct tgccgtcctt cacgg
//
LOCUS       DATMFS010000539         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_134692, whole genome shotgun sequence.
ACCESSION   DATMFS010000539 DATMFS010000000
VERSION     DATMFS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134692"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VNQ33_10135"
     CDS             complement(<1..467)
                     /locus_tag="VNQ33_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98507.1"
                     /translation="MTYRPNRPRRPASPKKIQALRLAGWRYSSKRSAWVHRAFNGRVG
                     PVFVDPSDVHPRINEAIVIDHRGPRRGSGQPMPDDVIALEPDAVAAVHAPLDDKLVIE
                     VGRTPVPVEIVRTPVPIEVETRVKAPAEVEIEAVTWFVAEVEAQAEVEAQAEV"
     gene            682..2157
                     /locus_tag="VNQ33_10140"
     CDS             682..2157
                     /locus_tag="VNQ33_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ98508.1"
                     /translation="MAHRPDDDVGLPIKWHPVSNGEFVPPPATRLVREATRQSRIALE
                     ANARRIGVSRRQFLLSAMGSATMLAVLSACSSDEARSDGKEPGGTFDIPDEATTEPEA
                     AEAAVGGDGLVFDVQTHLLDFPAAMAAGTPLGFPQSDCGEEDWHDCYRRPAFLDLMFL
                     ESDTSAIVLSAIPFPGDLLSPEVMAETMRIGEELCGDGRIFMQAQTNPSAAPVAQLKE
                     SMAQVAADFPITAWKAYCHAGGPGWFLDDHDPTAPQCGEAFLSQAEALGITRVAIHKG
                     FTMIGGVGPDAATYSDPVDIGPAAKAHPNLDIIVYHSGFDVGPAEGPYDEANDYGVDR
                     LITSMRANGIEAGGNVYGELGSTWRFVMSKPTEAAHVIGKLLKHVGPDNILWGTDSIW
                     YGSPQDQIESFRAFQISDELQERYGYPALTPEVKAKIFGLNAAKLHGLDPKAGACRFD
                     KAELEEVRFRTGRKNTTFGPATATAVKLLQAQGEPWEAGAR"
     gene            complement(2234..>3033)
                     /locus_tag="VNQ33_10145"
     CDS             complement(2234..>3033)
                     /locus_tag="VNQ33_10145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWJ98509.1"
                     /translation="APAGTATIHPGVQTFTNGGQCTANFVFTDETSTYIGQAAHCSST
                     GAATETNGCTSGTLPLGTEVQIGGADHPGTLAYSSWITMQANGETDPDACASNDLALV
                     KIDPADVAKVNPSIPHWGGPTGLNTTGTTGTVYSYGNSSLRQGITLLSPKLGLTSSTE
                     GNGWTHVVNTVTPGIPGDSGSAFLDSEGRALGVLSTLGIGLPGGVVNNVSDLNKALGY
                     LHAHGSGSVASAQLVLGTEAFNGAQLPLDPAHPVDDPVGSLRGLLGL"
BASE COUNT          434 a          997 c         1134 g          468 t
ORIGIN      
        1 tcgacctcgg cttgggcttc gacctcggct tgggcttcga cctcggctac gaaccaggtg
       61 accgcttcga tctcgacctc ggctggggct ttgacccggg tctcgacctc gatcgggacg
      121 ggggtgcgca cgatctcgac ggggacgggg gtgcgcccga cctcgatgac gagcttgtcg
      181 tcgagcggcg cgtggacggc ggcgacggca tcgggctcca gcgcgatcac atcgtcgggc
      241 atgggctgcc ccgaaccgcg gcgcgggccg cggtggtcga tcacgatcgc ctcgttgatc
      301 cgtgggtgga cgtcggaggg atccacgaac accgggccga cgcggccgtt gaaggcgcgg
      361 tgcacccagg ccgagcgctt gctcgagtag cgccagcccg cgaggcggag ggcttggatc
      421 ttcttcggcg aggccggccg gcggggccgg ttcggtcgat acgtcatcac ttcccccatc
      481 ggctgcccga gggcagaccc ttcgtgactt gcgctctttc cccctgatga cacgtgatcg
      541 cagtcgtgcc cgtcccgcat cggtgatggc tgcgtcagag aagagtagga gcgatgtgag
      601 ctcagcgcca ctacctcgtg gtgaacatca ggtggtggat ggtcgagcgc agggtcggga
      661 cgctccgtag ggtgtcggcc catggcccac cggcccgacg acgacgtcgg tctcccgatc
      721 aagtggcatc cggtctccaa cggtgagttc gtgccgcccc cggccacccg gttggttcgc
      781 gaggcgaccc ggcagagccg gatcgcgctg gaggccaacg ctcgacggat cggcgtcagc
      841 cgccgccagt tcctgctctc ggccatgggc tcggcgacga tgctcgcggt cttgtcggcc
      901 tgctccagcg acgaggctcg cagcgacggc aaggagccgg gcggcacctt cgacatccct
      961 gacgaggcga cgaccgagcc ggaggctgcg gaggcggcgg tcggcggcga cggcctcgtg
     1021 ttcgacgtgc agacccacct gctcgacttc cccgcggcga tggcggcggg gacaccactg
     1081 gggttcccgc agtccgattg cggcgaggag gactggcacg actgctaccg ccgccccgcc
     1141 ttcctcgacc tgatgttcct ggagagcgac acgagcgcca tcgtcttgtc ggccatcccg
     1201 ttccccgggg acctgctgag ccccgaggtg atggccgaga ccatgcggat cggcgaggag
     1261 ctctgcggcg atggccgcat cttcatgcag gcgcagacca acccgtcggc tgcgccggtg
     1321 gcccagctga aggagtcgat ggcccaggtc gccgccgact tcccgatcac ggcctggaag
     1381 gcctactgcc atgcgggcgg gccgggctgg ttcctcgacg accacgaccc gacggcaccc
     1441 cagtgcggcg aggcgttcct cagccaggcc gaggcgctcg gcatcacgcg ggtggcgatc
     1501 cacaagggct tcaccatgat cggcggggtc ggtcccgacg ccgccacgta ctccgatccc
     1561 gtcgacatcg ggccggcggc gaaggcccac cccaacctgg acatcatcgt gtaccactcc
     1621 gggttcgacg tcggcccggc cgagggcccc tacgacgagg cgaacgacta cggggtcgat
     1681 cgcctgatca cgtcgatgcg cgccaacggc attgaggcgg gcgggaacgt ctacggcgag
     1741 ctcgggtcga cctggcggtt cgtgatgagc aagcccaccg aggcggccca cgtcatcggc
     1801 aagctgctca agcacgtcgg acccgacaac atcctgtggg gcaccgattc catctggtac
     1861 ggctcgcccc aggaccagat cgagtcgttc cgggcgttcc agatctccga cgagctccaa
     1921 gagcgctacg gctacccggc cctcacgcca gaggtgaagg ccaagatctt cggcctcaac
     1981 gccgccaagc tccacggcct cgacccgaag gcgggggcct gccgcttcga caaggccgag
     2041 ctcgaagagg tgcggttccg gaccggtcgg aagaacacga ccttcggccc cgcgactgcg
     2101 acggccgtca agctcctgca ggcgcagggg gaaccgtggg aggccggggc ccggtgactg
     2161 gcggtggggc cgcggcgccg cccggctccg aagtggggcc gggcggcgtc gaggtgcgtg
     2221 gggagcagcc gggtcagagg ccgaggaggc ctcggagcga gccgaccggg tcgtcgaccg
     2281 ggtgggcggg gtcgagcggg agctgggcgc cgttgaacgc ctcggtgccc aagacgagct
     2341 gggcgctggc gaccgagccg ctgccgtggg cgtggaggta gccgagcgcc ttgttgaggt
     2401 cgctcacgtt gttgaccaca ccgccgggaa ggccgatgcc gagggtcgac aagacgccca
     2461 gcgcccggcc ctcgctgtcg aggaaggcgc tgccggagtc accggggatg cccggggtga
     2521 cggtgttgac cacgtgggtc cagccgttgc cctcggtgct gctggtcagg cccagcttcg
     2581 gcgataggag ggtgatgccc tggcgcaggc tcgagttgcc gtacgagtag acggtgcctg
     2641 tggtgcccgt ggtgttcagc ccggtcgggc cgccccagtg ggggatcgac gggttcacct
     2701 tggccacgtc ggccgggtcg atcttcacga gggccaggtc gttggaggcg caagcgtcgg
     2761 gatcggtctc gccgttggcc tgcatggtga tccacgagct gtaggcgagg gtgccggggt
     2821 ggtcggcgcc gccgatctgg acctcggtgc cgaggggcag ggtgccgctc gtgcagccgt
     2881 tggtctcggt ggccgcaccg gtgctcgagc agtgggcggc ctggccgatg taggtgctcg
     2941 tctcgtcggt gaagacgaag ttggcggtgc actgcccgcc gttggtgaag gtctgcacgc
     3001 cggggtggat ggtggcggtg ccggcggggg cgt
//
LOCUS       DATMFS010000540         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_64082, whole genome shotgun sequence.
ACCESSION   DATMFS010000540 DATMFS010000000
VERSION     DATMFS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64082"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNQ33_10150"
     CDS             <1..492
                     /locus_tag="VNQ33_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWJ98510.1"
                     /translation="VAPLLFFLLFGLIDFGVNLSNEISIRQGVREGARQGSVATFGST
                     ASCGATFTTPGSTNMQKLICLTKSRTDMTASDVSVAIRFDPTSSSYPAAAVGASPPVG
                     NGIIVCAASPMVSPSKLLSPLLNGKYLKSKTTMRIEKGSGVAESITNEADPTGANWSW
                     CTP"
     gene            489..1847
                     /locus_tag="VNQ33_10155"
     CDS             489..1847
                     /locus_tag="VNQ33_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWJ98511.1"
                     /translation="MTGPVSHVRRRGRSRRDERGAVLVVSAIAMVALLAIVAIVIDIG
                     YAKQYRRLSQNSADAAALAAAQDLDGTAAKTLSAVVTAKTWAQKNDPTLTPASWTGCT
                     NADALPYTPDPNNSCISFAADYLTVRVMLPTVTQPVFFGGVVGSKGLAVNATATATKT
                     AGAATATAGPCGLCVIAGKTLQIAGNTAIAVTGGEIQADRLTANANDSNSAITPVPLK
                     WYQSNGSNWGRNVQPAPATFNSRYAQLAAPVPNPFAGKTVDYTGLVTDGSNINHNGGT
                     SPISPNRIYTQNVNINGGTLTLAPGTYYFANTLTLQGGAKLIGDGVTLVFSCRASCAS
                     VGGEPGKFNFGQGTTVEITAPTSGPYAGLAIMFDPDGTQGTPNQLSGTITLNGAVYAK
                     QAGFNMGSSSAVIKAWTIVSGGNFDANNGTLLIDNNAFANATGGGSGAGGSTGGSISL
                     VG"
     gene            complement(1883..>2348)
                     /locus_tag="VNQ33_10160"
     CDS             complement(1883..>2348)
                     /locus_tag="VNQ33_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWJ98512.1"
                     /translation="REPAEIKLDLPLDANLSAGYVPKEELRLEAYRRLAEVTTDAQVA
                     DIRAEWEDRYGPVPEEAARLLDVARLRAEAHRLGVREINVTKGPAFGGPAWTARVSPL
                     VLKASQQIRLQRLFKGAVYKEQVEQLMLPIPKTPDLAGTLVDLLRNLVPPAE"
BASE COUNT          386 a          870 c          774 g          318 t
ORIGIN      
        1 gtggcgccgc tgctgttctt cctgctgttc gggctcatcg acttcggcgt gaacctctcc
       61 aacgagatct ccatccgcca aggcgtccgg gagggcgctc ggcaggggtc ggtcgccacg
      121 ttcgggagca cggcgtcgtg cggggcgacg ttcaccacac cgggttccac gaacatgcag
      181 aagctcatct gcctgaccaa gtcccgaacc gacatgacgg cgtccgacgt ctcggtcgcg
      241 atccgcttcg acccgaccag cagctcgtac ccagccgcag cggtcggcgc ctcgccgccg
      301 gtcggcaacg ggatcatcgt gtgcgcggcc tcccccatgg tcagccccag caagctcctc
      361 agtcccctgc tcaacggcaa gtacctgaag tcgaagacga cgatgcggat cgagaagggc
      421 tcgggcgtcg ccgagtcgat cacgaacgag gccgacccga ccggcgccaa ctggtcgtgg
      481 tgcacgccat gacggggccc gtcagccacg tccgccgccg ggggcggagc cgccgggacg
      541 aacggggtgc cgtcctcgtg gtcagcgcga tcgcgatggt cgccctgctc gcgatcgtgg
      601 ccatcgtcat cgacatcggg tacgccaagc agtaccgccg cctgtcccag aactccgccg
      661 acgccgccgc gctcgccgcc gcgcaggacc tcgacgggac cgcggcgaag accctctccg
      721 ccgtcgtcac ggcgaagacg tgggcgcaga agaacgaccc cacgctcacc cccgcatcct
      781 ggacgggctg cacgaacgcc gatgccctcc cgtacacgcc cgatccgaac aacagctgca
      841 tctcgttcgc tgccgactac ctcacggtcc gcgtgatgct cccgacggtg acccaacccg
      901 tgttcttcgg cggcgtggtc ggctccaagg gcttggccgt gaacgcgacg gccaccgcga
      961 cgaagacggc gggggcggcc accgccaccg ccgggccctg cggcttgtgc gtcatcgcgg
     1021 gcaagaccct ccagatcgcc ggcaacacgg ccatcgcggt gaccggcggc gagatccaag
     1081 ccgaccggct cacggcgaac gccaacgaca gcaacagcgc gatcacgccg gtcccgctca
     1141 agtggtacca gtccaacggc agcaactggg gccgcaacgt gcagcccgcc ccggccacgt
     1201 tcaactcgcg ctacgcccag ctcgccgcgc ccgtgcccaa tccctttgcc ggcaagaccg
     1261 tcgactacac gggcctcgtc accgacgggt cgaacatcaa ccacaacggc ggcaccagcc
     1321 cgatctcgcc gaaccggatc tacacgcaga acgtcaacat caacggcggc accctgacgc
     1381 tcgcgcccgg cacctactac ttcgccaaca cgctgaccct ccagggcggc gccaagctca
     1441 tcggcgacgg cgtcaccctg gtgttctcgt gccgcgcctc gtgcgccagc gtcggcggcg
     1501 agcccggcaa gttcaacttc ggccagggca cgaccgtcga gatcaccgcc ccgacctcag
     1561 gaccctacgc cggcctggcg atcatgttcg accccgacgg aacccagggc acccccaacc
     1621 agctgagcgg gacgatcacc ctgaacggcg cggtctacgc caagcaggcc ggcttcaaca
     1681 tgggctcgtc gagcgccgtg atcaaggcgt ggacgatcgt ctcgggcggc aacttcgacg
     1741 ccaacaacgg cacgctcctg atcgacaaca acgccttcgc caacgcgacc ggcggcggca
     1801 gcggggccgg agggtccacc ggtggttcca tcagcctcgt cggctgaggg tgcccagggg
     1861 gcggcgagct ggccccgtgc gactactcgg cgggcgggac gaggttcctc aacaagtcga
     1921 cgagggtgcc ggcgaggtcg ggggtcttgg ggatggggag catgagctgc tccacctgct
     1981 ccttgtagac agcgcccttg aagaggcgct ggaggcggat ctgctggctg gccttcaaga
     2041 ccagcggcga cacccgggcg gtccacgccg ggccgccgaa cgccgggccc ttggtcacgt
     2101 tgatctcccg cacgccgagg cggtgggcct cggcacggag gcgggcgacg tcgagcaggc
     2161 gggcggcctc ctccggcacc gggccgtagc ggtcctccca ctcggcgcgg atgtcggcca
     2221 cctgggcgtc ggtggtgacc tcggcgaggc ggcggtaggc ctcgagccgc agctcctcct
     2281 tcggcacgta gccggccgag aggttggcgt cgaggggcag gtcgagcttg atctcggccg
     2341 gctcgcgg
//
LOCUS       DATMFS010000541         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_42511, whole genome shotgun sequence.
ACCESSION   DATMFS010000541 DATMFS010000000
VERSION     DATMFS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42511"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VNQ33_10165"
     CDS             <1..659
                     /locus_tag="VNQ33_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98513.1"
                     /translation="ALGGLEADLARCGVEEEQAAAAVAEAEAEVALANGAAFDDAVAA
                     ANEAEAALARSTAREEGARRAIEEFSGEAGVAAMIQAAEERVANGEQRVTAAAAAEQA
                     TAAQLAEVDAAFAAATAAEEQALADAASIDRAELVDDIDWELMSRLAALRSVGLAGSL
                     PLVLDDPFTVLDDDEVTNVLDRLVRLADAVQVIVITDREAAVAWAAQIGSEKALVHTA
                     "
     gene            complement(768..1274)
                     /locus_tag="VNQ33_10170"
     CDS             complement(768..1274)
                     /locus_tag="VNQ33_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWJ98514.1"
                     /translation="MAESSDYVPIVVEIPRGSRNKYEIDHETGEIWLDRRLFSATVYP
                     ADYGFVEHTLGEDGDPLDALVIMEEPTFPGCRVRARPVGVFWMDDDAGPDAKIICVPY
                     GDPRWDHVQDIDQLPMFLTDEIEHFFEVYKQLEPGKFANTKGFEGREKAEAEIEDSLA
                     RAKGTSFA"
     gene            1362..>2068
                     /locus_tag="VNQ33_10175"
     CDS             1362..>2068
                     /locus_tag="VNQ33_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ98515.1"
                     /translation="MEFRRITNLPPYVFTIIDGLKIEARRAGVDVVDLGFGNPDLPSP
                     AIAVEKLSEAAHNARNHRYSSSRGIPKLREAVADLYLRRFGVTLDPEKEIISTIGAKE
                     GFSHLMWVLLQPGDVALVPTPSYPIHIWGPYFAGADARQVPIGDGTDAAGYIDRIIEA
                     WELGWPKPRVVVLSFPHNPTTTVATKEDLQRLVDWARDKDVVLVHDLAYADMTFDGFE
                     PPSIMQCEGAREVAVEL"
BASE COUNT          309 a          739 c          721 g          299 t
ORIGIN      
        1 ccgcgctcgg cggcctggaa gccgacctcg ctcggtgcgg cgtcgaggag gagcaggccg
       61 cagcggcggt cgccgaagcc gaggccgagg tcgccctggc gaacggcgcg gccttcgacg
      121 acgccgtcgc ggccgccaac gaggccgagg cggcgctggc ccgttcgacg gcacgggagg
      181 aaggcgcccg ccgggcgatc gaggagttct ccggcgaggc cggtgtcgcc gccatgatcc
      241 aggccgccga ggagcgggtg gccaacggcg agcaacgggt gaccgccgcc gccgccgccg
      301 aacaggcgac cgccgcccag ttggcggagg tcgacgctgc gttcgccgca gcgacggccg
      361 cggaggagca ggcgctggcg gatgccgcat cgatcgaccg ggccgagctc gtcgacgaca
      421 tcgactggga gctgatgtcc cggctcgccg ccctccgttc ggtgggcttg gccggatcgc
      481 tccccctcgt cctcgacgac ccgttcaccg tgctcgacga cgacgaggtc acgaacgtcc
      541 tcgaccgcct cgtccgcctg gccgacgccg tccaggtcat cgtcatcacc gaccgcgaag
      601 ccgccgtcgc gtgggcggcc cagatcggtt ccgagaaggc cctcgtccac accgcctgac
      661 gcgcgcgacg tgcccggctt ccgcccgccg cctccaacgg ggaggacgtc ggcgctgggt
      721 ggacgacgat ccacccggtt ggcaggcgat ggcggacccg gtgagggcta tgcgaagctg
      781 gtgcccttgg ctctcgccag gctgtcttcg atctcggctt cggccttctc gcggccttcg
      841 aagcccttcg tgttggcgaa cttgccaggc tcgagctgct tgtagacctc gaagaagtgc
      901 tcgatctcgt cggtgaggaa catcgggagc tggtcgatgt cctgcacgtg gtcccagcgg
      961 gggtcgccgt aggggacgca gatgatcttg gcgtcgggac cggcgtcgtc gtccatccag
     1021 aacacgccga cggggcgggc ccgcacccgg caacccggga acgtgggctc ctccatgatc
     1081 acgagggcgt cgagcggatc gccgtcctcg cccagggtgt gctccacgaa gccgtagtcc
     1141 gcggggtaca cggtggcgga gaacagccgc cggtcgagcc agatctcccc ggtctcgtga
     1201 tcgatctcgt acttgttccg gctgccgcgt gggatctcca ccacgatcgg cacgtagtcg
     1261 ctggattcgg ccatccaccc agcatggcag gtggctcgga ggccccgaac ggcgagaacg
     1321 catgtcgaag caggaggggg ccacgtccta acgtgcaacg catggagttc cgccggatca
     1381 cgaacctgcc gccctacgtc ttcaccatca tcgacggcct gaagatcgag gctcggcggg
     1441 ccggggtcga cgtcgtcgac ctcggcttcg gcaacccgga cctcccgtcg ccggccatcg
     1501 ccgtcgagaa gctgtccgag gcggcgcaca acgcccgcaa ccaccgctac tcgtcgagcc
     1561 ggggcatccc gaagctgcgc gaggcggtgg ccgacctcta cctgcgccgg ttcggcgtca
     1621 cgctggatcc cgagaaggag atcatctcca ccatcggcgc caaggagggc ttcagccacc
     1681 tcatgtgggt gctgctccag ccgggcgacg tcgcgctcgt cccgaccccg agctacccca
     1741 tccacatctg gggcccctac ttcgcgggcg ccgacgcgcg ccaggtcccc atcggcgacg
     1801 ggaccgacgc cgcggggtac atcgaccgca tcatcgaggc ctgggagctg ggctggccca
     1861 agccgcgcgt cgtcgtcctg tcgttcccgc acaacccgac gacgacggtc gcgaccaagg
     1921 aggacctcca gcgcctcgtc gactgggcgc gggacaagga cgtcgtgctc gtgcacgacc
     1981 tcgcctacgc ggacatgacg ttcgacgggt tcgagccgcc ctcgatcatg cagtgcgagg
     2041 gcgcgcgcga ggtcgcggtc gagctgta
//
LOCUS       DATMFS010000542         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_42532, whole genome shotgun sequence.
ACCESSION   DATMFS010000542 DATMFS010000000
VERSION     DATMFS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42532"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1153
                     /locus_tag="VNQ33_10180"
     CDS             74..1153
                     /locus_tag="VNQ33_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWJ98516.1"
                     /translation="MRFAFTDDQLAFRDAVADLLAKECPPEVVRAAWPDGTDAHGARK
                     GEGARADADRVAKVWTDLAEMGVLGIGVSEASGGLGLAEDDWVLLAEETGYAALPHPF
                     VETACVVAPLLDDTDDPHGVLADLLDGSRQAGAVPLTSALVPWGDTVTYLVSIDRGRG
                     GEAGWVARRIEGGLAVGDTDERRRTLVDAVDAGRRVVKLGAWQADQGFAMGTIAERAF
                     DRGALGTAAQLVGLGRRLLDLTVAYAIERKQFGVAIGTQQAVKHHLATAAMKLRFAAP
                     VVYAAAWELSTGAPTASRTVSTAKALASDAARFTAAQALQCHGAIGYTVEYDLHLYLK
                     RAEALSRTWGDAAWHRRRVAAALGI"
     gene            1250..2401
                     /locus_tag="VNQ33_10185"
     CDS             1250..2401
                     /locus_tag="VNQ33_10185"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWJ98517.1"
                     /translation="MPEAYIVDAVRTPVGKRGGSLSGVHPADLGAASIKALLERTAID
                     PGAVEDVIFGNVDSVGGQAGDIARTCWLVAGGPEHVPGTTVDRQCGSAQQAVHFAAQA
                     VMAGVNDLVVAGGVQQMSQIPISSAMTCATELGFPDPFTTSPGWVERYGPNEVSQFTG
                     AEMIAEKWELTREEMEDFAIESHTRALKAQADGRFEAEIAPLNGLTSDEGPREPNVEK
                     IRSLRTLVEGGRLTAAVASQISDASASVLIASEQAVKDHGLTPRARIHHMSVRGDDPI
                     FMLTAPIPATKHALDKTGLTMDDIDTVEINEAFASVVLAWAKELHPDMAKVNPNGGAI
                     ALGHPLGATGARLMTTMLNHLEQTGGRYGLQTMCEGGGQANVTIIERLG"
     gene            complement(2435..3088)
                     /locus_tag="VNQ33_10190"
     CDS             complement(2435..3088)
                     /locus_tag="VNQ33_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98518.1"
                     /translation="MTDPTPGPTRDTDPPSPESATVPVPTRRIVTVRNERTLRLGWAA
                     VGIGIVLVVIGAAVGFGSQALQVAAAKGRAKVGNPVTFDADDGTTYAITLIPEPTTGD
                     FVEDRISQLGCEVRHPDGTTESLDPSSAEVRTSNSIGVFATDFEGRGGSTTVLCEWER
                     GDLGSFYAVARTHRATRYIGTGALVAGIVIGIGGVGALVRGYRGRPELQDIGTATDR"
     gene            complement(3085..4077)
                     /locus_tag="VNQ33_10195"
     CDS             complement(3085..4077)
                     /locus_tag="VNQ33_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98519.1"
                     /translation="MSAMALFGGPSADKILAKGSATTGRVTGIRVRSVSDGDSDRRVD
                     EYAVEHAGGTNGIRQVLIPDDVVRLGMEVDLALLGGAGAIDWAATGRRQGFDGTNETY
                     RFKALQEPPRPGIVDEEKPLATARKQGEPVTITVTGLEERSYLGAMAIRLTATVTVEL
                     PGTEPYETEIKGLAVPFYASHLIQVGLVAPGFVVLKRLDKPVIDWAAAANADPGLGRP
                     PARPRNADTDTDAVAGAGADLLDGRSVDEAVAAAAAGGAVHGGIDLATYVAVEAGLQR
                     DRVKPAELDAYAAAKGVPMGTWASASAAWQAAIRTDWRLGAAFGEAFEAERKRR"
     gene            4108..4245
                     /locus_tag="VNQ33_10200"
     CDS             4108..4245
                     /locus_tag="VNQ33_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98520.1"
                     /translation="MCGPGRRDAATRTREEVVAHDARSRAAFPDQRHENVGHGALLAA
                     R"
     gene            4352..4804
                     /locus_tag="VNQ33_10205"
     CDS             4352..4804
                     /locus_tag="VNQ33_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98521.1"
                     /translation="MPMPSDVFRWGMALTGEAMRLPRTAAKAREVVVVLPEQLAELIA
                     ALDRFTELLDTSLGEVRDAVSDVGGRLDKLQVSIDGLAGELSSTAAGLDQALPLVSDV
                     VVRLDERVERMDDLLTQVSGSVVGTINAVPGLRRVTRRNKAAADEPSS"
     gene            complement(4874..5245)
                     /locus_tag="VNQ33_10210"
     CDS             complement(4874..5245)
                     /locus_tag="VNQ33_10210"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ98522.1"
                     /translation="MSIASLKMVTLDSSDARRDAAFWAAVLGWDVAHEQDEYAMLKGP
                     DGAALGFGTLGDHEPPTWPNEHGTKQFHFDLGVDDLDAAATRCVELGATLPDEQPGET
                     WRVLLDPSGHPFCLTSNANWG"
     gene            5302..>5956
                     /locus_tag="VNQ33_10215"
     CDS             5302..>5956
                     /locus_tag="VNQ33_10215"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HWJ98523.1"
                     /translation="MAARTNATGATASTAGILRPDEFARHAELSRSPAGAEVAHWVEN
                     HWALRWNLPEGRSFASQVLPHPTCSLTVELGTHPRPEVPPGEVVVVTGVVTRRFDVDV
                     QGSGRVAGVRFRPGGLAALTGCDASEWTDTVVPARTVLPATTCGVLADPALAEEPASW
                     AGAAEAALLERADGRTDPRYDELLGIVADMLGDRTLVTVAEVGQRHGLSARSLQRLFT
                     "
BASE COUNT          776 a         2152 c         2229 g          799 t
ORIGIN      
        1 ggcaccaacg agatccagcg caacatcgcc gccgagcgcg tcctcggcct gccgaggaag
       61 tagggcgacg acgatgaggt ttgccttcac cgacgaccag ctggccttcc gcgacgcggt
      121 ggccgacctc ttggccaagg agtgcccgcc cgaggtcgtg cgcgccgcgt ggcccgacgg
      181 caccgacgcc cacggtgccc gcaagggcga aggcgcccgt gccgacgccg accgcgtcgc
      241 caaggtgtgg accgatctgg ccgagatggg ggtcctcggc atcggggtgt ccgaggccag
      301 cggcggcctg ggcctggccg aagacgattg ggtcctgctc gccgaggaga ccgggtacgc
      361 ggcgctcccg cacccgttcg tggagaccgc ctgcgtggtg gccccgctgc tcgacgacac
      421 cgacgacccc cacggcgtgc tggccgacct gctcgacggc tcccgccagg cgggcgcggt
      481 gccgctcacc agcgcgctcg tcccgtgggg cgacaccgtg acctacctgg tcagcatcga
      541 ccgcggccgc ggcggcgagg ccggttgggt ggcgcgccgg atcgagggcg gcctggcggt
      601 gggcgacacc gacgagcggc gccgcacgct ggtcgatgcc gtcgatgccg gccgccgggt
      661 cgtgaagctc ggcgcgtggc aggcggacca gggcttcgcc atgggcacca tcgccgagcg
      721 ggcgttcgac cggggcgcgc tcggcaccgc cgcccagctc gtgggcctcg gtcggcgcct
      781 gctcgacctc acggtggcct acgcgatcga gcgcaagcag ttcggcgtcg ccatcgggac
      841 gcagcaggcg gtcaagcacc atctggccac ggcggccatg aagctgcgct tcgcggcccc
      901 ggtcgtctac gccgccgcgt gggagctgag cacgggcgcc cccaccgcca gccgcacggt
      961 gtcgacggcc aaggccctcg cctccgacgc cgcccgcttc acggccgcgc aggccctcca
     1021 gtgccacggt gccatcggct acacggtcga gtacgacctc cacctgtacc tcaagcgggc
     1081 cgaggcgctc agccgcacct ggggcgatgc cgcctggcac cgccgccgcg tcgccgccgc
     1141 cctgggcatc tgaccgggct cggctccgac cgggtcgacg ggtccgacga cgagaacggt
     1201 gccgacgctc gcttggccgg gttttctgac ggggcgttag attcggggca tgcctgaggc
     1261 ctacatcgtc gacgccgtcc gcacgcctgt cgggaagcgg ggtggctcgc tcagcggcgt
     1321 gcacccggcc gacctgggcg ctgcttcgat caaggcgctc ctcgagcgca ccgccatcga
     1381 cccgggagcg gtcgaggacg tcatcttcgg caacgtcgac tcggtcggcg gccaggcggg
     1441 cgacatcgcc cgcacctgct ggctggtggc cggtggcccc gagcacgtgc cgggcaccac
     1501 ggtggaccgc cagtgcggtt cggcccagca ggccgtccac ttcgccgccc aggccgtgat
     1561 ggccggcgtc aacgacctgg tcgtcgccgg tggcgtccag cagatgagcc agatcccgat
     1621 cagctcggcc atgacctgtg ccaccgagct gggcttcccc gacccgttca ccacctcgcc
     1681 cggctgggtc gagcgctacg ggccgaacga ggtcagccag ttcaccggcg ccgagatgat
     1741 cgccgagaag tgggagctga cccgggagga gatggaggac ttcgccatcg agtcccacac
     1801 ccgggcgctc aaggcccagg ccgacggccg cttcgaggcc gagatcgcgc cgctgaacgg
     1861 cctgacctcc gacgagggcc cccgggagcc gaacgtcgag aagatccgct cgctgcgcac
     1921 cctggtggag ggcggccggc tcacggccgc ggtggccagc cagatcagcg acgcctcggc
     1981 gtcggtcctc atcgccagcg agcaggcggt gaaggatcac ggcctcaccc cccgggcccg
     2041 catccaccac atgagcgtcc gcggcgacga cccgatcttc atgctcaccg cgccgatccc
     2101 ggccacgaag cacgcgctcg acaagaccgg cctgaccatg gacgacatcg acacggtcga
     2161 gatcaacgag gcgttcgcct ccgtggtgct ggcgtgggcg aaggagctcc acccggacat
     2221 ggccaaggtc aacccgaacg gcggcgccat cgccctgggc cacccgctcg gcgccaccgg
     2281 cgcccggctg atgaccacga tgctcaacca cctcgagcag accggtggcc gctacggcct
     2341 ccagacgatg tgcgagggcg gcggtcaggc caacgtgacc atcatcgagc gcctgggctg
     2401 accgtcgccg gcacgcgccg acgtgtcgac gggctcagcg atcggtggcg gtgccgatgt
     2461 cttggagctc gggccggccg cggtagcccc ggaccagggc gccgacgccg ccgatgccga
     2521 tcacgatccc ggccaccagc gcgcccgttc cgatgtagcg ggtggcccgg tgggtgcggg
     2581 cgacggcgta gaagctgccg aggtcgccgc gctcccactc gcagaggacg gtggtcgacc
     2641 cgcctcgtcc ctcgaagtcg gtggcgaaca cgccgatcga gttgctggtg cggacctcgg
     2701 ccgagctcgg gtcgagcgac tcggtggtgc cgtcggggtg tcggacctcg catccgagct
     2761 gggagatgcg gtcctcgacg aagtcgccgg tggtcggttc ggggatcagg gtgatcgcgt
     2821 aggtcgtgcc gtcatccgcg tcgaaggtca cggggttgcc gaccttcgcc cgccccttcg
     2881 ccgccgccac ctggagggcc tggctcccga agccgaccgc ggcgccgatc accacgagca
     2941 cgatcccgat cccgaccgcg gcccatccga ggcgcagggt gcgctcgttg cgcacggtga
     3001 cgatgcgccg ggtcggcacc gggacggtgg cggattcggg gctcggcggg tcggtgtctc
     3061 gggtcggtcc gggggtcggg tcggtcatcg gcgcttgcgc tccgcctcga acgcctcgcc
     3121 gaacgcagcg ccgagccgcc agtcggtgcg gatggcggcc tgccaggcgg cgctggccga
     3181 ggcccacgtc cccatcggga cgcctttggc ggcggcgtag gcgtcgagct ccgccggctt
     3241 cacccggtcc cgctggaggc cggcctcgac cgccacgtag gtcgccaggt cgatcccgcc
     3301 gtgcaccgcg ccgccggcgg ccgcggcggc gacggcttcg tcgaccgagc gtccgtccaa
     3361 caggtcggcc cccgcccccg ccacggcgtc ggtgtcggtg tcggcgttgc ggggacgcgc
     3421 cggaggccgg ccgaggcccg gatcggcgtt ggccgccgcc gcccagtcga tcaccggctt
     3481 gtcgaggcgc ttgaggacca cgaaccccgg tgctacgagc ccgacctgga tcaggtggct
     3541 ggcgtagaag ggcacggcca ggcccttgat ctcggtctcg tacggctcgg tgccgggaag
     3601 ctcgacggtc accgtggcag tcaggcggat cgccatggcg ccgaggtagg agcgctcctc
     3661 gagcccggtg accgtgatgg tgacgggctc gccctgcttg cgggcggtgg cgaggggctt
     3721 ctcctcgtcg acgatgccgg gcctgggtgg ctcctgcagc gccttgaacc ggtaggtctc
     3781 gttcgtgccg tcgaagccct ggcgccgtcc ggtggcggcc cagtcgatgg caccggcacc
     3841 gcccagcagg gcgaggtcga cctccatgcc gaggcgcacg acgtcgtcgg ggatgaggac
     3901 ctggcggatg ccgttcgtgc cgccggcgtg ctcgacggcg tactcgtcga cccggcggtc
     3961 cgagtcgcca tcggacacgg agcgcacgcg gatgcccgtg acgcggcctg tggtcgccga
     4021 ccccttcgcc aggatcttgt cggccgacgg cccaccgaac agcgccatgg cggacatgtt
     4081 gccgcgtctt ggctggacgt gcgtccgatg tgcggccccg ggcggcgcga tgctgcgacc
     4141 cggacccgcg aggaggtggt ggcgcacgat gcccgcagcc gcgcggcctt ccccgaccag
     4201 cgccacgaga acgtcggcca cggcgccctc ctcgccgccc gctgaccgct ggaggacgcg
     4261 ggcgcgagcc ggcgaagcgg agcagactgc ggacatggca gcggatgcgg gtgaggacga
     4321 agcagcggca gggacgcggt cgggggcggg ggtgccgatg ccgtcggacg tgttccgctg
     4381 gggcatggcg ctcaccggtg aggcgatgcg cctgccccgc acggcggcga aggctcgcga
     4441 ggtcgtggtc gtgctgcccg agcagctggc cgagctgatc gccgcgctcg atcgcttcac
     4501 cgagctgctc gacaccagcc tcggcgaggt gcgcgacgcg gtgagcgacg tgggaggccg
     4561 gctcgacaag ctccaggtct cgatcgacgg cctggctggc gagctcagct ccacggccgc
     4621 cggcctcgat caggcgcttc cgctcgtgtc cgacgtggtg gtccgcctcg acgagcgggt
     4681 cgagcgcatg gacgacctgc tgacgcaggt gagcggttcg gtggtgggca ccatcaacgc
     4741 cgttcccggc ctccgccgtg tcacccgccg caacaaggcc gccgccgacg agccatcgag
     4801 ctgacggtgg agcgcccctt ccgctggtcg actcggcgag gcgacggccc cgtcgcgtga
     4861 ccagcccggt ggctcagccc cagttggcgt tgctggtgag gcagaagggg tggccgctcg
     4921 ggtccagcag gacgcgccag gtctcgccgg gctgctcgtc ggggagggtg gcgcccagct
     4981 cgacgcagcg ggtggcggcg gcatcgaggt cgtccacgcc gaggtcgaag tggaactgct
     5041 tggtcccgtg ctcgttcggc caggtgggcg gctcgtggtc gccgagggtg ccgaagccga
     5101 gcgcggcgcc gtcggggccc ttgagcatgg cgtactcgtc ttgctcgtgg gcgacgtccc
     5161 agcccagcac ggccgcccag aacgcggcgt cccggcgggc gtcggagctg tcgagggtga
     5221 ccatcttcag cgaggcgatg gacatggggc ggcctttcgt cggcggatcg gaggagggcc
     5281 cagcgtgctt acgctgggcc ggtggccgct aggacaaacg cgacaggcgc gaccgcctcg
     5341 acggcgggga tcctccggcc cgacgagttc gcccgccacg cggagctctc ccggtcgccg
     5401 gcgggggccg aggtggcgca ctgggtggag aaccactggg cgctgcgctg gaacctgccc
     5461 gagggccggt ccttcgccag ccaggtgctc ccgcacccga cctgctcgct gaccgtcgag
     5521 ctcggcaccc acccgaggcc cgaggtcccg ccgggcgagg tggtcgtggt gaccggcgtg
     5581 gtgacccggc gcttcgacgt cgacgtgcag gggtcgggcc gcgtcgcggg cgtgcgcttc
     5641 cggcccggtg ggctcgccgc gctcaccggc tgcgatgcca gcgagtggac cgacaccgtg
     5701 gtcccggccc ggacggtcct cccggcgacg acgtgcgggg tcctggccga cccggccctg
     5761 gccgaagagc cggcctcgtg ggcgggggcc gcggaagccg cactgctcga acgggccgat
     5821 ggccggaccg acccgcgcta cgacgagctg ctcggcatcg tcgccgacat gctcggggac
     5881 cggacgctgg tgaccgtcgc cgaggtgggc cagcgccacg ggctgtcggc ccgcagcctc
     5941 cagcgcctct tcaccc
//
LOCUS       DATMFS010000543         3729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_272260, whole genome shotgun sequence.
ACCESSION   DATMFS010000543 DATMFS010000000
VERSION     DATMFS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272260"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VNQ33_10220"
     CDS             complement(<1..811)
                     /locus_tag="VNQ33_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98524.1"
                     /translation="MTDSAGPSADLRGAGAAPDRVSPTPSPGTRRERLIGFAVVGAIM
                     AATCGELIRAGVSVTRVDLGSSWQLIDVPVLEHDPLGSVWYLHTQPPLYNLVIGGLLR
                     WSPLPPLGSIFGLYLAALCGAAFLFTDVLAKWRVRPWIAGLIAGVVLADPNLLATINI
                     ASYEVPVTFLLMLVLWCFQRYLQRPGWGPLLGLSGALTALVMMRSLFHPAFALVVVGV
                     AVVARRASWRQVTAGLAIPIVLAGGWMLKNEVLFGTPTMSSWLGFNVQRGVV"
     gene            925..1575
                     /locus_tag="VNQ33_10225"
     CDS             925..1575
                     /locus_tag="VNQ33_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ98525.1"
                     /translation="MTSSAPELRLVDVDAPAAGREVEARILDAALVLVARWGVAKTAL
                     GDVAKEAGCSRATLYRAFPGGKQHLFVALATRELLTYRRGILDAIDAADELGDALTRA
                     LVVAARLLHDHDAAQFILEHEPELLLPFLGFHQVDVLYRETATAFGPHLERFLPDASA
                     ERAEWAAEWASRLFITYLFNPHERRDLAVIDDARSLIETFVLPALAPAPTAAPRTT"
     gene            1587..2732
                     /locus_tag="VNQ33_10230"
     CDS             1587..2732
                     /locus_tag="VNQ33_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ98526.1"
                     /translation="MSTNVESSNERLIGRDDINDLEAILSVSASDAEESIRVVKDNAD
                     AIFTWDYEKGSRPALNKLYEKAKVSMWNGETDLPWDTVVDQEQVARDNMALGTFGDID
                     LSHTPFAKWNDDDWANLGMEFQNWSLSQFMHGEQGALICTAKIVETVPWIDAKYYAST
                     QVMDEARHVEVFAKYLDTKLNGHYPLNTHLGLLLDDIIEDSRWDMTYLGMQIMVEGLA
                     LAAFGFMHQMTTEPLLKKLLRYVMSDEARHVAFGVLSLKEYYAELSDAEMLERQEFAF
                     EAAVRMRDRFMQQEIWERLGLKPKEVLPLLLDNPNRLLFQQMLFTKIVPNCKKLGLLD
                     RNDGWLRHKFEELGVIAFEDLTDTSDEYEAFALGAQELSESTSAMTA"
     gene            2901..>3729
                     /gene="dapA"
                     /locus_tag="VNQ33_10235"
     CDS             2901..>3729
                     /gene="dapA"
                     /locus_tag="VNQ33_10235"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWJ98527.1"
                     /translation="MGRFGTVITAMATPFAADGSFDAKAAGELAKWLVAHGNDALVLA
                     GTTGESAVLTDPERIELFRVVRDAVDVPLIAGSTTNDTAHSVEMTQAATEAGMDAVLA
                     VVPYYNKPSQQGLALHFRAVAEATDKPVMLYDVPGRTGRPLETGTIVELAQALPNIVA
                     LKDAGGDPSRAGRLLAAAPEGFEVYSGDEPLSLGFAAYGAVGVVGVATHWSAELQGEM
                     FAAFAKGDLVTARQINDQLAASFTYMNSDTCVFSQSVKVAMGVLGVAVGECRLPLGPA
                     "
BASE COUNT          615 a         1338 c         1256 g          520 t
ORIGIN      
        1 ccacgacccc gcgctggacg ttgaagccga gccagctgga catggtcggc gtgccgaaga
       61 gcacctcgtt cttgagcatc cacccgcctg cgagcacgat ggggatcgcg aggccggcgg
      121 tgacctggcg ccagctggcg cggcgggcga ccacggcgac gccgacgacc accagggcga
      181 aggcggggtg gaacaggctc cgcatcatga ccagtgcggt cagcgcgccg ctcagcccga
      241 gcaacggacc ccagccgggc cgttgcaggt agcgctggaa gcaccagagg acgagcatga
      301 ggaggaaggt gacggggacc tcgtagctcg cgatgttgat cgtggcgagc aggttgggat
      361 cggcgagcac cacgccggcg atgagcccgg cgatccaggg ccggacgcgc cacttcgcga
      421 gcacgtccgt gaacaagaag gccgcgccgc acagcgcagc gaggtagagc ccgaagatgg
      481 atccgagggg cgggaggggc gaccatcgga ggagcccgcc gatcacgagg ttgtagagcg
      541 ggggttgggt gtggaggtac cagacgcttc cgagcgggtc gtgctcgagc accggcacgt
      601 cgatgagctg ccagctcgat cccaggtcga cacgggtcac cgagacccca gcccggatca
      661 gctccccgca cgtcgccgcc atgatggcgc cgaccacggc gaagccgatc agccgttcgc
      721 ggcgcgtccc gggtgatggc gtgggactga cgcggtctgg ggccgcacca gccccacgca
      781 ggtctgcgct gggtcccgcc gaatcggtca tccggtggag tctcgcaccc ccgctcccgc
      841 cgggcgggac cgcacgctgc tgtggcgcaa gtcacggatg cgacatgttg acgatgagac
      901 actttccttc ttagtgtctc acccatgacc tcatcggctc cggagctgcg cctcgtcgac
      961 gtcgacgcgc cagcggccgg gcgtgaggtc gaggcccgca tcctcgacgc ggccctggtg
     1021 ctcgtggccc gctggggcgt ggccaagacg gcgctgggcg acgtcgccaa ggaggccggc
     1081 tgctcccggg ccaccctgta ccgggcgttc cccggtggca agcagcacct cttcgtcgcc
     1141 ctcgccaccc gcgagctcct gacgtaccgc cgggggatcc tcgacgcgat cgacgcggcc
     1201 gacgagctgg gcgacgccct gacccgggca ctggtcgtcg ccgcccggct gctgcacgac
     1261 cacgacgccg cccagttcat cctcgagcac gagcccgagc tgctcctgcc gttcctgggc
     1321 ttccaccagg tcgacgtgct gtaccgggag acggccacgg ccttcggccc ccacctcgag
     1381 cgcttcttgc ccgatgcgtc ggcggaacga gccgagtggg cggccgagtg ggcctcccgg
     1441 ctgttcatca cctacctgtt caacccccac gagcgccgcg acctcgcggt gatcgacgac
     1501 gcccgcagcc tgatcgagac cttcgtcctg ccggcgctcg cgccggcacc caccgcagcc
     1561 ccccgcacca cctgagagga accaccatgt ccaccaacgt cgagtcttcc aacgagcgcc
     1621 tcatcggccg agacgacatc aacgacctgg aggcgatcct ctccgtgtcc gcctccgatg
     1681 ccgaggagtc gatccgcgtc gtcaaggaca acgccgatgc catcttcacg tgggactacg
     1741 agaagggctc ccgtccggcg ctgaacaagc tctacgagaa ggccaaggtc tccatgtgga
     1801 acggcgagac cgacctcccg tgggacaccg tggtggacca ggagcaggtg gcccgggaca
     1861 acatggcgct gggcaccttc ggcgacatcg acctctcgca caccccgttc gccaagtgga
     1921 acgacgacga ctgggcgaac ctcgggatgg agttccagaa ctggagcctc agccagttca
     1981 tgcacggcga gcagggtgcg ctcatctgca cggcgaagat cgtcgagacg gtcccgtgga
     2041 tcgacgccaa gtactacgcg tccacccagg tgatggacga ggcccgccac gtggaggtgt
     2101 tcgccaagta cctcgacacc aagctcaacg gccactaccc gctcaacacc cacctgggcc
     2161 tgctcctcga cgacatcatc gaggactcgc gctgggacat gacctacctg ggcatgcaga
     2221 tcatggtgga gggcctcgcc ctcgccgcct tcggcttcat gcaccagatg accaccgagc
     2281 cgctgctcaa gaagctgctc cgctacgtga tgagcgacga ggcccgccac gtggccttcg
     2341 gcgtgctgtc gctgaaggag tactacgccg agctgtccga tgccgagatg ctcgagcgcc
     2401 aggagttcgc cttcgaggcc gccgtgcgca tgcgcgaccg cttcatgcaa caggagatct
     2461 gggagcgcct gggcctcaag cccaaggagg tcctcccgct cctgctcgac aacccgaacc
     2521 gcctgctctt ccagcagatg ctgttcacca agatcgtgcc gaactgcaag aagctcggcc
     2581 tgctcgaccg caacgacggc tggctgcgcc acaagttcga ggagctgggc gtgatcgcgt
     2641 tcgaggacct caccgacacg agcgacgagt acgaggcgtt cgccctcggc gcccaggagc
     2701 tctcggagtc cacctcggcc atgacggcct gaggagcacc ggcaggggaa cctggctggc
     2761 gctcgtgcgc cagccgccgg accgtggggt ggccgtcgtg ccaccccacg gccgcgtccg
     2821 gggccgggac cgttgcgggg ccaccggcgg cggggccggt agggtcggag catgcgtgca
     2881 accaccgcag gtcagagctg atgggccggt tcgggaccgt catcaccgcc atggcgacgc
     2941 cgttcgccgc ggacgggtcc ttcgatgcga aggccgcggg cgagctggcg aagtggctcg
     3001 tggcccacgg caacgacgcc ctcgtgctcg ccggcaccac gggggagagc gccgtgctca
     3061 ccgacccgga gcgcatcgag ctgttccggg tggtccgcga cgccgtcgac gtaccgctca
     3121 tcgccggctc caccaccaac gacaccgccc acagcgtcga gatgacccag gccgccaccg
     3181 aggccggcat ggatgccgtc ctcgccgtgg tcccgtacta caacaagccg tcgcagcagg
     3241 gcctggccct ccacttccgg gccgtggccg aggccaccga caagcccgtg atgctctacg
     3301 acgtgccggg ccgcaccggc cggccgctgg agacggggac catcgtcgag ctggcccagg
     3361 cgctgcccaa catcgtggcg ctcaaagacg ccggcggcga cccgtcccgt gcgggccgcc
     3421 tcctggcggc agcacccgag ggcttcgagg tctacagcgg cgacgagccg ctcagcctcg
     3481 gcttcgccgc ctacggcgcc gtcggcgtgg tgggcgtggc cacccactgg tcggccgagc
     3541 tgcagggcga gatgttcgcc gccttcgcca agggcgatct cgtcaccgcc cgccagatca
     3601 acgaccagct ggcggccagc ttcacctaca tgaacagcga tacctgcgtc ttctcgcagt
     3661 ccgtcaaggt cgccatgggc gtgctcgggg tcgcggtggg cgagtgccgc ctcccgctgg
     3721 gcccggccc
//
LOCUS       DATMFS010000544         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_88565, whole genome shotgun sequence.
ACCESSION   DATMFS010000544 DATMFS010000000
VERSION     DATMFS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88565"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VNQ33_10240"
     CDS             <1..330
                     /locus_tag="VNQ33_10240"
                     /inference="COORDINATES: protein motif:HMM:NF044313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98528.1"
                     /translation="RAVGARLVHRDPPDDVRPGPGWVVRQSDLDLFGHVNNAVFWAVA
                     EEAWPVDDLVLPWTAEVEHREGLEPHQAAEVALGRDRLWIFGDGVVAATIVSDRVPSP
                     GPSAPSA"
     gene            complement(284..775)
                     /locus_tag="VNQ33_10245"
     CDS             complement(284..775)
                     /locus_tag="VNQ33_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HWJ98529.1"
                     /translation="MPHGLLPIAAADAAPGLTDPQAVVEAFLAALMASDLEAALELVD
                     DDLVYVNVGLPTIHGRAQLAKAFAAMERPTISFEAYLHAISADGPVVLTERTDVIVWG
                     RFRAQFWVCGRFDVHDGRITLWRDAFDFVDIVRGNVRGLVGMALPRFKPRAPKDRDSA
                     PGR"
     gene            916..1428
                     /locus_tag="VNQ33_10250"
     CDS             916..1428
                     /locus_tag="VNQ33_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ98530.1"
                     /translation="MAHTRPTHGADIDRAEEWDDLRQRFLRPAGERPPSSARGVHHVA
                     LLSNDVETTIGFYQGLLEFPLTTLFENRDYRGSTHFFFDVGNGNTLAFFDMPGLDLGP
                     YQEVLGGLHHLCISVEPGRWAHLKAKLDDAGIPYAHIDEVSVYFDGPDGERLELIADP
                     LGEMYGMQVL"
     gene            1430..2125
                     /locus_tag="VNQ33_10255"
     CDS             1430..2125
                     /locus_tag="VNQ33_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWJ98531.1"
                     /translation="MDLVEALRTTGAVREFEDRPVDREVMARVLDTARFAPNGGNQQS
                     WHVVLVEDPATRSALRALYVPGWLEYLEMRKAGVQPWAPVGDRDAEAAAVARAHQLAG
                     GEAAAAAGGFAAAFDQVPVMAVILADLRLLAATDRDLGRYTLVGGASVYPFVWSILLA
                     ARAEGLGGVVTTMTTTREPEVRDLLRVPDEFALAAVLALGYPVKRPQNLRRDPVESFA
                     TVDAFDGPPVVSP"
     gene            complement(2115..3185)
                     /locus_tag="VNQ33_10260"
     CDS             complement(2115..3185)
                     /locus_tag="VNQ33_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98532.1"
                     /translation="MAAALAASSLVAVGADAAVATPAAPAVDPATVPGPPTIGTASAG
                     HGQVTLTWTAPASDGGSAITSYRITPMIGATAQPEVISRSAGTTKVVTGLSNGVAYTF
                     TVAATNDIGTGPDSLASNLVIPTSPVTPYEPFTSWTAFVDRQFLDITAKPPTSAQRTS
                     WVSQLNLGTKQKGDLVHGLRRGDENLKNVDPVVRIYRAFLGRAPDAGGLKFWINRKRV
                     VAPKRTWNVTQIATDFTNSSEFKRKYGSLTNRQFVNRIYVDVLGRTSDPSGVDYWTKT
                     LDTKKKTKAQVMVGFSESNEYKTKQAQNTDVTVAYVFLLGRAPTVGEAADWVARQKAG
                     TAHTVLLNEILNSAAYSKRITG"
     gene            3305..>3577
                     /locus_tag="VNQ33_10265"
     CDS             3305..>3577
                     /locus_tag="VNQ33_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWJ98533.1"
                     /translation="MGIEITESSQAQVGIVTVRRALPRRQRRTVGAWCFADHLGPAEA
                     TPTRGLDVGPHPHCGLQTVTWLTQGEVLHHDSLGTEQLIAPGQLNLM"
BASE COUNT          495 a         1307 c         1258 g          517 t
ORIGIN      
        1 cgcgccgtcg gcgcccgcct cgtgcaccgc gacccgcccg acgacgtccg ccccggaccg
       61 ggctgggtcg tccgccagag cgacctcgac ctgttcggcc acgtcaacaa cgccgtcttc
      121 tgggccgtgg ccgaggaggc ctggccggtc gacgacctcg tcctgccgtg gaccgccgag
      181 gtcgagcacc gcgaggggct ggaaccgcac caggcggcgg aggtcgcgct cggccgcgac
      241 cgcctgtgga tcttcggcga cggcgtcgtg gccgccacga tcgtcagcga ccgggtgccg
      301 agtcccggtc cttcggcgcc ctcggcttga acctcggaag ggccatcccg accaggcccc
      361 gcacgttgcc gcgcacgatg tcgacgaagt cgaacgcgtc gcgccagagc gtgatcctcc
      421 cgtcgtggac gtcgaagcgg ccgcacaccc agaactgggc gcggaagcgg ccccagacga
      481 tgacgtcggt gcgctcggtg agcaccaccg gcccgtcggc ggagatggcg tggaggtacg
      541 cctcgaacga gatggtcggc cgctccatcg ccgcgaacgc cttggccagc tgcgcccggc
      601 cgtggatcgt gggcagcccg acgttcacgt agacgaggtc gtcgtcgacc agctccagcg
      661 ccgcctccag gtccgaagcc atgagggcgg cgaggaacgc ctcgaccacg gcttgggggt
      721 cggtgagccc gggggcggcg tcggcggcag cgatggggag gagtccgtgc ggcatggcgc
      781 cgatgctacg gccggccgcc ggctgcgggc cgaaggtcca gctgccgttc tcggcgggcg
      841 cgagcagcac cggcgaggag gacggcgatc cgccctagcc tcaccggcac caggacagcg
      901 cgaggggcga gcgacgtggc acacaccaga ccgacccatg gggccgacat cgaccgagcc
      961 gaggagtggg acgacctccg ccagcgcttc ctccgcccgg cgggcgagcg gccgccatcg
     1021 tcggctcggg gcgtgcacca cgtggccctg ctgtcgaacg acgtcgagac gacgatcggc
     1081 ttctaccagg ggctcctcga gttcccgctc acgacgctgt tcgagaaccg ggactaccgg
     1141 ggatcgacgc acttcttctt cgacgtcggg aacggcaaca ccctcgcctt cttcgacatg
     1201 ccgggcctgg acctcgggcc gtaccaggaa gtgctcggcg gcctgcacca cctgtgcatc
     1261 tccgtcgaac ccgggcggtg ggcgcacctc aaggccaagc tcgacgatgc cggcatcccc
     1321 tacgcccaca tcgacgaggt gtcggtctac ttcgacgggc ccgacggcga gcggctcgag
     1381 ctgatcgccg acccgctcgg cgagatgtac gggatgcagg tgctctgacg tggacctggt
     1441 cgaggccctg cgcaccaccg gcgccgtccg cgagttcgag gaccgtcccg tcgatcggga
     1501 ggtgatggcc cgagtgctcg acacggcgcg cttcgcgccg aacggcggca accagcagtc
     1561 gtggcacgtc gtgctggtcg aggaccctgc caccaggtcc gccctgcgcg ccctctacgt
     1621 gcccggctgg ctggagtacc tggagatgcg caaggccggc gtccagccgt gggcacccgt
     1681 cggcgaccgg gacgcggagg cagccgccgt cgcccgggcc caccagctgg ctggcggcga
     1741 ggcggcggcc gcggccggcg ggttcgccgc cgcgttcgac caggtgccgg tgatggccgt
     1801 gatcctcgcc gacctccgcc tgctcgccgc caccgaccgc gacctcggcc gctacacgct
     1861 ggtgggcggc gcgtcggtgt acccgttcgt gtggagcatc ctgctggccg cccgcgccga
     1921 ggggctgggc ggcgtggtca ccaccatgac gaccacccgc gagcccgagg tgcgcgacct
     1981 gctccgcgtg cccgacgagt tcgccctggc cgcggtgctc gccctcggct acccggtgaa
     2041 gcggcctcag aacctgcgcc gcgaccccgt cgagtcgttc gccaccgtcg acgccttcga
     2101 cggaccgccc gtcgtcagcc cgtgatgcgc ttcgagtagg ccgcgctgtt caggatctcg
     2161 ttcaacaaga cggtgtgggc ggtgccggcc ttctgccgag ccacccagtc ggcggcctcg
     2221 cccaccgtcg gcgcccgacc gagcaggaag acgtacgcca ccgtcacgtc ggtgttctgc
     2281 gcctgcttgg tcttgtactc gttcgactcg gagaagccga ccatgacctg cgccttggtc
     2341 ttcttcttcg tgtcgagcgt cttggtccag tagtcgacgc cggacggatc tgaggtgcgg
     2401 ccgaggacgt cgacgtagat ccggttcacg aactggcggt tggtgagcga gccgtacttc
     2461 ctcttgaact cgctgctgtt cgtgaagtcc gtggcgatct gcgtcacgtt ccacgtccgc
     2521 ttcggggcga ccacgcgctt gcggttgatc cagaacttca ggccgccggc atcgggagcc
     2581 cggccgagga acgcccggta gatgcgcacc accgggtcga cgttcttgag gttctcgtcg
     2641 ccccgacgca ggccgtgcac gaggtcgccc ttctgcttcg tgcccaggtt cagctgcgag
     2701 acccagctcg tccgctgggc cgaggtcggg ggcttcgcgg tgatgtccag gaactgccgg
     2761 tcgacgaacg cggtccagga cgtgaagggt tcgtagggcg tgacgggcga ggtcgggatg
     2821 accaggttcg aggccagcga gtcgggaccg gtgccgatgt cgttggtcgc cgcgaccgtg
     2881 aacgtgtagg cgaccccgtt gctcagaccg gtcacgacct tggtcgtgcc ggcgctccgc
     2941 gagatgacct cgggttgggc cgtggcgccg atcatcggcg tgatccggta gctggtgatg
     3001 gccgaacccc cgtccgaagc cggcgccgtc cacgtgagcg tcacctggcc gtgcccggcg
     3061 gaggccgttc cgatcgtcgg cggccccggc accgtcgccg ggtcgaccgc cggtgcggcg
     3121 ggcgtcgcca ccgccgcgtc ggcacccacc gccacgagcg acgatgccgc cagcgcggcc
     3181 gccacagcca gacctcgaac tcgacccatc cctgccctcc caggctcgtt cccctcggga
     3241 cacggtaggc gcgcgtcggg ctggcatcct gaacgggacc gcgccggacc ggaggaccac
     3301 gcccatgggc atcgagatca cggagagctc gcaggcgcag gtgggcatcg tgaccgtccg
     3361 ccgggcgctc ccccggcggc agcgccgcac ggtcggggcc tggtgcttcg ccgaccacct
     3421 gggcccggcc gaggccaccc ccacccgtgg actcgacgtc ggcccgcacc cgcactgcgg
     3481 cctccagacc gtgacgtggc tcacgcaggg cgaggtcctc caccacgaca gcctcggcac
     3541 cgaacagctc atcgcccccg gccagctcaa cctcatg
//
LOCUS       DATMFS010000545         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_298731, whole genome shotgun sequence.
ACCESSION   DATMFS010000545 DATMFS010000000
VERSION     DATMFS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298731"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VNQ33_10270"
     CDS             <1..880
                     /locus_tag="VNQ33_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWJ98534.1"
                     /translation="EEHIRKTSGSAAPPPAPAPPAPAPPAPVPPAPLAPAVPAPVVPA
                     PLPSRAPTPTGVDTTVPLNKIRKITAEHMVRSLGVSAHVYVSTEVDYESIDRVRTVHK
                     AAFKAEEGVSLTYLPFVARALVDALADFPNVNASMGTESELVVDGSVNLGIAVDLDHE
                     GLIVPVVHEAQDRRLRALARDVADLASRAKSRKLAADDITGGTVTITNVGSTGSAFLL
                     PIINQPQVMILSTDGVARRPVVVTDRSGAESIAIHSVGNLTLGWDHRAFDGAYAAQFL
                     AKIKEILETRDWETEL"
     gene            885..2642
                     /gene="lipA"
                     /locus_tag="VNQ33_10275"
     CDS             885..2642
                     /gene="lipA"
                     /locus_tag="VNQ33_10275"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWJ98535.1"
                     /translation="MAGPGVAGARPLHVRWLGRVRYQDALALQHGIHRSSPDDHLLLL
                     EHPHTYTLGVNADRSHVLHDPASVGAELVQSDRGGDVTYHGPGHLVGYPVLHLPGKNG
                     GTKPDTKAYVASVEQLIIDSLGDLGLEADRLPGYPGVWVGVGTDDPRKICAIGIRVDK
                     GKSLHGFGLNVDPDMDMFTHIVPCGISDKAVTSLRAEGVEASMAEVVDVVVARAAQRW
                     GAGGHDRHDVVWRHRDSDLAPFSLGAGPGQLAGKVAGPSRGREPVAVDQQAAGEGTSV
                     RLLGRLAEAGVPQGLAIGERKPEWMRAKARFGPEYLEIKQTMRELDLVTVCEEAGCPN
                     ISECWGDGTATFMINGERCTRACGFCLVDTRHPQAPDPGEPERVAEAVERMGLKYAVL
                     TTVARDDLADGGATQFAETITAVRRRTPGVQVECLISDCKGDPASLDVIFEARPEVLN
                     HNIESVARLQRAVRPSAGYARSLSVLARAKAAGLATKSSIIVGMGETFDELVGVLADL
                     RAVGVDIVTIGQYLRPTTNHLPVARWVTPEEFAELKRIGEAMGIDHVESGPLTRSSYH
                     ARQAADAADVETVVPVSLG"
     gene            2658..3203
                     /locus_tag="VNQ33_10280"
     CDS             2658..3203
                     /locus_tag="VNQ33_10280"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ98536.1"
                     /translation="MGRSFDAIPDHLVSWMEEQPVYFVASAPLSAEGHVNVSPKGGDS
                     FRVIGPNAVAYLDFTGSGAETIAHTRENGRLTVMFASFGPKPNIVRLFGAGRPVLPGD
                     EGFEELAARFPPNPGTRSVITLDVAKVTTSCGYSIPLMEVVGPRSTLDDWAERKGPDG
                     LADYWLEKNQTSIDGLPALAG"
     gene            3271..>3831
                     /locus_tag="VNQ33_10285"
     CDS             3271..>3831
                     /locus_tag="VNQ33_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWJ98537.1"
                     /translation="MAERDVTVLFEGGRFFEGPRWRDGTWWVSDFYRHTVSRIATDGA
                     ETVVVEVEQQPSGLGWLPDGSMVISSMKDHKVLRYADGELSVLADLEGMVAGHLNDLV
                     TDANGHTFVGSFGFDLMGGGQPAAAPLIRVDPDGTTSVAAEDLYFPNGAVITPDGATL
                     IVGETMGNRYTAFDLGADGSLSNRRVW"
BASE COUNT          552 a         1448 c         1311 g          520 t
ORIGIN      
        1 cgaggagcac atccgcaaga cctccggcag cgctgcccct ccgcctgccc ctgcgccgcc
       61 tgcccctgcg ccgcctgctc cggtgccgcc cgccccgctc gccccggctg tgcccgcccc
      121 ggtggtgccg gcgccgctgc cgagccgagc acccacgccc accggcgtcg acaccaccgt
      181 gccgctgaac aagatccgca agatcacggc cgagcacatg gtccgctcgc tcggcgtgtc
      241 cgcccacgtg tacgtcagca ccgaggtcga ctacgagtcg atcgaccggg tgcgcaccgt
      301 ccacaaggcg gcgttcaagg cggaggaggg cgtctcgctc acctacctcc cgttcgtcgc
      361 ccgggccctg gtcgatgccc tggccgactt cccgaacgtc aacgcctcga tgggcaccga
      421 gagcgagctc gtcgtcgacg gcagcgtcaa cctcggcatc gccgtcgacc tcgaccacga
      481 ggggctgatc gtgcccgtcg tgcacgaggc ccaggaccgc cgcctgcgtg cgctcgcccg
      541 tgacgtggcc gacctggcct cccgggccaa gagccgcaag ctcgccgccg acgacatcac
      601 cggcggcacg gtcaccatca ccaacgtggg cagcaccggc agcgccttcc tgctccccat
      661 catcaaccag ccgcaggtga tgatcctctc caccgacggc gtcgcccgcc gtccggtggt
      721 cgtcaccgac cgcagcgggg ccgagagcat cgccatccac tcggtcggca acctcaccct
      781 cggctgggac caccgtgcgt tcgacggcgc ctacgccgcc cagttcctcg ccaagatcaa
      841 ggagatcctg gagacccggg actgggagac cgagctctaa gacgatggcc ggccccggcg
      901 tggcgggcgc ccgcccgctc cacgtccggt ggctcgggcg cgtccgctac caggacgcgc
      961 tggccctcca gcacggcatc caccgctcgt cgcccgacga ccacctcctc ctgctcgagc
     1021 acccgcacac gtacacgctc ggggtcaacg ccgaccgctc ccacgtgctc cacgacccgg
     1081 cgtcggtcgg cgccgagctg gtgcagagcg accgcggcgg cgacgtcacc taccacgggc
     1141 ccgggcacct cgtcggctac ccggtcctgc acctgcccgg gaagaacggc ggcaccaagc
     1201 ccgacaccaa ggcgtacgtc gcctcggtgg agcagctgat catcgactcg ctcggcgacc
     1261 tcgggctcga ggccgaccgc ctgcccggct accccggcgt gtgggtcggc gtcggcacgg
     1321 acgacccccg caagatctgc gccatcggca tccgggtcga caagggcaag agcctccacg
     1381 ggttcggcct caacgtggac ccggacatgg acatgttcac ccacatcgtc ccgtgcggca
     1441 tctccgacaa ggcggtcacg tcgctgcgtg cggagggcgt tgaggcctcg atggccgagg
     1501 tcgtcgacgt ggtggtcgcc cgggcggccc agcggtgggg cgccggcggc cacgaccgcc
     1561 acgacgtcgt ctggcgccac cgcgacagcg atctggcccc gttctccctc ggtgccggac
     1621 ccggccagct cgctggcaag gtcgccggtc cgtcacgggg gcgcgagccg gtggcggtcg
     1681 accagcaggc ggcgggggag ggcacgtcgg tccggctgct cggccgcttg gccgaggcgg
     1741 gggtgcccca gggcctcgcc atcggcgagc gcaagcccga gtggatgcgg gccaaggccc
     1801 ggttcgggcc ggagtacctc gagatcaagc agacgatgcg ggagctcgac ctcgtcaccg
     1861 tgtgcgagga agccggctgc ccgaacatct ccgagtgctg gggcgacggc acggccacgt
     1921 tcatgatcaa cggcgagcgc tgcacccggg cgtgcggctt ctgcctggtc gacacccgcc
     1981 acccgcaggc ccccgatccc ggtgagcccg agcgggtggc cgaggccgtc gagcggatgg
     2041 gcctcaagta cgccgtgctc accaccgtcg cccgcgacga cctcgccgat ggcggcgcca
     2101 cgcagttcgc cgagaccatc accgccgtcc ggcgccgcac ccctggcgtc caggtcgagt
     2161 gcctcatctc ggactgcaag ggcgacccgg ccagcctcga cgtgatcttc gaggcccggc
     2221 ccgaggtcct caaccacaac atcgagtcgg tcgcccggct ccaacgggcc gtgcgcccgt
     2281 cggccggcta cgcccgcagc ctgtcggtcc tggcccgagc caaggccgcc ggtctggcca
     2341 ccaagagcag catcatcgtc ggcatgggcg agacgttcga cgagctggtc ggcgtgctgg
     2401 cggacctgcg ggcggtcggg gtcgacatcg tcaccatcgg ccagtacctg cggcccacca
     2461 ccaaccacct gccggtggcc cggtgggtga cccccgagga gttcgccgag ctcaagcgca
     2521 tcggcgaggc catgggcatc gaccacgtcg agtccggtcc gctcacccgc agcagctacc
     2581 acgcccgcca ggcggccgac gccgccgacg tcgaaaccgt cgtgcccgtc tccctcggct
     2641 gacggcagac tcggcccatg ggccgcagct tcgatgccat ccccgaccac ctcgtctcgt
     2701 ggatggagga gcagccggtc tacttcgtgg cctcggcccc gctcagcgcc gagggccacg
     2761 tcaacgtgtc acccaagggc ggcgactcct tccgggtgat cggaccgaac gcggtggcct
     2821 acctggactt caccgggagc ggtgccgaga cgatcgccca cacccgggag aacggccggc
     2881 tgacggtcat gttcgcctcg ttcgggccca agccgaacat cgtccggctc ttcggcgcgg
     2941 gccgtccggt gctgcccggc gacgaggggt tcgaggagct ggcggcccgg ttcccgccca
     3001 atccggggac gcgctcggtg atcacgctcg acgtcgccaa ggtcacgacg tcgtgcggct
     3061 acagcatccc gctgatggag gtcgtcggcc ctcgctcgac gctcgacgac tgggccgagc
     3121 gcaagggccc cgacggcctg gccgactact ggctcgagaa gaaccagacc tcgatcgacg
     3181 gcctccccgc cctcgcgggg tagctcaggc ctcgtggtgg ggcgaggttg cgccgagcgg
     3241 gcatcgcggg ccgggggtac gttcggccgc atggctgaac gggatgtgac cgtgctgttc
     3301 gagggtggga ggttcttcga ggggccgcgc tggcgcgacg gcacgtggtg ggtctcggac
     3361 ttctaccggc acaccgtgtc gcgcatcgcc accgacgggg ccgagacggt cgtggtggag
     3421 gtcgagcagc agccgtcggg cctgggctgg ctgcccgacg ggtcgatggt gatctcgtcg
     3481 atgaaggacc acaaggtcct ccgctacgcc gacggcgagc tgtcggtgct ggccgatctc
     3541 gagggcatgg tcgccggtca cctcaacgac ctggtgaccg atgccaacgg ccacacgttc
     3601 gtcggcagct tcgggttcga cctgatgggc ggcggccagc cggcggcggc cccgctgatc
     3661 cgggtcgacc ccgacggcac cacgtcggtg gcggccgagg acctgtactt cccgaacggc
     3721 gccgtcatca cgcccgacgg ggcgacgctg atcgtgggcg agacgatggg caaccgctac
     3781 accgccttcg acctcggcgc cgacggcagc ctgtccaacc ggcgggtgtg g
//
LOCUS       DATMFS010000546         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_298739, whole genome shotgun sequence.
ACCESSION   DATMFS010000546 DATMFS010000000
VERSION     DATMFS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298739"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VNQ33_10290"
     CDS             <1..558
                     /locus_tag="VNQ33_10290"
                     /inference="COORDINATES: protein motif:HMM:NF021610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HWJ98538.1"
                     /translation="ESVAEFIDIDLDESSPRLPSAADLEALPLLISAALPIGLDVTGC
                     ATIAGNSTSLQQAVESCEWDVEIRGVEPAAAAGAVDRVLAADDLTITRQRKGKDVTDD
                     VRPYVLALAVVGSTDGGTRVHAHLATQPRGLRVSELLALLDPTWDEGLVRRTAQWTLI
                     DGARHEPLEGPVEATSTSHATARAS"
     gene            574..2643
                     /locus_tag="VNQ33_10295"
     CDS             574..2643
                     /locus_tag="VNQ33_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWJ98539.1"
                     /translation="MTDTDAGDLPTPTPASTDASGAPKADPKLIARADPAPAAEPKPA
                     GGSADAGGSDGASGGETAEGGPGVEAKPRRRGSRGGRNRNRPRPEGSTDDPADGAPPE
                     AGDRPPRSGSAKQGPKGAGDRQPELPDRHRENKPSAEAAEAALVRKPQIGDSRPAPAD
                     GAGDAAATGGGAEGGTGTSSSAKRRRRRGGRGRGGAGGGGGQSGKQGGGNPGGGQRSG
                     NQRKGGGRSGGEPVTAITDDAPVDLDEKTLNKRRGRERKGRPVGRYLMAVHVKPEATQ
                     IAVLEGKALIEHYVSRPADDTLQIHGNIYLARVQNVLPGMEAAFVDIGTPKNAVLYRG
                     DTRYDPEDLDGKGANPRIEEILKARQTIICQVTKNPIGAKGARLTQEISLPGRFVVLV
                     PNSTTYGISKRLPDNERKRLRQILDKAKPKGHGLIVRTAAEGISAEELARDVERLAKQ
                     WEQIEALAAKSQAPTLLYRDPDMSIRVIREEFNQDYRQVVIDDDQVFEQVRDYVRSIS
                     PALVERVERYDPAAHGGLPLYESLHVHEQLHKALDRKVWLPSGGSLIIERTEALTVID
                     VNTGKNVGKSSLEETVYRNNLEAAEEVARQLRLRDIGGIIVIDFIDMEIKKNREDVIA
                     TFRQALARDKTRTQVFDISELGLVEMTRKRIGEGLLESLSSPCPHCDGRGMVFDEELT
                     GHQVIRR"
     gene            2729..3037
                     /gene="rplU"
                     /locus_tag="VNQ33_10300"
     CDS             2729..3037
                     /gene="rplU"
                     /locus_tag="VNQ33_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWJ98540.1"
                     /translation="MYAVIKTGGKQYRVTEGQQLQVEKLAVAEGEVNLTPVLLVDGDT
                     VVSAPDALAKASVKANVIGEAKGPKIVGFTYKNKTNNRKRWGHRQQYATIEITGITKG
                     "
     gene            3042..3293
                     /gene="rpmA"
                     /locus_tag="VNQ33_10305"
     CDS             3042..3293
                     /gene="rpmA"
                     /locus_tag="VNQ33_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWJ98541.1"
                     /translation="MSKTKGGGSTRNGRDSNAQRLGVKAYDGTVVTAGSIIVRQRGTH
                     FHPGENVGKGGDDTLFATIDGQVKFGRRGGRKLVDVLPA"
BASE COUNT          601 a         1319 c         1229 g          416 t
ORIGIN      
        1 gagtcggtgg ccgagttcat cgacatcgac ctcgacgagt cctcgccccg cctgccgagc
       61 gcggccgacc tggaggccct gcccctcctg atctccgccg ccctgccgat cggtcttgac
      121 gtgaccggct gcgccaccat cgcgggcaac tcgacgtcgc tgcagcaggc ggtggagagc
      181 tgcgagtggg atgtcgagat ccgcggcgtc gagccggccg cggcggccgg tgccgtcgac
      241 cgcgtgctcg ctgcggacga cctcacgatc acccgccagc gcaagggcaa ggacgtcacc
      301 gacgatgtcc gcccctacgt gctggccctc gccgtcgtcg ggtccaccga cggcggcacc
      361 cgggtccacg cccacctggc cacccaaccc cgcgggttgc gggtgagcga gctgctcgcc
      421 ctcctcgacc cgacctggga cgagggcctg gtgcgccgga ccgcccaatg gacgctgatc
      481 gacggcgccc gacacgagcc cctcgaaggg ccggtcgagg cgacgtcgac gtcgcacgcc
      541 acggcgcgtg cgtcatgaga agggaccgca cccatgaccg acacggacgc cggggatctc
      601 cccacgccca cacccgcctc gaccgacgcg tccggcgcgc cgaaggcgga tccgaagctg
      661 atcgcgaggg cggacccggc gcccgccgct gagcccaagc cggccggcgg cagcgccgac
      721 gctggtggat cagacggcgc cagcggcggc gagaccgccg agggcggacc cggcgtggag
      781 gccaagcccc ggcgccgcgg atcgcgcggc ggccgcaacc gcaaccgccc ccgacccgag
      841 ggctcgacgg acgacccggc ggacggcgct ccccccgagg ccggcgatcg accgccgcgc
      901 tccggatcgg cgaagcaagg cccgaagggt gccggtgatc gccaacccga gctgcccgac
      961 cgccaccggg agaacaagcc ctcggcggag gccgcagagg ccgcgctggt ccgcaagccc
     1021 cagatcggcg actcgcgccc ggctccggcc gacggtgcgg gcgatgccgc ggcgaccggg
     1081 ggcggggccg agggcggcac cggcaccagc tcgtcggcca agcgccggcg tcgtcgcggc
     1141 ggccgcggtc gcggcggtgc gggcggaggc ggcggccaga gcggcaaaca gggcggcggc
     1201 aacccgggcg gtggccagcg ctccggcaac cagcgcaagg gcggcggtcg ctccggcggc
     1261 gagccggtca cggccatcac cgacgacgca ccggtcgacc tcgacgagaa gacgctcaac
     1321 aagcggcggg gcagggagcg caagggccgt ccggtcgggc gctacctgat ggccgtccac
     1381 gtgaagcccg aggccaccca gatcgccgtg ctcgagggca aggcgctcat cgagcactac
     1441 gtctcgcgcc cggccgacga caccctccag atccacggca acatctacct ggcccgggtc
     1501 cagaacgtgc tgcccggcat ggaggctgcg ttcgtcgaca tcggcacgcc gaagaacgcc
     1561 gtgctctacc ggggcgacac ccgctacgac cccgaggacc tcgacggcaa gggcgccaac
     1621 ccgcgcatcg aggagatcct gaaggcccgc cagacgatca tctgccaggt caccaagaac
     1681 cccatcgggg ccaagggcgc ccgcctcacc caggagatct ccctgccggg ccggttcgtc
     1741 gtcctcgtac ccaacagcac cacctacggc atctccaagc gcctgcccga caacgagcgc
     1801 aagcgcctcc gccagatcct cgacaaggcc aagcccaagg gccacgggct catcgtgcgc
     1861 acggcggcgg aggggatctc cgccgaggag ctcgctcgcg acgtcgagcg cctcgccaag
     1921 cagtgggagc agatcgaggc gctagccgcc aagtcccagg cgcccacgct gctctaccgc
     1981 gaccccgaca tgtcgatccg ggtgatccgc gaggagttca accaggacta ccgccaggtc
     2041 gtcatcgacg acgaccaggt cttcgagcag gtgcgcgact acgtccgcag catctcgccc
     2101 gccctcgtcg agcgggtcga gcgctacgac ccggccgccc acggcggtct gccgctctac
     2161 gagtcgctcc acgtccacga gcagctccac aaggcgctcg accgcaaggt ctggctgccg
     2221 tcgggcggct cgctcatcat cgagcgcacc gaggccctga ccgtcatcga cgtcaacacc
     2281 ggcaagaacg tgggcaagtc ctccctggag gagaccgtgt accgcaacaa cctggaggcg
     2341 gccgaggagg tcgcccgcca gctgcggctc cgcgacatcg gcgggatcat cgtgatcgac
     2401 ttcatcgaca tggagatcaa gaagaaccgc gaggacgtca tcgccacgtt ccgccaggcc
     2461 ctggcccggg acaagacccg cacccaggtg ttcgacatct ccgagctggg cctcgtcgag
     2521 atgacccgca agcggatcgg ggaggggctc ctcgagtcgc tgtcgtcccc ctgcccgcac
     2581 tgcgacgggc gcggcatggt gttcgacgag gagctgaccg gtcaccaggt catccgtcgc
     2641 tagccgggtt ggcccggctg gtcccggccc cctacgattg tccctcggcc ctgtgtgggc
     2701 ccaactccga acgctcgaag gcagaacgat gtacgcagtc atcaagaccg gtggaaagca
     2761 gtaccgggtc accgagggac agcagctcca ggtggagaag ctggccgtgg ccgagggaga
     2821 ggtgaacctc acccccgtcc tgctcgtcga tggcgacacc gtggtgtcgg cgcccgacgc
     2881 gctcgccaag gcgtccgtga aggccaacgt gatcggcgag gccaagggtc cgaagatcgt
     2941 cggcttcacg tacaagaaca agaccaacaa ccggaagcgc tggggccacc ggcagcagta
     3001 cgcgaccatc gagatcaccg gcatcacgaa gggctgacga catgtcgaag accaagggcg
     3061 gcggttccac ccggaacggc cgcgactcca acgcacagcg cctgggcgtg aaggcctacg
     3121 acggcaccgt cgtcaccgcg ggttccatca tcgtccgcca gcggggcacc cacttccacc
     3181 cgggtgagaa cgtcggcaag ggcggcgacg acaccctgtt cgccaccatc gacggccagg
     3241 tgaagttcgg ccgtcggggc gggcgcaagc tcgtcgacgt cttgccggcg tagtcctcgt
     3301 cgctcgttcg gctgctggtc cgcccctccg agccgtcaag gcccggagat cgctgcggtc
     3361 ggcgccggac cagggttccg atcagttcgc tcggaaccga cacctcgcac tcgtggcccc
     3421 agctccgctg gggccacgag tcgcgtgtga gggggcgaac gggcgacggg tgggccgagg
     3481 gctccaccct caggccgtgc gggggagcgt gcgctcgtcc cacccgaggt ggtcgagcgc
     3541 ggcgcggtgg gcgaaccgat ccgtc
//
LOCUS       DATMFS010000547         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_42682, whole genome shotgun sequence.
ACCESSION   DATMFS010000547 DATMFS010000000
VERSION     DATMFS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42682"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="VNQ33_10310"
     CDS             <1..1832
                     /locus_tag="VNQ33_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98542.1"
                     /translation="TSDMRRAADAAALAGVVWLPDVAAARTAALEAAARNGFVPSSTV
                     TISVTKSDQSDRRLQVTIRDKAVGSFFYSSLGGKSQDFTRTSFAEYILAVPMGSPRNF
                     FGTGTLLQNYTATGITPEFLYQSVNPYCTDKVNGDRYQSGYSNGLTSGNCAGTTNDEY
                     RTSGYSMYIEAPAGRSANIDVKLYDARYAETEQTVATGVCGGNYVYPSTLTWTYGSGG
                     TNIKVTGPVKYADRNSSSSSTWNATQILDAGQSVTLAGNRLRWQYPNTVNWSKTTTAS
                     TWTLGGNDDSKNVTITGPAEYQTRSNATTSTWSATVALNGSDTYTNRGPRFRYRTADY
                     ERCTDPVIDEYRQAGTDNYTYTLYQADNTPLNDEDNPQICQKTFTPTTAFDGTSYLGS
                     KRWNTLCTITTSMPTGRYILKVTNSGTAVGSNSSNGTPSGRNDGSNQWGLVAKYATSG
                     DGLCDGRNDVKCPRVYGKDAISVRAAATTTVASFYLAEILPEHAGKKLKLELFDPGEG
                     GQNIQIMKPSGTNGNTWTPTTFNWTADTSPATSGTNVSTLDVTQNKFNGKLVQITIDL
                     TGYSPPANNDWWQIKYTFSGAVTDRTTWSARIIGDPVHLVEEY"
     gene            1885..>2578
                     /gene="obgE"
                     /locus_tag="VNQ33_10315"
     CDS             1885..>2578
                     /gene="obgE"
                     /locus_tag="VNQ33_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWJ98543.1"
                     /translation="MSGFVDQAQLNVRAGDGGAGCVSMRREAHVPKGGPDGGDGGSGG
                     SIWLVADRNVSSLLAFRDHPHRRAGSGTHGQGKKKHGHSGEDLVVAVPEGTVVHDQDG
                     VLLADLVNAGDRWLAAAGGRGGKGNARFLSNSRRVPTFAEQGEPGEERWLELELKLMA
                     DVALVGFPNVGKSTLISVISAAKPKIADYPFTTLEPNLGVVRLDGGDEFVVADIPGLI
                     EGASEGRGLGHQF"
BASE COUNT          483 a          929 c          832 g          334 t
ORIGIN      
        1 ggacgtcgga catgcgccgg gcggccgacg cggccgcgct cgcgggtgtg gtgtggctgc
       61 ccgacgtggc cgcggcccgc acggccgcgc tcgaagccgc ggcgcgcaac ggcttcgtgc
      121 cgtcgagcac ggtcacgatc tccgtcacga agagcgacca gtcggaccgg cgcctgcagg
      181 tgaccatccg agacaaggcg gtcggcagct tcttctactc gtcgctcggc ggcaagtcgc
      241 aggacttcac gcgcacgtcc ttcgccgagt acatcctcgc ggtgcccatg ggcagtcctc
      301 ggaacttctt cgggacgggc acgctgctcc agaactacac cgcgaccggg atcacgcccg
      361 agttcctcta ccagtcggtg aacccgtact gcaccgacaa ggtcaacggc gaccggtacc
      421 agagcgggta ctccaacggc ctcaccagcg gcaactgcgc gggcacgacg aacgacgagt
      481 accgaacgtc gggctattcg atgtacatcg aggcaccagc aggccgatcc gcgaacatcg
      541 acgtcaagct ctacgacgcc cgctacgccg agaccgagca gacggtggcg accggcgtct
      601 gcggagggaa ctacgtgtac ccctccaccc tcacctggac ctacgggtcc ggtgggacga
      661 acatcaaggt gacggggccg gtgaagtacg cagaccgcaa ctcctcctcg tccagcacgt
      721 ggaacgcgac ccagatcctc gatgccggcc agtcggtcac gctcgccggg aaccgcctgc
      781 ggtggcagta cccgaacacc gtcaactggt cgaagaccac gacggcgtcc acctggacgc
      841 tcggcggcaa cgacgacagc aagaacgtga ccatcacagg ccccgcggag taccagaccc
      901 ggtcgaacgc cacgacctcg acctggagcg cgacggtcgc cctcaacggc agcgacacct
      961 acaccaaccg cggaccgcgg ttccgctacc gcaccgctga ctacgagcgg tgcacggatc
     1021 cggtgatcga cgagtaccgc caggcgggca ccgacaacta cacgtacacc ctgtaccaag
     1081 cggacaacac gccgctcaac gacgaggaca acccccagat ctgccagaag acgttcacac
     1141 ccacgacggc cttcgacggc acctcgtacc tcgggtcgaa gcggtggaac accttgtgca
     1201 cgatcaccac ctccatgccg acgggccggt acatcctgaa ggtgaccaac agcgggaccg
     1261 cggtcggctc gaacagctcg aacggcacgc ccagcggccg caacgacggc tccaaccagt
     1321 ggggcctcgt cgccaagtac gccacgtcgg gcgacggact ctgcgacggc cgcaacgacg
     1381 tcaagtgccc gcgcgtgtac ggcaaggacg ccatctcggt ccgcgccgcg gcgaccacca
     1441 cggtggcgtc gttctacttg gccgagatcc tgcctgagca cgcgggcaag aagctcaagc
     1501 tcgagctctt cgatcccggc gagggcggtc agaacatcca gatcatgaag ccgagcggca
     1561 ccaacggcaa cacctggacg ccgaccacgt tcaactggac ggccgacacc tcaccggcga
     1621 cgtcgggcac caacgtctcg accctcgacg tgacccagaa caagttcaac ggcaagctgg
     1681 tccagatcac catcgacctc acgggctact ccccgccggc caacaacgac tggtggcaga
     1741 tcaagtacac cttctccggg gccgtcacgg accggaccac gtggtcggct cgcatcatcg
     1801 gcgacccggt gcacctcgtc gaggagtact gagcggtcgg tcgcatggcg gctcgcccgg
     1861 cgcgccccgg tagcctcgct gaccatgtcc gggttcgtcg atcaggcgca gctcaacgtc
     1921 cgcgccggtg acggcggggc cggctgcgtg tccatgcgcc gggaggcgca cgtccccaag
     1981 ggcggacccg atggcggtga cggcggctcc ggcggcagca tctggctggt cgcggaccgc
     2041 aacgtgtcgt cgctcctggc cttccgggac cacccgcacc gccgggcggg cagcggcacg
     2101 cacggccagg gcaagaagaa gcacggccac agcggcgagg atctcgtcgt cgcggtcccc
     2161 gagggcacgg tcgtccacga ccaagacggc gtcttgctgg ccgacctcgt caacgccggc
     2221 gaccgttggc tcgccgccgc gggtgggcgg ggcgggaagg gcaacgcccg gttcttgtcc
     2281 aacagccgac gagtgcccac cttcgccgag cagggcgagc cgggcgagga gcggtggctc
     2341 gagctggagc tcaagctcat ggccgacgtg gccctggtgg ggttccccaa cgtcggcaag
     2401 agcacgctga tctcggtgat ctccgccgcc aagccgaaga tcgccgacta ccccttcacg
     2461 accctggagc ccaacctcgg cgtcgtccgg ctcgatggag gcgacgagtt cgtggtggcc
     2521 gacatccccg gcctcatcga gggggccagc gaggggcggg gcctcggaca ccagttcc
//
LOCUS       DATMFS010000548         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_42707, whole genome shotgun sequence.
ACCESSION   DATMFS010000548 DATMFS010000000
VERSION     DATMFS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42707"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..2388)
                     /locus_tag="VNQ33_10320"
     CDS             complement(85..2388)
                     /locus_tag="VNQ33_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ98544.1"
                     /translation="MAITSLDQLDPAASLLHALSKRLGDHGLAHVERLPPRPARTATL
                     SRPLPTWLEERLDELGCPALWSHQAAAVDLLRDRQHVVVATGTASGKSLCYQVPIAEA
                     VNDPIKPGTAIALFPTKALAQDQLKGFGHLAIPNLVAATYDGDTDPDNRRWARQNANV
                     VLTNPEMLHCALLPHHERWATFLMRLRYVVIDELHVLRGVFGTHVGHLLRRLRRLCEH
                     YGSSPTFVFSSATIGEPGRLAQDVCGMPVAEITDDGSPRGERLVALVDPLGIDGPRAA
                     RSANQVTAGLVADLVRGGHRTLAFCRSRAGTEVVAAEVVRRHPDLVGRVRPYRGGYLA
                     AERREIESQLFSGQLGAVVATSALELGIDVGGLDACVLNGFPGTIASMWQQMGRAGRE
                     GQPSLSVLVAGEDQLDRYFLAHPDEVFSRAPEPAVVNLANPFVLDPHLACAAYETPLS
                     HRDERWWGSDHLDDGVHRLVLDDKLRLRARADRIRAFWAARGHPSSNMGLRSGSMSEV
                     RIAFADGTMVGTVDRSRACELVHPGAIYLHQGRPHKVVDLDLHDGTAVVEPDDGREYT
                     LARTDVALRILSVDAERPLGDLELGLGAIEVTSRVTGYQRRDVLNGQILGHEELDLPP
                     QQLTTRGYWWVIPPDVLRQARVDDATVPGALHAAEHAAIGILPLFTICDRWDVGGVST
                     PWLDDTDGPTIVIYDGHPGGAGIAEMGFEFADRQLQATLDVLDRCRCRDGCPSCVQSP
                     KCGNWNEPLDKHAAKRLIRAAGLPDPA"
     gene            2607..2969
                     /locus_tag="VNQ33_10325"
     CDS             2607..2969
                     /locus_tag="VNQ33_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ98545.1"
                     /translation="MDLRLEVSERGGWSVVEVGGEIDVATAPRLREQLIALVNDQRYH
                     IVVSLEAVDFIDSTGLGVLISGLKRVRTHGGRFVLVCTEPRILKVFEITGLLAVFNVC
                     SSIEEAIVLEAEAAEEAP"
BASE COUNT          438 a         1061 c         1218 g          443 t
ORIGIN      
        1 gtgggcccgc tcgtgggcga ccccaccctc accgccgagg tcgtgatgcg cgccgaggac
       61 cccagcggct gatcgacccg aggctcaggc ggggtcgggg aggccggcgg ctcggatgag
      121 gcgcttggcg gcgtgcttgt ccagcggctc gttccaattg ccgcacttgg gggactggac
      181 gcagctgggg cagccgtcgc ggcaacggca gcggtcgagc acatcgaggg tggcctggag
      241 ctggcggtcg gcgaactcga agcccatctc ggcgatgccg gcgccaccag ggtggccgtc
      301 gtagatgacg atggtgggcc cgtccgtgtc gtcgagccac ggggtggata cgccgcccac
      361 gtcccagcgg tcgcagatcg tgaacagcgg gaggatgccg atggcggcgt gctcggcggc
      421 gtggagcgcg ccggggacgg tggcgtcatc gacgcgggcc tggcgcaaca cgtcgggcgg
      481 gatgacccac cagtacccgc gggtggtcag ctgctggggc ggcaggtcca gctcctcgtg
      541 accgaggatc tggccgttca gcacgtcgcg ccgctggtag ccggtgaccc ggctcgtgac
      601 ctcgatggcg ccgaggccca gctccaggtc gccgaggggc cgctcggcgt cgaccgacag
      661 gatccgcagt gccacgtcgg tgcgggcgag cgtgtactcc cggccgtcgt cgggctcgac
      721 gacggcggtg ccgtcgtgca ggtcgaggtc gaccaccttg tgaggacggc cctggtgcag
      781 gtagatcgcg ccggggtgca ccagctcgca cgcccggctg cgatcgacgg tgccgaccat
      841 ggtcccgtcg gcgaaggcga tgcggacctc gctcatcgaa ccgctgcgca ggcccatgtt
      901 cgaagacggg tggccccgag ccgcccagaa ggcgcggatg cggtcggccc gggcccggag
      961 gcggagcttg tcgtcgagca cgaggcggtg gacgccgtcg tcgaggtgat cgctgcccca
     1021 ccaccgctcg tctcggtggc tgagcggcgt ctcgtaggcg gcgcacgcca ggtgcgggtc
     1081 gagcacgaac gggttggcca ggttgaccac cgccggttcc ggtgcgcggg agaacacctc
     1141 gtcggggtgg gcgaggaagt agcggtcgag ctggtcctcg ccggccacca ggaccgacag
     1201 cgacggctgg ccctcgcgcc cggcgcggcc catctgctgc cacatggagg cgatggtgcc
     1261 ggggaagccg ttgagcacgc aggcgtcgag cccgcccacg tcgatgccca gctcgagcgc
     1321 cgaggtggcg accacggcac ccagctggcc gctgaagagc tgcgactcga tctcccggcg
     1381 ctcggccgcc aggtacccgc cgcggtacgg gcggacccgg ccgacgaggt cggggtggcg
     1441 gcgcacgacc tcggccgcca ccacctcggt gcccgcccgg ctgcggcaga aggcgagggt
     1501 gcggtgcccg cctcggacga ggtcggccac cagccccgcg gtcacctggt tggcagagcg
     1561 ggccgcccgg gggccgtcga tgccgagcgg gtccacgagg gcgaccagcc gctccccgcg
     1621 gggcgagccg tcgtcggtga tctcggccac cggcatgccg cacacgtcct gggccaggcg
     1681 gccgggctcg ccgatggtgg ccgacgagaa cacgaacgtc ggcgacgagc cgtagtgctc
     1741 gcacagccgg cgcagccgcc ggagcaggtg gccgacgtgg gtcccgaaca cgccgcgcag
     1801 cacgtgcagc tcgtcgatga cgacgtagcg caggcgcatc aggaacgtcg cccacctctc
     1861 gtggtgcggc agcagggcgc agtgcagcat ctccgggttg gtgaggacga cgttggcgtt
     1921 ctggcgcgcc caccggcggt tgtcgggatc ggtgtcgccg tcgtaggtgg cggcgacgag
     1981 gttcgggatc gccaggtggc cgaagccctt cagctggtcc tgagccaggg ccttggtggg
     2041 gaacagggcg atggcggtgc cgggcttgat cgggtcgttc accgcctcgg cgatgggcac
     2101 ctggtagcag agcgacttcc ccgaagcggt gccggtggcg acgacgacgt gctgccggtc
     2161 gcgcagcagg tcgacggccg ccgcctggtg cgaccacagc gcggggcagc ccagctcgtc
     2221 gagccgctcc tcgagccacg tcggcagcgg ccggctcagc gtcgcggtgc gagccggccg
     2281 agggggcagc cgctcgacgt gcgccagtcc gtggtcgccc aggcgcttgc tcagcgcgtg
     2341 cagcagcgat gcggccggat cgagctgatc gagcgaggtg atggccatgg cacgtcggat
     2401 cggagaggga acaggcgttc ggaagactac acccgtgtgg ttcggatcgg gaggggccgc
     2461 ggcccgcgtc gagacacccg gtggttgacc agcgaacgaa tcgggtacga ctcccgtcgc
     2521 gcgttcgatg ggaggatcga cgcgtcctct cggccgctgg tgtccgcagt aggtttgggg
     2581 tcgaacgagc agcgcaggag cggtggatgg atctacgact cgaggtcagc gaacggggcg
     2641 gttggtctgt ggtcgaggtc ggtggcgaga tcgatgtcgc caccgcaccc cggctccgtg
     2701 aacagctgat cgccctggtc aacgaccagc gctaccacat cgtggtcagc ctcgaggcgg
     2761 tcgacttcat cgactccacc gggctcggcg tgctgatcag cgggctcaag cgggtgcgca
     2821 cgcacggcgg ccgtttcgtc ctggtgtgca cggagccccg gatcctcaag gtgttcgaga
     2881 tcaccggcct gctcgccgtc ttcaacgtct gctcgtcgat cgaggaggcc atcgtcctcg
     2941 aggcggaggc tgccgaggaa gcgccgtgag caggcccggc gcgtcggacc gggcaccacg
     3001 gtgaccgccc cgacggccgc ggccggcgac gtggtggagc tggagctccc tgcccgccca
     3061 gaggtcgtgt ccgtcgcccg tctcatcatc ggagcactcg tcgctgccga cccgctcttc
     3121 gacgaggagc gctccgccga tctgcgcctc gccatctccg
//
LOCUS       DATMFS010000549         4704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_18981, whole genome shotgun sequence.
ACCESSION   DATMFS010000549 DATMFS010000000
VERSION     DATMFS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18981"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1632
                     /locus_tag="VNQ33_10330"
     CDS             259..1632
                     /locus_tag="VNQ33_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98546.1"
                     /translation="MRTTSKAARLGAVAAGLALILAACGSSDSGSDGASKDTGNEKTT
                     TTAADETTVTATPADKNGGRTEGDGTLQIGAILPQSGNLDFLGPPMIEGAKMAIADIN
                     KAGGVLGQDVAFTVKDDGGGGDDDLAATSTDELINNEKVDAILGAAASGTTKSVIDRI
                     VSSGTTQCSPSNTGSDLTTWDDDGLYFRTAPPDNLQAQALAKAVSDDGHTNVAIIAQN
                     TDYGTGFVEFLDPALTSNGAKVVENVTYEADGTSFDSEVETIVGAKPDAVVLVGYPED
                     GGKVIAELIKQGAGPSDLPLYVTDGLQSNELYKLVDESDPASTEGIRGTAASAAPEDG
                     ASFFPDAFKAFAPKVESPIYSAQSYDCVVLFALAAEKSQSDAPFDIAKEMVNVSNGES
                     DDAEKCSTYADCLALLKEDKDIDYDGASGALDFSEFGEPSAGSYDVYTFIADGTYKVD
                     EQVVVGS"
     gene            complement(1740..2177)
                     /locus_tag="VNQ33_10335"
     CDS             complement(1740..2177)
                     /locus_tag="VNQ33_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ98547.1"
                     /translation="MRCIDVNVLVYAHRSDVPEHEAYRTWLDLARRGPEPLAIPSAVA
                     SGFLRIVTHPRVFQVPTPVDAALDFLTVVHRSPTVERLTPGERHWPIFVDLCRRIGAR
                     GNQVPDAYLAAMAIEQGATWVSADRGFAGYPGLRWTHPVDPTT"
     gene            complement(2164..2421)
                     /locus_tag="VNQ33_10340"
     CDS             complement(2164..2421)
                     /locus_tag="VNQ33_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWJ98548.1"
                     /translation="MRTTITIDEQVYREAKAMAATSSRTVSELIEDAVRTALRAGERP
                     SGELEPLPTFGHGGVHAGIDLAHRSSLTDAMDAGDALDALH"
     gene            complement(2484..3353)
                     /locus_tag="VNQ33_10345"
     CDS             complement(2484..3353)
                     /locus_tag="VNQ33_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98549.1"
                     /translation="MTDPTEPTDPTGPVDPIGGTGGPAVEDPTAAAPLPPEAATHLEH
                     PDTDRPVLMTATDLVAGYLPGVDILNGASLELRTGELVGIIGPNGAGKSTLIKTLFGL
                     VPVRSGTVVLDGEDITSKPAHELVAQGVGYVPQTKNVFPSLTIEENLRMGIFLDPSKF
                     DVRFERVVSLFPLLADRRKERAGLLSGGQRQMVAMGRALMTDPSVLLLDEPSAGLSPI
                     NQDEVFARIDEVKRAGVSVVMVEQNARKCLQIADVAFVLDQGRNAYTGTGRELLDDPT
                     VIELYLGTLARDL"
     gene            complement(3350..4207)
                     /locus_tag="VNQ33_10350"
     CDS             complement(3350..4207)
                     /locus_tag="VNQ33_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98550.1"
                     /translation="MDADAATTADPFAAVAPTPGVAKPDPILVADGVQRRYGGLTAVD
                     VDHLELQRGVITSLIGPNGAGKTTFFNLLTGFDKANEGTWTFEGVKANGVRPHHLARR
                     GMVRTFQLTKALGRMTVLDNMLLAGPDQTGESALKSLVKPLWRDQETANVDKAHELLT
                     RFKLDALADEYAAGLSGGQRKLLEMARALMTDPTLVMLDEPMAGVNPALTQSLLGHVT
                     ALRDEGMSVLFVEHDMDVIMTISDWVVCMAEGSIIAEGRPADIAGNDAVIEAYLGIDH
                     GGATTGEIR"
     gene            complement(4197..>4704)
                     /locus_tag="VNQ33_10355"
     CDS             complement(4197..>4704)
                     /locus_tag="VNQ33_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ98551.1"
                     /translation="WSMLVCWTLVLLSTLLVRTWVRSPWGRVLRAIREDEDAARALGK
                     NVVVYKLQSFVIGGVIGSLAGAMMALDSGSVGPARWIAPLTFALYTVVILGGAGTVWG
                     PIVGAVVYWFVLDFADGALNNLWFDVSDTTSGAFRLTLMGIGLMLLMVFRPQGILGNK
                     KEAFASGR"
BASE COUNT          755 a         1541 c         1712 g          696 t
ORIGIN      
        1 gtctcgcggt cgccgatgag gtacacgaag ttgaccatct gctgggcgac gggatcggac
       61 tcggcgatgt ccatcccggc gaggatctgg cggaagtaga cgcgggaggt gtcggccatg
      121 gccgcatcgt acggcggcgc cgctgccgga ggccccggtg cgtgcgaact cggccccggt
      181 ggcggatcgc gagcgccgcc gtcggcagcg ccctaggttt ccccaggcga cctactcgca
      241 aaacgaaggg gaaacggaat gcgaacgacc agtaaggcag cgcggctcgg tgccgttgcg
      301 gcgggtcttg cgctgatcct tgccgcctgt ggcagcagcg acagcggctc ggacggagcg
      361 agcaaggaca ccggcaacga gaagaccacg acgaccgcgg ccgacgagac cacggtcacg
      421 gcgacgccgg ccgacaagaa cggcggccgc accgagggcg acggcaccct ccagatcggg
      481 gcgatcctcc cgcagtcggg caacctcgac ttcctcggcc cgcccatgat cgagggcgcc
      541 aagatggcca tcgccgacat caacaaggct ggcggcgtcc tcggccagga cgtggcgttc
      601 accgtcaagg acgacggcgg cggcggtgac gacgaccttg ccgccacctc caccgacgag
      661 ctgatcaaca acgagaaggt cgacgccatc ctcggtgccg ccgcctcggg caccacgaag
      721 tcggtcatcg accgcatcgt gtcgtcgggc accacgcagt gctcgccgtc gaacacgggc
      781 tcggacctca ccacctggga tgacgacggc ctgtacttcc gtacggcacc gccggacaac
      841 ctccaggcgc aggccctcgc caaggccgtg tccgatgacg gccacaccaa cgtggcgatc
      901 atcgcccaga acaccgacta cggcaccggc ttcgtcgagt tcctcgaccc ggccctcacc
      961 agcaacggtg cgaaggtcgt cgagaacgtc acctacgagg cggacggcac gtcgttcgac
     1021 tccgaggtcg agaccatcgt cggtgccaag cccgacgccg tggtcctcgt cggctacccg
     1081 gaggacggcg gcaaggtcat cgccgagctg atcaagcagg gcgccggccc gtccgacctc
     1141 ccgctgtacg tcaccgacgg cctccagagc aacgagctgt acaagctggt ggacgagtcc
     1201 gatccggcct cgaccgaagg catccgcggc accgctgctt cggcggctcc cgaggacggc
     1261 gcgtcgttct tccccgacgc cttcaaggcc ttcgctccca aggtcgagtc gccgatctac
     1321 tcggctcagt cctacgactg cgtggtcctc ttcgccctgg cggcggagaa gtcgcagtcc
     1381 gatgccccct tcgacatcgc caaggagatg gtcaacgtct ccaacggcga gtccgacgat
     1441 gccgagaagt gcagcaccta cgccgactgc ctcgcccttc tcaaggagga caaggacatc
     1501 gactacgacg gtgcctcggg tgccttggac ttctcggagt tcggtgagcc cagcgccggt
     1561 tcgtacgacg tgtacacgtt catcgccgac ggcacgtaca aggtcgacga gcaggtcgtc
     1621 gtcggctcct gagcctgagg atctgatcgc agatcactga tggggcgggg ccgcgaggcc
     1681 ccgccccatc gcgttcgccc agcgttcgct ggtggatgcc gcgccgtggg cgggtcgtct
     1741 caggtggtcg ggtcgaccgg gtgcgtccag cgcaggccgg gatagccggc gaagccccga
     1801 tcggcgctga cccacgtcgc gccctgctcg atggccatgg ccgcgaggta ggcgtcgggc
     1861 acttggttgc ctcgggcccc gatgcggcgg cagaggtcga cgaagatggg ccagtgccgc
     1921 tcgccgggcg tcagtcgctc gaccgtcggc gatcgatgga ccacggtgag gaagtcgagg
     1981 gcggcgtcga ccggggtggg cacctggaag acgcgcgggt gggtgacgat ccggaggaac
     2041 ccgctggcga cggcggatgg gatcgcgagc ggttcggggc cacgacgagc cagatcgagc
     2101 caggttcggt aggcctcatg ctctggcaca tcggaccggt gcgcgtacac caagacgttg
     2161 acgtcaatgc agcgcatcga gcgcgtcccc ggcgtccatc gcgtcggtca gcgacgagcg
     2221 gtgggcgagg tcgatgcccg catggacgcc gccgtgaccg aaggtgggca gcggctcgag
     2281 ctcgcccgat ggacgttcgc ccgctcggag cgcggttcgg accgcatctt cgatcagctc
     2341 gctcaccgtc cgggaggacg tggccgccat cgccttcgct tcgcggtaca cctgctcgtc
     2401 gatcgtgatc gtggtccgca tgggtgcatc ttagcatcaa gggttgatgc aaagatgcac
     2461 gacggtacga cggccgagca cgctcagagg tcgcgggcga gggtgccgag gtagagctcg
     2521 atcaccgtgg ggtcgtcgag aagctctcgg ccggtgccgg tgtaggcgtt gcgaccttgg
     2581 tcgaggacga aggcgacgtc cgcgatctgg aggcacttgc gggcgttctg ctcgaccatc
     2641 acgacggaga cgccggcgcg cttcacctcg tcgatgcggg cgaagacctc gtcttggttg
     2701 atgggcgaca ggccggccga tggctcgtcg agcaggagca ccgacggatc ggtcatcagc
     2761 gcccggccca tggccaccat ctggcgctgg ccgcccgaga gcaggccggc ccgctccttg
     2821 cggcggtcgg ccaggagcgg gaagagggag acgacgcgct cgaagcggac gtcgaacttg
     2881 gacggatcga ggaagatccc catccgcagg ttctcctcga tggtgagcga ggggaacacg
     2941 ttcttggtct ggggcacgta gcccacgccc tgggcgacca gttcgtgggc cggcttggac
     3001 gtgatgtcct cgccgtcgag cacgacggtg ccgctgcgca ccgggaccag gccgaagagg
     3061 gtcttgatga gcgtcgactt gccggcgccg ttgggcccga tgatgccgac cagctcgccc
     3121 gtgcggagct cgagcgaggc gccgttcaag atgtcgacgc ccggcaggta gccggccacg
     3181 aggtcggtgg cggtcatgag cacgggccgg tcggtgtcgg ggtgctcgag gtgggtcgcc
     3241 gcctccgggg gcaacggtgc cgctgccgtc gggtcctcga cggccggacc gccggtgccg
     3301 ccgatcgggt cgaccggacc cgtcgggtcg gtgggttcgg tcgggtcggt catcggatct
     3361 ccccggtggt ggcgccaccg tggtcgatgc cgaggtaggc ctcgatgacc gcgtcgttgc
     3421 cggcgatgtc ggcgggacgg ccctcggcga tgatcgaccc ctcggccatg cagacgaccc
     3481 agtcgctgat ggtcatgatc acgtccatgt cgtgctccac gaacaggacc gacatgccct
     3541 cgtcgcgcag cgcggtgacg tggccgagga ggctctgggt gagcgccggg ttcacgccgg
     3601 ccatgggctc gtcgagcatg acgagcgtcg ggtcggtcat gagggcccgg gccatctcca
     3661 gcagcttgcg ctggccaccc gagagaccag cggcgtactc gtcggccaga gcgtcgagct
     3721 tgaagcgggt gagcagctcg tgggccttgt cgacgttggc cgtctcctgg tcccgccaca
     3781 gcggcttgac cagggacttg agcgccgact cgccggtctg gtcgggtccg gccagcagca
     3841 tgttgtcgag gaccgtcatg cggccgagcg ccttggtgag ctggaaggtg cggaccatgc
     3901 cccggcgggc gaggtggtgg gggcggacgc cgttggcctt cacgccctcg aaggtccagg
     3961 tgccctcgtt ggccttgtcg aagccggtga gcaggttgaa gaacgtcgtc ttgccggccc
     4021 cgttggggcc gatgagcgac gtgatgacgc cgcgctggag ctcgaggtgg tcgacgtcga
     4081 cggcggtgag cccgccgtag cggcgctgca cgccgtcggc cacgaggatc gggtccggct
     4141 tggcgacgcc cggggtgggg gcgacggcgg cgaaggggtc ggccgtggtg gcggcgtcag
     4201 cgtccactgg cgaacgcctc cttcttgttg ccgaggatgc cctgcggccg gaacaccatg
     4261 agcagcatca ggccgatgcc catgagggtc aggcggaacg cgccggacgt ggtgtcgctc
     4321 acgtcgaacc agaggttgtt gagggcgccg tcggcgaagt cgagcacgaa ccagtagacg
     4381 acggcgccga cgatgggacc ccagaccgtg ccggcgccac cgaggatgac cacggtgtag
     4441 agggcgaagg tgagcggggc gatccagcgg gccggaccca ccgagccgga gtcgagcgcc
     4501 atcatcgccc cggcgaggga cccgatgacg ccgccgatca cgaacgactg gagcttgtag
     4561 acgacgacgt tcttgccgag ggcccgggcg gcgtcctcgt cctctcggat ggcccggagc
     4621 acccggcccc acgggctgcg gacccaggtg cggacgagca gcgtggagag gagcacgagg
     4681 gtccagcaca cgagcatcga ccag
//
LOCUS       DATMFS010000550         9193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_272433, whole genome shotgun sequence.
ACCESSION   DATMFS010000550 DATMFS010000000
VERSION     DATMFS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272433"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..284
                     /locus_tag="VNQ33_10360"
     CDS             81..284
                     /locus_tag="VNQ33_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98552.1"
                     /translation="MRTSEHATRATSAVVIYVVILVAFQVFLLTVAVEAFASDDEPLA
                     WATAAVSVSLATGSAVLLRYLRP"
     gene            281..1342
                     /locus_tag="VNQ33_10365"
     CDS             281..1342
                     /locus_tag="VNQ33_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HWJ98553.1"
                     /translation="MTAPEGRLIEANIRTLFPGYFAMVMATGIIAIGADQQDLRWLGQ
                     PLYVITAIAYVVLSVLLAWRGVVASKLLAVDITSHAKGFAFLTTVAATNVLGSASAVI
                     HGWWTAAWVLWWFSLALWVFFVYATLFAVVLKGPKPGLDKGINGTWFLLTVSAESIVV
                     LGALLLGRHTSDLFAFALIAAFALGLVLYLIVMTMVFLRWTFAELEPTEADPPAWIAA
                     GAVAITVLAGSNLLGARSSSLRIERLAPFIEGVVVLAWATATFWFPLMVAIGIWRHVV
                     NRVPLRYHPSYWALVFPLGMYGAATFRMRAAIGLDALAWLPEVTLAVALAAWTAAFLG
                     LLLEAARAVSRLNGSRHRR"
     gene            complement(1829..2068)
                     /locus_tag="VNQ33_10370"
     CDS             complement(1829..2068)
                     /locus_tag="VNQ33_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98554.1"
                     /translation="MSDSYDLWYDATTVDGHLGVTCWISHGPRADDPRFDPQPGDVVV
                     VGDDEEEPLSARVVNRDGDRVTVQLAIAGVSAVAS"
     gene            complement(2086..2472)
                     /locus_tag="VNQ33_10375"
     CDS             complement(2086..2472)
                     /locus_tag="VNQ33_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98555.1"
                     /translation="MEPVNLRDREVPAVLAVVRLGTVTLEDAALTKSVAECHERWGMW
                     GFSVLEVPDGDYAVLARLRPIVATRRRLLVASGPALVAAGFPLLPTLDHPHWTVVLSE
                     PSPGQFARVRALFEGPIDNPAWTRPT"
     gene            complement(2510..3223)
                     /locus_tag="VNQ33_10380"
     CDS             complement(2510..3223)
                     /locus_tag="VNQ33_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98556.1"
                     /translation="MILAWPLTISTELAGVLRPGGHLRADSALRSLRDKGLAAEPITV
                     VPRSGRGADGRVVWYSSLMVDVARLVRAGDLGTAEAAHAAATELAGHRAARFVAEWLA
                     EHGGPDPDPAEIDLGTGGALTRLATLTASARARLLSDLRIDTFAGRVADVSGRVAFVV
                     DEEGRSLPLPAPSGAPMTWAGALVVVDTEELSGGATAVWVRPAFDPEADLNERVPGGP
                     HLLTSAERERLSRRVAASR"
     gene            complement(3375..4871)
                     /locus_tag="VNQ33_10385"
     CDS             complement(3375..4871)
                     /locus_tag="VNQ33_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98557.1"
                     /translation="MFEAHIDLAGRMSITRRLPDDEEVFESLASRLRPLTVERESVFY
                     ETVLGAIECLVDPGMYGDEHREWLSQLRQAWRAAEIQGTEAQGYVVQSAQIDGTAPTG
                     LISDTQLAAAWLYADLVHVDAKGPKEDALAFSLRERYAAAVRLFSHLAMLTVSTLRLV
                     ESLREAGALFIAESAWDDEVVVRASELVEEARVFVAPVDASIPDMRESMAFGEDWRPF
                     TVTELLRQDPANQVQVTLTDDDGATVVSYDAALSRNHADDAITWQVLVAGSVLFKFAF
                     DLHDGQVSAARFIGCDFLDTTNALKLASTQARIQLHHAASAVFTVQDQELFTLSSPSL
                     SPEMLRDLEVVAEAVGDVVAIEHLTGQDLDPCNEEFDDRQRVYLRRARLMWEGHIVQA
                     MRHPVSVTVPTGRPPQVLLAAAGTLDVGGAQVPTPRMLLRHPNMNAVEQRDVDQGSPT
                     TRRYMVQPPEGGAFMAWSPDRVQVSNDDELVVSAPWDLLDIAEATFGY"
     gene            5121..5573
                     /locus_tag="VNQ33_10390"
     CDS             5121..5573
                     /locus_tag="VNQ33_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98558.1"
                     /translation="MNRRTPRHRGRPNAEDKPVPGTTRTPELTAMHNFTDIADRAVVT
                     FFHHDGFNGPMGGIAHGFDPVTRFEAVTGFDVSFTRRLGWEELADAHIVAKHELIEYF
                     NKAMNHPYAGEDDVESLTLLVHRQLAGWYPHRSTRRRVPLQLGPTPIR"
     gene            complement(5825..5897)
                     /locus_tag="VNQ33_10395"
     tRNA            complement(5825..5897)
                     /locus_tag="VNQ33_10395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5862..5864),aa:Gly,seq:gcc)
     gene            complement(5952..7298)
                     /locus_tag="VNQ33_10400"
     CDS             complement(5952..7298)
                     /locus_tag="VNQ33_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWJ98559.1"
                     /translation="MQCPACTAENDPSSKFCMACGSALPAADPTVEAPADGAVAAWAS
                     ATDEGVLAADETAPRVDRPSWLADEADDADVAGAAGPSADGDEPAGTEADLQGAPAEA
                     EPASEGEPATAVVDAVPDATPGDATPPPWTPAGEERPAPHHEAELPPPPPPSGFAPPP
                     PPPPSPAAPPPPSGYPAPPAPAAYPPPPAAPGAYPPPPPPGPAAPPSASPPPPPGQGF
                     APSAPPPPPNPGAWIPPAPGWTQNPPPPPAPVPPQGYAPPPPPSGYPPPPGPQGYGQA
                     PLPPPAPQGYGHVPPPPPPAQPAWGQPAPGAYAPPPPPGATGAPDPNGLGLAFGRLGS
                     GARKSAKAAVTVAGALLDEGETVEAVVAGRLEGHGAVVVLSDRSLRLVDDREWRPFHE
                     QLPLGPGLEVQGWQDDRTASLTFVFGGRSLVLDQIPDRELAVEMAGRIRYRAGATA"
     gene            complement(7364..7945)
                     /locus_tag="VNQ33_10405"
     CDS             complement(7364..7945)
                     /locus_tag="VNQ33_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HWJ98560.1"
                     /translation="MSARFAAAVAAIDAANAADPVRVTVDGVDRPKELVHADLVERWV
                     RTLDPGASELQVLAARAHHLRRWVVPRSDYPEGRAGYLRWRTDHKKRQAAEVAEILRA
                     AGYDEDEVEQVAGIVAKRNLAADPAVQTHEDALCLAFLQLQFDELTDQLGDGHMVEVV
                     RKTMAKMSPAALAAAGSLTVSDRGARILSAASA"
     gene            complement(7942..8163)
                     /locus_tag="VNQ33_10410"
     CDS             complement(7942..8163)
                     /locus_tag="VNQ33_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98561.1"
                     /translation="MADSLDIDAMLARFRDRAMAVRKRNLPPVGGEERTRLVEQAQTD
                     FQDFAIIGDAAGTLDDGILTLTIDLRPKG"
     gene            complement(8209..8844)
                     /locus_tag="VNQ33_10415"
     CDS             complement(8209..8844)
                     /locus_tag="VNQ33_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98562.1"
                     /translation="MAIRRRTLLFVLALGFSAAVAPAALATAAPAQDPGATFGTGETA
                     TTVAGSTSAPTATTAATDSTNQTLVESEDAPEKPIFTESRKVAAIIGGLVLVALAILL
                     LTVRYIRLTKPVAQGDISDLPFLADDVDVEDDDVFIAELPPKADPVQPVVPVAVPEVA
                     LGAAPGTDHASADADWESRTGEHQRVEIPGGTSLARPGVAARRKALGITAD"
     gene            complement(8864..>9193)
                     /locus_tag="VNQ33_10420"
     CDS             complement(8864..>9193)
                     /locus_tag="VNQ33_10420"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98563.1"
                     /translation="RAPLKARLLDQSRIAGLGNLLVDETLWRARLDPTREAGSLTGAE
                     LRRLQRRIGSTVGQLFERGGSHRGDLQVERHRDGHCPRCGTPLERGTVGGRTTYWCRP
                     CARAATA"
BASE COUNT         1431 a         3224 c         3191 g         1347 t
ORIGIN      
        1 agcggcccac cgccacggcc gctgggccgc atcgccgtgg agatccgcga cgacaccatc
       61 tgggccctcg ggtaccagcc atgaggacct cggagcacgc cacccgcgcc acgtcggcgg
      121 tcgtgatcta cgtcgtcatc ctcgtcgcgt tccaggtctt cctcctcacc gtggccgtcg
      181 aggcgttcgc cagcgacgac gagcccctcg cctgggcgac cgcggccgtc tccgtgagcc
      241 tcgccaccgg ctccgccgtc ctgctccgct acctccgtcc atgaccgccc ccgagggccg
      301 cctcatcgag gcgaacatcc ggacgctctt ccccgggtac ttcgccatgg tcatggcaac
      361 cggcatcatc gcgatcgggg cggaccagca agacctgcgc tggctcggcc agccgctcta
      421 cgtgatcaca gccatcgcct acgtggtcct gtcggtcctc ctggcctggc ggggcgtcgt
      481 cgcctcgaag ctgctcgccg tcgacatcac cagccacgcc aagggcttcg cgttcctcac
      541 caccgtggcg gcgaccaacg tgttgggcag cgcatcggcc gtcatccacg gctggtggac
      601 cgcagcgtgg gtgctctggt ggttcagcct cgccctctgg gtgttcttcg tctacgcgac
      661 cctcttcgcc gtggtgctca agggcccgaa gcccggactc gacaaaggca tcaacggcac
      721 ctggttcctg ctcaccgtct cggccgagtc catcgtcgtg ctcggcgccc tgctgctggg
      781 tcggcacacc agcgatctgt tcgccttcgc cctgatcgcc gcgttcgcgc tcggcctcgt
      841 cctctacctg atcgtcatga ccatggtctt cttgcgctgg accttcgcgg agctcgaacc
      901 caccgaagcc gacccacccg catggatcgc agcgggcgcc gtcgccatca ccgtcctcgc
      961 cgggtcgaac ctgctggggg cgcggagcag ctcgctgcgg atcgagcgcc tcgccccgtt
     1021 tatcgagggc gtcgtcgtgc tggcgtgggc caccgccacg ttctggttcc cgctcatggt
     1081 cgccatcggc atctggcgcc acgtcgtcaa ccgcgtcccg ctccgctacc atccctcgta
     1141 ctgggccctc gtgttcccgt tggggatgta cggggccgcg accttccgca tgcgggccgc
     1201 catcgggctc gacgccctcg cgtggctccc cgaggtcacc ctcgccgtcg cactcgccgc
     1261 atggaccgca gcgttcctcg gcctcctcct cgaagccgca cgggcggtgt cgcgcctgaa
     1321 cggctcccgt cacaggcgct gagcacgaag aggcgccagg ctgggatcga cggcacccgc
     1381 tctggcaccg accggccgcc aagcgtcgag caccccatcc tcttgggcat ccccaccgat
     1441 ctcgaacgac gggcaccttc accgacgctc cgaaccccac cggcggggtc actccccgcc
     1501 cgggctcaac ggccctgggt cccacgaatc tggatccagt cggtaccggc acggtcaagc
     1561 ggtcgccgtc gcggctcgca tcgaggaaac cgctggtgag aacgacgaaa ccccctgcgt
     1621 agcagggggt ttcgctccga gcggacgacc ggattcgaac cggcgaccct caccttggca
     1681 aggtgtggcc cgactggctc agcggcgacg gaacgccctg gtcagcggcg atctcccgtc
     1741 cagcggccgg tcgctggcac aacttctggc acaacggagt cgtcttgcgg cgccgtgccg
     1801 tcctgtggtg cccagtccca gcctgttcct acgaagcgac agcggagacg ccggcaatgg
     1861 ccagctgcac ggtcacgcgg tcgccatcgc ggttcacgac gcgcgccgag agcggctcct
     1921 cttcgtcgtc gccgacgacc accacgtctc cgggctgcgg gtcgaaccga gggtcatcgg
     1981 cgcgcggtcc gtgagagatc cagcacgtca cgccgaggtg gccgtcgacg gtcgtcgcgt
     2041 cgtaccagag gtcgtaggaa tcgctcacgg tcacaatgct acggctcatg tcggccgtgt
     2101 ccaggccggg ttgtcgatcg gcccctcgaa gagcgcacgc acgcgagcga actggccagg
     2161 tgacggctcg gacagaacca ccgtccagtg aggatgatcg agcgtcggca gcaacgggaa
     2221 gcccgcagcc actagggccg gcccgctggc gaccagcagt cggcgtcgag tggcgacgat
     2281 cggccgcagg cgtgcaagca ccgcgtagtc cccatccggc acctccagaa ccgagaaccc
     2341 ccacatgccc caacgctcgt ggcactccgc aacgctcttg gtcagtgccg catcctccag
     2401 cgtgacggtg ccaagacgga cgaccgcgag aacggccggg acttctcgat ctcgcaggtt
     2461 cactggctcc atcacgtact ccgtccttca gatcgaatgg gtaccgcgat catcgcgacg
     2521 cggcgacccg gcgcgacagc cgttcccgtt ccgccgatgt caggaggtgc ggcccgccgg
     2581 gcacccgctc gttcaggtcg gcctcgggat cgaacgcggg acgaacccac acggccgtgg
     2641 cgccgcccga cagttcctcg gtgtccacga ccacgagagc acccgcccag gtcatcggcg
     2701 ccccggacgg agccgggagc ggcaaggacc gcccctcctc gtcgacaacg aaggcgaccc
     2761 gaccggacac gtcggcgacc cgaccagcga acgtgtcgat gcggaggtcc gacaagagcc
     2821 gagcgcgcgc cgatgcggtc agcgtggcca accgggtgag tgccccaccg gttccgagat
     2881 cgatctcggc cgggtctgga tcaggcccac cgtgttcagc cagccactcc gctacgaacc
     2941 gggccgctcg gtgaccggcg agctcggtgg ccgctgcgtg cgcggcctcg gccgtaccca
     3001 ggtcgcccgc tcggacgagc cgagcgacat cgaccatgag tgacgagtac cagacgaccc
     3061 gaccatcggc gccccggccc gagcgcggca cgacagtgat gggctcggca gccaagccct
     3121 tgtcccgaag cgagcgcagc gccgaatcgg cgcgaaggtg ccctccggga cggagcacac
     3181 cagcgagctc cgtggagatc gtgagcggcc aagcgaggat catcgcaacg atcgtagctt
     3241 ctgaatcagt ggtttgcaga gtcagagcgc gccggctcaa ttgcgtagag gccgaacccg
     3301 tcgcgcctca tagcctggtc aacgagatgc ccaccgatcc cgcgccgcaa caccgtgagc
     3361 cctccgtacg gcgttcagta cccgaaggtg gcctcagcga tatccagtag gtcccacggc
     3421 gcggagacga cgagttcatc gtcgttcgac acctgaaccc gatcgggcga ccatgccatg
     3481 aaggcaccgc cctctggggg ctggaccatg tacctccttg tcgtcgggct gccttggtcc
     3541 acgtcacgtt gctcgacagc gttcatgttt ggatggcgaa ggagcatcct cggcgtcggc
     3601 acctgtgcgc cgcccacatc gagcgttccg gcggcagcca gcaagacctg cggtgggcgc
     3661 cccgtcggga cggtgaccga gacagggtgc cgcatcgctt gaacgatgtg gccctcccac
     3721 atgaggcggg cacgacggag gtacacgcgc tggcggtcgt cgaactcctc gttgcacggg
     3781 tcgagatctt gtccggtgag atgctcgatg gcgaccacgt ctccgacggc ttcggccacc
     3841 acttccaggt cacgaagcat ctcgggtgac aatgagggcg agctgagtgt gaagagttct
     3901 tgatcctgaa cggtgaagac tgccgacgcg gcgtggtgga gctggatccg cgcctgagtc
     3961 gaggcgagct tgagcgcgtt ggtcgtgtca aggaagtcgc agccgatgaa ccgagccgcg
     4021 gacacctggc cgtcgtggag atcgaaggcg aacttgaaga gcacgctgcc ggcgacgagc
     4081 acttgccagg tgatggcgtc gtcagcgtgg ttccgtgaga gagcggcgtc gtacgacacg
     4141 actgttgccc catcgtcgtc ggtgagcgtg acttggacct ggttcgcagg atcctggcgg
     4201 agcaactccg tgacagtgaa cggcctccag tcctctccga acgccatcga ctcgcgcatg
     4261 tccgggatcg atgcgtcaac cggcgcgacg aacacacgcg cctcttcaac gagctcagag
     4321 gccctgacca cgacctcgtc atcccatgcc gactcagcaa tgaagagcgc gccggcctct
     4381 cgtagcgact cgaccagccg cagcgtcgag accgtcagca tcgccaggtg agagaacaag
     4441 cggaccgcgg ccgcgtatcg ctcgcgcagc gagaacgcga gtgcatcttc cttcggtccc
     4501 ttcgcgtcga cgtgaacgag atcggcgtag agccacgcgg cggcgagctg ggtatcggag
     4561 atcagaccgg tcggggctgt cccgtcgatc tgcgccgact gcaccacgta gccctgggcc
     4621 tcggtgccct ggatctcagc cgcccgccac gcttgtcgaa gctgcgacag ccactctcga
     4681 tgctcatcgc cgtacatacc tggatcgacc aagcactcga tcgcaccgag caccgtctcg
     4741 tagaagaccg actctcgctc cacggtcagt ggccgcaaac gcgaagcaag cgactcgaag
     4801 acctcttcgt catccggtag tcgccgcgtg atcgacatcc gacccgcgag gtcgatgtga
     4861 gcctcgaaca cgcctcgagc gtggtggcgc agcccatccc agtcctggac aagcgagtgg
     4921 gcctggaccc tccgagcccg tagaacaaag cgcctcagcg tctcttcgtg actgacctga
     4981 gccatgagac tgatcacctc aagactgcaa ccaccagcag tcccacaaga acgtgcaggc
     5041 taccgacagc accaccggcc agcaggcgcc tttcttgctc ggatgctgag caaagtgaaa
     5101 gacgacgaac tccgctgacg atgaacaggc gtaccccgag acaccggggc cgcccgaacg
     5161 ccgaggacaa gcccgtcccc ggcaccactc gaacaccgga gctgacggcc atgcacaact
     5221 tcaccgacat cgccgaccga gctgtcgtga cattcttcca ccacgatggc ttcaacggcc
     5281 caatgggtgg catcgcccac gggttcgacc ccgtgacgag attcgaggcc gtcaccgggt
     5341 tcgacgtgag cttcacccgc aggctcggct gggaggagct ggccgacgcc cacatcgtgg
     5401 cgaagcacga gctcatcgag tacttcaaca aggccatgaa tcacccctac gcgggtgaag
     5461 acgacgtcga gagcctcacc ctcttggtcc accgccaact cgccggctgg tacccccacc
     5521 gatcgacgag gcgccgggtg ccactccagc tcggaccaac gcccatcaga tagcggaaca
     5581 ggattgttgg gctgagctcg aacgaatctg tccgctcggc tccgcacggc cgtcacgaac
     5641 ccgaatccac cgatgtgacg taaggcacaa agtgggcaca aagtgggcac aaatctgaga
     5701 gggacgaatg agaacgccct ggtaggcgat gggacagccc gagacgaccg cgaaccccgt
     5761 ttcgggcctc tgacctgggc cggaaaagca gaaaacccct gcctgagcag gggttttctt
     5821 ccggagcgga cgaccggatt cgaaccggcg accctcacct tggcaaggtg atgctctacc
     5881 aactgagcca cgtccgcgtg ggctagtcac cctagcaaga tcacccaggt cgagacgaac
     5941 ccgatcgctg gtcaggcggt ggcaccagcc cggtagcgga tgcggccggc catctcgacg
     6001 gccagctccc ggtccgggat ctggtcgagc accagggacc gccccccgaa cacgaacgtg
     6061 aggctcgcgg tgcggtcgtc ctgccagccc tgcacctcga gcccggggcc gagcgggagc
     6121 tgttcgtgga acggtcgcca ctcccgatcg tcgaccaggc ggaggctgcg gtctgagagc
     6181 acgacgacgg cgccgtggcc ctcgagtcgg ccggcgacga cggcctcgac cgtctcgccc
     6241 tcgtcgagca gcgcaccggc gacggtgacg gcggccttgg cggacttgcg ggcaccgctg
     6301 ccgagacggc cgaacgccag gccgagcccg ttgggatcgg gggcgccggt cgcaccgggt
     6361 ggaggcggcg gcgcgtacgc gcccggcgcg ggctgacccc acgcaggctg cgccggcggc
     6421 ggaggaggcg gcacgtggcc gtacccctgg ggggccggag gcggcagcgg tgcctggccg
     6481 tacccctgcg gcccgggcgg gggcgggtag cccgatggcg ggggcggcgg cgcgtacccc
     6541 tgcggtggaa cgggcgccgg aggaggcggt gggttctggg tccagcccgg tgcaggcggg
     6601 atccacgcgc ctgggttggg cggcggaggc ggggccgacg gagcgaaccc ctgcccgggc
     6661 ggcgggggtg gagaggccga aggcggagcg gccgggccgg gcggaggcgg tggcgggtag
     6721 gcccccggcg cagcgggagg cggcggatac gccgcgggag caggtggcgc cgggtacccg
     6781 ctcggtgggg gcggggcggc cgggctcggc ggaggcggag gcggtggcgc gaaccccgac
     6841 ggcggaggag gcgggggcag ctcggcctcg tggtgggggg cggggcgctc ctcccccgcc
     6901 ggtgtccacg gcggcggtgt ggcatcgccc ggcgtggcat cggggaccgc gtcgaccacg
     6961 gcggtcgccg gctcgccctc ggacgccggc tcagcctcgg caggcgcgcc ctggaggtcg
     7021 gcttcggtcc cggccggttc gtcgccgtcg gcgctcggcc cagcggcccc ggccacgtcg
     7081 gcatcgtcgg cctcgtcggc gagccagctg ggccggtcga cgcgcggcgc cgtctcgtcg
     7141 gccgcgagca cgccttcgtc ggtcgccgac gcccaggcgg cgacggcgcc atctgccggt
     7201 gcctcgacgg tgggatcggc ggcggggagg gcggacccgc aggccatgca gaacttcgag
     7261 ctcggatcgt tctcggccgt gcaagcagga cactgcatct tcgaacccct ctggtcgctg
     7321 gtggtgggga gcgtatgcga ccccgggagc cgttcgtccc cggctacgcc gaagcggccg
     7381 agaggatgcg ggcgccgcgg tcgctgacgg tgagggaccc ggcagcggcc agggcggcgg
     7441 ggctcatctt ggccatggtc ttgcggacga cctcgaccat gtggccgtcg cccagctggt
     7501 cggtcagctc gtcgaactgg agctgcagga acgccaggca gagggcgtcc tcatgggtct
     7561 gcacagccgg atcggcggcg aggttgcgct tggcgacgat gccggcgacc tgctcgacct
     7621 cgtcttcgtc gtacccggcc gcacgcagga tctcggccac ctcggcggcc tggcgcttct
     7681 tgtggtcggt gcgccagcgg aggtagccgg cccggccctc cggatagtcc gagcggggca
     7741 cgacccaccg gcgcaggtgg tgggcccggg cggccagcac ctgcagctcc gatgcgccgg
     7801 ggtcgagggt gcggacccac cgctccacga gatcggcgtg gaccagctcc ttcggccgat
     7861 cgacgccgtc gacggtgacg cgcaccgggt cggcggcgtt ggcggcgtcg atggcggcga
     7921 cggcggcggc gaaccgggcg ctcacccctt cgggcgcagg tcgatggtga gggtcaagat
     7981 cccgtcgtcg agggtgccgg cggcatcgcc gatgatggcg aagtcctgga agtcggtctg
     8041 cgcctgctcc accagccggg tgcgctcctc accgcccacc ggtggcaggt tgcgcttgcg
     8101 gaccgccatg gcgcggtctc ggaagcgggc gagcattgcg tcgatgtcga gcgagtcggc
     8161 catggcccaa cgctaccgcc aggatcggcc gccgccggtc gatcgaggtc agtcggcggt
     8221 gatgccgagc gccttgcggc gcgcggccac cccggggcgc gccagggacg tcccgccggg
     8281 gatctccacg cgctggtgct cgccggtccg cgactcccag tcggcgtcgg cgctggcgtg
     8341 gtcggtgccg ggggcggccc ccagggcgac ctcgggcacg gcgacgggga cgacgggttg
     8401 cacggggtct gccttcggcg gcagctcggc gatgaacacg tcgtcgtctt cgacgtcgac
     8461 gtcgtcggcc aggaagggca ggtcggagat gtcgccctgg gcgaccggct tcgtcagccg
     8521 gatgtaccgg acggtcaaca agaggatcgc caacgccacg agcaccaggc cgccgatgat
     8581 ggcggccacc ttgcggctct cggtgaagat cggcttctcg ggtgcgtcct cggactcgac
     8641 cagggtctgg ttggtggagt cggtcgctgc ggtggtggcg gtcggggcgg aggtggatcc
     8701 ggcgaccgtg gtggcggtct cgccggtgcc gaacgtggca cccgggtcct gtgccggagc
     8761 tgcggtcgcc agcgccgccg gcgccacggc cgcgctgaat ccgagcgcga gtacgaacag
     8821 gagggtgcga cgtcggattg ccacgaagag gagagattac tggtcaggcc gtcgcagccc
     8881 gtgcacaggg ccggcaccag tacgtggtgc gtccgccgac ggtgcctcgt tcgagcggcg
     8941 tgccgcagcg agggcagtgg ccgtcgcggt ggcgctcgac ctgcaggtcc ccccggtggg
     9001 agccgccgcg ctcgaacagc tgccccacgg tcgaaccgat gcgccgctgc agccggcgca
     9061 gctcggcgcc ggtgagcgat cctgcctcgc gggtggggtc caggcgcgcc cgccacaggg
     9121 tctcgtccac cagcaggttg ccgagtccgg cgatgcggga ctggtccagc agccgcgcct
     9181 tcaacggtgc gcg
//
LOCUS       DATMFS010000551         3211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_113528, whole genome shotgun sequence.
ACCESSION   DATMFS010000551 DATMFS010000000
VERSION     DATMFS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113528"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VNQ33_10425"
     CDS             <1..182
                     /locus_tag="VNQ33_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98564.1"
                     /translation="GIDQRHRTARTLRPKLVCELTFYLGHHTDFVDRPCGAHRVRQMR
                     TNGLAAAVEPATLPP"
     gene            179..1096
                     /locus_tag="VNQ33_10430"
     CDS             179..1096
                     /locus_tag="VNQ33_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWJ98565.1"
                     /translation="MKPPAGSLARQARRQHGIFTGEQAVQHGVTAPALRNLLDAGWCQ
                     RLGRGVFAVTAAPRTREQALLAATLVHQAPAAASHRAAADLVGVPGYASVPPEVTVGH
                     GRNRRTVLGIVHGSLLLPDRHVTVRNGIAVTTIARTVFDLAGIEPEDKVGRALDFAIS
                     RRMCTLRQVNQVFFALAGQGRRGTVAMRTLLEKRGEGYMPPASELERLGRKVFGEGGL
                     PVPEFEIELGDGDLIGRVDCYWRAARLVVELDSGRFHDGLSARESDRRRDNRLMAAGW
                     RVLRFTWDDLVNRPVETVATIRAALARRP"
     gene            complement(1257..2000)
                     /locus_tag="VNQ33_10435"
     CDS             complement(1257..2000)
                     /locus_tag="VNQ33_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ98566.1"
                     /translation="MSTDPTLASASNASAASSSPRVLVVDDEEHITELLAMALGYQGF
                     DVERAATGREALDAVVRRAPDLMILDVMMPELDGFEVARRLRLDEGAATRIPIIFLTA
                     KDATQDKVQGLRLGSDDYVTKPFSIEELVERVKAVLRRSSGLGGGESKLTFADLELDE
                     DTREVRRDGRLIELTPTEYKLLRYLLANARRVLTRDQILEAVWDYTFAGNASVLETYI
                     SYLRQKVDATDPPLIHTVRGVGYSLRLPR"
     gene            2113..>3211
                     /locus_tag="VNQ33_10440"
     CDS             2113..>3211
                     /locus_tag="VNQ33_10440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ98567.1"
                     /translation="MSIDTDPATVPLPATPRRLPTAVTGADALFGTRPGSAPRLDPRH
                     PAGRGDHVVDQQFLAASEYRRRTAAVEIVIPVFNEEAVLDGSVRALHDYLADRFPLPW
                     LITVADNASTDDTWAHAAALHAELPDVQALRLSAKGRGRALRAAWSQSPAPVVAYMDV
                     DLSTGLDALLPLVAPLLSGHSDVAIGTRLAAGSHTTRGPKREAISRTYNLILRTTLRS
                     GFSDAQCGFKAIRREAVTELLPLVEDDGWFFDTELLVLAEHNGLRIHEVPVDWVDDPD
                     SSVDIIRTAADDLKGVGRMLRRFARGQGWIERRSLPAPAADRRELGGQLVRFGSVGVA
                     STLLFSVLFAALYGPIGATAATIGALAISSAA"
BASE COUNT          444 a         1149 c         1154 g          464 t
ORIGIN      
        1 agggaatcga ccagcgccac cggacggccc gcacccttcg accgaagttg gtgtgcgaac
       61 tgacgttcta cctcggtcac cacaccgact tcgtggaccg accgtgcggg gcacatcggg
      121 tgcgccagat gcgtaccaac gggttggccg cagcggtgga gccggccacc ctgccgccgt
      181 gaagccgccc gctggatcgc tcgccaggca agcacggcgc cagcacggca tcttcaccgg
      241 tgaacaagcc gtgcagcacg gcgtgacggc gcccgcactc cggaacctgc tcgacgcggg
      301 ctggtgccag cggctcgggc gcggcgtgtt cgccgtgacc gctgcaccca ggacccggga
      361 acaggcgctc ctcgccgcga cgctcgtgca ccaggcaccg gcggcggcgt cgcaccgggc
      421 ggcggccgat ctcgtgggcg tacccggcta tgcctcggtg ccgcccgagg tgaccgtcgg
      481 gcacggccgg aaccgtcgga cggtgctcgg gatcgtgcac ggttcgctgc tgctccccga
      541 tcgccacgtc acggtccgca acggcatcgc ggtcaccacg atcgcccgga cggtgttcga
      601 tctcgccggg atcgaaccgg aggacaaggt cggccgagcg ctcgacttcg ccatcagccg
      661 gaggatgtgc acgcttcgcc aggtgaacca ggtgttcttc gcgctcgccg gacagggtcg
      721 ccgaggcacg gtggcgatgc gcacgctcct cgagaagcgg ggcgagggct acatgccgcc
      781 ggccagcgag ctcgagcggc tcggccgcaa ggtgttcggc gagggaggcc tgccggtgcc
      841 cgagttcgag atcgagctcg gtgacggcga cctgatcggc cgtgtcgact gctactggcg
      901 agcggcgcgg ctggtggtcg agctcgacag cggccggttc cacgacggcc tgagcgcccg
      961 ggaatcggat cggcggcgag acaaccggtt gatggcagcc ggatggcggg tcctccgctt
     1021 cacctgggac gacctcgtca accgtccggt cgagacggtc gcgacgatcc gcgccgcgct
     1081 cgcacgtcgc ccctgaagct gcgcacccac ccggccacca ccggtgacca ctggtgaccg
     1141 ccctcaccga ggttggtgtg cgaactgatg tcgtccctcg gttttcacac caacttcggt
     1201 caggcgaccg ggtggtcagg cggccgggtg gtcgggtggt cgggaccggg ccgtggctac
     1261 ctggggaggc ggagggagta gccgacgcct cgcacggtgt ggatgagggg cgggtcggtg
     1321 gcgtcgacct tctggcggag gtagctgatg taggtctcca ggacgctggc gttgccggcg
     1381 aacgtgtagt cccacacggc ttcgaggatc tggtcgcggg tgaggacgcg gcgggcgttg
     1441 gcgagcaggt agcggaggag cttgtactcg gtgggggtga gctcgatgag gcggccgtct
     1501 cgccgcacct cgcgggtgtc ctcgtcgagc tccaggtcgg cgaaggtgag cttcgactcc
     1561 ccgccgccga gacccgacga ccggcggagg accgccttca cccgctccac gagctcctcg
     1621 atgctgaacg gcttggtcac gtagtcgtcg ctgccgaggc ggaggccctg gaccttgtcc
     1681 tgggtggcgt ctttggcggt gaggaagatg atcgggatgc gggtggcggc gccttcgtcg
     1741 aggcggagcc ggcgggcgac ctcgaagccg tcgagctcgg gcatcatcac gtcgaggatc
     1801 atcaggtcgg gagcccggcg gacgacggcg tcgagcgcct cgcggccggt cgccgcccgc
     1861 tcgacgtcga agccctggta gccgagcgcc atggcgagca gctcggtgat gtgctcctcg
     1921 tcgtcgacca ccaggacgcg gggcgatgac gacgcagcgg aggcgttcga ggcggaggcg
     1981 agcgtcgggt ccgtggacat ggaggccacc gtacgggcgc cccgtcgatc ggggaagggc
     2041 ctgcgccgat gtcccaggga actcccagga acggtggagt cgaggtggag ccgaccgtcc
     2101 cacgatcggc ccatgagcat cgacaccgat cccgccaccg tcccgctccc ggcgacgccc
     2161 cgccgcctcc cgaccgccgt gaccggtgcc gatgccctct tcggcacccg gcctggctcg
     2221 gcgccgcggc tcgacccccg tcaccccgcc ggccgaggcg accacgtggt cgaccagcag
     2281 ttcctcgccg cctcggagta ccggcgccgc acggctgccg tcgagatcgt catcccggtg
     2341 ttcaacgagg aggcggtcct cgacggctcg gtccgggcgc tgcacgacta cctcgccgac
     2401 cgcttccccc tcccctggct catcaccgtc gccgacaacg cctccaccga cgacacctgg
     2461 gcccacgccg ccgccctcca cgccgagctg cccgacgtcc aggcactccg cctcagcgcc
     2521 aaggggcggg ggcgggcgct gcgtgccgcg tggtcccaga gcccggcgcc ggtggtggcg
     2581 tacatggacg tcgacttgtc gaccggcctc gacgccctcc tgcccctggt cgctcccctg
     2641 ctgagcggcc acagcgacgt ggccatcggg acccggctgg cggccggctc gcacaccacc
     2701 cgggggccga agcgcgaggc gatctcccgc acctacaact tgatcctgcg cacgacgctg
     2761 cgcagcgggt tctccgacgc ccagtgcggg ttcaaggcca tccggcgcga ggccgtcacc
     2821 gagctgctcc cgctggtcga agacgacggc tggttcttcg acaccgagct gctggtcctc
     2881 gccgagcaca acggcctgcg gatccacgag gtgcccgtgg actgggtcga tgatcccgac
     2941 tccagcgtcg acatcatccg cacagcggcc gatgacctca agggcgtggg ccggatgctc
     3001 cggcgcttcg cccgcggcca ggggtggatc gagcgccgga gcctgccggc accagccgcg
     3061 gaccgccggg agctcggcgg ccagctcgtc cgcttcgggt ccgtcggggt ggccagcacc
     3121 ctgctcttct cggtgctgtt cgctgccctc tacggcccca tcggcgccac ggcggcgacg
     3181 atcggcgccc tcgccatctc atcggcggcg a
//
LOCUS       DATMFS010000552         8332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_88739, whole genome shotgun sequence.
ACCESSION   DATMFS010000552 DATMFS010000000
VERSION     DATMFS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88739"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VNQ33_10445"
     CDS             complement(<1..360)
                     /locus_tag="VNQ33_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWJ98568.1"
                     /translation="MSVSPILGPMGPLVTDHRLTTLLGDWSADGHGTLARRLAQGLRK
                     AIVGGLLPDGARLPAERHLAELLSVSRSTVTTALDELRSEGLLRSQQGRGTEVVGPGE
                     VMTGHRVGAHFVSRGVGI"
     gene            complement(597..1562)
                     /locus_tag="VNQ33_10450"
     CDS             complement(597..1562)
                     /locus_tag="VNQ33_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98569.1"
                     /translation="MKDIRVGFVGIVVAGLLVGTGCSSAGSETGAGSGKGATTRPAPT
                     GSTTAPAASTVPEPLADQTPPSTINGLTLQGDTLWVASIEDDEVLQVDADSGAILRRI
                     DTHGAGPDDVAVAPDGSVWTAGFANGDLGRIDPDGTYQVVHHIEVGLNPIEFGSDGML
                     YVGTSGKLGKLYALITDGGAPVAGFSPKAVVQWLPDINAFAVLDDGTLVAPAGGLTGP
                     SGAVRIDPAKGTVTPIVTGLPAVAAGAADAHGVPYLLANITGEIIRIDVEAKTSEVVE
                     TVRKGVPFDNLAFAPDGTVYVSSFVDPTITVVAPDGVERVLSIGS"
     gene            1837..5466
                     /locus_tag="VNQ33_10455"
     CDS             1837..5466
                     /locus_tag="VNQ33_10455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWJ98570.1"
                     /translation="MSSRSTIRDRYSFANLEEVLPLPDLIAIQRESFDWFLEQGLADT
                     FRDISPIKDFTEALSLHLEFDPTDEDLRPTPKFTVEECKERDMTYSAPIFVRASFQNA
                     NTGEIKEQTVFMGDFPKMTSKGTFVINGTERVVVSQLVRSPGVIFQPGERFRLRNLAK
                     HQLVTGTIHPYRGEWIEFDVEQKPGKDVTAGTRVARKRRLGIFVLLRALGYDEENAPG
                     FLDAFVRHFDFLEGQWEKERDIAPTQEEALVEIYKRARPGEPPSVESAKAYFRNAFFE
                     SRRYDLSRVGRYKLDRKLGHEIDKIAELFPMLEGRTLVSDDVTPLLDRPEPGQPVLSR
                     CEVLAACTYLLHLVQATPGYRLDDQDHFANRRIRSVGELIQNQVRIGLSRMERVVRER
                     MTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQVNPLSGLTHRRRLSALGPG
                     GLSRERAGFEVRDVHFSHYGRMCPIETPEGPNIGLIGALATYARVNEFGFIESPYRKV
                     ENGKVTDEIMYLAADEEEEYVVAQANTPVNPDGSFAQERVLVRRSPQAASLEDLRLQL
                     ERDIFFGATTDIQYVPGSEVQLMDVSPKQIVSIGAALIPFLEHDDANRALMGANMQKQ
                     AVPLVKAEAPYIGTGVEGRAARDAADMIIATDDGEITEVDGTLIKVAYKSQGNVTYKL
                     NKFERSNQDTCINQRPIVAAGEKISAGDILANGPSTDNGELALGKNLVVAFMTWEGYN
                     FEDAIILSERLVKDDVLTSIHIKEHEIDARDTKLGPEEITRDIPNLSEDILADLDERG
                     IIRIGAEVEAGDVLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGK
                     VIDVRVQTREDGHELPPGVNQLVKVYVGQRRKISVGDKLAGRHGNKGVISRILPIEDM
                     PFTADGSPVDIILNPLGVPSRMNVGQVMESHLGYAARWGWDHEKSGKVGDEPWRTERK
                     TRPATPPAKLIATPVFDGAFWDEENRTKHPTIQQILTNLTPESPYPEYGDGGRLIQAN
                     GKTTLYNGRTGEAYDNPITVGYMYILKLAHLVDDKIHARSTGPYSMITQQPLGGKAQF
                     GGQRFGEMEVWALEAYGAAYCLQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIPESF
                     KVLIKEMQALCLNVEVLAVDGAEIEMRELDEDVFRTAEELGIDLSRPERGSDEEDERR
                     RAERA"
     gene            5501..>8332
                     /gene="rpoC"
                     /locus_tag="VNQ33_10460"
     CDS             5501..>8332
                     /gene="rpoC"
                     /locus_tag="VNQ33_10460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWJ98571.1"
                     /translation="MLDVNNFDQLRIGLATADSIRMWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECYCGKYKRVRFKGIICERCGVEVTRSKVRRERMGHIELAAPTV
                     HIWYLRGTRSWLAYLLMGTEPREELKAKQLEKVIYFAANLVVSVDEEKRDQDLPSLEA
                     EVLAEREAIESERELELARRMEELEGEIAGLESEGAKDADIKARQKQADKDLAMIRER
                     YELELDVLNRAFDEFKGLFSRQIIEDEMLWRELEDRYGEYFEGGMGADAIKSLIAKLD
                     FDEEEVKLRAAIDPQEGQRPLSAQRKQKAIKRLKIVSSFNQRDENGKRINDPGAMILD
                     VVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRM
                     LQEAVDALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGPTLKLHQCGLPKLMALELFKPFVMKKLVDAELAQNIKSAKRMVERRRPQVWDVLEE
                     AIKEHPVMLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCTAFNADFDGDQMAVHLPL
                     SSEAQAEARVLMLSANNVLSPAHGRPLVTPTQDMIIGAFYLTEVVEGGAGEGRVFRHR
                     YEVERAYESGDLALHSRIQFRGLDADGERIIAEDTTAGRLFFNRALPDSFEYMNKRIA
                     KKEMGEIVDRLARHEAKATVSSSLDAIKNLCFRYAAQSGITISIDDVKTPPEKAGIME
                     DHELQAQKVEDQFRRGIITDGERRQKEVEIWTDATDMVRTAMEKNLKNEQFNPIEMMV
                     GSGARGNMMQVRQIAGMRGLVANPRGDMIPRPIKSNFREGLSMLEYFIATPGARKGLV
                     DTALRTADSGYLTRRLVDVAQELIINDEDPFAKGGPVRGVWLEGIEPDAPGRRSYLET
                     RLFSRTLSDDVTLSDGTVIEKGTMVDMDLM"
BASE COUNT         1441 a         3001 c         2714 g         1176 t
ORIGIN      
        1 gatccccacg ccccggctca cgaagtgggc gccgacccgg tggccggtca tgacctcgcc
       61 gggccccacc acctcggtgc cgcggccctg ctgcgaccgc aggaggccct cgctgcgcag
      121 ctcgtcgagg gcggtggtga ccgtgctgcg gctgacgctc aggagctcgg ccaggtggcg
      181 ttcggcgggc aggcgggcgc cgtcgggcag gaggccgccc acgatggcct tgcgcagccc
      241 ctgcgccagc cgccgggcga gcgtgccgtg cccgtcggcg gaccagtcgc cgagcaaggt
      301 ggtgaggcgg tggtcggtga cgagcggccc cattggcccg aggattggac tgacggacat
      361 cccgccaatc tggtgggatt ggcccgtgtt gtccagggcc aatcggcgca gattggaccc
      421 atgttcgctc cctggtgccc cgtctgcgag tcccggatgc tcctcggccc ccgccgtgtg
      481 gcgtcgatcg agcgcggtcc cagcggcgtt cgcgcggtgc tccggtgcca ctgcggcacc
      541 ctgctggtct ggtcgccgga gggcgagggc agcgagggcg gtgcacccgc cgagggtcag
      601 gagccgatcg agagcacccg ttcgacgccg tcgggcgcca cgacggtgat ggtcgggtcg
      661 acgaagctcg acacgtagac cgtgccgtcg ggcgcgaagg ccaggttgtc gaacggcacc
      721 cccttcctca ccgtctcgac cacctccgag gtcttggcct cgacgtcgat gcggatgatc
      781 tcgccggtga tgttggcgag caggtacggc acgccgtggg cgtcggcggc cccggcggcg
      841 accgcgggca gaccggtgac gatgggggtg accgtgccct tcgccgggtc gatgcgcacg
      901 gcgccgctcg gcccggtgag gccgcccgcg ggcgccacga gcgtgccgtc atcgagcacg
      961 gcgaacgcgt tgatgtccgg cagccactgc accaccgcct tcggtgagaa cccggcgacg
     1021 ggcgcgccgc cgtcggtgat cagggcgtac agcttcccga gcttgccgga ggtgcccacg
     1081 tagagcatgc cgtcggagcc gaactcgatc gggttgaggc cgacctcgat gtggtgcacc
     1141 acttggtagg tcccgtcggg gtcgatgcgc ccgaggtcgc cgttggcgaa cccggcggtc
     1201 cacacggacc cgtcgggcgc gaccgcgacg tcgtcggggc cggcgccgtg ggtgtcgatg
     1261 cgccggagga tggcgccgct gtcggcgtcg acctgcagca cctcgtcgtc ttcgatgctg
     1321 gcgacccaca gcgtgtcccc ctgcagggtg agcccgttga tggtcgatgg cggggtctgg
     1381 tccgcgagcg gctcggggac cgtggaggcg gccggggcgg tcgtggaccc ggtcggcgcc
     1441 gggcgcgtcg tcgcgccctt cccgctcccg gctcccgtct ccgagcccgc cgacgagcac
     1501 cccgtgccga ccaggagccc agccaccacg atcccgacga acccgacccg gatgtccttc
     1561 atgacgggcc aagctacggc gggagcgcgg cggcgctgcg gaccggcgcc gcgccagcag
     1621 gtccttgacc gatcgggggg ttcggtttac cgtcaccgca acccagacgg cccccgcccg
     1681 gatccatgca ggtccgggtt gtgttcgcgt tgcaggcatc gctaggatcg cccttcgccg
     1741 tggtgcagcg tgctggctct cagaaatggt tgatcgaagc gacgcgccct gccaccggcc
     1801 ttcgtcgcgt gatcaccagc tgctgaggag cctgcgttgt cctctcgttc caccatccgg
     1861 gaccgttact cgttcgcgaa cctggaggag gtcctgcccc ttcccgatct gatcgccatc
     1921 cagcgcgaat cgttcgactg gttcctggag cagggcctcg ccgacacgtt ccgcgacatc
     1981 tcgccgatca aggacttcac cgaagcgctg tcgctgcacc tggagttcga tcccaccgac
     2041 gaggacctgc gccccacgcc gaagttcacg gtcgaggagt gcaaggagcg ggacatgacc
     2101 tactccgcgc cgatcttcgt ccgggccagc ttccagaacg ccaacacggg cgagatcaaa
     2161 gagcagacgg tcttcatggg ggacttcccc aagatgacct ccaagggcac cttcgtcatc
     2221 aacggcaccg agcgcgtcgt cgtgtcgcag ctcgtccgct cgcccggcgt catcttccag
     2281 cctggtgagc gcttccgcct ccggaacctg gccaagcacc agctcgtcac cggcaccatc
     2341 cacccctacc gcggcgagtg gatcgagttc gacgtcgagc agaagcccgg caaggacgtc
     2401 accgccggca cccgcgtcgc ccgcaagcgc cgcctcggca tcttcgtgct gctgcgcgcc
     2461 ctcggctacg acgaggagaa cgcccccggc ttcctcgacg ccttcgtccg ccacttcgac
     2521 ttcctcgagg gccagtggga gaaggagcgc gacatcgcgc ccacccagga agaggcgctc
     2581 gtcgagatct acaagcgggc ccgcccgggc gagccgccgt cggtcgagtc ggccaaggcc
     2641 tacttccgca acgccttctt cgagtcccgc cgctacgacc tcagccgggt cggccgctac
     2701 aagctcgacc gcaagctcgg ccacgagatc gacaagatcg ccgagctgtt cccgatgctc
     2761 gagggccgca cgctcgtctc cgacgacgtg accccgctgc tcgaccgtcc cgagcccggc
     2821 cagccggtgc tcagccgctg cgaggtcctc gccgcgtgca cctacctcct gcacctcgtc
     2881 caggccaccc ccgggtaccg cctcgacgac caggaccact tcgccaaccg ccgcatccgc
     2941 tcggtgggcg agctcatcca gaaccaggtc cgcatcgggc tgtcccgcat ggagcgggtg
     3001 gtgcgcgagc gcatgaccac ccaagacgtc gaggccatca cgccccagac gctgatcaac
     3061 atccgccccg tggtcgcggc catcaaggag ttcttcggga cctcccagct gtcgcagttc
     3121 atggaccagg tgaacccgct gtcgggcctc acccaccgcc gccgcctctc ggcgctcggc
     3181 ccgggcggcc tctcgcgcga gcgggccggc ttcgaggtcc gcgacgtgca cttcagccac
     3241 tacggccgca tgtgcccgat cgagacgccc gaaggcccga acatcggcct gatcggcgcc
     3301 ctcgccacct acgcacgggt caacgagttc ggcttcatcg agtcgcccta ccgcaaggtg
     3361 gagaacggca aggtcaccga cgagatcatg tacctcgccg ctgacgagga ggaggagtac
     3421 gtcgtcgccc aggcgaacac gccggtcaac cccgacggct cgttcgccca agagcgcgtg
     3481 ctcgtgcgcc gctcccccca ggccgcctcg ctcgaggacc tgcgcctgca gctcgagcgc
     3541 gacatcttct tcggtgccac tacggacatc cagtacgtgc ccggcagcga ggtccagctc
     3601 atggacgtct cgcccaagca gatcgtctcc atcggcgccg ccctgatccc gttcctcgag
     3661 cacgacgacg ccaaccgggc gctcatgggc gccaacatgc agaagcaggc ggtgccgctg
     3721 gtcaaggccg aggcccccta catcggcacc ggtgtcgagg gccgtgcggc ccgcgacgcg
     3781 gccgacatga tcatcgccac cgacgacggc gagatcaccg aggtcgacgg cacgctcatc
     3841 aaggtggcgt acaagtccca gggcaacgtc acctacaagc tcaacaagtt cgagcggtcc
     3901 aaccaggaca cgtgcatcaa ccagcggccc atcgtcgccg ccggggagaa gatcagcgcc
     3961 ggcgacatcc tcgccaacgg ccccagcacc gacaacggcg agctggccct cggcaagaac
     4021 ctggtcgtgg ccttcatgac ctgggagggc tacaacttcg aggacgccat catcttgtcg
     4081 gagcgcctgg tgaaggacga cgtgctcacg tcgatccaca tcaaagagca cgagatcgac
     4141 gcccgcgaca ccaagctggg cccggaggag atcacccggg acatcccgaa cctgagcgag
     4201 gacatcctcg ccgacctcga cgagcgcggg atcatccgca tcggcgccga ggtcgaggcc
     4261 ggcgacgtgc tggtcggcaa ggtcacgccc aagggcgaga ccgagctcac gcccgaagag
     4321 cgcctgctgc gcgccatctt cggcgagaag gcccgtgagg tccgcgacac ctcgctgaag
     4381 gtgccccacg gcgagaccgg caaggtcatc gacgtccggg tccagacccg cgaggacggc
     4441 cacgagctgc cccccggcgt caaccagctg gtgaaggtct acgtcggcca gcgccgcaag
     4501 atcagcgtcg gcgacaagct cgccggccgc cacggcaaca agggcgtcat ctcccggatc
     4561 ctccccatcg aggacatgcc gttcacggcc gacggctcgc ccgtcgacat catcctgaac
     4621 cccctcggcg tcccgtcccg catgaacgtg ggccaggtca tggagtcgca cctcggctac
     4681 gccgcccgct ggggctggga ccacgagaag tccggcaagg tcggcgacga gccgtggcgc
     4741 accgagcgca agacccgtcc cgccaccccg ccggccaagc tcatcgccac cccggtgttc
     4801 gacggtgcct tctgggacga ggagaaccgc accaagcacc ccacgatcca gcagatcctg
     4861 accaacctca ccccggagtc cccgtacccc gagtacggcg acggcggccg gctgatccag
     4921 gcgaacggca agaccaccct ctacaacggg cgcaccggcg aggcgtacga caacccgatc
     4981 accgtcgggt acatgtacat cctgaagctg gcccacctcg tggacgacaa gatccacgcc
     5041 cgctccaccg gcccctactc catgatcacc cagcagccgc tcggtgggaa ggcccagttc
     5101 ggtggccagc gcttcggcga gatggaggtg tgggccctcg aggcctacgg cgccgcctac
     5161 tgcctgcagg agctgctgac catcaagtcc gacgacgtgc tcggccgcgt gaaggtctac
     5221 gaggccatcg tcaagggcga gaacatcccc gaacccggca tccccgagtc cttcaaggtg
     5281 ctcatcaagg agatgcaggc cctgtgcctg aacgtcgagg tcctggccgt cgacggcgcc
     5341 gagatcgaga tgcgcgagct cgacgaggac gtcttccgca ccgcggaaga gctcggcatc
     5401 gacctctccc ggcccgaacg agggtcggac gaagaagacg agcgccgccg cgctgagcgg
     5461 gcctgaccgg atcgactgag agatagagga acgagacaac atgctcgacg tcaacaactt
     5521 cgaccagctc cggatcggcc tggccaccgc ggactccatc cgcatgtggt ccaacggcga
     5581 ggtcaagaag ccggagacca tcaactaccg cacgctcaag ccggagaagg acgggctctt
     5641 ctgcgagaag atcttcggtc cgaccaagga ctgggagtgc tactgcggca agtacaagcg
     5701 ggtgcggttc aagggcatca tctgcgagcg ctgcggcgtg gaggtcaccc gctccaaggt
     5761 ccggcgcgag cgcatgggcc acatcgagct ggccgccccc accgtccaca tctggtacct
     5821 ccgcggcacc cggtcgtggc tggcctacct gctgatgggc accgagcccc gtgaggagct
     5881 gaaggccaag cagctggaga aggtcatcta cttcgccgcc aacctcgtcg tctccgtcga
     5941 cgaggagaag cgcgaccagg acctgccgtc cctcgaggcc gaggtgctcg ccgagcgcga
     6001 ggccatcgag tccgagcgcg agctggagct ggcccgccgg atggaagagc tcgagggcga
     6061 gatcgccggg ctcgagtccg aaggggccaa ggacgcggac atcaaggccc gccagaagca
     6121 ggccgacaag gacctcgcca tgatccgcga gcgctacgag ctggagctcg acgtgctcaa
     6181 ccgggcgttc gacgagttca agggcctctt cagccgccag atcatcgaag acgagatgct
     6241 gtggcgcgag ctcgaggacc gctacggcga gtacttcgag ggcggcatgg gcgccgatgc
     6301 gatcaagtcg ctcatcgcca agctcgactt cgacgaggaa gaggtcaagc tgcgggccgc
     6361 catcgacccg caggagggcc agcgccccct gtcggcgcag cgcaagcaga aggccatcaa
     6421 gcgcctgaag atcgtctcgt cgttcaacca gcgcgacgag aacggcaagc gcatcaacga
     6481 cccgggggcc atgatcctcg acgtcgtccc ggtgatcccg ccggagctgc gcccgatggt
     6541 ccagctcgac ggcggccgct tcgccacctc ggacctcaac gacctgtacc gccgggtcat
     6601 caaccgcaac aaccgcctga agcgcctgct cgacctcggg gctcccgaga tcatcgtgaa
     6661 caacgagaag cgcatgctcc aggaggccgt cgacgccctg ttcgacaacg gccgccgcgg
     6721 tcgaccggtc accggaccgg gcaaccgccc gctcaagtcg ctgtccgaca tgctcaaggg
     6781 caagcagggc cggttccgcc agaacctgct cggcaagcgc gtcgactact cgggccgttc
     6841 ggtcatcgtg gtcggcccga cgctcaagct gcaccagtgc ggcttgccca agctcatggc
     6901 gctggagctc ttcaagccct tcgtgatgaa gaagctcgtc gacgccgagc tggcgcagaa
     6961 catcaagtcg gccaagcgca tggtcgagcg ccgtcgcccg caggtgtggg atgtcctcga
     7021 agaggccatc aaggagcacc cggtcatgct caaccgtgcc cccacgctcc accgcctggg
     7081 catccaggcg ttcgagccgg tgctggtcga gggcaaggcc atccagatcc acccgctggt
     7141 gtgcaccgcc ttcaacgccg acttcgacgg cgaccagatg gccgtccacc tcccgctgtc
     7201 gtcggaggcg caggccgagg cccgcgtgct gatgctgtcg gccaacaacg tgctgtcgcc
     7261 cgcccacggg cgtccgctgg tcacgcccac ccaggacatg atcatcggcg ccttctacct
     7321 caccgaggtg gtcgagggcg gcgcgggcga aggccgcgtg ttccgccacc gctacgaggt
     7381 cgagcgggcc tacgagtccg gtgacctggc gctgcactcg cggatccagt tccgtgggct
     7441 cgacgccgac ggcgagcgca tcatcgccga ggacaccacg gccggccgcc tgttcttcaa
     7501 ccgggccctc ccggacagct tcgagtacat gaacaagcgc atcgccaaga aggagatggg
     7561 cgagatcgtg gaccggctcg cccgccacga ggccaaggcc accgtgtcga gcagcctcga
     7621 cgccatcaag aacctgtgct tccgctacgc ggcccagtcc ggcatcacca tctccatcga
     7681 cgacgtgaag acgccgccgg agaaggccgg gatcatggag gaccacgagc tccaggccca
     7741 gaaggtcgag gaccagttcc gccggggcat catcaccgat ggtgagcgcc gccagaagga
     7801 ggtggagatc tggaccgatg ccaccgacat ggtgcgcacc gcgatggaga agaacctcaa
     7861 gaacgagcag ttcaacccca tcgagatgat ggtgggctcg ggtgctcgcg gcaacatgat
     7921 gcaggtccgc cagatcgccg gcatgcgcgg gctcgtggcc aacccccgcg gcgacatgat
     7981 cccgcgcccc atcaagagca acttccgtga aggcctctcg atgctggagt acttcatcgc
     8041 cacgccgggt gcccgcaagg ggctcgtcga cacggccctg cgcaccgcgg actcgggcta
     8101 cctcacccgt cgcctcgtcg acgtggccca ggagctgatc atcaacgacg aggacccctt
     8161 cgccaagggc ggccccgtcc gcggtgtgtg gctcgagggc atcgagcccg acgcccccgg
     8221 gcgccggagc tacctcgaga cgcgcctgtt ctcgcggacc ctctccgacg acgtgaccct
     8281 ctccgacggc accgtcatcg agaagggcac catggtcgac atggacctca tg
//
LOCUS       DATMFS010000553         4776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_135182, whole genome shotgun sequence.
ACCESSION   DATMFS010000553 DATMFS010000000
VERSION     DATMFS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135182"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VNQ33_10465"
     CDS             complement(<1..1060)
                     /locus_tag="VNQ33_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWJ98572.1"
                     /translation="MTPSPAPAARRPVRRALVGLTAVGLLGLAACGAPPTTTGSGSGR
                     NAEGSEAKIDPKDCPISALDEAKGPVKVNLWFSGLVDPALGVLQDMAKRYNASQDKVV
                     VTTNNQGNAYQEGLAKYRGASATPAQLPQIQYLEDTMLGELVDKGQVLPAEACMEADG
                     FDPTQITPAARAAYSVDDVLYPGYMNVSTPILYYNKVHFQKAGLDPDNPPKTLKEIEE
                     TSKKLKAAGVSKKPFSFKADQWFISTWLSGVGEKMVNNNNGRTKPASEATFDSDAAKE
                     VFGDLQRMDEEGLLNAFPVTDGSIDHYLALINEDSSMVVETSTASGTIAAALGGELAN
                     GTGGVKIDTSALDGKKIVP"
     gene            complement(1082..2335)
                     /locus_tag="VNQ33_10470"
     CDS             complement(1082..2335)
                     /locus_tag="VNQ33_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWJ98573.1"
                     /translation="MATSDVKSQGAAELAMGQSPARKNAGLVGQYLTLGLLSLLVLGP
                     IGLTVIQALSPPFRYVSAGRPLHPVEVSWQDRTWFSGGPLSLVVRTLIVFALLAWLQK
                     VASGGGWKDKAYLVPSRIVAIVGGTVALALVMNPLFQSFFKADGNTAVLYAAAVVAVA
                     ATQVWAYLSPARSPITSVVMALFTGFGLISFCIVTMGPTVWTLAWTRYDLGPAMGRSF
                     VMTICVTVLQVSTAIMAAYAFVFLRFPFKRILFALFMATLLLPLEVTLVGNVATIRQL
                     DWTDTMKALVLPFGAAALGTFLIRQGFRGLPTEIQDATRLDGYGHVAFLTRFAVPLTR
                     PVIAAFTVISALSAWNQYLWPRSITESNSFNTLQIQLRLAAASEVPNANQAIAAALVA
                     AVPVVLLLIAFQRQIIRGLTAGAVK"
     gene            complement(2322..3242)
                     /locus_tag="VNQ33_10475"
     CDS             complement(2322..3242)
                     /locus_tag="VNQ33_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWJ98574.1"
                     /translation="MSVTDATALPDGAPVVDAPTTSRRRGTYDAKEVGLALLFLVPSL
                     AVFAVFFYLPFARLLSWGTYESVRGGASYNQVGIDQYREVLGGHEFRIGLWHSLQFVL
                     WTVPFGLVLGTLLAVAAHRRLKGIKVFQAIFSSTLASSVAVSSVIFFFLFNPAVGFFR
                     VSWQSSPRWAIFAAALPAIWQNLGLSFVIVLAGLQAVPDEVMEAARLDGYGAWRRLTR
                     ITLPLIAPVLMFLLVILVIYGFQAFAQIDIITKGGPGGASETLVFKIFKQTQDYGKGA
                     VLSVGLFCVTLVVTFIQFAILDRRVHYGDE"
     gene            complement(3242..4345)
                     /gene="ugpC"
                     /locus_tag="VNQ33_10480"
     CDS             complement(3242..4345)
                     /gene="ugpC"
                     /locus_tag="VNQ33_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWJ98575.1"
                     /translation="MASVTFDAVSKRYGEVTAVDDLNLAIEDGEFMVLLGPSGCGKTT
                     ALRMIAGLEDISDGKLSIGDEVVNDVDPSRRDISMVFQSYALYPHMTVQRNIESPLLV
                     RSYHVDGNDKPARKLTKAERDERVAEAARVLDLEPYLKRKPAALSGGQRQRVALARAF
                     VGRPKVFLMDEPLSNLDAKLRAQTRIELVELHARVGTTVVYVTHDQVEAMTMATRVAV
                     MSMGKLQQVGTPQEVYARPVNTFVAQFIGTPPMNCFPGRISADGTRLEVEGGTLPLPA
                     GLVAVPAAGSTITVGVRPEHLQVSAEGSLGVEVRAVEWLGHECLISASIGDATVVVRE
                     AGMSASEAGSSLQLSVDPANVHLFDPNTTLRIG"
     gene            4555..>4776
                     /locus_tag="VNQ33_10485"
     CDS             4555..>4776
                     /locus_tag="VNQ33_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98576.1"
                     /translation="MVSSALADRSYRPSSDGFSIEPVAAQLYAAFASVAPSSPVVHCP
                     HCVSTGDLAALAVPPAAVPPETVSRFVRKT"
BASE COUNT          779 a         1576 c         1704 g          717 t
ORIGIN      
        1 cggggacgat cttcttgccg tccaaggccg acgtgtcgat cttcacgccg ccggtgccgt
       61 tggccagctc gccgcccagg gcagcggcga tggtgcccga ggcggtcgac gtctccacca
      121 ccatggagct gtcctcgttg atcagcgcca ggtagtggtc gatgctgccg tcggtcacgg
      181 ggaaggcgtt gaggaggccc tcctcgtcca tccgctggag gtcgccgaac acctccttgg
      241 cggcgtccga gtcgaacgtc gcctcggagg ccggcttggt gcggccgttg ttgttgttga
      301 ccatcttctc gcccacaccc gacagccaag tcgagatgaa ccactggtcg gccttgaagg
      361 agaacggctt cttggacacg ccggcggcct tcagcttctt ggaggtctcc tcgatctcct
      421 tcagcgtctt gggcgggttg tccgggtcca gcccggcctt ctggaagtgg accttgttgt
      481 agtagaggat cggcgtcgag acgttcatgt acccggggta gagcacgtcg tccacgctgt
      541 aggccgcccg ggccgcgggg gtgatctgcg tggggtcgaa gccatcggcc tccatgcatg
      601 cctcagccgg cagcacctgg cccttgtcca ccagctcgcc gagcatggtg tcctcgaggt
      661 actggatctg cgggagctga gcgggcgtgg ccgaggcccc gcggtacttc gccaacccct
      721 cctggtacgc gttgccctgg ttgttggtgg tgaccaccac cttgtcctgg ctggcgttgt
      781 agcgcttggc catgtcctgg aggacgccca gagccgggtc caccaggccg ctgaaccaca
      841 ggttgacctt gacggggccc ttggcctcgt cgagtgccga gatggggcag tccttgggat
      901 cgatcttggc ttcgctgccc tcggcgttgc ggccgctgcc ggagccagtg gtggtgggcg
      961 gcgcgccaca ggcggcgaga ccgaggaggc cgacggcggt gaggcccacc agggcccggc
     1021 ggacgggtcg tcgggcggcg ggagcgggag acggggtcat cgggaacctc tcaagagcgg
     1081 gtcacttgac ggcgccggcc gtcaggccac ggatgatctg acgctggaag gcgatcagga
     1141 gcaagacgac gggtacggcg gccacgagcg cggcggcgat ggcctggttg gcgttcggca
     1201 cctcactcgc tgccgccagc cggagctgga tctgcagggt gttgaaggag ttggactcgg
     1261 tgatcgatcg aggccagagg tactggttcc acgcgctcag ggcggagatc acggtgaagg
     1321 cggcgatcac cggccgggtg agcggcacgg cgaacctggt gaggaaggcg acgtggccgt
     1381 acccgtcgag gcgggtcgcg tcctggatct cggtgggcag cccccggaag ccctgccgga
     1441 tgaggaacgt gcccagcgcc gcggcgccga agggcagaac cagcgccttc atggtatcgg
     1501 tccagtccag ctggcggatg gtggccacgt tgccgacaag cgtgacctcg aggggcagca
     1561 gcagcgtcgc catgaacagc gcgaacaaga tgcgcttgaa cgggaaacgc aggaacacga
     1621 acgcgtacgc cgccatgatg gcggtgctca cctgcagcac cgtgacgcag atcgtcatca
     1681 cgaagctgcg tcccatggct ggcccgaggt cgtagcgggt ccacgccagc gtccagacgg
     1741 tggggcccat cgtcacgatg cagaaggaga tgaggccgaa cccggtgaac agggccatca
     1801 ccaccgacgt gatcggcgac cgagccgggg acaggtaggc ccacacctgg gtggccgcca
     1861 ccgccaccac ggcggcggcg tagagcacgg cggtgttgcc gtcggccttg aagaacgact
     1921 ggaacagcgg gttcatcacc agcgccaagg cgacggtccc gcccacgatg gcgacgatgc
     1981 ggctcggcac caggtacgcc ttgtccttcc atccgccacc cgacgcgacc ttctggagcc
     2041 aggccagcag ggcgaagacg atcagcgtcc gcaccaccag tgaaagcgga ccaccgctga
     2101 accaggtgcg gtcctgccag gacacctcga cggggtgcag cgggcggccg gcggagacgt
     2161 accggaacgg gggcgagagc gcctggatca ccgtcagccc gatcggcccg agcaccagga
     2221 gcgagagcag cccgagcgtg aggtactggc cgacgaggcc ggcgttcttg cgggcgggcg
     2281 actgccccat ggccaactcg gccgcgccct ggctcttcac gtcactcgtc gccatagtgc
     2341 acccgccgat cgaggatggc gaactggatg aacgtgacca ccagggtcac gcagaacagg
     2401 ccgactgaca gcacggcacc ctttccgtag tcctgcgtct gcttgaagat cttgaacacc
     2461 agcgtctcgc tggccccgcc gggaccgccc ttcgtgatga tgtcgatctg ggcgaacgcc
     2521 tggaacccgt agatcaccag gatcaccagc aggaacatca gcaccggagc gatgagcggg
     2581 agggtgatgc gggtgaggcg gcgccaggcg ccgtagccgt cgagccgcgc cgcttccatc
     2641 acctcgtcgg gcacggcctg gagcccggcg agcacgatca cgaaggacag gcccaggttc
     2701 tgccagatgg ccggcagggc ggcggcgaag atcgcccacc gtgggctgga ctgccagctg
     2761 acccggaaga agcccaccgc cgggttgaac aggaagaaga agatcaccga ggacacggcg
     2821 accgaactgg cgagcgtcga cgagaagatg gcctggaaga ccttgatccc cttgagccgt
     2881 cggtgagcag cgacggcgag gagcgtgccg agtacgagtc cgaacgggac ggtccacagc
     2941 acgaactgga ggctgtgcca gagcccgatg cggaactcgt ggccgcccag cacctcgcgg
     3001 tactggtcga tccccacctg gttgtaggag gccccgcccc gcacggactc gtacgtgccc
     3061 cagctgagga gccgggcgaa gggcaggtag aagaacaccg cgaacacggc gagcgacggg
     3121 accaagaaga gcagcgccag gccgacctcc ttggcgtcgt acgtgccgcg ccgccgcgac
     3181 gtcgtggggg catcgacgac gggcgcgccg tcggggagcg ccgtggcatc ggtgacgctc
     3241 atcagccgat ccgcagcgtc gtgttcgggt cgaagaggtg cacgttcgcc gggtcgacgg
     3301 acagctggag gctgctcccc gcctcgctgg ccgacatgcc ggcctcgcgg acgacgaccg
     3361 tggcgtcgcc gatcgacgcg gagatcaggc actcgtggcc gagccactcg accgcccgga
     3421 cctcgacacc gagcgagccc tcggccgaca cctgcaggtg ctcgggccgc acgccgacgg
     3481 tgatggtgga gccggccgcg ggcacggcga ccaggccggc cggaaggggc agcgtgccgc
     3541 cttcgacctc gagccgggtg ccgtcggcgg agatcctgcc cgggaagcag ttcatggggg
     3601 gcgtcccgat gaactgggcc acgaacgtgt tcacgggtcg ggcgtacacc tcctggggcg
     3661 tgcccacctg ctggagcttg cccatcgaca tgaccgcgac ccgggtggcc atggtcatgg
     3721 cctcgacctg gtcgtgggtc acgtagacca cggtggtgcc gacccgggcg tggagctcga
     3781 cgagctcgat gcgggtctgg gcccggagct tggcgtcgag gttcgacagc ggctcgtcca
     3841 tcaagaagac cttcgggcgg cccacgaacg cccgggcgag cgccacgcgc tggcgctggc
     3901 cgcccgagag cgcggccggc ttgcgcttca ggtacggctc gaggtcgagg acccgggcgg
     3961 cctcggccac ccgctcgtcg cgctcggcct tggtcagctt gcgagccggc ttgtcgttgc
     4021 cgtcgacgtg gtagctgcgc accagcagcg gggactcgat gttccgctgc accgtcatgt
     4081 gcgggtagag ggcgtagctc tggaacacca tcgagatgtc gcgccgggat gggtcgacgt
     4141 cgttgacgac ctcgtcgccg atggagagct tgccgtcgga gatgtcctcg agcccggcga
     4201 tcatgcgcag cgccgtggtc ttgccgcagc ccgaggggcc gagcagcacc atgaactcgc
     4261 cgtcctcgat ggcgaggttc aagtcgtcga ccgcggtgac ctcgccgtag cgcttcgaga
     4321 cggcgtcgaa ggtgacgctg gccatgcaat cctcctggat caggacctcg ggatgggcga
     4381 aacctacggc ccttgagcga accggaggtg acatgtcggt gaccggacgg tgacggcagg
     4441 ccaacgctcc gcgtcaccct agaggttcgt tcggcggcgg atgaccgtcc ctcggcgcgc
     4501 cggtccgctt cagtagattc gacggcgaac ccatcgctca cgacacggtc ttccatggtc
     4561 tcctccgctc tcgccgatcg cagctaccga ccctcctccg acggcttctc gatcgaaccc
     4621 gtcgccgccc agctctacgc ggcgttcgcg tccgtcgccc cgtcgtcgcc ggtcgtgcac
     4681 tgcccccact gcgtgagcac cggtgacctg gccgcgctgg cggtgccgcc ggccgcggtg
     4741 ccgccggaga ccgtgtcgcg cttcgtgcgc aagacc
//
LOCUS       DATMFS010000554         3380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_135194, whole genome shotgun sequence.
ACCESSION   DATMFS010000554 DATMFS010000000
VERSION     DATMFS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135194"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /locus_tag="VNQ33_10490"
     CDS             <1..1881
                     /locus_tag="VNQ33_10490"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWJ98577.1"
                     /translation="LDPEVLHSLEQELQRLVEGRLARDADEVTDLLRILGPLTTPELV
                     LRCAPGLAGGLDEALGRLAEDRRIISVAVAGEDRWAAAEDAGLLRDALGCAIPVGLPG
                     VFTDPVDAPLEGLVVRYGRTHGPFLDRDVAARLGIDAGRIRPLLDALVADGRLVRGEF
                     RPGGAEREWCDPEALRQLRRRSLAVLRAEVEPVDGAALGRFLPAWQGVGLPRKGIDGL
                     VEVLGVLAGASVPASVLESDVLPSRMAEYRPSDLDALCTAGDLVWLGASPLGSTDGRV
                     RLVFRDQVELLVPEPTAGERPEGLVHDALRAHLAQRGASFWAELVGAAAAAETDYDDA
                     TVLAALWDLVWAGEVTNDSLAPLRAFVSASGGRRSSSGPAPRGRPRPGRLSRLGPPTG
                     AGRWSLVSSLRGPLPGQAPPSATEIAHARAMQLLERYGVLTREAALGEGIVGGFAGVY
                     PVLKALEERGAIRRGYFVAGLGAAQFAVPGAVDRLRSAREPRRADALGGEDGRGGGDT
                     ADVAVLAATDPAQPYGASLPWPPTDGRPARAAGALVVLVAGEAAAYVEKGGRSLLAFP
                     AAAADDRWPEALAAIVRRGQRRSLEIAKIDGAPARESAHADSLRSAGFADGYKGLVLR
                     NR"
     gene            2040..2561
                     /locus_tag="VNQ33_10495"
     CDS             2040..2561
                     /locus_tag="VNQ33_10495"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF020622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2"
                     /protein_id="HWJ98578.1"
                     /translation="MPARPSSSVRALLIVVVSLLTVSAASCTPKPTLSELDHKLYAAP
                     LSSFDRWWRDEQAAANRVSRATIDRWLAASPSRLDSAAARSRPWDVSTDLCSFAPDSG
                     PSFDFRIPCIRHDFAWRNLKRLGRSTRARRLAASEQFLRDMQSTCAHRSLVSRTACRS
                     MAATYFQAVSVVS"
     gene            2586..2783
                     /locus_tag="VNQ33_10500"
     CDS             2586..2783
                     /locus_tag="VNQ33_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98579.1"
                     /translation="MTATEWYWCLAHERAEEASVRDDPDNALGPYPSAAAAADWKRLN
                     EERALKWKIEDEAWTGEDADS"
     gene            2784..>3380
                     /locus_tag="VNQ33_10505"
     CDS             2784..>3380
                     /locus_tag="VNQ33_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWJ98580.1"
                     /translation="MPEGDTLHRTADRLRPALAGATLARFEAPRLRGDRPVPGVTIEG
                     VEAVGKHLLIDFDGGLTLRTHLGMAGSWHLYAQGQRWRRPRHLLRALVAVPGWEAVCF
                     SAPTVETHRRDHPGGALGTDRDPVGHLGPDLCTPDPDLDEALRRFAEACDPSTEIAVA
                     LLDQRLASGVGNVYKSEVLHRCRVDPFAPVGDVPVEVRR"
BASE COUNT          362 a         1291 c         1254 g          473 t
ORIGIN      
        1 ctcgaccccg aggtgctgca cagcctggag caggagctgc agcggctggt cgaggggcgc
       61 ctggcccgcg acgccgacga ggtcaccgac ctgctgcgca tcctcggccc gctcaccacg
      121 ccggagctgg tgctccgatg cgcaccgggc ctggccggcg gcctcgacga ggcgctcggt
      181 cggctggccg aggatcgccg catcatctcg gtggcggtcg ccggtgagga ccggtgggcg
      241 gccgccgagg atgccggcct cctgcgcgat gcgctcggct gcgccatccc cgtggggctg
      301 ccgggcgtgt tcaccgaccc ggtcgatgcc ccgctcgaag gcctggtggt gcgctacggc
      361 cggacccacg gcccgttcct cgaccgcgac gtcgccgccc gcctcggcat cgacgccggc
      421 cgcatccggc cgctgctcga cgccctggtg gccgacggtc gcttggtgcg cggcgagttc
      481 cgaccgggtg gtgccgagcg ggagtggtgc gacccggagg cgctgcgcca gctgcgccgc
      541 cggtcgctcg cggtgctgcg agcggaggtc gagccggtcg acggcgccgc gctcggtcgg
      601 ttcctgccgg cgtggcaggg cgtcggtctg ccgaggaagg gcatcgacgg actggtcgag
      661 gtcctcggcg tgctggcggg ggcatcggtg ccggcctcgg tcctggagtc cgacgtcctc
      721 ccgtcgcgga tggccgagta ccggccatct gatctcgatg ccctgtgcac ggcgggcgac
      781 ctggtgtggc tcggtgcctc gcccctcggt tccacggatg gccgggtccg cctggtgttc
      841 cgcgaccagg tggagctgct cgtgcccgag cccaccgccg gtgaacgtcc cgaaggtctg
      901 gtgcacgacg cgctgcgcgc ccacctggcc cagcgcggtg cctcgttctg ggctgagctg
      961 gtcggggcgg cggccgccgc cgagacggac tacgacgacg ccacggtcct ggccgcgctg
     1021 tgggacctgg tctgggcggg cgaggtcacg aacgactcgc tcgcaccgct gcgcgccttc
     1081 gtgtcggcgt cgggcggtcg ccgctcctcg tcgggtccgg cccctcgggg ccgtccccgc
     1141 cctggtcggc tgagccgcct cgggccgccg acgggggcgg gccggtggtc gctggtctcg
     1201 tcgctccggg gtccgctccc gggccaggcg ccgccgtcgg ccaccgagat cgcccacgcc
     1261 cgggcgatgc agctcctcga gcgctacggc gtcctcaccc gggaggccgc gctcggcgag
     1321 gggatcgtgg gcgggttcgc gggcgtgtac ccggtgctca aggcgctcga ggagcgcggt
     1381 gcgatccgcc gcggctactt cgtggccggc ctcggtgccg cccagttcgc cgtgccgggc
     1441 gcggtcgacc ggttgcgctc ggcacgcgag ccccggcgtg ccgacgcgct gggtggcgag
     1501 gacgggcgcg gcggaggcga cacggccgac gtcgcggtcc tcgccgccac cgacccggcc
     1561 cagccctacg gcgcctccct cccgtggccg cccaccgacg gccgcccggc ccgagcggcg
     1621 ggtgcgctcg tcgtgctggt cgccggcgag gccgcggcct acgtcgaaaa gggcgggcgc
     1681 agcctgctcg ccttcccggc cgcggcggcc gacgaccggt ggcccgaggc gctcgccgcg
     1741 atcgtccggc gcggccagcg ccgctcgctg gagatcgcca agatcgacgg cgccccggct
     1801 cgcgagtccg ctcacgccga ttcgctgcga agcgccgggt tcgccgacgg ctacaagggc
     1861 ctggtcctcc ggaaccgctg accggcctcg cccggatcgg ccgatgccgg ctccgggcga
     1921 accgctcgcc tgcgacccat caggcgttgc ccgcgctcct ggccggcgcg agcctgcgat
     1981 caccgctcgc ccgtggaccg ccagggcctc cccagaccat gtcgcccctg gagctccccg
     2041 tgcctgctcg tccgtcctcg tccgtccgcg ccctgctcat cgtggtcgtg agccttctca
     2101 ccgtgtcggc cgcgtcgtgc acgccgaagc cgacgctgtc tgagctcgac cacaagctgt
     2161 atgccgcacc gctcagctcg ttcgaccgct ggtggcggga cgaacaggcg gccgccaacc
     2221 gcgtgagccg ggcgacgatc gaccggtggc tcgcagcatc gccgtcgcgg ctggactccg
     2281 cggcagcccg ttcgcgcccc tgggacgtga gcaccgacct ctgctcgttc gctcccgact
     2341 ccggaccgtc gttcgacttc cggatcccgt gcatccgcca cgacttcgct tggcgcaacc
     2401 tgaagcggct cggccggtcg acccgcgctc ggcggctggc ggccagcgag cagttcctcc
     2461 gcgacatgca gagcacgtgc gcgcatcgct cgctcgtgtc gcggacggcg tgccgctcga
     2521 tggcggccac ctacttccag gcggtctcgg tcgtgtcgtg agcggccatg ggtatcgtcg
     2581 gcaccatgac cgcaaccgag tggtactggt gcctcgccca cgagcgggcg gaggaagcct
     2641 ccgttcgcga cgaccccgac aacgccctcg gtccgtaccc gtccgcggcg gcggcggcgg
     2701 actggaagcg gctcaacgag gagcgggcgc tgaagtggaa gatcgaggac gaggcgtgga
     2761 ccggcgagga cgccgactcc tagatgcctg agggcgacac cctccaccgg acggcggacc
     2821 ggctccgccc ggcgctggcc ggggcgaccc tcgcccgctt cgaggcgccg cgcctccgcg
     2881 gcgaccggcc ggtgccgggg gtgaccatcg agggcgtcga ggcggtgggc aagcacctgc
     2941 tgatcgactt cgacggcggc ctcacgttgc ggacgcacct cgggatggcc gggtcgtggc
     3001 acctctacgc gcaaggccag cggtggcgaa gaccccggca cctgctgcgg gcgctcgtcg
     3061 ccgtgcccgg ctgggaggcg gtgtgcttct ccgcacccac cgtcgagacc caccggcggg
     3121 accatccggg cggcgcgctc ggcaccgacc gggacccggt cggccacctc ggccccgacc
     3181 tgtgcacgcc ggatcccgac ctggacgagg ccctgcgccg gttcgcggag gcctgcgacc
     3241 cgtccaccga gatcgccgtc gcgctcctcg accagcggct cgcgtcgggt gtcggcaacg
     3301 tctacaagtc cgaggtgctc caccgctgcc gcgtcgaccc gttcgctccg gtcggcgacg
     3361 tgccggtcga ggtccgccgc
//
LOCUS       DATMFS010000555         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_19212, whole genome shotgun sequence.
ACCESSION   DATMFS010000555 DATMFS010000000
VERSION     DATMFS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19212"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1020
                     /locus_tag="VNQ33_10510"
     CDS             211..1020
                     /locus_tag="VNQ33_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HWJ98581.1"
                     /translation="MDVSEVRADLLAEQEALDVVVRDLTAEQFALPTPSPRWTVADQL
                     SHLTYFDRAAVIAITQPDAFPAQVHALLEAAAGGDEAVDAFEMDEWRYLPPAELIAAW
                     RGNRGALATAAETLQNDTRVAWYGPSMGSKSFLTARLMEVWAHGQDIVDTVGADRPAT
                     DRLRHIAQLGSITRKWSYLNRGMEPPEGDVSIVLTAPSGATWTFGDEGATENTVTGPA
                     EDFCLVTTQRRHLDDTDLVVTGDVARDWLIRAQAFAGPATDGPAPRTTKEP"
     gene            1020..1790
                     /locus_tag="VNQ33_10515"
     CDS             1020..1790
                     /locus_tag="VNQ33_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ98582.1"
                     /translation="MALVEQSLANGVLTVTLNDEPRRNALSADLLVELVDILDAADAD
                     DAVRAVVLTNAGTVFCAGANLSQQSSGATAGRTVDMASVFARFRTSPKPYVGRIAGHC
                     VAGGMGLAAAMDISVAVDTAKFGFTEVRVGVAPAMISVLCLPKLREADARAAFLRGNR
                     FLADEAARIGLINRAVPAEELDAAVDEIVTDLLAGGPNAIAASKRLLAEVPGMPVDDA
                     FAWTQKFSAELFTSDEAKEGMGAFLEKRPASWVPQLEA"
     gene            1855..2460
                     /locus_tag="VNQ33_10520"
     CDS             1855..2460
                     /locus_tag="VNQ33_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98583.1"
                     /translation="MTRDLAMTTTSAKSGGTHRTRLATGALAVAAVLLAVVGTGGVAE
                     SQVAPPVVTASATDGLLIESGNCSGSSTFSLDPATVSVTRTGDTTNELHVGVAWSGTI
                     GASVYGAPEGLVIPAGETTAVFEIESDHEAGTVTIEIGASPDPSAPYDIGDPSTATAR
                     VLQAIADPVCQTAVSVPTTTPPPSPQPRPAAPVTADPDYTG"
     gene            complement(2512..>2908)
                     /locus_tag="VNQ33_10525"
     CDS             complement(2512..>2908)
                     /locus_tag="VNQ33_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98584.1"
                     /translation="LGASIGTALAGSVMIAVLTSTAIAGINATTAIPQEVQKQASVEL
                     ADGIPFVSDTQLEEGLAGTDLSADAGDAIVDANRQARIDGLDTALAVLAMLAIVSLFF
                     TRRLPDAQPTGGDAAAGTPPEPAQPSRTS"
BASE COUNT          415 a         1114 c          987 g          392 t
ORIGIN      
        1 gcccaaagac gccgccgatg cggtcgtcga cctcatcctc cgcggcctgg ccgcccccgc
       61 cgccgatggg tgagcgcacc gaggccgacg tctccgcccc gactgcgctc accgttgggt
      121 ggcggggggc accgaggtgc acccatcgcc cgcacgtgcg cccacctcgg atgtggcggg
      181 tgcccatgaa ccgacggaaa ggctgacccg atggacgtat ccgaagtccg agctgacctg
      241 ctggcggagc aggaggcgct cgacgtggtc gtgcgcgacc tgacggccga gcagttcgcc
      301 ctgcccaccc ccagcccccg ctggacggtg gccgaccagc tgagccacct cacctacttc
      361 gaccgggcgg cggtcatcgc catcacccag cccgacgcgt tcccggccca ggtccacgcc
      421 ctgctcgaag cggcggcggg cggcgacgag gcggtcgacg ccttcgagat ggacgagtgg
      481 cgctacctcc cgcccgccga gctgatcgcc gcctggcggg gcaaccgcgg cgcgctcgcc
      541 accgcggcgg agacgctgca gaacgacacc cgggtcgctt ggtacgggcc gtcgatggga
      601 tcgaagtcgt tcctcaccgc ccgcctcatg gaggtgtggg cgcacggcca ggacatcgtc
      661 gacacggtgg gcgccgaccg gccggccacc gaccggctgc gccacatcgc ccagctcggg
      721 tccatcaccc ggaagtggag ctacctcaac cggggcatgg agccgccgga gggcgacgtg
      781 tccatcgtgc tcaccgcccc gtccggtgcc acgtggacct tcggcgacga aggtgccacc
      841 gagaacaccg tcaccggtcc ggccgaggac ttctgcctcg tcaccaccca gcggcgccac
      901 ctcgacgaca ccgacctggt ggtcaccggc gacgtcgccc gagactggct gatccgagcc
      961 caggccttcg ccggccccgc caccgacggc cccgcgccgc gcaccaccaa ggagccctga
     1021 tggccctcgt cgaacagtcc ctcgccaacg gcgtcctcac cgtcaccctc aacgacgagc
     1081 cccgccgcaa cgcgctcagc gccgacctgc tggtggagct ggtcgacatc ctcgacgccg
     1141 ccgatgccga cgacgccgtg cgcgccgtcg tcctcaccaa cgccggcacc gtgttctgcg
     1201 ccggcgccaa cctgtcgcag cagtcatccg gtgccactgc cggccggacg gtcgacatgg
     1261 cgagcgtgtt cgcccgtttc cgcacgtcgc ccaagcccta cgtcgggcgc atcgccggcc
     1321 actgcgtcgc cggcggcatg ggcctggccg cagcgatgga catctcggtc gcggtcgaca
     1381 cggcgaagtt cggcttcacc gaggtccgcg tcggcgtcgc ccccgccatg atctcggtcc
     1441 tctgcctgcc gaagctccgc gaggccgatg cccgcgccgc cttcttgcgc ggcaaccgct
     1501 tccttgccga tgaggccgcc cgcatcgggc tcatcaaccg ggcggtcccc gcagaggagc
     1561 tcgacgccgc ggtcgacgag atcgtgaccg acctcctggc cggcggcccc aacgccatcg
     1621 ccgccagcaa gcgcctcctc gccgaggtcc ccggcatgcc cgtcgacgat gccttcgcct
     1681 ggacccagaa gttcagcgcc gagctgttca ccagcgatga ggccaaggag ggcatgggcg
     1741 ccttcctcga gaagcgccct gccagctggg tcccgcagct tgaggcctga gcctccgcct
     1801 ggtccgaccg gccctcggac ggaagtccta gggttcggct cgtaggaccc gacgatgacg
     1861 agggatctgg cgatgacgac gacgagtgca aagagcggtg gcacgcaccg gacccggctg
     1921 gcgaccggag cgttggccgt ggccgcggtc ctgctcgccg tcgtcggcac gggcggcgtc
     1981 gccgagagcc aggtcgcccc gccggtggtc accgcgtcgg ccaccgatgg cctcctcatc
     2041 gaatccggca actgcagcgg gtccagcacc ttctcgctcg acccggccac cgtctcggtc
     2101 acgcgcaccg gcgacaccac gaacgaactg cacgtcggcg tggcctggag cggcaccatc
     2161 ggcgcgtcgg tgtacggcgc gcccgagggt ctggtgatcc ccgccggcga gacgacggcg
     2221 gtgttcgaga tcgagtcgga ccacgaagcg ggcacggtca cgatcgagat cggggcctcg
     2281 cccgacccgt cggctcccta cgacatcggt gaccccagca cggccaccgc ccgggtcctc
     2341 caggccatcg ccgacccggt gtgccagacc gcggtgtccg tgcccaccac cacgccgccg
     2401 ccctcgcccc agccgcgacc cgccgctccc gtcaccgctg atcccgacta caccggctga
     2461 ccccctgcga tcgaacttcg tagctctggg cgaccgtccg ggcgctctcg cctacgaagt
     2521 ccgggatggt tgcgccggct ccggcggggt tccggcggcg gcgtcgccac cggtcggttg
     2581 ggcgtcgggg aggcggcggg tgaagaacag gctcacgatg gcgagcatgg ccaagacggc
     2641 cagggcggtg tcgaggccgt cgatgcgggc ctggcggttg gcgtcgacga tggcatcgcc
     2701 cgcgtcggcc gacaggtccg tgccggcgag cccttcttcg agctgggtgt cggagacgaa
     2761 cgggatgccg tcggccagct ccaccgacgc ctgcttctga acctcctgcg ggatggcggt
     2821 cgtggcgttg atgccggcga tcgcggtgga ggtgagcacg gcgatcatca ccgagccggc
     2881 gagcgcggtg ccgatcgatg cgccgagg
//
LOCUS       DATMFS010000556         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_322341, whole genome shotgun sequence.
ACCESSION   DATMFS010000556 DATMFS010000000
VERSION     DATMFS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322341"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VNQ33_10530"
     CDS             complement(<1..1272)
                     /locus_tag="VNQ33_10530"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWJ98585.1"
                     /translation="MIGPGLRGALHVSGCTSDAGKSTLVAGLCRLLARNGVSVAPFKA
                     QNMALNSYVTRTGDEIGRAQGAQALAAGLEPTVAMNPILLKPTGERTSQVVVMGQPVG
                     VMTAAEYHGHKPKLRKLVLDALAGLRDQFDVVLCEGAGSPAEINLLPHDLVNLSIARD
                     GGMRSIVVGDIDRGGVFAHLYGTVALLPDDLRATVGGFVINRFRGDPVLLGDGMDQLE
                     RACGVPTLGVLPMLSGMWLDGEDSVALGVPIPSAGTALADPLDLVAIRLPQLSNYTDL
                     DALALEPGVSVRYVADAASIGRPDLVVVPGSKETVSDLAWLRAQGIDAAIEASGADVL
                     GICAGYQLLGDTITDEVESSAGTVPGLGWLPGVTTTFGPDKVLRQHDGERASGYQIHH
                     GRVSGPEGAGWVPLDGGDEGTIRPDARVRGTT"
     gene            complement(1269..>2730)
                     /locus_tag="VNQ33_10535"
     CDS             complement(1269..>2730)
                     /locus_tag="VNQ33_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ98586.1"
                     /translation="LLDVAVSGINRVERDGVAHEHPARFVLIGSMNPEEGELRPQLLD
                     RFGLVVDVSASADRGERAEAVRRRLTADRGDAALDAHRVEDAAIAARLASIGRPPVPP
                     DAVIDLASAIALEVGAEGLRADLMLCRAAAASAAWRGAGGPDQDDLRTVAPLVLGHRR
                     RRSPLDPPGIPPEELDQAFDQAGQPGEPEPGGAPGSSDGQDQEGGLDAPAKPPAEPLA
                     PPVLHAPTARRDARAGRRGSGEADRGRFVRAAEPAPDRPVTAGTVAVAATAIATATRR
                     AGEPVGADADAGATIEASDLRRAVREQRTGALVILCVDASGSMGADRRIELAKGAVLG
                     LLTDAYQRRDRVALVTFGGAGAHVALRPTASVEIAKARLAELPTGGTSPIAEGLDAVH
                     QLVVGSSGDHGLDPLVVVITDGRATGSDDAVERSRASAGRLAAAGVATVVIDAESGAT
                     RLGLAAELADAAGGPCLPLDGLVDGSLERTIRLQLR"
BASE COUNT          337 a         1002 c         1060 g          331 t
ORIGIN      
        1 ggtcgtcccg cgcacccgag catccgggcg gatggtgccc tcgtcgccgc cgtcgagcgg
       61 cacccagccg gcgccctcgg ggcccgacac ccggccgtgg tggatctggt agccgctggc
      121 ccgctcgccg tcgtgctggc ggaggacctt gtcggggccg aacgtcgtcg tcacgccggg
      181 cagccagccg agaccgggga ccgtgcccgc cgacgactcg acctcgtcgg tgatcgtgtc
      241 gcccagcagc tggtagccgg cgcagatgcc gaggacgtcg gcaccgctcg cctcgatggc
      301 ggcgtcgatg ccctgggctc ggagccaggc caggtcgctg accgtctcct tcgagccggg
      361 gaccaccacc aggtcgggcc gaccgatcga cgcggcgtcg gccacgtacc ggaccgacac
      421 gccgggctcc agggcgaggg cgtcgaggtc ggtgtagttg gagagctgcg gcaaccggat
      481 cgccaccaag tcgagcggat cggcgagggc cgtgccggct gacgggatgg ggacgccgag
      541 ggcgacggag tcctcgccgt cgagccacat gcccgacagc atcgggagca cgccgagggt
      601 cggcaccccg cacgctcgct ccaactggtc catgccgtca cccagcagga ccgggtcgcc
      661 ccggaaccgg ttgatgacga acccgccgac cgtggcccgg aggtcgtcgg gcaggagggc
      721 gaccgtgccg tagaggtggg cgaagacccc gccccggtcg atgtcgccga cgacgatcga
      781 ccgcatgccg ccgtcacggg cgatgctcag gttgacgagg tcgtggggca gcaggttgat
      841 ctcggccggc gagcccgctc cttcgcagag cacgacgtcg aactggtcgc ggagcccggc
      901 gagcgcgtcg agcaccagct tccgcagctt cggcttgtgc ccgtggtact ccgccgccgt
      961 catcacgccg accggctgac ccatcaccac gacctgcgag gtccgctcac cggtcggctt
     1021 cagcaggatc gggttcatgg ccaccgtcgg ctcgaggccg gccgccagcg cctgggcgcc
     1081 ttgggcccgg ccgatctcgt cgccggtccg ggtgacgtac gagttgagcg ccatgttctg
     1141 ggccttgaac ggggcgaccg agaccccgtt gcgggcgagc agccggcaca gaccggccac
     1201 gagggtggac ttgccggcgt cgctcgtgca gccgctcacg tgcagggcgc cgcgcaggcc
     1261 ggggccgatc accgcagctg cagccggatc gtgcgctcga gcgagccgtc gaccagtccg
     1321 tcgagcggca ggcacgggcc gcccgccgcg tcggccagct cggcggccag gcccaggcgg
     1381 gtcgcgcccg actcggcgtc gatcaccacg gtggcgaccc ccgcggcggc aagccgcccc
     1441 gcgctggcgc ggctgcgctc gaccgcatcg tccgatccgg tggcgcggcc gtcggtgatg
     1501 acgacgacca gcgggtcgag gccgtggtcg ccggagctgc cgaccaccag ctggtgcacg
     1561 gcgtcgaggc cctcggcgat gggcgacgtg ccgcccgtcg gcagctcggc gaggcgggcc
     1621 ttggcgatct cgaccgacgc cgtcgggcgg agcgcgacgt gagccccggc gccgccgaag
     1681 gtgaccaggg cgacgcggtc ccgccgctgg taggcgtcgg tcagcagccc gagcacggcg
     1741 cccttggcca gctcgatgcg gcggtcggca cccatcgagc cggaggcgtc gacgcacagg
     1801 atcacgaggg cgccggtccg ctgctcccgc acggcccgcc gcaggtcgga cgcctcgatc
     1861 gtggcgccgg cgtcggcgtc ggcaccgacc ggttcgccgg ctcggcgggt ggcggtggcg
     1921 atcgccgtgg cggcgacggc caccgtgccg gcggtgacgg gccgatccgg ggcgggctcg
     1981 gcggcgcgga cgaagcggcc gcgatcggcc tcgccgctcc cccgtcgccc ggcacgagcg
     2041 tctcggcgtg cggtgggagc gtggagcacc ggtggggcga gcggttccgc gggcggcttc
     2101 gccggtgcgt cgaggccgcc ctcttggtcc tggccgtcgg acgagcccgg tgctccgccc
     2161 ggctcgggct cgccgggctg gccggcctgg tcgaacgcct ggtccagctc ctcgggcggg
     2221 atgcccggcg ggtccagggg cgagcgcctg cggcggtggc cgagcaccag cggggcgacg
     2281 gtgcgcagat cgtcctgatc cggcccgccg gcaccgcgcc aggccgcact cgccgccgcg
     2341 gcgcggcaca acatgaggtc ggcacggagc ccctcggccc cgacctccag ggcgatggcc
     2401 gatgccaggt cgatcacggc gtcgggcggc accggcggcc ggccgatcga tgccagccgg
     2461 gcggcgatgg cggcatcttc gactcgatgg gcgtcgaggg cggcgtcgcc gcgatcggcg
     2521 gtgaggcggc ggcggacggc ctcggcccgt tcgccccggt ccgccgaggc ggacacgtcg
     2581 acgaccaagc cgaagcggtc gagcagctgc ggccgcagct cgccctcctc ggggttcatg
     2641 gagccgatga gcacgaaccg ggccggatgc tcgtgggcga ccccgtcgcg ctcgacgcgg
     2701 ttgatgccgc tcacggcgac gtcgagcagc
//
LOCUS       DATMFS010000557         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_345208, whole genome shotgun sequence.
ACCESSION   DATMFS010000557 DATMFS010000000
VERSION     DATMFS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345208"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VNQ33_10540"
     CDS             <1..581
                     /locus_tag="VNQ33_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HWJ98587.1"
                     /translation="PAWPQQAAWGPPPPPTWAPQEKRKRFGWGMVIGAFLIGGFISAI
                     VTVIALVALAFAIGTPDAIAGTHPAIAEGARSVKAGDCLDDRPSLAVVVDQTDVVDCN
                     EAHDAEVAAVIDVPGGESKPGEDDLQQYMDDACALAFGGYVGSDPDTSQYGYAAVVPS
                     AEAWKSGDHTAFCLVDTVNTPGGSGSVAGSGD"
     gene            637..1323
                     /locus_tag="VNQ33_10545"
     CDS             637..1323
                     /locus_tag="VNQ33_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ98588.1"
                     /translation="MSEPVHLEPLAQTERSTVRRLAGRGSFDRALAHSIIDEAYLAHV
                     GFVVDGAPKVLPMTYGRDGDVIYLHGAVGNAMLRASAGAEVCVTFTLLDGLVLARSAF
                     HHSMNYRSVVLLGVAERVEDTEEKRRAFDVIVDHVLPGRTVAARAASPSETRTTLVLK
                     LAIDEGSAKVRTGGPVDDEEDHGLPVWAGVIPLRLMPGEPDQDALQLEVPDLAALTPP
                     VPARPSSAPR"
     gene            complement(1280..>2742)
                     /locus_tag="VNQ33_10550"
     CDS             complement(1280..>2742)
                     /locus_tag="VNQ33_10550"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWJ98589.1"
                     /translation="STTAPNAPNATATTAAKRTAADGPVNPADLPSQDQIHGWIQEVF
                     DQGVRRPGYQADRWAEDWVQEQFEAAGLEAVRREPITVKRWEPTSWSLTATPGGGETR
                     EITGFPLPFSPPIASTELTLRAYDGTGPTPRDLPWAALVDAPLIAIPADLLATGGSAP
                     EDSAGRIVDPDGTLTGHTHVVPFAEGFGAVMQPAADGGATAFIGSLVDYPGDSCRYYV
                     PYDGEEGPIPGLWISGTDGAWLHEQLASGPVHVTIEVASTLAEHESFNILGELPGADD
                     QTVMIGSHHDGPWSSAVEDASGISLVLAQANYWARQPEEDRPHRLLFILQGGHMSGGA
                     GLHQYIEAHRDELADVVLEVHLEHAALEFTEEDGKVVPTGEPVPRWWFTSRIPDLEAA
                     VFDALTTEQVNRSMLLAPDAFGTQPPTDGAFYHSEGVPIVQFLEAPFYLFDEMDTMDK
                     IDREHLVPITRAVIRIIDSTKGVSAEQMRDGLVPEA"
BASE COUNT          362 a          958 c         1021 g          401 t
ORIGIN      
        1 agccggcctg gccccagcag gctgcctggg ggccgccgcc gccgccgacg tgggcacccc
       61 aggagaagcg caagcggttc gggtggggca tggtcatcgg cgcgttcctg atcggcgggt
      121 tcatcagcgc gatcgtcacc gtcatcgccc tcgtggccct ggccttcgcg atcggcacgc
      181 ccgacgccat cgccggcacg caccccgcca tcgccgaagg ggcccggagc gtgaaggcgg
      241 gtgactgcct cgacgaccgg cccagcctcg ccgtggtggt ggaccagacc gatgtcgtcg
      301 actgcaacga ggcccacgat gccgaggtgg cggcggtgat cgatgtgccc ggcggcgaga
      361 gcaagcccgg cgaggacgac ctccagcagt acatggacga tgcgtgcgcg ctggcgttcg
      421 gcggctacgt cgggtcggat ccggacacct cgcagtacgg ctacgccgcg gtggtcccca
      481 gcgccgaagc gtggaagtcg ggcgaccaca ccgccttctg cctcgtcgac accgtcaaca
      541 cgcccggtgg ctccggctcc gtcgcgggtt ccggggactg accgccggcc ggaattcgcc
      601 gctgcccacc cgtgggcgac tggcagcatc gtcgtcatga gcgaaccggt gcacctcgaa
      661 cccctggccc agaccgagcg cagcacggtg cgccgactcg ccggccgcgg ctcgttcgac
      721 cgggcgctcg cccactcgat catcgacgag gcgtacctcg cccacgtcgg cttcgtggtc
      781 gacggcgcgc ccaaggtgct gccgatgacc tacggccgcg acggcgacgt gatctacctg
      841 cacggagcgg tgggcaacgc catgctgcgg gcgtcggcgg gggccgaggt gtgcgtgacc
      901 ttcaccctgc tcgacgggct cgtcctcgcc cgctcggcgt tccaccactc gatgaactac
      961 cggtcggtcg tgctcctggg cgtggcggag agggtggagg acaccgagga gaagcgccgg
     1021 gcgttcgacg tgatcgtcga ccacgtgctg ccgggccgca ccgtggcggc ccgggccgcc
     1081 agcccatcgg agacgcggac cacgctcgtg ctcaagttgg cgatcgacga gggctcggcg
     1141 aaggtccgca ccggtggtcc cgtcgacgac gaggaggacc acggcctccc ggtgtgggcg
     1201 ggcgtgatcc ccctgcggct catgcccggc gagcccgacc aggatgcgct ccagctcgag
     1261 gtccccgacc tggcggcgct cacgcctccg gtaccagccc gtccctcatc tgctccgcgc
     1321 tgacgccctt ggtgctgtcg atgatccgga tcacggcccg ggtgatcggc acgaggtgct
     1381 cgcggtcgat cttgtccatc gtgtccatct cgtcgaacag gtagaagggc gcctccagga
     1441 actggacgat cggcaccccc tccgagtggt agaacgcgcc gtcggtgggc ggctgggtgc
     1501 cgaacgcgtc gggggccagg agcatcgacc ggttgacctg ctcggtggtg agggcgtcga
     1561 acacggcggc ctccaggtcc gggatgcggc tcgtgaacca ccagcggggg acgggctcgc
     1621 cggtgggaac gaccttgccg tcctcctcgg tgaactcgag ggcggcgtgc tccaggtgga
     1681 cctccaagac gacgtcggcc agctcgtcgc ggtgggcctc gatgtactgg tggagcccgg
     1741 cgccgccgga catgtggccg ccctggagga tgaagagcag ccggtgcggc cggtcctcct
     1801 cgggctgtct cgcccagtag ttggcctggg ccagcaccag cgagatgccg ctggcgtcct
     1861 cgaccgccga ggaccagggc ccgtcgtggt gcgacccgat catcacggtc tggtcgtcgg
     1921 cgcccgggag ctcgcccaag atgttgaacg actcgtgctc ggccagcgtg gaggcgacct
     1981 cgatggtgac gtgcaccgga ccggaggcga gctgctcgtg gagccaggcg ccgtcggtgc
     2041 cgctgatcca caggccgggg atcggcccct cctcgccgtc gtagggcacg tagtagcggc
     2101 acgagtcgcc ggggtagtcc acgagcgacc cgatgaacgc ggtggctccg ccgtcggccg
     2161 ccggctgcat cacggcaccg aagccctcgg cgaacggcac cacgtgggtg tggccggtga
     2221 gcgttccgtc gggatcgacg atgcgcccgg cgctgtcttc cggcgcgctg cccccggtgg
     2281 ccaggaggtc ggcggggatg gcgatgagcg gggcgtcgac gagggcggcc cacgggaggt
     2341 cccgcggcgt cgggccggtc ccgtcgtagg ccctgagcgt gagctcggtg gaggcgatcg
     2401 gcggggagaa cgggagcggg aagccggtga tctcgcgggt ctcgccgccc ccgggcgtgg
     2461 cggtgagcga ccacgacgtc ggctcccagc gcttcacggt gatcggctcg cgccgcaccg
     2521 cctcgagccc ggcggcctcg aactgctcct ggacccagtc ctcggcccag cggtcggcct
     2581 ggtagccggg tcggcggacg ccctggtcga acacctcctg gatccacccg tggatctggt
     2641 cctgggacgg gaggtctgcg gggttcaccg gtccgtcagc cgcggtgcgc ttcgcggccg
     2701 tcgtcgccgt cgcgttcggc gcgttcggtg cggtcgtcga cg
//
LOCUS       DATMFS010000558         5399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_43111, whole genome shotgun sequence.
ACCESSION   DATMFS010000558 DATMFS010000000
VERSION     DATMFS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43111"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VNQ33_10555"
     CDS             <1..261
                     /locus_tag="VNQ33_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98590.1"
                     /translation="NLACRYAHHLVAMRDGAIVAEGAPSEIVTQALVQEVFDLDARIV
                     TDPVSGTPLVVPIGRHHSSLLEAQQDEPPLPDLNHPLTEELS"
     gene            258..1187
                     /locus_tag="VNQ33_10560"
     CDS             258..1187
                     /locus_tag="VNQ33_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98591.1"
                     /translation="MKRTVSSLALVGVLLVGVAACGSSSDASGSPSDTTTAAAGGSGA
                     DDGRDLGNVLALGEEFLLADLLSLGVKPVASTATSSDAGWQGLDGFDTEGVEILPNTE
                     ANVEHLASLDIDTVIVQQYVIDQVGREKLEALGEVIVAPTGTGDEELTELGKLVGRED
                     RAAELVDQLDTATAAAEDAAGDGCEVSLATIYPGPTPAAWVAAPNNAAIALEDMGCTL
                     VPSVEDGKADGAGRIYLSLENLGELSAPQMILLQSSTVPGEDASLDQVEGESLWKQLP
                     AVEADQVTTLDRLGYPGVAGLIRLYDELGKVVG"
     gene            1187..2338
                     /locus_tag="VNQ33_10565"
     CDS             1187..2338
                     /locus_tag="VNQ33_10565"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HWJ98592.1"
                     /translation="MGSASESPAPDSPEMAAALAAQQMMLLETLNGLYDDATLLVGRI
                     LCDRRGATEVTALSVTLDGLELRLVDDEGEHHGLVPLPGTLNHIDELFLLFFAMVIDA
                     RERSGEEGTTSVEQVIATARGYRTFITSVAAVEEVNPHLRKVTFRGGDLADGFAPVGP
                     DTFLYLLLPPPGRTELTIGADFSWESVRDMAPEDQPVGAYYTLRAWRPETHELDVLMV
                     LHGDTGPASAWAARAAVGDPVALWGPRTSWSPPADADSYLLVADETGLPAVAVILETL
                     GSDAPIQVVAEVDDETVRQALPTGEHITVTWLHRDGAEAGTTTALADAVRSLPPPTGT
                     PYVWGGAESRAVTQVRRHVRGTWGLPKSQVSLVGYWRHKAHESDVDTEG"
     gene            complement(2436..3908)
                     /locus_tag="VNQ33_10570"
     CDS             complement(2436..3908)
                     /locus_tag="VNQ33_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWJ98593.1"
                     /translation="MARVLDRFHAAAADLRTVGLVPVDADDADAVIRSLEVGTRIARA
                     AQTDLVNEIEKRGLHHVGGHRSAKVMIRHTAHLSDAEALRRAKGARVMRDLPAVASAF
                     RAGRIGCCQVDRIARTHANPRIRHLLVEVEGHVAVLAARLPYREFDRHLTNGERLADE
                     DGAGDDAERHERNRDVRIGTNLDGSIWIDGNLGSLDGAVLRDVHEHFIDAELAADWGE
                     ARERLGDVATYDDLQRTEAQRRADALMAIVRAAASHQANQTGGSVIVTNVVIDLATFE
                     RHLRRVCSDEPVTDPDPRVATMFDDVIADLDAPPDEDPDPASVGAPAGEPADVEPTES
                     SEPTKGVEPVAEREPKAPRSPKDGAGFRCDTLDGTPIDPAAATANALVNHVRRVVVGA
                     DGVVVDVGRKGRLFTGARRLAVLIGETTCSWPGCNVPVTRCQADHLDGFHSRKRGPTN
                     PRNGGPACGFHNRLKEHGFTVHRDERGHWHLYRPDGTEVT"
     gene            4092..4982
                     /locus_tag="VNQ33_10575"
     CDS             4092..4982
                     /locus_tag="VNQ33_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98594.1"
                     /translation="MAEVDISSIDEVIDLYRIDLGEFVVARAALAKELRADGRKEEAK
                     AVVKLRKPTVPAWLLDQVAAEQPELIEAALAAGEALATATDETLEGDASNLRGATEAE
                     REASAAVMDAANAHLTLTADHRERIAASLRAAIADDDIRAQLRAGLLAADHEPPAMGF
                     AAEAAQTDGTKAADNKAPAAKGAAKAKLAEPPVEQTRRSKRKIRRVGTPSSAKRQPVD
                     EVDAKRKAKEAERLQAEERKRLQAALDAEANKARANADRLEEKARKAEAVAAAARAEA
                     DDATTEAVAAEAAAEAGPPD"
     gene            complement(5001..>5399)
                     /locus_tag="VNQ33_10580"
     CDS             complement(5001..>5399)
                     /locus_tag="VNQ33_10580"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HWJ98595.1"
                     /translation="MGHRDVDVSSAEPGDGSLAITIVRQVDIDVPGVAKKFINPSNTV
                     TSKDRWERAADGSGRGVSDVDIKGVPVQSTGRASLVDDGAGGCDYTIELAMKVKVPMI
                     GEKVAGALRPQLEAQIASEFAASEAWLAGH"
BASE COUNT          766 a         2030 c         1867 g          736 t
ORIGIN      
        1 aacctggcct gccgctacgc ccaccacctg gtggccatgc gcgacggcgc catcgtcgcc
       61 gagggcgccc cgtccgagat cgtgacgcag gcgctggtgc aggaggtctt cgacctcgac
      121 gcccgcatcg tcaccgaccc cgtgtcgggt acgccgctcg tggtccccat cggccgccac
      181 cacagctcgc tgctcgaagc gcagcaggac gagccgccgc tgccggatct caaccacccc
      241 ctcaccgagg agctctcgtg aagcgaaccg tctcgtccct cgccctcgtg ggcgtgctgc
      301 tggtcggcgt cgccgcctgt ggcagcagca gcgacgcctc gggctcgccg tcagacacca
      361 cgaccgcagc agccggtggc agcggggccg acgacggcag ggacctgggc aacgtgctcg
      421 ccctgggcga ggagttcctc ctggccgacc tgctgtcgct cggtgtcaaa ccggtcgcgt
      481 ccacggccac ctcgtccgac gccggctggc agggcctcga cggcttcgac accgagggcg
      541 tcgagatcct ccccaacacc gaggccaacg tcgagcacct cgccagcctc gacatcgaca
      601 cggtcatcgt ccagcagtac gtgatcgacc aggtcggcag ggagaagctg gaggccctcg
      661 gcgaggtgat cgtcgccccg accggcaccg gcgacgagga gctgaccgag ctcgggaagc
      721 tcgtcggccg cgaggaccgg gccgccgagc tggtcgatca gctcgacacg gccaccgccg
      781 ccgccgagga cgccgccggc gacggctgcg aggtctccct cgccaccatc taccccggtc
      841 ccaccccggc cgcctgggtc gccgcaccga acaacgcggc catcgccctc gaggacatgg
      901 gctgcacgct cgtcccgtcg gtcgaggacg gcaaggccga cggcgccggt cggatctacc
      961 tgtcgctcga gaacctgggt gagctgtcgg ccccgcagat gatcctcctg cagtcgtcga
     1021 ccgtcccggg cgaggacgcc tcgctcgacc aagtcgaagg cgagtccctc tggaagcagc
     1081 tgccggcggt ggaggccgat caggtgacca cgctcgaccg gctcggctac ccgggcgtcg
     1141 ccggcctgat ccgcctgtac gacgagctcg ggaaggtggt cggctgatgg gcagcgcatc
     1201 ggagtccccc gcgcccgact ccccggagat ggccgccgcc ctcgctgccc agcagatgat
     1261 gctgctcgag acgctgaacg gcctctacga cgacgccacc ctcctcgtcg gccgcatcct
     1321 gtgcgaccgc cgcggcgcca ccgaggtgac tgccctgtcc gtcaccctcg acggcctgga
     1381 gctgcgcctc gtcgacgacg agggcgagca ccacggcctg gtcccgctgc ccgggacgct
     1441 caaccacatc gacgagctgt tcctgctgtt cttcgccatg gtcatcgatg cccgggagag
     1501 gtccggcgag gagggcacga cgtcggtcga gcaggtcatc gccaccgccc gcggctaccg
     1561 gacgttcatc acgtcggtcg ccgcggtcga ggaggtcaac ccgcacctcc gcaaggtcac
     1621 gttccgaggt ggcgacctgg cggacggctt cgcccccgtc ggccccgaca cgttcctcta
     1681 cctgctgctc ccgccgccgg ggcgcaccga gctgaccatc ggcgccgact tctcctggga
     1741 gtcggtccgg gacatggccc ccgaggacca accggtcggc gcgtactaca cgctgcgcgc
     1801 ctggcggccc gagacgcacg agctcgacgt cctcatggtc ctccacggcg acaccggccc
     1861 cgcctcggcc tgggccgccc gcgccgccgt cggcgacccc gtcgccctgt ggggtcctcg
     1921 cacctcctgg tcaccgcccg ccgatgcgga ctcgtacctg ctcgtggccg acgagaccgg
     1981 cctgcccgcc gtcgccgtca tcctcgagac gctcggcagc gacgctccca tccaggtggt
     2041 ggccgaggtc gacgacgaga ccgtccgcca agcgctcccc accggcgagc acatcaccgt
     2101 gacgtggctc caccgcgacg gcgccgaggc cggcaccacc accgccctgg ccgacgcggt
     2161 gcgctcgctc cccccgccca ccggcacgcc ctacgtgtgg ggcggcgccg agagccgagc
     2221 cgtcacccag gtccgccgcc acgtccgcgg cacctggggc ctccccaagt cccaggtctc
     2281 cctcgtcggc tactggcgtc acaaggccca cgagtccgac gtcgacaccg agggctagcc
     2341 cgggcggcac cacccccgat ccctggcccc accgctgacc gaggcccggc cgagcgcgct
     2401 ggagcgcccc ccagcctgct gggtcggggg ttcggtcagg tgacctcggt gccgtcaggc
     2461 cggtagaggt gccagtggcc gcgctcgtct cgatggacgg tgaagccgtg ttccttgagg
     2521 cggttgtgga agccgcaggc gggtcctccg ttgcgggggt tggtcggtcc ccgcttgcgg
     2581 ctgtggaagc cgtcgaggtg gtcggcctgg cagcgggtca ccgggacgtt gcagcccggc
     2641 caggagcagg tggtctcgcc gatgaggacg gccagccggc gggccccggt gaacaaccga
     2701 cccttgcggc cgacgtcgac cacgacgccg tcggccccca ccaccacccg ccgcacgtgg
     2761 ttcacgagcg cgttggccgt ggcggcagcc gggtcgatcg gcgtgccatc gagggtgtcg
     2821 caccggaacc cggccccgtc cttcggcgac cgcggcgcct tcggctcccg ttccgccacc
     2881 ggctcgaccc ccttggtggg ctcggacgac tccgtcggct cgacgtcggc cggttcacct
     2941 gctggggctc cgaccgaagc gggatcgggg tcctcatcgg gcggggcatc gaggtcggcg
     3001 atgacgtcgt cgaacatcgt cgcgacgcgg ggatcgggat cggtcaccgg ctcgtcggag
     3061 cagacccggc ggaggtggcg ctcgaaggtg gccaggtcga tcaccacgtt ggtgacgatg
     3121 acggagccgc cggtctggtt cgcctggtgc gaggccgccg ctcggacgat ggccatcagc
     3181 gcatcggcac ggcgctgcgc ctcggtccgt tgcaggtcgt cgtacgttgc gacgtcgccg
     3241 agccgctcgc gggcttcgcc ccaatcggcg gcgagctcgg catcgatgaa gtgctcgtgc
     3301 acgtcccgca ggacggcgcc gtcgagggac ccgaggttgc cgtcgatcca gatcgacccg
     3361 tcgaggttcg tcccgatccg cacgtcccgg ttgcgctcgt gccgttcggc atcgtcaccg
     3421 gctccgtctt cgtcggcgag ccgctccccg ttggtgaggt gccggtcgaa ctcccggtac
     3481 gggagccgag cggccagcac tgcgacatgg ccctcgactt cgaccagcag gtgccggatc
     3541 cgcgggttgg cgtgggtccg ggcgatgcgg tccacctggc agcagccgat gcgcccggct
     3601 cgaaacgccg acgccaccgc gggtagatct cgcatcaccc gggctccctt ggcccggcgc
     3661 agggcctcgg catcggacag gtgggccgta tggcggatca tgaccttggc gctgcggtgg
     3721 ccgccgacgt ggtggaggcc ccgcttctcg atctcgttca ccaggtcggt ctgcgccgca
     3781 cgagcgatcc gggtcccgac ctcgaggctg cggatcacgg cgtcggcatc gtcggcatcg
     3841 acggggacca gcccgacggt ccgcagatca gccgcggcgg catggaaccg gtccagtacg
     3901 cgcgccacgc tcggatctgc tgctgtcacc acccccacat catcctcagg ggatctgata
     3961 gcgaagcagt gcctgaaggc tggtttccgt actttccctg cctgtccggt tcgaaccgat
     4021 cgagcccggt ctgccgttcc gggcgcgacc agatggcaac ctgggcaggt gttcgcagcg
     4081 cgggacctct gatggccgag gtcgacatct cgagcatcga cgaggtcatc gacctctacc
     4141 ggatcgacct gggcgagttc gtcgttgctc gggccgccct ggcgaaggag ctgcgcgccg
     4201 acggccgcaa ggaggaggcc aaggccgtgg tgaagctgcg caagccgacc gtgccggcct
     4261 ggctcctcga ccaggtggca gcggagcagc ccgagctgat cgaggccgcg ctggccgccg
     4321 gcgaggcgct cgccacggcc accgacgaga ccctcgaggg cgacgcgtcc aacctccgcg
     4381 gcgcgaccga agccgagcgg gaggcatccg ccgccgtgat ggatgcggcc aacgcccacc
     4441 tcacgctcac ggccgaccat cgtgagcgga tcgcggcgtc gcttcgtgcc gccatcgccg
     4501 acgacgacat ccgggcccag ctccgggccg gcctcctcgc tgccgatcac gagccaccgg
     4561 ccatgggctt cgccgccgag gccgcccaga ccgatggcac caaggcagcc gacaacaagg
     4621 cgcccgccgc gaagggggcg gcgaaggcga agctcgctga gccgccggtc gagcagacgc
     4681 gacggtccaa gcgcaagatc cggcgggtcg ggacgccgag ctccgcgaag cgccagcccg
     4741 tcgacgaggt cgacgccaag cgcaaggcca aggaggccga gcgcctccag gccgaggaac
     4801 gcaagcgctt gcaggccgcc ctcgatgccg aggcgaacaa ggcgcgggcc aacgccgacc
     4861 gcctcgagga gaaggcccgc aaggccgagg cggtcgccgc cgccgctcgc gccgaggccg
     4921 acgacgccac gaccgaggcg gtcgccgccg aggccgcggc cgaagcaggc ccgcccgact
     4981 gaccgagggt cggcgcagcg tcagtgcccg gcgagccagg cttcgctggc ggcgaactcg
     5041 gaggcgatct gggcctcgag ctgggggcgc agcgcaccgg ccaccttctc cccgatcatc
     5101 ggcaccttca ccttcatggc cagctcgatg gtgtagtcgc acccgccggc accatcgtcg
     5161 accagggacg cccgcccggt ggactgcacc ggcacgccct tgatgtcgac gtcgctgacg
     5221 ccgcggcccg acccgtcagc cgcgcgctcc cagcggtcct tggacgtcac cgtgttggag
     5281 gggttgatga acttcttggc gacgccgggc acgtcgatgt cgacctggcg gacgatggtg
     5341 atggcgagcg agccatcgcc cggctcggcg ctcgagacgt cgacgtcgcg gtggcccat
//
LOCUS       DATMFS010000559         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_43160, whole genome shotgun sequence.
ACCESSION   DATMFS010000559 DATMFS010000000
VERSION     DATMFS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43160"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VNQ33_10585"
     CDS             complement(<1..694)
                     /locus_tag="VNQ33_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HWJ98596.1"
                     /translation="MTDTTASVSSAASGVHSEVGQLRKVLVCTPGLGHERLTPTNCDD
                     LLFDDVMWVQNARRDHFDFCDKMRERGVEVVELHDLLAETMDLPAARAWLLDRKIIPN
                     EVGLGLVAETRAFLDDLPSADLARFLIGGLATQDLPDDLRPGYVALARESTGVREYLM
                     PPLPNMLYTRDTTSWIYGGVTLNPLYWPARQDETLLMKAVYGFHPDLVGSTVWWGDPE
                     EHWALATIEGGDV"
     gene            complement(718..>2036)
                     /locus_tag="VNQ33_10590"
     CDS             complement(718..>2036)
                     /locus_tag="VNQ33_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005275 - amine transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HWJ98597.1"
                     /translation="YGALVAWLVAGLGMLMLCYVFQNLAMRKPHLDAGIFAYAKAGFG
                     DYVGFNSAFGYWASACVGNCFYWVFIMTTLGATFPSLGKGDTILAVAISSAGVWIFHL
                     LIARGVKDAAIINRIVTVAKVIPIVLFIILVAIGFDSSVFRDNLWGGELGSFSTLMDQ
                     VKATMIVTTFVFLGIEGATVYSRFATKREDVGKATLMGFLGVLSIFSLVTIVSYGVLP
                     RADLAGARQPSMGTVLESVVGGWGSTFINIGVIVSVLGAYLAWTLMSAEVMFIPAKND
                     DMPQFLRRENKAGTPIAALIMTSLLVQLFILVTLTSDDALNFMLDLCTSLSLIPYFLA
                     AAYAFKLTRSGETYDVATEGSLRRRQLAVAALASIYTLFLVYTAGGKFLLLSCIIYAP
                     GTVLYVMARRENGLLVFKPLELALCALLAVGGVAGVAGLVTGAITI"
BASE COUNT          359 a          607 c          781 g          289 t
ORIGIN      
        1 ggacgtcgcc gccctcgatg gtggcgagcg cccagtgctc ctcggggtcg ccccaccaca
       61 ccgtggaccc gaccaggtcc gggtggaacc cgtagacggc cttcatgagg agcgtctcgt
      121 cctggcgggc cggccagtag agcgggttga gggtgacgcc gccgtagatc cacgaggtgg
      181 tgtctcgggt gtagagcatg ttcggcagcg gcggcatcag gtactcccgc acgccggtgg
      241 actcccgggc gagggcgacg tagccggggc gcaggtcgtc gggcaggtcc tgggtggcga
      301 gcccgccgat gaggaagcgg gccaggtccg cgctgggcag gtcgtcgagg aacgcccggg
      361 tctcggcgac gaggccgagg ccgacctcgt tcgggatgat cttgcggtcc agcagccagg
      421 ctcgggccgc gggcaggtcc atcgtctcgg cgagcaggtc gtgcagctcg acgacctcga
      481 cgccgcgctc gcgcatcttg tcgcagaagt cgaagtggtc ccggcgggcg ttctgcaccc
      541 acatcacgtc gtcgaacagc aggtcgtcgc agttcgtggg ggtgaggcgc tcgtggccga
      601 ggcccggcgt gcacaccagg accttgcgca gctggccgac ctcggagtgg acgccggagg
      661 cggcggacga gacggaggcg gtggtgtcgg tcatcggtgg tgctccaggt ggaggggcta
      721 gatggtgatg gcgccggtga cgagaccggc cacgccggcg acgccgccga cggcgaggag
      781 ggcgcacagg gcgagctcga gcggcttgaa gacgagcagg ccgttctccc gtcgggccat
      841 gacgtacaag acggtgcccg gcgcgtagat gatgcagctg agcagcagga acttgccgcc
      901 tgccgtgtag acgaggaaca gcgtgtagat cgacgcgagc gcggccacgg cgagttgacg
      961 ccggcgcagg ctgccctcgg tggcgacgtc gtaggtctcg ccgctgcggg tgagcttgaa
     1021 ggcgtaggcg gcggcgagga agtaggggat cagcgacagg ctcgtgcaca ggtcgagcat
     1081 gaagttgagg gcgtcgtcgg aggtgagggt gacgaggatg aacagctgga cgagcagcga
     1141 ggtcatgatc agcgcggcga tgggggtgcc ggccttgttc tcccggcgga ggaactgggg
     1201 catgtcgtcg ttcttggccg ggatgaacat gacctccgcc gacatcagcg tccaggcgag
     1261 gtaggcgccg aggaccgaga cgatcacacc gatgttgatg aacgtcgagc cccacccgcc
     1321 gacgaccgac tccagcacgg tgcccatcga cggctggcgg gcaccggcga ggtcggcgcg
     1381 ggggagcacg ccgtaggaca cgatcgtgac gagcgagaag atgctgagca cgcccaagaa
     1441 gcccatcagc gtggccttgc ccacgtcttc gcgcttcgtg gcgaaccggg agtagacggt
     1501 ggcgccctcg atgccgagga agacgaacgt ggtgacgatc atcgtggcct tcacctggtc
     1561 catcagcgtc gagaacgagc cgagctcccc gccccacagg ttgtctcgga acaccgacga
     1621 gtcgaagccg atggcgacga ggatgatgaa caggacgatc gggatcacct tcgccaccgt
     1681 gacgatgcgg ttgatgatgg cggcgtcctt cacgccgcgg gcgatgagga ggtggaagat
     1741 ccacacgccg gccgacgaga tggcgacggc gaggatcgtg tcgcccttgc cgaggctggg
     1801 gaacgtggcg cccagcgtcg tcatgatgaa gacccagtag aagcagttgc cgacgcaggc
     1861 gctcgcccag tagccgaacg ccgagttgaa gcccacgtag tcgccgaacc cggccttggc
     1921 gtaggcgaag atgccggcgt cgaggtgggg tttgcgcatc gccaggttct ggaacacgta
     1981 gcagagcatc agcatcccga ggccggcgac gagccaggcg acgagggcgc cgtaga
//
LOCUS       DATMFS010000560         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_158832, whole genome shotgun sequence.
ACCESSION   DATMFS010000560 DATMFS010000000
VERSION     DATMFS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158832"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2106)
                     /locus_tag="VNQ33_10595"
     CDS             complement(<1..2106)
                     /locus_tag="VNQ33_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98598.1"
                     /translation="MRRFAVLSAVVMLGVALVSCTSDAGDGASSTSEPSGSAAALPAP
                     ETAGLIPEDEWRQQQDDYLTFATSGEMNPGSSQSLLAYATAAERAGKEADLSSATPED
                     FADTFTKLEAFKDTGDFDINNMLFLWQRHHDDLSPDLAAAIKERILAFKYWWTEPTPE
                     GVVDSQYYWTENHQIIFLANEYIAGQTFPDEVFTNSGMTGKEHMAHAEEELAKWFEWR
                     SRFGFSEWLSNVYWSEDMKGTLLLAEFADDAKLARLGAMTLDMMFVELAGHVQEGTFG
                     STHGRTYQKDKLNGRDEDTFSTAKLVFDNTPVGYEHADNATLLATAERYRPPEVAKKI
                     ANSDQTAVFRTKSSLPIDPNAPVDPDVEAPYGTTFKGQDGLMLWWGLGAQFPWQVVPT
                     SVQAVKDYDLFETSNFQQAKDLEPVLESTDDAGLQQLALSLGVQVNPGLLSQVDTYTW
                     RSPHVMLSTAQDWRPGQRGEQDHIWQATFDPDTLVFTQHPRVPVSSADDASDNTSHWT
                     GDGGIPRSAQVENVNISLYDPAYEGDGGVGSGSYSFTYEDYTHAWFPTEHFDQVVQRD
                     GWTFGRKGDGYIALWSARPTEWRKYKEGEFTRDLTEDFDLMAPGGPNNVWISEVADAS
                     DYDSFDDFVTAITATAPVATGPFSCDDDSCADDPITVTYDSPSQGTLTYGWDKSKPLG
                     DQAPFTQDGKDVDLHPDGLR"
     gene            2200..>2636
                     /locus_tag="VNQ33_10600"
     CDS             2200..>2636
                     /locus_tag="VNQ33_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ98599.1"
                     /translation="MIEVPGGHRADPGAPPFPGGVAVSHLRVYESTSVDGLAGGTPHC
                     HTACTEAYAVIGGRGKVVTVGGDGYQETPLEAGSFAWFTPGTIHRLVNDDHLEILVLM
                     ANAGLPEAGDMVITFPDDVLADPVRYREAAALPADAATTAADDA"
BASE COUNT          356 a          869 c          954 g          457 t
ORIGIN      
        1 gcgcaggccg tcggggtgga ggtcgacgtc cttgccgtct tgggtgaacg gcgcctggtc
       61 gcccagcggc ttcgacttgt cccagccgta ggtcagcgtt ccctggctcg gtgagtcgta
      121 ggtgaccgtg atcgggtcgt cggcgcacga gtcgtcgtcg cagctgaacg ggcccgtggc
      181 cacgggcgcc gtcgccgtga tggccgtcac gaagtcgtcg aacgagtcgt agtcggatgc
      241 gtcggccacc tcggagatcc agacgttgtt cgggcctccc ggagccatga ggtcgaagtc
      301 ctcggtgagg tcccgggtga actcaccctc cttgtacttc cgccactcgg tcgggcgggc
      361 cgaccagagg gcgatgtagc cgtcgccctt gcggccgaag gtccagccgt cgcgctgcac
      421 gacctggtcg aagtgctcgg tggggaacca ggcgtgcgtg tagtcctcgt aggtgaacga
      481 gtacgagccg ctgcccacgc cgccgtcgcc ctcgtaggcc gggtcgtaga ggctgatgtt
      541 cacgttctcc acctgagcgg agcgggggat gccgccgtcg ccggtccagt ggctggtgtt
      601 gtccgaggcg tcatccgcgc tcgacaccgg gacccgcggg tgctgggtga acacgagcgt
      661 gtcggggtcg aacgtggcct gccagatgtg gtcctgctcc ccgcgctggc ccggccgcca
      721 gtcctgggcg gtggagagca tcacgtgggg cgaccgccac gtgtacgtgt cgacctgcga
      781 cagcagcccg gggttcacct gcacgcccag cgacagggcc agctgctgca ggccggcgtc
      841 gtcggtggac tcgagcaccg gctcgaggtc tttggcctgc tggaagttgg aggtctcgaa
      901 caggtcgtag tccttcaccg cctggaccga ggtgggcacg acctgccacg ggaactgggc
      961 gccgaggccc caccacagca tcagcccgtc ctggcccttg aaggtggtcc cgtagggcgc
     1021 ctcgacatcg gggtcgacgg gcgcgttcgg gtcgatcggc aggctcgact tggtgcggaa
     1081 cacggccgtc tggtccgagt tggcgatctt cttggcgacc tccggcgggc ggtaccgctc
     1141 ggcggtggcg aggagcgtgg cgttgtcggc gtgctcgtag ccgacggggg tgttgtcgaa
     1201 cacgagcttg gccgtggaga acgtgtcctc gtcgcggccg ttgagcttgt ccttctggta
     1261 cgtgcggccg tgggtgctgc cgaacgtgcc ctcctgcacg tgcccggcca gctccacgaa
     1321 catcatgtcg agggtcatgg ccccgaggcg ggcgagcttg gcgtcatcgg cgaactcggc
     1381 gagcaggagg gtgcccttca tgtcctcgct ccagtagacg ttcgacagcc actcgctgaa
     1441 gccgaagcgc gaccgccact cgaaccactt ggccagctcc tcctcggcgt gggccatgtg
     1501 ctccttgccc gtcatgccgc tgttggtgaa cacctcgtcg gggaaggtct ggccggcgat
     1561 gtactcgttg gcgaggaaga tgatctggtg gttctcggtc cagtagtact ggctgtccac
     1621 gacgccctcg ggcgtgggct cggtccacca gtacttgaag gcgaggatcc gctccttgat
     1681 ggcagcggcc aggtccgggc tcaggtcgtc gtggtggcgc tgccacaaga agagcatgtt
     1741 gttgatgtcg aagtcgccgg tgtccttgaa cgcttcgagc ttggtgaagg tgtcggcgaa
     1801 gtcctcgggc gtggcgctgc tgaggtcggc ttccttgccg gcgcgctcgg ctgcggtggc
     1861 gtaggcgagg agggattggg agctgccggg gttcatctcg ccggaggtgg cgaacgtgag
     1921 gtagtcgtcc tgctgctggc gccactcgtc ttcggggatg aggccggcgg tctccggcgc
     1981 cgggagcgcg gcggcgctcc cgctcggctc gcttgtcgac gaggcgccgt ctccggcgtc
     2041 gctcgtgcac gacaccaacg ccacccccaa catcaccact gcggacagga ctgcgaaccg
     2101 gcgcacctgg gctcccctcg aacgaccggc gagaacctac cctccccgtg gaccggcggg
     2161 tctcatgggg catgctgggc cgatggagcc gagcgtccga tgatcgaggt accgggcggc
     2221 caccgggccg accccggggc cccgccgttc ccgggtggcg tggcggtcag ccacctgcgc
     2281 gtctacgaga gcacgtccgt cgacggcctc gcgggcggca cgccccactg ccacacggcg
     2341 tgcaccgagg cctacgccgt gatcggcggc cgcgggaagg tcgtcacggt cggcggcgac
     2401 ggctaccagg agacgccgtt ggaggccggg agcttcgcct ggttcacgcc ggggacgatc
     2461 caccgcctcg tgaacgacga ccacctcgag atcctcgtgc tcatggccaa cgccgggctg
     2521 cccgaggccg gcgacatggt catcaccttt cctgacgacg tcctcgccga cccggttcgc
     2581 taccgcgaag ccgccgcgct gccggccgat gcggccacca ccgccgccga tgacgc
//
LOCUS       DATMFS010000561         5046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_135591, whole genome shotgun sequence.
ACCESSION   DATMFS010000561 DATMFS010000000
VERSION     DATMFS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135591"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VNQ33_10605"
     CDS             complement(<1..948)
                     /locus_tag="VNQ33_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98600.1"
                     /translation="MSDDGPLHGPHRPDSLLEKAAEQLAEVFGVERQLDKLLYQSAQE
                     VGGLQFASIFNPIPTERLTAKPRPDAINHAQAFLRSNPLSSDDTEQALGLLEILHGPH
                     APANVDVFAAFAEREKFFERLADGQSFIFVGSHLEFQDVGFNLGYLTRAAHLHGFDRL
                     DTRTTLMIGRLLGYLEVMGLNVIDDILRKAANVLKTFPVSGGEAVNEGDITDEDLNRR
                     VRNFRKKQNARTREEFMNLMTSDHGHLVIEAGSGSRDAQDEHGYVVMEPFAGGTREDL
                     YLAVQAGAAIYPVFGDYGNTEEPSVVEIGREITQLSSPQH"
     gene            complement(1355..1816)
                     /locus_tag="VNQ33_10610"
     CDS             complement(1355..1816)
                     /locus_tag="VNQ33_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HWJ98601.1"
                     /translation="MASGERRMLRIAGADVPYPEPTTVGASNSGKGNTRTDTKPELAL
                     RSALHRSGLRFRKDLRVKLGDVAARPDIVFTKRKVAVFVDGCFWHCCPDHGTRPKSNT
                     TYWLPKLEANVARDRRNDAALEAAGWKVVRVWEHEPVDEAADRVRKALGGG"
     gene            complement(1995..2312)
                     /locus_tag="VNQ33_10615"
     CDS             complement(1995..2312)
                     /locus_tag="VNQ33_10615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWJ98602.1"
                     /translation="MLRGDVYRLKLPKAMGHEQDGPRYGIVVQADALGQRSTVLLAPT
                     SLSAWPASFRPMVEVRGQATRVLTEHIGSYDLRRLGQRVGHLDVEEIWSIDEAIRVVF
                     GLR"
     gene            complement(2299..2547)
                     /locus_tag="VNQ33_10620"
     CDS             complement(2299..2547)
                     /locus_tag="VNQ33_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWJ98603.1"
                     /translation="MLVMPRAVSVRLDEDAIRALRIIEATGLSRSEAIRAAIMGQAGR
                     LREPASLAAEVAALEANETDRAEMAIVASIMEEGRAPW"
     gene            complement(2562..2795)
                     /locus_tag="VNQ33_10625"
     CDS             complement(2562..2795)
                     /locus_tag="VNQ33_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98604.1"
                     /translation="MFRSIARIGAEMLLLVADSARSLNLVDTEDHLIGLGYLAVLDEI
                     DAVAPYPLCLQAHPVLAAAPRRRDLRLDRRDAV"
     gene            complement(2984..3847)
                     /locus_tag="VNQ33_10630"
     CDS             complement(2984..3847)
                     /locus_tag="VNQ33_10630"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98605.1"
                     /translation="MTKRAVVTGANSGIGLATAHRLARDGFEVWAGARSADGLAAIEQ
                     AAADAGLDSIRPVPLDVTDGASVHAAFAAIQADGPVDVLVNNAGITGAGSVEETPIET
                     YQAMFDTNVLGVVRCIQEVLPGMRAARSGRIVNISSSSAIQSPPLMAPYATTKMAVEG
                     ISESLQAEVAPYGIRVAAIQAGTIMTPIWGKNPPPPDDTPYPEARDFLLKVLSHQLMN
                     SGVPAEAVADVIADAVATDAPPLRHLVGDAEHLAVLQAKHGDEVIEAFFLDREAFRRR
                     YADLAGIDYWA"
     gene            complement(4355..4822)
                     /locus_tag="VNQ33_10635"
     CDS             complement(4355..4822)
                     /locus_tag="VNQ33_10635"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWJ98606.1"
                     /translation="MASLTGRAANFMSRRFPRLVAKGTYKQVEQYRSSGGTKGNAMFG
                     KPVFLLEAVGRTSGEPRPVMLMLVRRGDDLIVVGSNGGNPVTPNWYRNLMAAERTEVQ
                     VGTDRWWTAAREVEGDERDECWALAAKAYPDFDTYQELTDRRIPVAVLERVAD"
BASE COUNT          793 a         1738 c         1725 g          790 t
ORIGIN      
        1 gtgctgcgga ctcgacagct gggtgatctc ccgaccgatc tccaccaccg acggctcttc
       61 ggtgttcccg tagtcgccga agaccgggta gatcgccgcg cccgcctgca cggcgaggta
      121 gaggtcctcg cgggtgccgc cggcgaaggg ctccatgacg acgtagccgt gctcgtcttg
      181 ggcgtcgcgg gaaccggagc cggcctcgat gacgaggtgg ccgtggtccg aggtcatcag
      241 gttcatgaac tcctcgcggg tgcgggcgtt ctgcttcttg cggaagttgc ggacccggcg
      301 gttgaggtcc tcgtcggtga tgtcgccctc gttgaccgcc tcgccgccgc tgacggggaa
      361 cgtcttgagg acgttggccg ctttgcggag gatgtcgtcg atgacgttga ggcccatgac
      421 ctcgaggtag ccgaggaggc gcccgatcat gagggtcgtg cgggtatcga ggcggtcgaa
      481 gccgtggagg tgggcggccc gggtgaggta gccgaggttg aagccgacgt cctggaactc
      541 caggtgcgac cccacgaaga tgaacgactg cccgtcagcg aggcgttcga agaacttctc
      601 ccgctcggcg aacgcggcga agacgtcgac gttcgcgggc gcgtgcgggc cgtggaggat
      661 ctcgagcagc ccgagcgcct gttcggtgtc gtcgctcgac agcgggttgg atcggaggaa
      721 cgcctgggcg tggttgatgg catcgggccg gggcttggcg gtgagccgct ccgtcgggat
      781 cgggttgaag atgctggcga actggaggcc gccgacctcc tgggccgact ggtacagcag
      841 cttgtcgagc tgccgctcca ccccgaacac ctcggcgagc tgctccgcgg ccttctccag
      901 cagcgagtcc ggccgatgcg gcccgtgcag cggcccatcg tcgctcatcg cccctcctcc
      961 ttggctcgct cgacgtggca gccgccggcc cggccgcggc ggatcagggc gcatggaggg
     1021 aggagcacgc acgttggacc tgcctgaccc gcggcgggtt accccttcgc cccggcaccg
     1081 acgaagaagg cccggcgccg gttgggtctc ttcctccctc gcgaccgaga ccccgatgcc
     1141 gagctccgct atcgagacag ccgcgacggc cggcagatcg ccgtcaccga tgagggaagc
     1201 aagacccgct ggcaagaggc caccgagccg ctcgcatggt ggatggcgtc gagacgtgcc
     1261 atgggtcgga ccatacgaac gaggtctggc agcgagccgg cccgatgacc tggaaatcgg
     1321 acatcgccac gcatcaggcg cagctgatcg gcggtcaccc gccgccgagc gcctttcgga
     1381 ctcggtcggc ggcctcatcg acgggctcgt gctcccagac cctgacgacc ttccagccgg
     1441 ctgcttcgag ggcagcgtcg ttgcggcggt ccctggcgac gttggcttcg agcttcggga
     1501 gccagtaggt ggtgttcgac ttcggccggg tgccgtggtc ggggcagcag tgccagaagc
     1561 agccgtcgac gaacaccgcg accttgcgct tcgtgaagac gatgtcgggg cgggcggcga
     1621 cgtcgccgag cttgacgcgc aggtccttgc ggaaccggag gccgctgcgg tggagcgccg
     1681 agcggagggc gagctcgggc ttcgtgtcgg tgcgggtgtt gcccttgccc gagttggagg
     1741 cgccgaccgt ggtgggctcc gggtacggca cgtccgcccc ggcgatccgc agcatgcgcc
     1801 gttcccccga agccatgccc tcaggctcgc cccttccccg cccgccggca accacgtccg
     1861 acccgcacgt ccggaacccg cttccaaagc gagaccagct catcggtcga cgtctgctgc
     1921 aagcccgcgt gaacaccgaa ccagggggag aaggccatgc gaggcgtgtt cgccctcgcc
     1981 ccggcgatgc cgccctaccg aagcccgaag acgacccgaa tcgcctcatc gatgctccag
     2041 atctcctcca cgtcgagatg tccgactcgc tgccccaacc gacgaaggtc ataggagccg
     2101 atgtgctcgg tcaaaacccg tgtcgcctgc ccccgcacct cgaccatcgg acggaacgac
     2161 gccggccagg cgctgagcga cgtgggtgcg agaagcacag tcgagcgctg gcccagggca
     2221 tccgcctgga ccacgatgcc gtatcggggt ccgtcctgct catggcccat ggccttgggg
     2281 agcttcagcc gatagacgtc accacggagc acggccttcc tccatgatcg acgccacgat
     2341 cgccatctcc gctcggtccg tctcgttggc ctccaaggcc gccacctccg cggcaagcga
     2401 cgccggctca cgaagccgac ctgcttgacc catgatcgcg gcgcggatcg cctcggatcg
     2461 gctgagaccg gtcgcctcga tgatccgcaa ggcgcggatc gcatcctcgt cgagtcggac
     2521 agaaaccgcg cgcggcatga cgagcatcgt atcgcgcatt gtcatacggc atcccgccga
     2581 tcgagccgta gatctcgccg ccgcggggcc gccgccagga cggggtgcgc ctggaggcac
     2641 agcggatacg gagccaccgc gtcgatctca tcgaggacgg cgaggtatcc gagcccgatg
     2701 aggtggtcct cggtgtccac caggttgaga gaccgagccg agtcggcgac caagagcagc
     2761 atctcagccc cgatgcgtgc gatggagcgg aacatcggcg cgacaacagc ggcatcaaac
     2821 gacttcggcg gatcgccgcg acgtgttgcg gccaggacgt cagaagcttc gagcaggtcg
     2881 ccaaccgttc tccgcacacg aggatgttgg gaacgttcat gccgtgacgg tacggccggg
     2941 gtctgacagt gaagcagacc cgaacaagtc cagctggcga gggtcaggcc cagtagtcga
     3001 tgccggccag atcggcgtag cggcggcgga aggcttcgcg gtcgaggaag aaggcctcga
     3061 tgacctcgtc gccgtgcttg gcctggagca cggcgaggtg ctcggcatca ccgacgaggt
     3121 ggcgcagcgg cggggcatcg gtggcgaccg catcggcgat gacatccgcg accgcctcgg
     3181 ccgggacgcc gctgttcatg agctggtgcg agaggacctt gaggaggaag tcccgggctt
     3241 cggggtaggg cgtgtcatcg ggcggcggcg ggttcttgcc ccagatgggg gtcatgatcg
     3301 tgccggcctg gatggcagcg acccggatcc cgtacggcgc cacctccgcc tggagcgact
     3361 cgctgatccc ctcgaccgcc atcttcgtgg tggcgtacgg cgccatcagc ggcggcgact
     3421 ggatcgccga cgaggaggag atgttcacga tccgccccga ccgcgccgcc cgcatccccg
     3481 gcagcacctc ctggatgcac cgaacaactc cgagcacgtt cgtgtcgaac atcgcctgat
     3541 aggtctcgat cggcgtctcc tcgacggagc ccgcgcccgt gatcccggcg ttgttcacga
     3601 gcacatcgac cggcccatcc gcctggatcg ccgcgaacgc agcatggacg gatgccccgt
     3661 cggtgacgtc gagcggcacc ggacggatcg agtcgagccc cgcatcggcc gccgcctgct
     3721 cgatggcagc gagcccatca gcggagcgag cccccgccca cacctcgaac ccgtcacgag
     3781 ccagtcggtg cgccgtcgcc aacccgatcc ccgagttcgc ccccgtcacc acagcccgct
     3841 tggtcatcgc atcccctcgt gttcgtcgag cgcagcgacc ctatggccta caagaccgcg
     3901 ccggggtggg tgaccttggg tcacgccacc tcctgctgct cgtgcgaaac gagctgatcc
     3961 tcgccgtccg tcggcgcgca catcgacggg cacccgcagg gtccgcgaat ctagtggacc
     4021 ggtagctggt caccgtacgc ccgaccgtgg tgctccacgg ctcgtggcgt cgccttctac
     4081 gacgccgagg tcgaggtcca gaagacccga gactcgggag ctggcaggaa gtgcacgtac
     4141 gacccgttcg acgtactagc tgcgtgcatg ttctctgtca ccggacgttg gacgtttcgg
     4201 ttccgtcgga cgcaagactt gacgccttgg ggcgccgacg ccgagcgggt tggggccatc
     4261 cagcgcatcg acgacagctg ggcgcgctcc gtgcacgatc tggtctcccg acctgagtga
     4321 ccgaagacgt tgccccgttg tcagtcgagc ggggtcagtc cgcgacgcgt tcgaggacgg
     4381 cgacggggat gcggcggtcg gtgagctcct ggtaggtgtc gaagtcgggg tacgccttcg
     4441 ctgccagcgc ccagcactcg tcgcgctcgt cgccttcgac ctcccgggcc gccgtccacc
     4501 accggtcggt accgacctgg acctcggtgc gctcggccgc catcaagttg cggtaccagt
     4561 tcggggtcac tgggttgccg ccgttcgacc cgaccacgat caagtcgtcg ccccgccgaa
     4621 ccagcatcag catcaccggg cgaggctcgc cgctcgtgcg gccgacggcc tccagcagga
     4681 acacgggctt gccgaacatc gcgttgccct tggtcccgcc gctcgagcgg tactgctcga
     4741 cctgcttgta cgtgcccttg gcgacgagcc tcggaaaccg ccggctcatg aagttcgccg
     4801 ctcgtcccgt caggctggcc atcagccgat gctacgacga ccgacgaccg acgaccgacg
     4861 accgacgatt gtcgctccga ggtgagacga cggaccaccg aggttcccgt agcgtccttc
     4921 ccgtgccggc cgccgaggat ccgatcacca tccaggttcg ggtgatcccc cgggccaagc
     4981 gcgacgagat cgacggtgag cgggcgggcc gtctgctcgt ccgcaccacc gcggcacccg
     5041 tcgacg
//
LOCUS       DATMFS010000562         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_19483, whole genome shotgun sequence.
ACCESSION   DATMFS010000562 DATMFS010000000
VERSION     DATMFS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19483"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VNQ33_10640"
     CDS             <1..315
                     /locus_tag="VNQ33_10640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98607.1"
                     /translation="TVMCSIWEGFGLVVAEALYLGRPMVATDVGPVSTMVIAGETGFL
                     VPPSDPSALAAGIDAALADPDHAAELGRAGAKLVSPHFDTGALIARVEDVYDLVTKQP
                     RR"
     gene            312..>2533
                     /locus_tag="VNQ33_10645"
     CDS             312..>2533
                     /locus_tag="VNQ33_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98608.1"
                     /translation="MTRPKTAAHRAAAVALVALAALAPVTGPAAAEPDAPASTGRVLV
                     VSIPRLTWPQIASRDVPNIERFLSQSAVGDLSLRTIGPRTSLGEGYITMGAGNRAGVR
                     EADAGRMLSATDLYENGTAADAYQRRTGWDPDGKLLQLSIAAIQATNDRYLYGAQPGA
                     MGQALTEAGFRTSVIGNDDVELSAAGLVTFDPSSPTGEPSDGPADETAVGDSPDSAIT
                     GTIEPLNGENRPAGLAVMDDQGQVPSGSVSRNLLDRDQNAAFGIALSEEKVLEAFETT
                     WDDRTVSLVELSDLERADLYASRAAAGQARILFDRALTRTDHLLGELLKRTDPGDLVV
                     LVSPASPRAHETLTPVAIRGPGFSSGTLSSGTTRRAGYVTLPDLAPTILDHLGLAKPE
                     PMTGSPIVATNDGTTSPARFHDFIEKNEATKFRDAAATTITVLFVIMQVVLCGLALAA
                     LIRGSVWLRRSAALLGLVTMCLPVVTFAMGLGRLYRIGFWPYLLLMYLGAGLLAWLSL
                     RIGARTDDRRRAVVTTLVPVALTYTLLVVDILVGGHLQLNTIFGYSPLVAGRFSGYGN
                     PAYSLLSMATIILACALWTVFDGDRPGPTRRRLLIGIIAMFCLTIAVDGHPSLGSDVG
                     GVLSIVPSVFLVLWLLLGRRIRIRTVLAAAAATLTAITVFALIDLQRPAAQQTHLGRL
                     VHSTFGDGGGDGLTTTIERKINANITVLTHSIWTWTIPLALIVLARISWRRPRMIDSH
                     L"
BASE COUNT          344 a          983 c          819 g          387 t
ORIGIN      
        1 accgtcatgt gctcgatctg ggagggcttc ggcctcgtgg tcgccgaggc gctgtacctc
       61 gggcgcccca tggtggccac cgacgtcggg ccggtgtcca cgatggtcat cgccggcgag
      121 accgggttct tggtcccccc gtcggatccg tcggcgctcg ccgccgggat cgacgccgcg
      181 ctcgccgacc ccgaccatgc cgctgagctc ggccgggccg gcgcgaagct ggtgagcccg
      241 catttcgata ccggtgccct gatcgccaga gtggaagacg tgtacgacct cgtgaccaag
      301 cagccccgcc gatgacgcgc ccgaagaccg ccgcccaccg cgccgcggcg gtcgcgctcg
      361 tcgcgctcgc cgccttggcg ccggtgaccg gccctgccgc cgccgagccc gacgcccccg
      421 catccaccgg acgggtgctg gtcgtgagca ttccccggct cacgtggccg cagatcgcca
      481 gccgcgacgt gccgaacatc gagcgcttct tgtcgcagag cgcggtcggg gacctgagcc
      541 tgcgcaccat cggcccgcgc acctccctgg gtgagggcta catcacgatg ggggcgggca
      601 accgcgccgg ggttcgtgaa gcggatgccg gccggatgct gtctgccacc gacctgtacg
      661 agaacggcac cgctgccgac gcgtaccagc gccgcacggg ctgggatccc gacggcaagc
      721 tgctccagct gtcgatcgcc gccatccagg ccaccaacga ccgctacctg tacggcgcgc
      781 agccgggcgc gatggggcag gcgctgaccg aggccgggtt ccgcaccagc gtcatcggca
      841 acgacgacgt ggagctcagc gccgccggct tggtcacgtt cgacccgtca tcgcccacag
      901 gcgaaccgag cgatggtccg gcggacgaga cggcggtcgg cgacagcccc gactccgcca
      961 tcaccggcac catcgagccg ctgaacggcg agaaccgccc ggccggcctg gcggtcatgg
     1021 acgaccaggg gcaggtcccg agcggctcgg tctcgcgcaa cctgctcgac cgcgaccaga
     1081 acgccgcgtt cgggatcgcc ctctcggagg agaaggtcct cgaggcgttc gagaccacct
     1141 gggacgaccg gaccgtgagc ctggtcgagc tcagcgacct cgagcgcgcc gacctgtacg
     1201 ccagccgggc tgccgccgga caggccagga tcctcttcga ccgggcgctc acccgcaccg
     1261 accacctcct gggcgagctc ctgaagcgca cggacccggg cgatctggtg gtcctggtgt
     1321 cgccggcatc ccctcgggcg cacgagacgc tcacgcccgt cgccatccgc ggccccgggt
     1381 tctcctcggg cacgctgagc agcggcacca cccgacgcgc cggttacgtg acgctgccgg
     1441 atctcgcgcc caccatcctc gaccacctcg gcctggccaa gccggagccg atgaccggtt
     1501 cgccgatcgt cgccaccaac gacgggacca cctccccagc gcggttccac gacttcatcg
     1561 agaagaacga ggccaccaag ttccgcgatg cggcggccac cacgatcacc gtcctgttcg
     1621 tcatcatgca ggtcgtcttg tgcggcttgg cgctcgccgc gctcatccga gggtcggtct
     1681 ggctgcggcg ctcggcggcg ctcctcgggc tggtgacgat gtgcctgccg gtcgtcacgt
     1741 tcgccatggg gctgggccgc ctctaccgga tcgggttctg gccctacctg ctgctgatgt
     1801 acctcggtgc cggtctgctg gcgtggctgt cgcttcggat cggggcccgc accgacgacc
     1861 gccgtcgagc cgtggtcacc accctggtcc ccgtcgccct cacctacacg ctgctcgtgg
     1921 tcgacatcct cgtgggcggc cacctccagc tcaacaccat cttcgggtac tcgccgctcg
     1981 tggccgggcg gttctcgggg tacggcaacc ccgcctactc cctgctctcg atggccacca
     2041 tcatcctggc gtgcgcgctg tggacggtct tcgacggcga ccgcccgggt ccgacccgcc
     2101 gccggctgct catcggcatc atcgccatgt tctgcctcac catcgcggtc gacgggcacc
     2161 cgtcgctcgg ctccgacgtg ggcggtgtgc tgtccatcgt cccgtcggtg ttcctcgtgc
     2221 tctggctgct gctcggtcga cgcatccgga tccggacggt cctggccgcc gccgccgcca
     2281 ccctgacggc gatcaccgtg ttcgccctca tcgacctcca gcgcccggca gcccagcaga
     2341 cccacctcgg ccggctggtc cactccacgt tcggcgatgg cggcggtgac ggcctcacca
     2401 ccaccatcga gcggaagatc aacgccaaca tcaccgtgct cacccactcg atctggacct
     2461 ggaccatccc gctggcgctc atcgtgctgg cccgcatcag ctggcgccga ccccggatga
     2521 tcgactcgca cct
//
LOCUS       DATMFS010000563         8402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_205054, whole genome shotgun sequence.
ACCESSION   DATMFS010000563 DATMFS010000000
VERSION     DATMFS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205054"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="VNQ33_10650"
     CDS             complement(<1..1856)
                     /locus_tag="VNQ33_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWJ98609.1"
                     /translation="MQSAVDLILVVVVVTAAGGAARRWNKSMPLMLLGLGWLASFLPR
                     FTDFHLEPDVVLVGLLPPLLYAAAVRTSLIDFRSSLPVIGSLSVGLVIATTLGVGAVA
                     GWLLPVSAAAALAIGAVVAPPDAVAATTIARRVGLPRRVVTVLEGESLVNDATALVCL
                     TAATTAIEGHITALEITRDFAISVVGGIGAGLLVAMVVARVQTHVADTVTLTAISLVT
                     PFAAYLLAEEIHASGVLAVVVAGLILGHKAELLQDAPSRLFNRSNWATITFLLEGVVF
                     LLIGLEIRPIVEAVGSSDLSTERIALAAAAVFAAVVVIRFAWVFATAWMLSRRDDPEA
                     LSPSETTVVSWAGMRGVVTLAAAFILPPTTPERDVLILLALVVTAGTLIIEGTTLPIV
                     IRRLAIPGPDRHEDALIAADVLERAGRAGRARLDEELATDPGAAGPEIIERLRRRGTE
                     RAEAMWERLGGTGETPSATYARLRQAMLDAERAEILRIRQTGRVPHEVLQEVLGALDV
                     EESVLALSTSDSTADRAEDLAAPVHPSKQCEHLTEASERASADPVTPDGCEECLRDGD
                     TWVHLRLCLACGHVGCCDSSPNRHAEAHFHETDHPVIRSFEVGEAWRWCYVD"
     gene            complement(1964..2383)
                     /gene="mce"
                     /locus_tag="VNQ33_10655"
     CDS             complement(1964..2383)
                     /gene="mce"
                     /locus_tag="VNQ33_10655"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWJ98610.1"
                     /translation="MFTHIDHVGIAVPDLDEALAFYAEKFDLTSVHEEVNEEQGVREA
                     MLAVGDSGSCIQLLAPLKEDSPIGKFLAKSGPGIQQMAYRVDDIDEVCATLRDRGVRL
                     LYEVPKNGTAGSRVNFIHPKDAGGVLVELVEPGPDPH"
     gene            complement(2436..2867)
                     /locus_tag="VNQ33_10660"
     CDS             complement(2436..2867)
                     /locus_tag="VNQ33_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ98611.1"
                     /translation="MDAFDADVLIYAISPSGPHGPALRSALAETAATTPDGKIGVGSV
                     LLITETLIKPVRGGDDREAGEMARLLDRLELFGCDLATAELAVDLGARYGLKAVDAVH
                     LATAITADADRFITNNRRDFSKAIEEIDITYPEDLVAGSAG"
     gene            complement(2872..3168)
                     /locus_tag="VNQ33_10665"
     CDS             complement(2872..3168)
                     /locus_tag="VNQ33_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWJ98612.1"
                     /translation="MSMTVTVTEARADLPKLLDAVEGGHEVTITRHGKPVAVLMSKDA
                     QRRHRTARAFQGAARVHKMAEDARGKPLFGGPGVSQEYAEEWVDEIRARRDAEG"
     gene            3400..5229
                     /locus_tag="VNQ33_10670"
     CDS             3400..5229
                     /locus_tag="VNQ33_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98613.1"
                     /translation="MSALTIAESSAVVGELRRARRKRRVASIHWVDALYQVYLTGLVS
                     VVLIVLLSGAIGDEKVSAAGIADVKEYGPAAAGVLVALAVFFGLRSGSRGGPLALERP
                     DVRHVLLSPVDRGVALRGPAWRQLRFLVGVGAAVGAATGQLVVRRLPGNALEWIVFGA
                     LYGVTVVGLGYGCALLASGLKIKHGLASLVGGLLLLWSVGDLLGKAPLSPFTVVGHIA
                     VWPLEPDWLALAGVVLAVVLVLAGMRWVSGISLEAAERRTALVGQMRFAVTLQDLRTV
                     LVLRRQLSQEHPRSRPWIRAARRTPKFPVWHRGVRSVARWPLSRIVRGLLMAAVAGFS
                     MRAVWNGTTPLIAVAGMALWIAALDAAEPLGQEVDHPGRTETFPFPRGQVFTLHLPIL
                     FVMSTVIGLLGAAVAAAPLGASVPVGPAVFTGVMAGILAASGAAISVVQGAPDPVDTL
                     SMVTPEIAGTRTVIRTALPPAIAVLGITPLLAARASFLGKQDPPPLNAAMALALPLAI
                     VVVLVAGYVRFRDEIRTFMAEASENMSPTKAVERQREERLAAEAREAEALAESKRRAR
                     EATSASQRAKAKQKPKSKPVSPPPEGVRGGTSAKPIGRKRDQK"
     gene            5226..5915
                     /locus_tag="VNQ33_10675"
     CDS             5226..5915
                     /locus_tag="VNQ33_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98614.1"
                     /translation="MSAAQPPALVAKDLTKTYDDLVALQTMSIEVPSGQSMALIGHNG
                     SGKSTFLRMVAGLLDPTGGEIEIAGFDLTDVEARATTSFLPDDPVLYDDLSLREHAEY
                     ISKLHGGDGWDDYAESLCHRLGLEDRIDDLPSRFSRGLRQKTAIVLALVRPFSLLLVD
                     EPFVGLDQPGKLVLLDLLAEVHAEGAATLVATHDPTYVERVDRSVALRDGEVIFDGQA
                     SVEEVLHLVGG"
     gene            complement(6063..6914)
                     /locus_tag="VNQ33_10680"
     CDS             complement(6063..6914)
                     /locus_tag="VNQ33_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWJ98615.1"
                     /translation="MTATAARSTADEHLPLLIGGHPSLAARARSAAVYVLVLVIGVAA
                     FLYPFWLPSSALPSQAHAGDAPLVAAVVGALAVGAVALEIRRGTMTGTTVAVLGMLSA
                     LAGLMKLLDLPGGGSGLFFLVILAGAAFGPRFGMILGITSFVVSAILSGGFGPWLPFQ
                     MLALGWMGAGAGFVGIATKRMRPWSEVGLLALYGWAWGFVFGAILNLWFWPFQRGGAL
                     DWQPGMSLAESFDRYWAFYVATSLAWDAAGALANAVIILIVGKPLMATLRRYANRLDP
                     ITELAPA"
     gene            complement(6911..>8402)
                     /locus_tag="VNQ33_10685"
     CDS             complement(6911..>8402)
                     /locus_tag="VNQ33_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98616.1"
                     /translation="SGSGKSTLLRTVNGLVPHSSGGRFTGDVVAGGRSITTHRPRNLA
                     DVVGFVHQTPEAQFVVDHVEHDIAFVLENLGSDAALMRRRVEEVLDALGVAHLRDRSP
                     ATLSGGERQRCAVAGALAAGPQVLVLDEPTSMLDPQGADDVMAALRRLNDDLGTTVLL
                     AEHRLDRAGPLADRALLVDAGRVTADGRPEDVLASLASAPPVTHLGRLLGWDPPPLTV
                     RGARDAARRVPLPDAVTAAQAAPPPPVPEPTGDVLLAAAGVTVSHGPRTVLRPTDLAV
                     RQGEIVAVLGRNGSGKTTLLRALAAADAPTGGTVDASVPVAYVPQEPDSLLFSSTVRA
                     EVEATLGLLGRRDPDAVHRWLRTLRLEDLADRHPRSLSVGQRQRVAIAAVAVGDAPVL
                     VLDEPTRGIDATSRHALEHALREHAAGGGAVVLATHDIELAARVATTVLVLGDGDVVA
                     HGDARTVLAGSLFAPQVLRVLPPLLTVDEVGAALAASIADETERAP"
BASE COUNT         1123 a         2881 c         3255 g         1143 t
ORIGIN      
        1 tcgtccacgt agcaccagcg ccaggcctcg ccgacctcga agctgcggat cacggggtgg
       61 tccgtctcgt ggaagtgggc ctcggcgtgg cggttggggc tggagtcgca gcagccgacg
      121 tgaccgcagg cgaggcagag acggaggtgc acccaggtgt cgccgtcgcg gaggcactcc
      181 tcgcagccgt cgggcgtgac cggatcggcg ctggcgcgct cggatgcctc ggtgaggtgc
      241 tcgcactgct tcgacgggtg gacgggcgcg gccaggtcct ccgctcggtc ggcggtggag
      301 tccgacgtgc tgagggcgag cacgctctcc tccacgtcga gggcgccgag cacctcctgc
      361 agcacctcgt gcgggacgcg cccggtctgg cggatgcgca ggatctcggc ccgctcggca
      421 tcgagcatcg cctggcggag gcgggcgtag gtggcgctgg gggtctcgcc ggtaccgccg
      481 aggcgctccc acatggcctc ggcgcgctcg gtgccgcgcc gtcggagccg ttcgatgatc
      541 tccgggccgg cggcgccggg gtcggtggcg agctcctcgt cgaggcgggc gcgcccggct
      601 cgaccggcgc gttcgagcac atcggcggcg atgagcgcgt cctcgtgacg gtcgggcccg
      661 gggatggcga ggcggcggat gacgatgggc agggtggtgc cctcgatgat gagggtgccg
      721 gccgtgacga cgagcgcgag gaggatcagc acgtcgcgct cgggggtggt gggcggcagg
      781 atgaacgccg cggccagcgt gacgacgccg cgcatgccgg cccacgacac gaccgtcgtc
      841 tcgctgggtg acagggcctc ggggtcgtcg cggcggctga gcatccaggc ggtggcgaac
      901 acccaggcga accggatcac gacgacggcg gcgaacacgg cggcggcagc cagggcgatg
      961 cgctcggtgc tgaggtcgct gctgccgacc gcttcgacga tcggccggat ctccaggccg
     1021 atgagcaaga agaccacgcc ctcgagcagg aacgtgatcg tcgcccagtt cgaccggttg
     1081 aacagccggg agggagcgtc ctggagcagc tcggccttgt gcccgaggat gagcccggcg
     1141 acgacgacgg ccagcacccc ggaggcgtgg atctcctcgg ccaggaggta ggcggcgaag
     1201 ggggtcacga gcgagatggc ggtgagcgtc acggtgtcgg cgacgtgggt ctggacccgg
     1261 gcgacgacca tcgccacgag gaggccggcg ccgatgccgc ccaccaccga gatggcgaag
     1321 tcgcgggtga tctcgagcgc cgtgatgtgg ccctcgatcg ccgtggtggc cgcggtgagg
     1381 cacaccagcg cggtggcgtc gttgaccagg ctctcgcctt cgagcacggt cacgacccgc
     1441 cggggcaggc cgacgcgccg agcgatcgtg gtggcggcca ccgcgtcggg cggggccacg
     1501 acggcaccga tggcgagggc cgccgcggct gacacgggga gcagccaccc cgccacggcg
     1561 ccgaccccga gggtggtggc gatgacgagg ccgaccgaca gcgagccgat gaccggcagg
     1621 ctgctgcgga agtcgatgag cgaggtgcgg acggcggcgg cgtagagcag cggtggcagg
     1681 aggccgacca ggacgacgtc gggttcgaga tggaagtcgg tgaagcgcgg gaggaacgag
     1741 gcgagccagc cgaggccgag gagcatcagc ggcatcgact tgttccaccg gcgcgctgca
     1801 ccgcccgcgg cggtgaccac gacgacgacg aggatcaggt cgacggccga ctgcatcgct
     1861 caatcatggc gtggcggtgc ccgcgcgccg accggtggga cccctccccc cgatttcggg
     1921 tcacgcgttc accggtgggg tgctcaggtg acccgaaagg tggtcagtgg gggtcggggc
     1981 cgggctcgac gagctcgacg aggacaccac cggcgtcttt ggggtggatg aagttgacgc
     2041 gggagccggc ggtgccgttc ttcgggacct cgtagaggag gcggacgccc cggtcgcgca
     2101 gggtggcgca gacctcgtcg atgtcgtcca cccggtaggc catctgctgg atgccggggc
     2161 ccgacttggc gaggaacttg ccgatgggcg agtcctcctt gagcggggcg aggagctgga
     2221 tgcagctgcc tgagtcgccc acggcgagca tcgcctcgcg cacgccttgc tcctcgttga
     2281 cctcttcgtg caccgacgtg aggtcgaact tctcggcgta gaacgccagg gcttcgtcga
     2341 gatccgggac ggcgatcccg acgtggtcga tgtgggtgaa catgtccccc attctgccgc
     2401 ggcgtccgcg cttgccgcac gctgcgggcg ggcgatcagc cggcgctgcc ggccacgagg
     2461 tcctcggggt aggtgatgtc gatctcctcg atggccttgg agaagtcccg gcggttgttg
     2521 gtgatgaacc ggtcggcgtc ggccgtgatc gccgtggcca ggtggacggc gtcgacggcc
     2581 ttgagcccgt accgggcgcc gaggtcgacg gcgagctcgg cggtggccag gtcgcatccg
     2641 aagagctcga gacgatcgag gaggcgggcc atctcgccgg cctctcggtc atcccctcct
     2701 cgcacgggct tgatgagcgt ctccgtgatc aacaggacgg acccgacgcc gatcttcccg
     2761 tcgggggtcg tagccgccgt ctcggcgagc gccgagcgaa gcgcggggcc gtgagggccg
     2821 ctcggtgaga tcgcgtagat gaggacgtcg gcatcgaagg cgtccacgac gtcagccttc
     2881 ggcgtcgcgg cgagcgcgga tctcgtcgac ccactcctcg gcgtactcct gcgacacgcc
     2941 gggccccccg aagagcggtt tgcctcgggc gtcttccgcc atcttgtgca cgcgggccgc
     3001 gccctggaag gcccgcgcgg tgcggtgccg gcgctgcgcg tccttgctca tgagcacggc
     3061 gacgggcttg ccgtggcggg tgatggtgac ctcgtggccg ccctccaccg catcgagcag
     3121 cttgggaagg tcggcgcggg cctcggtgac ggttacggtc atgctcatcc tctgtatcgt
     3181 acctaaccgt acagagacgt acggttaggg gggagaagcc actcggggcg gcgatcatga
     3241 cggtgactgc tggaccgaga cggatgagca gcggctccgg acaccacgat ctcctgggcc
     3301 tgaggctcga ccgcttcgcc tcggtcgcca cgagcggtcg gcggggccga tcggcctcgc
     3361 ctgtggttcg ggcggggcgc ggcagtagcg tcgcaggccg tgtccgcgct gaccatcgcc
     3421 gagtcgtccg ctgtcgtcgg agagctccgg cgcgctcgtc gcaagcggcg ggtggcgtcg
     3481 atccactggg tcgacgccct gtaccaggtg tacctgacgg gcctcgtttc ggtcgtcttg
     3541 atcgtgctgc tctccggcgc catcggcgac gagaaggtga gcgcggcggg gatcgccgac
     3601 gtcaaggagt acgggccggc tgccgctggt gtgctggtcg ccctggccgt gttcttcggg
     3661 ctccgctccg gaagccgcgg cggccctctc gcgctcgaac ggcccgacgt gcgccacgtc
     3721 ctgctgtcgc cggtggaccg gggcgtcgcc ctgcggggcc cggcctggcg ccagctccgg
     3781 ttcctcgtcg gcgtgggtgc cgcggtcggg gccgccaccg gccagctcgt cgtccgccgc
     3841 ctcccgggga acgccctcga gtggatcgtg ttcggtgcgc tctacggcgt cacggtcgtc
     3901 gggctcggct acggctgcgc gctgctcgcc tcgggcctca agatcaagca cgggctggcc
     3961 tcgctcgtgg gcggcctgct cctgctctgg tccgtcggcg acctcctggg caaggcgccg
     4021 ctctcgccgt tcaccgtcgt cggccacatc gccgtgtggc ccctggagcc ggactggctc
     4081 gcccttgccg gcgtcgtcct cgccgtcgtg ctcgtgctgg ccgggatgcg ctgggtttcg
     4141 gggatctcgc tcgaagcggc cgagcggcgg accgccctcg tcggccagat gcgcttcgcc
     4201 gtgacgctgc aggacctgcg cacggtgctg gtcctgcgcc ggcagctctc gcaggagcac
     4261 ccccggtcgc ggccgtggat cagggctgct cggcgcacgc cgaagttccc ggtgtggcac
     4321 cgcggcgtgc gcagcgtcgc ccgctggccg ctgagccgca tcgtccgggg cctgctgatg
     4381 gcggccgtcg ccggcttctc gatgcgagcg gtctggaacg gcacgacgcc gctgatcgcc
     4441 gtggccggca tggcgctgtg gatcgcagcc ctcgacgccg ccgagccgct cggccaggag
     4501 gtcgaccacc ccggtcgcac cgagaccttc ccgttccccc ggggccaggt gttcaccctc
     4561 cacctcccga tcctcttcgt catgtcgacg gtcatcggcc tgctcggcgc cgccgtggcg
     4621 gccgcgccgc tcggcgcctc ggtgcccgtc ggtcccgccg tgttcaccgg cgtgatggcc
     4681 ggcatcttgg ccgcctccgg tgccgcgatc agcgtcgtgc aaggcgcgcc ggatccggtc
     4741 gacacgctgt cgatggtgac gccggagatc gccggcaccc ggaccgtgat ccgcaccgcg
     4801 ctgccgcccg ccatcgcggt gctcggcatc acgccgctgc tcgctgcccg cgcctcgttc
     4861 ctcggcaagc aggacccgcc gccgctgaac gccgccatgg cgctcgccct cccgctcgcc
     4921 atcgtggtgg tgctggtcgc cggttacgtg cggttccgtg acgagatccg gacgttcatg
     4981 gccgaggcgt cggagaacat gtcgcccacc aaggccgtcg agcgccagcg cgaggagcgc
     5041 ctggccgccg aggcccgcga ggccgaggcg ctcgccgagt ccaagcgccg ggcccgggag
     5101 gccaccagcg cctcgcagcg ggcgaaggcc aagcagaagc cgaagtccaa gcccgtcagc
     5161 ccgccgcccg agggtgtgcg cggcggaacc agtgccaagc ccatcgggcg gaagagggat
     5221 cagaaatgag cgccgcgcag cctccggccc tggtggccaa ggacctgacc aagacctacg
     5281 acgacctggt cgccctccag accatgagca tcgaggtgcc gtcgggccag tcgatggcgc
     5341 tcatcggcca caacggctcg ggcaagtcca cgttcctgcg catggtcgcc ggcctgctgg
     5401 acccgaccgg cggcgagatc gagatcgccg ggttcgacct gaccgacgtc gaggcccgcg
     5461 ccaccacgtc gttcctcccc gacgatccgg tcctctacga cgacctctcg ctgcgcgagc
     5521 acgccgagta catctcgaag ctgcacggcg gcgacggctg ggatgactac gccgaatcgc
     5581 tctgccaccg gctcgggctc gaggatcgca tcgacgacct gccgtcgcgc ttcagccgcg
     5641 gcctgcgcca gaagaccgcc atcgtcctcg ccctggtgcg gccgttctcg ctgctgctgg
     5701 tcgacgagcc cttcgtcggc ctcgaccagc ccggcaagct cgtcctgctc gatctgctgg
     5761 ccgaggtcca cgccgaaggc gccgccaccc tggtggccac ccacgacccg acctacgtcg
     5821 agcgggtcga ccgcagcgtc gccctccgcg acggcgaggt catcttcgac ggccaggcat
     5881 cggtcgaaga ggtcctccac ctcgtcggcg gctgatctcc gcgggcccgc ccgccgtcgc
     5941 agccccggtc gcatcggcct ctggggtgct gcccgcgggc ctcgatgccg aaggcgtgcg
     6001 cctgcgggtg gggtctgcgt ggccatggcg gccatcgtcg cagcagcggg gctcggctcc
     6061 catcaggcgg gtgcgagctc ggtgatgggg tcgaggcggt tggcgtagcg gcggagggtc
     6121 gccatcaggg gcttgccgac gatgaggatg atcacggcgt tggcgagcgc tcccgctgcg
     6181 tcccaggcga gggaggtggc gacgtagaag gcccagtagc ggtcgaagga ctcggcgagc
     6241 gacatgccgg gctgccagtc gagggcgccg ccgcgctgga acggccagaa ccagaggttg
     6301 aggatggcgc cgaacacgaa gccccacgcc cagccgtaga gggcgagcag gccgacctcc
     6361 gaccacgggc gcatgcgctt ggtggcgatc ccgacgaagc cggcgccggc gcccatccag
     6421 ccgagggcca gcatctggaa cgggagccac gggccgaagc cgcccgacag gatcgccgac
     6481 accacgaacg aggtgatgcc gaggatcatc ccgaacctcg ggccgaaggc ggcgccggcg
     6541 aggatcacga ggaagaacag cccgctgccc ccgccgggca ggtccagcag cttcatcagg
     6601 ccggcgaggg cggagagcat cccgagcacg gcgacggtgg tgccggtcat ggtcccgcgg
     6661 cggatctcca gcgccaccgc gcccacggcc aaggcgccga cgacggcggc gacgagcgga
     6721 gcatcgccgg cgtgggcctg gctgggcagc gccgacgacg gcagccagaa cgggtagagg
     6781 aacgcggcga cgccgatgac gagcaccagc acgtagaccg cagccgagcg ggcgcgagcc
     6841 gccaggctcg ggtggccgcc gatcaggagc gggaggtgct cgtcggccgt ggagcgagcc
     6901 gcggtggcgg tcacggtgcc cgctccgtct cgtcggcgat cgacgccgcg agggcggccc
     6961 cgacctcgtc gacggtgagg agcggcggca ggacccgcag cacctggggg gcgaacagag
     7021 acccggccag cacggtgcgg gcgtcgccgt gggcgaccac gtcgccgtcg ccgaggacga
     7081 ggacggtggt ggcgacgcgg gcggcgagct cgatgtcgtg ggtggcgagg acgacggcgc
     7141 cgccaccggc ggcatgctcc cgcagggcgt gctccagggc gtggcgggag gtggcgtcga
     7201 tgccgcgggt gggctcgtcg agcacgagca cgggcgcatc gcccaccgcg acggcggcga
     7261 tggcgacgcg ctggcgctgg ccgaccgaga ggctgcgggg gtggcggtcg gccaggtcct
     7321 cgagccgcag ggtccgcagc cagcggtgga cggcgtcggg gtctcggcgg cccagcaggc
     7381 cgagggtcgc ctcgacctcg gcgcgcacgg tggagctgaa caggagcgag tcgggctcct
     7441 ggggcacgta ggccacgggc accgaggcgt cgacggtgcc gccggtgggg gcgtcggccg
     7501 cggccagcgc tcggaggagg gtggtcttgc ccgagccgtt gcggccgagg acggcgacga
     7561 tctcgccttg gcggacggcg aggtcggtgg gtcgcaggac ggtgcgggga ccgtggctga
     7621 ccgtcacgcc ggcggcggcg agcaggacgt cgccggtcgg ttcggggacc ggaggcggtg
     7681 gagcggcctg ggcggcggtg acggcgtcgg gcagcgggac gcggcgagcg gcgtcgcggg
     7741 caccgcgaac ggtgagcggt ggcgggtccc agccgaggag gcggccgagg tgggtcaccg
     7801 gcggcgcgct ggccagcgag gccagcacgt cctcgggccg cccatcggcg gtgacgcgcc
     7861 cggcatcgac cagcaaggct cggtccgcga gggggccggc ccggtcgagg cggtgctcgg
     7921 ccagcagcac ggtggtgccg aggtcgtcgt tcagccggcg gagcgcggcc atcacgtcgt
     7981 cggcaccttg cgggtcgagc atggaggtcg gctcgtcgag cacgaggacc tgcggcccgg
     8041 ccgccagtgc gccggcgacc gcgcaccgct ggcgctcgcc gcccgagagg gtggccggcg
     8101 accggtcgcg gaggtgggcc acgccgagcg cgtcgagcac ctcctcgacc cgtcggcgca
     8161 tgagcgcggc atcggacccg aggttctcca gcacgaaggc gatgtcgtgc tcgacgtggt
     8221 cgaccacgaa ctgggcctcg ggggtctggt gcacgaaccc gacgacatcg gccaggttcc
     8281 gcggccggtg ggtggtgatc gaccgaccgc cggccacgac gtcgccggtg aaccggccgc
     8341 cgctgctgtg gggcacgagc ccgttcacgg tgcgcaggag cgtggacttg cccgagccgg
     8401 ac
//
LOCUS       DATMFS010000564         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_114052, whole genome shotgun sequence.
ACCESSION   DATMFS010000564 DATMFS010000000
VERSION     DATMFS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114052"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VNQ33_10690"
     CDS             <1..1057
                     /locus_tag="VNQ33_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWJ98617.1"
                     /translation="DADLVDAAAADETPKGGYGTFPAQVGELQSLLAQKGHTDRPQLW
                     VAHLIAPHMPWVAVPDGTTYAGAVPPGLTVEGGTLTWGQDEAQRRAGYQRLLLQIGAL
                     DREVGAMRQELEQEGIWDDAVVVVTADHGVQFEPGGSRAVGAGGAEVTGVPLFVKAPH
                     QTKGRIDDRAALTMDVVPTVLGQIGLQSPSKFDGLDLFTDRIPDRRTDAFLVGAGSTT
                     TPDQSLDALRKVVERRSKWLDPDGGWDPVFQVGVQQQLVGTSVASLGAATPSDAAWHR
                     TEGETGRRVTIEWDAAPPSSSTALVVCDGTVAGAADTSQAGPVTVFAAPDHCSEPDRG
                     EVWILDSDGAIHQARRS"
     gene            1057..2481
                     /locus_tag="VNQ33_10695"
     CDS             1057..2481
                     /locus_tag="VNQ33_10695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ98618.1"
                     /translation="MEPSPLLEDGGVPAPVPGSFRDPRARVFATDDAILRALNPAGAE
                     DFARLESTSFYGAAQRRGTIVPTRRATGAAADRLKSEGWSEVLLHERIPVVTYPYEWS
                     FSMLQDAALLQLQLVADGLGEGISCKDGTSYNVQFVGSQPTFIDIGSFAPLAGGDAWP
                     GYRQFCSLFLYPLLLESYLDVSFAPWLRGSLEGMTPEVADRLLRGRARMRRGVLTHVT
                     LQAVAARRYAEKDDPAAELASGQESNATIIEALCKRTTALVESLRAPGSATTWSDYSG
                     RSHYGGSSLDEKDRFVREVLAEAEPTTVVDLGCNDGRYAELAAEYATSVVAIDADRRV
                     VDQMYQRLRGRNLPILAMVGDLADPSPGLGWDLTERTPLSERLDADLSLSLALIHHLV
                     IGRNIPIGAYLDALGAIAPRTVLEVPHRDDPMVRRMLAPKPPDTHDDYQLEVIRRLIE
                     ERFEIRRELALSSGTRTIFDLVRR"
     gene            2582..>3124
                     /locus_tag="VNQ33_10700"
     CDS             2582..>3124
                     /locus_tag="VNQ33_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ98619.1"
                     /translation="MTDYTALSDDELLGLARDGDRFAYGELYARHQEDGRRYARRMVG
                     AAADDVLSEAFTKVLGAIHRGHGPVDGFRLYLFTAIRGVAQRSGSQIARLVPMADLDG
                     DHDDDVRLDEMIDSVDGPDRALVLEAFGRLSKSQQEVLWIVDAEQRPPAELVDRFGLS
                     PNAISARAYRARQAFRRAFLE"
BASE COUNT          445 a         1130 c         1095 g          454 t
ORIGIN      
        1 cgatgcggac ctcgtcgatg ccgctgctgc tgacgagacc cccaagggcg ggtacggcac
       61 gttccccgcg caggtcggcg agctccagtc gctcctggcg cagaagggcc acaccgatcg
      121 tccgcagctc tgggtggccc acttgatcgc gccgcacatg ccctgggtgg cagtgcctga
      181 cggcaccacc tacgccggcg ccgtgcctcc gggcctgacg gtggagggcg gcaccctcac
      241 ctggggccaa gacgaagccc agcgccgcgc cggctaccag cggctgctgc tccagatcgg
      301 cgccctcgac cgggaggtcg gcgccatgcg ccaggagctc gagcaggaag gcatctggga
      361 cgacgcggtg gtggtggtca ccgcggacca cggcgtgcag ttcgagccgg gcggctcccg
      421 agccgtcggg gccgggggag ccgaggtcac cggtgtcccg ctgttcgtca aggcgcccca
      481 tcagacgaag ggcaggatcg acgaccgggc ggccctcacc atggatgtcg tccccacggt
      541 cctgggccag atcggcctgc agtcgccgtc gaagttcgac gggctcgacc tcttcaccga
      601 tcgcatcccc gatcgccgca ccgacgcctt cctcgtcggc gcgggttcga ccaccacccc
      661 cgaccagagc ctggacgcgc tccgcaaggt ggtggagcgg cgctcgaagt ggctcgatcc
      721 cgacggaggt tgggacccgg tcttccaggt cggggtgcaa cagcagctgg tcggcaccag
      781 cgtggcgagc ctgggcgcgg cgacgccgtc cgatgccgcc tggcaccgca ccgagggcga
      841 gacgggccgg cgggtgacga tcgaatggga cgcggccccg ccgagcagct cgacggcgct
      901 ggtcgtgtgc gacggcaccg tcgccggagc cgccgacacg tcgcaagccg ggccggtgac
      961 cgtcttcgcg gcgcccgacc actgttctga gcccgatcgt ggcgaggtgt ggatcctcga
     1021 cagcgacggc gccatccacc aagcgaggag atcgtgatgg aaccaagccc gttgctcgaa
     1081 gacggaggcg tgccggcacc ggtgcccgga tccttccggg acccgcgcgc ccgggtgttc
     1141 gccaccgacg atgcgatcct gcgagcgctc aacccggcag gggccgagga cttcgcccgc
     1201 ctcgagtcga cgtcgttcta cggagccgcc cagcggcgcg ggacgatcgt gccgacgcgg
     1261 cgtgcgacgg gggcagccgc cgaccgcctc aagtccgagg gatggtccga ggtcttgctc
     1321 cacgagcgga tcccggtcgt cacctatccg tacgagtggt cgttctcgat gctgcaggac
     1381 gccgcgctgc tccagctcca gctcgtcgcc gacgggctgg gcgaggggat ctcctgcaag
     1441 gacggcacgt cgtacaacgt ccagttcgtg gggtcgcagc cgacgttcat cgacatcggc
     1501 tcgttcgccc ccctggccgg tggcgacgcg tggcccggct accggcagtt ctgctcgctg
     1561 ttcctctacc cgctgctcct cgagtcgtac ctggacgtgt cgttcgcgcc gtggctgcgc
     1621 ggctccctgg aggggatgac ccccgaggtc gccgatcgcc tcctgcgggg gcgggcccgg
     1681 atgcggcgag gcgtgctcac ccacgtcacg ctgcaggcgg tcgcggcacg ccgctacgcc
     1741 gagaaggacg acccggcggc cgagctggcc agcggtcagg agtcgaacgc gacgatcatc
     1801 gaggcgctgt gcaagcgcac caccgctctg gtcgaatcgc tgcgggctcc cggcagcgcc
     1861 accacgtgga gcgactacag cggccggtcc cactacggcg gctcgtcgct cgacgagaag
     1921 gaccgcttcg tgcgcgaggt cctggccgag gcggagccca ccacggtggt ggacctggga
     1981 tgcaacgacg gccggtacgc ggagctggcg gccgaatatg cgacgtcggt ggtggcgatc
     2041 gatgccgatc gccgcgtggt ggaccagatg taccagcgcc tccggggtcg gaacctcccg
     2101 atcctggcca tggtgggtga cctcgccgac cccagccccg gtctcggttg ggatctcacc
     2161 gagcggacgc cgctgagcga acggctcgac gccgacctca gcttgagcct cgcgctcatc
     2221 caccacctcg tcatcgggcg gaacatcccc atcggggcct acctcgatgc cctcggggcc
     2281 atcgcgcccc gcaccgtcct cgaggtgccc caccgtgacg atccgatggt ccggcgcatg
     2341 ttggcgccca agccgccgga cacccacgac gactaccagc tcgaggtcat ccgccgcctg
     2401 atcgaggagc ggttcgagat ccggcgggag ctggcgctct cgagtggcac ccggaccatc
     2461 ttcgacctcg tgcggcgttg accgtcgtcg ggcaccacca ggggtccgtc acgccgagtg
     2521 gctcggtgag ttgtgccttc cggctggctc gatcacccgc atcgcgccgt ccaccacccc
     2581 catgaccgac tacaccgcgc tctccgacga cgaactgctc ggccttgccc gcgacggcga
     2641 ccgtttcgcc tacggcgagc tctatgcccg tcaccaggag gacggccgcc ggtatgcgag
     2701 gcggatggtc ggtgcggccg ccgacgacgt cctctcggag gcgttcacga aggtgctggg
     2761 ggccatccac cgcgggcacg gcccggtgga cgggttccgg ctctacctct tcaccgcgat
     2821 ccgcggcgtc gcgcagcggt ccggctcgca gatcgcccgt ctggtgccga tggccgacct
     2881 cgacggcgac cacgacgacg acgtccggct cgacgagatg atcgactcgg tcgacggccc
     2941 cgaccgtgcc ctggtgctcg aggcgttcgg ccggttgagc aagagccagc aggaggtgct
     3001 ctggatcgtc gatgcggaac agcgcccgcc ggcggagctg gtcgatcgct tcggcctctc
     3061 gccgaacgcc atctcggcgc gggcctaccg cgcccgacag gcgttccgac gggcgttcct
     3121 cgag
//
LOCUS       DATMFS010000565         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_205132, whole genome shotgun sequence.
ACCESSION   DATMFS010000565 DATMFS010000000
VERSION     DATMFS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205132"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VNQ33_10705"
     CDS             <1..690
                     /locus_tag="VNQ33_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98620.1"
                     /translation="ASTGNPQQANGSSVRAGYLEAGRVYTSAGREIGSLATGGVQWDQ
                     LTPVDAGIPAAAEGDDTDVWTADIVATGTDEVTAVLTVTHPDPSPAGGAHSQEYRYAR
                     WTGTRWEVRHLAWGGSELYAGQPSYSGLAVIDPADPERVYLSSNVDPRTGLERRSASD
                     GRSHWEIYEATSTAPGWSISAVTADSTADNIRPVVARGTAGFALAWMRGTYTDFHTYD
                     TGIVVITTTAD"
     gene            complement(717..1475)
                     /locus_tag="VNQ33_10710"
     CDS             complement(717..1475)
                     /locus_tag="VNQ33_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98621.1"
                     /translation="MERSVRLLLAALALVTAAGSVTACQPPTGPRVLIFGDSITIESK
                     GSGDFASILAGYRVDWTGTMYMSAPCNGIALAKQVTYVPDVVIINYAGNSGSFKDNCM
                     AGEKDQALATRYRKDVQALVSKYRNGKTKIVIVGAPARKPTLAQSNLVFTTLQSLAAD
                     PANQVGFFDGGRLITPNRSAPARAAACLSPRETNGRCGTAGAGKNTIRDAQLEHLCPT
                     GGTIGGTCGTYSSGAVRLTLNFRDAIPKAKVAKR"
     gene            1551..2048
                     /locus_tag="VNQ33_10715"
     CDS             1551..2048
                     /locus_tag="VNQ33_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98622.1"
                     /translation="MGPVGHQRIAQRFRVATTPIRWQVPKRKGLDARSKPVMTEAAVI
                     ELSVMGAAIVCPVKWRAVTGSKVQIAWEGVSGFVSIRREVPFPGSSKLAMYGVEFADN
                     PSLVGKALFDHLVAGPAAAAGARVAAVAAEAVIGGYDAAPAHDPGQPAMWPAPVDRSS
                     GLDRP"
     gene            complement(2055..>3252)
                     /locus_tag="VNQ33_10720"
     CDS             complement(2055..>3252)
                     /locus_tag="VNQ33_10720"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWJ98623.1"
                     /translation="EGLHDVAAHETPDGSMAIVGHRPGPEGSPTVLLYFHHDVQPPLD
                     DDAWNTPVWELTDKDGRWFGRGAADCKGNIAMHLTALRALGDDLPVSIKIIGEGSEEQ
                     GTGGLEAFVPLNADLLRADAILVCDAGNFAVGTPSVTTSLRGLANVVVTVKALGSPMH
                     SGMFGGAAPDALAALIAVLASLRDERGNTTIDGLETTTQTWAGADYPSEQFAVDANVL
                     DGVDLLGDGRVSDVVWARPAATVLGIDCPPVVGSSAAIQPEARARINLRVPPGMDAKA
                     AQAALIAHLEAHAPWHVQVTVEPEADGQPFVGAESGPAFDTMAEAMLAVYGKEVTKEG
                     QGGSIPLCNVFQETFPEAEIMLIGVEEPLCLIHAPNESVDPSEIANMAAVEALFLRSY
                     AGHRAR"
BASE COUNT          446 a         1115 c         1220 g          471 t
ORIGIN      
        1 gcgtccacgg gcaaccccca acaggccaac gggagctccg tccgggccgg ttacctggaa
       61 gcaggccggg tgtacaccag cgcgggtcgc gagatcgggt ccctcgccac cggaggcgtg
      121 cagtgggacc agctcacgcc cgtcgatgcc ggcatccccg cggcggccga gggcgacgac
      181 accgacgtgt ggaccgccga catcgtcgcc accgggaccg acgaggtcac cgccgtgctc
      241 accgtcacgc accccgaccc gtcgcccgct ggcggggcgc actcgcagga gtaccgctac
      301 gcccggtgga ccggaacgcg gtgggaggtc cggcacctgg cctggggtgg cagcgagctc
      361 tacgcaggcc agccgtccta cagcggtctc gccgtcatcg accccgccga tcccgaacgc
      421 gtctacctct cctcgaacgt cgatccgcgg accgggctcg aacgacgatc cgccagcgac
      481 ggccggtccc actgggagat ctacgaagcc acgtcgacgg caccgggttg gtcgatcagc
      541 gccgtgaccg ccgacagcac cgccgacaac atccgcccag tcgtcgcccg gggcaccgcc
      601 gggttcgcgc tcgcctggat gcggggcacc tacaccgact tccacaccta cgacaccggg
      661 atcgtcgtga tcacgacgac cgcggactga tccggcctgg cgagcgctcc cgatggtcag
      721 cgcttggcga ccttggcctt ggggatggcg tcgcggaagt tgagggtgag gcggacggcg
      781 ccgctgctgt aggtgccgca cgtgccgccg atggtgccgc cggtggggca gaggtgctcg
      841 agctgggcgt cgcggatcgt gttcttgccg gctccggcgg tgccgcagcg gccgttggtc
      901 tcccgtggtg acaagcaggc ggcggcccgg gcgggcgcgc tccggttcgg ggtgatgagg
      961 cggccgccgt cgaagaagcc cacctggttg gccgggtcgg ctgcgagcga ctggagcgtg
     1021 gtgaacacga ggttcgactg ggcgagcgtc ggcttccgcg ccggcgcccc gacgatcacg
     1081 atcttggtct tgccgttgcg gtacttcgag acgagggcct ggacgtcctt gcggtagcgg
     1141 gttgccaggg cctggtcctt ctcgccggcc atgcagttgt ccttgaacga gccgctgttg
     1201 ccggcgtagt tgatgatcac cacatcgggg acgtaggtga cctgcttcgc cagggcgatc
     1261 ccgttgcagg gcgccgacat gtacatcgtc ccggtccagt ccacccggta gccggcgagg
     1321 atcgaggcga agtcgcccga ccccttggac tcgatggtga tcgagtcgcc gaagatcagg
     1381 acccgggggc cggtcggcgg ctggcacgcc gtcaccgagc ccgccgcggt gacgagcgcg
     1441 agcgcggcga gcaagaggcg gaccgaccgt tccatggcgc tccgacagta ctgcggagcc
     1501 atccggcacg aagtggtcag gaacgggtcg gtcgtgccga caggcacgcc atgggtcccg
     1561 tcggccacca gcgcatcgcg caacggttcc gggtggccac cacgccgatc cggtggcagg
     1621 tccccaagcg aaagggcctc gacgcgcgct cgaagccggt catgacggag gcggcggtga
     1681 tcgagctgtc ggtgatgggc gcggcgatcg tgtgcccggt caagtggcgc gccgtgacgg
     1741 ggagcaaggt ccagatcgca tgggaggggg tctcgggctt cgtgtccatc cgccgcgagg
     1801 tcccgttccc cgggtcgtcg aagctggcga tgtacggcgt cgagttcgcc gacaacccgt
     1861 cgctggtcgg caaggcgctg ttcgaccacc tcgtcgccgg gccggccgcg gcagcgggcg
     1921 cccgcgtcgc ggccgtcgct gctgaggcgg tcatcggcgg gtacgacgcg gcgccggccc
     1981 acgatccggg ccaaccggcc atgtggccgg cgccggtgga ccgatcctcc ggtctcgacc
     2041 ggccctgagc tcgctcagcg ggcgcggtgc ccggcgtagc tccggaggaa gagggcctcg
     2101 accgcggcca tgttggcgat ctcgctggga tcgacgctct cgttcggcgc gtggatcaaa
     2161 cagagcggtt cctcgacgcc gatcagcatg atctcggctt ccgggaacgt ctcctggaac
     2221 acgttgcaca gcgggatcga gccgccctgg ccctccttcg tgacctcctt gccgtagacg
     2281 gcgagcatgg cctcggccat ggtgtcgaag gccgggccgg actcggcgcc cacgaacggc
     2341 tggccgtcgg cctcgggctc cacggtcacc tggacgtgcc acggcgcgtg ggcctcgagg
     2401 tgggcgatga gggcggcctg cgccgccttg gcgtccatgc ccggcggcac gcggaggttg
     2461 atgcgggccc gggcctcggg ctggatggcg gccgaggatc cgaccaccgg agggcagtcg
     2521 atgccgagca ccgtggccgc cgggcgggcc cacaccacgt cggacacgcg gccgtcgccg
     2581 aggaggtcga cgccgtcgag cacgttggca tcgaccgcga actgctcgga cgggtagtcg
     2641 gccccggccc acgtctgggt ggtggtctcc aggccgtcga tggtggtgtt gccgcgctcg
     2701 tcgcgcagcg aggcgagcac ggcgatcagg gcggcgaggg catcgggggc ggcgccgccg
     2761 aacatgcccg agtgcatcgg gctgccgagc gccttcaccg tgacgaccac gttggcgagg
     2821 ccccgcaggc tggtggtgac gctcggggtg ccgacggcga agttgccggc gtcgcacacc
     2881 aagatggcgt cggcgcgcag caggtcggcg ttcagcggga cgaacgcctc gaggccgccg
     2941 gtgccctgct cctcggagcc ctcgccgatg atcttgatgc tcaccggcag gtcgtcgccg
     3001 agggcgcgca gcgcggtgag gtgcatggcg atgttgccct tgcagtcggc ggccccccgg
     3061 ccgaaccagc gcccgtcctt gtcggtcagc tcccacacgg gcgtgttcca ggcgtcgtcg
     3121 tcgagcggcg gctgcacgtc gtggtggaag tagaggagca cggtcgggga gccctcgggc
     3181 ccgggccggt ggccgacgat cgccatcgag ccgtccggcg tctcgtgggc ggcgacgtcg
     3241 tggagaccct cg
//
LOCUS       DATMFS010000566         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_322598, whole genome shotgun sequence.
ACCESSION   DATMFS010000566 DATMFS010000000
VERSION     DATMFS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322598"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VNQ33_10725"
     CDS             complement(<1..657)
                     /locus_tag="VNQ33_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWJ98624.1"
                     /translation="MPELPELQAHAERLGEDFGGAELAGFRPITFTALKTYQPDPSSA
                     AGSAVTAFGRRGKYLLMAVGPVTFVIHLMQGGRLRPDEKQAAKPRGGIARWTFADGRA
                     LLLTEPGTERKAGVWVVSGDLEAQPPIEGLGPDADTLDAAGWAAVVSGRSSRLHGVLR
                     DQHAVAGIGRRLANEICHRARLSPFANASKLKSDQVATLAEAVQECIAESLAAERTHD
                     E"
     gene            complement(661..1113)
                     /locus_tag="VNQ33_10730"
     CDS             complement(661..1113)
                     /locus_tag="VNQ33_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWJ98625.1"
                     /translation="MLTFLSDEWISALHEAASDDAGLAERTRDIVLTIEQEVTGGPLG
                     DVRYHVAFHHGAVSVTAGPAPEATVRFHQDYTTAASIAMGHGSAQRAFMTGRLRVGGD
                     LRVLLAHGEVLAQMEDVFASVRARTAPPIVPDIVPEVSTGSVDAASGD"
     gene            1198..2121
                     /locus_tag="VNQ33_10735"
     CDS             1198..2121
                     /locus_tag="VNQ33_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98626.1"
                     /translation="MPDPAQPADPADEQRHAPTGETLWSESWYLDFFDPDQGIGGYVR
                     LGLYPGMQTAWYWACVVGPDRQLATVIDHDVAIPPGRSHEIRSEGLWADYTVETPLDH
                     VSVGVEAFAVGVDDPAEVYGDLRGDRVPLGFDLEWETDGGSYAYPGVTRYEVPCRVHG
                     EVLIGAERFEVDGFGQRDHSWGVRDWWSYGWSWTAGRLDDGTRFHGVDVRLGADPLYG
                     TGYVQAPGAAMDAVDVVSHEAEVDGEGLPVTASWQLGGLDLSIEPVAFSPVLLTADDG
                     RASRFPRAWCRFTAADGRSGHGWTEWNQPQP"
     gene            complement(2106..>3101)
                     /locus_tag="VNQ33_10740"
     CDS             complement(2106..>3101)
                     /locus_tag="VNQ33_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98627.1"
                     /translation="ISVPALLGRTAAVPDRDGGIVLACGEGPPVQVDGATIATQARTT
                     VGALLDGSPIAWEACSPVALGHGTHRVEATAGSLLVSTLVIEPVDGIGPAPAGRSVSV
                     GAWGPSQRTVSIGRGDEAILATTENANDGWEATLDGKVLTPIRVDGWRQGWQVPAGSA
                     GTVHLRYRPATTQRVGLAIALAALLALVAAALVAPRGRGAARTWMPPGERGWPKPAVW
                     ALALLAGVALGGPLVLVAVPLASVPRRTRWLPLLAAGAAVAAGLVTFIWAGADVGDIT
                     GTFSGPAQAFAVTAIVATALALLPEQLSAGATEPPAAPGAEPAAAAAEPPDQGWG"
BASE COUNT          407 a         1140 c         1152 g          402 t
ORIGIN      
        1 ctcgtcgtgg gtgcgctccg cggccagcga ctcggcgatg cactcctgga cggcctcggc
       61 gagcgtcgcc acctggtcgg acttgagctt cgatgcgttg gcgaacgggg acagcctcgc
      121 ccggtggcag atctcgttgg cgagccggcg gccgatcccg gcgacggcgt gctggtcgcg
      181 cagcacgccg tgcaggcgag aggatcggcc cgagacgacg gcggcccacc cggccgcgtc
      241 gagcgtgtcg gcgtcgggac cgagcccttc gatcggcggc tgcgcctcga ggtcgcccga
      301 caccacccag acgcccgcct tgcgctccgt tccgggctcg gtcagcagca gcgcccgtcc
      361 gtcggcgaag gtccaccggg cgatcccgcc gcggggcttc gccgcctgct tctcatcggg
      421 ccggagccgc ccgccctgca tcaagtggat gacgaaggtg accggaccca ccgccatgag
      481 caggtacttc ccccgccgcc cgaaggccgt gaccgctgag cccgcggccg acgacggatc
      541 aggctggtag gtcttgagcg cggtgaacgt gatcggccgg aaccccgcca gctccgcacc
      601 gccgaagtcc tcgccgaggc gctcggcatg ggcttggagc tcgggcagct cgggcatcga
      661 tcagtccccg gacgccgcgt cgaccgagcc ggtgctgacc tcggggacga tgtcggggac
      721 gatcgggggt gcggtccgcg cccgcaccga ggcgaacacg tcctccatct gggccaggac
      781 ctcgccgtgc gcgagcagga cccgcaggtc accgcccacc cgaagccggc cggtcatgaa
      841 cgcccgctgc gctgagccgt ggcccatggc gatggacgcc gccgtcgtgt agtcctggtg
      901 gaagcggacg gtcgcttctg gggccgggcc ggcggtgacc gacaccgcac cgtggtggaa
      961 cgccacgtgg tagcggacgt cgccgagcgg cccaccggtg acctcctgct cgatcgtgag
     1021 gacgatgtcg cgtgtgcgct cggccaggcc ggcgtcgtcg gacgcggcct cgtgcagggc
     1081 gctgatccac tcgtcgctca ggaacgtcag catcggagcc acgctaccgg cgcggcgtcg
     1141 tacgatcgcc gctcggcgtc ggcagcccgt cgtgccgctg tcatcggagg agccctcgtg
     1201 cccgatcccg cccaacctgc cgacccggcc gacgagcagc gccacgcccc caccggcgag
     1261 acgctgtgga gcgagagctg gtacctcgac ttcttcgacc ctgaccaggg gatcgggggc
     1321 tacgtgcgcc tcggcctcta ccccggcatg cagaccgcgt ggtactgggc gtgcgtggtc
     1381 ggtcctgacc ggcagctcgc caccgtcatc gaccacgacg tggcgatccc gcccggacgg
     1441 agccacgaga tccgcagcga gggcctgtgg gccgactaca cggtcgagac cccgctcgat
     1501 cacgtgtcgg tcggcgtcga ggcgttcgcc gtcggcgtcg acgatccagc ggaggtgtac
     1561 ggcgacctgc gcggcgaccg cgtgccgctc gggttcgatc tggagtggga gaccgacggc
     1621 ggctcgtatg cgtatcccgg cgtcacccgc tacgaggtgc cgtgccgggt ccacggcgag
     1681 gtgctgatcg gagccgagcg cttcgaggtg gacggcttcg gccagcggga ccactcctgg
     1741 ggcgtgcgcg actggtggag ctacggctgg agctggaccg cgggccggct cgatgacggc
     1801 acccgcttcc acggcgtcga cgtccgcttg ggcgctgacc cgctctacgg aaccgggtac
     1861 gtgcaggcgc ccggtgcggc gatggacgcg gtcgacgtcg tgagccacga ggccgaggtc
     1921 gacggcgagg gcctcccggt gaccgcatcg tggcagctcg gtggcctcga cctgtcgatc
     1981 gagccggtgg cgttctcgcc cgtgctgctc accgccgacg acggccgcgc cagccgcttc
     2041 ccccgggcgt ggtgccgatt caccgctgcc gacggccgct ccggccacgg ctggacggag
     2101 tggaatcagc cccagccctg atcgggtggc tcggctgctg ctgccgccgg ctcggcgccg
     2161 ggcgcagcag gcggctcggt ggcaccggct gagagctgtt cggggagcag agccagcgcc
     2221 gtggcgacga tggcggtcac ggcgaacgcc tgggccgggc ccgagaaggt gccggtgatg
     2281 tcgcccacgt cggcgccggc ccagatgaac gtgaccaggc cggcggccac ggccgcgccc
     2341 gcggcgagca gcggcagcca ccgggtacgt cggggcacgg aggcgagcgg gacggcgact
     2401 aggacgagcg gtccgccgag cgccacgccg gccagcaggg ccagcgccca caccgccggt
     2461 ttcggccacc cccgctcacc cggtggcatc caggtgcggg ccgcgccacg accccggggc
     2521 gcgaccagcg cggcggccac gagcgcgagc agcgcggcca gcgcgatcgc caggccgacg
     2581 cgctgggtcg tcgcagggcg gtagcggagg tggaccgtcc cggccgaccc ggcggggacc
     2641 tgccacccct ggcgccagcc gtcgactcgg atcggcgtca gcaccttccc gtcgagcgtc
     2701 gcctcccagc cgtcgttggc gttctcggtc gtggcgagga tcgcctcgtc gccacggccg
     2761 atggagacgg tccgctggga cggaccccag gcgccgaccg acacggatcg gcccgccggt
     2821 gccggtccga tcccgtcgac cggctcgatc acgagtgtgg acacgagcag cgagcccgcc
     2881 gtcgcctcga cccggtgcgt gccgtggccg agggcgacgg gcgaacacgc ctcccaagcg
     2941 atcgggcttc cgtcgagcag cgctccaacg gtggtcctcg cctgggttgc gatcgtcgca
     3001 ccgtcgacct gcaccggcgg gccttcgccg cacgccagca cgatcccgcc gtcgcggtcg
     3061 gggaccgcag cggtgcgccc gagcagcgcc ggcaccgaga t
//
LOCUS       DATMFS010000567         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_179463, whole genome shotgun sequence.
ACCESSION   DATMFS010000567 DATMFS010000000
VERSION     DATMFS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179463"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VNQ33_10745"
     CDS             <1..345
                     /locus_tag="VNQ33_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98628.1"
                     /translation="AELADHAIGVSAICPGFVDTGIATATRYVGATDAEQDERRRSAD
                     RMYKRRRVTPDAVADAVLRAVDGNRPLVPVAAEARLGLLAQRFAPHLRRAIARIDLTP
                     PAPTRRRTEDPS"
     gene            342..1208
                     /locus_tag="VNQ33_10750"
     CDS             342..1208
                     /locus_tag="VNQ33_10750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWJ98629.1"
                     /translation="MTAIVPRRVRFDWADTPLHWVPGDPQTTHTINVLHLLLPAGEKW
                     FVHVYRQALPLVTDDQLRAGVKGFMGQEAVHSRAHAGVLDHLAEQGLDPTPYTRHVDW
                     LFERLLGDEPLGRTPPAWMQRAWLKQRLAIIAAVEHYTAVLGMWVLDSPGLDAAGADP
                     TMLDLLRWHGAEEVEHRSVAFDLFQHVSGSYIRRVETMAVSALALAFLWIQGTRFFMA
                     ADPTVRGKATWRAFRRAVRQGRAPSLRQIYGTIPDYLRRSYHPGAHGDTALAMAYLAA
                     SPAARAAAGETV"
     gene            1205..2026
                     /locus_tag="VNQ33_10755"
     CDS             1205..2026
                     /locus_tag="VNQ33_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWJ98630.1"
                     /translation="MTEADRPALRAMGPAGARELRIDDLAREAGTTVRNVRAYQDRGL
                     LAAPRREGRVAWYGEGHLVRLRLIGSLLDRGFTLANIAELLDGWSEGRNLGDLLGLGK
                     GLAGPFSDEVPDTGPPAEIASRYGLDVDDPAAASDALLLGLIEIDGDQLRVPSPRLLR
                     AGVELHDAGVPLPDLFAELRRIRTDVEDMAERFVRLVVAHVFEPHLVDGMPAPDKVPE
                     LADQMARIRPLASIVVEAELVRALQARADAELGARLTALLARRGADGGRAAGSAG"
     gene            complement(2015..2398)
                     /locus_tag="VNQ33_10760"
     CDS             complement(2015..2398)
                     /locus_tag="VNQ33_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWJ98631.1"
                     /translation="MATIDLTEATFPGTIAEGTVLVDWWASWCGPCRAFAPTYEAVSE
                     QHPDITFAKIDTEAEQGLAGAADIQSIPTLMVFRDGILLFRQSGALPAAALDDLVSQV
                     QALDMDEVRAQVAANQMEGDPASAG"
     gene            complement(2502..3362)
                     /locus_tag="VNQ33_10765"
     CDS             complement(2502..3362)
                     /locus_tag="VNQ33_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HWJ98632.1"
                     /translation="MAAPPPRRRNDGILSDPIDLDGPRNPRAQHPAVPARPGLVVKQR
                     GTPVTGIVVGVVNGYLHVKDRQGFEHRMTMLKGGFEVDGRIVTLVEPRGVAPGTPAAA
                     PPRTASGSVAVPNAPAQVAKASRILVEGRHDAELVEKVWGDDLRVEGVVVEQLEGADH
                     LDDVVRSFAPKPGRRLGILLDHLVDGSKETRIAASVAHPHVLVTGHPYVDVWQAVKPS
                     VLGIAAWPEVPRGEPWKEGVMRRLGVEGETWQFWKQLLGRVTTWTDLEPPLIGAVEEL
                     IDFVTEPAGA"
     gene            3481..>3923
                     /locus_tag="VNQ33_10770"
     CDS             3481..>3923
                     /locus_tag="VNQ33_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWJ98633.1"
                     /translation="MSTRAWSVTPSGPLRGDVFIRGSKNAVTKHMVAAILGNGVSTIR
                     QVPEVGDVAITADILRSLGVAVEHDEAAGVISVEPHEGVTTRVPASFSGLNRIPILLL
                     GPLLHRTGEAFVPLVGGDPIGRRPVDFHIEALTALGAEIEVTDEGL"
BASE COUNT          492 a         1498 c         1397 g          536 t
ORIGIN      
        1 gccgagctcg ccgaccacgc catcggggtc agcgccatct gccccggctt cgtcgatacg
       61 ggcatcgcca ccgccacccg ctacgtcggc gccaccgatg ccgagcagga cgagcgccgc
      121 cggtccgccg accggatgta caagcgccgg cgggtcacgc ccgacgcggt ggccgacgcc
      181 gtcttgcggg cggtcgacgg caaccggccg ctcgtcccgg tcgcggccga ggcccggctc
      241 ggcctgctgg cccagcggtt cgccccccac ctgcgccggg ccatcgcccg catcgacctc
      301 accccgcccg cgcccacccg ccgccgaacg gaggacccct cgtgaccgcc atcgtccccc
      361 gccgggtccg cttcgactgg gccgacacgc cgctgcactg ggtgcccggc gacccgcaga
      421 ccacccacac catcaacgtc ttgcacctgc tgctcccggc gggggagaag tggttcgtgc
      481 acgtctaccg ccaggccctg ccgctggtca ccgacgacca gctccgagcc ggtgtgaagg
      541 ggttcatggg ccaggaggcg gtgcactccc gagcgcacgc cggcgtgctc gaccacctcg
      601 ccgagcaggg gctggacccg acgccctaca cccggcacgt ggactggctc ttcgagcgcc
      661 tcctcggcga cgagccgctc ggccgcaccc cgccggcgtg gatgcagcgg gcgtggctca
      721 agcagcggct cgccatcatc gccgccgtcg agcactacac cgcggtgctc ggcatgtggg
      781 tgctggactc gccggggctc gacgccgccg gagccgaccc cacgatgctc gacctgctgc
      841 gctggcacgg cgcggaggag gtggagcacc gcagcgtggc gttcgacctc ttccagcacg
      901 tctcgggctc gtacatccgc cgggtcgaga cgatggccgt ctccgcgctg gccctggcgt
      961 tcctctggat ccagggcacc cggttcttca tggctgccga ccccaccgtg cggggcaagg
     1021 ccacgtggcg ggcgttccgg cgggccgtcc gccagggtcg agccccgtcg ctgcgccaga
     1081 tctacggcac gatccccgac tacctgcgcc gctcgtacca ccccggcgcc cacggcgaca
     1141 cggccctcgc gatggcgtac ctggccgcct cgcccgcggc ccgggccgca gcaggggaga
     1201 cggtgtgacc gaggccgacc gcccggcgct ccgggcgatg gggccggcgg gagcgcggga
     1261 gctgcgcatc gacgacctcg cacgggaggc gggcaccacg gtccgcaacg tccgcgccta
     1321 ccaggaccgc ggcctgctcg ccgccccccg gcgggagggc cgggtcgcct ggtacggcga
     1381 gggccacctc gtccggttgc gcctcatcgg ctcgctcctc gaccggggct tcacgctcgc
     1441 caacatcgcc gagctcctag acggttggtc cgagggccgg aacctgggcg acctcctcgg
     1501 cctgggcaag ggcctggcag ggccgttcag cgacgaggtg cccgacaccg ggcccccggc
     1561 ggagatcgcc tcgcgctacg ggctcgacgt cgatgatccg gcggcggcgt ccgatgcgct
     1621 gctgctcggt ctgatcgaga tcgacggcga ccagctccgc gtcccgagcc cgcgcctgct
     1681 gcgcgccggg gtggagctcc acgatgccgg cgtgccgctc cccgacctgt tcgccgagct
     1741 gcggcgcatc cgcaccgatg tggaggacat ggccgagcgg ttcgtccggc tcgtggtcgc
     1801 ccacgtgttc gagccgcacc tcgtcgacgg gatgcccgcc cccgacaagg tgcccgagct
     1861 ggccgaccag atggcgcgga tccgaccgct cgccagcatc gtggtcgagg cggagctcgt
     1921 gcgggcgctc caggcacggg ccgacgccga gctgggcgcc cggctcaccg cgctgctggc
     1981 gcgccggggc gccgacggcg gccgggcggc tgggtcagcc ggctgaggcc ggatcgccct
     2041 ccatctggtt ggcggcgacc tgagcgcgga cctcgtccat gtccagggcc tggacctggc
     2101 tgaccaggtc gtcgagtgcc gcagcaggaa gggcgccgga ctggcggaac agcaagatgc
     2161 cgtcgcggaa caccatgagc gtcgggatcg actggatgtc cgctgcgccc gccaggccct
     2221 gctcggcctc ggtgtcgatc ttggcgaacg tgatgtcggg gtgctgctcg gacacggcct
     2281 cgtaggtggg ggcgaacgcc cggcacggcc cgcaccacga cgcccaccag tccacgagga
     2341 cggtgccctc ggcgatggtg cccgggaagg tcgcttcggt caggtcgatg gtggccatgg
     2401 gatcgctgct ccgtttggtg aggtgctgtg gcttcaacgg cggggcggcc cacggcattc
     2461 cccgtgggcc catccgtgcg gtggtcgggc cggtggcggg atcaggcccc ggccggctcg
     2521 gtcacgaagt cgatcagctc ctcgacggca ccgatgagcg gcggctcgag gtcggtccag
     2581 gtcgtgaccc ggccgagcag ctgcttccag aactgccagg tctcgccctc gacgccgagg
     2641 cggcgcatca cgccctcctt ccacggctcg cctcggggca cctccggcca ggcggcgatg
     2701 cccagcacgc tcggcttcac cgcctgccac acgtcgacgt aggggtggcc cgtcaccagc
     2761 acgtgcggat gcgccaccga ggcggcgatg cgggtctcct tcgatccgtc gaccaggtgg
     2821 tccagcaaga tgccgagccg ccgtcccggc ttcggggcga agctgcgcac cacgtcgtcg
     2881 aggtggtcgg cgccttcgag ctgctcgacc accacgccct cgacgcgcag gtcgtcgccc
     2941 cagaccttct cgacgagctc ggcgtcgtgg cggccctcca ccaggatgcg gctggccttg
     3001 gccacctggg cgggcgcgtt cggcacggcc acggagcccg acgcggtgcg cgggggcgcg
     3061 gcggccggcg tgccgggggc gaccccccgg ggctcgacca gggtcacgat gcggccgtcg
     3121 acctcgaagc cgcccttcag catcgtcatc cggtgctcga agccctggcg gtccttgacg
     3181 tgcaggtacc cgttgacgac gccgaccacg atgccggtga cgggggtgcc gcgctgtttc
     3241 accaccaggc ccgggcgcgc cgggaccgcg gggtgctggg cccgggggtt gcggggtccg
     3301 tcgaggtcga tcgggtccga gaggatgccg tcgttgcggc gtcgaggcgg aggagcagcc
     3361 atcgcccgag ggtaggcgtg gcccgacggc cgagcgtcga ccccgtcctg ccgcggaacc
     3421 ccgcccaggt cgcggtgccc ttgcggccac cggtccgatg ggtgcgtagc cttgaccgcc
     3481 atgagcacgc gtgcctggtc ggtcacaccc tcgggtcccc ttcggggcga cgtcttcatc
     3541 cgcgggtcga agaacgccgt caccaagcac atggtcgccg ccatcttggg caacggcgtc
     3601 agcaccatcc gccaagtccc cgaggtcggc gacgtcgcca tcaccgccga catcctccgc
     3661 tcgctcggcg tcgccgtcga gcacgacgaa gccgccggcg tcatctcggt cgagccccac
     3721 gagggcgtca ccacgcgggt gcccgcgtcg ttctcggggc tcaaccgcat cccgatcctg
     3781 ctgctcgggc ccctgctcca ccgcaccggc gaggcgttcg tgcccctggt cggcggcgac
     3841 cccatcggcc gccgcccggt cgacttccac atcgaggccc tcaccgcgct cggtgccgag
     3901 atcgaggtga ccgacgaagg cct
//
LOCUS       DATMFS010000568         6889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_179534, whole genome shotgun sequence.
ACCESSION   DATMFS010000568 DATMFS010000000
VERSION     DATMFS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179534"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..340)
                     /locus_tag="VNQ33_10775"
     CDS             complement(125..340)
                     /locus_tag="VNQ33_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98634.1"
                     /translation="MAATPVLAYLDAGTGAALAAVVASGAVGARALFGNAKTKVGRKI
                     GRGGSANAAGSSDEASTDASAPANDAS"
     gene            complement(453..2555)
                     /locus_tag="VNQ33_10780"
     CDS             complement(453..2555)
                     /locus_tag="VNQ33_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98635.1"
                     /translation="MLLLIVALALVVGSTALVVSSLGFASRVSAVLAGSVIGLAQVNV
                     SVLIAGAVIHELEPFVLLGVTAVVALASAALALAFASDGIGLFADLLERLPFPARGDV
                     GARVLADPQAARPDPSDSGVEGDRRVLGDADADDSAWPRRWITAAWEAVAGHPFHTIC
                     LVLVAVAYAWHVFLALALPIVDYDGLALHATAVAHWVTSGGFGRSGLSVTLDSAPLGP
                     AAISAWAATFTGSLRWVFATQLAFTAVGVLAVIDLARRFGASLADAVLAGAVFGAVPV
                     VVLQATSAYADLGASTPLLAAWSFGLGAYASASEKPAFGRDRQASRSHGGAGTGQHLV
                     VAGSALGLAVGARPANLAAVAVFVVLVVFGSVWAGRTSEASAATVARSTAVRVGIVLL
                     PVLVIGSYWYLRNWIEFGSPLYPFSIGPFHGLGSVEELIVAPNRPDTLDAVGGNVART
                     ITSWSADLPPGSYAVDQRLGGFGPLFLVALVPALVLFVSRSVRTAPARAIAVVAPAVL
                     LLALQPAPWWARGTIFIPGIAAAAFAAVLPEQRGGVRTGVLAGATVLLGWGLWAVASQ
                     TVYTDAVTHRRVELGDAWSLVHEDDRAHAVLPYSGYAVLDTYPSGTVVAVLDGFVPSQ
                     RAPLYGQDLDRRLVIVPPTSDPDELARSMDQAGADIAVVPVSSESLIEAVRQPGSPLQ
                     IVAERGASPARTVLVAPA"
     gene            complement(2556..3122)
                     /locus_tag="VNQ33_10785"
     CDS             complement(2556..3122)
                     /locus_tag="VNQ33_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HWJ98636.1"
                     /translation="MTALSRPLPSSPRTFSFSPLRAGLGVALIASGALLVQAQADVRV
                     AEAALAAPPLKFVLGEGVHVPGTDLVFYPNYSGHGLRALKVTMSCSSVLVVGPMIAMG
                     GIMAMVRRFSPVRAVVAMGAAAATMFTINLGRIVLIAVATRQFGRDGFEVSHRVVGSG
                     IVLAAATVAFVVAYRIVARYADVDRRSR"
     gene            complement(3119..4513)
                     /locus_tag="VNQ33_10790"
     CDS             complement(3119..4513)
                     /locus_tag="VNQ33_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ98637.1"
                     /translation="MIGALALIIVGLGMAYFLMTTSSGVLFERRRGKEPVTPEGPEPT
                     TVFFLIGCLNEELVIEETVERLLVDDRARVVVVDDGSDDRTSAIAAAVDADRVTVLRR
                     ELPNARLGKGKALNDGFARINEIVHEEDIDPSTVLVCVMDADGHLSRGCLDKVLPLFR
                     DPDVGGAQLIVRIRNRDTLLGLIQDIEFFGLTATCQIGRVATGTVSLGGNGQFARLTA
                     LNGLDGVPWSESLTEDLDLTISMLTNGWKLTTTDEAHVTQQGVEQIRPLIKQRTRWYQ
                     GHMTCGRRLPELWRSRALPHGAVLEVTAYLAVPWLQVLPWSILFHLGIWQTYQTISGK
                     GWEVFGTSVVAQVVGMVLWYLLIFFPNIVNGSLYFRRVRSTGLIKSLALGHLLILAAY
                     ISYAACWRALYRMVVGRTGWDKTRRAQEMASPTLFLGAAAAAAEGRQPVSVGGEAGAG
                     AMASASATTAVGQP"
     gene            complement(4510..5634)
                     /gene="wecB"
                     /locus_tag="VNQ33_10795"
     CDS             complement(4510..5634)
                     /gene="wecB"
                     /locus_tag="VNQ33_10795"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWJ98638.1"
                     /translation="MSSGLVGESRRSVAVVLGTRPEIIKLARIIQLLGDRATVVHTGQ
                     HYDASLSSSFFEEHGLADPDVQLGVGGLRRGGQVGQALADLDGLFDRHRPDVVIVQGD
                     TNATLAGALAANSASIPLFHVEAGLRSYDSAMPEEHNRVLTDHVSDVLCAATKLNADN
                     LALEGLADRAVITGNTVVEAVARQLPAAPDRRGVLERYGCDDAGYVLATIHRPENTDD
                     PEKLGDILRALGACGRQVLLPLHPRTRGAIERHGLQPLTDGLHVVDPLGSRAFLALAN
                     HAALLVSDSGGVQEECTVLKRPLVVVRNSTERPESLERFARLVQPDALLGAITEVLEA
                     GPEHLAELADLPSPYGDGFASERIVAHVLRIADDDREVEA"
     gene            complement(5672..5866)
                     /locus_tag="VNQ33_10800"
     CDS             complement(5672..5866)
                     /locus_tag="VNQ33_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98639.1"
                     /translation="MYNSPPPGAAVAGVIGIGGGTLAVTGFNTLSLGIIAALLIVGGL
                     VLLRIAVLARTERESAAPAT"
     gene            complement(6020..>6889)
                     /locus_tag="VNQ33_10805"
     CDS             complement(6020..>6889)
                     /locus_tag="VNQ33_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98640.1"
                     /translation="LLPATAGAATRTQSDVGASVNCNLNGLHQNQGPVSTCQNLSIVT
                     QILVNGVDVAPGTSLVVGDVVTVRVTVPPRGGHDAYYQETSDGIRNNSRDERFAIRVG
                     LPAETGTSLSTPTNVTMSRTDNGDRGNGPAINCLAQNGQEGARTDPPTGVTVGGPSVG
                     STEMSASVNGATLAAFWDQMAYDRCVNVLSNNGYYAGFTLSFDQIVVTGGAVELTPTW
                     TLGQVTQELKNGSRSIAWGSATDVALHLPAEDPIPVVDPYLFAAVTVASGGGLLVWWR
                     SRRQTAPEPAPAR"
BASE COUNT         1145 a         2378 c         2489 g          877 t
ORIGIN      
        1 cacccgcacc agcgggaacg acaccgcgaa caccgccaag ccgaagaggg cgagcacccc
       61 cgtggaccgg cgatgctcgt cggtccgctc ttcctccatc gcatacgcca tggtccgaag
      121 gtgctcagct ggcgtcgttc gcgggcgccg acgcgtcggt gctggcctcg tccgaggacc
      181 cagcggcgtt ggccgagccg cctcggccga tcttgcggcc taccttcgtc ttggcgttgc
      241 cgaacagagc acgggcgccg acggcacccg aggcgacgac cgccgcgagg gccgcgccgg
      301 taccggcatc gagataggcg aggacagggg ttgcagccat gagcgggctc cttgggtcgt
      361 gaacgggggc atcacacgca ggtacgtccg gccccaccgg gtcgtacaac ccgaagccac
      421 cagggtcgtg acggtggcca gcgaacggag ggtcaagccg gggcgacgag gaccgtgcga
      481 gccggcgacg cgccccgctc ggccacgatc tgcaacgggc tccccggctg gcgcaccgcc
      541 tcgatcagcg actcggagct gaccgggacc accgcgatgt cggctccggc ctggtccatc
      601 gagcgggcga gctcgtcggg atcagaggtg gggggcacga tcacgaggcg gcggtccagg
      661 tcctgcccgt agagcggcgc tcgctgcgac ggcacgaacc cgtccaagac ggccacgacg
      721 gtgcccgagg gataggtgtc gaggaccgcg tacccgctgt aggggagcac cgcgtgggcg
      781 cggtcgtcct cgtgcaccag cgaccacgca tcgccgagct cgacgcggcg gtgggtcacg
      841 gcatcggtgt agacggtttg gctcgcgacc gcccagagcc cccatccgag caggaccgtc
      901 gcgcccgcca gcacgcccgt gcggacgcca ccgcgctgct cggggagcac ggcggcaaat
      961 gcggcggcgg cgatacccgg aatgaagatc gtcccccggg cccaccacgg cgcgggctgg
     1021 agcgccagca gcagtaccgc cggcgcgacg acggcgatcg ccctcgccgg cgcggtgcga
     1081 accgagcgcg acacgaacaa gacgagcgcc ggcaccaggg cgacgaggaa gagcgggccg
     1141 aaccccccga gccgctgatc caccgcgtag gagcccggcg ggagatcggc cgaccacgac
     1201 gtgatggtcc gcgccacgtt cccgccgacg gcgtcgagcg tgtcgggtcg gttcggagcg
     1261 acgatgagct cctccaccga gccgagaccg tggaacgggc cgatcgagaa ggggtagagc
     1321 ggactgccga actcgatcca gttgcgcagg taccagtagc tgccgatcac caagacgggc
     1381 aggagcacga tgccgacgcg caccgcggtg gaccgagcca ccgtcgccgc ggaagcctcc
     1441 gatgtccggc cggcccacac cgaaccgaag acgacgagca cgacgaagac ggctaccgca
     1501 gcgaggttcg caggccgggc accgacggcc aggccgagcg cgctgccggc aaccaccagg
     1561 tgctggcccg tgcccgcgcc tccgtgggag cgcgatgcct gccgatcccg cccgaaggcc
     1621 ggcttctcgg aggcggacgc ataggcgccg aggccgaacg accacgcagc gagcagcggg
     1681 gtcgacgcgc ccaggtcggc gtaggccgac gtcgcttgca gcaccaccac cgggaccgcg
     1741 ccgaagacgg cgccggccag cacggcgtcg gcgagagacg ctccgaagcg acgagcgaga
     1801 tcgatcacgg cgagcacgcc aacggcggtg aaggcgagct gcgtggcgaa cacccagcgc
     1861 agcgaaccgg tgaaggtcgc ggcccaagcc gagatggccg ccggaccgag gggtgcgctg
     1921 tcgagcgtca ccgacagccc cgaccggccg aacccgcccg aggtcaccca gtgggccacg
     1981 gccgtggcgt gcagcgccaa cccgtcgtag tcgacgatcg gcagcgccag agccaagaac
     2041 acatgccagg cgtaggccac cgcgaccagc acgaggcaga tcgtgtggaa cgggtggccg
     2101 gcgaccgcct cccatgcagc cgtgatccat cgacgcggcc aggccgaatc gtcggcatcg
     2161 gcatcgccca ggaccctccg gtcaccctcg actccggaat cggacggatc cggtcgagca
     2221 gcctgcggat cggcgagcac gcgcgcgccg acatcgccgc gcgccgggaa cggcagccgc
     2281 tcgagaaggt cggcgaacag cccgatgccg tcgctggcga aggccagggc gagcgccgcg
     2341 ctcgccagcg ccaccaccgc ggtgacaccc agcagcacga acggctccaa ctcgtggatc
     2401 accgccccgg cgatgagcac ggacacgttg acctgcgcca gaccgatcac cgagccggcc
     2461 aggaccgcgg agaccctcga cgcgaacccg agtgacgaga cgaccagggc ggtcgagccg
     2521 acgacgagcg ccagcgccac gatcaacaac agcacttagc gactcctccg gtcgacgtct
     2581 gcgtaccgag ccacgatccg gtaggccacg acgaacgcaa cggtggccgc ggcgaggacg
     2641 atgccggaac cgacgacccg gtggctcacc tcgaagccgt cacggccgaa ctgacgggtc
     2701 gcgacggcga tgagcacgat gcgcccgagg ttgatcgtga acatcgtggc cgcggcggca
     2761 cccatcgcca ccacggcgcg cacgggcgag aaccgccgca ccatcgccat gatcccgccc
     2821 atggcgatca tcggaccgac cacgagcacc gaactgcacg acatcgtgac cttgagggca
     2881 cggagcccgt ggccggagta gttcgggtag aagacgaggt cggtgccggg cacgtgcacc
     2941 ccttcgccca acacgaactt caggggcggc gcggcgagcg ccgcctcggc cactcggacg
     3001 tcggcctgcg cctgcacgag caaggcccca gacgcgatga gcgcgacccc caagccggca
     3061 cggagcggcg agaacgagaa ggtgcgcggc gacgagggca gcggacggga cagcgcggtc
     3121 acggctgacc gaccgccgtc gtggcgctgg cggaggccat ggccccggcc ccggcctcac
     3181 cgccgacgga gacgggctgg cgcccctcgg cggccgcggc ggccgcaccc aggaagagcg
     3241 tcggcgacgc catctcctgc gcccggcggg tcttgtccca gccggtccgc ccgaccacca
     3301 tccggtacag cgctcgccag caggcggcat aggagatgta ggccgccagg atcaggaggt
     3361 ggccgagcgc cagcgacttg atcaatccgg tcgagcggac ccggcggaag tagagggagc
     3421 cgttgacgat gttcgggaag aagatcagca ggtaccagag caccatgccg accacctggg
     3481 ccacgaccga cgtgccgaag acctcccatc ccttccccga gatggtctgg taggtctgcc
     3541 agatgccgag gtggaacagg atcgaccagg ggagcacctg gagccacggc accgcgaggt
     3601 acgccgtgac ctcgagcacc gcaccgtggg gcagcgcccg tgaccgccag agctccggca
     3661 gccgccggcc gcaggtcatg tgcccctggt accagcgcgt ccgctgcttg atcaggggcc
     3721 ggatctgctc gaccccctgc tgggtcacgt gcgcctcgtc ggtggtggtg agcttccacc
     3781 cgttcgtcag catcgagatc gtgaggtcga ggtcctcggt gagcgactcg gaccacggca
     3841 cgccgtcgag gccgttgagc gcggtgagcc gggcgaactg gccgttgccg ccgaggctga
     3901 ccgtgccggt ggcgacgcgg ccgatctggc aggtggcggt caggccgaag aactcgatgt
     3961 cttggatcag cccgagcagc gtgtcgcggt tgcggatgcg cacgatcagc tgcgcgccgc
     4021 cgacgtcggg gtcgcggaac agcggcagca ccttgtcgag gcagccccgg gagaggtggc
     4081 cgtccgcgtc catcacgcag acgaggacgg tcgacggatc gatgtcctcc tcgtgcacga
     4141 tctcgttgat ccgggcgaac ccgtcgttga gggccttgcc cttgccgaga cgggcgttgg
     4201 gcagctcacg ccgcagcacc gtcacgcggt ctgcgtcgac cgcggcggcg atggcgctgg
     4261 tgcggtcatc cgagccgtcg tcgacgacca cgacacgagc ccggtcgtcg acgaggagcc
     4321 gctcgacggt ctcctcgatg acgagctcct cgttgagaca cccgatgagg aagaacacgg
     4381 tggtcggctc ggggccttcg ggcgtgaccg gttccttgcc gcgccgtcgc tcgaagagca
     4441 cccccgagga ggtcgtcatc aagaagtagg ccatgccgag acccacgatg atgagcgcca
     4501 gggcgccgat catgcctcga cctctcgatc atcgtcggcg atccgcagca cgtgggcgac
     4561 gatccgctcg gacgcgaagc cgtcgccgta gggactcggc aggtcggcca gctctgccag
     4621 gtgctcaggc ccagcttcga gcacctcggt gatcgcaccg aggagcgcgt cgggctgcac
     4681 gaggcgggcg aagcgctcca gcgactccgg gcgctccgtg gagttgcgca cgaccacgag
     4741 ggggcgcttc aacacggtgc actcctcctg cacgccgccc gagtccgaga ccaaaagcgc
     4801 ggcgtggttc gcgagggcga ggaacgctct cgatccgagg gggtccacca cgtggaggcc
     4861 atcggtcagc ggttggagcc cgtgccgctc gatggcgccg cgagtccgcg ggtgcagggg
     4921 gaggagcacc tggcggccgc aggcgccgag cgcccgcagg atgtcgccga gcttctccgg
     4981 gtcgtcggtg ttctccgggc ggtggatggt ggcgagcacg tagcccgcgt cgtcgcagcc
     5041 gtagcgctcg agcacgccac gccggtcggg cgccgcgggc agctggcgcg ccacggcctc
     5101 cacgacggtg ttgccggtga tgaccgcacg atcggcgagg ccctcgaggg cgaggttgtc
     5161 ggcgttgagc ttggtggccg cgcacagcac gtcggagacg tgatcggtga ggacgcggtt
     5221 gtgctcctcg ggcatggccg agtcgtagct gcggaggccg gcctctacgt ggaacagcgg
     5281 gatggaggcc gagttggcgg ccagcgcacc cgccaaggtg gcgttggtgt ctccctgcac
     5341 gatgaccaca tcaggccggt gccggtcgaa cagcccgtcg aggtcagcca gcgcctggcc
     5401 gacctggccg ccccgacgca ggccgccgac cccgagctga acatcggggt cggcgaggcc
     5461 gtgctcttcg aagaaggagc tcgacagtga cgcgtcgtag tgctggccgg tgtggacaac
     5521 cgtggcgcgg tcaccgagga gctggatgat ccgggcgagc ttgatgatct ccggacgggt
     5581 ccccaagaca acggcgaccg aacgtcggct ttcaccaaca agaccagatg acatcagcag
     5641 tgaccccccc tttgatcagt gtgtgagctg gtcaggtcgc cggagcggcc gactcgcgct
     5701 cggtccgggc gaggaccgcg atccgcagga gcacgaggcc accgacgatg agcagagccg
     5761 cgatgatgcc gaggctcagc gtgttgaaac cggtcacggc aagcgtgccg ccgccgatgc
     5821 cgatcacgcc agccactgcg gcgccaggcg ggggagagtt atacatctgt tctgctcctt
     5881 gtgggtcagg tctccgctgt cggccgaccc ttgtcctctg gttgaacaac ccgaggtggg
     5941 gggtcctatg acggtgagac ggtggagccc gtccgtgacc ggttccctgg gtcgccgagg
     6001 cggcctgggg ctccgatcgt caacgcgctg gcgcaggctc cggggcggtc tggcggcggg
     6061 agcgccacca gaccagcagc ccgccgccgg aggcgacggt cactgcggcg aataggtacg
     6121 ggtcgaccac cgggatgggg tcctctgccg ggaggtggag ggcgacgtcg gtcgcagacc
     6181 cccaggcgat gctgcgagat ccgttcttca gctcctgggt cacctggccg agcgtccacg
     6241 tgggggtgag ctccaccgca ccaccggtga ccacgatctg gtcgaagctg agggtgaacc
     6301 cggcgtagta gccgttgttg gagagcacgt tgacgcaccg gtcgtaggcc atctggtccc
     6361 agaacgccgc gagcgtggcc ccgttgaccg atgcgctcat ctcggtggag ccgacggacg
     6421 gtccgccgac ggtcaccccc gtcggcgggt cggtccgcgc gccctcctgg ccgttctgcg
     6481 ccaggcagtt gatggccggg ccgttgccgc ggtcgccgtt gtcggtccgg ctcatggtga
     6541 cgttggtcgg tgtcgagaga ctcgtcccgg tctcggccgg gagcccgacc cggatcgcga
     6601 accgctcgtc gcggctgttg ttccggatcc cgtcggaggt ctcctggtag taggcgtcgt
     6661 ggccgcccct cgggggaacg gtcacgcgca cggtcaccac atcgccgacc acaagtgagg
     6721 tccccggcgc cacgtcgacg ccgttcacca ggatctgggt gacgatgctc aggttctggc
     6781 acgtggagac cgggccctgg ttctggtgca acccgttcag gttgcagttc acgctcgcgc
     6841 ccacgtcgct ctgggtccgg gtcgctgctc ctgccgtagc cggcaggag
//
LOCUS       DATMFS010000569         5443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_135913, whole genome shotgun sequence.
ACCESSION   DATMFS010000569 DATMFS010000000
VERSION     DATMFS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135913"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VNQ33_10810"
     CDS             <1..726
                     /locus_tag="VNQ33_10810"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWJ98641.1"
                     /translation="DRPPFQAWTDEDGTLHETWRISDPAQIEAIAGAVGSHPLVIADG
                     HHRYETSLAYRDERRAADGDGGDADATLCYVVELSDEQLTVRPIHRLVAGLADRDLAD
                     ALAGLPGSARVGTAAADEVADGRVLLQMRDQGAIAVVGPDGSAVLVRLDPASYPDVDD
                     LDSARLAAALTALGPHELAYQHGTDRAQAAVASGAADWAVLIRPVTVAAIEDNAHSGK
                     RMPPKSTFFYPKPRTGIVFRTLG"
     gene            complement(730..1476)
                     /gene="lepB"
                     /locus_tag="VNQ33_10815"
     CDS             complement(730..1476)
                     /gene="lepB"
                     /locus_tag="VNQ33_10815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWJ98642.1"
                     /translation="MTDQPPAGPPTSGDPAPDDGFARRQQREEAVVGEAGSASDGSDR
                     SDDAEAAEAETRAQRRKPKSQTRNMLEWVAVIGGAILIAVVVRTFILQTFWIPSPSMS
                     PTLVENDRVLVNKLAYRFHDVNRGDVVVFERPENEPPSEIKDLIKRVVALPGERVSIM
                     DGKVKINGRALDEPYTHNLQTVLDSCPITYVPGIDTDQGFKVPEDHVLVLGDNRTNSH
                     DGRCFGAIDEDLIVGRAFFLMWPPGHAGGL"
     gene            complement(1488..2474)
                     /locus_tag="VNQ33_10820"
     CDS             complement(1488..2474)
                     /locus_tag="VNQ33_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWJ98643.1"
                     /translation="MRAPRITATGRLQRLLAILQWAARQPDGALVDDLCERFGLSQAD
                     LVKELDLANMINADSPFYDEMPFEVFLEEDRVFVRLFSFRRPMRLTPAEGLALVAAAD
                     SLVDDEAEGASSPLGRALAKLAGLLGIEPGQAVDVDLDPDGGETGRRLRQAIAEDRRV
                     SFVYWTYGRDAVARREVDPWALFSEGATWYLAAWAHDAEASRNFRLDRMQDLAVEDAP
                     RREPAPASLDRSARTVDEAPRVVLDLPPWAWWVAEAHPIISAEQRDDQHLRVTLAVAG
                     SSWLERLLLRLGPGATVVEIDHELGGPDLAARAAARVLARYQPAESGARAAR"
     gene            complement(2471..3415)
                     /locus_tag="VNQ33_10825"
     CDS             complement(2471..3415)
                     /locus_tag="VNQ33_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWJ98644.1"
                     /translation="MALVEKVEKIERLLSLMNALLGAPRAISADDLRSRVPGYPDEDA
                     SFKRAFERDKDDLREMGVPLLVETIGGSDPPLLGYRIRTQDYELRDPGLEPDELEALN
                     LAAAATGLAGGAGQRALFKLGGAAPSAAPQAALPVDPDLVAAFTGVAERRVIAFRYHD
                     TDREVHPYRLEFVRGRWYLNGFDRVRGDDRWFRMGRIQSAIEVTDPAGAFERPEEAVP
                     GLQLDPWALGGQSDPVSALVWFDPEVAHSVRTELPPGALRSDDRDGLVAELTVTNRDG
                     FRSWLLTFLDRAEVLEPQELRDDMVAWLTEVIAGEERA"
     gene            complement(3418..4461)
                     /locus_tag="VNQ33_10830"
     CDS             complement(3418..4461)
                     /locus_tag="VNQ33_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HWJ98645.1"
                     /translation="MPSFRTATVTEVLVERDGFVRVRLDDGSRAFALTQLTGPIGPGD
                     EVAVNTTAVELGLGSGGWHIVHWNLTRGGWSEPGPGHLMKLRYTSLQVDAGAAEEDGA
                     VPRSLHGCPVIVAGVHSQLPVAAAAIGALRPGTRTAYVMTDGAALPLAMSDQVVRLRD
                     TGLLCGTVSAGHAFGGDLEALNVPSALALARHRLDAEVIVVAMGPGIAGTGSELGFTG
                     LEAAPALDAAGWLEGRPVYCLRCSDADPRARHRGISHHSRTVLDAVRSEVDVALPPGV
                     DASDDRHRWHPIHPGDVGRLLADNGLEVTTMGRGPQEDPLYFAAAAASGHLAVRLLSG
                     GGDPMLAASPSSS"
     gene            complement(4470..>5443)
                     /locus_tag="VNQ33_10835"
     CDS             complement(4470..>5443)
                     /locus_tag="VNQ33_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWJ98646.1"
                     /translation="RRDDFGHYAEVLIPFLVSRQIYAGAGKVLQTARGAMFSLSQRAE
                     HIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSEHATFLKVGATSLLL
                     RMLEEPNVVLRDMTLENPIRAIREISHDITCTRRVRLANGREVSALDIQGEYLQRAIR
                     YAETRGLSPEEKVALDAWEHAVTSIERDPLSLDREADWVMKYKMIEGYRERHDLALSD
                     PKVALIDLQYHDINRDRGLFYRLQDRGLAERVCDDEEIDIATDEAPQTTRARLRGEFI
                     RKAKERKRDYTVDWVHLKLNDQAQRTVLCKDPFKAHDERVEKLIASL"
BASE COUNT          733 a         1917 c         2016 g          777 t
ORIGIN      
        1 gaccgcccgc ccttccaggc ctggaccgac gaggacggca cgctccacga gacgtggcgc
       61 atcagcgatc cggcccagat cgaggccatc gccggagccg tcggctccca tccgctggtc
      121 atcgccgacg gccaccaccg ctacgagacg agcctggcct accgcgacga gcgccgtgca
      181 gccgatggcg acggcggcga tgccgacgcc accctctgct acgtcgtgga gctgtccgac
      241 gagcagctga cggtccggcc gatccaccgc ctcgtcgctg ggctggccga ccgggacctg
      301 gccgacgccc tcgccggcct gcccgggtcg gcccgggtcg gcaccgccgc ggccgacgag
      361 gtcgccgacg gccgggtcct gctccagatg cgggaccagg gcgccatcgc cgtcgtcggc
      421 cccgacggct cggcggtcct cgtccgcctg gatccggcgt cgtaccccga cgtcgacgac
      481 ctcgacagcg cccgcctcgc cgcggccctc acggcgctcg gcccgcacga gctcgcctac
      541 cagcacggca cggaccgagc gcaggccgcc gtcgcctccg gggccgcgga ctgggcggtg
      601 ctgatccgac cggtcacggt cgcggccatc gaggacaacg cccactcggg caagcggatg
      661 cccccgaaga gcacgttctt ctacccgaag ccccggacgg ggatcgtgtt ccgcaccctc
      721 ggctgagcgt cagaggccgc cggcgtggcc cggcggccac atcaagaaga aggcccggcc
      781 cacgatgagg tcttcgtcga tggcgccgaa gcagcggccg tcgtgcgagt tcgtccggtt
      841 gtcgccgagc acgagcacgt ggtcctcggg caccttgaac ccctggtcgg tgtcgatgcc
      901 cggcacgtac gtgatgggac acgagtcgag caccgtctgc aggttgtgcg tgtagggctc
      961 gtcgagggcg cggccgttga tcttgacctt gccgtccatg atcgacaccc gctcgccggg
     1021 cagggccacg acccgcttga tgaggtcttt gatctcgctc ggaggctcgt tctcggggcg
     1081 ttcgaacacc acgacgtcgc cccggttcac gtcgtggaac cggtaggcga gcttgttgac
     1141 cagcacgcgg tcgttctcga ccagcgtcgg cgacatcgac ggcgacggga tccagaacgt
     1201 ctgcaggatg aaggtgcgga cgaccaccgc gatgaggatc gccccgccga tcaccgcgac
     1261 ccactcgagc atgttgcggg tctgggactt gggcttcctg cgctgcgccc gggtctccgc
     1321 ctcggcggcc tcggcgtcgt cggagcggtc ggacccgtca gatgccgagc cggcttcgcc
     1381 caccacggcc tcctcacgct gctggcgtcg ggcgaagccg tcgtcgggag cgggatcgcc
     1441 cgacgtcggc ggtcctgcgg ggggctgatc ggtcacaagg agcgaaccta gcgcgccgcc
     1501 ctcgcgccgc tttccgcggg ctggtaccgg gcgaggaccc gagccgcggc gcgagcggcc
     1561 aggtccggcc cgccgagctc gtggtcgatc tccacgaccg tcgcccctgg tccgagccgc
     1621 agcaggagcc gctcgagcca cgaggatccg gcgacggcga gggtcacgcg caggtgctgg
     1681 tcgtcgcgct gttcggcgga gatgatcggg tgcgcctcgg ccacccacca ggcccagggg
     1741 gggaggtcga ggacgacgcg cggcgcctcg tcgacggtcc gggcgctccg gtcgagcgat
     1801 gctggcgctg gctcgcggcg cggcgcgtcc tcgacggcga ggtcctgcat gcggtcgagc
     1861 cggaagttcc ggctcgcttc ggcgtcgtgg gcccacgccg ccaggtacca cgtcgctccc
     1921 tcgctgaaga gcgcccacgg atcgacctca cgacgagcga cggcgtcgcg gccgtacgtc
     1981 cagtacacga acgagacacg ccggtcctcg gcgatggcct ggcgcaggcg gcgcccggtc
     2041 tccccaccgt cggggtcgag gtcgacgtcg accgcctgac ccggttcgat gccgaggagg
     2101 ccggcgagct tggccagggc gcggccgagg gggctggacg cgccctcggc ctcgtcatcg
     2161 acgagcgagt cggccgcagc caccagggcg agcccttcgg ccggcgtgag ccgcatcggc
     2221 cgccggaacg agaacaggcg cacgaacacg cggtcctctt cgaggaacac ctcgaagggc
     2281 atctcgtcgt agaacggcga atcggcgttg atcatgttgg cgaggtcgag ctccttcacg
     2341 agatcggcct ggctcaggcc gaagcgctcg cagaggtcgt ccacgagggc gccgtcgggc
     2401 tgccgcgccg cccactgcag gatcgccagc aggcgctgga gccggccggt cgcggtgatc
     2461 cgcggcgccc tcacgcccgc tcctcccccg cgatgacctc ggtcagccac gccaccatgt
     2521 cgtctcgcag ctcctggggc tcgagcacct cggcccggtc gaggaacgtc aggagccacg
     2581 agcggaagcc gtcgcggttg gtgacggtga gctcggccac gagcccgtcg cgatcgtcgg
     2641 agcggagggc gccgggcggg agctcggtgc ggacggagtg ggcgacctcc gggtcgaacc
     2701 agaccagggc ggagacggga tcggattggc cgccgagcgc ccacgggtcg agttggaggc
     2761 cgggcacggc ctcctccggg cgctcgaacg cgccggccgg atcggtgact tcgatcgcgc
     2821 tctggatccg gcccatgcgg aaccagcggt cgtcgccccg cacgcggtcg aacccgttga
     2881 ggtaccagcg gccccgcacg aactcgaggc ggtacgggtg gacctctcgg tcggtgtcgt
     2941 ggtagcggaa ggcgatgacg cggcgctcgg ccacccccgt gaaggcggcg accaggtccg
     3001 ggtcgaccgg gagcgcggcc tggggcgcgg ccgacggtgc ggcgccgccg agcttgaaga
     3061 gcgctcgctg cccggctccg ccggcgagcc cggtggcggc cgccgccagg ttcagggcct
     3121 cgagctcgtc gggttcgagg cccgggtcgc gcagctcgta gtcctgggtc ctgatgcggt
     3181 agccgaggag cggcggatcg gacccgccga tggtctccac gaggagcggg acccccatct
     3241 cccggaggtc gtccttgtca cgctcgaagg cccgcttgaa cgaagcgtcc tcgtcggggt
     3301 agccggggac ccggctccgc aggtcgtcgg cgctgatcgc ccggggtgca ccgaggaggg
     3361 cgttcatcag gctgaggagc cgctcgatct tctccacctt ctccacgagc gccatgccta
     3421 gctgctcgac ggtgaggcgg cgagcatcgg gtcgccgccg cctgacagga gtcgcaccgc
     3481 gaggtgccct gacgcggcgg cggccgcgaa gtagagcgga tcctcctgcg ggcctcgccc
     3541 catggtcgtg acctcgagac cgttgtcggc caggaggcgg ccgacgtcac cggggtggat
     3601 cgggtgccag cggtgccgat catcggaagc gtcgacgccg ggcggcaggg ccacgtcgac
     3661 ctcggagcgc acggcgtcga gcacggtgcg gctgtggtgg ctgatcccgc ggtggcgagc
     3721 ccgtggatcg gcgtcggagc accgcaggca gtacaccggc cggccctcca gccatcccgc
     3781 cgcgtccagc gccggagcgg cctcgaggcc ggtgaacccg agctcgctgc ccgtgccggc
     3841 gatgccgggc cccatggcca cgacgatcac ctccgcgtcg aggcggtggc gggcgagggc
     3901 cagggcggac gggacgttca gggcctcgag gtcgccgccg aacgcatgcc cggccgacac
     3961 cgtgccgcag aggagcccgg tgtcgcgcag ccgcaccacc tgatcgctca tggccagcgg
     4021 gagcgcggcg ccgtcggtca tcacgtaggc ggtgcgcgtg ccgggccgca gggcaccgat
     4081 cgccgccgcc gcgacgggca gctggctgtg gacgcccgcg acgatcaccg ggcagccgtg
     4141 gagcgagcgc gggaccgccc cgtcctcttc ggctgcgccc gcgtcgacct ggaggctcgt
     4201 gtagcggagc ttcatgaggt ggccgggacc aggctcggac catccgccgc gggtgaggtt
     4261 ccagtgcacg atgtgccagc cgccgctgcc gagccccagc tcgacggcgg tggtgttgac
     4321 cgcgacctcg tcgccggggc cgatcgggcc ggtcagctgg gtgagggcga aggcgcgact
     4381 gccatcgtcg agacggacgc ggacgaaccc gtctcgctcg acgaggacct cggtgacggt
     4441 cgcggtgcgg aaggaaggca agccgggccc tacagggagg cgatcagctt ctcgacccgc
     4501 tcgtcgtggg ccttgaacgg gtctttgcac agcaccgtgc gctgggcctg gtcgttgagc
     4561 ttcaggtgca cccagtccac cgtgtagtcg cgcttgcgct ccttcgcctt gcggatgaac
     4621 tcgccgcgga gccgggcccg tgtggtctgc ggggcctcgt cggtggcgat gtcgatctcc
     4681 tcgtcatcgc agacccgctc ggccaagccg cgatcctgga ggcggtagaa gagcccccgg
     4741 tcgcggttga tgtcgtggta ctgcaggtcg atcagggcga ccttggggtc cgacagcgcg
     4801 aggtcgtggc gctcccggta gccctcgatc atcttgtact tcatgaccca gtcggcctct
     4861 cggtcgagcg acagcgggtc ccgctcgatg ctggtgacgg cgtgctccca cgcgtcgagc
     4921 gccaccttct cctcggggct gagcccccgg gtctcggcgt accggatggc ccgctgcagg
     4981 tactcgcctt ggatgtcgag cgccgagacc tcccggccgt tggccaagcg gacccgccgc
     5041 gtgcaggtga tgtcgtggct gatctcgcgg atggcgcgga tcgggttctc gagggtcatg
     5101 tcccggagca ccacgttggg ctcctcgagc atccgcagca gcagcgaggt ggcgcccacc
     5161 ttgaggaagg tggcgtgctc gctcatgttc gagtcgccca cgatcacgtg gaggcgccgg
     5221 tacttctcgg cgtcggcgtg aggctcgtcg cgcgtgttga tgatgggcct gctgcgggtg
     5281 gtggcgctgg agacgccctc ccagatgtgc tcggcgcgct ggctgagcga gaacatcgcc
     5341 ccgcgggcgg tctggaggac cttcccggcc ccggcgtaga tctggcggct gacgaggaac
     5401 gggatgagga cctcggcgta gtggccgaag tcgtcacggc ggc
//
LOCUS       DATMFS010000570         2966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_114279, whole genome shotgun sequence.
ACCESSION   DATMFS010000570 DATMFS010000000
VERSION     DATMFS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114279"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VNQ33_10840"
     CDS             <1..766
                     /locus_tag="VNQ33_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ98647.1"
                     /translation="LAEAADRLGTGRFDIDPPHYSIPEAEAIGDALRSSSERLGELVR
                     REREFAANASHQLRTPITALRLELEDLALWPQTPPEVADELNRYLPELDRLSSAIDEL
                     LGLARGLRLGDAVDLDLNELVADVVERWQPQVEALGHDVNHDDRGPVPARVVPGPVVQ
                     ILDVLIENAASHGVGPITVSARDAGKYVEIVVGDSGRRTFGNEIFSRGTSRKEGDAGG
                     LGLTIAADLATTMGGYLKLTEDELTTFELMLPGRGA"
     gene            complement(790..1257)
                     /locus_tag="VNQ33_10845"
     CDS             complement(790..1257)
                     /locus_tag="VNQ33_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWJ98648.1"
                     /translation="MTTVLTYGTFDLFHIGHLRLLERLSSMGDRLVVGVSTDDFNAGK
                     GKSTVVPYADRAAIVGAIKGVDQVLPEESWEQKRSDIVEHGVDLFVMGDDWTGKFDDL
                     SDLCRVEYLPRTKGISTTGLKEMLRVLDPGHIEEMQDALGVLSRLLDHYRDLT"
     gene            1333..1746
                     /locus_tag="VNQ33_10850"
     CDS             1333..1746
                     /locus_tag="VNQ33_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWJ98649.1"
                     /translation="MTSWQDPDAVSLMLDDLETWAVVGLSTNTSRTAYSIAQLLQRRG
                     KRIVPIHPDAPTVLGAQGYATLADVPFPIDVVDVFRRSEDAGPFADQAVEVGAKAVWF
                     QLGVIDEAAFERTIAAGVPMVMDTCPAIEWNRRAR"
     gene            complement(1748..2854)
                     /locus_tag="VNQ33_10855"
     CDS             complement(1748..2854)
                     /locus_tag="VNQ33_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWJ98650.1"
                     /translation="MTDAPDATRRPGSRASNRRSFRQRHPMVVWSVVLIVLLPLVGGA
                     AYAFNLKRKLDDVETVKLNPQTEPDPDEGRALNILLLGSDKGEPQEGQSKNTTISEDA
                     ASGDWPNGKYRSDTLMVVHIPADRKKVYLVSLPRDSFVPIYDENGKTTHSEKINAAFS
                     AGGPLGAINTVENLTGLKMDHLAIIDWEGFKDLSSAVGGVEVTIPQSFYDPKQKVQWE
                     AGKQTLEGKKALAYVRTRYGLVGGDFDRIKRQQNFMRSLMGKMLSSGVTTNPNKLTRT
                     VTALTENLTVDEGWSGTAMAKLAVSLRGVRTDDVTFMTAPVQGTDTDPTYGSIVVLQQ
                     DKMSELFKALSRDRMAEYLQKYPDDVLPDPEDVS"
BASE COUNT          425 a         1009 c         1045 g          487 t
ORIGIN      
        1 gctggccgag gccgccgacc ggctcggcac gggacgcttc gacatcgatc cgccgcacta
       61 ctcgatcccg gaggccgagg cgatcggcga cgccctgcgc agcagctcgg agcggctcgg
      121 cgagctggtc cggcgcgagc gcgagttcgc cgcgaacgcc tcgcaccagc tgcgcacccc
      181 gatcaccgcg ctgcggctcg agctcgagga cctcgcgctg tggccccaga cgccgcccga
      241 ggtggctgac gagctcaacc gctacctgcc cgagctggac cgcctcagca gcgcgatcga
      301 cgagctgctg ggtctggcgc gggggctccg cctcggtgac gccgtcgacc tcgacctcaa
      361 cgagctggtg gccgacgtgg tcgagcgctg gcagccccag gtggaggcgc tcggccacga
      421 cgtcaaccac gacgaccggg gcccggtgcc ggcgcgggtc gtccccggcc cggtcgtgca
      481 gatcctcgac gtcctcatcg agaacgccgc gagccacggc gtcggaccga tcacggtgtc
      541 ggcccgcgac gcggggaagt acgtcgagat cgtggtcggc gacagcggcc gtcgcacgtt
      601 cggcaacgag atcttcagcc gcggcacgag ccgcaaggag ggcgacgccg gcggactcgg
      661 tctgacgatc gcggccgacc tcgccaccac gatgggcggc tacctcaagc tgaccgagga
      721 cgagctcacg accttcgagc tcatgctgcc cggtcgcggc gcctgaccgc tcgggtcagg
      781 tcgcgccact caggtcaggt cgcggtagtg gtcgagcagg cgtgagagga cgcccagcgc
      841 gtcctgcatc tcctcgatgt gcccggggtc gagcacccgc agcatctcct tgagtcccgt
      901 cgtggagatg cccttcgtgc ggggcaggta ctccacgcgg cacaggtcgg acaggtcgtc
      961 gaacttgccg gtccagtcgt cgcccatcac gaacaggtcg acgccgtgct cgacgatgtc
     1021 gctgcgcttc tgctcccacg actcctcggg cagcacctgg tcgacgccct tgatcgcgcc
     1081 gacgatcgca gcccggtcgg cgtagggcac cacggtgctc ttgcccttcc cggcgttgaa
     1141 gtcgtcggtg gagaccccca cgacgagccg gtcgcccatc gaggagaggc gctccagcag
     1201 gcgcaggtgg ccgatgtgga agaggtcgaa ggttccgtac gtcaggacgg tcgtcacgcg
     1261 atcaggatac ggaccgggtg cccgcgtggc cgccgaccgg cgggcgtggg tgtcggcggc
     1321 tacggtcgga ccatgacctc gtggcaggat cccgacgccg tctccctcat gctcgacgac
     1381 ctcgagacgt gggccgtggt gggcttgtcg accaacacct cgcgcaccgc gtactcgatc
     1441 gcgcagctgc tgcagcggcg aggcaagcgc atcgtgccga tccatcccga cgcgcccacg
     1501 gtgctggggg cgcagggcta cgccacgctc gccgacgtgc cgttcccgat cgacgtggtc
     1561 gacgtgttcc gccgctccga ggatgccggg ccgttcgccg accaggccgt cgaggtcggc
     1621 gccaaggccg tgtggttcca gctgggcgtc atcgacgagg cggcgttcga gcgcacgatc
     1681 gcggcgggcg tgccgatggt gatggacacc tgcccggcga tcgagtggaa ccgccgagcc
     1741 cgctagctca gctgacgtcc tcgggatcgg gcaggacgtc gtcggggtac ttctggaggt
     1801 actcggccat ccggtcgcgg ctgagggcct tgaacagctc gctcatcttg tcctgctgca
     1861 gcacgacgat gctgccgtag gtgggatcgg tgtcggtgcc ctgcaccgga gccgtcatga
     1921 acgtgacgtc gtcggtgcgg acgcctcgca gcgacaccgc cagcttggcc atcgcggtgc
     1981 ccgaccagcc ctcgtccacc gtgaggttct cggtcagggc cgtgaccgtg cgggtgagct
     2041 tgttcgggtt ggtggtcacg cccgagctga gcatcttgcc catgagcgag cgcatgaagt
     2101 tctgctgacg cttgatgcgg tcgaagtcgc cgccgacgag gccgtagcgg gtgcgcacgt
     2161 acgccagggc cttcttgccc tcgagcgtct gcttgccggc ctcccactgc accttctgct
     2221 tggggtcgta gaaggactgg gggatcgtca cctccacgcc gccgaccgcg gacgagaggt
     2281 ccttgaagcc ctcccagtcg atgatcgcca agtggtccat cttcaggccg gtgaggttct
     2341 ccaccgtgtt gatcgcgccg agcgggccgc cggcgctgaa cgcggcgttg atcttctccg
     2401 agtgggtggt cttgccgttc tcgtcgtaga tcggcacgaa cgagtcgcgc ggcagcgaga
     2461 cgaggtagac cttcttccgg tcggccggga tgtgcacgac catgagcgtg tcgctgcggt
     2521 acttgccgtt gggccagtcg cccgacgcgg cgtcctcgga gatcgtggtg ttcttcgact
     2581 gcccctcctg cggctcgccc ttgtccgagc ccagcagcag gatgttgagc gcgcggccct
     2641 cgtcggggtc gggctcggtc tgggggttga gcttcacggt ctcgacgtcg tcgagcttgc
     2701 gcttgaggtt gaaggcgtag gcggcgccgc cgacgagcgg cagcagcacg atgagcacga
     2761 ccgaccagac gaccatcggg tggcgctggc ggaaggaacg acgattcgac gctcgcgatc
     2821 cgggtcgccg ggtcgcatcg ggggcgtcgg tcaccgcatc ctcctcatcg tcgtcgtatc
     2881 aaccttccca gtatgacggg ggttgctgag aggggccctc aggaatccag cacgatcgtc
     2941 acgggcccgt cgttgaccag ctcgac
//
LOCUS       DATMFS010000571         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_179743, whole genome shotgun sequence.
ACCESSION   DATMFS010000571 DATMFS010000000
VERSION     DATMFS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179743"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VNQ33_10860"
     CDS             <1..438
                     /locus_tag="VNQ33_10860"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HWJ98651.1"
                     /translation="GSWTMPCDEAYLEAIRLELDDLVGDLRSQGAPVVLLTAPGTSLS
                     WVLERVPPGMPERVACTNQVLDDLADRTPGVTIIDLASYICPPGEACKTEIDGVDLRE
                     DGLHFTGAGARAVNNWLIPQVTAIAQAARQQDPQHGPTTTAGG"
     gene            complement(442..1329)
                     /locus_tag="VNQ33_10865"
     CDS             complement(442..1329)
                     /locus_tag="VNQ33_10865"
                     /inference="COORDINATES: protein motif:HMM:NF021850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative capsular polysaccharide synthesis
                     family protein"
                     /protein_id="HWJ98652.1"
                     /translation="MGYRIQGERVRRGARVLRRHWSQAGLRALEALDRPPVVVYSWSK
                     TGSTTAHETLARSRLWRPVYHVHSLDDGVEERQAKHASTGNSLGLSPEELRVVRRLRA
                     QVQAGDTPMAVVTLVRDPISRSISSFFQRHYGSGIDMTAEHPSAADIAATIEQLHERL
                     PKFILATDVWFDRQLRGGLGVDLFAEPFDPEVGFARVRHGAFDQAVIRTDRFDELYRP
                     ALEPILGRRLGPVRRVNDASSKRYRAHHRATLEVFRLSDDEIDQALATRVIGHFFSPA
                     ERDALARSWRGGAPAAGSR"
     gene            complement(1387..>2143)
                     /locus_tag="VNQ33_10870"
     CDS             complement(1387..>2143)
                     /locus_tag="VNQ33_10870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98653.1"
                     /translation="AVLTWGNDARNPLYRIPPYRQALKRSMGADLVVCLIEAARDHCL
                     ELGYRPEQLAVVLPPLDTERFHPPVAPVEAPIAAFISPLAANKGIDRVLDAWPLVTAR
                     VPEAKLVVAGRGPLEGLVRERAATDPSVEFLGALPGDGVAELLRTVAVFVTAPRPTRV
                     WNEQFGLAYVEAMASGVPVVTTECGTNHEAVLAPSLRVPDAVGPVADGLVHFLGDPGL
                     RRSISGELRDVVVERFERGQQLAALRAAFDSAP"
BASE COUNT          307 a          757 c          820 g          259 t
ORIGIN      
        1 ggctcgtgga cgatgccgtg cgacgaggcc tacctggagg cgatccggct cgagctggac
       61 gacctggtgg gcgacctgcg gtcgcagggt gcgccagtgg tcctgctcac ggcaccggga
      121 acgtcgctca gctgggtgct cgagcgggtg ccacccggga tgcccgagcg ggtggcgtgc
      181 accaaccagg tgctcgacga cctggccgat cgcaccccgg gcgtgaccat catcgacctg
      241 gcctcctaca tctgcccgcc gggcgaggcg tgcaagaccg agatcgacgg cgtcgatctg
      301 cgcgaggacg gcctgcactt cacgggagcc ggggcccggg ccgtgaacaa ctggctgatc
      361 ccccaggtga ccgccatcgc tcaagccgcc cgccagcagg acccgcagca cggaccgacc
      421 accaccgcgg gcggttagcg gtcagcggct ccccgccgcg ggtgcgccgc cgcgccacga
      481 ccgggccagc gcgtcgcgct cggctgggct gaagaagtgc ccgatcaccc gcgtggcgag
      541 cgcctggtcg atctcgtcgt ccgacagccg gaacacctcg agggtggcgc ggtggtgcgc
      601 gcggtaccgc ttcgagctgg cgtcgttgac ccgccggacg gggcccagcc ggcggccgag
      661 gatcggctcg agcgccggcc ggtagagctc gtcgaaccgg tcggtgcgga tgaccgcctg
      721 gtcgaacgcc ccgtggcgga cgcgggcgaa gccgacctcg gggtcgaacg gctcggcgaa
      781 gaggtcgacc ccgagtccac cgcgcagctg gcggtcgaac cagacgtcgg tcgcgaggat
      841 gaacttcggg agccgctcgt ggagctgctc gatcgtggcg gcgatgtcgg ccgccgacgg
      901 gtgctccgcc gtcatgtcga tgccggagcc gtagtgccgc tggaagaagc tcgagatcga
      961 gcgggagatc ggatcccgca cgagggtcac gacggccatc ggcgtgtcgc ccgcctgcac
     1021 ctgggcacgc agtcgccgga cgacgcggag ctcctcgggg gagagcccga ggctgttgcc
     1081 ggtgctggcg tgcttcgcct ggcgctcctc gacgccgtca tcgagcgagt gcacgtggta
     1141 cacgggccgc cagaggcggg agcgagccag cgtctcgtgg gcggtggtgc tgccggtctt
     1201 cgaccacgag tacacgacca cgggaggccg gtcgagcgcc tccagcgctc ggagcccggc
     1261 ctgcgaccag tgccgccgga ggacgcgagc gccgcgccgg acccgttcgc cctggatccg
     1321 ataacccacg gtttcggaac ctagccgacc gtgaggcgcc cggcccggcc caggagcggt
     1381 cgcccgtcag ggagccgagt cgaacgcggc acgcagcgcg gccagctgct ggccccgctc
     1441 gaagcgctcg accacgacgt cgcggagctc gccggagatc gagcgccgga gcccgggatc
     1501 gccgaggaag tgcacgagcc cgtcggcgac gggccccacg gcatcgggga cccggaggct
     1561 cggggcgagc acggcctcgt ggttcgtgcc gcactcggtg gtgaccaccg ggacgccgct
     1621 cgccatggcc tcgacgtagg ccaggccgaa ctgctcgttc cagacccggg tgggccgcgg
     1681 ggcggtgacg aacacggcga ccgtgcgcag cagctcggcg acaccgtcgc cggggagcgc
     1741 gccgaggaac tcgaccgacg gatcggtggc ggcccgctcg cgcaccagcc cctccaacgg
     1801 ccctcggccc gcgaccacga gcttggcctc gggcacccgg gcggtgacca gcggccacgc
     1861 gtcgaggacc cggtcgatgc ccttgttggc ggcgagcggc gagatgaacg cggcgatggg
     1921 cgcctcgacc ggcgcgaccg gcggatggaa gcgctccgtg tcgagcggcg gcagcaccac
     1981 ggcgagctgc tcgggccggt agccgagctc caggcagtgg tcccgggccg cctcgatgag
     2041 gcagaccacg aggtccgcgc ccatcgagcg cttcagggcc tggcggtacg gcgggatccg
     2101 gtagaggggg ttccgggcgt cgttgcccca ggtgaggacc gcc
//
LOCUS       DATMFS010000572         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_159335, whole genome shotgun sequence.
ACCESSION   DATMFS010000572 DATMFS010000000
VERSION     DATMFS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159335"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VNQ33_10875"
     CDS             complement(<1..535)
                     /locus_tag="VNQ33_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98654.1"
                     /translation="MPPTSRTRNRAAALALVAAVLASSCSLLPGRDDDRGGPNDDVTV
                     TEVESGLAAAGKPERGGQLVYGLEAEVGETGYCMPEAQLAISGMQVARALYDTLTVPD
                     EDGDYVPYLAKAIDHDDAYKEWTITLRPGVTFHDGSTLDAKVVKNNLDAYRGAYANRS
                     PLLFTFVLANIAKVEALN"
     gene            complement(1370..2131)
                     /locus_tag="VNQ33_10880"
     CDS             complement(1370..2131)
                     /locus_tag="VNQ33_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWJ98655.1"
                     /translation="MPSSPWPFLDHPGPLAFAHRGGAGDWPENTMPAFEGAVELGYRY
                     VETDAHVTADGICLAFHDDRLDRVTDRTGVIAELTYDEVRRARVDGREPIPLLEDLIA
                     AFPDLRVNIDPKHDAAVEPLASVIERTESVDRVCVGSFSDARINRLRSALGPRLCTSL
                     GPKAIARLRGGSFGLPAGRITGGCAQVPHKYKGVAVTDRRFVERAHAAGLQVHVWTID
                     DAEEMHLLLDLGVDGIMTDRPAVLREVLRSRGAWY"
BASE COUNT          291 a          763 c          819 g          329 t
ORIGIN      
        1 cgttgagcgc ctcgaccttg gcgatgttgg cgaggacgaa ggtgaagagc agggggctgc
       61 ggttggcgta ggcaccgcgg taggcgtcga ggttgttctt cacgaccttc gcgtcgagcg
      121 tcgagccgtc gtggaaggtg acgcccgggc gcagcgtgat cgtccactcc ttgtacgcgt
      181 cgtcgtggtc gatcgccttg gccaggtacg gaacgtagtc gccgtcctcg tcgggcaccg
      241 tgagggtgtc gtagagggct cgggcgacct gcatcccgga gatggcgagc tgggcttcgg
      301 gcatgcagta gccggtctcg ccgacttcgg cctcgaggcc gtagaccagc tggccgccgc
      361 gctcgggctt gcccgcggcc gcgaggccgc tttcgacctc ggtcacggtc acgtcgtcgt
      421 tgggaccccc gcggtcgtcg tcgcggccgg gcaggaggct gcacgagctg gcgaggacgg
      481 cggcgaccag ggcgagcgcc gcggctcggt tccgggttcg agaggtcggt ggcatggagt
      541 ggctcctcgc gggcctggag ggagcaggcc cgatcacggc tccctgtcgg cctcggcgcg
      601 gcgaagttga gtcgctggcg agcccgacgt cgccgcgctc gcgccgggct cctcacgacc
      661 cgaaaaacgg tggtggcccc gagtaaccga cggggtttcc ctcgccagct cctcggggcc
      721 gaccgttgcg agctgcccgg ggtcacgccc gggggcggtt cctcgcgcac ctcgctcctc
      781 cggccttcgg ccgccgtgac ccagaggcct cggcgccggt cgaccggcgg gtgccggatc
      841 cctaccaccg cggcctgaag gccccggtgc ctggtaccgg cgatgctggt cctccggcct
      901 cgtgacccga aggccccggt gccgagcaac cagcgaggtc tcgtctccgg tgcttcggcc
      961 cgaaggcctc ggcgctggat cggcgacccg gacccggact ccggcctcgc cacccggagg
     1021 cggcggtgtc gggttccgaa cccaccaggt gccggttgcg accctcccac cgaacccacc
     1081 cccggtggtc cttcccagca agctggtggg gacctccgtg ggcttcctgc ccggtgctcg
     1141 agccgctccg tgcggcctgg cgagggacac catgcgcccc gccggcagac gggtcaagca
     1201 ctttttcgcc gtccccaggg ttgtccccgg accttttccg ttccccccag gttcacccgt
     1261 caggtgcaca gggtcgtgca caatccatcc acagggaacc ggcggtcctg gaacggtccc
     1321 gacccacgag ccgacaccgc gtcgtccggt ctcgtcgggg tagacgggat cagtaccagg
     1381 cgccgcggct ccgcagcacc tcgcgcagca ccgcagggcg gtcggtcatg atcccgtcga
     1441 cgccgaggtc gagcagcagg tgcatctcct cggcgtcgtc gatggtccag acgtggacct
     1501 gcagcccggc ggcgtgggcc cgctcgacga agcgccggtc ggtgacggcg acgcccttgt
     1561 acttgtgcgg cacctgggcg cagccgccgg tgatccgccc cgccggaagc ccgaacgagc
     1621 cgccgcgcag gcgggcgatg gccttgggcc cgagcgacgt gcagaggcgg gggccgaggg
     1681 ccgaacgaag gcggttgatg cgtgcatcgg agaacgagcc gacgcacaca cgatcgaccg
     1741 actccgtccg ctcgatcacc gaggcgagcg gctcgacggc ggcgtcgtgc ttcggatcga
     1801 tgttgacgcg gaggtcgggg aacgcggcga tgaggtcctc gagcagcggg atcggctcgc
     1861 gtccgtcgac gcgcgcccgg cggacctcgt cgtaggtcag ctcggcgatg acaccggtcc
     1921 ggtccgtgac gcggtcgagc cggtcgtcgt ggaacgcgag gcagatcccg tcggcggtca
     1981 cgtgggcgtc ggtctccacg tagcggtagc cgagctcgac cgcgccctcg aacgccggca
     2041 tcgtgttctc gggccagtca ccggctccgc cgcggtgggc gaacgcgagg gggccggggt
     2101 gatcgaggaa gggccaggga ctcgacggca cgtggggagg gtagcggtgc cgttcggcgg
     2161 gaacggtgat aagaccgtcg gtgcacaccg tcgtccccca ac
//
LOCUS       DATMFS010000573         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_345931, whole genome shotgun sequence.
ACCESSION   DATMFS010000573 DATMFS010000000
VERSION     DATMFS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345931"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VNQ33_10885"
     CDS             <1..1426
                     /locus_tag="VNQ33_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98656.1"
                     /translation="AGQLSDLEIQPMRYEAPSAPSGLSTGAAGAATAQPEILPRSSYT
                     SKGWAYGNAGCSGGPVTASGGVKYAVVHHTTNSNSYAASDVPAMLASIYTYHTGTNGW
                     CDTGYNFIVDRFGRIWEGRSGGIAKAIVGGHAQGFNTGSVGVSFLGQYEPGASPTAAL
                     PSDAALAAAAKVIGWKLSLNGIDPTGTIAVTSGGSNKYPAGTAVTLNRVIGHRDVGQT
                     ACPGASLYTKLPSIRTAAKAYQGGGTATTIPTEPPPAAYAPFYSADQLVAQQYRDVLL
                     REPAASDLSYWSARVGNSWTPGQFIAQLETSTEADNRVHAVTRLYRAYFLRNPDHSGQ
                     TFWLARRGEGRTLVKISQSFATSSEFIRNYGSLSNAGFVDRVYRNVLGRGPDAGGAAY
                     WKGKLDTGTPRGQVMANFSQSSEYLRKTNNGVYVVGIHEALLQRAAATDVYTLYEAAL
                     TNGSTSLTALSNTLFSSTEYQNRF"
     gene            complement(1439..1690)
                     /locus_tag="VNQ33_10890"
     CDS             complement(1439..1690)
                     /locus_tag="VNQ33_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98657.1"
                     /translation="MTRADQDPTSAPSLKARAKRLAAPVVAKAREEVVKATADDQLAL
                     RAEVAELRAELVRQRAEHEADLAAVNEELHALAAAQRKR"
     gene            complement(1687..2598)
                     /locus_tag="VNQ33_10895"
     CDS             complement(1687..2598)
                     /locus_tag="VNQ33_10895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ98658.1"
                     /translation="MTVLDDPAAAGSAGDAERPVLALLAAPGTSGRAIPLLGLLGIHA
                     RVVSWHRRGALVPDGVLVTSVDSLRSLSTAPPAPVAVWVKHHDQIDRATEDGAALLLT
                     ANPDLVARGAVLVPPVAIEVDRWPAVAPLVRRRRREAAGLPAVHVLRVEGGVEPLGGE
                     RELAMASAAVVSGPATLLALALGTPVVTSPETVRRLGLRPGRDAEVAGGPDEAMERAE
                     AIAHDDAWAAALSRHARRRAEDHLDLGRPAREVARRLGLAPSPAGAVARVDDRLAELA
                     TPAGSPTRARVADALAVLTADGGGSSA"
     gene            complement(2595..>3087)
                     /locus_tag="VNQ33_10900"
     CDS             complement(2595..>3087)
                     /locus_tag="VNQ33_10900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98659.1"
                     /translation="EANKRPGLFLDAMASMGEAVDGVLAGKGSLADELAASAERLGLA
                     DRVSLPGFVPDDDLVELFRQATAVVYAPYDEDYGFVTLQAFLAGVPVVTTDDSGGVLE
                     WVEHEVTGLVTDGTPGAMGAALDRLVRDPDLARRLGAAGRDRVKDLRWEHVVATLLGG
                     APR"
BASE COUNT          435 a         1201 c         1076 g          375 t
ORIGIN      
        1 cgccggccag ctctccgatc tcgagatcca gccgatgcgc tacgaagcgc cgtccgcccc
       61 gtccggcctg tccaccggcg cggccggcgc cgccaccgcc caacccgaga tcctgccccg
      121 cagcagctac acctcgaagg gctgggcgta cggcaacgcc ggctgctcgg gcggtccggt
      181 caccgcatcg ggcggcgtga agtacgccgt ggtgcaccac accacgaact cgaacagcta
      241 cgccgcctcg gacgtgcccg ccatgctcgc gtcgatctac acgtaccaca ccggtacgaa
      301 cggctggtgc gacaccgggt acaacttcat cgtcgaccgg ttcggccgga tctgggaggg
      361 ccgctccggc ggcatcgcca aggcgatcgt cggcggccac gcccagggct tcaacaccgg
      421 cagcgtcggt gtctcgttcc tcgggcagta cgagcccggc gcctccccca ccgccgccct
      481 gccgagcgac gccgcgctgg cggccgccgc caaggtgatc ggctggaagc tgagcctcaa
      541 cggcatcgac cccacgggca cgatcgcggt cacgagcggc ggctccaaca agtaccccgc
      601 cggaacggcc gtcacgttga accgggtgat cgggcaccgc gacgtcggcc agaccgcctg
      661 ccccggcgcc agcctctaca ccaagctgcc gagcatccgc accgccgcca aggcgtacca
      721 gggcggcggc accgcgacca ccatcccgac cgaaccgccg ccggccgcct acgcaccctt
      781 ctactcagcc gaccagctcg tcgcccagca gtaccgcgac gtgctgctcc gcgagcctgc
      841 cgcttcggac ctcagctact ggtcggcccg cgtcggcaac tcctggaccc ccggccagtt
      901 catcgcccag ttggagacat ccaccgaggc cgacaaccgg gtccacgccg tcacccggct
      961 ctaccgggcc tacttcctgc gcaaccccga tcactccggg cagacgttct ggctcgcccg
     1021 gcgcggcgag ggccggaccc tcgtcaagat ctcgcagtcg ttcgccacgt cgtcggagtt
     1081 catccggaac tacgggtcgc tctccaacgc ggggttcgtc gaccgggtgt accggaacgt
     1141 cctcggtcgc ggccccgatg ccggcggcgc cgcctactgg aagggcaagc tcgacacggg
     1201 aacccctcgc ggccaggtca tggccaactt ctcgcagtcg agcgagtacc tccgcaagac
     1261 gaacaacggc gtctacgtgg tcggcatcca cgaggccctg ctccagcggg cggctgccac
     1321 cgacgtgtac acgctctacg aagccgccct gaccaacggc tccacgtcgc tcacggcgct
     1381 gtccaacacc ctcttcagca gcaccgagta ccagaaccgg ttctgaccgc tcgagggttc
     1441 agcgcttgcg ctgagcagcg gcgagggcgt gcagctcttc gttgacggcg gcgaggtcgg
     1501 cttcgtgctc ggcgcgctgg cgcaccagtt cggcgcgcag ctcggccacc tcggcgcgca
     1561 gcgccaactg gtcgtcggcg gtggccttga ccacctcctc gcgagccttc gccaccaccg
     1621 gcgcggccag gcgcttggcg cgcgccttga ggctgggggc gctggtgggg tcctgatccg
     1681 ctcgggtcac gccgacgatc ctccaccatc tgcggtgagc accgccagcg catcggccac
     1741 ccgggcgcgc gtcggcgatc ccgccggggt ggcgagctcg gccaggcggt cgtcgacgcg
     1801 ggcgacggcg ccggcgggcg acggcgccag gcccagccgc cgagcgacct cacgggcggg
     1861 gcggccgaga tcgaggtggt cctcggcgcg ccggcgcgcg tggcgggaca gcgccgcggc
     1921 ccacgcatca tcgtgggcga tcgcctcggc ccgctccatc gcctcgtccg gaccgccggc
     1981 cacctcggcg tcgcgccccg ggcgcaggcc gaggcgccgg acggtctcgg gactcgtgac
     2041 caccggcgtg cccagtgcca aggcgagcag ggtggccggg ccgctgacga ccgcggccga
     2101 cgccatcgcc agctcccgct cgccgccgag cggctcgacg ccgccttcga cgcgcagcac
     2161 gtggaccgcc gggagcccgg ccgcctcacg ccgtcggcga cggacgagcg gcgccacggc
     2221 cggccagcga tccacctcga tggcgaccgg tgggaccagg accgcccccc gggcgacgag
     2281 gtcggggttc gcggtcaaga ggagcgcggc cccgtcctcg gtggcccggt cgatctggtc
     2341 gtggtgcttc acccagacgg cgacgggcgc gggcggtgcg gtggacaggg agcgcagcga
     2401 gtcgaccgag gtgacgagca cgccgtcggg gacgagggcg ccgcgccggt gccacgacac
     2461 cacccgggcg tggatgccga gcaggccgag cagcgggatc gcccgtcccg aggtccccgg
     2521 cgcggcgagc agcgccagga ccggtcgctc ggcgtcgcct gcggacccgg cggcggccgg
     2581 gtcgtcgagc accgtcaccg cggcgcaccg ccgagcaggg tggcgaccac gtgctcccag
     2641 cgcaggtcct tcacccggtc gcgcccggcg gcgccgagtc gccgggcgag gtcgggatcg
     2701 cgcacgagcc ggtcgagcgc cgcgcccatc gcgcccggcg tgccatcggt gacgagcccg
     2761 gtcacctcgt gctcgaccca ctcgaggacg ccgccgctgt cgtcggtggt cacgaccggg
     2821 accccggcca agaaggcctg gagggtgacg aagccgtagt cctcgtcgta gggcgcgtag
     2881 accacggccg tggcctggcg gaagagctcg acgaggtcgt cgtcggggac gaaacccggg
     2941 agcgacaccc gatcggcgag gccgagccgt tcggcgctgg cggccagctc gtcggccagc
     3001 gagcccttgc cggcgagcac cccgtcgacc gcctcgccca tcgacgccat ggcgtcgagg
     3061 aacaggccgg gccgcttgtt cgcctcc
//
LOCUS       DATMFS010000574         5485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_136075, whole genome shotgun sequence.
ACCESSION   DATMFS010000574 DATMFS010000000
VERSION     DATMFS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136075"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VNQ33_10905"
     CDS             <1..606
                     /locus_tag="VNQ33_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98660.1"
                     /translation="LDHLTGCDDCRQALARLTETVDAVIQTAPEAEPPSGFEVRALAS
                     FDGAVPASAASGSRSAWRRVALAAAVLLAIAASAAAIGAVASRDGGGDQTAADRSSAA
                     LTTPGGAEVGTVRVERRTVTGGGWSSDLVVSVDAGARQGTYRVECDYESGGPYRAGEL
                     EVGPDGVTGWRTTVSVPTYDLSRVRLVSTGNADNLEAEFGA"
     gene            670..2283
                     /locus_tag="VNQ33_10910"
     CDS             670..2283
                     /locus_tag="VNQ33_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98661.1"
                     /translation="MAEHTTDGGSAATDGTETADPDVIAQLRAVTSLLESLVQDRTPL
                     DALDPAERARLLNAAGDLFHPDVEARRQLTKARRRQARQARLAREEEVLAGTGIRSLR
                     AKPVFTTPNVFAPEGMVATGPGDAGAGAAQETARATTGEADRHCYVCKRDYREVHRFY
                     DQLCPSCAAFNEHKRTESADLSGRVALLTGGRVKIGYQAGIKLLRAGAELIVTTRFPR
                     DSAMRYAQEPDFGQWGHRLEIYGLDLRHTPSVEALCHHLDTTRGRLDFIVNNACQTVR
                     RPPGFYRHMMELETGSLTTMPAEAQALLGGVDGLRSPELLEGPADLLESASLAVESAS
                     TSSTSSAEMSQAALLPEEHEPQAHLFPAGRLDQDLQQVDLRGRNSWRLTLAEVSSVEL
                     LETQLVNAVAPFVLNARLKPLMLRTSERDKHIVNVSAVEGQFYRRMKTTRHPHTNMAK
                     AALNMMTRTSAADYHADGIHMNSVDTGWVSDEDPIEITERKRAEHRFHPPLDIVDGAA
                     RIIDPIIDGFNTGTHVWGQFLKDYRPTDW"
     gene            complement(2354..4021)
                     /locus_tag="VNQ33_10915"
     CDS             complement(2354..4021)
                     /locus_tag="VNQ33_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWJ98662.1"
                     /translation="MQGLMQDYPLTLPHIFHRVERLFPDKEIVTALPTGRERTTYGDW
                     AVRTRKLGGVLNDLGISPDGRVATFSWNTSRHLELYFAAPCTGRVLHTLNLRLFPEQI
                     TYIVNHAEDEVIFVDKTVAGLIWPLLPTFTTVRHVVVIDDGGPFDTSEVPEGIELHDY
                     EALLDAASPVEFHVEDENLAASMCYTSGTTGNPKGVVYSHRSTMLHTMAAMFADGFGA
                     RESDRILPVVPMFHANAWGLAHAAVACGAELIMPGPNLAPEPLAELIESEKVTVAAGV
                     PTIWMGVLPALKGRDTSALRVIPCGGSAVPKALSEAFREQLGLPIYQAWGMTETSPVA
                     TVGEIKSKLRDLPEEELADIRAMQGLPLFGVELRVLSQEDGSPVPWDGEARGELQAAG
                     PWIAREYYDDDRSPESFTEDGWLRTGDVATINPDGYIRLVDRTKDVVKSGGEWISSVE
                     LENEIMAHPAVAEAAVIGVSHPKWQERPLACVVLKEGESATKEDILEFLDGRVAKWWL
                     PDDVVFIDEVPKTSVGKFSKKDLRTRFEGYELPTVEASRHDGDPSSR"
     gene            4160..4921
                     /locus_tag="VNQ33_10920"
     CDS             4160..4921
                     /locus_tag="VNQ33_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWJ98663.1"
                     /translation="MDDTSPAVDAADARPAATGPPSKAARNATIGFLVFLVVMSNLGN
                     ILFASFVNEHPGWLIAMNASNRNLALASNNLDPLAFYGIGFLRLFAPDLFFFWIGRWY
                     GDAAIRWMERKAPTYGELLRQLETWFDKARFAVVAIAPNNPVCLFAGAAGMTYAAFLV
                     ADVVGTIVRLVLIRAFSSVFEDLLGSIKDFIGDYRWPITVLSIVLVGLTIWSDRRGGR
                     DGIGDLVNLEEGMAEAEAELEGEATMDASDARQAE"
     gene            4932..>5485
                     /locus_tag="VNQ33_10925"
     CDS             4932..>5485
                     /locus_tag="VNQ33_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98664.1"
                     /translation="MTAPTATAPLPPPTKRQRNLVFGAIGAAIVIGWIGDALWASLVD
                     RSPITLLLLNAKPRYQILVVNQLAVWVFFPVALARLLCTKPLLWLAGGWYGPRMADWV
                     QGRTERGGRLVRWAQRHFPKYGWMVMIITTSNPVCLLAGSVGYPLGMLMLWAVIGTSI
                     RLAAIIVVGDIFSAAISAFIDWVV"
BASE COUNT          795 a         1991 c         1886 g          813 t
ORIGIN      
        1 ctcgaccacc tgaccggctg cgacgactgc cgccaggccc tggcccgcct caccgagacg
       61 gtcgatgccg tcatccagac cgcccccgaa gccgagccgc cgagcgggtt cgaggtgcgc
      121 gccctcgcct cgttcgacgg cgcggtcccc gcgtccgctg cctccggcag ccgttcagcc
      181 tggcgccggg tcgccctcgc cgccgcggtg ctcctcgcca tcgctgcttc tgccgcggcc
      241 atcggggccg tggccagccg cgacggcggc ggcgaccaga cggccgcgga tcgctcgtcg
      301 gccgcgctca ccacgccggg cggcgccgag gtcgggacgg tccgcgtcga gcgccggacc
      361 gtcaccggcg gcggctggag ctcggacctg gtggtgtcgg tcgacgccgg cgcccgccag
      421 ggcacgtacc gggtggagtg cgactacgag tccggcgggc cctaccgagc cggcgagctg
      481 gaggtcggcc cggacggcgt gaccgggtgg cggacgaccg tgtccgtccc gacctacgac
      541 ctgagccggg tccggctcgt gagcaccggc aacgccgaca acctcgaagc cgagttcggc
      601 gcctgatcgc cggcgcggcg aagcagccgg ggtgggactg gggcacggca ggccccgtag
      661 ggtcggtcga tggccgagca cacgacagac ggtggatctg cggccaccga cggcaccgag
      721 acggccgatc cggacgtgat cgcacagctg cgtgcggtca cgtccctgct cgagtcgctg
      781 gtgcaggacc ggacgccgct cgatgccctc gaccccgccg agcgggcccg actgctcaac
      841 gcagccggcg acctgttcca ccccgacgtg gaggctcgcc gccagctcac caaggcccgc
      901 cgccgccagg cccgccaggc ccgcctcgcc cgcgaggagg aggtgctcgc cgggaccggg
      961 atccggtcgc tgcgcgccaa gccggtcttc accaccccga acgtgttcgc ccccgagggc
     1021 atggtcgcca ccggtccggg cgatgccggt gccggtgccg cccaggagac ggcgcgcgcc
     1081 acgaccggcg aggccgaccg ccactgctac gtctgcaagc gcgactaccg cgaggtgcac
     1141 cgcttctacg accagctctg cccgtcgtgc gccgcgttca acgagcacaa gcggaccgag
     1201 tcggccgacc tgtcgggccg ggtcgcgctg ctcaccggcg gccgggtgaa gatcgggtac
     1261 caggccggga tcaagctgct gcgcgccggc gccgagctca tcgtgacgac ccggttcccc
     1321 cgggactcgg ccatgcgcta cgcccaggag cccgacttcg gccagtgggg ccaccgcctc
     1381 gagatctacg gcctggacct gcgccacaca cccagcgtcg aagccctctg ccaccacttg
     1441 gacacgaccc gcggccggct cgacttcatc gtcaacaacg cctgccagac ggtccgccga
     1501 ccgcccgggt tctaccgcca catgatggag ctggagaccg gctcgctcac cacgatgccc
     1561 gccgaggccc aggccctgct cggcggcgtc gacggcctcc gcagccccga gctgctcgag
     1621 ggaccggccg acctgctcga gtcggcatcg ctcgcggtgg agtcggcgtc gacgtcatcg
     1681 acctcatcgg cggagatgtc gcaggccgcc ctgctgcccg aggagcacga gccccaggcc
     1741 cacctcttcc ccgccggccg gctcgaccag gacctccagc aggtcgacct ccggggccgg
     1801 aactcgtggc gcctcaccct ggccgaggtg tcgtcggtgg agctgctcga gacccagctc
     1861 gtgaacgcag tggcgccgtt cgtgctgaac gcgaggctca agccgctgat gctccgcacg
     1921 tccgagcgcg acaagcacat cgtcaacgtg tccgccgtgg agggccagtt ctaccggcgc
     1981 atgaagacca cccggcaccc gcacacgaac atggccaagg ccgcgctcaa catgatgacc
     2041 aggacctcgg cggccgacta ccacgccgac gggatccaca tgaacagcgt cgacaccggc
     2101 tgggtgagcg acgaggaccc gatcgagatc accgagcgca agcgggccga gcaccgcttc
     2161 cacccgccgc tcgacatcgt ggacggcgcc gcccgcatca tcgacccgat catcgacggc
     2221 ttcaacaccg gcacccacgt ctggggccag ttcctcaagg actaccgccc caccgactgg
     2281 tgaacgggag gggcggccca agcccgaact tcgtaggtga gcgcatccaa gacgtcgatc
     2341 tgacctacga agttcaccgc gaggatggat cgccgtcgtg acgtgacgcc tccaccgtgg
     2401 gcagttcgta cccctcgaac ctcgtgcgga gatccttctt ggagaacttg ccgacgctgg
     2461 tcttggggac ctcgtcgatg aagacgacgt cgtcggggag ccaccacttg gcgacgcggc
     2521 cgtcgaggaa ctcgaggatg tcctccttgg tggcggactc cccctccttc agcaccacgc
     2581 aggcgagcgg gcgctcctgc cacttggggt ggctgacgcc gatgacggcg gcttcggcga
     2641 cggccgggtg ggccatgatc tcgttctcga gctccaccga gctgatccac tcgccgccgc
     2701 tcttgaccac gtccttggtg cggtcgacga ggcggatgta gccatcgggg ttgatggtgg
     2761 cgacatcgcc ggtgcgcagc cagccgtcct cggtgaagga ctcgggcgag cggtcgtcgt
     2821 cgtagtactc ccgggcgatc cacgggccgg ccgcctggag ctcgccccgg gcctcgccgt
     2881 cccacggcac cggcgagccg tcctcctggg agagcacccg cagctcgacg ccgaacaggg
     2941 gcagtccctg catggcgcgg atgtcggcca gctcctcctc gggcaggtcg cggagcttgg
     3001 acttgatctc gccgacggtg gccaccggcg aggtctcggt catgccccag gcctggtaga
     3061 tcggcaggcc gagctgctcg cggaacgcct cggacagcgc cttgggcacg gccgagccgc
     3121 cgcacgggat gacccgcagc gccgaggtgt cacggccctt cagcgccggc aggacgccca
     3181 tccagatggt ggggacaccg gcggcgacgg tgaccttctc ggactcgatc agctcggcca
     3241 ggggctcggg cgccaggttc gggccgggca tgatcagctc ggccccgcag gccacggcgg
     3301 catgggcgag tccccaggcg ttggcgtgga acatggggac caccggcaag atgcggtcgg
     3361 actcccgggc gccgaagccg tcggcgaaca tcgccgccat ggtgtggagc atcgtcgagc
     3421 ggtggctgta gacgacaccc ttcgggttgc ccgtggtgcc ggacgtgtag cacatcgacg
     3481 cggcgaggtt ctcgtcctcg acgtggaact cgacgggtga cgcggcgtcc agcagcgcct
     3541 cgtagtcgtg gagctcgatc ccctcgggga cctcggaggt gtcgaacggg ccgccgtcgt
     3601 cgatgaccac gacgtggcgg acggtggtga acgtcggcag cagcggccag atcaggccgg
     3661 ccaccgtctt gtcgacgaag atcacctcgt cctcggcgtg gttcacgatg taggtgatct
     3721 gctcggggaa caggcggagg ttgagcgtgt ggagcacgcg gccggtgcac ggagcggcga
     3781 agtagagctc caggtggcgt gacgtgttcc acgagaacgt ggcgacgcgg ccgtcgggcg
     3841 agatcccgag gtcgttcaag acgccgccga gcttgcgcgt gcggacggcc cagtcgccgt
     3901 aggtggtgcg ctcgcggccg gtggggagcg cggtgacgat ctccttgtcg gggaacaggc
     3961 gctccacccg gtggaagatg tgcgggaggg tgagcgggta gtcctgcatc aggccctgca
     4021 tgtgtctgtc cccttgcgtt cggtccgacg gcttgtctcg gaacctagcc aagccggtcg
     4081 gcgcctgtcc tccggcgctc ccaggcagtc cgcggtgggt cgaccggacc gcggcgaccc
     4141 gcctgccaca cttgtcccct tggacgacac ctccccagcg gtcgacgccg ccgacgcgcg
     4201 gccggccgcg accggtcctc cgtcgaaggc ggcgcgcaac gccaccatcg ggttcctcgt
     4261 gttcctggtg gtgatgtcga acctgggcaa catcttgttc gcgtcgttcg tgaacgagca
     4321 ccccggctgg ctcatcgcca tgaacgcgtc gaaccgcaac ctggcactgg cgtcgaacaa
     4381 cctggatccc ctggcgttct acggcatcgg gttcctgcgc ctgttcgcgc cggacctgtt
     4441 cttcttctgg atcggccgct ggtacggcga cgcggccatc cgctggatgg agcgcaaggc
     4501 gcccacctac ggcgaactgc tgcgccagct cgagacttgg ttcgacaagg cccggttcgc
     4561 ggtagtggcc atcgccccca acaacccggt gtgcctcttc gccggagcgg ccggcatgac
     4621 ctacgcggcg ttcctcgtgg ccgacgtcgt cggcaccatc gtccggctcg tcctcatccg
     4681 ggccttcagc agcgtgttcg aagacctgct gggctccatc aaggacttca tcggcgacta
     4741 ccgctggccc atcaccgtcc tgtcgatcgt gctcgtcggc ctcaccatct ggtccgaccg
     4801 ccgcggcggg cgcgacggca tcggcgacct ggtgaacctc gaggagggca tggccgaggc
     4861 cgaagccgag ctggagggcg aggccaccat ggacgccagc gacgcccgac aggcggagtg
     4921 acgacgcgac gatgacggcc cccaccgcca ccgctcccct gccaccgccc accaagcgcc
     4981 agcgcaacct ggtgttcggc gccatcggcg cggccatcgt catcggctgg atcggcgacg
     5041 cgctctgggc gtcgctcgtg gatcgcagcc cgatcacgct gctgctcctc aacgccaagc
     5101 cccgctacca gatcctggtg gtcaaccagc tggccgtctg ggtcttcttc ccggtggccc
     5161 tggcccgcct cttgtgcacc aagcctctgc tgtggctcgc cggcgggtgg tacggccctc
     5221 ggatggccga ctgggtccag gggcgcaccg agcggggcgg ccggctcgtg cgctgggcgc
     5281 agcggcactt cccgaagtac gggtggatgg tcatgatcat caccacgtcg aacccggtgt
     5341 gcctcctcgc cgggtccgtc ggctacccgc tcggcatgct gatgctctgg gcggtgatcg
     5401 gcacctcgat ccgcctcgcg gcgatcatcg tggtcggtga catcttctcg gccgccatct
     5461 ccgccttcat cgactgggtc gtcga
//
LOCUS       DATMFS010000575         3713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_65566, whole genome shotgun sequence.
ACCESSION   DATMFS010000575 DATMFS010000000
VERSION     DATMFS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65566"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VNQ33_10930"
     CDS             complement(<1..1568)
                     /locus_tag="VNQ33_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ98665.1"
                     /translation="MAALDLFSPPVRDWFATSFPAPTQAQEEGWPAIASGGHTLILAP
                     TGSGKTLTAFLWGIDRLLTTPPPGAEARTRLLYISPLRALAVDVEKNLRAPLVGIAHA
                     AERAGHPVNVPTVGTRTGDTPADERRRLARNPPDLLITTPESLYLMLTSAARESLVNV
                     EAVIIDEIHALAATKRGSHLTLTLERLEARCAKPPQRIGLSATQRPLEEIATFLGGFE
                     SPGKRRPVTVIDAGSRKPLEIEVVVPVEDMGSLGTVVEGPVGGGPAAAGPVRRSIWPA
                     MHPRLLELVQEHRSTLIFVNARRLAERLATRLNELAAEGADREAGTEPADSTASRFVA
                     AEAVGVPPPEVELVKAHHGSLSRERRLIIEDELKSGRLRGLVATSSLELGIDMGAVDL
                     VIQVESPGAVSRGLQRIGRAGHQVGEPSKGKVFPKHRADLVEAAVVTQRMHEGLIEHT
                     HYPRNPLDVLAQQVVASVALDELKVSELAALVRRAAPFAELSDEVWEAVLDLLAGRYP
                     SEEFAELRPRVVWDR"
     gene            complement(1622..2173)
                     /locus_tag="VNQ33_10935"
     CDS             complement(1622..2173)
                     /locus_tag="VNQ33_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98666.1"
                     /translation="MSRSDRTEATTTTTEDLFATVGELDSPPSPSTSTKADYVAAFET
                     YLTQGDVTDGDLVFDDDQAACLAPKWVRAITVDRLREAGVDADDVVDMDFVELELGEP
                     VARTMVDGFAACDIDVASVAAGALSASESSDEQVCARKEVDPTLVDDLFIAGFSGSSQ
                     RVIDAAYDALVAQLVEACDDGSD"
     gene            complement(2287..2922)
                     /locus_tag="VNQ33_10940"
     CDS             complement(2287..2922)
                     /locus_tag="VNQ33_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98667.1"
                     /translation="MRKLLVLTCAITLALVVAGCGSSGGSDGAEGTTTTKGDATTTTA
                     ADDTSTTAGDGDVAPTDVTAEQYAAAFAAGLTSGDADGTDLVLPKDAAECVAPKFVDI
                     VTVETLNQAGITAEDASESGFEPGGLGLDASQGAAIVAAFGECHFDIYAELAKGLTAG
                     LGEDVQQCTAANIDHDLADAMLAKTFSTGENDAEFEALLADLQKTCDLPAN"
     gene            2984..3652
                     /locus_tag="VNQ33_10945"
     CDS             2984..3652
                     /locus_tag="VNQ33_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWJ98668.1"
                     /translation="MAEFQSREYHPAFEEYCEAIFELHEDDVEVIQARIAERLGVSRP
                     AVSEMTRRMESEGLVEIDRTIRLTGSGSVLAERVVRRHRLAERLLTDILGLSWADAHT
                     EAGRWEHVISDAVEAAIIRLLDDPTTCPHGNPIPGTDYQAPVTVPLSDLGVGQGFTVM
                     RIPEELEFTPGLLEFLEDASIQPGHTGTVTASSPDGTTTVEIDGRHVGVGSFASARIL
                     VANA"
BASE COUNT          519 a         1350 c         1308 g          536 t
ORIGIN      
        1 cggtcccaca cgacgcgggg ccgcagctcg gcgaactcct ccgacgggta gcggccggcg
       61 agcaggtcca gcaccgcctc ccacacctcg tcgctcagct cggcgaacgg ggccgcccgg
      121 cggacgaggg cggccagctc cgagaccttg agctcgtcga gcgccaccga agccaccacc
      181 tgctgcgcca agacgtcgag cgggttgcgg gggtagtgcg tgtgctcgat gagcccctcg
      241 tgcatgcgct gggtcacgac ggccgcctcg acgaggtcgg cgcggtgctt cgggaacacc
      301 ttccccttgc tgggctcgcc gacctggtgg ccggcccggc cgatgcgctg caagccgcgc
      361 gacaccgcgc ccggcgactc cacctggatg acgaggtcga ccgcccccat gtcgatcccg
      421 agctccagcg acgaggtggc gacgaggccg cgcaggcgcc cggacttgag ctcgtcttcg
      481 atgatcagcc gccgctcccg tgacagcgaa ccatggtgcg ccttcaccag ctccacctcc
      541 ggcggcggga cgccgaccgc ctcggcggcc acgaaccgag acgccgtcga gtcggccggt
      601 tcggtgccgg cctcgcggtc tgctccctca gccgccagct cgttgagccg tgtggccaac
      661 cgctcggcca ggcggcgggc gttgacgaag atcagggtgg accggtgctc ttgcaccagc
      721 tccagcaggc gggggtgcat cgccggccag atcgacctgc ggacgggccc ggctgcggcc
      781 gggccgccgc ccaccgggcc ctcgaccacc gtccccagcg accccatgtc ctccaccggg
      841 accacgacct cgatctcgag cggcttgcgg ctgccggcat cgatgaccgt gaccggtcgc
      901 cgcttgccgg gtgactcgaa gccgccgagg aacgtggcga tctcctccag gggccgctgg
      961 gtggccgaca ggccgatgcg ctgcggcggc ttggcgcacc gggcctccag ccgctccagc
     1021 gtgagggtca ggtgcgaccc ccgcttggtg gcggcgaggg cgtggatctc gtcgatgatc
     1081 accgcctcga cgttgaccag cgactcccgc gccgccgacg tgagcatcag gtagagcgac
     1141 tcgggcgtgg tgatcagcag atccggaggg ttgcgggcga ggcggcgccg ctcgtcggcc
     1201 ggcgtgtcac cggtgcgggt ccccaccgtg ggcacgttga cggggtgccc ggcgcgctcg
     1261 gcggcgtggg cgatgccgac cagcggggcc cgcaggttct tctcgacgtc gacggccagg
     1321 gcccgcagcg gcgagatgta cagcaggcgg gtccgcgcct cggcgccggg cggcggggtc
     1381 gtgagcaacc ggtcgatgcc ccacaggaac gccgtcaggg tcttgcccga cccggtgggc
     1441 gccaggatca gcgtgtgccc gccggacgcg atggccggcc acccctcctc ctgtgcctgg
     1501 gtcggcgcag ggaacgaggt ggcgaaccag tcccggacgg gaggcgagaa gaggtcgagc
     1561 gcagcgatcc ccgaagccta cggagcacgt ctgacagcaa gaccgggccc gtctgccacg
     1621 gtcagtccga cccgtcgtcg caggcctcga cgagctgcgc gaccaaggca tcgtacgccg
     1681 cgtcgatcac ccgctgactg ctaccggaga atccggcgat gaaaaggtcg tccacgagcg
     1741 tcggatcgac ctccttgcgg gcgcagacct gttcgtcgga ggactcggac gcggacaggg
     1801 ccccggcggc gaccgacgcg acgtcgatgt cgcacgcggc gaagccgtcg accatcgtgc
     1861 gagcgaccgg ttcaccgagc tcgagttcca cgaagtccat gtcgaccacg tcgtcggcgt
     1921 cgacaccggc ctcccgcaac cggtcgacgg tgatcgcccg tacccacttc ggggcaaggc
     1981 acgctgcctg gtcgtcgtcg aagacgaggt cgccgtcggt gacgtcgccc tgcgtcaggt
     2041 acgtctcgaa cgccgccacg tagtcggcct tcgtcgaggt ggatggcgac ggcgggctgt
     2101 ccagctcgcc gaccgttgcg aagaggtctt ccgtggtcgt cgtggtggcc tcggtccggt
     2161 ccgagcggct catcgtggta gctgcctcac cgtcgcttcc tgccgaggtg ccgcagcccg
     2221 tggccgcagc gacgaggccg agggccacga gcccgaggag caaccggttc cgccgtggcg
     2281 tgcgcatcag ttcgccggga ggtcgcaggt cttctggagg tcggccagca gggcttcgaa
     2341 ctcagcgtcg ttctccccgg tgctgaacgt cttggccaac atggcgtcgg ccaggtcgtg
     2401 gtcgatgttc gcggccgtgc actgctgcac gtcctcgccc agcccggcgg tcaggccctt
     2461 cgccagttcg gcgtagatgt cgaagtggca ctcgccgaag gccgcgacga tcgcggcgcc
     2521 ctgcgacgca tcgagcccca ggccaccggg ctcgaagccg gactccgacg catcctccgc
     2581 ggtgatccca gcctggttga gcgtctcgac ggtgacgatg tcgacgaact tcggcgccac
     2641 gcactcggcg gcgtccttcg gcagcaccag gtcggtgccg tccgcgtcac ccgacgtcag
     2701 gcccgccgcg aaggcggcgg cgtactgctc ggccgtcacg tcggtgggcg ccacgtcgcc
     2761 gtcccccgcg gtggtggagg tgtcgtcggc cgccgtggtg gtggtcgcat cgcccttggt
     2821 cgtggtggtg ccctccgccc cgtcggatcc acccgaggac ccgcacccgg cgacgaccag
     2881 ggcgagggtg atggcacacg tgaggaccaa gagcttccgc atccccggac cctagatgac
     2941 cggtgggagc ccttctcgtc gatcgaggcg gtaccctcgc tgggtggccg agttccaatc
     3001 acgtgagtac caccccgcgt tcgaggagta ctgcgaagcg atcttcgagc tccacgagga
     3061 cgacgtggag gtgatccagg cgcgcatcgc cgagcgcctc ggcgtgtcgc ggcccgccgt
     3121 gtcggagatg acccggcgca tggagtccga gggcctcgtc gagatcgacc gcaccatccg
     3181 gctcaccggt tccggctcgg tgctggccga gcgggtcgtc cgccgccacc gcctggccga
     3241 gcgcctgctc accgacatcc tcgggctgtc gtgggccgat gcccacaccg aggccgggcg
     3301 ctgggagcac gtgatctccg acgccgtcga ggccgcgatc atccgcctcc tcgacgaccc
     3361 caccacctgc ccgcacggca accccatccc cggcacggac taccaggcgc ctgtgacggt
     3421 cccgctgtcg gacctcggcg tcggccaggg cttcaccgtg atgcgcatcc ccgaggagct
     3481 ggagttcacg cccggcctcc tcgagttcct ggaggacgca tcgatccaac cgggccacac
     3541 cggcacggtc acggccagct cgcccgacgg caccaccacc gtggagatcg acgggcgcca
     3601 cgtgggcgtc ggctccttcg ccagcgcccg catcctcgtg gccaacgcct gactgtgaag
     3661 atcagcgagg gcctggcgtt cgtgctgggc tgtcgccgac agcagcggcc gtg
//
LOCUS       DATMFS010000576         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_228487, whole genome shotgun sequence.
ACCESSION   DATMFS010000576 DATMFS010000000
VERSION     DATMFS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228487"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNQ33_10950"
     CDS             <1..508
                     /locus_tag="VNQ33_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98669.1"
                     /translation="SDPSTKMGLASLAFGAVYLALAAWRDRRGDARMATAALAPAVIV
                     LLYADGLLVGHIGWVGSGLLSMVVGSAIVGVGLRGGRRASSWIGVFLATVGLGSMVSE
                     GLRESPRAVGAVLTLLGVAVALAVGVADGRQTRRDAAVPASPPPGPEPGSEPDRPQPP
                     PSPWSQTF"
     gene            547..1341
                     /locus_tag="VNQ33_10955"
     CDS             547..1341
                     /locus_tag="VNQ33_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98670.1"
                     /translation="MTSRQTTQELGDRVHRRFAALAGAGGIANSCALSAMARWVLRRQ
                     PKRILEVGAGIGTTTAAITEAIDRSGATDVAHVAVERIPYCVEQLHANLGARMDAVTL
                     VNWPSEAPESTMPFDLVVIDGLGPKSATERFGPERLEAETVFCVQHLAPRAVVIVENR
                     REAQRQTIEGAARPGWVSAHVQPWDGSPGYHLYLFDPTPAERAQMALRRAWFAAWFPE
                     GPRFARRAYRKVTGRRLERRSLAATGPDYSHDGGDAVEDPGTSGTD"
     gene            complement(1358..2023)
                     /locus_tag="VNQ33_10960"
     CDS             complement(1358..2023)
                     /locus_tag="VNQ33_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWJ98671.1"
                     /translation="MDSLERVHDDVVSCRACPRLVAWREEVGREKRAAYRDKEYWARP
                     VPGFGDSRAGLVVVGLAPAAHGANRTGRMFTGDRSGDFLYAALHRAGFANQPESVSID
                     DGLELDGAWITAPVRCAPPANKPTIDERDACRPFLDRELALLEHASVFLVLGQFGYQG
                     LARYLGLRPRPPFGHGAEVPLPDGRTVLCSYHVSQQNTFTGKLTPEMLDAVLARANEL
                     LGR"
     gene            2118..>2335
                     /locus_tag="VNQ33_10965"
     CDS             2118..>2335
                     /locus_tag="VNQ33_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ98672.1"
                     /translation="MAATSRSKKVPAPPTSTLLLLVRHGQTPTTGATLPGRAKGLHLA
                     EAGRIQAGAVAARIAGLRDVSAVYASPL"
BASE COUNT          292 a          913 c          834 g          296 t
ORIGIN      
        1 ctccgacccg agcacgaaga tggggctggc atcgttggcc ttcggcgccg tgtacctcgc
       61 gctcgcggcc tggcgggacc ggaggggcga tgcccgcatg gccaccgccg cgctcgcgcc
      121 ggcggtcatc gtcctgctct acgccgacgg cctgctggtc ggccacatcg ggtgggtggg
      181 cagcgggctg ctcagcatgg tcgtcggcag cgccatcgtc ggcgtcggcc tgcgcggcgg
      241 ccggcgggcc agctcctgga tcggggtctt cctggccacg gtcggcctgg gctcgatggt
      301 ctcggagggc ctgcgggagt ccccgcgcgc cgtcggtgcg gtcctcaccc tgctcggcgt
      361 cgccgtcgcg ctcgccgtcg gcgtggccga cggccgacag acccgtcgcg acgcggccgt
      421 ccccgccagc ccgccgccag gaccggaacc gggatccgag ccggaccggc cgcagccgcc
      481 gccgtcgccc tggtcccaga cgttctgatt cctcgttccc tcgggcggac ggtagggtca
      541 cccgtcgtga cgagccgcca gaccacccag gagctcggcg accgggtgca ccggcgcttc
      601 gcggcgctcg cgggggcggg cggcatcgcc aactcctgcg ccctgagcgc catggcccgg
      661 tgggtgctgc ggcgccagcc gaagcggatc ctcgaggtcg gcgccggcat cggcaccacc
      721 accgcggcca tcaccgaggc gatcgaccgg tctggcgcca cggacgtcgc ccacgtcgcc
      781 gtcgagcgga tcccctactg cgtcgagcag ctgcatgcga acctcggcgc ccggatggac
      841 gccgtcacgc tggtcaactg gccgagcgag gcgcccgagt ccaccatgcc cttcgacctc
      901 gtggtcatcg acgggctggg acccaagtcc gccacggagc ggttcggtcc agagcgcctg
      961 gaggcggaga ccgtcttctg cgtccagcac ctcgctcccc gggctgtcgt catcgtggag
     1021 aaccgccgcg aagcccagcg ccagacgatc gagggcgccg cccggcccgg ctgggtcagc
     1081 gcccacgtcc agccgtggga cggctccccc ggctaccacc tgtacctgtt cgatccgacc
     1141 cccgccgagc gggcgcagat ggcgctgcgc cgagcgtggt tcgccgcctg gttcccggag
     1201 gggccgcggt tcgcccgccg cgcctaccgg aaggtcacgg gcaggcgcct ggagcggcgg
     1261 agcctcgccg ccaccggccc cgactactcg cacgacgggg gcgacgccgt cgaggacccg
     1321 ggcacgagcg ggaccgactg accctcgggt gggtggctca gcggccgagc agctcgttcg
     1381 cccgggcgag gaccgcgtcg agcatctcgg gcgtgagctt gccggtgaac gtgttctgct
     1441 ggctcacgtg gtagctgcac aggaccgtgc ggccgtcggg cagcggcacc tcggcaccgt
     1501 ggccgaacgg cggcctcggg cgcaggccga ggtaccgggc cagcccctgg tagccgaact
     1561 gcccgaggac gaggaagacc gacgcgtgct cgagcagcgc cagctcccgg tcgaggaacg
     1621 gccggcacgc gtcgcgctcg tcgatggtcg gcttgttcgc cggcggcgcg caccgcaccg
     1681 gcgcggtgat ccaggcgccg tcgagctcga ggccgtcgtc gatgctcacg ctctcgggct
     1741 ggttcgcgaa ccccgcccgg tggagcgcgg cgtacaagaa gtcgcccgag cggtcaccgg
     1801 tgaacatccg accggtccgg ttcgctccgt gcgctgcggg tgcgagaccg accaccacca
     1861 agcccgcacg ggagtcgccg aagcccggca ccggccgcgc ccagtactcc ttgtcgcggt
     1921 acgccgcccg cttctcccgc ccgacctcct cccgccaggc gaccaggcgg gggcaggcgc
     1981 gacacgacac gacgtcgtcg tgcacccgct ccaggctgtc cacctcggcg acggtaccgc
     2041 cctcggccgc gacctcggaa cccccgccgg accacggacc cgcaccggct ccgacggccg
     2101 gtagcgtgag ccgaccgatg gctgccacct cccgctccaa gaaggttcct gccccgccca
     2161 ccagcacgct cctgctgctg gtgcgccacg ggcagacccc caccaccggc gccacgctcc
     2221 caggccgggc caagggcctg cacctggcgg aagcggggcg catccaagcc ggggccgtcg
     2281 ccgcccgcat cgccgggctg cgcgacgtct cggccgtcta cgcgtcgccg ctgga
//
LOCUS       DATMFS010000577         2429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_228543, whole genome shotgun sequence.
ACCESSION   DATMFS010000577 DATMFS010000000
VERSION     DATMFS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228543"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VNQ33_10970"
     CDS             complement(<1..638)
                     /locus_tag="VNQ33_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98673.1"
                     /translation="MGDLVDRSEVGVAALAHANYVEFGRETARWSGLRGEVLERDGAV
                     LWASASDFPVSMNGVVRADPSASAADVLDLADAWFGERERGYSLTTTGLFDDDDLVAA
                     AEARGIVRLMDVPAMVCDARLDDRAAPDGVTVRLASSADDVAAFVAVNDAAYQTLGMP
                     EGVIAAAITVPERVLARQVRTVIAWEGDTPLACAQVLLSHGVAGIYYVGTAEA"
     gene            840..1229
                     /locus_tag="VNQ33_10975"
     CDS             840..1229
                     /locus_tag="VNQ33_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWJ98674.1"
                     /translation="MNQFCSNCGSQIADSDKFCKSCGIDLHPAPKPDQPAAAPAKGGG
                     SNTGAIVAVIVVLVVILGVIGFLLINDTDDDTPGTTTTTATTQPDNPTTTQAPPTTEA
                     PTTAPPTTEAPTTAPPTTEAPTTTTAP"
     gene            complement(1471..1557)
                     /locus_tag="VNQ33_10980"
     tRNA            complement(1471..1557)
                     /locus_tag="VNQ33_10980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1521..1523),aa:Leu,seq:cag)
     gene            1667..2326
                     /locus_tag="VNQ33_10985"
     CDS             1667..2326
                     /locus_tag="VNQ33_10985"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HWJ98675.1"
                     /translation="MALKGFERRLERMVEGTFSRLFRSTIRPVELGRRLVREMDDNRS
                     VDVRGRTLVPNAYSVELSEIDLEQFSEVADGLERELAEAAREHARDEGYVFMGPVSVH
                     LELSEKQHAGSFQIVGRMREGKGGAGAGALLLPTGERLQLSEKVVTIGRKPESTIQLG
                     DPNVSRNHAEIRPHGNGWMLVDLGSTNGSRVNGNRVSSHELHEGDEIAFGNTVLRFEA
                     S"
BASE COUNT          381 a          892 c          849 g          307 t
ORIGIN      
        1 gcctcggcgg tgccgacgta gtagatgccg gcgaccccgt ggctgaggag cacctgggcg
       61 caggcgagcg gggtgtcgcc ctcccaggcg atgacggtgc gcacctgtcg ggcgagcact
      121 cgctcgggca cggtgatcgc agcggcgatc acgccctcgg gcatcccgag cgtctggtac
      181 gccgcgtcgt tcaccgccac gaaggcggcg acgtcgtcgg ccgaggaggc gaggcgcacg
      241 gtcacgccgt caggcgcggc gcggtcgtcg agccgggcgt cgcacaccat ggccggcaca
      301 tccatcaggc gcacgatccc tcgggcttcg gcggcggcga cgaggtcgtc gtcatcgaag
      361 agccccgtgg tcgtcagcga gtagccccgt tcgcgctcgc cgaaccaggc gtcagcgagg
      421 tcgagcacgt cggctgcgga agccgaaggg tcggcgcgca cgacgccgtt catgctcacg
      481 gggaagtcgg aggccgacgc ccagagcacg gcgccgtcgc gctcgaggac ctcgccgcgg
      541 agaccggacc accgggcggt ctcccggccg aactccacgt agttggcgtg ggcgagcgcg
      601 gcgacgccca cctcgctccg gtccaccaga tcgcccatcg ccagcccccg tcgtcggtcc
      661 tgtcccgatc atgaccggtg gcagcgggcc gaggcgctcg gatttgccgg ccggaggctg
      721 gctgcgggga cgtgggcacg gcgtccgcgc cgaggatgca gcggcgctcc ggtccccgac
      781 ggtcccgttc acggccccta ggctcgatcc gggtcgttca gatgcgggag ggcgccaccg
      841 tgaaccagtt ctgctccaac tgcggatcgc agatcgccga cagcgacaag ttctgcaaga
      901 gctgcggcat cgacctgcat ccagctccga agccggacca gcccgcggcg gctccggcca
      961 agggcggtgg ctccaacacc ggggcgatcg tcgcggtgat cgtggtgctg gtggtcatcc
     1021 tcggcgtgat cggcttcttg ctgatcaacg acaccgacga cgacaccccc ggcaccacga
     1081 cgacgacggc cacgacccag ccggacaacc cgacgacgac ccaggccccg cccaccacgg
     1141 aggccccgac cacggcccca cccacgaccg aggccccgac cacggcaccc cccaccaccg
     1201 aggccccgac cacgaccact gccccctgac gcaggcgggt cacccggccg gcctgctccc
     1261 gtggcccggc ggcggcgagg gctgcgggag ctgatctcag gcgctggcca agcgctggca
     1321 gtacgccgtc tcgtgagacc cagaccgttc cgtgggcgag ccagcaggga atccgaccga
     1381 ggggtgaaac ggaaccggcc acccccgagg aggtggccgt ttctctggct gtttcgcaaa
     1441 cccggagacc cggatccgcg aacaaccccg tggtgcgcga ggagggactt gaaccctcac
     1501 gtccgttagg acactggaac ctgaatccag cgcgtctgcc aattccgcca ctcgcgcgag
     1561 cagcggggag accgtagcgc accgggcagg atcgacccca gcccgttccg cctgctgccg
     1621 gtcggggccc acgggcgcgg ggcctgtccc ggtaacctgc tcggccgtgg cgctcaaggg
     1681 cttcgaacga cgcttggaac ggatggtcga gggcaccttc tcgcgcctgt tccgctccac
     1741 catccgtccc gtggagctcg gccgccgcct ggtccgggag atggacgaca accgatcggt
     1801 cgacgtccgg ggccgcacgc tcgtccccaa cgcctacagc gtcgagctct ccgagatcga
     1861 cctcgagcag ttcagcgagg tggccgacgg cctcgagcgc gagctggccg aagccgcccg
     1921 ggagcacgcc cgcgacgagg ggtacgtctt catgggcccg gtgtcggtgc acctcgagct
     1981 gtcggagaag cagcacgccg ggtcgttcca gatcgtgggc cgcatgcgcg agggaaaggg
     2041 cggcgccggt gccggcgcgc tgctccttcc caccggcgag cggctccagc tgagcgagaa
     2101 ggtcgtcacc atcggccgca agcccgagtc cacgatccag ctcggtgacc cgaacgtgag
     2161 ccggaaccac gccgagatcc ggccccacgg caacgggtgg atgttggtgg acctcgggtc
     2221 caccaacggg tcccgcgtca acggcaaccg ggtcagctcc cacgagctgc acgagggcga
     2281 cgagatcgcg ttcggcaaca cggtcctgcg gttcgaggcg tcctgagcgc tcgccgggcg
     2341 gctcgttggc ccgaccgcct cgcacccggc gcgccggacc gcgaaccggc cgaccggtag
     2401 gctcgaccgc tcatggacga gcagctgca
//
LOCUS       DATMFS010000578         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_180062, whole genome shotgun sequence.
ACCESSION   DATMFS010000578 DATMFS010000000
VERSION     DATMFS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180062"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNQ33_10990"
     CDS             complement(<1..291)
                     /locus_tag="VNQ33_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ98676.1"
                     /translation="MVDGQHFIGGAWRPALSGATDGIVNPATGEVITAVPSGDGADVD
                     VAVAAAAAAFGSWGKTTPRERSERLLALADAVEQDLPELKRLEMENVGKPASI"
     gene            490..1674
                     /locus_tag="VNQ33_10995"
     CDS             490..1674
                     /locus_tag="VNQ33_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98677.1"
                     /translation="MTIADAAEAAVPTEQRPVIDIDDVSKVFADFTAVDHADFTIAEG
                     EFFSMLGPSGCGKTTTLRMIAGFETPTSGAIRLEGRDVSNVPPHKRDVNTVFQQYALF
                     PHMNVWDNVAYGPRAKKLPKEEIAKRVDELLGVVKLADFAKRKPSQLSGGQQQRIALA
                     RALVNYPKALLLDEPLGALDLKLRQSMQLELKRIQREVGITFIFVTHDQEEALTMSDR
                     IAVMNQGRVEQIGTPREIYDRPETVFVAGFIGQANLWPAVVKANEGTTTTIEVAGRSL
                     KVADDASGLRPGEHGTFMVRPERMYVEPQLSDPDRWTLDAVVTDLVFQGPVVRYELRL
                     PDGSAAVAHVPARRDGLPIPGDTVQASWSDEASVLLPGTPDQAPAAETTPVIAPDPDP
                     ET"
     gene            1709..2935
                     /locus_tag="VNQ33_11000"
     CDS             1709..2935
                     /locus_tag="VNQ33_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWJ98678.1"
                     /translation="MPDQPTDPQLAAAIEMARRMSRRRFLTSMGLGVGAAALGPSFLA
                     ACGSSSDSGGGSEGAAPGNGDTKLTISNWDAYIDEDANGNVTAAGTTIGDFQKATGIK
                     VTYNKDFNDNDEYFNKIYSPVLGKGKRIAADITVPTYWMAARLVGLGWLEELPLDDIP
                     NHKNMVDSYLDLPWDPGAEHFMPWQAGISGIAWNPKLTGGDLTSVNDLFEPKLKGKVT
                     FLTEMRDSVGLVMFGQGNDPADADVATIDKALDKLEEETNNGQILKFTGNEYLRSLEN
                     GDVAACLAWSGDIAQLDPDLGIQFAIPEEGGMQWFDAMTVPKGAANLPAAAEWMNFVY
                     DPENAARITEFVQYISPVKGVQEVLEAKGGEAAELASNPLIFPDEETLARVVTFGELS
                     VEDEIEVQKRFNDITG"
     gene            3005..3835
                     /locus_tag="VNQ33_11005"
     CDS             3005..3835
                     /locus_tag="VNQ33_11005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98679.1"
                     /translation="MLWLILFFLVPMWTLLRIALSTKPNAYLPEYEFTWEWSNFSSAV
                     DRFQPELVRSFSYAAAATVLCIAIGYPLAYFLAFKAGKYRTVLLGLVMVPFFISFLIR
                     TIAWQTILGQEGPALWFLQTFRLVTVTDALGITSSGAFLNTPQAVILGLTYNFVPFMI
                     LPIYVSLEKIEPALISAGQDLYSTPRQAFRRIVLPLSLPGVFAGTLLTFIPSAGDYIN
                     AELLGNVKTSMIGSQISKLFLKESNYPEAAALSFIFMAIITVGVLVYARLLGTDELSA
                     "
     gene            3832..4680
                     /locus_tag="VNQ33_11010"
     CDS             3832..4680
                     /locus_tag="VNQ33_11010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98680.1"
                     /translation="MTASIASDGRWPEPVKPPKERRPLRSRWLAIWSALVYIWLFAPI
                     VVVVAFSFNHPAGKFNTSWNEFTFDNWLHPFAKQDYTKALIVSLKVAAVSCVIATVLG
                     GLMALAISRYEFRGKGFVNIVLVLPLTTPEIVMGSSLFTLFFARDLDFGFTTVVIAHI
                     LFCVSFVALTIQARVRGFDWSLEDAALDLGSTPLRTFWTITFPLILPGIGAAFLLSFA
                     LSIDDYIITSFVAGDHTTTFPMRIFNANKTEIPPQVNVIATAILVVTGTLMALGARRA
                     IKQARA"
     gene            4767..>5163
                     /locus_tag="VNQ33_11015"
     CDS             4767..>5163
                     /locus_tag="VNQ33_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ98681.1"
                     /translation="MSTPALQEHPVNASPTPAELQSLARDHLWGHFSKLTPEDHGNVT
                     IIDHGDGCYVWDTNGKRYLDGLAGLFTVQAGHGRPSIAKAMAEQAEKLAYFPVWTYAH
                     PTAVELAGRLAALAPGDLNRAFFTTGGGEA"
BASE COUNT          877 a         1910 c         1567 g          809 t
ORIGIN      
        1 gatcgaggcc ggcttgccga cgttctccat ctccaaccgc ttcagctccg gcaggtcctg
       61 ctcgaccgca tcggccagcg cgagcagcct ctcgctgcgc tcgcgcggcg tcgtcttccc
      121 ccaggagccg aacgcggccg ccgccgccgc gaccgcgacg tcgacgtcgg cgccgtcgcc
      181 cgagggcacc gcggtgatca cctcgccggt cgccgggttc acgatgccgt cggtggcccc
      241 gctcagcgct gggcgccacg ccccgccgat gaagtgctgg ccgtcaacca tgggcggtgc
      301 ctccgagctc gatccgacgg cgccatcctc cgtggtcagc gcggagatgg caagggattc
      361 cgtcgcgaaa cacaatggaa acgattgatt ccttgccaag cgggggcatc cgtgggacgc
      421 tgcgagcgca tccccaaagc atgacttgcg ccacagggga cgtggaccgc ccgcacaacg
      481 aggacgccca tgacgatcgc cgacgccgcc gaggctgccg ttccgaccga gcagaggccg
      541 gtcatcgaca tcgacgacgt gtcgaaggtg ttcgccgact tcaccgccgt cgaccacgcc
      601 gacttcacca tcgccgaggg cgagttcttc tcgatgctcg gcccatcggg gtgcggcaag
      661 accaccacgc tgcgcatgat cgccgggttc gagaccccga catcgggggc gatccgcctc
      721 gagggccgcg acgtctcgaa cgtgccgccg cacaagcgcg acgtgaacac cgtgttccag
      781 cagtacgccc tgttccccca catgaacgtg tgggacaacg tggcctacgg gccccgcgcc
      841 aagaagctcc cgaaggagga gatcgccaag cgggtcgacg agctcctcgg cgtggtcaag
      901 ctggccgact tcgccaagcg caagcccagc cagctgtccg gcggccagca gcagcgcatc
      961 gctttggccc gtgccctcgt caactacccg aaggcgctcc tgctcgacga gccgctcggc
     1021 gcgttggacc tcaagctgcg gcagagcatg cagctcgagc tgaagcgcat ccagcgcgag
     1081 gtcggcatca ccttcatctt cgtcacccac gatcaggagg aagcgctcac catgagcgac
     1141 cggatcgcgg tcatgaacca gggccgcgtc gagcagatcg gcaccccccg cgagatctac
     1201 gaccgccccg agacggtgtt cgtcgccggg ttcatcggcc aggccaacct ctggcctgcg
     1261 gtcgtgaagg ccaacgaggg gacgacgacc accatcgagg tcgcaggccg gtctctgaag
     1321 gtcgccgacg acgcgagcgg gctccgaccc ggcgagcacg gcaccttcat ggtgcgtccc
     1381 gagcgcatgt acgtcgagcc ccagctctcc gatcccgacc ggtggaccct cgatgccgtc
     1441 gtcaccgacc tcgtgttcca gggccccgtg gttcgctacg agttgcgcct gcccgacggt
     1501 tccgccgcgg tcgcccacgt acccgcccgg cgcgacggcc tgcccatccc cggcgacacg
     1561 gtccaagcct cctggtccga cgaggcgtcc gtcctgttgc caggaacccc cgaccaagcc
     1621 cccgccgccg agaccacgcc ggtgatcgca ccggatcccg accccgagac ctgacccacc
     1681 cgtccgcaag ttcgcaggag cgtccgccat gcccgatcaa cccaccgatc cccagctcgc
     1741 cgccgccatc gagatggcac gccgcatgtc gcggcgccgc ttcctcacgt cgatgggcct
     1801 cggcgtcggt gccgccgccc tcggccccag cttcctcgcc gcctgcggca gcagcagcga
     1861 cagcggcggc ggatccgagg gagccgcacc gggcaacggc gacacgaagc tcacgatcag
     1921 caactgggac gcgtacatcg acgaggacgc caacggcaac gtcaccgccg cgggcacgac
     1981 catcggcgac ttccagaagg ccaccggcat caaggtgacg tacaacaagg acttcaacga
     2041 caacgacgag tacttcaaca agatctactc gcccgtcctc ggcaagggga agcggatcgc
     2101 cgcggacatc accgtcccca cctactggat ggcggcccgc ctcgtgggcc tcgggtggct
     2161 cgaggagctg ccgctcgacg acatcccgaa ccacaagaac atggtcgact cctacctcga
     2221 cctgccgtgg gatccagggg ccgagcactt catgccgtgg caggcgggca tcagcggcat
     2281 cgcctggaac ccgaagctga ccggcggcga cctcacgagc gtcaacgacc tgttcgagcc
     2341 gaagctcaag ggcaaggtca ccttcctcac cgagatgcgc gactcggtcg gcctcgtgat
     2401 gttcggccaa ggcaacgatc ccgcggacgc cgacgtcgcc accatcgaca aggcgctcga
     2461 caagctcgag gaggagacca acaacggcca gatcctgaag ttcaccggca acgagtacct
     2521 ccggtcgctg gagaacggcg acgtcgccgc gtgcttggcg tggtccggtg acatcgccca
     2581 gctcgacccg gacctcggca tccagttcgc catccccgaa gagggcggga tgcagtggtt
     2641 cgacgccatg accgtcccga agggcgccgc caacctcccc gcggcagccg agtggatgaa
     2701 cttcgtgtac gacccggaga acgcggcccg catcaccgag ttcgtccagt acatatcgcc
     2761 ggtcaagggg gtccaggagg tcctcgaggc caagggcggc gaggcagccg agctcgcgag
     2821 caacccgctg atcttccccg acgaggagac gctcgctcgc gtggtcacct tcggcgagct
     2881 gtcggtggaa gacgagatcg aggtccagaa gcgcttcaac gacatcaccg gctgatccgc
     2941 cgtggctgcc gaggactccc tgcgaagagg ccggagagcg ccgtacgggc tgctgagccc
     3001 cgggatgctc tggctgatcc tgttcttcct cgtcccgatg tggaccctgc tgcggatcgc
     3061 cctgtcgacg aagccgaacg cctacctccc cgagtacgag ttcacgtggg agtggtcgaa
     3121 cttctccagc gcggtcgacc ggttccagcc tgagctcgtc cgctccttct cctacgcggc
     3181 cgcggccacc gtcttgtgca tcgccatcgg ctacccgctg gcgtacttcc tcgcgttcaa
     3241 ggcggggaag taccgcaccg tgctcctcgg cctggtgatg gtgccgttct tcatctcgtt
     3301 cctgatccgc accatcgcct ggcagaccat cctcggccag gaggggccgg cgctctggtt
     3361 cctccagacg ttccggctcg tgaccgtcac cgacgccctg gggatcacca gcagcggcgc
     3421 gttcctcaac acgccgcagg cggtcatctt gggcctgacc tacaacttcg tcccgttcat
     3481 gatcctgccg atctacgtca gcctcgagaa gatcgagccc gcgctcatca gcgccggcca
     3541 ggacctctac tcgaccccgc gccaggcgtt ccgcaggatc gtgctgccgc tctcgctccc
     3601 cggcgtgttc gcgggcacgc tcctcacctt catcccgtct gccggcgact acatcaacgc
     3661 ggagctgctg ggcaacgtga agaccagcat gatcgggtcg cagatctcga agctcttctt
     3721 gaaggagagc aactatccgg aggcggcggc gctgtcgttc atcttcatgg cgatcatcac
     3781 cgtgggcgtg ctggtgtacg cccggctcct cggcaccgac gagctgtccg catgaccgcg
     3841 tcgatcgcgt ccgacggacg gtggcccgag ccggtgaagc cgccgaagga gcgccgcccg
     3901 ctgcgctcac ggtggctggc catctggtcg gccctcgtct acatctggct gttcgcgccg
     3961 atcgtcgtcg tggtcgcgtt ctcgttcaac caccccgccg gcaagttcaa cacctcgtgg
     4021 aacgagttca cgttcgacaa ctggctgcac cccttcgcca agcaggacta cacgaaagcg
     4081 ctgatcgtga gcctcaaggt ggcggcggtg tcgtgcgtca tcgcgaccgt cttgggcggg
     4141 ctcatggccc ttgccatctc ccgctacgag ttccggggga agggcttcgt caacatcgtc
     4201 ttggtcctgc cgctgaccac gcccgagatc gtgatgggct cgtcgctgtt cacgctcttc
     4261 ttcgcccggg acctggactt cggcttcacg accgtcgtga tcgcccacat cctgttctgt
     4321 gtcagcttcg tggcgctcac catccaggcg agggtccgcg gcttcgactg gtccttggag
     4381 gacgcggccc tcgacctggg ttccacgccg ctgcgcacgt tctggaccat caccttcccg
     4441 ctgatcctcc ccgggatcgg cgcggcgttc ctcctctcgt tcgcgctctc gatcgacgac
     4501 tacatcatca cgtcgttcgt cgccggcgac cacacgacga cgttcccgat gcggatcttc
     4561 aacgccaaca agaccgagat cccaccgcag gtcaacgtca tcgcgacggc gatcctcgtg
     4621 gtgaccggca cgctgatggc gctcggcgcc cggcgagcca tcaagcaggc acgagcctga
     4681 gcacaaccaa gacaactgat tccatcgtca aaggcacggt tcacaacagg atcccttgct
     4741 caagccggga gcatcaacga gaatcgatgt cgacacccgc cctccaggag caccccgtga
     4801 acgcctcccc cacccccgcc gaactccagt ccctcgcccg ggaccacctg tgggggcact
     4861 tctcgaagct gacccccgaa gaccacggca acgtcaccat catcgaccac ggcgacggct
     4921 gctacgtgtg ggacaccaac ggcaagcgct acctcgacgg gctcgccggc ctcttcaccg
     4981 tccaggccgg ccacggccgc ccgtccatcg ccaaggccat ggccgagcag gcggagaagc
     5041 tcgcctactt ccccgtctgg acctacgccc accccaccgc ggtcgagctc gccggacgcc
     5101 tcgccgcact ggcccccggc gacttgaacc gggccttctt caccacgggc ggcggcgaag
     5161 ccg
//
LOCUS       DATMFS010000579         4745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_43998, whole genome shotgun sequence.
ACCESSION   DATMFS010000579 DATMFS010000000
VERSION     DATMFS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43998"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1013)
                     /locus_tag="VNQ33_11020"
     CDS             complement(321..1013)
                     /locus_tag="VNQ33_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWJ98682.1"
                     /translation="MADLNVDVVFLDAGGVLVGPSRDVIRDRFAAAGFSIDPDLLFEA
                     HYRGVQAIDIAEAAPEVFDDYHRAYAAHIGVDEVSAPSASAVLQGLWELSGLWGEILP
                     WAADGLAAIAATGVPIVIVSNADGTVEELLTGTGLLQVGPGPGTEVAAVVDSGALGIA
                     KPDPAIFHHALGLVGATPDRAIHVGDAYQYDVRGARAAGVRPVLLDPLGIRTEADCDR
                     VASLVEVVALLH"
     gene            complement(1082..1741)
                     /locus_tag="VNQ33_11025"
     CDS             complement(1082..1741)
                     /locus_tag="VNQ33_11025"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HWJ98683.1"
                     /translation="MTDLVRTLIVCGLISGTQPFTILGMFLVLGGEQGTRNVWWYLFG
                     CFSIQFVIVVIVGSLVEGTVAADSTGGHSLIGLRILAGLVLVGLGAWLRRPPRREAPD
                     TPKAFDRLDNVGAGASYIGGILIADYQGSVLAAGALATAQVSSADQLVGWAVYCLFAT
                     GIPVVAAVATIRSARAEARLHRTIDWVVTNRRTLASWICLVGGLLLFGDGLASWVQAG
                     S"
     gene            complement(1751..3427)
                     /gene="ettA"
                     /locus_tag="VNQ33_11030"
     CDS             complement(1751..3427)
                     /gene="ettA"
                     /locus_tag="VNQ33_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWJ98684.1"
                     /translation="MAAQYIYTMHKLTRFYPPDREVLKDISLSFYPGAKIGVLGANGA
                     GKSSLLKVMAGTDDGFTGEARLTQGFTVGMLEQEPHLDESKSVKDNVMDGVAEVAGLL
                     TAYDACLAKYADPDADYEKIGEEQARLEAEIAAKGANDLDRKIEIAMDALRLPPGDAD
                     VTNLSGGEKRRVALCRLLLSAPDLLLLDEPTNHLDAESVAWLERFLRDYAGTIVAITH
                     DRYFLDNVAGWILELDRGRGIPFEGNYSGWLEQKSARLAQEEKQASARQRTLNQELEW
                     VRMSPKARQSKGKARLAAYDKLLEESKQAEGRDAKLQIFIPSGDRLGDQVIEVESLSK
                     GFDDKLLIEDLSFSLPKAGIVGIIGPNGAGKTTLFKMLTGQEQPDSGSITIGSTVNLA
                     YVDQSRDSLDDSKTVYEEITGGKDDLVVGGREIHGRAYVASFNFTGSDQQKTVGVLSG
                     GERNRVHLAKLLKEGGNVLLLDEPTNDLDVDTLRALEDALEAYAGCAVVISHDRWFLD
                     RIATHMLAFEGDSQVRWFEGNFSEYEAFRRKELGAEADQPHRIAYKRLTR"
     gene            3593..>4745
                     /locus_tag="VNQ33_11035"
     CDS             3593..>4745
                     /locus_tag="VNQ33_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWJ98685.1"
                     /translation="MAENHSNDALGPNAWLVDEMYEQYLENPAAVSESWRDFFADYQR
                     DSDVTASTLDKSLPATGRTAETGQLPVVKAGAPAPVAAAAPAPAAPAAPKTTGIAPDG
                     TEEVPGEPLRGAAARIVTNMEASLAVPTATSFREVPAKLLEVNRKIINGYLGRTRGGK
                     VSFTHLIGYAIVRAVADKVPAMNASYLEGPDGKPRIVRNPTVGLGLAVDVEKKDGSRT
                     LLVPVIREADTLDFRGFHGAYEDVIRKVRSNKLTPDDFAGATITLTNPGTIGTVQSVP
                     RLMPGQGVIVGVGRLDFPAAFGGSDPNALAEMGLSKVMTVTSTYDHRIIQGAESGLFL
                     KRVQELLMGADGFYDGVFHSLGVPYEAVQWRTDDNPVDREQSRLEKQMNV"
BASE COUNT          696 a         1622 c         1739 g          688 t
ORIGIN      
        1 tggtcttgtc gtcgggggcc gccatcgcct gccgcctgtc agcggacccg acatcgtcca
       61 cagcagtggc agccggggcg cctcgtcctc gccgcgagcc cgtccctgtc gacggactgc
      121 cttcggggcc gcgccgacga caggctccga gcggcggcgg tccttgtggt cgttctgccc
      181 cgaggagggg ggtcagcccc gagcggggtc atctgacgac acgctcagta cggcagcgct
      241 cgcggtggcc gggtgctctc gacggcgctg ctcggcgacc gtcgggccag cgcgggctgc
      301 cgccggggga gccgggcccg tcagtgcagc agggccacga cctcgaccag cgacgccacg
      361 cggtcgcagt cggcctcggt gcggatgccg agcggatcga gcagcaccgg gcggacgccg
      421 gcagcccggg ctccccggac gtcgtactgg taggcatcgc ccacgtggat ggcccggtcc
      481 ggcgtcgcac cgaccaagcc cagggcgtgg tggaagatgg cgggatcggg cttggcgatg
      541 ccgagtgccc ccgagtcgac gacggcggcg acctcggtgc cgggccccgg gccgacctgg
      601 agcaggcccg tgccggtcag gagctcttcg acggtgccgt cggcgttgga cacgatcacg
      661 atcggcacgc cggtggcggc gatcgcggcc aggccgtcgg ccgcccaggg gaggatctcc
      721 ccccacagtc cggagagctc ccacaggccc tggagcaccg cggaggcgga gggcgccgag
      781 acctcgtcga ccccgatgtg ggcggcgtag gcgcggtggt agtcgtcgaa gacctcgggg
      841 gccgcttcgg cgatgtcgat cgcctggacg ccccggtagt gggcttcgaa cagcaggtcc
      901 gggtcgatcg agaagccagc agcggcgaac cggtcgcgga tcacgtcgcg gctggggccg
      961 acgaggacgc caccggcatc gaggaacacc acatcgacgt tcagatcggc catgccttga
     1021 ccgtagacgg cggcggtgct gtggccggat gggttcagca gggagcggtg cagccgtcgg
     1081 gctagctgcc ggcttggacc cagctcgcga ggccgtcgcc gaagagcagc aggccgccga
     1141 ccaggcagat ccagctcgcc agcgtccggc ggttggtgac cacccagtcg atggtgcggt
     1201 gcagccgggc ctcggcccga gcagagcgga tggtggcgac ggcggccacg accgggatgc
     1261 ccgtcgcgaa gaggcagtag accgcccagc cgacgagctg atcggccgag ctgacctggg
     1321 cggtggcgag cgcgccggcg gcgaggaccg agccctggta gtcggcgatg aggatgccgc
     1381 cgatgtacga ggcgccggcg ccgacgttgt cgagccggtc gaaggccttc ggcgtgtcgg
     1441 gcgcctccct cctcggtgga cgccggagcc aggcgccgag cccgaccagc acgaggccgg
     1501 cgaggatccg cagcccgatc agcgagtggc caccggtcga gtccgcggcg accgtcccct
     1561 cgacgagcga gccgacgatg acgacgatca cgaactggat gctgaagcag ccgaacaggt
     1621 accaccacac gttgcgggtc ccctgctcgc cgccgagcac caggaacatg ccgaggatcg
     1681 tgaacggctg cgtcccgctg atgaggccgc acacgatcag cgtgcgaacc aggtcggtca
     1741 tcgaggcgcc ctaccgggtc agcctcttgt aggcgatgcg gtggggctgg tcggcctcgg
     1801 cgccgagctc cttgcggcgg aaggcctcgt actcggagaa gttgccctcg aaccagcgga
     1861 cctgggagtc gccctcgaac gccagcatgt gcgtggcgat gcggtcgagg aaccagcggt
     1921 cgtgggagat gaccacggcg cagccggcgt aggcctcgag tgcgtcctcc agggcgcgca
     1981 gggtgtcgac gtcgaggtcg ttcgtgggct cgtcgaggag caggacgttg ccgccctcct
     2041 tgaggagctt ggcgaggtgc acccggttgc gctcgccgcc cgacagcacg ccgacggtct
     2101 tctgctggtc cgagccggtg aagttgaacg atgccacgta ggcccggccg tggatctccc
     2161 ggccgccgac cacgaggtcg tccttgccgc cggtgatctc ctcgtagacc gtcttcgagt
     2221 cgtcgaggct gtcgcggctc tggtcgacgt aggcgaggtt caccgtcgag ccgatggtga
     2281 tcgagcccga gtcgggctgc tcctggccgg tcagcatctt gaagagggtg gtcttgccgg
     2341 cgccgttcgg gccgatgatg ccgacgatgc cggccttcgg cagcgagaag gacaggtcct
     2401 cgatgaggag cttgtcgtcg aagcccttcg acaggctctc gacctcgatc acctggtcgc
     2461 cgaggcggtc gcccgacggg atgaagatct ggagcttggc gtcgcggccc tcggcctgct
     2521 tcgactcctc gaggagcttg tcgtaggcgg cgaggcgggc cttgcccttc gactggcggg
     2581 ccttgggcga catccgcacc cactcgagct cctggttgag ggtgcgctgg cgggccgatg
     2641 cctgcttctc ctcttgggcg aggcgggcgg acttctgctc cagccagccc gagtagttgc
     2701 cctcgaacgg gatgccccgg ccgcggtcga gctcgaggat ccagccggcc acgttgtcga
     2761 ggaagtagcg gtcgtgggtg atggccacga tggtgccggc gtagtcgcgc aagaagcgct
     2821 ccagccacgc caccgactcg gcgtcgaggt ggttggtggg ctcgtcgagc agcagcaggt
     2881 cgggggccga cagcagcagt cggcacagcg ccacgcggcg cttctcaccg ccggagaggt
     2941 tggtgacgtc ggcgtcgccc gggggcaggc gcagggcgtc catggcgatc tcgatcttgc
     3001 ggtcgaggtc gttggcgccc ttggcggcga tctcggcctc caggcgggcc tgctcctcgc
     3061 cgatcttctc gtagtcggcg tcggggtcgg cgtacttggc gaggcacgcg tcgtaggcgg
     3121 tgagcagccc ggccacctcg gccacgccgt ccatcacgtt gtccttgacg ctcttggact
     3181 cgtcgaggtg cggctcctgc tcgagcatcc cgacggtgaa gccctgggtg aggcgggcct
     3241 cgccggtgaa gccgtcgtcg gtgccggcca tgaccttgag cagcgacgac ttgccggcgc
     3301 cgttggcgcc gaggacgccg atcttggcgc ccgggtagaa ggacaggctg atgtccttca
     3361 gcacctcgcg gtccggcggg tagaaccggg tgagcttgtg catcgtgtag atgtactggg
     3421 ccgccatgcg agcggtacct cctggagacg ggggcgtcgg gctgccgacg ccagcgggcc
     3481 agggtagtgg gggagctgca cacgcccagc agggcgacgt gcgctcgccg tcgacggatg
     3541 ttcgcgctgg gggcggatga ggggttgggg cgcctcgtta ggatcgacgg cgatggctga
     3601 gaaccacagc aacgacgcac tcgggcccaa cgcgtggctc gtcgacgaga tgtacgagca
     3661 gtacctggag aacccggccg cggtcagcga gagctggcgg gacttcttcg cggactacca
     3721 gcgggactcc gacgtcaccg ccagcacgct cgacaagtcc ctcccggcga ccggccggac
     3781 ggctgagacc ggccagctcc cggtcgtgaa ggccggtgcg ccggctcccg tcgcggctgc
     3841 ggcgccggcg ccggccgcgc ccgccgcccc caagaccacc ggcatcgctc ccgacggcac
     3901 cgaggaggtg cccggcgagc cgctgcgagg cgccgcagcc cgcatcgtca ccaacatgga
     3961 ggcgagcctc gccgtcccga cggctacctc gttccgcgag gtgccggcca agctcctcga
     4021 ggtcaaccgc aagatcatca acgggtacct cggccggacc cgcggcggca aggtcagctt
     4081 cacccacctc atcggctacg ccatcgtccg cgccgtcgcc gacaaggtgc cggccatgaa
     4141 cgcctcgtac ctcgagggcc ccgacggcaa gccccgcatc gtgcgcaacc ccacggtcgg
     4201 cctcggcctc gcggtcgacg tggagaagaa ggacgggtcg cgcacgctgc tcgtcccggt
     4261 gatccgcgag gccgacacgc tggacttccg cggcttccac ggcgcctacg aggacgtcat
     4321 ccgcaaggtc cgctcgaaca agctgacacc cgacgacttc gccggtgcca ccatcacgct
     4381 caccaacccc ggcaccatcg gcaccgtcca gtcggtgccg cgcctcatgc ccggccaggg
     4441 cgtcatcgtc ggcgtcggcc ggctcgactt ccccgccgcg ttcggcggat cggaccccaa
     4501 cgccctcgcc gagatgggcc tgtcgaaggt catgacggtc acatcgacct acgaccaccg
     4561 catcatccag ggcgccgagt ccgggttgtt cctgaagcgg gtccaggagc tcctgatggg
     4621 ggccgacggc ttctacgacg gggtcttcca ctcgctgggc gtgccctacg aggcggtcca
     4681 gtggcggacc gacgacaacc cggtcgaccg ggagcagtcc cggctcgaga agcagatgaa
     4741 cgtgc
//
LOCUS       DATMFS010000580         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_323219, whole genome shotgun sequence.
ACCESSION   DATMFS010000580 DATMFS010000000
VERSION     DATMFS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323219"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VNQ33_11040"
     CDS             <1..1158
                     /locus_tag="VNQ33_11040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWJ98686.1"
                     /translation="DDATSGRRAAPFVDGVDLELMVSTFTDSFDLVQPSLALMDGRAT
                     VLDTAVADYADGASRTAWLTFAGALSMMAVLVALSLIVASTFERPLRRLIAGTRRVGG
                     GDLHLDPLPLSGPVEIEEATIAFNHMVANLRMLEGKLDALAETDFDDPRLDGELPGEL
                     GQALARSVEVLSSSIHDRASLQAKLVHQATHDTLTGIPNRAGGLAALEGALARARRQE
                     TTLAVAFLDLDGFKQANDTYGHQIGDAVLCEVATRLRHHARSGDSYARLGGDEFIVIA
                     ENIGSPANALTFARRIGTAIAEPIAAGEHLVEIGVSIGLALAPSGRASLVDLLACADH
                     AAYRAKRSKTGAELYDEVLDGGQGAVLGLMDPAPGPTEPVEITVDWVDLRS"
     gene            complement(1200..1811)
                     /locus_tag="VNQ33_11045"
     CDS             complement(1200..1811)
                     /locus_tag="VNQ33_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWJ98687.1"
                     /translation="MPTTTSEQARAAAAASLRELVTGNRRFVDGNPTSHVLTEADREA
                     LVAGQYPIAVLLGCVDSRVPPEVVLDQGAGDLLTVRTAGQSLAGVALGSIEFGVRVLG
                     LSLVVVLGHSGCGAVLAALSDDHPDGHLGELTGEVADRLIQVVGADPVRATGANLDAT
                     VTALRSMDTLATPAGHAPFVVGMMYDLATGEVTVTDDAGLLAG"
     gene            2012..>2399
                     /locus_tag="VNQ33_11050"
     CDS             2012..>2399
                     /locus_tag="VNQ33_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98688.1"
                     /translation="MRLHPSGFGLRTRLLVLVLVPAVLMAVVGGAAITRQAHAAANLA
                     EVHDEIGVLADLTELRRALLGARAPTELEVRAQAIGIDEDAAIRLLGVDQLEGGSLDE
                     VAERLRALPTEARPFTSAQLGALRAAP"
BASE COUNT          335 a          912 c          830 g          322 t
ORIGIN      
        1 gacgacgcca ccagcggccg gagggcggca ccgttcgtcg acggcgtcga cctcgagctg
       61 atggtctcga cgttcaccga cagcttcgac ctcgtccagc cctcgctggc gctcatggac
      121 ggtcgggcga cggtgctgga caccgccgtc gccgactacg ccgacggcgc ctcgcgaacc
      181 gcgtggctga ccttcgccgg cgcgctgtcg atgatggcgg tgctcgtcgc cctctccctg
      241 atcgtcgcat ccaccttcga gcggcccctg cggcggctca tcgccggcac ccgccgcgtc
      301 ggaggcggcg acctgcacct cgatcccctg cccctgagcg gtccggtcga gatcgaggag
      361 gcgacgatcg ccttcaacca catggtggcc aacctgcgca tgctcgaggg gaagctcgac
      421 gccctcgccg agaccgactt cgacgacccg cgcctcgacg gcgagctgcc cggcgagctc
      481 gggcaggccc tcgcccgctc cgtcgaggtg ctgtcgagct ccatccacga ccgggccagc
      541 ttgcaggcca agctggtcca ccaggcaacg cacgacacgc tcacgggcat ccccaaccgg
      601 gcgggcggcc tggcggcgct cgagggtgcc ctcgcccgcg ctcgccgcca ggagaccacg
      661 ctggcggtgg ccttcctcga cctcgacggc ttcaagcagg ccaacgacac ctacgggcac
      721 cagatcggcg acgccgtgct ctgcgaagtc gccacgcgcc tgcgccacca cgcccggtcc
      781 ggcgactcct acgcccgcct cggcggcgac gagttcatcg tgatcgccga gaacatcggc
      841 tcgccggcca acgccctcac cttcgcccgc cggatcggca cggcgatcgc cgagccgatc
      901 gccgccggtg agcacctcgt cgagatcggc gtcagcatcg gcctggcgct ggccccctcc
      961 ggccgagcat cgctggtcga cctgctcgcc tgcgccgacc acgccgccta ccgcgccaag
     1021 cgctcgaaga ccggcgccga gctctacgac gaggtcctcg atggcggcca gggcgccgtg
     1081 ctcgggttga tggacccggc accgggtccc accgagcccg tcgagatcac cgtcgactgg
     1141 gtcgacctcc gctcgtgatc gcccaccctc cttctccaga tgacgggcga gacgacgggt
     1201 cagccagcga gcagcccggc gtcgtcggtg acggtgacct cgccagtggc gaggtcgtac
     1261 atcatgccca ccacgaacgg ggcgtgaccc gcgggcgtgg cgagcgtgtc catcgaccgc
     1321 agcgcggtga ccgtggcgtc gaggttggcg ccggtggccc gaaccggatc ggcccccacc
     1381 acctggatca accggtcggc cacctcgccg gtgagctcgc ccagatggcc gtcggggtgg
     1441 tcgtcgctga gggcggcgag gaccgcgccg cacccgctgt ggccgagcac gaccacgagc
     1501 gacaggccca agacgcggac cccgaactcg atgctgccca gcgcgacgcc ggcgagcgac
     1561 tgtcccgccg tgcgcacggt caacaggtcg ccagcgccct ggtcgagcac gacctcgggc
     1621 ggaacccggg agtcgacgca gccgagcagg acggcgatgg ggtactggcc tgcgaccagg
     1681 gcctcgcggt ccgcctccgt gaggacgtgc gaggtcgggt tcccgtcgac gaagcgacgg
     1741 ttgccggtca ccagctcccg gagtgatgcg gccgccgccg ctcgtgcttg ctcgctggtg
     1801 gtggtgggca cggaaggcct tccggtcgag gggccggagc atcatgccgt ccccgatcgc
     1861 ctcggcgaaa gccgaaccgg cggtgaccac cggaggtgtc cggcgagagg tacgttcccg
     1921 ctggtcaggg acgcgcaccg tctggccctc gcggttcaag ggcaccccca tgacatgccg
     1981 atgatccctg gagaccccgg ttaggaacgg catgcgactc cacccgtcgg gcttcggcct
     2041 gcgaacccgg ttgctcgtct tggtcctggt tcccgcggtc ttgatggccg tcgtgggcgg
     2101 cgcggcgatc acgcggcagg cccacgccgc ggccaacctg gcggaggtcc acgacgagat
     2161 cggggtgctg gccgacctga cggagctgcg ccgcgccctg ctcggtgcgc gcgcaccgac
     2221 cgagctcgag gtccgagcgc aggccatcgg catcgatgag gacgccgcca tccgcctgct
     2281 cggcgtcgac cagctggagg gcgggagcct cgatgaggtg gccgagcgcc tccgagccct
     2341 gcccaccgag gcgcgcccgt tcacctcggc gcagctcggc gcgctccgcg ccgcgccgg
//
LOCUS       DATMFS010000581         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_65980, whole genome shotgun sequence.
ACCESSION   DATMFS010000581 DATMFS010000000
VERSION     DATMFS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65980"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..528)
                     /locus_tag="VNQ33_11055"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="VNQ33_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1018..1731
                     /locus_tag="VNQ33_11060"
     CDS             1018..1731
                     /locus_tag="VNQ33_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ98689.1"
                     /translation="MSTSDARRRGSNRSRRFSTDEVVDAALRCVERDGLDALTMRAVA
                     TELGASPMALYRYVDGREDLVARTVDRSLAGFDFPDLPAADAPGAELAAWLRAMAVEG
                     RIRLLRYPGTADHLLLHGPTGPWSILFMDQVCKVIALTGREPTEVAWAYDWLMTTAAA
                     YISKEARLGDGGSKAAVSLQQLAADVAKYPDLIPVVATFTGVVGDSYTRATNLVIDAL
                     TADPSPPGPASAGRSAPPA"
     gene            complement(1739..>2369)
                     /locus_tag="VNQ33_11065"
     CDS             complement(1739..>2369)
                     /locus_tag="VNQ33_11065"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWJ98690.1"
                     /translation="REVIDLVGGGPRSGAAIGVLPGVSGPVIPADHLDVALSYEGLGA
                     IGSSLGAGAFIVIDESDDPVAVAEGASRFLAVESCGQCQPCKQDGLAISAILGRLRAS
                     EPGARDEDELGSRLRTVADGARCFLATQHQQVVSSLIQAFPESFGAHLVGGAAPAARV
                     QIAEIDHVRDGEAVLDPGQATKQPDWSHGAVSSGAAPADRIDQRGGEGI"
BASE COUNT          345 a          877 c          818 g          329 t
ORIGIN      
        1 gtccacgacg gcgtcgttgc cggccttcac gccgggcagc ccctcgaaca ccggcgaatc
       61 caagaaggtc ttcgaggcgt agtagtagcc atcgagcagc acgatgacgt cgccgtcgtg
      121 gtcgccgagc acctcctcgg agatcatcac cgcgctgtcg taggccgggt cgaccttgcc
      181 ctcggcctgg gcggcgggcc gggagtagcc gatctcgccg acgagctccg agaggggcgc
      241 cttcggcgac atggcgaaca gcatccccat cgtgtcgccg aggatcgaca tcgtcggctc
      301 gtcccctccc acctcgccag cgacctcgcc gatgcggccc tcgatggcct cgacgatggc
      361 gtcgccctcg tcctccttcc cgaacgcagc cgcggtcgtg gtgacgacgt cgcgccacgg
      421 ctcgctgtag ggcagcagga ggaccggtgc caccttcgag aggtcgtcgt acgccgacag
      481 cagcgcccgc tccggggtga ggacgatgag atcgggcttg gcggcggcga cggcctcatg
      541 ttcacaccgc cttcgaccgt ggccggcgtg acgtcgacac cagcgtcctc cagcaggtca
      601 gcggcgatct cggagtggta cgaggcccag acctgcacgg gctcgatgcc gaaggtgagg
      661 gcgttcatgg cggcgtactc gtcgagggcg acgacgcgct gcggatcgac cgggacctcg
      721 acctctccca tctcggtctc caccgtccgc gtcgcgccgc caccgccacc cccaccgcca
      781 cgggtcgctt cggccgtcgg ctcggtgggc gaaggcgcgg acccggcatc gctgccaccg
      841 ctcccgcagg cggccgtgga cagcgcgagg acgacggcca gtgccgcgac gggacgacga
      901 agtgcgaagg tggatggcat cggacctacc tgtgacgtgg gctcgtgaga accggacagc
      961 ggatgcgtac gttgtacgtg tacgcagtaa gcatgaacgt aaaggatgct taacatcgtg
     1021 tcaacctccg acgctcgccg gcgcggatcg aaccgttcgc gccgcttctc caccgacgag
     1081 gtcgtcgacg ctgcgctgcg ctgcgtcgag cgcgacgggc tcgacgccct cacgatgcgg
     1141 gcggtggcca ccgagctcgg cgcctcgccc atggcgctct accgctacgt cgacggccgc
     1201 gaggacctcg tcgcccgcac cgtcgaccgg tcgctggctg gcttcgactt ccccgacctg
     1261 cccgcagccg acgctccggg ggcggagctc gcggcctggc tccgggcgat ggccgtcgag
     1321 gggaggatcc gcctgctccg ctacccgggc accgcggacc acctcctcct ccacgggcca
     1381 accgggccct ggagcatcct gttcatggac caggtctgca aggtgatcgc gctcaccggc
     1441 cgcgaaccca ccgaggtggc atgggcctac gactggctga tgacgaccgc ggcggcgtac
     1501 atctcgaagg aggcccgcct cggcgacgga gggtcgaagg ccgccgtgtc gctccagcag
     1561 ctcgccgccg acgtggccaa gtacccggac ctcatcccgg tcgtcgccac gttcaccggc
     1621 gtcgtggggg actcgtacac ccgagcgacc aacctggtga tcgacgccct cacggccgac
     1681 ccctccccgc ctggccccgc ctccgccggg cgctcggcgc cccccgcctg agggacggtc
     1741 agatgccctc gccgccgcgc tggtcgatcc ggtcggcggg cgccgcgccc gaactcacgg
     1801 cgccgtggga ccagtcgggc tgcttcgtcg cctgcccggg atcgaggacg gcctcgccgt
     1861 ctctgacgtg atcgatctcg gcgatctgca cccgggcggc cggcgccgcc ccacccacga
     1921 ggtgggcgcc gaacgactcg gggaaggcct ggatcaggct cgagaccacc tgctggtgct
     1981 gtgtggccag gaagcagcgg gcaccgtcgg ccacggtcct caggcgtgac ccgagctcgt
     2041 cttcgtcacg ggctccaggc tccgaggcgc gcaggcggcc gaggatggcg ctgatggcga
     2101 gcccatcctg cttgcacggc tggcactggc cgcacgactc cacggcgagg aaccgcgatg
     2161 cgccctcggc gacggccacg ggatcgtcgg actcgtcgat gacgatgaac gcaccggcgc
     2221 cgagcgagga cccgatggcg cccaggccct cgtaggagag cgcgacgtcg aggtgatcgg
     2281 ccgggatcac gggaccggac acgcccggca gcacgccgat ggccgcaccg ctccgggggc
     2341 cgccgcccac gaggtcgatc acctcgcgc
//
LOCUS       DATMFS010000582         4227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_159877, whole genome shotgun sequence.
ACCESSION   DATMFS010000582 DATMFS010000000
VERSION     DATMFS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159877"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..954
                     /locus_tag="VNQ33_11070"
     CDS             154..954
                     /locus_tag="VNQ33_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98691.1"
                     /translation="MASSSSAKKVARVAAKSGSGKPVGASAGTGRNWLFGLGIVVIIA
                     LGAGVVALARNENGGYGANSTPPKANLQNGDPFDHWHAAFAVEVCGTELAPLQDGPQD
                     VQGIHTHGDGLVHIHPFTRAAAGKRATMQRFFNQVSLKVTDTGFELPDGMTMKDGSTT
                     VKEGETTCAGKPGELVMAHWKDAATAAGTKPDKIYRKDFGSIRFTEDLGAFTLAFVPK
                     GSTDIEAPSSAAEIGTLGACDGANPPPSCETDGSTATVPDTSGSESGG"
     gene            963..1973
                     /locus_tag="VNQ33_11075"
     CDS             963..1973
                     /locus_tag="VNQ33_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWJ98692.1"
                     /translation="MLVGGFGTRLRPLTLTRPKQMLPILHRPMIEHVLEHLADHGITE
                     AVLSMGYKPDAFADAYPDGQHAGVTLHYAIEPEPLDTAGAIRFAALDAGIGERFVVVN
                     GDVLTDLDIGALVAFHDEHGGEGTIALHQVEDPSAFGVVPTDPDGRVQAFVEKPSREE
                     APTDLINAGTYVLEASVLDRIADGRKVSIEREVFPAMVADRVLYAMSGNTYWIDTGTP
                     SKYLQSQLDLLDGLRGEPASGVHPGATVADDAKVVRCVVGDGAVIESGAVVHDAVLLA
                     GARVGEGAVVERAVIGQDAQVGAGARIDDLAVLGDGAVVGPRLRVSGGTVGVDEHLET
                     GS"
     gene            1994..2950
                     /locus_tag="VNQ33_11080"
     CDS             1994..2950
                     /locus_tag="VNQ33_11080"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWJ98693.1"
                     /translation="MRALVTGGAGFIGSTLVDRLLAEGHEVDVVDDLSSGSMANLAEA
                     RGNGDHKFQFHRMDIRDASLIELIERRQPEVIFHLAAQIDVRVSVSDPVHDAMINVIG
                     SLHVLEGARRGGTRKVVFASSGGTIYGVVADEDLPADESLPQRPVSPYGITKKVVGDY
                     LYAYRELHQLEFTALALANVYGPRQDPHGEAGVVAIFAGRLLRDEPCTIFGDGAQTRD
                     YVFVDDVVDAFVRAADKGDGLIINIGTATETTVNDLYATMARVAGVDRVATLAAARPG
                     ELARSALDASRAEMQLGWKPWTDLATGSAAVLEFFRTRDTSA"
     gene            complement(3032..3745)
                     /gene="cofE"
                     /locus_tag="VNQ33_11085"
     CDS             complement(3032..3745)
                     /gene="cofE"
                     /locus_tag="VNQ33_11085"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWJ98694.1"
                     /translation="MSELRVFGVTGIGEIRPRDDLAGIIASATADHHGGLVDGDVVVV
                     TQKIVSKAEGQLVAVDPDDPLSHKALVEEETVRVLRRRGDLVISETKHGFVCANAGID
                     LSNVELGYAALLPEDSDRSARRIRDGIRARAGVEVGVIVSDTFGRTWRRGLTDVAIGC
                     AGIGAIVDHRGTHDTQGRELQVTEVAVVDELAGAADLVLGKATGLPVAVIRGVDPSWL
                     RTSSVGDEIVRDPAEDLFR"
     gene            complement(3742..>4227)
                     /locus_tag="VNQ33_11090"
     CDS             complement(3742..>4227)
                     /locus_tag="VNQ33_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HWJ98695.1"
                     /translation="EIGFQEYFVQRRHGVPVSSVRFDGADAAKPGPDVLDALAGADRV
                     VLAPSNPIVSIDPVLAVPGVREALVARRADTVAVSPIVAGSALKGPADRLLVELGHEA
                     TVVGVARLYAPLAEALVIDHADADLAGAVAAEGIRPILAESVMRTPDVAADLARTVLA
                     G"
BASE COUNT          618 a         1518 c         1510 g          581 t
ORIGIN      
        1 agcggcccag atgggtgcgg cggcccggcg gttcgtggag cggtgggcgt cgcctgcggg
       61 cgttgccgcc gcgtacgaag acctgttcaa cgagctgatc gggcgctgac ccgaatcctt
      121 ccgggagggg ccggcccact agcgtggcgc cccatggcat cgtcttcatc ggccaagaag
      181 gtcgcgcgcg tcgcagccaa gagcggctcc ggaaagccgg ttggcgcatc ggcaggaacc
      241 ggccgcaact ggctgttcgg cctcggcatc gtggtcatca tcgccctggg cgccggcgtg
      301 gtcgccttgg cccgcaacga gaacggcggg tacggcgcca actccacgcc gcccaaggcg
      361 aacctgcaga acggcgatcc gttcgaccac tggcacgccg ccttcgccgt cgaggtgtgc
      421 ggtaccgagc tggcaccgct ccaggacggc ccgcaggatg tccagggcat ccacacgcac
      481 ggtgacggcc tcgtccacat ccaccccttc acgcgggccg cggcaggcaa gcgcgccacc
      541 atgcagcgct tcttcaacca ggtcagcctg aaggtcaccg acaccggctt cgagctgcct
      601 gatggcatga ccatgaagga cggctccacc accgtgaagg aaggcgagac cacgtgtgcc
      661 ggcaagcccg gcgagctggt catggcccac tggaaggacg cggccacggc ggcgggcacc
      721 aagcccgaca agatctaccg caaggacttc ggcagcatcc gtttcaccga ggacctgggt
      781 gccttcacct tggcgttcgt gcccaaggga tcgacggaca tcgaggcgcc atcgagcgct
      841 gccgagatcg gcaccctggg tgcctgtgac ggagcgaacc ctccgccgag ctgcgagacc
      901 gatggatcga ccgccacggt gcccgacacc agcggctccg agtcgggcgg atgagggcgg
      961 tcgtgctggt cgggggcttc ggcacccggc tccggccgct caccctcacc cggccgaagc
     1021 agatgctgcc gatcctgcac cggccgatga tcgagcacgt cctcgagcac ctggccgacc
     1081 acggcatcac cgaggccgtg ctgtcgatgg gctacaagcc cgacgcgttc gccgacgcct
     1141 accccgacgg ccagcacgcc ggcgtcaccc tgcactacgc catcgagccc gagccgctcg
     1201 acacggcggg cgccatccgc ttcgcggcac tcgatgccgg tatcggcgag cgcttcgtcg
     1261 tggtcaacgg cgacgtgctg accgatctcg acatcggtgc gctcgtggcc ttccacgacg
     1321 agcacggcgg cgagggcacc atcgccctcc accaggtcga ggacccctcg gcgttcggcg
     1381 tggtccccac cgaccccgac ggccgagtcc aggccttcgt cgagaagccc tcccgcgagg
     1441 aggcgcccac ggacctgatc aacgccggca cgtacgtgct ggaggcatcc gtgctggacc
     1501 gcatcgccga tggccgcaag gtgtccatcg agcgcgaggt cttcccggcg atggtggccg
     1561 accgggtgct ctacgccatg tccggcaaca cctactggat cgacaccggg accccgtcga
     1621 agtacctgca gagccagctc gacctgctcg acggtctccg cggcgaaccc gcctcgggcg
     1681 tgcaccccgg tgcgacggtg gccgacgatg ccaaggtcgt ccgctgcgtg gtcggtgacg
     1741 gggcggtgat cgagtccggt gccgtcgtcc acgatgcggt gttgctggcc ggtgcccgcg
     1801 tcggtgaggg tgccgtcgtc gagcgcgccg tcatcggaca ggacgcgcag gtcggtgccg
     1861 gtgcccgcat cgacgacctc gccgtgctgg gtgacggggc tgtcgtcggc ccgcgcctgc
     1921 gggtgtcggg agggaccgtc ggcgtcgacg agcacctcga gaccggatcc tgatccaagg
     1981 ggagcaccaa ccaatgcgcg cactcgtgac cggcggggcc ggcttcatcg gctcgaccct
     2041 cgtcgaccgc ctgctggccg agggccacga ggtcgacgtc gtcgacgacc tctccagcgg
     2101 ttcgatggcg aacctcgccg aggcccgggg caacggcgat cacaagttcc agttccaccg
     2161 gatggacatc cgcgacgcct cgctgatcga gctcatcgag cgccgccagc ccgaggtgat
     2221 cttccacctg gccgcccaga tcgacgtgcg ggtgtcggtc tcggacccgg tccacgacgc
     2281 catgatcaac gtgatcggct cgctccacgt gctcgagggc gcccgacgcg gggggacccg
     2341 caaggtcgtg ttcgcctcca gcggcggcac gatctacggc gtggtcgccg acgaggacct
     2401 cccggccgac gagtcgctcc cgcagcgccc ggtgtcgccc tacggcatca ccaagaaggt
     2461 ggtgggcgac tacctctacg cctaccgcga gctgcaccag ctcgagttca cggccttggc
     2521 gctggccaac gtctacggcc cccgccaaga cccccacggc gaggccggcg tcgtcgccat
     2581 cttcgccggg cgcctgctcc gtgacgaacc gtgcacgatc ttcggcgatg gtgcccagac
     2641 ccgcgactac gtgttcgtcg atgacgtggt cgacgccttc gtgcgcgccg ccgacaaggg
     2701 cgacgggctc atcatcaaca tcggcacggc caccgagacg acggtcaacg acctctacgc
     2761 cacgatggcc cgggtcgccg gcgtcgaccg ggtggcgacg ctcgccgccg cccgtcccgg
     2821 tgagctggcc cgctcggcgc tcgacgcgtc gcgcgccgag atgcagctgg gctggaagcc
     2881 ctggaccgac ctcgccaccg gcagcgccgc ggtcctcgag ttcttccgca cccgcgacac
     2941 ctcggcctga cccggcccgg ccgcgggtga gcgcacgcag cgccgcggcg cggtggccgt
     3001 gccctcgcct tgggtccggc gggaccggag gtcagcggaa gaggtcttcg gccgggtcgc
     3061 ggacgatctc gtcgccgacg ctgctggtgc gcagccagga ggggtcgacc ccccggatca
     3121 ccgccacggg gaggcccgtc gccttgccga gcaccaggtc ggcggcaccg gcgagctcgt
     3181 cgacgacggc gacctcggtc acctggagct cgcggccctg ggtgtcgtgg gtgccgcggt
     3241 ggtcgacgat ggcgccgatg ccggcgcacc cgatggcgac gtcggtgagg ccgcggcgcc
     3301 aggtgcgtcc gaacgtgtcg ctcacgatga cgcccacctc gacgccggcc cgggcccgga
     3361 tgccgtcgcg gatgcggcgg gcggaccggt cgctgtcttc gggcaggagc gcggcgtagc
     3421 cgagctccac gttggacagg tcgatgccgg cgttggcgca cacgaagccg tgcttggtct
     3481 cggagatcac gaggtctccc cggcggcgga gcacccggac ggtctcctcc tcgacgaggg
     3541 ccttgtggct cagcgggtcg tcggggtcga cggcgacgag ctggccctcg gccttcgaca
     3601 cgatcttctg ggtgacgacg acgacgtcgc catcgaccag cccgccgtgg tggtcggcgg
     3661 tggccgaggc gatgatcccg gcgaggtcgt cgcgcgggcg gatctcgccg atgccggtga
     3721 cgccgaagac ccgcagctcg ctcatccggc caggaccgtc cgggccaggt cggcggcgac
     3781 gtccggtgtc cgcatgacgc tctcggccag gatcgggcgg atgccctcgg cggccaccgc
     3841 gccggccagg tcggcatcgg cgtggtcgat cacgagggct tcggccaacg gggcgtagag
     3901 gcgggcgacc ccgacgacgg tggcctcgtg gcccagctcg acgagcagcc ggtcggccgg
     3961 gcccttgagg gcggagccgg cgacgatggg ggagacggcg acggtgtcgg ctcggcgggc
     4021 gacgagcgcc tcccggacgc cggggacggc gaggacgggg tcgatcgaca cgatcgggtt
     4081 cgacggggcg aggaccaccc gatcggcgcc ggccagggca tcgagcacgt caggaccggg
     4141 cttcgcagcg tcggcgccgt cgaagcggac ggacgatacc gggacgccgt gccggcgctg
     4201 gacgaagtac tcctggaacc cgatctc
//
LOCUS       DATMFS010000583         7128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_228728, whole genome shotgun sequence.
ACCESSION   DATMFS010000583 DATMFS010000000
VERSION     DATMFS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228728"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VNQ33_11095"
     CDS             <1..512
                     /locus_tag="VNQ33_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWJ98696.1"
                     /translation="KRIVRSGPNTLLPYQKKPRDRTMGDDDIAFADFGPIFDGWEADY
                     GRTWVIGDDPDKIRLRDDLAEVFTAGRQFFEDRPDITGQDLYTEVTRLAADRGWGFGN
                     HHCGHLVGEFPHEDFTGGRPASHLMVGSTEPMRRLDPSGRIAHWILEIHLIDEDRRFG
                     GFYEQLLTL"
     gene            complement(565..1269)
                     /locus_tag="VNQ33_11100"
     CDS             complement(565..1269)
                     /locus_tag="VNQ33_11100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWJ98697.1"
                     /translation="MPRAVPQPVLIVQHLEPEGPALIATALAERGRAVDVVRVDEGAP
                     LPADLAGHGGLVVMGGPMSAASDDGFPSRMAEVALISDALQRGRPMLGVCLGAQLLAV
                     AAGASIRSGGEPEIGWGTVQVSTGAGGDALFHGLGRELWVLHWHGETFDLPAGAVHLA
                     SSAAYPNQAFRMGASAWGVQFHPEVDEAAVSRFVASFGHEAADPAAITRDAPSRLSAS
                     AAERMRIFDRFAGLIV"
     gene            complement(1327..1731)
                     /locus_tag="VNQ33_11105"
     CDS             complement(1327..1731)
                     /locus_tag="VNQ33_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98698.1"
                     /translation="MTEPRQATPTPAERRPLDERPVHTTDLPDTPTRDRSIPATAWIE
                     APPELLALADDLPAPSTGATARYLRRIGPWLLWRSGPARGADARYWVGRADDLAVQFT
                     FDLVPDGTGSGHGPTASHTRFRTWKEDLRDHA"
     gene            complement(1758..2366)
                     /locus_tag="VNQ33_11110"
     CDS             complement(1758..2366)
                     /locus_tag="VNQ33_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ98699.1"
                     /translation="MDTLIWSDAEAEIHKVVVGPMDNNVYVLRCGQTGDAVLLDAADE
                     HEQLLALSQQLGVREVLETHGHHDHIQAVPQMRDAGYSVHITAEDAAMLPSYDQILED
                     ASIIPVGRLRLETVFTPGHTPGSMCFKVAGKPVLFSGDTLFPGGPGTTKFGGHFPTIL
                     RSLEDRLFSPLAADTIVLPGHGDSTTIGAESPHLQEWADRGW"
     gene            complement(2393..3019)
                     /locus_tag="VNQ33_11115"
     CDS             complement(2393..3019)
                     /locus_tag="VNQ33_11115"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWJ98700.1"
                     /translation="MITEDRPGLRERKKQRTHDDLQRVALQLFTERGYHDVTIEEIAA
                     EVDVSPRTFYRYFSSKEDVVLGSVPEMVAATSAALAARPRTETVMGSIRAVTLDLVSE
                     FASDVEANRIRTGIIAASPELQQRSAERQPIMEAVLVPFVAERLGMDPEQDLAPQVIV
                     SCTVAVTRAAIERWTAHGSDRGLAETIDEALALVDAGLHSITPADPRP"
     gene            3155..5377
                     /locus_tag="VNQ33_11120"
     CDS             3155..5377
                     /locus_tag="VNQ33_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWJ98701.1"
                     /translation="MSKYLYRLGHGCVRRRRTVLVAWIVVLFGVGVLASTVGGPLSDQ
                     FSIPGTESQQAMDLLDQRFPQASGSSARVVFAVDEGRLDDAGAKAGIEATLAQLGKAD
                     GVRAVSDPFEAGTVSPDGSIGFADVQYGSDASEVTEAQLDALYGASGPAEAAGVQVEH
                     GGEVKRQEEPGHSSELIGLGVAVIVLLLSFGSLVAMGIPIVTALIGVGIGLSGITVVA
                     GLTDVSSSAPTLATMIGLAVGIDYALFIVTRHRQNMAEGHDVAEAAARATATAGGAVV
                     FAGMTVVIALLGVLVIGIPFLTAMGLGAALTVFLAVLIAVSLLPALLGFAGTNIDRWR
                     IGRSRTGSAEEAEASLSGRFARRVIARPGVSLAVGLGIIAVLAVPFLSMRLGMTDAGA
                     SPKDTTERKAYDLLAEGFGPGFNGPLTLVVDTREAADPEAALTAVSAAVGDDPGVAVV
                     GPPMANEAGDTSIVSVIPATSPSSAETEELVHHLRHDVVAPVAERTGADVAIAGQTSV
                     LIDVADKMADALPLFMGLVIGLTMILLLVVFRSLLVPVKAAIAILLSIGASFGVLVAV
                     FQWGWMKDLIGLQETVPIISFLPLLMFAILFGLSMDYEVFILSRIREDYVRTGDARRS
                     VLTGLTSSARVITAAALIMISVFASFVLGEDPIIKMFGVGFATAVLLDATVVRMIVVP
                     ATMALFGDRAWWLPGWLDRIIPDLDVEGEHLIEALDHASDGSGGGDRVDDADRVPVPA
                     "
     gene            complement(5403..5807)
                     /locus_tag="VNQ33_11125"
     CDS             complement(5403..5807)
                     /locus_tag="VNQ33_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ98702.1"
                     /translation="MTLALDTSALLGRYLSGPHRALVLEAMAADPVWCASALAQAEAL
                     GMVDRVCDLATDGDRVRRALRDDWERIHVVPVDQRCLDRAAELTREQPLRTVDALHLA
                     AADRLPRPTTFLTFDPRQIPVALAMGFDVVSS"
     gene            complement(5804..6088)
                     /locus_tag="VNQ33_11130"
     CDS             complement(5804..6088)
                     /locus_tag="VNQ33_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWJ98703.1"
                     /translation="MDERLGIRDLRGRLTSLVRRAGEGERIVVTIAGRPVAQLGPVEP
                     TGTQVTIDDLAARGLLVPARRGDRPADPDTRIDTWTGGRLDRALREVRGA"
     gene            6201..>7128
                     /locus_tag="VNQ33_11135"
     CDS             6201..>7128
                     /locus_tag="VNQ33_11135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98704.1"
                     /translation="MNFAFNEEQEELRKTVRAFLEAKSSEAAVREQMDTEAGYDAAVW
                     QQMGEQLGLQGLAIPEEFGGSGYGYIELVVILEEMGRSLLCAPFFSSVVLAANTLIHS
                     GDDQAKADLLPGIATGETIATLAFTEENGRWDEAGITATATPDGDTWKLGGTKMYVLD
                     GHTASLLLVAARTDAGVSLFAVDGEGAGVTRTALSTMDQTRKQAKVELDSAAGRLIGA
                     DGEGWAVLERVLDLAAVALAAEQVGGAQACLDMAVQYAKDRVQFGRPIGSFQAIKHKC
                     ADMLLEVESAKSAAYYAGWAASELNDELPSVAS"
BASE COUNT          942 a         2605 c         2548 g         1033 t
ORIGIN      
        1 acaagcgcat cgtccgctcc gggcccaaca cgctcctccc gtaccagaag aagccgcgcg
       61 accggaccat gggcgacgac gacatcgcgt tcgccgactt cggcccgatc ttcgacggct
      121 gggaagccga ctacggccgc acctgggtca tcggcgacga cccggacaag atccggctcc
      181 gagacgacct cgccgaggtc ttcaccgccg gccggcagtt cttcgaggac cgccccgaca
      241 tcaccggcca ggacctctac accgaggtca cccggctggc cgctgatcgc gggtggggct
      301 tcggcaacca ccactgcggc cacctcgtgg gcgagttccc ccacgaggac ttcactggtg
      361 gccgccccgc ctcgcacctc atggtcggca gcaccgagcc catgcgcagg ctcgacccct
      421 ccgggcggat cgcccactgg atcctcgaga tccacctcat cgacgaggac cgccgcttcg
      481 gcgggttcta cgagcagctc ctcaccctct gacgccgcca cggaacccgg tcgcgccgta
      541 cccgcccacc ggaccgggcc gccgtcacac gatcaggccg gcgaaccggt cgaagatgcg
      601 catccgctcc gccgcggagg cgctgagccg cgagggcgcg tcgcgggtga tggctgctgg
      661 gtcggcggcc tcgtggccga acgaagccac gaaccgcgac accgccgcct cgtcgacctc
      721 ggggtggaac tgcacgcccc acgcggatgc acccatccgg aacgcctggt tcgggtacgc
      781 agctgacgat gccaggtgga ccgccccggc gggcaggtcg aaggtctcgc cgtgccagtg
      841 cagcacccac agctcgcgtc cgagcccgtg gaacagcgcg tcgcctcccg ccccggtgga
      901 gacctgcacc gtcccccacc cgatctccgg ctccccgccc gaccggatcg aagcaccggc
      961 cgcgacagcg aggagctggg cgcccaagca cacaccgagc atcggtcgac cacgctgcag
     1021 cgcatcggag atgagcgcca cctccgccat ccggctcggg aagccgtcgt cgctcgcggc
     1081 agacatcggg ccgcccatca ccaccagtcc gccatggccg gccaggtcgg ccggtagcgg
     1141 agcaccctcg tccacccgca ccacgtcgac cgcgcgtcct cgttcggcga gggccgtggc
     1201 gatcaaggcc ggcccctccg gctcgagatg ctgcacgatg agcacgggtt gcggcacggc
     1261 acggggcacc gtgccgttct acccgctcgt cgacggccgc tcaccccgtg ccgccagcac
     1321 cgtcgctcac gcgtggtcgc gcaggtcttc cttccaggtg cggaagcggg tgtgcgaggc
     1381 ggtcgggccg tggccggatc cggtgccgtc ggggacgagg tcgaaggtga actgcacggc
     1441 gaggtcgtcg gcgcggccga cccagtagcg ggcatcggcg cctcgcgctg gtcccgagcg
     1501 ccacagcagc cacgggccga tgcgccggag gtaccgggcg gtcgcaccgg tcgacggcgc
     1561 cggcaggtcg tcggccagcg ccagcagctc gggcggtgcc tcgatccagg cggtcgccgg
     1621 gatcgaccgg tcgcgcgtgg gcgtgtcggg caggtcggtc gtgtgcaccg gccgctcgtc
     1681 gagcggccgc cgctcggccg gggtgggggt tgcctggcgc ggttcggtca cggggcgatc
     1741 ctcgcacgcg aggtcggcta ccacccgcgg tcggcccact cctgcaggtg gggggactcc
     1801 gctccgatgg tcgtcgagtc gccgtgaccg gggagcacga tggtgtcggc ggccagcggc
     1861 gagaacaggc ggtcctcgag cgagcggagg atggtgggga agtggccgcc gaacttcgtg
     1921 gtgcccggtc cgccggggaa gagggtgtcg ccgctgaaga ggacgggctt gcccgcgacc
     1981 ttgaagcaca tcgaccccgg ggtgtggccc ggggtgaaga ccgtctccag ccggaggcgg
     2041 ccgaccggga tgatcgatgc gtcctcgagg atctggtcgt agctcgggag catcgccgcg
     2101 tcctcggcgg tgatgtgcac ggagtacccg gcgtcgcgca tctggggcac ggcctggatg
     2161 tggtcgtggt ggccgtgcgt ctcgagcacc tcgcgcaccc ccagctgctg gctcagcgcc
     2221 agcagctgct cgtgctcgtc ggcggcgtcg agcaggacgg cgtcgccggt ctggccgcac
     2281 cgcagcacgt agacgttgtt gtccatgggg cccacgacga ccttgtggat ctcggcttcg
     2341 gcgtccgacc agatcagcgt gtccatgccg ccagtctgcc gtggcggcgc cgctacgggc
     2401 gagggtcggc gggggtgatc gagtgcagcc cggcgtcgac gagcgccagg gcctcgtcga
     2461 tggtctcggc gaggccgcgg tcggagccgt gcgcggtcca gcgttcgatg gccgcccggg
     2521 tcacggcgac cgtgcacgac acgatcacct gcggcgccag gtcctgctcg gggtccatcc
     2581 ccagccgctc ggccacgaag ggcacgagca cggcctccat gatcggctgg cgctcggcgc
     2641 tgcgctgctg gagctccggg ctggcggcga tgatcccggt gcggatccgg ttggcctcga
     2701 cgtcgctcgc gaactcggac acgaggtcga gggtgacggc gcggatggat cccatgaccg
     2761 tctcggtgcg gggacgggcg gcgagcgcgg cgctggtggc cgccaccatc tccgggacgc
     2821 tgccgaggac cacgtcctcc ttggaggaga agtagcggta gaaggtgcgg ggcgagacgt
     2881 cgacctcggc ggcgatctcc tcgatggtga cgtcgtggta gccgcgctcg gtgaacagct
     2941 ggagcgcgac ccgctgcagg tcgtcgtgcg tgcgctgctt cttgcgctcc cggagccccg
     3001 gccggtcctc ggtgatcacg ccgccagcct acctgggggt ctgacgttcg tcatgcatgt
     3061 gccggatgtc atctatgcat attggcaggt ctgccaatat gctcctcctg ctcgacgagc
     3121 gggcccgccg cgtcgtctgt cccgagagga cctcatgtcg aagtacctgt accgcctggg
     3181 ccacgggtgc gtgcgccgcc gcaggaccgt gctcgtcgcc tggatcgtgg tgctgttcgg
     3241 cgtcggtgtc ttggcgagca ccgtcggcgg gccgctgagc gaccagttct cgatccccgg
     3301 caccgagtcc cagcaggcca tggacctgct cgatcagcgc ttcccgcagg cctcgggctc
     3361 gtcggcccgc gtggtgttcg ccgtcgacga gggccggctc gacgacgccg gcgccaaggc
     3421 cggcatcgag gcgaccctgg cccagctcgg gaaggccgac ggggtgaggg cggtctccga
     3481 cccgttcgag gccggcaccg tctcccccga cgggagcatc gggttcgccg acgtgcagta
     3541 cggctccgat gcgagcgagg tcaccgaggc ccagctcgac gccctctacg gcgcctcggg
     3601 tccggctgag gccgcgggtg tccaggtcga gcacggcggc gaggtcaagc gccaggagga
     3661 gccggggcac agctccgagc tcatcggcct cggcgtggcc gtgatcgtgc tgctgctgtc
     3721 gttcggctcg ctggtcgcca tgggcatccc gatcgtgacc gcgctcatcg gcgtgggcat
     3781 cggcctctcg ggcatcaccg tcgtggccgg cctcaccgac gtgtcgtcgt cggctcccac
     3841 cctcgccacg atgatcggcc tcgcggtcgg catcgactac gcgctcttca tcgtcacccg
     3901 gcaccgccag aacatggccg agggccacga cgtcgccgag gcggcggcgc gggccaccgc
     3961 caccgctggc ggcgccgtcg tgttcgccgg gatgaccgtg gtcatcgccc ttctcggcgt
     4021 gctcgtcatc ggcatcccgt tcctcacggc gatgggcctc ggcgccgccc tcaccgtgtt
     4081 cctcgccgtg ctgatcgccg tgtccctgct cccggccctg ctcggcttcg ccggcaccaa
     4141 catcgaccgc tggcgcatcg gccggtcccg caccggttcg gccgaggagg ccgaggccag
     4201 cctcagcggc cgcttcgccc gcagggtcat cgcccgcccc ggcgtgtcac tggccgtcgg
     4261 gctcggcatc atcgcggtgc tcgccgtgcc gttcctctcg atgcgcctgg gcatgaccga
     4321 cgccggcgcc tcgccgaagg acaccaccga gcggaaggcc tacgacctgc tggccgaggg
     4381 cttcgggccc ggcttcaacg gtccgctcac cctggtggtc gatacccggg aggcggccga
     4441 tcccgaggcg gccctgaccg ccgtctccgc cgcggtgggc gacgaccccg gcgtcgccgt
     4501 cgtcggtccg ccgatggcga acgaggccgg ggacacctcg atcgtctcgg tcatcccggc
     4561 cacctcgccg tcgtcggccg agaccgagga gctggtccac cacctccgcc acgacgtggt
     4621 ggcccccgtc gccgagcgca ccggcgccga cgtcgccatc gccggccaga cctccgtcct
     4681 gatcgacgtg gccgacaaga tggccgacgc cctgccgctg ttcatggggc tcgtcatcgg
     4741 cctgaccatg atcctgctgc tcgtcgtgtt ccggtccctg ctggtgccgg tcaaggcggc
     4801 catcgccatc ctgctcagca tcggggcgag cttcggcgtg ctggtcgccg tcttccagtg
     4861 gggctggatg aaggacctga tcggcctcca ggagacggtg ccgatcatca gcttcctgcc
     4921 cctgttgatg ttcgccatcc tcttcggcct gtcgatggac tacgaggtct tcatcctctc
     4981 ccgcatccgt gaggactacg tgcggaccgg cgatgcccgc cgcagcgtgc tgacgggtct
     5041 caccagctcg gcccgggtca tcaccgccgc ggcgctcatc atgatcagcg tgttcgccag
     5101 cttcgtgctg ggcgaggacc cgatcatcaa gatgttcggc gtcgggttcg cgaccgcggt
     5161 gctgctcgac gcgaccgtcg tccggatgat cgtggtgccc gccaccatgg cgctgttcgg
     5221 cgatcgagcg tggtggctcc cgggctggct cgaccgcatc atcccggacc tcgacgtcga
     5281 gggcgagcac ctcatcgagg ccctcgacca cgcgtccgac gggtccggcg gcggcgatcg
     5341 ggtcgacgac gccgaccggg ttcctgtccc cgcctgagct cggagcggtc agggcgcccg
     5401 tctcaggacg agacgacgtc gaagcccatc gccagggcga cggggatctg gcgcgggtcg
     5461 aaggtgagga aggtggtggg ccggggcagc cggtccgccg ccgccaggtg cagggcgtcg
     5521 acggtgcgca gcggctgctc gcgggtcagc tccgcggcgc ggtcgaggca gcgctggtcg
     5581 acgggcacga cgtggatccg ctcccagtcg tcgcgcaggg cgcggcggac ccggtcgccg
     5641 tcggtcgcga ggtcgcacac ccggtccacc atgccgaggg cctcggcctg ggccagggcc
     5701 gacgcgcacc acaccgggtc ggctgccatg gcctcgagca cgagggcgcg gtgcggcccg
     5761 gagaggtagc ggccgaggag ggccgaggtg tcgagggcca gggtcatgcg ccgcgcacct
     5821 cgcgcagggc ccggtccagg cggccgccgg tccacgtgtc gatccgggtg tcggggtcgg
     5881 cgggccggtc gccgcgccgg gcgggcacca gcaggccccg cgccgccagg tcgtcgatcg
     5941 tgacctgcgt gcccgtcggc tccaccgggc cgagctgggc cacgggccgc ccggcgatgg
     6001 tgacgacgat gcgctcgccc tcgccggctc gccgcacgag cgacgtcagc cggccccgca
     6061 ggtcccggat gccgaggcgc tcgtccatcg cccaagtgta cgcacgtgta cgcgcccgga
     6121 cgggcgagga gcggcggcgg tggggcaggg ttcggggacc ggtcacctcc cgaccggccc
     6181 gcaccaccaa ggggaccagc gtgaacttcg cgttcaacga ggagcaagaa gagctccgca
     6241 agaccgtccg ggccttcctc gaggccaagt cgtccgaggc cgccgtgcgc gagcagatgg
     6301 acaccgaagc cggctacgac gctgcggtct ggcagcagat gggcgagcag ctcggcctcc
     6361 agggcctggc gatccccgag gagttcggcg gctcgggcta cggctacatc gagctcgtcg
     6421 tgatcctcga ggagatgggc cgctcgctgc tgtgcgcccc gttcttctcg tcggtcgtgc
     6481 tggctgccaa cacgctgatc cactccggcg acgaccaggc caaggccgac ctgctgcccg
     6541 gcatcgcgac cggcgagacc atcgccacgc tggccttcac cgaggagaac ggccgctggg
     6601 acgaggcggg catcactgcc accgccaccc ccgacggcga cacctggaag ctcggtggca
     6661 cgaagatgta cgtgctcgac ggccacaccg cctcgctgct gctggtcgcc gcccgcaccg
     6721 atgccggcgt gagcctcttc gccgtcgacg gcgagggcgc cggggtcacc cgcaccgccc
     6781 tgtccaccat ggaccagacc cgcaagcagg ccaaggtcga gctggacagc gccgccggcc
     6841 gcctcatcgg cgccgacggc gagggctggg ccgtcctcga gcgggtgctc gacctggccg
     6901 cggtggccct ggctgccgag caggtcggcg gggcccaggc gtgcctcgac atggccgtcc
     6961 agtacgccaa ggaccgcgtc cagttcggcc gccccatcgg ctcgttccag gccatcaagc
     7021 acaagtgcgc cgacatgctg ctcgaggtcg agtcggccaa gtccgccgcc tactacgccg
     7081 gctgggccgc ctcggagctc aacgacgagc tgccctcggt cgcctcgc
//
LOCUS       DATMFS010000584         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_114818, whole genome shotgun sequence.
ACCESSION   DATMFS010000584 DATMFS010000000
VERSION     DATMFS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114818"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1593)
                     /locus_tag="VNQ33_11140"
     CDS             complement(79..1593)
                     /locus_tag="VNQ33_11140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWJ98705.1"
                     /translation="MSGRSTNGIIAAGHVRAADAAASILERGGNAFDAIIAAGFASSV
                     CEPGFTSLAGGGFLLARSPELGDVLFDFFVDTPGRGLGAGAVAPAFDEVSVDFGSATQ
                     TFHCGPGSVAVPGVLAGYLHVHERLGRLPLADLVAPAVALAAGGVEVTATQSSDFTLL
                     APILARTEASRAVFFPDGRLLQAGDTLRNPDLAAFLDALGRGDGETFYAGELAARLVD
                     QLSVDGGLITAADLASYRVIERVPLRARYRDRTLLTNPPPTFGGALLALALRRIEAAG
                     PPLPAGSPERAVQLARIMAEVDRDRADGHAEVIAALQGDLGDDPGSRPLMTRGTTHVT
                     VADAEGNVAAMTTSNGECSGDVISGTGICCNNMLGEDDLHPDGFHAAPPGVRVASMMS
                     PTFVLSPGGDVELALGSGGSKRIRSALLQVLVATIDDGEPLDDAVLAPRIHWDEDHVE
                     AEPGLDPAVLTALEAGHAVNVWPAPSMYFGGVHAVAPGRTGAGDPRRGGSVRTV"
     gene            complement(1690..>2404)
                     /locus_tag="VNQ33_11145"
     CDS             complement(1690..>2404)
                     /locus_tag="VNQ33_11145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ98706.1"
                     /translation="DPLGCLRSTKNYPSIARTKIKAAKFKDVSCSGATTTHFFNEQGV
                     TPGPNQPQFNSLNANTKVVTIGIGGNDIGFSGIVKNCATLDPFSAGCKGDYVTGGHDL
                     LAEKIAATAPKIDQVLVEAKKRAPGAKIFVVGYPTIIPETGSGCWPTVPIVAGDIAYL
                     RGVAKGLNTMLKTRAQANGVHYVDTATSSIGHDMCSSSKWVEAIVPTTDAAPVHPNAK
                     GMANTGNVVAAAINAVVTA"
BASE COUNT          303 a          796 c          957 g          348 t
ORIGIN      
        1 gcccggagcg tcggtcacga ccgagcgcgc gtgcaccggt ggcgtcgccc acgtgtgctg
       61 cacgcgccgc gtcggtggtc agacggtgcg gaccgagccg ccgcggcggg gatcgccggc
      121 gccggtgcgg ccgggggcga cggcgtggac gccgccgaag tacatgctcg gggcgggcca
      181 gacgttgacg gcgtgaccgg cttccagcgc ggtgagcacg gccgggtcga ggccgggctc
      241 ggcctcgacg tggtcctcgt cccagtggat gcggggtgcg agcacggcgt cgtcgagcgg
      301 ctcgccgtcg tcgatcgtcg ccaccagcac ctggagcagg gcgctgcgga tgcgcttgga
      361 gccgccgctg ccgagcgcca gctcgacgtc gcctccgggg gagaggacga aggtcggtga
      421 catcatcgat gccacgcgga cgcccggggg agcggcgtgg aagccgtcgg ggtggaggtc
      481 gtcttcgccc agcatgttgt tgcagcagat gccggtgccg ctgatgacgt cgcccgagca
      541 ctcgccgttg gaggtggtca tggcggcgac gttgccctca gcatcggcca cggtgacgtg
      601 cgtcgtgccc cgggtcatga gcgggcggga gccgggatcg tcgccgaggt cgccctggag
      661 cgcggcgatc acctcggcgt ggccgtcggc gcggtcgcgg tccacctcgg ccatgatccg
      721 ggccagctgc accgcccgct ccggcgaccc ggccggcagt ggcggcccag cggcctcgat
      781 gcggcgcagg gcgagcgcca gcagcgcccc gccgaaggtg ggcggcgggt tggtgaggag
      841 cgtccggtct cggtagcggg cacgcagcgg cacccgctcg atgacccggt acgacgccag
      901 gtccgccgcc gtgatcaggc cgccgtcgac cgagagctgg tccacgaggc gggcggcgag
      961 ctccccggcg tagaacgtct cgccgtcgcc gcggcccagg gcgtcgagga aggcggccag
     1021 gtccgggttg cgcagcgtgt cgccagcctg gagcaggcgg ccgtcgggga agaagacagc
     1081 ccggctggct tcggtgcggg cgaggatcgg ggcgaggagc gtgaagtcgc tgctctgcgt
     1141 cgccgtcacc tcgacgccgc ccgcggccag cgccacggcc ggggcgacga ggtcggccag
     1201 ggggaggcgc ccgaggcgct cgtgcacgtg caggtagccg gcgagcacgc ccgggacggc
     1261 caccgatccc ggtccgcagt ggaaggtctg ggtggccgag ccgaagtcga ccgagacctc
     1321 gtcgaacgcc ggcgcgaccg cgccggcgcc gagtccgcgt ccgggcgtgt cgacgaagaa
     1381 gtcgaacagc acgtcgccca gctccggcga gcgggcgagc aggaagccgc cgcccgccag
     1441 gctcgtgaag ccgggctcgc agaccgacga ggcgaacccg gccgcgatga tggcgtcgaa
     1501 cgcgttcccg ccccgctcca ggatcgacgc ggccgcgtcg gcggcccgga cgtggccggc
     1561 ggcgatgatc ccgttggtgc tgcggccgct catcggcggc gatggtacgg cgggtcgcgc
     1621 accgcggccg aacctcgtcc cgacggcgtc gttcccggtg cccagcggta cggcgttcga
     1681 ggcgacgggt caggcggtca ccacggcgtt gatggcagcg gccaccacgt tgccggtgtt
     1741 ggccatgccc ttggcgttcg ggtggaccgg tgcggcgtcg gtggtgggga cgatggcctc
     1801 gacccacttg ctcgacgagc acatgtcgtg gccgatgctc gaggtggcgg tgtcgacgta
     1861 gtgcacgccg ttggcctggg cgcgggtctt gagcatggtg ttgaggccct tggccacgcc
     1921 gcgcaggtag gcgatgtcac cggcgacgat cggcaccgtg ggccagcagc cgctgcccgt
     1981 ctcggggatg atcgtcgggt agccgaccac gaagatcttg gcgcccggcg cccgcttctt
     2041 ggcctcgacg agcacctggt cgatcttcgg ggcggtggcg gcgatcttct cggccagcag
     2101 gtcgtgcccg cccgtcacgt agtcgccctt gcagccggcg gagaacgggt cgagcgtggc
     2161 gcagttcttc acgatgccgc tgaagccgat gtcgttgccg ccgatgccga tcgtgacgac
     2221 cttggtgttg gcgttgagcg agttgaactg cggctggttc ggcccgggcg tcacgccttg
     2281 ctcgttgaag aagtgggtgg tggtggcgcc ggagcagctg acgtctttga acttggccgc
     2341 tttgatcttg gtcctggcga tgctcgggta gttcttcgtc gagcgcaggc agccgagcgg
     2401 gtcc
//
LOCUS       DATMFS010000585         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_66040, whole genome shotgun sequence.
ACCESSION   DATMFS010000585 DATMFS010000000
VERSION     DATMFS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66040"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VNQ33_11150"
     CDS             complement(<1..298)
                     /locus_tag="VNQ33_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98707.1"
                     /translation="MLRARTDDEAFAEVRREQARAEYATWNLVLNHFERATKLVDAAD
                     QPSISKQHGRSAVVLDMAQQLAISEQRAWGILHDARTLRDHTPRVWATFRVGHID"
     gene            complement(369..1220)
                     /locus_tag="VNQ33_11155"
     CDS             complement(369..1220)
                     /locus_tag="VNQ33_11155"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HWJ98708.1"
                     /translation="MDLSPAQLSALVAIADTGSFEAAAERLHVTPSAVSQRIRALESA
                     VGRVLVGRGTPCVPTEAGAPLVRLGRQLELVFAEALTDGLTEGPTDLALAINADSLAT
                     WFRPVLAEIASWEGVALRLRVEDEGHSHDLLRRGEVVAAVTSDPSPVQGCSVTPLGTM
                     RYLPVAHPSLLGRSGRPDWAGMPMVVFNAKDRLQHEELQRHTEAAPAVVHEVPSSDDF
                     RAAVEAGLGWGLLPEVQARDGIEAGTLVRLGRSVTRVSLYWQRWRLDSELLDRLSDAV
                     AAHAPAR"
     gene            1171..1896
                     /locus_tag="VNQ33_11160"
     CDS             1171..1896
                     /locus_tag="VNQ33_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HWJ98709.1"
                     /translation="MSAIATSADSWAGDRSMDRVKHSFTMQRKIRWTSRADRPYGRTV
                     LNAFMTGMITGLSLIVVIGAQNAFVLRQGIRRSHVAAVVAICAVSDLALILAGVAGIG
                     AIIDRAGWLIDVVTWLGVAFLVWYGIGSIRRAFRPEHLDAAGRTAATLRAVVLQVLAL
                     TWLNPHVYLDTVVLLGSIAQTHGDDRWWFGAGASVGSIVWFSSLGFGATRLAPLLARP
                     RAWQVLDLVIGLVMFVIAASLVW"
     gene            1926..>2775
                     /locus_tag="VNQ33_11165"
     CDS             1926..>2775
                     /locus_tag="VNQ33_11165"
                     /inference="COORDINATES: protein motif:HMM:NF005928.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWJ98710.1"
                     /translation="MDPVEALRETAFWLERERASTYRVEAFRKAAAALADLAPDEVAE
                     RARSGSLGRMKGLGPRTTTVVEQALSGDVPEYLSDLRARNTGPLAEAGAELRALLRGD
                     LHSHSDWSDGGSPIEEMARAAVWRGREYQAITDHSPRLTVANGLSRERLEVQLEVVAD
                     VNDQFPDVTLLTGIEVDILDDGALDQDDDLLGRLDVVVASVHSKLRMDAPAMTRRMVA
                     AVSNPHTDVLGHCTGRLVEGSRGTRKQSQFDPERVFRACAEHGVAVEINSRPERQDPP
                     DELIDLA"
BASE COUNT          372 a          985 c         1007 g          411 t
ORIGIN      
        1 cgtcgatgtg accgacccgg aacgtggccc agacgcgagg cgtgtggtcg cgcagggtgc
       61 gggcgtcgtg caggatgccc cacgcccgct gctcggagat cgccagctgc tgggccatgt
      121 cgagcaccac ggcgctgcgg ccgtgctgct tggagatcga cggctggtcg gctgcgtcga
      181 cgagcttcgt cgcgcgctcg aagtggttca ggacgagatt ccaggtggcg tactcggcgc
      241 gggcctgctc gcgtcggact tcggcgaatg cctcgtcgtc ggtgcgggcc ctgagcatgt
      301 ctccatcata gaacgtgtgt tcgatgaatc ggtcgttcgt gccgctcttc cagcgatgtg
      361 agacggtatc atcgagccgg agcgtgggcg gccacggcgt cgctgagccg gtcgagcagc
      421 tcggagtcga ggcgccagcg ctgccagtac aaggacacgc gcgtgaccga ccggcccagg
      481 cggaccagcg tgcccgcctc gatgccatcg cgggcctgca cctcggggag caggccccag
      541 ccgagccctg cctcgaccgc ggcccggaag tcgtcggacg agggcacctc gtggacgacg
      601 gccggcgcgg cctcggtgtg gcgctgcagc tcctcgtgct ggaggcggtc cttcgcgttg
      661 aacacgacca tcggcatgcc cgcccagtcg ggccgtccgg accggccgag cagcgacggg
      721 tgtgccaccg gcaggtatcg catggtgccg agcggcgtca cggagcagcc ctgcacgggg
      781 gaggggtcgc tcgtcaccgc ggcgaccacc tcgccgcgcc tcaggaggtc gtggctgtgg
      841 ccctcgtcct ccacgcgcag ccgcagcgcg acgccctccc atgaggcgat ctcggccagc
      901 acgggccgga accacgtggc cagggagtcg gcgttgatcg ccagggcgag gtccgtcgga
      961 ccctcggtca gtccgtccgt cagcgcctcg gcgaagacga gctcgagctg gcgaccgagc
     1021 cggacgagcg gggctcccgc ctcggtcggc acgcagggcg tcccgcggcc caccaggacc
     1081 cggccgacgg cgctctcgag ggcgcggatg cgctggctga cggcgctcgg cgtgacgtgg
     1141 agccgctcgg ccgcggcctc gaaggagccc gtgtcggcga tcgccaccag cgccgacagc
     1201 tgggcggggg acaggtccat ggaccgagtt aagcactcct tcaccatgca aagaaagatt
     1261 cgttggactt cacgagccga ccgaccctac ggtcgaacgg tgctgaacgc cttcatgacc
     1321 gggatgatca ccggcctgtc cctcatcgtc gtcatcggcg cccagaacgc cttcgtcctg
     1381 cggcagggca tccgccggtc ccacgtggcg gccgtcgtcg ccatctgcgc ggtgtcggac
     1441 ctcgcgctca tcctggccgg ggtcgcgggg atcggggcga tcatcgaccg cgcgggctgg
     1501 ctcatcgacg tcgtcacgtg gctcggcgtg gccttcctcg tctggtacgg catcggctcg
     1561 atccgccgcg cgttccgccc cgagcacctc gacgcggccg ggcgcaccgc cgccaccctg
     1621 cgcgccgtgg tgttgcaggt gctggcgctc acgtggctca acccgcacgt ctacctcgac
     1681 acggtggtgc tgctcggctc gatcgcccag acgcacggtg acgatcgttg gtggttcggc
     1741 gcgggcgcct cggtcggcag catcgtgtgg ttctcgtcgc tgggattcgg ggccacgcgc
     1801 ctcgccccgc tgctcgcgcg accgcgggcc tggcaggtgc tcgacctggt gatcggtctc
     1861 gtgatgttcg tgatcgcggc ttcactggtc tggtgactcg cggggacatc ccgcacgatg
     1921 gaggggtgga ccccgtcgag gcgctgcgcg agaccgcgtt ctggctcgag cgcgagcgtg
     1981 cgtcgaccta ccgggtggag gccttccgca aggccgccgc ggcgctcgcc gacctggccc
     2041 ccgacgaggt cgccgagcgc gcccggagcg gatcgctggg ccggatgaag ggtctcgggc
     2101 cgcggaccac caccgtggtc gagcaggcgc tctcgggcga cgtgccggag tacctgtccg
     2161 atctgcgggc ccgcaacacc ggccctctcg ccgaggccgg ggccgagctg cgtgccctgc
     2221 tccgtggcga cctgcacagc cactccgact ggtccgacgg cggcagcccg atcgaggaga
     2281 tggcgcgggc ggcggtctgg cggggccgcg agtaccaggc catcaccgac cactcgccgc
     2341 ggctcacggt ggccaacggg ctgagccgcg agcgcctcga ggtgcagctc gaggtcgtgg
     2401 ccgacgtgaa cgaccagttc cccgacgtca ccctgctgac gggcatcgag gtcgacatcc
     2461 tcgacgacgg agcgctggac caggacgacg acctgctcgg ccgcctcgac gtggtcgtcg
     2521 cgagcgtgca ctcgaagctg cgcatggacg cgccggcgat gacccgacgc atggtggccg
     2581 cggtctcgaa tccacacacc gacgtgctgg ggcactgcac aggccgcctc gtcgagggct
     2641 cgcgcggcac gcgcaagcag tcccagttcg atcccgagcg ggtcttccgc gcctgcgccg
     2701 agcacggcgt cgccgtcgag atcaactcgc gtcccgagcg ccaggacccg ccggacgagc
     2761 tgatcgacct cgcgt
//
LOCUS       DATMFS010000586         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_250983, whole genome shotgun sequence.
ACCESSION   DATMFS010000586 DATMFS010000000
VERSION     DATMFS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250983"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VNQ33_11170"
     CDS             <1..361
                     /locus_tag="VNQ33_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ98711.1"
                     /translation="PTPASVAVARRWSMDVLDRAGAAELADTMALLVSELVSNVVLHA
                     RTPCSLSILRTSDCFRVEVQDGSDRLPGLRERADPLAQSGRGMQLVDGLSAAHGVDPE
                     PRGGKRVWFELKVPEVP"
     gene            358..828
                     /locus_tag="VNQ33_11175"
     CDS             358..828
                     /locus_tag="VNQ33_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98712.1"
                     /translation="MTDDAERRPIVLEGLPEAAFRSAVGYLDDALRECQLVLVAEGQG
                     HETDAELSRVARSLVPDLEEVGDAFRAAGSQVRDDGTLRLAGALTVDQAALVADIQVL
                     LVHLRILGRRGDLLLESDPEIAQLLTWVWEEVSDQLAGRPPRPYRRASPPDSTA"
     gene            876..2408
                     /locus_tag="VNQ33_11180"
     CDS             876..2408
                     /locus_tag="VNQ33_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWJ98713.1"
                     /translation="MAAGNVLYITVDQWRGDCLGVAGHPVVRTPNLDRLAARGVRFAN
                     HWSVTAPCGPSRASLHTGLYLMNHRSVNNGTPLDHRFANIAREARAVGYDPTLFGYTD
                     TSVDPRTVTDPQDPRLFDYEGLLPGFTPGTLLGWHAMEPWLDWLAARGHQIPGTWEEL
                     FEPIPGYPGAEEHGRTWAPSIFPAEHTETAFMTERFLEWHDERPDGDPWFAHVSYIRP
                     HPPYRAPEGYHDLYDPDDGPAFRRLGDRAAELAMHPIHAAGAHITKAPVDEREMRQLR
                     ATYWGNMTEVDDQLGRLLDELERRGDLDDTLVVLTADHGEMLGDHWLIEKLGWWDESY
                     HVPLIVVDPRPDADATRGTAVERFTESVDVMPTICTWMGAEVPIQVDGRELQPFLHDG
                     EAPSDWRTEAHWQWDFRDPVDHVAEDLLGLTMEQCTLDVIRGDDYKYVQFGNGDRLFF
                     DFQDDPDQVVDRADDPAVLPALADAQSRLLTWRMRHDDRTLTGSLVTAAHGLVVRRDP
                     RR"
     gene            2549..2821
                     /locus_tag="VNQ33_11185"
     CDS             2549..2821
                     /locus_tag="VNQ33_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98714.1"
                     /translation="MAERARFWRANRWREALRPDSDLAEAEGLAAGTGLLTGTVARSV
                     GVSKVSVANPDCPSCAGQSEIVVVDLIVHATTLRCQSCGRRWTLQT"
     gene            2896..3249
                     /locus_tag="VNQ33_11190"
     CDS             2896..3249
                     /locus_tag="VNQ33_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98715.1"
                     /translation="MAKGRATDQARPDAAPAGALETVGPPLLLLALGFVCVVASLPLV
                     AADGIPGHVVGYVVGALIPILIIGAVRRVDLSRRRSPYYVPRSFLQPALFALAISAVV
                     VAGLQIWPIATELAS"
BASE COUNT          430 a         1237 c         1135 g          453 t
ORIGIN      
        1 gcccacgccg gcgagcgtcg ccgttgcccg gaggtggagc atggacgtgc tcgatcgtgc
       61 cggcgcggcc gagctcgccg acacgatggc cctgctcgtg agcgagctgg tgagcaacgt
      121 cgtcttgcac gcccggacgc cgtgctcgct gtcgatcctc cggacgagcg actgcttccg
      181 cgtcgaggtc caagacggca gcgaccgcct tcccggcctg cgcgagcgcg ccgacccgct
      241 cgcccagtcc ggccgcggca tgcagctcgt cgacgggctc agcgcggcgc acggggtcga
      301 tcccgagcct cggggcggca agcgggtctg gttcgagctc aaggtgcccg aggtcccgtg
      361 accgacgatg cggagcgccg cccgatcgtc ctcgaaggac ttcccgaggc cgcgttccgc
      421 tccgcggtcg gctatctcga cgatgccctg cgcgagtgcc agctggtgct ggtggccgag
      481 ggccagggcc acgagaccga tgccgagctc agccgggtgg cccgctcgct cgttcccgac
      541 ctcgaggagg tcggcgacgc gttccgagct gccggctccc aggtccgcga cgacgggacc
      601 ctccgtctgg ctggtgcgct gacggtcgac caggcggcgc tcgtcgccga catccaggtg
      661 ctgctcgtgc acctgcggat cctcggccgc cggggcgacc tgctcctcga gagcgatccc
      721 gagatcgccc agctgctgac ctgggtctgg gaagaggtct cggaccagct cgccggccgc
      781 ccgccccggc cgtaccgccg ggcctcgccg ccggactcca ccgcatagcg tcgcggccgc
      841 gcaccgtgcg catgcgacag cgagagggag gccgggtggc cgccggaaac gtgctctaca
      901 tcaccgtcga ccagtggcgg ggggattgcc tgggcgtggc cggtcacccg gtggtgcgga
      961 cgccgaacct cgaccgcctg gcggcccgtg gcgtccgctt cgccaaccac tggtcggtca
     1021 cggcgccgtg cgggccgagc cgggcctccc tgcacaccgg gctctacctg atgaaccacc
     1081 gttccgtgaa caacgggacg ccgctcgacc accggttcgc caacatcgcg cgggaggcgc
     1141 gcgccgtcgg gtacgacccg acgctgttcg gctacacgga cacgagcgtc gatccgcgga
     1201 ccgtcaccga tccgcaggac ccgcggctgt tcgactacga gggcctcctg cccggcttca
     1261 ccccggggac gctgctcggc tggcacgcca tggagccgtg gctcgactgg ctggcggcgc
     1321 gcggccacca gatcccgggg acgtgggagg agctcttcga gccgatcccg ggctatcccg
     1381 gagccgagga gcacggccgc acctgggcgc cgtcgatctt ccccgccgag cacaccgaga
     1441 cggccttcat gacggagcgc ttcctcgagt ggcacgacga gcggcccgac ggcgacccct
     1501 ggttcgccca cgtgtcctac atccgacccc acccgccgta ccgggcgccg gagggctacc
     1561 acgacctcta cgaccccgac gacggtccgg ccttccggcg cctcggcgac cgggcggccg
     1621 agctggccat gcaccccatc cacgccgccg gcgctcacat caccaaggcg cccgtcgacg
     1681 agcgggagat gcgccagctg cgcgccacgt actgggggaa catgaccgag gtcgacgacc
     1741 agctgggccg gctgctcgac gagctcgagc ggcgcggcga cctcgatgac acgctggtcg
     1801 tcctcaccgc cgaccacggc gagatgctgg gcgaccactg gctcatcgag aagctgggct
     1861 ggtgggacga gagctaccac gtgccgctga tcgtggtgga tccccgcccc gacgccgatg
     1921 ccacccgcgg cacggccgtc gagcggttca cggagtcggt cgacgtcatg cccaccatct
     1981 gcacctggat gggcgccgag gtcccgatcc aggtcgacgg ccgcgagctg cagccgttcc
     2041 tccacgacgg cgaggcgccg tcggactggc ggaccgaagc ccactggcag tgggacttcc
     2101 gcgacccggt cgaccacgtc gctgaggacc tcctcggcct caccatggag cagtgcaccc
     2161 tcgacgtgat ccgcggcgac gactacaagt acgtccagtt cggcaacggc gaccggctgt
     2221 tcttcgactt ccaggacgac ccggatcagg tggtggaccg cgccgacgac ccggcggtgc
     2281 tgcccgccct cgccgacgcc cagtcccgcc tgctgacgtg gcggatgcgc cacgacgacc
     2341 gcaccctcac gggcagcctc gtcaccgccg cgcacggtct ggtcgtgcgc cgggaccccc
     2401 gccgctgaac cgcgccgcga cgggccggcc agcggtgccg agcggtgcgc cggggcgcca
     2461 tcgtgtccgg atcgggccac ggtcgtgtcc gaattgggtc agatggcccg ttccgcgtgg
     2521 agggctcgtc gcgtaacctt cccccggcat ggccgagcga gcgcgattct ggcgggcgaa
     2581 ccggtggcgc gaggcgctgc gccccgattc ggacctggcc gaagccgaag ggctggcggc
     2641 cggcaccggc ctgctcacgg gcaccgtcgc caggtccgtc ggcgtctcca aggtctccgt
     2701 ggccaacccg gactgcccct cgtgcgccgg ccagagcgag atcgtcgtgg tggacctgat
     2761 cgtgcacgcc accaccctcc gctgccagtc ctgcggccgg cgctggaccc tgcagacctg
     2821 agccgctccg gcagccagcg gtcacgggtg acccgctagg gtctgcggcg gatcacagcc
     2881 acctgggggt accgcatggc gaaggggcga gcaacggacc aggcgcgacc agacgccgct
     2941 ccggccggtg cgctggagac cgtcgggccg ccgctgctgc tcttggccct gggcttcgtc
     3001 tgcgtggtgg ccagcctgcc gctggtcgcc gccgacggca tcccgggcca cgtcgtcggc
     3061 tacgtggtcg gcgcgctgat cccgatcctc atcatcggcg ccgtccgccg ggtcgatctg
     3121 agccgtcgcc ggtcgccgta ctacgtcccc cgttcgttcc tccagccggc gctgttcgcg
     3181 ctggccatct ccgccgtcgt cgtcgccggc ctgcagatct ggcccatcgc cacggagctg
     3241 gcctcgtgac ccgcc
//
LOCUS       DATMFS010000587         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_20528, whole genome shotgun sequence.
ACCESSION   DATMFS010000587 DATMFS010000000
VERSION     DATMFS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20528"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1075)
                     /locus_tag="VNQ33_11195"
     CDS             complement(116..1075)
                     /locus_tag="VNQ33_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98716.1"
                     /translation="MLLRDLVEAVDALDIDAHVDGEALVIDGSRFIPGIVVRAHPTPA
                     DLSQLIAEAPGKVPAVVVADRISDAGRTVLRRAGWGWLDRRGHMRLWAPGIRIESPLP
                     GEAATAGRRGPVNAWTTVGLEIALASLCEPNEPVTARAVAAKIGRSVGATHELIGRFV
                     ELGLIGRQTHLPLLPDLFWEATARWPDDGWIGLPVPLEDVAERIGAEQLVRVDERAAT
                     LGGARIAAAGDLPPRCYVRAASAFRRARSLGDRGTRDAERPVRCWVRAAPVTWLPLND
                     EHPADAAHPWSVGHPQLCALRLAADPARGREIVEDWGIVPHPL"
     gene            complement(1180..1842)
                     /locus_tag="VNQ33_11200"
     CDS             complement(1180..1842)
                     /locus_tag="VNQ33_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98717.1"
                     /translation="MADRPSQGCLVPLALIAVVVVGTLIGLKVLPDGPEPDVFIIGDS
                     VTNMSEEAISDQFVHTPLEFVAYPGYSSTMLLPDVLDSMGAPGDPAHARQRVAVLVGY
                     NDVRLREVDTPSLRKMVDLTAEFECGVWLTLPARPGGKDNPNAMADSPLVDEWNLRLQ
                     AEVERHDNLHLSDAWAQAVNNAPVGSILRKDNVHPNAAGRLLLARIYREALDQSCPKE
                     RS"
     gene            complement(1852..2244)
                     /locus_tag="VNQ33_11205"
     CDS             complement(1852..2244)
                     /locus_tag="VNQ33_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98718.1"
                     /translation="MGDEIEAAAGDDVEVEVETDVTVDVYDADGDGQADTVVVTETSV
                     VDVDGDGVPDIVQQVTTTAIDVDGDGVPDIVDRVTTTAIDVDGDGEIDIIDEVTETGV
                     DVDGDGEIDAADVTEATYVRDDEGSAES"
     gene            complement(2289..2768)
                     /locus_tag="VNQ33_11210"
     CDS             complement(2289..2768)
                     /locus_tag="VNQ33_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98719.1"
                     /translation="MRADPAASPPDRRTEAGLGFDPEAGRLALGSESVVVPPGASNRA
                     VLLAYVDLTAKRRNRSVAEVIEVRQADIDALAAALDLDAADLGRQIEEVLGATRAEAI
                     RLVSRLRESRLIGGITKAATGAAVAGAILAGAGAVIAGDGTLLVPPAPLDPEDPAGS"
     gene            2923..>3177
                     /locus_tag="VNQ33_11215"
     CDS             2923..>3177
                     /locus_tag="VNQ33_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HWJ98720.1"
                     /translation="MTATSSPAADPLDREALRAKYRLERDKRLRPDGNDQYLEPTGRF
                     ADLLEDPYTDAAEREPVHDHVTVAFIGGGFAGLVTGARLKE"
BASE COUNT          439 a         1148 c         1175 g          415 t
ORIGIN      
        1 cacatgacgg ccaagccgcc gatgaggcgc agctcgggcg gaagcgacgc gagcagcgcc
       61 acctcacggt gggtcgtccc gccttcgcgg cccgacagga ggacgggcga ggcggtcaca
      121 gcgggtgggg cacgatgccc cagtcctcga cgatctcccg gccgcgggct gggtcggcgg
      181 cgaggcgcag ggcgcacagc tgcgggtggc ccaccgacca ggggtgggcg gcgtcggccg
      241 ggtgctcgtc gttcaggggc agccaggtga ccggggcggc ccgcacccag cagcgcaccg
      301 ggcgctcggc atcgcgcgtc ccgcggtcgc ccagcgagcg ggcccggcgg aacgccgacg
      361 cggcgcgcac gtagcagcgc ggcggcaggt cccccgctgc cgcgatgcgg gcgcctccga
      421 gggtggccgc ccgctcgtcg acacggacga gctgctcggc tccgatgcgc tcggcgacgt
      481 cctccagcgg gaccggcagc ccgatccacc cgtcgtcggg ccagcgcgcc gtcgcctccc
      541 agaagaggtc gggcagcagg gggaggtggg tctggcgccc gatgagcccc agctccacga
      601 accgtccgat gagctcgtgg gtggcaccga cgctgcggcc gatcttggcg gcgacggccc
      661 gagcggtgac gggctcgttc ggctcgcaga gcgacgccag cgcgatctcc aggccaacgg
      721 tggtccaggc gttgaccggg cctcgacgcc cggcggtggc cgcttcgccc gggagcggcg
      781 actcgatgcg gatgcccggc gcccacagcc gcatatggcc gcggcggtcg agccaccccc
      841 agccggcacg tcgcaagacg gtgcggccgg cgtcactgat gcggtcggcg accaccacgg
      901 ccgggacctt gcccggcgcc tcggcgatga gctggctgag gtcggccggg gtgggatggg
      961 cccgcacgac gatccccggg atgaagcggc tcccgtcgat gaccagggcc tcgccgtcga
     1021 cgtgggcatc gatgtcgagg gcgtcgacgg cctcgacgag gtcgcggagc agcaatccct
     1081 gttcctcggt acgggccgtc acgaacacag aacaaaccct acggcggcct gcgtgccaac
     1141 gcaacgttcg gcgttcgtga cggcacgaac gccgagtggc tacgagcgtt ccttcgggca
     1201 gctctggtcg agcgcctcgc ggtagatgcg ggccaggagc agccggcccg ccgcgttcgg
     1261 gtgcacgttg tccttgcgga ggatcgaccc caccggcgcg ttgttcaccg cctgggccca
     1321 cgcgtcgctc aggtggaggt tgtcgtggcg ctcgacttcg gcctggaggc gcaggttcca
     1381 ctcgtcgacc agcggcgagt cggccatggc gttcgggttg tccttcccgc ccgggcgcgc
     1441 cggcagcgtg agccacacgc cgcactcgaa ctcggcggtc aggtcgacca tcttccgcag
     1501 cgacggggtg tcgacctcgc gcaggcggac gtcgttgtac ccgaccagca cggccacccg
     1561 ctgccgggcg tgcgcgggat cgccgggtgc gcccatcgag tcgagcacgt ccggcagcag
     1621 catcgtcgac gagtagcccg ggtacgcgac gaactcgagc ggcgtgtgga cgaactggtc
     1681 cgagatggcc tcctcggaca tgttggtgac cgagtcgccg atgatgaaga catcgggctc
     1741 aggcccgtcg ggcaggacct tcagcccgat gagggtcccg acgaccacga ccgcgatcag
     1801 cgcgaggggg accaagcagc cctgcgacgg tcggtcagcc atggcgcagg ctcaggactc
     1861 cgcagatccc tcgtcgtcgc ggacgtaggt ggcctcggtg acgtccgccg cgtcgatctc
     1921 gccgtcgccg tccacatcga cgcccgtctc ggtgacctcg tcgatgatgt cgatctcgcc
     1981 gtcgccgtcg acgtcgatgg ccgtcgtggt gacccggtcg acgatgtcgg gcacgccgtc
     2041 gccgtccacg tcgatggccg tcgtggtgac ctgctgcacg atgtcgggca cgccgtcgcc
     2101 gtcgacatcg accacgctgg tctccgtcac gacgacggtg tcggcctggc cgtcgccgtc
     2161 cgcgtcgtac acgtcgacgg tgacgtcggt ctcgacctcg acctcgacgt cgtcgcccgc
     2221 agccgcttcg atctcatcgc ccatggtggg tgctcccgct cagatggtgg acggggcatc
     2281 ttggcacgtc aggacccggc cggatcttca gggtccaggg gcgcgggcgg gaccagcagg
     2341 gtgccgtcgc cggcgatcac cgcgccggca cccgcgagga tcgccccagc gacggcggcg
     2401 ccggtggccg ctttggtgat gccgccgatg agccgcgact cgcgcagccg ggacacgagg
     2461 cggatggcct cggcccgggt ggcgccgagc acctcctcga tctgccggcc caggtcggcg
     2521 gcatcgaggt cgagggcggc ggccagggcg tcgatgtcgg cctgacggac ctcgatgacc
     2581 tcggcgacgc tgcggttccg gcgcttggcc gtcagatcca cgtacgccag caggaccgcc
     2641 cggttgctgg caccgggcgg gaccaccacc gactcggagc cgagggccag gcgacccgcc
     2701 tccgggtcga acccgaggcc cgcctcggtc cggcggtcgg ggggcgaagc agcagggtcg
     2761 gcgcgcatga gcgaagcatc ggcagcagcg gcccggaact gagcgcaccg gctccagatg
     2821 ggccgtcagg cccgggagga ccgcggcccg tcctcgggcg cgccgcgcct cccggccgct
     2881 ggcgcgctgg cgaggcccag ccgcccatgg gaaacttgca cggtgactgc aacaagctcc
     2941 ccagccgcgg accccctcga ccgggaggcg ctgcgcgcca agtaccggct cgagcgggac
     3001 aagcggctgc gccccgatgg caacgaccag tacctcgagc ccacggggcg cttcgccgac
     3061 ctgctcgaag atccctacac cgacgccgcc gagcgggagc cggtccacga ccacgtgacc
     3121 gtcgcgttca tcggcggcgg cttcgccggg ctcgtcaccg gcgcccggct caaagag
//
LOCUS       DATMFS010000588         5883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_205829, whole genome shotgun sequence.
ACCESSION   DATMFS010000588 DATMFS010000000
VERSION     DATMFS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205829"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..623)
                     /locus_tag="VNQ33_11220"
     CDS             complement(72..623)
                     /locus_tag="VNQ33_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWJ98721.1"
                     /translation="MTPDPADASGAAHPPAVVVSVTEIRRRPGSRMPVERTLVADGLG
                     LTDVAIPDGAEITFTGELESIHEGVVLTGAADVPWTGECRRCLKELHGVATIDIREVY
                     ETKPTDGETWPLDHDQVDLGPLLHDIALLALPLAPLCTDACMGPVPEVFPAVVEGDAV
                     PGEAPEEAPAKDPRWAALDDLDL"
     gene            complement(620..1309)
                     /locus_tag="VNQ33_11225"
     CDS             complement(620..1309)
                     /locus_tag="VNQ33_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98722.1"
                     /translation="MSYDDRPYDERGYDDRQPHDPRDYDERAYADQPFDAEAEREPVD
                     EPQTRRTLVRGRTQVDAEELLRRVSDLIAAARPMPLSASAMINKEEVLELLDEAIGRL
                     PDELREARWLRKEREEYLAKMRADGDEITDAARARAEQMVQRTEVVKAAEHRARRIVD
                     GAEAEARRLRLECEDFCDQKLASFEIVLERTLKLVGSGREKLQATNLTRPEAEPAPEP
                     EDEGFFDQDRS"
     gene            complement(1306..1794)
                     /gene="coaD"
                     /locus_tag="VNQ33_11230"
     CDS             complement(1306..1794)
                     /gene="coaD"
                     /locus_tag="VNQ33_11230"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWJ98723.1"
                     /translation="MTRVLYPGSFDPIHNGHLELIEVASRLFDSVVVATIGNPQKTTG
                     LFSLEERREMIAESVAHLPNVEVTVYSNLVVNLAKEVEADFLVKGLRAVSDFESELQQ
                     SQMNHAISGVHTVFIPSATEHSFVASKWIREIARFGGDVSSMVPAPVAARLAESFQEP
                     SA"
     gene            complement(1791..2492)
                     /gene="rsmD"
                     /locus_tag="VNQ33_11235"
     CDS             complement(1791..2492)
                     /gene="rsmD"
                     /locus_tag="VNQ33_11235"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWJ98724.1"
                     /translation="MARIAPRPQPRGSPLAVGRERPPRGRGAVSGRKPTPRPRRSAGS
                     ARPAPSKGALRIVAGRAGGRRIDAPPGSGTRPTSDRVREAVFNALDSLDAIEGARVLD
                     AFAGSGALGIEALSRGAAHATFFDTDPQAVEIATRNLSTLGLAADATVVARAAERSLA
                     PGGPFDLVLLDPPYAYDGWDDLLVAVARALSADGVVVIESDREVPLPAGLVGIKSKAY
                     GGTVVQFATVAGAPS"
     gene            complement(2405..3289)
                     /locus_tag="VNQ33_11240"
     CDS             complement(2405..3289)
                     /locus_tag="VNQ33_11240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98725.1"
                     /translation="MGAKQREDLSGKVVIVTGANSGIGKETAVALAAMGATVVMTART
                     PSKGEEARTEVLARSGSDRVVLGRLDLASFASIREFASWFLAEFDRLDVLVNNAGLMV
                     DTRQETADGFELMFGTNHLGHFLLTDLLRQRLIRSAPSRVIVLSSVAHRWALKGLDRG
                     DLQGRVRFGGGRNYGLSKLANAQFALELSRQLAADGVTVYAVHPGSIYSGFGVDGDTG
                     ALSWWITTFGRFVLRSPKAGAKTQVLLASSSKPRITARSGAYWSHGRRWRASRHARNR
                     EEARWLWDESARLVAEVP"
     gene            3360..4115
                     /locus_tag="VNQ33_11245"
     CDS             3360..4115
                     /locus_tag="VNQ33_11245"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWJ98726.1"
                     /translation="MSEPTDPSPGDAVRIVRIDRPERRNAVNSETAERLYREFQAFDS
                     DPTAKVAILTGDTSSFCAGADLTDLPRLRESGPMGPTRMALSKPVIAAVEGWCVAGGV
                     ELAAWCDIRIAGAGAKFGCLERRWGVPLIDGGTIRLPRIVGLGRAMDLMLTGRGFDAA
                     EAERIGFVSRLVPEGTALDAAIEAAEVIASSPWPAVVHDRLSAYESYGLSFEEALANE
                     DRHGRAVIFDPGFADGVAQFQANQAKRRTEGDR"
     gene            4112..4360
                     /locus_tag="VNQ33_11250"
     CDS             4112..4360
                     /locus_tag="VNQ33_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98727.1"
                     /translation="MSDHRADDDHDEGPTDSSGAPLVPQTIVCVDCGSTAHLMTRPDD
                     TGRFWPGDLVSYRCEDCMDRWDLIVPGDDPEDDHGPGW"
     gene            complement(4376..4555)
                     /locus_tag="VNQ33_11255"
     CDS             complement(4376..4555)
                     /locus_tag="VNQ33_11255"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HWJ98728.1"
                     /translation="MLEMRAVCERCGTALPPGSDQARICSYECTFCAGCVDGPLAGAC
                     PNCGGELQPRPRRAA"
     gene            complement(4576..4971)
                     /locus_tag="VNQ33_11260"
     CDS             complement(4576..4971)
                     /locus_tag="VNQ33_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWJ98729.1"
                     /translation="MTLDADLASLLAGKNFAAFTTLMADGRPQTQIMWIGDDGEHLLI
                     NTEIHRQKFKNAERDPRVTVTVFNSENAYQYVEARGRVVGTVGGDEARAHIDALAEKY
                     MGVDRYPNPIQSERVVLQIAIDKLHKNGY"
     gene            5097..>5883
                     /locus_tag="VNQ33_11265"
     CDS             5097..>5883
                     /locus_tag="VNQ33_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ98730.1"
                     /translation="MRFSFWISTQLSWTETLDLASHAEATGWDGLWVADHFMPNDDTA
                     LDQPMHEALGLLGALAAAVPRVRLGSLVLGNTYRHPAVVAKTAATIDHISGGRLVLGV
                     GAGWQENEHARYGIPLGTVRERVDWFEEACQVLVGLRDEARTTVAGARYQLDDAPAEP
                     KPTGPLPLLIGASGQKRMAAIVAEYADEWNTWSTPSLWREKRIGFDQALDQAGRDRAD
                     LYRSTQALVFLGDDGYQTAEDFAAIRPAIGGTSEQLVATIGQWA"
BASE COUNT          804 a         2110 c         2139 g          830 t
ORIGIN      
        1 gccgaacttt tcgtgatcgt ggtggccctg ccaccaactt cacgaaaagt tcggatgtgt
       61 gggtgggcga ctcagaggtc gaggtcgtcg agggcggccc agcgggggtc cttggccggg
      121 gcctcctcgg gcgcttcgcc cgggaccgcg tcgccctcga ccacggcggg gaacacctcg
      181 gggacgggcc ccatgcaggc gtcggtgcac agcggcgcca gcggcagcgc caacagggcg
      241 atgtcgtgga gcagcggccc gaggtcgacc tggtcgtggt ccagcggcca cgtctcgccg
      301 tcggtgggct tggtctcgta cacctcgcgg atgtcgatgg tggccacgcc gtgcagctcc
      361 ttgaggcagc gccggcactc gcccgtccac ggcacgtccg cggcgccggt cagcacgacg
      421 ccctcgtgga tggactccag ctcaccggtg aaggtgatct cggcgccgtc ggggatggcg
      481 acgtcggtca ggcccaggcc atcggcgacg agggtccgct cgaccggcat ccggctgccg
      541 ggccgccggc ggatctcggt gaccgacacg accacggccg gcggatgggc cgccccggaa
      601 gcgtcggccg ggtccggggt cacgaccggt cctggtcgaa gaacccctcg tcttcgggct
      661 ccggagccgg ttcggcctcg ggccgggtga ggttcgtggc ctgcagcttc tcacggcccg
      721 agcccaccag cttcagcgtc cgctcgagga cgatctcgaa gctggcgagc ttctggtcgc
      781 agaagtcctc gcactccagg cgcaggcggc gggcctcggc ctcggcgccg tcgacgatgc
      841 ggcgggcgcg gtgctcggcc gccttcacga cctcggtgcg ctggaccatc tgctcggccc
      901 gggcccgggc ggcatcggtg atctcgtcgc cgtcggcgcg catcttggcg aggtactcct
      961 cgcgctcctt gcgcagccag cgggcctcgc gcagctcgtc ggggaggcgg ccgatggcct
     1021 cgtcgagcag ctcgaggacc tcttccttgt tgatcatcgc cgaggccgac agcggcatcg
     1081 ggcgggccgc cgcgatcagg tcggacaccc ggcggagcag ctcctcggca tcgacctggg
     1141 tgcggccgcg gacgagggtg cgccgggtct ggggctcgtc gaccggctcc cgctccgctt
     1201 cggcgtcgaa cggctggtcg gcgtaggccc gctcgtcgta gtcccgggga tcgtggggct
     1261 ggcggtcgtc gtacccgcgt tcgtcgtagg ggcggtcgtc gtagctcatg cggagggctc
     1321 ctggaacgat tcggcgagac gggcggcgac cggcgccggc accatcgagg acacgtcgcc
     1381 gccgaaccgg gcgatctccc ggatccactt ggaggcgacg aaggagtgct cggtggccga
     1441 cgggatgaac accgtgtgga ccccggagat ggcgtggttc atctgcgact gctgcagctc
     1501 ggactcgaag tccgacacgg cacggaggcc cttcaccaag aagtcggcct ccacctcctt
     1561 ggccaggttg accacgaggt tggagtacac ggtgacctcg acgttgggga ggtgggcgac
     1621 ggactcggcg atcatctccc ggcgctcctc gagcgagaac aggccggtgg tcttctgcgg
     1681 gttgccgatg gtggcgacca cgacggagtc gaacagccgc gacgcgacct cgatgagctc
     1741 gaggtgtccg ttgtggatgg ggtcgaacga ccccgggtag aggactcgcg tcacgaagga
     1801 gctccggcga cagtggcgaa ctggaccacc gtaccgccgt aggccttgga cttgatgcca
     1861 acgagacccg ccggcagcgg cacctcacgg tccgattcga tgacgacgac gccgtcagca
     1921 gacagcgccc tggccacggc gacgaggagg tcgtcccacc cgtcgtaggc gtacggcggg
     1981 tcgagcagga cgaggtcgaa ggggccgccc ggggcgagcg accgctcggc ggcccgagcc
     2041 acgacggtgg cgtcggcggc caggccgagg gtgctcaggt tgcgggtcgc gatctcgacc
     2101 gcctgcggat cggtgtcgaa gaaggtggcg tgcgccgcgc cccgggacag ggcctcgatg
     2161 ccgagggcac cgctcccggc gaaggcgtcg agcaccctcg caccctcgat cgcgtcgagg
     2221 ctgtcgaggg cgttgaacac cgcctcgcgc acgcggtccg atgtgggccg ggtgcccgag
     2281 cccggtggtg cgtcgatgcg ccgcccccct gctcgccccg cgacgatccg cagggcgccc
     2341 ttgctggggg ccgggcgcgc ggaccctgcc gagcggcgag ggcggggcgt cggcttgcga
     2401 ccgctcacgg cacctcggcc acgaggcggg cgctctcgtc ccacagccag cgggcttcct
     2461 cgcggttgcg ggcgtggcgc gatgcgcgcc accgccgccc gtgcgaccag taggcgcccg
     2521 agcgggcggt gatccggggc ttcgaggagc tggcgagcag cacctgggtc ttggccccgg
     2581 ccttgggcga gcggaggacg aagcggccga acgtggtgat ccaccagctg agcgcgcccg
     2641 tgtcgccgtc gacgccgaag ccgctgtaga tgctgccggg gtgcacggcg tagaccgtca
     2701 ccccgtcggc cgcgagctgc cggctgagct ccagggcgaa ctgggcgttg gccagcttcg
     2761 acaggccgta gttgcggccg cctccgaagc ggacgcggcc ctggaggtcg ccgcggtcga
     2821 gccccttgag tgcccaccgg tgcgccaccg acgacagcac gatcacgcgc gacggcgcgc
     2881 tgcggatcag gcgctggcga agcaggtcgg tgaggaggaa gtgcccgagg tggttggtgc
     2941 cgaacatgag ctcgaacccg tcggccgtct cctggcgggt gtcgaccatc agcccggcgt
     3001 tgttgaccag gacgtcgagc cggtcgaact cggcgaggaa ccagctggcg aactcgcgga
     3061 tcgatgcgaa cgaggccaag tcgagccggc cgagcaccac ccggtcgcta ccggaacggg
     3121 cgagcacctc ggtgcgcgcc tcctcgccct tcgagggggt ccgggccgtc atgacgacgg
     3181 tggcccccat cgcggcgagc gcgaccgcgg tctccttgcc gatgcccgag ttggcccccg
     3241 tcacgatgac gaccttcccg ctgaggtcct cgcgctgctt cgcacccatg gccgccgagt
     3301 ctcgcaccct ggtccccggc cctccccaac ccggtggccc gaggggtacc gtcgcccagg
     3361 tgagcgagcc caccgacccc tcccccggcg acgccgtccg gatcgtccgc atcgaccgtc
     3421 ccgagcggcg caacgcggtc aactcggaga cggccgagcg cctctaccgc gagttccagg
     3481 cgttcgactc ggaccccacg gccaaggtgg cgatcctcac cggcgacacc tcgtcgttct
     3541 gcgccggtgc cgatctcacc gacctgcccc ggctccgcga gagcgggccg atgggcccga
     3601 ccaggatggc cctgtcgaag ccggtcatcg ccgcggtcga gggctggtgc gtcgccggag
     3661 gcgtggagct ggcggcgtgg tgcgacatcc gcatcgccgg cgccggcgcc aagttcggct
     3721 gcctcgagcg ccggtggggt gtgccgctca tcgacggcgg caccatccgc ctcccccgca
     3781 tcgtcgggct cggccgggcg atggacctga tgctcaccgg ccgcggcttc gacgccgccg
     3841 aggccgagcg catcggcttc gtcagccgcc tcgtgccgga gggcaccgcg ctcgatgccg
     3901 ccatcgaagc cgccgaggtg atcgcctcgt cgccgtggcc cgccgtcgtc cacgaccgtc
     3961 tctctgccta cgagagctac ggcctgagct tcgaggaggc cctcgccaac gaggaccgcc
     4021 acgggcgagc cgtcatcttc gaccccgggt tcgccgacgg cgtcgcccag ttccaggcga
     4081 accaggccaa gcgccggacc gaaggcgacc ggtgagcgac caccgcgccg acgacgacca
     4141 cgacgagggg ccgaccgact cctccggtgc accgctggtg ccgcagacga tcgtgtgcgt
     4201 cgactgcggg agcacggccc acctcatgac ccggcccgac gacaccggcc ggttctggcc
     4261 cggtgacctg gtgtcctacc ggtgcgagga ctgcatggac cgctgggacc tgatcgttcc
     4321 cggagacgac cccgaagacg accacggccc cggctggtag cgcccggtgt gccgatcagg
     4381 cggcgcgccg ggggcggggc tggagctcgc caccgcagtt ggggcaggca ccggcgagcg
     4441 gcccgtcgac gcagccggcg cagaacgtgc actcgtagga gcagatcctc gcctggtcgc
     4501 tccccggcgg cagcgccgtc ccgcaccgct cgcagaccgc ccgcatctcc agcatcgaag
     4561 cccgccccga cccgctcagt agccgttctt gtgcagcttg tcgatggcga tctgcaggac
     4621 cacgcgctcg ctctggatcg ggttcgggta gcggtcgaca cccatgtact tctcggccag
     4681 cgcgtcgatg tgggcgcggg cctcgtcgcc accgacggtg ccgacgaccc ggcctcgggc
     4741 ctcgacgtac tggtacgcgt tctcgctgtt gaacacggtc acggtgaccc gcgggtcgcg
     4801 ctcggcgttc ttgaacttct ggcggtggat ctccgtgttg atcaggaggt gctcgccgtc
     4861 gtcgccgatc cacatgatct gcgtctgcgg ccggccgtca gccatgagcg tggtgaacgc
     4921 ggcgaagttc ttgccggcga ggagggaggc gaggtctgcg tcgagggtca tggccgagac
     4981 gctaccggga ccgccgggtg atcggggccc gcaggtcgag cggtgctccg ctgtcgccag
     5041 cggcgtcgga ccgaacgccg ggcgccctcg agggagccgg ctcgtagcct gcagccatgc
     5101 gcttcagctt ctggatctcg acgcagctct cctggaccga gacgctcgat ctcgccagcc
     5161 acgccgaggc cacggggtgg gacgggctgt gggtcgccga ccacttcatg cccaacgacg
     5221 acaccgcgct cgaccagccg atgcacgagg cgctcggcct cctcggcgcc ctggccgccg
     5281 ccgtgccgcg cgtgcggctc gggtcgctcg tcctcggcaa cacctaccgg caccccgccg
     5341 tcgtggccaa gacggcggcc accatcgacc acatctcggg cggccggctc gtcctcggcg
     5401 tcggcgccgg ctggcaggag aacgagcacg cccgctacgg catcccgctc ggcacggtgc
     5461 gcgagcgggt ggactggttc gaggaggcat gccaggtgct ggtcggcctg cgcgacgaag
     5521 cccgcaccac ggtggcgggc gctcgctacc agctcgacga cgcgcccgcc gagcccaagc
     5581 ccaccggtcc cctgcccctg ctcatcggcg ccagcggcca gaagcgcatg gccgcgatcg
     5641 tggccgagta cgccgacgag tggaacacgt ggagcacccc gtcgctctgg cgggagaagc
     5701 gcatcggctt cgaccaggcg ctcgaccagg cgggccggga ccgcgctgac ctgtaccggt
     5761 ccacccaggc actcgtgttc ctgggcgatg acggctacca gaccgccgag gacttcgccg
     5821 ccatccgccc ggccatcggc ggcacgagcg agcagctcgt cgcgaccatc ggccagtggg
     5881 ccg
//
LOCUS       DATMFS010000589         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_300032, whole genome shotgun sequence.
ACCESSION   DATMFS010000589 DATMFS010000000
VERSION     DATMFS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300032"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VNQ33_11270"
     CDS             complement(<1..717)
                     /locus_tag="VNQ33_11270"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98731.1"
                     /translation="MTAAPAVSIRGVSRTFGEREVLRGLDLDLRPGEFVALLGRSGSG
                     KSTLLRALAGLDPAVLGDIIVPAARSVVFQDPRLLPWASILDNVVLGLDGTRRGSTAA
                     GREALDEVGLGGHEGDWPKTLSGGEAQRASLARALVRSPKLLLLDEPFGALDALTRIR
                     MHALVQQLHARHQPAVLLVTHDVDEAVVLADRVLVLTDGRFSLDVPVDAASPRLRSDP
                     EIIALRSRLLAELGVDEAAEG"
     gene            complement(714..1607)
                     /locus_tag="VNQ33_11275"
     CDS             complement(714..1607)
                     /locus_tag="VNQ33_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98732.1"
                     /translation="MTHAEYAVAARPVAVEPAPRADDPPVPGPDLVALPTGQAPAADQ
                     RRLGIPHGIERLLGVVVVLSGWALATGVGWISTKTLPRPGTVAVVAVDLWNDGTLQAA
                     LWASIQRVGWGLAIGVPIGTALALVVGLSRLGDDLIDTNISALRFVPIIALQPLLVVW
                     LGVGETVKITMIVLGVAFPIYINTTTAIKSLDPGYRELADVVGLSKGQLIGRVVLPGA
                     LPGFLVGLRMSVAISWLLLVFAETINATSGLGKMMSDASLFAKTDVLILGLIIYAVLG
                     LATDAGVRLLERRLLSWQPGR"
     gene            complement(1604..>2089)
                     /locus_tag="VNQ33_11280"
     CDS             complement(1604..>2089)
                     /locus_tag="VNQ33_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98733.1"
                     /translation="ATLAGGSADAGLSTEPLISLYLQDHPEATLAIDAGNITDRASFL
                     IASKKTLEDDAKTAALADFTTRLVKAFAYLNDHKGDLPEAVFVKQYGLTPERAKELVD
                     SGNGSTEFFPLPGDILEPQQKVADLFTAAGQIPDEIDVTREFDSRFNDVVAAAQKEEG
                     P"
BASE COUNT          298 a          685 c          829 g          277 t
ORIGIN      
        1 gccttcggcc gcttcgtcca cgccgagctc ggcgagcagg cgcgatcgga gggcgatgat
       61 ctcgggatcg ctgcgcaggc gcgggctggc ggcgtcgacg gggacgtcga gcgagaaccg
      121 gccgtcggtg aggacgagga cgcggtcggc caagaccacg gcctcgtcga cgtcgtgggt
      181 cacgagcagc acggcgggct ggtggcgggc gtggagctgc tggacgaggg cgtgcatgcg
      241 gatccgggtg agcgcgtcga gcgccccgaa cggctcgtcg aggagcagga gcttgggcga
      301 gcgcaccagg gcccgggcga gcgacgcccg ctgggcctcg ccgcccgaga gggtcttggg
      361 ccagtcgccc tcgtgaccgc cgaggccgac ctcgtcgagc gcctcgcggc cggcggcggt
      421 gctgccgcgg cgggtgccgt cgagcccgag gaccacgttg tcgaggatcg acgcccacgg
      481 cagcagccgc gggtcctgga acaccaccga ccgggcggcc ggcacgatga tgtcgcccag
      541 gactgcgggg tcgaggccgg cgagggcccg cagcagcgtg ctcttgcccg atccgctgcg
      601 ccccagcagg gcgacgaact cgccgggccg gaggtcgagg tccagcccgc gcaggacctc
      661 gcgctcgccg aaggtgcggg agacgccgcg gatcgagacg gccggggcgg cggtcaccgg
      721 cccggctgcc aggagaggag gcggcgctcg aggagccgga cgccggcgtc ggtcgcgagc
      781 ccgaggaccg cgtagatgat cagcccgagg atgaggacgt cggtcttggc gaacagcgag
      841 gcgtcggaca tcatcttgcc gaggccgctg gtggcgttga tggtctcggc gaagaccagc
      901 aggagccacg agatggccac ggacatgcgc agcccgacga ggaagccggg gagcgctccg
      961 ggcagcacga cccggccgat gagctggccc ttgctgagcc cgaccacatc ggcgagctcg
     1021 cggtagcccg ggtcgaggct cttgatggcc gtggtggtgt tgatgtagat cgggaacgcc
     1081 acgcccagca cgatcatggt gatcttcacc gtctcgccga cgccgagcca caccaccagc
     1141 agcggctgga gcgcgatgat cgggacgaac cgcaacgccg agatgttggt gtcgatcagg
     1201 tcgtcgccga gccgggacaa cccgacgacc agcgcgaggg cggtgccgat ggggacgccg
     1261 atcgccaggc cccagcccac ccgctggatg gatgcccaga gcgccgcctg gagggtgccg
     1321 tcgttccaga ggtcgacggc gaccacggcg acggtcccgg gtctgggcag cgtcttggtg
     1381 ctgatccagc cgacaccggt ggccagcgcc cagccgctca ggaccacgac gacgcccagc
     1441 agccgctcga tcccgtgggg gatgcccagc ctccgctggt ccgccgccgg cgcctgtccg
     1501 gtcggaaggg cgacgagatc gggcccgggg acgggcgggt cgtcggcgcg gggcgcgggc
     1561 tcgaccgcga ccggtcgggc cgccaccgcg tactcggcgt gggtcacggt ccctcctcct
     1621 tctgggcggc ggccacgacg tcgttgaacc ggctgtcgaa ctcgcgggtc acgtcgatct
     1681 cgtcggggat ctgcccggcc gcggtgaaca ggtcggcgac cttctgctgg ggctcgagga
     1741 tgtcgccggg gagcgggaag aactcggtgg agccgttgcc gctgtcgacc agctccttgg
     1801 cccgctctgg cgtgagcccg tactgcttca cgaacacggc ctcgggcagg tcgcccttgt
     1861 ggtcgttgag gtaggcgaag gccttgacga ggcgggtggt gaagtcggcg agcgccgcgg
     1921 tcttggcgtc gtcttcgagc gtcttcttcg atgcgatgag gaacgaggcc cggtcggtga
     1981 tgttgccggc gtcgatcgcc aaggtggcct ccgggtggtc ctggaggtag aggctgatga
     2041 gcggctcggt ggagagcccg gcgtcggccg acccgccggc gagggtggc
//
LOCUS       DATMFS010000590         5422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_346513, whole genome shotgun sequence.
ACCESSION   DATMFS010000590 DATMFS010000000
VERSION     DATMFS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346513"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VNQ33_11285"
     CDS             <1..1893
                     /locus_tag="VNQ33_11285"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWJ98734.1"
                     /translation="HHVTPVERTVTWFQTGDTTIKAGTLIDGLSALMLVVVTFISLLV
                     HVFSTDYVGGDRRETHYFAFLSLFTAAMLFYVISSNTLQMVVGWELVGLCSFALIGHW
                     WEEKPNSDAALKAFLTNRVGDIGLLIGVTILYFAAGRTFDVLSLNTAANLPQGSPGSI
                     SHGLLLAGSLCLITAVMSKSGQFVLHTWLPDAMAGPTPVSALIHAATMVVAGIYLVAR
                     NYGVFFNGLSIGTANVNALAVVGCVTLLFGAALAFVQTDIKKVLAYSTVSQLGYMVAA
                     LGVGAWTAAIFHLFTHAVFKACLFLGSGSLAHHIHSFDMKKDMGGMRKRMPITHATFV
                     IATLALMGFPLITAGFWSKDEILAGAHMLGGDGGYKFAVVMGLLGAVCTCAYMTRAYY
                     YVFMGEPRGKSAEHELHENGPRITVPLMILAFGSIVVGWANLPEKVFGIGVPDSLSLR
                     FEHFIEPTGLYFPSADNNFSHPEFVPWIALVSALAIVIGVGSAYLWYWKGKGPHGITE
                     RSKVARGGYQVLENKYYLDWLYTDVITGFVKGPFAKATNWFNQNVLDGVVNSVGEGAR
                     KAGGFVYHRIDQGVVDNVVKGSGLAAEGSGSVLRKSQNGKVQWYAAYLFLGATILAAI
                     FVVVAS"
     gene            1913..3460
                     /locus_tag="VNQ33_11290"
     CDS             1913..3460
                     /locus_tag="VNQ33_11290"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWJ98735.1"
                     /translation="MKDLLNGWGITAAVFLPMVGALVMLAIPKSEETLHKVVALVTSL
                     AVFAVGVALLWDFDYDHTAVLQYAQDKSWIELINARYLVGIDGISLPLIALTMLVVPL
                     VIVYSWDHFPEPHNPKAFLLLILVLETGMIGTFVAQDLLLFFVFFELVLLPMYFMIGV
                     WGGEQRKYASLKFFLYTAFGSALMIVSILAVYFLAEDKAGNALHTFAIPELAQWGGYG
                     LAKSTALLIFAGFFMGFGIKVPMFPFHTWLPDAHTQAPTQGSVILAAVLLKLGTYGFV
                     RIAVPILPVGAQSWAPVIGLLAVIGIIYGALGCLAQTDMKRLIAFSSVAHMGYVMLGI
                     ATLTPFGTNAAIFGMVAHGLITGMLFFIAGSVKDRFHTLEIKRLGGLLLQAPRMGWLL
                     GFSVMASLGLPGLAGFWGEFPAILSAWEPGADLNQNLFRAFAVVAALGTVFAAGYLLW
                     LLQRVAFGTPTEEFEGEHIHDMHLTDYIAWVPMVVLIVVLGIFPGLIFKVTNQAVVHS
                     LMHLPGS"
     gene            3460..4986
                     /locus_tag="VNQ33_11295"
     CDS             3460..4986
                     /locus_tag="VNQ33_11295"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWJ98736.1"
                     /translation="MVLATLLAAASPVFHRPEFDWHALAPELVLVGTIALCLLVDVFT
                     TEQSRWTASSIAGIGMLAALVPVFTLAYDNTDRVLFGGAFVVDDFSLVLSGLFLVAGY
                     LTILLSTQYIAEGDYAEGEYYLMLLSATFGMVVMASARDLIGIFIALEMLSIPAYLMA
                     GWRKRTLTGNEAGMKYYLMGVFASGVMLYGMSLIFGVTGSTILKDIGSQVNASVMDEP
                     IAILGIIFVLIGFAFKVSAVPFQNWAPDTYEGAPTPVTAFLSVASKTAGFVAILSLVY
                     VGFAGQADIIRPFIFILAVLTMTVGNVIALRQTNVVRLFAYSSVAQAGFILAPLAVFG
                     ESPRSSRDVLIAVVTYVIIYTVVNLGAFAVIIAVSRRTGTGDIASWGGLFTYAPALAV
                     SMAAFLFALGGIPIAAGWYAKFQAFTAVVSGDSNLGYIMGVVMAVNSVIALGYYLSLM
                     RVMFMDDVPDGDTTPIRVPVTLGVVVGAAVIFTLAVGIFPGLVSDLAEGATLAFLPGS
                     "
     gene            4997..>5422
                     /locus_tag="VNQ33_11300"
     CDS             4997..>5422
                     /locus_tag="VNQ33_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98737.1"
                     /translation="MGELEQLLVAHIAATGPIGFDEYQAHALYAPRLGFYQRGGGAGR
                     RRDFVTSPEIGPLFGAVLARALDTWWHELGRPERFHLVDAGAGPGGLTRSVLAASPAC
                     SGALACTLVEVGEAQWATHPDGTDSRVDLPAPRELDGPVV"
BASE COUNT          745 a         1980 c         1667 g         1030 t
ORIGIN      
        1 caccacgtca cgccggtgga gcgcaccgtc acctggttcc agaccggcga caccaccatc
       61 aaggccggca ccctcatcga cgggctcagc gccttgatgc tcgtggtcgt cacgttcatc
      121 tcgctgctcg tgcacgtctt ctccaccgac tacgtcggcg gcgaccggcg tgagacccac
      181 tacttcgcct tcttgagcct gttcaccgcg gcgatgctct tctacgtcat ctccagcaac
      241 acgttgcaga tggtcgtggg ctgggagctc gtcggcctct gctcgttcgc cctcatcggt
      301 cactggtggg aggagaagcc gaactccgac gccgccctca aggccttcct caccaaccgc
      361 gtgggcgaca tcgggctgct catcggcgtc acgatcctgt acttcgccgc cggccgcacg
      421 ttcgacgtgc tcagcctcaa caccgcggcc aacctcccgc aaggatcgcc agggagcatc
      481 tcccacggcc tgctcttggc cggatcgctc tgcctcatca ccgccgtgat gtcgaagtcg
      541 ggccagttcg tcctgcacac ctggctgccc gacgccatgg ccggtcccac cccggtctcg
      601 gcgctcatcc acgccgccac catggtcgtc gccggcatct acctcgtggc ccgcaactac
      661 ggcgtgttct tcaacggctt gtccatcggc accgccaacg tgaacgccct cgccgtggtc
      721 ggctgcgtca ccctcctctt cggcgccgcg ctggccttcg tccagaccga catcaagaag
      781 gtgctggcgt actcgacggt gtcgcagctc ggctacatgg tcgccgctct cggcgtcggc
      841 gcttggaccg cggccatctt ccacctcttc acccatgccg tcttcaaggc ctgcctgttc
      901 ctcgggtcgg gctcgctggc ccaccacatc cactccttcg acatgaagaa ggacatgggc
      961 ggcatgcgca agcggatgcc gatcacccac gccaccttcg tgatcgccac cctcgccctg
     1021 atgggcttcc cgctcatcac cgccggcttc tggtccaaag acgagatcct cgccggcgcc
     1081 cacatgctgg gcggcgacgg gggctacaag ttcgccgtgg tcatgggcct cctcggcgcc
     1141 gtgtgcacct gcgcctacat gacccgggcc tactactacg tcttcatggg cgagccccgc
     1201 gggaagtcgg ccgagcacga gctgcacgag aacgggcccc gcatcaccgt gccgctcatg
     1261 atcttggcct tcggctcgat cgtggtgggc tgggccaacc tccccgagaa ggtgttcggc
     1321 atcggcgtac ccgattcgct gtcgctgcgc ttcgagcact tcatcgagcc gaccgggctc
     1381 tacttcccga gcgccgacaa caacttcagc caccccgagt tcgtgccgtg gatcgccctg
     1441 gtctcggccc tggccatcgt gatcggcgtc ggcagcgcct acctctggta ctggaagggc
     1501 aagggccccc acggcatcac cgagcgcagc aaggtcgccc gcggcgggta ccaggtgctg
     1561 gagaacaagt actacctgga ctggctctac accgatgtga tcaccggctt cgtcaagggc
     1621 ccgttcgcca aggccacgaa ctggttcaac cagaacgtcc tcgacggcgt cgtgaactcg
     1681 gtgggcgaag gcgcccgcaa ggccggcggc ttcgtctacc accgcatcga ccaaggcgtg
     1741 gtcgacaacg tggtgaaggg ctcgggcctc gccgccgaag gctctggatc ggtcctgcgc
     1801 aagtcgcaga acggcaaggt ccagtggtac gccgcatacc tgttccttgg cgccaccatc
     1861 ctggccgcca tcttcgttgt agtcgctagt tgaggacgag ggagtcgaaa ccgtgaagga
     1921 cctgctcaac ggctggggta tcaccgccgc cgtgttcctg cccatggtgg gagcgctggt
     1981 gatgctggcg atccccaagt cagaggagac cctgcacaag gtggtggccc tggtcaccag
     2041 cctggccgtg ttcgcggtcg gagtcgccct gctgtgggac ttcgactacg accacaccgc
     2101 cgtgctgcag tacgcgcagg acaagtcgtg gatcgagctc atcaacgctc ggtacctcgt
     2161 gggcatcgac ggcatctcgc tgccgctgat cgccctgacc atgctggtcg tgcccctcgt
     2221 catcgtctac tcgtgggacc acttccccga gccgcacaac ccgaaggcgt tcctgctgct
     2281 gatcctggtc ctcgagaccg gcatgatcgg cacgttcgtg gcccaggacc tgctcttgtt
     2341 cttcgtcttc ttcgagctgg tgctgctccc gatgtacttc atgatcgggg tctggggcgg
     2401 tgagcagcgc aagtacgcgt cgctgaagtt cttcctctac acggcgttcg gctcggcgct
     2461 catgatcgtg tcgatcctcg ccgtctactt cctggccgag gacaaggcgg gcaacgccct
     2521 ccacacgttc gcgatcccgg agctggcgca atggggcggg tacgggctgg ccaagagcac
     2581 ggcgctgctg atcttcgccg gcttcttcat gggcttcggc atcaaggtcc cgatgttccc
     2641 gttccacacc tggctgcccg acgcccacac ccaggcgccc acccagggct cggtcatcct
     2701 cgccgcggtg ctgttgaagc tcggcaccta cggcttcgtc cgcatcgcgg tcccgatcct
     2761 gcccgtgggc gcccagtcgt gggctccggt catcggcctg ctggcggtca tcggcatcat
     2821 ctacggcgcc ctcggctgtc tggcccagac cgacatgaag cgcctgatcg cgttctcgtc
     2881 ggtcgcccac atgggctacg tgatgctcgg catcgccacg ctcacgccgt tcggcaccaa
     2941 cgccgccatc ttcggcatgg tcgcccacgg cctcatcacc ggcatgctct tcttcatcgc
     3001 cggatcggtg aaggaccggt tccacaccct cgagatcaag cgcctgggcg gcctgctcct
     3061 ccaggccccc cgcatgggct ggctgctcgg cttctcggtc atggcctcgc tcggcctccc
     3121 cggcctcgcc gggttctggg gcgagttccc ggccatcctc tcggcgtggg agccgggtgc
     3181 ggacctcaac cagaacctgt tccgggcgtt cgccgtcgtc gccgccctgg gcacggtgtt
     3241 cgccgccggc tacctcctct ggctgctcca gcgggtggcc ttcggcacgc ccaccgagga
     3301 gttcgaaggt gagcacatcc acgacatgca cctcaccgac tacatcgcct gggtcccgat
     3361 ggtcgtgctg atcgtcgtgc tcggcatctt cccgggcctg atcttcaagg tcaccaacca
     3421 ggccgtcgtc cacagcctca tgcacctgcc cgggagctga tggtgctcgc aaccctcctc
     3481 gccgccgcgt cgccggtctt ccaccggccg gagttcgact ggcacgcgct cgcgcccgag
     3541 ctcgtcttgg tcgggaccat cgcgctgtgc ctgctggtcg acgtgttcac caccgagcag
     3601 agccggtgga cggcctcgtc catcgccggc atcggcatgc tggcggccct cgtcccggtc
     3661 ttcacgctgg cctacgacaa caccgaccgg gtgctgttcg gcggggcgtt cgtggtcgac
     3721 gacttctcgc tggtgctcag cgggctcttc ctcgtcgccg gctacctcac gatcctgctg
     3781 tccacgcagt acatcgcgga gggggactac gccgagggcg agtactacct gatgctgctg
     3841 tcggccacct tcggcatggt ggtcatggcg tcggcccgcg acctgatcgg gatcttcatc
     3901 gccctcgaga tgctctccat cccggcgtac ctcatggcgg gctggcgcaa gcgcacgctc
     3961 accggcaacg aggccggcat gaagtactac ctgatgggcg tgttcgcctc gggcgtcatg
     4021 ctctacggca tgtcgctgat cttcggcgtc accggctcga cgatcctgaa ggacatcggc
     4081 tcgcaggtga acgccagcgt gatggacgag cccatcgcca tcctcggcat catcttcgtg
     4141 ctcatcgggt tcgccttcaa ggtctcggcc gttccgttcc agaactgggc gccggacacc
     4201 tacgagggtg cgccgacccc ggtcaccgcc ttcttgtcgg tggcgtccaa gacggctggc
     4261 ttcgtagcca tcctctcgct cgtctacgtc gggttcgccg gtcaggccga catcatccgg
     4321 ccgttcatct tcatcctcgc cgtgctcacc atgacggtgg gcaacgtgat cgccctccgc
     4381 cagaccaacg tggtgcgcct gttcgcctac tcgtcggtcg cccaggccgg gttcatcctc
     4441 gccccgctcg cggtcttcgg cgagagcccc cgctcctccc gcgacgtgct catcgccgtg
     4501 gtgacctacg tgatcatcta cacggtggtg aacctcggcg ccttcgccgt gatcatcgcc
     4561 gtcagccgcc gcaccggcac cggtgacatc gcctcctggg gcggcttgtt cacctacgcc
     4621 ccggcgctgg ccgtgtcgat ggccgcgttc ttgttcgccc tcggcggcat cccgatcgcg
     4681 gctggctggt acgccaagtt ccaggcgttc accgcggtgg tctccggcga cagcaacctc
     4741 ggctacatca tgggcgtcgt catggccgtg aactcggtca tcgcgctcgg gtactacctg
     4801 tcgctcatgc gggtcatgtt catggacgac gtgcccgacg gcgataccac gccgatccgg
     4861 gtgccggtca cgctcggcgt ggtcgtaggt gcggccgtga tcttcaccct ggcggtcggc
     4921 atcttcccgg gtctggtcag cgacctggcc gaaggagcca ccctcgcctt cttgcccggg
     4981 agctgagccc ggcgccatgg gggagctcga gcagctgctc gtcgcccaca tcgccgccac
     5041 cggccccatc ggcttcgacg agtaccaggc gcatgccctc tacgcgcccc gcctcggctt
     5101 ctaccagcgc ggcgggggag ccgggcggcg gcgcgacttc gtgacctcgc ccgagatcgg
     5161 cccgctcttc ggggcggtcc tggcccgggc gctcgacacg tggtggcacg agctcggccg
     5221 acccgagcgg ttccacctgg tggacgccgg cgccggtccc ggcgggctga cccgctcggt
     5281 cctggctgct tcgcccgcgt gcagcggtgc gctggcgtgc acgctcgtcg aggtgggcga
     5341 ggcccagtgg gcgacgcacc ccgacggcac cgacagccgg gtcgacctcc cggcgccccg
     5401 cgagctcgac gggccggtgg tg
//
LOCUS       DATMFS010000591         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_136676, whole genome shotgun sequence.
ACCESSION   DATMFS010000591 DATMFS010000000
VERSION     DATMFS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136676"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VNQ33_11305"
     CDS             <1..1392
                     /locus_tag="VNQ33_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWJ98738.1"
                     /translation="GGSDSGFLFIETEEQTSVCVDLIELAPEGDASALLTREDICARV
                     HDRIHLLPSWRWRLEPVPLRIHHPVWVDDPEFVVEDHIRHRVLPAPGGPEEVNALLAE
                     LEPLRLDLRHPLWQIVLVDGLGGPGSTRQALVLRVHHTVADGAAILHTFDLLFGDAAG
                     ELPRPERSVVPPLPTKQALLADATREQLRNWRQIPRMFKDTKAIFEKVEARRAEAVAV
                     VPRPMHDAPGTILNQPGPAIRTYARTRVPIADLQRVRKTTGATLNDVALGMVAGSLRA
                     YLLARDALPAKGLVANVPVSGDPPGTPPRQWGNRFANFFALLHTDVDDPRERLAAISA
                     STAEAKLQLQIQGHDTLTEWLDRMPPLIGRPAARAMVNRSAKDPDTADFNALVSNVRI
                     TARDWRIGDQRVAHVYLSGPIGDDTGLNITVVGYDDDLHVTVVGNPVTMERPDEVADG
                     MRLALEELIACTA"
     gene            1380..>2409
                     /locus_tag="VNQ33_11310"
     CDS             1380..>2409
                     /locus_tag="VNQ33_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWJ98739.1"
                     /translation="MHRLSGEDAGFLSMEQPEQAMNTIAVGSLRAPGGGPTGVTIEDV
                     RTHIAGRLDQLPSFRWRIVAVPFGLHHPMAVRDPDFELDYHLREATLADGQSLDDLFA
                     HLAEQRLDRRHPLWQLTLVHGLPDGEQALILKYHHCLADGVAAFTTFSRVFSDAPTEP
                     IPDVELPWKPEKVPSKLLLVWLAFLDHLAALLSIPGIALRAKRGFDAVKARRSKTAVA
                     VPEFSGAAPNSALNDAFTPERAYARAVLPLAAVKEAKDAAGVTLNDVVLATVAGGLRG
                     YLADRDELPSLPLLASVPVSYEAADAPVRQAGNRFWSFTTSLATDVADPAERLAAISA
                     TAAEAKEQL"
BASE COUNT          338 a          966 c          788 g          317 t
ORIGIN      
        1 gggggaagcg attcggggtt cctgttcatc gagacggagg agcagacctc cgtctgcgtc
       61 gacctgatcg aactggctcc cgaaggcgac gccagcgccc tgttgaccag ggaggacatc
      121 tgcgcccggg tccacgaccg catccacctg ctgccgtcgt ggcgctggcg gctcgagccg
      181 gtgccgctgc gcatccacca ccccgtctgg gtcgacgacc ccgagttcgt ggtcgaggac
      241 cacatccgcc accgggtcct cccggcgccg gggggtcccg aggaggtcaa cgcgctcctg
      301 gccgagctgg agccgctgcg cctggacctg cgccaccccc tgtggcagat cgtgctggtc
      361 gacgggctgg gcggccccgg cagcacccgc caggcgctcg tgctgcgggt ccaccacacc
      421 gtcgccgacg gcgccgccat cttgcacacc ttcgacctgc tgttcggcga cgccgcgggc
      481 gagctgcccc ggcccgagcg ctccgtcgtc ccgcccctgc ccaccaagca ggcgctgctg
      541 gcggatgcga cccgggagca gctccgcaac tggcgtcaga tcccgcggat gttcaaggac
      601 accaaggcga tcttcgagaa ggtcgaagcg cggcgcgccg aggccgtcgc cgtcgtgccc
      661 cggcccatgc acgacgcgcc cggcacgatc ctgaaccagc ccggcccggc gatccgcacc
      721 tacgcccgca cgagggtccc gatcgccgac ctccagcggg tccgcaagac caccggcgcc
      781 acgctcaacg acgtcgcgct cggcatggtg gccggttccc tgcgggccta cctgctcgct
      841 cgagacgcgc tgcccgcgaa gggcctcgtg gccaacgtcc ccgtctcggg cgatccgccg
      901 ggcacgcccc ctcggcagtg ggggaaccgg ttcgccaact tcttcgccct gctgcacacc
      961 gatgtcgacg acccccgcga gcgcctggcg gccatcagcg ccagcacggc cgaggccaag
     1021 ctccagctgc agatccaggg ccacgacacg ctcaccgagt ggctcgaccg catgccgccg
     1081 ctgatcgggc gcccggccgc ccgggccatg gtgaaccgga gcgccaagga cccggacacc
     1141 gccgacttca acgccctggt ctccaacgtc cggatcaccg cccgcgactg gcgcatcggt
     1201 gaccagcggg tggcgcacgt gtacctgtcc gggcccatcg gcgacgacac cgggctgaac
     1261 atcaccgtgg tcggctacga cgacgacctc cacgtcacgg tcgtcggcaa cccggtgacc
     1321 atggagcgtc ccgacgaggt cgccgacggg atgcggctcg ccctcgagga gctgatcgca
     1381 tgcaccgctt gagcggcgag gacgccggct tcctctcgat ggagcagccc gagcaggcga
     1441 tgaacaccat cgccgtcggc tcgctccggg cgcccggcgg cggccccacc ggcgtgacca
     1501 tcgaggatgt ccgcacccac atcgccgggc gcctcgacca gctcccgtcg ttccgctggc
     1561 gcatcgtcgc ggtgccgttc ggcctgcacc acccgatggc cgtgcgggac ccggacttcg
     1621 agctcgacta ccacctccgc gaggccacgc tggccgacgg gcagtcgctc gacgacctgt
     1681 tcgcccacct ggccgagcag cgcctcgacc gccgccaccc gctctggcag ctcacgctcg
     1741 tgcacggcct ccccgacggc gagcaggcgc tgatcctcaa gtaccaccac tgcctggccg
     1801 acggcgtggc cgccttcacc accttctccc gcgtgttctc cgacgcgccc accgagccca
     1861 tccccgacgt cgagctgccg tggaagccgg agaaggtgcc gtcgaagctg ctgctggtct
     1921 ggctggcgtt cctcgaccac ctcgccgccc tgctgtcgat cccgggtatc gcgctgcggg
     1981 ccaagcgcgg gttcgacgcc gtgaaggccc gccggtccaa gacggccgtg gccgtgccgg
     2041 agttcagcgg cgccgcaccc aacagcgcgc tcaacgacgc cttcaccccc gagcgcgcct
     2101 acgcccgggc cgtcctcccc ctcgcggcgg tgaaggaggc gaaggatgcg gccggcgtca
     2161 cgctcaacga cgtcgtgctc gccaccgtgg ccggcggcct gcgcggctac ctcgcggacc
     2221 gcgacgagct gccgtcgctc cccctgctcg ccagcgtccc ggtgtcctac gaggcggccg
     2281 acgccccggt gcgccaggcc ggcaaccggt tctggagctt caccacctcg ctcgccaccg
     2341 acgtcgccga cccggccgag cgcctggccg ccatcagcgc cacggcggcc gaggcgaagg
     2401 agcagctca
//
LOCUS       DATMFS010000592         5395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_346636, whole genome shotgun sequence.
ACCESSION   DATMFS010000592 DATMFS010000000
VERSION     DATMFS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346636"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2351
                     /locus_tag="VNQ33_11315"
     CDS             <1..2351
                     /locus_tag="VNQ33_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ98740.1"
                     /translation="IPVDESTLPAVIASYQLFSELDLGHFDRIITTKYPAWMVEHDHK
                     TILMMHVLRGVYDTYQYHHQPLTVPNPSDHVFAMLHLMRTNPGNRGALAEFFERWANA
                     LAALGPDHPDFVFPGPFARLAIRWLDGIGMAPGAVQQYLSQSKTVARREGYFPSTVTP
                     RIVNLPGHLPPAPVNDEHGTYLFTASRLDGPKRLDLLIDAMRYVPGDIALRIAGTGAL
                     RAQLEAQAAGDPRIEFLGFTRNDLLPELYANALAVPFIPADEDLGLITLEAFSQGTAV
                     VTCTDSGGPTEFVKDGSTGLVAEPNAASIGAKLATLVADPGLAHRLGLAGRERGERIT
                     WEGVVEALLGDERFTPEELAARTAASDDEVATSRRSRRRAVVLTTFPIGDPGHGGQLR
                     ARNLYGALATVRPVEVVALVDFGNLPAVQTLAPNLTQVVVPRSAAHHLKGQELSIEAN
                     VPVTDVVAGSHIGLTPAYLSAVAEAADGASAVILAEPYLLPVVEQLELDIPVVYDAYN
                     VEASLKAGVFPDSPVGAELLAQVVDVERRAVQRSARTTTCSEADAAALGAAAGVGADR
                     FSVIANGTTIPDHVVAPEDRAALGARWRRRYWTAGSIGAEPEHLAVFFGSWHPPNLDA
                     AELLIEIAPLLPTTLILSVGFHGQAFRDRILPPNVVFPGTVPGRAKNLLLQAADVALN
                     PMRLGSGTNLKLLEYLAVGTPVVSTPFGARGIDVVDGEHLRFADPDRFAAVTAEVLDD
                     PAGARTRAEAARARVSERYSWERLGSALADVISDIVPAEARR"
     gene            2364..3326
                     /locus_tag="VNQ33_11320"
     CDS             2364..3326
                     /locus_tag="VNQ33_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98741.1"
                     /translation="MSTSIDPPPAPHEPMIPDRVSVSPALEVSKRPNPFKRVGHLWQY
                     RELIGNLTRKELKVKYKNSILGFIWSLLNPLMYLVVFSLVFNIISATPVPYYGIFFLS
                     GLLAWNLFNTALGQGTSAITSNAQLVTKVWFPREALVLASVFSALVHFFLQSIVLLFA
                     LLAFQRSPDYSFLPIFLFALVVLMLFATALAVGLSAVNVYLRDTQHLLEVALLGWFWL
                     SAVAYPFGLVQTKLEDKWGAGAEWLAGLNPVLTVVITFQRTLYNPDYSGTSTSAVNEA
                     NSLPYHPWTWYMTLLGGALAASLVLLFGSLWLFSKLEDNFAEEI"
     gene            3326..4654
                     /locus_tag="VNQ33_11325"
     CDS             3326..4654
                     /locus_tag="VNQ33_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98742.1"
                     /translation="MGELAIEVANVTKSFKVYQEGGQSAKERLIRAGRNPHIVFKALD
                     DVSFEVKQGETFALLGHNGSGKSTMLKCIAGTLRPSTGKVVTRGRLAALLELGAGFHP
                     DLTGRENIYLNGSILGFSKTQIDVIFDDIVEFAELSDFIDQQVKHYSSGMYARLGFSV
                     AINVEPEILLVDEVLSVGDESFQRKCIDRVRQLQGDGRTILLVSHAAELVRQIADRAA
                     VLDHGHLVDIAEPGEAIRTLRETLARRGIALLAEDEDVTEPPPAPKYADPPSLTVPVV
                     KVDKELVITKVVAEYPDPEAKFLLPNQPMRLRIDYFAPQRVTDICMAFEFQDLHGNVI
                     FGDDTQTMEQPIHACDGVGAVCFDLPRVPLLDGAYLISVTATTRNGGQVYDRRDLQDR
                     FEVMQPGRQRGTVALEPKVVHFFHEPSVGEDAPEAEGSDPAAGPGVATTS"
     gene            4651..>5395
                     /locus_tag="VNQ33_11330"
     CDS             4651..>5395
                     /locus_tag="VNQ33_11330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ98743.1"
                     /translation="MSTTEAAVEAPPVGGAGFAYRPGLTHLRALSVVLVLLFHAGVVK
                     SGYIGVDVFFVLSGFLISNILWEKLSGSDPARQVLGSFYARRCLRILPLSLLVLAVTA
                     VASHLFFETLIDNWTSAGRAALVWSENWFLIRESNDYFAPTGTNPFQQYWSLGVEEQF
                     YVVLPLLMMFLLFVLRGFNRRARLIGLTVAAGVGVAAALASSHLLDMSESETYYSSFV
                     RSYQLLAGVLIMAAVRVFKLRDDRRWLFAI"
BASE COUNT          767 a         2049 c         1710 g          869 t
ORIGIN      
        1 agatcccggt cgacgagtcc acgctcccgg ccgtgatcgc cagctaccag ctgttctcgg
       61 aactggacct gggccacttc gaccggatca tcaccacgaa gtaccccgcg tggatggtcg
      121 agcacgacca caagacgatc ctgatgatgc acgtgctgcg cggcgtgtac gacacctacc
      181 agtaccacca ccagccgctc acggtcccga acccctcgga ccacgtgttc gccatgttgc
      241 acctcatgcg gacgaacccc ggcaaccggg gcgcgctcgc ggagttcttc gaacggtggg
      301 ccaacgccct cgccgccctc ggccccgacc accccgactt cgtgttcccc gggccgttcg
      361 cccggctcgc catccgctgg ctcgacggca tcgggatggc gcccggtgcc gtgcagcagt
      421 acctgtcgca atccaagacg gtggcgcgcc gcgaggggta cttcccgtcg accgtcacgc
      481 cccggatcgt caacctgccc ggccacctcc caccggcgcc ggtcaacgac gagcacggca
      541 cctacctctt cacggcctcg cggctcgacg ggcccaagcg gctcgacctg ctgatcgacg
      601 cgatgaggta cgtgcccggc gacatcgcgc tgcgcatcgc cggcaccggc gccctccggg
      661 cgcagctcga ggcgcaggcg gcgggcgacc cgcgcatcga gttcttgggc ttcacccgca
      721 acgacctgct gcccgagctg tacgccaacg ccctggccgt gccgttcatc cccgccgacg
      781 aggacctcgg gctcatcacc ctggaggcgt tctcccaggg caccgccgtg gtcacctgca
      841 ccgactcggg cggacccacc gagttcgtca aggacgggtc caccggcctg gtggccgagc
      901 cgaacgcagc ctcgatcggc gccaagctcg ccacgctcgt cgccgacccc gggctggcgc
      961 accgtctggg cctcgccggc agggaacggg gcgagcgcat cacctgggaa ggcgtggtcg
     1021 aggcgctcct gggcgacgag cgcttcaccc ccgaggagct cgcggcgcgc accgccgcaa
     1081 gcgacgatga ggtcgccacg tcgcgacgca gccggcggcg cgccgtcgtg ctcaccacct
     1141 tccccatcgg cgatcccggc cacggcggcc agctgcgcgc ccgcaacctc tacggtgccc
     1201 tcgccacggt ccggccggtc gaggtggtgg cgctggtcga cttcggcaac ctcccggccg
     1261 tccagacgct cgccccgaac ctgacccagg tggtcgtccc ccgctcggcc gcgcaccacc
     1321 tgaagggcca ggagctcagc atcgaggcga acgtccccgt gaccgacgtc gtcgccggga
     1381 gccacatcgg cctcaccccg gcctacctgt cggccgtcgc cgaggcggcc gatggtgcca
     1441 gcgccgtcat cctcgccgag ccgtacctcc ttcccgtggt ggagcagctc gagctggaca
     1501 tcccggtcgt ctacgacgcc tacaacgtcg aggcctcgct caaggccggc gtcttcccgg
     1561 actcaccggt cggcgcggag ctgctggccc aggtggtcga cgtcgagcgc cgcgccgtgc
     1621 agcgctcggc gcgcaccacc acgtgctccg aagccgatgc cgcggcgctg ggtgcggcgg
     1681 ccggggtcgg agccgatcgc ttctccgtca tcgcgaacgg gacgacgatc cccgaccacg
     1741 tggtcgcccc cgaggaccgg gccgcgctgg gtgcccggtg gcggcgccgc tactggaccg
     1801 ccggcagcat cggcgccgag cccgagcacc tcgcggtctt cttcgggtcg tggcacccgc
     1861 cgaacctcga cgccgccgag ctgctgatcg agatcgcccc gctgctgccc acgacgttga
     1921 tcctctcggt cgggttccac ggccaggcgt tccgcgaccg gatcctgccg ccgaacgtcg
     1981 tgttccccgg gacggtcccc ggccgcgcca agaacctgct cctccaagcg gccgacgtgg
     2041 ccctcaaccc gatgcggctc ggctcgggca cgaacctcaa gctcctcgag tacctggcgg
     2101 tgggcacgcc cgtcgtgtcg acgccgttcg gcgcacgcgg catcgacgtc gtcgacggcg
     2161 agcacctgcg cttcgccgac cccgaccggt tcgccgccgt gaccgccgag gtcctcgacg
     2221 atccggccgg cgctcgcacc cgggccgagg cggctcgcgc ccgggtcagc gagcgctaca
     2281 gctgggagcg gctcggctcg gccctggccg atgtcatctc cgacatcgtt ccggctgaag
     2341 cccgccggta acctcgtccc gctgtgtcca cctcgatcga tcctcccccc gcgccccacg
     2401 agccgatgat cccggaccgc gtctcggtct cacccgccct cgaggtctcg aagcggccca
     2461 acccgttcaa gcgggtcggg cacctgtggc agtaccgcga gctgatcggc aacctgaccc
     2521 gcaaggaact gaaggtcaag tacaagaaca gcatcttggg cttcatctgg tcgctgctca
     2581 acccgctgat gtacctggtg gtcttctcgt tggtgttcaa catcatcagc gcgacgccgg
     2641 tgccgtacta cgggatcttc ttcctctcgg gcctgctggc ctggaacctg ttcaacaccg
     2701 cgctgggaca gggcacctcg gccatcacga gcaacgccca gctcgtcacc aaggtgtggt
     2761 tcccgcgtga ggcgctggtg ctcgcctcgg tcttctccgc cctcgtgcac ttcttcctgc
     2821 agagcatcgt gctcctgttc gccctgctcg ccttccaacg atcgccggac tactcgttcc
     2881 tcccgatctt cttgttcgcc ctcgtggtgc tgatgctctt cgcgacggcg ctggccgtcg
     2941 gtctcagcgc ggtgaacgtg tacctgcggg acacgcagca cctgctggag gtcgccctgc
     3001 tcggctggtt ctggctgtcc gccgtcgcct acccgttcgg gctcgtgcag acgaagctcg
     3061 aagacaagtg gggggccggc gccgaatggc tcgccggcct gaacccggtg ctcacggtgg
     3121 tcatcacgtt ccagcgcacg ctgtacaacc ccgactactc gggcaccagc acctcggcgg
     3181 tgaacgaggc caacagcctc ccgtaccacc cgtggacctg gtacatgacg ctgctgggcg
     3241 gagcgctcgc cgcctcgctc gtcttgctct tcggctccct ctggctgttc agcaagctcg
     3301 aggacaactt cgcggaggag atctgatggg cgagctcgcc atcgaggtcg ccaacgtcac
     3361 caagtcgttc aaggtgtacc aggagggcgg ccagtcggcg aaggagcggc tgatccgcgc
     3421 cgggcgcaac ccgcacatcg tgttcaaggc gctcgacgac gtgtcgttcg aggtcaagca
     3481 gggcgagacg ttcgccctgc tcggccacaa cggctcgggc aagtccacga tgctcaagtg
     3541 catcgccggc accctccgcc cgagcaccgg caaggtcgtc acccggggcc ggctggctgc
     3601 cctgctggag ctcggcgccg gcttccaccc cgacctcacc ggccgggaga acatctacct
     3661 caacggctcc atcctcggct tctcgaagac gcagatcgac gtgatcttcg acgacatcgt
     3721 cgagttcgcc gaactgagcg acttcatcga ccagcaggtc aagcactact cctcgggcat
     3781 gtacgcccgg ctcgggttct cggtggccat caacgtggag cccgagatcc tgctggtcga
     3841 cgaggtgctg tcggtgggtg acgaatcctt ccagcgcaag tgcatcgacc gcgtccgcca
     3901 gctccagggc gacggccgca cgatcctgct ggtgtcgcac gccgccgagc tggtccgaca
     3961 gatcgccgat cgcgccgccg tgctcgacca cggccacctc gtcgacatcg ccgagcccgg
     4021 cgaggccatc cggacgctgc gcgagacgct cgcccgccgc ggcatcgccc tgctggccga
     4081 agacgaggac gtcaccgagc ccccgcccgc gccgaagtac gccgatccgc cgtcgctcac
     4141 cgtgccggtc gtgaaggtcg acaaggagct cgtcatcacc aaggtggtgg ccgagtaccc
     4201 cgaccccgag gccaagttcc tgctgccgaa ccagccgatg cggctgcgca tcgactactt
     4261 cgccccgcag cgcgtcaccg acatctgcat ggcgttcgag ttccaggacc tccacggcaa
     4321 cgtcatcttc ggcgacgaca cccagaccat ggagcaaccc atccacgcgt gcgacggcgt
     4381 cggcgccgtc tgcttcgacc tgccccgcgt gccgctgctc gacggcgcct acctgatctc
     4441 ggtcaccgcc accacgcgca acggcggcca ggtgtacgac cgccgggacc tccaggaccg
     4501 cttcgaggtc atgcagcccg gccgccagcg gggcaccgtc gcgctcgagc cgaaggtcgt
     4561 ccacttcttc cacgagccgt ccgtgggcga ggacgcgccc gaagccgagg gctccgaccc
     4621 ggcagccggc cccggcgtcg ccaccacgag ttgagcacca ccgaggcggc cgtcgaggcg
     4681 ccgcccgtcg gtggtgccgg cttcgcctac cggccggggc tgacccacct ccgggcgctc
     4741 tcggtggtgc tggtcctgct gttccacgcc ggcgtcgtga agtcgggcta catcggcgtc
     4801 gacgtcttct tcgtcctgtc cgggttcttg atctccaaca tcctctggga gaagctgtcg
     4861 gggagcgacc cggcgcgcca ggtgctcggc agcttctacg cccggcgctg cctgcgcatc
     4921 ctcccgctgt cgctgctggt gctggcggtc accgcggtgg cctcgcacct cttcttcgag
     4981 acgctcatcg acaactggac ctcggcgggc cgggcggcgc tggtctggtc ggagaactgg
     5041 ttcttgatcc gtgagtcgaa cgactacttc gcgccgaccg gcacgaaccc gttccagcag
     5101 tactggtcgc tcggcgtcga ggagcagttc tacgtcgtcc tcccgctgct gatgatgttc
     5161 ctgctgttcg tgctgcgggg gttcaaccgc cgggcccggc tcatcggcct gaccgtcgcc
     5221 gcgggcgtcg gcgtggcggc cgccctggcc agctcgcacc tgctggacat gtcggagtca
     5281 gagacctact actcgtcgtt cgtccggtcg taccagctcc tggccggcgt gttgatcatg
     5341 gcggccgtcc gggtgttcaa gctccgcgac gaccgccgct ggctgttcgc catcg
//
LOCUS       DATMFS010000593         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_251295, whole genome shotgun sequence.
ACCESSION   DATMFS010000593 DATMFS010000000
VERSION     DATMFS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251295"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VNQ33_11335"
     CDS             complement(<1..607)
                     /locus_tag="VNQ33_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98744.1"
                     /translation="MSGLALPRRSERPELVFLSHAVDRSGPPIYLRHLLGWLGANTDV
                     PTRVVSLEGGALEPEFQALTRLQVLGEPDPVRVRRGSRPLTRARNALRGARLRDVHPR
                     SLLYINTAWSVRAMQYVPDHPGPVVAHVHELEVGLDYHLPDDDTDRLFRRPDHWVAAS
                     AAVADNLADRWDVPREEILVHHEMIDVAAPAQVADDEVARLR"
     gene            complement(604..1782)
                     /locus_tag="VNQ33_11340"
     CDS             complement(604..1782)
                     /locus_tag="VNQ33_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98745.1"
                     /translation="MTTGDRPLQVTWLGHGAERTGPPIYLLRILEGLQAHPEIEPSVV
                     LLDDGPLLPELAALAPTTVVRPPPLTGLRRAATAGLSRLGASRSAAALAEGRTGDAAP
                     GPDVVVVNTAGSIAALPALGAPPRRLISHVHELATGLDYWLAPELRRQVLEDSDEIWV
                     VADAVGRHLVEDHGVDAARLRTHRGVLPSDAFTPLDPAERDRRRADLGVPPGHLLVGA
                     SGTLDWRKASDHFLEAAHRTAASAVPIHLVWIGGDPSTPWGRLLVEQAEAAGLADRVR
                     LIAEGDRPDRWFGALDLFLLPSREDAYPLVCMEAAAAGVPLVAFDSGGAGELVRDGGG
                     VVVPYPDAAALAAEVERFARDEAGRRAAGDAARAAAERHTIEAALPSLIADLQRVAAS
                     "
     gene            complement(1779..2966)
                     /locus_tag="VNQ33_11345"
     CDS             complement(1779..2966)
                     /locus_tag="VNQ33_11345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98746.1"
                     /translation="MTRPHVVFLGHGAELTGPPILLGHLVAGLAATGGYDLTVITARS
                     GPLLDQYAASARTSSLGPSREPFGRTSAALRRIGAERTVAPLQDWSRRRLTRKAPPAD
                     LVYVNGATPPTADLLRALDPPRSTPVVLHVHELDIGLRLNLDDDRRRMLFDRADHVIA
                     ASGAVARLLVGGHGLAEDRLTTCPEFVDTAAIHPLAPEAARATAGIPVDALVVGSVGL
                     PDWRKDPDHLLRAVQHLRTIHPALDPWVLWVGGEPASADGRRAADEARRLGLADRFRH
                     VAHQDRPDRLLGALDVFALPAREDALPLAALEAGAAGLPIVCFRTGGVADLCDLGAGT
                     TVAYPDTAAFAGAVAALLANPAERARVGAAAQALVRSQHDSAVGVARIQAVVDATLEA
                     AGR"
     gene            complement(3042..4196)
                     /locus_tag="VNQ33_11350"
     CDS             complement(3042..4196)
                     /locus_tag="VNQ33_11350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98747.1"
                     /translation="MADPHVVVLGHAADRTGPPMYLLELLRGLDRSGLRFTVVLLRGG
                     ELLGELQDLAEVRVVGEPVDTAVARPQRRAEEPARVRARRAQLADLDDVDLAIVNTAW
                     SIQALPWLPEPPRRLVSVVHELGAGVAGLLDRPALAALLASDGFVAGCSAVAAMLEDD
                     LGVAAHRIDLIPYGVAQRPVDLAPVDRTQLDAADDAFLVVAAAVPDRRKGPDLFVHLA
                     AAARRRRPDIAWAFRWIGAADDDPRLADALADRALLGAGDAVRFVPPTPDLRNVLAAG
                     DAFVLPSREDAFPLAAVEAALAGLPLLCFASGGIGDLVEPDGGALVPYPDLDAMADVL
                     AVWHDVPATRSSAGAAARDRARRNHDAADHARAFDAAVLRGLHRPPVPSG"
     gene            complement(4189..5229)
                     /locus_tag="VNQ33_11355"
     CDS             complement(4189..5229)
                     /locus_tag="VNQ33_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98748.1"
                     /translation="MPRRPPPLLVTGGQLWTATGRLVEQLAAPEPGDPRPDVLVWDER
                     DEDGRWEGRPAVFVVRDRPLRQRSPRAAVLELRSHLWRRRDHPVVFVGHQALDAAPWL
                     VRHRGPRTWLPTVDDRSVLDAGLTVPSAGAGGPHHVVVADLELVRSERLRRLPGHRWS
                     PGFRPQRPVTPARPEILVWGPPDRTQGVDLVGRALASHHGALRTAGARVRWIVPLGAE
                     VPAAEADDLRRAGVDDLIEIEPHADPEAALRPRLEGAAALLVCGRPGAWTAEDATLWT
                     AFRSGARVIGFGPPPPADLALDGGGDLLAFGDVAGMGAALSRAVQTADRLPGPIGLAG
                     LVRDLAGTVSGG"
     gene            5343..>5838
                     /locus_tag="VNQ33_11360"
     CDS             5343..>5838
                     /locus_tag="VNQ33_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HWJ98749.1"
                     /translation="MQIPRLVRARRQIRSPRVAPAAPVTPRSACGSPSAAMYFAPDGQ
                     VRACCVNTEYTLGRIGEQSIREIWEGARIASLRTALEGRDFSLGCQDCEHRIAAGDRA
                     WSTAEQYDEYLGTEPGRHPRRMDFILSISCNLQCVMCNGDLSSSIRIHREKRPPLASP
                     YGDAF"
BASE COUNT          727 a         2081 c         2396 g          634 t
ORIGIN      
        1 cacggaggcg cgcgacctcg tcgtcggcca cctgggcggg cgcggccacg tcgatcatct
       61 cgtggtggac gaggatctcc tcgcgaggca cgtcccagcg gtcggcgagg ttgtcggcca
      121 ccgcggccga cgccgccacc cagtggtcgg ggcgccggaa caggcggtcg gtgtcgtcgt
      181 cgggcaggtg gtagtccagc cccacctcga gctcgtgcac gtgggcgacg acgggacctg
      241 ggtggtcggg gacgtactgc atcgcccgga cgctccacgc cgtgttgatg tagagcagcg
      301 accgcgggtg cacgtcgcgc aggcgggcgc cgcgcagcgc gttgcgggcc cgggtgagcg
      361 gtcggctgcc gcggcggacc cggacggggt cgggctcgcc gaggacctgg aggcgggtga
      421 gggcctggaa ctcgggttcg agcgcgcccc cctcgaggga cacgacccgg gtgggcacgt
      481 cggtgttcgc gcccagccac ccgaggaggt ggcgcaggta gatcggcggg ccgctgcggt
      541 cgacggcatg gctgaggaag accagctcgg gccgctcgga gcggcggggc agggcgagcc
      601 cgctcacgat gccgccaccc gctggaggtc ggcgatcagc gagggcaggg cggcctcgat
      661 ggtgtggcgc tcggccgccg cccgcgccgc atccccggcg gcccgtcggc ccgcctcgtc
      721 gcgggcgaag cgctcgacct cggcagccag ggcggcggca tcggggtagg ggacgaccac
      781 gccgccgccg tcgcggacga gctcgccggc cccgccggag tcgaaggcca cgagcggcac
      841 gccggcggcg gccgcctcca tgcacaccag ggggtaggcg tcctcccgcg acggcaggag
      901 gaagaggtcg agggccccga accaccggtc cggccggtcg ccctcggcga tgaggcggac
      961 ccggtcggcg agacccgcgg cctccgcctg ctcgacgagc aggcggcccc acggcgtcga
     1021 cgggtcgccg ccgatccaga cgaggtggat gggcaccgcg ctcgcggcgg tgcggtgggc
     1081 ggcttcgagg aagtggtcgc tggccttgcg ccagtcgagg gtgcccgacg cgccgacgag
     1141 gaggtggccg ggcggcacgc cgaggtcggc ccgtcggcgg tcccgctcgg cggggtcgag
     1201 cggcgtgaag gcatcggagg ggagcacgcc tcggtgggtg cgcagccgcg ccgcatcgac
     1261 gccgtggtcc tcgacgaggt ggcgcccgac ggcgtcggcc acgacccaga tctcgtcgct
     1321 gtcctcgagg acctggcggc gcagctccgg ggcgagccag tagtcgaggc cggtggccag
     1381 ctcgtggacg tggctgatga gccggcgcgg cggtgcgccc agcgccggga gggcagcgat
     1441 gctgcccgct gtgttcacga cgacgacgtc gggtccgggg gcggcgtcgc ccgtgcggcc
     1501 ctcggccagg gcggcggcgg agcggctggc tccgaggcgt gacaggccgg cggtggcggc
     1561 tcgccgcagg cccgtgagcg gtggcggccg gacgaccgtg gtgggggcca gggcggccag
     1621 ctccgggagg aggggcccgt cgtcgagcaa gacgacgctc ggctcgatct ccgggtgcgc
     1681 ctgcaggcct tcgaggatgc ggagcaggta gatcggaggt ccggtgcgct cggccccgtg
     1741 gcccagccag gtgacctgga gcgggcggtc gccagtggtc atcggccagc ggcctccagc
     1801 gtggcgtcga cgacggcttg gatgcgcgcc acccccacgg ccgagtcgtg ctgcgagcgc
     1861 acgagcgcct gagcggcggc gccgacgcga gcccgctcgg ccgggttggc gaggagcgcg
     1921 gcgacggcgc cggcgaacgc ggcggtgtcc gggtaggcca cggtggtccc ggcgcccagg
     1981 tcgcacaggt cggccacgcc gccggtccgg aagcacacga tcgggaggcc ggcagcgccc
     2041 gcctcgaggg cggcgagcgg gagcgcatcc tcgcgggccg ggagggcgaa gacgtcgagc
     2101 gcgcccagca gccggtcggg ccggtcctgg tgggcgacgt gccggaagcg gtctgccagg
     2161 ccgagccgtc gggcctcgtc ggcggctcgg cggccgtcgg ccgacgccgg ctcaccgccg
     2221 acccagagga cccacggatc gagcgcgggg tggatggtgc ggaggtgctg gaccgctcgg
     2281 aggaggtggt ccgggtcctt gcgccagtcc ggcagcccga cggatccgac caccagcgca
     2341 tcgaccggga tgccggcggt ggcgcgcgcc gcctcggggg cgaggggatg gatcgcggcg
     2401 gtgtcgacga actccgggca cgtggtgagc cggtcttcgg ccaggccgtg tccacccacg
     2461 aggaggcggg cgacggcgcc cgacgcggcg atcacgtgat cggcgcggtc gaagagcatc
     2521 cggcggcggt cgtcgtcgag gttcagccgc aggccgatgt cgagctcgtg gacgtggagg
     2581 accaccggcg tcgagcgcgg gggatcgagg gcccgcagga ggtcggcggt gggcggggtg
     2641 gcgccgttca cgtacacgag gtcggctggt ggggccttcc gggtgagccg gcggcggctc
     2701 cagtcctgga gcggggccac ggtgcgctcg gccccgatcc ggcgcagcgc cgcggaggtc
     2761 cgtccgaacg gctcgcggct cgggccgagg ctcgaggtgc gagccgacgc ggcgtactgg
     2821 tcgaggagcg ggcccgaccg ggcggtgatg acggtcaggt cgtacccgcc cgtggcggcg
     2881 aggcccgcca ccaggtgccc gagcaggatg ggcgggccgg tcagctcggc cccgtgcccg
     2941 aggaacacga cgtgggggcg cgtcacggcc agctccccat ccgcgtcacg tccatcgggc
     3001 gagcttcgcg cagcgcgggc gcggcccggc ggtcccggcg gtcacccgga cggcacgggc
     3061 gggcggtgga ggccgcgcag caccgcggcg tcgaaggccc gagcgtggtc ggccgcatcg
     3121 tggttccgtc gggcccggtc ccgcgctgcg gctcccgccg acgaccgggt cgccgggacg
     3181 tcgtgccaca cggccagcac gtcggccatg gcatcgaggt cgggataggg gacgagggcc
     3241 ccgccgtcgg gctcgaccag gtcgccgatg cccccggatg cgaagcacag cagggggaga
     3301 cccgcgagcg ccgcctcgac ggcggcgagg gggaaggcgt cctcccgcga tgggaggacg
     3361 aaggcgtcgc cggcggcgag cacgttgcgc aggtcggggg tgggcgggac gaaccggacc
     3421 gcatcgccgg cgcccagcag cgcccggtcg gcgagcgcat cggccaggcg ggggtcgtcg
     3481 tcggcggccc cgatccaccg gaaggcccag gcgatgtcgg ggcgacgacg ccgagcggcg
     3541 gcagccaggt gcacgaacag gtcggggccc ttgcgccggt cggggacggc cgccgccacc
     3601 accaagaagg cgtcgtcggc ggcgtcgagc tgggtccggt ccaccggcgc gaggtccacc
     3661 ggtcgttgcg ccacgccgta ggggatgagg tcgatccggt gagcggcgac gcccagatcg
     3721 tcctcgagca tcgcggccac cgcggagcac ccggcgacga acccgtcgct ggcgagcagg
     3781 gcggcgaggg cgggacggtc gaggaggccg gccaccccgg cgcccagctc gtggacgacg
     3841 ctgaccaggc gcctcggcgg ctcgggcagc cacggaaggg cctggatgct ccacgccgtg
     3901 ttgacgatgg cgaggtcgac gtcgtcgagg tcggcgagct gggcgcgccg ggcgcggacc
     3961 cgtgccggct cctcggcccg gcgctggggc cgggcgacgg ccgtgtcgac cggctcgccg
     4021 acgacgcgca cctcggccag gtcctgcagc tcgccgagca gctcgccgcc gcgcagcagc
     4081 accaccgtga agcgcaggcc ggaccggtcc aggccgcgca gcagctcgag caggtacatc
     4141 ggcggtccgg tccggtcggc ggcgtggccg agcaccacca cgtgcgggtc agccaccaga
     4201 caccgtcccc gccaggtcgc gcaccagccc ggccagcccg atggggccgg ggagccggtc
     4261 tgcggtctgg accgcccggc tcagcgccgc gcccatcccg gcgacgtcgc cgaacgccag
     4321 caggtccccg cccccgtcga gggcgaggtc cgcgggaggc ggcgggccga agccgatcac
     4381 ccgtgcgccg gaccggaacg ccgtccagag ggtcgcgtcc tcggccgtcc aggccccggg
     4441 acgtccgcag accagcaggg cggcggcgcc ctcgaggcgg gggcgcaggg ccgcttcggg
     4501 gtcggcgtgc ggctcgatct cgatcaggtc gtcgaccccg gcgcgccgca ggtcgtcggc
     4561 ctcggccgcc ggcacctcgg ccccgagggg cacgatccac cggaccctcg cccccgctgt
     4621 gcgcagggca ccgtggtgcg acgccagggc tcggccgacg aggtcgaccc cttgcgtgcg
     4681 gtcgggcggg ccccacacca ggatctctgg tcgggcgggc gtgaccgggc gctggggccg
     4741 gaagccgggc gaccaccggt ggccgggcaa ccggcgcagg cgctcggacc gaaccagctc
     4801 cagatcggcg acgacgacgt ggtgggggcc gccggctccg gccgacggga cggtgaggcc
     4861 ggcgtcgagc acggaccggt catcgaccgt gggcagccac gtccgcgggc cccggtgacg
     4921 gacgagccac ggtgcggcgt cgagcgcctg gtggcccacg aagaccaccg gatggtcgcg
     4981 gcgccgccag aggtgcgacc gcagctccag caccgccgca cgcgggctgc gctggcgaag
     5041 gggccggtct cggaccacga acaccgccgg ccgtccctcc caccgcccgt cctcgtcgcg
     5101 ctcgtcccac acgaggacgt cgggtcgcgg atctcccggc tcgggggcgg cgagctgctc
     5161 caccaggcgg ccggtcgccg tccagagctg ccctccggtg acgaggagcg gtggcggtcg
     5221 ccggggcacc cggcgagtct cgcacggcgg cggcgccggg cgggacgggg tccgggccgt
     5281 cgctgccgtg tggcagggga ccggcgggga gtacgctcgg acggcctgga agggggagtg
     5341 ccatgcagat cccgcgcctc gtgcgagcgc gacgccagat ccggtcgccg cgggtcgcac
     5401 cggccgcgcc ggtcacgccc cgttccgcct gcggctctcc cagcgcggcc atgtacttcg
     5461 cccccgacgg tcaggtgcgg gcctgctgcg tcaacaccga gtacacgctc gggcgcatcg
     5521 gcgagcagtc gatccgggag atctgggagg gtgctcgcat cgcgtcgctg cgcaccgcgc
     5581 tcgagggccg ggacttctcg ctcgggtgcc aggactgcga gcaccgcatc gcggccggcg
     5641 accgggcgtg gagcacggcg gagcagtacg acgagtacct gggcaccgag cccggccgcc
     5701 acccgaggcg catggacttc atcttgtcca tctcctgcaa cctgcagtgc gtgatgtgca
     5761 acggcgacct ctcctcgtcg atccggatcc accgggagaa gcgcccgccg ctcgcgtccc
     5821 cctacggcga cgcgttct
//
LOCUS       DATMFS010000594         4833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_66393, whole genome shotgun sequence.
ACCESSION   DATMFS010000594 DATMFS010000000
VERSION     DATMFS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66393"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VNQ33_11365"
     CDS             <1..1165
                     /locus_tag="VNQ33_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98750.1"
                     /translation="TVRVTTKKPWVAFPAVLYASGRLGIMAQKQLDMDKADCATHPIG
                     TGPFSFVSWTREQSLKVRRYPKYWQDAPDGKPYPYLNAIEFRPVQNSDSRIAQLQQGE
                     LNMMHTSTSSDMADTLPSMRDAGAINLLVSSEKTEVAYLMMNSTKAPLDDRGTRLAIA
                     HAIDRDALNEKSNDGFPAVANGPFAPTVLGHLDDPGFPGYDLAAAKAQVAKLKKAKKD
                     TSLSLLTSAGPVAVRQAELEKEMLEAAGFDVSLEIETEADLIDRVISGDYEIAAFRNQ
                     PGEDPDMDYIWWYGEDNPVNFGRFDDPVIDKNLDTGRSDPDRTVRREAYEAINRQFAK
                     QVWNVWLWHSPWAVAEASNVHGILGPDLPGDEGPPSARLVTGHSLLGVWIDQN"
     gene            1296..3056
                     /locus_tag="VNQ33_11370"
     CDS             1296..3056
                     /locus_tag="VNQ33_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ98751.1"
                     /translation="MRQSSRPSGRRVTAAVAAVLALSLMASSCGGTKASDKKSEVKLS
                     EKGLTAKDGESGLKDAGTPQRGGTIVYGVEADSSSGYCLPEGQLAISGMLVVRAIYDT
                     LTVPNNNGEYVPYLAKSVVPNDDFTEWTITVRDGIKFHDGTDLTGKVVQNNIDAYRGA
                     YPARKPLLFTFVLSNIESTSSQGQTVTVKTKVPWVAFPAFLYSSSRMGIMAQAQLDDA
                     KTCDRKLIGTGPFKFKSWTPNDKLVGTRNENYWQTAPDGEPYPYADGIEFRVLTDGTV
                     RINSLKSSDGANIIHTTNAEDIGGQLYDLREEGDVNMLVSEDGAEVSFVQLNASQPPF
                     NDLKMRQALAYGSNRPEINDIQNDGLPTVANSPFGPDSIAYEQDPGFPKYDLEKAKSL
                     VQEYVKGGGKAEFTVTTTSDPDTVRLAELVQQRAEKLGVKVSVIKRDQAALIDDAIGK
                     KYQAMLFRNYPGGDPDINYVWWYGAKTNPVNFGGYDDEVINKLLDEGRSEPDKDKRAE
                     IYKQIGQEFAKQAWSMWSWYTPWAVVEKSNVHNILGPPLPGENPSKPGEETTDDPQYQ
                     PFTGLATGHSLIGLWIDQKN"
     gene            3135..4091
                     /locus_tag="VNQ33_11375"
     CDS             3135..4091
                     /locus_tag="VNQ33_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98752.1"
                     /translation="MLLIGKRILQLVVVFVCVTFFTVMLISMVPGKPEVVVIPFDNPE
                     QTQRQTFREENHLDRPLVVQWGYWAKDFVRGDMGNYYTSTGTNPVRDRIGAALPISLL
                     LILYVQIVSLGIAIPLAVWAAYKSGSRADKLFNAGAFGCISLPGFAVALIGTYILGVQ
                     FNLVPTDGYTSPSDGLLEHVKSMVVPVLALSLGQIAVYMRLLRSDLVATLQEDFILMA
                     KAKGISNRRVLWRHALRPSSLTLLTVAGLSVGTLIGGALVVEYLLRIPGMGSLIGEAV
                     LKRQYVALQSMVALIAIFFVLLNFLIDYLYTVLDPRIRERRS"
     gene            4088..>4833
                     /locus_tag="VNQ33_11380"
     CDS             4088..>4833
                     /locus_tag="VNQ33_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ98753.1"
                     /translation="MTDTTTTNEPGANQAAAVPAVPVAVLDDVKKKSGLGVMGWVSAG
                     WLILIVGAAALADILPLNDPAAEVADKRLAPFQPGGPILGADANGRDLLARTIYGART
                     SLIIAFCAVMIGFIIGGFLGLLAGYFRNWAGNALVSVFDILLAIPQLVLALSLVSVLK
                     GSPEPVEPGQAEPFKLPVTLILIIALGIVSIPLLARITRASTLTWSQREFVMAARAQG
                     ATHGRILFREVLPNVLPAMVSIALLGMAIA"
BASE COUNT          848 a         1785 c         1465 g          735 t
ORIGIN      
        1 gaccgtgcgc gtcacgacga agaagccgtg ggtcgcgttc cccgccgtgc tctacgcgtc
       61 gggccgcctc gggatcatgg cgcagaagca gctcgacatg gacaaggccg actgcgccac
      121 ccacccgatc gggaccggcc cgttctcgtt cgtgtcctgg acgcgcgagc agtccctgaa
      181 ggtccggcgc taccccaagt actggcagga cgctcccgac gggaagccct acccctacct
      241 gaacgccatc gagttccgcc cggtgcaaaa cagcgactcc cgcatcgccc agctccagca
      301 gggcgagctg aacatgatgc acacctcgac ctcgtcggac atggctgaca ccctcccgtc
      361 gatgcgcgac gccggtgcca tcaacctgct cgtgtcctcg gagaagaccg aggtcgccta
      421 cctgatgatg aacagcacga aggcgccgct ggacgaccgg ggcacgcggc tggccatcgc
      481 ccacgccatc gaccgcgacg cgttgaacga gaagtcgaac gacggcttcc ccgccgtcgc
      541 caacgggccg ttcgccccca ccgtgctcgg ccacctcgac gatccgggct tcccgggcta
      601 cgacctggcg gcggccaagg cccaggtcgc caagctgaag aaggcgaaga aggacacgtc
      661 gctctcgctg ctgacctccg cgggccccgt ggccgtgcgc caggcggagc tggagaagga
      721 gatgctcgag gccgccggct tcgacgtgag cctcgagatc gagaccgaag cggacctcat
      781 cgaccgggtc atctccggcg actacgagat cgccgccttc cgcaaccagc cgggcgagga
      841 ccccgacatg gactacatct ggtggtacgg cgaggacaac ccggtgaact tcggccgctt
      901 cgacgacccg gtcatcgaca agaacctcga caccggccgg tccgacccgg accggaccgt
      961 tcgccgcgag gcctacgagg cgatcaaccg ccagttcgcg aagcaggtct ggaacgtgtg
     1021 gctctggcac tcgccctggg ccgtcgccga ggcctcgaac gtccacggca tcctcggccc
     1081 ggaccttccg ggcgacgagg gtcccccgtc cgctcgcctc gtgaccggac actccctgct
     1141 cggcgtgtgg atcgaccaga actgacccgc gggtcaagtc gggccgatgc ggccacggca
     1201 cctctttgcg aacgctatgg tcacggtcac gtcgcttgcc gggagtcaca ttcccgggct
     1261 gcggccgcca cccacaccgc acaccagggg aacccatgcg tcaatccagc cggccgtctg
     1321 gccgccgagt cactgccgcc gtcgccgccg tcttggccct gagcctcatg gcgtcgtctt
     1381 gcggcggcac caaggccagc gacaagaagt ccgaggtgaa gctcagcgag aaggggctca
     1441 ccgccaagga cggcgagagc gggctgaagg acgccggcac cccccagcgc ggcgggacca
     1501 tcgtctacgg cgtcgaggcc gacagctcca gcggctactg cctccctgaa ggccagctgg
     1561 ccatctccgg gatgctggtc gtccgggcca tctacgacac cctcaccgtg cccaacaaca
     1621 acggcgagta cgtcccctac ctcgccaaga gcgtcgtgcc gaacgacgac ttcaccgagt
     1681 ggaccatcac cgtccgcgac ggcatcaagt tccacgacgg caccgacctc accggcaagg
     1741 tcgtccagaa caacatcgac gcctaccgag gcgcctaccc cgcccggaag ccgctgctgt
     1801 tcaccttcgt gctgagcaac atcgagtcga cctcgtcgca gggccagacg gtcaccgtca
     1861 agaccaaggt cccctgggtc gccttcccgg cgttcctcta cagctcgagc cgcatgggga
     1921 tcatggccca ggcccagctc gacgacgcga agacctgcga tcgcaagctc atcgggaccg
     1981 gccccttcaa gttcaagagc tggaccccga acgacaagct cgtcggcacc cgcaacgaga
     2041 actactggca gaccgccccc gacggggagc cctaccccta cgccgacggc atcgagttcc
     2101 gcgtcctgac ggacggcacc gtgcgcatca actcgctcaa gagcagcgac ggcgccaaca
     2161 tcatccacac gaccaacgcc gaagacatcg gtggccagct ctacgacctg cgtgaggagg
     2221 gcgacgtgaa catgctcgtg tccgaggacg gcgcggaggt ctcgttcgtg cagctcaacg
     2281 cgagccagcc cccgttcaac gacctcaaga tgcgccaggc cctggcctac ggctccaacc
     2341 gcccggagat caacgacatc cagaacgacg ggctgcccac ggtcgccaac agccccttcg
     2401 gtcccgactc catcgcctac gagcaagatc ctggcttccc gaagtacgac ctcgagaagg
     2461 ccaagagcct cgtccaggag tacgtcaagg gcggcggcaa ggccgagttc accgtgacca
     2521 ccacgtccga tcctgacacc gtgcgcctgg ccgagctggt ccagcagcgg gccgagaagc
     2581 tcggcgtgaa ggtctccgtc atcaagcgcg accaggcggc gctgatcgac gacgccatcg
     2641 gcaagaagta ccaggcgatg ctgttccgca actacccggg cggcgacccc gacatcaact
     2701 acgtgtggtg gtacggcgcc aagaccaacc cggtgaactt cggcggctac gacgacgagg
     2761 tgatcaacaa gctcctcgac gaaggccgca gcgagcccga caaggacaag cgggccgaga
     2821 tctacaagca gatcggccag gagttcgcca agcaggcctg gagcatgtgg tcctggtaca
     2881 ccccgtgggc cgtggtcgaa aagagcaacg tgcacaacat cctcggcccg ccgctccccg
     2941 gcgagaaccc gtccaagccg ggcgaggaga ccaccgacga tccgcagtac cagcccttca
     3001 cgggcctggc caccgggcac tcgctcatcg gcctctggat cgatcagaag aactgatcct
     3061 tcgtcccccc cactcttcgg aaccgaggcg gctcgtcgca ccagcggcga gccgcctgag
     3121 agaggaccaa tcggatgctg ctgatcggca agcggatcct gcaactcgtc gtggtgttcg
     3181 tgtgcgtcac gttcttcacc gtgatgctca tctcgatggt gccgggcaag cccgaggtcg
     3241 tcgtcatccc cttcgacaac cccgagcaga cccagcgcca gacgttccga gaggagaacc
     3301 acctcgaccg cccgctcgtc gtgcagtggg gctactgggc caaggacttc gtccgtggcg
     3361 acatgggcaa ctactacacg tccacgggca ccaacccggt ccgtgaccgc atcggtgcgg
     3421 ccctgccgat ctcgctcctg ttgatcctgt acgtgcagat cgtgtccctc gggatcgcca
     3481 tcccgttggc ggtgtgggcg gcgtacaagt ccggcagccg ggcggacaag ctcttcaacg
     3541 ccggagcctt cgggtgcatc tcgctcccgg gcttcgccgt ggccctcatc ggcacctaca
     3601 tcctcggcgt ccagttcaac ctggtgccca ccgatggcta cacctcaccg tccgacgggc
     3661 tcctcgagca cgtcaagtcc atggtggtgc ccgtcctggc gctgtcgctc ggccagatcg
     3721 ccgtctacat gcggctgctg cgatccgacc tcgtcgccac gctccaggag gacttcatcc
     3781 tcatggccaa ggccaagggc atctccaacc gccgggtgct ctggcgccac gcgctgcggc
     3841 catcgagcct cacgctgctc accgtcgccg gcctcagcgt cggcaccctc atcggcggtg
     3901 cgctcgtggt ggagtacctg ctccggatcc cggggatggg ctcgctcatc ggcgaggccg
     3961 tgctcaagcg ccagtacgtg gcgctccaga gcatggtggc cctcatcgcc atcttcttcg
     4021 tcttgttgaa cttcttgatc gactacctgt acacggtcct cgatccccgg atccgggagc
     4081 gccggtcatg accgacacca cgacgacgaa cgaacccggc gccaaccagg cggccgcggt
     4141 gcctgcggta cccgtcgcgg tcctcgacga cgtcaagaag aagtccggcc tcggcgtcat
     4201 gggctgggtc tccgccggct ggctcatctt gatcgtcggc gccgccgccc tggccgacat
     4261 cctccccctc aacgaccccg ccgcggaggt cgccgacaag cggctggccc ccttccagcc
     4321 gggcgggccc atcctcggcg ccgacgccaa cggccgcgac ctgctggctc gcaccatcta
     4381 cggcgcccgc acgtcgctca tcatcgcctt ctgcgccgtg atgatcggct tcatcatcgg
     4441 cgggttcctc gggctcttgg ccggctactt ccgcaactgg gccggcaacg ccctcgtgag
     4501 cgtgttcgac atcctcctcg ccatccccca gctggtgctg gcgctgtcgc tggtgtcggt
     4561 gctcaagggc tcgcccgagc cggtggagcc gggacaggca gagccgttca agctgccggt
     4621 cacgctgatc ttgatcatcg ccctcggcat cgtctcgata cccctgctcg cccggatcac
     4681 gcgcgcctcg acgctcacgt ggtcccagcg cgagttcgtg atggccgccc gggcgcaggg
     4741 agccacccac gggcgcatct tgttccgcga ggtcctgccc aacgtcctcc cggccatggt
     4801 gtccatcgcc ctgctcggca tggcgatcgc cat
//
LOCUS       DATMFS010000595         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_323653, whole genome shotgun sequence.
ACCESSION   DATMFS010000595 DATMFS010000000
VERSION     DATMFS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323653"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /gene="atpA"
                     /locus_tag="VNQ33_11385"
     CDS             <1..1413
                     /gene="atpA"
                     /locus_tag="VNQ33_11385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWJ98754.1"
                     /translation="ASVGGLPDCAVNEMLEFEDGTIGLALNLDEESIGAVVLGEVGSI
                     EEGQVVRATGKILSVPCGDGLLGRVVNTLGEPVDGKGPLTNVSTRRMEVQAPGIMGRK
                     PVHEPLQTGIKAIDAMTPIGRGQRELIIGDRKTGKTSVAIDTILNQKGLGVNCVYVAI
                     GQKASTVAQTVATLEERGAMEYTVVVVSPASDPAPFKYLAPYTGCAMGQHWMENGQHA
                     LAVYDDLSKQAEAYRSVSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDANGAGSMT
                     ALPIIETKAGDISAYIPTNVISITDGQVFLEEDLFKSGIRPAVNVGQSVSRVGGAAQI
                     KAMKTAVGTLKGDLAQFRELEAFASLGSELDAVSQATLDRGRRLVELLKQGLNSPLPV
                     EDQVVVLYAGTRGYLDPIPVEDVQDYESRLLEWLKARFGGLLAQIRDEGTLDEDGLKA
                     ALADFANEFGTGSAAAADEA"
     gene            1422..>2121
                     /gene="atpG"
                     /locus_tag="VNQ33_11390"
     CDS             1422..>2121
                     /gene="atpG"
                     /locus_tag="VNQ33_11390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HWJ98755.1"
                     /translation="MASGQERVLRRRIKSVQSTKKITKAMELISASRIAKAQERVAAA
                     RPYSERITDVIRNLAAAGAGGDSPLLNPRAEVTTVAYVVVAADRGLAGGYNSAVIRAA
                     ERAMAADQEAGRQTALITVGKKATGYFRFRGWEIAQSFVGFSDAPTYEDARQVAGHVA
                     EKFESGEYSEVHLAYTQFLSMGTQIPQVVQYMPLDTSSIDDAKASGPSADYEFEPNPS
                     EILDRLLPRYAEARL"
BASE COUNT          344 a          782 c          705 g          290 t
ORIGIN      
        1 gcctccgtgg gcggcctgcc ggactgcgcg gtcaacgaga tgctcgagtt cgaggacggg
       61 accatcggtc tcgccctgaa cctcgacgaa gagtccatcg gcgccgtggt gctcggcgaa
      121 gtgggcagca tcgaggaggg ccaggtggtc cgggcgaccg gcaagatcct ctccgtgccg
      181 tgtggcgacg gcctcctggg ccgcgtggtg aacacgctgg gcgagcccgt cgacggcaag
      241 ggcccgctca ccaacgtgag cacccgccgc atggaggtcc aggctccggg catcatgggc
      301 cgcaagcccg tgcacgagcc gctccagacg ggcatcaagg ccatcgacgc catgacgccc
      361 atcggccggg gccagcgcga gctgatcatc ggcgaccgca agaccggcaa gacgtcggtg
      421 gcgatcgaca ccatcttgaa ccagaagggt ctgggggtga actgcgtgta cgtggccatc
      481 ggccagaagg cgtccaccgt cgcccagacg gtggccacgc tcgaagagcg cggcgccatg
      541 gagtacacgg tcgtcgtcgt ctcgccggcc tcggacccgg cgccgttcaa gtacctggcc
      601 ccctacaccg gctgcgccat gggccagcac tggatggaga acggccagca cgccctcgcc
      661 gtctacgacg acctgtcgaa gcaggccgag gcctaccgct cggtgtcgct gctgctgcgc
      721 cgcccgccgg ggcgcgaggc ctacccgggc gacgtgttct acctgcacag ccggctgctc
      781 gagcgggccg ccaagctgtc cgacgccaac ggcgccggct cgatgacggc cctgccgatc
      841 atcgagacca aggccggcga catctcggcg tacatcccca ccaacgtgat ctcgatcacc
      901 gacggccagg tgttcctcga agaggacctg ttcaagtccg gcatccgccc ggccgtgaac
      961 gtcggccagt cggtgtcgcg agtcggtggc gccgcccaga tcaaggccat gaagaccgcc
     1021 gtgggcacgc tcaagggcga cctcgcccag ttccgtgagc tggaggcgtt cgcctcgctc
     1081 ggctccgagc tcgacgccgt gtcgcaggcc accctcgacc gcggccgccg cctggtggag
     1141 ctcctcaagc agggcctcaa ctcgccgctg cccgtcgagg accaggtcgt ggtgctctac
     1201 gccggcaccc ggggctacct cgacccgatc ccggtcgaag acgtccagga ctacgagagc
     1261 cgcctgctcg agtggctgaa ggcccgcttc ggcggcctcc tggcccagat ccgcgacgag
     1321 ggcaccctcg acgaggacgg cctcaaggct gccctcgccg acttcgccaa cgagttcggc
     1381 accggctccg ccgctgccgc cgacgaggcc taggagacgc cgtggccagc ggccaggaac
     1441 gggtcctccg ccggaggatc aagtccgtcc aatccaccaa gaagatcacc aaggcgatgg
     1501 agctgatctc ggcttcgcgc atcgccaagg cgcaggagcg cgtggccgcc gcacgcccct
     1561 acagcgagcg gatcaccgac gtgatccgca acctcgccgc cgctggtgcc ggtggggact
     1621 cgccgctgct caacccgcgc gccgaggtga ccacggtggc ctacgtcgtc gtcgctgccg
     1681 accgcggcct cgccggcggc tacaactcgg cggtcatccg cgccgccgag cgggccatgg
     1741 ccgcggacca ggaggccggt cgccagaccg cgctcatcac ggtcggcaag aaggccaccg
     1801 gctacttccg cttccgcggc tgggagatcg cccagagctt cgtcgggttc tccgacgctc
     1861 ccacctacga ggatgctcgc caggtggcgg gccacgtggc cgagaagttc gagtcgggtg
     1921 agtactccga ggtccacctc gcgtacacgc agttcctgag catgggcacc cagatcccgc
     1981 aggtcgtgca gtacatgccg ctcgacacgt cgtccatcga cgacgccaag gcctccggcc
     2041 ccagcgccga ctacgagttc gagccgaacc cgagcgagat cctcgaccgg ctcctgcccc
     2101 gctacgccga ggcccgcctc t
//
LOCUS       DATMFS010000596         5346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_90686, whole genome shotgun sequence.
ACCESSION   DATMFS010000596 DATMFS010000000
VERSION     DATMFS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90686"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VNQ33_11395"
     CDS             complement(<1..585)
                     /locus_tag="VNQ33_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWJ98756.1"
                     /translation="MKLDDVDGDDFQIEDRRGQGPSGGGLGGLGSVLGGGGKGAKLGI
                     PALIVVVIGIFLSQAGGGGGSSSGGLDDILGQLSGNGSTQQTAPSNPAGTKDSQYEFV
                     GDVGSLLEDYWSSEFAASNQDFSKASLVIFDAPTDTGGCGVGQPQAGPFYCPPSKRIF
                     IDFGFYEQLEQQLGFDGDFAMAYVIAHEYGHHVQN"
     gene            complement(659..1345)
                     /locus_tag="VNQ33_11400"
     CDS             complement(659..1345)
                     /locus_tag="VNQ33_11400"
                     /inference="COORDINATES: protein motif:HMM:NF039921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWJ98757.1"
                     /translation="MTANPDATAADPEAEPRAADGRVPGRRGQATRQKLLDCAADMLR
                     TTSYRDLKVVDIAREAGTSPATFYQYFPDVESAILVLADEMVASGTEQFADLVTANSW
                     KGKAGYEGAEALASAMLTFWAEHQPVLRVIDLATDEGDPRFANIRIHLLNDLNNALAQ
                     AAKEHQKAGKGQPGIDSEAIAGVLVAMLAHVSAHRLGFELWGVRADDLQATMARIVFW
                     TVTGQKPPAT"
     gene            complement(1355..2155)
                     /locus_tag="VNQ33_11405"
     CDS             complement(1355..2155)
                     /locus_tag="VNQ33_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWJ98758.1"
                     /translation="MITPSLLADASGDLDGQYLGVLLIRVVIGLTLAAHGYYKFFKGG
                     KIAGTARWFDSIGMRPNGTVHALMAATTEIGAGLLFAVGLLTPFAAAGFVGLMFVAAW
                     TVNRPNGFFSANHGYEFNLTLAVVAVGVAAIGPGRYSLDWQFDLAFPFQPKAAVAIAA
                     GLGLVAGIGLVAACFRPPPPAPADPVAANDEPVITLDDEPPAVTDGEDPTLVLDDVTP
                     PLDDDAVLALDDVPAAPEGATLALDDHDDHDDPGDDDAGLALDDERDA"
     gene            complement(2152..3801)
                     /locus_tag="VNQ33_11410"
     CDS             complement(2152..3801)
                     /locus_tag="VNQ33_11410"
                     /inference="COORDINATES: protein motif:HMM:NF005801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HWJ98759.1"
                     /translation="MTDQPAPADVDPDGDDPRADLRWGTIPAALAGAVARGGSSEALV
                     DGDTSWSWERIGREVDRAAKAYIAAGIEIGDRVAIWAPNCAEWVVALLGLQSAGAVLV
                     PLNTRFKGVEAADIIGRSRARILVTVDGFLDNRYVAMLDGHDLPHLERIVVLRTEPGH
                     DLPSGAERWEDFVGSGEADAVTDLSVAARRAQLDASSVADLLFTSGTTGKPKGVICTH
                     GQTLRTVATWANVVGLDETDRYLAINPFFHSFGYKAGIVSWLVTGCVLVPMPVFDVPK
                     AMELITAQRISMVPGPPTLYQTILNHPGRGRLDSSSLRLAVTGAASVPVSLVEQMHDD
                     LGFDTVITAYGLTEGCGFATMCRRTDDATTIATTSGRAMPGIEVHVVDEAGTPTPAGE
                     PGEVVVRGYNVMQGYFENPEATAEAVDADGWLHTGDIGVMDERGYLRITDRMKDLFIV
                     GGFNAYPAEIENLLLAHPHIAQVAVIGVPDERMGEVAAAFVVPASGTTPDPDEIMAWA
                     RDAMANYKAPRQVHVVDALPFNAGGKVMKFQLRELLQEDSP"
     gene            complement(3798..4436)
                     /locus_tag="VNQ33_11415"
     CDS             complement(3798..4436)
                     /locus_tag="VNQ33_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWJ98760.1"
                     /translation="MPAPSLASLVAPGSTAIVTSEVQSGVVGPQSALPALAAAAAPVI
                     ERLGTLLPAARAAGVRVIHATASRRADAAGSNQNARLFLAVRKSPVALLPGSRESQVV
                     PELGPEPEDLVLNRLHGLNPMAGTDLDPILRNLGVSTLVVTGVSVNVAVTNLVMDAVN
                     KGYQVVLPRDGVCGLPAAYADAVIDNTLSLLATVTTVDDLVAAWAPTTEEPS"
     gene            4585..>5346
                     /locus_tag="VNQ33_11420"
     CDS             4585..>5346
                     /locus_tag="VNQ33_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HWJ98761.1"
                     /translation="MAEYEPFPTGSGPVEHDRDLTKKVLGCFGLGSLGVIGAVVAVMV
                     LLGALASFASGCDFEVGDPGDGKDSQRLTVGVAPKTGLSDATAVRVTSTAFQPDTIVG
                     VAVCLRAADTERKGVEACDENQGSRFATTAEGELDATYAVPRVITVGGTAYDCAAEAG
                     ACLVVAADASDYDQSGGQPVTFRSGLPAADLTPVTVRPVSDHLPIGAQPPVGSTPVAA
                     GTGLKVLASGFQPGEPLLIAHCTSKVEEDGIVGTCE"
BASE COUNT          757 a         1814 c         2016 g          759 t
ORIGIN      
        1 gttctggacg tggtggccgt actcgtgggc gatcacgtag gccatggcga agtcgccgtc
       61 gaagccgagc tgctgctcga gctgctcgta gaacccgaag tcgatgaaga tcctcttcga
      121 gggcgggcag tagaacggcc cggcctgcgg ctggccgacc ccgcacccgc ccgtgtcggt
      181 gggggcgtcg aagatcacga gcgacgcctt cgagaagtcc tggttcgacg ccgcgaactc
      241 actcgaccag tagtcctcga gcagcgagcc gacgtcgccg acgaactcgt actggctgtc
      301 cttcgtcccc gcggggttcg acggcgcggt ctgctgcgtc gagccgttcc cgctgagctg
      361 gccgaggatg tcgtcgaggc cgcccgagct gctgcctccc ccgccgcccg cctgcgagag
      421 gaagatgccg atcaccacga cgatgagcgc ggggatgccg agcttggccc ccttgccccc
      481 gccgcccagc accgacccga gaccgccgag gccaccgccg ctgggcccct ggccgcgccg
      541 gtcctcgatc tggaagtcgt cgccatcgac gtcatcgagc ttcacggaac cgcatcgtag
      601 gaccccgtcc tgtcgcgtcg ggccgccaac cctcgacacg gccgatgcgc cggcgccgtc
      661 aggtggcggg gggcttctgg ccggtgacgg tccaaaagac gatgcgggcc atggtggcct
      721 gcaggtcgtc ggcccgaacg ccccagagct cgaagccgag gcggtgcgcc gacacgtggg
      781 cgagcatggc caccagcaca ccggcgatgg cctcggagtc gatgcccggc tggcccttgc
      841 cggccttctg gtgctccttg gccgcctggg ccagggcgtt gttcaggtcg ttgagcaggt
      901 ggatgcggat gttggcgaag cgggggtcgc cttcgtcggt ggccaggtcg atgacccgca
      961 gcacgggctg gtgctccgcc cagaacgtga gcatggcgct ggccagcgcc tcggcgccct
     1021 cgtagccggc cttgcccttc cacgagttgg cggtgacgag gtcggcgaac tgctcggtgc
     1081 cactggcgac catctcgtcg gcgaggacca ggatcgccga ctccacgtcg gggaagtact
     1141 ggtagaaggt cgccggcgag gtaccggcct cccgggcgat gtcgacgacc ttgaggtccc
     1201 ggtacgaggt ggtgcgcaac atgtcggcgg cgcagtcgag gagcttctgg cgggtggcct
     1261 ggccgcgccg gccgggaacg cggccatcgg cagcgcgcgg ctctgcctca ggatcggcag
     1321 ccgtggcgtc ggggttcgcg gtcacggcga gaggttacgc gtcgcgctcg tcgtccaacg
     1381 ccagtccggc atcgtcatca ccgggatcgt cgtggtcgtc gtggtcgtcg agcgccaggg
     1441 tggcgccctc gggagcggcg gggacgtcgt cgagcgccag cacggcatcg tcgtcgagcg
     1501 ggggcgtgac gtcgtcgagg acgagggtgg ggtcctcgcc gtcggtgacg gcgggcggct
     1561 cgtcatcgag cgtgatcacc ggctcgtcgt tcgccgcgac aggatcggcc ggagccgggg
     1621 gcggcggtcg gaagcaggcg gccacgaggc cgatgccggc gaccagcccg aggccggcgg
     1681 cgatggcgac cgccgccttc ggctggaacg ggaaggccag gtcgaactgc cagtcgagcg
     1741 agtacctccc cgggccgatg gcggcgacgc cgacggcgac gacggcgagc gtgaggttga
     1801 actcgtagcc gtggttggcg ctgaagaacc cgttgggccg gttgaccgtc caggccgcca
     1861 cgaacatgag gcccacgaag ccggcggcgg cgaagggcgt gaggaggccg acggcgaaca
     1921 agagcccggc gccgatctcc gtggtggccg ccatcagggc gtggaccgtg ccgttcggcc
     1981 tcatgccgat ggagtcgaac caccgggcgg tgccggcgat cttcccgccc ttgaagaact
     2041 tgtagtagcc gtgggcggcg agggtgaggc cgatgacgac ccggatgagc aagacgccga
     2101 ggtactggcc gtcgaggtct ccgctggcgt cggcgagcag cgagggcgtg atcatgggct
     2161 gtcctcctgg aggagctcgc ggagctggaa cttcatgacc ttgcctccgg cgttgaacgg
     2221 cagcgcatcg acgacgtgga cctgacgggg cgccttgtag ttggccatcg cgtcgcgcgc
     2281 ccacgccatg atctcatctg gatcgggcgt ggttccggat gctggaacga cgaacgcggc
     2341 ggcgacctcg cccatgcgct cgtcggggac gccgatcaca gcgacctggg cgatgtgcgg
     2401 gtgggcgagc agcaggttct cgatctcggc cgggtaggcg ttgaagccgc cgacgatgaa
     2461 gaggtccttc atccggtcgg tgatgcgcag gtagccgcgc tcgtccatca ccccgatgtc
     2521 gccggtgtgc agccacccgt ctgcgtcgac ggcctccgcc gtggcctcgg ggttctcgaa
     2581 gtagccctgc atgacgttgt agccccggac gacgacctcg ccgggctccc ccgcgggcgt
     2641 cggcgtgccg gcctcgtcga cgacgtgcac ctcgatgccg ggcatggccc ggcccgacgt
     2701 ggtggcgatg gtcgtggcgt cgtcggtgcg gcggcacatg gtggcgaagc cgcacccctc
     2761 ggtgagcccg taggccgtga tcaccgtgtc gaagcccagg tcgtcgtgca tctgctcgac
     2821 caggctgacc ggcaccgagg ccgcacccgt gaccgccagg cgcaggctcg acgagtcgag
     2881 ccggccgcgg ccggggtggt tgaggatcgt ctggtagagc gtcggcgggc ccgggaccat
     2941 cgagatgcgc tgggcggtga tcagctccat cgccttgggc acgtcgaaca ccggcatcgg
     3001 cacgagcacg cacccggtca cgagccagga gacgatcccg gccttgtacc cgaacgagtg
     3061 gaagaacggg ttgatcgcca ggtagcggtc cgtctcgtcc aggcccacga cgttggccca
     3121 ggtggcgacg gtgcgcaggg tctggccgtg cgtgcagatg acgcccttgg gcttgccggt
     3181 ggtgccggac gtgaagagca ggtcggccac ggaactggcg tcgagctggg cgcgacgggc
     3241 agcgacggac aggtcggtga cggcgtcggc ctcgccggag ccgacgaagt cctcccagcg
     3301 ctcggcgccg gacggcaggt cgtggccggg ctcggtgcgc agcaccacga tccgctcgag
     3361 gtgcggcagg tcgtgcccgt cgagcatggc gacgtagcgg ttgtcgagga acccgtcgac
     3421 cgtcaccagg atgcgggcgc ggctgcggcc gatgatgtcg gcggcctcga cgcccttgaa
     3481 ccgcgtgttg agcgggacga gcacggcgcc ggcggactgc aggccgagga gggcgaccac
     3541 ccactcggcg cagttcggcg cccagatggc cacccggtcg ccgatctcga tgcccgccgc
     3601 gatgtacgcc ttggcggccc ggtcgacctc ccggccgatg cgctcccagc tccacgacgt
     3661 gtccccgtcg acgagggcct cgctcgaacc gccacgggcg acggcaccgg cgagcgcggc
     3721 ggggatggtc ccccagcgga ggtcggcgcg ggggtcgtcg ccgtcgggat cgacgtcggc
     3781 gggggcgggc tggtcggtca cgacggctcc tccgtggtgg gcgcccacgc cgccaccagg
     3841 tcgtcgaccg tggtgaccgt ggcgaggagc gacagcgtgt tgtcgatgac ggcgtcggcg
     3901 taggcggcgg gcagtccgca gacgccgtct cggggcagga ccacctggta gcccttgttc
     3961 accgcatcca tcaccaggtt cgtgacggcg acgttgaccg acaccccggt gacgacgagc
     4021 gtcgagacgc ccaggttgcg caggatcggg tccaggtcgg tgcccgccat cgggttgagg
     4081 ccgtgcagcc ggttgagcac caggtcctcg ggctcggggc ccagctccgg caccacctgc
     4141 gactcccgcg acccgggcag cagggcgacc ggggacttcc ggacagcgag gaagagccgg
     4201 gcgttctggt tggacccggc cgcgtcggcg cggcgcgacg cggtggcgtg gatgacccgg
     4261 acgcccgccg cacgcgccgc cgggagcagg gtgccgagcc gttcgatgac cggagcagcg
     4321 gccgccgcga gcgccggcag cgccgactgc ggtccgacca cgccgctttg cacctcggag
     4381 gtgacgatgg cggtggaacc aggagcgacg agcgacgcga gcgacggtgc aggcacggct
     4441 ggcgagcgta tctgacggac cgtcaggacg aggcgggggc gcgcggacac ccaggtgctc
     4501 cccgccgtaa cgtcgccatc agcaagcggg ggaccgtcgg tggcgcaggg gaaccggtgc
     4561 cacctcgaac cagcgaggtg ttccatggcg gagtacgagc ctttccccac cggttccggc
     4621 ccggtcgagc acgatcggga cctcacgaag aaggtcctcg ggtgcttcgg cctcggcagc
     4681 ctgggcgtga tcggcgccgt cgtcgccgtg atggtcctgc tcggagcgct ggcgtcgttc
     4741 gcctccggtt gcgacttcga ggtcggcgac cccggcgacg gcaaggactc gcagcgcctc
     4801 accgtcggcg tggcgcccaa gacgggcctg agcgacgcca ccgcggtccg ggtcacgagc
     4861 acggccttcc agcccgacac catcgtcggt gtcgccgtgt gcctgcgcgc cgcggatacc
     4921 gagcgcaagg gcgtcgaggc gtgcgacgag aaccagggct cgcggttcgc cacgaccgct
     4981 gagggcgaac tggacgccac ctacgccgtc ccccgggtca tcaccgtcgg tgggacggcg
     5041 tacgactgcg ccgccgaagc cggggcgtgc ctggtcgtgg cggctgatgc cagcgactac
     5101 gaccagtcgg gcggacagcc ggtcacgttc cgctcggggc tccccgccgc cgacctcacg
     5161 ccggtcaccg tgcggccggt ctcggaccac ctcccgatcg gtgcccagcc accggtcggg
     5221 agcaccccgg tcgccgcggg gaccggcctg aaggtcctgg cctcgggctt ccagcccggc
     5281 gagcccctgc tgatcgccca ctgcacctcg aaggtcgagg aggacggcat cgtcggcacc
     5341 tgcgag
//
LOCUS       DATMFS010000597         4757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_251341, whole genome shotgun sequence.
ACCESSION   DATMFS010000597 DATMFS010000000
VERSION     DATMFS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251341"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..570)
                     /locus_tag="VNQ33_11425"
     CDS             complement(82..570)
                     /locus_tag="VNQ33_11425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWJ98762.1"
                     /translation="MKRWLVAGAVIEGAEGVLLVANRRHDGAVDWSTPGGVIDEGEDV
                     MAGLAREVHEETGLVVAGWDGLLYGIEVTAPDLGWHLRVEVHRAAAVEGDLTLADPDG
                     IVFDAGYVAVGECDERLAQSQRWVREPLSAWLGQRWTSARDFRYHVAGTDHASLTVSS
                     VP"
     gene            complement(638..1369)
                     /locus_tag="VNQ33_11430"
     CDS             complement(638..1369)
                     /locus_tag="VNQ33_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWJ98763.1"
                     /translation="MSALVIIPTYQEAENIVDVLGRVRDAAPEAHILVVDDGSPDGTA
                     DLAEGAAVDLGQIDVLRRASKSGLGPSYRAGFAWGLERDHTVLLEMDADLSHDPAVLP
                     SLIAAVTDGGADLAIGSRYIPGGAVPGWPTHRRLLSQWGNRYVGFMLRMPVRDATAGF
                     RAYTSSILEKVGLEQVRADGYGFQIEMAYEVSKAGGTIVEIPITFRDRIRGVSKMHPN
                     IVSEALLLVTRWGVRDRVRRLRRLR"
     gene            1460..1789
                     /locus_tag="VNQ33_11435"
     CDS             1460..1789
                     /locus_tag="VNQ33_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98764.1"
                     /translation="MPDAAPPPGHAPIPSALPSTGARAAAMVAIVVAGACGGLIGYKV
                     AQLSLTGEGDLIPGLIGLAGAAFAAGGVAVVSVLVLRAMGEWKTIVETGDPAAARKAQ
                     LDAKRRS"
     gene            1786..2586
                     /locus_tag="VNQ33_11440"
     CDS             1786..2586
                     /locus_tag="VNQ33_11440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWJ98765.1"
                     /translation="MTTDPAELLALAEPIATEVADHLRTSLAGDGPAVSSKSTPTDLV
                     TELDTWAEARITERILAARPDDAVSGEEGADVIGTSGVTWSVDPIDGTVNFVHGTPGF
                     CVSIAAQVDGRTVAGVVASPLHGEVFTATLGGGAFVDGAPIRCAQPASLARSVIATGF
                     GYDPQRRTRQAEVVAKVIGQIADIRRFGAAAVDLCWVACGRVDGYWEVGLNAWDHAAG
                     ALVAREAGAVVTGLTAGTSPGERFTLAAPPEVWADLAAVLAEAGAADV"
     gene            2655..3539
                     /locus_tag="VNQ33_11445"
     CDS             2655..3539
                     /locus_tag="VNQ33_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98766.1"
                     /translation="MRVRHQRFAIVAAVAAVCVLGTACKPLHETTPAGSGQVRYRDPV
                     FARVFKTADITYGSAPKIDGTNQTLKLDLYQPTGDKAAKRPAIVWVHGGGFKNGDKTS
                     GEIVDQADAFARRGFVNVSIDYRLSKGCAPFTDECIQGIEMAYHDAQAAVRFLRANAA
                     AYRIDTTRIAVAGSSAGGITAYNVAYGSEHPGSSGNPGPSSKVAAAVSLSGASLTTTV
                     NAGEPPTLDFHGDADGVVPLTWNDATLAEARKAKVIAERVVWKGDGHVPYGQHRTQII
                     IETRNFLYAAMDLGNAAK"
     gene            3618..4298
                     /locus_tag="VNQ33_11450"
     CDS             3618..4298
                     /locus_tag="VNQ33_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ98767.1"
                     /translation="MGTRILSVEDDERIRTAVRLALEDEGWTVDEAENGEEALVKFAQ
                     SPTDVVLIDIMLPDIDGFEVCRAIRRQSDVPIVMVTARDDTHDVVAGLEAGADDYLTK
                     PFAPKELSARIRALLRRARTADPASPHLRFGDLEVIPDQGVVRRNGQEVHLTKTEFKL
                     LVELATSPGKVFSRETLLDKVWGYDYFADGRLVDVHVRRLRTKVEADPANPRHVVTVR
                     GLGYKLQT"
     gene            4304..>4757
                     /locus_tag="VNQ33_11455"
     CDS             4304..>4757
                     /locus_tag="VNQ33_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98768.1"
                     /translation="MPASADPPVDPDDATQHRWWQGVRMPRLGLRARITLAFTLGALF
                     LSTILAGTTWALTRESILGQRESAVTRQAFRNSEVVQSQLSAGSEDDDLRKVLDSLDN
                     ETPSRAVVRDGYGRWTNQSVEFDERAIPTPLLRMAEQGTPGRMRVSIDG"
BASE COUNT          683 a         1819 c         1638 g          617 t
ORIGIN      
        1 agaacgcgtc catgtcgacg tggaggacgc ccgcatcggg ctcggggccg gcgccacccg
       61 gagaccgggg cgcaggcccg gtcacggtac cgacgagacc gtgaggctgg cgtggtcggt
      121 gccggcgacg tggtaccgga agtcgcgggc cgaggtccac cgctggccga gccaggccga
      181 cagcggctcg cgcacccacc gctgcgactg ggccaggcgc tcgtcgcact cgccgaccgc
      241 cacgtagccg gcgtcgaaga cgatgccgtc ggggtcggcc agggtgaggt cgccctccac
      301 cgcggccgcc cggtgcacct cgacccggag gtgccagccg aggtccggcg ccgtcacctc
      361 gatgccgtag agcaggccgt cccagccggc cacgaccagt cccgtctcct cgtggacctc
      421 gcgggcgagg ccggccatga cgtcctcgcc ctcgtcgatc acgccgcccg gggtggacca
      481 gtcgaccgcg ccgtcgtggc gtcggttcgc gaccagcaag acgccctcgg cgccctcgat
      541 cacggctccg gctaccaacc agcgcttcac agccgacagt ctgccagcgt ccgccggcgc
      601 gccgctcccc gccaggagca ccgggcccgg tccgccgtca gcggaggcgg cggagcctgc
      661 ggacccggtc ccggacaccc cagcgggtga cgagcaggag cgcctcgctg acgatgttcg
      721 ggtgcatctt ggacacgccc cggatgcggt cgcggaacgt gatcgggatc tcgacgatcg
      781 tcccgcccgc cttgctcacc tcgtaggcca tctcgatctg gaacccgtag ccgtcggcgc
      841 gcacctgctc gaggccgacc ttctccagga tcgaggaggt gtaggcccgg aagccggcgg
      901 tggcgtctcg cacgggcatg cggagcatga acccgacgta gcggttgccc cactgcgaca
      961 ggagccggcg gtgggtcggc cagcccggga cggcaccgcc cgggatgtag cgggagccga
     1021 tggcgaggtc ggccccgccg tcggtgaccg ccgcgatcag cgatggaagc accgccgggt
     1081 cgtgcgagag gtcggcgtcc atctccagca gcacggtgtg atcgcgctcg aggccccacg
     1141 cgaaccccgc ccggtacgac gggccgaggc ccgacttcga tgcccgccgc aagacgtcga
     1201 tctgacccag gtcgaccgcg gcgccctcgg cgaggtctgc ggtgccgtcg gggctgccgt
     1261 cgtcgaccac gaggatgtgc gcctcgggcg ctgcgtcacg gacgcgaccg agcacgtcga
     1321 cgatgttctc ggcttcctgg tacgtgggga tgatcacgag cgcagacatt cggagggtca
     1381 ctctatgagc acgccggacc ccagaccgga ccaatcggac ggatgggcca ccggacctac
     1441 cgctcagtag cttgggtcca tgcccgacgc cgcacctcca cccggccacg cgcccatccc
     1501 gtctgccctc ccctcgaccg gtgcgagggc cgccgccatg gtcgccatcg tcgtggcggg
     1561 ggcgtgcggc gggctcatcg gctacaaggt cgctcagctc tcgctgaccg gcgaaggcga
     1621 cctcatcccg gggctcatcg ggctggccgg cgccgcgttc gcggccggtg gcgtggccgt
     1681 cgtgtcggtc ctcgtgctgc gggccatggg cgagtggaag acgatcgtgg agaccggcga
     1741 tccggccgcg gcccgcaagg cccagctcga cgccaagcgc cgctcgtgac caccgacccc
     1801 gccgagctgc tggccctggc cgagccgatc gccaccgagg tcgccgacca cctgcgcacc
     1861 tcgctggccg gcgacggacc cgccgtcagc tccaagagca cgcccaccga cctggtcacc
     1921 gagctcgaca cctgggccga ggcccgcatc accgagcgca tcctcgccgc ccggcccgac
     1981 gacgccgtct cgggcgaaga gggggccgat gtcatcggca ccagcggggt gacctggtcg
     2041 gtcgacccca tcgacggcac ggtcaacttc gtccacggca cccccgggtt ctgcgtctcg
     2101 atcgcggcgc aggtcgacgg acgcaccgtc gccggcgtcg tagcgtcgcc cctgcacggc
     2161 gaggtgttca cggccacgct cggcggcggc gccttcgtcg acggcgcgcc catccgctgc
     2221 gcccagcccg cgtcgctggc gcgctcggtg atcgccaccg ggttcgggta cgacccgcag
     2281 cggcgcaccc gccaggccga ggtcgtggcc aaggtgatcg gccagatcgc cgacatccgc
     2341 cggttcggtg ccgccgccgt cgacctgtgc tgggtggcct gcggccgcgt cgacggctac
     2401 tgggaggtcg gcctcaacgc ctgggaccac gccgccggcg ccctcgtcgc ccgcgaggcc
     2461 ggtgcggtcg tcaccgggct cacggccggc accagcccgg gcgagcgctt caccctcgcc
     2521 gctcctccag aggtgtgggc cgacctcgcc gccgtgctgg ccgaagccgg cgccgccgac
     2581 gtctgacccc ctccgccgcc acccgcgggc tccgtcccgt acgatgcccc gaccgttctc
     2641 cagggggatc tgctgtgcga gtccgacacc aacgcttcgc catcgtcgcg gccgtcgccg
     2701 ccgtctgcgt gctgggcacg gcctgcaagc cgctgcacga gaccacgccg gcggggtccg
     2761 gccaggtccg ctaccgcgat ccggtgttcg cgcgcgtctt caagaccgcc gacatcacct
     2821 acggctccgc cccgaagatc gacggcacca accagacgct gaagctggac ctgtaccagc
     2881 ccaccggcga caaggcggcc aagcggccgg ccatcgtctg ggtgcacggc ggcggcttca
     2941 agaacggcga caagacctcc ggggagatcg tcgaccaggc cgacgcgttc gcgaggcggg
     3001 gcttcgtcaa cgtgtcgatc gactaccggc tgtccaaagg ctgcgcgccg ttcaccgacg
     3061 agtgcatcca gggcatcgag atggcgtacc acgacgccca ggccgcggtg cggttcctgc
     3121 gggccaacgc cgccgcctac cgcatcgaca cgacccgcat cgccgtggcc gggagctccg
     3181 ccggcggcat caccgcctac aacgtcgcct acggatccga gcaccccggg tcgagcggga
     3241 acccgggtcc gtcgtcgaag gtcgccgccg ccgtgtcgct gtcgggcgcc tcgctgacca
     3301 ccaccgtcaa cgccggcgag cccccgacgc tggacttcca cggcgacgcc gacggggtgg
     3361 tgccgctgac gtggaacgac gccaccctgg ccgaggcccg caaggccaag gtcatcgccg
     3421 agcgggtggt ctggaaggga gacgggcacg tcccctacgg ccagcaccgc acccagatca
     3481 tcatcgagac ccgcaacttc ctgtacgccg cgatggacct ggggaacgct gcgaagtagc
     3541 gcttcagcag gtcagacccc ggccctcccg ccctcgcgcc gcgcccggcg gggtccgctc
     3601 ccctacgatc accagccgtg ggcacccgga tcttgagcgt ggaagacgat gagcggatcc
     3661 gcacggcggt gcgcctggcc ctcgaggacg agggctggac ggtcgacgag gcggagaacg
     3721 gcgaggaggc gctggtgaag ttcgcccagt cgcccaccga cgtcgtcttg atcgacatca
     3781 tgctccccga catcgacggc ttcgaggtgt gccgggccat ccgccgccag tccgacgtcc
     3841 cgatcgtcat ggtcacggcc cgcgacgaca cccacgacgt cgtggccggg ctcgaggccg
     3901 gggccgacga ctacctcacc aagcccttcg ccccgaagga gctgtcggcc cgcatccgcg
     3961 ccctgctccg ccgggcccgc accgcggatc cggccagccc gcacctccgc ttcggcgacc
     4021 tcgaggtgat ccccgaccag ggtgtcgtgc gccgcaacgg ccaggaggtg cacctcacca
     4081 agaccgagtt caagctcctc gtggagctgg ccacctcacc ggggaaggtc ttcagccggg
     4141 agacgctgct cgacaaggtg tggggctacg actacttcgc cgacggccgc ctcgtcgacg
     4201 tccacgtccg ccggctgcgc accaaggtgg aggcggaccc ggccaacccc cgccacgtgg
     4261 tgacggtgcg cggcctgggc tacaagctcc agacctgaga cccgtgcccg cctccgcgga
     4321 tccaccggtc gatccggacg acgcgacgca gcaccggtgg tggcagggcg tgcgcatgcc
     4381 ccgcctgggg ctgcgggccc gcatcacgct cgcgttcacg ctgggcgccc tgttcttgtc
     4441 gaccatcctg gccggcacca cctgggccct gacccgcgag agcatcctcg gccagcgcga
     4501 gagcgccgtc acccggcagg ccttccggaa ctccgaggtc gtccagtccc agctgtctgc
     4561 cgggtcggaa gacgacgacc tgcggaaggt cctcgactcg ctcgacaacg agacgccgtc
     4621 gcgtgccgtg gtgcgcgacg gctacgggcg ctggacgaac cagagcgtcg agttcgacga
     4681 gcgcgccatc ccgaccccgc tcctccgcat ggccgagcag ggcacgccgg ggcggatgcg
     4741 cgtgtccatc gacggcg
//
LOCUS       DATMFS010000598         4259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_180762, whole genome shotgun sequence.
ACCESSION   DATMFS010000598 DATMFS010000000
VERSION     DATMFS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180762"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VNQ33_11460"
     CDS             <1..409
                     /locus_tag="VNQ33_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98769.1"
                     /translation="ATVGHLGARLVRAAGPKATKEDLAPYLRHLRSVDPSVVVLMAAA
                     MRAHDATDLLPDITTPTLVCAAGSDVFTPARCSEAIHHAVVGSELVTFADAGHTLPIE
                     EPEALAAVIGDFVVRRIAVKPRPRARTRPRRAS"
     gene            complement(462..2498)
                     /locus_tag="VNQ33_11465"
     CDS             complement(462..2498)
                     /locus_tag="VNQ33_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ98770.1"
                     /translation="MLRRRSNAPKVRYSRWDGTQVGFELDADAVLSEINDDLLYHGDL
                     NAALRRLMNSGFEDRNGERIAGMKDLMEKLRQSRRERLEQYDLGGVYDDIAQQLREVV
                     DMERASLDQLQQASADSGDQRRKEVTDDAVNERRMELDLLPPDLAGTVRELGEYDFTS
                     SEARERFEELTQQLREQLAQRWFDQMAGAMSDVSPEALARTKDMLAELNTMLEDRAAG
                     REPDFDGFMERYGDFFPEGPRDLDELLELMAQRMAAMQAMLNSMTPEQRAQLQGLSEQ
                     LLEDMDLQWQMDQLSANLQQAFPDMGWNRRYDFEGQDPLGFADAAQVMNELGDLDQLE
                     QLMRGAASPGALAEVDLERARQLIGDEAAESLEKMAELAKMLTDAGLIENREGRYELT
                     PAGLRRVGKHALNDLFSKLARDKLGQHELTRTGLGHERSMDTKPYEFGDPFNLHIERT
                     IRNAVSRTGGGTPVTLSPDDFEIEQTELSVRSATVLMVDLSLSMPMRDNFLAAKKVAM
                     ALHSLISSRYPRDYLGLVGFSEVARIIEPQELPEVSWDYVYGTNMQHGFQLARKMLSR
                     EHGTKQIIMITDGEPTAHITASGHPVFHYPPTRETVDATLTEVMRATREGIRINTFML
                     DATAHLQQFIEQLTQLNRGRAFFTTPETLGDYVLVDFLESRRAMSRAGRSRRAG"
     gene            2556..3695
                     /locus_tag="VNQ33_11470"
     CDS             2556..3695
                     /locus_tag="VNQ33_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98771.1"
                     /translation="MADAEDLLWGSRCPVASGAGPASDLAARRQAHGGAPAEALDLAR
                     DLDALEVRAGLGHDSVRLTFDWARLEPEAGRRDRAVVDHLREVLTTARRLGLQAWGCL
                     HERALPGWFSVDEHGLADDRSRRYYWARHVEQVGGELGDLVDGWLPMVEPSRWALQGW
                     LTAVGPPGHLDDAEGFAGALEGALLGSVEAARRLRGSGRPVATGQWIVPAFAARVTPD
                     VPPSPDAEAMTSTVDEALFGCWRRLLAEETLVVGHRAPISVPGARTAFDRIGFTYRHG
                     IAVRGDGALLPYPQQLPIRTDGQVTWAEGLALALHHVAETFGDRPLHLVGYGVAADDP
                     AFDEFEHETGAIIRGAVSDGIDLRGAWWEPDLDVDRPPRRPLAAG"
     gene            3824..>4259
                     /locus_tag="VNQ33_11475"
     CDS             3824..>4259
                     /locus_tag="VNQ33_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98772.1"
                     /translation="MRLPDRIWVPAAGGLALALLFAGALLVRAEGDPSLLVHAGAPCT
                     TVDALPALSSCDASVADIDEAAIGALGQGGARGSLTVQDADQAFDGQFFYRLGVSPWS
                     TDDRVAGVQDDLPSLRNARWGYGALAWAVSGGDPDLVPWALIA"
BASE COUNT          512 a         1537 c         1561 g          649 t
ORIGIN      
        1 cgccaccgtg ggccacctcg gcgcccgctt ggtccgtgcc gcggggccca aggccaccaa
       61 ggaggacctg gccccgtacc tccgccacct gcgctcggtc gacccgtccg tcgtcgtgtt
      121 gatggccgcc gcgatgcgcg cccacgacgc caccgacctc ctccccgaca tcaccacgcc
      181 gacgctcgtc tgcgccgcgg gctccgatgt gttcacgcca gcgcggtgct ccgaggcgat
      241 ccaccacgcg gtcgtcggca gcgagctggt gacgttcgcc gacgcgggcc acaccctccc
      301 gatcgaggaa cccgaggcgc tggctgcggt catcggggac ttcgtggtgc gccggatcgc
      361 cgtcaagccc cgcccccgag cgcgaacgcg gccgcgccgc gcgtcgtagc accgcacctc
      421 gggccgggcc gggtggaccg cggagcggcc agcccggctc gtcagccggc gcggcggctc
      481 cggccggcgc ggctcatggc ccggcgggac tcgaggaagt cgaccaggac gtagtcgccc
      541 agggtctccg gggtcgtgaa gaaggctcga ccgcggttca gctgggtgag ctgctcgatg
      601 aactgctgga ggtgggcggt ggcatcgagc atgaacgtgt tgatgcggat gccctcgcgg
      661 gtggcgcgca tcacctcggt caaggtggcg tcgacggtct cgcgcgtcgg cgggtagtgg
      721 aagaccgggt ggccgctcgc cgtgatgtgg gcggtgggct ccccgtcggt gatcatgatg
      781 atctgcttgg tgccgtgctc ccgggagagc atcttgcgag ccagctggaa gccgtgctgc
      841 atgttggtgc cgtagacgta gtcccagctg acctcgggga gctcctgcgg ctcgatgatc
      901 cgggcgacct cgctgaagcc gaccagcccg aggtagtccc gcgggtaccg gctggagatg
      961 agcgagtgga gcgccatcgc caccttcttg gcggcgagga agttgtcgcg catcggcatc
     1021 gacagcgaca gatcgaccat cagcacggtg gcggagcgga ccgacagctc ggtctgctcg
     1081 atctcgaagt cgtccggtga cagcgtgacc ggcgtgccgc cgccggtgcg ggagacggcg
     1141 ttgcggatgg tgcgctcgat gtggaggttg aacgggtcgc cgaactcgta gggcttggtg
     1201 tccatcgacc gctcgtgacc gaggcccgtc ctggtcagct cgtgctggcc gagcttgtcg
     1261 cgcgccagct tggagaagag gtcgttgagg gcgtgcttgc cgacgcggcg gaggccggcg
     1321 ggcgtgagct cgtagcggcc ctctcggttc tcgatgaggc cggcatcggt gagcatcttg
     1381 gccagctcgg ccatcttctc gaggctctcg gccgcctcgt cgccgatgag ctggcgagcc
     1441 cgctccagat cgacctcagc cagcgctccg gggctggcgg cgccccgcat gagctgctcg
     1501 agctggtcga ggtcgccgag ctcgttcatg acctgggcgg catcggcgaa cccgaggggg
     1561 tcctgaccct cgaagtcgta ccgccggttc cagcccatgt cggggaaggc ctgctggagg
     1621 ttggccgaca gctggtccat ctgccactgg aggtccatgt cctcgagcag ctgctcggac
     1681 aggccctgga gctgcgcccg ctgctcgggc gtcatcgagt tgagcatcgc ctgcatggcc
     1741 gccatccgct gggccatcag ctccagcagc tcgtcgaggt ctcggggccc ctcggggaag
     1801 aagtcgccgt agcgctccat gaagccgtcg aagtccggct cgcgccccgc agcgcggtcc
     1861 tcgagcatcg tgttgagctc ggcgagcatg tccttggtgc gcgccagcgc ctcgggcgag
     1921 acgtcgctca tggcgccggc catctggtcg aaccaccgct gggccagctg ctcgcgcagc
     1981 tgctgggtca gctcctcgaa gcgctcgcgc gcctccgaac tggtgaagtc gtactcgccg
     2041 agctcgcgga ccgtgccggc gaggtcgggc gggagcaggt ccagctccat gcgccgctcg
     2101 ttcacggcgt cgtcggtgac ctccttgcgg cgctggtcgc cggagtcggc cgacgcctgc
     2161 tggagctggt cgagcgaggc ccgctccatg tcgacgacct cgcgcagctg ctgggcgatg
     2221 tcgtcgtaga cgccgccgag gtcgtactgc tcgagccgct cgcgccggct ctggcgcagc
     2281 ttctccatca ggtccttcat gcccgcgatg cgctcgccgt tgcggtcctc gaagcccgag
     2341 ttcatcagcc ggcggagcgc ggcgttgagg tcgccgtggt acagcaggtc gtcgttgatc
     2401 tcgctcagca cggcgtcggc gtcgagctcg aagccgacct gggtgccgtc ccagcgcgag
     2461 taccggacct tcggagcgtt cgagcggcgg cgcagcacgg ggtcgaggct acgcccgctt
     2521 cgcgcaaacc gacccgggtg ggcgaggatg cctgcatggc cgatgccgag gacctcctgt
     2581 ggggatcgcg ctgccccgtc gcgagcggcg ccgggccggc gtcggacctc gccgcccggc
     2641 gccaggccca cggcggcgcg cccgccgagg cgctggatct ggcccgcgac ctcgacgcgc
     2701 tcgaggtgcg agcggggctc ggtcacgact cggtgcgcct cacgttcgac tgggctcggc
     2761 tggagccgga ggccggccgc cgtgaccgcg ccgtggtcga tcacctgcgc gaggtgctca
     2821 cgaccgcccg ccggctcggc ctccaggcgt ggggctgcct ccacgagcga gccctcccgg
     2881 gctggttctc ggtcgacgag cacggcctcg ccgacgaccg gtcgcgccgc tactactggg
     2941 cgcgccacgt cgagcaggtc ggcggtgagc tgggcgacct ggtcgacggc tggctcccga
     3001 tggtcgagcc gagccggtgg gccctccagg gctggctcac cgcggtgggt ccgcccggtc
     3061 acctcgacga cgccgagggc ttcgccggcg ccctcgaggg cgcgctgctc gggtcggtcg
     3121 aggcggcgcg gcgcctgcgc gggtccggtc gtccggtggc gacgggccag tggatcgtgc
     3181 ccgccttcgc cgcccgggtc acgcccgacg ttccaccgtc gcccgacgcc gaagccatga
     3241 ccagcaccgt cgacgaggcg ctgttcgggt gctggcgacg actcctggcg gaggagacgc
     3301 tggtcgtcgg ccaccgcgcc ccgatctcgg tgcccggcgc ccggaccgcg ttcgaccgca
     3361 tcggcttcac gtaccgccat gggatcgcgg tccgcggcga cggcgccctg ctcccctacc
     3421 cccagcagct gcccatccgg acggacggcc aggtgacgtg ggccgaaggc ctggcgctcg
     3481 cgctccacca cgtggccgag acgttcggcg accggccgct gcacctggtg ggctacggcg
     3541 tcgcggccga cgatccggcg ttcgacgagt tcgagcacga gaccggcgcg atcatccgag
     3601 gtgccgtcag cgacggcatc gacctccggg gcgcctggtg ggagcccgac ctcgacgtcg
     3661 accggccgcc ccggcgcccc cttgcggccg gctgactcca cccgcgccgc acctcccgcc
     3721 gcccgctgct gctcatccgc ggcgtgcgcg tcgaatcgac gtcgattcga cgcgcacggg
     3781 ccgcgtcgcc gaccggccga gcccggcgcg tagcgtcgtc gcggtgaggc tgcccgaccg
     3841 catctgggtt cccgcggccg gcggactggc cctggcgctc ctgttcgccg gcgcgctgct
     3901 ggtgcgggcc gagggggatc cgtcgctgct cgtccacgcc ggcgcgccgt gcaccaccgt
     3961 cgacgcactg cccgcgctct cctcgtgcga cgcgagcgtg gccgacatcg acgaggcggc
     4021 catcggcgcc ctaggacagg gcggcgcgcg gggttcactg accgtccagg acgcggacca
     4081 ggcgttcgac gggcagttct tctaccggct cggggtctcc ccctggtcca ccgatgaccg
     4141 ggtggcaggt gtccaagacg acctgccgtc gctgcgcaac gcccgctggg ggtacggcgc
     4201 cctcgcctgg gccgtcagcg gcggcgaccc cgacctggtg ccctgggcgc tcatcgccg
//
LOCUS       DATMFS010000599         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_160518, whole genome shotgun sequence.
ACCESSION   DATMFS010000599 DATMFS010000000
VERSION     DATMFS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160518"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VNQ33_11480"
     CDS             <1..266
                     /locus_tag="VNQ33_11480"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HWJ98773.1"
                     /translation="VCETLAAGLPLVVAPIRDDQPVVADQVIRAGAAVRVKFARVRAD
                     ALQAAVGQALTDPGLLAAAERIQASFAAAGGPAAAADALVALA"
     gene            281..1048
                     /locus_tag="VNQ33_11485"
     CDS             281..1048
                     /locus_tag="VNQ33_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98774.1"
                     /translation="MERVVANGVGLAYQRAGRGSRLLFLNGSGMTMTTSALLRKPFVA
                     SFDLLTLDHRGMGASSVPDGPYGMADCAADALAVADAVGWDRCSVVGISFGGMVAQEL
                     AVTAPERVERLALLCTSAGGAGGSSYPLHEQPPTPTVLDTRFTDEWLAEHPRDAALVD
                     FLAKRTLVDKDDDTIRGERLQLEARSHHDVWGRLDRITAPTIVAGGRFDGLAPFTNSE
                     AIASRIAGAELHGYDGGHAFFVQDPQAFPDVVAFLQR"
     gene            complement(1088..1588)
                     /locus_tag="VNQ33_11490"
     CDS             complement(1088..1588)
                     /locus_tag="VNQ33_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWJ98775.1"
                     /translation="MTRPARIEPGPGQESVWDYPRPPRIDPITSHIRVVHAGQTVVDT
                     AKAIRILETSQPPSIYVPPSDADLTVLHPSDRHTFCEWKGTASYLDLVVDGAPPVADA
                     AWTYPSPDPRYEAITGYLAFYPQRVDACFVDGEQADPGEGSFSGGWITSAVVGPFKGG
                     PGTAGW"
     gene            complement(1599..1922)
                     /locus_tag="VNQ33_11495"
     CDS             complement(1599..1922)
                     /locus_tag="VNQ33_11495"
                     /inference="COORDINATES: protein motif:HMM:NF039377.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6204 family protein"
                     /protein_id="HWJ98776.1"
                     /translation="MSVSVHRVVVRGHFADLTPEQQAALVAEADQHSFHSVAYTEWGT
                     FIYEPNLVAFNFRYEVRIDDEADPKPDPVEVGLAKAQASLAEWGLGSKHLRASATDMS
                     QVWSG"
     gene            2059..3825
                     /locus_tag="VNQ33_11500"
     CDS             2059..3825
                     /locus_tag="VNQ33_11500"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWJ98777.1"
                     /translation="MGDVPVTERRPGCRFTLFTMPLPRSLFQRDAPADHDLDVRSGTW
                     PEGLTGELVLSAPHPDTFDGPHPFFGEGMTYRLSLEPGRHGAGPASLAWRQAKIDSPS
                     ARLRAARPDVFTPTMIGVQSPFGHLNAANTAPLPWGDRLFMAWDAGRPTEIDPLTLQF
                     LGDVGHRSEWLDFEVGPQPVLPMVMSTAHPVIDPDRNVLWTVNTHWGSLHVVRWDGQG
                     PVQRWPVAHALIPQSVHTITQTREWLIVADCAYKVEPQVLSGGARTEPANRSGPVYLI
                     RKADLEAVPPGTEVAAQVFEMAPENNHYYATYDDTDGVAILFEHTESADIAMTQQVGD
                     LDALGRPCDPALRGLYGFPMAPDRTTFVIVDPVTGTILHQAEQRDPERLWTRQLNAID
                     WSTEGRSNPTLHHTVHQGFRPEGITQAMLALYGDRVDRTLLPDGETLPRVISTALPDL
                     QITAEWELAQDDFPTSPIFVPRDPGADPSKSRCSGSDPGGHDGWVVVPIINDAGFRVE
                     VFSAGDVGAGPVAVAAAAGMTVPFVLHSAWMPRVVSTAGDGRHRNRFAAELDRLDELP
                     DDLAAVALQVADDLAQGAPLPA"
BASE COUNT          519 a         1444 c         1407 g          580 t
ORIGIN      
        1 ccgtgtgcga gacgctcgcc gccgggctgc ccctggtggt ggcgccgatc cgcgacgacc
       61 agcccgtggt cgccgaccag gtgatccgtg ccggcgccgc cgtccgggtg aagttcgccc
      121 gggtgcgcgc cgacgcgctg caggccgcgg tcggccaggc gctcaccgac cccggcctcc
      181 tcgctgcggc cgagcgcatc caggcctcgt tcgccgccgc tggtggtccg gctgcggcgg
      241 ctgacgcgct cgtcgccctc gcctgacaag ctgaccgccg atggagcggg tggtggcgaa
      301 cggcgtgggg ctggcgtacc agcgggcagg ccgtggctcg cggctgctgt tcttgaacgg
      361 gtcgggcatg acgatgacga catcggccct gctccgcaag cccttcgtgg cgtcgttcga
      421 cctgctcacc ctcgaccacc gggggatggg cgccagcagc gtccccgatg gtccctacgg
      481 gatggctgac tgcgccgcgg acgcgctcgc cgtggccgat gccgtggggt gggaccggtg
      541 ctcggtcgtg ggcatcagct tcggcggcat ggtggcccag gaactggcgg tcaccgcacc
      601 cgagcgggtc gagcggctgg cgctgctgtg cacgtcggcg ggtggtgcgg gtgggtcctc
      661 gtacccgctg cacgagcagc ctccgacgcc gacggtcctc gacacccgct tcacggacga
      721 gtggttggcg gagcaccccc gcgacgccgc gctggtcgac ttcttggcca agcggacgct
      781 ggtcgacaag gacgacgaca cgatccgcgg tgagcggttg cagctcgagg cgcgcagcca
      841 ccacgacgtg tggggccggc tggaccgcat caccgccccg acgatcgtgg ccggcgggcg
      901 gtttgacggg ctggccccgt tcacgaacag cgaggccatc gcctctcgca ttgcgggcgc
      961 cgagttgcac ggttacgacg gcggccacgc gttcttcgtg caggacccgc aggcgttccc
     1021 cgacgtcgtc gccttcctcc agcgctgagc ggtcgggggc cgagcggacc ccgccggccg
     1081 aggcgggcta ccagccggcg gtgccggggc cgcccttgaa cgggccgacc accgcggacg
     1141 tgatccaccc gccggagaag ctgccctcgc ccgggtcggc ctgctcgccg tcgacgaagc
     1201 aggcgtcgac gcgctgcggg tagaaggcga ggtagccggt gatggcttcg tagcgcgggt
     1261 ccgggctcgg gtacgtccag gcggcgtcgg ccaccggcgg tgcgccgtcc accacgaggt
     1321 ccaggtagga cgccgtgccc ttccactcgc agaacgtgtg ccgatccgac gggtggagca
     1381 cggtgaggtc ggcgtcgctg ggcggcacgt agatgctggg tggctggctg gtttcgagga
     1441 tgcggatcgc cttcgcggtg tcgacgacgg tctggccggc gtggacgacc cggatgtggg
     1501 atgtgatcgg gtcgatgcgc ggcgggcgcg ggtagtccca caccgactcc tggccggggc
     1561 cgggctcgat gcgtgccggc ctggtcatgc gggtgctcct agccggacca cacctgcgac
     1621 atgtccgtgg ccgatgcccg caggtgcttg gacccgaggc cccactcggc gagcgaggcc
     1681 tgcgccttgg ccaacccgac ctcgaccggg tccggtttcg ggtccgcttc gtcgtcgatc
     1741 cggacctcgt agcggaagtt gaaggccacg aggttcggct cgtagatgaa cgtgccccac
     1801 tcggtgtagg ccacggagtg gaacgagtgc tggtcggctt cggcgaccag cgcggcctgc
     1861 tgctccggcg tgaggtcggc gaagtggccg cgcacgacga cgcggtggac ggagacggac
     1921 atcgacccag tctggctcgg gctcgagccg tctccgccac ggatcgccgg cgacggcacc
     1981 cgatcgcatc ctggtcactg ccacgtggtc ccccctggcc ccttcgtgcc gccgcaccct
     2041 acgcaggatc ggcggctgat gggtgacgtg cctgtcacgg agcggcgccc ggggtgcagg
     2101 tttacactgt tcaccatgcc gctgccccgg tcgctgttcc agcgagatgc gcccgccgac
     2161 cacgacctcg acgtgcgatc cgggacctgg cccgagggcc tcaccggcga gctggtgctg
     2221 agcgcgccgc accccgacac gttcgacggg ccccatccgt tcttcggcga gggcatgacc
     2281 taccgcctgt cgctcgagcc cggccgccac ggtgccggac cggcatcgct ggcgtggcgc
     2341 caggccaaga tcgactcgcc ctcggcccgc ctccgtgcgg cccggcccga cgtcttcacg
     2401 ccgacgatga tcggcgtgca gtccccgttc ggccacctca acgccgcgaa cacggcaccg
     2461 ctgccgtggg gcgaccggct gttcatggcg tgggacgccg gccgcccgac cgagatcgac
     2521 ccgctcacgc tgcagttcct cggcgacgtc ggccaccggt ccgagtggct cgacttcgag
     2581 gtcggcccgc agccggtcct cccgatggtg atgagcaccg cgcacccggt catcgacccc
     2641 gaccgcaacg tgctgtggac cgtcaacacc cactggggct cgctccacgt ggtgcggtgg
     2701 gacggccagg gaccggtgca gcgctggccc gtcgcccatg cgctgatccc gcagtcggtc
     2761 cacaccatca cccagacccg cgagtggctg atcgtggccg actgcgcgta caaggtggag
     2821 ccccaggtgc tctcgggcgg cgctcgcacc gagccggcca accggtccgg gcccgtgtac
     2881 ctcatccgca aggccgacct cgaggcggtg ccgccgggca ccgaggtggc ggcgcaggtg
     2941 ttcgagatgg cgccggagaa caaccactac tacgccacct acgacgacac ggacggcgtc
     3001 gcgatcttgt tcgagcacac cgagagcgcc gacatcgcca tgacccagca agtgggcgac
     3061 ctcgatgcgc tcggccgtcc gtgcgacccg gcgctgcgcg gcctctacgg gttcccgatg
     3121 gcgccggacc ggaccacgtt cgtgatcgtc gacccggtca ccggcacgat cctgcaccag
     3181 gccgagcagc gcgaccccga gcggctctgg acccgccagc tcaacgccat cgactggagc
     3241 acggaggggc ggtcgaaccc gacgctccac cacacggtcc accaggggtt ccgacccgag
     3301 ggcatcaccc aggcgatgct cgccctctac ggcgaccgcg tcgaccgcac gctcctgccc
     3361 gacggcgaga ccctcccgcg cgtgatcagc acggcgctcc ccgatctgca gatcacggcc
     3421 gagtgggaac tggcccagga cgacttcccg acgtcgccga tcttcgtgcc ccgcgacccc
     3481 ggcgccgatc cctcgaagag ccgctgctcg ggctcggacc ccggtggcca cgacgggtgg
     3541 gtcgtggtcc cgatcatcaa cgacgccggc ttccgggtcg aggtgttctc cgccggtgac
     3601 gtgggcgctg gtcccgtggc ggtcgccgct gctgcgggca tgaccgtgcc cttcgtgctg
     3661 cactcggcgt ggatgccgcg ggtggtgtcg acggcgggcg acggccgcca ccgcaaccgc
     3721 ttcgccgccg agctcgaccg gctggacgag ctccccgacg acctcgccgc cgtggccctc
     3781 caggtcgccg acgacctcgc ccaaggcgcc cccctgcctg cttgagcagg ccgctcgctt
     3841 ctcggcacgc cggcagcagc ggcgctcgcc ggaagttggt gtgggaacct ctgcctgaca
     3901 gcagttcgca caccaacttc gatcgggttg cagggtcgtt ggattgcacc
//
LOCUS       DATMFS010000600         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_323843, whole genome shotgun sequence.
ACCESSION   DATMFS010000600 DATMFS010000000
VERSION     DATMFS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323843"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VNQ33_11505"
     CDS             <1..411
                     /locus_tag="VNQ33_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98778.1"
                     /translation="PSGRNYWSMKLITITRPEMLARTTGSSEFYRKAGGTIPTFVDAA
                     YQAALGRASDPLGRAFWIKQLQNGRHVESVARSLVKSNEYRRTETKNTYQRVLDRQPT
                     DAERDAWITPADPRIEILIARLAASAEFYDVTIP"
     gene            408..1430
                     /locus_tag="VNQ33_11510"
     CDS             408..1430
                     /locus_tag="VNQ33_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98779.1"
                     /translation="MTLLVPRRTGGGGRRRPLAAAAVLVALAATSLAPARPAAAAPDR
                     VAVTGGCRAVGTSDAPSGGGPDRATVVVDTGSGAVWSACISFSGAISGVEALERAQSV
                     ITDLDPVYDQYSGLGKAVCKLRGVGTSPPDCLGKSVNSWWLSLNGQVTGKGASSITVK
                     DGDVEGWRYGTGGTPRAATAGTEAAAAPPAPPTTKPPVAPTTQPPAAPTTKPGGSGSM
                     TGSTKPDGTPADPTTTRPGDTTTTEAGQTTTTRAETTTTSKGEESDAGEPASSGGSDG
                     GSGGSSSESASGAAASGTSADGDGGSGGSSLPAVLGFLVVIAAIGGGAVLIRRRRLAG
                     PATPAA"
     gene            complement(1518..2240)
                     /locus_tag="VNQ33_11515"
     CDS             complement(1518..2240)
                     /locus_tag="VNQ33_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWJ98780.1"
                     /translation="MSTDDAPDLSGLRALFLNATLKRSPEVSNTQALVDASAAIMEAH
                     DVATTSIRVIDHDVATGVWPDMTEHGWETDEWPAIQEQVWAADILVLAGPIWLGDNSS
                     VMKQIIERLYGSSHLLNSAGQYRYYGRTAGCLITGNEDGIKHCAQNVLYSLQHIGYVI
                     PPQADAGWIGEAGPGPSYLDPGSGGPDNDFTNRNTTFMTWNLLHLAKLLKDAGGIAAH
                     GNQRAAWDDGERFDFENPEYRR"
BASE COUNT          312 a          848 c          803 g          322 t
ORIGIN      
        1 ccctccggac ggaactactg gtcgatgaag ctgatcacca tcacccgccc ggagatgctg
       61 gcccgcacca ccggctcgtc ggagttctac cggaaggccg gcggcacgat cccgacgttc
      121 gtggacgccg cgtaccaggc cgcgctcggc cgagcgtctg acccgttggg ccgcgccttc
      181 tggatcaagc agctccagaa cggccgccac gtcgagtccg tcgcccgcag cctggtgaag
      241 agcaacgagt accgccggac cgagaccaag aacacctacc agcgggtgct cgaccgtcag
      301 cccaccgacg cggaacgaga tgcgtggatc accccggccg acccgcgcat cgagatcctg
      361 atcgcccggc tcgccgcgtc cgccgagttc tacgacgtga ccatcccgtg accctgctcg
      421 tcccccgtcg caccggcggc gggggacgcc ggcggccgct cgcggccgca gcggtgctcg
      481 tggccctggc cgccacgagc ctggcgcctg cccgcccggc cgccgccgcg cccgatcgcg
      541 tcgcggtcac cggcggctgc cgcgccgtcg gcacctctga cgcgccgagc ggcgggggac
      601 cggatcgggc caccgtcgtg gtcgacaccg gctcgggtgc ggtctggtcg gcgtgcatct
      661 cgttcagcgg cgccatctcg ggcgtcgagg cgctcgagcg cgcccagtcg gtgatcaccg
      721 acctcgaccc cgtgtacgac cagtactcgg gcctgggcaa ggcggtctgc aagctccgcg
      781 gcgtcggcac gtcgccgcct gactgcctgg gcaagtcggt gaactcctgg tggctgagcc
      841 tcaacggcca ggtcaccggc aagggcgcca gctcgatcac cgtgaaagac ggtgatgtcg
      901 aaggctggcg ctacggcacc ggcggtacgc ctcgagcggc cactgccggc accgaggctg
      961 ccgctgcccc gccggcgccg ccgaccacca agccgccggt cgcgccgacc acgcagcccc
     1021 cggcggcgcc caccaccaag cccggcggga gcgggtcgat gaccgggtcc acgaagcccg
     1081 acggcacgcc cgccgacccg acgaccaccc gtcccggcga caccaccacg accgaagccg
     1141 gccagaccac caccaccagg gccgagacga ccaccaccag caagggcgag gagagcgacg
     1201 ctggtgagcc ggcctcctcc ggcggctccg atggcggctc gggcggcagc agcagcgaat
     1261 cggcctccgg agccgcggcg tcgggcacct cggcggacgg cgacggcggc tcgggtggca
     1321 gctccctgcc ggcggtgctc ggcttcctcg tcgtcatcgc cgccatcggc ggcggggccg
     1381 tcctcatccg ccggcgccgc ctcgccggcc ctgcgacccc cgccgcttga gccggaccgt
     1441 cgcccgctgc gtccgagctt cgtcgctcag cgcctgagac cggaacgcag acctgcggag
     1501 tcggccgggg ctggtcctca gcggcggtac tcggggttct cgaagtcgaa gcgctcgccg
     1561 tcgtcccagg cggcgcgctg gttgccgtgg gcggcgatgc cgccggcatc tttcagcagc
     1621 ttggcgaggt gcagcaggtt ccacgtcatg aacgtggtgt tccggttggt gaagtcgttg
     1681 tccggtccgc ccgagccggg gtccaggtac gaggggcccg ggccggcctc gccgatccac
     1741 ccggcatccg cctgcggcgg gatgacgtag ccgatgtgct gcagcgagta gagcacgttc
     1801 tgggcgcagt gcttgatgcc gtcctcgttg ccggtgatga ggcagccggc ggtgcggccg
     1861 tagtagcggt attgcccggc gctgttcagc aggtgggagc tgccgtagag ccgctcgatg
     1921 atctgcttca tgaccgagct gttgtcgccg agccagatcg gcccggccaa gacgaggatg
     1981 tcggcggccc agacctgctc ttggatggcc ggccactcgt cggtctccca cccgtgctcg
     2041 gtcatgtccg gccacacgcc ggtggcgacg tcgtggtcga tcacgcggat gctcgtggtg
     2101 gcgacgtcgt gcgcctccat gatcgccgcg ctcgcgtcga ccagcgcctg ggtgttcgac
     2161 acctcgggcg accgcttcag ggtggcgttg aggaacaggg cccgcagccc gctgaggtcc
     2221 ggtgcatcgt cggtgctcat gtggacagcc ttccagcgat cgaccggtca tgctcaggga
     2281 tccgg
//
LOCUS       DATMFS010000601         2976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_137107, whole genome shotgun sequence.
ACCESSION   DATMFS010000601 DATMFS010000000
VERSION     DATMFS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137107"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VNQ33_11520"
     CDS             complement(<1..196)
                     /locus_tag="VNQ33_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98781.1"
                     /translation="MSDTLEPLVWGEIDGQTITFPMEVTDFDAATFVFSVPADAAQAL
                     LPGADFSVIAVDGVAQLVVAL"
     gene            342..1589
                     /locus_tag="VNQ33_11525"
     CDS             342..1589
                     /locus_tag="VNQ33_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ98782.1"
                     /translation="MTDLQQPTRDWAVDFDHTSEDYAQSATEIWDDLRRRCPVAHGEA
                     HGGVWMPTRHTDISAIANDTEHFSSEGVIVSIFKPEGLAPMGYAPPITADPPFHKDSR
                     RILLPAFAPRAIDKLEEATRATCRELLDELLAAGTPVVDAAVQYSQNIPVRVIAHLLG
                     VPPEDGDRFRLFIHRIIEKPGTYSGTVAPEDTLVYYIEQTLQRRRQEPPRDDLIGYLL
                     SLEVDGEPLPDAQIFGTIGLLIIAGIDTTWSAIGASLWHLAKNPADRQRLAEDPAVLP
                     FAVEEFLRFYAPVTMARIAASDTEIGGCPVSERDWVLLPFPAANRDPEAFERADEFII
                     DRQRNRHVAFGLGIHRCAGSNLARMELTVAIDEWMKRIPDFELADDDPDAVRWSTGQV
                     RGPRELPVRILRTNAPETAEAAG"
     gene            1589..1783
                     /locus_tag="VNQ33_11530"
     CDS             1589..1783
                     /locus_tag="VNQ33_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWJ98783.1"
                     /translation="MRIAFDREKCQGHGRCYALAPDLFDADDEGYAVVLEAGDLTEAQ
                     LADAQLAADNCPEYAISIDV"
     gene            complement(1802..2236)
                     /locus_tag="VNQ33_11535"
     CDS             complement(1802..2236)
                     /locus_tag="VNQ33_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWJ98784.1"
                     /translation="MGGGRGDDAAGCAPERPTLRFVRAPSLVDHALHALVVLAAAPQG
                     TSLKIRELCAPQGLSRKFVAAALNDLRLAEVVGSRRGSNGGYWLARPAHEITVADVFD
                     AVDVRTPTEAPGFDGTLDVWVRAERAVRSGFSAVTIADLASR"
     gene            complement(2238..2750)
                     /locus_tag="VNQ33_11540"
     CDS             complement(2238..2750)
                     /locus_tag="VNQ33_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWJ98785.1"
                     /translation="MAGRLVVNGSPSHPSRSRTLAVHVLELLGGGDVLDLVDLDPAGL
                     LALRSSPDVDAAVRRVAAASILVVATPVYRATYTALTKTVFDLQPRAALAGTVVIPVA
                     TGDAAEHALAIDHGLRPLIASLGGWTTPTGIYATRADFGDDGQASAGIHASAAAAAAE
                     ALALAGALGG"
     gene            complement(2750..>2976)
                     /locus_tag="VNQ33_11545"
     CDS             complement(2750..>2976)
                     /locus_tag="VNQ33_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98786.1"
                     /translation="LVGSHDEVAERLAEYHEIGFDHVILSGQPHLEEAYWFGEGVIPK
                     LKAAGIAAEPGIPVPTADPAAAEPALAAAR"
BASE COUNT          446 a         1137 c         1021 g          372 t
ORIGIN      
        1 acagggcgac cacgagctgg gccacgccgt cgaccgcgat caccgagaag tcggcgcccg
       61 gcagcagggc ctgggccgcg tcggccggga ccgagaacac gaaggtggcg gcatcgaagt
      121 cggtcacctc catggggaag gtgatggtct ggccgtcgat ctcgccccag accaggggct
      181 ccagggtgtc gctcatcggg ttcctcctcg gcgttgggac caccagtaga gcacccggca
      241 cgcggtcgtc ggtcacacac cccgcccgag gcctcgataa cgcgtattat cgaggtgatc
      301 gagcggggga gacgtccttc caccgcccca ggggaaccac catgaccgac ctgcagcaac
      361 ccacccgcga ctgggccgtg gacttcgacc acacgtccga ggactacgcc cagtccgcca
      421 ccgagatctg ggacgacctc cgccgccgct gcccggtcgc ccacggcgag gcccacggcg
      481 gcgtctggat gcccacccgc cacaccgaca tcagcgccat cgccaacgac accgagcact
      541 tcagctccga gggcgtgatc gtcagcatct tcaagcccga gggcctggcc ccgatgggct
      601 acgcgccgcc catcaccgcc gacccgccgt tccacaagga ctcccggcgc atcctgctgc
      661 cggcgttcgc gccccgggcc atcgacaagc tcgaggaggc gacccgcgcc acctgccgcg
      721 agctgctcga cgagctgctc gccgccggca cgcccgtcgt cgacgccgcc gtccagtact
      781 cgcagaacat cccggtccgg gtcatcgccc acctgctcgg cgtgcccccc gaagacggcg
      841 accgcttccg cctcttcatc caccgcatca tcgagaagcc cggtacctac agcggcaccg
      901 tcgctcccga ggacacgctc gtctactaca tcgagcagac cctccagcgc cgccgccagg
      961 agccgccgag ggacgacctc atcggctacc tcctgtcgct cgaggtcgat ggcgagcccc
     1021 tgcccgacgc ccagatcttc ggcacgatcg ggctgctgat catcgccggc atcgacacca
     1081 cctggtccgc catcggcgcc agcctgtggc acctggccaa gaacccggcc gaccggcagc
     1141 gcctggcgga ggacccggcc gtgctgccgt tcgccgtcga ggagttcctg cgcttctacg
     1201 cccccgtcac catggcccgg atcgccgcca gcgacaccga gatcggcggc tgccccgtga
     1261 gcgagcggga ctgggtcctg ctccccttcc cggccgccaa ccgcgacccc gaggcgttcg
     1321 agcgggccga cgagttcatc atcgaccggc agcgcaaccg ccacgtcgcc ttcggcctcg
     1381 gcatccaccg ctgcgccggc tccaacctgg cccgcatgga gctcaccgtc gccatcgacg
     1441 agtggatgaa gcggatcccc gacttcgagc tggccgacga cgaccccgat gccgtccgct
     1501 ggtccaccgg ccaggtccgc ggcccccgcg agctgccggt ccggatcctg cgcaccaacg
     1561 ctcccgagac ggcggaggcg gcaggctgat gcgcatcgcc ttcgaccgcg agaagtgcca
     1621 gggccacggc cgctgctacg cgctggcccc cgatctgttc gacgccgacg acgagggcta
     1681 tgccgtcgtc ttggaggcag gcgacctcac cgaggcgcag ctggccgatg cccagctcgc
     1741 cgccgacaac tgccccgagt acgccatctc gatcgacgtc tgatcgtccg ccggcaccgc
     1801 gtcagcgaga ggccaggtcg gcgatggtca cggcgctgaa gccggagcgg accgcccgtt
     1861 cggcgcgcac ccagacgtcg agggtgccgt cgaagccagg tgcctcggtg ggcgtccgca
     1921 cgtcgaccgc gtcgaagacg tcggccacgg tgatctcgtg ggcggggcgc gccagccagt
     1981 agccgccgtt cgagccgcgg cggctgccca cgacctcggc gagccggagg tcgttcaagg
     2041 cggcggcgac gaacttccgc gagagcccct ggggcgcgca gagctcgcgg atcttcaggc
     2101 tggtgccctg gggtgcggcg gccaggacca cgagggcgtg gagcgcgtgg tcgaccaacg
     2161 acggggcgcg cacgaaccgc agcgtcggcc gctcgggcgc gcagcccgcg gcatcgtcgc
     2221 cccgtcctcc ccccacgtca gcccccgagc gctcccgcca gcgccagcgc ctcggccgcg
     2281 gcggcagcag ccgaagcgtg gatcccagcg gaggcctggc cgtcgtcgcc gaagtccgcc
     2341 cgggtggcgt agatgccggt cggcgtcgtc caacccccga gcgaagcgat caggggacgc
     2401 aggccgtggt cgatcgccag cgcgtgctcg gcggcgtcgc cggtggcgac ggggatcacc
     2461 accgtgccgg ccagggcggc tcggggctgc aggtcgaaga cggtcttggt gagggcggtg
     2521 taggtcgccc ggtacaccgg ggtcgcgacc acgaggatcg aagcggcggc gacccgcctc
     2581 accgccgcat ccacgtccgg ggacgagcgc agcgccaaga ggccagccgg atccaggtcg
     2641 accaggtcga gcacgtcgcc gccgccgagc agctcgagga cgtggaccgc gagggtccgg
     2701 ctgcgcgacg ggtgcgacgg gctcccgttg acgacgaggc ggcccgccat cagcgggccg
     2761 cggccagcgc cggctcggcg gcggccgggt cggcggtggg tacggggatg ccgggctcgg
     2821 cggcgatgcc cgccgccttc agcttgggga tgacgccctc accgaaccag taggcctcct
     2881 cgaggtgcgg ctgtcccgag aggatgacgt ggtcgaagcc gatctcgtgg tactcggcca
     2941 ggcgctcggc cacctcgtcg tggctgccga ccagcg
//
LOCUS       DATMFS010000602         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_66674, whole genome shotgun sequence.
ACCESSION   DATMFS010000602 DATMFS010000000
VERSION     DATMFS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66674"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1126)
                     /gene="tgt"
                     /locus_tag="VNQ33_11550"
     CDS             complement(5..1126)
                     /gene="tgt"
                     /locus_tag="VNQ33_11550"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWJ98787.1"
                     /translation="MKLHVQLDATDGNARTGTITTPRGVLPTPLFMPVGTRASVKALD
                     AADLDAMGIPLILGNTYHLMLRPGSELIAALGGLHRFMDWSGHVLTDSGGFQIFSLEP
                     KVTDEGARFRSTYDGTPTFLCPEDAARIQSELGGDIQMVLDVCPPLPSTDRVVRAAVE
                     RTAAWAVRGRQAFEELRGWREDRSLDQAQFGIAQGGVDLAMRAEHAQRTVEVGFDGYA
                     IGGLSVGEPRHEMLPALAAALEHLPADQPRYLMGVGDPASIVEAVGLGVDLFDCVLPT
                     RHARHGTVLTSEGRLMIKNLAYERDEGPLDATCSCSVCARYSRAYLRHLHRLGEPTGA
                     RLLTIHNVAWLSDLVVGLRAAISEGRFEAARRAVLDVWG"
     gene            complement(1123..>2057)
                     /gene="queA"
                     /locus_tag="VNQ33_11555"
     CDS             complement(1123..>2057)
                     /gene="queA"
                     /locus_tag="VNQ33_11555"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWJ98788.1"
                     /translation="VQHRTVADLPGLLDHGDVLLVNDTRVIPARLHLRKPTGGAVEVL
                     LLERRPEGHWEALVKPSRRVADGTVLHAEAEAGAGGAVELTVEVGPVLGGDGRRQVTI
                     HGGTDDDLAVLGRVGEVPLPPYIHEPLADPERYQTVFAQHPGSVAAPTAGLHLTDAVL
                     DACRERGVAVAPIELTVGLGTFRPITTDRVEDHPMHAERYRIPVATQEVLTGRQGRVI
                     AVGTTVVRAVESWAVTGEAEAATSLFIRGDYPFAVIDRLLTNFHVPRSSLLALVQSFT
                     GPRWKDLYRTALDEGYRFLSFGDAMLLDRPQDPR"
BASE COUNT          286 a          693 c          813 g          265 t
ORIGIN      
        1 ggcttcatcc ccatacgtcg aggacggcac gacgggcggc ttcgaagcgg ccctcgctga
       61 tggcggcgcg caggccgacg accaggtcgg acagccaggc cacgttgtgg atcgtcaaca
      121 ggcgcgcacc ggtgggctcg cccaggcggt ggaggtggcg gaggtacgca cgggagtagc
      181 gggcgcacac cgaacaggag caggtcgcgt cgagcggccc ctcgtcgcgc tcgtaggcca
      241 ggttcttgat catgagccgg ccctcggagg tcaagacggt cccgtggcgg gcgtgccggg
      301 tgggcaagac gcagtcgaac aagtcgacgc ccagcccgac cgcctccacg atgctggccg
      361 ggtcgcccac acccatgagg tagcgaggct ggtccgccgg gaggtgctcg agcgcggcgg
      421 cgagcgcggg gagcatctcg tggcgcggct cccccaccga cagcccgccg atggcgtagc
      481 cgtcgaagcc gacctcgacg gtccgctggg cgtgctcggc gcgcatcgcc aagtcgacgc
      541 cgccctgcgc gatgccgaac tgcgcctggt cgaggctgcg gtcctcccgc cagccccgca
      601 gctcctcgaa ggcctggcgg ccgcggaccg cccacgccgc ggtgcgctcg accgcggccc
      661 ggaccacccg gtcagtagac ggcagcggcg ggcacacgtc gagcaccatc tggatgtcgc
      721 cgcccagctc ggactggatg cgggcggcgt cctccggaca gaggaaggtg ggcgtgccgt
      781 cgtaggtgga gcggaacctg gcgccctcgt cggtgacctt cggctcgagg gagaagatct
      841 ggaagccgcc ggaatcggtg agcacgtggc cggaccagtc catgaaccgg tggaggccgc
      901 cgagcgccgc gatcagctcc gagccgggcc ggagcatcag gtggtacgtg ttcccgagga
      961 tgagcgggat gcccatggcg tcgaggtcgg cggcgtcgag cgccttcacc gaggcccggg
     1021 tgcccaccgg catgaacaac ggcgtcggca gcacgccccg cggcgtggtg atggtgccgg
     1081 tgcgggcgtt gccgtcggtg gcgtcgagct ggacgtggag cttcatcggg gatcctgcgg
     1141 gcggtcgagg agcatggcgt cgccgaacga gaggaaccgg tagccctcgt cgagggccgt
     1201 ccggtagagg tccttccagc gggggccggt gaaggactgg accagggcga gcagcgacga
     1261 gcggggcacg tggaagttcg tgagcagccg gtcgatcacg gcgaaggggt agtcgccacg
     1321 gatgaacaag ctggtggccg cttcggcctc gccggtgacc gcccaggact ccacggcgcg
     1381 gaccacggtg gtgcccacgg cgatgacccg gccctgccgg ccggtcagga cctcctgcgt
     1441 ggccaccggg atccggtagc gctcggcgtg catcgggtgg tcctcgacgc ggtcggtggt
     1501 gatgggccgg aacgtgccga gcccgacggt cagctcgatc ggggcgacgg ccaccccgcg
     1561 ctcgcggcag gcgtcgagca cggcgtcggt gaggtggagg cctgcggtcg gggcggccac
     1621 cgagccgggg tgctgggcga agacggtctg gtaccgctcg ggatcggcca gcggctcgtg
     1681 gatgtacggc gggaggggca cctcgccgac ccggccgagc acggccaggt cgtcgtcggt
     1741 gccgccgtgg atcgtgacct gccgccggcc gtcaccgccc aggaccggtc cgacctcgac
     1801 cgtgagctcg actgctcccc cggctccggc ctcggcctcg gcgtgcagga cggtgccgtc
     1861 ggcgacccgg cggctgggct tcaccagcgc ctcccagtgg ccctcgggcc ggcgttcgag
     1921 gagcagcacc tcgacggcgc cgcccgtggg cttgcggagg tgcaggcgcg ccgggatgac
     1981 ccgggtgtcg ttcaccagga ggacgtcgcc gtggtcgagg agcccgggca ggtcggcgac
     2041 cgtgcggtgc tggaccg
//
LOCUS       DATMFS010000603         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_300443, whole genome shotgun sequence.
ACCESSION   DATMFS010000603 DATMFS010000000
VERSION     DATMFS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300443"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNQ33_11560"
     CDS             <1..392
                     /locus_tag="VNQ33_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ98789.1"
                     /translation="GADDYLAKPFELAELLARLRALQRRTVRAASPVLRFGSLELDPA
                     QVRVTRAGRPVVLTPREFAVLAVLMRADGAVVSAEQLFEQAWDERTDPFTASVRVIVS
                     RLRSKLGEPALVETVVTKGYRLVEASP"
     gene            389..1675
                     /locus_tag="VNQ33_11565"
     CDS             389..1675
                     /locus_tag="VNQ33_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ98790.1"
                     /translation="MRLGLRQRVALWCAALVVVSGVSVVVLVVVLSGRFLAQENRPSK
                     LGLRPEQIGAPPGSAAAPSGPVAGGVDPNAARGGPTAAQGAQRAAADRTFERVRNLGL
                     ATVAGLMLASLLVAWAVAGRIVRPIKQVTETARSIGRDRKLDRRIAYDGPQDEIHDLA
                     DEFDTMLGRLEAVFDAQREFVANASHELRTPLAVIRTEVDVALDDPDASDTDLRGALH
                     AVGDVVERTSGLVTAMLALSRAEVISEPRTVDLADVARAATYAERVRNGNRSFSSDLE
                     PAPVAGDPVLLGQLVENLVRNAVTYNRDGGSVAVRIRSTGGTVQLVVENDGPPVDPRL
                     IPSLFERFVRRTPVGDGHGLGLAICATIARTHGGTIRAAPGERGGLAVTVELPAVEGT
                     TRAEHALVGAAAPDGTSSSGAAPDHGAGSTVSARRR"
     gene            complement(1656..2249)
                     /locus_tag="VNQ33_11570"
     CDS             complement(1656..2249)
                     /locus_tag="VNQ33_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWJ98791.1"
                     /translation="MSRELRRVAVFCGSNHGNDPAFTEAAVALGTLLAERGIGLVYGG
                     GDVGLMGEVADAVLAGGGEVVGVLPGLLWDKEVGHQGLTELLIVDTMHERKMAMADRA
                     DAFIALPGGVGTFEELFEAITWTQLGIHDKPVGLLDVAGFYRPLLTFLDDTVASGFLK
                     PGHRSMIVDATDPAEILAALAAWEPVTTSKWLTADER"
     gene            complement(2246..3352)
                     /locus_tag="VNQ33_11575"
     CDS             complement(2246..3352)
                     /locus_tag="VNQ33_11575"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWJ98792.1"
                     /translation="MADGISLDLIRQAPKVLLHDHLDGGLRPTTVIDIAEEVGHELPT
                     DDPAELQAWFTEGASRRNLDLYLQTFEHTVACMQTREHIERVAAECIEDLAADGVVYA
                     EVRMAPELCIEGGLTPDEAVEAMLAGFEKASAGRPIEVGLLVTAMRHAARSTEIAEVA
                     LRHRDQGVVGFDIAGSESGNPPSRHLDAFRIVAQANSHITIHAGEAFGLPSIWEALQI
                     CGAERLGHGVRIIDDIEVEPDGQVSLGRLASFVRDRRVPLEMCPTSNVHTGVCDTVAE
                     HPIGLLKQLRFRVTVNTDNRLMSDISLSKEFANLHEAFGWDWDDFEWLTINAMKSSFW
                     PFDKRLRIINEQIKPGYATLKAAGLGTSSFRVTP"
BASE COUNT          469 a         1245 c         1289 g          526 t
ORIGIN      
        1 tcggcgccga cgactacctc gccaagccgt tcgagctggc cgagctgctc gcccgcctgc
       61 gtgccctcca gcgacgcacg gtccgagccg cgagcccggt gctccgcttc gggtcgctgg
      121 aactggatcc cgcccaggtg cgggtcaccc gagcgggccg gccggtcgtg ctcacccctc
      181 gcgagttcgc cgtgctcgcc gtcttgatgc gagccgacgg agcggtcgtg agcgccgagc
      241 agctgttcga gcaggcgtgg gacgagcgca ccgatccgtt caccgcgtcg gtgcgcgtca
      301 tcgtctcccg gttgcgttcc aagctcggcg agccggcgct cgtcgagacc gtcgtgacca
      361 aggggtaccg cttggtggag gcgtcgccgt gaggctcggg ctgcgccagc gcgtggcgct
      421 gtggtgcgcg gcgctggtgg tggtctccgg cgtcagcgtc gtcgtgctcg tcgtcgtgct
      481 ctccggtcgc ttcctggccc aggagaacag gcccagcaag ctcgggctca ggcccgagca
      541 gatcggcgcc ccgcccgggt cggcggcggc tccgagcggt ccggtggccg gcggggtcga
      601 tccgaacgcg gcgcgaggcg ggccgaccgc agcgcaaggc gcccagcgcg ctgccgccga
      661 ccgcaccttc gagcgcgtgc gcaacctcgg cctcgccacg gtcgccggct tgatgctggc
      721 gagcctgctg gtggcgtggg ccgtggccgg gcggatcgtg cggccgatca agcaggtcac
      781 cgagacggcg cgctcgatcg gccgtgaccg gaagctcgac cgccgcatcg cctacgacgg
      841 gccccaggac gagatccacg acctggccga cgagttcgac accatgctcg gccggctgga
      901 ggcggtgttc gacgcgcagc gggagttcgt ggccaacgca tcccacgagc tgcggacgcc
      961 gctggctgtg atccgcaccg aggtcgacgt cgccctcgac gatccggacg catccgacac
     1021 cgacctgcgc ggtgccctgc acgcggtggg tgatgtggtc gagcgcacga gcgggctggt
     1081 cacggcgatg ctggcgctgt ctcgcgccga ggtcatctcc gagcctcgga ccgtcgacct
     1141 ggccgacgtc gcgcgcgccg ccacctacgc cgagcgcgtg cgcaacggga accgctcgtt
     1201 ctcgtcggac ctcgagcccg ccccggtggc cggagacccc gtcctcctcg gccagctggt
     1261 ggagaacctg gtccgcaacg ccgtcaccta caaccgagac ggcggatccg tggccgtgcg
     1321 catccgctcg accgggggca cggtccagct cgtggtggag aacgacggtc ctccggtcga
     1381 tccccggctc atcccatcgc tgttcgaacg gttcgtgcgc cggacgccgg tgggcgatgg
     1441 ccacgggctc ggcctggcga tctgcgccac gatcgcccgc acgcacggcg gcacgatccg
     1501 agcggcgccg ggcgagcgtg gcggcctggc cgtgaccgtc gagctccctg ccgtcgaggg
     1561 caccactcgc gccgagcacg ccctcgtcgg agccgctgcg cccgatggca cgtcgtcatc
     1621 gggcgcagca cccgatcacg gcgccgggtc gacggtcagc gctcgtcggc ggtgagccac
     1681 ttcgacgtcg tcaccggctc ccacgcggcc agcgcggcga ggatctcggc ggggtcggtg
     1741 gcgtcgacga tcatcgaccg gtggccgggc ttgaggaagc ccgacgcgac cgtgtcgtcg
     1801 aggaacgtga gcagcgggcg gtagaagccg gcgacgtcga gcaggccgac gggcttgtcg
     1861 tggatgccga gctgggtcca ggtgatggcc tcgaacagct cctcgaacgt gccgacgccg
     1921 ccgggcaggg cgatgaacgc atcggcccgg tcggccatcg ccatcttgcg ctcgtgcatg
     1981 gtgtcgacga tcagcagctc ggtgagcccc tggtggccga cctccttgtc ccacagcagc
     2041 ccggggagca cgccgacgac ctcgccgcct cctgccagga ccgcgtcggc gacctcgccc
     2101 atcaagccga cgtcgccgcc gccgtagacg aggccgatgc cgcgctcggc gaggagggtc
     2161 cccagcgcga cggccgcctc ggtgaacgcg gggtcgttcc cgtggttgga gccgcagaag
     2221 accgcgaccc gcctcagctc gcggctcacg gcgtgacgcg gaacgacgag gtcccgaggc
     2281 cggccgcctt gagcgtggcg tagcccggct tgatctgctc gttgatgatc cgcagccgct
     2341 tgtcgaacgg ccagaagctg ctcttcatgg cgttgatcgt cagccactcg aagtcgtccc
     2401 agtcccaccc gaacgcctcg tgcaggttgg cgaactcctt cgacagcgag atgtcgctca
     2461 tgagccggtt gtcggtgttg acggtgaccc ggaaccgcaa ctgcttgagc aggccgatgg
     2521 ggtgctcggc cacggtgtcg cacacaccgg tgtggacgtt cgacgtgggg cacatctcca
     2581 gcgggacccg gcggtcgcgg acgaacgagg ccagccggcc gagcgacacc tggccgtcgg
     2641 gctccacctc gatgtcgtcg atgatccgga ccccgtggcc gagccgttcg gcaccgcaga
     2701 tctgcagggc ctcccagatg gacgggaggc cgaacgcctc tccggcgtgg atcgtgatgt
     2761 gcgagttggc ttgggcgacg atgcggaacg cgtcgaggtg gcggctgggc gggttgccgg
     2821 actcgctgcc ggcgatgtcg aagcccacca cgccctggtc gcggtggcgc agggccacct
     2881 cggcgatctc ggtggagcgg gcggcgtggc gcatggcggt gacgagcagg ccgacctcga
     2941 tgggccgacc ggcgctggcc ttctcgaacc cggcgagcat ggcctcgacc gcctcgtcgg
     3001 gtgtcaggcc gccctcgatg cacagctccg gggccatgcg cacctcggcg tagacgacgc
     3061 cgtcggcagc caggtcctcg atgcactcgg ccgccacccg ctcgatgtgc tcgcgggtct
     3121 gcatgcaggc gacggtgtgc tcgaacgtct ggaggtacag gtccaggttg cggcggctgg
     3181 cgccctcggt gaaccaggcc tggagctccg cggggtcgtc ggtcggcagc tcgtgaccga
     3241 cttcctcggc gatgtcgatc acggtggtgg gccgcaaccc gccgtcgagg tggtcgtgca
     3301 ggagcacctt cggggcctgt cggatcagat cgagggagat gccgtcagcc atggacggag
     3361 ggtaccggtg ccctccgtcg tccgcctgcg gattcccccg cccgccaccg tccgttcgcc
     3421 gagacgccgc ctggcagtgt caggaggtca ggcagaacgg gtgtccggcc ggatccagga
     3481 ggatccgcca acgtccatcg ccgggctgat cgtccggttt ggtcgcgcc
//
LOCUS       DATMFS010000604         5025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_274328, whole genome shotgun sequence.
ACCESSION   DATMFS010000604 DATMFS010000000
VERSION     DATMFS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274328"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..723)
                     /gene="rdgB"
                     /locus_tag="VNQ33_11580"
     CDS             complement(130..723)
                     /gene="rdgB"
                     /locus_tag="VNQ33_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWJ98793.1"
                     /translation="MGERTPIVLASANPKKAAEIAEILADHLELVPRPAEVPDVIEDA
                     STFEGNARLKAVALCEATGLAALADDSGLEVDALGGAPGVWSARYAGEDATDEQNVDE
                     LLAALADRPDPADRTARFRAVVVLRYSDGRERVAAGHVEGRIADAPRGPGGFGYDPVF
                     VPAEGDGRTFGEMTQDEKHAISHRGRALRDLLAQLDA"
     gene            complement(733..1464)
                     /gene="rph"
                     /locus_tag="VNQ33_11585"
     CDS             complement(733..1464)
                     /gene="rph"
                     /locus_tag="VNQ33_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWJ98794.1"
                     /translation="MRADGRQNDELRPIRFERDFTVQAAGSVLVSFGGTRVLCTASVD
                     ENVPRWMKGKGKGWVTAEYSMLPGASAERINREAAKGKQSGRTQEIQRLIGRSLRSVC
                     DMKALGERQVIVDCDVLQADGGTRTASICGGYVALHDALTRTLQAGGLATHPLTTECA
                     AISVGVIGGVPMLDLPYVEDATAETDMNVVMTGTGGFVEVQGTAEGAPYSRTELDALL
                     DLAEGGIAQIVELQRAVLATPPAPR"
     gene            complement(1525..2370)
                     /gene="murI"
                     /locus_tag="VNQ33_11590"
     CDS             complement(1525..2370)
                     /gene="murI"
                     /locus_tag="VNQ33_11590"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWJ98795.1"
                     /translation="MTADRTDDRPIGMFDSGFGGLTVARAFLDLLPDEDLVYVGDTGR
                     YPYGPRSLDEVAGYAHEIARFLVDEHDVKLLVVACNTAAAAALGSLRDQLDVPVMGVI
                     DPGVRSLVRASETGRVGVIGTVGTVGSGAYQRAVAASGAPVELTVAACPGFVEFVEGG
                     ETDSDQVHVLAERLLAPVVEAKVDALLLGCTHYPYLARTITDVVGRDVVLVSSADETA
                     FEALALFHQPSPGPGEPALEPATQGRVGAHRFVSSGDVDRFRDLGARLLGPELAAAER
                     APWSP"
     gene            complement(2410..3846)
                     /locus_tag="VNQ33_11595"
     CDS             complement(2410..3846)
                     /locus_tag="VNQ33_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ98796.1"
                     /translation="MLKRLVAPAAALVGALLAVRRGHEAIAFAILVGAFFLAQLAWVA
                     PAVMDRIDAGLRRGVQLLLHGLVGLLGSLLLLPLWALFKVVRVDPIRPASRVPGRWRR
                     IEGPATIDTYSSMPAPSGSERWRVRLGGAAVIAAVLGLVYVLPIPIPGISSSSSDDAV
                     PSGLTRPVVEDDGGIPYRAFAFADEPWGKALMDERAKAPLVADPETIWQSGDFAGTYH
                     NVKDRVRRSWTPDDPQLTVWFLGGSAAWGVGQRDGRTIPSMLARDAAADGIRLKVVNM
                     AVPAFTSWQETATLRRELARQDPPDLVIVYHGSNDIGTLDARASEGITPLDRPGNLMN
                     AYAYGFAPPTTGVPVADVDAIARGGLRILKEETRAMTDAAEAAGVPIDFAWQPSLWSG
                     GPNRFDKDAIAANGLYPESLARRRAMEDQIVAGLPPGLIDLRTSLDDAEKPTFFDIVH
                     TNEYGAALIADALWKQIAPQVRMLASAS"
     gene            4003..4689
                     /locus_tag="VNQ33_11600"
     CDS             4003..4689
                     /locus_tag="VNQ33_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ98797.1"
                     /translation="MTGERLLLVDDEDNLRSMLEAALRHSGFEVHPAANGRDALDAVP
                     EVKPDLIVLDVMLPDLDGFDVCQRLRSAGDRTPVLFLTARDATEDKVRGLTMGGDDYL
                     VKPFSLDELVARIHAVLRRTGNAKEGAVLACADLELDDEAHLVTRAGVEASLSPTEYN
                     LLRYLLLNQGRVLSKAQILDHVWQYDFGGDGGVVETYIGYLRKKVDSVEPRLIQTVRG
                     VGYTIREPRT"
     gene            4694..>5025
                     /locus_tag="VNQ33_11605"
     CDS             4694..>5025
                     /locus_tag="VNQ33_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98798.1"
                     /translation="MSLRARLLLGMAVVAVVLVVASLSITRATEHNLIRQVDQQLARS
                     GPQARQGNYGSGGQDGGPPSALYIARFNRRTGELNTLYLPSGTDSASPPDLTFGQATE
                     HDGRPAFTV"
BASE COUNT          723 a         1816 c         1807 g          679 t
ORIGIN      
        1 tggcccgccc acgtcgatcc caccgtctcg cagcacgagc agctgtgggt ccaccaggct
       61 ggcgaggtcg tcgactgctg gcccatcgac ctccgcggct ggtagcgccg gtccgagcga
      121 gaccccgcgt caggcgtcga gctgggcgag caggtcgcgg agggcgcggc cgcggtggct
      181 gatcgcgtgc ttctcgtcct gggtcatctc gccgaacgtg cggccgtcgc cttcggcggg
      241 gacgaacacc gggtcgtagc cgaagccgcc cgggccgcgg ggtgcgtcgg cgatgcggcc
      301 ctcgacgtgc ccggcggcca cccgctcgcg gccgtcggag taccggagca cgacgacggc
      361 acggaagcgg gccgtgcggt cggccgggtc gggacggtcg gccagggcgg cgaggagctc
      421 gtcgacgttc tgctcgtcgg tggcgtcctc cccggcgtag cgggccgacc agacgcccgg
      481 tgcgccgccg agggcgtcga cctcgagacc cgagtcgtcg gcgagcgcgg ccagacccgt
      541 ggcctcgcac agcgcgacgg ccttgagccg agcgttgccc tcgaaggtgc tcgcatcttc
      601 gatcacgtcg ggcacctcgg cgggccgggg caccagctcc aggtgatcgg cgaggatctc
      661 ggcgatctcg gcggccttct tcgggttggc cgacgccaag acgatgggcg tgcgctcccc
      721 caccgggccg cctcagcggg gcgccggcgg cgtggccagc acggctcgct ggagctcgac
      781 gatctgggcg atgccgccct cggccaggtc gagcagcgcg tcgagctcgg tccgggagta
      841 cggcgcaccc tcggcggtgc cctgcacctc gacgaagccg cccgtccccg tcatcaccac
      901 gttcatgtcg gtctcggccg tggcgtcttc gacgtacggc aggtcgagca tcggcacgcc
      961 gccgatgaca ccgaccgaga tggccgcgca ctcggtggtc agcgggtggg tggcgagacc
     1021 accggcctgc agggtccggg tgagggcgtc gtgcagggcc acgtagccgc cgcagatcga
     1081 cgcggtgcgg gtgccgccgt cggcctggag gacgtcgcag tcgacgatga cctggcgctc
     1141 gcccagggcc ttcatgtcgc agaccgagcg cagcgagcgg ccgatgagcc gctggatctc
     1201 ctgggtgcgg ccggactgct tgcccttggc ggcctcgcgg ttgatgcgct cggcggaggc
     1261 gcccgggagc atcgagtact cggcggtgac ccagcccttg cccttgccct tcatccaccg
     1321 cggcacgttc tcgtcgaccg acgccgtgca cagcacccgg gtgcccccga acgacaccag
     1381 caccgatccg gcggcctgca ccgtgaagtc gcgctcgaag cggatgggtc ggagttcgtc
     1441 gttctggcgg ccgtcggcgc gcatggtggc tcctgcagaa ggtggggaag gcgggccgcc
     1501 catcgtcgca gacccgcccc agcttcaggg ggaccagggg gcgcgctcgg cggcggcgag
     1561 ctcggggccg aggaggcggg caccgaggtc gcggaagcgg tcgacgtcgc ccgacgacac
     1621 gaaccggtgg gcaccgactc ggccctgggt cgccggttcg agcgccggct cgccgggtcc
     1681 aggcgacggc tggtggaaca gggcgagcgc ctcgaacgcg gtctcgtcgg ccgaggacac
     1741 gagcaccacg tcgcggccga cgacgtcggt gatggtgcga gccaggtacg ggtagtgcgt
     1801 gcacccgagc agcagggcgt cgaccttggc ctccacgacc ggggcgagga ggcgctcggc
     1861 gaggacgtgg acctggtcgc tgtcggtctc gccgccctcg acgaactcaa cgaagcccgg
     1921 gcaggcggcg acggtgagct cgactggtgc cccgctcgcg gccaccgccc gctggtaggc
     1981 gccggagccg accgtcccga ccgtgccgat gaccccgacc cgcccggtct cggacgcgcg
     2041 cacgagggag cggaccccgg ggtcgatcac gcccatgacc ggcacgtcga gctggtcgcg
     2101 taggctgccc agcgctgcgg cggccgcggt gttgcaggcg accacgagga gcttcacatc
     2161 gtgctcgtcc accaggaacc gggcgatctc gtgggcgtac cccgcgacct cgtcgagcga
     2221 gcgcggtccg tacgggtagc ggccggtgtc gccgacgtac acgaggtcct cgtcgggcag
     2281 caggtccagg aacgcccgag ccacggtgag gccgccgaac ccgctgtcga acatcccgat
     2341 gggacggtcg tcggtgcgat cagcggtcac cgccccaggc tacggccgcg gcccttcccg
     2401 cccccagctt caggacgcgc tggcgagcat ccggacctgg ggggcgatct gcttccagag
     2461 ggcgtctgcg atcagcgccg ccccgtactc gttggtgtgg acgatgtcga agaacgtcgg
     2521 cttctcggcg tcatcgagcg aggtccgcag gtcgatgagc cccgggggca gaccggcgac
     2581 gatctggtcc tccatggcgc gccgacgagc gagggattcg gggtagagcc cgttggcggc
     2641 gatcgcgtcc ttgtcgaacc ggttcggtcc gcccgaccac agcgacggct gccaggcgaa
     2701 gtcgatcggc accccagccg cctccgccgc atccgtcatc gcccgcgtct cctccttcag
     2761 gatgcgcagc ccgccccggg cgatggcatc cacgtcagcg accggcacgc ccgtggtcgg
     2821 cggggcgaag ccgtaggcgt aggcgttcat caggttgccg ggccggtcga gcggcgtgat
     2881 cccctcggac gcgcgggcgt cgagggtacc gatgtcgttc gagccgtggt acacgatgac
     2941 gagatccggt ggatcctggc gggccagctc ccgccgcaag gtcgccgtct cctgccagct
     3001 cgtgaacgcc ggcacggcca tgttgaccac cttcaagcgg atgccgtcgg ccgcggcgtc
     3061 ccgggccagc atcgacggga tggtgcgtcc gtcccgctgg ccgacgcccc aggccgccga
     3121 accgccgagg aaccagaccg tcagctgcgg gtcgtccggg gtccatgatc gccggacccg
     3181 gtccttgacg ttgtggtacg tcccggcgaa gtccccgctc tgccagatcg tctccggatc
     3241 ggcgacgagc ggcgccttgg cccgctcgtc catcagggcc ttgccccacg gctcgtcggc
     3301 gaaggcgaac gcccggtagg ggataccgcc gtcgtcctcg acgaccggac gggtgaggcc
     3361 gctgggcacc gcatcgtcgc tcgacgacga gctgatgccg gggataggga tggggagcac
     3421 gtagaccaga ccgaggacgg ccgcgatcac cgcagcaccg ccgaggcgga cgcgccagcg
     3481 ctccgagccg ctcggtgccg gcatcgagga gtaggtgtcg atcgtcgcgg gcccctcgat
     3541 ccgacgccac cgaccgggca cccggctggc ggggcggatg gggtccaccc gcaccacctt
     3601 gaacagtgcc cacagcggca gcaggagcag gctgccgagc aggccgacga gaccgtggag
     3661 caggagctgc accccacggc gaagcccggc gtcgatgcgg tccatcaccg ccggtgccac
     3721 ccacgccagc tgtgccagga agaaggcgcc cacgaggatg gcgaaggcga tggcctcgtg
     3781 cccgcgccga acggccagca gggcgccgac gagcgccgcg gcgggggcca ccagcctctt
     3841 cagcacgtcc tccgtccccc tgtgatcgcc acggcgtggt gcctcctgaa ccggttggcg
     3901 aaccctaact cggggtggcc gcaccacctc cgcgaccggg actgacgagg agtccagagg
     3961 attctcagga acttcccagg aacaggtgcg aggctggtgg tcgtgaccgg tgaacgactc
     4021 ctgctcgtcg acgacgagga caacctgcga tcgatgctcg aggcggccct gcgccacagc
     4081 ggcttcgagg tccaccctgc cgccaacggc cgcgacgccc tcgacgccgt gcccgaggtg
     4141 aagcccgacc tgatcgtgct cgacgtgatg ctgccggacc tcgacgggtt cgacgtgtgc
     4201 cagcggctcc gcagcgccgg cgaccggacg cccgtcttgt tcctcacggc acgtgacgcc
     4261 accgaggaca aggtccgcgg cctcacgatg ggcggcgacg actacctggt gaagccgttc
     4321 agcctggacg agctcgtcgc ccgcatccac gccgtgctgc gacgcaccgg caacgccaag
     4381 gagggggcgg tgctggcctg cgctgacctg gagctcgacg acgaagctca cctcgtgacc
     4441 cgtgccggcg tcgaggcctc gctctcgccc accgagtaca acctcttgcg ctacctcctg
     4501 ctcaaccagg gccgggtgct gtcgaaggcg cagatcctcg atcacgtgtg gcagtacgac
     4561 ttcggcggcg acggcggcgt ggtcgagacc tacatcgggt acctccgcaa gaaggtcgac
     4621 tccgtcgagc cccgcctcat ccaaacggtc cgaggggtgg gctacacgat ccgcgaaccg
     4681 cggacctgac gcgatgtcgc tgcgcgcccg cctgctgctc ggcatggccg tcgtggccgt
     4741 ggtcctcgtc gtcgcctcac tctcgatcac gcgcgccacc gagcacaacc tcatccgcca
     4801 ggtcgaccag caactggccc gctccggtcc ccaggcccgc cagggcaact acggcagcgg
     4861 gggccaagac ggaggaccgc cgagcgcgct ctacatcgcc cggttcaacc ggcggaccgg
     4921 cgagctgaac accctgtacc tgccgtcggg caccgactcc gccagtccgc ccgacctcac
     4981 cttcggccag gccaccgagc acgacggccg gccggcgttc accgt
//
LOCUS       DATMFS010000605         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_347083, whole genome shotgun sequence.
ACCESSION   DATMFS010000605 DATMFS010000000
VERSION     DATMFS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347083"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VNQ33_11610"
     CDS             <1..844
                     /locus_tag="VNQ33_11610"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HWJ98799.1"
                     /translation="AARHEQRRSMGIAKRLPGAAASLAGAAVTDPVGSAKRLGETASS
                     VAKLAAPAVDPLSPVMTHRSLSVHFDTVSLSLSEAKAAAKAAGGRLNDAFLAATAAGL
                     RHYHQELGHPVDAVRISMPINVRDADDPEATGNHFAPARFPLPLTIEDPKEAMTTIRE
                     IVQAERAEPALALVDPLATILNRLPTSMTTTLFGAALRGVDVVASNVPGAPVELFTAG
                     ARINSMFALGPMAGAAVNLTLLSYLDQVHIGINVDPAAVTEPDRFLAAYRAGWDEVLA
                     AAGA"
     gene            888..2102
                     /locus_tag="VNQ33_11615"
     CDS             888..2102
                     /locus_tag="VNQ33_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWJ98800.1"
                     /translation="MTEPTRADAAIVGAGPAGAAAAITLARAGRSVVLIDKAVFPRDK
                     ICGDGITTGALRILEELGVRPGTIPSWTEVADIHVVSPSKHLATFPLPRGRGQFAAVM
                     RRTDLDDAIVDQAREAGVRMIEGVEVTAAADGADEVALDLSDGSTVVAAHVIAADGMW
                     SPMRKFLGTAVPGYLGEWHAFRQYFTATGPKARDLWVWFEPDLLPGYAWSFPLPDGGA
                     NVGFGIQRGAKIATKEMKALWPELLARPHIAEVLGPGAVPEDRHQAWPIPARTGDLAL
                     SAGRVLWVGDAAAVTDPMTGEGIGQALLTGQLAAQAIVDGGADPAAIRTAYEHAVRSH
                     LLADHKMSLLLVRALKHRKGARAAIRVAGATPWTRRNFARWLFEDEPRAIAVTPRRWH
                     RRILRRDGAYVG"
     gene            2107..2367
                     /locus_tag="VNQ33_11620"
     CDS             2107..2367
                     /locus_tag="VNQ33_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HWJ98801.1"
                     /translation="MSQLETTVDRSVEQTTDEQTDDGDHEKFAHVVVPASAVTEAYIT
                     GTPVTALCGKTWVPTRDPNRYPVCPTCKEVLELARNLRDQHG"
BASE COUNT          330 a          983 c          795 g          308 t
ORIGIN      
        1 tgccgcccgg cacgagcagc gccgcagcat gggcatcgcc aagcgcctgc ccggcgcagc
       61 ggcgtcgctc gccggcgcgg cggtcaccga tccggtcggg tcggccaagc gcctgggcga
      121 gaccgcgtcg tcggtcgcca agctggcggc gcccgccgtc gatcccctct cccccgtcat
      181 gacccaccgc tccctgtcgg tgcacttcga caccgtctcg ctctcgctct ccgaggcgaa
      241 ggcggccgcc aaggcggccg gcgggcggct caacgacgcc ttcctcgccg ccaccgcggc
      301 cgggctgcgg cactaccacc aggagctggg ccatcccgtc gacgccgtgc gcatctcaat
      361 gccgatcaac gtgcgcgacg ccgacgaccc cgaggccacg ggcaaccact tcgcccccgc
      421 ccgcttcccg ctgccgctca ccatcgagga cccgaaggag gccatgacca cgatccggga
      481 gatcgtgcag gccgagcggg ccgagccggc actggccctg gtggacccgc tggcgaccat
      541 cctcaaccgc ctgcccacgt cgatgaccac gacgctgttc ggcgccgccc tccgcggcgt
      601 cgacgtggtg gccagcaacg tgcccggcgc ccccgtcgag ctgttcaccg ccggggctcg
      661 gatcaactcg atgttcgccc tcgggccgat ggccggcgcc gccgtgaacc tcacgctgct
      721 ctcgtacctg gaccaggtcc acatcgggat caacgtcgac ccggcggcgg tcaccgagcc
      781 cgaccggttc ctcgcggcgt accgcgccgg gtgggacgag gtcctggccg cggcaggtgc
      841 ctgatcgagt tggagccgtc ccggcccggc gccgtaccct ccgaacgatg accgagccga
      901 cccgcgccga cgcggccatc gtgggcgccg gacccgccgg tgccgccgcc gcgatcaccc
      961 tcgcccgagc cggccgcagc gtggtcctca tcgacaaggc cgtcttcccc cgcgacaaga
     1021 tctgcggcga cggcatcacc acgggggcgc tgcgcatcct cgaggagctc ggcgtacgtc
     1081 ccggcaccat cccgtcgtgg accgaggtgg cggacatcca cgtcgtgtcg ccgtcgaagc
     1141 acctggcgac cttcccgctc ccgcggggcc gcggccagtt cgccgccgtc atgcgccgca
     1201 ccgacctcga cgacgccatc gtcgaccagg cacgcgaagc cggggtgcgg atgatcgagg
     1261 gcgtggaggt gaccgccgcg gccgacggag ccgatgaggt cgccctcgat ctgtcggacg
     1321 gctcgaccgt cgtcgccgcg cacgtgatcg ccgccgacgg catgtggtcg ccgatgcgga
     1381 agttcctcgg caccgcggtg cccggctacc tgggtgagtg gcacgcgttc cgccagtact
     1441 tcaccgccac cggccccaag gcccgtgacc tgtgggtgtg gttcgagccc gacctgctgc
     1501 ccggctacgc ctggtcgttc ccgctgcccg acggcggggc caacgtcggc ttcggcatcc
     1561 agcgcggcgc caagatcgcc accaaggaga tgaaggccct gtggcccgag ctcctggccc
     1621 ggccccacat cgccgaggtg ctcggccccg gcgccgtccc cgaggaccgc caccaggcct
     1681 ggcccatccc cgcccgcacc ggcgacctgg ccctctcggc cgggcgcgtg ctctgggtcg
     1741 gcgacgccgc ggcggtcacc gacccgatga ccggcgaggg catcggccag gccctgctca
     1801 ccggccagct cgccgcccag gccatcgtcg acggtggcgc cgaccccgcg gcgatccgga
     1861 ccgcgtacga gcacgccgtg cggtcgcacc tgctcgccga tcacaagatg tcgctgctgc
     1921 tggtccgggc actgaagcac cgcaagggcg cccgggccgc catccgcgtg gccggcgcca
     1981 ccccctggac ccgccgcaac ttcgcccgtt ggctgttcga agacgagccc cgggccatcg
     2041 ccgtcacgcc ccggcgctgg caccgtcgca tcctccggcg cgacggcgcc tacgtaggct
     2101 gatgccatga gccagctcga gaccaccgtc gaccgctcgg tcgagcagac caccgacgag
     2161 cagaccgacg acggcgacca cgagaagttc gcccacgtgg tggtcccggc atccgcggtc
     2221 accgaggcct acatcaccgg cacccccgtg accgctctct gcggcaagac ctgggtcccc
     2281 acccgagacc ccaaccgcta cccggtgtgc cccacctgca aagaggtcct cgagctggcc
     2341 cggaacctcc gcgaccagca cggctagctg cgctcggtca cgacggcgag gcccctcgcc
     2401 ctccctgtga tgccgg
//
LOCUS       DATMFS010000606         3663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21101, whole genome shotgun sequence.
ACCESSION   DATMFS010000606 DATMFS010000000
VERSION     DATMFS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21101"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VNQ33_11625"
     CDS             complement(<1..285)
                     /locus_tag="VNQ33_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98802.1"
                     /translation="MGDDHPGEPAWKRDFPYESAGEDDVTRREFARYLVAGAGVMAAG
                     NLGLAAWTQLRTINTGEPRELIPLDDVAIGGTHLFRYPADADPAILLRLAD"
     gene            complement(285..908)
                     /locus_tag="VNQ33_11630"
     CDS             complement(285..908)
                     /locus_tag="VNQ33_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ98803.1"
                     /translation="MKATRYGIDDRPVFAIDPSRCIGCEACVQACAECGTHRGQSLIH
                     LERIDRTASTQTAPMVCMHCEDPTCAQVCPADAIKQNEEGIVQSALKPRCIGCSNCVI
                     ACPFGVPKYVADFDQMMKCDMCTDRTSEGYAPMCASVCPSEALWYGTPEEFHATRHGE
                     LLDDWLFGRQAVHTKVFAVVDDAAGGPLDVLGATTGTWLDDPFGLEG"
     gene            complement(905..3094)
                     /locus_tag="VNQ33_11635"
     CDS             complement(905..3094)
                     /locus_tag="VNQ33_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HWJ98804.1"
                     /translation="MARLPITEAGLIEAYGPHLNEAPPGGWDAGLEVDEVVETHCCFC
                     GQQCGIKLKVHDNAVVGFEPWYDFPFNEGKLCPKGVKRYLQGSHPDRLLHPMVRDESA
                     PGGFRDVSWDEALSRVASEIQRIQATYGDDAFAMLSGVSLTNEKSYLVGKFARLALHT
                     ANLDYNGRYCMVSAGVGNKKALGIDRAPNPWSDIPLADVVWVAGSNVAETFPITTSYI
                     WRARDRGAKLIVQDPRVVPLARTADLFLPVRPGSDSALFGAVLHELIRNDWLDHEFIA
                     AHTVDFDQAAAAAADMTPAWAADITGVPAARIEEAAELWGTAATGMMLHARGIEQQTK
                     GVDNVLAAINLGLATGKFGKPGCGVSTITGQGNGQGGREHGHKCDQLPGNRDISNPEH
                     REAVAKVWEVDESEIPGKGIPAQEIIEAIHDGTIKGLLSICFNPAVSSPDSNFTKEAL
                     DKLEFYSVIDFFLSETAQHADVVLAGSLHEEDEGTSTSGEGRIIKINAAVTPPGEARL
                     DWEILLEIAERLGKGDHFRYGSTHEIFEELCRASSGGTADYTGATWERVEAEHGLFWP
                     IPEVGHPGTPRLHEGGTFSHPDGKARFHGVPYRPPAEVVDDEYPIWLTTGRVVSQYLS
                     GAQTRSIASLVAQYPEPLCEIHPKLAERLGIAHGDQVTVRSRRGEITLPAAVVTTIRP
                     DTVFIPYHWPGRKAANQLTIRAVDPLSKMPEFKVAAVHVARAAGRPA"
     gene            complement(3094..>3663)
                     /locus_tag="VNQ33_11640"
     CDS             complement(3094..>3663)
                     /locus_tag="VNQ33_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98805.1"
                     /translation="GTAEFDAGTVVGWVIFHGLDIAAVLVLAGVFIFLRRRLHDPGAL
                     AVERGGDFLVLAGLFAVSVTGLFLTVSSVWLDGQFYSALNTMHALTVILGLMYLPFGK
                     LFHIFQRPGNLGVAYYRTANEEGPAAVCRRCGGEFASARQVADLQEVLPQVGFDYGIE
                     GGGSYQDTCPRCRRASVTLMQSARVGGFG"
BASE COUNT          513 a         1178 c         1387 g          585 t
ORIGIN      
        1 gtcggcgagg cgcaggagga tggccgggtc ggcgtcggcg gggtagcgga agaggtgcgt
       61 gccgccgatc gcgacgtcgt cgagggggat cagctctctg ggttcgccgg tgttgatggt
      121 gcgcagctgg gtccaggcgg cgaggccgag gttgccggcg gccatcacac cggcgccggc
      181 gacgaggtac cgggcgaact cgcgccgggt cacgtcgtcc tcgccggcgg actcgtaggg
      241 gaagtcgcgc ttccaggcgg gttcgcccgg gtggtcgtcg cccatcagcc ctccagtccg
      301 aacgggtcgt cgagccacgt gccggtcgtg gcgccgagta catcgagggg acctccggca
      361 gcgtcgtcga ccacggcgaa gaccttggtg tgcacggcct ggcggccgaa cagccaatcg
      421 tcgaggagct caccgtgccg ggtggcgtgg aactcctcgg gcgtcccgta ccagagggcc
      481 tcgctggggc agaccgacgc gcacatgggg gcgtagccct cggaggtgcg gtcggtgcac
      541 atgtcgcact tcatcatctg gtcgaagtcg gcgacgtact tggggacacc gaacggacag
      601 gcgatcacgc agttggagca gccgatgcag cgaggcttga gcgcggattg cacgatgccc
      661 tcctcgttct gcttgatggc atcggcgggg cacacctggg cgcaggtcgg atcctcgcag
      721 tgcatgcaga ccatgggggc ggtctgggtg ctggcggtgc ggtcgatgcg ctcgaggtgg
      781 atgagcgact ggccgcggtg ggtgccgcac tcggcgcagg cttggacgca tgcctcgcag
      841 ccgatgcacc ggctgggatc gatggcgaag acgggccggt cgtcgatgcc gtagcgggtg
      901 gccttcatgc cggccgtccc gcggctcggg cgacgtgcac ggcggcgacc ttgaactcgg
      961 gcatcttcga gagcgggtcc acggcccgga tcgtgagctg gttggccgcc ttgcgcccgg
     1021 gccagtggta cgggatgaac accgtgtcgg gccggatcgt ggtgacgacg gcggcgggca
     1081 gggtgatctc gccgcgccgc gaccgcacgg tgacctggtc gccgtgcgcg atgccgaggc
     1141 gctcggccag cttcgggtgg atctcgcaca gcggctcggg gtactgcgcg acgaggctgg
     1201 cgatgctgcg ggtctgggcg ccggacaggt actggctgac gacccggccg gtggtgagcc
     1261 agatcgggta ctcgtcgtcg acgacctcgg ccgggggccg gtaggggacc ccgtggaagc
     1321 gcgccttgcc gtcggggtgg gagaacgtcc cgccctcgtg gaggcgcggg gtgcccgggt
     1381 ggcccacctc ggggatgggc cagaacaggc cgtgctcggc ctcgacgcgc tcccaggtgg
     1441 cgccggtgta gtccgcggtg ccgcctgacg acgcccggca gagctcctcg aagatctcgt
     1501 gggtgctccc gtaccggaag tggtcgccct tgccgagccg ctcggcgatc tcgagcagga
     1561 tctcccagtc gagccgggct tctcccggtg gggtgaccgc ggcgttgatc ttgatgatgc
     1621 ggccctcgcc gctggtggag gtgccctcgt cctcctcgtg cagggagccg gcgaggacga
     1681 cgtcggcgtg ctgggcggtc tcggagagga agaagtcgat gaccgagtag aactcgagct
     1741 tgtcgagcgc ctccttcgtg aagttgctgt cgggcgatga caccgccggg ttgaagcaga
     1801 tcgacagcag tcccttgatg gtgccgtcgt ggatggcctc gatgatctcc tgggcgggga
     1861 tgcccttgcc ggggatctcg ctctcgtcga cctcccacac cttggcgacc gcttcgcggt
     1921 gctcggggtt ggagatgtcg cggttgccgg ggagctggtc gcacttgtgc ccgtgctccc
     1981 gccctccttg gccgttgccc tggccggtga tggtcgacac gccgcagccg ggcttgccga
     2041 acttgccggt ggccaagccc aggttgatgg ccgccagcac gttgtcgacg cccttggtct
     2101 gctgttcgat gccccgggcg tgcagcatca tcccggtggc ggccgtgccc cacagctcgg
     2161 ccgcctcctc gatgcgcgcc gcgggcacgc cggtgatgtc ggcggcccag gctggggtca
     2221 tgtcggcggc ggccgccgca gcctggtcga agtcgacggt gtgggcggcg atgaactcgt
     2281 ggtcgagcca gtcgttgcgg atcagctcgt ggagcaccgc gccgaacagc gccgagtcgc
     2341 tcccgggccg gaccgggagg aacaggtcgg cggtgcgggc gagcggcacg acccgggggt
     2401 cttggacgat gagcttcgcc ccgcggtcgc gggcgcgcca gatgtagctc gtcgtgatcg
     2461 ggaacgtctc ggccacgttg gagcccgcca cccagacgac gtcggcgagg gggatgtcgc
     2521 tccacgggtt cggcgcccgg tcgatcccga gcgccttctt gttgccgacg ccggccgaca
     2581 ccatgcagta gcggccgttg tagtcgaggt tggcggtgtg gagcgccagg cgggcgaact
     2641 tgccgaccag gtagctcttc tcgttggtga gcgagacgcc cgacaacatg gcgaacgcgt
     2701 cgtcgccgta ggtggcctgg atgcgctgga tctcgctggc cacccgggac agcgcctcgt
     2761 cccagctcac gtcgcggaac ccgcctggag ccgactcgtc tcgcaccatg gggtgcagca
     2821 ggcgatcggg gtggctgccc tgcaggtagc gcttgacgcc cttggggcag agcttgccct
     2881 cgttgaacgg gaagtcgtac cagggctcga acccgacgac cgcgttgtcg tggaccttca
     2941 gcttgatgcc gcactgctgc ccgcagaagc agcagtgggt ctcgacgacc tcgtcgacct
     3001 ccaggccggc gtcccacccg ccggggggtg cctcgttcag gtgcggaccg taggcctcga
     3061 tgagacctgc ttcggtgatc gggagacggg ccatcagccg aaacctccga ctcgtgcgga
     3121 ctgcatcaag gtgacgctcg cccgtcggca ccgcgggcag gtgtcctggt agctgccgcc
     3181 gccttcgatg ccgtagtcga agccgacctg gggcaggacc tcttggaggt cggcgacctg
     3241 tcgggcgctg gcgaactccc cgccacagcg ccggcacacg gccgccggcc cctcctcgtt
     3301 ggcggtccgg tagtaggcga cacccaggtt cccaggacgc tggaagatgt ggaacagctt
     3361 cccgaacggg aggtacatca gcccgaggat cacggtgagg gcgtgcatcg tgttgagcgc
     3421 tgagtagaac tgcccgtcca gccacacgct ggacacggtg aggaacaggc cggtgaccga
     3481 tacggcgaag aggccggcga gcaccaggaa gtcgccgccg cgctccacgg cgagcgcccc
     3541 ggggtcgtgg aggcggcggc ggaggaagat gaacacgccg gcgagcacca agaccgcggc
     3601 gatgtcgagg ccgtggaaga tcacccagcc caccaccgtg cccgcatcga actcagcggt
     3661 ccc
//
LOCUS       DATMFS010000607         5530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_90984, whole genome shotgun sequence.
ACCESSION   DATMFS010000607 DATMFS010000000
VERSION     DATMFS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90984"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="map"
                     /locus_tag="VNQ33_11645"
     CDS             <1..570
                     /gene="map"
                     /locus_tag="VNQ33_11645"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWJ98806.1"
                     /translation="PPFPAVICASPNDMVVHGVPSDEVVLADGDIVSIDCGAVVDGWH
                     GDAAFTAAVGEVPAEVQHLIEVTEASLAAGIAAMVEGNRIGDIGAAIEQVALKAGLGV
                     VEGYTGHAIGQAMHEAPNIPNQGTPGTGARLRAGNVLAIEPMLVLGTPDTAILDDDWS
                     VVTADGRWAAHAEHTIAITSEGPQILTLL"
     gene            841..1065
                     /gene="infA"
                     /locus_tag="VNQ33_11650"
     CDS             841..1065
                     /gene="infA"
                     /locus_tag="VNQ33_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWJ98807.1"
                     /translation="MPKPKEDAIVLEGTVLEAQKNAMFRVELQNGHEVLAHISGKMRM
                     HYIRILPGDKVQVELTPYDLARGRITYRYK"
     gene            1111..1227
                     /gene="rpmJ"
                     /locus_tag="VNQ33_11655"
     CDS             1111..1227
                     /gene="rpmJ"
                     /locus_tag="VNQ33_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWJ98808.1"
                     /translation="MKVQPSVKPICEHCKVIRRHGRVQVICSSNPRHKQRQG"
     gene            1231..1608
                     /gene="rpsM"
                     /locus_tag="VNQ33_11660"
     CDS             1231..1608
                     /gene="rpsM"
                     /locus_tag="VNQ33_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWJ98809.1"
                     /translation="MARIAGVDIPREKRLVISLTYVFGIGRTTAEKICEATGIDESTR
                     TRDLTDEEINKIRAYIDQNLKVEGDLKREVQQDIKRKMEIGSYQGLRHRKGLPVRGQR
                     THTNARTRKGPKKTVAGKKKVRK"
     gene            1608..2006
                     /gene="rpsK"
                     /locus_tag="VNQ33_11665"
     CDS             1608..2006
                     /gene="rpsK"
                     /locus_tag="VNQ33_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWJ98810.1"
                     /translation="MAKPKPGGRRPRKRERKNVTYGVAHIKSSFNNTIVSITDQEGNV
                     ISWASAGNVGFKGSRKSTPFAAQMAAEQAARRAMEHGVRKVDVMVKGPGSGRETAIRS
                     IQNTGIEVTGIKDVTPVPHNGCRPPKRRRV"
     gene            2018..2632
                     /gene="rpsD"
                     /locus_tag="VNQ33_11670"
     CDS             2018..2632
                     /gene="rpsD"
                     /locus_tag="VNQ33_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWJ98811.1"
                     /translation="MARYTGPKVKISRRLGVNIFENDKGSKALNKRPYPPGEHGRSRR
                     RQPSEYLLQLQEKQKAKYIYGVLERQFSNLYKEASRQKGVTGENLLRMLEQRLDNMVF
                     RAGWASTRPQARQFVSHGMVNVNGKRVDIPSYRVRKGDVISLRDKSRQMIVIRHNMDT
                     LDRLKPNWFEMSGDGFELTVNELPVREQIDVPVREQLIVELYSK"
     gene            2665..3603
                     /locus_tag="VNQ33_11675"
     CDS             2665..3603
                     /locus_tag="VNQ33_11675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWJ98812.1"
                     /translation="MLVIQRPTVEAVGEEENNRQRFSISPLEPGFGHTLGNSLRRTLL
                     SSIPGAAITAVRFDDALHEFDTITGITEDVTDIILNLKDLVLTSVSDEPVTLRIDVRG
                     AAEVTGADIQTTADVEVLNAQQHIATLNDKARLALDLTIERGRGYLSADRDKGNNTIG
                     VIPVDAIFSPVRRVTFLVEPTRVEMDTNYDRLVLDVETDGSIAPRDAVASAGDTLAKL
                     VQLVAEVSDEPPTLELGETSATMASSPELEMPIEDLDLSERPRNCLKRAQVNTVGELL
                     RKSEDDLLAITNFGQKSLDEVKEKLNERGLALRGME"
     gene            3606..3959
                     /gene="rplQ"
                     /locus_tag="VNQ33_11680"
     CDS             3606..3959
                     /gene="rplQ"
                     /locus_tag="VNQ33_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWJ98813.1"
                     /translation="MPATPRRGRRFGGSSQHQKLMMANLVSSLIAAEGITTTEAKAKA
                     MRPVAEKMITKAKKGGLHNHRQVVAYLNDKEIAAKLFDDVGPRYSDRNGGYTRILKLG
                     PRQGDNAPMARIELV"
     gene            complement(4185..5069)
                     /gene="sigJ"
                     /locus_tag="VNQ33_11685"
     CDS             complement(4185..5069)
                     /gene="sigJ"
                     /locus_tag="VNQ33_11685"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HWJ98814.1"
                     /translation="MITDRAVSGDAEFEQERPRLEGLAYRITGSRATAEDVVQDAWLR
                     WERSDREAVERPAAWLTTVTSRLALDHLKSAQRTRETYVGPWLPEVAVADPGPAERAE
                     LAESVTIGFLTVLERLGPTERVVFLLADVFATPFEEIAAIVGKSPEACRQIASRARTR
                     VRDGRPRFEPTGEDAWQVALAFLTAAQVGDLDALVSLLADDALAISDGGADHHAARRP
                     VIAERIPRFIVNLTARLPEDAELVAMSINGEPGIVTYVDGEPTVAVAVSIADGQVERV
                     WSILNPEKLKHLTSTPIV"
     gene            complement(5066..>5530)
                     /locus_tag="VNQ33_11690"
     CDS             complement(5066..>5530)
                     /locus_tag="VNQ33_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ98815.1"
                     /translation="TAACLELGIRGLPTHRFALGIDEPLYGSTHCPPADLAPEGHAVV
                     HLMRYQPVGDHVPATEQRARLEQLARQMGIAPDAVVEQRFLAKMVVAGTLPHPATGGL
                     AGRPPVDVAEHPGVFLAGDWVGPTGLLLDAVASSAVEAGRRAASRSATMARA"
BASE COUNT          990 a         1953 c         1830 g          757 t
ORIGIN      
        1 ccgccgttcc cggcggtgat ctgcgcgtcg cccaacgaca tggtcgtcca cggcgtcccg
       61 tccgacgagg tggtcctcgc cgacggcgac atcgtgtcga tcgactgcgg agccgtcgtc
      121 gacggatggc acggcgatgc ggcgttcacc gcggcggtgg gcgaggtccc tgccgaggtg
      181 cagcacctga tcgaggtcac cgaggcctcg ctcgccgccg gcatcgccgc catggtcgag
      241 ggcaaccgga tcggcgacat cggtgcggcc atcgagcagg tcgccctgaa ggccgggctc
      301 ggggtcgtcg agggctacac cggccacgcc atcggccagg cgatgcacga agcgcccaac
      361 atcccgaacc agggcacgcc gggtacgggt gcccgcctgc gcgcgggcaa cgtgctggcc
      421 atcgagccga tgctcgtgct gggcaccccc gacacggcga tcctcgatga cgactggagc
      481 gtggtgaccg ccgacggtcg ctgggcggcc cacgccgagc acaccatcgc catcacgtcc
      541 gagggtcccc agatcctcac cctgctttga gctcggcccg cacggcatcc acccgtccgc
      601 ccgcaggcgc cggcgccttc tggcatgatg tcgcgcacgc agcggggccg accgggcccc
      661 gaaaccggcc cgagcgggcc gcaacgggat tgccacaccc ctgccacacg gttgtggtag
      721 tgcgccaact tggatgtcgt gggccttcgc gttacgatcg tccttcggcc cgcggggcct
      781 gcgcgctttc gagcgtccag gcacgggctg gttcacgcag gacctaccag gagatttccg
      841 ctgcccaagc ccaaggaaga tgcaatcgtc ctcgaaggga cggtgctcga agcgcagaag
      901 aacgcgatgt tccgcgtgga gctgcagaac gggcacgagg tgctcgcgca catctccggg
      961 aagatgcgca tgcactacat ccggattctc cctggtgaca aggtccaggt cgaactcacc
     1021 ccctatgacc tggcccgagg ccggatcacc taccgctaca agtagccggc tcggcggacc
     1081 cgcccgccga ccatcgatca cgaggcaccg atgaaggtcc aacccagcgt caaacccatc
     1141 tgcgagcact gcaaggtcat ccgtcgtcac ggacgagtcc aggtcatctg ctcctccaac
     1201 ccccgccaca agcagaggca aggctgaacc atggcacgta tcgccggcgt cgacatcccc
     1261 cgcgagaagc gtctcgtcat ctccctcacc tacgtgttcg gcatcggccg gaccaccgca
     1321 gagaagatct gcgaggcgac ggggatcgac gagagcacga ggacccgcga cctcaccgat
     1381 gaagagatca acaagatccg tgcgtacatc gaccagaacc tgaaggtcga aggcgatctc
     1441 aagcgcgagg tccagcagga catcaagcgc aagatggaga tcggctcgta ccagggtctg
     1501 cgccaccgca agggcctccc ggtccgcggc cagcgcaccc acaccaacgc tcggacccgc
     1561 aagggcccga agaagaccgt tgccggcaag aagaaggttc ggaagtaatg gcgaagccaa
     1621 agccgggcgg tcgccgcccc cgcaagcgcg agcggaagaa cgtcacctac ggcgtggcgc
     1681 acatcaagag ctcgttcaac aacacgatcg tgtccatcac cgatcaagag ggcaacgtga
     1741 tctcgtgggc ctcggcgggc aacgtcgggt tcaagggctc ccgcaagtcc accccgttcg
     1801 ccgcccagat ggccgccgag caggcggcgc gccgggccat ggagcacggc gtccgcaagg
     1861 tcgacgtgat ggtcaagggc ccgggctccg gtcgggagac cgccatccgg tccatccaga
     1921 acaccggcat cgaggtcacc ggcatcaaag acgtgacccc cgtgccccac aacggctgcc
     1981 gaccgcccaa gcggcgccgg gtctgaggta gaggaacatg gctcgttaca ccggtcccaa
     2041 ggtcaagatc tcgcgccgtc tcggcgtgaa catcttcgag aacgacaagg gctccaaggc
     2101 cctcaacaag cgcccgtacc cgccgggtga gcacggccgc agccgtcgcc gccagccgtc
     2161 cgagtacctg ctccagctcc aggagaagca gaaggcgaag tacatctacg gggtcctgga
     2221 gcgccagttc tccaacctgt acaaggaagc cagccgccaa aagggcgtca ccggcgagaa
     2281 cctgctccgc atgctcgagc agcgcctcga caacatggtc ttccgcgccg gctgggcgtc
     2341 cacccgcccg caagctcgcc agttcgtgag ccacgggatg gtcaacgtca acggcaagcg
     2401 ggtcgacatc cccagctacc gcgtccgcaa gggcgacgtc atctcgctgc gcgacaagtc
     2461 gcgccagatg atcgtgatcc gccacaacat ggacaccctc gaccggctca agccgaactg
     2521 gttcgagatg agcggcgatg gcttcgagct gaccgtgaac gaactgcccg tccgcgagca
     2581 gatcgacgtg cccgtccgtg agcagctcat cgtcgagctg tactccaagt aatccgcacc
     2641 gcagaacccc gaggaaggca atacatgctg gtcattcagc gccccacggt cgaggcagtc
     2701 ggcgaggagg agaacaaccg ccaacggttc tccatctcgc cgctcgagcc cggcttcggc
     2761 cacacgctcg gcaactcgct gcgccggacc ctgctctcgt cgatccccgg cgccgccatc
     2821 acggcggtcc gcttcgacga tgcgctccac gagttcgaca cgatcacggg catcaccgag
     2881 gacgtcaccg acatcatcct caacctcaag gacctggtcc tcacgtcggt gtccgacgag
     2941 ccggtcaccc tgcgcatcga cgtgcgcggt gcggccgagg tcaccggtgc cgacatccag
     3001 accacggcgg acgtcgaggt gctcaacgcc cagcagcaca tcgccacgct caacgacaag
     3061 gcccgcctcg cgctggacct caccatcgag cgggggcgcg gctacctgtc cgccgaccgc
     3121 gacaagggca acaacaccat cggtgtgatc cccgtcgacg ccatcttctc gccggtgcgc
     3181 cgcgtgacct tcctggtcga gcccacccgc gtcgagatgg acaccaacta cgaccgcctc
     3241 gtcctcgacg tcgagaccga cggctccatc gctcctcgtg atgccgtcgc ctccgccggc
     3301 gacaccctgg ccaagctcgt gcagctcgtg gccgaggtga gcgacgagcc gccgaccctc
     3361 gagctcggcg agaccagcgc caccatggcg tcctcgccgg agctggagat gcccatcgaa
     3421 gacctcgatc tctccgagcg tccccgcaac tgcctcaagc gcgcccaggt caacaccgtc
     3481 ggcgagctcc tgcgcaagag cgaggacgac ctcctggcca tcaccaactt cggccagaag
     3541 tccctcgacg aggtcaagga gaagctgaac gagcggggcc tggcgctccg cgggatggaa
     3601 tagcaatgcc tgcaacccca cgccggggac gccgcttcgg cggctcctcg cagcaccaga
     3661 agctgatgat ggccaacctg gtctcgtcgc tcatcgccgc cgagggcatc accaccaccg
     3721 aggccaaggc caaggccatg cgcccggtgg cggagaagat gatcaccaag gcgaagaagg
     3781 gcggcctcca caaccaccgc caggtggtgg cctacctgaa cgacaaggag atcgcagcca
     3841 agctgttcga cgacgtcggc ccgcgctact ccgatcgcaa cggcggctac acccgcatcc
     3901 tcaagctcgg cccccgccag ggcgacaacg cccccatggc gaggatcgag ctcgtctgag
     3961 ttgctcgttt gaaagaatcc gccgcccctc cggggcggcg gatttctttc aagccgaacg
     4021 tgaggacggg ctgaagcccg acgattccgc agctcggcac gacttcgtcg cacctcgctg
     4081 accgaagggg ctccgcaagc tccgccccct cggactcccc cgggctcgct tcgctcggga
     4141 accacaaccc cacctcgtgt ggggttgttg catttttgcc ggggttagac gatgggggtg
     4201 ctggtgaggt gcttcagctt ttcggggttg aggatgctcc agacccgctc gacctggccg
     4261 tcggcgatgc tcacggcgac ggccacggtc ggttcgccgt cgacgtaggt gacgatgccg
     4321 ggttcgccgt tgatggacat ggcgacgagc tcggcgtctt ccgggaggcg ggcggtgagg
     4381 ttgacgatga agcgcgggat ccgctcggcg atgaccgggc ggcgggccgc atggtggtcg
     4441 gcgccgccgt cggagatggc gagggcatcg tcggccagga gcgagacgag ggcgtcgagg
     4501 tcaccgacct gagcagcggt gaggaacgcc agcgccacct gccaggcgtc ctcgccggtc
     4561 ggttcgaacc gcggccggcc gtctcggacg cgggtacggg cgcgcgaggc gatctggcgg
     4621 cacgcctccg gcgacttgcc gacgatggcg gcgatctcct cgaacggcgt ggcgaacacg
     4681 tcggcgagga ggaacacgac ccgctcggtc gggcccagcc gctcgagcac ggtcaagaag
     4741 ccgatcgtca ccgattcggc cagttcggcg cgctcggccg gcccggggtc ggccaccgcc
     4801 acctccggca gccaggggcc gacgtaggtc tcccgggtgc gctgtgcgga cttcaggtgg
     4861 tcgagggcca accggctcgt gaccgtggtc agccaggccg cgggacgctc gacggcctcg
     4921 cgatcggacc gctcccagcg cagccaggca tcctgcacga cgtcttcggc ggtggcccga
     4981 gacccggtga tccggtacgc gaggccctcg agccgggggc gctcctgctc gaactcggcg
     5041 tcgccggaga cggcgcggtc ggtgatcatg cgcgcgccat ggtggccgaa cgagaagcgg
     5101 cgcgacgacc ggcctcgacc gcgctcgagg cgacggcatc gagcaggagg ccggtcggac
     5161 ccacccagtc gccggcgagg aacacgccgg ggtgctcggc gacgtcgacc ggtggccggc
     5221 cggcgaggcc gccggtggcc ggatggggga gcgtgccggc gaccaccatc ttggcgagga
     5281 agcgctgctc gaccacggca tcgggcgcga tgcccatctg gcgggcgagc tgctcgagcc
     5341 gggctcgctg ctcggtggcg gggacgtggt cgccgacggg ctggtaccgc atcaggtgga
     5401 cgacggcgtg gccctcggga gccagatcgg ccggtgggca gtgggtcgat ccgtagagcg
     5461 gctcgtcgat gcccagggcg aaccggtggg tcggcagccc ccggatcccc agctccaggc
     5521 aggcggcggt
//
LOCUS       DATMFS010000608         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_251619, whole genome shotgun sequence.
ACCESSION   DATMFS010000608 DATMFS010000000
VERSION     DATMFS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251619"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VNQ33_11695"
     CDS             complement(<1..725)
                     /locus_tag="VNQ33_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ98816.1"
                     /translation="MTDTPWLGDACSLVEAFRAKERSPLEELDATLAAIEASDLNAFV
                     HVDEQRARAAAAGADTSLPFGGVPLGIKSLDSVEGWPSTEESLVFADRVAGHTSTHMA
                     RILGAGGMVPVGQTLASEFGGLNVSTNRLHGTCHNPWQHGRTAGGSSGGSSAAVAGGL
                     VTIASGGDGGGSIRIPAAFNGLVGMKGTAGRIPRGPRTQVNPMTVVVGCQARSVRDVA
                     RWYDVATGYDSRDPYSLPRIDGW"
     gene            complement(766..1062)
                     /locus_tag="VNQ33_11700"
     CDS             complement(766..1062)
                     /locus_tag="VNQ33_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HWJ98817.1"
                     /translation="MTMRHVVCFRFQPDTDPGQVDALAAGLRELPAIIPELAAYQVGP
                     DLGIGEGTWDFAVVADFASTDDFAVYRDHPEHQARIQALVLPITAERVAVQYAT"
     gene            complement(1111..1869)
                     /locus_tag="VNQ33_11705"
     CDS             complement(1111..1869)
                     /locus_tag="VNQ33_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98818.1"
                     /translation="MDLGISGKRAVVAASSAGLGLASARALAAEGVQVAICGRSRDRV
                     DAALETLPAVPTGDHAGLVADVGSADGATSFVERAVAALGGIDVLVPNAGGPPPGNFA
                     STPLDAYLPALQLNLLSTVAMCQAAVPALVDQGWGRIIAITSHSVRQPVADLILSNTT
                     RAGATGFLKTLATEIARHGVTVNSVQPGVHATDRMRQLHAGAPGAAASGVPTGVVGRP
                     EDFGAVVAFLCSDHARFITGTAVPVDGGAFQSLQ"
     gene            2017..>2212
                     /locus_tag="VNQ33_11710"
     CDS             2017..>2212
                     /locus_tag="VNQ33_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWJ98819.1"
                     /translation="MDAREFLGLQPTHNPHRWHFAVTPGLSTGGGFLFGGCGLGAAIA
                     ALEGTTDRPLVWATAQYLNYA"
BASE COUNT          284 a          775 c          867 g          286 t
ORIGIN      
        1 tcccagccgt cgatgcgggg gagcgagtac gggtcgcggc tgtcgtagcc ggtggcgacg
       61 tcgtaccagc gggccacatc acgcaccgag cgggcctggc acccgacgac cacggtcatc
      121 gggttgacct gggtcctcgg gccgcggggg atgcggccgg cggtgccctt catgccgacc
      181 agcccgttga aggcggcggg gatgcggatg gatccgccgc cgtcgccgcc gctggcgatc
      241 gtgacgagcc cgccggccac cgcggcggag gaaccgcccg agctgccgcc cgccgtgcgc
      301 ccgtgctgcc acgggttgtg gcacgtgccg tggaggcggt tggtggacac gttgaggccg
      361 ccgaactccg aggcgagcgt ctggcccacc gggaccatcc cgccggcgcc gaggatccgc
      421 gccatgtggg tgctcgtgtg gccagcgacc cggtcggcga agaccaggga ctcctcggtg
      481 gagggccagc cctccaccga gtcgagcgac ttgatcccga gcgggacgcc gccgaacggg
      541 agcgaggtgt cggcgccggc ggctgccgct cgggcccgtt gctcgtcgac gtgcacgaag
      601 gcgttgaggt cgctggcctc gatggcggcg agggtcgcgt cgagctcctc gagcggcgag
      661 cgctccttgg cccggaaggc ctccacgagc gaacaggcgt ctccgagcca gggggtatcg
      721 gtcatggcgc ggaccctatg cggccgtgcg gccggccgcg ggtgctcagg tggcgtactg
      781 gacggcgacg cgctccgcgg tgatcggaag gaccagggcc tggatgcggg cctggtgctc
      841 cgggtggtcc cggtagacgg cgaagtcgtc ggtgctggcg aagtcggcca cgaccgcgaa
      901 gtcccacgtg ccctcgccga tgccgaggtc ggggcccacc tggtaggcgg cgagctcggg
      961 gatgatcgcc ggcagctcgc ggagcccggc ggccagggca tcgacttggc ccgggtcggt
     1021 gtcgggctgg aaccggaagc agaccacgtg gcgcatcgtc atggcgccga ccctaccgat
     1081 cggatgggcg gccggcgccg gaccgagccg ctactggagg gactggaacg cgccgccgtc
     1141 gacggggacc gcggtgccgg tgatgaagcg ggcgtggtcc gagcagagga aggcgacgac
     1201 agcgccgaag tcctccggcc ggcccaccac cccggtcggc acgccgctcg ccgcggcgcc
     1261 cggggcaccc gcgtggagct ggcgcatgcg gtcggtggcg tggacgcccg gctgcacgct
     1321 gttgaccgtg acaccgtggc gggcgatctc ggtggcgagc gtcttgagga accccgtggc
     1381 cccggcgcgg gtcgtgttcg acaggatcag gtcggcgacc ggttggcgca cgctgtggga
     1441 cgtgatggcg atgatccggc cccagccctg gtcgaccagc gcaggcaccg cggcctggca
     1501 catggcgacg gtggagagca ggttgagctg gagcgcgggc aggtacgcgt cgagcggggt
     1561 ggaggcgaag ttccccggtg gcggaccgcc cgcgttgggc acgaggacgt cgatgccgcc
     1621 gagcgcggcg acggctcgct cgacgaagct cgtggcgccg tcggccgagc ccacgtcggc
     1681 caccaggccc gcgtggtcgc cggtcggcac cgccggcaag gtctcgaggg cggcgtcgac
     1741 ccgatcccgg ctccgcccgc agatcgcgac ctgcacgcct tcggcggcga gcgcccgtgc
     1801 cgacgccaac cccaacccgg ccgacgacgc cgccaccacc gcccgcttcc cgctgatccc
     1861 gaggtccatg ccgccgaccc taccgacggg cggtggcggt tctcggcgtc gaacgacggc
     1921 cgtgaccgcc ctgctgcgag cagccgtccg gcgacgagca cggggccggt ggtcgtcggg
     1981 gccggtgccg gggcggctgg gttaggttcg ctggccgtgg atgcacgtga gttcctgggc
     2041 ctccaaccca cccacaaccc gcaccgctgg cacttcgccg tgacccccgg tctgtccacc
     2101 ggcggcgggt tcctcttcgg cgggtgcggg ttgggcgccg ccatcgccgc gctcgagggg
     2161 accacggacc gtcccctcgt gtgggcgacg gcgcagtacc tgaactacgc ca
//
LOCUS       DATMFS010000609         4204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_324082, whole genome shotgun sequence.
ACCESSION   DATMFS010000609 DATMFS010000000
VERSION     DATMFS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324082"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VNQ33_11715"
     CDS             <1..1078
                     /locus_tag="VNQ33_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWJ98820.1"
                     /translation="DSGRGSSPFMLDPQGRKVGATYLRNNFNKRSVGIDLKAEPGRDL
                     FLDLVPHFDVVAENFKAGTLERLGLGYDVIAERHPGVIHVSLSGFGSDSPYKDRAAYA
                     SIVEAMSGIYAYKTPDGPPTTIPVGALGDISSALFATIGILVALRHRERTGEGQHVDV
                     AMLDAMVAMTDLVMNLWSLGARQDPEKPLGLICEGFRCSDGYVVMQVVREHQFTKLAD
                     LVGRPEWHDDPRLADRYGWEPNLDEVIRPAVEAWSSQRTRAEAAAELAAAGIAAGPSQ
                     TAPEVIADPHVALHDMVVEMPRTDGVDEPVLVPGNPVKLSKVAEGPETRVPWLGEHTD
                     EVLAAELGLDASRLDELRAAGVIG"
     gene            1090..1911
                     /locus_tag="VNQ33_11720"
     CDS             1090..1911
                     /locus_tag="VNQ33_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWJ98821.1"
                     /translation="MDDQGDDWGDTLAGFDIGDLSPNQRQTLSTDLADAGILHAFVGP
                     KLQGPAAESELIEHLVAQTRRGGRGSRRRTSPGAAPGSTARPGTTGPLPRALRLPWGA
                     VPLEVVGLPIAAPWRRFAAYLVEAIAFNIAIALVLVYSVGGAELFSAAVGLTSALVLV
                     AAFGGTLGMMVFRMRVVAVDAPDRTAPGWRVAVVRYLVAWWPQTLVMVADPFVDFEGW
                     RWVGTLMQIWLVVCFTPILLDPARRGLHDRVAGTVVIDAFRGPFSAPSASRGATR"
     gene            complement(1874..2332)
                     /locus_tag="VNQ33_11725"
     CDS             complement(1874..2332)
                     /locus_tag="VNQ33_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ98822.1"
                     /translation="MLNRRAVILLVEDSDDDADLTVRALRRNNVVNEVVRVADGVEAL
                     DYLAATGPYADRDRSDLPELILLDLKLPRLSGLDVLEELGQNGISTQVPIVVLTSSIE
                     ERDLERAYALGANSYVQKPVDFEQFTQAVSTIGMYWLLVNESPHAMPTGR"
     gene            complement(2319..3863)
                     /locus_tag="VNQ33_11730"
     CDS             complement(2319..3863)
                     /locus_tag="VNQ33_11730"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWJ98823.1"
                     /translation="MSDEPAASVHRGTRLTTRLTIILAAIVLLAAVNAALALHAASER
                     DRARDASADLRTMSTAAAVLAQSAIDQETDQRGFLITGDPTYLDQYDEAGERIDASLA
                     SLRHQSADDPAVLRSAEDAAAALAAWRDHAANVEIQAREHEGVEGAADTVSTGRGKEL
                     FDQLRSRNAALQAEIEAQLQAARDRSTAAYDRFVLLVSITLAAVVAVAVALAIALSRW
                     ITRPVERLATSVGEVQAGHLDHPVDISGPRELVEIGTAVEDMRQRLVAELDRSRAAGE
                     ELAQANAELEAFSYSVSHDLRAPLRSIDGFSQALIEDSGDQLDEVALAHFSRIRAATQ
                     RMGALIDDLLKLSRVSRVAPVLADIDMSAIAEAVTAELAEAEPDREVEVDIQPGLRAQ
                     ADASLVQVVFENLLGNAWKFTSQSPVAHIAVRGTGPSPGTTFTVSDDGAGFDQAYADK
                     LFSPFQRLHTDRQFPGTGIGLATVARIVRRHGGNISAAGVEGEGATVTFTLARDSTME
                     TTDAEP"
     gene            3978..>4204
                     /locus_tag="VNQ33_11735"
     CDS             3978..>4204
                     /locus_tag="VNQ33_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ98824.1"
                     /translation="MDPAEPVTTIRVLLVEDSLDDAALAERSLRQPGFDFEVARVESA
                     AAFRSALTEHSWDLIVCDHGLPQFGVFEAIE"
BASE COUNT          561 a         1430 c         1547 g          666 t
ORIGIN      
        1 cgattcgggt cgggggtcga gccccttcat gctcgacccg cagggccgga aggtcggcgc
       61 cacctacctt cgcaacaact tcaacaagcg cagcgtgggc atcgacctga aggcggagcc
      121 gggccgggac ctgttcctcg acctcgtgcc gcacttcgac gtggtggccg agaacttcaa
      181 ggccggcacg ctcgagcgcc tgggcctcgg ctacgacgtc atcgccgagc gccaccccgg
      241 cgtcatccac gtgtcgctgt cggggttcgg cagcgacagc ccgtacaagg accgggccgc
      301 ctacgcctcc atcgtcgagg ccatgtcggg catctacgcc tacaagacgc ccgacggccc
      361 gcccaccacc atcccggtcg gtgcgctcgg cgacatcagc agcgccctgt tcgccaccat
      421 cggcatcctc gtggccctgc gccaccggga gcgcacgggc gagggccagc acgtcgacgt
      481 ggccatgctc gacgccatgg tcgccatgac cgacctggtc atgaacctct ggtcgctcgg
      541 tgcccgccag gatccggaga agccgttggg cttgatctgc gagggcttcc gctgctccga
      601 cgggtacgtc gtgatgcagg tcgtgcgcga gcaccagttc accaagctgg ccgatctggt
      661 gggcaggccg gagtggcacg acgatccccg cttggccgac cgctacggct gggagccgaa
      721 cctcgacgag gtcatccggc cggcggtcga ggcgtggtcg tcgcagcgca cccgggccga
      781 ggcggcggct gagctggcgg cggccggcat cgccgccggg ccgagccaga cggcgcccga
      841 ggtcatcgcc gacccccacg tcgccctcca cgacatggtc gtcgagatgc cccgcaccga
      901 tggcgtcgac gagccggtcc tcgtgccggg caacccggtg aagctgtcga aggtggccga
      961 agggcccgag acccgcgtgc cgtggctggg cgagcacacc gacgaggtcc tggcggcgga
     1021 gctgggactc gacgcgagcc ggctcgacga gctgcgcgcc gccggcgtga tcggctgagc
     1081 cggaccgtcg tggacgatca gggcgacgac tggggcgaca ccctcgccgg gttcgacatc
     1141 ggcgacctgt cgccgaacca gcgccagacc ctgtccaccg atctggccga cgccggcatc
     1201 ctccatgcct tcgtgggtcc gaagctccaa gggccggcgg ctgagtcgga gctcatcgag
     1261 cacctggtcg cccagacccg cagaggcggc cgtgggagcc ggcggcggac ctccccgggg
     1321 gccgcgccgg ggtcgacggc ccgtccgggc acgaccggac cgctgcctcg ggccctgcgg
     1381 ctgccgtggg gcgccgtgcc gctcgaggtc gtcggcctcc cgatcgcggc accgtggcgg
     1441 cggttcgccg cctacctggt ggaggcgatc gccttcaaca tcgcgatcgc tctcgtgctc
     1501 gtgtactccg tgggcggcgc cgagctgttc agcgcggcgg tgggcctcac cagtgccctg
     1561 gtgctggtcg ccgccttcgg cgggaccctc gggatgatgg tgttccgcat gcgcgtggtc
     1621 gccgtcgacg ctccagaccg caccgctccc ggttggcgcg tcgcggtcgt gcgctacctc
     1681 gtcgcgtggt ggccgcagac cttggtgatg gtggcggatc cgttcgtcga cttcgagggg
     1741 tggcggtggg tcggcacgct gatgcagatc tggctcgtcg tgtgcttcac gccgatcctg
     1801 ctcgacccgg cccgccgcgg cctgcacgac cgcgtcgccg gcacggtcgt gatcgatgcc
     1861 ttccgggggc cgttcagcgc cccgtcggca tcgcgtgggg cgactcgttg acgaggagcc
     1921 agtacatgcc gatcgtcgag acggcttggg tgaactgctc gaagtccacc ggcttttgga
     1981 cgtagctgtt cgcgccgagg gcgtaggcgc gctccaggtc gcgctcttcg atggacgagg
     2041 tgagcaccac gatggggacc tgggtgctga tcccgttctg cccgagctcc tccaagacgt
     2101 cgagtccgct caggcggggg agcttgaggt cgagcaggat cagctcgggg agatcggagc
     2161 ggtcgcggtc ggcgtagggg cccgtcgccg cgaggtagtc gagggcctcc acgccgtcgg
     2221 cgacgcgcac gacctcgttg acgacgttgt tgcggcgaag cgctcggacc gtgaggtcgg
     2281 cgtcgtcatc gctgtcttcg accaggagga tcacggctct acggttcagc atcggttgtc
     2341 tccatcgtgg aatctcgagc gagcgtgaag gtcacggtgg cgccttcgcc ctccacccct
     2401 gcggcactga tgttcccgcc gtggcgccgg acgatgcgcg cgaccgtggc cagaccgatc
     2461 ccggtgccgg ggaactgccg atcggtgtgc agccgctgga acggggagaa gagcttgtcg
     2521 gcgtaggcct ggtcgaaccc ggccccgtcg tcgctgaccg tgaacgtggt ccccggcgat
     2581 ggccccgtcc ctcgtacggc gatgtgggcc acggggctct gggacgtgaa cttccacgcg
     2641 tttcccagga ggttctcgaa gacgacctgg acgagcgagg cgtcggcttg ggcacggaga
     2701 cctggttgga tgtcgacctc gacctcacgg tccggctcgg cctcggccag ctcggcggtc
     2761 accgcttcgg cgatggcgga catgtcgatg tcggcgagca cgggcgcgac gcgggagacc
     2821 cgcgacagct tgagcaggtc gtcgatcagc gcgcccatgc gctgggtcgc ggcgcggatc
     2881 cggctgaagt gggcgagggc gacctcgtcg agctggtcgc ctgagtcctc gatgagggcc
     2941 tggctgaacc cgtcgatcga gcgcaacggc gcccggaggt cgtgggagac cgagtagctg
     3001 aaggcctcca gctccgcgtt cgcctgcgcc agctcctcgc cggcagcgcg ggatcgatcg
     3061 agctccgcca ccagtcgctg tcgcatgtcc tcgaccgcgg tgccgatctc caccagctcc
     3121 cgcggcccgg agatgtcgac ggggtggtcg aggtggccgg cttggacctc gcccaccgac
     3181 gtcgccaggc gctcgacggg gcgggtgatc cagcgcgaga gggcgatcgc caacgccacg
     3241 gccaccgcga ccaccgccgc gagcgtgatg gacacgagga gcacgaaccg gtcgtacgcc
     3301 gcggtcgagc ggtcgcgagc ggcttggagc tgcgcttcga tctcggcctg cagggcggcg
     3361 ttgcggctgc ggagctggtc gaagagctcc ttgcccctgc ccgtcgagac cgtgtctgcc
     3421 gccccctcga cgccttcgtg ctcccgggct tggatctcga cgttggcggc gtggtcgcgc
     3481 cacgctgcga gggcggccgc ggcgtcttcg gcggatcgca agaccgcggg atcgtccgcc
     3541 gactggtggc gcaggcttgc gagggacgcg tcgatgcgtt cgcccgcctc gtcgtactga
     3601 tcgaggtagg tcgggtcccc ggtgatgagg aagccccgtt ggtcggtctc ctggtcgatc
     3661 gcggactgag cgagcaccgc ggccgcggtg ctcatcgtcc ggagatcggc ggatgcgtcc
     3721 cgagcccggt cgcgctcgga tgcggcgtgg agcgcgaggg ccgcgttcac ggcggcgagc
     3781 agcacgatcg cggcgaggat gatggtgagg cgcgtcgtga gacgggtccc gcgatgcacc
     3841 gaggctgccg gttcgtcgga catcctgctc ccctctgatg gggcacccta ctgattgacg
     3901 gtggctctgg cgggactccc tcggaggggt accggaccgc tggtcggccg tgcgaagatg
     3961 agggggaggg gagcgggatg gatccagccg agccagtgac cacgatccgg gtgctcttgg
     4021 tcgaggactc gctcgatgac gcggccctgg cggagcgatc cctccgccaa ccgggcttcg
     4081 acttcgaggt cgctcgggtg gagtcggccg ccgcgttccg ctcggcgctg accgagcact
     4141 cgtgggacct catcgtctgc gatcacgggc tgccccagtt cggggtgttc gaggcgatcg
     4201 agat
//
LOCUS       DATMFS010000610         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_324134, whole genome shotgun sequence.
ACCESSION   DATMFS010000610 DATMFS010000000
VERSION     DATMFS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324134"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..497
                     /locus_tag="VNQ33_11740"
     CDS             171..497
                     /locus_tag="VNQ33_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWJ98825.1"
                     /translation="MTVPEIDIAEAARRHAAGTLVIDVREPDEYVEGHVPGAPLIPLG
                     EVTERVDEFPAADEVLIICKLGGRSMKAAEYLRGQGIDAVNIAGGTMAWIDEGHRVVT
                     GDEPGA"
     gene            502..1710
                     /locus_tag="VNQ33_11745"
     CDS             502..1710
                     /locus_tag="VNQ33_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWJ98826.1"
                     /translation="MPWERSDPGPDAADMGDLDGHRWIDDDAAFGSIIDELCGVEAFG
                     IDTEFHRERTYFPQLALIQLSWNDQKALVDPFAVDLAPFAAALQGPATVVMHAASQDL
                     EVLDLACGALPRDLFDTQLAAGFVGHSLPSLAALVERFYGVHLPKGDRLTDWLRRPLG
                     GDQRRYAASDVEYLLGLREKLTAQLEERGRLEWALAECEVLRAKGRIKRKPEDAWLRI
                     KEARTLRGEVAAIARSVAAWREERAMELDQPVRFVFSDLAVVGVAQRKPKTLEELKRI
                     RGVDERHAKGKVGEAVLAAVAAGRGQTVPKPDGPTYDLDRRLRPAVSLVAAWVSQLSR
                     DLEIETSLVATRADIEALLAGDPSARLTVGWRAELVGERIRRLVDGEAALAFDGAGNL
                     VLEDRAPDAG"
     gene            2118..>3026
                     /locus_tag="VNQ33_11750"
     CDS             2118..>3026
                     /locus_tag="VNQ33_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ98827.1"
                     /translation="MSFTALRNVALVAHVDHGKTTLVDALLRSTGVFSAHGAVVDRVM
                     DSNDQERERGITILAKAASVQWGDIKINLVDTPGHADFGGEVERSLELVDGVILLVDA
                     AEGPLPQTRYVLSKALAAGLPAILVLNKVDRQDARPDEVLDEVYELFMDLGADDDDIE
                     FPIISAIARDSKAMVGIGMPGEGDDLTPLLQAIVDTVPAPDVDVDAPLQAIVTNLDAS
                     DYLGRLAIGRVKRGTMRKGERVALLDEEVIEGGKPVERKLGSLMGFTGIGRDDVDELQ
                     AGDLFVVAGFPEVEIGDTIADPATPEA"
BASE COUNT          459 a         1078 c         1064 g          425 t
ORIGIN      
        1 ccgggccgat gccgccttcg gcatcggcgg cgtggcggac gtcgcggcgc ctggcgaggc
       61 gctcgaggac ctggacacgc ccgatgacgt gcgccgttgg cacgatcgcc ggggaccctc
      121 ccgaccgggt gctcccggtc atcgggcctg aacccccgta cactcgcccc gtgaccgttc
      181 ccgagatcga catcgcagaa gccgcccgcc gccacgccgc cggcaccctc gtcatcgacg
      241 tgcgcgagcc cgacgagtac gtcgagggcc acgtccctgg cgcaccgctc atcccgctcg
      301 gcgaggtgac ggagcgggtc gacgagttcc cggcagccga cgaggtcctc atcatctgca
      361 agctcggcgg acgcagcatg aaggccgccg agtacctgag gggccagggc atcgacgccg
      421 tgaacatcgc gggcggcacc atggcgtgga tcgacgaggg ccaccgggtc gtcaccggcg
      481 acgagcccgg cgcctgatcc cgtgccctgg gagcgcagcg atcctggccc cgacgccgcc
      541 gacatgggtg acctcgacgg tcaccgctgg atcgacgacg atgctgcgtt cgggagcatc
      601 atcgacgagc tgtgcggggt cgaggcgttc ggcatcgaca cggagttcca ccgcgagcgc
      661 acctacttcc cgcagctggc gctgatccag ctctcgtgga acgaccagaa ggcgctggtc
      721 gaccccttcg cggtcgacct ggcgccgttc gccgcggcgc tgcagggccc agccaccgtg
      781 gtgatgcacg cggccagcca ggacctggag gtgctggacc tggcctgcgg cgcactcccg
      841 cgggacctgt tcgacacgca gctcgcggcc gggttcgtcg gccactcgct tcccagcctg
      901 gccgcgctcg tcgaacggtt ctacggcgtc cacctgccca agggggaccg cctcaccgac
      961 tggctgcggc gcccgctcgg cggcgaccag cgccggtacg cggcatcgga cgtcgagtac
     1021 ctgctcggcc tccgggagaa gctcaccgcc caactcgagg agcggggtcg gctggagtgg
     1081 gcgctcgccg agtgcgaggt gctgcgggcc aagggccgga tcaagcgcaa gcccgaggac
     1141 gcgtggctgc gcatcaagga ggcccgcacc ctgcgcggcg aggtggcggc gatcgcccga
     1201 tcggtcgccg cgtggcgcga ggagcgggcg atggagctgg accagccggt ccggttcgtg
     1261 ttctccgacc tggccgtcgt cggcgtcgcc cagcgcaagc ccaagacgct ggaggagctg
     1321 aagcggatcc gcggtgtcga cgagcgccac gccaagggca aggtggggga ggccgtgctc
     1381 gccgcggtgg cggcaggacg gggccagacc gtgcccaagc ccgatggccc cacctacgac
     1441 ctggaccggc ggctccgtcc ggcggtctcg ctggtggccg cctgggtgag ccagctctcc
     1501 cgcgacctgg agatcgagac gtcgctcgtg gccacccgtg ccgacatcga ggcgctgctc
     1561 gccggagacc cgagcgcccg cctcaccgtg ggctggcggg ccgaactggt gggcgagcgc
     1621 atccggcgcc tggtcgacgg cgaagccgcg ctggcgttcg acggagcggg gaacctggtc
     1681 ctcgaagacc gcgctcctga cgctggctga cggccgagat cgggacctca caagggttct
     1741 gggccaacgt cggtggtcga gggcggccgc ggtggacatc gtgcgcggtt cgaatgaggt
     1801 ctgggggctt ggcgcggata cacttgccct tcggattacg ccacctcttc gggagtttgg
     1861 cccatgaaaa agggtcgtca ccgccggtac gaagaagctc gggagctgaa gcgctcctca
     1921 gagccgtcgt tcaacgactt gctcgatcga gctgatgaac gctcctgccc ggagtgcggc
     1981 gccgatcctg gtcacgacca cgccggctgg tgcttggcca tgaccgacga agcgcagcag
     2041 gaagcagacg actgggcctg agcccgccgt cacctgcccc ttcgccctcc gctcccaact
     2101 gcaaaacagg ttctcccgtg tccttcaccg cgctccgcaa cgtcgccctc gtggcgcacg
     2161 tcgaccatgg caagaccacc ctggtcgatg cgctcctgcg atccaccggc gtcttcagcg
     2221 cgcacggcgc cgtggtcgac cgggtcatgg actcgaacga ccaggagcgg gaacggggga
     2281 tcacgatcct cgccaaggcg gcgtcggtcc agtggggcga catcaagatc aacctggtcg
     2341 acacgccggg gcacgccgac ttcggcggtg aggtcgagcg ctccctggaa ctggtcgacg
     2401 gcgtcatctt gctcgtcgac gccgccgagg gtccgctgcc ccagacccgg tacgtgctgt
     2461 cgaaggccct ggctgcgggc ttgccggcga tcctcgtgct gaacaaggtc gaccgccaag
     2521 atgcccgccc cgacgaggtg ctcgacgagg tctacgagct gttcatggac ctcggcgccg
     2581 acgacgacga catcgagttc ccgatcatct cggccatcgc ccgggattcc aaggccatgg
     2641 tcggcatcgg catgccgggc gagggcgacg acctcacccc gttgctgcag gccatcgtcg
     2701 acacggtgcc ggcacccgac gtcgacgtcg atgcacccct ccaggccatc gtcaccaacc
     2761 tcgacgcctc cgactacctc ggccgcctgg cgatcggccg cgtcaagcgc ggcacgatgc
     2821 gcaagggcga gcgcgtggcc ctgctcgacg aggaggtcat cgagggcggc aagcccgtcg
     2881 agcgcaagct cggcagcctc atgggcttca ccggcatcgg ccgcgacgac gtcgacgagc
     2941 tccaggccgg cgacctgttc gtggtggcgg ggttccccga ggtcgagatc ggcgacacca
     3001 tcgccgaccc ggcgaccccc gaggcg
//
LOCUS       DATMFS010000611         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_347111, whole genome shotgun sequence.
ACCESSION   DATMFS010000611 DATMFS010000000
VERSION     DATMFS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347111"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2137)
                     /gene="dnaE"
                     /locus_tag="VNQ33_11755"
     CDS             complement(<1..>2137)
                     /gene="dnaE"
                     /locus_tag="VNQ33_11755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWJ98828.1"
                     /translation="SLWIAERADVEIEFGKPLLPNFPLPEGFDDDAAYLKHLTLEGAK
                     KRWGDPLPDSVVERLAYELAVIGNMGFSSYFLITWDLIKHARDAKIRVGPGRGSAAGC
                     AVAYCLWITDLDPIKYDLLFERFLNPDRISMPDIDMDFDSRYRDEMIRYAAERYGRDH
                     VAQIVTFSTIKARAAVRDAARVLGYPYAVGDKVAKAMPPLIMGRDTPLYACLEKHPKF
                     EDGYKMAGDLRQMYETDDDLHRVIDVAKGLEGLRRQDGIHAAAVVITKDPLTDYLPIQ
                     RKPTAGQTIEEAPVVTQYEMGGVEELGLLKMDFLGLRNLDVISDAIDLIKDFRGIDLD
                     IDTISLNDEETLAMLREGNSIGVFQLEGGAMRTLMRSLAPDCFEDVAALIALYRPGPM
                     SVNMHNDYADRKNGRKPVDYFDPAAEELLGDTYGLMIYQESVMRVSQKFAGFTLAEAD
                     NLRKACGKKKPELMAKERDKFERGCTATGYGHLGTPLFDDIEGFANYAFNKSHSFGYG
                     VVTFQTAYLKAHYPVEYLAALLTSVKTNLDKAAVYLNECRTMKIPVLVPDVNRSSSDF
                     VAARIEDGTEVIPFGLSAVRNVGEGLVAKIVEEREANGPFADFYDFCERVDMSVLNKK
                     LLESLIKAGGFDSVGHPRRGLLAVFEQIVDTTIARRKERDMGVMSLFGDVEDVGTSGF
                     SEKAKIPDLHFEKKEQLKFEKEMLGLYVSD"
BASE COUNT          322 a          674 c          751 g          390 t
ORIGIN      
        1 ggtccgacac gtagaggccg agcatctcct tctcgaactt gagctgctcc ttcttctcga
       61 agtgcaggtc cgggatcttg gccttctcgc tgaagccgct ggtgccgacg tcctccacgt
      121 cgccgaagag gctcatcacc cccatgtccc gctccttgcg gcgggcgatc gtggtgtcga
      181 cgatctgctc gaacacggcg agcaggccgc gccgggggtg gccgaccgag tcgaacccgc
      241 cggccttgat gagcgactcc agcagcttct tgttgagcac ggacatgtcg acgcgctcgc
      301 agaagtcgta gaagtcggcg aaggggccgt tggcctcccg ctcctcgacg atcttggcga
      361 cgagcccctc gcccacgttg cgcaccgcgg agaggccgaa ggggatgacc tcggtgccgt
      421 cctcgatccg ggcggccacg aagtccgacg acgaccggtt cacgtcgggc accagcaccg
      481 ggatcttcat cgtgcggcac tcgttgaggt agaccgccgc cttgtccagg ttggtcttga
      541 cgctggtgag cagggcggcc aggtactcga ccgggtagtg cgccttcagg taggcggtct
      601 ggaaggtgac cacgccgtag ccgaacgagt gcgacttgtt gaacgcgtag ttggcgaagc
      661 cctcgatgtc gtcgaacagc ggcgtgccga ggtggccgta gcccgtggcc gtgcagcccc
      721 tctcgaactt gtcgcgctct ttggccatga gctcgggctt cttcttgccg caggccttgc
      781 gcaggttgtc ggcctcggcg agcgtgaagc cggcgaactt ctggctcacc cgcatcaccg
      841 actcctggta gatcatgagc ccgtaggtgt cgcccaggag ctcctcggcg gccgggtcga
      901 agtagtcgac gggcttccgg ccgttcttgc ggtcggcgta gtcgttgtgc atgttcaccg
      961 acatcggccc cgggcggtac agggcgatga gggcggccac gtcctcgaag cagtccggcg
     1021 ccagcgagcg catgagggtg cgcatggcac cgccctcgag ctggaagacg ccgatcgagt
     1081 tgccctcgcg cagcatcgcc agggtctcct cgtcgttgag cgagatggtg tcgatgtcga
     1141 ggtcgatgcc gcggaagtcc ttgatgaggt cgatggcatc ggagatgaca tcgaggttcc
     1201 gcaggcccag gaagtccatc ttgagcaggc cgagctcttc gacgccgccc atctcgtact
     1261 gcgtgacgac cggcgcctcc tcgatcgtct gcccggccgt gggcttgcgc tggatcggca
     1321 ggtagtccgt cagcgggtcc ttggtgatca ccacggcggc ggcgtggatg ccgtcttggc
     1381 ggcgcaggcc ctccaggccc ttggcgacgt cgatgacccg gtggaggtcg tcgtcggtct
     1441 cgtacatctg ccgcaggtcg ccggccatct tgtagccgtc ctcgaacttc gggtgcttct
     1501 cgaggcaggc ataaagcggc gtgtcgcggc ccatgatgag cggcggcatg gccttggcca
     1561 ccttgtcgcc caccgcgtac gggtagccca gcacccgggc ggcgtcgcgc acggcggctc
     1621 gggccttgat ggtggagaag gtcacgatct gggcgacgtg gtcgcggccg tagcgctcgg
     1681 cggcgtagcg gatcatctcg tctcggtagc gggagtcgaa gtccatgtcg atgtcgggca
     1741 tcgagatgcg gtccgggttg aggaaccgct cgaacagcag gtcgtacttg atgggatcga
     1801 ggtcggtgat ccagaggcag taggccaccg cgcacccggc ggccgagcca cggccgggac
     1861 cgacgcggat cttggcgtcg cgggcgtgct tgatgaggtc ccacgtgatg aggaagtacg
     1921 agctgaagcc catgttgccg atgacggcca gctcgtaggc gaggcgctcg acgacggagt
     1981 ccggcagcgg atccccccag cgcttcttgg ccccctccaa ggtgaggtgc ttgaggtagg
     2041 cggcgtcgtc gtcgaagccc tccggcagcg ggaagttggg cagcagcggc ttgccgaact
     2101 cgatctcgac gtcggcgcgc tcggcgatcc acagcga
//
LOCUS       DATMFS010000612         3319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_181187, whole genome shotgun sequence.
ACCESSION   DATMFS010000612 DATMFS010000000
VERSION     DATMFS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181187"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VNQ33_11760"
     CDS             complement(<1..459)
                     /locus_tag="VNQ33_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98829.1"
                     /translation="MTIETAYQDAAQTEGPWFPQLRNDPMKPPQAYEQWREQAAVVPA
                     ELHSEQRAWVVLRHDAVQEVLQHPGISVDAQHPHFPKVRKGVTSRANDTMLRHMDAPM
                     HGKFRRMMTGQFTLKRVNELRPELERIVTEAIDGILAAGGPVDLHKEFSLV"
     gene            complement(571..1893)
                     /locus_tag="VNQ33_11765"
     CDS             complement(571..1893)
                     /locus_tag="VNQ33_11765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ98830.1"
                     /translation="MKRQLHLNTFINAVGHHEAAWRRAEAVPNWNLDIEHYKNLARIA
                     ERGTLDSIFFADIQGLISTPSRRPGEMLDPIVKLTALAEATERIGLIATASTTFDDPY
                     NLARRFASMDIISGGRVGWNVVTSIAKEIGPNFGVTEFPTPEERYERADEFVDVVLKL
                     WDSWEPDALVVDKERGVYADADKVHRIDHVGKHYSVQGPALLPPTPQGRPIIVQAGSS
                     GPGIAFAAKYAEAVFTAQGTIDEGRAFYANLKSQAAALGRNPEHLHVLPGIVTVIGGT
                     EAEAQAKARALDELIVLDYAHAHLAEQTGVPVEELDLDKPLPAHIRPPEEIVDGRSRY
                     EISVGMARRENLTVRELLIRLNGSRGHQKFIGTPEQVADVIEMWFTTGASDGFNVMPA
                     TFPDGLEDFVDHVVPILRQRGLFRSAYEAATLRGHYGLPMPAPAFQPA"
     gene            2172..>3319
                     /gene="dctP"
                     /locus_tag="VNQ33_11770"
     CDS             2172..>3319
                     /gene="dctP"
                     /locus_tag="VNQ33_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWJ98831.1"
                     /translation="MGRKKFFGAVVGTISAALLMAGCSSSAAPAGGNAAAGGLADMEP
                     IKLTVSNIHAETSTAGMFLNDWMEAVKEGSGGKVTFDYYGGGTLHPATEALSAIESGL
                     TQVTFVSTGYWPDQLPVAQWDDKVFQEAVRKFGYPGVNTAGIPAQLAHYGEGSAVAEE
                     FAKHKMIPLLPMTSGPAVLTCAEPFDSPEDLNGRQVRVPNDVSKGENEALGMTGVFLP
                     PNEQYEALQRGVIDCAVNAPTTVLGGGLLEVAKYSAFTENAPSSGSMFVIAKDTWDAF
                     PEPVKELMTKARNEALVKFTQNTLDVYAKYISETEAAGGEIVATEAMDEKLRAWHDAQ
                     PDLKANAPSGVADASAAVERVDGMVKDWVSFTSDELGATSATGLGLDE"
BASE COUNT          548 a         1039 c         1137 g          595 t
ORIGIN      
        1 caccaggctg aactccttgt ggaggtcgac gggtccgccc gcggccagga tgccgtcgat
       61 cgcctcggtg acgatccgct ccagctccgg gcgcagctcg ttgacgcgct tgagggtgaa
      121 ctggcccgtc atcatccggc ggaacttgcc gtgcatcggt gcgtccatgt ggcgcagcat
      181 ggtgtcgttg gcccgcgacg taacgccctt gcgcaccttc gggaagtgcg ggtgctgcgc
      241 gtccacggag atgccggggt gctgcaggac ctcctgcacg gcgtcgtgcc tcagcaccac
      301 ccaggcgcgc tgttcgctgt gcagctccgc cggcacgacc gcggcctgct cacgccactg
      361 ttcgtaggct tgcggcggct tcatggggtc gttgcgcagt tgggggaacc acgggccttc
      421 ggtctgggcc gcatcctggt atgccgtttc gatcgtcatt gatcactacc tccacgctgt
      481 tggatggatc cgccgaagcg gccctgccgg aaagccgtaa aagatcgatg cttcgttccg
      541 cgcgggcccg gcggcgggcc cgcgcggagc ctaggcgggc tggaacgccg gcgcgggcat
      601 gggcaggccg tagtgcccgc gcagggtcgc agcctcgtag gcgctgcgga agagcccgcg
      661 ctggcgcagg atgggcacaa cgtggtccac gaagtcctcc aggccgtcgg ggaacgtcgc
      721 gggcatgacg ttgaagccat cggaagcgcc ggtggtgaac cacatctcga tgacgtccgc
      781 cacctgctcg ggcgtcccga tgaacttctg gtgtccccgg ctgccgttga gccggatcaa
      841 aagctcacgg actgtcaggt tctcccgtcg cgccatgcca acgctgatct cgtagcggct
      901 gcgtccgtcg acgatctcct cgggagggcg gatgtgggcc ggaagcggct tgtcgaggtc
      961 gagctcctcg accggaacgc ctgtctgctc tgccaggtgg gcgtgggcgt agtcgaggac
     1021 gatcagctcg tcgagggccc gcgctttcgc ctgcgcctcc gcttcggtcc cgccgatgac
     1081 cgtcacgatg ccgggaagaa cgtgcaggtg ctcggggttg cgtcccaggg cggccgcctg
     1141 tgacttgagg ttggcgtaga aggcccggcc ctcatcgatc gttccctgcg cggtgaacac
     1201 cgcctcggcg tacttcgccg cgaacgcgat gcccggaccg gaagaacctg cctgcacgat
     1261 gatcggccgt ccctgcggcg tcggggggag aagggccggc ccctgcaccg agtagtgctt
     1321 gcccacgtgg tcgatccggt gcaccttgtc cgcgtcggcg tagacgccgc gctccttgtc
     1381 gacgacgagt gcgtccggct cccagctgtc ccagagcttc agcacgacgt cgacgaattc
     1441 gtcggcgcgc tcgtaccgtt cctccggcgt cgggaattcg gtgacgccga agttggggcc
     1501 gatttccttg gcaatcgatg tcaccacgtt ccagccgacc cgtccgccgc tgatgatgtc
     1561 catggaggcg aaccgccgcg ccaggttgta ggggtcgtcg aaggtggtgg atgccgtggc
     1621 gatcaggccg atgcgttcgg tcgcctcggc gagggcggtc agcttgacaa tggggtcgag
     1681 catttcgccc ggccggcggc tcggggtaga gatgagccct tggatgtcgg cgaagaaaat
     1741 cgagtccagg gtgccgcgct cggcaatgcg cgcaaggttc ttgtagtgct cgatgtccag
     1801 gttccagttg ggcaccgcct cggcgcggcg ccaggcggcc tcatggtgcc ccacggcgtt
     1861 gatgaaggtg ttgaggtgca gttgtctctt catggagggg ctcctgttcg tgtgcacagc
     1921 gtcgactttt gttttgcttt tagtattgca cttataagga ttaaaagtaa gtaggagtgg
     1981 cgggaatggc ggcttttcgg agggggctcg accgtcgccg cccaagcacc tcgaccggcg
     2041 cccgtggcta cacgccccca cttgatctca atctgataac gatacaatca ttattagaat
     2101 taccttgtta gtctcagcgt gggcgccacg gacatgtggc gcgagacaca ttaactgcaa
     2161 gggagatggc agtgggtcgt aagaaattct tcggtgccgt tgtcggcacc atcagcgcag
     2221 cccttctgat ggcgggctgc tcaagctcgg ccgcacctgc cggcggcaac gccgccgcgg
     2281 gcggactcgc cgacatggag ccgatcaagc taaccgtcag caacatccat gccgagacct
     2341 caacggccgg catgttcctc aacgactgga tggaggccgt aaaggagggc agcggcggaa
     2401 aggtcacctt cgactactat ggtggcggca ccctgcatcc ggccaccgag gcgctctcgg
     2461 cgattgaatc gggcctcacg caggtgacgt tcgtcagcac cggatactgg cccgaccagc
     2521 tgcccgtggc gcagtgggat gacaaggtgt tccaggaagc ggtgcgaaag ttcggctatc
     2581 cgggggtcaa taccgcgggc attcccgccc agctcgccca ttacggggaa gggtcggccg
     2641 tggctgagga gttcgccaag cacaagatga tccccctgct tcccatgacg agcgggccgg
     2701 ccgtgctcac ctgtgccgag ccctttgaca gccccgaaga cctgaacggg cggcaggtgc
     2761 gtgtcccgaa cgacgtctcc aagggggaga acgaggcgct gggaatgacc ggcgtgttcc
     2821 ttcccccgaa cgagcagtac gaggcgctgc agcgcggcgt catcgactgc gcggtgaacg
     2881 cccccacgac cgtgctgggc ggcggactgc tcgaggtggc caaatactcc gccttcacgg
     2941 agaacgcccc aagttccgga tcgatgttcg tcatcgccaa ggacacctgg gacgccttcc
     3001 ccgagccggt caaggagctg atgacgaagg ccaggaacga ggcgctcgtc aagttcacgc
     3061 agaacaccct ggacgtgtat gcaaagtaca tttccgagac cgaggcggcc ggaggcgaga
     3121 tcgtcgcgac agaggcgatg gatgagaagc tgcgtgcctg gcacgacgcg cagcctgacc
     3181 tgaaggcgaa cgccccctcg ggggtcgccg atgcctcggc cgcggtcgaa cgggtcgacg
     3241 ggatggtgaa ggactgggtc tcgttcacgt cggatgagct cggggctacc tccgcgaccg
     3301 gcctcggcct ggacgagca
//
LOCUS       DATMFS010000613         5629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_160951, whole genome shotgun sequence.
ACCESSION   DATMFS010000613 DATMFS010000000
VERSION     DATMFS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160951"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VNQ33_11775"
     CDS             <1..527
                     /locus_tag="VNQ33_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98832.1"
                     /translation="NTVSSLAYVAAGADLLRRPEPDRAFAWAVIAVGVGSVAYHGPGG
                     VLGKWMHDSSLIAMFGLLALTDVTTADGREMPDAAVAGVVAAAAVMAHPRTTDYVQGG
                     VGALAVVAEARRHIRQASVREAAVSLPLMTTGLVMHILGRTDERWCRPGSLLQPHAAW
                     HTLSAAALWTRRRF"
     regulatory      695..760
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            912..2282
                     /locus_tag="VNQ33_11780"
     CDS             912..2282
                     /locus_tag="VNQ33_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWJ98833.1"
                     /translation="MTEPQEPARRHTAIGAAVWQIIDFDLREQLDLVRYLVRWCALGS
                     LVGVAAGLSSAFFLWTLDKVTEFRLEHGWLILLLPAAGFVIGFVNHRWGGTSSGGNNL
                     IIDEIHEPRAWIPKRMAALVYIGTEVTHLFGGSAGREGTALQMSGSLTDVISRALHLR
                     EADRRVMLIAALAGGFGAVFGVPIAGCVFGLEVQSVGRLRYDALVPALTAALIGDKVV
                     RGLGVKHAITPELIGYDITAPLLAKVALAGLCFGLTALVFAELTHGIKRIYAAGAVWP
                     PLRPVIGGFAIVGLTLAVGDQAYNGLSLDLAARSLAGGAGIIGAAFALKLLFTAVTLG
                     SGFQGGEVTPLFVIGATLGVTLGRLLGVPTELLAAVGFVSVFAGAANVPLACTVMGAE
                     LFGADGFVLFAVGCVVSYVFSSHRGIYTTQRIAVPKSPAAHLPTEEGGSVGDIARRRR
                     PTAPEH"
     gene            2375..3424
                     /locus_tag="VNQ33_11785"
     CDS             2375..3424
                     /locus_tag="VNQ33_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ98834.1"
                     /translation="MRSRGKIRVGVIGLDHNHGLMLAARLWQAGASITAHHASGAANL
                     VAMRLLGPKARSRPVDAIIDDPDIDLIVTAAIPSERGDIAVRALRSGKHVVADKPGVT
                     TLKGLAAIDQALAETDRRWWVMFGERFENRAVAEACRRARLGDIGRVVSVLGLGPHRM
                     GADGRPDWFWDPVATGGILVDIGSHQADQFLMATGAGTAEVISSQVGNVSVPAHPEMQ
                     DIGQMVVSGGGAIGTHRVDYLSPKGLKTWGDGRLMIVGTEGTLEVRTNVDVAGKKGRE
                     HLILVDAKGTRRIDCATTKVDWARRLVADVVDGSDTFVSADHVRAVSDLTLRAQAEAG
                     SWAASASASAEEAAS"
     gene            3421..4485
                     /locus_tag="VNQ33_11790"
     CDS             3421..4485
                     /locus_tag="VNQ33_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ98835.1"
                     /translation="MSKLRIGVVGLGIGRLHVLALLRLRSHFELVAVADPSPARRRQM
                     RVLGVKGVSDLESLLALGVDVVDICTPPHLHEAQIIQCLEAGADVVCEKPLVDSVAAV
                     DRLAAAEQRTGRRVAPIFQYRWGAGSQKLKRLLDAGIPGRLLVATSETMWKRGDAYYA
                     VDWRGTWKSERGGAVLSHAIHLHDLLTWLGGPPAEVVARTATRTLDIEVEDSAAAIIT
                     TADGALMTATVTLGAATETSRLRFVFENLTVESSQSPYKPGAEPWTFHFASKEVERQA
                     AATLDGWSPPLSGWRGQLAATQEALVSHRPLPVSLDDARTALEVVTAWYRSSRTGQPE
                     SLPLAADHPDRSSWLPEELR"
     gene            4562..5341
                     /locus_tag="VNQ33_11795"
     CDS             4562..5341
                     /locus_tag="VNQ33_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ98836.1"
                     /translation="MPTRDGAPAGAPCWIDLFTDDAPAAHAFYTELFGWTVDVAEEFG
                     GYATFHRDGVAVGGCMGNHQPGHPANFWTVYLRVDDIVATVESAKAAGGGELLAPMPV
                     GDLGQMGMIADPSGAATGLWQVGSFPGIGVLAEPGAPAWFELHTMAYDAVLPFYRDVF
                     GWDIHTASDEPQFRYSTLGKDRDGLAGVMDDAVHAPEGSPSFWNVYIAVADADATVAK
                     AEELGGGVEMKPENTPYGRLAVLTDTTGSRISIMGENTPER"
BASE COUNT          744 a         2112 c         1944 g          829 t
ORIGIN      
        1 cgaacaccgt gtcgagcctc gcgtacgtgg cggcgggcgc ggacctgctc cggcgaccgg
       61 agccggaccg ggccttcgcc tgggcggtca tcgccgtcgg tgtcgggtcg gtggcctacc
      121 acggtccggg cggggtgctc gggaagtgga tgcacgacag ctcgctgatc gccatgttcg
      181 gcctgctggc gctcaccgac gtgacgacgg cggacggtcg ggagatgccc gacgccgctg
      241 tcgccggtgt cgtcgccgct gcggcggtga tggcacaccc ccgcaccacg gactacgtcc
      301 agggcggggt cggggcgctc gccgtcgtgg ccgaggcccg ccggcacatc cgccaggcct
      361 cggtgcgaga ggccgccgtc tcgctgccgc tcatgaccac cggcttggtg atgcacatcc
      421 tcggtcgtac cgacgagcgc tggtgccggc cgggttcgct cctccagccg cacgcggcct
      481 ggcacaccct gtcggcggcg gccctgtgga cccgccgccg cttctgaagg cggcctcgcc
      541 ggcgcacggt gcccgcgatc gggatgggcc cgatcgggcc tggtggaccg gctggcaggc
      601 cgacgagcgt gcccccgacg accgtgagcg tccgacgagt gcgtggatgg acgcggggag
      661 catggtgggt gtcgaagccc ggctggctac gatggaaggc ggaggcgatg gggctcgccc
      721 aaaccgctgg acactcccgg ctgacggtcc ctgcgaacga tcgaaaggcc gatcttcgtg
      781 gtaccgccgt ctccaaaccc agatctccag ccgaccgtcg gagtcgccgt gtcccgtgac
      841 ggcgacctgc tgcaggtcgt ggagctgctg cgtgagcgcg ccgccgaacc ccgccccgag
      901 aagagcgcgc catgaccgaa ccacaggagc cggcccggcg gcacaccgcc atcggagcag
      961 ccgtgtggca gatcatcgac ttcgacctcc gcgagcagct cgacctcgtg cgctacctgg
     1021 ttcgctggtg cgccctcgga agcctcgtcg gcgtcgccgc gggcctgtcg tccgcgttct
     1081 tcctgtggac cctcgacaag gtcaccgagt tccgcctcga gcacggctgg ctgatcctgc
     1141 tgctgcccgc cgccgggttc gtcatcgggt tcgtcaacca ccggtggggc ggtacctcat
     1201 cggggggcaa caacctgatc atcgacgaga tccacgaacc gagggcctgg atccccaagc
     1261 gcatggcggc cctcgtctac atcggtaccg aggtcaccca cctcttcggc ggctccgcag
     1321 gccgtgaggg caccgccctc cagatgtcgg gcagcctcac cgacgtcatc tcccgcgccc
     1381 tgcacctgcg cgaggccgac cgccgcgtca tgctcatcgc cgcgctcgcc ggcgggttcg
     1441 gtgccgtctt cggcgtcccc atcgccggat gcgtgttcgg cctcgaggtc cagtccgtcg
     1501 gccggctccg ctacgacgcc ctcgtccccg ccctcacggc tgcgctcatc ggcgacaagg
     1561 tcgtgcgcgg cctcggggtc aagcacgcca tcacccccga gctcatcggc tacgacatca
     1621 cggctccgct cctcgcgaag gtggccctcg ccggcctctg cttcggcctc accgccctcg
     1681 tgttcgccga gctcacgcac ggcatcaagc gcatctacgc agccggtgcc gtctggccgc
     1741 cgctgcggcc cgtcatcggt ggcttcgcca tcgtcggtct caccctcgcc gtcggcgacc
     1801 aggcctacaa cggcctgtcg ctggatctcg ccgcccggtc gctcgccggc ggtgccggca
     1861 tcatcggcgc agcgttcgcc ctcaagctcc tgttcaccgc cgtgaccctc ggctccggct
     1921 tccaaggcgg cgaggtcacg cccctgttcg tgatcggtgc caccctcggc gtcaccctgg
     1981 gccgcttgct cggcgtcccg accgaactgc tcgccgccgt cgggttcgtg tccgtgttcg
     2041 ccggtgccgc caacgtgccc ctcgcctgca ccgtcatggg cgccgagctc ttcggcgccg
     2101 acgggttcgt gctcttcgcc gtcggctgcg tcgtctccta cgtgttctcc tcccaccgag
     2161 gcatctacac cacccagcgg atcgccgtcc ccaagagccc tgctgcccac ctcccgacgg
     2221 aggaaggggg ctcggtcggc gacatcgccc gccgaaggag gccgacggcg cccgagcact
     2281 agcggcggcc tggcgctggg tcgaagcgga acgcggggaa cggccgccgg cccgcctggc
     2341 tgggccgggt agggggttgg gtagggtcgc ccgcatgcgc tctcggggga agatccgcgt
     2401 cggggtcatc ggtctcgacc acaaccacgg cctcatgctg gccgcacggc tctggcaggc
     2461 gggtgcgtcg atcaccgccc accacgcctc gggcgccgcg aacctcgtgg cgatgaggct
     2521 gctcggcccc aaggcgcggt cccggcccgt cgacgccatc atcgacgatc ccgacatcga
     2581 cctgatcgtc accgcggcca tcccctccga gcgcggcgac atcgcggtcc gggccctgcg
     2641 gtccggcaag cacgtggtgg ccgacaagcc gggcgtcacc acgctgaagg ggctcgccgc
     2701 catcgaccag gcgctggccg agaccgaccg gcgctggtgg gtcatgttcg gcgagcggtt
     2761 cgagaaccgg gcggtggccg aggcgtgccg ccgcgcccgg ctcggcgaca tcggccgtgt
     2821 ggtctccgtg ctgggcctcg gcccccaccg catgggtgcc gacggtcggc ccgactggtt
     2881 ctgggacccg gtcgccacgg gcggcatcct cgtggacatc ggctcgcacc aggccgacca
     2941 gttcctgatg gccaccggtg ccggcaccgc cgaggtcatc agctcccagg tcggcaacgt
     3001 gtcggtgcct gcgcaccccg agatgcaaga catcggccag atggtcgtct ccggcggcgg
     3061 cgccatcggc acgcaccggg tcgactacct gtcgccgaag ggcctcaaga cctggggcga
     3121 cggccgcttg atgatcgtcg ggaccgaggg cacgctcgag gtccggacca acgtcgacgt
     3181 cgccggcaag aagggcaggg agcacttgat cctggtcgat gccaagggca cccgccggat
     3241 cgactgcgcc accaccaagg tcgactgggc tcgccggctc gtggccgacg tggtcgacgg
     3301 cagcgacacc ttcgtgagcg ccgaccacgt gcgggcggtg agcgacctga cgctccgggc
     3361 ccaagccgag gcgggctcgt gggcggcatc agcatcggca tcggccgagg aggcggcatc
     3421 gtgagcaagc tccgcatcgg cgtcgtcggc ctcggcatcg gccggctcca cgtcctcgcc
     3481 ctcctccgcc tgcgctcgca cttcgagctc gtcgccgtgg ccgacccgag cccggcccgc
     3541 cgccgccaga tgcgcgtcct gggcgtgaag ggcgtctcgg acctggagtc gctcctggcg
     3601 ctcggcgtcg acgtcgtcga catctgcacc ccgccgcacc tgcacgaggc ccagatcatc
     3661 cagtgcctgg aggcgggtgc cgacgtggtg tgcgagaagc cgctggtcga ctcggtggcc
     3721 gccgtcgacc gcctcgccgc cgccgagcag cgcaccgggc gccgggtcgc cccgatcttc
     3781 cagtaccggt ggggcgctgg gtcgcagaag ctcaagcgcc tcctcgacgc cggcatcccc
     3841 ggccgcctgc tcgtcgccac cagcgagacc atgtggaagc gcggcgacgc ctactacgcc
     3901 gtcgactggc ggggcacctg gaagagcgag cgcggcggtg ccgtgctcag ccacgccatc
     3961 cacctccacg acctgctcac ctggctcggc ggccccccgg ccgaggtggt ggcccgcacc
     4021 gccacccgca cgctcgacat cgaggtcgag gacagcgcgg cggcgatcat caccaccgcc
     4081 gatggtgcgc tgatgacggc cacggtcacc ctcggggcgg cgacggagac gtcgcgcctc
     4141 cgcttcgtgt tcgagaacct gacggtcgag tcctcgcagt cgccctacaa gccgggtgcc
     4201 gagccgtgga cgttccactt cgcgtcgaag gaggtcgagc ggcaggcggc ggccacgctc
     4261 gacggctggt cgcccccgct cagcggatgg cgcgggcagc tcgccgccac gcaggaggca
     4321 ctcgtctcgc accgcccgct gcccgtcagc ctcgacgatg cccgcaccgc gctcgaggtc
     4381 gtgacggcgt ggtaccgctc ctcgcgcact ggtcagccgg agtcgttgcc gctcgcggcc
     4441 gatcaccccg accggtccag ctggcttccc gaagaacttc gctgagaccg atgaatcggc
     4501 gcgtcggcca ccgtctgatg cggtgaaccc gccatcgaac gacttccaag gagacccgct
     4561 catgcctacc cgtgacggcg cgcccgccgg cgctccctgc tggatcgacc tcttcaccga
     4621 cgatgcgccc gccgcgcacg ccttctacac cgagctgttc ggctggaccg tcgacgttgc
     4681 cgaggagttc ggcggctacg ccacgttcca ccgcgacggc gtggccgtcg gcggctgcat
     4741 gggcaaccac cagcccggtc atccggcgaa cttctggacc gtgtacctgc gggtcgacga
     4801 catcgtcgcc acggtcgagt ccgccaaggc ggcgggcgga ggcgagctct tggcgccgat
     4861 gccggtgggc gatctcggcc agatggggat gatcgccgat ccgagcggtg cggccaccgg
     4921 gctctggcag gtgggctcgt tccccggcat cggcgtgctg gccgaaccgg gcgcgccggc
     4981 gtggttcgag ctccacacga tggcctacga cgcggtgctg cccttctacc gggacgtgtt
     5041 cgggtgggac atccacaccg cgagcgacga gccgcagttc cgctacagca ccctcggcaa
     5101 ggaccgggac gggctcgccg gcgtgatgga tgacgcggtg cacgcccccg agggctcgcc
     5161 gtcgttctgg aacgtctaca tcgccgtcgc cgatgccgac gccaccgtcg ccaaggccga
     5221 ggagctgggt ggcggcgtcg agatgaagcc cgagaacacc ccctacggcc gcctcgccgt
     5281 cctcaccgac accactggct cgcgcatcag catcatgggc gagaacaccc cggagcgatg
     5341 aggtgggagc accgcgagcg cacggctcgc ggtgctcgcg ccgaccgcac cgggctcggc
     5401 cagagacgga tctctgggtg tcctcccgct cacgctcgtc gcgctgatcc gggccgtccg
     5461 cccggagcgc atcgccgccc tcgagggtcc cgtgcccgag gtggccctcg aggccaccgg
     5521 gaccgttgtg agtccgggcg cggcgcgcgt ggcagcatcg gctccgtgaa cggtggaacg
     5581 acgacccctg gctggtaccc ggacccttgg tacccggggg gtgtgcggt
//
LOCUS       DATMFS010000614         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_181266, whole genome shotgun sequence.
ACCESSION   DATMFS010000614 DATMFS010000000
VERSION     DATMFS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181266"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VNQ33_11800"
     CDS             <1..1141
                     /locus_tag="VNQ33_11800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ98837.1"
                     /translation="EVELPGTPFPPSSNYVRYEPHGVTTGIIPWNFPFIMAAWKLGPA
                     LAAGNCAVLKPASFTSITALMLGEVFQEVDLPPGVANIVAGPGGTAGEELAVNPLVDK
                     TAFTGSTEVGRRIMQLASGTVKPVTLELGGKSANIVLDDADLDLAAACVLWGTFFHNG
                     QVCESGTRALVHKSIYDDFLSLLVDRAGKIVIGNQMDFETDLGPLVSRSQVETVERYV
                     QLGRDEVGDPVCGGQRPEGLADGLDPAAFYTPTIFAGVPNSAKIAQEEIFGPVLSVIP
                     FETDDEAVAIANDSIYGLAGGVQSTNPERAKSVANRMRTGTVWINDYHLISPERPFGG
                     YKQSGIGRELGINGLRAYQQVKHVHINPGGTRDNYIHLSALSGSI"
     gene            complement(1236..1577)
                     /locus_tag="VNQ33_11805"
     CDS             complement(1236..1577)
                     /locus_tag="VNQ33_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98838.1"
                     /translation="MKLRILAVATLALALPLLATGCGGSDDTNSGERPTADEISKSFQ
                     KQLPPTTPNVEAVADCLGKELEASELPNGVLRSLVAGEEETKVDKGNEEKYTKEITSA
                     TTACMSEALGS"
     gene            1718..2140
                     /locus_tag="VNQ33_11810"
     CDS             1718..2140
                     /locus_tag="VNQ33_11810"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HWJ98839.1"
                     /translation="MLVTATDRARDVIAEMGHRRRGTLTVTIGTGCCESTAPFLYEDF
                     WPGPDQEPVGEVEGVVVYAPEYLRSAYPGDDGVVLDVVDELAESLSVETELGCRLILR
                     GLGFTSADEPPVCEVPGTLSRGVVGEFPEALRGIRIHG"
     gene            complement(2177..>2530)
                     /locus_tag="VNQ33_11815"
     CDS             complement(2177..>2530)
                     /locus_tag="VNQ33_11815"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWJ98840.1"
                     /translation="KSFLRVAGVVENMSAFTDHHGETHALFGSGGGQTLADDAGVPLL
                     GQVPIEPLVSAGGDAGEPEVLGDSPAAQAFRDIAERLVTEAVPPAELAGCSARMLELA
                     VAALDAEDEREANAV"
BASE COUNT          364 a          915 c          871 g          380 t
ORIGIN      
        1 cgaggtcgag ctccccggca cgccgttccc gccgtcgtcg aactacgtcc gctacgagcc
       61 ccacggcgtc accaccggga tcatcccgtg gaacttcccg ttcatcatgg cggcgtggaa
      121 gctcggcccc gccctggccg ccggcaactg cgccgtgctg aagccggcgt cgttcacctc
      181 gatcaccgcc ttgatgctgg gcgaggtgtt ccaggaggtc gacctcccgc cgggcgtcgc
      241 caacatcgtc gccggcccgg gtggcaccgc cggtgaggag ctggccgtca acccgctggt
      301 cgacaagacg gcgttcaccg gctccactga ggtcggccgc cgcatcatgc agctcgcgtc
      361 ggggaccgtg aagccggtga ccctcgagct cggcggcaag agcgccaaca tcgtgctcga
      421 cgacgccgac ttggacctcg ccgccgcctg cgtcctgtgg ggcaccttct tccacaacgg
      481 ccaggtgtgc gagtccggca cccgggccct cgtgcacaag agcatctacg acgacttcct
      541 gtcgctgctg gtcgaccgcg ccggcaagat cgtcatcggc aaccagatgg acttcgagac
      601 cgatctcggc ccgctcgtga gccgcagcca ggtcgagacg gtcgagcgct acgtgcagct
      661 cggccgcgac gaggtcggcg acccggtctg cggcggtcag cgccccgagg gcctcgccga
      721 cggcctcgac ccggctgcct tctacacgcc aacgatcttc gccggggtcc cgaactccgc
      781 caagatcgcc caggaggaga tcttcggacc ggtgctgtcg gtcatcccgt tcgagaccga
      841 cgacgaggcc gtcgccatcg ccaacgactc gatctacggc ctcgccggcg gcgtgcagtc
      901 caccaacccg gagcgggcca agtcggtggc caaccgcatg cgcaccggca cggtgtggat
      961 caacgactac cacctgatca gccccgagcg tcccttcggt ggctacaagc agtcgggcat
     1021 cggccgggag ctggggatca acggcctccg cgcctaccag caggtgaagc acgtccacat
     1081 caaccccggt gggacgcgcg acaactacat ccacctctcg gcgctgagcg gcagcatcta
     1141 gcccgcaccg gccccagcgg aacgcacagc gccccgggtc cgaggacccg gggcgctgtc
     1201 gcgagctcgg tgagccgagg cggaacctgg cgggcctagc tcccgagggc ctcgctcatg
     1261 caggccgtcg tggcgctggt gatctccttc gtgtacttct cctcgttgcc cttgtcgacc
     1321 ttcgtctcct cctcgccggc gacgagggag cgcaagacgc cgttggggag ctccgaggcc
     1381 tcgagctcct tgccgaggca gtcggcgacg gcctcgacgt tgggcgtggt cggcgggagc
     1441 tgcttctgga aggacttgga gatctcgtcg gccgtcgggc gctcgcccga gttggtgtcg
     1501 tcgctgccgc cgcagccggt ggccagcagg ggcagcgcca gggcgagggt cgcgacggcg
     1561 aggatgcgca gcttcatggg gggactcctt gggtggatcg gatcgaagcg ccacgtccag
     1621 gagcacccgc caggagtccc gctcttcgtg gcggccccaa cgctactcag gacaggtgtc
     1681 caggtgcacg gatcgcggat ccccgtaggc tggccgcatg ctcgtgaccg ccaccgaccg
     1741 cgcccgcgac gtcatcgccg agatgggcca ccggcggcgc ggcacgctca ccgtcaccat
     1801 cggcacgggt tgctgcgaga gcacggcacc gttcctctac gaggacttct ggccggggcc
     1861 ggaccaagaa ccggtgggcg aggtggaagg cgtggtggtc tacgcacccg agtacctccg
     1921 ctcggcctac cccggcgacg acggcgtcgt gctcgacgtg gtcgacgagc tggccgagtc
     1981 gctgtcggtc gagaccgagc tgggctgccg cctcatcctc cgcgggctgg gcttcacctc
     2041 cgctgacgag cccccggtgt gcgaggtccc gggcaccttg tcccgcggcg tcgtgggcga
     2101 gttccccgaa gccctccgag gcatccgcat ccacggctga cgctttcggc tccggccgcc
     2161 gcgctgctcg ctgacctcag acggcgtttg cctcgcgctc gtcttcggca tcgagggcgg
     2221 cgacggccag ctccagcatc cgggcggagc agccggcgag ctcggccggc ggcacggcct
     2281 cggtcacgag tcgttccgcg atgtcgcgga aggcctgggc ggcgggcgag tcacccagca
     2341 cctcgggctc accggcatcg ccaccggccg agacgagggg ctcgatgggc acctggccga
     2401 gcagcgggac gccggcatcg tcggcgaggg tctggccgcc gcctgagccg aacagggcgt
     2461 gggtctcgcc gtggtggtcg gtgaaggcgc tcatgttctc gacgacaccg gccacccgca
     2521 ggaagctctt
//
LOCUS       DATMFS010000615         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_251890, whole genome shotgun sequence.
ACCESSION   DATMFS010000615 DATMFS010000000
VERSION     DATMFS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251890"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1530)
                     /locus_tag="VNQ33_11820"
     CDS             complement(13..1530)
                     /locus_tag="VNQ33_11820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ98841.1"
                     /translation="MAPDAERPAPTAPSDDDIGSSAAAAAATGEAIETAETAGPIEAA
                     SAADAARRPWPFGAWLAVSAVTGLGVRLAYTIGWRIDAGLQYDGPVYVARARFLREGL
                     AFKNPDAWFFSQISSEGAVHPPGNALLIAAAQQIGLRSAAQIQLLGCAVGVTTIVVIG
                     LLGREVAGRRVGIVAAAIAAVSPGLWSYDPTAMAESPGQLLTAILLLLAYRFWQTPTV
                     RRAAYLGGLAGLAALTRSELLILVAVFVLPLCFIARGTTRQALTRVGAATLWTLMVIG
                     PWLGWNLVRFEHPVTMASGIDLSLAYAQCDDTWYGTNTGYWNLFCATNVTEAPENVLA
                     DESELGTQYRSQAGRYIADHRSRWPIVIAARAGRTLSVYPPNAQITVEAERESRERPV
                     LWAATLATWCTYLLAAAAFARPPRSRRHLLPLLAPLVAGIAGATITFGTSRYRSAGEV
                     GLIVLAAVGIDAIGRTWNARAPERGSPADLTAADGPPPHATAGPNAAIMGAAPTT"
     gene            1616..2809
                     /locus_tag="VNQ33_11825"
     CDS             1616..2809
                     /locus_tag="VNQ33_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98842.1"
                     /translation="MDDGPGTAPASVGHVLPLATVAVGALLAFRLGLLAGGLVAVDVV
                     LALLGWWLASAAVAGGSLRSRSTDAWRRAWPPAIGALVLAWAWVLVTDSTRYDTRVRG
                     EALGLVAGSGNWQLLLVGPVERSASRGASALQPLWLVAVAVQLALVLALAWWATRPRA
                     RRHPGTRDPLVVVALALAVAALVVVAVQVVAGAGGQTMLLSTWSRAAAFFLGAALGAL
                     RPGPWTSELRAVVLGTRWIAAAVLVVLAVLAAPDSEWWTSGGVLVLPLVAAIVVAAAV
                     PWPPGRRSRAAPSDIDPWLVLVSVWFLHGPAFALARSLVADAPAVAAGVLGLLVLAVA
                     TLLVVLVSSRLTWTPEAVERRCVLVPPVAVLLIVLLFSATGAFHWSGPAPRSDVSVAP
                     SVPPR"
BASE COUNT          307 a         1015 c         1158 g          435 t
ORIGIN      
        1 ctcgttcctg cgtcacgtcg tcggtgcggc gcccatgatg gccgcgttcg gtccagcggt
       61 ggcatgcggt ggtggaccgt cggcggccgt gagatccgcc ggagaccctc gctccggggc
      121 gcgagcgttc cacgtcctcc cgatcgcgtc aatgccgacg gcggcgagca cgatgaggcc
      181 cacctcgccg gcggagcggt accgcgaggt accgaaggtg atcgtggcgc cggcgatgcc
      241 agcgacgagc ggagcgagca gcggaaggag gtggcggcgc gaccggggcg gccgggcgaa
      301 cgcggccgcg gccagcagat aggtgcacca ggtggcgagc gtcgccgccc acaagacggg
      361 ccgctcgcgg ctctcccgtt cggcctcgac ggtgatctgc gcgttgggcg ggtagacgct
      421 cagggtcctc ccggcgcggg cggcgatcac gatcggccat cggctccggt gatcggcgat
      481 gtagcgaccg gcctggctcc ggtactgggt tccgagctcg gactcgtcgg cgaggacgtt
      541 ctccggggcc tcggtgacgt tggtggcgca gaacaggttc cagtagccgg tgttggtgcc
      601 gtaccaggtg tcgtcgcact gggcgtaggc gagcgacagg tcgatgccgc tcgccatcgt
      661 gacggggtgc tcgaagcgga ccaggttcca cccgagccag ggcccgatca ccatcagggt
      721 ccagagcgtg gcggccccca cgcgggtcag ggcctggcgg gtggtgccgc gggcgatgaa
      781 gcacagcggg aggacgaaga cggcgacgag gatgagcagc tcgctgcggg tcagggcggc
      841 cagaccggcg aggcccccga ggtaggcggc acgccgcacc gtcggcgtct gccagaagcg
      901 gtaggcgagc agcagcagga tcgccgtgag gagctgaccc ggggactcgg ccatcgccgt
      961 ggggtcgtag gaccacaggc cggggctcac cgctgcgatc gcggcggcca cgatcccgac
     1021 gcgccgcccc gccacctcgc gcccgagcag gccgatcacc acgatggtgg tcacgccgac
     1081 ggcgcagccc aggagctgga tctgcgcggc gctgcggagt ccgatctgtt gggcggcggc
     1141 gatcaacagc gcgttgcccg gcgggtgcac cgctccctcg gaggagatct gggagaagaa
     1201 ccaggcgtcg gggttcttga aggcgaggcc ctctcgcagg aagcgggcgc gcgccacgta
     1261 gacggggccg tcgtactgga gcccggcgtc gatccgccag ccgatcgtgt aggcgaggcg
     1321 gacgccgagg ccggtgacgg ccgacaccgc caaccaggcg ccgaacggcc acggcctccg
     1381 ggccgcgtcg gccgctgaag ccgcctcgat ggggccggcc gtctcggccg tctcgatggc
     1441 ttcgccggtc gccgcggcag cggcggcgga cgacccgatg tcgtcgtcgg agggcgcggt
     1501 gggggcgggg cgctcggcgt cgggggccat ggtggcgcca agactgtcac ctgcgcgctg
     1561 gcgaggggcg cacggcatcg gcctccagca gcggcggatc ggtaggatcg gcgccgtgga
     1621 cgacggaccg gggaccgctc cggcttcggt gggtcacgtg ctccccctcg ccacggtggc
     1681 cgtcggcgcc ctgctggcgt tccggctcgg gctgctggcc ggtggcctgg tcgcggtcga
     1741 cgtcgtgctg gcgctgctcg ggtggtggct cgcctcggcg gcggtggccg gtgggtccct
     1801 gcggtctcgg tccaccgacg cgtggcgccg ggcttggccc ccggcgatcg gtgctctggt
     1861 cttggcctgg gcgtgggtgc tcgtgaccga ctcgacccgc tacgacacgc gggtgcgggg
     1921 cgaggcgctc gggctggtgg cgggctccgg caactggcag ctcctgctgg tcgggcccgt
     1981 cgagcggtct gcctcccggg gggcctcggc gctccagccg ctctggctcg tcgcggtcgc
     2041 cgtgcagctg gccctcgtct tggccttggc ctggtgggcc acgcggccgc gggcgcgtcg
     2101 gcaccccggc acccgggacc cgttggtggt ggtggcactc gcgttggccg tggccgccct
     2161 cgtcgtggtc gcggtgcagg tggtcgcggg cgcgggcggc cagacgatgc tgctgtccac
     2221 ctggtcgcgc gctgccgcct tcttcctcgg cgcggcgctc ggcgccctgc gcccggggcc
     2281 gtggacgagc gagctgcggg cggtcgtgct cggcacccgg tggatcgccg ccgcggtcct
     2341 cgtcgtgctc gcggtgctcg cagccccgga ctcggagtgg tggacctccg ggggcgtgct
     2401 cgtgctcccg ctggtggccg cgatcgtcgt cgccgccgcc gtgccctggc caccgggccg
     2461 gcgctcgagg gcggctcctt cggacatcga cccgtggctc gtgctcgtgt cggtctggtt
     2521 cctgcacgga ccggcgttcg cgctggcgag gtcgctcgtc gccgatgccc cggccgtggc
     2581 cgctggcgtg ctcggtctgc tcgtgctcgc cgtcgccacc ctgctcgtgg tgctggtgtc
     2641 gtcccggctc acgtggacgc ccgaggcggt cgagcggcgc tgcgtgctgg tccccccggt
     2701 ggccgtcttg ttgatcgtgc tgctcttctc ggcgaccggc gcgttccact ggtccgggcc
     2761 cgcgccgcgg tccgacgtgt ccgtcgcacc gtccgtgccg ccgcgttgac ggcgctgcgc
     2821 cgaccttccg gtcgaacgag tcctacgatc ggcgagacgt ccggcgaccg cccgagccgt
     2881 tggggcggcc tcgcatgaac gcagacacca ccacg
//
LOCUS       DATMFS010000616         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21520, whole genome shotgun sequence.
ACCESSION   DATMFS010000616 DATMFS010000000
VERSION     DATMFS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21520"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VNQ33_11830"
     CDS             <1..1120
                     /locus_tag="VNQ33_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWJ98843.1"
                     /translation="GAAAVVISDAGSPTERAADVDGYASALAKVDGVTRVDAETGIYC
                     GSSGTLGGAACEPGALVVPAAASPDLTARFSQPDATYLSVVPSVEPLSPEGEAFASDL
                     RQVASPFEEVEIGGQSAQLVDAKSSIFGDVPLALAIIAVITFVLLFLMFGSVVVPIKA
                     LALNILSLSATFGAMVFVFQEGHGSGLLGFTPTGTIAATIPVLMFCVAFGLSMDYEVF
                     LLSRIKEEHDKGSDNITSVARGLARTGGIVTAAALLISVVFLAFATSEVSFIKLFGIG
                     LTLAVLLDAFIIRGTLVPAFMRLAGDANWWAPAWMRRIHARIGISEHVELDDPDEAGP
                     TQLGADADVDVDVDLTASGPPAADGGPTDVGEGDKLTV"
     gene            1123..1746
                     /locus_tag="VNQ33_11835"
     CDS             1123..1746
                     /locus_tag="VNQ33_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ98844.1"
                     /translation="MAPRARAKRGEGERTAEAILDAADALLVGSGSEDAVAIRGVADA
                     VGLTPAAIYRHFPDKETLLFAVCARHFDAITADVAVPALESADDPIDALRRLADAYVR
                     FGVENPEHYRIMFMRRQDHRPEQYAAEQVLETGLFGVVLPVVQQGIDEGRFRPELTDP
                     LLIAWVLWMAVHGITSAAVAMPNFPAPPLDVQLQATVDAVLLGLLAD"
     gene            complement(1849..>2040)
                     /locus_tag="VNQ33_11840"
     CDS             complement(1849..>2040)
                     /locus_tag="VNQ33_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWJ98845.1"
                     /translation="IDDVGRPIEVGNGHATIGASVGLAIGRPGEAPTSVLDAADGALL
                     SAKANGRGCWTVASPRPPR"
BASE COUNT          262 a          746 c          716 g          316 t
ORIGIN      
        1 cggtgccgcc gccgtcgtca tctcagacgc cggctcgccc acggagcggg ctgccgacgt
       61 cgacgggtac gcctcggccc tcgccaaggt cgacggcgtg acacgggtcg atgccgagac
      121 ggggatctac tgcggctcgt ccgggacgct cggcggtgcc gcgtgcgaac cgggcgcgct
      181 cgtggtgccg gccgcggcca gcccggacct cacggcccgg ttcagccagc ccgacgccac
      241 ctacctctcg gtggtgccca gcgtcgagcc gctctcgccc gagggcgagg cgttcgcctc
      301 cgacctccgg caggtcgcca gccccttcga agaggtcgag atcggcgggc agtcggcgca
      361 gctggtcgac gccaagagct cgatcttcgg cgacgtgccc ctcgccttgg cgatcatcgc
      421 cgtcatcacg ttcgtgctcc tgttcttgat gttcgggagc gtggtcgtgc cgatcaaggc
      481 gctggcgctc aacatcttga gcctgtccgc cacgttcggg gccatggtct tcgtcttcca
      541 ggagggccat ggatccggac tgctcggctt caccccgacg gggaccatcg ccgccaccat
      601 cccggtgctg atgttctgcg tcgccttcgg gctgagcatg gactacgagg tcttcttgct
      661 ctcccggatc aaagaggagc acgacaaggg gtccgataac atcacgtcgg tggcgcgcgg
      721 cctggcgcgc accggcggca tcgtcaccgc ggcggcgctg ctcatctcgg tggtgttcct
      781 cgccttcgcc acgtccgagg tgagcttcat caagctgttc ggcatcggcc tgaccctcgc
      841 cgtgctgctc gacgccttca tcatccgcgg cacgctcgtc ccggcgttca tgcgcctggc
      901 cggcgacgcc aactggtggg caccggcgtg gatgcggcgg atccacgccc gcatcggcat
      961 cagcgagcac gtcgagctcg acgatccgga cgaggcgggt cccacccagc tcggggccga
     1021 tgccgacgtc gacgtcgacg tcgacctgac ggcctcgggg cctccggctg cggacggggg
     1081 ccccacggac gtcggcgagg gtgacaagct caccgtgtga aggtggcccc ccgagcacga
     1141 gccaagcgcg gcgaaggcga gcggaccgcg gaggcgatcc tcgacgcggc cgacgccctc
     1201 ctggtgggat cgggctccga ggacgcggtc gccatccgcg gcgtggctga cgccgtgggc
     1261 ctcacgccgg ctgccatcta ccggcacttc cccgacaagg agacgctcct gttcgcggtg
     1321 tgcgcccggc acttcgacgc gatcaccgcc gatgtggcgg tccccgccct ggagtcggcc
     1381 gacgacccca tcgacgcgct gcgccggctg gccgatgcct acgtgcgctt cggcgtggag
     1441 aaccccgagc actaccggat catgttcatg cggcgccagg accaccggcc cgagcagtac
     1501 gccgccgagc aggtgctcga gaccggactg ttcggggtcg tgctcccggt ggtgcagcag
     1561 gggatcgacg agggccgctt ccgcccggag ctgaccgatc cgctgctcat cgcctgggtg
     1621 ctgtggatgg cggtccacgg catcacctcg gccgcggtgg cgatgccgaa cttccccgcc
     1681 ccacccctcg acgtccagct ccaggccacg gtcgacgccg tcctcctcgg cctcctcgcc
     1741 gactgaggcc gccctggccg acccggaccg gacttcggac cggatgctgt cgccttcgac
     1801 ggcggccggt acgaggccgc gccggtgcga ccgatgaggg cgcggccgtc agcggggagg
     1861 gcgggggctc gcgacggtcc agcagcctcg gccgttcgcc ttggcgctga gcagggcgcc
     1921 gtctgcggcg tcgaggacgc tggtcggcgc ctcgccgggg cggccgatgg ccaagccgac
     1981 cgaggcgccg atcgtcgcat ggccgttgcc gacctcgatg ggcctgccga cgtcgtcgat
//
LOCUS       DATMFS010000617         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_67262, whole genome shotgun sequence.
ACCESSION   DATMFS010000617 DATMFS010000000
VERSION     DATMFS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67262"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..694)
                     /locus_tag="VNQ33_11845"
     CDS             complement(221..694)
                     /locus_tag="VNQ33_11845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ98846.1"
                     /translation="MQDSVTVHMDATPAEVWALVSDVTRIGEFSPETFEAEWLDGATG
                     PEVGARFRGHVKRNEKGPVYWTTCEVLVCEPEQEFTFGVLTNGQLLNTWGYRLEAVEG
                     VEGRGTDVTEFFRLTPTLALKVYWTLLGWARGKTNRNGMQRTLEAMKAVVEADAG"
     gene            782..1987
                     /locus_tag="VNQ33_11850"
     CDS             782..1987
                     /locus_tag="VNQ33_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98847.1"
                     /translation="MTTDPGLLVKERIEQLLDTVDPKAVSMEELRGQQYDLGLAWVHF
                     PEGWGGLGLAPNHQKDIDAALRRAGAQPADSRHFFGLTMAGPTVVTHGDDALRGRLLR
                     RMFTGEDAWCQLFSEPGAGSDLAGLACKAVRDGDEWIITGQKVWNTLAHIADRGMLVA
                     RTDPDAPKHKGMTYFALDMHAPGVEVRPLRQITGEAEFNEVYMTEVRVPDSDRIGDVG
                     EGWRVAMTTLSNERTTIGGGGGGVPARGTGAIAEAVRIWNEEPNVDKSAAARDELMKL
                     WIRAEVLRLTNIRATQNRRAGNPGPEGSIAKLVFAQVNMDVYEFCVDLLGPAGLIDYE
                     YIDKRVENLGLTGPPGSSRKMFLRARANSIEGGTSEIQRNIIGERVLGLPGEPRTDKD
                     VPWKDVPRS"
     gene            1984..2514
                     /locus_tag="VNQ33_11855"
     CDS             1984..2514
                     /locus_tag="VNQ33_11855"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ98848.1"
                     /translation="MSERSEVRVRLAGAGDVEVLPVIEAAAGERFRTIPGLELVADHD
                     PSLPPDVLSRALAEGRVWVAEARDELVGYALALDLDGQPHLEQVSVLPEWSGRGVGRE
                     LIDAVASWAKGRASSVTLATYRDVPWNGPLYAHLGWSVLPEEEVAADPRLLAVRAEEA
                     DHGLDPDARVFMRLRV"
     gene            2684..>3225
                     /locus_tag="VNQ33_11860"
     CDS             2684..>3225
                     /locus_tag="VNQ33_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98849.1"
                     /translation="MKIRMVVVVLLASLLVGLNPVAGGSAPGRERIAPPGVASAPRIW
                     YSDFGTGVDGMAIDPGTGRAFVREGATFGGADLVVAGPDTYDAPVKLTGPYGYPVASA
                     GSIFVFRDGMLRRIDPASLSETGSWTVPGLSTDLWVTSVGGDIVWVTRSSEAPFDEQL
                     YRFDPVTGTTTTSGPTNLGYA"
BASE COUNT          460 a         1118 c         1180 g          467 t
ORIGIN      
        1 gtggcgacgt cgaactcgtg gagcgtgtcg gtggtggggg aggcatcgac catcgcgcca
       61 ccctaggtcc gcagttggtt gcatgaaaga accaagtcgg tgccatgggg cacagccggt
      121 ccgcccaagc cccagcccca gccccaagcg gggggaactt cgtatgcgag agcgccgaga
      181 tgggccttgc ggcacacgaa gttcagggca gtggcgtgga tcaaccggcg tcggcctcga
      241 cgacggcctt catggcttcg agggtgcgct gcatgccgtt gcggttggtc ttgccgcggg
      301 cccagccgag gagggtccag tagaccttga gcgcgagggt gggcgtgagc cggaagaact
      361 cggtcacgtc ggtgcctcgg ccctcgacgc cctcgaccgc ttcgaggcgg tagccccacg
      421 tgttgagcag ctggccgttc gtcagcacgc cgaacgtgaa ctcctgctcg ggctcgcaga
      481 ccaggacctc gcaggtggtc cagtagaccg gccccttctc gttgcgcttc acgtggccgc
      541 ggaagcgggc gccgacctcg gggccggtgg cgccgtcgag ccactcggcc tcgaacgtct
      601 cggggctgaa ctcgccgatg cgggtcacgt cgctgaccag cgcccacacc tcggcgggcg
      661 tcgcatccat gtgcacggtc acggagtcct gcatggtcgg gaccctacga ccgctccgag
      721 cgccgcgccg agggagccgg tacgttcgac atcccgcccg tctcgtcccc cggaggcacc
      781 gatgaccacc gatcccggcc tgctcgtcaa ggagcgcatc gagcagctgc tcgacaccgt
      841 cgaccccaag gcggtgtcga tggaggagct ccgcggccag cagtacgacc tgggactggc
      901 gtgggtgcac ttccccgagg gctggggtgg cctcggcctg gcgccgaacc accagaagga
      961 catcgacgcc gcgctgcggc gggccggtgc gcagccggcg gactcccgcc acttcttcgg
     1021 cctgacgatg gccggaccca cggtcgtgac ccacggcgac gacgccctgc gcggccggct
     1081 gctgcgccgg atgttcaccg gcgaggacgc ctggtgccag ctcttcagcg agccgggtgc
     1141 gggctccgac ctcgccgggc tggcgtgcaa ggcggtgcgc gacggcgacg agtggatcat
     1201 caccggccag aaggtgtgga acaccctggc ccacatcgcc gaccgcggca tgctcgtcgc
     1261 ccgcaccgac cccgatgcgc ccaagcacaa gggcatgacc tacttcgccc tcgacatgca
     1321 cgcgcccggc gtcgaggtgc gcccgctgcg ccagatcacc ggcgaggccg agttcaacga
     1381 ggtgtacatg accgaggtcc gggtcccgga ctcggaccgc atcggcgacg tgggcgaggg
     1441 ctggcgcgtg gccatgacca ccctgtcgaa cgagcgcacc accatcggcg gtggcggcgg
     1501 cggtgtgccc gctcgtggca ccggtgccat cgccgaggcg gtgcgcatct ggaacgagga
     1561 gccgaacgtc gacaagtcgg cggccgcccg cgacgagctg atgaagctgt ggatccgcgc
     1621 cgaggtcctg cgcctcacca acatccgcgc cacccagaac cgccgggccg gcaaccccgg
     1681 tcccgagggc tcgatcgcca agctcgtgtt cgcccaggtg aacatggacg tctacgagtt
     1741 ctgcgtggac ctgctcgggc cggccgggct gatcgactac gagtacatcg acaagcgggt
     1801 cgagaacctg ggcctcaccg ggccgcccgg gagcagccgc aagatgttcc tgcgggcccg
     1861 agccaactcg atcgaaggcg gcacgtcgga gatccagcgc aacatcatcg gcgagcgggt
     1921 gctcggcctc cccggcgagc cccgcaccga caaggacgtc ccctggaagg acgtgccgcg
     1981 cagttgagcg agcgttcgga ggtgcgggtc cggctcgccg gtgccggcga tgtcgaggtg
     2041 cttccggtga tcgaggcggc ggccggcgag cggttccgga cgatccccgg cctcgagctc
     2101 gtggccgacc acgacccgtc gctgccgccc gacgtccttt ctcgggcgtt ggccgaaggc
     2161 cgggtctggg tcgccgaagc aagagatgag ctggtcggct acgcgctcgc cctcgacctc
     2221 gacggccagc cccacctcga gcaggtctcc gtgctgccgg agtggtccgg ccgcggcgtc
     2281 ggccgggagc tcatcgacgc ggtggcgtcg tgggcgaagg gccgggcgtc gtcggtgacc
     2341 ctggcgacct accgagacgt gccctggaac ggccccctct acgcccacct cggctggtcg
     2401 gtcctgcccg aggaggaggt ggccgcggat ccccggttgc tcgccgtccg cgccgaggag
     2461 gccgaccacg gcctcgaccc cgacgcgcgg gtcttcatgc gcctgcgggt ctgaccagcg
     2521 agcgggtcgg tgccgtcgac ggctgcggga gccccgcacg cggagggctt ggtccgcggc
     2581 gtcggccttc ggcggggtgg agtcgagggc acggtccgtc cgctcaagcg gagtgggtat
     2641 cgtgccgatg gggcaagacc gatcgtgaac aggggatgac ggcgtgaaga tccggatggt
     2701 cgtcgtggtg ctgctggcgt cgctgctggt ggggctgaac cccgtggccg gaggctcggc
     2761 gcccggtcgg gagcgcatcg ccccacctgg ggtcgcgtcg gccccccgca tctggtactc
     2821 ggacttcggc accggcgtgg acggcatggc gatcgatccg ggcaccgggc gggcgttcgt
     2881 gcgggaggga gcgacgttcg gtggggcgga cctggtggtg gctgggcccg acacgtacga
     2941 cgcaccggtc aagctgaccg gtccgtacgg gtaccccgtc gcctccgccg gatcgatctt
     3001 cgtgttccgc gacgggatgc tgcgccgcat cgacccggcg tcgctgtcgg agacggggtc
     3061 gtggacggtc ccgggtctga gcaccgacct ctgggtgacg agcgtcggcg gcgacatcgt
     3121 ctgggtgact cgctcgtccg aggccccctt cgacgagcag ctctaccggt tcgatccggt
     3181 caccggcacg accaccacct cggggcccac gaacctcggg tacgc
//
LOCUS       DATMFS010000618         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_347485, whole genome shotgun sequence.
ACCESSION   DATMFS010000618 DATMFS010000000
VERSION     DATMFS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347485"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNQ33_11865"
     CDS             <1..355
                     /locus_tag="VNQ33_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98850.1"
                     /translation="VTFVSAVTGADAVQLVNLVVAAALFVGGCAGFGIGFLRAVGRSR
                     AETIDLAGLFYLTGSAPADVRRAFLGLWFAQIGIAVVAIPLTRPPFAVMAPVWGIGLI
                     TWWAARHATFPPRTE"
     gene            437..880
                     /locus_tag="VNQ33_11870"
     CDS             437..880
                     /locus_tag="VNQ33_11870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ98851.1"
                     /translation="MTDRATESTTIAAAPDAVLSILLDFPRYPEWAKDLKSVEVLGTD
                     DQGRATEVRFRAAGFGRSTNYTLTYDHSTPNVLAWVLSEGDIMRKLDGRYVLTEADGG
                     TEVVYELEVELVIPLPGFIKNRSQGKIMHTALGELKAFAERTAGS"
     gene            877..2115
                     /locus_tag="VNQ33_11875"
     CDS             877..2115
                     /locus_tag="VNQ33_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWJ98852.1"
                     /translation="MRVLLFTGKGGVGKTTTAAATALRCADAGQRTIVLSTDPAHSLS
                     DAYGVELGDHPVEISPGLWGQQLDAQARMEDTWQEIQAWLLEVFQWAGVAAIEAEELS
                     VIPGLDEIFALSDIKAYAESGEWDIVVVDCAPTAETLRLLSLPEILRWYMDRVFPMSR
                     KVNKVVSPLLGRVAGLPVPGDEVFGSASQFYDRLDGVRELLTDTARTSVRLVVNPERL
                     VVAEARRTYTYLSLFGYRVDAVVANRLLPDAITDPWFQEWKARHAEHLASIEEGFAPL
                     PILRAELASDELVGVGALRAFAAHLYADLDPAAVLHPGEPLQVVPKGTGYELTVELPF
                     ADKDELELGRRDDELLIRVGSHRRALLLPDSLRRREVRAASLRDGRLRVTFAGPADEG
                     IDDDPVVDRAAGASGRRRAR"
BASE COUNT          276 a          824 c          742 g          319 t
ORIGIN      
        1 ggtcaccttc gtctcggcgg tcaccggtgc cgacgcggtc cagctcgtca acctggtggt
       61 ggccgcggcc ctcttcgtgg gcggctgcgc ggggttcggg atcgggttcc tgcgggccgt
      121 cggccggagc cgtgccgaga ccatcgacct ggccgggctc ttctacctca ccggttcggc
      181 gccggccgac gtccgccggg ccttcctcgg cctctggttc gcccagatcg gcatcgccgt
      241 ggtggcgatc ccgctgaccc gcccgccgtt cgcggtcatg gcgcccgtgt ggggcatcgg
      301 cctcatcacc tggtgggccg cccgccacgc caccttcccg ccccgcaccg agtagcgcgg
      361 cactagcctg cgccctcgtg ctcggccctc gggccggtgc cgtcgagcgt ccacgacccc
      421 ggagcgtcct gcccccatga ccgaccgagc gaccgagtcg accaccatcg ccgccgcgcc
      481 cgatgccgtc ttgtccatcc tgttggactt cccccgctac cccgagtggg ccaaggacct
      541 gaagtccgtc gaggtgctcg gcaccgacga ccagggccgg gccaccgagg tgcggttccg
      601 ggcggccggc ttcggccggt cgacgaacta cacgctgacc tacgaccact cgacgcccaa
      661 cgtcctcgcc tgggtgctgt cggagggcga catcatgcgc aagctcgacg gccggtacgt
      721 gctcaccgag gccgacggcg gcaccgaggt cgtctacgag ctcgaggtgg agctggtcat
      781 cccgctgccc ggtttcatca agaaccggtc gcaggggaag atcatgcaca ccgcgctggg
      841 tgagctgaag gcgttcgcgg agcgcacggc cgggtcgtga gggtcctcct cttcaccggc
      901 aagggcggcg tcggcaagac caccaccgcc gcggccaccg cgctgcgctg cgccgatgcc
      961 ggccagcgga ccatcgtcct cagcaccgat ccggcgcact cgctgagcga tgcctacggc
     1021 gtcgagctcg gcgaccaccc cgtcgagatc tcgcccgggc tctgggggca gcagctcgac
     1081 gcccaggccc ggatggagga cacctggcag gagatccagg cctggctcct cgaggtgttc
     1141 cagtgggccg gggtggcggc gatcgaagcc gaggagctgt cggtcatccc cggcctcgac
     1201 gagatcttcg ccctcagcga catcaaggcc tacgccgaga gcggcgagtg ggacatcgtc
     1261 gtcgtggact gcgcacccac ggccgagacc ctccgcctgc tgagcctccc cgagatcttg
     1321 cgctggtaca tggaccgggt cttccccatg agccgcaagg tcaacaaggt ggtgtcgccg
     1381 ctcctcggcc gcgtggcggg gctgccggtg cccggcgacg aggtgttcgg ctcggcctcg
     1441 cagttctacg accgcctcga cggggtgcgc gagctgctca ccgacaccgc ccgcaccagc
     1501 gtccgcctgg tggtgaaccc cgagcgcctg gtggtggcgg aggcccgccg cacctacacc
     1561 tacctgtcgc tgttcggcta ccgggtcgac gccgtcgtgg ccaaccgcct cctgcccgac
     1621 gccatcaccg acccctggtt ccaggagtgg aaggcccgcc acgccgagca cctggcttcc
     1681 atcgaggagg gcttcgcccc gctccccatc ctgcgagccg agctggcgtc cgacgagctc
     1741 gtcggcgtcg gtgccctgcg tgcgttcgcc gcccacctct acgccgacct cgatccggcc
     1801 gccgtcttgc accccgggga gccgctccag gtcgtcccga agggcacggg ctacgagctc
     1861 accgtggagc tgcccttcgc cgacaaggac gagctggagc tgggccgccg cgacgacgag
     1921 ctgctcatcc gggtcgggag ccaccggcgg gcgctgctgc tgcccgactc cctgcgccgc
     1981 cgggaggtcc gggccgcgtc gctgcgcgac ggccggctcc gggtgacctt cgccggtccg
     2041 gccgacgagg gcatcgacga cgacccggtc gtcgaccggg ccgcaggtgc ctcgggccgg
     2101 cggcgggccc gatgaccgcc tccgacgagg gcaccgtgcc gcccggcgcc tgggatgatc
     2161 a
//
LOCUS       DATMFS010000619         2819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21651, whole genome shotgun sequence.
ACCESSION   DATMFS010000619 DATMFS010000000
VERSION     DATMFS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21651"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1524)
                     /locus_tag="VNQ33_11880"
     CDS             complement(100..1524)
                     /locus_tag="VNQ33_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWJ98853.1"
                     /translation="MHTRTWRLLGAAAAFVALTTAAVGCGSDGNSSASSSTTKAESGG
                     DGGSTITVPGDYKTIQEAVDAAKKGDLVLISEGTYKEAVDVTTPDITIRGVDRNKVIL
                     DGGFELENGIRVLDTDGVTVQNMTAKNYVSNGFFWTGSDRYHGSYLTAYRNGDYGIYA
                     FDAYHGQLDHSLGSGSPDAGFYIGECYECDAVIDTVVSEHNGLGYSGTNSGGELYIIN
                     STFRYNRAGVVPNSGSYELCYPGRSNTIVGNTVYDNNNDDAPAIDVALLAQGNGILIA
                     GSINNTIERNLVYDHDRTGIAAVPFPEEDASDVVPPASELKTPCEETKDDELPAEGDV
                     PDIVMWNATGNSIVGNDVSGSGLADLASGTIALDDKSATTADLDNCFSDNVFTSSAPA
                     DLEGLAPCEGEGNGGDFEKDGLDLLVLINEQAPAPPKDTYKSTPEPGTQENMPNAKTA
                     PAPIFQGPEKPDVDAIKVPTKPSN"
     gene            1853..>2819
                     /locus_tag="VNQ33_11885"
     CDS             1853..>2819
                     /locus_tag="VNQ33_11885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWJ98854.1"
                     /translation="MISHRRPRRNAVRGTAAALAALLVVAAVAVSIGTDPAAVAVLVI
                     VGAAVGAIVSAVDRASALVSLRHQVLEVLDSFTAALGTAVVDGDLHRTLLEQMGDRLH
                     AETAWIQSADRPDERIVWHADGQPLRADELADADDGRERGDAIIGTVRVGDARLLVAV
                     EHRAGSVRAFDGEDRRLFDLLVAHAGIALQNVRLVDQLRHEARLNEELATRDQLTGLP
                     NRTKFLRDLDRLPPTGPVGVLLLGVDRFQEVNDTLGHQRGDELLIEVGKRLLAAVDGE
                     ASIARLAGDEFAVRWPTADAHELLRRSRSVLSAVNGLYELAGMELD"
BASE COUNT          373 a          957 c         1016 g          473 t
ORIGIN      
        1 aggtgccgcc cgagccaccg cgggcgagcg ccgccgagac cgccgtcacc acgacgacga
       61 ccccggccgc cgcgaccagc gcccggcggc ggcccgtgct caattgctgg gcttggtcgg
      121 caccttgatg gcgtcgacgt cgggcttctc cgggccctgg aagatgggag cgggcgccgt
      181 cttggcgttc ggcatgttct cctgcgtccc gggctccggg gtggacttgt aggtgtcctt
      241 cggcggggcg ggagcctgct cgttgatcag cacgaggagg tcgaggccgt ccttctcgaa
      301 gtcgccgccg ttcccctcgc cctcgcaggg agccagcccc tcgaggtcgg ccggggcgct
      361 gctggtgaac acgttgtcgg agaagcagtt gtcgaggtcg gccgtggtgg cggacttgtc
      421 gtcgagggcg atggtgcccg aggcgaggtc agcgaggccc gagcccgaga cgtcgttgcc
      481 cacgatggag ttgcccgtcg cgttccacat cacgatgtcg gggacgtccc cctcggcggg
      541 aagctcgtcg tccttggtct cctcgcaggg ggtcttcagc tcgctggccg gcggcacgac
      601 gtcgctcgcg tcctcttcgg ggaacggcac ggcggcgatg ccggtccggt cgtggtcgta
      661 gacgaggttg cgctcgatcg tgttgttgat gctgcccgcg atcaagatgc cgttgccctg
      721 ggccaggagg gcgacgtcga tggctggggc gtcgtcgttg ttgttgtcgt agacggtgtt
      781 gccgacgatg gtgttggagc ggcccgggta gcagagctcg taggaaccgg agttcggcac
      841 gacaccggcc cggttgtacc ggaacgtcga gttgatgatg tagagctcac cgcccgagtt
      901 ggtgccggag tagccgaggc cgttgtgctc ggacacgacg gtgtcgatca ccgcgtcgca
      961 ctcgtagcac tcaccgatgt agaagccggc gtccgggctg cccgacccga ggctgtggtc
     1021 gagctggccg tggtacgcgt cgaaggcgta gatgccgtag tcgccgttgc ggtaggccgt
     1081 gaggtacgag ccgtggtagc ggtccgatcc cgtccagaag aacccgttcg acacgtagtt
     1141 cttggccgtc atgttctgga ccgtcacccc gtccgtgtcg aggacgcgga tgccgttctc
     1201 gagctcgaag ccaccgtcga ggatcacctt gttgcggtcg acgccgcgga tggtgatgtc
     1261 cggggtggtg acgtcgacgg cctccttgta ggtgccctcg ctgatgagga cgaggtcgcc
     1321 cttcttcgcc gcgtccaccg cctcctggat cgtcttgtag tcgccgggaa cggtgatcgt
     1381 cgatcccccg tcgccgccgg actcggcctt ggtcgtggag gagctggcgc tcgagttccc
     1441 gtcgcttccg cagccgaccg cggcggtggt gagggccacg aaggccgcag cggcgccgag
     1501 caggcgccag gtccgggtgt gcatgtcatt tcccccttgc aggcgaacca ctggtgtcgc
     1561 caaagaacac agtcggcagc atcctgccac agcgagagcg ccaccccgct cgtgagcggc
     1621 agcggcgggc tgcgccagga ccagggtgga tggccgggcg ccggacggtc acccggccga
     1681 agcgcggtcc gttccccctc gatcggccac gcatcgtgag ggaattgtcc gggacccggc
     1741 ttgacatgct gcggagcgtg atatcaaagt ggtcgatctc acctcacgtg gttgaatgac
     1801 cgcgagctgt ggggagggct cgtcggaggg gcgagctggg gaggaagacc tcgtgatcag
     1861 ccatcgacgt ccgcggcgga acgcggtccg tgggaccgct gccgcgctcg ccgccctgct
     1921 cgtcgtcgcc gccgtcgcgg tctccatcgg gaccgacccc gccgccgtcg ccgtgctcgt
     1981 catcgtcggc gccgccgtcg gcgccatcgt cagcgccgtg gatcgggcct ctgccctggt
     2041 ctcgctccgc caccaggtcc tcgaggtgct cgactcgttc accgccgcgc tcggcacggc
     2101 cgtcgtcgac ggcgatctcc accggacgct cctggagcag atgggagacc ggctgcacgc
     2161 ggagaccgcc tggatccagt ccgccgatcg gcccgacgag cggatcgtct ggcacgccga
     2221 cggccagccg ctgcgcgccg acgagctcgc cgacgcggat gacggccgtg agcgcggcga
     2281 cgcgatcatc ggcacggtgc gcgtgggcga cgcccgcctc ctcgtcgccg tcgagcaccg
     2341 cgccggcagc gtgcgggcgt ttgacggcga agaccggcgg ttgttcgatc tgctcgtcgc
     2401 ccacgccggc atcgccctcc agaacgtgcg gctcgtcgac cagctccgcc acgaggcgcg
     2461 gctcaacgag gagctggcga cccgtgacca gctcaccggg ctccccaacc gcaccaagtt
     2521 cctccgggac ctggatcgcc tgccgccgac tggcccggtc ggggtgctgc tcctcggggt
     2581 cgaccggttc caggaggtca acgacaccct cggccaccaa cgtggcgacg agctgctgat
     2641 cgaggtgggc aagcgcctcc tcgcggcggt cgatggcgag gcctcgatcg cccggctcgc
     2701 cggtgacgag ttcgctgtgc ggtggcccac cgccgacgcc cacgagctgc tgcgccggtc
     2761 ccgcagcgtg ctcagcgcgg tcaacggcct ctacgagctc gccggcatgg agctcgacg
//
LOCUS       DATMFS010000620         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_347499, whole genome shotgun sequence.
ACCESSION   DATMFS010000620 DATMFS010000000
VERSION     DATMFS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347499"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VNQ33_11890"
     CDS             complement(<1..1168)
                     /locus_tag="VNQ33_11890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ98855.1"
                     /translation="MKVTFVALNYAPSRGGAQEHVRRVAEGLVARGHDVTVLTTDALR
                     SPASREPGRIRDRRARIGGVEVRRYPTPLLLQWAMAVLRRLVWRQRQRAGEPGAVPEA
                     VPLGPWLLGPWSPSLALAVRRAMRSDDVVIGCSAPFTTAVLPNWLRVRGGARVASMPL
                     LHASTTGPHPALGRALRTSDLVFASTDFEARIDRSVGISSLRVGVVPPGVAPLDYPVL
                     DPQDARARCALPERPTVGFIGRLAAYKGVDTLLDAAPAIWEAAPDTTVLIAGSATGWD
                     GFRSPAVANVGGDRLVIREGFRDEERAWLYAACDVVVHPSRDESFGLVALESWAARRP
                     VVLADIPCVRSFAEEGRTAEIVPPGDPASLAQKVVDLLGDPARRERLAAAGNAEI"
     gene            complement(1165..1908)
                     /locus_tag="VNQ33_11895"
     CDS             complement(1165..1908)
                     /locus_tag="VNQ33_11895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWJ98856.1"
                     /translation="MSLGPASASRWLRFRTSVVDRLVALVLAPVLAPAVAVLGWRVKQ
                     GDGGPPLIALPRVGQRCRPFAMWKLRSMRAERRDGAAEGAVVTAADDDRITPIGATMR
                     RWRLDELPQVWNVIRGDMGLLGPRPETPSMVDAADPRWIAVCAVRPGVSGPTQLVVER
                     WEAAVMHAESAADRYRGEILPVKLAIDRWYVEHATPVTDVQVVWSMLERFALGREETW
                     IERVVRARVPEAEAVPLDGARSHEGAGAP"
BASE COUNT          299 a          769 c          769 g          217 t
ORIGIN      
        1 cgatctcggc gttgccggcc gcagcgaggc gctcccggcg ggccgggtca ccgaggagat
       61 cgaccacctt ctgcgccagg ctcgcaggat cgcccggagg cacgatctcg gcggtgcggc
      121 cctcctcggc gaagctccgc acgcacggga tgtcggccag caccacggga cgccgggccg
      181 cccaggactc gagggcgacg agcccgaacg actcgtcgcg cgacggatgg accaccacgt
      241 cgcaggcggc gtacagccac gcccgctcct cgtcgcggaa gccctcgcgg atcaccaggc
      301 gatccccgcc gacgttggcc acggcgggcg agcggaaccc gtcccaaccg gtcgcgctcc
      361 cggcgatgag caccgtggtg tcgggggccg cctcccagat cgccggtgcc gcgtccagga
      421 gcgtgtcgac gcccttgtac gccgccagcc tgccgatgaa ccccacggtc ggacgctcgg
      481 ggagggcgca ccgagcacgg gcgtcctgcg ggtcgaggac cgggtagtcg agcggggcca
      541 cccccggggg cacgacgccg acgcgcaacg aggagatccc gacggagcgg tcgatgcggg
      601 cctcgaagtc cgtggaggcg aacacgaggt cgctcgtgcg cagagcccgg cccagcgctg
      661 ggtgcgggcc ggtggtcgac gcgtgcagga gcggcatcga ggcgacccgg gcgccgccgc
      721 ggacgcggag ccagttcggc aagacggccg tcgtgaaggg cgccgagcag ccgatgacca
      781 cgtcgtcgga gcgcatggcc cgccggaccg ccagggcgag gctcggcgac cagggaccga
      841 ggagccaggg ccccagaggc accgcctcgg ggacggcgcc cggctccccg gcgcgctgac
      901 gctgccgcca gacgagccgg cgcagcaccg ccatcgccca ctggaggagg agcggggtcg
      961 ggtagcgccg cacctccacc ccgccgatcc gggcgcgccg gtcgcggatc cggcccggct
     1021 cgcgagaagc cggtgagcgg agggcgtcgg tggtgagcac ggtgacgtcg tggccgcggg
     1081 ccaccagacc ctcggcgacg cgccggacgt gctcttgggc gccaccgcgc gacggcgcgt
     1141 agttcaaggc gacgaacgtg accttcacgg cgcacccgcg ccttcgtgcg accgcgcgcc
     1201 gtcgagcggg accgcctctg cttcggggac ccgcgcgcgg acgacccgct cgatccaggt
     1261 ctcctcgcgt cccagcgcga accgctcgag catcgaccag acgacctgga cgtcggtcac
     1321 gggggtggcg tgctcgacgt accagcggtc gatcgccagc ttcaccggga ggatctcacc
     1381 gcggtagcgg tcagccgcgg actcggcgtg catcaccgcc gcctcccacc gctccacgac
     1441 gagctgcgtc ggtccggaga cgccgggccg cacggcgcac acggcgatcc agcgggggtc
     1501 tgccgcgtcg accatcgaag gcgtctccgg ccgggggccg agcaggccca tgtccccgcg
     1561 gatcacgttc cacacctgcg gcagctcatc gagtcgccac cggcgcatcg tggcgccgat
     1621 cggcgtgatg cggtcgtcgt cggcggcggt gacgaccgct ccttccgccg ctccgtcgcg
     1681 gcgctccgca cgcatcgaac ggagcttcca catcgcgaac gggcggcacc gctggccgac
     1741 gcggggaagg gcgatgagcg gcgggccgcc gtcgccctgc ttgaccctcc atccgagcac
     1801 ggcgacggcg ggggccagga ccggtgcgag caccagcgcc accaagcggt cgacgaccga
     1861 ggtccgaaag cgcagccagc gcgacgcgct cgcaggcccg aggctcacga cagcgcgcca
     1921 ccgagcgtgt cgacgacccg atctacctcc tgatcggtga gcccggcatg cagcggcagg
     1981 ctcagggctc gtccggcgag acgatcggcg gcggcgaccc cggtggggcc gaccggggcg
     2041 tggtcgcgga acca
//
LOCUS       DATMFS010000621         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_252045, whole genome shotgun sequence.
ACCESSION   DATMFS010000621 DATMFS010000000
VERSION     DATMFS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252045"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VNQ33_11900"
     CDS             complement(<1..615)
                     /locus_tag="VNQ33_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98857.1"
                     /translation="MAGNASITSVDLDGERAALARARDAVQAKLTSLAGISGGGADAL
                     ADEYIDAVVRGAVEKLQQELVVFGRIDDDQPWRVGLYGIDEDGDQLVIDWRAPFAGGF
                     YQARLDETMGLDRRVTYVGSITDLFVEEFTTGEISGSSPLLGELSRSRGTEMRAAVAT
                     LQSEQDALVRLDPTARLVLRGGPGTGKTVVGLHRAAWLVYNDRRL"
     gene            complement(789..2111)
                     /locus_tag="VNQ33_11905"
     CDS             complement(789..2111)
                     /locus_tag="VNQ33_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98858.1"
                     /translation="MQYTRLVIEQGTTTFSLGFHARLTVVAGVEAGARKGLVAELIGG
                     LGSTRTGVNLELADDRGRRLAVLRPATGGHRVIDLTSGTDVSEEFRSPDGRLDILGRY
                     GIDTRTAADALHLDRNGLARDAQRDHIVQRLSEIDQTELWSTAARVRITEDELKALGE
                     ERSGQDDAELVARIERHHHGLEEALEQHRKLRRDASLVCGVSLLAALPVGLIRPGLAI
                     PILAIGLVTILMAFMFRARVEAVQRMEKSALADAGVDSYLGYVVGRVNDMMEDTESRR
                     RRLALSEDHRAAAVRWTRVAGDISVEWALAHHEEIETAARLRRELATLGQISTTAPAL
                     DDETTAIAQSLISHLGRLRTIGSGAESMPLVLDDPFTDVAPSTKLTLMELLARTAGSP
                     QVILLTDQDEVATWARLEALTGEVALVEPSISTQPTTRPGNRSNDLAV"
     gene            2285..2357
                     /locus_tag="VNQ33_11910"
     tRNA            2285..2357
                     /locus_tag="VNQ33_11910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2318..2320,aa:Thr,seq:ggt)
     gene            2374..2450
                     /locus_tag="VNQ33_11915"
     tRNA            2374..2450
                     /locus_tag="VNQ33_11915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2408..2410,aa:Met,seq:cat)
     gene            2520..>2655
                     /locus_tag="VNQ33_11920"
     CDS             2520..>2655
                     /locus_tag="VNQ33_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ98859.1"
                     /translation="MGKEKFERNKPHLNIGTMGHIDHGKTTLTAAITKTLHDADPTTA
                     F"
BASE COUNT          395 a          853 c         1001 g          406 t
ORIGIN      
        1 gaggcggcgg tcgttgtaga cgagccacgc ggcgcggtgg agcccgacga ccgtcttgcc
       61 ggtcccgggg ccgccgcgca ggacgaggcg ggcggtgggg tcgaggcgga ccagggcgtc
      121 ctgctccgat tggagcgtgg cgacggcggc gcgcatctcg gtgccgcggc tgcgggacag
      181 ctcgccgagc agcggtgacg agccgctgat ctcgccggtg gtgaactcct cgacgaacag
      241 gtcggtgatg gagcccacgt aggtgacccg gcggtcgagc cccatcgtct cgtcgaggcg
      301 agcctggtag aagccgccgg cgaacggggc ccgccagtcg atgaccaact ggtccccgtc
      361 ctcgtcgatg ccgtagagcc cgacccgcca cggctggtcg tcgtcgatgc ggccgaacac
      421 caccagctcc tgctggagct tctccactgc gccccgcacg actgcatcga tgtactcgtc
      481 ggccagagcg tcggcaccgc cgcccgagat gccggccaag gaggtgagct tggcctggac
      541 cgcatcacgg gcgcgcgcca gggcggctcg ttcgccgtcg aggtcgacgg aggtgatcga
      601 tgcgttgcca gccatgcgcg cccgtcccaa taggttccaa ccgggcggac gacgctagtg
      661 ccgcgccggg tgcagcggtc agcccatttc cgaacttcgt gtgggaccgg tccacgaacg
      721 aacccgatcc acacggcgtt cgcgccgggg tggtggcggg aggtgccggg tggggagcag
      781 ggcgggggtc agacggcgag gtcgttggag cggttgccgg ggcgggtggt cggctgcgtc
      841 gagatcgacg gctccacgag ggcgacctcg ccggtcaggg cttcgaggcg agcccacgtg
      901 gcgacctcgt cttgatcggt gaggaggatg acctggggcg atccggcggt gcgggcgagc
      961 agctccatca gcgtcaactt ggtggacggc gccacgtcgg tgaacgggtc gtcgaggacg
     1021 agcggcatgc tctcagcgcc cgagccgatg gtccggagcc ggccgaggtg gctgatgagc
     1081 gactgggcga tggccgtggt ctcgtcgtcg agcgccggtg ccgtcgtcga gatctggccg
     1141 agcgtggcca gctcgcggcg caggcgggcc gccgtctcga tctcctcgtg gtgggcgagc
     1201 gcccactcga cgctgatgtc accggcgacg cgggtccagc gcacggccgc ggcccggtgg
     1261 tcctcggaga gggcgaggcg ccggcggcgg gactcggtgt cttccatcat gtcgttgacc
     1321 cggccgacga cgtacccgag gtacgagtcc accccggcgt cggcgagggc gctcttctcc
     1381 atgcgctgca cggcctcgac gcgggctcgg aacatgaagg ccatgaggat ggtgacgagg
     1441 ccgatggcga ggatcgggat cgcgaggccc ggacggatga gcccgacggg gagggccgcc
     1501 agcagcgaga cgccgcagac cagcgaggcg tctcggcgga gcttccggtg ctgttcgagc
     1561 gcttcttcga gaccgtggtg gtgccgctcg atgcgggcga cgagctcggc gtcgtcctgc
     1621 ccggagcgct cctcgccgag ggccttcagc tcgtcttcgg tgatgcggac gcgcgcggcc
     1681 gtggaccaca gctcggtctg gtcgatctcg ctcaggcgct ggacgatgtg gtcgcgctga
     1741 gcatcgcggg cgagtccgtt gcggtcgagg tggagggcat cggcggccgt gcgggtgtcg
     1801 atgccgtacc ggcccaggat gtcgagccgc ccgtcggggc tgcggaactc ctcgctcacg
     1861 tcggtcccgc tggtcaggtc gatgacgcgg tggccaccgg tcgcgggacg cagcaccgcg
     1921 agccgccggc cccgatcgtc ggccagttcg aggttgacac cggtgcgcgt gctgccgagc
     1981 cccccgatca gctcggcgac caggcccttt cgtgcacccg cctcgactcc tgccaccacc
     2041 gtcagccgtg catggaagcc gagcgagaac gtggtggtgc cctgctcgat gaccagacgg
     2101 gtgtactgca ccgcggacca tcggccggag ggggctgttg ttgagcgatt cgtgggccaa
     2161 acggcccaga ttcgtcgccc ccgacggtga tggcgctgtc aggcggatct ggctttcgtg
     2221 ccgggtgagg gaggtggcta tgctgaccag ccggtccgaa ccgctccaga gcggcgcggc
     2281 ccttgcctcc ttagctcagt cggcagagcg cttccatggt aaggaagagg tcgtgggttc
     2341 gattcccaca ggaggctcgc aaagccagat cctggcggcg tagctcagtt ggtcagagca
     2401 ctcggctcat aatcgagttg tcaccggttc gaacccggtc gccgctacca agcgcctggg
     2461 ccgtccgccg gtccgagcga gtccgtccaa gcaagaacac caggcaaggg agtccggtca
     2521 tgggcaagga aaagttcgag cggaacaagc cgcacctgaa cattggcacg atgggtcaca
     2581 tcgaccatgg caagacgacg ttgacggcgg cgatcacgaa gacgctgcat gatgcggatc
     2641 cgacgacggc gttca
//
LOCUS       DATMFS010000622         5286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_229605, whole genome shotgun sequence.
ACCESSION   DATMFS010000622 DATMFS010000000
VERSION     DATMFS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229605"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="treS"
                     /locus_tag="VNQ33_11925"
     CDS             complement(<1..1269)
                     /gene="treS"
                     /locus_tag="VNQ33_11925"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWJ98860.1"
                     /translation="MTTDDPSWYQRAIFYEVLVRGFRDSNGDGTGDLRGITEKLDYLE
                     WLGVDCLWLLPFYSSPLKDGGYDISDFFTVLPDYGTVGDAAELIDEAHRRGIRVIADM
                     VMNHTSDQHPWFQESRSSRDNPKHDWYVWNDDNDRWSEARVIFVDTEPSNWSWEPQRE
                     QYYWHRFFHHQPDLNYDNPEVAEVMLDVVRHWLGLGLDGFRLDAVPYLFERDGTNGEN
                     LKETHAYLQKVRSVVDQEFPGKILLAEANQWPADVVDYFGEPDAPECHLCFNFPLMPR
                     MFMAARQETSRPIREIMAQTPEIPGGGQWGIFLRNHDELTLEMVTDEERDYMYAEYAK
                     DPRMKRNVGISRRLLPLLENDRRMAELFHGLLFSLPGSPVMYYGDEIGMGDNIFLGDR
                     DGVRTPMQWTPDRNGGFSTADFAQLYLPPLM"
     gene            1525..2442
                     /locus_tag="VNQ33_11930"
     CDS             1525..2442
                     /locus_tag="VNQ33_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98861.1"
                     /translation="MLLRHRTLVRTAMGVAALSAVLLTACEPTTVPTKPGTQRVLVLG
                     DSIPSWLIKDGTKTINQATTTLVDGTLEACDGARSNPPARAVTGKVVPTPAACAKGWP
                     SMYPPYLKIRPDVAIVMTSTHAMLDHQLDGTWRHPCHAPAHAWYQNDMAARLAYLKSH
                     AGRVVVVLPAWPGTNSGWIMPKDSVKRADCVRADIRAAARTAGVTAIDFGAYLCPDGP
                     SACKPWRTKDGMHIDPVRAPSALGWVTGTAMQTGADAATSAVTAQPVTTTSSTTTSTT
                     TTTTSMPVPTAPSTTTSSTSTTTSAPPPT"
     gene            complement(2443..3027)
                     /locus_tag="VNQ33_11935"
     CDS             complement(2443..3027)
                     /locus_tag="VNQ33_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HWJ98862.1"
                     /translation="MTDPQILPFDVPAAVDEVIRSRRTSLQVDPDDPVPDELIARLVE
                     LATWAPNHKRTWPWRFTVITGAARHRLGEALAMVAPDLGVPPEKAAKLRTKYARSPAV
                     VLVWVRVDPSPLRAKEDRDAVAAAVQNLLLAATSFGLGNYWATVPEVLEDGVRRFAHV
                     GDDHDLVALVYLGWPTATAPAPPRPEPEVTWFDG"
     gene            3292..>5286
                     /gene="glgP"
                     /locus_tag="VNQ33_11940"
     CDS             3292..>5286
                     /gene="glgP"
                     /locus_tag="VNQ33_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWJ98863.1"
                     /translation="MRALRSFTVRPRLPEPLAPLEELAMNLRWAWDDRTRDLFRWVDP
                     DAWDATRHDPVSMLGTTSPARLAELAADPAFLRFQTSVHEDLTRYLEAPRWFQNRESG
                     LRQVAYFSPEFGIAEALPQYSGGLGILAGDHLKSASDLGVPLVGVGLFYRHGYFRQGL
                     DQEGWQQERFPDQDPFAMPLTRCEGIRVTVDLAGTPMQAQVWRADVGRIPLYLLDADV
                     EENPDEIRAVTDRLYGGDVEHRLRQEILLGIGGVRALDALGIDAQVFHTNEGHAGFLG
                     LERMRRSIVDDGLTFDEAVEATRSASIFTTHTPVPAGIDRFPRALIETYFGAWAAECR
                     ISIDQLMALGNRPSETASDPADQRFNMAVMGLRLAGRSNAVSALHGEVSRAMFQELWP
                     DLDDEEVPIGSVTNGVHSGTWVSADMTDLLARHVLPDWDEAGTEAWTRVWSTPDDELW
                     RVKEQARARLVAFVRERVKLRAVAGGISASEATWADDLLDPNALTIGFARRFATYKRA
                     NLLLAQAERLQALLLSTDRPVQFVFAGKAHPADDEGKQMISEIVSFAKELGVRHRFVF
                     LDDYDISVARALYQGADIWLNTPRRPQEACGTSGMKAALNGTLNCSILDGWWDELFDG
                     ENGWAITSAEDLDDLAARDRVEADSLFDLLEHQIVPLFHDR"
BASE COUNT          781 a         1978 c         1763 g          764 t
ORIGIN      
        1 catcagcggc ggcaggtaga gctgggcgaa gtccgccgtg gagaacccgc cgttgcgatc
       61 cggggtccac tgcatcgggg tgcggacgcc gtcgcggtcg ccgaggaaga tgttgtcgcc
      121 catcccgatc tcgtcgccgt agtacatgac cgggctgccg ggcaggctga agagcaggcc
      181 gtggaacagc tccgccatgc ggcggtcgtt ctccagcagc gggaggaggc gccgggagat
      241 gcccacgttg cgcttcatgc gcgggtcctt ggcgtactcc gcgtacatgt agtcccgctc
      301 ctcgtcggtg accatctcca gggtcagctc gtcgtggttg cgcaagaaga tgccccactg
      361 cccgccgccg gggatctccg gggtctgggc catgatctcg cggatgggcc gggaggtctc
      421 ctggcgggcg gccatgaaca tgcgcggcat cagcgggaag ttgaagcaca ggtggcactc
      481 gggtgcgtcg ggctcgccga agtagtcgac gacgtcggca ggccattggt tcgcctcggc
      541 caggaggatc tttccgggga actcctggtc gaccacgctg cgcaccttct gcaggtaggc
      601 gtgggtctcc ttcaggttct cgccgttcgt cccgtcgcgc tcgaacaggt agggcaccgc
      661 gtcgagccgg aagccgtcga ggccgagccc cagccagtgg cgcacgacgt cgagcatgac
      721 ctcggccacc tcggggttgt cgtagttcag gtcgggctgg tggtggaaga accggtgcca
      781 gtagtactgc tcgcgctggg gctcccagct ccagttcgac ggctcggtgt cgacgaagat
      841 cacccgggcc tcggaccacc ggtcgttgtc gtcgttccac acgtaccagt cgtgcttcgg
      901 gttgtcgcgc gacgagcggg actcctggaa ccacgggtgc tggtcgctcg tgtggttcat
      961 caccatgtcg gcgatgaccc ggatcccccg gcggtgcgcc tcgtcgatga gctcggcggc
     1021 gtcgcccacg gtcccgtagt cggggagcac ggtgaagaag tccgagatgt cgtagccgcc
     1081 gtctttgagc ggcgagctgt agaagggcag cagccagagg cagtcgacgc cgagccactc
     1141 caggtagtcg agcttctcgg tgatgccccg gaggtcgccg gtgccgtcgc cgttggagtc
     1201 gcggaacccg cggaccagga cctcgtagaa gatggcccgc tggtaccagc tcgggtcgtc
     1261 ggtggtcatc gggcgatcgt acccacgcgc cgcgtcgcgc cagacgggtg gctcagcgcg
     1321 gcgcacggcg gggaccgtcg ggggagccgg tgcggacacc atgctccccc tcgggtcccg
     1381 ccccaggacg ccgcctcgtc cgaggaactc gggacaggtt tccactgttc accatcccgc
     1441 gccacggtct gtgtccaacg tcacatcgca cggacggacc gtggccaaga caccacacct
     1501 gccgggctgg cacactcggc tccgatgctg ctgcgacacc ggacgctggt ccgcacggcg
     1561 atgggggtgg cggccctgtc cgccgtgctc ctcaccgcct gcgaacccac caccgtgccc
     1621 accaagccgg gcacccagcg ggtcctcgtc ctgggcgact ccatcccgag ctggctcatc
     1681 aaggacggga cgaagaccat caaccaggcc acgacgacat tggtcgacgg caccctcgag
     1741 gcgtgcgacg gcgcccgcag caacccgccg gcccgcgccg tcaccggcaa ggtcgtcccc
     1801 actcctgcag cctgcgccaa gggctggccg tcgatgtacc cgccctacct gaagatccgc
     1861 cccgacgtcg ccatcgtcat gacgagcacc cacgccatgc tcgaccacca gctcgatggc
     1921 acctggcgcc acccctgcca cgcccccgcc cacgcctggt accagaacga catggcggct
     1981 cgcctcgcgt acctcaagag ccacgccggt cgggtcgtcg tcgtcctgcc cgcctggccc
     2041 gggaccaaca gcgggtggat catgccgaag gactccgtga agcgcgccga ctgcgtccgg
     2101 gccgacatcc gggcggcggc caggaccgcc ggcgtcacgg ccatcgactt cggcgcctac
     2161 ctctgccccg acggtccgag cgcctgcaag ccctggcgca ccaaggacgg catgcacatc
     2221 gaccccgtcc gtgccccctc cgccctgggc tgggtgaccg ggacggcgat gcagacgggc
     2281 gccgacgcgg ccacgtccgc cgtcacggcg cagccggtca ccacgacctc gagcaccacg
     2341 acctcgacga cgacgaccac cacctcgatg ccggtgccga cggcgccatc caccaccacg
     2401 tcgtccacca gcaccacgac ctcggcgccg ccgccgacgt gatcagccgt cgaaccaggt
     2461 gacctccggt tcgggacgcg gcggcgccgg cgccgtggcg gtgggccagc cgaggtacac
     2521 gagcgccacc aggtcgtggt cgtcgccgac gtgggcgaag cgccggacgc cgtcctcgag
     2581 cacctccggc acggtggccc agtagttgcc gaggccgaac gacgtggccg ccaggaggag
     2641 gttctgcacg gcggccgcca cggcgtcgcg gtcctccttc gcccggagcg ggctcgggtc
     2701 gacccgcacc cacacgagca ccacggcagg gctgcgggcg tacttggtgc gcagcttggc
     2761 tgccttctcg ggcggcacac cgaggtccgg tgccaccatc gccagcgcct cgcccaggcg
     2821 gtgccgggcc gcaccggtga tcacggtgaa gcgccacggc cacgtgcgct tgtggttcgg
     2881 cgcccaggtg gccagttcca cgagccgtgc gatgagctcg tcgggcaccg gatcgtcggg
     2941 atccacctgc aggctcgtcc gccgcgagcg gatgacctcg tcgaccgcgg cgggcacatc
     3001 gaacggcagg atctgcgggt cggtcaccct gcgagtgtgc ccgaggccac gcacaacccc
     3061 gaccccgccc ccgaccccgc ccccgccccc ccgccctgat cctccaactt ggaggatcag
     3121 ggtcgcataa cgccaacatc ctccaagttg gacgaacggg gcagaagttg gaggatcgat
     3181 gtcggatggg ggcacaaacc ttccaggttg cgccggcctc aggcgatgac gggcgcgtga
     3241 acttcgccgc ctccaggtga ccttctcgga tggcctcgct accgtcgggc catgcgagcg
     3301 ctccggagct tcaccgtccg tccccgcctg ccggagccgc tggccccgct cgaggagctg
     3361 gcgatgaacc tccggtgggc ctgggacgac cgcacccgcg acctgttccg ctgggtcgat
     3421 cccgatgcgt gggacgccac ccgccacgac ccggtgtcga tgctggggac cacctccccc
     3481 gctcgcctcg ccgagctggc cgccgacccg gcgttcctgc ggttccagac ctcggtgcac
     3541 gaggacctga cccggtacct ggaagcgccg cgctggttcc agaaccgcga gagcggtctg
     3601 cgccaggtgg cgtacttctc gcccgagttc ggcatcgccg aggccctccc ccagtactcc
     3661 ggcggcctcg ggatcctcgc cggtgaccac ctcaagtcgg ccagcgacct cggcgtgccg
     3721 ctcgtcggcg tcgggctctt ctaccggcac ggctacttcc gccaggggct ggaccaggag
     3781 ggctggcagc aggagcggtt cccggaccag gaccccttcg ccatgcccct cacccgctgc
     3841 gagggcatcc gcgtcacggt cgacctggcc ggcacgccga tgcaggccca ggtgtggcgg
     3901 gccgacgtcg gccgcatccc gctgtacctg ctcgacgccg acgtcgagga gaaccccgac
     3961 gagatccgcg ctgtgaccga ccggctctac ggcggcgacg tcgagcaccg gctgcgccag
     4021 gagatcctcc tcggcatcgg cggggtccgg gcgctggacg cgctcggcat cgacgcccag
     4081 gtgttccaca ccaacgaggg ccacgccggc ttcctcggcc tcgagcgcat gcggcggtcg
     4141 atcgtcgacg acggcctcac cttcgacgag gccgtcgagg ccacccgctc ggcctcgatc
     4201 ttcaccaccc acacgcccgt gcccgccggc atcgaccggt tcccccgtgc cctcatcgag
     4261 acgtacttcg gcgcctgggc ggccgagtgc cgcatctcga tcgaccagct catggcgctc
     4321 ggcaaccggc ccagcgagac cgcttctgac cccgcggacc agcgcttcaa catggcggtc
     4381 atgggcctgc ggctcgccgg acggtccaac gccgtgtccg ccctccacgg cgaggtcagc
     4441 cgcgccatgt tccaggagct ctggcccgac ctcgacgacg aggaggtgcc catcgggtcg
     4501 gtcaccaacg gtgtgcacag cggcacctgg gtgtcggccg acatgaccga cctcctcgcc
     4561 cgccacgtcc tgcccgactg ggacgaagcc ggcaccgagg cctggacccg cgtgtggtcc
     4621 acccccgacg acgagctctg gcgcgtcaag gagcaggccc gcgcccggct ggtggcgttc
     4681 gtccgcgagc gggtgaagct ccgcgccgtc gccggcggca tctccgcctc cgaggccacg
     4741 tgggccgacg acctgctgga cccgaacgcc ctcaccatcg gcttcgcccg ccgcttcgcc
     4801 acctacaagc gggccaacct cctcctcgcc caggccgagc ggctccaagc cctgctgctg
     4861 tccacggacc ggccggtgca gttcgtgttc gccggcaagg cccacccggc cgacgacgag
     4921 ggcaagcaga tgatcagcga gatcgtctcg ttcgccaagg agctcggggt ccggcaccgg
     4981 ttcgtgttcc tggacgacta cgacatctcc gtcgcccggg cgctctacca gggcgccgac
     5041 atctggctga acacgccccg ccgcccgcag gaggcgtgcg ggaccagcgg catgaaggcg
     5101 gcgctgaacg gcaccctcaa ctgctcgatc ctcgacggct ggtgggacga gctgttcgac
     5161 ggcgagaacg gctgggccat cacctcggcc gaagacctcg atgacctcgc ggcccgcgac
     5221 cgtgtcgagg ccgacagcct cttcgacctg ctcgagcacc agatcgtgcc cctcttccac
     5281 gaccgg
//
LOCUS       DATMFS010000623         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_137900, whole genome shotgun sequence.
ACCESSION   DATMFS010000623 DATMFS010000000
VERSION     DATMFS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137900"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>2340
                     /locus_tag="VNQ33_11945"
     CDS             65..>2340
                     /locus_tag="VNQ33_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWJ98864.1"
                     /translation="MSEKLQLELSVLLPDALDRRDGCVHRLTETLDGAPGVDDVHLVD
                     GAEGGTARLCLHYDPAATSLSRIRARAEAAGAALTERYAHVLWPVDGIGHIRKARSVG
                     ESLSRVDGVMEADVAPGFVRVELDRTKLDEDALRAALADRKVSVADGRGDHEHGTGAH
                     DHRGPFGDRSELVFAIASASLWTLGLVLELTSVAEGAPVTAVFIASGLLGGYFTSREA
                     IESVRNGRFEIDFLMLVAAAGAAVLGKWEEGALLLALFSLGHALEGYATGRARRAIEA
                     LADLAPSTAVIRGAEGEREIPVDELAVGDTVVVKPNERIPADGFVTAGSSAVDQAPLT
                     GESIPVDKVPVLDRAAAQAAPDRVPASSRLFSGTINGAGQLEVTVTRLAADSTLSKLA
                     TMVREAETQASPTQRFTDRFERAFVPAVLVLVIAVAAVGGLVGSWSESSYRAMAVLVA
                     ASPCALAIATPSAVLAAVARAGQLGVLVKGGGPLENLGTLRAVAFDKTGTITEGRPRL
                     TDVIPAADVDEADLLAVSIAVERRSDHPLAAAVTRGGIERLGSDGGLDATRVTAVTGR
                     GVRAVVGSDEVLIGSAALFGEGGLPVEATDAITGLQARGRTVMVVKRAGAVLGVLGLM
                     DTPRPGARPAIESLEAVGIRRAVMLSGDHQLVAESIAREVGLDEAWGDLMPEDKVAAI
                     ERLRSTEGKVAMVGDGVNDAPALAHATVGIAMGAAGSDVALETADVALMGDDLAKLPV
                     VIGLSRKGSAVIRQNLFL"
BASE COUNT          297 a          856 c          868 g          319 t
ORIGIN      
        1 acgcccacgt ccgcctcctg ctcgacgtca gcctccagca cgtgggccac gggacctcgg
       61 tgccgtgagc gagaagctgc agctggagct ctcggtcctg ctgcccgatg ccctcgaccg
      121 ccgagatggc tgcgtccacc ggctcaccga gacgctcgac ggggccccgg gcgtcgacga
      181 cgtccacctg gtcgacggcg ccgaaggagg cacggcccgc ctgtgcctcc actacgaccc
      241 ggccgccacg agcctcagcc gcatccgggc ccgggccgag gcggcgggtg cggcgctgac
      301 cgagcgctac gcccacgtcc tctggccggt cgacggcatc ggccacatcc gcaaggcccg
      361 atcggtgggc gagtcgctga gccgggtcga cggggtgatg gaggcggatg tcgcccccgg
      421 gttcgtccgc gtcgagctcg accggacgaa gctcgacgag gatgccctcc gtgcggcgct
      481 ggccgaccgc aaggtgtcgg tcgccgacgg tcgcggcgac cacgagcacg ggaccggcgc
      541 gcacgaccac cgcggcccgt tcggcgatcg cagcgagctg gtcttcgcca tcgcctccgc
      601 ctcgctgtgg acgctgggcc tggtgctcga gctgacgtcg gtggcggagg gggcgccggt
      661 cacggcggtg ttcatcgcct cgggtctgct cggcgggtac ttcacgagca gggaggcgat
      721 cgagagcgtc cgcaacggcc gcttcgagat cgacttcttg atgctcgtcg ccgcggctgg
      781 tgccgccgtg ctgggcaagt gggaggaggg cgccctgctc ctcgccctct tcagcctcgg
      841 ccacgccctc gagggctacg cgacgggtcg cgcccgtcgg gccatcgaag ccctcgccga
      901 cctcgccccg tccacggccg tgatccgcgg cgccgagggg gaacgggaga tccccgtcga
      961 cgagctcgcc gtcggcgaca ccgtggtggt caagccgaac gagcgcatcc cggctgacgg
     1021 gttcgtcacg gcgggatcga gcgcggtcga ccaggctccg ctcaccggcg agagcatccc
     1081 cgtggacaag gtgccggtgc tcgatcgggc ggcggcgcag gctgcacccg accgggtgcc
     1141 cgcctcgtcc cgcctgttct cgggcacgat caacggtgcg gggcagctcg aggtgaccgt
     1201 gacccgcctg gccgcggact cgacgctctc gaagctggcc accatggtcc gcgaggcgga
     1261 gacccaggcg tcgcccacgc agcggttcac cgatcggttc gagcgggcgt tcgtgcccgc
     1321 ggtgctggtc ctggtcatcg ccgtggcggc ggtgggcggg ctggtcggat cgtggtcgga
     1381 gtcgtcctac cgggcgatgg ccgtgctcgt cgcggccagc ccctgcgcgc tggccatcgc
     1441 cacgccgagc gccgtgctgg cggccgtggc ccgggccggg cagctcggcg tgctggtcaa
     1501 aggcggcggg ccgctggaga acctcggcac gttgcgtgcc gtcgccttcg acaagaccgg
     1561 gacgatcacc gaggggcggc cgcgcctcac cgatgtgatc cccgccgccg acgtcgacga
     1621 agcagacctg ctcgcggtct cgatcgcggt cgagcgccgg agcgaccacc ccctcgcggc
     1681 cgccgtcacc cgaggcggca tcgagcgcct cggatccgac ggcgggctcg atgcgacccg
     1741 ggtcacggcc gtgaccggtc gtggcgtccg ggcggtcgtc gggagcgacg aggtgctgat
     1801 cggcagcgcc gccctcttcg gcgaaggcgg gctgcccgtc gaggcgaccg atgcgatcac
     1861 cggcctgcag gcccggggcc gcacggtcat ggtcgtcaag cgggccggcg cggtcctcgg
     1921 cgtcctcggt ctcatggaca cgccccgacc cggcgcccgc cccgcgatcg agtccctcga
     1981 agcggtcggc atccgccgcg ccgtcatgct ctcgggcgac caccagctcg tggccgagtc
     2041 catcgcccga gaggtcggcc tcgacgaggc gtggggcgac ctgatgcccg aggacaaggt
     2101 cgccgccatc gaacggctcc gctccaccga gggcaaggtg gcgatggtgg gcgacggcgt
     2161 caacgacgct cctgccctcg cgcacgccac ggtgggcatc gcgatggggg cggcggggtc
     2221 cgacgtggcc ctcgagacgg ccgatgtcgc gctgatgggc gacgacctcg ccaagctccc
     2281 ggtcgtgatc gggctcagcc gcaagggaag cgcggtcatc cgccagaacc tgttcctcag
//
LOCUS       DATMFS010000624         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_252084, whole genome shotgun sequence.
ACCESSION   DATMFS010000624 DATMFS010000000
VERSION     DATMFS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252084"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VNQ33_11950"
     CDS             <1..797
                     /locus_tag="VNQ33_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWJ98865.1"
                     /translation="GGPVPVTEEPDPAPDGADEPAAGSVEGGDGGLEEVPAAPVRQRW
                     LSTGQVIVLVAAVAFLVGAIAYVVGDDQGSADPLNDVDVGFMQDMGYHHDQAVQMALL
                     LLDKDDVDPNLTSFATEVVVGQRYEQGVFSATLDRFGHSSDVGDTVMGWMGEPQPTDT
                     MSGLATEEQMQELRDAEGEEAEALWIALMSEHHLAGLHMADYAARHGRDTTTVNLANA
                     IVKNQRSEILDYARYRRDHGLPIPKGFTDPTKDQRLNPLSFREHHD"
     gene            896..1663
                     /locus_tag="VNQ33_11955"
     CDS             896..1663
                     /locus_tag="VNQ33_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98866.1"
                     /translation="MDTTAKATSTTAPQDLVLRAEAAGESDVDGNYRQGIARSGDGWL
                     FTTNNAIYRTDAAFNQTDQLLDAIPADLAVQGYDHLGDPDVAEGLIWVPVERDDKDAG
                     QQVTARYDEETLEFVDAVTVEQHHNAFIGVDDDGTAYSADEFSDDAIVRYRIVDGKVE
                     AMDPLPLSQTVERIQGGDISGGALWLSTDDDHNGVYRVDLETGEVHDLGSTGHVDGEG
                     EGIDATDVDGSLLHVLVADAAIVPMWVVDLQVTSKPA"
     gene            2040..>2846
                     /locus_tag="VNQ33_11960"
     CDS             2040..>2846
                     /locus_tag="VNQ33_11960"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HWJ98867.1"
                     /translation="MTAKAVRSPRKPLGLAVVLLIVVAYLAVGAGRAEAGPVGLGTAT
                     SYGVLAASAVTSTSTVLDPTVVNGDLGVSPGTSVTGFPPGIVNGAQHRADAESLQAQT
                     DLVLAYDDAAGRTPVTSVATELGGTTLLPGNYTGATLGLTGTVTLDAKGDPDAVFVIK
                     SNETLITAEGSSVSLIGSANPCNVYWVVGSSATFGVNSDFVGTTMAMASITATTGADF
                     EGRLLARTGAVTLDTNTITRPVCDTLPAGSTTTAAPTTTAAPTTTAAPTTT"
BASE COUNT          467 a         1011 c          974 g          394 t
ORIGIN      
        1 caggaggacc cgtgccggtc accgaagagc cagatcccgc gcccgacggc gccgacgagc
       61 ccgctgcggg cagcgtcgag ggcggcgacg gcgggctcga ggaggtaccg gcggcgcccg
      121 tccggcagcg ttggctctcc acggggcagg tcatcgtgtt ggtggcggcc gtcgccttcc
      181 tcgtcggcgc catcgcctac gtcgtcggtg acgaccaggg gagcgcggac ccgctcaacg
      241 acgtcgacgt cggcttcatg caggacatgg ggtaccacca cgaccaggcg gtgcagatgg
      301 cgctcctgct gctcgacaag gacgacgtcg acccgaacct cacgtccttc gccaccgagg
      361 tggtggtcgg gcagcgctac gagcagggcg tcttctcggc cacgctcgac cgcttcgggc
      421 actcgtccga cgtcggcgac acggtcatgg gctggatggg cgaaccccag cccaccgaca
      481 ccatgtccgg cctcgccacc gaggagcaga tgcaggagct gcgcgatgcc gagggcgagg
      541 aggccgaggc gctctggatc gcgctcatgt ccgagcacca cctggcggga ctccacatgg
      601 cggactacgc cgctcgccac ggaagggaca cgaccacggt gaacctggcc aacgccatcg
      661 tgaagaacca gcgctccgag atcctcgact acgcccgcta ccggcgcgac cacgggctgc
      721 cgatcccgaa gggcttcacc gatccgacga aggaccagcg gctcaacccg ctgagcttcc
      781 gggagcacca cgactgatgg ggcgcagagc gagggtcgtc gctgccgcag cggtggcagc
      841 ggggctgctg gcctcgtgct cggggtcgag cggaggcggg tcggacgccg gcccggtgga
      901 cacgacggcg aaggccacct ccaccacggc gcctcaggac ctcgtcttgc gggcagaggc
      961 cgcaggggag tcagacgtcg acggcaacta ccggcagggc atcgcccggt ctggcgacgg
     1021 atggctcttc accaccaaca acgcgatcta ccgcaccgac gccgcgttca accagaccga
     1081 ccagctgctc gacgccatcc ctgccgacct ggcggtgcag ggctacgacc acctcggcga
     1141 ccctgacgtg gccgaggggc tcatctgggt gccggtcgag cgcgacgaca aggacgccgg
     1201 ccagcaggtc accgctcgct acgacgagga gacgctcgag ttcgtcgatg cggtcacggt
     1261 ggagcagcac cacaacgcgt tcatcggtgt cgatgacgac ggcaccgcct actcggccga
     1321 cgagttcagc gacgacgcga tcgtccgcta ccggatcgtc gacgggaagg tcgaagcgat
     1381 ggacccgctg ccgctcagcc agaccgtcga gcgcatccaa ggcggtgaca tcagcggtgg
     1441 ggcgctgtgg ctctccaccg acgacgacca caacggcgtc taccgcgtcg acctggagac
     1501 cggcgaggtg cacgacctcg gttcgactgg acacgtcgac ggcgaaggcg aggggatcga
     1561 cgccaccgac gtcgacggct ccctgctgca cgtcctggtc gccgacgccg ccatcgtccc
     1621 catgtgggtg gtggacctgc aggtcacgtc gaagccggcc tgagaccctg ggcgcgctcc
     1681 ccgtcgtggt gcggcccagc ccgaaccgcg acgaggagcg aagcgcagcg gtgaacctac
     1741 ggtccgcctc gcggccgtgg gtttcggccg ggtgaaccct ggctggggtc gatctgacgc
     1801 ctccggatcg cgggatcgac cgccgacgcg gcctcgcgtg ctgggccgcg aagcacaggg
     1861 aaccggagca tgttctcagg ggtttctctg tctttgggca tgttctgctg tgttcatcca
     1921 gcttcgatgt cttcagatgc ctcatgttca gcaccgatcg gccgaaggtg gaagggccgg
     1981 catctgaccg actgccttgg acccaaccgt tccgggacct tccacgaaag gcacatcccg
     2041 tgaccgccaa ggccgtccga tcccctcgca agcccctcgg gctcgccgtt gtcctgctca
     2101 tcgtcgtcgc ctacctcgcg gtcggcgccg ggcgcgccga ggccggaccg gtcgggttgg
     2161 gcacggccac gagctacggc gtgctggccg cctcggcggt caccagcacg agcacggtcc
     2221 tggacccgac ggtcgtgaac ggtgatctcg gcgtcagccc cgggacctcc gtcaccggct
     2281 tccctccagg catcgtcaac ggtgcacagc accgcgccga tgcggagtcg ctgcaggcgc
     2341 agaccgacct ggtgctggcg tacgacgacg cggccggccg taccccggtg acctccgtcg
     2401 cgaccgagtt gggcggcacg acgctgctcc cgggcaacta cacgggagcc acgctcgggt
     2461 tgaccggaac ggtcacgctc gacgccaagg gggatcctga cgccgtgttc gtgatcaagt
     2521 cgaacgagac cctgatcacc gctgagggca gcagcgtgtc gctcatcgga agcgccaacc
     2581 cgtgcaacgt gtactgggtc gtgggctcct cggccacgtt cggcgtgaac tcggacttcg
     2641 tcgggacgac catggcgatg gcatcgatca ccgccaccac cggcgccgac ttcgagggcc
     2701 gcctgctggc gcggaccggt gcggtcacgc tcgacaccaa cacgatcacc cgaccggtgt
     2761 gcgacacgct gccggcgggt tcgaccacca cggcggcgcc gaccaccacg gcggcgccga
     2821 ccaccacggc ggcgccgacc accacg
//
LOCUS       DATMFS010000625         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_229685, whole genome shotgun sequence.
ACCESSION   DATMFS010000625 DATMFS010000000
VERSION     DATMFS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229685"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1070)
                     /locus_tag="VNQ33_11965"
     CDS             complement(342..1070)
                     /locus_tag="VNQ33_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ98868.1"
                     /translation="MPKPTIVLVHGAWADGSSWNAVSTALQADGFTVQTPPNLLHGVA
                     SDSAYVASFLAQRTEGPVVLVGHSYGGVVITNAGLGGGDVKALVYVNAFIPDEGESVF
                     DILGGSGSALDVPDPTAVVDLAGYPDAPEGDVEAFLKPATVHESFAQELPEADRAVIA
                     ASQRPIALAANIGPTANPAWRSISSWAVLGTEDRVIPIATQRNMAERAGSTITEVAAS
                     HVSMVSQPQAAIDAIVAAAAAVEG"
     gene            complement(1192..2025)
                     /gene="fabI"
                     /locus_tag="VNQ33_11970"
     CDS             complement(1192..2025)
                     /gene="fabI"
                     /locus_tag="VNQ33_11970"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWJ98869.1"
                     /translation="MIGGRDPGGDVLLQDRTVLITGVLTPSSIAHTCARIAQEQGATV
                     VLTSFGRAMPTTRRAARKLPVEPEVLELDVTDQAALDALAPQVADRFGELHGVVHAVG
                     FAPAACLGQDEGLFAASWDEVATALQVSAWSLPALAKAVRPVLGRGGSIVGLDFDASV
                     AWPAYDWMGVAKAALESASRYLARDLGPEGIRVNLVAAGPLRTVAARSIPGFSQIDGV
                     WQERAPLGWDVDDPDPAARAVVALLSDWFPATTGEIVHVDGGVHAIGAAGPPAPTAAV
                     T"
     gene            complement(2046..>2443)
                     /locus_tag="VNQ33_11975"
     CDS             complement(2046..>2443)
                     /locus_tag="VNQ33_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98870.1"
                     /translation="EARSLASWTERTVALHETRSDSVTLGPDAWAVVVKLAGSADAGA
                     LRARLGWSPDRIAAALAEIEDRGVLAPSPPAPGASAAPAAPAPPPAPLPPPPTAAAGG
                     HHTGPLAPPPSSAVASHRRRGLPSRRASR"
BASE COUNT          309 a          846 c          977 g          311 t
ORIGIN      
        1 tcacgaccgc ggcacggacc gtcgggtcgg cctcgaggtg ggcgaacacc tcccggtact
       61 cggtgtgcat ccggccagtc cacgcgttca tgcggtccgg ccggtccagc gtgacgacgg
      121 cgacaccgcc gtcgcgctcg agccggatgg tgcggagctc gctcaggtcc atgggcgcat
      181 cgtgccactc ggtgggaggc tcggggctgt gcaggccctc ggcatccacc acgtgtccgt
      241 caacgcgacc gagccgtccg aggccggccc cggcctgcag ccgttcctgg acccgtccgg
      301 gaacgccgtc gagcgcgacg agcgcgttgc ccctgagggc gtcagccctc gacagcggcc
      361 gcggcggcga cgatggcgtc gatggcggct tggggctggg agaccatcga gacgtgggag
      421 gcggcgacct cggtgatggt ggacccggcg cgctcggcca tgttccgctg ggtggcgatc
      481 gggatcaccc ggtcctcggt gccgagcacg gcccagctcg agatggacct ccacgccggg
      541 ttcgcggtcg ggccgatgtt ggcggccagg gcgatgggcc gctggctggc ggcgatgacc
      601 gcccggtcgg cttcgggcag ttcctgggcg aacgactcgt gcaccgtcgc cggcttgagg
      661 aacgcctcga catcgccctc gggtgcatcc gggtacccgg cgaggtcgac caccgccgtg
      721 gggtcgggga cgtcgagcgc cgaacccgag ccgccgagga tgtcgaacac gctctcgcct
      781 tcgtcgggga tgaaggcgtt cacgtacacg agcgccttga cgtcgccgcc gccgaggccg
      841 gcgttggtga tgacgacgcc gccgtaggag tggccgacga gcacgaccgg gccttcggtg
      901 cgctgggcga ggaacgaggc gacgtaggcg ctgtcgctgg cgacgccgtg gagcaggttc
      961 ggcggcgtct gcacggtgaa gccgtcggcc tggagcgcgg tggacacggc gttccagctg
     1021 gagccgtcgg cccaggctcc gtggacgagg acgatggtgg gcttcggcat ggggtgcctc
     1081 ctggtcgtcg gccgcccgca cggcctgctc cggaggctac cgggcgatcg tccgccgtgg
     1141 cggcaagatc tgacggtccg tcaggcagcg gacaggtgag cgcgggccgc gctaggtgac
     1201 ggcggcggtc ggggcgggtg gaccggcggc accgatggcg tgcacgccgc cgtcgacgtg
     1261 gacgatctcg cccgtggtcg cggggaacca gtcgctcagc agggcgacca ccgcccgggc
     1321 cgccggatcg gggtcatcga cgtcccatcc cagcggggcc cgctcttgcc acaccccgtc
     1381 gatctggctg aacccgggga tgctgcgggc ggcgaccgtg cgcagcggcc ccgcggccac
     1441 gaggttgacc cggatgccct cggggccgag gtcgcgggcg aggtagcgac tggcggactc
     1501 cagcgccgct ttggccacgc ccatccagtc gtaggccggc caggcgaccg aggcgtcgaa
     1561 gtcgaggccg acgatcgacc cgccgcgccc gagcaccggg cgcaccgcct tggccagggc
     1621 gggcagcgac cacgccgaga cctggagggc ggtggcgacc tcgtcccacg aggcggcgaa
     1681 caggccctcg tcctggccga ggcaggcggc gggcgcgaac ccgacggcgt ggacgacgcc
     1741 gtggagctcg ccgaagcggt cggcgacctg cggggccagg gcgtcgaggg ccgcctggtc
     1801 ggtgacgtcg agctcgagca cctcaggctc gaccggcagc ttgcgagcgg ctcggcgcgt
     1861 ggtcggcatc gcccggccga acgacgtcag cacgaccgtc gccccctgct cctgggcgat
     1921 gcgggcgcag gtgtgggcga tcgacgaggg cgtcagcacc ccggtgatca gcaccgttcg
     1981 atcttgcagc agcacgtcgc ctccggggtc gcgtcccccg atcatgccgg aacggcggtg
     2041 gcaggtcagc gactggcgcg ccgagacggc aggccccgcc gtcgatggga ggcgacggcg
     2101 ctgctgggcg gtggggcgag cggaccggtg tggtgcccgc cggccgccgc agtcggtggc
     2161 ggtggcagcg gggccggagg cggcgccgga gcggcaggcg ctgctgacgc gcccggcgct
     2221 ggcggtgacg gggcgagcac cccgcggtcc tcgatctcgg cgagggcggc ggcgatgcgg
     2281 tccgggctcc accccaggcg ggcgcgcagc gcaccggcgt cggccgaacc cgccagcttg
     2341 acgacgaccg cccaggcgtc gggcccgagg gtgacgctgt ctgaccgggt ctcgtgcagg
     2401 gccaccgtgc gctcggtcca cgaggcgagg ctgcgtgcct cgt
//
LOCUS       DATMFS010000626         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_137992, whole genome shotgun sequence.
ACCESSION   DATMFS010000626 DATMFS010000000
VERSION     DATMFS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137992"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNQ33_11980"
     CDS             complement(<1..272)
                     /locus_tag="VNQ33_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HWJ98871.1"
                     /translation="MDNVRKSFQQTLEEIRQEMVQMAALVTEGIPRATEALLAGDLTD
                     AQRIIEGDDPLDARAVELEEVCYQQLALQQPMAGDLRGIVTAIRMIS"
     gene            complement(289..951)
                     /gene="phoU"
                     /locus_tag="VNQ33_11985"
     CDS             complement(289..951)
                     /gene="phoU"
                     /locus_tag="VNQ33_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWJ98872.1"
                     /translation="MVRAGFNAELEQLRLQVEVMAVRVDQNLERMKDFLAAGDLEVAA
                     VTVAADDDIDAMNLSLTERCYDLLARESPVASDLRFVMSVLRVLGELERVGDLALRVV
                     KVGEDWSLVRASASTYDILVSMAETSVEQFREALRAWSSQDLDLADHLARSGPVLDVA
                     NAQLVRELLLLEGPSAVATALHSLVIGKSLDRIADHSAIIGSRLRYLLTGDPDHLAAE
                     IR"
     gene            complement(960..1847)
                     /gene="pstB"
                     /locus_tag="VNQ33_11990"
     CDS             complement(960..1847)
                     /gene="pstB"
                     /locus_tag="VNQ33_11990"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWJ98873.1"
                     /translation="MTEEETPVPQPAPTPLSDLAHETAEEHPPPAASAGATVFDVDDL
                     SVLYSGFRAVKDVDISITKHEITAFIGPSGCGKTTVLRCFNRMNDLIEGAAVEGSLKY
                     HGVDLYGKDVNPVEVRRRIGMVFQKPNPFPKSIFENVAYGPKLAGIKKKSELDGIVEQ
                     ALRGAAIWDEVKDRLHKSGLGLSGGQQQRLCIARALAVNPEVILMDEPCSALDPIATG
                     RIEDLMEEIKQDYTIIIVTHNMQQAARVSDRCAFFTTEVNPDSDQRTGTLVEFDATEK
                     MFSNPSDERTENYVTGRFG"
     gene            complement(1859..>2095)
                     /locus_tag="VNQ33_11995"
     CDS             complement(1859..>2095)
                     /locus_tag="VNQ33_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98874.1"
                     /translation="AAGETAPVLFTIGAINSVAAAKTITGQSTTLSVQIYNMITNGGK
                     LSPQMAWGAAFTLIAIVLILTSIARAVSARFVMR"
BASE COUNT          306 a          675 c          752 g          362 t
ORIGIN      
        1 gagatcatgc ggatcgcggt gacgatgcca cggaggtcgc cggccatcgg ctgctggagg
       61 gccagctgct ggtagcagac ctcctcgagc tccacggccc gggcatcgag cgggtcgtcg
      121 ccctcgatga tgcgctgggc atcggtgagg tcgccggcca gcagggcctc ggtggcgcgg
      181 gggatgccct cggtcaccag ggcggccatc tgcaccatct cctggcggat ctcctccagc
      241 gtctgctgga agctcttgcg aacgttgtcc atttggtcct gtcccgggtc agcggatctc
      301 ggcggcgagg tggtcggggt cgccggtcag caggtaccgg aggcggctgc cgatgatggc
      361 ggagtgatcg gcgatccgat cgagcgactt cccgatcacc aggctatgga gcgcggtggc
      421 caccgccgac ggaccctcca ggagcaggag ctcgcgcacc agctgggcgt tggccacgtc
      481 gagcaccgga cccgaccgtg cgaggtggtc ggcgaggtcg aggtcctggc tggaccaggc
      541 tcgcagcgcc tctcggaact gctcgacgga cgtctcggcc atcgacacca ggatgtcgta
      601 ggtggaggcc gatgctcgga cgagcgacca gtcctccccc accttcacga cgcgcagcgc
      661 cagatcgccg acgcgctcga gctccccgag cacccgcagg accgacatca cgaaccggag
      721 gtccgaggcc acgggcgact ctcgggcgag caggtcgtag cagcgctcgg tgagcgacag
      781 gttcatggcg tcgatgtcgt cgtcggccgc gacggtcacc gccgccacct cgagatcgcc
      841 ggccgccagg aagtccttca tccgctcgag gttctgatcg acccggacgg ccatcacctc
      901 gacctgcagc cgcagctgct cgagctctgc gttgaacccc gcgcggacca tcggcagcct
      961 cagccgaacc ggccggtgac gtagttctcg gtccgctcgt cggaggggtt cgagaacatc
     1021 ttctcggtcg cgtcgaactc cacgagggtg ccggtgcgct ggtcgctgtc ggggttgacc
     1081 tccgtggtga agaaggcgca gcggtcgctc acccgtgccg cctgctgcat gttgtgggtc
     1141 acgatgatga tcgtgtagtc ctgcttgatc tcctccatga ggtcctcgat gcgcccggtg
     1201 gcgatcgggt cgagggcgga gcagggctcg tccatcaaga tgacctcggg gttcaccgcc
     1261 agggcccggg cgatgcacag ccgctgctgc tggccgccgg agaggccgag gccggacttg
     1321 tggagccggt ctttgacctc gtcccagatg gcggcgcccc gcagcgcctg ttcgacgatg
     1381 ccgtcgagct cgctcttctt cttgatgccg gcgagcttcg ggccgtaggc cacgttctcg
     1441 aagatcgact tcgggaacgg gttcggcttc tggaacacca tgccgatgcg gcggcgcacc
     1501 tcgacggggt tcacgtcctt cccgtacagg tcgacgccgt ggtacttgag cgacccctcg
     1561 accgccgccc cctcgatcag gtcgttcatg cggttgaagc agcgcagcac ggtggtcttg
     1621 ccgcagcccg acgggccgat gaaggcggtg atctcgtgct tggtgatcga gatgtcgacg
     1681 tctttgacgg cgcggaagcc gctgtagagg accgagaggt cgtcgacatc gaagaccgtc
     1741 gcgccggcgg acgcggccgg tggcgggtgc tcctcggcgg tctcgtgggc gaggtccgag
     1801 agcggggtgg gggccggttg gggcacgggg gtctcctctt cggtcatggg aggcagtctc
     1861 accgcatcac gaaccgtgcg ctgacggccc gggcgatgct ggtcaagatg agcacgatcg
     1921 cgatgagcgt gaaggccgcg ccccatgcca tctggggcga gagcttcccg ccgttggtga
     1981 tcatgttgta gatctgcacc gagagcgtgg tgctctggcc ggtgatggtc ttggccgccg
     2041 ccaccgagtt gatggcgccg atggtgaaca gcacgggagc ggtctcgccg gcggc
//
LOCUS       DATMFS010000627         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_67397, whole genome shotgun sequence.
ACCESSION   DATMFS010000627 DATMFS010000000
VERSION     DATMFS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67397"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VNQ33_12000"
     CDS             complement(<1..290)
                     /locus_tag="VNQ33_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98875.1"
                     /translation="MTTTDAPLVTLTVPADVRYFRSVRLAVGGLATMVGFDIEAIEDL
                     RIGVDEVCAALAEAGNGDDLRIEVLAVPDRVLRVEGTVPHGDEQPDEDRFTFS"
     gene            501..692
                     /locus_tag="VNQ33_12005"
     CDS             501..692
                     /locus_tag="VNQ33_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ98876.1"
                     /translation="MGSNADDAKGRVKEAVGDVTGDRDLEREGKLDQAGASVKEFAEN
                     AKDKAADLVDSVKDKLNKD"
     gene            complement(782..1237)
                     /locus_tag="VNQ33_12010"
     CDS             complement(782..1237)
                     /locus_tag="VNQ33_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ98877.1"
                     /translation="MTNKASRQPLEHPRILLVEDDPGDVLITRESLEASRAGCEIDVV
                     DDGAMALDFLHKRRGYEDAPTPHLVLLDLNLPKVSGHQVLAELKADPDLAPIPVVVLS
                     TSTADDDVHATYALHGNAHVAKPVDLERYQDAVRAIDEFFLGVAEAPLA"
     gene            complement(1248..>2522)
                     /locus_tag="VNQ33_12015"
     CDS             complement(1248..>2522)
                     /locus_tag="VNQ33_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ98878.1"
                     /translation="VATLDRVESAMDDWRDSVADPLLERPVDKRDLDGTFIRSTAVFD
                     EVRAELDNLEAVLQRERDIARTNLEDTTSRLVVSVGATIVLFGTVAFGGSWILRRRVV
                     APVDEVVQAADAVAGGDLDVPIAVRGPAEIELLAQRVSVMRDRIVEELAAVEASRVEL
                     ARRAEELARSNADLEQFAYVASHDLQEPLRKVASFCQLLEQRYGDELDERGRSYIEFA
                     VDGAKRMQALIADLLEFSRTGRSTQAFVACDFGVLARDAIGGYADQLAGASVTVGALP
                     TLPGDPTLYRALLDNLIGNAIKYRAGEDPPVISLSAERDGSEWVFTCSDHGIGIEPPY
                     REQVFAVFQRLHGRSEFEGTGIGLALCKRIVEFAGGRIWIADPVDGIGTTVRWTLPTG
                     RRDGPVGAPATTDVPPAPGGGRDPDLTRLPTP"
BASE COUNT          386 a          854 c          943 g          339 t
ORIGIN      
        1 gagaaggtga agcggtcctc gtcgggctgc tcgtcgccat ggggcacggt gccttcgacg
       61 cgcagcacgc ggtcgggcac cgcgaggacc tcgatccgga ggtcgtcgcc gttgccggcc
      121 tcggccagtg ccgcgcacac ctcgtcgacg ccgatgcgga ggtcctcgat ggcttcgatg
      181 tcgaacccga ccatcgtcgc cagaccgccg accgccaggc ggaccgagcg gaagtagcgc
      241 acgtcggccg gcacggtcag ggtcaccagc ggagcatcgg tcgtggtcat gcggtcatcc
      301 tcgcgccgaa gcgccgggac ctggggatga tctgggggca tgccgggcat ccatctccgc
      361 tcgttcgggc cggtcggcca ggtggaacca cctacccctg aggtcccggt atcagaccgg
      421 gcggcaccgg gtagcaggag ggcacgagcg gccgatgcac ccggcgctca ccgatcgcaa
      481 cgcaacagag gagcaagcac atgggcagca acgccgacga tgccaagggc cgagtcaaag
      541 aggcggtcgg ggacgtgacc ggcgaccgcg acctcgagcg ggaaggaaag ctcgaccaag
      601 ccggcgccag cgtgaaggag ttcgccgaga acgccaagga caaggcggcc gacctcgtgg
      661 actccgtcaa ggacaagctc aacaaggact gatcacccac cgcaccaggc ggcgaggctc
      721 ccgtgacgac ccgccggtcg cgggagcctc gtcgggtctg ggcagccccc ggcggcgcac
      781 ctcaggcgag cggcgcttcg gccacgccga ggaagaactc gtcgatggcg cgcacggcgt
      841 cctggtagcg ctcgaggtcg accggcttgg ccacatgggc gttgccgtgg agcgcgtacg
      901 tcgcgtgcac gtcgtcgtcg gccgtactcg tgctgagcac caccaccggg atgggagcga
      961 ggtcggggtc ggccttcaac tcggcgagga cctggtggcc ggacaccttg ggcaggttca
     1021 agtcgagcag cacgaggtgc ggggtcggcg cgtcctcgta gcccctccgc ttgtggagga
     1081 agtcgagcgc catggcgccg tcatcgacca cgtcgatctc gcagcccgcc cgcgaggcct
     1141 ccagggactc ccgggtgatg agcacatcgc ccgggtcgtc ctccacgagg aggatgcggg
     1201 ggtgctcgag cggttggcgg gatgccttgt tggtcatcga ggtccagtca tggggtgggg
     1261 agacgggtga gatcggggtc gcgcccgccg ccgggcgcag gggggacgtc cgtcgtggcg
     1321 ggcgcgccca cgggaccgtc gcggcgtccg gtgggcagcg tccagcggac ggtcgtgccg
     1381 atgccgtcga cggggtcggc gatccagatg cggccgccgg cgaactcgac gatgcgcttg
     1441 cacagtgcca ggccgatccc ggtgccctcg aactcgctgc ggccgtgcag ccgctggaac
     1501 acggcgaaga cctgctcgcg gtacgggggc tcgatgccga tgccgtgatc cgagcaggtg
     1561 aacacccact cgctgccgtc gcgctcggcc gacagcgaga tcacgggcgg atcctcgccg
     1621 gcgcggtact tgatggcgtt gccgatcagg ttgtcgagca gggcccggta gagggtcggg
     1681 tcgcccggga gggtcgggag cgcaccgacg gtcacgctcg cgccggcgag ctggtcggcg
     1741 tagccgccga tcgcgtcgcg ggcgagcacg ccgaagtcgc acgcgacgaa cgcctgggtg
     1801 gagcgtcccg tgcgggagaa ctcgagcagg tcggcgatca gcgcctgcat gcgcttggcc
     1861 ccgtcgaccg cgaactcgat gtaggagcgt ccccgctcgt cgagctcgtc gccgtagcgc
     1921 tgctcgagga gctggcagaa cgacgccacc ttccgcagcg gctcctgcag atcgtgcgac
     1981 gcgacgtagg cgaactgctc caggtccgcg ttggagcggg cgagctcctc ggcgcgccgg
     2041 gccagctcca cgcgcgaggc ctcgaccgcg gcgagctcct cgacgatgcg gtcgcgcatg
     2101 accgagacgc gctgggcgag cagctcgatc tccgcgggac cccggacggc gatgggcacg
     2161 tcgaggtcgc cgcccgccac ggcgtcggcc gcctgcacga cctcgtcgac cggcgcgacc
     2221 acccggcggc gcaggatcca gctgccgccg aacgccacgg ttccgaacag gacgatggtg
     2281 gcgccgaccg acacgacgag gcgcgaggtg gtgtcctcga ggttggtccg ggcgatgtcg
     2341 cgctcccgct ggaggacggc ttcgaggttg tcgagctcgg cccggacctc gtcgaagacg
     2401 gccgtgctcc ggatgaacgt gccgtcgagg tcgcgcttgt cgaccggcct ctcgagcagc
     2461 ggatcagcca cgctgtcgcg ccagtcgtcc atggctgact ccacccggtc gagcgtcgcc
     2521 ac
//
LOCUS       DATMFS010000628         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_45294, whole genome shotgun sequence.
ACCESSION   DATMFS010000628 DATMFS010000000
VERSION     DATMFS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45294"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNQ33_12020"
     CDS             complement(<1..432)
                     /locus_tag="VNQ33_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98879.1"
                     /translation="MVMGRREDVETASAPIAAFAVAGMLEQMALAVLSVPFRSPWKGS
                     HSVGDNIGASVTRQVMRSFMGYSTSIPTEELRSIEVLIDDLCRVVMPPVARSLDVTMD
                     HAEINGIPGLWYHPDQGTEPSPKGTVLYLHGGGYVGTCPTMY"
     gene            complement(498..1076)
                     /locus_tag="VNQ33_12025"
     CDS             complement(498..1076)
                     /locus_tag="VNQ33_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWJ98880.1"
                     /translation="MQANREEEPVDARVRRRLRELRAEQGLTLQQVAERAHLDVSTLS
                     RLEAGKRRLALDHLPGLAAALGVTADELISDRPPFDPRVHSEPRTQDGLTMWPLTNRP
                     SHGGQHAFKVLIDPRRDTPPDPLPVHEGYDWMYVLHGRMRLCLGEEELVIEPGEAVEF
                     STLTPHWFGTVAGPVEVLALFGPHGQRQHLHA"
     gene            1124..2056
                     /locus_tag="VNQ33_12030"
     CDS             1124..2056
                     /locus_tag="VNQ33_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ98881.1"
                     /translation="MDEIWDCIVVGGGAAGLSAALVLGRARRRTLVVDAGKPSNAVAH
                     GIGGLLGHDGRPPAELYAMGRAEIAAYPTVEVRSGEVLAGETAGDHFALTLDDGRIET
                     AKRVLLASGMDYERPAVPGVEERWGRSVFHCPFCHGWEVREKKLGVLDGGPMGAMRAL
                     MLTMWSDDVTLYTDGPAALEPVDAHRLHSAGIAVVEQPVTRLLGPGDQLEGVELADGS
                     TRACGGLLLAVTLRQRSPLADQLGAEAAPASPIAADALAIDAMSMTSVPGLFAAGDLG
                     ATMPSVANAVAAGSNAAAAVVHSLIADRHPLPAG"
BASE COUNT          291 a          800 c          757 g          297 t
ORIGIN      
        1 gtacatcgtg gggcacgtgc cgacgtagcc gccgccgtgg aggtagagga cggtaccctt
       61 cggcgacggc tcggtgccct ggtccgggtg gtaccagagg ccggggatgc cgttgatctc
      121 ggcgtggtcc atggtgacgt cgagcgaccg ggccacgggc ggcatgacca cccggcacag
      181 atcgtcgatc agcacctcga tcgagcggag ctcctcggtc gggatcgagg tcgagtaccc
      241 catgaagctg cgcatgacct ggcgggtcac gctcgccccg atgttgtcgc cgacggagtg
      301 cgagcccttc cagggcgaac ggaacggcac gctcagcacc gccagcgcca tctgctcgag
      361 catccccgcc accgcgaacg ccgcgatcgg cgccgatgcg gtctccacgt cctcgcgtcg
      421 ccccatgacc atgggcggtc aggctacggc agcacccacc tgccgcccgg ccgaacaccg
      481 ggcccggatc gcccggctca ggcgtggagg tgctggcgct ggccgtgcgg gccgaacagg
      541 gcgagcacct ccacagggcc ggcgaccgtg ccgaaccagt gcggcgtgag cgtcgagaac
      601 tcgaccgcct cgcctggctc gatcacgagc tcctcctcgc ccaagcacag ccgcatccga
      661 ccgtgcagca cgtacatcca gtcgtagccc tcgtgcaccg gcaacggatc gggcggcgtg
      721 tcgcgccggg ggtcgatgag caccttgaac gcgtgctggc cgccgtgcga cggacggttc
      781 gtgagcggcc acatggtgag cccgtcctgg gtgcgcggct cgctgtgcac ccgcgggtcg
      841 aagggcggcc ggtcgctgat cagctcgtca gcggtgacac cgagcgcggc agcgagaccg
      901 gggaggtggt cgagcgcgag gcgacgcttc ccggcctcga gccggctcag cgtggacacg
      961 tcgaggtgcg cccgctcggc cacctgctgg agggtcaggc cctgttcggc ccggagctcc
     1021 cgcagccgcc gtcgcacccg ggcgtcgacg ggctcctcct cgcgatttgc ctgcatggca
     1081 aacaaggttg ccacatcgat caccgtcgtg caccctcgac cgcatggacg agatctggga
     1141 ttgcatcgtg gtcggaggag gcgccgccgg gctcagcgcg gcgctcgtgc tggggcgggc
     1201 gcgccgccgc accctcgtgg tcgacgccgg gaagccgagc aacgccgtcg cccacggcat
     1261 cggcggcctg ctcggccacg acggccggcc cccggccgag ctctacgcca tgggccgagc
     1321 cgagatcgcc gcctacccga ccgtggaggt gcgcagcggg gaggttctcg ccggcgagac
     1381 cgcgggcgac cacttcgccc tgaccctcga cgacggccgc atcgagacgg ccaagcgggt
     1441 gctgctggca tcgggcatgg actacgagcg accggcggtc cccggcgtcg aggagcggtg
     1501 gggccgttcg gtcttccact gcccgttctg ccacgggtgg gaggtgcgcg agaagaagct
     1561 cggcgtcctc gacggtggtc cgatgggcgc gatgcgcgcc ttgatgctca cgatgtggag
     1621 cgatgacgtc accctctaca ccgacggacc cgccgccctg gagccggtcg atgcccaccg
     1681 cctccactcc gccggcatcg cggtcgtcga gcagccggtc acccgcctgc tcggccccgg
     1741 cgaccagctc gaaggcgtcg agctggccga cggctccacc cgggcgtgcg gcggcctgct
     1801 cctcgccgtg accctccgcc agcgttcgcc gctcgccgac cagctcggcg ccgaagccgc
     1861 ccccgcctcc cccatcgccg ccgacgccct ggcgatcgac gccatgtcga tgacctcggt
     1921 gcccggcctg ttcgccgccg gcgatctcgg ggcgacgatg ccgtcggtcg ccaacgccgt
     1981 ggccgccggc tcgaacgccg cggccgcggt ggtccacagc ctcatcgccg accgccaccc
     2041 gctcccagcc ggctgacggc gaaccggttc agtcctggac ggatgccgcg atcgggctga
     2101 ggaacttcgc ctcgtcccac tcgtcgggcg gcttggtgcc cttgc
//
LOCUS       DATMFS010000629         4700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_67420, whole genome shotgun sequence.
ACCESSION   DATMFS010000629 DATMFS010000000
VERSION     DATMFS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67420"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VNQ33_12035"
     CDS             <1..218
                     /locus_tag="VNQ33_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HWJ98882.1"
                     /translation="KRRGEIDGVEQLTRYLEFLNRDPMLRPVRGMFVAQQIKPQAKVL
                     AKDRGIDWVEVDYDELRGLESNELRLF"
     gene            220..993
                     /locus_tag="VNQ33_12040"
     CDS             220..993
                     /locus_tag="VNQ33_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HWJ98883.1"
                     /translation="MAGPGLLPGGAQPPSGFEPWCHTLRGEQAWSHVVPGGAPALATW
                     RALRDRHQRTGLWPVLLGSDPQVIDQLTWRLEMLARSKPGIPDVEDAPSAPELLARWM
                     GDPTEDEYLEDVPRHLAKLEQDADAIRARLTARPDDEAVRALAGDTVTIALIPAHGGW
                     EVPALLAWSGAERNDIGGPEHLAVLHFWHEVYGAELVALGLERMECLVTRPPEDARAA
                     FELAVQHYAYCPELMDNLVPAMGALAATLFGRHWLFDWT"
     gene            complement(1081..3108)
                     /locus_tag="VNQ33_12045"
     CDS             complement(1081..3108)
                     /locus_tag="VNQ33_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98884.1"
                     /translation="MTTTRAVRRSMAGLALGVAGVLVASAAAPAASAGSAGPGDARAR
                     RPVTASDARVAPEDVKPGEMAYLRAKSTPATGYVAARDVTGTDRVSSVKSTIVVTYHG
                     FPSNAKASFQRAVDLWSILVKSPVPIRIDATWKSLPSGVLGGARATDYYRNFSGAPKS
                     NTWYPIALANARAGRDLAANESDVTAQFASGRSDWYFGTDGQVPAGKLDFTSVVLHEI
                     GHGLGMTGGAGVSGSVGYWGLGESVPDVYDLFAQSASGTPITSYANPSTALRSVITSN
                     GMRWGGSQGTAANGGVKPYLYSPNPYNPGSSIYHLDEAAYGTGTPNALMTPYLDDGEA
                     LHDPGDIVLGMLRDLGWTTVGARGVPAAPVLKTAIGGPQKVIVSWTPALDTGRQFLTG
                     FRIYRYDDGGTTAAATYDVAADLTASTVTGLANGTTYRFAVAAKNASGVSAPSAKSAG
                     IKPVAMAPYTRTDVLVRQQFLDFHKRQPTAAESLKWIADLHSGAKSPLQTVTGIAKLH
                     GSYDVSTRTTRLYSGYFERLPDSSGYGYWTGRLRSGSSLKKVSDTFAASSEFKNKYGP
                     LSNTAFVKKVYANVLHRSPDSGGLSFWVNRLNKKVASRGDVMLNFSESNENVTKMDSE
                     VTSVLLRAAMLGRMPNPTEYAADIASLDGTGTLDTLAQAIMASPEYDARVP"
     gene            complement(3305..4513)
                     /locus_tag="VNQ33_12050"
     CDS             complement(3305..4513)
                     /locus_tag="VNQ33_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ98885.1"
                     /translation="MSVNRPTAPELTAPLADEAFYAGDPFPLYARLREEAPIVWHQPD
                     DGAGWWVVSTQPEVLEVSRDPSRFQSGKGILTMEIGIEYPSPPTMMHTDPPVHTRYRK
                     LVQPGFSPSRIRALEAPLRALVTRLLDELPTGEPVDIVPALAEPYPVHVIADLLGIAD
                     EDRDRFVLWSDATIPGASDLGFEARMALMAEMQEYLMAAGRDRRGADGTDLISVLANV
                     EIDGETLTDDELLMFFNQLLVAGNETTRNMISGGLWALASTPDQLARLVADRELIPSC
                     IEEWLRWTTPVVAFMRTATVDTELRGVPIAAGDPVLLLYAAANRDEEVFGADAGEFRV
                     DREPNPQVALGFGPHFCIGASLARLEGRILLEEVLQRWTAIEPAGDVVRTGSAVIAGL
                     KAAPLVLRSS"
BASE COUNT          648 a         1644 c         1719 g          689 t
ORIGIN      
        1 tcaagcggcg cggcgagatc gacggcgtcg agcagctcac ccgctacctg gagttcctca
       61 accgcgaccc gatgctccgg cccgttcgcg gcatgttcgt cgcccagcag atcaagcccc
      121 aagcgaaggt cctggccaaa gaccgcggca tcgactgggt cgaggtcgac tacgacgagc
      181 tgcgcgggct cgagtccaac gagctccggc tcttctgacg tggcaggacc cggactgctc
      241 cccggcggcg cccagccgcc ctccggcttc gagccctggt gccacacgct gcgcggcgag
      301 caggcgtgga gccacgtggt gccgggtggt gcgcctgccc tcgccacgtg gcgcgccctg
      361 cgcgatcgcc accagcgcac gggcctctgg cctgtgctgc tcggcagcga cccccaggtc
      421 atcgaccagc tcacctggcg cctggagatg ctcgcccgct cgaagcccgg gatccccgat
      481 gtcgaagatg cgcccagcgc gccggagctc ctcgcccggt ggatgggcga cccgaccgag
      541 gacgagtacc tcgaggacgt cccccgccac ctcgccaagc tcgagcagga cgccgacgcc
      601 atccgggcgc ggctcaccgc ccgccccgac gacgaggcgg tacgggccct cgccggcgac
      661 acggtgacga tcgcgctgat cccggcgcac ggcggctggg aggtgccggc gctgctcgcg
      721 tggtccggtg ccgagcgcaa cgacatcggc ggcccggagc acctggcggt cctgcacttc
      781 tggcacgagg tctacggcgc cgagctggtg gccctcggcc tcgagcgcat ggagtgcctc
      841 gtgacccggc cgccggagga cgctcgcgcc gcgttcgagc tcgcggtcca gcactacgcc
      901 tactgccccg agctgatgga caacctcgtc ccggcgatgg gcgccctggc cgccaccctc
      961 ttcggccggc actggctctt cgattggacc tagcgccgtg accggcgacc ggatccgccg
     1021 ggccacctga tcagacggcg ccggaccgga cgtcggggga cgccgtcggc ggaggcccgc
     1081 ctacggcacc cgggcgtcgt actcggggct ggccatgatg gcctgggcca gggtgtcgag
     1141 cgtccccgtc ccatcgagcg aggcgatgtc ggcggcgtac tcggtcgggt tcggcatgcg
     1201 gccgagcatc gcggcccgga gcaggaccga ggtgacctcg gagtccatct tggtgacgtt
     1261 ctcgttggac tcggagaagt tcaacatgac gtcgccgcgg ctcgcgacct tcttgttgag
     1321 ccggttcacc cagaagctca agccgccgct atcggggctg cggtgcaaga cgttggcgta
     1381 gaccttcttc acgaacgccg tgttggagag cggcccgtac ttgttcttga actccgagga
     1441 cgccgcgaac gtgtccgaca ccttcttcag ggatgacccg ctccgcagcc tgcccgtcca
     1501 gtacccgtag ccgctggagt cgggcagtcg ctcgaagtag cccgagtaga ggcgggtcgt
     1561 gcgggtgctg acgtcgtagg agccgtgcag cttggcgatc ccggtcaccg tctggagcgg
     1621 cgacttggca ccggagtgca ggtcggcgat ccacttcagc gactcggccg ccgtgggctg
     1681 gcgcttgtgg aagtcgagga actgctggcg gaccagcacg tcggtgcggg tgtagggcgc
     1741 catggcgacc ggcttgatgc cggcgctctt ggccgacggg gcgctcaccc ccgacgcgtt
     1801 cttcgcggcc acggcgaacc gataggtcgt gccgttggcc agcccggtga cggtgctggc
     1861 cgtcaggtcg gcagccacgt cgtaggtggc cgccgcggtg gtcccgccgt cgtcgtagcg
     1921 gtagatgcgg aagccggtga ggaactggcg gccagtgtcg agggccggtg tccacgagac
     1981 gatgaccttc tgcggcccgc cgatcgccgt cttcagcacc ggagcagccg ggacgcccct
     2041 ggcgcccacc gtggtccagc cgaggtcgcg cagcatgccg aggacgatgt cgccgggatc
     2101 gtggagcgcc tcgccgtcat cgaggtacgg cgtcatcagc gcgttggggg tgccggtgcc
     2161 gtaggcggcc tcgtcgaggt ggtagatcga cgatcccggg ttgtacgggt tgggcgagta
     2221 caggtacggc ttgaccccgc cgttggcggc ggtgccctgg gagccgcccc accgcatgcc
     2281 gttgctggtg atcacggagc ggagggcggt cgaagggttc gcgtagctgg tgatcggcgt
     2341 gccgctcgca ctctgggcga agaggtcgta gacgtcgggg accgactcgc ccaggcccca
     2401 gtagccgacg cttccgctga cgccggcacc tccggtcatg ccgaggccgt gaccgatctc
     2461 gtgcagcacg acgctcgtga agtcgagctt gccggcgggg acctggccgt cggtgccgaa
     2521 gtaccagtcc gagcggccgc tcgcgaactg cgcggtcaca tcggactcgt tggcggcgag
     2581 gtccctgccg gctcgggcgt tggccagggc gatggggtac cacgtgttgc tcttcggtgc
     2641 cccggagaag ttgcggtagt agtcggtggc cctggcgccg ccgaggacac cgctgggcag
     2701 gctcttccag gtggcgtcga tgcggatcgg caccggcgac ttgacgagga tcgaccacag
     2761 gtcgacggcc cgctggaacg aggccttggc gttcgacggg aagccgtggt acgtcacgac
     2821 gatggtcgac ttcaccgagc tgactcggtc ggtcccggtc acatcccgtg ccgccacgta
     2881 gccggtggcg ggggtcgact tggcccgcag gtaggccatc tcgccgggct tgacgtcctc
     2941 cggcgcgacc cgggcgtcgg aggcggtcac cggccgacgg gcccgagcgt ctcccggacc
     3001 ggccgagccg gccgaggcgg ccggcgcagc ggccgaggcg accagcaccc cggccacgcc
     3061 gagggcgagg ccggccatcg agcggcgaac cgcacgggtc gtcgtcatgt cgtgtacccc
     3121 cgcccggacc gggctcgtct ccgcgggcgg ccccgtgtgg gccgccaccg ccgtcgcacc
     3181 accatcggca ccgtggcggc cggtcttgac cctcgatcgc acgttgggtg aacgggcccc
     3241 gtcatcgacg gggtcccggc gacgcggtgc tgccgcggtc gcgtccccgc gaccgaccca
     3301 ccgctcagga ggatcgcagc accagcggtg ccgccttgag cccggcgatg acagctgagc
     3361 cggtgcgcac cacgtccccc gccggctcga tggccgtcca gcgctgcagc acctcctcga
     3421 ggaggatgcg gccctcgagc cgggccagcg aggcaccgat gcagaagtgg ggaccgaacc
     3481 ccagcgccac ctgcgggttc ggctcgcggt cgacgcggaa ctcgccggca tcggccccga
     3541 agacctcctc gtcgcggttg gccgcggcgt acaggagcag caccgggtcg cctgcagcga
     3601 tgggcacgcc gcgcagctcg gtgtcgaccg tggccgtccg catgaacgcg accaccggcg
     3661 tcgtccagcg cagccactcc tcgatgcacg acgggatcag ctcccgatcg gccacgaggc
     3721 gagccagctg gtcgggggtg ctcgccaggg cccagagacc gccggagatc atgttccggg
     3781 tggtctcgtt gcccgccacc agcagctggt tgaagaacat cagcagttcg tcgtcggtga
     3841 gcgtctcgcc gtcgatctcg acgttggcga gcaccgagat caggtcggtg ccatcggcgc
     3901 cccgccgatc ccgccccgcc gccatcaggt actcctgcat ctccgccatc agcgccatgc
     3961 gcgcttcgaa gccgaggtcg gaggcaccgg ggatggtggc atcggaccag aggacgaagc
     4021 ggtcgcggtc ctcgtcggcg atgccgagga ggtcggcgat gacgtggacc gggtagggct
     4081 cggcgagggc cggcacgatg tcgacgggct cgccggtggg cagctcgtcg aggagccggg
     4141 tcacgagggc ccggaggggc gcttcgaggg ctcggatccg gctgggcgag aagcccggct
     4201 ggaccagctt gcggtagcgg gtgtgcaccg gcggatcggt gtgcatcatc gtcggcggcg
     4261 aggggtactc gatgccgatc tccatggtca agatgccctt gccggactgg aagcggctcg
     4321 ggtcgcggga tacctcgagc acctccggct gcgtgctgac gacccaccac ccggcgccgt
     4381 cgtccggctg gtgccagacg atcggcgcct cctcgcgcag ccgggcgtag agcgggaacg
     4441 ggtcgccggc gtagaaggcc tcgtcggcga gcggtgccgt cagctccggc gccgtgggcc
     4501 ggttcacgct cacgctcgcg cttctcggaa cgtgacgcgg gtggcccacg agcgacggac
     4561 cggcgtgccg gtggcaccga ccgcaccgac ggctccgggg tcgatcggca tgcgcctact
     4621 cctcgtcgaa gcccagcatg ttggcgttcg ggcgggcttc ggccaccagc cgccgcacca
     4681 catcgccgag cgccgccggg
//
LOCUS       DATMFS010000630         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_116012, whole genome shotgun sequence.
ACCESSION   DATMFS010000630 DATMFS010000000
VERSION     DATMFS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116012"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VNQ33_12055"
     CDS             complement(<1..1260)
                     /locus_tag="VNQ33_12055"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWJ98886.1"
                     /translation="MSATDVTAEAAGEACRLEDLQISGMTCASCAARIERRLNRLDGV
                     EATVNYATEAARVSAPEHVSRQDLVAQVEAAGYGVVEPVPAAHPEHGVSPHEGHEGHH
                     VELGDADLRQRVVTSTVLTLPVLVMAMVPAAQFDHWQWISLALASPVVIWGGWPFHRA
                     AWTNLRHGAATMDTLISVGTLAAYGWSLYALVFTPAGDDGMTMAFDLVPRQGGGAHEI
                     YLETAAVVIVLILAGRWFEARAKRRSGAALRALLELGAKDVAVQRDGAEVRIPVDQLV
                     VGDRFVVRPGEKVATDGTVVEGRSALDTSLLTGEPVPVDVGPGDSVAGATVNTSGRLV
                     VEAARVGADTALARIAQLVADAQTGKAEVQRLADRVSAVFVPIVIAIAAATLGYWLGT
                     GAPAAEAFTAAVAVLIIACPCALGLATP"
     gene            complement(1257..1442)
                     /locus_tag="VNQ33_12060"
     CDS             complement(1257..1442)
                     /locus_tag="VNQ33_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98887.1"
                     /translation="MSGVHPAAKLAGFAVILVASFGAAFGAGRLVDGPDDRPARPTAT
                     TSTSVVATTTTDHGAHP"
     gene            complement(1439..1654)
                     /locus_tag="VNQ33_12065"
     CDS             complement(1439..1654)
                     /locus_tag="VNQ33_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HWJ98888.1"
                     /translation="MTTTATYAVTGMSCQHCVDAVTAEVGRIVGVEQVQVDLPTGAVT
                     VTSNAPLALDEVRSALDEAGYDLADEG"
     gene            1851..2954
                     /locus_tag="VNQ33_12070"
     CDS             1851..2954
                     /locus_tag="VNQ33_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWJ98889.1"
                     /translation="MRAAVLEEAGRPLAVVDDLVVDEPKAGEVAVTVKHCGVCHSDVS
                     IADGTFPSALPVVLGHEAAGVVAAVGPGVTTAAVGDHVVLTPCPPCGTCPWCIRGEWS
                     LCVSSDSMMTGAHPDGGTRLSRHGDVVYRGVGVAAFAETVIIQANGAVKIPDDVPLDV
                     ACVVGCAVQTGVGAVLNTAAVREGDTVLVMGAGGIGLSIIQGARVAGAGQITVSEPDP
                     ERRATALSMGATHGIDPTSDDLLAAVFDLSPHGIGADFAFDAVGRSALVAAGIDATRK
                     GGTTVMVGVPGLDDPFSYPIPAVLAIGEKKLIGSLLGSCNSLRDIPRLIALMQNGQLD
                     LASLITARRGLDDINRAFDDIRTTTGIRTVIDF"
BASE COUNT          413 a         1164 c         1111 g          410 t
ORIGIN      
        1 gggcgtggcc aggccgaggg cgcaggggca ggcgatgatc aacacggcga ccgccgcggt
       61 gaaggcctcg gcagccggtg cgccggtgcc gagccagtac ccgagggtgg cggcggcgat
      121 ggcgatgacg atcggcacga agaccgccga cacgcggtcg gccaggcgtt ggacctcggc
      181 cttgcccgtc tgggcgtcgg ccaccagctg ggcgatgcgg gcgagcgcgg tgtcggcgcc
      241 gacgcgagcg gcctcgacca cgagccggcc cgaggtgttg accgtggcgc cggcgacgct
      301 gtcgcccggc cccacgtcga ccgggacggg ctcgccggtc agcagcgacg tgtcgagcgc
      361 ggaccggccc tcgacgaccg tgccgtcggt ggcgaccttc tccccggggc ggaccacgaa
      421 gcggtcgccc accacgagct gatcgacggg gatgcggacc tcggcgccgt ctcgctggac
      481 ggcgacgtcc ttggccccca gctccagcag ggcgcggagg gccgccccag accggcgctt
      541 ggcgcgggcc tcgaaccagc ggccggcgag gatcagcacg atcacgaccg ccgcggtctc
      601 gaggtagatc tcgtgggcac cgccgccctg ccgaggcacc aggtcgaagg ccatcgtcat
      661 gccgtcgtcg ccggccggcg tgaacacgag ggcgtagagc gaccatccgt aggccgcgag
      721 cgtgccgacc gagatcagcg tgtccatggt ggcagcgccg tggcgcaggt tggtccacgc
      781 ggcgcggtgg aacggccagc cgccccagat caccacgggc gaggcgaggg cgagggagat
      841 ccactgccag tggtcgaact gggcggcggg gaccatggcc atcacgagca cggggagggt
      901 caggacggtc gaggtgacga cgcgctggcg gaggtcggca tcgccgagct ccacgtggtg
      961 gccctcgtgg ccctcgtgcg ggctgacgcc gtgctcgggg tgcgccgccg gaaccggttc
     1021 gacgaccccg tagccggcag cctcgacctg agccaccagg tcctggcgcg agacgtgctc
     1081 cggcgccgac acccgggcgg cctccgtggc gtagttcacg gtcgcctcga cgccgtcgag
     1141 cctgttgagc cgacgctcga tgcgggccgc gcacgatgcg caggtcatgc cgctgatctg
     1201 gaggtcctcc agccggcacg cctcgccagc cgcctcggcg gtgacgtcgg tggcgctcac
     1261 gggtgcgctc cgtggtccgt ggtcgtggtg gccacgaccg acgtcgacgt ggtggcggtc
     1321 gggcgtgccg gccggtcgtc gggaccgtcg accaggcggc cggcgccgaa cgcggcgccg
     1381 aagctggcca cgaggatcac ggcgaagccg gcgagcttgg cggccgggtg cacgccgctc
     1441 acccctcgtc ggcgaggtcg taccccgcct cgtcgagcgc gctgcggacc tcgtcgagcg
     1501 cgagcggcgc gttgctggtg acggtgacgg cgcccgtcgg caggtcgacc tggacctgct
     1561 cgacgccgac gatccgaccg acctcggcgg tgaccgcgtc gacgcagtgc tggcagctca
     1621 tcccggtgac ggcgtacgtg gcggtggtgg tcatgacggg ctcctccggg tggtcctgtg
     1681 tcagcaccct atacccctag ggggtatttt ccaccggtca tccgcctcac cacacgcctg
     1741 agacgatcga ccgccgggca ccgtgcgatc ccctcgccat ggatcccgcc gcccgccgcg
     1801 acgtggcagg gtggcgcccg caccacccac cgctcacatc aggggacgac atgcgcgctg
     1861 cagtgctgga agaggccggc cgaccgctgg cggtcgtcga cgacctcgtg gtcgacgagc
     1921 ccaaggccgg cgaggtcgcc gtcaccgtca agcactgcgg cgtctgccac agcgatgtga
     1981 gcatcgccga cgggaccttc ccctccgcgc tgccggtggt gctcggccac gaggccgccg
     2041 gcgtggtcgc cgcggtcggc cccggcgtca ccaccgccgc cgtcggcgat cacgtggtgc
     2101 tgacaccttg cccgccgtgc ggcacgtgcc cgtggtgcat ccgcggcgag tggagcctct
     2161 gcgtcagcag cgactcgatg atgaccggcg cccaccccga cggcgggacc cggctctccc
     2221 gccacggcga tgtcgtgtac cgcggcgtcg gcgtggccgc cttcgccgag acggtgatca
     2281 tccaggccaa cggtgccgtc aagatccccg acgacgtgcc cctcgacgtg gcctgcgtcg
     2341 tcggctgcgc cgtgcagacg ggcgtcggcg ccgtcctcaa caccgcggcg gtccgcgagg
     2401 gcgacaccgt gctggtgatg ggcgccggag gcatcggcct gtcgatcatc cagggcgcgc
     2461 gggtggccgg cgcgggccag atcaccgtga gcgagcccga ccccgaacgc cgcgccaccg
     2521 ccctctccat gggcgcgacc cacgggatcg acccgacgtc cgacgacctg ctggccgccg
     2581 tcttcgacct ctccccccac ggcatcggcg ccgacttcgc cttcgacgcc gtcggccgat
     2641 ccgcactcgt ggccgcgggc atcgacgcca cccgcaaggg cggcaccacc gtgatggtcg
     2701 gcgtgcccgg actcgatgac cccttctcct acccgatccc cgccgtgctg gcgatcggcg
     2761 agaagaagct gatcgggagc ctcctcggca gctgcaactc cctccgagac atcccccggc
     2821 tgatcgccct catgcagaac gggcagctcg acttggcctc gctcatcacc gcccgccggg
     2881 gcctcgacga catcaaccgg gccttcgacg acatccgcac caccaccggc atccgcaccg
     2941 tcatcgactt ctgatcgccg gcacagcccc gctcaggccg gtggttcacc ccgtgcaggc
     3001 acggtggccc ccgcctggtc tgtgacggcc ctcacctcgg tcgcggatct cgcgggtccg
     3061 tggcctcgtg cctcaaccgg agcgccccac cccccccc
//
LOCUS       DATMFS010000631         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_275342, whole genome shotgun sequence.
ACCESSION   DATMFS010000631 DATMFS010000000
VERSION     DATMFS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275342"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..750)
                     /locus_tag="VNQ33_12075"
     CDS             complement(52..750)
                     /locus_tag="VNQ33_12075"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWJ98890.1"
                     /translation="MAPRVKVHRASAQRRRAALLEAAAELAAELGAGAVTHRAVAARA
                     GVPLSTTSYFFSSIDELVTEALRLGATERVSDFDAAERAAVLSVDVPIGEVIDAVVDQ
                     ALAASRNAEGSQVEYYLAAGRQPDLQPEASATVQRFAERVARQLERTGAVRADEVGWA
                     ITALGDGAMLHRLAQVDTDHRQRLMSGLRVLVAAAMLDDDEIDELLSRYDTASGVVHD
                     PAAVADGQALPAPA"
     gene            complement(843..1631)
                     /locus_tag="VNQ33_12080"
     CDS             complement(843..1631)
                     /locus_tag="VNQ33_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ98891.1"
                     /translation="MILDSFLLTDQVAIVTGAGRGIGAACALALAEAGADVVISARSE
                     DQLAEVAAQIEATGRRAHIVVADLSDLDAVAGLATAAKGAFGRLDVVVDNLGGTMPRP
                     FEHTSPRFLAEAFSFNVVTAHALNRAALPVMLETGGGSIVGISSIMGRVPGRGYLAYG
                     TAKAGLAHYTRLAARDLAPKVRVNAIAVGSIATSALDIVMGDDAMRTQMEEATPLRVI
                     GQPTHIAATVVYLASPAGGYVTGKVVEVDGGLEQPNLDLGLPDL"
     gene            1767..>2817
                     /locus_tag="VNQ33_12085"
     CDS             1767..>2817
                     /locus_tag="VNQ33_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ98892.1"
                     /translation="MGDLVSALEGIVGAANVATGGAIAEDLTHDEAITALAVTPAAVA
                     RPATTEEVAAIVALAAEQGVPLTARGSGTGLSGACIPQPDGILVSFDRMAEILEIDLD
                     NHVARVQPGVTLAQLDEATAAHGLVYPVFPGENSASLGGNVATNAGGMRAVRYGVTRH
                     QVLGLTAVLGTGETITTGGKFVKATSGYDLTQLIIGSEGTLAIATEATLRLHPRLTHG
                     ATVLIPFPGLDEVAASIPRIVGSGLMPTILEYIDFITMSAITTAQDLELGVPQEIKDA
                     AMAYLVVVLENRNDERVEEDVHELAELATELGALDLYVLPTGAGAALIDAREKAFWTA
                     KANGANDIIDMVVPRG"
BASE COUNT          398 a         1032 c         1024 g          363 t
ORIGIN      
        1 gggcgcgacc cgaacgcggg aggcggcccg aacgcgacga gaccaggcgg gtcaggcggg
       61 agcgggtagg gcctgcccgt cggccacggc cgccggatcg tggacgaccc cggaggccgt
      121 gtcgtagcga gacagcagct cgtcgatctc gtcgtcgtcg agcatggcgg cggccacgag
      181 cacgcggagg ccgctcatga ggcgctggcg gtgatcggtg tcgacctggg caaggcggtg
      241 caacatggcg ccgtcgccca gcgcggtgat ggcccacccg acctcgtcgg cgcggaccgc
      301 gccggtgcgc tcgagctggc gggcgacgcg ctcggcgaac cgctgcaccg tggccgacgc
      361 ctccggctgc aggtccggct ggcggcccgc cgcgaggtag tactcgacct ggctgccctc
      421 ggcgttgcgc gaggcagcca gcgcctggtc gaccacggca tcgatgacct cgccgatggg
      481 tacgtcgacc gacaagacgg cggcgcgctc ggcggcgtcg aagtccgaga cccgttcggt
      541 ggccccgagc cgcagcgcct cggtgacgag ctcgtcgatg gagctgaaga agtaggaggt
      601 ggtcgacagc gggacgcccg cacgggcggc gaccgcccgg tgcgtgacgg cgccggcgcc
      661 cagttcggcg gcgagctcgg cggccgcctc gagcagcgcg gcgcggcgcc gctgcgcgct
      721 cgctcgatgg accttcactc gtggtgccac atcgctcact gtaggtcact ccaacgccgt
      781 gtttccggcg gtcacatacc cgtgcgggtg atgcctgcct ccccctcagc cccacccgca
      841 ggtcacaggt cgggaaggcc cagatccagg ttcggctgct cgagcccgcc gtcgacctcg
      901 acgaccttgc ccgtcacgta gccgccggcc ggcgaggcga ggtagacgac ggtggcggcg
      961 atgtgggtgg gctggccgat cacccgcagc ggcgtggcct cttccatctg ggtccgcatg
     1021 gcgtcgtcgc ccatgacgat gtcgagggcc gacgtggcga tggagcccac ggcgatggcg
     1081 ttcacccgca ccttcggcgc caggtcgcgc gccgccaggc gcgtgtagtg cgccagcccg
     1141 gccttcgccg tcccgtaggc gaggtagccg cggccgggga cgcggcccat gatcgaggag
     1201 atcccgacga tcgagccgcc gccggtctcc aacatgacgg ggagcgcggc gcggttgagg
     1261 gcgtgggcgg tgacgacgtt gaacgagaac gcctccgcca ggaaccgcgg cgaggtgtgc
     1321 tcgaacgggc ggggcatggt gccgcccagg ttgtcgacca cgacgtcgag tcggccgaag
     1381 gcgcccttcg ccgcggtggc gagcccggcg acggcgtcga ggtcgctgag gtcggccacc
     1441 acgatgtgcg cccgccggcc ggtcgcctcg atctgggcgg cgacctcggc gagctggtct
     1501 tccgaccgcg ccgagatcac gacgtcggcg ccggcctcgg ccaaggcgag cgcgcacgcc
     1561 gcgccgatcc cgcgcccggc gccggtgacg atggcgacct gatcggtgag gaggaacgag
     1621 tcgaggatca agaggagctc ccggtgccgg acctgacggc gcgtcaggct aggtcgggcg
     1681 gtccgtccgg ggggcgtggc atgctggcgc cctcgttcgc cggacgcaga cctcgggtct
     1741 cgcggcgcca gcatgcaggg ggcttcatgg gtgatctcgt cagcgcactg gagggcatcg
     1801 tcggtgccgc caacgtggcc accggaggcg ccatcgccga ggacctgacc cacgacgagg
     1861 ccatcaccgc gctggccgtg acccccgcgg ccgtcgcccg gccggccacc accgaggagg
     1921 tggccgccat cgtcgccctg gccgcggagc agggcgtgcc gctcaccgct cgcggctcgg
     1981 gcaccggcct ctccggcgcc tgcatcccgc agcccgacgg catcttggtc tcgttcgacc
     2041 ggatggccga gatcctcgag atcgacctgg acaaccacgt ggcccgcgtc cagcccggcg
     2101 tcaccctcgc ccagctcgac gaggccaccg ccgcccacgg cctcgtctac ccggtcttcc
     2161 ccggcgagaa ctcggcgagc ctcggcggca acgtcgccac caacgccggc ggcatgcgcg
     2221 ccgtccgcta cggcgtgacc cgccaccagg tgctgggcct caccgcggtc ctcgggaccg
     2281 gcgagaccat caccaccggc ggcaagttcg tgaaggccac gtccggctac gacctcacgc
     2341 agctcatcat cggctccgag ggcacgctgg ccatcgccac cgaggccacc ctccgcctgc
     2401 acccgcgcct cacgcacggg gccacggtcc tgatcccgtt ccccgggctc gacgaggtag
     2461 ccgcctcgat cccccgcatc gtcggctcgg gcctgatgcc gacgatcctc gagtacatcg
     2521 acttcatcac gatgagcgcc atcacgacgg cgcaggacct cgagctcggg gtgccgcagg
     2581 agatcaagga cgcggcgatg gcgtacctcg tcgtggtcct ggagaaccgc aacgacgagc
     2641 gggtcgaaga ggacgtgcac gagctggccg agctggccac cgagctcggt gccctggacc
     2701 tctacgtgct gcccaccggc gccggcgccg ccctcatcga cgcccgggag aaggcgttct
     2761 ggacggcgaa ggccaacggc gccaacgaca tcatcgacat ggtggtgccc cgcggct
//
LOCUS       DATMFS010000632         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_161507, whole genome shotgun sequence.
ACCESSION   DATMFS010000632 DATMFS010000000
VERSION     DATMFS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161507"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VNQ33_12090"
     CDS             <1..456
                     /locus_tag="VNQ33_12090"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWJ98893.1"
                     /translation="QIEFSRTIQNIAEDTGTEITPGAMWDAFSSTYLAEDAPYVLRST
                     ELRTDAEGVTSITAQLVVDGEPATVTGSGNGPIAAFVAAIGTAGIEIDVVDYHEHSMG
                     AGAGATAVAYVETVDPASTVRWGIGTDPNIITASLKAVLGAASRARSEA"
     gene            complement(601..1149)
                     /locus_tag="VNQ33_12095"
     CDS             complement(601..1149)
                     /locus_tag="VNQ33_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98894.1"
                     /translation="MDRTRRLLALTAVGAFLLVGTAACGSSGGSDRSSDDSAKTTVAD
                     GASDTGTGDTAADDGSSGGGDEQDCDATADSNNLSGDSVVLFSADQDELSESDLADDA
                     IATVAADRSSMDPDTIEIGAGEMFGFEAEDGGSLDAVVIGCAGGQTMVPGTAIGFVIT
                     EPGTYPVSLDVAGTDLGTVVVS"
     gene            1313..2545
                     /locus_tag="VNQ33_12100"
     CDS             1313..2545
                     /locus_tag="VNQ33_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ98895.1"
                     /translation="MTIDFTLTPELDELRLRIRTFVDDVIKPTEAELTDPDHIEDRAA
                     YLTKLVGMRTKAQEAGIWLPHMPAEWGGMGLGHVELAMVQAEAAKAYYGPFVFNCMAP
                     DEGNMHTLLNWATDEQKQTYLKPLCEGTTMSCFAMTEPEVAGSDPTLIRTHAVQDGDE
                     WVINGHKWFISNARRASFAILICRTEDNPDLPQAANTAFIVETHWEGWNRVREVETMH
                     GGTGHSEIVIEDLRVPDANMLGGRGQGHLLGQSRLGPARLAHCMRWIAQAETALDMMV
                     DRSLNRFSHGSMLAEKQGVQWMIADSTMELYQAKLMVLHAAYRIDNKLDFKSEVSMAK
                     HFVANMLGRVIDRSIQVHGALGYSTDTPLAHMYQHARWARFADGADEIHQMRIAQRTI
                     AAYRDTGSTRTATGDLPI"
     gene            complement(2635..>3375)
                     /locus_tag="VNQ33_12105"
     CDS             complement(2635..>3375)
                     /locus_tag="VNQ33_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98896.1"
                     /translation="RPGPSQAVRYIRGIFPMILQRNPNTATTDYWAARWKAATMAYPR
                     KLEQVPLGIINSNEYRRLRVRESYQRILGRAVDPSALTYWSTKAARGWSYERIDTTLL
                     SSAEFLRNGDLETHVRRTYVAVLGRQPSQSELNRWKATLGPTGTKGWSRFPLALQRST
                     EGFDVIIKQRYQVSTGQAPSSFARYIWQVGLRNGQSPERLWAQLLVSYDVLKNYPYTD
                     DDYEEGYEYDVPRASVVAAVHAAVDAVP"
BASE COUNT          536 a         1210 c         1155 g          474 t
ORIGIN      
        1 cagatcgagt tctcccggac gatccagaac atcgccgagg acacgggcac cgagatcacg
       61 cccggcgcca tgtgggatgc gttctcctcg acctacctcg cagaggatgc cccctacgtg
      121 ctccgctcca ccgagctgcg caccgatgcc gagggcgtca cgtcgatcac cgcccagctg
      181 gtggtcgacg gggagccggc cacggtgacc ggctcgggca acggcccgat cgccgcgttc
      241 gtcgccgcca tcggcaccgc cggcatcgag atcgacgtgg tcgactacca cgagcactca
      301 atgggcgccg gcgccggcgc cacggcggtc gcctacgtgg agacggtcga cccggccagc
      361 accgtgcgct ggggcatcgg caccgatccc aacatcatca cggcctcgct caaggccgtc
      421 ctcggcgccg cgtcccgcgc tcgctccgaa gcctgaggtc ccgacgcagc ggcaccccgt
      481 ggcggccggc ctggcacagg ccggtccgcc acgagggacg cgggtccgaa gcgaggggac
      541 cacggacgag gggcctgacc ggcgagggtc ccgtctgccc ccgccgatca gacgaacggg
      601 tcagctgacg acgacggtgc cgaggtcggt gccggcgacg tcgagcgaca ccgggtaggt
      661 ccccggctcg gtgatcacga acccgatggc ggtgcccggc accatggtct ggccgccggc
      721 acagccgatg accacggcat cgaggctgcc gccgtcttcg gcctcgaacc cgaacatctc
      781 accggcgccg atctcgatcg tgtcggggtc catcgaggag cggtcggcgg cgacggtggc
      841 gatggcgtcg tcggccaggt cggactcgga gagctcgtct tgatcagccg agaagagcac
      901 gaccgaatcg cccgagaggt tgttgctgtc cgccgtggcg tcgcagtcct gctcgtcacc
      961 gccgcccgag gatccgtcgt cagcggcggt gtcgccggtg ccggtgtcgc tcgcgccgtc
     1021 ggccaccgtg gtcttggcgc tgtcgtccga cgagcggtcg gaaccgccgc tcgatccgca
     1081 ggcggccgtg cccaccagga ggaaggcacc gacggcggtg agggcgagca ggcgacgggt
     1141 gcggtccacg gtggtgcgct cctggggaga ggtgacgggc tgagatcgtc ccactcctcg
     1201 tcggcgccgc ccaccgtcga gcggtcccaa accggtccca acctcgctca ggacccgcga
     1261 cttgacccgc gggtcaagac aggccgccgg atcggcggta ggttcccgat ccatgacgat
     1321 cgacttcacc ctcacccccg agctcgacga gctgcgcctg cggatccgga ccttcgtcga
     1381 cgatgtcatc aagcccaccg aggcggagct gaccgacccc gatcacatcg aggaccgcgc
     1441 cgcgtacctc accaagctcg tcggcatgcg caccaaggcg caagaagccg gcatctggct
     1501 gccgcacatg ccggcggagt ggggcggcat ggggctcggc cacgtggagc tggcgatggt
     1561 gcaggccgaa gcggccaagg cgtactacgg gccgttcgtc ttcaactgca tggcccccga
     1621 cgaggggaac atgcacaccc tcctcaactg ggcgaccgac gagcagaagc agacgtacct
     1681 caagccgctg tgcgagggca cgacgatgag ctgcttcgcc atgaccgagc ccgaggtcgc
     1741 cggctcagac ccgaccctca tccgcaccca cgccgtgcag gacggcgacg agtgggtcat
     1801 caacggccac aagtggttca tctccaacgc ccggcgggcc agcttcgcca tcctcatctg
     1861 ccggaccgag gacaaccccg acctgcccca ggcggccaac accgccttca tcgtggagac
     1921 ccactgggag ggctggaacc gcgtgcgcga ggtggagacg atgcacggcg gcaccgggca
     1981 ctccgagatc gtcatcgagg acctgcgcgt gcccgacgcc aacatgctcg gcggccgcgg
     2041 ccagggccac ctgctcggcc agtcccgcct cgggcccgcc cgcctcgccc actgcatgcg
     2101 ctggatcgcc caggccgaga ccgccctcga catgatggtc gaccggtcgc tcaaccgctt
     2161 cagccacggg tcgatgctgg ccgagaagca gggcgtccag tggatgatcg ccgactccac
     2221 catggagctg taccaggcga agctgatggt cctccacgcc gcctaccgca tcgacaacaa
     2281 gctcgacttc aagtccgagg tgtcgatggc gaagcacttc gtggccaaca tgctgggccg
     2341 ggtgatcgac cggtcgatcc aggtgcacgg cgcgctcggc tactccaccg acaccccgct
     2401 ggcccacatg taccagcacg cccgctgggc ccgcttcgcc gacggggccg acgagatcca
     2461 ccagatgcgc atcgcccagc gcaccatcgc cgcctaccgc gacaccggca gcacccgcac
     2521 cgccaccggc gacctgccga tctgacggcg tccagccccg cccccccggc gttcgacgat
     2581 ccccgcccgc tgacctctcg ggtacggcgg gcggggtcga tcgtcggcac gaggtcaagg
     2641 cacggcgtcg accgcggcgt ggacggcggc cacgacgctg gcgcgcggga cgtcgtactc
     2701 gtacccctcc tcgtagtcat cgtccgtgta ggggtagttc ttgaggacgt cgtagctcac
     2761 gagcagctgg gcccacaggc gctccggcga ctggccgttg cgcaggccga cctgccagat
     2821 gtagcgggcg aacgacgacg gcgcctggcc ggtggacacc tggtaacgct gcttgatgat
     2881 gacgtcgaag ccctcggtgc ttcgctgcag cgccagcggg aaccgcgacc agcccttggt
     2941 accggtgggg ccgagcgtgg ccttccatcg gttcagctcg gactggctgg gctggcggcc
     3001 gagcacggcg acgtaggtcc tgcggacgtg cgtctcgagg tctccgttgc ggaggaactc
     3061 cgccgaggag agcagcgtgg tgtcgatgcg ctcgtaggac cagccacgtg cggccttggt
     3121 gctccagtag gtcagcgccg acggatcgac cgcccggccg aggatgcgct ggtaggactc
     3181 ccggacccgc aagcggcggt actcgttgct gttgatgatg cccaggggca cctgctcgag
     3241 cttgcgcggg taggccatgg tggccgcctt ccacctggct gcccagtagt cggtggtggc
     3301 cgtgttcggg ttccgctgga ggatcatcgg gaagatgccc cggatgtagc ggacggcctg
     3361 cgacgggccg ggccg
//
LOCUS       DATMFS010000633         5546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21858, whole genome shotgun sequence.
ACCESSION   DATMFS010000633 DATMFS010000000
VERSION     DATMFS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21858"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2596
                     /gene="valS"
                     /locus_tag="VNQ33_12110"
     CDS             <1..2596
                     /gene="valS"
                     /locus_tag="VNQ33_12110"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWJ98897.1"
                     /translation="NVPEKPSLEGLEAKWGAVWDEQGTYTFDRTKERAEVFSIDTPPP
                     TVSGSLHVGHVFSYTHTDLVARYQRMAGKEVFYPMGWDDNGLPTERRVENFYGVRCDP
                     TVPYVDGYTPPEKPAKKRQDYDAISRRNFVELCEELTAEDEKVFEGLYRHLGLSIDWS
                     TTYSTVSLKSQRVSQRAFLRNLKRGEAYSSEAPSLWDTTFQTAVAQAELEDRERPGAY
                     HDIAFGRPDGGEPIVISTTRPELLVACVALVAHPDDERYQDLFGTTVTTPVFGVEVPV
                     KAHRLAEPDKGTGIAMICTFGDLTDVIWWRELQLETRAVMRKDGRLSLDTPDWLTTPE
                     ARAAYERFAGKAPGGAQIVMVELLTESGDLLGEPKKIVHPVKFYEKGDKPLEIVTTRQ
                     WYIPNGGRSADLREALIGRGRELVWHPDYMRHRYEDWVNGLNGDWLISRQRFFGVPIP
                     LWYRLDADGEPQYDDPIIPAEDTLPVDPQSHIPPGFTSDQRNQPGGFAADPDIMDTWA
                     TSSLTPQIATGWEEDPALFASTFPMDMRPQAHEIIRTWLFSTVVRSHYEHGEVPWANA
                     ALSGWILDPDRKKMSKSVGNVVVPTDLLEQHGADAVRYWAANGRPGTDTAFDPGQMKI
                     GRRLAIKVLNASKFALGAGDAPVDAPVTEPIDLAMLAELAQLVDDATTSFADFDYARS
                     LQRTEDFFWRFCDDYLELVKARAYREDDPAALSARVALRTAVSTLLRLFAPILPYVTE
                     EVWSWFAEGSIHRAAWPTSGELRAVVGDAPGADGVPSPLVVAADVLSRIRKAKSDAKV
                     SQRAEVQLVTVTDTSDRLVALAAAEGDVKAAGSVVELATAELAGDGEGSVDVALAPVE
                     PASTADPT"
     gene            2593..2847
                     /locus_tag="VNQ33_12115"
     CDS             2593..2847
                     /locus_tag="VNQ33_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HWJ98898.1"
                     /translation="MKPGPSSPTRDGWLDQPHPSRLDPGHPRRDEILARHARAVAAGL
                     STYLDPATGYTVLTAQYLSDRGYCCSQGCRHCPWEGAGPG"
     gene            3057..4556
                     /locus_tag="VNQ33_12120"
     CDS             3057..4556
                     /locus_tag="VNQ33_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWJ98899.1"
                     /translation="MDVVSLSRWQFGITTVYHFWMVPLTIGLGLTVASFQTRWYRSGD
                     EAYLRLTRFFGKLFLINFAMGVVTGIVQEFQFGMNWANYSRFVGDVFGAPLAMEALVA
                     FFLESTFLGLWIFGWERLSPRLHLATIWAAAIGATLSAYFIVAANSWMQHPVGAVYNA
                     ETGRAEMTDIGAVLTNSTTLAAYPHVIAGALLVAAVFVGGISAWLLMRDRPMPAGDRV
                     ALHKAVRTSIYLTFAMGIAVAVSGDYQAKLMFEQQPMKMAAAEALCDTEQPAGLSVFA
                     VGDVSAECDVRTIVIPHLLSFMATGSFDGKVEGVKDINAQYREQFGPGDYRPNLVITY
                     WSFRAMIGFGALASLGAVVAWWKTRNGKELPTGRRWLRVGSIAVIATPFLANSFGWIF
                     TEMGRQPWVVAPNPEGIPELRLMTADASSPAVSAAMVWTTLIGFTLLYGALLVVELFL
                     LKRYVQAGPEAVMPFQPPDPPGPDDGDGSDDGAAGPVRDEEADVLAFAY"
     gene            4568..>5546
                     /gene="cydB"
                     /locus_tag="VNQ33_12125"
     CDS             4568..>5546
                     /gene="cydB"
                     /locus_tag="VNQ33_12125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWJ98900.1"
                     /translation="MELSTFWFILIGVLWTGYFFLEGFDFGVGMLLHPFAHDDTERRV
                     LINTIGPVWDGNEVWLITAGGATFAAFPNWYATLFSGFYLALFLILVSLILRGVAIEY
                     RGKVDSEVWRRRWDWAIAFGSWVPSVLWGVAFANIVAGVPINRNGDFTGTLLTLVNPF
                     ALLGGVVTASLFLLHGLHFVALKTEGEIHDRATRTSLRVAPVTVVAGAAFLVWNQVER
                     GETWTWVPVLVAAVSLVASAAATRGGRDGWAFAATGIVTAAAVVALFGSLYPDVMPSS
                     TDPAFSLTVESASSTPYTLKIMSWVAVVMTPVVLGYQAWSYWVFRKRIGT"
BASE COUNT          794 a         2128 c         1782 g          842 t
ORIGIN      
        1 gaacgttcct gagaagccgt ccctcgaggg cctcgaggcg aagtggggcg cggtgtggga
       61 cgagcagggc acgtacacgt tcgaccggac gaaggagcgg gccgaggtct tctccatcga
      121 cacgcccccg cccaccgtca gcggctcgct gcacgtcggc cacgtcttca gctacaccca
      181 caccgacctc gtcgcccggt accagcgcat ggccggcaag gaggtcttct acccgatggg
      241 gtgggacgac aacgggctgc ccaccgagcg ccgggtcgag aacttctacg gcgtccgctg
      301 cgacccgacg gtcccctacg tcgacggcta caccccgccg gagaagccag ccaagaagcg
      361 ccaggactac gacgccatca gccgccgcaa cttcgtcgag ctgtgcgaag agctcaccgc
      421 ggaggacgag aaggtcttcg agggcctgta ccgccacctc ggcctgtcga tcgactggtc
      481 caccacgtac tccacggtct ccctgaagag ccagcgggtc agccagcggg cgttcctccg
      541 gaacctgaag cggggcgagg cctactcgtc ggaggcgccg agcctgtggg acaccacgtt
      601 ccagaccgcc gtcgcccagg ccgagctcga ggaccgcgag cgtccgggcg cctaccacga
      661 catcgccttc ggtcgccccg acggcggcga gccgatcgtc atctccacca cccggcccga
      721 gctgctcgtc gcgtgcgtcg ccctcgtcgc ccaccccgac gacgagcgct accaggacct
      781 cttcggcacc acggtcacca cgcccgtctt cggcgtcgag gtcccggtca aggcgcaccg
      841 cctggcggag cccgacaagg gcaccggcat cgccatgatc tgcaccttcg gcgacctcac
      901 cgacgtcatc tggtggcggg agctgcagct ggagacgcgg gccgtcatgc gcaaggacgg
      961 gcgcctgtcg ctcgacaccc cggattggct gaccacgccg gaggcccgcg ccgcgtacga
     1021 gcgcttcgcc ggcaaggcgc ccggcggcgc ccagatcgtg atggtcgagc tgctcaccga
     1081 gagcggcgac ctcctcggcg agccgaagaa gatcgtccac ccggtcaagt tctacgagaa
     1141 gggcgacaag cccctcgaga tcgtcacgac ccgccagtgg tacatcccca acggcggccg
     1201 ctcggccgac ctgcgcgagg ccctcatcgg gcggggccgc gagctggtct ggcaccccga
     1261 ctacatgcgc caccgctacg aggactgggt caacggcctc aacggcgact ggctcatctc
     1321 ccgccagcgg ttcttcggcg tcccgatccc gctctggtac cgcctcgacg ccgacggcga
     1381 gccgcagtac gacgacccga tcatccccgc cgaggacacg ctgccggtcg acccgcagtc
     1441 gcacatcccg ccggggttca cctccgacca gcgcaaccag ccgggcggct tcgcggccga
     1501 ccccgacatc atggacacct gggccacctc gtcgctcacc ccgcagatcg ccaccggctg
     1561 ggaggaggac ccggcgctgt tcgcgtcgac cttcccgatg gacatgcggc cgcaggccca
     1621 cgagatcatc cgcacctggc tgttctcgac ggtcgtccgc agccactacg agcacggcga
     1681 ggtcccctgg gccaacgccg ccctgtcggg gtggatcctc gacccggacc gcaagaagat
     1741 gtcgaagtcg gtcggcaacg tcgtcgtccc caccgacctg ctcgagcagc acggcgccga
     1801 cgccgtccgc tactgggcgg ccaacggccg tcccggcacc gacaccgcgt tcgacccagg
     1861 ccagatgaag atcggccgcc gcctcgccat caaggtcctc aacgcctcga agttcgctct
     1921 gggcgccggc gatgcacccg tcgacgcgcc ggtcaccgag cccatcgacc tggcgatgct
     1981 ggccgagctg gcccagctcg tcgacgacgc caccacctcg ttcgccgact tcgactacgc
     2041 ccgctcgctc cagcgcaccg aggacttctt ctggcgcttc tgcgacgact acctcgagct
     2101 ggtcaaggcc cgggcgtacc gggaggacga ccccgccgcg ctgtcggccc gggtcgccct
     2161 gcgcaccgcg gtctcgacgc tgctgcggct gttcgccccg atcctcccct acgtcaccga
     2221 ggaggtctgg tcgtggttcg ccgagggctc gatccaccgc gccgcgtggc cgacatcggg
     2281 cgaactgcgg gccgtcgtcg gcgacgcccc cggtgccgac ggcgtcccgt cgccgctcgt
     2341 cgtcgccgcc gacgtcctca gccgcatccg caaggccaag tccgacgcca aggtctccca
     2401 gcgcgccgag gtccagctgg tcacggtcac cgacacgtcg gaccgcctcg tcgcgctggc
     2461 cgcggccgag ggcgacgtca aggccgccgg ctcggtcgtc gagctcgcca cggccgagct
     2521 ggcgggcgac ggcgaaggct ccgtcgacgt cgccttggca ccggtcgagc ccgcctcgac
     2581 ggccgacccg acgtgaagcc gggcccctcg agccccacgc gcgacggctg gctcgaccag
     2641 ccccatccga gccgcctcga cccgggccac ccgcgccgcg acgagatcct cgctcgccac
     2701 gcccgggccg tggccgccgg cctgtcgacc tacctcgatc cggccaccgg ctacacggtc
     2761 ctcaccgccc agtacctcag cgaccgcggc tactgctgca gccagggctg ccgccactgc
     2821 ccctgggagg gcgctggccc cgggtgacca cccggcccga gccagcccga ccgagccgaa
     2881 cgttcggtgc gaacggttgg cgtgcagcca cgggcatcga acggttcgtg ggtgccaggg
     2941 ccgcgagcca tcatcgccag aaacgatgat gatagagccg cccatctcga caggcgatcc
     3001 accggctagg gtcagcggcg agctcccccg cccgcccccc atgcgaagga accgcaatgg
     3061 acgtggtcag cctctcccga tggcagttcg ggatcaccac cgtgtaccac ttctggatgg
     3121 tccccctgac catcgggttg gggctcacgg tggcctcctt ccagacccgc tggtaccgct
     3181 cgggcgacga ggcgtacctg cgcctcacca ggttcttcgg gaagctgttc ctcatcaact
     3241 tcgccatggg cgtggtcacc ggcatcgtcc aggagttcca gttcggcatg aactgggcca
     3301 actactcccg gttcgtgggc gacgtgttcg gcgcgccgct ggcgatggag gcgctggtcg
     3361 ccttcttcct cgagtccacg ttcctcggcc tctggatctt cggctgggag cgcctctcgc
     3421 cccggctgca cctggccacg atctgggcgg cggccatcgg ggccacgctg tcggcgtact
     3481 tcatcgtcgc cgccaactcc tggatgcagc accccgtcgg ggccgtctac aacgccgaga
     3541 ccggccgggc cgagatgacc gacatcgggg ccgtgctcac caactcgacc acgctcgccg
     3601 cctacccgca cgtgatcgcc ggcgccctgc tcgtggccgc cgtcttcgtg ggcggcatct
     3661 cggcctggct gctgatgcgg gaccggccga tgccggccgg cgaccgggtg gcgctgcaca
     3721 aggcggtgcg cacgtcgatc tacctgacct tcgccatggg catcgccgtg gcggtgagcg
     3781 gcgactacca ggccaagctc atgttcgagc agcagccgat gaagatggcc gccgccgagg
     3841 ccctgtgcga caccgagcag cccgccggtc tgtccgtgtt cgccgtcggc gacgtgagcg
     3901 ccgagtgcga cgtgcggacc atcgtcatcc cccacctcct ctcgttcatg gccacgggtt
     3961 cgttcgacgg gaaggtcgag ggcgtcaagg acatcaacgc ccagtaccgc gagcagttcg
     4021 gccccggcga ctaccgcccc aacctcgtca tcacctactg gagcttccgg gcgatgatcg
     4081 gcttcggggc cctcgccagc ctcggcgccg tcgtcgcctg gtggaagacc cgcaacggca
     4141 aggagctgcc caccggcagg cgctggctcc gcgtcgggtc catcgccgtc atcgccacgc
     4201 cgttcctggc caactcgttc ggctggatct tcaccgagat gggacgccag ccgtgggtgg
     4261 tggcacccaa cccggagggc atccccgagc tgcggctgat gacggccgac gcgtcgtcgc
     4321 ccgccgtctc ggccgcgatg gtgtggacga cgctcatcgg cttcaccctg ctgtacggcg
     4381 cgctcctggt ggtcgagctg ttcctgctca agcggtacgt gcaggccggc cccgaagccg
     4441 tgatgccgtt ccagccgccc gacccgcccg gccccgatga cggggacggt tccgacgacg
     4501 gcgctgccgg ccccgtccgc gacgaggagg ccgacgtgtt ggccttcgcc tactgatcgg
     4561 ggtcgtgatg gagctctcca ccttctggtt catcctcatc ggcgtcctct ggaccggcta
     4621 cttcttcctc gaggggttcg acttcggggt cgggatgctg ctacacccct tcgcccacga
     4681 cgacaccgag cggcgcgtcc tcatcaacac gatcggcccg gtctgggacg gcaacgaggt
     4741 gtggctgatc acggccggcg gcgctacgtt cgccgccttc ccgaactggt acgccacctt
     4801 gttctccggc ttctacctcg ccctgttcct gatcctcgtg tccctgatcc tgcgcggcgt
     4861 ggcgatcgag taccgaggca aggtcgacag cgaggtctgg cggcgccgct gggactgggc
     4921 catcgcgttc gggtcgtggg tgccgtcggt gctgtggggc gtggccttcg ccaacatcgt
     4981 ggccggtgtc ccgatcaacc ggaacggcga cttcacgggc acgctgctca cgctcgtcaa
     5041 cccgttcgcc ctgctcggcg gtgtggtcac cgcatccctg ttcctgctgc acggcctcca
     5101 cttcgtcgcc ctcaagaccg agggcgagat ccacgaccgg gccacgcgca cctcgctgcg
     5161 ggtcgccccg gtcaccgtcg tcgccggcgc cgccttcctc gtctggaacc aggtggagcg
     5221 cggcgagacc tggacctggg tcccggtgct ggtcgccgcc gtcagcctcg tcgcctccgc
     5281 agccgccacc cgcggcgggc gcgacggctg ggcgttcgcc gccaccggca tcgtcaccgc
     5341 cgccgccgtg gtcgccctgt tcgggtcgct ctaccccgac gtcatgccca gctccaccga
     5401 cccggcgttc agcctcaccg tcgagagcgc cagctccacc ccgtacacgc tgaagatcat
     5461 gagctgggtc gccgtcgtga tgacgcccgt cgtcctcggc taccaggcct ggtcctactg
     5521 ggtcttccgc aagcgcatcg gcaccc
//
LOCUS       DATMFS010000634         5703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_252431, whole genome shotgun sequence.
ACCESSION   DATMFS010000634 DATMFS010000000
VERSION     DATMFS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252431"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VNQ33_12130"
     CDS             <1..227
                     /locus_tag="VNQ33_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98901.1"
                     /translation="AVPGEALTSGSVDVDGRLLGVDNPVAFEASASQPPYGLVVMALL
                     LVGTVIALAVRRRRRRGRAGSVADTGFVAP"
     gene            complement(355..1320)
                     /locus_tag="VNQ33_12135"
     CDS             complement(355..1320)
                     /locus_tag="VNQ33_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ98902.1"
                     /translation="MTLISPNRLWLLVAVAGLVAAYVVAQHRRTAPAVRHPDLALIDS
                     FAPRWGGWRRHLTAFALVLALTAMVVGLARPAHAVTVERKEAVVMLTLDVSKSMTATD
                     VAPTRLAAAQKAAKEFVESAPEGYRIGLVTFDAQTHTLASPTTDRAELLSAIDGLEAA
                     NGTAAGDGLKTSVDVLKADAAAAAAEGATADDDTYRAIILLADGDSSTGTTLDQAADG
                     AVDEKIPVFTIAYGTADATIVVDGKTMAAGADPAAIAAVADKTGGTDYTASTSEELSS
                     VYGQIGTTIGTATEHQELTVPLSLLAAALLGLAIVATTAWTPRLT"
     gene            complement(1350..2309)
                     /locus_tag="VNQ33_12140"
     CDS             complement(1350..2309)
                     /locus_tag="VNQ33_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ98903.1"
                     /translation="MSFLHPERLLLLGAVVLLAAGYAAVWHRRTGAIARYAQPHLHDK
                     IAPERARGVRRHVAPALALGALSLVLVSAAQPTREQRVPRERGVVVLAIDTSLSMQAT
                     DISPTRLEAAIDGATDFVEELPEGIEVGLVSFDSNARLLVSPTTDHQSVVAAISTLTT
                     NARTATGEGVFASLDAVKTALDEDTAKGDDPAPAAIVLLSDGVETVGRSVDSATRAAV
                     DAEVPVSTIAYGTPTGTVTIEGQTMAVPADARTMEAVADATGGTSFQATSAGELKDVY
                     ADIQTTIGYETEPREVGRALLGGAFVVLLAAGGIAVATSTRPL"
     gene            complement(2306..3199)
                     /locus_tag="VNQ33_12145"
     CDS             complement(2306..3199)
                     /locus_tag="VNQ33_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWJ98904.1"
                     /translation="MTATAALASSERSLRLLELTINRKLDGLIHGDHQSIALGPGSEG
                     AEGRRYQPGDDVRRIDWNLTARSGDVHVRDTIAERELETWLVIDGSASLDYGTANQEK
                     RDLAASVVGAFGFLVVRAGNRIGAVVADGAETWVVPPRAGRSAVLDLVVRLQRRGRTT
                     GEGATLAAALRKTLLLARKRGLVIVVSDLLDGSDWPTELRSLAARHDVIVAHVTDPRE
                     DEIPNVGMLALVDPETGQRREVQTASKRFRERYAAAAAERRSTIDERLRRSRAQHLRV
                     STDRDWLRDVVRHIAARRRRR"
     gene            complement(3196..4218)
                     /locus_tag="VNQ33_12150"
     CDS             complement(3196..4218)
                     /locus_tag="VNQ33_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ98905.1"
                     /translation="MSSTAYATPEQEALEAALFEAKRILVGQDRMLERLFVSWLAKGH
                     VLLEGAPGLAKTLAAETMATILGGSFNRIQFTPDLVPADLVGTRVYRPSSETFDVELG
                     PVFANVVLADEINRAPAKVQSALLEVMAERHVSIGGTTHPVPEPFLVMATQNPIESDG
                     VYPLPEAQRDRFLMKVLVDYPDHREETEIARRMTAHRPVPQQVLTEAQVLHLQRKATD
                     VFVHDAVLDYAVRLVLATREPAQHGLAELAPVIANGASPRATLALLAGGRALALMRGR
                     GYVVPRDIYDVSRDVLRHRILLTFDAIADDVDVEAVVERVVSTVLAPRVTPSQDVVED
                     QGVWAA"
     gene            complement(4457..4765)
                     /locus_tag="VNQ33_12155"
     CDS             complement(4457..4765)
                     /locus_tag="VNQ33_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98906.1"
                     /translation="MSNPLTKYLAAAAVAGTVAFGGYTIAAAQDGGSTTTTSPPATQG
                     EQQAPQAGERPERTQPDRGRAGGCDHDQGEAGSGSAPEQAPQTPQTESSNTPSGFTST
                     "
     gene            complement(4895..>5703)
                     /locus_tag="VNQ33_12160"
     CDS             complement(4895..>5703)
                     /locus_tag="VNQ33_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98907.1"
                     /translation="CIRLGRFSNPSQTYGGAALGRPIGGSQPADSRTALNNTQAFVSS
                     WRAKPAPFTSWSRFVAQQYKDFLGRSPSSSESSSAVNALNTGVTTPPAYVQGLLDGAF
                     GSSYGPVARLYFAYFVRSPDKGGLDFWAKRHKGGMKLSAISSNFAASSEFKNKYGSLT
                     NRQFVEKIYNNLFERTGDPSGVTYWTAKLDQKVKTRGAVMIGFSESSEFKRVRAEEIS
                     VVLLYRSLLGRSATPTEFADQVTRLQGGTTVQRLILEIVDSSEYRGRVVT"
BASE COUNT          711 a         1821 c         2315 g          856 t
ORIGIN      
        1 tggcggttcc cggcgaggcg ctcacgtccg ggtcggtcga tgtcgatggc cggctgctgg
       61 gcgtcgacaa cccggtcgcc ttcgaggcgt cggcctcgca accgccgtac ggcctggtcg
      121 tcatggccct gctgctcgtc ggcaccgtga tcgccctggc ggtccggcgc cgacgccgcc
      181 ggggtcgagc gggttccgtc gccgacaccg gcttcgtcgc tccctgatgg tcgtcgtcag
      241 gtcgccatgg cgcttccacc atcggcaccg tcccaggccg gcggaaggcg cgggacgtgc
      301 cgatggtggt cccccgcccc cagggggaga ggtgctggac cctgggaccc gtgatcaggt
      361 gagccgcggg gtccaggcgg tggtggccac gatggcgagc ccgagcaggg cggcggccag
      421 cagcgagagc gggacggtga gctcctggtg ctcggtggcg gtgccgatcg tcgtgccgat
      481 ctggccgtag acgctcgaga gctcctcgct ggtggaggcg gtgtagtcgg tgccgccggt
      541 cttgtcggcg acggcggcga tggccgcggg gtcggccccg gcggccatgg tcttgccgtc
      601 gaccacgatg gtggcgtcgg ccgtgccgta ggcgatggtg aacaccggga tcttctcgtc
      661 gacggcgcca tcggcggcct ggtcgagcgt cgtaccggtg gagctgtcgc cgtcggccag
      721 gaggatgatg gcccggtagg tgtcgtcgtc ggcggtggcg ccctcggcgg ctgccgcggc
      781 ggcgtcggcc ttcagcacgt cgacggacgt cttgagcccg tcaccggcgg ccgtgccgtt
      841 ggcggcctcc aggccgtcga tggccgacag cagctcggcg cggtcggtgg tcggcgaggc
      901 gagggtgtgg gtctgggcat cgaacgtgac gaggccgatc cggtagccct cgggcgccga
      961 ttccacgaac tccttggccg ccttctgcgc ggcggcgagg cgggtggggg cgacatcggt
     1021 ggcggtcatc gacttggaca cgtcgagggt gagcatcacg acggcctcct tgcgctcgac
     1081 cgtcacggcg tgggccggtc gggcgagccc gacgaccatc gcggtgaggg cgagcacgag
     1141 ggcgaacgcc gtgaggtggc ggcgccaacc gccccacctc ggggcgaagc tgtcgatgag
     1201 ggccaggtcg gggtggcgga cagccggggc cgtgcggcgg tgctgggcca ccacgtaggc
     1261 ggcgaccagt ccggcgacgg cgacgagcag ccagagccgg ttgggggaga tgagcgtcat
     1321 ggttctctcg gatccttcag cgatcggtct cagagcggtc gggtgctggt cgccacggcg
     1381 atgccgccgg cggcgaggag caccacgaag gcgccgccga gcagtgcccg gcccacctcc
     1441 cggggctcgg tctcgtagcc gatggtggtc tggatgtcgg cgtagacgtc cttgagctcg
     1501 ccggccgagg tggcctggaa ggaggtgccg ccggtggcgt cggccacggc ctccatggtc
     1561 cgggcgtcgg ccgggaccgc catcgtctgg ccttcgatcg tcacggtgcc ggtgggggtg
     1621 ccgtaggcga tcgtgctcac cgggacctcg gcgtcgacgg cggcccgggt ggccgagtcg
     1681 accgacctgc ccacggtctc gaccccgtcg gacagcagca cgatcgccgc gggcgccggg
     1741 tcgtcgccct tggcggtgtc ctcgtcgagg gcggtcttca cggcgtcgag cgaggcgaac
     1801 acaccctcgc cggtggcggt gcgggcgttg gtggtgagcg tgctgatggc cgccactacc
     1861 gactggtggt cggtggtggg cgagaccagg agccgggcgt tcgagtcgaa cgacacgagg
     1921 ccgacctcga tgccctcggg cagctcctcc acgaagtcgg tggcgccgtc gatggcggcc
     1981 tcgaggcggg tcgggctgat gtcggtggcc tgcatcgaca gcgacgtgtc gatggcgagg
     2041 acgacgacgc ccctctcgcg gggcacccgc tgctcgcggg tcggctgggc ggccgacacg
     2101 agcacgagcg acagcgctcc gagggcgagc gcgggggcga cgtggcgccg cacgccgcgg
     2161 gcgcgctccg gtgcgatctt gtcgtggagg tggggctggg cgtaccgggc gatggccccg
     2221 gtgcggcggt gccacacggc tgcgtacccg gcggcgagca ggacgacggc gccgagtagc
     2281 aggagccgtt cgggatggag gaagctcatc ggcggcgcct cctggcggcg atgtggcgga
     2341 cgacgtcgcg cagccagtcc cggtcggtgg agacccggag gtgctgggcg cgtgagcggc
     2401 ggagccgctc gtcgatggtg gagcggcgct cggccgcggc ggcggcgtag cgctcgcgga
     2461 acctcttgct ggcggtctgc acctcgcggc gctggccggt ctcggggtcg accagggcga
     2521 gcatgccgac gttcgggatc tcgtcctccc gggggtcggt cacgtgggcg acgatcacgt
     2581 cgtggcgggc ggcgagggag cggagctcgg tgggccagtc ggagccgtcg agcaggtcgc
     2641 tcaccacgat gaccaggccc cgcttgcggg cgagcagcag ggtcttgcgg agggcggcgg
     2701 cgagcgtggc gccctcaccg gtggtgcgcc cacggcgctg gaggcgcacc accagatcga
     2761 ggacggccga gcgtccggca cggggcggga ccacccaggt ctcggcgccg tcggccacga
     2821 cggcgccgat gcggttgccg gcgcggacga cgaggaaccc gaaggcaccg accaccgagg
     2881 cggcgaggtc ccgcttctcc tggttggcgg tgccgtagtc gaggctggcg ctgccgtcga
     2941 tgacgagcca cgtctcgagc tcgcgctcgg cgatcgtgtc gcgcacgtgg acgtcgccgg
     3001 accgggccgt caggttccag tcgatgcggc ggacgtcgtc gcccggctgg taccgccgtc
     3061 cttcggcgcc ttcgctgccg gggccgaggg cgatcgactg gtggtcgccg tggatgaggc
     3121 cgtccagctt gcggttgatc gtcagctcga gcaggcggag ggaccgctcc gacgacgcca
     3181 gcgctgcggt ggcggtcatg ccgcccagac gccctggtcc tcgacgacgt cctggctcgg
     3241 ggtgacccgg ggggccagca cggtgctgac gacgcgctcg accacggctt cgacgtcgac
     3301 gtcgtcggcg atggcgtcga acgtgaggag gatgcggtgg cgcaggacgt cgcgggacac
     3361 gtcgtagatg tcgcggggga cgacgtagcc gcggccgcgc atgagggcga gggctcggcc
     3421 gccggccagg agggcgaggg tggcgcgggg actggcgccg ttggcgatca ccggggccag
     3481 ttcggcgagg ccgtgctggg ccggctcacg cgtggcgagc acgaggcgca ccgcgtagtc
     3541 gagcacggcg tcgtggacga acacgtcggt ggccttgcgc tgcaggtgca gcacctgggc
     3601 ctcggtgagc acctgctggg ggacgggtcg gtgggcggtc atgcgccggg cgatctcggt
     3661 ctcctcgcgg tggtccgggt agtccacgag gaccttcatc aagaaccggt cccgctgggc
     3721 ctcgggcagc gggtacacgc cgtcgctctc gatcgggttc tgggtggcca tgaccaagaa
     3781 gggctcgggg accgggtggg tggtgccgcc gatcgagacg tggcgctcgg ccatcacctc
     3841 gaggagggcg gactggacct tggccggcgc ccggttgatc tcgtcggcga gcacgacgtt
     3901 ggcgaacacc gggccgagct ccacgtcgaa cgtctcgctc gaggggcggt acacccgggt
     3961 gcccacgagg tcggcgggca ccaggtccgg ggtgaactgg atgcggttga agctgccgcc
     4021 caggatcgtc gccatcgtct cggcggccag ggtcttggcc aagccgggtg cgccctcgag
     4081 gaggacgtgg cccttggcca gccaggagac gaagaggcgc tcgagcatcc ggtcctggcc
     4141 gacgaggatg cgcttggcct cgaacagcgc agcctcgagg gcttcttgct cgggggtggc
     4201 gtaggcggtg ctggacatcg ggcatctgct cctgggtggt gggatcggcg gccgtcggct
     4261 cgctgcgggg ttcggtcctg cccatcttct ggacgcgggt gaaaggtcct cgaagcagac
     4321 gctgggagct tcctgagaat cgccaccgaa gcctgggcat cgcctgagag ccacgtggcg
     4381 gaaccgtgcc gacgcggacc agcccgcccc gagggcaggg gcgggctggt ccggctgtgg
     4441 cggtcgaggg ggcgggtcag gtgctggtga agcccgacgg ggtgttcgac gactctgtct
     4501 ggggcgtctg gggggcctgc tcgggagccg atcccgaacc ggcttcgccc tggtcgtggt
     4561 cgcagcctcc ggcccggccg cggtcgggct gggtgcgctc gggccgctcg ccggcctgcg
     4621 gggcctgctg ttcgccctgg gtggcgggcg ggctcgtggt ggtggtcgac ccgccgtctt
     4681 gggcggcggc gatcgtgtag ccgccgaagg ccacggtgcc ggcgaccgcc gcggcggcga
     4741 ggtacttggt gagggggttg gacatggagg gatgctcctg tggtggtcgg atggggagca
     4801 ctcgccaccc aagcagaccc gtctccgagg atcccctgcg caaccgcagc gttccctggc
     4861 agccgcagag cggcgtcgtc cgccggtcgg gcgcctacgt gaccacgcgg ccgcggtact
     4921 ccgacgagtc gacgatctcg aggatgagcc gctggaccgt ggtcccgcct tggaggcggg
     4981 tcacctggtc ggcgaactcg gtgggggtcg ccgagcggcc gaggagcgag cggtagagca
     5041 gcacgacgct gatctcctcg gcccggaccc gcttgaactc gctggactcg gagaagccga
     5101 tcatgaccgc cccgcgggtc ttcaccttct ggtcgagctt ggcggtccag taggtgacgc
     5161 cggacgggtc gccggtgcgc tcgaagaggt tgttgtagat cttctcgacg aactgccggt
     5221 tcgtgagcga gccgtacttg ttcttgaact cgctcgacgc cgcgaagttg ctggagatgg
     5281 cgctgagctt catcccgccc ttgtgccgct tcgcccagaa gtccagaccg cccttgtcgg
     5341 gggagcggac gaagtacgcg aagtagaggc gggcgacggg gccgtagctg gagccgaagg
     5401 ccccgtcgag caggccctgg acgtaggccg gcggggtggt gacgccggtg ttcagggcgt
     5461 tgacggccga ggacgactcg ctgctcgacg gcgagcggcc gaggaagtcc ttgtactgct
     5521 gggcgacgaa cctcgaccac gaggtgaacg gcgccggctt cgcccgccag ctggacacga
     5581 aggcctgcgt gttgttcagc gccgtgcggc tgtcggcggg ctgcgagccg ccgatggggc
     5641 ggccgagggc ggcgccgccg taggtctggc tggggttcga gaaccgaccc agacggatgc
     5701 agg
//
LOCUS       DATMFS010000635         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_91835, whole genome shotgun sequence.
ACCESSION   DATMFS010000635 DATMFS010000000
VERSION     DATMFS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91835"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VNQ33_12165"
     CDS             <1..560
                     /locus_tag="VNQ33_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ98908.1"
                     /translation="PLLDAGTPLLAVLGNHDVKRGRALAQVEALGMPGRWWARRIGDV
                     AIVGLDSTRIDHPDQRAFLDEALAADATWKVVLAHHPPYSAGYQGSHRATRRLVAPLA
                     ARHGVQLVLSGHDHDYQRSHPIEGTTYVVTGAAAVKRLTGTRSFTAASFSAHHFVDLS
                     FTADRLVLRAITADLQIADEATIVP"
     gene            578..1996
                     /locus_tag="VNQ33_12170"
     CDS             578..1996
                     /locus_tag="VNQ33_12170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWJ98909.1"
                     /translation="MLPIPQRLVEQIERRLRQRRVHGLALLAFDLDGPRFGGGVGFAD
                     LDRGEPVTTETVFRVASVSKQLTTSVVLKAVGDGLLDLDQPVNDHLPVAQHLTDAEGR
                     PAPVPLRTLLSHSSGLPSSIRGADLGRPLLTRLANGAVPASLADAVTGLRLERRPGER
                     IVYANSGLNVAGFLAGLAFGTSFEDAARDEVLAPLGMRRSEFDTRRSGPGVATPYGSI
                     APPTVSSKPATAMQLIATPMGGLTSTVEDLARFGRMVLGGGALEGDRILDTGLVAAAT
                     TLEITNHPDLEQGYGLGFKVRRWRGRRLVGHDGNMPGVAANVWLAPDDGVGVVVLTNG
                     FALGIPHEVALLAIDELLGPAAVEEEPAAPPSPAEVSELEAFGRRVQGRYHVDGVAPP
                     GIVGTLSRLTTKVQVIHEARGLLRVEGNPGSDGPMWLRPDGTPGRYRVSAPVDHGTNA
                     VFDDRPDGLRLWISQATLLRPA"
BASE COUNT          258 a          843 c          704 g          273 t
ORIGIN      
        1 ctcccctgct cgacgccggc accccgctgc tcgccgtctt gggcaaccac gacgtgaagc
       61 gcggacgggc gctcgcccag gtggaggcgc tcggcatgcc cggccgctgg tgggcgcggc
      121 gcatcggcga cgtggcgatc gtcgggctcg actcgacccg catcgaccac ccggaccagc
      181 gcgccttcct cgacgaggcg ctggccgccg acgccacgtg gaaggtcgtc ctcgcccacc
      241 acccgccgta ctcggccggc taccagggct cgcaccgggc cacccgccgc ctcgtcgctc
      301 cgctcgccgc ccgccacggc gtccagctcg tgctgtcggg ccacgaccac gactaccagc
      361 ggtcgcaccc gatcgagggc acgacctacg tcgtgaccgg cgccgccgcc gtgaagcgcc
      421 tcaccgggac gcggtccttc accgccgcct cgttctcggc ccaccacttc gtggacctgt
      481 cgttcaccgc cgaccgcctc gtcctccggg cgatcaccgc cgatctccag atcgccgacg
      541 aggccaccat cgtgccgtag gtcggtatcg tcaccgggtg ctgccgatcc cccaacgcct
      601 cgtcgagcag atcgagcggc ggctccggca gcggcgggtg cacggcctcg ccctgctggc
      661 gttcgacctc gatgggcccc gcttcggcgg gggcgtcggg ttcgccgacc tcgatcgcgg
      721 cgagccggtc acgaccgaga ccgtgttccg cgtcgcctcc gtctccaagc agctcaccac
      781 ctcggtggtc ctgaaggccg tgggcgacgg gctcctcgac ctcgaccaac cggtcaacga
      841 ccacctgcct gtggcgcagc acctcaccga tgccgagggc cgacccgcgc cggtgccgct
      901 gcgcaccctc ctctcgcact cgtcgggcct cccgtcgagc atccggggcg ccgacctcgg
      961 ccgaccgctg ctcacccggc tcgcgaacgg ggccgtcccg gcgtcgctgg ctgatgcggt
     1021 gacgggcctg cgcctcgaac gccgccccgg tgagcgcatc gtgtacgcca actcgggcct
     1081 gaacgtggcc ggcttcctcg ccgggctggc cttcggcacg tccttcgagg acgcggctcg
     1141 agacgaggtc ctggcgccgc tcgggatgag gcggtccgag ttcgacaccc gccgcagcgg
     1201 ccccggcgtg gccacgccct acggctccat cgctccgccg acggtgagct cgaagccggc
     1261 gacggcgatg cagctcatcg ccaccccgat gggcgggctc acctccaccg tggaggacct
     1321 cgcccggttc ggccggatgg tcctgggcgg tggcgccctc gagggcgacc gcatcctcga
     1381 cacgggcctg gtggcggccg cgaccacgct cgagatcacc aaccaccccg atctggagca
     1441 gggctacggg ctcgggttca aggtccggcg gtggcggggg cgtcggctgg tcggccacga
     1501 cggcaacatg cccggcgtgg ccgccaacgt ctggctggct cccgacgacg gcgtcggcgt
     1561 ggtggtgctc accaacggct tcgccctcgg catcccccac gaggtggccc tgctggccat
     1621 cgacgagctc ctcggcccgg cagccgtcga ggaggagccg gcggcgccgc cgagcccggc
     1681 cgaggtgagc gagctggagg cgttcgggcg ccgggtccag ggccgctacc acgtggacgg
     1741 cgtcgcgcca cccggcatcg tgggcacgct ctcccggctg accacgaagg tgcaggtcat
     1801 ccacgaggcg cgcggcctgc tgcgggtcga gggcaacccg ggctccgacg gcccgatgtg
     1861 gctccgcccc gacggcaccc ccggccgcta ccgggtctcc gcacccgtcg accacggcac
     1921 caacgccgtc ttcgacgacc gccccgacgg cctccgcctc tggatcagcc aggccaccct
     1981 cctccgcccc gcctgaacgg ccgccgaccc gccgagcggg tgcgccaggc cgacggaccc
     2041 aaggcccagc accggcgatg cggtcccgac gatccggt
//
LOCUS       DATMFS010000636         5822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_67694, whole genome shotgun sequence.
ACCESSION   DATMFS010000636 DATMFS010000000
VERSION     DATMFS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67694"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1023)
                     /locus_tag="VNQ33_12175"
     CDS             complement(175..1023)
                     /locus_tag="VNQ33_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ98910.1"
                     /translation="MTALPAEPDRRADRAGIDWSAVRSIVRRDLKAAGRSKAMVLPML
                     LVPLLMLVLLPMSVGLAAQGDRLDPTTFLTMVPEQLAAPVSRAPQHEQLVILVLGYLV
                     APLFLIVPLMVSAVLAGDAFAGEKERKTLETILHLPVRDKDLYIGKLLVGFVPSVAVS
                     WIGFLLFCITANLSAWPVMHRAFMPTKLWTLMIFWIAPGVAAVGLGVMVRVSVRVNNT
                     QEAQQLGGAVVLPLVILAVAQTTALLLAGLVPTFVAGCVVWAIAIWLNLRGAQSFTRD
                     RMAARL"
     gene            complement(1020..1997)
                     /locus_tag="VNQ33_12180"
     CDS             complement(1020..1997)
                     /locus_tag="VNQ33_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ98911.1"
                     /translation="MGAPQRVIDCQGLTRNFPSGRALDHLTLDAGRGEVLALLGPNGA
                     GKTTTMRLLNGVLLPHDGTAQVLGLDPWRQGEELRRRTGVLTENAGLDERFTALENLE
                     FVGHLRGMSSSEARARGRTLLDRFDMGHRADVKVQGFSTGQRKRVALARALLHDPELL
                     FLDEPTSGLDPAATRDVVDLIGSLAAEGRTVVLCTHFLGEAGKLASRMAVLFHGVLHA
                     FGTPEDLAAELWSRFEVRLDLGAPAAPPLIAALLALPQVHDASPTDAGALVFTTERAD
                     VPRVVATCVAHGSPVYGVDPRDPNLEDVYFAIEARAGAPATAAGEEVPA"
     gene            2109..3599
                     /locus_tag="VNQ33_12185"
     CDS             2109..3599
                     /locus_tag="VNQ33_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ98912.1"
                     /translation="MNLASIIDPHPDDATALISRGRFTTYGELREQVGSLRAGLIGLG
                     IDPGDRIGIVCANNWYFVASYLAVLGAGAVAVPLNPLSPPRELERELAAVGARAMLVG
                     PSGRSNADGIDRSRLPVLEFVVHSEGTSTEGAILLDGLLATPPVPVVDRAADDLAVLI
                     FTSGTAGSPKAAMLSHGNLLANLEQTIALRGQGDATDVGFGVLPMFHIFGLNVVLGTA
                     LHAGASVLLVERFDPMSAVEAIQKHGVTVLPGAPQMWAAWAAMPGLPEDSFATVKVAT
                     SGAAHLDPHVAETIRSRFGLEVGEGYGLTEASPVVTASIGLPWRPGSVGAPVDGVDVR
                     LVDLDGEDVLVGDPGEIWVKGPNVFQGYWNDAEATRACIDDDGWLHTGDIAMVDDEGF
                     LFLVDRAKDLIIVSGFNVFPAEVEEVLLEMDGIREVAVVGVPHPHTGEAVKAFVVVEP
                     GHSLEEDSIIEFAAQRLARYKCPQKVNFVDELPLNLSGKVLRRALR"
     gene            3677..4321
                     /locus_tag="VNQ33_12190"
     CDS             3677..4321
                     /locus_tag="VNQ33_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWJ98913.1"
                     /translation="MPDRARHIPEATVARLPVYLRSLAELVDEKIPTVSSERLAEMAG
                     VNAAKVRKDLSYLGSYGTRGVGYDVEYLLFQMSRELGLTHDWPVAIVGAGNLGSALAN
                     YGGFTQRGFPVAALFDSDKAKVGQLIHGVEVHHTDDIAPLAKELKIGIGVIATPASAA
                     QDAADKLVQAGVGSILNFAPTVISVPDGVSLRKVDLALELQILSFYQEHRDLGS"
     gene            4380..4940
                     /locus_tag="VNQ33_12195"
     CDS             4380..4940
                     /locus_tag="VNQ33_12195"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWJ98914.1"
                     /translation="MSDLPATDAGPAAEAGPVDPDVDHIIGVSFDKVTRAEEVLLALV
                     HLQQEGEIALSDAVVVTKDDGGKVRIHQTIDPTPGRSALTGSIWGMLVGALFGGPVFL
                     AAAAVGAGGGALMAKLVDLGLDDAWVRDVGHWLEPGTSALLILVAAEVRPAVLRELGR
                     YEGDVLYCTFPGAVREELERALSDEA"
     gene            4944..5591
                     /locus_tag="VNQ33_12200"
     CDS             4944..5591
                     /locus_tag="VNQ33_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWJ98915.1"
                     /translation="MSPRYPVMVDLAGRPCLVVGGGEVATRKVLGLLASEALVTVRAP
                     ELSAPLAQLAGAGRIRWEEQTYTGLGVADDGRPWSLVLAATDDVAVNRRVGIEAAAVG
                     AWANDATDPTGGAAAVPASWHGGPVTIAVSTDGVHPGAARWLCDQAALTVTPEALAAL
                     DLVDEMRLDDVAGGGLGRRPDWRLAVESGTLELIREGHLAEAKERLQACLSSSSD"
     gene            5603..>5822
                     /locus_tag="VNQ33_12205"
     CDS             5603..>5822
                     /locus_tag="VNQ33_12205"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98916.1"
                     /translation="MPLDLFERMTIDGARLPKALHDLNEREHIGEVVVLSTCNRTEVY
                     AIAERFHGAYADVRNFLSELAFVPPDEFA"
BASE COUNT          835 a         2112 c         2076 g          799 t
ORIGIN      
        1 atcacccgca cctcgggggc gagctggcgt atcacggagt gcacgacccc ggcgaactcg
       61 tcgacggtcc cgtagtcggt caggaaggag atggtgtcga agcggcccac aggcctccga
      121 ccctacgggc tggccccgaa ccggcgacag gcgaggcccg ccggcgcggc gtcgtcagag
      181 gcgggcggcc atgcggtcgc gggtgaacga ctgcgcgccg cggaggttca gccagatggc
      241 gatcgcccac accacgcagc cggccacgaa cgtgggcacc aacccggcga gcaggagcgc
      301 ggtggtctgg gcgacggcga ggatcacgag gggcagcacg acggccccgc cgagctgctg
      361 ggcctcctgc gtgttgttga cgcgcaccga cacccgcacc atcaccccga ggccgacggc
      421 agccacgccg ggcgcgatcc agaagatcat gagcgtccac agcttcgtgg gcatgaaggc
      481 gcggtgcatc accggccagg ccgacaggtt ggcggtgatg cagaagagga ggaagccgat
      541 ccacgagacg gcgaccgacg gcacgaagcc gacgaggagc ttgccgatgt agaggtcctt
      601 gtcccgcacc gggaggtgca ggatcgtctc gagcgtcttg cgctccttct cacccgcgaa
      661 cgcgtcgccg gccaagacgg cggagaccat gagcgggacg atcaggaaca gcggtgccac
      721 caggtacccg aggacgagga tcaccagctg ctcgtgctgg ggcgcgcggg agacgggtgc
      781 ggccagctgc tcggggacca tcgtgaggaa cgtggtcggg tcgaggcggt cgccctgcgc
      841 cgccaggccg accgacatgg gcaacaagac gagcatcagg agggggacga gcagcatcgg
      901 caggaccatg gccttcgagc gcccggccgc cttcaggtcc cggcgcacga tcgagcgcac
      961 ggcgctccag tcgatgccgg cgcggtcggc gcggcggtcg ggctcggcgg gcagcgcggt
     1021 catgccggca cctcctcgcc ggcggcggtg gcgggagcac cagcgcgggc ctcgatcgcg
     1081 aagtagacgt cctccaggtt cgggtcccgg gggtcgacgc cgtagaccgg cgagccgtgg
     1141 gccacgcagg tggcgaccac ccgggggacg tcggcgcgct cggtggtgaa caccagcgcg
     1201 ccggcatcgg tgggcgacgc gtcgtggacc tggggaagcg ccagcagggc ggcgatgagc
     1261 ggcggggccg cgggtgcgcc gaggtcgagg cggacctcga agcgcgacca cagctcggcg
     1321 gcgaggtcct cgggggtgcc gaaggcgtgg aggacgccgt ggaagagcac ggccattcgg
     1381 ctggcgagct tgccggcctc gccgaggaag tgggtgcaca acacgacggt ccggccctcc
     1441 gccgccagcg agccgatgag gtcgaccacg tcgcgcgtcg ccgccggatc caacccggag
     1501 gtcggctcgt cgaggaacag cagctccgga tcgtggagca gcgcccgggc gagggccacc
     1561 cgcttgcgct gcccggtgga gaacccctgc accttcacgt cggcccggtg ccccatgtcg
     1621 aaccgatcga gcagggtccg gccccgcgcc cgggcctccg acgacgacat cccgcgcagg
     1681 tggccgacga actccaggtt ctcgagcgcc gtgaagcgct cgtcgaggcc ggcgttctcg
     1741 gtgagcacgc cggtgcggcg tcgcagctcc tcgccctgcc gccagggatc gaggcccagg
     1801 acctgcgccg tgccgtcgtg cggcaggagg acgccgttga gcaggcgcat cgtcgtggtc
     1861 ttgccggcgc cgttcggccc cagcagggcg agcacctcgc cgcgcccggc atcgagggtc
     1921 aggtggtcga gggcacggcc ggaggggaag ttccgggtca gcccctggca gtcgatgacc
     1981 cgttgcggcg cccccatcgc cctcagtgtg ccatcgagcc ggccgaacgc ggatcggacg
     2041 cgcgggcacc aggtgtgctg cactggtaga cggccgttcc ggcccgcgtg ccaccacctg
     2101 aggtctctgt gaatctcgcc tcgatcatcg acccccatcc cgacgacgcc accgccctca
     2161 tcagccgggg gcgcttcacc acctacggcg agctgcgcga gcaggtcggc agccttcgtg
     2221 cggggctcat cgggctcggg atcgacccgg gtgaccgcat cgggatcgtg tgcgccaaca
     2281 actggtactt cgtggcgtcc tacctcgccg tcctcggcgc cggcgccgtc gctgttcccc
     2341 tcaacccgct cagcccgccc cgcgagctcg agcgcgagct ggccgccgtg ggcgcccggg
     2401 cgatgctggt cggtccgtcg ggccggtcca acgccgacgg catcgaccgc agccggctgc
     2461 ccgtcctcga gttcgtcgtg cacagcgagg ggaccagcac cgagggcgcc atcctcctcg
     2521 acggcctgct ggccaccccg ccggtgccgg tcgtcgatcg agcggccgac gacctcgccg
     2581 tgctgatctt caccagcgga acggccgggt cgccgaaggc ggcgatgctc tcgcacggca
     2641 acctgctcgc caacctcgag cagaccatcg ccctgcgggg ccagggcgac gccaccgacg
     2701 tgggcttcgg ggtgctgccg atgttccaca tcttcggcct caacgtcgtg ttgggcacgg
     2761 ccctgcacgc cggtgcttcg gtgctgctgg tcgagcgctt cgatccgatg tcggcggtcg
     2821 aggccatcca gaagcacggc gtcaccgtgc tccccggcgc cccgcagatg tgggcggcct
     2881 gggccgccat gccggggctg cccgaggaca gcttcgccac cgtgaaggtc gccacctccg
     2941 gcgccgccca cctcgatccg cacgtggctg agaccatccg gtcccgcttc ggcctcgagg
     3001 tcggcgaggg ctacgggctc accgaggcat cgcccgtcgt caccgcctcc atcgggctgc
     3061 cctggcgccc gggttcggtc ggcgcgccgg tcgacggggt cgacgtgcgc ttggtcgacc
     3121 tggacggcga ggacgtcctc gtcggcgacc ccggcgagat ctgggtcaag ggcccgaacg
     3181 tcttccaggg gtactggaac gacgccgagg ccacccgggc ctgcatcgac gacgacgggt
     3241 ggctccacac cggtgacatc gccatggtcg acgacgaggg cttcttgttc ctggtcgacc
     3301 gggccaagga cctcatcatc gtgtcgggct tcaacgtctt cccggccgag gtcgaagagg
     3361 tgctgctgga gatggacggc atccgcgagg tcgccgtcgt cggcgtcccg cacccccaca
     3421 cgggtgaggc cgtcaaggcc ttcgtggtgg tcgagccggg ccactcgctg gaggaggact
     3481 cgatcatcga gttcgcggcc cagcgcctcg cccggtacaa gtgcccccag aaggtgaact
     3541 tcgtcgacga gctcccgctg aacctcagcg gcaaggtcct ccgccgagcc ctccgctagc
     3601 cggaccctgg tccggcaccg gccggagtgg gcctgcctcg ctcgcttgtg aacttgcgca
     3661 ctagcgtttc gggtgcatgc ccgaccgcgc ccgacacatc cccgaggcca ccgtcgcccg
     3721 gctgccggtg tacctccgga gcctggccga gctggtggac gagaagatcc cgacggtgtc
     3781 gtcggagcgg ctggcggaga tggccggcgt caacgccgcc aaggtgcgca aggacctgag
     3841 ctacctcggg tcctacggga cccgaggggt cggctacgac gtcgagtacc tgctgttcca
     3901 gatgagccgg gaactggggc tcacccacga ctggcccgtc gccatcgtcg gtgccggcaa
     3961 cctcggcagc gccctcgcca actacggcgg cttcacccag cgcggcttcc cggtggcggc
     4021 cctgttcgac tcggacaagg ccaaggtcgg ccagttgatc cacggcgtcg aggtccacca
     4081 caccgacgac atcgccccgc tggccaagga gctcaagatc ggcatcggcg tcatcgccac
     4141 cccggcctcc gcggcccaag acgccgcgga caagctcgtg caggcgggcg tcgggtcgat
     4201 cctgaacttc gcgcccaccg tcatctcggt ccccgacggc gtctcgctcc gcaaggtcga
     4261 cctcgccctg gagctgcaga tcctgagctt ctaccaagag caccgcgacc tcggctcctg
     4321 aacgccggcg tcgcgcctcg cgggtcccga tcgggcaccc cgtcggtagg ctcgacccga
     4381 tgagcgacct ccccgcgacc gatgccggac ctgccgcgga agccggtccc gtcgaccccg
     4441 acgtcgacca catcatcggg gtgtcgttcg acaaggtgac ccgggccgag gaggtcctcc
     4501 tcgccctcgt ccacctgcag caggagggcg agatcgcgct gtccgacgcc gtcgtcgtga
     4561 ccaaggacga cggcggcaag gtccgcatcc accagaccat cgacccgaca ccgggccgca
     4621 gcgcgctcac cgggtcgatc tgggggatgc tcgtcggcgc cctcttcggc ggcccggtgt
     4681 tcctcgccgc cgcggcggtg ggtgccggcg gcggcgccct gatggccaag ctcgtcgacc
     4741 tcggcctcga cgacgcctgg gtgcgcgacg tcggccactg gctcgaacca ggcacctcgg
     4801 cgctgctcat cctggtggcg gccgaggtcc gcccggcggt gctgcgcgag ctggggcgct
     4861 acgagggcga cgtcctctac tgcacgttcc ccggcgcggt ccgcgaggag ctcgagcgcg
     4921 ccctgagcga cgaggcctga gacatgtcgc cccgctatcc cgtgatggtc gacctggccg
     4981 gccggccgtg cctggtggtc ggcgggggcg aggtcgccac gcgcaaggtg ctcggcctgc
     5041 tcgcgtccga agcgctcgtg acggtacggg cccccgagct cagcgcgccc ctcgcccagc
     5101 tggccggtgc cggccgcatc cggtgggagg agcagacgta caccggcttg ggcgtcgccg
     5161 acgacggccg gccgtggagc ctggtgctgg ccgccaccga cgacgtcgcc gtgaaccgcc
     5221 gcgtcggcat cgaggccgcc gccgtgggcg cgtgggccaa cgatgccacc gaccccacgg
     5281 gcggtgcggc cgcggtcccg gcgagctggc acggcggccc ggtgacgatc gcggtgtcca
     5341 ccgacggcgt ccaccccggc gccgcccgct ggctgtgcga ccaggcggcc ctgaccgtca
     5401 cccccgaagc cctggccgcc ttggacctcg tcgacgagat gcggctcgac gatgtcgctg
     5461 gtggcgggct cggccgccgc ccggattggc ggctggccgt cgagtcggga acacttgagc
     5521 tcattcgtga aggccatctg gccgaagcca aggagcgcct gcaggcgtgt ctgtcgtcgt
     5581 catcggactg aaccaccgga ccatgccgct cgatcttttc gagcggatga ccatcgacgg
     5641 cgcgcgcctg cccaaggccc tgcacgatct gaacgagcgc gagcacatcg gcgaggtcgt
     5701 cgtcctctcc acgtgcaacc gcaccgaggt ctacgccatc gccgagcggt tccacggcgc
     5761 ctacgccgat gtccgcaact tcctctccga gctggcgttc gtgccgcccg acgagttcgc
     5821 tg
//
LOCUS       DATMFS010000637         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21918, whole genome shotgun sequence.
ACCESSION   DATMFS010000637 DATMFS010000000
VERSION     DATMFS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21918"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VNQ33_12210"
     CDS             <1..442
                     /locus_tag="VNQ33_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98917.1"
                     /translation="ARDAAAANGMSIETRISQDDLARFRTIVTVTGALLGLAIVAMAV
                     GLIRTESARDVRTLTATGASSRSRRAITATTAGALAALGATLGIGGAYLALVAGYHAD
                     LSQLLPLPWIQLATIAVGLPVLATAAGWLLAGRDPSGFSRQTFD"
     gene            629..1942
                     /locus_tag="VNQ33_12215"
     CDS             629..1942
                     /locus_tag="VNQ33_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ98918.1"
                     /translation="MAHLGGLRSGASVVAVNSHLAAPEAAYQLTTGDVALVIAGPATA
                     DRAREAAAVDGRCEVHTWGGDGWDDWLASAPAGPPPDDVAIVPNLLFTSGTTGTPKAT
                     HLPPNVFPPRASWAEFVEAARANRFVGLGRHLVVAPLHHTGPLNAVRALAAGTPIGVL
                     PRFGAERTLQAIDEWAIASTTVVPTHLARLAALPSEVRDRYEVSSLRLVFLTGAACPV
                     DVKRAIIDWWGPVVLEAYGATEVGVTASITSEDWLARPGSVGRSVAPYEAVVFDAEGR
                     AVPSGTEGRLYFADRTGRGIVYEGDPERTAAAHIAPGVFTLGEIGRVDEDGFVFITDR
                     FSDMVVTGGVNVYPAEMEQVLVAHPGVADVAGIGLPHPDLGEQLVALVVPSDPEAPPM
                     IDDLQAWCEDRLSRFKCPRQIRLVDGLGRTPLGKLDKRSLRAAQG"
     gene            complement(1979..2344)
                     /locus_tag="VNQ33_12220"
     CDS             complement(1979..2344)
                     /locus_tag="VNQ33_12220"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWJ98919.1"
                     /translation="MAEQPARSGMLGGMTVIKINAITVPAESGDELAQRFAARAGAVD
                     GAEGFEGFELLKPTDERLQWLVVTRWRDQESFEAWTSSQAFAHGHQGAAKPAAHGGPV
                     STASELWSYEVAGGSSAQA"
     gene            2343..>2792
                     /locus_tag="VNQ33_12225"
     CDS             2343..>2792
                     /locus_tag="VNQ33_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98920.1"
                     /translation="MGSSYANVTVVGAGVDAVREVLAGTGAHLAAVGGDTVVFSPGHE
                     EPLGGDELAIRLATSLGVPVVDVAVFDSDFALIHVISPAGEIAAVATAPPNGAAIMGE
                     MTGDPSAVPGAGIPTADTAAALVAAVGRGDAAKVKHALEADNVFADES"
BASE COUNT          339 a         1104 c          980 g          369 t
ORIGIN      
        1 agcccgcgac gcggcggccg ccaacggcat gagcatcgag acgcggatct cgcaggacga
       61 cctggcccgg ttccgcacca tcgtgaccgt gaccggcgcc ctgctcggcc tggccatcgt
      121 cgccatggcc gtcgggctca tccgcaccga gtcggcccgc gacgtccgga cgctcacggc
      181 caccggcgcc agcagccgga gccgccgggc catcaccgcg acgaccgcgg gggcgctcgc
      241 cgcgctcgga gccaccctcg gcatcggcgg cgcctacctc gccctcgtcg cgggctacca
      301 cgcggacctc tcgcagctgc tgccgctccc gtggatccag ctcgccacca tcgccgtcgg
      361 cctcccggtc ctggccaccg ccgcgggctg gctcctcgcc ggccgcgatc cgtccggctt
      421 ctcccgccag acgttcgact gaccggccac gggcggccgc tacggtcggc gcccgtgacc
      481 gctccgctcc tcgtcgatcc gcaccggtcg tggtccgcag ccgaggtctg ggacaccgcc
      541 gaccgcatcg cccacctcgc tcgcgccgcc accggcggag gccggcggcg cgtcgccgtg
      601 ttcgccgaga acgccggggg aggcggtcat cgcccacctc ggcgggctca ggtccggcgc
      661 atcggtcgtg gcggtcaaca gccacctcgc cgcccccgaa gccgcctacc agctgaccac
      721 cggcgacgtc gccctcgtca tcgccgggcc ggccaccgcc gaccgggccc gcgaggccgc
      781 ggccgtcgac ggccggtgcg aggtgcacac gtggggcggc gacggctggg acgactggct
      841 ggcgtcggca ccggccggtc ccccgcccga cgacgtggcg atcgtcccga acctgctgtt
      901 cacgtcgggc acgacgggga cgccgaaggc cacgcacctg ccgcccaacg tgttcccacc
      961 gcgggcctcg tgggcggagt tcgtggaggc cgcccgggcc aaccggttcg tcggcctcgg
     1021 ccgccacctc gtggtcgcgc cgctgcacca caccggcccg ctcaacgccg tccgagcgct
     1081 ggcggccggg acgcccatcg gcgtgctgcc ccgcttcggt gccgagcgca ccctccaggc
     1141 gatcgacgag tgggccatcg cctccaccac cgtcgtgccg acgcacctgg cccgcctggc
     1201 cgccctgccg tcggaggtcc gcgaccgcta cgaggtgtcc agcctgcgcc tcgtcttcct
     1261 caccggcgcg gcgtgcccgg tggacgtgaa gcgggcgatc atcgactggt ggggaccggt
     1321 ggtcctcgag gcgtacggcg ccaccgaggt cggcgtcacc gcctccatca ccagcgagga
     1381 ctggctggcc cgccccggct cggtcggccg ctcggtcgcc ccctacgagg ccgtcgtgtt
     1441 cgacgccgag ggacgggccg tcccgagcgg caccgaggga cggctctact tcgccgaccg
     1501 gaccggccgg ggcatcgtct acgaaggcga ccctgagcgc accgcggccg cccacatcgc
     1561 accgggcgtg ttcaccctcg gcgagatcgg gagggtggac gaggacggct tcgtcttcat
     1621 caccgatcgc ttctccgaca tggtcgtgac cggcggcgtg aacgtctacc ccgcggagat
     1681 ggagcaggtc ctcgtcgccc accccggcgt ggccgacgtc gccggcatcg gcctccccca
     1741 ccccgacctc ggcgagcagc tcgtcgccct cgtggtgccg tcggaccccg aggcaccgcc
     1801 gatgatcgac gacctccaag cctggtgcga agaccgcctg tcgaggttca agtgcccgag
     1861 gcagatccgc ctggtggacg gcctcggccg caccccgctg ggcaagctcg acaagcggag
     1921 cctgcgagcc gcccagggct gatcctgcac tcgagcgaga aggcgcccgt gccgctggtc
     1981 aggcctgggc cgaggacccg ccggcgacct cgtagctcca gagctccgag gcggtgctga
     2041 ccgggccgcc gtgcgcggct ggcttggcgg cgccctggtg gccgtgggcg aacgcctgcg
     2101 agctcgtcca cgcctcgaac gactcctggt cgcgccagcg ggtcacgacc agccactgga
     2161 ggcgctcgtc ggtgggcttc aggagctcga agccctcgaa gccctcggct ccgtcgaccg
     2221 ctccggcccg ggcggcgaag cgctgggcga gctcgtcgcc cgactcggcg ggcacggtga
     2281 tggcgttgat cttgatcacg gtcatgccgc ccagcatgcc ggatctggca ggctgctccg
     2341 ccatgggcag ctcctatgcg aacgtgaccg tggtcggggc cggcgtcgat gccgtccggg
     2401 aggtcctggc gggcaccggt gcgcacctcg ccgcggtggg cggcgacacc gtggtcttct
     2461 ctccgggcca cgaggagccg ctcggcggcg acgagctggc gatccggctg gccacctcct
     2521 tgggcgtacc cgtcgtcgac gtcgccgtgt tcgactcgga cttcgcgctc atccacgtca
     2581 tcagccctgc gggcgagatc gccgcggtgg ccacggcgcc cccgaacggg gcggcgatca
     2641 tgggcgagat gaccggcgat ccgtccgccg tgcccggcgc tggcatcccc accgccgaca
     2701 cggcggccgc gctggtggct gcggtggggc gcggcgacgc cgccaaggtc aagcacgccc
     2761 tcgaggccga caacgtcttc gccgacgagt cc
//
LOCUS       DATMFS010000638         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_45498, whole genome shotgun sequence.
ACCESSION   DATMFS010000638 DATMFS010000000
VERSION     DATMFS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45498"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNQ33_12230"
     CDS             complement(<1..254)
                     /locus_tag="VNQ33_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWJ98921.1"
                     /translation="MSIHALLQAFITVFPAELPDKTMIATIVLTTRYRRPLWVWVGAV
                     SAFAIHVVVAVAAGSALSLLPDVLVQLVVAGLFLTGAVLL"
     gene            597..1916
                     /locus_tag="VNQ33_12235"
     CDS             597..1916
                     /locus_tag="VNQ33_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWJ98922.1"
                     /translation="MTALPTSVPSRPRTYVLDTSVLLADPQALERFDEHAVVLPIVVL
                     MELEGKRNHPELGWAARQSLRTLERLRLEHGSLTIPLPVNARGGTLRVELNHQSTAGL
                     PPTMASDNNDHRILAVAHNLRLEGHDVTVVTKDLPLRLKASIVGLHADEYRNELAEDT
                     SWTGFVELDVEPDVIDDLFTHREVDLPEAAELPCNTGVALHAGSQSALARVHADKSLR
                     LIRGDLSLFDVVGRSAEQRIALDVLADPSVSIVSLGGNAGTGKSVMALAAGLEAVLER
                     RTHKRILIFRPLYAVGGQDLGFLPGDKDEKMGPWAGAVFDALESIASPEVVEEVMARN
                     LLEVLPLTHIRGRSLTDTFVIIDEAQNLERPVLLTALSRLGEGSRVVLTHDVAQRDNL
                     RVGRHDGIASVIAALRGHPIFAHVTFTRSERSAVAALVTSLLDDPRV"
     gene            complement(1950..>2753)
                     /locus_tag="VNQ33_12240"
     CDS             complement(1950..>2753)
                     /locus_tag="VNQ33_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWJ98923.1"
                     /translation="YLADLAHVAAAAVWLGGLVAVTVVVRRRRRDDAPLDAAEAIARF
                     SGWAAVTAGVLIVAGTALSWIQVGGLDALTSTLYGRLLLAKVALVAVILAAAAWNRFR
                     LVPRVAAAAIEDPPVDDGASWKVLLRLVRLEAVLLVAVLMVTAVLANVTPAKESVDKG
                     PVTVSAPLGEGSVDVTVDPAKAGRNDIHIYLFDKDDRPDDDFQGAEVSLELPSQGLGP
                     FNRTTVDAGNGHYQLVATDLPLAGTWSMTVKVKVDRFTEQKATVTFPVS"
BASE COUNT          419 a         1032 c          936 g          366 t
ORIGIN      
        1 aacaggagca cggcccccgt gaggaagagc ccggccacca cgagctgcac gaggacgtcc
       61 ggcagcagcg acagggcgct tccggccgcg acggccacca cgacgtggat cgcgaacgcc
      121 gagaccgccc cgacccagac ccacagcggc cggcggtagc gggtcgtcag cacgatcgtg
      181 gcgatcatcg tcttgtccgg cagctcggcc gggaacacgg tgatgaaggc ctggagcagg
      241 gcgtggatcg acatgggggt tggttctccg atcgggcccc gaccggagct gcctccggcc
      301 gagaccacac agcagtggca tcagccgaag gtctcgcccg cctggcggac caggcccacc
      361 cgaccgggtg ccggagcacc agtgtgtcga tcgggcggtt gggggctact ccccttcgca
      421 cgaaccaggc taggcccgaa gatgtcggga ggatgaatcg ttgcccggca tccaccacct
      481 cgccgccgag cgggtgttgc attgctcctg gtgacccacc gcccagccgt cagcgactcc
      541 ggtccgtcgc aggaccctga agacgccgat ggctgccgac cgcccagggg gcttcgttga
      601 ccgcacttcc cacctctgtc ccgtcgcgtc ctcggaccta cgtgctcgac acctcggtgc
      661 tgctcgccga cccgcaggcg ctcgagcgct tcgacgagca cgccgtggtg ctgccgatcg
      721 tggtgctcat ggagctggaa gggaagcgca accaccccga gctgggctgg gcggcccgcc
      781 agagcctccg cacgctcgag cggctgcgct tggagcacgg atcgctcacg atcccgctcc
      841 cggtcaacgc ccggggcggc acgctgcgcg tcgagctgaa ccaccagtcc acggccggcc
      901 tgccacccac gatggcgtcg gacaacaacg accaccgcat cctcgccgtg gcccacaacc
      961 tgcggctgga gggccacgac gtcaccgtcg tcacgaagga cctcccgctg cggttgaagg
     1021 ccagcatcgt gggcctccac gccgacgagt accgcaacga gctggccgag gacacgtcgt
     1081 ggaccggctt cgtggagctg gacgtcgagc ccgacgtgat cgacgacctg ttcacccacc
     1141 gcgaggtcga tctgcccgag gccgccgagc tcccgtgcaa cacgggcgtc gccctccacg
     1201 ccggctcgca gtcggccctg gcccgggtcc acgccgacaa gtcgctccgc ctcatccgcg
     1261 gcgacctcag cctgttcgac gtcgtgggcc gcagcgccga gcagcgcatc gccctcgacg
     1321 tcctcgccga tccctcggtg agcatcgtga gcctgggcgg caacgccggg accggcaaga
     1381 gcgtgatggc cctggccgcc gggctcgagg ccgtcttgga gcgccgcacc cacaagcgga
     1441 tcttgatctt ccgcccgctc tacgccgtcg gcggccagga cctcgggttc ctgcccggcg
     1501 acaaggacga gaagatgggc ccgtgggccg gcgcggtgtt cgacgccctc gagtcgatcg
     1561 ccagccccga ggtggtcgag gaggtcatgg cccgcaacct cctcgaggtc ctgccgctga
     1621 cccacatccg cggccgcagc ctcaccgaca ccttcgtgat catcgacgag gcccagaacc
     1681 tggagcgccc ggtcctgctc accgcgctca gccgcctcgg cgagggcagc cgggtcgtgc
     1741 tcacccacga cgtcgcccag cgcgacaacc tccgggtggg tcgccacgac ggcatcgcct
     1801 cggtcatcgc cgcgctgcgg ggccacccga tcttcgccca cgtgaccttc acccggtccg
     1861 agcgctcggc ggtcgccgcc ctggtcacca gcctcctgga cgatcctcgg gtctgacctc
     1921 gggcccggcc gccggaccgc gccggccgtt caggagacgg ggaaggtgac cgtggccttc
     1981 tgctcggtga accggtcgac cttgaccttg accgtcatcg accacgtgcc cgcgagcggg
     2041 aggtcggtgg ccaccaactg gtagtggccg ttgccggcgt cgaccgtcgt gcggttgaac
     2101 gggcccaagc cctggctggg cagctcgagc gacacctcgg ctccctggaa gtcgtcgtca
     2161 gggcggtcgt ccttgtcgaa caggtagatg tggatgtcgt tgcgcccggc cttcgcggga
     2221 tcgacggtga cgtcgacgga gccctcgccg agcggggccg acacggtgac cggccccttg
     2281 tcgaccgatt ccttcgccgg cgtcacgttg gccagcacgg cggtgaccat cagcaccgcc
     2341 acgagcagga ccgcctcgag ccgcacgagg cgcagcagga ccttccacga ggccccgtcg
     2401 tcgaccggcg ggtcctcgat cgccgccgcc gcgacccggg gcacgagccg gaaccggttc
     2461 cacgccgcgg ccgccaagat cacggcgacc agggcgacct tggccagcag cagccggccg
     2521 tagagggtgg acgtcagggc gtcgagccct ccgacctgga tccagctgag ggcggtgccc
     2581 gccacgatga gcacgccagc ggtcaccgct gcccagcccg agaagcgggc gatcgcctcg
     2641 gcggcatcga gcggggcatc gtcgcgccgg cggcgccgga cgaccacggt caccgcgacc
     2701 aggccgccga gccacacggc ggcggcagcc acgtgggcga ggtcggccag gta
//
LOCUS       DATMFS010000639         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_275515, whole genome shotgun sequence.
ACCESSION   DATMFS010000639 DATMFS010000000
VERSION     DATMFS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275515"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VNQ33_12245"
     CDS             <1..451
                     /locus_tag="VNQ33_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWJ98924.1"
                     /translation="TWPSSTQGAEKSTGVIAVVKDTDGAIGYADLADAAKEGLTVAAI
                     GNTDGEFVAPTPDSASAALAAAEVADDLTYSPLNISAEGAYPITSPTWMLVDKVQSDQ
                     ATADILTAYLNYILTTGQEDAKGLLYAPLPDELAQKAIAQIDDITVG"
     gene            564..1550
                     /gene="pstC"
                     /locus_tag="VNQ33_12250"
     CDS             564..1550
                     /gene="pstC"
                     /locus_tag="VNQ33_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWJ98925.1"
                     /translation="MTAVNPEDVAASDSLVEGLDSTKGARADTVFRWAAFGAGLMVLV
                     ILALITFTTAQAAWPAFSVEYFTKAEWIPSQQQFGMLGFLWATLLISFIGILMAVPIA
                     VGIALFATEVAPRRLRRTITTVMDLLASIPSVVFGLVGFYVLRAPAQDLFRWIGDVCA
                     GIPVLDRIFGEGSSGTSILMAGIVLSIMILPIVTSISREVFDTVPRNDKEGALALGAT
                     RAEMINGVVIPHSLGGLTGAVMLGLGRAMGETIAVTLLIGGVSNPPITVDVLSASDAM
                     PSAIARYLSESAGTYRSALIGLGVTLFVITMVINVLSRKVVNVFDRRIKGAA"
     gene            1547..>2242
                     /locus_tag="VNQ33_12255"
     CDS             1547..>2242
                     /locus_tag="VNQ33_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ98926.1"
                     /translation="MTATQTPRPKVDERLVVPTDVRISTIKGARFRRNQIASIGMVAA
                     FVLAAIPLLFVVGNVVVKGLPVVTTKFLVSDIPNNAAAQELSSDCETLRRYDPTAACD
                     DKAPVEKPQPGMGPAVVGTLITTGLAALFAIPLGILAAIYLNEYGKQTKLARFIRFMT
                     DVMTGVPSIVMGIFIYQLWIVKFGNGRSALAASLSLACLMLPIVVRSSEEMLKLVPNT
                     LREASAGLGSRTWK"
BASE COUNT          342 a          832 c          709 g          359 t
ORIGIN      
        1 cacgtggccg tcgagcaccc agggcgccga gaagagcacc ggcgtcatcg ccgtggtcaa
       61 agacaccgac ggcgccatcg gctacgccga cctcgccgac gcggccaagg aaggcctgac
      121 cgtcgccgcc atcggcaaca ccgacggcga gttcgtcgcc ccgacgcccg acagcgcctc
      181 ggccgccctg gccgccgccg aggtcgctga cgacttgacc tacagccccc tcaacatcag
      241 cgccgagggt gcgtacccga tcacgtcgcc cacctggatg ctggtcgaca aggtgcagag
      301 cgaccaggcc accgccgaca tcttgacggc gtacctgaac tacatcctca ccaccgggca
      361 ggaggacgcc aagggcctcc tctacgcccc gctgcccgac gagctcgctc agaaggccat
      421 cgcccagatc gacgacatca ccgtcggctg atctgatccg tcgctgacgg gaccgggccg
      481 cctggcccgg tcccgtcgtg cgatcatcgc cgcccgcgac ctcacccgcg acgcccctgc
      541 ctcccctcca cccgaggaac gccttgaccg ctgtcaaccc cgaagacgtc gccgcgagcg
      601 actcgctcgt cgaaggcctc gactccacca agggcgccag agccgacacg gtgttccgct
      661 gggccgcctt cggcgccggc ttgatggtcc tcgtgatcct ggccctgatc accttcacca
      721 ccgcgcaggc ggcctggccc gccttcagcg tcgagtactt caccaaggcc gagtggatcc
      781 cgagccagca gcagttcggg atgctcgggt tcctgtgggc cacgctgctg atctccttca
      841 tcggcatcct catggcggtg ccgatcgccg tgggcatcgc cctgttcgcc accgaggtgg
      901 cgccgcggcg gctccggcgc acgatcacca cggtgatgga cctcctcgcc tcgatcccgt
      961 cggtcgtgtt cggcctcgtc ggcttctacg tcctgcgggc gccggcccag gacctgttcc
     1021 gctggatcgg cgacgtgtgc gccgggatcc cggtgctgga ccggatcttc ggcgagggct
     1081 cgtcgggcac cagcatcttg atggccggca tcgtgctgtc gatcatgatc ctgccgatcg
     1141 tcacctcgat ctcgcgggag gtgttcgaca ccgtcccccg caacgacaag gagggggcgc
     1201 tcgccctggg tgccacccgg gcggagatga tcaacggtgt cgtcatcccg cactcgctgg
     1261 gcggcctcac cggtgcggtg atgctcggcc tcggccgggc catgggcgag accatcgccg
     1321 tgaccctgct catcggcggg gtgtccaacc cgccgatcac cgtcgacgtc ttgagcgcca
     1381 gtgacgccat gccgtcggcg atcgcccgct acctctccga gagcgccggc acgtaccgat
     1441 cggcgctcat cggcctcggc gtcaccctct tcgtgatcac catggtgatc aacgtcttgt
     1501 cccgcaaggt ggtcaacgtg ttcgaccggc gcatcaaggg ggcggcgtga ccgcgaccca
     1561 gacgccccgc cccaaggtgg acgagcgcct cgtcgtgccc acggatgtgc gcatcagcac
     1621 catcaagggg gcccgcttcc gccgcaacca gatcgcgtcg atcgggatgg tcgccgcctt
     1681 cgtgctcgcc gccatcccgc tgctgttcgt cgtcggcaac gtcgtggtga agggcctgcc
     1741 cgtcgtcacg acgaagttcc tggtgagcga catccccaac aacgccgccg cgcaggagct
     1801 gagcagcgac tgcgagacgc tgcgcaggta cgaccccacc gcggcgtgcg acgacaaggc
     1861 gccggtcgaa aagccccagc ccggcatggg cccggcggtg gtcgggacgc tcatcaccac
     1921 gggcctggcg gcgctgttcg ccatccccct cgggatcctc gccgccatct acctcaacga
     1981 gtacgggaag cagacgaagc tcgcccgctt catccggttc atgaccgatg tcatgacggg
     2041 cgttccgtcc atcgtgatgg gcatcttcat ctaccagctg tggatcgtga agttcggcaa
     2101 cgggcgctcg gcgctggctg cgtcgctgtc gctggcgtgc ctgatgctcc cgatcgtggt
     2161 gcggtccagc gaagagatgc tgaagctggt ccccaacacc ctgcgtgaag ccagcgccgg
     2221 gctcgggtcg cggacctgga ag
//
LOCUS       DATMFS010000640         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_252500, whole genome shotgun sequence.
ACCESSION   DATMFS010000640 DATMFS010000000
VERSION     DATMFS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252500"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /gene="def"
                     /locus_tag="VNQ33_12260"
     CDS             complement(<1..501)
                     /gene="def"
                     /locus_tag="VNQ33_12260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWJ98927.1"
                     /translation="MAPYEIRVIGDPVLKQKAVDVTDIDGKLVRLVDDMVDTMYAAVG
                     LGVAAPQVGVQKRLFVWDLQDGDGPKSIINPEIIESDGEWVYEEGCLSVPGLSWDIVR
                     PNRVHVVGRDLDGNEVSIEASELEGRMFQHELDHLNGVLLIERLDEDQARAAKRQVRD
                     LMLGFGA"
     gene            526..1158
                     /locus_tag="VNQ33_12265"
     CDS             526..1158
                     /locus_tag="VNQ33_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWJ98928.1"
                     /translation="MPSPQPERADHYFSSDPAAPSAPQTIELVLPEGRLIPLTTDRGT
                     FSPDRVDDGTRALLAEGPAVPPAGVLADVGCGYGPIAVTLALRGGPGIEVWAVDVNER
                     ARALCQANAEANGVADRVRVAAPENVPADLVVDQMWSNPPIRIGKPALHELLATWLDR
                     LSPGTGTAELVVQKHLGADSLARWLGDQGWTAERVASRGGYRILHVAVRS"
     gene            1155..1685
                     /locus_tag="VNQ33_12270"
     CDS             1155..1685
                     /locus_tag="VNQ33_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWJ98929.1"
                     /translation="MSRNLGSTDLKRLHREWRRRAPGRIGLLLDSVATPANVGSILRT
                     AAALRIDDVWVCGQTAGPEVGGTKKTSLGSERYLTFHEVEQPAAAVVAAQVAGYRVIG
                     IELAEAAVPIHAADLTGSVCLALGHEDRGLTPAVLQGADQLAFIPQLGRIGSLNVATA
                     ASIAMYETRRQGWPSV"
     gene            complement(1769..>2894)
                     /locus_tag="VNQ33_12275"
     CDS             complement(1769..>2894)
                     /locus_tag="VNQ33_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98930.1"
                     /translation="VVLDEHDEAYQQEQAPTWHARDVAIERARRAGVPCLLVSPCPSL
                     EALQWGDLTLDDRSAERAAWPRIDLVDQRDLDPALGPLFSPRLVDVLRSDRSVVCVLN
                     RTGRARLLACTACSSLARCAVCDAAVHQTEAAAGTEGFGCGRCGTERPMVCLECGGHR
                     FKNLRLGVSRAREELEALAGTTVAELTATTPEDDPAVADARIVVGTEAALHRVRQAEV
                     VAFLDFDQELLALRYRAAEEAMALLVRAGRLVQRAAVRRGADGPDRRGLVLVQTRTPD
                     HPVLAAAHQADPGRLVASELPMRQALGLPPTTAMAAVSGAAAPELMGALGEPEGIRVQ
                     GPLGDAWRIIAPDHETLTAALRSVPRPSGRLRIAVDPLRL"
BASE COUNT          392 a         1114 c         1005 g          383 t
ORIGIN      
        1 cgcgccgaac ccgagcatga ggtcgcgcac ctggcgcttg gccgcacgag cctggtcctc
       61 gtcgaggcgc tcgatgagca agacgccgtt caggtggtcc agctcgtgtt ggaacatgcg
      121 cccttcgagc tccgatgcct cgatcgagac ctcgttcccg tcgaggtcgc ggccgaccac
      181 gtgcacacgg ttgggccgca cgatgtccca cgagaggccc ggcaccgaca ggcacccctc
      241 ctcgtagacc cactcgccgt cggactcgat gatctcgggg ttgatgatgc tcttcggtcc
      301 gtcaccgtcc tggaggtccc acacgaacag gcgcttctgc acgcccacct gcggggccgc
      361 cacgccgagg cccacggcgg cgtacatggt gtccaccatg tcgtcgacga ggcgcacgag
      421 cttgccgtcg atgtcggtca cgtcgacggc cttctgcttc aggacgggat cgccgatcac
      481 gcggatctcg tagggcgcca tgggagccag gctaccgtcg ccggggtgcc gtccccccag
      541 cccgagcggg ccgaccacta cttctcctcg gatcccgccg ccccctcggc gccccagacc
      601 atcgagctgg tgctgccaga aggacgtctg atcccgctca cgacggaccg ggggacgttc
      661 tcgcccgatc gggtcgacga cggcacccgc gccctgctgg cggagggccc agcggtcccc
      721 ccggccggcg tgctggccga cgtcgggtgc gggtacggac ccatcgccgt gacgctggcg
      781 ctgcgcggcg gccctgggat cgaggtctgg gcggtcgacg tgaacgagcg cgctcgggcc
      841 ctgtgccagg cgaacgccga ggccaacggt gtggcggacc gggtgcgcgt cgccgccccg
      901 gagaacgtgc cggccgacct ggtcgtcgac cagatgtggt ccaacccgcc gatccgcatc
      961 ggcaagcccg ccctccacga gctcctcgcc acgtggctcg accgcctctc acccggcacc
     1021 ggcacggccg agctggtggt ccagaagcac ctcggggccg actcgctcgc ccggtggctg
     1081 ggagaccagg ggtggaccgc cgagcgcgtc gcctctcgtg gcggctaccg gatcctgcac
     1141 gtggcggtcc gttcgtgagc cgcaacctgg gcagcaccga tctcaagcgc ctccaccgcg
     1201 agtggcggcg ccgggcaccg gggcgcatcg gcctgctcct ggactcggtg gccaccccgg
     1261 ccaacgtcgg ttcgatcctg cgcacggccg ccgccctgcg catcgacgac gtgtgggtct
     1321 gcggccagac cgccggcccg gaggtgggcg gcaccaagaa gacctcgctc ggctccgagc
     1381 gctacctcac gttccacgag gtcgaacaac ccgctgccgc cgtggtggcg gcccaggtcg
     1441 ccggctaccg ggtgatcggc atcgagctgg ccgaggcggc ggtcccgatc catgcggccg
     1501 acctcaccgg atcggtgtgc ctcgcgctcg gccacgagga ccgcggcctc accccggcgg
     1561 tgctccaagg cgccgaccag ctggcgttca tcccccagct cggccgcatc ggctcgctga
     1621 acgtggccac cgccgcgtcc atcgccatgt acgagacccg ccgccaaggc tggccgtccg
     1681 tctagaccga cctcccccgc ccctgccagc cgaagttggt gtgggaaacg aggtagagcg
     1741 tcgtttccca caccaacttc ggctcgtgct acagccggag cgggtcgacg gcgatccgga
     1801 gtcggcccga cggccgcggc acggaccgca gggcggcggt cagcgtctcg tgatcggggg
     1861 cgatgatgcg ccacgcgtcg cccaacgggc cctgcacccg gatgccctcc ggctcgccga
     1921 gcgcgcccat gagctcgggg gccgcggcac ccgagaccgc ggccatcgcg gtcgtcggcg
     1981 gcagcccgag cgcctggcgc atcggaagct cgctggccac cagccgtccg ggatcggcct
     2041 ggtgggcggc agccaagacc ggatggtccg gcgtgcgggt ctggaccagc accagcccgc
     2101 ggcggtcggg tccgtcggcc ccgcggcgca cggcggcgcg ctgcacgagc cgacccgctc
     2161 gcaccagcag ggccatcgcc tcctcggcgg cgcggtagcg cagggccagc agttcctggt
     2221 cgaagtcgag gaacgccacc acctccgcct gccggacacg gtgcaaggcc gcttcggtgc
     2281 cgaccacgat ccgagcgtcc gccacggcgg ggtcgtcctc gggcgtcgtc gccgtcagtt
     2341 cggcgacggt cgtcccggcg agcgcctcga gctcctcccg ggcccggctc acgccgagcc
     2401 gaaggttctt gaagcggtga ccgccgcact ccaggcacac catcggccgc tcggtcccgc
     2461 agcgtccgca cccaaacccc tcggtcccgg cagcggcctc ggtctggtgc accgcggcgt
     2521 cgcacaccgc gcaccgcgcc agcgacgagc acgccgtgca cgccagcagg cgggcgcggc
     2581 ccgtccggtt cagcacgcac accaccgatc ggtcgctgcg gagcacgtcg accaaccggg
     2641 gcgagaagag cggcccgagc gcggggtcca ggtcccgttg gtcgacgagg tcgatgcgtg
     2701 gccacgccgc acgctcggcc gaccggtcgt cgagcgtcag gtcgccccac tgcagcgctt
     2761 cgagcgacgg gcacggcgac acgagcaggc agggcacacc cgcccgccgg gctcgctcga
     2821 tcgccacgtc gcgggcgtgc caggtcggcg cctgctcctg ctggtaggcc tcgtcgtgct
     2881 cgtccagcac cacg
//
LOCUS       DATMFS010000641         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_21974, whole genome shotgun sequence.
ACCESSION   DATMFS010000641 DATMFS010000000
VERSION     DATMFS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21974"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VNQ33_12280"
     CDS             complement(<1..679)
                     /locus_tag="VNQ33_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98931.1"
                     /translation="MTASFLGEDYLEIRNLTVDFDGFKAVDGVDLTFHQGRLHFLIGP
                     NGAGKTTVVDALTGLVSGTGRAEFRGRDVLTMKSHRIVRLGIGRTFQTATVFEQLTVL
                     QNLDIAAGLHRRAGRLLLARGKVPDRVVEALETIGLTALADRPAGFLAHGQRQWLEVG
                     MLLVQDASVMFLDETVAGMSAEEREETGDLLKRIMADRTIVVIEHDMEFMRRYADFVT
                     VLHAGAVL"
     gene            complement(676..1881)
                     /gene="urtC"
                     /locus_tag="VNQ33_12285"
     CDS             complement(676..1881)
                     /gene="urtC"
                     /locus_tag="VNQ33_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWJ98932.1"
                     /translation="MTAADPRSPDAAEAEPAAAPAASSALGSRFTPAKLLSAYGPLLA
                     IGVLALVMLVWAPGALSPFRLGNLGKYCAWALAAIGIGLAWGRGGMLVMGQGVFFGLG
                     GYAMAMHMKLEAAGPGGVPDFMTLYGNGTMPGWWEPFRSGPFTLFMIVAAPAVISFIL
                     GYGILKRRVKGAYFAILTQALAVAFSTLLISTIKQTGGFNGLNTFSTFFGENLYDPDV
                     KKKVYLITAGLMIGCLVIVWQLYRSRYGELLIATRDQEERVRFLGYDPANVKLVAFVA
                     AAVMASIGGAMFVPIAGIISPSSVDAAASIMLLAGVALGGRSSLLGPALGAIAVGFGQ
                     SSLSERFPTQWTYFQGALFIIVMLFLPAGVASIWPRLKALLPARSTTAGPTTATTDAD
                     VDAGAVAPA"
     gene            complement(1878..>2305)
                     /locus_tag="VNQ33_12290"
     CDS             complement(1878..>2305)
                     /locus_tag="VNQ33_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98933.1"
                     /translation="AVGALSAVLKYSSFGRRIRATVQNRELAETLGVSTRSVDRITFF
                     LGSGLAGVGGVAIALISGTNPTLGTTYIIPAFLVVVAGGVGQIKGTVIAAFAVGIVTA
                     YLTDWTSGSMSQVIVFALVVVFLQVRPQGLFTVRTRSLA"
BASE COUNT          382 a          788 c          831 g          304 t
ORIGIN      
        1 cgagcacggc gcccgcgtgc aagacggtca cgaagtcggc gtagcgccgc atgaactcca
       61 tgtcgtgctc gatcacgacg atggtgcggt ccgccatgat ccgcttgagc aggtcccccg
      121 tctcctcgcg ctcctcggcg ctcatcccgg cgacggtctc gtccaggaac atcacgctgg
      181 cgtcttgcac cagcagcatc ccgacctcga gccactgccg ctggccgtgg gcgaggaagc
      241 cggccgggcg gtcggccagc gcggtgaggc cgatggtctc cagcgcctcg acgacccggt
      301 cggggacctt gccccgggcc agcagcaggc gccccgcccg gcggtggagc cccgccgcga
      361 tgtcgaggtt ctgcaagacg gtgagctgct cgaacaccgt ggcggtctgg aaggtccggc
      421 cgatgcccag gcgcacgatc ctgtgcgact tcatggtgag cacgtccctg ccgcggaact
      481 cggctcggcc ggtgccgctc accagccccg tcagcgcgtc gaccacggtg gtcttccccg
      541 cgccgttcgg cccgatcagg aagtgcagcc gaccttggtg gaacgtgagg tcgacgccgt
      601 cgacggcctt gaacccgtcg aagtcgaccg tgaggttgcg gatctcgagg tagtcctctc
      661 cgaggaacga ggcggtcatg ccggcgccac cgccccggcg tcgacgtcgg catcggtcgt
      721 ggcggtggtg gggccggcgg tcgtcgacct cgccgggagc agggccttga gccgcggcca
      781 gatcgaggcg acgccagcgg gcaggaagag catgacgatg atgaacagcg cgccctggaa
      841 gtacgtccac tgcgtgggga accgctccga gagcgacgac tggccgaagc cgacggcgat
      901 ggcgccgagc gccggaccga gcagcgagga gcgtcctccg agggcgacgc cggcgagcag
      961 catgatcgac gcggcggcgt ccacgctcga cggcgagatg atgccggcga tcgggacgaa
     1021 catggccccg ccgatgctgg ccatgacggc ggccgccacg aacgccacca gcttgacgtt
     1081 ggcggggtcg tagccgagga accgcacccg ctcctcctgg tcccgggtgg cgatcaggag
     1141 ctcgccgtag cggctgcggt acagctgcca cacgatgacg aggcacccga tcatcaagcc
     1201 ggccgtgatc aggtacacct tcttcttcac gtccgggtcg tagaggttct cgccgaagaa
     1261 ggtcgagaag gtgttgaggc cgttgaaccc gcccgtctgc ttgatcgtcg agatcagcag
     1321 cgtcgagaag gcgacggcca gcgcctgggt gaggatggcg aagtacgccc ccttcacccg
     1381 ccgcttgagg atcccgtacc cgaggatgaa cgagatcacc gccggcgcgg cgacgatcat
     1441 gaacaaggtg aacggcccgc tgcggaacgg ctcccaccag cccggcatgg tgccgttgcc
     1501 gtagagggtc atgaagtcgg gcacgccgcc ggggccggcg gcctcgagct tcatgtgcat
     1561 ggccatggcg tagccgccga ggccgaagaa cacgccctgg cccatgacga gcatcccgcc
     1621 acggccccat gcgaggccga tgccgatggc ggccagcgcc caggcgcagt acttgccgag
     1681 gttgccgagc cggaacgggc tcagcgcgcc gggagcccac acgagcatga cgagggccag
     1741 cacgccgatg gccaggaggg gtccgtaggc ggagagcagc ttggccgggg tgaaccggct
     1801 cccgagcgcc gaggaggccg ccggtgccgc ggcgggctcg gcctcggcag cgtccggcga
     1861 ccggggatcg gcggccgtca tgcgagcgac ctcgtccgca cggtgaacag cccctgcggg
     1921 cgcacctgga ggaagaccac gacgagggcg aacacgatga cctgcgacat gctgccgctg
     1981 gtccagtcgg tcaggtaggc cgtgacgatg ccgacggcga aggcggcgat caccgtgccc
     2041 ttgatctggc ccacaccgcc ggcgacgacc acgaggaacg ccgggatgat gtaggtggtc
     2101 ccgagcgtgg ggttggtccc cgagatcaag gcgatggcga caccgccgac ccccgccagg
     2161 cccgagccca ggaagaacgt gatccggtcc acgctgcggg tcgacacccc gagcgtctcg
     2221 gccagctccc ggttctgcac cgtggctcgg atgcggcggc cgaacgagct gtacttcagc
     2281 acggcgctga gggcgcccac cgcgg
//
LOCUS       DATMFS010000642         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_67773, whole genome shotgun sequence.
ACCESSION   DATMFS010000642 DATMFS010000000
VERSION     DATMFS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67773"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..150
                     /locus_tag="VNQ33_12295"
     CDS             10..150
                     /locus_tag="VNQ33_12295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWJ98934.1"
                     /translation="MTECLGGAEAILDDHLGQRYETMCDPRLNGRQSVDLAFAVAELL
                     QR"
     gene            190..1497
                     /locus_tag="VNQ33_12300"
     CDS             190..1497
                     /locus_tag="VNQ33_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98935.1"
                     /translation="MGERMRRRLDRDRLALWVIAAGSLVATVGWRGMSKPMADTVGYR
                     ATVFSLETGWHEANDRLIGYPLVLMATGSGHHSTHLLFFLQLAMHAVTVLMVVDLARR
                     FGLGRWGGAAVAALLYAPVVLVRVVQEGTEGLAALLLTLVFWLVLTPPRPGHRIRWAL
                     VLGLVCGASALVRPTFTLLFIPVMVLAARSVPARRWRTAGLVALPALVLVGGWIVVNG
                     VRFDLWSTTPNTAFNLNSRTSTYVERLPPRFEPGRSVLVEERDEALLLGETFPPQNYI
                     YRARPALEEATGLKDRALERYVMEVDLYLILHHPFNYLEAVKVSALNYSSIDGSPAVG
                     DFGRSIAWAQTGIHQFLLVAFFGVFALVPGLALAGRVERQHLRLFAVGFALAGYTFLV
                     TVLVEAGVARLRAPTEPILALMLVLGASIVRQAWRDRRSVAAE"
     gene            1648..>2681
                     /locus_tag="VNQ33_12305"
     CDS             1648..>2681
                     /locus_tag="VNQ33_12305"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWJ98936.1"
                     /translation="MLQEREIDPGQAGDIDKFERILAGYFDGTISEEVFRVFRLTNGI
                     YGQRQGGHNQMVRVRIPYGGVTPEQLDTLGHIADEYSRGWGHITTRQNVQLHYVPLER
                     VPEVMRVLGRVGLTTREACGDAVRNVMGCHLAGACPQEHLDITQWAEAANRHFLRNPI
                     AQRMPRKFKINFSGCETDCGQAMFNDIGVVASRREREDGSVELGFRVFVAGGLGANPF
                     PALLLEDFTAREDLLHTLESILRVFHQTGNRDNKLRARMKWVVQTLGWEETQRRILKE
                     RKFLLASNSWPGGIPAEVEKYGDEPAGVHATVTPTPVGQGTPVTLRSNVPYERWAEGN
                     VIRGAAKGTVS"
BASE COUNT          370 a          992 c          896 g          423 t
ORIGIN      
        1 ggcgatgacg tgaccgagtg cctgggcggc gccgaggcca tcctcgacga ccacctcggc
       61 cagcgctacg agacgatgtg cgacccgcgg ctgaacggtc gccagtccgt cgacctcgcg
      121 ttcgcggtcg ccgagctcct ccagcggtag gacctcgacg gctcggccag cctcctaggc
      181 tcccccgccg tgggagaacg gatgcggcga cggctcgacc gggaccggct ggcgctgtgg
      241 gtgatcgcgg ccggctcgct ggtcgccacg gtcggctggc gcgggatgtc gaagccgatg
      301 gccgataccg tcggctaccg ggccacggtg ttcagcctcg agaccggctg gcacgaggcc
      361 aacgaccgcc tgatcggcta cccgctggtg ctgatggcga cggggtccgg gcaccactcg
      421 acgcacctcc tgttcttcct ccaactggcg atgcacgccg tgaccgtgct catggtggtc
      481 gacctcgctc gacggttcgg cctcgggcgg tggggcggtg ccgccgtcgc cgccctgctc
      541 tacgccccgg tcgtgttggt gcgcgtggtg caggagggaa cggaggggct cgccgcgctc
      601 ctcctcaccc tcgtcttctg gctggtgctc acccctcccc gtcccgggca ccggatccgg
      661 tgggccctcg tgctcgggct ggtgtgcggc gccagcgcgc tcgtccggcc gacgttcacg
      721 ctgctcttca tcccggtgat ggtcctcgcg gcccggtcgg ttccggcgcg acggtggcgg
      781 accgccgggc tcgtcgccct ccctgcgctg gtgctggtcg gcggatggat cgtggtcaac
      841 ggggtgcgct tcgacctctg gagcacgacc ccgaacaccg ccttcaacct caactcgcgc
      901 acgagcacct acgtcgagcg gctgccaccg aggttcgagc ccggccgatc ggtcctggtc
      961 gaggagcggg acgaggcact gctgctcggg gagacgttcc caccgcagaa ctacatctac
     1021 cgggcccgcc cggccctcga ggaggccacg ggcctgaagg accgggcgct cgagcggtac
     1081 gtcatggagg tggacctcta cctgatcctc caccacccgt tcaactacct cgaagccgtg
     1141 aaggtgtcgg cgctcaacta ctcgagcatc gacggatccc ccgccgtcgg cgacttcggc
     1201 cggtcgatcg cctgggcgca gacggggatc caccagttcc tcctggtcgc cttcttcggc
     1261 gtcttcgccc tcgttcccgg actggccctc gccggccgcg tcgagcggca gcacctgcgc
     1321 ctgttcgcgg tcggcttcgc ccttgccggc tacacgttcc tcgtcaccgt cctggtcgag
     1381 gccggcgtgg cccggctgcg cgccccgacc gagccgatcc tcgccctgat gctcgtgctc
     1441 ggcgcctcca tcgtgcgcca ggcctggcgg gaccgccgct ccgtcgctgc cgaatgaccg
     1501 cggttcgggg gcatttgccc tggccagggc gtttgcgtgc gtgacccttg gctcaattgt
     1561 tgacccgact gctcaagatt tgagacgctg gagggaattc gtgacctgtc tggtcggtta
     1621 tccgacaggc actcaaggga gccagccatg ttgcaagagc gcgagatcga cccgggccag
     1681 gccggagaca tcgacaagtt cgagcggatc ctcgccgggt acttcgacgg caccatctcc
     1741 gaggaggtgt tccgcgtctt ccgcctgacg aacggcatct acggccagcg ccagggcggc
     1801 cacaaccaga tggtgcgggt ccgcatcccc tacggcgggg tcacacccga gcagctcgac
     1861 acgctcggcc acatcgccga cgagtactcg cggggctggg gccacatcac cacccgccag
     1921 aacgtgcagc tccactacgt gccgctggag cgggtgcccg aggtcatgcg cgtgctcggc
     1981 cgggtcgggc tcaccacccg tgaggcctgc ggcgacgccg tgcgcaacgt catgggctgc
     2041 cacctcgccg gcgcctgccc ccaggagcac ctcgacatca cgcagtgggc cgaggccgcc
     2101 aaccgccact tcctgcgcaa ccccatcgcc cagcggatgc cccgcaagtt caagatcaac
     2161 ttctcgggct gcgagaccga ctgcggccag gccatgttca acgacatcgg cgtcgtcgcc
     2221 agccgccgcg agcgcgagga cggctcggtg gagctcgggt tccgggtctt cgtcgcgggc
     2281 ggcctcggcg ccaacccctt ccccgccctc ctgctcgagg acttcacggc ccgcgaggac
     2341 ctgctccaca ccctcgagtc gatcctgcgg gtgttccacc agacgggcaa ccgcgacaac
     2401 aagctccgcg cccgcatgaa gtgggtcgtc cagaccctcg gctgggagga gacgcagcgg
     2461 cgcatcctca aggagcgcaa gttcctgctg gcctccaaca gctggcccgg cggcatcccg
     2521 gcggaggtcg agaagtacgg cgacgagccc gctggcgtcc acgccaccgt cacgcccacg
     2581 ccggtcggcc agggcacgcc ggtcacgctc cggtccaacg tcccctacga gcgctgggcc
     2641 gagggcaacg tgatccgcgg cgccgccaag ggcacggtgt c
//
LOCUS       DATMFS010000643         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_275597, whole genome shotgun sequence.
ACCESSION   DATMFS010000643 DATMFS010000000
VERSION     DATMFS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275597"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="tmk"
                     /locus_tag="VNQ33_12310"
     CDS             <1..408
                     /gene="tmk"
                     /locus_tag="VNQ33_12310"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWJ98937.1"
                     /translation="HVATKIRPALNRGKDVVCDRFVGSSLAYQGYARDLGIEEVRALS
                     TFATDGLVPDLVLLMVVPPEEAAIRLAAAGTPDRLEAAGEEFHRKVAEAYLILAAHDP
                     DRWVVIDGSGTVGEVGARVLEAVEARLPSEAGT"
     gene            405..1490
                     /locus_tag="VNQ33_12315"
     CDS             405..1490
                     /locus_tag="VNQ33_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98938.1"
                     /translation="MSLPGLDAPPEDPWSDVVGQQAAVAMLRHAAQGDTPHAWLFVGP
                     HGSGKRAAARAFAGDLLAAEAIEAGDDERASRARALARAEQHPDLVIVDREGASISVG
                     QADEIIRRASRSAIEGSRKVLLLDEFHLVKDAGPKLLKTIEEPPPGTFFVVLADELPP
                     ELITIASRCVRVDFDALTAPLIAARLVDEGVAPERAGEVAAFAGGDLDRARLLATDER
                     LALRLAAWRDLPRRLNGSGSAAAAGIADLRAAIDDAEAPLKARHEVEVAELNERIERY
                     GQRGSGARQLDERHRRESRRLRTDELRMGLGALAAAYRDEMAVAADPSRAIAALDAIQ
                     ETAERLEFNPNEELQLIALAIRLPTLT"
     gene            complement(1483..2031)
                     /locus_tag="VNQ33_12320"
     CDS             complement(1483..2031)
                     /locus_tag="VNQ33_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98939.1"
                     /translation="MTGLGSKVVGLHLALDGATIPHAFGGALALAFCTLRPRGTSDID
                     LNVFLSVAERDRFLTSLPREVTPTEEQRMLLERDGQARLFWDRTPIDVFLDTTHFHRG
                     ARLRIQWQEFGGHLMPFLGCSDLAVFKAFFDRTKDWADLEEMIAAGSLDRERVLGALV
                     LYLGGSDPRIERLRTLGPPGTR"
     gene            complement(2028..2342)
                     /locus_tag="VNQ33_12325"
     CDS             complement(2028..2342)
                     /locus_tag="VNQ33_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ98940.1"
                     /translation="MQPASILRACRQQRGLTLRDLSRRAGTSHSTLAAYESGRKVPGA
                     DTLLRVVRAAGFDLIVGPYAFEVSSDPRNRGEELEEVLALADMLPSRHARTLDAPIFE
                     RG"
BASE COUNT          370 a          889 c          898 g          344 t
ORIGIN      
        1 cacgtggcca cgaagatccg cccggcgctg aaccggggca aggacgtcgt gtgcgaccgg
       61 ttcgtcgggt cctcgctggc gtaccagggc tacgcccgtg acctgggcat cgaggaggtg
      121 cgggcgctgt cgacgttcgc caccgacggg ctcgtgcccg acctcgtgct cttgatggtc
      181 gtgccgcccg aggaggccgc catccggttg gccgccgccg gcacacccga ccggctggag
      241 gccgcgggcg aggagttcca ccgcaaggtc gccgaggcgt acctcatctt ggccgcccac
      301 gacccggacc ggtgggtggt gatcgacgga tcgggcaccg tcggcgaggt cggggcgcgg
      361 gtgctcgaag cggtcgaggc gcggctgccg tcggaggcgg gcacatgagc ctgccgggcc
      421 tcgacgcgcc gccggaggac ccgtggtccg acgtcgtcgg ccagcaggcc gccgtcgcca
      481 tgctccgtca cgccgcccag ggcgacacgc cccacgcctg gctgttcgtc gggccgcacg
      541 gctccggcaa gcgggctgcc gctcgggcct tcgccggcga tctgctggca gccgaggcca
      601 tcgaggccgg cgacgacgag cgcgcgtccc gagcccgggc gctggctcgg gcggagcagc
      661 accccgacct ggtgatcgtc gaccgcgagg gcgccagcat ctcggtgggc caggccgacg
      721 agatcatccg ccgcgccagc cggtccgcca tcgaggggtc gcgcaaggtc ttgctgctcg
      781 acgagttcca cctggtgaaa gacgccggcc cgaagctgct caagaccatc gaggagccgc
      841 cgcccggcac gttcttcgtg gtgctcgccg acgagctgcc gcccgagctc atcaccatcg
      901 ccagccggtg cgtgcgggtc gacttcgatg ccctcaccgc cccgctcatc gccgcccgcc
      961 tggtcgacga gggcgtggcg cccgagcggg ctggcgaggt ggccgccttc gccggcggcg
     1021 acttggaccg ggcccggctc ctcgccaccg acgagcgcct cgccctccgg ttggccgctt
     1081 ggcgagacct gccgcgccgg ctgaacggct cgggcagcgc ggccgcggcc ggcatcgccg
     1141 acctgcgcgc cgccatcgac gatgccgagg cgccgctcaa ggcgcgtcac gaagtcgagg
     1201 tggccgagct gaacgagcgg atcgagcgct atggccagcg gggttcgggc gcgcgccagc
     1261 tagacgagcg ccatcggcgg gagagccgcc gcctccgcac cgacgagctg cgcatgggcc
     1321 tcggcgctct cgccgcggcc taccgcgatg agatggccgt ggctgctgac ccgtcccgtg
     1381 cgatcgccgc gctggacgcc atccaggaga cggctgagcg tctcgagttc aatcccaacg
     1441 aggagctcca gctcatcgcc ctcgccatcc gcctccccac cctcacctag tgcccggagg
     1501 gccgagggtg cgcagccgtt cgatccgggg atccgacccg ccgaggtaga gcaccagcgc
     1561 gccgaggact cgctcgcggt cgagcgaacc cgctgcgatc atctcctcca gatcggccca
     1621 gtccttggtg cggtcgaaga aggccttgaa gaccgcaagg tccgagcagc cgaggaacgg
     1681 catgaggtgc ccgccgaact cctgccactg gatccggagc ctcgcaccac gatggaagtg
     1741 cgtcgtgtcc aagaagacgt cgatcggggt gcggtcccag aacaagcgcg cctgaccgtc
     1801 gcgctcgagg agcatccgct gctcctcggt gggcgtgacc tcacgcggca acgaggtcag
     1861 gaaccggtcg cgctcggcga cgctcaggaa cacgttgaga tcgatgtcgc tggtgcctcg
     1921 cgggcggagg gtgcagaagg cgagcgcgag cgcccctccg aacgcatggg ggatcgttgc
     1981 gccgtcgagc gcgaggtgga ggccgaccac cttggatccg agtccggtca tccgcgttcg
     2041 aagatcggag cgtcgagggt tcgggcgtgg cggctcggga gcatgtcggc gagggcaagc
     2101 acctcctcga gctcctcgcc ccggttccgc ggatcgctgc tgacctcgaa ggcgtagggc
     2161 ccgacgatga ggtcgaagcc ggcagcccgc acgactcgga gcagcgtgtc ggctcccggg
     2221 accttccggc cggactcgta cgcggccagg gtcgagtggg aggtgccggc ccgacggctg
     2281 aggtcacgca acgtcagtcc ccgttgctgc cggcacgctc gcaggatcga agcgggttgc
     2341 atgatcagat ggtatccgac cagataccag cgaggacggc cgcagtttcc aaaccagacg
     2401 accgaacggg cttgccgtcg cccctcagcg ttcgggtacg gttgcccctc cacgcccaag
     2461 tagctcagtc ggcagagcat ctcactcgta atgagaaggt c
//
LOCUS       DATMFS010000644         3788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_348127, whole genome shotgun sequence.
ACCESSION   DATMFS010000644 DATMFS010000000
VERSION     DATMFS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348127"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="VNQ33_12330"
     CDS             complement(<1..2367)
                     /locus_tag="VNQ33_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWJ98941.1"
                     /translation="MRTPPPAQGLYDPRFEHENCGVAFVVDMHGRRTHQLVEQGITAL
                     VHLDHRGASGAETNTGDGAGILIQVPDALYRAELGVELPAAGAYATGIGFFPGDDAEV
                     TSAVEAVGKIAASEGLEVLAWRDVPVDASSLGSGALDAMPAFRQVFLAAPGGTVTDLD
                     LERRVYVVRKRIEHEIGAGDRRIYFPSLSARTIVYKGMLTTPQLRDFYPDLADERVES
                     AIALVHSRFSTNTFPSWPLAHPYRYLAHNGEINTVEGNRNWMRAREALLASDLFPGDI
                     SRLFPICTPGASDTASFDEVLELLHLGGRPLPHAVLMMIPEAWENHRHMDPARKAFYR
                     FHASLMEPWDGPANVSFTDGTVIGAVLDRNGLRPGRFWVTADGLVVLGSEAGVLDIPA
                     DQIVRKGRLQPGKMFLVDTAQGRIIEDDEVKAGLAAELPYQAWLDQGIIELDELPERP
                     HVRYPHGDVVRRQLTFGYTIEDQKILIAPMARTGGEALGSMGTDTPIAVLSERPRLLF
                     DYFAQRFAQVTNPPLDAIREEMVTSLGGTIGPEQNLLAPGPRSCRQIQLPFPILDNDQ
                     LAKLIHVEDSVDDFRAVVVQGLYPVAGGGDALREAIESVRRQVSDAIEGGANVVILSD
                     RDSTRELAPIPSLLLTAAVHHHLVREKTRTAVGLVVEAGDAREVHHMALLIGYGAAAI
                     NPYLAFETIEDMAARGALPDVGLDKAVKNYIKGAGKGVLKVMSKMGISTVASYTGAQV
                     FEAVGLGQGLIDEYFTGTTSKLGGIGLDEVATEVALRHAKAYPDRPTER"
     gene            2520..>3788
                     /locus_tag="VNQ33_12335"
     CDS             2520..>3788
                     /locus_tag="VNQ33_12335"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HWJ98942.1"
                     /translation="MSDAQDLLAFIGAAPSPYHAVGEARRRLADAGFTALDLTEPWPA
                     EPGRYLVTRGGALVAWEVPDDAGPAQRFRIVGAHTDSPNLRIKPRPDTGSAGWQQLGV
                     EVYGGALWNTWLDRDLGLSGRAVVRVDGAVEERLFHCDCPLLRIPQLAIHLDRDVNGQ
                     GLKLNPQQHLTPVWGVGPVEPGAFAAYVAGEVAVAPADVVAWDAMVHDLTPGATIGRR
                     HELVSTARLDNLLSCWAGVTAIAEPGSGSGRPVSVLCLFDHEEVGSTSSTGADGALLA
                     QVLERTVSARGGSRDDLLRALAGSVCASADGAHATHPNYPERHDPGHLVAIDGGPVVK
                     VNANQRYATDAAGHAVLIDAADRAGVPHQVFVSRNDQPCGSTIGPMTAARLGIATADV
                     GVAQLSMHSARELCGVEDAERFPRLLDAFFR"
BASE COUNT          502 a         1325 c         1418 g          543 t
ORIGIN      
        1 gcgctcggtg ggacggtccg ggtacgcctt ggcgtggcgg agggcgacct cggtggcgac
       61 ctcgtcgagg ccgatgccgc cgagcttgga ggtggtgccg gtgaagtact cgtcgatgag
      121 cccctggccg aggccgacgg cctcgaacac ctgggcgccg gtgtagctgg cgacggtgga
      181 gatgcccatc ttggacatga ccttgaggac gcccttgccg gcacccttga tgtagttctt
      241 gaccgccttg tccagaccga cgtcgggcag ggcgccgcgg gcggccatgt cctcgatggt
      301 ctcgaacgcg aggtacgggt tgatggccgc cgcgccgtag ccgatcagca gcgccatgtg
      361 gtgcacctcg cgggcgtcgc cggcctcgac cacgaggccg accgcggtgc gggtcttctc
      421 gcgcacgagg tggtggtgca cggccgccgt gagcagcagc gacgggatgg gggccagctc
      481 ccgggtggag tcgcggtccg agaggatcac gacgttggcg ccgccctcga tggcgtcgct
      541 cacctggcgg cgcaccgact cgatggcctc gcgcagggcg tcgccgcccc cggcgaccgg
      601 gtagagcccc tgcaccacca cggcgcggaa gtcgtcgacg ctgtcttcga cgtggatcag
      661 cttggcgagc tggtcgttgt cgaggatcgg gaacggcagc tggatctggc ggcacgagcg
      721 ggggccggga gcgagcaggt tctgctcggg gccgatggtc ccgccgaggc tggtgaccat
      781 ctcctcgcgg atggcgtcga gcggcgggtt ggtgacctgg gcgaagcgct gggcgaagta
      841 gtcgaacagc agccggggcc gctcggacag caccgcgatg ggcgtgtcgg tgcccatcga
      901 gccgagcgcc tcgccgccgg tgcgggccat gggggcgatg aggatcttct ggtcctcgat
      961 cgtgtagccg aaggtgagct ggcgccggac gacgtcgccg tgcgggtacc ggacgtgggg
     1021 ccgctcgggc agctcgtcga gctcgatgat cccctggtcg agccaggcct ggtacggcag
     1081 ctcggcggcg aggccggcct tgacctcgtc gtcttcgatg atgcggccct gggccgtgtc
     1141 gaccaggaac atcttcccgg gctggaggcg gcccttgcgc acgatctggt cggccgggat
     1201 gtcgagcacg ccggcctcgg agccgagcac gacaaggccg tcggcggtga cccagaagcg
     1261 gccggggcgc aggccgttgc ggtccagcac ggcgccgatc acggtgccgt cggtgaagct
     1321 cacgttggcc gggccgtccc acggctccat gagcgaggcg tggaagcggt agaacgcctt
     1381 gcgggccggg tccatgtggc ggtggttctc ccacgcctcg gggatcatca tcagcacggc
     1441 gtgcgggagc gggcggccgc ccaggtggag cagctcgagc acctcgtcga agctcgccgt
     1501 gtccgacgct cccggcgtgc agatcgggaa caggcgggag atgtcgccgg ggaagaggtc
     1561 gctggccagc agcgcctccc gggcgcgcat ccagttgcgg ttgccctcga ccgtgttgat
     1621 ctcgccgttg tgggcgaggt accggtacgg gtgggccagc ggccacgacg ggaacgtgtt
     1681 cgtggagaac cgggagtgca cgagggcgat ggcgctctcg acccgctcgt cggccaggtc
     1741 cgggtagaag tcccggagct gcggcgtggt cagcatgccc ttgtagacga tggtgcgagc
     1801 cgagaggctc gggaagtaga tccggcggtc gccggcgccg atctcgtgct cgatgcgctt
     1861 gcgcaccacg tacacccggc gctcgaggtc caggtcggtc acggtgccac cgggggcggc
     1921 gaggaagacc tggcggaacg cgggcatggc gtcgagggcg cccgagccga ggctgctggc
     1981 gtcgacgggc acgtcgcgcc aggcgaggac ctcgaggccc tcgctggccg cgatcttgcc
     2041 gacggcctcg acggccgagg tgacctccgc gtcgtcgccg gggaagaacc cgatgccggt
     2101 cgcgtaggcg cccgcggccg gcagctcgac gccgagctcg gcgcggtaga gcgcgtcggg
     2161 gacctggatc aagatcccgg cgccgtcacc ggtgttggtc tccgcacccg aggcaccacg
     2221 gtggtccagg tggacgaggg cggtgatgcc ctgctcgacc agctggtggg tgcgacggcc
     2281 gtgcatgtcg accacgaagg cgacaccaca gttctcgtgc tcgaaacggg ggtcgtagag
     2341 cccttgggca ggcgggggcg tgcgcatgcg gcgttgcttc cttgcgggag acgaaaggga
     2401 ctgctccccc cggtgagggg ctccccggga cgccgttggc ccgtgtcgca ggtcagacga
     2461 cactagccga ggtcggattc gaccctccaa tgggaaagca ggtcgtagcc tcacggtcga
     2521 tgagcgacgc gcaggacctg ttggccttca tcggggcggc tccctcgccg taccacgccg
     2581 tcggcgaggc ccgccgccgc ctggccgacg ccgggttcac cgcgctggac ctgaccgagc
     2641 cgtggccggc cgagccgggc cggtacctcg tcacgcgcgg cggagccctg gtggcctggg
     2701 aggtgcccga cgacgccggc cccgcacagc ggttccgcat cgtcggtgcc cacaccgaca
     2761 gcccgaacct gcgcatcaag ccgcggcccg acaccggctc ggccggctgg cagcagctcg
     2821 gcgtggaggt ctacggcggc gccctctgga acacctggct ggaccgggac ctcggtctgt
     2881 cgggccgggc ggtggtccgc gtcgacggtg cggtcgaaga gcggctcttc cactgcgact
     2941 gcccgctgct gcgcatcccg cagctcgcca tccacctcga ccgcgacgtc aacggccagg
     3001 ggctcaagct caacccgcag cagcacctga ccccggtctg gggcgtcggc cccgtggagc
     3061 ccggtgcctt cgccgcctac gtcgccgggg aggtggccgt ggcgccggcc gacgtcgtcg
     3121 cctgggacgc catggtccac gacctcaccc cgggcgcgac catcgggcgc cgccacgagc
     3181 tggtgtccac cgcccggctc gacaacctgc tctcgtgctg ggccggcgtc accgccatcg
     3241 ccgagccggg gagcggctcc gggcgccccg tgtcggtcct ctgcctcttc gaccacgagg
     3301 aggtcggctc cacgtcgtcc accggcgccg acggcgccct gctcgcccag gtgctcgagc
     3361 gcaccgtctc cgcccggggc ggcagccggg acgacctgct gcgggccctg gccgggtctg
     3421 tctgcgcctc ggccgacggc gcccacgcca cgcaccccaa ctacccggag cgccacgatc
     3481 ccggccacct ggtggccatc gacggcggac cggtcgtcaa ggtcaacgcc aaccagcgct
     3541 acgccaccga tgccgccggc cacgccgtgc tcatcgatgc cgccgatcgc gccggcgtcc
     3601 cccaccaggt cttcgtgagc cgcaacgacc agccctgcgg ctccaccatc ggcccgatga
     3661 ccgcggcccg cctcggcatc gccaccgccg acgtcggcgt ggcccagctg tcgatgcact
     3721 cggcccggga gctctgcggc gtcgaggacg ccgagcggtt cccgaggctg ctcgacgcct
     3781 tcttccgc
//
LOCUS       DATMFS010000645         5633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_182265, whole genome shotgun sequence.
ACCESSION   DATMFS010000645 DATMFS010000000
VERSION     DATMFS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182265"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1479
                     /locus_tag="VNQ33_12340"
     CDS             226..1479
                     /locus_tag="VNQ33_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWJ98943.1"
                     /translation="MRAPRLAVSAAAVLCLLAPLAACSGSSDSGSATKEDEPAEASTT
                     TTTAPAAEVYEGDAKGFYDVPDPLPDGPHGTLIRYEVMDGYEFDGATTYRIMYLSESL
                     AGDPIAVTGMATVPTAEAPEDGRPMVTVAHGTTGIADACSPSVTGKNAEMSLVENALS
                     EKYLVAATDYEGLGTPGRHPYLVGQSEGRSTIDAILAAGELPDAHPGTELGIVGYSQG
                     GHGALWANQVATEWAPGLEVVGTFSGAPASEIGVILAASEQVKGFGIMVIAGINAAYP
                     DADLDDILTPEGQAVLKSVDEGCVGDVFDAAGAAKGQLFVPDGNQTEPWSDLGKEQDA
                     GTEKTNDAPILLMHSDEDQTVPTFFIDQVEQRMCANGQVVERIDLPEGNHTSAAVPAY
                     EQALTWMQGLFAGKDVVKNTCPAPT"
     gene            complement(1573..2508)
                     /locus_tag="VNQ33_12345"
     CDS             complement(1573..2508)
                     /locus_tag="VNQ33_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWJ98944.1"
                     /translation="MARVVVTGGAGFLGSHLCHRLLDRGDEVVCLDNLITGSVANIED
                     LFGRPGFSFVELDVSTFIHVPGTVDAVLHFASPASPVDFERIPIQILKVGSLGTHNAL
                     GLAKAKGAKFFLASTSEVYGDPLVHPQPETYWGNVNPIGPRGVYDEAKRFAEAMTMAY
                     HRHHGLDVRIVRIFNTYGPQMRPDDGRAVSNFIVQALQGHDITVFGDGSQTRSFTYVD
                     DEVRGFLLLLDSDVVGPVNIGNPNEFTILQLAELVIEATGSSSKVVFEPLPVDDPTQR
                     KPDITKATELLDWKPEIELREGIAKATEYFRGVLR"
     gene            complement(2508..3794)
                     /locus_tag="VNQ33_12350"
     CDS             complement(2508..3794)
                     /locus_tag="VNQ33_12350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWJ98945.1"
                     /translation="MGSKIAIIGTGYVGLTTGACLSHLGHTVVCADVVPGKVESLSRG
                     EVPILEAGLDELVHDGIRDGLLSFVLGAENAVGDAEFVFLCLPTPQGADGAADLSFIK
                     EAAQQIGPYLQPETVVINKSTVPVGSAGEVAKKLERSDVHVVSNPEFLREGTAVDDFL
                     NPDRVVIGADDRGAADRVAALYDKLPGEVLITDPASAETIKYASNAFLATKISFVNAM
                     AAVCEEVGADVQDVVKGMGLDQRIGNRFLVPGPGWGGSCFPKDSHALVRIAENSGYQF
                     GLLRQVIAVNEEQFGRVANKISRFARGSLEGKTIAVWGLTFKAATDDLRDSPSLNIIN
                     RLLEVGATVRAYDPAVEDASDVRLAGIEICGDPYEAASGADVLAVLTEWPEFKDLSFE
                     KVGEVIGTKAVVDARNLLDRDAIKGLGFAYDGIGRS"
     gene            complement(3903..5381)
                     /locus_tag="VNQ33_12355"
     CDS             complement(3903..5381)
                     /locus_tag="VNQ33_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWJ98946.1"
                     /translation="MSRRKRARRPHRTWPQRLLITFNCCMVIVALVGAGSLAYAKRKV
                     SQIDRYELTNINDPTSPGFDSDQPRNFLLVGADSDDGLAADDPARAGRGDVGGIRSDT
                     IMVVRVDPKTNEAKVLSFPRDLWVDVPGFGKGRINSSLEFGGPDKLIETIKTNFDIDI
                     NHYVQVNFAGFKSLVDQIGGVPVYFATPVKDQGGLNVTSAGCNTLDGTGALAYVRARH
                     MKYLNENGRWKSIDPTSDLGRISRQQDFIKRVLRGAIKKGARNPRTLATLINTGVTNI
                     RLDQGTTAGDLIALGNAFRTFDPNALQTYNLPVTATVRSGADVLDLQTGGAEPILAQF
                     RGTGTEATGDLTPASIAVAVLNGSGVQNQAGDASSVLATAGFKMSEPGQAAGVMRTQV
                     RYAPGMEAQAALVARHLFADVDLIADQDVAEITVVTGPDFHAALVDPRPEKDFPVPTT
                     TTTTAVPGSESGSTDPDAGSTTTSSVVGYVPGAAPVGEPSCG"
BASE COUNT          868 a         1894 c         2015 g          856 t
ORIGIN      
        1 cgccaccccg acgatgtcga cgtcgtcgcg ctcggccagc gcccgcagtg cgcccagcgt
       61 ggacgtggcc gtgccccctg gcacccggtg ccagcactgc tcgaccgtga ccgccactcg
      121 gcgcctgcgt tccacacccg agattctggc ccctgccggc cgcatcggac accacgcctg
      181 cccccgacca tgaccggcgt gttgttgcag gtagcgtgca acttcatgcg cgcccctcgc
      241 cttgcggtct cggccgccgc cgtcctctgc ctcctcgctc ccctcgccgc ctgcagcggc
      301 tcctccgatt cgggctcggc gaccaaggag gacgagccgg ccgaggcgtc caccacgacg
      361 accacagccc ccgccgccga ggtctacgag ggcgacgcca agggcttcta cgacgtgccc
      421 gacccgctgc ccgatggtcc gcacggcacg ctcatccgct acgaggtcat ggacgggtac
      481 gagttcgacg gcgccacgac ctaccggatc atgtacctgt ccgagtcgct cgccggcgac
      541 ccgatcgcgg tcaccggcat ggccaccgtc cccaccgccg aggcgccgga ggacggccgc
      601 ccgatggtca ccgtcgccca cggcaccacc ggcatcgcag atgcgtgctc gccctcggtc
      661 accggcaaga acgccgagat gagcctggtc gagaacgccc tctccgagaa gtacctggtg
      721 gctgccaccg actacgaggg cctcggcacc cccggccgcc acccctacct ggtgggccag
      781 agcgaggggc gcagcaccat cgacgccatc ctcgccgccg gcgagctgcc cgacgcccac
      841 ccgggcaccg agctcggcat cgtcggctac tcgcagggcg gccacggcgc gctctgggcc
      901 aaccaggtgg ccaccgagtg ggcgcccggc ctcgaggtcg tcggcacgtt ctccggcgcc
      961 ccggccagcg agatcggcgt gatcctcgcc gcgtcggagc aggtgaaggg cttcgggatc
     1021 atggtcatcg ccggcatcaa cgccgcctac cccgacgccg acctcgacga catcctcacg
     1081 cctgagggcc aagcggtcct gaagtccgtg gacgagggct gcgtgggcga tgtgttcgac
     1141 gccgccggcg ccgccaaggg ccagctcttc gtgcccgacg gcaaccagac cgagccgtgg
     1201 tccgacctcg gcaaggagca ggacgccggc accgagaaga ccaacgatgc gccgatcctg
     1261 ctgatgcact ccgacgagga ccagaccgtc cccaccttct tcatcgacca ggtcgagcag
     1321 cgcatgtgcg ccaacggcca agtcgtcgag cgcatcgacc tccccgaagg caaccacacc
     1381 agcgccgccg tccccgccta cgagcaggcc ctgacctgga tgcaaggcct cttcgccggc
     1441 aaggacgtgg tgaagaacac ctgccctgcc ccgacctgat ctcgagccgc gagcgcgggt
     1501 acggctccgg agcgaagcga ggccccgggg agttcgagga gcggagcgag cgagcgacga
     1561 gagcgcggag cgtcagcgga gcacgccgcg gaagtactcc gtcgccttgg cgatgccttc
     1621 gcggagctcg atctcgggct tccagtcgag gagctcggtg gccttcgtga tgtcgggctt
     1681 gcgctgggtc ggatcgtcga ccggaagcgg ctcgaacacg accttcgagc tcgagccggt
     1741 ggcctcgatc accagctcag ccaactgcag gatggtgaac tcgttggggt tgccgatgtt
     1801 cacgggaccc acgacatccg agtcgagcag cagcaagaag ccgcggacct cgtcgtcgac
     1861 gtaggtgaag ctccgggtct ggcttccgtc gccgaacacg gtgatgtcgt ggccctggag
     1921 cgcctggacg atgaagttgc tcaccgcacg cccgtcgtcg ggccgcatct ggggcccgta
     1981 cgtgttgaag atgcggacga tgcggacgtc gaggccgtgg tggcggtggt aggccatggt
     2041 catggcctcg gcgaagcgct tggcctcgtc gtagacgccg cgggggccga tggggttcac
     2101 gttgccccag taggtctcgg gctgggggtg cacgagcggg tcgccgtaca cctcgctggt
     2161 ggaggcgagg aagaacttgg cgcccttcgc cttggccagc cccagggcgt tgtgcgtgcc
     2221 caggctgccg accttgagga tctggatcgg gatgcgctcg aagtcgacgg gcgatgcggg
     2281 actggcgaag tgcagcaccg catcgaccgt gccgggcacg tggatgaacg tcgagacgtc
     2341 gagctccacg aacgagaagc cgggccggcc gaacaggtcc tcgatgttgg cgacggaccc
     2401 ggtgatgagg ttgtcgaggc acacgacctc gtccccccgg tcgagcaggc ggtggcagag
     2461 gtgcgacccg aggaagcccg ctccgccggt gacgacgacc cgcgccatca gctccggccg
     2521 atgccgtcgt aggcgaagcc gaggcccttg atggcgtcac ggtcgagcag gttccgggcg
     2581 tcgacgacgg ccttggtgcc gatgacctcg ccgaccttct cgaagctgag gtccttgaac
     2641 tccggccact cggtgagcac ggcgagcacg tcggcccccg acgccgcctc ataggggtcg
     2701 ccgcagatct cgataccggc gagccggacg tccgaggcgt cctccaccgc cgggtcgtag
     2761 gcccgcacgg tggcgccgac ctcgaggagg cggttgatga tgttgagcga gggcgagtcc
     2821 cgcaggtcgt cggtggcggc cttgaaggtg agaccccaca cggcgatggt cttgccctcc
     2881 agcgagccgc gggcgaagcg ggagatcttg ttggccaccc ggccgaactg ctcctcgttc
     2941 acggcgatga cctggcgcag caggccgaac tggtagccgg agttctcggc gatgcgcacc
     3001 agcgcgtgcg agtccttcgg gaagcagctg ccgccccagc ccgggccggg gacgaggaag
     3061 cggttgccga tgcgctggtc gaggcccatg cccttgacga cgtcttgcac gtcggcgccg
     3121 acctcctcgc acaccgccgc catggcgttc acgaagctga tcttggtggc caagaaggcg
     3181 ttcgaggcgt acttgatggt ctcggccgag gccggatcgg tgatgagcac ctcgccgggg
     3241 agcttgtcgt agagggcggc gacgcggtct gccgcgcccc ggtcgtcggc gccgatgacg
     3301 acgcggtcgg ggttcaagaa gtcgtcgacc gccgtgcctt cgcggaggaa ctccgggttg
     3361 gagacgacgt gcacgtcgct gcgctcgagc ttcttggcca cctcgccggc cgacccgacg
     3421 gggacggtgg acttgttgat gaccaccgtc tcgggctgga ggtacgggcc gatctgctgg
     3481 gccgcctcct tgatgaacga gaggtcggca gcaccgtcgg cgccttgcgg ggtgggcagg
     3541 cacaagaaga cgaactcggc gtcgccgacg gcgttctccg ctccgaggac gaacgacaag
     3601 aggccgtcgc ggatgccgtc gtggaccagc tcgtcgagcc cggcctcgag gatcggcacc
     3661 tcgccccggg agagggactc gaccttgccg gggacgacgt cggcgcacac gacggtgtgc
     3721 cccaggtgcg acaggcaggc gccggtggtc agcccgacgt agcccgtgcc gatgatggcg
     3781 atcttgcttc ccacagatgc attcccgaga ttcgatggac gggcgcggcg agcgcacgtg
     3841 agccgctcaa ccgtagttcg cgccccttcg ggacacggca accggagcag tccgaccgga
     3901 ggtcagccgc aactcggctc gcccaccggt gccgcaccgg ggacgtaccc gacgacggag
     3961 ctggtggtcg tgctgccggc gtccggatcg gtggagccgg actccgagcc gggcaccgcc
     4021 gtggtggtcg tggtggtggg caccgggaag tccttctccg ggcgaggatc gaccagtgcg
     4081 gcgtggaagt cggggcccgt gaccacggtg atctccgcga catcctgatc ggcgatcagg
     4141 tcgacgtcgg cgaacaggtg gcgggccacc agggcggcct gggcctccat gcccggcgcg
     4201 tagcgcacct gggtgcgcat gacgccagcc gcctggcccg gctcgctcat cttgaagccc
     4261 gccgtggcca ggaccgacga ggcgtccccg gcctggttct ggacgccgct gccgttcaag
     4321 acggccacgg cgatggacgc gggcgtcagg tcgcccgtgg cctcggtacc cgtcccgcgg
     4381 aactgggcga ggatcggctc ggcgccaccg gtctggaggt cgagcacgtc cgcgccgctc
     4441 ctcaccgtcg ccgtgaccgg gaggttgtag gtctgcaggg cgttcggatc gaaggtccgg
     4501 aaggcgttgc ccagggcgat gaggtcgccg gcggtggtgc cctggtccag gcggatgttg
     4561 gtcacgccgg tgttgatgag ggtggcgagg gtgcgggggt tgcgggcgcc cttcttgatg
     4621 gcgccgcgga gcactcgctt gatgaagtcc tgctggcggc tgatacggcc caggtccgag
     4681 gtggggtcga tggacttcca gcggccgttc tcgttcaggt acttcatgtg ccgggcgcgg
     4741 acgtaggcga gggcgccggt gccgtcgagc gtgttgcagc ccgcgctcgt gacgttcagc
     4801 ccgccctggt ccttgaccgg cgtggcgaag tacaccggca cgccgccgat ctggtccacc
     4861 aggctcttga acccggcgaa gttgacctgc acgtagtggt tgatgtcgat gtcgaagttg
     4921 gtcttgatcg tctcgatcag cttgtcgggc ccgccgaact cgagcgacga gttgatccgg
     4981 cccttgccga agccggggac gtcgacccac aggtcacggg ggaacgagag caccttggcc
     5041 tcgttcgtct tggggtcgac ccggacgacc atgatcgtgt cggagcggat gccgcccaca
     5101 tcgcctcgtc cggcgcgggc cgggtcgtcg gcggcgaggc cgtcgtcgga gtcggccccg
     5161 acgagcagga agttccgggg ctggtcggag tcgaagcccg gcgaggtcgg gtcgttgatg
     5221 ttcgtgagct cgtagcggtc gatctggctg accttgcgct tggcgtaggc caaggagccg
     5281 gcacccacga gggcgacgat gaccatgcag cagttgaacg tgatgagcag gcgctggggc
     5341 cacgtccggt ggggacgtcg agctctcttg cgtcgcgaca tgggttcgta acctaatcgg
     5401 acaccctgat gacccaagag tcacccacgg tgaacattcg gtgcagaaac cttggacacg
     5461 cggcgcgagg cgggcttgac cttcgagcgg agcgagcccg ggggtttggg ggcagagctt
     5521 gcggagcccc caagtccagt tcggtgcgac gcagtcgtgc cgaactgcga aggcagccgg
     5581 gcgtcagccc gtcgatcgaa gctcgacgaa cggagcgaag cgagtgagga gaa
//
LOCUS       DATMFS010000646         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_161901, whole genome shotgun sequence.
ACCESSION   DATMFS010000646 DATMFS010000000
VERSION     DATMFS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161901"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VNQ33_12360"
     CDS             <1..717
                     /locus_tag="VNQ33_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ98947.1"
                     /translation="LRAMPAEHHPDHVSFLVIDTGIGIPEPEQARLFEPFSQLDETTT
                     RHYGGTGLGLGIVRGLVDLQGGTIELASEVGVGTVFRVTLPMAVGTQSTERGLAALVG
                     LRALVVDGNAVNRSVLAHTLHSWGFVVDQATSAEEALHQYAWTADSPDERYALAIIEH
                     KLDGMDGMELARHLRHQERTSSTVVLLLSAAADLSRQEAHDAGIESVLIRPVRTSYLL
                     RRIVDALITRPAEPVSTSHP"
     gene            729..1133
                     /locus_tag="VNQ33_12365"
     CDS             729..1133
                     /locus_tag="VNQ33_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ98948.1"
                     /translation="MHRILLAEDNKINQVVAVGVLKKLGYEVDIVEDGAAAVAACAET
                     QYGAVLMDVMMPNVDGYEATQLIRESERKRYLRPIPIIGLSARAMAGDREIALNAGMN
                     DYLTKPLRTPDLVDALGRWLDDEPVDGHLSPA"
     gene            1355..3052
                     /locus_tag="VNQ33_12370"
     CDS             1355..3052
                     /locus_tag="VNQ33_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWJ98949.1"
                     /translation="MSNLQLPWAGELRSPALAGVVPPGASSRSSGPLLGRGPRQRKPI
                     SAFKEGPTQKRFALLLAAKVLGVVALAGVIGLFSFLFETAAGAAPAQAATQAADLINP
                     LNTVWVLVAAFLVFFMQAGFMALEAGFARSKESANIMMECIFDTCLCGVLYWAVGFAF
                     EFGVGNALIGHSFFFLHHNSPDYNGTGVAFFAFVLFQFAFADTASTITSGSMIGRTSF
                     KGDILYSLAVSGLIYPITVHWIWGPGGWLGNTMGWFNGLVPDGIVFRDFAGSTAVHSV
                     GAVIALMGALALGPRLGRKFARDGGGPTAPHDVNIAAIGAVMLWFGWYGFNPGSTLSA
                     MDWEGIGRVAFNTTVAASAGGLVAVFWGYRKNKVWDIGLAINGFLGGLVAITAPCYWV
                     DPLGAVIIGLIAGVLIPLATDLVEHLRIDDPVGAVPVHGACGIWGTIAVGLFAAGHYG
                     VPTATGADNANPVTGLFYGGGTGQLQAQVIGNVACILMAGTLAFLVMFGLRKIPGSWN
                     LRLAPELELEGIDLTMHGLPAYHMDFGQGFTYTTPTGLPADLKTADLVGEPASGEEP"
     gene            3216..>3347
                     /locus_tag="VNQ33_12375"
     CDS             3216..>3347
                     /locus_tag="VNQ33_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWJ98950.1"
                     /translation="MATLVTAIVKPFMLEDVKAALRTAGVLGLTVSEVQGYGRQGGKN
                     "
BASE COUNT          487 a         1275 c         1056 g          529 t
ORIGIN      
        1 ttgcgggcca tgccggccga gcaccatccc gaccacgtgt cgttcctcgt gatcgacacc
       61 ggcatcggca tccccgagcc ggagcaggcc cgcttgttcg agccgttctc ccagctcgac
      121 gagacgacca cccgccacta cggcgggacc gggctcggcc tcgggatcgt ccggggcctg
      181 gtggacctgc agggcggcac catcgagctg gcgagcgagg tgggcgtcgg caccgtcttc
      241 cgggtcacgc tcccgatggc cgtcggcacc cagagcaccg agcgcgggct cgccgcgctg
      301 gtcggcctcc gcgccctggt ggtcgacggc aacgcggtca accgcagcgt cctcgcccac
      361 accctccaca gctggggctt cgtcgtggac caggccacct ccgcagaaga ggcgctgcac
      421 cagtacgcct ggacggccga ttcgcccgac gagcgctacg cactggccat catcgagcac
      481 aagctcgacg gcatggacgg catggagctg gcccgccacc tccgccacca ggagcgcacc
      541 tcctcgacgg tggtcctgct cctgagcgcc gccgccgacc tctctcggca ggaggcccac
      601 gacgccggga tcgagtccgt cctcatccga cccgtccgca cgtcctacct gctgcgtcgg
      661 atcgtcgacg ccctcatcac ccgaccggcg gagccggtct cgaccagcca tccctgaagg
      721 gaacagccat gcaccggata ctcctcgccg aggacaacaa gatcaaccag gtggtcgccg
      781 tcggcgtcct caagaagctg ggctacgagg tcgacatcgt ggaggacggg gcggctgccg
      841 tcgcggcatg cgccgagacc cagtacgggg cggtcctcat ggacgtgatg atgcccaacg
      901 tcgacggcta cgaggccacg cagctgatcc gcgagagcga gcggaagcgc tacctccgcc
      961 cgatcccgat catcggcctg agcgcccggg ccatggccgg tgaccgtgag atcgccctca
     1021 acgccgggat gaacgactac ctgaccaagc ccctgcgcac gccggacctg gtcgacgcgc
     1081 tcggccgctg gctcgacgac gagccggtcg acgggcacct cagccccgcc tgaagcccac
     1141 ctgaccccct cgctcggtgg ctggccgggc gacctcaggt gggccgctcg cgcctgaacc
     1201 gccgccgcgg ccctcgatgc tggcgccgcc ctcgatggtc agattccgtg caggtaggga
     1261 aacgcatccg ttcaggttcg tctcatgacc tggcgcgttg tcatttacgt gtcacgcatc
     1321 gagccgaaat gcttcatctc tacgttcagc ggccatgagc aaccttcaac ttccatgggc
     1381 tggcgagctg cggtcacccg cgctcgccgg cgtcgtacct ccgggagcgt cctcccgctc
     1441 gagcggaccg ctgctgggtc gtgggccacg gcagcgcaag cccatcagcg ccttcaagga
     1501 ggggccgacc cagaagcggt tcgccctcct gctggcggcg aaggtcctcg gggtcgtcgc
     1561 cctggctggc gtgatcgggc tcttctcctt cctgttcgag acggccgccg gcgcagcacc
     1621 ggcccaggcg gccacccaag ccgcggacct catcaacccg ctgaacacgg tgtgggtcct
     1681 cgtcgccgcc ttcctggtgt tcttcatgca ggccgggttc atggccctcg aagcgggctt
     1741 cgcccggtcg aaggaatccg ccaacatcat gatggagtgc atcttcgaca cctgcctgtg
     1801 cggcgtcctc tactgggccg tcgggttcgc gttcgagttc ggcgtcggca acgccctcat
     1861 cgggcactcg ttcttcttcc tgcatcacaa ctcgccggac tacaacggca ccggcgtggc
     1921 gttcttcgcc ttcgtgctgt tccagttcgc cttcgccgac accgcctcga ccatcacgtc
     1981 gggctccatg atcggccgca ccagcttcaa gggcgacatc ctctacagcc tggcggtgtc
     2041 gggcctcatc tacccgatca cggtccactg gatctggggt ccgggcggct ggctcggcaa
     2101 cacgatgggc tggttcaacg gcctggtccc cgacggcatc gtcttccgtg acttcgccgg
     2161 ctccaccgcc gtccactcgg tcggtgcggt catcgccctc atgggtgcgc tcgccctcgg
     2221 cccgcgcctc ggccgcaagt tcgcccggga cggcggcggc cccaccgctc cccacgacgt
     2281 gaacatcgcc gccatcggcg ccgtcatgct ctggttcggc tggtacggct tcaaccccgg
     2341 ctcgaccctc tctgccatgg actgggaggg gatcggccgg gtggcgttca acaccacggt
     2401 cgcggcgtcg gccggtggtc tcgtcgccgt cttctggggc taccgcaaga acaaggtgtg
     2461 ggacatcggc ctcgccatca acggcttcct cggcggcctc gtcgccatca ccgccccctg
     2521 ctactgggtc gacccgctgg gtgcggtcat catcggcctg atcgccggtg tcctcatccc
     2581 gctcgccacg gacctcgtcg agcacctgcg catcgacgat ccggtcggcg cggtcccggt
     2641 gcacggtgcc tgtggcatct ggggcacgat cgccgtcggg ctcttcgccg ccggccacta
     2701 cggggtcccg acggccaccg gcgccgacaa cgccaacccg gtcaccggcc tgttctacgg
     2761 cggcggcacc ggccagctcc aggcccaggt catcggcaac gtggcctgca tcttgatggc
     2821 cggcacgctc gccttcctcg tgatgttcgg cctccggaag atccccggaa gctggaacct
     2881 ccgcctggca cccgagctcg agctcgaagg catcgacctc acgatgcacg gcctgcccgc
     2941 ctaccacatg gacttcgggc agggcttcac gtacacgacc ccgacgggcc tgccggcgga
     3001 cctcaagacg gccgacctgg tgggcgagcc agcctccggc gaggaaccgt gacggcggcc
     3061 acggtggtcg tcgagcccga gtcgcaggca cggatcgagc ccgccggctt cgccaccgca
     3121 cccacccgag gacgcggccc cgtgccggcc gtggcctccc cgatgcccac cgccacctcg
     3181 cccgccactg ggcgccacca agacaggagc ccgtcatggc cacactcgtg accgccatcg
     3241 tcaagccgtt catgctcgaa gacgtcaagg ccgccctgcg gaccgccggc gtcctcggcc
     3301 tcaccgtcag cgaggtccag ggctacggcc gccagggcgg caagaac
//
LOCUS       DATMFS010000647         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_207395, whole genome shotgun sequence.
ACCESSION   DATMFS010000647 DATMFS010000000
VERSION     DATMFS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207395"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..841)
                     /locus_tag="VNQ33_12380"
     CDS             complement(182..841)
                     /locus_tag="VNQ33_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98951.1"
                     /translation="MNWLLLTFLGTAGAVIVACCIVALFVRHRLNRRHRVHPKVPTPA
                     PITWLADPRAAARLHRRLAKVGHTAGDVADDHRLPRKKLRRTVEQPEVVTLAEELRQQ
                     AVDLDAQIARTSTLPAPVRRQHLAQLAASVSEAELACVRLVSVSTQSRTHAVLANGTT
                     GQAPGGATGITGVAGQAHRLAEAHRILQQLDDRAGLAPAATPLGPLRAAPAPSTRRSA
                     R"
     gene            complement(838..2529)
                     /locus_tag="VNQ33_12385"
     CDS             complement(838..2529)
                     /locus_tag="VNQ33_12385"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HWJ98952.1"
                     /translation="MQQSFDDLGTPLNEVTFCVLDLETTGGTAADGGITEIGAVKLHG
                     GERVATFRTFVNPGHSIPLQITRLTGISPSMVADAPPPDAVLPTLLEFLGGAVIVGHN
                     VRYDLGYLNAAMERSGRPKLANRVLDTLRLARRLVRDEVPNCRLGTLADRFRLPNRPS
                     HRALDDALATGDLLHLLIERATAFGVCGLEDLMDLPTLAAHPQAGKLRLTDHLPKKPG
                     VYLFRDGGGRTLYVGKATDLRSRVRSYFSGDDRRKVGQLLREVERIDHVVCPSPLEAA
                     VLEVRLIHRLEPRFNRQSTTWRRSSYLKLTDEPFPRLSVVRAIREDGALYLGPLPSAK
                     AAKRVAEAIETAVPLRRCTGTPGRGGERTGPCAPAQLGVSTCPCAGAIAPEAYARLVA
                     TVRRGLLEEPALLLGPLEAKMHALAAEERFEEAADMRDRAAALVTALRRQQRFDTLRR
                     AGRVVMEVDGSSRAELLRGRLQRSWTVTKKGIAAVPLPLDLDPSAPDALEAPAPGDAS
                     APGAPLPKALADELACVAQWIDRSSHRIRVIHADGSLVPTGPPLPSFEPPVPFRR"
     gene            2742..2975
                     /locus_tag="VNQ33_12390"
     CDS             2742..2975
                     /locus_tag="VNQ33_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98953.1"
                     /translation="MSSAARYRCTSCGNLTRFDVTSTRQTRAYHHYTVGGELSIEDEQ
                     VLEETLEAVECRWCGASGDVEVLDESVDTASEG"
     gene            3102..>3966
                     /locus_tag="VNQ33_12395"
     CDS             3102..>3966
                     /locus_tag="VNQ33_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98954.1"
                     /translation="MLGFSRLSAAAYGVIARVIDEDGIFRARVAEAADEGEVGRASWL
                     WLHRPVGWLDDPAVSGGGPAPSTSERTAPRARRDDKAAANRLRKAAADADAARRRTAE
                     QLAGLRQAEAAVRAERDDLAASIEALQEERNAAVRRSKEVEAQLAAARRDLKVARQAT
                     MAAEEELARLRASEPGSAGAAPVVTPPAPVAPPAASVDPFDRGAVASSVAAAASAAAE
                     LAAALTDAASALADPTPPEVTAPAPEPTEARSAAKGEKKRGKKQRKASSRVGPALPPG
                     VFDGTAEAHRAL"
BASE COUNT          498 a         1387 c         1582 g          499 t
ORIGIN      
        1 gtcaccacgt cggccaggtc gtcgtggcgg gggacgcgca cgacggccac caggtcgacc
       61 tcgccggcca ccgagtacac ctcggcgatc ccctcgacct cggtcagctc gtcggccagc
      121 gccgccaccc gagccggctc cgacttgatc agcaagaacg cgtgcaccat ggccgcggac
      181 cctaccgggc agaccggcgg gtgctgggcg ctggggcggc gcgcagcggg cccagcgggg
      241 tggcggccgg ggcgaggccg gcgcggtcgt cgagctgctg gaggatccgg tgcgcttcgg
      301 ccagtcggtg ggcctggccg gcgacgccgg tgatgccggt cgctccgccc ggcgcttggc
      361 cggtggtgcc gttcgccagc accgcgtgcg tgcggctctg cgtgctgacc gagacgaggc
      421 ggacgcaggc gagctcggcc tcggacaccg aggcggcgag ctgggcgagg tgctggcggc
      481 gcacgggggc ggggagggtc gacgtgcggg cgatctgggc atcgaggtcg acggcctgct
      541 ggcgcagctc ctcggcgagg gtgaccacct cgggctgctc gaccgtccgg cgcagcttct
      601 tccgggggag ccggtggtcg tcggcgacgt cgccggcggt gtggcccacc ttggccaagc
      661 ggcggtgcag acgggcggct gcccgggggt cggccagcca cgtgatcggc gccggcgtgg
      721 ggaccttggg gtggacgcgg tgacggcggt tgaggcggtg ccgcacgaac agggccacga
      781 tgcagcacgc cacgatgacc gcgccggcgg tgccgaggaa cgtgagcagg agccagttca
      841 ccgcctgaag ggtaccggcg gctcgaacga gggcaagggc ggtccggtgg gcacgagcga
      901 cccgtcggcg tggatgacgc ggatgcggtg gctggagcgg tcgatccact gggccacgca
      961 cgccagctcg tcggcgaggg ccttgggcag cggagcgccc ggcgccgatg cgtcgccagg
     1021 ggccggagcc tcgagcgcat cgggagccga ggggtccagg tcgaggggga gcggcacggc
     1081 ggcgatgccc ttcttcgtga ccgtccagct gcgctggagg cggccccgca gcagctcggc
     1141 tcgggagctg ccgtcgacct ccatcaccac ccggccggct cggcggaggg tgtcgaaccg
     1201 ctgctggcgg cgcagggcgg tgacgagggc ggcggcccgg tcccgcatgt cggccgcctc
     1261 ctcgaaccgc tcctcggcgg cgagggcgtg catcttcgcc tccagggggc ccagcaggag
     1321 ggccggctcc tcgagcaggc cccgccggac ggtggcgacg agccgggcgt acgcctctgg
     1381 cgcgatggcg ccggcgcacg ggcacgtcga cacgccgagc tgggcggggg cgcagggccc
     1441 tgtccgctcg ccaccccggc ccggcgtgcc ggtgcaccgc cgcagcggca ccgccgtctc
     1501 gatggcctcg gccacccgct tggccgcctt cgccgacggc aggggaccga ggtacagcgc
     1561 gccgtcctcc cggatggctc gcaccaccga caggcgggga aacggctcgt cggtgagctt
     1621 caggtagctg gagcggcgcc aggtggtgga ctgccggttg aaccggggct ccaggcggtg
     1681 gatgagccgg acctcgagca cggcagcctc gagcgggctc gggcacacga cgtggtcgat
     1741 ccgctcgacc tcgcgcagca gctgccccac cttccgccgg tcgtcgccgg agaagtacga
     1801 gcggacgcgg gagcgcaggt cggtggcctt gccgacgtag agcgtgcggc cgccgccgtc
     1861 gcggaacagg tagacgccgg gcttcttcgg caggtggtcg gtgagccgca gcttgccggc
     1921 ctgggggtgg gcggcgaggg tcgggaggtc catgaggtcc tccaggccgc acacgccgaa
     1981 ggccgtcgcc cgttcgatca gcaggtggag gaggtcgccc gtggccagcg cgtcgtcgag
     2041 cgcacggtgc gacggccggt tggggagccg gaagcggtcg gccagggtcc cgaggcggca
     2101 gttcggtacc tcgtcgcgca ccaggcgccg ggcgaggcgc agcgtgtcga gcacgcggtt
     2161 ggccagcttc ggccgtccgg accgctccat ggcggcgttg aggtagccga ggtcgtagcg
     2221 gacgttgtgg ccgacgatga cggcaccgcc gaggaactcg aggagagtcg ggagcacggc
     2281 atcgggcgga ggagcgtcgg ccaccatcga cggggagatg ccggtgaggc gggtgatctg
     2341 cagcgggatg gagtgccccg ggttcacgaa cgtgcggaac gtcgccaccc gctcgccgcc
     2401 gtgcagcttc acggcgccga tctcggtgat cccgccatcg gcggcggtcc cgcccgtggt
     2461 ctccaggtcc agcacgcaga aggtgacctc gttcagcggc gtccccaggt cgtcgaagct
     2521 ctgctgcatg gctggatcat acgaacagat gttcccaaga acaagtgttc gtactgccgc
     2581 ttcgctccgg gctgctccgc ctcgccgggc cgtcgaggcc cggtcaccag cgggtcacga
     2641 tgttcgaacc gggatctgac gttcccgttg cgcctcgcgg cgcgcccgcg cgcgtccggg
     2701 ggctttccgg cgcgcccggt cccgccagta cgctggcctg cgtgtcttct gctgctcggt
     2761 accggtgcac gtcttgcggg aacctcacgc ggttcgatgt gacgtcgact cgtcagactc
     2821 gtgcgtacca ccactacacg gtgggcgggg agctctcgat cgaggacgag caggtgctgg
     2881 aggagaccct cgaggcggtc gagtgccgct ggtgcggggc gtcgggtgac gtcgaggtgc
     2941 tcgacgagtc cgtcgacacc gcgtccgagg gctgagccac ggccccgggt cctgccgtcg
     3001 acccgaaggc catccgcccg gcgtgtcgcc tggcggtcgc cgtcgcgcag aagggggagc
     3061 aggccgaccc tcggatcccc gcccccgtcg agctgcgccc ggtgctcggg ttctcccgcc
     3121 tgtcggctgc ggcctacggg gtgatcgccc gcgtcatcga cgaggacggc atcttccgcg
     3181 cccgcgtcgc cgaggccgcc gacgagggcg aggtcggtcg ggcgagctgg ctctggctgc
     3241 accggcccgt cgggtggctc gacgatccgg cggtgagcgg cggcggaccg gcaccgtcga
     3301 cgtcggagcg gaccgcgccc cgcgctcggc gtgacgacaa ggcggccgcc aaccggttgc
     3361 gcaaggccgc cgccgacgcc gatgccgccc gtcgccgcac cgctgagcag ctggccgggc
     3421 tgcgccaggc cgaggccgcc gtccgggccg aacgagatga cctggccgcg tcgatcgagg
     3481 cgctccagga ggagcggaac gcggccgtgc gccggtccaa ggaggtggag gcccagctcg
     3541 ccgccgcccg ccgggacctg aaggtcgccc ggcaggccac catggccgcg gaggaggagc
     3601 tcgcccgtct ccgggcctcg gaaccggggt ccgccggggc ggcgcccgtc gtgaccccgc
     3661 ccgcgccggt ggcgccgccg gcagcgtcgg tggacccgtt cgaccgcggc gcggtggcgt
     3721 cgtcggtggc cgcggcggcc tctgccgcgg ccgagctggc cgcagccctc accgacgcgg
     3781 cgtccgccct ggccgatccg acaccgccgg aggtcaccgc tcccgcgccg gagccgacgg
     3841 aggcccggtc ggcggcgaag ggcgagaaga agcggggaaa gaagcagcgc aaggccagca
     3901 gccgggtcgg tcccgccctg ccgcccggcg tgttcgacgg cacggccgaa gcgcaccggg
     3961 cgctgc
//
LOCUS       DATMFS010000648         3143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_230151, whole genome shotgun sequence.
ACCESSION   DATMFS010000648 DATMFS010000000
VERSION     DATMFS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230151"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VNQ33_12400"
     CDS             complement(<1..1419)
                     /locus_tag="VNQ33_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ98955.1"
                     /translation="MSPAPNSGSPTPPIEIAGGAASATGRLAAPVVLLDDAFLARLGE
                     ACADVRLDPGERADASRDWWPLAMVWATEGMVGQVAGAIARPSSVGEVAAVLRLCDDA
                     RIPITPAAGRSSVVGGTVPVHGGVLMDLTGLAGIESVDEQSGMVEVWAGTFGDLFEEE
                     LQEKYGLTVGHWPQSMNLSTVGGWLACKGAGQLSNRYGKIEDIVVGLEVVLADGTVFR
                     TGGQPRFAVGPDLTHLFLGAEGTLGVITRAWLQAWPKPTHTIRGAWSFPTFSAALDAM
                     RRIIQRGAPVAVLRLYDGIESNRNFGTELTDNVLLAYDEGDETMLTAAEAVLDEECAE
                     ARPLDDGLVEQWFGHRNNVDALEALISKGYVVDTMEISAPWSRLDAVYEAATAAISAV
                     PGTLAASAHQSHSYVTGACLYFTFAAQVAPEEREAYYVAAWDAGTRAVLEHGGSLSHH
                     HAVGLNRARFMPEVLGDGLGALA"
     gene            complement(1441..2445)
                     /locus_tag="VNQ33_12405"
     CDS             complement(1441..2445)
                     /locus_tag="VNQ33_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWJ98956.1"
                     /translation="MGRLRMPAEFDRHEGTAIAWPCRDEIYPGALMDEARDAHAQLAQ
                     AIARFEPVWVIADPADAEGAALRCGEGTTVVELPIDDSWFRDTGPVYVFDGTDRIALD
                     FTFNGWGEKFHPWDRDAAVASAWARAMGHPVHQVPLVFEGGSVTVDGMGTAATTTQCL
                     MHPNRNPGLTQTEIEDEVCDAFGLDAVIWLPYGLALDDDTDGHVDNVAAFSRPGTLLL
                     QGCDDPDEDDWVRLDVNRRVADGQPDAHGERIETVVVPALPFVERDGTRVPVPYLNLY
                     VGNGFVVVPTCGHPADADMVALIAEQFPGRETFALEIGSILAVGGGGIHCITQQIPAT
                     "
     gene            complement(2445..>3143)
                     /locus_tag="VNQ33_12410"
     CDS             complement(2445..>3143)
                     /locus_tag="VNQ33_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWJ98957.1"
                     /translation="EGHPTVEHFQSVAAELGIVLPVNVYERAGQAAFNTVVMIDADGT
                     ALGTYRKSHIPDGPGYTEKYYFNPGDTGFRAWDTAFGKVGIAICWDQWFPEAARCMAL
                     EGAEVLLYPTAIGSEPPDPTWDSSGHWQRVMQGHSAANLMPVVAANRYGFEQGATDRS
                     ITFYGSSFITDLTGATVAEAGRDGDAVLTATFDLDELRNQRNAWGLFRDRRPDLYGPI
                     ATLDGSTKPRLGGG"
BASE COUNT          441 a         1073 c         1191 g          438 t
ORIGIN      
        1 cgcgagggcg ccgaggccgt cgcccaggac ctcgggcatg aaccgggccc ggttgaggcc
       61 gacggcgtgg tggtggctga gcgagccgcc gtgctccagc acggcacggg tgccggcgtc
      121 ccaggcagcc acgtagtacg cctcgcgctc ctcgggcgcc acctgggcgg cgaaggtgaa
      181 gtacaggcag gcgccggtca cgtacgagtg cgactggtgc gccgaggcgg cgagggtgcc
      241 gggcacggcc gagatggcgg cggtggcggc ctcgtagacg gcgtcgagcc gagaccacgg
      301 cgccgagatc tccatggtgt cgaccacgta gcccttcgag atcagggcct ccagcgcatc
      361 gacgttgttg cggtggccga accactgctc caccaggccg tcatcgagcg ggcgggcctc
      421 ggcgcactcc tcgtcgagca cggcctcggc agcggtgagc atcgtctcgt cgccctcgtc
      481 gtaggccagg agcacgttgt cggtcagctc ggtcccgaag ttccggttcg actcgatgcc
      541 gtcgtacagg cgcaggacgg ccaccggcgc gccccgctgg atgatgcggc gcatggcatc
      601 gagggcggcc gagaacgtgg ggaagctcca ggcgccgcgg atggtgtggg tcggcttggg
      661 ccaggcctgg agccacgccc gggtgatgac gccgagcgtg ccctcggcgc cgaggaacag
      721 gtgggtgagg tccggaccca cggcgaagcg gggctgcccg ccggtgcgga acaccgtgcc
      781 gtcggcgagg acgacctcga gcccgacgac gatgtcctcg atcttcccgt agcggttcga
      841 cagctgtccg gcacccttgc aggccagcca gcccccgacg gtcgacaggt tcatcgactg
      901 cggccagtgc ccgacggtga ggccgtactt ctcctggagc tcctcctcga agaggtcccc
      961 gaacgtgccg gcccacacct cgaccatgcc ggactgctca tcgacggact cgatgccggc
     1021 gagcccggtc aggtccatca ggacgccgcc gtgaaccggg acggttccgc ccacgacgct
     1081 ggagcgcccg gcggccggcg tgatcgggat gcgagcgtcg tcgcagaggc gcaagacggc
     1141 ggcgacctcg ccgaccgacg acgggcgggc gatcgctccg gccacttggc cgaccatgcc
     1201 ctcggtggcc cacaccatgg cgagcggcca ccaatcgcgg ctggcatcgg cccgctcgcc
     1261 cggatcgagc cggacgtcgg cgcacgcctc gccgaggcgg gcgaggaagg cgtcgtcgag
     1321 gaggacgacc ggtgcggcga gccgcccggt ggccgaagcg gccccgccgg cgatctcgat
     1381 gggcggggtg ggcgatccag agttcggtgc agggctcatc gagaccgcac cctaggacgc
     1441 tcaggtggcg gggatctgct gggtgatgca gtggatcccg ccgccgccga cggcgaggat
     1501 cgatccgatc tccagggcga acgtctcgcg tccggggaac tgctcggcga tgagcgccac
     1561 catgtcggcg tcggccggat gcccgcaggt ggggacgacc acgaacccgt tgccgacgta
     1621 gaggttcagg taggggacgg ggacgcgggt gccgtcccgc tcgacgaacg gcagggccgg
     1681 gaccacgacg gtctcgatgc gctccccgtg ggcgtcgggc tgaccgtcgg cgacccggcg
     1741 gttgacgtcc agccggaccc agtcgtcctc gtcggggtcg tcgcagccct ggagcagcag
     1801 ggtcccgggc cgggagaagg cggcgacgtt gtcgacgtgg ccgtcggtgt cgtcgtcgag
     1861 ggccaggccg taggggagcc agatcacggc gtcgaggccg aacgcgtcgc agacctcgtc
     1921 ttcgatctcg gtctgggtga ggccggggtt gcggttcggg tgcatcaggc actgggtcgt
     1981 ggtggctgcc gtgcccatcc cgtcgacggt gacggacccg ccctcgaaca ccagcgggac
     2041 ctggtgcacc ggatggccca tggccctcgc ccacgccgag gcgacggcgg cgtcgcggtc
     2101 ccacggatgg aacttctcgc cccacccgtt gaacgtgaag tccaaggcga tgcggtcggt
     2161 cccgtcgaac acgtagaccg ggccggtgtc gcggaaccac gagtcgtcga tgggcagctc
     2221 gaccacggtg gtgccctcgc cgcagcgcag ggcagcacct tcggcgtcgg cggggtcggc
     2281 gatgacccac acgggctcga agcgggcgat ggcctgggcc agctgggcgt gggcgtcgcg
     2341 agcctcgtcc atcagcgccc ccgggtagat ctcgtcgcgg cacggccagg cgatggccgt
     2401 gccctcgtgg cggtcgaact cggcgggcat ccgcaggcgg cccatcaccc gcctccgagc
     2461 cgcggcttgg tcgagccgtc gagcgtggcg atcgggccgt acaggtcggg ccgccggtcg
     2521 cggaacaggc cccaggcgtt gcgctggttc cgcagctcgt cgaggtcgaa cgtcgccgtc
     2581 agcacggcgt cgccgtcgcg gccggcctcg gccaccgtgg cgccggtgag gtcggtgatg
     2641 aacgacgacc cgtagaaggt gatcgaccgg tcggtcgcgc cctgctcgaa cccgtagcgg
     2701 ttggcggcga cgaccggcat gaggttggcg gccgagtgcc cctgcatcac gcgctgccag
     2761 tggccgctcg agtcccacgt ggggtcgggc ggctcgctgc cgatggcggt ggggtagagc
     2821 aggacctcgg cgccctcgag cgccatgcag cgagccgcct ccgggaacca ctggtcccag
     2881 cagatggcga tgccgacctt gccgaacgcg gtgtcccacg cccggaaccc ggtgtcgccg
     2941 gggttgaagt agtacttctc ggtgtagccg gggccgtcgg ggatgtggct cttgcggtag
     3001 gtcccgagcg ccgtgccgtc ggcgtcgatc atcaccaccg tgttgaacgc ggcctggccg
     3061 gcccgctcgt acacgttgac gggcagcacg atgcccagct cagccgccac cgactggaag
     3121 tgctccaccg tggggtggcc ttc
//
LOCUS       DATMFS010000649        11233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_275793, whole genome shotgun sequence.
ACCESSION   DATMFS010000649 DATMFS010000000
VERSION     DATMFS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275793"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VNQ33_12415"
     CDS             complement(<1..1527)
                     /locus_tag="VNQ33_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWJ98958.1"
                     /translation="MDLTGLLPAAGRGRRTVLLAVVVGSAVGLAVAAFERLVVSGILS
                     RLFRAPLAVQATAPLVGLGLTALVLRVLASGASPSTADEYIKNFHERDRRLDLHPVFG
                     RVAASVLTLGSGCAMGLEGPSVYMGSAIGSAVQARTARWFSREDAKALLVAGAAAGVA
                     AIFKAPATGALFALEVPFQADLARRMLLPALFAASMSYLTFVAINGTTPLFPVAGSPA
                     LDFKDLASAVAIGLLAGLAARLFACAVRAAKRLSTTAHPIARILGAGIVLAAVILASK
                     ALTGDTLALGPGYRTITWAIEPRHAVGEVIGLLVLRGLATTTAVAGGGAGGLFIPLVI
                     EGALLGRAVGGAVGTPGSSLFPVLGVAAFLGAGYRVPLAAVMFVAESTGKPAFVVPGL
                     IAAAASQLTMGSRSVSTYQQARRAGHLEQRLDLPLASLVRTDAATVPPDATLSDLFTH
                     HMLGVRLLSVPVVDRATYLGTIDLHDVAAIDQTTWDETLVCDVMKTDRPVADLGWTIG
                     Q"
     gene            complement(1518..1976)
                     /locus_tag="VNQ33_12420"
     CDS             complement(1518..1976)
                     /locus_tag="VNQ33_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ98959.1"
                     /translation="MTDEDVVEAVLLASRALVAVAARSLGAAPEEVTLPQYRALVVLS
                     TQGTRSVSELATDLGTAASSVTRLCDRLVRKGLIVRAESSGNRRQTDLDVTAAGRDLV
                     QQVTAARLQEISRLVKAIPAARRPLVAEALSELGRAAGEVPEQVWSLGWT"
     gene            2256..3356
                     /locus_tag="VNQ33_12425"
     CDS             2256..3356
                     /locus_tag="VNQ33_12425"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWJ98960.1"
                     /translation="MRPLLPTPRGPISSALVAHLTDGEPLDVPSLRAVDPLVDDDFHL
                     ALWCCYELHHHGFDGVDDDLEWGPEVLRFRRTLERVFEGALRAEHHPDAVPSDPQLAL
                     RVIATWAGPPLAATVAEHGDLTHLQEVAIHRSAYQLKEADPHTWAIPRLSGVGRSALI
                     EIQTDEYGGGVPGEAHAELFAAAMEELGLSGELGHYVDRLPGTTLATDNLVALFGLNR
                     RLRGALVGHLALFELCSVTPMSRYLQAARRVGGLPALERFYEVHVEADVHHADLALHR
                     MVGGLVADEPELAADIVFGAAALSRVEARFAHAILRAWDDGQTSLRPSLEIALGVEAP
                     GGLAPDAVRAPVPRPSSTERADGTTGVVPSAG"
     gene            3423..3746
                     /locus_tag="VNQ33_12430"
     CDS             3423..3746
                     /locus_tag="VNQ33_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98961.1"
                     /translation="MPRSWSKKRERQYDHIKDGLEERGRSEDVAEEIAARTVNKERAR
                     AGEAKESSRSSTEDLSSGRRGGLRSHRGPGGRTRDQLYNEAKDKGIKGRSKMNKAQLA
                     KAVGR"
     gene            4227..5255
                     /locus_tag="VNQ33_12435"
     CDS             4227..5255
                     /locus_tag="VNQ33_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWJ98962.1"
                     /translation="MDWLRSIPAFPDVSTVVVVDGGGTGSRAAVATGDGELLGYAGGG
                     PTNSRSAGDADAAEHIRAVIAAALADAARPLAPTAVLVSSASVDTQAHADVLAAGVRK
                     VVPGPATISVVADTIGCWAATARCEPAVAVIAGTGSAVLAGALDQGCRRYGGWDYVLG
                     DEGSGYALGRAALREVLLVSEGRSDGSFLADAVRERLGIRETDELFDLVYKPEVDKAF
                     IASFATDVLRLATQGDPRSEDVVEEQVRLLADTVAAAFRDFTGLHSLGCFGGIWNVDT
                     YRTRFGRAVKAATGRQPAVVYPGDVAMAGSFRLVLRHGPGGAPGAAEDAAVDRFEAGL
                     LAVKGSPA"
     gene            5252..6040
                     /locus_tag="VNQ33_12440"
     CDS             5252..6040
                     /locus_tag="VNQ33_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWJ98963.1"
                     /translation="MSTYVAIGSVCWDEVEGDDERRLGGSVLFSTRVAQAAGWDGHIV
                     TSGTPELERALREAMPDTKVTVQPSATDTVMVFSRSADLGPQAVPTVAEPIDLTRSPV
                     DLGEADVVHLAPIMGEVTVALMAQVRGARFVGITPQGMLRRRDPRTNRLGLLAAPTTW
                     WANGTDAVVLSESEYGRIAEPLARPGRVVAVTRGERGCFGTSGNESVDLPGIPLEEIS
                     PTGTIGAGDVFAAALFVALADGLAFGAAMDAANRRAAAHVGGLA"
     gene            6204..7097
                     /locus_tag="VNQ33_12445"
     CDS             6204..7097
                     /locus_tag="VNQ33_12445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWJ98964.1"
                     /translation="MQFTLALAMCPPERVLPLARAAEEAGWDSVCFPDSVFYPETVSG
                     EYPFTADGKRFWPDDSPFVDPFVAMPAVGAATERIGLYTNVMKTPLREPLLVAKSIGS
                     IAALFPGRVSLGVGLSWIPEEFSWLGQEMKTRGKRLDEQIEIIRLVTAGGYAEYHGDH
                     YDFDRLRMEPPAPADHPIPIYVGGHSDAGIRRAARLGDGWIGAQASADEIDQHIARLG
                     AALDAEGRAGEPFEIKATPIVPATVDHMAGLAEAGLTDVITVPWFFSGGDPTDFQHQL
                     DSVAWFAETVIHPLRDLEIAP"
     gene            7094..7543
                     /locus_tag="VNQ33_12450"
     CDS             7094..7543
                     /locus_tag="VNQ33_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWJ98965.1"
                     /translation="MTTAFEYLDLPHPAADDRSARAASLRSRAFVQARDREGWLGIFA
                     EGAVVQDPVGVSFLDETGEGHHGREAIGRFWDDVISQNPVRMDIVSSHAGGDEVANYL
                     TITTTFPDGSSVAGEMIAIYRVGEDGRVESLRAFWEADQLRFTPAPS"
     gene            complement(7639..8088)
                     /locus_tag="VNQ33_12455"
     CDS             complement(7639..8088)
                     /locus_tag="VNQ33_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98966.1"
                     /translation="MKAFRRILLVVAALMAVVGVTVSLLPYDTSFRAPAIAGQAGLTA
                     EVSCPAPLVDLLTDDEPRDAWLNYAPDQGVVFESDRRAGGEYCGDTMRTRGILGLVVV
                     MGAVCFAAAAVVSGRLRPAPDDADPIDGPAPAHDRPDGPAGDARAAP"
     gene            complement(8085..10907)
                     /gene="leuS"
                     /locus_tag="VNQ33_12460"
     CDS             complement(8085..10907)
                     /gene="leuS"
                     /locus_tag="VNQ33_12460"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWJ98967.1"
                     /translation="MAEPTTTDADQLPEHRYDAALAQEIEAGWQTRWDAEGTFHTPNP
                     VGDLSDGFEAVADRPKQYVLDMFPYPSGAGLHVGHPLGYIGTDVFARYLRMTGHNVVH
                     TMGYDSFGLPAEQYALATNTHPRVTTESNIENMERQLRRLGLGYDRRRSVSTTDEAFY
                     RWTQWIFLQIFGSWYDDALGKARPIAELEAELDAGRVPDPGTNPTGKPWAGLDASERR
                     HVINGHRLAYLAEVPVNWCPGLGTVLANEEVTADGRSERGNFPVYRRPMKQWMLRITA
                     YADRLLDDLDLLDWTEAIKSQQRNWIGRSTGADVTFSTDAGPLTVFTTRPDTLFGATY
                     MVVAPEHPLVDALTTDDQRTAVQAYVAAAARRSDLDRQSEGKEKTGVFTGSLATNPVN
                     GESIPVYVADYVLMGYGTGAIMAVPAHDQRDFDFAQAFDLRIVPVIASPAEHDPLEQA
                     WTGDGAAINSANDEVSLDGRSVAEAKATIIEWLEGKGLGSGKVQYKLRDWLFSRQRYW
                     GEPFPIVFGADGEPQALPEAELPLVLPPMDDFSPKTSDDEDSEPEPPLGRITQWTTVE
                     LDLGDGVQTYRRETNTMPNWAGSCWYELRYLDPTNDEALVDPENERYWMGPQSPDDCG
                     GVDLYVGGVEHAVLHLLYARFWHKVLFDLGHVSSKEPFRRLFNQGMIQAHVYRDDRGF
                     PVPAADVVTEGDRYLYEGQEVTRELGKIGKSLKNAVAPDEMCEKYGADTLRLYEMFGG
                     PLDQSRPWDTTAVVGMYRLLQRIWRVIVDEDTGTPQISDDQPDDDTMRIRHRTVAAVR
                     DGMATLRFNTSIARITELTNHLTATYPDGGVPRSVAEDLVLLLAPLAPHIAEELWARL
                     GHASSLAYQDYPQFEEDWLVDASIELPVSVNGKVRSRIIVAADAGADALERAARDDEK
                     VAALLDGATIRKVIAVPGKMVNFVVG"
BASE COUNT         1628 a         3846 c         4139 g         1620 t
ORIGIN      
        1 ttgaccgatg gtccatccga gatcggccac gggccgatcg gtcttcatga cgtcgcacac
       61 gagcgtctcg tcccaggttg tctgatcgat ggctgccacg tcgtgcagat cgatggtgcc
      121 gaggtaggtg gctctgtcga cgacgggaac gctcaacagg cggaccccga gcatgtggtg
      181 agtgaacagg tcgctcaggg tggcgtcggg agggacggtc gcggcatcgg tgcgaacgag
      241 tgaggcgagc ggtaggtcga gtcgctgttc gaggtgcccg gctcggcgtg cctgctgata
      301 ggtcgagacc gagcgtgagc ccatggtgag ctgcgacgcc gcggccgcga tgaggcccgg
      361 tacgacgaag gcgggcttgc cggtggactc tgcgacgaac atgaccgcgg ccagcggtac
      421 ccggtagccg gcgccgagga aggcggctac tccgaggacg gggaacagcg aggacccagg
      481 tgtgccaacg gcgccgccga ctgcccgccc gagcagggct ccttcgatga cgagggggat
      541 gaacaggcca ccagcgccgc ctcctgcgac tgcggtggtg gtggcgagtc ctcgaaggac
      601 gaggagcccg atgacttcac cgacggcgtg acgaggctcg attgcccagg tgatcgtgcg
      661 gtagccgggt ccgagcgcga gggtgtcgcc ggtcagtgcc ttgctggcga ggatcaccgc
      721 ggcgagcacg atcccagcac caaggatccg ggcgatgggg tgggcggtgg tcgacaaccg
      781 cttcgcggcc cgcaccgcgc aggcgaagag ccgggcagcg agtccagcga gcaggccgat
      841 cgcgaccgcg gatgcaaggt ccttgaagtc gagcgcgggt gacccggcga ccgggaacag
      901 aggggtggtt ccgttgatgg cgacgaatgt gaggtagctc atcgatgcag cgaacagtgc
      961 cggcagcaac atgcgccggg ccaggtccgc ctggaacggc acctcgagcg cgaacaaggc
     1021 gcccgtggcc ggcgccttga agatggccgc gacacccgca gctgcaccgg cgacgagcag
     1081 cgccttggcg tcttcgcgtg agaaccaacg tgccgtccgt gcctgcactg ccgagccgat
     1141 ggctgagccc atgtacaccg atggaccctc caggcccatc gcacaaccag aaccaagggt
     1201 cagcacactc gccgcgacac ggccgaagac cggatggagg tcgagccggc ggtcgcgttc
     1261 gtggaagttc ttgatgtact cgtcggccgt cgacggcgat gctccagagg caaggacgcg
     1321 aagcacgagc gcggtgaggc cgagtccaac cagcggggcg gtggcttgca cggccagcgg
     1381 ggcacgaaag agacggctga ggatgccact caccacaagg cgttcgaagg ccgctaccgc
     1441 caagccgacg gccgatccga cgaccaccgc caacaacacg gtcctgcggc cgcggccggc
     1501 cgccggcagc aggccggtca ggtccatccc agcgaccaga cctgttcggg cacctcaccc
     1561 gcagcccgac ccagctcaga gagggcctcg gccaccagcg gacgccgagc cgctgggatc
     1621 gccttgacga ggcgggagat ctcctggagc cgggcggctg tcacctgctg aacgaggtct
     1681 cggcccgccg cggtgacgtc cagatcggtc tggcgccgat ttccggagct ttctgcccgg
     1741 acgatgaggc ccttgcgcac gaggcgatca cacagccggg tcacggacga ggccgccgtg
     1801 cccaaatcgg tcgccagttc ggacacggac cgcgtcccct gggtgctcaa caccaccagg
     1861 gcccggtact gcggcagcgt cacctcttct ggagcggcac cgagggaccg ggcggctacg
     1921 gccacaagtg cccgcgaagc caacagcact gcctcgacga cgtcttcgtc ggtcatcgaa
     1981 ccatcctaag ccaacctgtg caataggcaa taattgcatc ttgcacatat gcaacggcga
     2041 gtgtgccgca ggctcaacgc gcagcccgca tcgtccgtga gatcgcccag ggctcgccct
     2101 gaggccgatg taccggagat cgcttcgccg tgagttgagc gaggccgttc cggacggtgg
     2161 atccgaccgg ccgacgcggg tggatcggca cggcgcggat gagcgatcga ccctgacctg
     2221 gtgtgacgca cgtcgacatc gggaaggaac cgagcatgcg tcccctgctt cccacgccgc
     2281 gcggcccgat cagcagtgcc ctcgtggcgc acctgaccga cggcgagccg ctcgacgttc
     2341 cttcgctccg agctgtcgat ccgctggtcg acgacgactt ccacctcgcc ctgtggtgct
     2401 gctacgagct gcaccaccac ggcttcgacg gcgtcgacga cgacctggag tggggccccg
     2461 aggtgctccg gttccggcgc acgctcgagc gcgtgttcga gggtgcgctc cgtgccgagc
     2521 accaccccga cgccgtgccg tcggatccgc agctcgcgct ccgggtcatc gcgacctggg
     2581 ccgggccgcc gctggccgcc accgtcgccg agcacggcga cctgacccac ctccaggagg
     2641 tggcgatcca ccggtcggcg taccagttga aagaggctga cccgcacacg tgggcgatcc
     2701 cgcggctgtc gggcgtcggc cgttccgcac tcatcgagat ccagaccgac gagtacggcg
     2761 gcggtgtgcc gggcgaggcg cacgccgagc tgttcgccgc cgcgatggag gaactgggct
     2821 tgtcgggcga gctgggccac tacgtggacc ggctgcccgg caccacgctg gccaccgaca
     2881 acctcgtggc cctgttcggg ctcaaccgcc gcctgcgggg cgcgctggtc ggccacctcg
     2941 ccctgttcga gctgtgctcg gtcacgccga tgtcccgcta cctccaggcg gcacggcgcg
     3001 tcgggggcct gccagcgctc gagcgcttct acgaggtgca cgtcgaggcc gacgtccacc
     3061 acgccgacct cgcgctccac cgcatggtcg gcggcctcgt cgccgacgag cccgagcttg
     3121 cggccgacat cgtcttcggc gccgccgcgc tgtcgcgggt cgaggcgcgg ttcgcccacg
     3181 ccatcttgcg tgcgtgggac gacggacaga cctcgctgcg gccgtcgctc gagatcgccc
     3241 tcggcgtgga ggcgccgggc ggcctggctc ctgatgcggt ccgggccccg gtgccgcgac
     3301 cgtcgtcgac ggagcgggcc gacgggacga ccggcgtcgt gccgtcggcg ggctgaacgg
     3361 tcgtctcagg ccatggacga ccgggtatcc gaacagcacg caccccatca caggaggcac
     3421 cgatgccacg ttcctggagc aagaagcgag aacggcagta cgaccacatc aaagacgggc
     3481 tcgaagagcg ggggcggtcc gaagacgtgg ccgaggagat cgcggcgcgg accgtgaaca
     3541 aggagcgagc ccgcgccggc gaggcgaagg agtcgagccg cagctcgacc gaagacctgt
     3601 cgtcgggtcg gcgcggcggg ctgcgctccc atcggggccc aggcggccgc acccgtgatc
     3661 agctctacaa cgaggccaag gacaagggca tcaagggccg ctcgaagatg aacaaggccc
     3721 agctcgccaa ggcggtcggc cgctgagctg accgcggcgg atcaccggcg gcactgccgg
     3781 agcccgggga cgcggagacc ctcggagctc cggcggtgcc gtcgcggccc gagctgctcg
     3841 acacctcgaa gggcgaagga ggtgcccgcc aggttctgtg ccgtcggcac caaaccagaa
     3901 gcgcccgagg ccccggacca gcgggcggtc caggtcggct gatgaccggt ggccttcgct
     3961 ccgcgctggc gggccgctcg gacccgttgg gcgtggcatt cgcccgggcg gccgttggga
     4021 gccggacgag acccctgcgc cacggtggag gcgcaacagc cgctccgagc catgttgtcg
     4081 ccggtcatgc ggcgggcggt cctgccgacg atcctcgagc aggtccgcgg catcgagacg
     4141 acgggcgggt ggggccgcgg ctcgtgccga agggcccggg cgcgccgagc gaggcggcga
     4201 agggcgacca ctacgatcag ccgccgatgg actggctgcg atcgatcccc gcgttccccg
     4261 acgtctccac ggtcgtcgtc gtcgacggcg gggggacggg cagccgggcg gccgtcgcca
     4321 ccggcgatgg cgagctcctc ggctacgccg gtggcggccc caccaacagc cgctcggcgg
     4381 gcgacgccga cgccgccgaa cacatccgag cggtgatcgc ggcggctctg gccgacgcag
     4441 cgcggccgct cgcgccgacg gcggtgctcg tgagcagcgc ctcggtcgac acccaggccc
     4501 acgccgacgt cctcgccgcg ggtgtgcgca aggtggtgcc gggaccggcc accatctcgg
     4561 tggtcgccga cacgatcggg tgctgggcgg ccacagcccg gtgcgagccg gcggtggcgg
     4621 tgatcgccgg gacgggcagc gccgtgctcg ccggtgcgct ggaccagggg tgccggcgct
     4681 acggaggttg ggactacgtg ctgggcgacg agggttcggg ctacgccctc ggccgggctg
     4741 cgctgcgcga ggtgctcctc gtgagcgagg gccggtccga cggcagcttc ctggccgacg
     4801 ccgtgcggga gcggctcggc atccgcgaga ccgacgagct gttcgatctg gtctacaagc
     4861 cggaggtcga caaggccttc atcgccagct tcgccaccga tgtcctgcgg ctggcgacgc
     4921 agggtgatcc gcggtcggag gacgtggtgg aggagcaggt ccgtctgctg gccgacacgg
     4981 tggccgccgc gttcagagac ttcaccgggc tccacagcct cggctgcttc ggcggcatct
     5041 ggaacgtcga cacgtaccgc acccgcttcg gtcgggccgt caaggcagcc accggccggc
     5101 agcccgcggt cgtgtaccca ggcgatgtgg ccatggccgg ttcgttccgc ctcgtgctcc
     5161 gccacggccc tggtggcgca ccgggtgcag ccgaagatgc ggccgtcgac cgcttcgagg
     5221 ccggactgct ggccgtcaag ggctcccccg cgtgagcacc tacgtcgcca tcggctcggt
     5281 ctgctgggac gaggtcgaag gcgacgacga gcgcaggctc ggcggcagcg tcttgttctc
     5341 cacgcgagtc gctcaggccg cggggtggga cggccacatc gtcacctccg gcacgcccga
     5401 gctggaacgg gcgctgcgcg aggcgatgcc cgacaccaag gtcacggtgc agccttccgc
     5461 caccgacacg gtcatggtgt tcagccgctc cgccgacctc gggccgcagg ccgtccccac
     5521 cgtggcagag ccgatcgatc tcacccgctc gccggtcgac ctcggcgagg ccgacgtcgt
     5581 ccacctcgcg ccgatcatgg gcgaggtgac ggtggcgctc atggcccagg tgcgcggcgc
     5641 ccggttcgtg ggcatcaccc cgcagggcat gctgcgccgg cgcgacccgc ggacgaaccg
     5701 cctcgggctc ctcgccgcgc ccaccacgtg gtgggcgaac ggcaccgacg ccgtggtgct
     5761 gagcgagtcg gagtacggcc ggatcgccga gcccctggcc cggcccggcc gggtggtcgc
     5821 cgtcacgcgg ggcgagcggg gctgcttcgg caccagcggc aacgagagcg tcgacctccc
     5881 cggcatccca ctcgaggaga tctcgcccac cggcacgatc ggggcgggcg acgtgttcgc
     5941 cgccgccctg ttcgtcgccc tcgccgacgg cctcgccttc ggcgcggcga tggatgccgc
     6001 caaccgccgg gccgcggccc acgtgggcgg cctcgcctga cctttcaggt catgcgtgca
     6061 ccgtggcggt gcacgcatga cccgatgagg ccgtggtggc gtgggacccg ggatttcggg
     6121 tcgtctgggc acctccacgg tgcacaggtg acctgaaatc ggaggatccg gcggtggacc
     6181 tgacgatgcg tcagaatcgg ggggtgcagt tcaccctcgc cctcgccatg tgcccgcccg
     6241 agcgggtcct cccgctcgcc cgcgctgccg aggaggcggg ctgggactcg gtctgcttcc
     6301 cggactcggt cttctacccg gagacggtct cgggcgagta cccgttcacc gcggacggga
     6361 agcgcttctg gccggatgac tcgccgttcg tcgacccgtt cgtcgccatg ccggcggtcg
     6421 gtgccgccac cgagcgcatc gggctctaca cgaacgtgat gaagacaccc ctgcgcgagc
     6481 cgctgctggt cgccaagagc atcggctcga tcgccgccct cttccctggg cgggtgtcgc
     6541 tgggggtagg tctgagctgg atccccgagg agttctcctg gctcggccag gagatgaaga
     6601 cgcgcggcaa gcggctcgac gagcagatcg agatcatccg cctggtcacc gccggcgggt
     6661 acgccgagta ccacggcgac cactacgact tcgaccggct gcgcatggag ccgcccgctc
     6721 cggccgacca ccccatcccg atctacgtcg ggggccacag cgatgcgggc atccgccgag
     6781 ccgcccgcct gggcgacggc tggatcggcg cccaggcctc agcggacgag atcgaccagc
     6841 acatcgcccg gctcggggcc gcgctcgatg ccgagggccg ggcgggcgag ccgttcgaga
     6901 tcaaagcgac gcccatcgtg ccggccaccg tcgaccacat ggcgggcttg gccgaggccg
     6961 gactcaccga tgtcatcacc gtgccgtggt tcttctccgg tggcgacccc accgacttcc
     7021 agcaccagct cgacagcgtc gcctggttcg ccgagaccgt catccacccc cttcgagatc
     7081 tggagatcgc cccgtgacca ccgccttcga gtacctggac ctgccccacc cggccgccga
     7141 cgaccgttca gcccgtgctg cctcgctccg ctcgcgggcc ttcgtgcagg cccgcgaccg
     7201 cgaggggtgg ctcgggatct tcgccgaggg cgccgtggtg caggacccgg tgggcgtctc
     7261 gttcctcgac gagacgggcg agggccacca cgggcgggag gcgatcggga ggttctggga
     7321 cgacgtcatc tcgcagaacc cggtgcgcat ggacatcgtc tcgtcccacg cgggtggcga
     7381 cgaggtggcc aactacctga ccatcacgac gacgttcccc gacggctcga gcgtcgccgg
     7441 tgagatgatc gccatctacc gggtcggcga ggacggcagg gtcgagagcc tgcgggcctt
     7501 ctgggaggcg gaccagctcc ggttcacccc cgctcccagc tgatcccgca tccgcagggc
     7561 cgggccggcc ggccgggcgc ccgtcttcag tgccggagca gggtttctgc ggggtctggc
     7621 cctcggtgca tcgaccagct agggcgcggc gcgggcgtcg cctgccgggc catccggccg
     7681 gtcgtgggcc ggtgcggggc cgtcgatcgg gtcggcgtcg tccggtgcgg ggcggaggcg
     7741 tccgctcacg acggccgccg ccgcgaagca caccgcgccc atgacgacga cgagaccgag
     7801 gatcccgcgc gtccgcatcg tgtcgccgca gtactcgcca cccgctcggc ggtccgactc
     7861 gaacaccaca ccctggtccg gggcgtagtt cagccaggca tcgcggggct cgtcgtcggt
     7921 gaggaggtcg accagtggag ccgggcacga gacctcggcg gtgagcccgg cctggccggc
     7981 gatggcagga gcccggaagg acgtgtcgta gggcagcaac gagacggtga cgccgacgac
     8041 ggccatcagg gcggcgacga cgagcaggat ccgccggaac gccttcatcc gacgacgaag
     8101 ttgaccatct tgccggggac ggcgatcacc ttgcggatgg tggcgccgtc gagcagcgcc
     8161 gccaccttct cgtcgtcgcg cgctgcccgt tccagggcgt cggcaccggc gtcggcggcg
     8221 acgatgatcc gggaccgcac cttgccgttg accgacacgg gcagctcgat cgacgcgtcg
     8281 accagccagt cctcctcgaa ctgcgggtag tcctggtagg cgagtgacga ggcgtgcccc
     8341 aggcgggccc acagctcctc ggcgatgtgc ggggccagcg gcgccagcag caagacgagg
     8401 tcctcggcca ccgagcgggg tacgccgccg tcggggtacg tcgcggtgag gtggttggtc
     8461 agttcggtga tgcgggcgat cgaggtgttg aaccgcaggg tcgccatgcc gtcgcggacg
     8521 gcggcgacgg tgcggtggcg gatgcgcatg gtgtcgtcgt cgggctgatc gtcggagatc
     8581 tggggcgtgc cggtgtcctc gtcgacgatg acccgccaga tccgctgcaa caggcggtac
     8641 atgccgacga ccgcggtggt gtcccagggc cggctctggt cgagcgggcc gccgaacatc
     8701 tcgtagaggc gcagggtgtc ggcgccgtac ttctcgcaca tctcgtcggg ggcgacggcg
     8761 ttcttgagcg acttgccgat cttgcccagc tcccgggtga cctcttggcc ctcgtagagg
     8821 tagcggtcgc cctcggtcac cacatcggcc gccgggaccg ggaagccccg gtcgtcgcgg
     8881 tagacgtggg cctggatcat cccctggttg aagagccggc ggaacggctc cttcgacgag
     8941 acgtggccca ggtcgaagag caccttgtgc cagaagcggg cgtacagcag gtgcagcacg
     9001 gcgtgttcga cgccgcccac gtacaggtcg acgccgccgc agtcgtcggg ggactgcggg
     9061 cccatccagt agcgctcgtt ctcgggatcg accagggcct cgtcgttggt cgggtccagg
     9121 tagcgcagct cgtaccagca cgatccagcc cagttgggca tggtgttggt ctcgcgccgg
     9181 taggtctgca cgccgtcgcc caggtccagc tcgacggtgg tccactgggt gatgcggccc
     9241 agcggtggtt cgggctcgga gtcctcgtcg tcggacgtct tcggggagaa gtcgtccatg
     9301 ggcggcagga ccaggggcag ctcggcctcg ggcagcgcct ggggctcgcc gtcggcgccg
     9361 aacacgatgg ggaacggctc accccagtag cgctggcggc tgaacagcca gtcgcgcagc
     9421 ttgtactgca ccttgccgga acccaggccc ttgccctcga gccactcgat gatggtggcc
     9481 ttggcctcgg ccaccgagcg cccgtcgagg gagacctcgt cgttcgccga gttgatggcg
     9541 gcgccgtcgc cggtccacgc ctgctccagc ggatcgtgct cggcggggga ggcgatgacc
     9601 ggcacgatgc gcagatcgaa ggcctgggcg aagtcgaagt cgcgctggtc gtgggcgggc
     9661 acggccatga tcgccccggt gccgtagccc atcaagacgt agtcggcgac gtagacgggg
     9721 atggactcgc cgttgacggg gttggtggcc agcgagccgg tgaagacgcc ggtcttctcc
     9781 ttgccctcgc tctggcggtc gagatccgac cggcgggcgg ccgcggccac gtacgcctgc
     9841 accgcggtgc gctggtcgtc ggtggtcagg gcgtcgacga gcgggtgctc gggtgcgacg
     9901 accatgtagg tggcgccgaa gagcgtgtcg ggccgggtgg tgaagacggt cagcgggccg
     9961 gcatcggtgg agaacgtgac gtcggcaccg gtggagcggc cgatccagtt gcgctgctgg
    10021 ctcttgatgg cctcggtcca gtcgaggagg tccaagtcgt cgagcaggcg gtcggcgtag
    10081 gcggtgatgc gcagcatcca ctgcttcatc gggcggcggt agaccgggaa gttgccgcgc
    10141 tcggaccggc cgtcggcggt gacctcctcg ttggccagca cggtgcccag gccggggcac
    10201 cagttgaccg gcacctcggc caggtaggcg aggcggtggc cgttgatcac gtggcggcgc
    10261 tcggacgcgt cgagcccggc ccacggcttg cccgtcgggt tggtgccggg atcggggacc
    10321 cggccggcat cgagctcggc ctccagctcg gcgatggggc gggccttgcc cagcgcgtcg
    10381 tcgtaccagg agccgaagat ctgcaggaag atccactggg tccagcggta gaacgcctcg
    10441 tcggtggtgg agaccgagcg gcggcggtcg tagcccaagc ccaggcgtcg cagctggcgc
    10501 tccatgttct cgatgttgct ctcagtggtg acccgcgggt gggtgttggt ggccagcgcg
    10561 tactgctcgg cggggaggcc gaacgagtcg tagcccatcg tgtggaccac gttgtggccg
    10621 gtcatccgca ggtagcgggc gaacacgtcg gtgccgatgt agccgagggg gtggcccacg
    10681 tgcaggccgg cgccggaggg gtaggggaac atgtcgagca cgtactgctt gggtcggtcg
    10741 gccaccgcct cgaagccgtc ggacaggtcg cccacggggt tcggcgtgtg gaacgtcccc
    10801 tcggcatccc accgggtctg ccagccggcc tcgatctcct gggccagcgc cgcgtcgtag
    10861 cggtgttcgg gcagctggtc tgcgtcggtg gtggtcggtt cggccatggc ctcacggtac
    10921 ctgtcgccct ttgccgcccc gaagcccgaa cacggcgggt ggcgatgctc gccgcagtgg
    10981 aatggttcaa cgttcgcggc cgtgcggccg atagctggaa caggacctcc agccggtgca
    11041 ggccagccct gtgcctcctc caggcccgtc ggtggcggtg ccccgttggt cgaccgccgc
    11101 acgccgccct cgacagcgtg gtcgggggcg gcgccgcgct gtgctgacgc cgtggggcgg
    11161 gcgatgtcgc tggtgtctca ccagcgctgg cacggaccgg gtccgacgtg tgtgtcactg
    11221 gacctgcgta ggt
//
LOCUS       DATMFS010000650         3289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_68087, whole genome shotgun sequence.
ACCESSION   DATMFS010000650 DATMFS010000000
VERSION     DATMFS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68087"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="atpD"
                     /locus_tag="VNQ33_12465"
     CDS             <1..1112
                     /gene="atpD"
                     /locus_tag="VNQ33_12465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWJ98968.1"
                     /translation="PATFADAERWDIHRDAPDFDQLEPRKQVLETGIKVIDLLTPYLA
                     GGKIGLFGGAGVGKTVLIQEMIQRIAQNHGGVSVFAGVGERTREGTDLFLEMDESGVL
                     EKAALCFGQMDEPPGVRLRVALSALTMAEYFRDVQQQDVLLFVDNIFRFTQAGSEVST
                     LLGRMPSAVGYQPTLADEMGVLQERITSTRGRSITSLQAVYVPADDYTDPAPFTSFTH
                     FDGTTELSRDIAALGIYPAVDPLASSSTILAPEVVGDRHYNVAREVQRVLQRYKELQD
                     IIAILGLDELSEEDKITVARARKVQRFLSQPMYVAEVFTGLPGVTTPVEETVDSFEQL
                     LRGDLDDLPEQAFLNVGGADAARAKADELRKSAGE"
     gene            1120..1521
                     /locus_tag="VNQ33_12470"
     CDS             1120..1521
                     /locus_tag="VNQ33_12470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWJ98969.1"
                     /translation="MTMTVQVVSPERILWSGEAEMVTARTIEGGDISFQTGHAPFVGA
                     LQTGKVTIRPGDADPIVFAVHGGFVEVSNDEVSLLSDVSEAADQIDVERAQAALANAR
                     SALATDPLDAAAVDAVRRAEIRLDVAGAVAA"
     gene            1682..2548
                     /locus_tag="VNQ33_12475"
     CDS             1682..2548
                     /locus_tag="VNQ33_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98970.1"
                     /translation="MSQATEAQFALGSTTASSADELVKVRYARHAVTPIPWMIGAAVA
                     LLAAVWAIGWSKAGPGATDIVETSEWWSNIHSVLLPGSEGRLASSSEFRMWTALAIMI
                     VAALSIALWIGRIGRNLRPAHSPFGSVLPIVALPAWWVLPITLGATDAVNRSRGDALV
                     RYLIAFGILFAQFLLLRWPLLNRIWRAGRLPYDVASIVLWLPMLIPWSMLLLSTSFTL
                     LVTGKGDDPMDSAWRPTPSMADWAQNLTHATEAGVLILLVAVTVSQHLGILKDRSEER
                     VRRAAAADPLAP"
     gene            2571..>3289
                     /locus_tag="VNQ33_12480"
     CDS             2571..>3289
                     /locus_tag="VNQ33_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98971.1"
                     /translation="MNPYDPQPPADGEPRPASWPQAPGTTPVPPSSPAYPPGFSSYPP
                     GSSYPPGSPVPGPGYSPAAVGSYAPSLFVAPAGPSAGPAPASRLRSTARGTSPLWYLL
                     SGIGTFLLTFVVLFSALDSVSIEDDGVIDGGDAGLALLAIPLVIAALVLLFLWVRQIG
                     LNGGQFGGGLAFMAMLPWENLPFLGSSTTSSLGDMQVRSVAAVFVTVGAILLIRRPLI
                     VGVWRAGHLPAPVLAELLWVAT"
BASE COUNT          426 a         1244 c         1077 g          542 t
ORIGIN      
        1 atccggccac cttcgccgat gccgagcgtt gggacatcca ccgcgacgcc ccggacttcg
       61 atcagctcga gccccgcaag caggtgctgg agacgggcat caaggtcatc gacctgctca
      121 ccccctacct ggccggtggc aagatcggcc tgttcggcgg ggccggcgtg ggcaagacgg
      181 tgctcatcca ggagatgatc cagcgcatcg cccagaacca cggcggcgtg tcggtgttcg
      241 ccggcgtggg cgagcgcacc cgtgagggca ccgacctctt cttggagatg gacgagtccg
      301 gcgtgctcga gaaggccgcc ctgtgcttcg gccagatgga cgagcccccg ggcgtccgcc
      361 tccgcgtcgc cctctcggcc ctcaccatgg ccgagtactt ccgtgacgtg cagcagcagg
      421 acgtgctgct cttcgtggac aacatcttcc gcttcacgca ggccggctcc gaggtgtcca
      481 ccctcctcgg ccgcatgccc tcggccgtgg gctaccagcc caccctggcc gacgagatgg
      541 gcgtgctcca ggagcgcatc acctcgaccc gcggccggtc catcacctcg ctgcaggccg
      601 tgtacgtgcc cgccgacgac tacaccgatc cggcgccgtt cacctcgttc acccacttcg
      661 acggcaccac cgagctctcc cgcgacatcg ccgccctcgg catctacccg gccgtggatc
      721 cgctggcctc cagctcgacg atcctcgccc ccgaggtcgt gggcgatcga cactacaacg
      781 tcgcccgtga ggtccagcgg gtgctccagc gctacaagga gctccaggac atcatcgcca
      841 tcctcggcct cgacgagctg tccgaggagg acaagatcac cgtggcccgg gcccgcaagg
      901 tgcagcgctt cctctcgcag ccgatgtacg tggccgaggt gttcaccggc ctccccggtg
      961 tcacgacacc cgtcgaggag accgtcgact cgttcgagca gctcctccgc ggcgatctcg
     1021 acgacctgcc cgagcaggcc ttcttgaacg tgggcggcgc cgacgccgcc cgtgccaagg
     1081 ccgacgagct ccgcaagtcc gcgggggagt gatcacccca tgacgatgac ggtgcaggtc
     1141 gtctcccccg agcggatcct ctggtccggc gaggccgaga tggtcaccgc tcggaccatc
     1201 gagggcggtg acatctcctt ccagaccggg catgccccct tcgtgggcgc gctccagacc
     1261 ggcaaggtca ccatccgccc cggcgatgcc gacccgatcg tgttcgccgt ccacggcggg
     1321 ttcgtcgagg tcagcaacga cgaggtgtcg ctgctgagcg acgtctccga ggcagccgac
     1381 cagatcgacg tggaacgggc gcaggccgcg ctggccaacg cccgctcggc cctggccacc
     1441 gatccgctcg atgcggcggc agtggacgcg gtgcggcgcg ccgagatccg gctcgacgtg
     1501 gcgggtgcgg tcgcagcctg agggctgctc cagcagtacc atcccggggc gtccgggagg
     1561 agggcttccg gggcgggagg ggaccagacg aatgagtgac ggcgagcctc ctgctgaggc
     1621 gcccagtccg ggggaccgac cccagccgcg cccggacggt gcgtggtcct cgccggcagc
     1681 ggtgtcgcag gcgaccgagg cccagttcgc gctgggctcg acgacggcgt cctcggccga
     1741 cgagctggtg aaggtccgct acgcccgcca tgccgtcacg ccgatcccgt ggatgatcgg
     1801 cgccgcggtg gcgctgctgg ccgcggtctg ggccatcggc tggtcgaagg ccggtccggg
     1861 cgccaccgac atcgtcgaga cctccgagtg gtggtccaac atccattccg tgctgctgcc
     1921 cgggagtgag ggccggctgg cgtcctccag cgagttccgc atgtggaccg ccctcgcgat
     1981 catgatcgtc gccgcgctct cgatcgccct ctggatcggg cgcatcgggc gcaacctccg
     2041 gcccgcacac tcgccgttcg ggtcggtcct cccgatcgtc gccctcccgg cctggtgggt
     2101 gctgccgatc acgctcggcg ccaccgacgc ggtgaaccgg tcacgcggcg acgcgctggt
     2161 ccgctacctc atcgccttcg gcatcttgtt cgcccagttc ctgctcctgc gctggccgct
     2221 gctgaaccgg atctggcgag ccggtcgcct gccctacgac gtggcatcca tcgtcctctg
     2281 gctgccgatg ctcatcccgt ggtcgatgct cctcttgtcg acctccttca cgctcctggt
     2341 caccggcaag ggcgacgatc cgatggactc ggcctggcgc cccaccccgt cgatggctga
     2401 ctgggcgcag aacctcacgc acgccaccga ggccggcgtg ctgatcctcc tggtcgcggt
     2461 caccgtgagc cagcacctgg ggatcttgaa ggaccgttcg gaagagcgcg tccgccgcgc
     2521 cgccgccgct gacccgctgg cgccttgatg ccaccgtccc cggagatccg atgaacccgt
     2581 acgatcccca gcctcctgcc gacggcgaac ctcgtccggc ctcgtggccc caggctccgg
     2641 gcacgacccc ggtacccccg tcgagccccg cctacccgcc ggggttctcg agctacccgc
     2701 cgggttcgag ctacccgccg ggttcgccgg ttccgggtcc cgggtactcg cccgcagccg
     2761 tcgggtcgta cgctccttcg ctcttcgtcg cgccagccgg accctcggcc gggccggctc
     2821 ccgcgtcccg cctccgctcg acggcgcggg ggacatcgcc cctctggtac ctgctgagcg
     2881 gcatcgggac gttcctgctc accttcgtcg tgctgttctc cgcgctggac tcggtctcga
     2941 tcgaagacga cggcgtcata gacggcgggg atgccggcct cgccctgctc gccatcccgc
     3001 tggtcatcgc cgcgttggtg ctgctgttcc tctgggtccg gcagatcggc ctcaacggcg
     3061 ggcagttcgg tggtggcctc gccttcatgg ccatgctccc ctgggaaaac ctcccgtttc
     3121 tcgggtcgtc cacgacctcg agcctcggcg acatgcaggt ccggtccgtg gcggcggtct
     3181 tcgtcaccgt cggcgcgatc ttgttgatcc ggcggccgct catcgtcggg gtctggaggg
     3241 ccgggcacct cccggctccg gtcctcgccg agctcctctg ggtcgccac
//
LOCUS       DATMFS010000651         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_138629, whole genome shotgun sequence.
ACCESSION   DATMFS010000651 DATMFS010000000
VERSION     DATMFS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138629"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VNQ33_12485"
     CDS             complement(<1..563)
                     /locus_tag="VNQ33_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98972.1"
                     /translation="MPIRVVHLVAKTHLDLGFTALAAEVEQQYLEEFFPRAVAVADEL
                     RQRGGSEHLVWTTGSWIVHRALRQADAARAAAVRSAIRTGALAWHALPLTTHTELMDA
                     PSFRAGLGISQELDQRFGRTTTAAKMTDVPGHTRAMVPLLAEAGVTFLHLGVNPAWPV
                     PDVPPTFRWRSPASSPWPTPPAPPAPPA"
     gene            652..3282
                     /locus_tag="VNQ33_12490"
     CDS             652..3282
                     /locus_tag="VNQ33_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWJ98973.1"
                     /translation="MRTFWSNLAVQLGKRAGLVSVIGLLVTGILGFGITKLQFATGQD
                     SYLNKSDQVAKDNVAYQKLFGGQIMIVLFTMDEGAAGGGKVPVTGLATPENQFVIDQA
                     KAEIEKNPHVEAVLTPLDTLDYSANLLSRTPDDPTNAKAFDPLESIANTALTDAQDAE
                     KPGSAGATARSADFGKTAGRLAPFLASTPDQRTLDNPDWVNILLLDNEGTIRKPLRSS
                     FFDEDHAQMIVRLKGNADIEDEGAGAIAVKDAWTPHTVKEGAELPDGTTCKTDGGCQL
                     GEITGGTTLVTGAPLLLKDINDYLRGGILSLGAIAVAIMMVILLVFFQVRWRLLPLAV
                     VLIGVIWAFGTAGWVGIPLSIVTIAGLPVMLGVGIDYAIQMHARIEEEVIIDRAEHPI
                     QETARQLGPALLVVTFDAVFAFAALRFAKVPMIREFALLLCIGVALICFCSIIWPLAI
                     LGIREYKSPTTGRDFREGYLGRLTVFLGDLPTWSAIPIMLLSLLIFVGGIAVEGKLTL
                     QTDPIQWVNQKSQNRLDVATLEEETGASSELGIFVTGDSKEDLFTDDTMKWLDDFTDD
                     ALDRYQDKLLVASSVATPLSDLVYLDGASNNTPSGALTKASYEAAPDSVKAFTVASGD
                     DPALNILFITKPGDLNERAEMVNDMRDRFAESGKDGAKPPAGISATPSGLAVVGVGLL
                     ENLESNRVQLTYLSIFFVFAFLTIRLRSVVRSLLSLVPVLIAVGLASLVAFAFSLKLS
                     PMTAVGGPLVIAVCTEFTSLIILRFLEERERGFSPRAAAEITAARTGRAFIVSGMTAV
                     VGVAVIATSSLPLLRDFGIIVAMNVVVALLSALILLPPVIIWAEERGWVSRGMIDPEK
                     LGNKSRDERTPTPDLTDAFR"
BASE COUNT          512 a         1187 c         1153 g          529 t
ORIGIN      
        1 gcgggtggcg cgggtggggc gggtggcgtg ggccaggggg acgaggcagg tgagcgccag
       61 cggaaggtgg gcgggacgtc cgggacgggc caggcggggt tcacgccgag gtggaggaac
      121 gtgacgccgg cctcggcgag cagcgggacc atggcccggg tgtggccggg gacgtcggtc
      181 atcttggctg ccgtggtggt gcggccgaag cgctggtcga gctcctggct gatgccgagg
      241 ccggcgcgaa acgacggtgc gtccatcagc tccgtgtgcg tcgtgagcgg cagcgcgtgc
      301 caggcgaggg cgccggtccg gatggcggac cgcacggccg cggcccgtgc cgcgtcggct
      361 tggcggaggg cgcggtgcac gatccacgag cccgtcgtcc acaccaggtg ctcggacccg
      421 ccccgctggc ggagctcgtc ggccacggcg accgcccggg ggaagaactc ctcgaggtac
      481 tgctgctcga cctcggcggc gagggccgtg aagcccaggt ccagatgggt cttggccacc
      541 aggtggacca cgcggatggg cacggcgcca cggtaccgac ccgacacctt gcgttcacct
      601 gcgaggtaag gtcgcccgcc ctcgttctgg cccctcctcc tggagctccc catgcgcacg
      661 ttctggtcga acctcgcggt tcagctcggc aagcgagccg gcttggtgtc ggtcatcggt
      721 ctgctggtca ccggcatctt gggcttcggc atcacgaagc tgcagttcgc caccggccag
      781 gacagctacc tgaacaagag cgaccaggtg gccaaggaca acgtggcgta ccagaagctc
      841 ttcggcggcc agatcatgat cgtcttgttc acgatggacg aaggcgcggc gggcggcggc
      901 aaggtccccg tcacggggct ggccacgccg gagaaccagt tcgtgatcga ccaggcgaag
      961 gccgagatcg agaagaaccc gcacgtcgag gccgtcctca ccccgctcga cacgctcgac
     1021 tacagcgcca acctgctgtc gcgcaccccc gacgatccca ccaatgccaa ggcgttcgat
     1081 ccgctcgagt cgatcgccaa caccgccctc accgacgccc aagacgccga gaagcccggc
     1141 tccgcgggcg ccacggcgcg ctcggccgac ttcggcaaga ccgccgggcg cctggcgccg
     1201 ttcctggcct ccaccccgga ccagcgcacg ctggacaacc ccgactgggt caacatcctc
     1261 ctgctcgaca acgagggcac catccgcaag ccgctgcggt cgtcgttctt cgacgaggac
     1321 cacgcccaga tgatcgtacg gctcaagggc aacgccgaca tcgaggacga aggcgctggc
     1381 gccatcgccg tcaaggacgc ctggaccccg cacacggtca aggagggcgc cgagctgccc
     1441 gacggcacca cctgcaagac cgacggcggc tgccagctgg gtgagatcac cggcggcacc
     1501 accctcgtga ccggcgcgcc cctcctgctg aaggacatca acgactacct gcgcggcggc
     1561 atcctctcgc tcggcgccat cgccgtggcg atcatgatgg tcatcctcct ggtgttcttc
     1621 caggtgcgct ggcggctgct gccgctggcg gtggtcctca tcggcgtcat ctgggcgttc
     1681 ggcaccgcgg gctgggtggg catacccctg tccatcgtca cgatcgccgg actccctgtg
     1741 atgctcggcg tgggcatcga ctacgcgatc cagatgcacg cgcgcatcga ggaggaggtg
     1801 atcatcgacc gggcggagca ccccatccag gagacggcga gacaactcgg gccggccctg
     1861 ctcgtggtga cgttcgacgc cgtcttcgcc ttcgccgccc tgcgcttcgc caaggtgccg
     1921 atgatccggg agttcgccct cctgctgtgc atcggcgtcg ccttgatctg cttctgctcg
     1981 atcatctggc cgctcgccat cctcggcatc cgcgagtaca agtcgcccac cacgggccgg
     2041 gacttccgcg agggctacct cgggcgcttg accgtgttcc tgggcgacct gcccacctgg
     2101 tcggccatcc cgatcatgct gctcagcctg ctgatcttcg tgggcggcat cgccgtcgag
     2161 gggaagctca ccttgcagac cgacccgatc cagtgggtca accagaagag ccagaaccgc
     2221 ctcgacgtcg ccacgctcga ggaggagacc ggggcgtcgt ccgagctggg catcttcgtc
     2281 acgggcgact cgaaggagga cctcttcacc gacgacacca tgaagtggct cgacgacttc
     2341 accgacgatg cgctcgaccg ctaccaggac aagctcctcg tggcctcgtc ggtggccacc
     2401 ccgctgtcgg acctcgtgta cctcgacggg gcgtcgaaca acacgccgag cggtgcgctg
     2461 accaaggcgt cctacgaggc tgcgcccgat tcggtgaagg ccttcaccgt ggcgagcggc
     2521 gacgatccgg cgctcaacat cctcttcatc accaagcccg gcgacctcaa cgagcgggcc
     2581 gagatggtga acgacatgcg ggaccgcttc gccgagtccg gcaaggacgg cgccaagccg
     2641 ccggccggca tctcggccac gccgtcgggc ctcgccgtgg tgggcgtcgg cctcctggag
     2701 aacctggagt ccaaccgggt ccagctcacc tacctgtcga tcttcttcgt gttcgccttc
     2761 ctcacgatcc ggctccggag cgtcgtgcgg tcgctgctgt cgctggtgcc ggtgctcatc
     2821 gccgtgggcc tggcgtcgct ggtcgcgttc gccttcagcc tcaagctgag cccgatgacc
     2881 gcggtgggcg gcccgctcgt gatcgccgtc tgcacggagt tcacgtcgct gatcatcctg
     2941 cggttcctcg aagagcgaga acgagggttc agtccccgcg ccgcggccga gatcaccgcc
     3001 gcccgcaccg gccgggcgtt catcgtgtcg ggcatgaccg ccgtcgtcgg cgtcgccgtc
     3061 atcgccacct cgtcgctgcc gctgctgcgg gacttcggca tcatcgtcgc catgaacgtg
     3121 gtggtggcgc tgctgtccgc cctgatcctg ctgcccccgg tgatcatctg ggccgaggag
     3181 cggggctggg tctcgagggg gatgatcgac cccgagaagc tcggcaacaa gagccgcgac
     3241 gagcgcacgc cgacgccgga cctcaccgac gcgttccgct aggcgtcgtc gtccacgatg
     3301 gtgatggtgc tgctgagccg ggtgccgtag atgaagccgg ggcctgcggt ggcccggccc
     3361 gagaacgtga accgggtgct g
//
LOCUS       DATMFS010000652         3149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_92002, whole genome shotgun sequence.
ACCESSION   DATMFS010000652 DATMFS010000000
VERSION     DATMFS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92002"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VNQ33_12495"
     CDS             complement(<1..822)
                     /locus_tag="VNQ33_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWJ98974.1"
                     /translation="MVEIWSAEHKIILERRLWLAVLRAQAALGVDVADGVIDDYEAVV
                     DQVDLESIAARERVTRHDVKARIEEFSDLAGHEHIHKGMTSRDLTENVEQLQVRDALV
                     LVRSRMLTVLARLAERAAEHGTTVITGRSHNVPAQATTLGKRFANAGQELLVALQRVD
                     ELIARYPLRGIKGPVGTQQDMLDLLGSPDAVARMEEAVAQHLGFRAALHNVGQVYPRS
                     LDLDVVSALVQAAAGPSSLATTLRLMAGQELVTEGFKEGQVGSSAMPHKMNSRSCE"
     gene            981..1370
                     /locus_tag="VNQ33_12500"
     CDS             981..1370
                     /locus_tag="VNQ33_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98975.1"
                     /translation="MRHFEEPVVAHCRTCARPYCSRCLVYSFGPDKPPFCVGCALNAS
                     GVRNKNMPVPVAAQAAPGPDRKTERAQRKAEKAEAKASARALKRAAKRGEVTAEAAVP
                     RSSNVPVPKGLPTPSARFAPQTEHAVN"
     gene            complement(1363..1824)
                     /gene="purE"
                     /locus_tag="VNQ33_12505"
     CDS             complement(1363..1824)
                     /gene="purE"
                     /locus_tag="VNQ33_12505"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWJ98976.1"
                     /translation="MSQYKVAIIMGSPNDRSKMEPAAETLAGFGIEAEVAVMSAHRTP
                     AKVAAFVGSARAEGYAAIIAGAGMAAHLAGAAAAHTTLPVIGVPLSGGALNGVDALYA
                     TVQMPKGIPVATVAVDGSVNAALLVVQMLAISDADLEAKLVADREARASVS"
     gene            complement(1970..>3149)
                     /locus_tag="VNQ33_12510"
     CDS             complement(1970..>3149)
                     /locus_tag="VNQ33_12510"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWJ98977.1"
                     /translation="GVSLSGPATNCGRSLGTLARGASVVVRCTTNAGAFLDRTYRFRA
                     TSGGKTAVSNRVEVRTLKHHTNYWSTTLPNAPKAGTVALAAGNKIDAPVALAPPTASA
                     GPNTPPVRWLVTSVFEKGRGVPTQGSVARPTGKVTFDLAEGVPVRVALAARNGAGTGA
                     RTPLTPFLTPRPTVNWPYASPTEQVEGILQVVDGRAPTADELTWYLARLQAGASPADV
                     IADRLDRGRWPTQVEPLIRLYAAYFGRPPDSSGLRFWSEQRANGRTLDRISSTFAGSS
                     EFIRKSGSLDNRAFVRFVYSSVLGRSPDAPGLAYWVARLDAGWTRGRVMTTFSESSEG
                     KRKLAPQVGPTLVTAALLGRPPSVAEAQPATEWLAGGGSLNTVIDGVRSSQAYADHVG
                     "
BASE COUNT          447 a         1108 c         1167 g          427 t
ORIGIN      
        1 ctcgcacgac cgggagttca tcttgtgggg catcgccgag gagccgacct gtccctcctt
       61 gaacccctcg gtgaccagct cctggccggc catgaggcgc agcgtggtgg ccaggctgga
      121 ggggccggcc gccgcctgga ccagcgccga caccacgtcg aggtcgagcg accgggggta
      181 cacctggccg acgttgtgca gcgcggctcg gaagccgagg tgctgggcga cggcctcctc
      241 catgcgggcg accgcatcgg gtgatccgag caggtcgagc atgtcctgct gcgtgcccac
      301 cggccccttg atcccacgga gcgggtaccg ggcgatgagc tcgtccaccc gctggagcgc
      361 cacgagcagc tcctgcccgg cgttggcgaa ccgcttgccc agcgtggtgg cctgcgccgg
      421 cacgttgtgg ctgcgcccgg tgatcaccgt ggtcccgtgc tcggcggccc gctccgcgag
      481 ccgggccagc accgtgagca tccggctgcg gaccaggacc agggcgtcgc ggacctggag
      541 ctgctcgacg ttctcggtca ggtcgcgcga ggtcatcccc ttgtggatgt gctcgtggcc
      601 ggccaggtcc gagaactcct cgatgcgagc cttcacgtcg tgacgggtga cccgctcgcg
      661 cgccgcgatc gactcgaggt cgacctggtc gaccacggcc tcgtagtcgt cgatcacacc
      721 gtcggcgacg tcgacgccga gcgcggcctg cgcccggagg acggcgagcc acagccgccg
      781 ctccaggatg atcttgtgct cggctgacca gatctcgacc atcgcgccgc tggcgtagcg
      841 ggcggcgagg acgttgggga cggctgcttt cacacctcga ccgtactcgg cacgcttcgt
      901 ggccgagatc gcttgattcg cgaccagaac ctgccgatag aggcaaggcg acgttctgga
      961 ggggtcacgt gacgcactgc atgcgacatt tcgaagagcc ggttgtcgcc cactgccgga
     1021 cctgcgcccg gccgtactgc agccgctgcc tcgtctactc gttcggcccg gacaagccgc
     1081 cgttctgcgt gggctgcgcg ctcaacgcgt cgggcgtccg gaacaagaac atgccggtcc
     1141 cggtggccgc ccaagccgcg cctggaccgg accgcaagac ggagcgggcc cagcgcaagg
     1201 cggagaaggc cgaggccaag gcatcagccc gtgcgctcaa gcgcgccgcc aagcgcggcg
     1261 aggtcaccgc cgaagctgcc gtgcctcgca gcagcaacgt gccggtgccg aaggggctgc
     1321 cgacgccgtc cgcccggttc gccccccaga ccgagcacgc cgtcaactga ccgaggcgcg
     1381 cgcctcccgg tcggccacga gcttggcctc gaggtcggca tcgctgatcg cgagcatctg
     1441 caccacgagc agcgcggcgt tgacggagcc gtcgacggcg accgtggcga ccgggatgcc
     1501 cttgggcatc tggacggtgg cgtagagggc gtcgacgccg ttcagggcac cgcccgacag
     1561 cggcacgccg atgacgggga gggtcgtgtg ggccgcggcg gcgccggcca ggtgggcggc
     1621 catgccagca ccggcgatga tcgcggcgta gccctcggcg cgggccgacc cgacgaacgc
     1681 cgcgaccttg gcgggcgtcc ggtgcgccga catgaccgcg acctcggcct cgatgccgaa
     1741 cccggcaagg gtctccgcag cgggctccat cttggaacgg tcgttgggcg atcccatgat
     1801 gatggcgacc ttgtactggc tcacgttggc tcctcggacg aaaaggggac ggcgcgaacc
     1861 tagtgccgcg accggcaccg ccggcacggt cagccgcccg atccggcggc gccgccgccc
     1921 ggtcgatctg tcggcccgcg gcggacgcct ccggcggatg ctcctgtggt cagccgacgt
     1981 ggtcggcgta ggcctgggac gagcggacgc cgtcgatgac ggtgttgagc gaaccacccc
     2041 cggcgagcca ctcggtggcg ggttgggcct cagccactga cggaggccgg ccgagcaagg
     2101 ctgcggtgac gagggtcgga ccgacctgcg gcgccagctt ccgcttgccc tcgctcgact
     2161 ccgagaacgt cgtcatgacc cggccacggg tccacccggc gtcgagccgc gccacccagt
     2221 aggcgaggcc cggggcatcg gggctgcggc ccagcacgct gctgtagacg aaccggacga
     2281 acgccctgtt gtccagcgaa ccggacttcc ggatgaactc ggaggaaccg gcgaaggtcg
     2341 acgagatgcg atcgagcgtg cggccgttcg cccgctgctc cgaccagaac cggagtcccg
     2401 acgagtccgg cggccggccg aagtacgcgg cgtagagccg gatcagcggc tcgacctggg
     2461 tgggccaccg gcctcggtcg aggcgatcgg cgatcacgtc cgcgggcgag gccccggcct
     2521 ggaggcgggc gaggtaccac gtgagctcgt cggccgtggg cgcccgcccg tcgaccacct
     2581 ggaggatgcc ctcgacctgc tccgtcggag acgcataggg ccagttgacg gtggggcgcg
     2641 gggtcaagaa cggcgtgagg ggcgtccggg ccccggtacc cgccccgttg cgggcggcga
     2701 gcgccacgcg gaccgggacg ccctccgcga ggtcgaaggt caccttgccg gtgggtcgcg
     2761 cgaccgagcc ctgggtcggc accccgcggc ccttctcgaa caccgaggtc accagccagc
     2821 gcaccggcgg cgtgttcgga ccagccgaag ccgtcggcgg ggcgagggcg acgggcgcgt
     2881 cgatcttgtt gcccgccgcg agggcgaccg tgcccgcctt gggtgcgttc gggagcgtgg
     2941 tggaccagta gttcgtgtgg tgcttgaggg tgcggacctc gacccggttc gacacggccg
     3001 tcttcccgcc gctcgtggcg cggaaccggt aggtgcggtc caagaaggcc ccggcgttgg
     3061 tcgtgcagcg gaccacgacc gacgcgccgc gagccagcgt cccgagcgag cggccgcagt
     3121 tggtggccgg tccggacagg gagacgccg
//
LOCUS       DATMFS010000653         6347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_45979, whole genome shotgun sequence.
ACCESSION   DATMFS010000653 DATMFS010000000
VERSION     DATMFS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45979"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VNQ33_12515"
     CDS             <1..422
                     /locus_tag="VNQ33_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98978.1"
                     /translation="RVDEIVERLPDGWDTPVGEGGVALSGGERQRVGIARALLKEAAL
                     LLVDEATSALDSENERAVAAALDGGRGRRTTIVVAHRMRTIDQADHIVFIEEGRAVEA
                     GPRAELLASGGRFAEFHRDRAATGTWTIGAPDGAGDP"
     gene            complement(597..1829)
                     /locus_tag="VNQ33_12520"
     CDS             complement(597..1829)
                     /locus_tag="VNQ33_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWJ98979.1"
                     /translation="MTPAEVNVRILLAMGIVVLAARLAGWAISKIGQPRVHGEILAGI
                     LLGPSLLGIVWASGMDYAFPPDVVNGLKVIAQLGLVLFMFLIGLELDLSHLRGHGHRA
                     VVISHASIIVPIVLGAALGLWLHPRLGGDASRTGFVLFMGAAMAITAFPVLARVLQET
                     GLTHTRIGVLTITCAAVDDVTAWCVLAVIVAIVRSSGAADALVVIGWSLVYVAVMLTV
                     LRRLLQRVGVVPPWAIVAIAFLSAWVTEVIGIHAIFGAFLAGAVMPRNEATRDLAKGL
                     EMPTLTFLLPVFFIVVGLATRVDLLDDWYLWGITGLITLTAIAGKWGGAMIAARLVGE
                     HWRDAAAIGILMNTRGLSELVILTVGLELGVIGPSIFTAMVLMALVTTLMATPLLALV
                     SPDYHRGQTEAPAEPVTV"
     gene            complement(1880..2185)
                     /locus_tag="VNQ33_12525"
     CDS             complement(1880..2185)
                     /locus_tag="VNQ33_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWJ98980.1"
                     /translation="MGATAGRHEWAFITSHFLVLLCLAEDSEARMSDVAERLDLTERR
                     VQAIAAELLEAGYLEKIRVGRRNRYLINREMPLRHLQTQHRRLGDLLDLISDDEGSD"
     gene            complement(2259..4922)
                     /locus_tag="VNQ33_12530"
     CDS             complement(2259..4922)
                     /locus_tag="VNQ33_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ98981.1"
                     /translation="MDVEGVQQPGGTWPFVGRAAELEQGRRAIASQQGVVVFGGGGVG
                     KSRLAVELLGADRGRWHQIVGSAGVATVAFGAWSQVLPRSWDGAVDDAAAWRALAEHL
                     QAPDGSIHLAVDDAHWLDAGSAALLHHLVASYGATAVVTSRRDAPALQPVTALWKDGH
                     LARIDLEPLPEATVAAVVEAALGGPVEPSTIARLHARTGGSPLVIRELVDDALRAGSL
                     QLMHGAWIETTPTRPSPRLVDLLGDRIDGLAPEQRAAAEVLAVAAPLERQLATRVAPA
                     AAIQGLVDEGLAHVQRDGLRSTVALADPIMGEVLLERLDAGRRDDILGRVVDQLGQSP
                     DLDDDDVVRLVAWQIDLGRAIDPSTALHAADVADGRSDFTTAERLAAAAEAAGAGAPA
                     TIRVGEALAHQQRQAEADDVLRPIGEQLEDLDDDLRFRYGEARAFALARDLGRLDDAI
                     EVLEKTIETMADDRSRWRLEAHLSFLLADCGRLAAASPLAEARLANIDQDEVSALSAF
                     VACGLIRTYSGRCQDTLDLCERMMPIALRHLDERPEAISWIVAAQMLATYVRGDFGAT
                     LQLIEGMQLLTADEPDPTVRAGLLMAHGLLLSEQGRLETALRLLRQAAALHEVDNRRG
                     YQAWCFAITSRVHALRGDLPAAQESLAAARRRLWPGGQSFAGDIDVAAIWVATLSGDR
                     AEANRILDEGLDRAEREGMTTRALRLRHEAIRAGLPVEPHVEAFATTDITEQSLWARA
                     EVSHVAALADDDGAALVAAGNELAGIGLHLEAAESYAQGAGAHRRAGSSALAAQAKQL
                     GEAQRARCESAATPALRFGELVVGLTDRELDVTTRAAAGQTNQEIAAALGISVRTAET
                     HLQRAFAKLGIHRRGELAAVFGPPGPPGLAM"
     gene            5074..6042
                     /locus_tag="VNQ33_12535"
     CDS             5074..6042
                     /locus_tag="VNQ33_12535"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98982.1"
                     /translation="MSAKRKVDEVREGVELTNLDGPLFDGADATKRDLLDYLDAVSDR
                     LIAEVRGRPLSVIRVHRGQDAFMQKNVPTYAPGFVKTVGFWAEASKREVQYALCDDRP
                     TLLWFGNQRAVEYHPTLVLAADPGHPTHLVLDLDPPEGDGFAAAVQGARLVREVLDAA
                     GLACVVKTSGAKGVHVFVPVSGIAVDDAAAATRAIAARAEAIAPDVATTAFVKEERGG
                     KVFIDATRVGSATVAAAYSPRVRPGAPVSFPVRWDDLDSLAPGDVTITSVLDRIDDAG
                     PWLGDITSEQAVPADLVEEGHRITPGRVEAMHEGKRRKRAARGGGA"
BASE COUNT          885 a         2217 c         2424 g          821 t
ORIGIN      
        1 cccgggtgga cgagatcgtg gagcgcctcc ccgacggatg ggacacgccg gtcggcgaag
       61 ggggagtcgc cctctccggt ggcgaacgcc agcgggtcgg catcgcccgg gcgctgctca
      121 aggaggccgc cctgctcctc gtcgacgagg ccaccagcgc actcgactcg gagaacgagc
      181 gcgccgtcgc cgccgccctc gacgggggcc gcggccgacg cacgaccatc gtggtggcgc
      241 accggatgcg caccatcgac caggcggacc acatcgtgtt catcgaggag ggccgtgcgg
      301 tcgaggccgg cccccgtgcg gagctgctgg cgagcggcgg ccgcttcgcc gagttccacc
      361 gcgaccgcgc cgcgaccggc acctggacga tcggcgctcc cgatggcgcg ggggacccgt
      421 aggcccgccg tcgccggcgc gctcggcgcc ttgtcgaggg atcgagggat cgagggatcg
      481 agggatcgag gtccaggatc gttgccgcgc tcgtgcacca cgcacacgat ccgggatggt
      541 gccggcgctg accggcgggt cgacggacga ggcgagcggc cgggcgcccg ccgcggtcag
      601 acggtgacgg gttcggcggg cgcttcggtc tggccgcggt ggtagtcggg cgagacgagg
      661 gcgaggagcg gtgttgccat cagggtggtg acgagggcca tcagcaccat ggcggtgaag
      721 atgctcgggc cgatcacgcc cagctccagg ccgacggtga ggatgaccag ctcgctcagg
      781 ccgcgggtgt tcatcaagat cccgatcgcc gccgcatcgc gccagtgctc gcccaccagc
      841 cgggcggcga tcatcgcccc gccccacttg ccggcgatcg cggtcaaggt gatgagcccg
      901 gtgatgcccc agaggtacca gtcgtcgagc aggtcgacgc gggtggccaa ccccacgacg
      961 atgaagaaca cgggcaggag gaacgtgagc gtcggcatct cgagcccctt cgcgaggtcc
     1021 cgggtggcct cgttgcgggg catgaccgcg ccggccagga acgcaccgaa gatggcgtgg
     1081 atgccgatca cctcggtgac ccaggcgctc aagaaggcga tggcgacgat ggcccagggc
     1141 ggtacgacgc cgacgcgctg gaggaggcgg cgcaggaccg tgagcatgac ggcgacgtag
     1201 accagcgacc agccgatcac cacgagggcg tcggcggcgc ccgatgacct cacgatggcg
     1261 acgatgacgg cgaggacgca ccacgccgtg acgtcgtcga cggcggcgca ggtgatggtc
     1321 aggaccccga tccgggtgtg ggtgagcccg gtctcctgga gcacgcgggc gaggacgggg
     1381 aacgcggtga tggccatggc cgcgcccatg aagagcacga agccggtgcg gctggcgtcc
     1441 ccgccgagcc gcgggtggag ccagagcccc agcgctgctc ccagcacgat ggggacgatg
     1501 atcgaggcgt ggctgatgac gaccgctcgg tgtccgtggc cccgcaggtg gctgaggtcc
     1561 agctcgaggc cgatcaggaa catgaacagc acgaggccca gctgggcgat caccttgagg
     1621 ccgttcacga cgtcgggcgg gaaggcgtag tccatgcccg acgcccagac gatgccgagc
     1681 aggctgggcc cgaggaggat cccggcgagg atctcgccgt gcacgcgcgg ctggccgatc
     1741 ttggagatcg cccaccccgc gagccgcgcc gccagcacga cgatgcccat ggcgagcagg
     1801 atccggacgt tgacctcggc tggtgtcacg caccctcccc caaaggcaga actctgattc
     1861 acgaaacata gttcgtgcgt cagtcggagc cctcatcatc ggagatcagg tcgagcagat
     1921 cgccgagccg gcggtgctgg gtctggagat ggcgcagcgg catctcccgg ttgatcaagt
     1981 agcggttgcg gcggccgacg cggatcttct cgaggtagcc cgcctcgagc agctcggcgg
     2041 cgatcgcctg gacgcgccgt tcggtgaggt cgaggcgctc ggccacgtcg gacatgcggg
     2101 cctcggagtc ctcggcgagg cacagcagga cgaggaagtg gctggtgatg aacgcccatt
     2161 cgtgtcgacc ggccgttgct cccacgggcc gcaaccgtac cggccggtcc cgcgggcgct
     2221 gcccgtcccg ggccggggtg cctcgccgtc ggtggggact acatcgcaag tccgggaggg
     2281 cctggtggac cgaacacggc cgccagctcg ccgcggcggt ggatgcccag cttggcgaag
     2341 gcccgctgga ggtgggtctc ggcggtgcgg accgagatgc ccagcgccgc cgcgatctcc
     2401 tggttcgtct gaccggcggc ggcccgggtg gtgacgtcga gctcccggtc ggtgaggccc
     2461 accaccagct ccccgaaccg cagcgccggg gtggcggccg actcgcagcg cgcccgctgg
     2521 gcttcgccca gctgcttggc ctgggccgcc agggccgagg atccggcgcg gcggtgggcg
     2581 ccggccccct gggcgtagga ctcggccgcc tccaggtgga ggccgatgcc ggcgagctcg
     2641 ttgccggcgg cgacgagggc ggcgccgtcg tcgtcggcga gcgcggccac gtggctgacc
     2701 tcggcccggg cccagaggct ctgctcggtg atgtcggtgg tggcgaacgc ctcgacgtgc
     2761 ggttccaccg ggaggccggc tcggatggcc tcgtggcgga ggcgcagcgc ccgcgtggtc
     2821 atgccctcgc gctcggcccg gtccaggccc tcgtccagga tccggttcgc ctcggcccga
     2881 tccccggaca aggtggcgac ccagatggcc gccacgtcga tgtcgccggc gaaggactgc
     2941 ccgccgggcc agaggcggcg gcgggccgcg gccagcgact cctgggcggc aggcaggtcg
     3001 ccgcgcaggg cgtggacccg cgacgtgatg gcgaagcacc aagcctggta gccgcgccgg
     3061 ttgtccacct cgtggagggc ggcggcctgg cgcagcagtc ggagggcggt ctcgagccgg
     3121 ccctgctcgg agagcaggag cccgtgtgcc atcagcagcc cggcgcggac cgtcggatcc
     3181 ggctcgtcgg cggtgaggag ctgcattccc tcgatcagct gcagcgtcgc gccgaagtca
     3241 ccccgcacgt aggtggccag catctgggcg gcgacgatcc agctgatcgc ctcgggccgc
     3301 tcgtcgaggt ggcggagggc gatcggcatc atccgctcgc acaggtcgag cgtgtcctgg
     3361 caccgtccgc tgtaggtgcg gatcaggccg caggcgacga aggcgctgag cgccgagacc
     3421 tcgtcctggt cgatgttcgc caggcgggcc tcggccaagg gggaggcggc ggcgagccgt
     3481 ccgcagtcgg cgaggaggaa cgacaggtgc gcctcgagcc gccagcggga ccggtcgtcg
     3541 gccatcgtct cgatggtctt ctccagcacc tcgatggcgt cgtcgagccg gcccaggtca
     3601 cgggcgagag cgaaggcccg ggcttcgccg tagcggaacc gcaggtcgtc gtcgaggtcc
     3661 tcgagctgct cgccgatcgg acggagcacg tcgtcggcct cggcctggcg ctgctggtgg
     3721 gcgagcgcct cgcccacccg gatggtggcg ggcgcgccgg cgccggcggc ctcggccgcc
     3781 gccgccaagc gctcggcggt ggtgaagtcg ctgcgcccgt cggccacgtc cgcggcgtgc
     3841 agggcagtac tcggatcgat ggcccggccg aggtcgatct gccaggcgac gagccgcacc
     3901 acgtcgtcgt catcgaggtc cgggctctgg ccgagctggt cgaccacgcg cccgaggatg
     3961 tcgtcgcggc gtcccgcgtc gagccgctcg aggaggacct cgcccatgat cgggtcggcc
     4021 agggcgacgg tgctgcggag cccgtctcgc tggacgtggg ccagaccctc gtccaccagg
     4081 ccctggatcg ccgcggccgg tgcgacccgg gtggcgagct ggcgttcgag cggtgccgcc
     4141 accgccagca cctcggccgc ggcccgctgc tcgggagcga ggccgtcgat gcggtcgccg
     4201 agcaggtcga ccagccgggg gctcggtcgc gtcggcgtgg tctcgatcca ggcgccgtgc
     4261 atcagctgga gcgatccggc acggagggcg tcgtcgacca gctcgcggat cacgaggggg
     4321 ctgccgccgg tccgggcatg cagccgggcg atggtgctgg gctccaccgg tccgccgagg
     4381 gctgcttcga ccaccgcggc gacggtcgcc tccggcaggg gctcgaggtc gatgcgagcg
     4441 aggtgtccgt ccttccacag cgcggtgacg ggctggaggg cgggtgcgtc ccggcggctg
     4501 gtcacgacgg cggtggcgcc gtagctggcg acgaggtggt ggaggagcgc ggccgatccg
     4561 gcgtcgagcc agtgggcgtc gtccacggcc aggtggatcg acccgtcagg agcctgcagg
     4621 tgctcggcca gcgccctcca ggccgcagcg tcgtcgacgg caccgtccca cgaccggggg
     4681 agcacctgcg accaggcccc gaacgcgacg gtggcgacac cggccgatcc gacgatctgg
     4741 tgccaccggc cccggtcggc tccgagcagc tcgacggcca gccgggactt gcccactccg
     4801 cccccgccga agaccacgac gccctgctgc gatgcgatgg cccggcgccc ctgctccagc
     4861 tcggcggcgc gcccgacgaa gggccacgtc cctcccggtt gctgcacccc ttcgacgtcc
     4921 atctcccgca acctcccgcc cgtccatgat ggcgcgccgc gtcgttcggc gggaccgggg
     4981 ccgatccgaa cgccccgccg cgaccggccg gttcccgcct gccggttccg gcatcagctg
     5041 cgccacgttc gctcgtcggg gtagaccagc ggggtgagcg cgaagcggaa ggtcgacgag
     5101 gtgcgcgagg gggtggagct caccaacctc gacgggcccc tgttcgacgg cgccgatgcc
     5161 accaagcggg acctcctcga ctacctcgac gccgtgagcg accgcctgat cgccgaggtg
     5221 cgcggccggc cgctgtcggt catccgggtg caccggggcc aggacgcgtt catgcagaag
     5281 aacgtgccga cgtacgcgcc cggcttcgtg aagacggtcg ggttctgggc cgaggcgtcc
     5341 aagcgcgagg tgcagtacgc cctctgcgac gaccggccga cgctgctgtg gttcggcaac
     5401 cagcgggccg tcgagtacca cccgacgctc gtgctggccg ccgacccggg gcacccgacc
     5461 cacctggtgc tggacctcga cccacccgag ggcgacggct tcgcagcggc ggtccagggc
     5521 gcccgcctcg tgcgggaggt gctcgacgcc gccggcttgg cctgcgtggt gaagacgagc
     5581 ggggccaagg gcgtgcacgt gttcgtcccc gtgtccggca tcgccgtcga cgatgcggcc
     5641 gccgccaccc gtgccatcgc cgctcgggcc gaagccatcg ccccggacgt ggcgaccacc
     5701 gccttcgtga aggaggagcg cggcggcaag gtgttcatcg acgccacccg ggtgggcagc
     5761 gccacggtgg ccgcggccta cagcccccgg gtgcggccgg gcgccccggt ctcgttcccg
     5821 gtccgctggg acgacctcga ctcgctggcc ccgggcgacg tcaccatcac ctccgtgctc
     5881 gaccgcatcg atgacgccgg gccgtggctc ggcgacatca cgtcggagca ggcggtcccg
     5941 gccgatctgg tcgaggaggg ccaccggatc acgcccgggc gggtcgaggc catgcacgag
     6001 ggcaagcgcc gcaagcgggc ggcgcggggc gggggcgcct gagcccgcgg gcggcaccgg
     6061 cgggcggatg agcggatggg gcggaagatc cgtgtgcgtc gcggacccga acccacccgt
     6121 ttggggaggt tggggaggga atccgaccga acatcggcaa aatcccacct ttcgtggttg
     6181 agatcacacc cagggtggcc gacaagaagg gtgtggaggg ctccatcacc acgcgcggca
     6241 gggctcccga ggccctgtcg ctcgaacagc acctcgggga cggggcggat ttcgacgcgc
     6301 ccgaccgctt cgacctgttc atcaactggc tgacgtcgtc catccag
//
LOCUS       DATMFS010000654         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_92178, whole genome shotgun sequence.
ACCESSION   DATMFS010000654 DATMFS010000000
VERSION     DATMFS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92178"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNQ33_12540"
     CDS             complement(<1..524)
                     /locus_tag="VNQ33_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossman fold protein, TIGR00730 family"
                     /protein_id="HWJ98983.1"
                     /translation="MTDQHTDPAASSIAAEAASFLDHLGIVTNRDLISEIVTTAGELG
                     QDRTDRLDLKITASAIDEMRDAFLMFAPYKDRPKVTIFGSARTAAVDPVYVQTHDVAS
                     ALAGAGWMVVTGAGPGIMLAAMEGAGPAQSIGVSIRLPFESRANDVIHDDPKHVSMKY
                     FFTRKLMLVKESKG"
     gene            complement(521..>2536)
                     /gene="cofH"
                     /locus_tag="VNQ33_12545"
     CDS             complement(521..>2536)
                     /gene="cofH"
                     /locus_tag="VNQ33_12545"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HWJ98984.1"
                     /translation="STVDYLVAMAGLVVSETGLLPHANAGALYADELEALRAVSPSQG
                     MMLESLRGDLDAHRGSPDKEPARRLATLETAGELHIPFTTGILVGFGEDRADRIEALE
                     AIAASHARWGHVQEVIVQNFLPKPGTGMHAAPPCPPDTYLEAIAVARLILPPEIHLQA
                     PPNLSDDFGVLLDAGIDDWGGVSPVTADHVNPERPWPDRDRLRAVTEDAGFELAPRLT
                     IYPEFATQPETWLHEDLRFPVLDRSDAEGLGRDDPGAVHPQTVGEVRNVGDGAEVVLV
                     GRRSTQWYVGLDADPPVLVPGPARATGAVADVLAAVRLGDVPDEDQITTLFSARGREV
                     GAVVELADELRQQVVGDAVTWVSNRNINYTNVCTFKCQFCGFSKGPRSLNLRGTPYLL
                     TLDDIAQRAVEAQEMGATEVCLQGGIHPDFDGDYYIDVARAVKDAVPDMHVHGFTALE
                     VTEGAKRLGEPLADYLRRLVGVGLATLPGTAAEILDDEVRAVLCPDKVNTQEWLDAHR
                     TAHSVGLRSNVTIMFGSIEHPRSWARHLVRTRALQAETGGFTEFVPLPFVHMASPIYL
                     RKRARRGPTWREVVLMHAVGRIAYHGFIPNIQASWVKLGVAGVQQLLQAGVNDLGGTL
                     IDENISRAAGASHGQGMDEDAFRAVVEPLGRRLEQRTTLYGSAPQPA"
BASE COUNT          345 a          813 c         1002 g          376 t
ORIGIN      
        1 aagcccttgg actccttcac cagcatcagc ttgcgggtga agaagtactt catcgacacg
       61 tgcttggggt cgtcgtggat gacgtcgttg gcccggctct cgaacgggag ccggatgctg
      121 acgccgatcg actgcgccgg accggccccc tccatggcgg cgagcatgat cccgggtcca
      181 gctccggtga ccaccatcca gccggcgccg gccagggcgg acgcgacgtc gtgggtctgc
      241 acgtagaccg gatcgacggc ggcggtgcgg gccgacccga agatggtgac cttgggccgg
      301 tccttgtacg gcgcgaacat caagaaggcg tcgcgcatct cgtcgatggc cgatgccgtg
      361 atcttgaggt ccaggcggtc ggtgcggtcc tggcccagct cgcccgcggt ggtcacgatc
      421 tcgctgatca ggtcccggtt ggtgacgatg ccgaggtggt cgaggaagga ggccgcttcc
      481 gcggcgatgg aggatgcagc agggtcggtg tgctggtcgg tcatgcgggc tggggtgccg
      541 agccgtagag cgtggtccgc tgctccagtc ggcggccgag gggctcgacg acggcgcgga
      601 aggcgtcctc gtccatgccc tggccgtggc tcgcgccggc ggcgcgggag atgttctcgt
      661 cgatgagcgt gccgcccagg tcgttgacgc cggcctggag cagctgctgc acgccggcca
      721 ccccgagctt cacccaggag gcctggatgt tcgggatgaa gccgtggtag gcgatgcggc
      781 ccacggcgtg catcagcacg acctcgcgcc aggtgggccc gcggcgggct cgcttgcgca
      841 ggtagatggg cgaggccatg tgcacgaacg gcagggggac gaactcggtg aagccgccgg
      901 tctcggcctg gagggcgcgg gtgcgcacca ggtgccgggc ccaggaacgg gggtgctcga
      961 tggagccgaa catgatcgtg acgttggagc gcaggcccac cgagtgggcg gtgcggtggg
     1021 cgtcgagcca ctcctgcgtg ttcaccttgt cggggcacaa gacggcccgg acctcgtcat
     1081 cgaggatctc ggccgccgtg cccggcaggg tggcgaggcc cacgccgacg agccggcgca
     1141 agtagtcggc caggggctcg cccaggcgct tggcgccctc ggtgacctcg agggcggtga
     1201 acccgtggac gtgcatgtcg ggcacggcgt ccttcacggc ccgggcgacg tcgatgtagt
     1261 agtcgccgtc gaagtccggg tggatgccgc cctggaggca cacctcggtg gctcccatct
     1321 cctgggcctc gacggcgcgc tgggcgatgt cgtcgagcgt gagcaggtac ggcgtgcccc
     1381 ggaggttgag cgacctcggg cccttcgaga acccgcagaa ctggcacttg aaggtgcaga
     1441 cgttcgtgta gttgatgttg cggttggaca cccaggtgac ggcgtcgccg acgacctgct
     1501 ggcgcagctc gtcggccagc tccaccactg caccgacctc gcggccgcgg gccgagaaca
     1561 gggtggtgat ctggtcctcg tcggggacgt cgccgaggcg cacggccgcc agcacgtcgg
     1621 ccaccgctcc cgtggcccga gcggggccgg gcaccaggac cggcgggtcg gcgtcgaggc
     1681 ccacgtacca ctgcgtcgac cggcggccga ccagcacgac ctcggcgccg tcgcccacgt
     1741 tgcggacctc gcccacggtc tgcgggtgga cggccccagg gtcgtcgcgt ccgaggcctt
     1801 cggcatcgga gcggtcgagc accgggaacc ggaggtcctc gtggagccag gtctcgggct
     1861 gggtggcgaa ctcgggatag atggtgagcc gcggcgccag ctcgaagccg gcgtcctcgg
     1921 tgacggctcg gagccggtcg cggtcgggcc aggggcgctc ggggttcacg tggtcggcgg
     1981 tgacggggga gacgccgccc cagtcgtcga tgccggcatc gagcagcacg ccgaagtcgt
     2041 cggacaggtt cggcggggcc tggaggtgga tctccggcgg gaggatgagg cgggcgacgg
     2101 cgatggcctc gaggtaggtg tcgggcgggc agggcggcgc cgcgtgcatg ccggtgccgg
     2161 gcttgggcag gaagttctgg acgatcacct cctgcacgtg gccccagcgg gcgtggctgg
     2221 cggcgatggc ctccagcgcc tcgatgcggt cggctcggtc ctcgccgaac ccgacgagga
     2281 tcccggtggt gaacgggatg tgcagctcgc cggcggtctc gagcgtggcc aggcggcgag
     2341 ccggctcctt gtcggggctg ccccgatggg cgtcgaggtc gccccggagg gactcgagca
     2401 tcatgccctg cgacggggag accgctcgca gggcctccag ctcgtcggcg tagagggcgc
     2461 cggcgttggc gtgggggagc aggccggtct ccgacacgac caggccggcc atggcgacca
     2521 ggtagtccac cgtgga
//
LOCUS       DATMFS010000655         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_68490, whole genome shotgun sequence.
ACCESSION   DATMFS010000655 DATMFS010000000
VERSION     DATMFS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68490"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..95)
                     /locus_tag="VNQ33_12550"
     tRNA            complement(19..95)
                     /locus_tag="VNQ33_12550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:Arg,seq:acg)
     gene            209..781
                     /locus_tag="VNQ33_12555"
     CDS             209..781
                     /locus_tag="VNQ33_12555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWJ98985.1"
                     /translation="MHVVGVSGSLQARSNTAAVLASVADLVTLRADLEAPPGAACATA
                     PTFEVFTGIEELPHFNPDRDVDPPHPAVATWRSTLEEADAVVIATPEYAHGYPGALKD
                     ALDWIVGSGELVDTPVLLITASGGGGVRAGEALSPVLSVMAGHLVGPISIWGVAPKVA
                     EGRLTDPDDLDALAAAVELLLSLAAAPAEA"
     gene            841..1581
                     /locus_tag="VNQ33_12560"
     CDS             841..1581
                     /locus_tag="VNQ33_12560"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HWJ98986.1"
                     /translation="MHHVPIPDQMAERISALLDKAESSSFPEEADAFMAKAQELMARH
                     AIDEAMLDAARGSNEEIAQVEVTIVAPYASAKSVLLNAVAASNRCRLVVSNRPGGRVL
                     CSLVGFETDIRHTRSLYLSLSHQAVRFMLGAPLPPGDTHRRFRHSFLLAYAWRIGERL
                     EEADALARTEAEAEQLERPDGGSVSLVMASREAKVDRAFAEAFPRLGTKRISSSSGGG
                     HVMGRAAADRSSLDRPSVAGGGRSLSSG"
     gene            complement(1715..>2051)
                     /locus_tag="VNQ33_12565"
     CDS             complement(1715..>2051)
                     /locus_tag="VNQ33_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ98987.1"
                     /translation="DAYLVTVRDTRPHVVAVSVVHRDEAIVVGAGRRTAVNVVDRPDA
                     TLLWPVSPEHPKHSLLVDGTARVSDDGEWLIITPTSAILHRAGGRRRALAHDETAPAA
                     PVPPDDAEA"
BASE COUNT          286 a          736 c          728 g          301 t
ORIGIN      
        1 ctgacctgcg tatttctctg gcgcgcttgc agggactcga accccgaacc ttctgatccg
       61 tagtcagatg atctatccaa ttgagctaca agcgcagagc gctgggcgcc ggacgaccct
      121 agtcgggcgc ccacggccgc atccaacgac atgagcggcc gccgacctgg gcgttggcag
      181 gtcctgggat caggggtaca accaacctgt gcacgtcgtc ggggtgtcgg ggagcttgca
      241 ggctcggtcg aacacggccg cggtgctcgc ctcggtcgcc gatctggtga cgctgcgggc
      301 tgatctcgaa gcgcctcccg gcgcagcgtg cgcgacggcg ccgacgttcg aggtgttcac
      361 cgggatcgag gagctgccgc acttcaaccc ggaccgtgac gtcgacccgc cgcatccggc
      421 cgtcgccacc tggcgatcga cgctggagga ggccgacgcc gtcgtcatcg ccacgccgga
      481 gtacgcccac ggctacccgg gtgcgctgaa agatgcgctc gactggatcg tcgggtcggg
      541 ggagctggtc gacacgcccg tgctgctgat caccgcatcg ggcggcggtg gagtccgggc
      601 aggcgaggcg ctatcgcccg tgctgtcggt catggccggg cacctcgtcg ggcccatcag
      661 catctggggc gtcgcaccga aggtcgccga gggccgcctc accgaccccg acgacctcga
      721 cgcgctggcc gccgccgtcg agctgctcct ctccctcgct gccgcccctg ctgaggcctg
      781 actgtcagtc cccctggtca cgatggttcg gtcatcgggg ggatagaacc ggaggcagcc
      841 atgcaccacg taccgatccc cgaccagatg gccgagcgca tcagcgccct gctcgacaag
      901 gccgagtcgt cctcgttccc cgaggaggcc gacgccttca tggccaaggc ccaggagctg
      961 atggcccgcc acgccatcga cgaggcgatg ctcgacgccg ctcgggggtc gaacgaggag
     1021 atcgcccagg tcgaggtgac gatcgtcgcg ccctacgcat cggcgaagtc ggtgctgctc
     1081 aacgcggtgg cggcgtccaa ccgctgccgg ctcgtcgtct cgaaccggcc gggcggtcga
     1141 gtgctgtgct cgctcgtcgg gttcgagacc gatatccgcc acacccgctc cctctacctc
     1201 tcgctctcgc accaggccgt gcggttcatg ctcggtgcgc cgctcccacc gggcgacacg
     1261 caccgacgct tccgccactc gttcctcctg gcctacgcgt ggcggatcgg tgagcggctc
     1321 gaggaggccg acgcgctcgc ccggaccgaa gcggaagccg agcagctgga gcgccccgac
     1381 ggcgggtcgg tctcgctggt gatggccagc cgcgaggcga aggtcgaccg cgccttcgcc
     1441 gaggcgttcc cccggctcgg caccaagcgc atcagcagct ccagcggcgg cgggcacgtg
     1501 atgggccgag ctgccgccga ccgctcatcg ctcgaccggc cgtccgtcgc cggcggtggt
     1561 cgttcgctct ccagcggctg atcgacggcg ggcagcgctc accgggccgg gttgtcgctc
     1621 cgacgtgacc caacaggaca ccgcagcatc ggacgggtcc gggaccaggc cgaacaggcc
     1681 gcgacctccc caccggtgtc accgagtcgg gtggtcaggc ttcggcgtcg tccggcggga
     1741 ccggcgcggc cggcgcggtc tcgtcgtggg cgagggctcg gcggcggccg ccggcccggt
     1801 ggaggatggc gctggtcggg gtgatgatca gccactcacc gtcgtcggac acccgggcgg
     1861 tgccgtccac caggaggctg tgcttcgggt gctcgggcga caccggccac aggagggtgg
     1921 catcgggacg atcgaccacg ttcaccgcgg tgcggcggcc ggcgccgacg acgatcgcct
     1981 cgtcccggtg caccaccgac acggcgacga cgtgcggccg ggtgtcgcgg acggtcacca
     2041 ggtaggcgtc c
//
LOCUS       DATMFS010000656         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_68491, whole genome shotgun sequence.
ACCESSION   DATMFS010000656 DATMFS010000000
VERSION     DATMFS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68491"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNQ33_12570"
     CDS             complement(<1..312)
                     /locus_tag="VNQ33_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ98988.1"
                     /translation="MTKSFAGADHPAVAPLDLTVNAGEITVLIGPSGCGKTTTLRMIN
                     RLVNPTDGVITIDGTDIRQRSLTDLRRAIGYVIQQIGLFPHRTIAQNIATVPKLLGWD
                     RA"
     gene            complement(899..1933)
                     /locus_tag="VNQ33_12575"
     CDS             complement(899..1933)
                     /locus_tag="VNQ33_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWJ98989.1"
                     /translation="MDPLEALLRVAYLQDRGLLPSQKTAAFLKAADIVRELPEGELER
                     RVASRTLTDLPGIGASTSEVITQALAGKVPDRIAKLEAETAIPIGPGASLRAAIKGDC
                     HAHSTWSDGGASVETMARAAQSLGHEYLVMTDHSPRLTVAHGLDRDRLLAQLDEIAAL
                     NDELDGFRILTGIEVDILVDGGLDQDEDILDRLDIVVASVHNKLSMDPVPMTERILKA
                     VASPHVDILGHCTGRKVKGWGDDLREIRPRSTYDEQLIFDACAVYGTAVEINCRPERQ
                     DPPPELLDLALDAGCLISIDTDSHSPGHLEFVNYGCDKAVAHGIDPGRIINTWPLDDL
                     LTWTRTRVAA"
     gene            2000..2818
                     /locus_tag="VNQ33_12580"
     CDS             2000..2818
                     /locus_tag="VNQ33_12580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWJ98990.1"
                     /translation="MQFRAIATDLDGTLLRDDKSVSARTRAAVDAAEDAGLLFVIATG
                     RPPRWIPPVIEQLGERGLVVCANGGSVFDPARHELVARNDLDPTVVADLVDDVSGAFP
                     DALFGVEQGFRFAVDELVLQRPEDQARMDSWKDADLRIVPIRSVLDEGVTKLIIRLPH
                     PAPDGTADAVQAVVGRRAKVTHSVDESFLELSRPDITKAATVEQLLLESGIAPHEVVA
                     FGDMPNDLALIEWAGLGVAVANAHPALREAADEVTVSNDDDGVAVVIERILSEL"
     gene            2825..>3142
                     /locus_tag="VNQ33_12585"
     CDS             2825..>3142
                     /locus_tag="VNQ33_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98991.1"
                     /translation="MVLATGLALAAACLHATWNLILKRARHEDRDLTSWGLFLFGALL
                     VTPPMIAMGGPGLDVLPWLVLSGAVHMAYITALVGAYRHGDFSLAYPLARGGGALFAA
                     VGGA"
BASE COUNT          428 a         1043 c         1174 g          497 t
ORIGIN      
        1 cgccctgtcc cagccgagga gcttcggcac cgtggcgatg ttctgggcga tggtccggtg
       61 cgggaacaac ccgatctgct ggatgacgta gccgatggcg cggcgcaggt cggtgagcga
      121 ccgctggcgg atgtcggtgc cgtcgatggt gatgacgccg tcggtggggt tcaccaaccg
      181 gttgatcatg cgcagcgtgg tggtcttgcc gcagcccgac ggcccgatca gcacggtgat
      241 ctcgccggcg ttcaccgtga ggtcgagggg cgcgacggcc gggtggtccg ctcccgcgaa
      301 cgacttcgtc acgcctcgca gctcgatcgc cgtccccgtc atctcgtcca ctccctaccc
      361 gcgggtgcag gcgcgccaga ccacggggtt gtgaggatgc tgacggctcc accctgcccc
      421 tcgcagtgtc cggagatccg tcgcgcacgg cgctgggaag cgcgtcgctg ggtcttgatg
      481 acgccagacc ctaccccctg atcaccgaaa cccgaccgat tgcatcggaa ttgaaacgcc
      541 ccgacgatgg ggaagcccga cgaggcgagg gccccagacg cgccgaagcc cccgaacgag
      601 ccgagggcca cgaggtcgaa gcatcggttc cgagcgaagc gagcccgggg gagttcgagg
      661 gggcggagct tgcggagccc cgggttctca agccagcgag gtgcgacgaa gtcgtgccga
      721 gcggcgaagg cgccggggcg tcagcccatc ctcacgttcg aggagcggag cgagcgcagc
      781 gagcgaacga cgagaggagc gtcagcgacc cgcgttgggc ttggtgccgt ggttcgcctt
      841 cttgcggcgg gcgttggtct tgcgcttgtc cgtcttcttc gacataaggc ttgccacgct
      901 acgccgcgac gcgggtgcgg gtccaggtga ggaggtcgtc gagcggccag gtgttgatga
      961 tccggccggg gtcgatgccg tgggcgacgg ccttgtcgca gccgtagttg acgaactcca
     1021 ggtgcccggg ggagtgcgag tcggtgtcga tcgagatgag gcagccggcg tcgagcgcca
     1081 ggtccagcag ctcaggtggc gggtcctggc gctcggggcg gcagttgatc tcgacggcgg
     1141 tgccgtagac ggcgcaggcg tcgaagatga gctgctcgtc gtaggtggag cggggccgga
     1201 tctcgcgcag gtcgtcgccc cagcccttga ccttgcggcc ggtgcagtgg ccgaggatgt
     1261 cgacgtgggg gctggcgacc gccttgagga tgcgctcggt catcggcacc gggtccatcg
     1321 acagcttgtt gtggaccgag gccacgacga tgtcgaggcg atcgaggatg tcctcgtcct
     1381 ggtcgaggcc gccgtcgacg aggatgtcga cctcgatccc ggtgaggatg cggaagccgt
     1441 cgagctcgtc gttgagcgcg gcgatctcgt cgagctgggc gaggagccgg tcccggtcga
     1501 ggccgtgggc gacggtgagc cgcggcgagt ggtcggtcat cacgaggtac tcgtggccga
     1561 gcgactgggc ggcacgggcc atggtctcga cggacgcacc gccgtcgctc cacgtggagt
     1621 gggcgtggca gtcgcccttg atggccgccc ggaggctcgc accggggccg atcgggatgg
     1681 cggtctccgc ttcgagcttg gcgatgcggt ccggcacctt gccggcgagc gcttgggtga
     1741 tgacctcgct cgtactggct ccgatgccgg gcaggtccgt gagcgtgcgg ctggcgaccc
     1801 ggcgctcgag ctcgccctcg ggcagctccc gcacgatgtc ggcggccttg aggaaggcgg
     1861 cggtcttctg cgaggggagc aggccgcggt cttggaggta ggcgacgcgc agcagggctt
     1921 cgagcggatc catccctggc gagggtagcg acggaacaga ccgtgggcac ccaggtcccc
     1981 accgctacgg tcgctcctcg tgcagttccg ggcgatcgcg accgacctcg acgggacctt
     2041 gctccgcgac gacaagtcgg tgtcggcccg aacccgagca gcggtcgacg cggcagagga
     2101 cgccggcttg ctcttcgtga tcgccaccgg ccgtccgccg cggtggatcc cgccggtcat
     2161 cgagcagctc ggcgagcgcg gcctggtggt ctgcgccaac ggcggctcgg tcttcgatcc
     2221 ggcccgccac gagctcgtcg cccgcaacga cctcgacccg acggtcgtcg ccgatctggt
     2281 ggatgacgtc tccggcgcgt tccccgatgc gctcttcggc gtcgagcagg gcttccgctt
     2341 cgccgtcgac gagctcgtcc tgcagcggcc ggaggaccag gcccgcatgg acagctggaa
     2401 ggacgccgac ctgcggatcg tgcccatccg ctcggtgctc gacgagggcg tgacgaagct
     2461 catcatccgc cttccccacc ccgcgcccga tgggacggct gacgccgtgc aggcggtggt
     2521 cgggcgccgg gccaaggtca cgcactcggt cgacgagtcg ttcctggagc tgtcgcggcc
     2581 cgacatcacg aaggcggcga ccgtcgagca gctcctgttg gagtccggca tcgctccgca
     2641 cgaggtggtc gccttcggcg acatgcccaa cgacctggcg ctcatcgagt gggccggcct
     2701 cggcgtcgcc gtggccaacg cccacccggc actccgggag gcggccgacg aggtgaccgt
     2761 cagcaacgac gacgacggcg tggccgtcgt gatcgagcgc atcttgagcg agctctgacg
     2821 gcgagtggtc ctcgccaccg gcctggccct ggcggccgcc tgcctccacg ccacctggaa
     2881 cctcatcctc aagcgggcac gccacgagga ccgtgacctc acgtcgtggg ggctgttcct
     2941 gttcggcgcg ctgctggtga cgccgccgat gatcgccatg ggcgggccgg gcctcgacgt
     3001 cctgccctgg ctcgtgctgt cgggcgcggt gcacatggcg tacatcaccg ccttggtcgg
     3061 ggcgtaccgc cacggggact tctcgctcgc ctacccgctg gcacgcggcg gcggtgcgct
     3121 gttcgccgcc gtgggcgggg cg
//
LOCUS       DATMFS010000657         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_22455, whole genome shotgun sequence.
ACCESSION   DATMFS010000657 DATMFS010000000
VERSION     DATMFS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22455"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..2008
                     /locus_tag="VNQ33_12590"
     CDS             182..2008
                     /locus_tag="VNQ33_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWJ98992.1"
                     /translation="MKLVSIGAAAAALAAGCSSGSGGSDGSSSNDPFGDPGDCTVIDV
                     AVSSEKIDLMTDLAQQFNRSEDAEISGGCAFVRPYSKASGGATTALAEGWDEASEGPR
                     PVIWSPAASSWGQILDQQLADAGKPAMASPKPVSFMLTPLVIGMPKPMADALGYPTTP
                     IGWADIAQLATSGEGWAKYGHPEWGAFKLGKTNPNFSTSGLNALIGQNYAATGKTRDL
                     SKEDLDAQRTKDLGRQVESSVVHYGDITMTFLNNWFRTDRSGTSLLYASAVAVEEKSL
                     IDYNQGNPDGVLDPGETPRKPKVPLVAIYPKEGTLYSDSPLYVLDADWVSAQEKEGAE
                     AFIEAVQRPAAQKKVLEYGFRPGNPDVAVGAPITKGNGVDPTQPSKLLQVPQPSVMID
                     MLENWKQQRKGARVLLVLDVSGSMEEPADPDDPASDTKLDLAKRAAIDALDEFKGDDE
                     VGLRVFSTDLGSREDENYLDLLEIQPMSTNRERLASQIRDQLPQHATPLYEVTQDSFE
                     DMADGYDPSLINAVVLLTDGVNDDGDTSDDRDQLDELLTTLQSGTNGENATPIRVFTI
                     AYGKQADFDTLERIAESTNAAAYDASNPATISQVFTAVVSNF"
     gene            2073..>2841
                     /locus_tag="VNQ33_12595"
     CDS             2073..>2841
                     /locus_tag="VNQ33_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98993.1"
                     /translation="MSLRDRFFSPPVARALTSPSGIVALGAGAALGILATIGTGGLLG
                     PVFGGLLGGALGYGGRVALAIPRNGATASIDPFGVNEPWRHAVKDAIQARHRYTDAIG
                     AFKAGPLRDALHETGDRIDDAVNECWKVAQQGQLLSTARKRIDDRETQWELQQAAGQI
                     PPGATASPTQARTIASLNAQLDTARRMDALVASTYDELRLINARLDEAVTQAIELSVT
                     DHGDLSTVSSDVDDIVGSLTALRTAMTSLDGTGPQDLG"
BASE COUNT          485 a         1084 c          909 g          363 t
ORIGIN      
        1 ggtcacgagg acgacgtcga cgggctcggc tgcaaggatc tgcccaaggc ggcgaagacg
       61 gccctcacct cggtcgggtt cgcgccgtgc acgggatcgg ccgacgatgc cctggcaggc
      121 ttcctgtacc aagtccagaa gaaggtgggt tgagatggtg ccacagcccc gatcccggtg
      181 gatgaagctc gtcagcatcg gcgcggccgc cgccgcgctc gcggccgggt gctcgtcggg
      241 cagcgggggc tcggacggct cctcgtcgaa cgaccccttc ggcgacccgg gcgactgcac
      301 ggtcatcgac gtggcggtct cctccgagaa gatcgacctg atgaccgatc tcgcccagca
      361 gttcaaccga tcggaggatg cggagatctc cggcggctgc gccttcgtgc gcccgtactc
      421 gaaggcatcc ggcggagcca ccacggcgct ggccgaaggc tgggacgagg cgtccgaagg
      481 ccctcgaccc gtcatctggt ccccggccgc ctccagctgg ggccagatcc tcgaccagca
      541 gctggccgac gccggcaagc cggcgatggc gtcgccgaag ccggtgtcgt tcatgctcac
      601 gccgctcgtc atcggcatgc ccaagccgat ggccgacgcc ctcggctacc cgacgacccc
      661 catcggctgg gccgacatcg cccagctcgc cacctccggc gagggctggg ccaagtacgg
      721 gcaccccgag tggggcgcct tcaagctcgg caagacgaac ccgaacttct ccaccagcgg
      781 cctcaacgcc ctgatcggcc agaactacgc cgccaccggc aagacccggg acctgtcgaa
      841 ggaggacctc gacgcccaga ggacgaagga cctcggccgc caggtcgagt cctcggtggt
      901 ccactacggc gacatcacga tgacgttcct caacaactgg ttccgcaccg accggtcggg
      961 cacgtcgctg ctctacgcgt cggccgtcgc cgtcgaggag aagtcgctca tcgactacaa
     1021 ccagggcaac cccgacggcg tgctcgaccc cggtgagacc ccccgcaagc ccaaggtccc
     1081 gctggtggcg atctacccga aggaggggac gctctacagc gacagccccc tctacgtgct
     1141 cgacgccgat tgggtcagcg cccaggagaa ggagggggcc gaggcgttca tcgaggcggt
     1201 gcagcgcccg gcggcccaga agaaggtgct ggagtacggc ttccgccccg gcaaccccga
     1261 cgtcgccgtc ggcgccccca tcaccaaggg caacggcgtc gaccccaccc agccgtcgaa
     1321 gctcctccag gtcccccagc cgtcggtgat gatcgacatg ctcgagaact ggaagcagca
     1381 gcgcaagggc gcccgcgtgc tcctggtcct cgacgtctcg ggctccatgg aggagccggc
     1441 cgatcccgac gatccggcca gcgacaccaa gctcgacctg gccaagcggg ccgccatcga
     1501 cgccctcgac gagttcaagg gcgacgacga ggtcggcctc cgcgtcttca gcaccgacct
     1561 gggctcccgg gaggacgaga actacctcga cctcctcgag atccagccca tgtcgacgaa
     1621 ccgcgagcgg ctcgcatccc agatccgcga tcagctgccc cagcacgcca ccccgctcta
     1681 cgaggtgacc caggactcct tcgaggacat ggccgatggc tacgacccgt cgctgatcaa
     1741 cgccgtggtg ctgctgaccg acggcgtgaa cgacgacggc gacacctccg acgaccgcga
     1801 ccagctcgat gagctcctca ccacgctcca gtccgggacc aacggcgaga acgccacccc
     1861 catccgggtg ttcaccatcg cctacggcaa gcaggccgac ttcgacaccc tcgagcgcat
     1921 cgccgagtcg accaacgccg cggcctacga cgccagcaac cccgccacca tctcgcaggt
     1981 cttcaccgct gtggtctcca acttctgagg gcgccctcgg ccgcgaactc ggtggccgcg
     2041 cagccaccgg gtagaaacaa ggggtggcaa cggtgagctt gcgggaccgg ttcttctccc
     2101 cgcccgtcgc ccgcgccctc acctcgccct cggggatcgt cgccctcggc gccggtgcgg
     2161 ccctcggcat cttggccacg atcggcaccg gcgggctgct cggaccggtg ttcggcgggc
     2221 tgctcggcgg cgccctgggc tacgggggcc gggtggcgct cgccatcccc cgcaacgggg
     2281 cgaccgccag catcgacccg ttcggcgtca acgagccctg gcgccacgcc gtcaaagacg
     2341 ccatccaggc ccgccaccgc tacacggatg ccatcggggc gttcaaggcc gggcccctcc
     2401 gcgacgcgct gcacgagacc ggtgaccgga tcgacgacgc cgtgaacgag tgctggaagg
     2461 tcgcccagca gggccagctg ctctcgacgg cccgcaagcg catcgacgac cgggagaccc
     2521 agtgggagct gcagcaggcg gccggccaga tcccgcccgg agcgaccgcc tcgcccaccc
     2581 aggcccgcac gatcgcatcg ctcaacgccc agctcgacac cgcccgccgc atggacgcgc
     2641 tcgtcgcatc gacgtacgac gagctgcgcc tgatcaacgc ccgcctcgac gaggcggtga
     2701 cccaggccat cgagctgtcg gtcaccgacc acggtgacct cagcacggtg agcagcgacg
     2761 tcgacgacat cgtcggcagc ctcaccgccc tgcgcaccgc gatgacctcg ctcgacggga
     2821 cgggccccca ggacctcggc a
//
LOCUS       DATMFS010000658         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_22507, whole genome shotgun sequence.
ACCESSION   DATMFS010000658 DATMFS010000000
VERSION     DATMFS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22507"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2267)
                     /locus_tag="VNQ33_12600"
     CDS             complement(108..2267)
                     /locus_tag="VNQ33_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWJ98994.1"
                     /translation="MVYLSRILRFTLVMVLFVAVLTASAAVIGPQVVELVGAHRSDHE
                     RLSFKPLAERSYIYDSAGNLQGTLINAEGNRVAVELDDVPETVVGSVLAAEDQSFYEH
                     KGVNIRSIGRAVDANLQSGEVSQGGSTITQQVVKNSLVGSEQDLSRKIREAFLSVELE
                     KQMNERYCPKPIEDDCRKGKDKILERYLNSVYFGGGAYGVQAASEYYFNKNVGDLNWS
                     EGALLAALIRSPSYYDPFKNPGVAMKRRNIVFKRLLATHRLTEDEIKLYSQVPLPTTP
                     NVPLPPSDYFVEEVKQQLLDDPRFGLGATPAARNRTVFEGGIRVFTTFDPDLQFKAIQ
                     ARNETLPNNKGDGTFDVNNPKTGQVTYGTQAIASVEPSSGAVRAMVGGPGFERYQYNL
                     ATHLPGRQPGSSMKTFVLAALFENGFVPSDTVSGSSCTFDVPGQRENYSVSGKGGTNT
                     ITRQTQASNNCAFLRLGQVVGTDKVVDMAKRLGVRSNLEPVVSLPLGVFDITPIDMAA
                     AYATFANDGNYNEPYYVERIEDKNGKVLYQHQSKQVRVIDSQTARLVNATLEANVAGG
                     TGKNAQIDGGQPAAGKTGTTNGSADVWFVGYTPQLSTAIWMGAPIDRISLAQAGLGGA
                     TGGRYPATTWGRYYSMLMEDRPIVDFIAPDETRSGKSVGKVPNEIGGGSSRRGTGSRG
                     GGGGGGGTTPTTAPTPTTVPAGPGFGGGADEGQGAGE"
BASE COUNT          343 a          863 c          951 g          449 t
ORIGIN      
        1 atcgcgttcg gcacgggccg ggagcgtctc gtggtggtgg cggagctgat ccaaccgggt
       61 gtcgtgcgcc tgcacgtcga gcagggcgag cagcgccggg tggtcggtca ttcgccagct
      121 ccttggcctt cgtcggcgcc acccccgaaa ccggggcctg cgggcacggt agtgggcgtg
      181 ggcgccgtgg tcggcgtggt gcctccgcct ccgccaccgc caccgcggct cccggtgcca
      241 cggcgcgacg agccgccgcc gatctcgttg ggcaccttgc cgacggactt gccgctgcgg
      301 gtctcgtccg gcgcgatgaa gtcgacgatg ggccggtcct ccatgagcat cgagtagtag
      361 cggccccagg tggtggccgg gtagcggccg ccggtcgcgc ccccgaggcc cgcctgggcg
      421 aggctgatgc ggtcgatcgg tgcacccatc cagatggccg tggacagctg cggggtgtag
      481 ccgacgaacc acacgtcggc ggagccgttg gtggtgccgg tcttgccggc cgccggctgg
      541 ccgccgtcga tctgggcgtt cttgccggta ccaccggcga cgttggcctc gagggtcgcg
      601 ttcacgagcc gggccgtctg ggagtcgatc acccggacct gcttgctctg gtgctggtag
      661 aggaccttgc cgttcttgtc ctcgatgcgc tcgacgtagt acggctcgtt gtagttgccg
      721 tcgttggcga acgtcgcgta cgccgcggcc atgtcgatgg gcgtgatgtc gaacacgccc
      781 agcgggagcg acacgacggg ctccaggttg ctgcggacgc cgaggcgctt ggccatgtcg
      841 accaccttgt cggtgccgac gacctgcccg agccggagga aggcgcagtt gttcgacgcc
      901 tgcgtctggc gggtgatcgt gttggtgccg cccttgccgg acaccgagta gttctccctc
      961 tggccgggca cgtcgaaggt gcagctgctg ccgctgacgg tgtcgctcgg gacgaacccg
     1021 ttctcgaaca gggcggcgag cacgaacgtc ttcatcgacg agcccggctg gcggcccggg
     1081 aggtgggtgg cgaggttgta ctggtaccgc tcgaagccgg gaccgccgac catggcgcgc
     1141 accgcgccgg aggacggttc gaccgatgcg atggcctggg ttccgtaggt gacctggccg
     1201 gtcttggggt tgttgacgtc gaacgtgccg tcgcccttgt tgttgggcag cgtctcgttg
     1261 cgggcctgga tggccttgaa ctgcaggtcc gggtcgaacg tggtgaacac ccggatgccg
     1321 ccttcgaaca ccgtgcggtt gcgggccgcc ggggtggcgc cgagaccgaa gcgcgggtcg
     1381 tcgaggagct gctgcttgac ctcctcgacg aagtagtcgc tcgggggcag cgggacgttg
     1441 ggcgtggtcg gcagcggcac ctgcgagtag agcttgatct cgtcctcggt cagccggtgc
     1501 gtggccagca ggcgcttgaa cacgatgttg cggcgcttca tggcgacgcc ggggttcttg
     1561 aacgggtcgt agtagctggg gctgcggatg agcgccgcga gcagcgcgcc ctccgaccag
     1621 ttgagatcgc cgacgttctt gttgaagtag tactccgacg cggcctggac gccgtaggcg
     1681 ccgccgccga agtagacgct gttgaggtac cgctcgagga tcttgtcctt gcccttgcgg
     1741 cagtcgtctt cgatcggctt ggggcagtac cgctcgttca tctgcttctc gagctcgacc
     1801 gagaggaacg cctcgcggat cttgcggctc aggtcctgct cgctgcccac cagcgagttc
     1861 ttgaccacct gctgggtgat ggtggagccg ccctggctca cctcaccgga ctgcaggttg
     1921 gcgtcgaccg cacggccgat ggagcggatg ttgacgccct tgtgctcgta gaagctctgg
     1981 tcctcggccg ccaggaccga cccgacgacg gtctcgggca cgtcatcgag ctcgaccgcg
     2041 acccggttgc cctcggcgtt gatgagggtg ccctgcaggt tgcccgcgct gtcgtagatg
     2101 tacgagcgct cggccagggg tttgaagctc aggcgctcgt gatcggagcg gtgcgcgccc
     2161 accagctcca ccacctgggg gccgatgacg gccgccgacg cggtgagcac ggccacgaac
     2221 agcaccatga cgagcgtgaa gcggaggatg cgagacaggt agaccacggc gtgacgagcg
     2281 taccgggccg gccgtcgccg ccgatggcgc cggccgggcg gtacactcgg cgcctcgttc
     2341 accgctcggt ttccggagac ccaccccatg ctccacctcc tgcgcgcggc agctcacggc
     2401 actcccgtgc gaacggcatc gccgacggcc gcggcggact ccagggacga gcggtcccgg
     2461 tcggacggcg cgccgtgacc gacgctgacg acgcgctggc cgcgcccgcc gatgagctcc
     2521 tccgaacagg ccttccgccc gtcgccctcc tcaacccgcc gggctgatca cgtacacccg
     2581 ggccgacggc gctggggacc tcggcc
//
LOCUS       DATMFS010000659         3122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_22542, whole genome shotgun sequence.
ACCESSION   DATMFS010000659 DATMFS010000000
VERSION     DATMFS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22542"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VNQ33_12605"
     CDS             <1..223
                     /locus_tag="VNQ33_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWJ98995.1"
                     /translation="LGMPLQPILTARAIARIDRAALTPGSGAEKLANYFVGQIVGNMN
                     ETKKSADVVFEMVDEFIDAINAVRTQMDA"
     gene            302..1366
                     /locus_tag="VNQ33_12610"
     CDS             302..1366
                     /locus_tag="VNQ33_12610"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWJ98996.1"
                     /translation="MDPAPTQPTTDDAPASPDDGSTPTAAAGADAQAGAIEAGTEVPK
                     KGIAKLRHRADVVTGQGKKLVDDTRATVPPVDIGFSAVERDNHIGGFLLAGAIAFRLF
                     VFVLPMYLLALVIAGAIFAYDPDSTKDVADSAGMSKYMASSIGDAAQTSHKSLWLLIP
                     VTLYAMISAGRSVEKAIAAAHARAWSIPLPKRKPHYVVLGVFGFALSVLTATRIVSVI
                     RHGALVPVSMVLGAAIYFTLWLLASMALPRREGVTWWSLIPGAVLMGTGTQALYLFNV
                     LYLNHKIQSASAAYGALGVAASALLWLYLLGRLMVASPVLNATIWQRQHDPDGALSPN
                     AGSDLPGLSLGDEPKPSPAD"
     gene            complement(1375..1710)
                     /locus_tag="VNQ33_12615"
     CDS             complement(1375..1710)
                     /locus_tag="VNQ33_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ98997.1"
                     /translation="MATFSAEHAVPVDVVGARQAADPVTGAEAIAFALQPDDGGDLQV
                     FSLSICTAYDLREVIDEALEAPFSWDDGESGPTGLDELDGLAGSNVIELPRPPSARWG
                     EAWATSHPR"
     gene            1773..2882
                     /locus_tag="VNQ33_12620"
     CDS             1773..2882
                     /locus_tag="VNQ33_12620"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWJ98998.1"
                     /translation="MRPIRRSGMLGGIAARTADRRLRAARLGSQLLTSRDAASPEAVV
                     DQLLAVQAQDQRAARLSVRSRSTGLLATDVDEALTDRRTLVIGSLMRTTLHLVAADDY
                     WWLHDLLTPRQRTANTTRLRQEGVNEDQAERGVAVIAEAVQSGPVVRDQIRALLDAAK
                     VPTAGQALVHVQHAAAIGGHTVRGPMVDGHHAFVHAPTWLGERPDEDRGVQLARLVER
                     YLRGHGPAAPEDLVKWTGLSLGECRTAFATIDDRTEPWGTDGLVRLSGQPVETDELPA
                     PRLLGGFDPILHGWVSRKEFVEEDDGVVTSNGIFRPTALVDGRAAATWTMPENQITLK
                     PITKIPEKAVTALEDEAEDVARYLGIAPRPLAIED"
BASE COUNT          467 a         1157 c         1058 g          440 t
ORIGIN      
        1 gctcggcatg ccgctccagc ccatcctcac ggcccgcgcc atcgcccgca tcgaccgggc
       61 cgccctgacg cccgggtccg gcgccgagaa gctggccaac tacttcgtgg gccagatcgt
      121 cgggaacatg aacgagacga agaagtcggc cgacgtggtc ttcgagatgg tcgacgagtt
      181 catcgacgcc atcaacgccg tccgcaccca gatggacgcc tgagccaccg ctcgcctcac
      241 gtccgggccg acccggccga tacgttgcgg acgccccttc tgcccgcaac caggaactcc
      301 cgtggacccg gccccgacgc aaccgacgac cgacgacgcc ccggcgtcgc ctgacgacgg
      361 ctcgacgccg acggccgccg ccggagcgga cgcccaggcc ggggcgatcg aggccggcac
      421 cgaggtgccg aagaagggca tcgccaagct gcgccaccga gccgacgtgg tgaccggcca
      481 gggcaagaag ctcgtcgacg acacccgcgc gaccgtgccg cccgtggaca tcgggttctc
      541 cgccgtggaa cgggacaacc acatcggcgg cttcttgctg gcgggcgcga tcgccttccg
      601 gttgttcgtg ttcgtgctgc cgatgtacct gttggcgctg gtgatcgccg gggcgatctt
      661 cgcctacgac ccggactcga cgaaggacgt ggccgacagc gccggcatgt cgaagtacat
      721 ggcgagctcc atcggcgatg cggcgcagac atcgcacaag agcctctggc tgctcatccc
      781 ggtgaccctc tacgccatga tctccgccgg gcggtcggtg gagaaggcca tcgccgcggc
      841 ccacgcccgg gcgtggagca tcccgctccc caagcgcaag ccccactacg tggtgctcgg
      901 cgtgttcggg ttcgcactgt cggtcctcac cgccacgcgc atcgtgtcgg tgatccgcca
      961 cggcgccctc gtgccggtgt cgatggtgct gggtgccgcc atctacttca cgctctggct
     1021 cttggcctcg atggcgcttc cccgccgcga gggcgtgacc tggtggtcgc tgatccccgg
     1081 ggcggtcctc atggggacgg gcacccaggc gctgtacctg ttcaacgtct tgtacctgaa
     1141 ccacaagatc caatcggctt ccgctgcgta cggcgcgctg ggcgtggcgg cctcggccct
     1201 gctgtggctg tacctgctcg gccggctcat ggtcgcgtcg cccgtgctca acgccacgat
     1261 ctggcagcgc cagcacgacc cggacggagc gctctccccc aacgccggca gcgacctccc
     1321 cgggctcagc ctcggtgacg agccgaagcc atccccggcg gactgaccca gcgctcatcg
     1381 cgggtgcgag gtggcccagg cctcgcccca ccgcgccgac ggcgggcggg gcagctcgat
     1441 cacgttcgag ccagccaggc cgtcgagctc atcgagcccc gtcgggccgc tctcgccgtc
     1501 gtcccaggag aacggcgcct cgagcgcctc gtcgatgacc tcacgcaggt cgtaggcggt
     1561 gcagatgctg agcgagaaga cctgcaggtc gccgccgtcg tcgggttgga gggcgaaggc
     1621 gatggcctcc gcgcccgtca ccggatcggc agcctgacgg gcgccgacga cgtcgacggg
     1681 caccgcgtgt tccgcagaga aggtggccat ggatctgctc cttgttcggg ggagcagcac
     1741 cgtacgaagg gggtctgaca acgaagccgt gcgtgcgacc catccgtcgc tcgggcatgc
     1801 tcggaggcat cgctgctcgc accgctgacc gccggctccg tgctgctcga ctcgggtcgc
     1861 agctgctcac ctcacgtgat gcggcatcgc ctgaggcggt ggtcgaccag ctgctcgccg
     1921 tccaggcgca ggaccagcgg gccgcccggc tgagcgtccg ctctcgctcc acgggcctgc
     1981 tcgccaccga cgtcgacgag gcgctcaccg atcgcaggac gctcgtgatc gggtcgctca
     2041 tgcggaccac gctccacctc gtggccgctg acgactactg gtggctgcac gacctcctca
     2101 ccccgaggca gcgcacggcc aacaccaccc gcctccgcca ggagggcgtg aacgaggatc
     2161 aggccgaacg cggcgtggcc gtcatcgccg aggcggtgca gagcggcccc gtggtccgcg
     2221 accagatccg tgcgctgctc gacgcggcga aggtgccgac cgccggccag gccctcgtcc
     2281 acgtgcagca cgcggcggcg atcgggggcc ataccgtccg gggtccgatg gtggacggcc
     2341 accacgcctt cgtccacgcc cccacctggc tcggcgagcg gcccgacgag gaccgcggcg
     2401 tccagctcgc ccgcctcgtc gagcgctacc tgcgcggcca cgggcccgct gctcccgagg
     2461 acctcgtgaa gtggaccggc ctctccctcg gcgagtgccg cacggccttc gccaccatcg
     2521 acgaccgcac cgaaccctgg ggcaccgacg gcctcgtccg cctgagcggt caacccgtcg
     2581 agaccgacga gctcccggcc ccgaggctgc tcggcggctt cgaccccatc ctccacggct
     2641 gggtgagccg caaggagttc gtggaggaag acgacggcgt cgtgacgagc aacggcatct
     2701 tccgcccgac ggcgctggtc gacggccgag cagcggccac gtggaccatg cccgagaacc
     2761 agatcaccct gaagccgatc accaagatcc ccgagaaggc ggtgaccgct ctggaggatg
     2821 aggccgaaga cgttgcccgc tacctcggca tcgccccgcg gccgttggcg atcgaggact
     2881 gagcagcgcc agatctcggt tcgcaccccg tcggcgagga cgacgagcat gtcgccctcg
     2941 ttcccgagct cgaggagcag gcgtcgagcg agtgccgtcc gttcgcaccg ggcacgcggc
     3001 tcgaccgatc cggaagaccc cgatgaggct cagggcttgg cgatcgggag ctcgatcggg
     3061 ccgcgcaggg tcatggagcg cttccacgcc acgccggtgt gctggacgcg gtggtcgggg
     3121 aa
//
LOCUS       DATMFS010000660         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_183016, whole genome shotgun sequence.
ACCESSION   DATMFS010000660 DATMFS010000000
VERSION     DATMFS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183016"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="tatC"
                     /locus_tag="VNQ33_12625"
     CDS             <1..665
                     /gene="tatC"
                     /locus_tag="VNQ33_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWJ98999.1"
                     /translation="LGWVLYNPVLSWLREPLEAISDDPNVSDNLRFLGPMDGFMFRIK
                     VSAYLGLAICMPYLLWQLWRFISPGLYQNERRYASAFVGSATALFLMGAYVAYFTLPA
                     ALSFLTSVGGNDVTYEFTPEKYVMLIVYMMLAFGVGFEFPIVLVALQLVGLIKPQQLR
                     DFRRFGIVIIFVIAAVITPSADPISLFALAIPMMVFYEISIVIGRIIVGRRAAGEVSS
                     GP"
     gene            662..>2381
                     /locus_tag="VNQ33_12630"
     CDS             662..>2381
                     /locus_tag="VNQ33_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ99000.1"
                     /translation="MTEEPFVLDRFQREALAAIDLDRNVLVAAPTGAGKTVVAEHAVA
                     RALAAGRRAFYTTPIKALSNQKYHDLVRRYGRDRVGLLTGDNAVNPGAGVVVMTTEVL
                     RNMVYAASPALDGLAWVVLDEVHFLQDSYRGPVWEEVIVHTPPSVRFVCLSATVSNAD
                     ELAGWISAARGPTETVVEHERPVDLVNHFLVYDKGTGDLVQVETLVDGRPNPEGTRFD
                     APALDGPKHERFKARGRHRWGTPRRVEVVEHLAGSGLLPAISFVFSRKGCDDAARSVL
                     DAGLRLTTGPERERIRAIVDAHVDHLSDGDLSVLGFPRFLAGLEAGVAAHHAGMVPPF
                     KEAVEACFVEGLVKVVFATETLALGINMPARSVVIESLSKFTGEHHEDLTPSQYTQLT
                     GRAGRRGLDPVGHALVLWSPWYGFDKVAALAASREFVLRSAFQPTCNMVVNLVRRYDR
                     EQAHELLGRSFAQFQADASLGRIVNRRASRQQLLADATARATCERGDVDEYRALRADE
                     RRRAREARQGARARIADQAAALAPGDVVQVAGHRLAVLSVSHRKGGLRLRVVDGDARV
                     SVLDEDD"
BASE COUNT          318 a          852 c          831 g          380 t
ORIGIN      
        1 cgctcggctg ggtgctctac aacccggtcc tgagctggct gcgcgagccg ctcgaggcca
       61 tctccgacga cccgaacgtc agcgacaacc tgcgcttcct cgggccgatg gacgggttca
      121 tgttccgcat caaggtgtcc gcctacctcg gcctcgccat ctgcatgccc tacctcttgt
      181 ggcagctctg gcggttcatc tcaccggggc tgtaccagaa cgagcgccgc tacgcctcgg
      241 ccttcgtggg atcggccacc gccctcttcc tcatgggcgc ctacgtcgcg tacttcaccc
      301 tgcccgccgc gctcagcttc ttgacctcgg tcggcggcaa cgacgtgacc tacgagttca
      361 cgcccgagaa gtacgtgatg ctgatcgtgt acatgatgct ggcgttcggc gtcggcttcg
      421 agttcccgat cgtgctcgtc gcgctccagc tggtgggcct catcaagccg cagcagcttc
      481 gcgacttccg tcgcttcggc atcgtcatca tcttcgtgat cgccgccgtg atcacgccga
      541 gcgccgatcc gatcagcctc ttcgccctcg ccatcccgat gatggtcttc tacgagatct
      601 ccatcgtcat cggccggatc atcgtcgggc ggagggcggc cggcgaggtc agctccggac
      661 cgtgaccgaa gagccgttcg tcctcgaccg cttccagcgg gaggcgctcg cggccatcga
      721 cctcgaccgc aacgtgctgg tggctgcgcc caccggcgcc ggcaagacgg tggtggccga
      781 gcatgccgtg gcacgcgccc tcgccgccgg ccgccgggcg ttctacacga cgcccatcaa
      841 ggcgctgtcg aaccagaagt accacgacct ggtgcgccgc tacggccgcg accgggtggg
      901 cctgctcacc ggtgacaacg ccgtgaaccc gggcgccggg gtggtggtga tgaccaccga
      961 ggtgctgcgg aacatggtct acgccgcgtc gcccgcgctc gacggcctcg cctgggtcgt
     1021 gctcgacgag gtccacttcc tgcaggactc ctaccgggga ccggtgtggg aggaggtcat
     1081 cgtccacacg ccgccgtcgg tccggttcgt gtgcctgtcg gccaccgtct ccaacgccga
     1141 cgagctggcc ggctggatca gcgcagcacg gggaccgacc gagacggtgg tcgagcacga
     1201 gcggccggtc gacctggtca accacttcct cgtctacgac aagggcaccg gtgacctcgt
     1261 ccaggtcgag acgctggtcg acggccggcc caaccccgag ggcacccggt tcgacgcgcc
     1321 ggctctcgac gggcccaagc acgagcggtt caaggcgcgc ggccgccacc ggtggggcac
     1381 gcctcgccgg gtcgaggtgg tcgagcacct ggcggggagc gggctgctgc ccgcgatctc
     1441 cttcgtgttc agccgcaagg ggtgcgacga cgcggcccgt tccgtgctcg acgccgggtt
     1501 gcggctcacc accggtcccg agcgggagcg gatccgggcc atcgtcgacg cccacgtcga
     1561 ccacctgagc gacggcgact tgtcggtgct gggcttcccg cggttcctcg ccggcttgga
     1621 ggccggggtc gccgcccacc acgccgggat ggtgccgccg ttcaaagagg cggtggaggc
     1681 ctgcttcgtg gaggggctgg tcaaggtggt gttcgccacc gagacgctgg ccctcggcat
     1741 caacatgccg gcgcggtcgg tggtgatcga gtcgctctcg aagttcaccg gcgagcacca
     1801 cgaggacctg acaccgtcgc agtacacgca gctcaccggg cgggccggcc gccgcggctt
     1861 ggatcccgtc ggccatgccc tggtcctgtg gtcgccctgg tacgggttcg acaaggtggc
     1921 ggcgctggcg gcgagccggg agttcgtgtt gcggtcggcc ttccagccca cctgcaacat
     1981 ggtcgtcaac ctggtccgcc ggtacgaccg cgagcaggcc cacgagctgc tcggacggtc
     2041 gttcgcccag ttccaggccg atgcctcgct cggacggatc gtgaaccgcc gagcgagccg
     2101 gcagcagctg ctggccgatg cgacggcgcg ggcgacctgc gagcggggcg acgtcgacga
     2161 gtaccgggcg ctgcgggccg acgagcggcg ccgcgcgcgc gaggcgcgcc aaggggcccg
     2221 ggcccggatc gccgaccagg ccgcggcgct cgcccccggc gacgtggtgc aggtggccgg
     2281 ccaccgcctg gcggtgctgt ccgtgtccca ccgcaagggc ggcctgcgcc tgcgggtggt
     2341 cgacggcgat gcccgggtgt ccgtgctcga cgaggacgac t
//
LOCUS       DATMFS010000661         3130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_92549, whole genome shotgun sequence.
ACCESSION   DATMFS010000661 DATMFS010000000
VERSION     DATMFS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92549"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VNQ33_12635"
     CDS             <1..850
                     /locus_tag="VNQ33_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWJ99001.1"
                     /translation="LTSIATGLAPGEHGVMGYRVAVDHEVLNILRWTTPVGDARKRIH
                     PQDFQPNPAFCSQRPCIVTKAEFATSGFSGAHLAEVRFRGYRVPSTMVVELRQALKAG
                     EPFVYVYYDGIDKVAHEYGLAQHYDAELQAVDRLVADILSVLPRGAAVVVTADHGQVD
                     VGDRVIDPSADVLAHVSFQSGEGRFRWLHARPGREGALLEAAQAHHSDTAWVVSRDQT
                     VDEGWWGERVTDAARQRLGDVALVAHEPVSFNDAADSGPFQLIGRHGSLTPAEMYVPL
                     LVGEAR"
     gene            847..1392
                     /locus_tag="VNQ33_12640"
     CDS             847..1392
                     /locus_tag="VNQ33_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HWJ99002.1"
                     /translation="MTDPQPDPTPEVAHGELVAHGPEAVEPAEADERREAVEEPAKVM
                     RIGSMIKQLLDEVRQADLDGPARERLKAIYDSSIQEVGQALSPDLRAELERVAIPFGD
                     DSEPSDAELRIAQAQLVGWLEGLFHGIQATLFAQQMAARNQLEQMRGQLPPGAMPGGS
                     GPGGPGGPTADVGDVRPGTYL"
     gene            complement(1389..2486)
                     /locus_tag="VNQ33_12645"
     CDS             complement(1389..2486)
                     /locus_tag="VNQ33_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99003.1"
                     /translation="MNRLDDAFEALIAQHPEGDPDMVIATARQAAATSRARHRTVLAL
                     ASAAAVVVVAAASAFAVAGTGEPASQIHAGPGLTATTAVTTPDTPATKGSGHAIASPA
                     GVALRSGTDPATDLPITGEPAVAAYAVGTELVVYQGADPDPGDQAWPWPAGDIAVWSR
                     HGTKTVAIPDGTDRAELLDAGTVDGSPKALVALYHGDGFDTSSVTLALIDLHTLSSHT
                     VVDHPAWESGYTQARVLPDGDVIGLLTVEASSSLVRWTPGRPDAVWSTPAPDLDRTLA
                     LRGDRAVLIDTQQPGKVTTYPVDLATGALGTPATTPTTGVDGDLSCRDWYDDQALSCA
                     RPTAPAGTIGSQGTWHQLPTPDGATVTASRD"
     gene            complement(2483..2950)
                     /locus_tag="VNQ33_12650"
     CDS             complement(2483..2950)
                     /locus_tag="VNQ33_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ99004.1"
                     /translation="MEDELFRSIYPALHRLAAVVCPAERDAEDLVQEALVRTLRRGPL
                     SELDSPLAYLRRTVVNLAANERRSLGRLRRAAPRLAAVADEGEAPSYPSDVAVLLQLG
                     PLDRAAVWLSAVEGQSAEHIGQVLGCSPEAARTRVSRAHKRLRTLLDEEGTTR"
BASE COUNT          428 a         1115 c         1161 g          426 t
ORIGIN      
        1 gctcacgagc atcgccacgg ggctcgctcc cggcgagcac ggcgtcatgg ggtaccgggt
       61 cgccgtggat cacgaggtcc tgaacatcct ccggtggacc acgccggtgg gcgatgcccg
      121 caagcgcatc cacccgcagg acttccagcc caacccggcg ttctgcagcc agcggccgtg
      181 catcgtcacc aaggcggagt tcgccacgtc gggcttcagc ggcgcccacc tcgccgaggt
      241 ccgcttccgc ggctaccggg tcccgtcgac gatggtcgtg gagctgcgcc aggccctcaa
      301 ggcgggggag ccgttcgtgt acgtgtacta cgacggcatc gacaaggtcg cccacgagta
      361 cgggctcgcc cagcactacg acgcggagct ccaggcggtg gatcggctcg tggccgacat
      421 cctctcggtg ctgccgcgcg gcgccgccgt ggtcgtcacc gccgaccacg gccaggtcga
      481 tgtgggcgac cgcgtcatcg accccagcgc cgacgtcttg gcccacgtgt cgttccagtc
      541 cggcgagggc cgcttccggt ggctgcacgc ccggccgggc cgcgagggcg ccctgctgga
      601 ggcggcgcag gcacaccact ccgacaccgc ctgggtcgtg tctcgggacc agaccgtgga
      661 cgaagggtgg tggggtgagc gcgtcaccga cgcggcccgc cagcgcctgg gcgacgtcgc
      721 cctcgtcgcc cacgagccgg tctcgttcaa cgacgcggcc gactccggcc cgttccagct
      781 catcggccgc cacgggtccc tcaccccggc cgagatgtac gtccccctgc tcgtaggaga
      841 agccagatga ccgaccccca gcctgacccc acgcccgagg tggcccacgg cgagctcgtc
      901 gcccacggcc ccgaggcagt ggagccggcg gaggccgacg agcgtcggga ggccgtggag
      961 gagccggcca aggtcatgcg catcggctcg atgatcaagc agctgctcga cgaggtccgc
     1021 caggccgatc tcgacggtcc cgctcgtgag cggttgaagg ccatctacga cagctcgatc
     1081 caagaggtcg gtcaggcgct gtcgccggat ctgcgggccg agctggagcg ggtggccatc
     1141 ccgttcggcg atgacagcga gccgagcgat gccgagctgc gcatcgccca ggcccagctc
     1201 gtcgggtggc tcgagggcct cttccacggc atccaggcca cgctgttcgc ccagcagatg
     1261 gcggcccgca accagctcga gcagatgcgc ggccagctcc cgcccggcgc catgccgggc
     1321 gggagcggcc ctggcggccc cgggggaccg accgccgatg tcggcgacgt gcgccccggc
     1381 acctacctct agtctcggct ggcggtgacg gttgctccgt cgggggtggg gagctggtgc
     1441 caggttccct gggagccgat ggtgcctgct ggggcggtgg ggcgggcgca gctgagggcc
     1501 tggtcgtcgt accagtcgcg gcacgacagg tcgccgtcga cgccggtggt gggggtggtg
     1561 gcgggggtgc cgagggcgcc ggtggccagg tcgaccgggt aggtggtgac cttgcctggc
     1621 tgctgggtgt cgatcagcac ggcgcggtcg ccgcgcaggg ccagggtgcg gtccaggtcg
     1681 ggtgccgggg tggaccagac tgcgtcgggt cggccggggg tccagcggac gagcgacgag
     1741 cttgcctcca cggtgagcag cccgatgacg tcgccgtcgg gcagcacccg ggcctgcgtg
     1801 tagccggact cccatgccgg gtggtcgacg acggtgtggg agctgagggt gtgcaggtcg
     1861 atcagcgcga gggtcacgct gctggtgtcg aagccgtcgc cgtggtacag ggcgacgagg
     1921 gccttgggtg agccgtcgac ggttccggcg tcgagcagct cggcgcggtc ggtcccgtcg
     1981 gggatcgcca cggtcttggt gccgtggcgg gaccacaccg cgatgtcgcc ggcaggccac
     2041 ggccaggcct gatcgccggg gtcggggtcg gcgccttggt agacgaccag ctcggtgccg
     2101 acggcgtacg ccgccaccgc cggctccccg gtgatcggca ggtcggtggc cgggtcggtg
     2161 cccgagcgca gcgcgacgcc tgccggtgag gcgatggcgt ggcccgaacc cttcgtcgcc
     2221 ggtgtgtcgg gggtggtcac ggcggtggtg gcggtcaggc cggggccggc gtggatctgg
     2281 cttgcgggtt cgccggtccc ggcgaccgcg aacgccgacg cggcggccac gacgacgacg
     2341 gcggcagccg aagccagcgc gaggaccgtg cggtgccgcg cccggctcgt tgcggctgcc
     2401 tggcgggcag tggcgatcac catgtcggga tcgccctcag ggtgctgggc gatcagggcc
     2461 tcgaaagcat cgtcaagccg gttcatcgtg tcgttccttc ctcatcgaga agcgtgcgca
     2521 ggcgcttgtg ggccctcgac acgcgggtac gggcggcctc gggcgaacag ccgaggacct
     2581 ggccgatgtg ctcggccgac tggccttcga cggcgctgag ccacacggcg gcccggtcga
     2641 gcggaccgag ctgcagcagc accgccacat cagacggata ggagggcgcc tcgccctcgt
     2701 cagccaccgc ggcgagccgc ggcgccgccc gccgcaggcg gcccaggctc cggcgctcgt
     2761 tcgccgccag gttcaccacc gtgcgccgca ggtacgccaa cggcgagtcc agctcgctca
     2821 gcggcccacg gcgcaaggtg cgcaccagcg cctcttgcac cagatcctcc gcatcacgct
     2881 cagccggaca caccaccgcc gcgagacggt gcagcgccgg atagatcgaa cggaacagct
     2941 cgtcttccac caacgacccg gccgttcgga ccgatctgac tgaacggatc ggccgaaccg
     3001 gccggtcggg tggacgtttc atcaccacat cacctgtgct cataccccca agacaccgca
     3061 ccgccacggc acgtgacacc acggcacaac ccgcccacca cctcgacccc gcgccggacc
     3121 ggctccccgc
//
LOCUS       DATMFS010000662         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_253480, whole genome shotgun sequence.
ACCESSION   DATMFS010000662 DATMFS010000000
VERSION     DATMFS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253480"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNQ33_12655"
     CDS             <1..398
                     /locus_tag="VNQ33_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HWJ99005.1"
                     /translation="RGTPRIANRLLRRVRDFAEVRGTGVVDAAAAQAGLKVFGVDERG
                     LDKVDRAILSAICERFGGGPVGLSTLAISVAEPTETVEDVYEPFLIREGLLMRTPRGR
                     VAMPAAWQHLGLAAPAVAPDHAPPPGLFG"
     gene            862..1323
                     /locus_tag="VNQ33_12660"
     CDS             862..1323
                     /locus_tag="VNQ33_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWJ99006.1"
                     /translation="MASSAVPAKPEWGRIMRRGAFKRCPRCGSGHLFEGWFRLKEHCP
                     RCGMRFEREEGAFTGVYLLNFGVDLFLLWALMMVYIGMLATADGEPVNAVPIGIAAAV
                     VAIVFPVVFYPYAKTIWIAIHLGAEALSPAELEEAEAHRSVPTEVLALASR"
     gene            1395..2534
                     /locus_tag="VNQ33_12665"
     CDS             1395..2534
                     /locus_tag="VNQ33_12665"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWJ99007.1"
                     /translation="MQIPFNASVGPSLGIEVELELVDVTTGQLASHASEILTELGAGH
                     PDGEHPRAKHELFECCVEVITDVCSTVGEARTDLAATIAEVREAAGRRGCTLICSGSH
                     PYSLWGEQRVSPCERYDELVEAMQWMARRMQIFGIHLHLGVRSGEKAIALGNAMATYI
                     PHLLALSSSSPYWEGHDTGLASTRSKIFEGLPTAGLPVALADWDEFEQYMTTLVAAGA
                     ITSVREVWWDIRPHPDFGTIELRMCDGMPTLFEVSAVAALAQSLLAHLDRLHDAGELP
                     ERPREWILRENKWRAGRFGLGADLIVDRGGATKTACEAIAELVEQVRPEAEVLRCSEE
                     LAGIGRMLEVGSSASRQRRASDGGKNLRAVVDLLARELATDEIGA"
     gene            2531..3733
                     /locus_tag="VNQ33_12670"
     CDS             2531..3733
                     /locus_tag="VNQ33_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWJ99008.1"
                     /translation="MSTPAVTRVAAVIDALAPELVAFRRDLHAHPEPSWEEHRTTGLL
                     LDRLRLAGLDPHVAPTPAGTGVICDLGTEGPMVALRGDLDALRLHDTKEVPYRSTVDG
                     VCHACGHDLHATAVLGAALALAEALPERNGSGRVRIILQPAEESVPGGASTLLAAGVV
                     DDVEAVFALHADPSRAVGSLGISAGPVTSAADQLDIRLYGPGGHTGRPHESVDLAHLA
                     ARVVLELPMSLSRLSDPRDGLNLTFGSIQVGDAPNVIATEARLMASLRASGRGAWESA
                     PTHLERLVASIVEPLGATWELDHRRGAPPVVNDPWAAAIAAAAAGEVVGEERVGTTEQ
                     SAGGEDFSWYGEKARLGYLRLGVWDPARLRTDLHAGGFDLDESAIAIGAKVLAGAALR
                     ALDDLTDH"
BASE COUNT          492 a         1394 c         1391 g          567 t
ORIGIN      
        1 cgcggggcac cccgcgcatc gccaaccgcc tcctgcgccg ggtccgtgac ttcgccgagg
       61 tccgaggcac cggtgtggtc gatgccgcgg ccgcccaggc cgggctcaag gtgttcggtg
      121 tcgacgagcg gggactcgac aaggtcgacc gggccatctt gtcggccatc tgcgagcgct
      181 tcggcggcgg tccggtcggg ctctccaccc tcgccatcag cgtggccgag cccaccgaga
      241 cggtcgaaga cgtgtacgag cccttcctca tccgcgaggg cctgctcatg cgcacgccga
      301 gaggccgcgt cgccatgccc gccgcctggc agcacctcgg cctcgccgcc ccggcggtcg
      361 cccccgacca cgccccgccc ccgggtctgt tcggctgagc gctcggaggc gccgtcgacc
      421 gtggcggtga cacggcgcag gggaggtacg gtgccggcga cttgggaccg tgagcctcgc
      481 agcgtggaga tggtcggatt cggggtgttc ttcgtcgtcc tccttggttg ggatgggggc
      541 accggtccgg agggttcgtg gagatggtcg gattcggggt gttcttcgtc gtctggctcg
      601 tcctcgtcgc cgtctcactc ggagggttgg tctgtgccgt ggcggcgctc atcagcatct
      661 cccggacgaa cgcagatgcg ttcggcccgt ggtgggacaa cacgaaggcg ccgtggctcc
      721 ttggcgtacg gaggaggtct gacagtggag cggtgttccg gcccctttcc ggtcatgtcc
      781 gattccgggg gcagccggct ggcgcggcgg gcggcatcgc tgcgttggcc cggccgggct
      841 tctttggact aggccagcct cgtggcttcg agcgcagtcc cggcgaaacc ggagtggggg
      901 cggatcatgc ggcgcggcgc gttcaagcgc tgcccccgct gcgggagcgg gcacctcttc
      961 gagggctggt tccgcctgaa ggagcactgc ccgcgctgcg ggatgcgctt cgagcgggag
     1021 gagggcgcgt tcaccggcgt ctacctcctg aacttcggtg tcgacctctt cctgctgtgg
     1081 gcgctgatga tggtgtacat cggcatgctc gccaccgccg acggcgagcc ggtgaacgcc
     1141 gtgccgatcg gcatcgccgc cgcggtcgtc gccatcgtct tccccgtggt cttctacccg
     1201 tacgccaaga cgatctggat cgccatccac ctcggcgccg aggccctcag ccccgcggag
     1261 ctggaagaag ccgaagcgca ccgctccgtc ccgacggaag tcctggctct cgccagtcgc
     1321 tgacgggcgc gccgtcgggg ccgatcccag cccggtcaga caggcccggc gccgccaccg
     1381 gtagcctgcg ccgggtgcag atcccgttca acgcgtcggt ggggccgagc ctggggatcg
     1441 aggtggagct cgagctggtc gacgtgacga cggggcagct ggcgtcgcac gcatccgaga
     1501 tcctcaccga gctcggtgcc ggtcatcccg acggcgagca cccccgtgcc aaacacgagc
     1561 tgttcgagtg ctgcgtcgag gtcatcaccg atgtctgctc cacggtgggc gaggcccgga
     1621 ccgacctggc ggccaccatc gccgaggtcc gcgaggccgc cggccgccgg ggttgcacgc
     1681 tcatctgctc gggcagccac ccctactcgc tgtggggcga gcagcgggtc agcccctgcg
     1741 agcgctacga cgagctggtc gaggccatgc agtggatggc tcggcggatg cagatcttcg
     1801 ggatccacct ccacctcggc gtgcgctcgg gggagaaggc catcgccctc ggcaacgcca
     1861 tggccaccta catcccgcac ctcctcgccc tgtcctcatc gagtccgtac tgggagggac
     1921 acgacaccgg gctggcctcg acccgttcga agatcttcga aggcctgccc acggcgggcc
     1981 ttccggtcgc gctggccgac tgggacgagt tcgagcagta catgaccacg ctcgtcgcgg
     2041 ctggggccat cacgtcggtc cgggaggtgt ggtgggacat ccggccccac cccgacttcg
     2101 gcacgatcga gctgcggatg tgcgacggca tgcccacgct cttcgaggtg tcggccgtcg
     2161 ccgcgctggc gcagagcctg ctggcgcacc tcgaccggct ccacgacgcc ggtgagctcc
     2221 ccgagcggcc acgggagtgg atcctgcggg agaacaagtg gcgagccggc cgcttcgggc
     2281 tgggggccga tctcatcgtc gatcggggcg gggccaccaa gacggcgtgc gaggccatcg
     2341 ccgagctggt cgagcaggtg cggcccgagg ccgaggtcct gcgctgcagc gaggagctgg
     2401 ccggcatcgg ccgcatgctg gaggtcggtt cgagcgcgtc tcggcaacgg cgggcgagcg
     2461 acggcgggaa gaacctccgt gccgtcgtgg acctcctggc ccgtgagctg gcgaccgacg
     2521 agatcggcgc atgagcacgc ccgccgtcac ccgcgtcgcc gccgtcatcg acgccctggc
     2581 gcccgagctg gtggccttcc ggcgcgacct ccacgcccac ccggagccgt cgtgggagga
     2641 gcaccggacc acgggcctcc tcctcgaccg cctccgcctg gccgggttgg atccccacgt
     2701 ggcgccgacg cccgcgggca ccggcgtcat ctgcgacctc gggaccgagg ggccgatggt
     2761 cgccctgcgc ggcgacctcg acgccctgcg cctccacgac acgaaggagg tgccgtaccg
     2821 ctcgacggtg gatggcgtgt gccacgcatg cggccacgat ctgcacgcca ccgcggtgct
     2881 cggtgcggcc ctggcgctgg ccgaggccct gcccgagcgc aacggatcgg gacgggtgcg
     2941 gatcatcctc caaccggccg aggagtcggt gcccggcggc gcgtcgacgc tgctcgcggc
     3001 cggcgtggtg gacgacgtcg aggcggtctt cgccctccac gccgatccct cacgggcggt
     3061 cgggtcgctc gggatcagcg ccgggccggt gacctcggcg gccgatcagc tggacatccg
     3121 gctctacgga cccggtggcc acaccggccg cccgcacgag tcggtggacc tcgcccacct
     3181 ggccgcccgg gtggtgctcg agctgccgat gagcctcagc cgcctgtcgg accctcgcga
     3241 cggcctcaac ctcacgttcg gttcgatcca ggtaggcgat gcgcccaacg tgatcgcgac
     3301 ggaggcgcgc ctgatggcct cgctgcgcgc ctcggggcgc ggggcctggg agtcggcccc
     3361 gacgcacctc gaacggctcg tcgcctcgat cgtggagccg ctcggcgcca cctgggagct
     3421 cgaccaccgg cggggcgccc cgcccgtcgt caacgacccg tgggccgcgg cgatcgcggc
     3481 cgcggcagcg ggcgaggtcg tgggggagga gcgggtgggc accaccgagc agagcgccgg
     3541 aggcgaggac ttctcctggt acggcgagaa ggcccggctc ggctacctcc gcctcggcgt
     3601 ctgggacccg gcgcggctgc gcaccgacct gcacgccggc ggcttcgacc tcgacgagtc
     3661 ggccatcgcc atcggcgcca aggtcctcgc cggcgccgcc ctgcgagcac tcgacgacct
     3721 gaccgaccac tgaccgcggc acgagcctgg cggctcggag acgctgctcc tacgatcgcc
     3781 cgtcccatgg acaccgccga cctcgactac gggctgcccg cgtcgtccat cgcccagacg
     3841 ccgg
//
LOCUS       DATMFS010000663         4508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_22852, whole genome shotgun sequence.
ACCESSION   DATMFS010000663 DATMFS010000000
VERSION     DATMFS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22852"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VNQ33_12675"
     CDS             <1..440
                     /locus_tag="VNQ33_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ99009.1"
                     /translation="IGGSGEKKTLRLVAQYADESNLTAPIEEVPRKLDALAAHCERLG
                     RDRSEITVSVMGRACVAPTHDQAKAELDAALLSRGLDLSSMSEEDAEAMRSLIPYGDP
                     DEIAEHFSGYLIDGVDGLTVSAIANGHLEGRVSLLGETLSKLF"
     gene            complement(437..1159)
                     /locus_tag="VNQ33_12680"
     CDS             complement(437..1159)
                     /locus_tag="VNQ33_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWJ99010.1"
                     /translation="MPELEAVPEDWSRALAVVAHPDDLEYGAASAIARWTDQGKAVAY
                     CLVTSGEAGIDSMAPDACARVRQNEERASAAAVGVDVVEFLAHPDGLVEANTALRRDL
                     AAAIRRHRPEVVISINFRETFGFPGLNHADHRNTGIALLDAVRDAANRWLFVGAGGGP
                     WNGVRFALFGGSPQATHGVDITDTLDRGIASLEAHRVYLDALAEGTTGTNPDEFLRGM
                     ATQAGPRLGVDLATTFELIPLG"
     gene            1193..3025
                     /locus_tag="VNQ33_12685"
     CDS             1193..3025
                     /locus_tag="VNQ33_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ99011.1"
                     /translation="MPAPEPAVHRLVAPMAATVASVAVADGDHVRDGQTVIVLEVMKM
                     EHPLQVFLDGTISGLAVAEGDAVSQGQELLKITAAPAPGAAREPAAVEETAPTGEERA
                     DLRELRRRRALLEDAARPGAVAQRHGRGRRTARENVADLCDDGSFEEHGGFAFAAQEG
                     RRDRADLQANTPADGLITGIGRVDGAPTAVVAYDYTVLAGTQGQRNHAKKDRIFELIE
                     RMRLPVVLFAEGGGGRPGDTDMPVVAGLDTRAFEHFARLSGLVPSVGIAVGRVFAGNA
                     ALLGCCDTIIATADSNIGMGGPAMIEGGGLGTFTPEDIGPIGTQVANGVVDLAVADEA
                     GAVAVARRYLSYFQGTVGGGAASDQALLRDVVPEQRKRVYDVRRVIDGLADQGSVLEL
                     RAGYAPGIITALVRLDGLPVGLLANDPAVLGGAIDAPAADKAARFLQLCDGHDLPVLS
                     LCDTPGFLVGPEAEETAQVRRFSRLFVTAQSMTIPFVTVVLRKGYGLGAQAMAGGSFR
                     APLATLSWPTGEFGGMGLEGAVRLGFRRELEAIADPEERQAAFDSMVEAAYRHGQALN
                     MAAHLEIDDVIDPAETRGRVLHMFRSVPPPPPRTGRKRPGIDPW"
     gene            3168..>4508
                     /locus_tag="VNQ33_12690"
     CDS             3168..>4508
                     /locus_tag="VNQ33_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ99012.1"
                     /translation="MAHDLVIRSGRVVDGTGAPARTADIAVDDGRITAVGTVDGPGAR
                     ELDADGAVVAPGWVDVHSHYDGQATWDEELAPSSWHGVTTLVMGNCGVGFAPAAPDKH
                     DWLIGLMEGVEDIPGSALAEGITWEWESFPEYLDALEAKRWTTDLGAQIAHGAVRAYV
                     MGDRGARNEPATPDDIEAMAAIVKEAVAAGALGFSTSRTIAHRAIDGEPVPGTFAAED
                     ELFGIGRVLGELGTGVFELAPTGTAGEDIVAPMVEVAWMRKLADAIGRPVTFAMLQVD
                     AAPELWREMLDESAAAVSSGAPLHPQIAGRPTGLLSGFETTFSFIDGVPAYAALAGRS
                     AAEKAAYLRTAEGREAVVGWEPDDATAAQLAHAAERTYLLGDPPDYEPGPEAALAAVA
                     ARSGRSVVEVALDAMLANGGTGLLYLPILNYSDGNSDSTREMILHPAAVLGLADG"
BASE COUNT          609 a         1633 c         1666 g          600 t
ORIGIN      
        1 tgatcggcgg cagcggcgag aagaagacgc tgcggctcgt cgcccagtac gccgacgagt
       61 cgaacctcac ggcacccatc gaggaggtgc cccgcaagct cgacgcgctc gccgcccact
      121 gcgagcgcct cggccgggac cgctcggaga tcaccgtctc ggtgatgggc cgggcgtgtg
      181 tggccccgac ccacgaccag gccaaagcgg agctcgacgc cgccctgttg tcgcgcggcc
      241 tggacctgtc gtccatgtcg gaggaggacg ccgaggcgat gcgcagcctg atcccctacg
      301 gcgaccccga cgagatcgcc gagcacttct ccggctacct gatcgacggc gtcgacggcc
      361 tcacggtcag cgccatcgcc aacggccacc tcgagggccg ggtgtcgctc ctgggcgaga
      421 ccctgtccaa gctcttctag cccaggggga tcagctcgaa cgtggtggcc aggtcgactc
      481 ccaggcgcgg gccggcctgg gtcgccatgc cccggaggaa ctcgtcgggg ttcgtgccgg
      541 tggtcccctc ggcgagcgca tcgaggtaga cgcggtgggc ttcgagggag gcgatgcccc
      601 ggtcgagtgt gtcggtgatg tcgacgccgt gggtcgcttg gggcgatcca ccgaacagcg
      661 cgaagcggac cccgttccac ggtccgccgc ccgcaccgac gaacagccag cggttggcgg
      721 cgtcgcggac ggcatcgagc agggcgatcc cggtgttgcg gtggtctgcg tggttcaggc
      781 ccgggaagcc gaacgtctcg cggaagttga tcgagatgac gacctcgggc cggtgccggc
      841 ggatggcggc ggccagatcc cggcgcaggg cggtgttcgc ctcgacgagg ccgtcggggt
      901 gggcgaggaa ctcgacgacg tcgacgccga cggcggcggc cgaggcccgc tcctcgttct
      961 ggcggacgcg ggcgcacgca tcgggcgcca tcgagtcgat gccggcctcg cccgaggtga
     1021 ccaggcagta ggcgacggcc ttgccctggt cggtccagcg ggcgatggcc gatgccgcgc
     1081 cgtactcgag gtcgtcgggg tgcgccacca ccgccagggc gcgggaccag tcttcgggaa
     1141 cggcttccag ttcgggcatg gccccatcct cgcgtcgtgg tagtccttcc gcatgcctgc
     1201 gcccgagcct gccgtccacc gcctcgtcgc cccgatggcg gccacggtcg cgtcggtcgc
     1261 ggtggccgat ggcgaccacg tccgcgatgg ccagacggtg atcgtgctcg aggtcatgaa
     1321 gatggagcac ccgctccagg tcttcctgga cggcacgatc tcgggcctcg ccgtggcgga
     1381 gggcgacgcc gtcagccagg gtcaggagct cctcaagatc accgcggcgc ctgctccggg
     1441 cgccgctcgg gagccggcgg ccgtcgagga gacggcgccg accggcgagg agcgggccga
     1501 tctccgggag ctccgtcggc ggagggctct cctcgaggac gcggctcgac ccggcgccgt
     1561 cgcccagcgc cacggccgcg gccgcaggac ggcgcgggag aacgtcgctg acctgtgcga
     1621 tgacgggtcg ttcgaggagc acggcgggtt cgccttcgcc gcccaggagg ggcgacggga
     1681 tcgagccgac ctccaggcca acacgccggc ggacgggctc atcaccggca tcgggcgcgt
     1741 cgacggtgcg cccaccgcgg tcgtcgccta cgactacacg gtcctcgccg gcacccaggg
     1801 ccagcgcaac cacgccaaga aggaccgcat cttcgagctg atcgagcgga tgcgcctccc
     1861 ggtggtgctc ttcgccgagg ggggaggggg ccgccccggc gacaccgaca tgccggtcgt
     1921 cgccggactc gacacccggg cgttcgagca cttcgcccgg ctctcgggcc tggttcccag
     1981 cgtcggcatc gccgtgggcc gcgtcttcgc cggcaacgcc gcgctgctcg gctgctgcga
     2041 caccatcatc gctacggccg actcgaacat cgggatgggc ggcccggcga tgatcgaggg
     2101 cggcggcctc ggcacgttca cgcccgaaga catcggcccg atcggcaccc aggtggccaa
     2161 cggcgtcgtc gacctcgccg tggccgacga ggccggggcg gtcgccgtcg cgcggaggta
     2221 cctgtcgtac ttccagggca cggtcggcgg gggagcggca tccgaccagg cgctgctccg
     2281 cgatgtcgtg cccgagcagc gcaagcgggt gtacgacgtg cgccgcgtca tcgacggatt
     2341 ggccgaccag ggaagcgtgc tcgagctgcg ggcgggctat gcgccgggga tcatcaccgc
     2401 gctggtgcgg ctcgacgggt tgccggtcgg gctcctggcg aacgacccgg ccgtgctggg
     2461 cggggcgatc gatgcgccgg cggccgacaa ggcagcccgc ttcctccagc tgtgcgacgg
     2521 tcacgacctg ccggtgctct cgctgtgcga cacgccgggg ttcctggtcg ggccggaggc
     2581 cgaggagacc gcgcaggtgc ggcggttctc ccgcctgttc gtcaccgccc agtcgatgac
     2641 gatcccgttc gtcacggtgg tgctccgcaa gggctacggg ctgggcgccc aggcgatggc
     2701 cggcggctcg ttccgggctc cgctcgccac gctcagctgg ccgaccggtg agttcggcgg
     2761 catggggctg gagggagcgg tgcggctcgg gttccggcgc gagctcgagg ccatcgccga
     2821 ccccgaggag cggcaggcgg cgttcgactc gatggtcgag gccgcctacc gccacggcca
     2881 ggccctgaac atggccgccc acctcgagat cgacgacgtg atcgatcccg ccgagacgcg
     2941 cggccgcgtc ctccacatgt tccggtcggt cccgcctccg ccaccgcgga cgggccgcaa
     3001 gcgccccggc atcgacccct ggtaggcagg gcggcccgat ccgagcgtgc gaccggtcgg
     3061 tgcgccaacc gggttgtggt cgagcgagca cgcgcggcgt cggatttgta ccgtgttctc
     3121 gttcggcgcc cctccctccg gcgcccggac cacaaggggg caacggcatg gcacacgatc
     3181 tggtcatccg cagcggccga gtcgtcgacg ggaccggtgc gccggctcgc accgccgaca
     3241 tcgccgtcga cgacgggcgc atcaccgccg tgggcacggt ggacggcccg ggggcccggg
     3301 agctcgatgc cgacggcgcc gtggtggcac ccggttgggt cgacgtgcac agccactacg
     3361 acggccaggc cacctgggac gaggaactgg cccccagctc gtggcacggc gtcaccacgc
     3421 tcgtgatggg caactgcggg gtcggcttcg cccctgccgc gcccgacaag cacgactggc
     3481 tgatcgggct gatggagggc gtcgaggaca tcccgggctc ggcgctggcc gaggggatca
     3541 cctgggagtg ggagagcttc cccgagtacc tcgacgcgct cgaagccaag cgctggacca
     3601 ccgacctcgg cgcccagatc gcccacgggg cggtacgggc ctacgtgatg ggcgatcgtg
     3661 gcgcccgcaa cgagccggcc acgcccgacg acatcgaggc catggccgcc atcgtcaaag
     3721 aagccgtcgc cgccggtgcg ctcggcttct ccaccagccg gaccatcgcc caccgcgcca
     3781 tcgacggcga accggtgccc ggcacgttcg cggccgaaga cgagctgttc ggcatcggcc
     3841 gggtgctcgg tgagctcggt accggcgtgt tcgagctggc gcccacgggc accgccggcg
     3901 aggacatcgt cgccccgatg gtggaggtgg cctggatgcg caagctggcc gacgccatcg
     3961 ggcgccccgt caccttcgcc atgctccagg tggacgccgc gcccgagctg tggcgggaga
     4021 tgctggacga gtcggcagcc gccgtgtcca gcggggcgcc gctccacccg cagatcgcgg
     4081 ggaggcccac cggcctgctg tcggggttcg agaccacgtt ctcgttcatc gacggcgtac
     4141 ccgcctacgc cgccctcgcg ggccggtccg cggccgagaa ggcggcgtac ctgcgcaccg
     4201 ccgaggggcg ggaggccgtc gtcgggtggg agcccgatga cgccaccgcc gcccagctcg
     4261 cccacgccgc cgagcgcacc tacctgctgg gcgacccgcc cgactacgag cccggccccg
     4321 aggccgcgct cgccgccgtg gccgcccgga gcgggcgctc cgtggtggag gtggcgctcg
     4381 acgccatgct cgccaacggc ggcaccggcc tgctctacct gccgatcctc aactacagcg
     4441 acggcaactc ggactcgacc cgggagatga tcctgcaccc cgccgccgtc ctcggcctcg
     4501 ccgacggc
//
LOCUS       DATMFS010000664         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_139291, whole genome shotgun sequence.
ACCESSION   DATMFS010000664 DATMFS010000000
VERSION     DATMFS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139291"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VNQ33_12695"
     CDS             <1..667
                     /locus_tag="VNQ33_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ99013.1"
                     /translation="AGRRAPPGRRALMPRVTGGSVAAHRADTTARIFASLRDLLYERG
                     YDALSLADVAEHAGMARTAMYNYFSDKEALLGAYTAQETDVFLAQLDEALRNVDNPVD
                     QLRVFVRLQVEYFQDSHLPPGPTLRLLLPEPAATEVIGHIALLEARLHQILRVGRDRR
                     YFLVDDVDASTAMITACVSRGSVADPERTTEEAVAATEAFVLRALGAKFAADGRPRRL
                     PRR"
     gene            complement(843..2732)
                     /locus_tag="VNQ33_12700"
     CDS             complement(843..2732)
                     /locus_tag="VNQ33_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HWJ99014.1"
                     /translation="MYRPTRLRLARTTKRPAGRALLALALAAVVAVPALVLADSGPAF
                     AAGSVSLSKSTDVTASGETITVNGSGFDSTRGIYAMFCKQVGTSGTAAGRASGDNCAS
                     TQFWITGPGAGVPPSGTTAWTGTGTFEISFPVAAGFKAIDCRATGVVCGIQTRNDHRE
                     PGVYDQDTFTPITFAPDAPTGPSATVTPTTGLDAAGTTVTVAGIGYPDGQGVYIRLCK
                     APTGAIGTEAGRPAAGDCDGQGLWASPTPPDPTLPVITDGAFSVELDVAGAFAGSPAN
                     IDCMTAGSCGVFIRRDHNGGAPDYGLDSFIPISFDPATTPPVIEEPEEPSLNDVTVTL
                     SKSTGLLNNQVITVTGEDFVANQGVYVQFCAAPTGQLGTAAGRATSCYPEQDNVHTVW
                     VSTIAGDGTFSTPLTVVSSFTDADGNLVDCTVDGACGVFVRRDHNGGTSDYSQDAFVP
                     VTFGDGSVVPGPDPTIGASKTTGLDRVGDKVRVKGANFQPGTELFVALCDANVANFAA
                     CDFDNVQQVTVDDLGTFMTDLHVRAAFGDVNCLAEGTACAIQTWAVSGGDGSEEVTLP
                     VSFASETTSTTTTPGTTDTPDSVDDGTLPRTGSQTAGLAVAGLLALGVGTAMLTTSRR
                     RRATN"
BASE COUNT          410 a          987 c         1068 g          404 t
ORIGIN      
        1 cgctgggcgt cgagccccgc caggccggcg agcactgatg ccccgggtga ccggcggctc
       61 cgtcgccgcc caccgcgccg acaccaccgc ccggatcttc gcctcgctcc gagacctgct
      121 ctacgagcgg ggctacgacg ccctgtcgct ggccgatgtc gccgagcacg ccggcatggc
      181 ccgcacggcg atgtacaact acttctcgga caaagaggcc ctcctcggtg cgtacaccgc
      241 ccaggagacc gacgtcttct tggcccagct cgacgaggcc ctgcgcaacg tcgacaaccc
      301 ggtggaccag ctgcgggtgt tcgtccgcct ccaggtcgag tacttccaag acagccacct
      361 cccgcccggc ccgacgctcc gcctcctgct ccccgagccg gccgctaccg aggtcatcgg
      421 ccacatcgcc ctgctggagg cccgcctcca ccagatcctc cgggtcggcc gtgaccgccg
      481 ctacttcctg gtcgacgacg tcgacgcctc cacggcgatg atcaccgcct gcgtcagccg
      541 aggcagcgtc gccgaccccg agcgaaccac cgaagaagcc gtcgccgcca cggaggcgtt
      601 cgtgttgcgg gccctgggcg ccaagttcgc cgcggacggc cgcccgaggc gcctcccccg
      661 ccgctgaccc cacctcgtgc cgggcgtcgc ctcgcgcggg gcgggctcgg acatcagcag
      721 cacctcccac cggtttcggg tcgtctgtgc acctcagcgg tgcacgatcg acccgaaatg
      781 gcgtcgaccg ggccgggcgc tggcgcgccc gacccggtct cagctctctt ggacgcggtg
      841 cgtcagttcg tcgcccggcg gcggcgggac gtggtcagca tcgcggtgcc gactcccagg
      901 gccaggaggc cggcgaccgc caggccggcg gtctggctgc ccgtccgggg gagggtgccg
      961 tcgtcgacgg agtccggcgt gtccgtcgtg ccgggggtgg tcgtggtgct cgtcgtctcc
     1021 gaggcgaagc tcaccgggag ggtgacctcc tcggagccgt cgccgccgga gaccgcccag
     1081 gtctggatgg cacaggcggt gccctcggcc aagcagttca cgtcgccgaa ggcggcccgg
     1141 acgtggaggt cggtcatgaa ggtgccgagg tcgtcgacgg tgacctgctg gacgttgtcg
     1201 aagtcgcagg cggcgaagtt cgcgacgttc gcatcgcaca gggcgacgaa cagctcggtg
     1261 ccgggctgga agttcgcgcc cttgaccctg accttgtcgc cgactcgatc caggccggtg
     1321 gtcttcgacg caccgatggt gggatcggga ccgggcacga ccgagccgtc accgaacgtg
     1381 accggcacga aggcatcctg cgagtagtcg ctggtgccgc cgttgtggtc gcgacggacg
     1441 aacacgccgc aggcaccgtc gacggtgcag tccaccaggt tgccgtcggc gtcggtgaac
     1501 gacgagacga cggtgagcgg cgtgctgaac gtgccgtcgc cggcgatcgt gctgacccag
     1561 accgtgtgga cgttgtcctg ctccgggtag cacgaggtgg cccggccggc agcggtgccg
     1621 agctggccgg tgggcgccgc gcagaactgg acgtacacgc cctggttggc cacgaagtcc
     1681 tcgccggtga ccgtgatgac ctggttgttc aggaggccgg tggacttgga gagcgtgacg
     1741 gtgacgtcgt tcagggacgg ctcctcgggc tcctcgatga ccggcggggt ggtggccggg
     1801 tcgaacgaga tcgggatgaa cgagtccagc ccgtagtcgg gggcgccgcc gttgtggtcc
     1861 cggcggatga acacgccgca gctgccggcg gtcatgcagt cgatgttcgc gggcgaaccg
     1921 gcgaaggcgc cggccacgtc gagctcgacg ctgaaggctc cgtcggtgat gacgggaagc
     1981 gtcgggtccg gcggggtcgg agaggcccag aggccctggc cgtcgcagtc accggcggcg
     2041 ggacggcccg cctcggtccc gatggcgccg gtcggcgcct tgcacaggcg gatgtagaca
     2101 ccctggccgt cggggtagcc gatgccggcc acggtgaccg tggtcccggc cgcgtcgagg
     2161 cccgtcgtcg gcgtcaccgt cgcggaggga ccagtgggcg cgtcgggggc gaaggtgatc
     2221 ggcgtgaacg tgtcctggtc gtagacaccg ggctcacggt ggtcgttgcg ggtctggatg
     2281 ccgcacacca ccccggtggc ccggcagtcg atcgccttga agccggccgc gacggggaac
     2341 gagatctcga aggtccccgt gccggtccaa gccgtggtgc ccgacggcgg gacgcccgcc
     2401 cccggaccgg tgatccagaa ctgggtgctc gcgcagttgt caccggacgc acgcccagca
     2461 gcggtgcccg acgtccccac ctgcttgcag aacatggcgt agatgccacg ggtcgagtcg
     2521 aagcccgaac cgttcacggt gatcgtctcc cccgaggcgg tgacgtcggt cgacttcgac
     2581 agcgacaccg agccggccgc gaacgccgga ccggagtcgg cgaggacgag cgccgggacg
     2641 gcgacgacgg cggccagggc gagggccagc aaggcgcgac ccgccggtcg cttggtagtg
     2701 cgggcgagcc gcaggcgggt gggacggtac atgttcaggt ctcctagcgg gaaggtgcag
     2761 ggattcgggg cggggtggag atcaggactc gggggcggcc ttgcggctgc ggaagaacaa
     2821 gaagccgccg atgcccgccg cggccaggac ggcgacgatc aggatcggg
//
LOCUS       DATMFS010000665         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_253537, whole genome shotgun sequence.
ACCESSION   DATMFS010000665 DATMFS010000000
VERSION     DATMFS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253537"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VNQ33_12705"
     CDS             complement(<1..611)
                     /locus_tag="VNQ33_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ99015.1"
                     /translation="MPRLRQVPRAEAPDGIVPVMYDFIFGDRDPVAEPGLGNGTPGNW
                     WTVVAQSPELMEHCVSGFAFYRSDDRELPKELRELAQTRAGWARGSRFVFSQHCKASR
                     DNGVSEEKIRAVPSWETSDVYSPAERAVLAWTDALVLHGGRASDGTFAALREHLSELA
                     ILELTYITCWYDLHAVMSRALRLELDDVDDPITELTGPGITRSL"
     gene            770..>2246
                     /locus_tag="VNQ33_12710"
     CDS             770..>2246
                     /locus_tag="VNQ33_12710"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWJ99016.1"
                     /translation="MESRRTTLIGGAIVVVLVLLAAASVWFKVTSSGDEAQRPGMKKG
                     DPAAEAADAFAQKWAEGRLSDVAFVSDDGDVNALSLAITAGLGSGAAGKPAKVEVTSL
                     TEVPAGKDGFPRVSAALRITWQVDPARSWTYDSAVELVKSTTPAGEETWLVDWTPAVV
                     HKRLVEGDTLQTVRLPAARGEILGPDGTPLVGLRPVVAIGIQPGATTDRTSAAQQVAR
                     LVGVDSAALADRVQAGGDTDLISVITLRKEAFDPISAQLKAIPGVVLREDEIPLAPTR
                     DFARALIGTVGPATAEIAAASDGRIAEGDLTGLSGIQASQDVALAGQAGLSIRVLPAE
                     GSGREAYVLKDFPTKSGESVTVTLDPKVQAAADEVMAAAPKPAALVAIRASTGDVLAV
                     SNGPEGADGYNRALIGKYPPGSTFKIASALALLENGLTPETTVDCPATIVVGKEFRNA
                     EGEVLGSVPFSKDFADSCNTAFVGQSRTITVQQLADTAQKLG"
BASE COUNT          320 a          819 c          784 g          323 t
ORIGIN      
        1 agcgaccgcg tgatgcccgg tccggtcagc tcggtgatcg ggtcgtcgac gtcatcgagc
       61 tccaggcgca gcgcccggct catcaccgcg tggaggtcgt accagcaggt gatgtaggtg
      121 agctccaaga tggccagctc cgagaggtgc tcgcggaggg cggcgaaggt gccgtcggag
      181 gcccggccgc cgtggagcac gagggcgtcg gtccacgcca gcacggcccg ttcggccggc
      241 gagtacacgt cgctcgtctc ccacgacggg acggctcgga tcttctcctc gctcacgccg
      301 ttgtcccggc tggccttgca gtgctgcgag aacacgaagc ggctgccgcg ggcccagccg
      361 gcccgggtct gggccagctc ccgcagctcc ttgggcagct cccggtcgtc ggaccggtag
      421 aaggcgaacc cgctcacgca gtgctccatc agctccgggc tctgggccac caccgtccac
      481 cagttgcccg gcgtcccgtt gccgagcccc ggttcggcca cggggtcgcg gtcgccgaag
      541 atgaagtcgt acatgacggg cacgatcccg tcgggggctt cggctctggg tacctggcgc
      601 agtctgggca cgggggcggc tcctcgatcg atccgtcgcg tggccgggac cgtacgccgc
      661 gtgggcggtc tcgtcatcgg atcgcgcgca ggttggaacc gaaagaccca ggcggaccgg
      721 tggagccgat cggtaggttg ccctggctgg acacgatgag aggtgaaggg tggagagcag
      781 gcgcacgacg ctgatcggcg gggcgatcgt ggtggtcctg gtgctgctgg ctgcggcgtc
      841 ggtctggttc aaggtgacca gctccggcga cgaggcccag cgtcccggga tgaagaaggg
      901 cgatcccgcg gcagaagcgg ccgacgcctt cgctcagaag tgggccgagg gcaggctcag
      961 cgacgtcgcc ttcgtctcgg acgacggcga tgtcaacgcc ctctccctcg ccatcaccgc
     1021 cgggctcggt tcgggcgccg ccggcaagcc ggccaaggtc gaggtcacct cgctcacgga
     1081 ggtcccggct ggcaaggacg gcttccctcg cgtgtctgct gcgctccgca tcacctggca
     1141 ggtcgacccg gcccggtcct ggacgtacga ctcggcggtg gagctggtca agagcacgac
     1201 acccgcgggt gaggagacct ggctcgtcga ctggacgccg gcggtggtgc acaagcgcct
     1261 cgtcgagggc gacaccctcc agaccgtccg cctgcctgcc gcccgcggcg agatcctcgg
     1321 ccccgacggc acgcccttgg tgggcctgcg accggtcgtg gcgatcggca tccagcccgg
     1381 tgccaccacc gaccggacct cggctgcgca gcaggtcgcc cgcctcgtgg gcgtcgactc
     1441 cgccgcgctg gccgatcggg tccaagccgg gggcgacacc gacctcatct ccgtcatcac
     1501 cctccggaag gaggcgttcg atcccatcag cgcccagctg aaggcgattc cgggcgtcgt
     1561 gctccgagag gacgagatcc ccctggcccc cacccgagac ttcgcccgcg ccctcatcgg
     1621 caccgtcggc ccggccaccg cggagatcgc ggccgcctcc gacggccgca tcgccgaggg
     1681 cgacctcacc ggtctctccg gcatccaggc gtcgcaggac gtcgccctcg ccgggcaggc
     1741 cggtctgtcg atccgggtcc tgcccgccga gggctccggt cgcgaggcgt acgtgctcaa
     1801 ggacttcccc acgaagtccg gcgagtccgt caccgtcacc ttggacccga aggtgcaggc
     1861 cgccgccgac gaggtgatgg ccgccgcgcc caagccagcg gccctcgtcg ccatccgggc
     1921 cagcaccggc gacgtgctcg ccgtctccaa cggacccgag ggtgccgacg gctacaaccg
     1981 ggcgctgatc gggaagtacc cgcctggctc caccttcaag atcgcgtcgg ccctcgcgct
     2041 gctcgagaac gggctgaccc cggagaccac cgtcgactgc ccggccacca tcgtcgtcgg
     2101 caaggagttc cgcaacgccg agggcgaggt gctgggctcg gtgcccttca gcaaggactt
     2161 cgccgattcg tgcaacaccg ccttcgtggg ccagtcccgg accatcaccg tccagcagct
     2221 ggccgacacc gcccagaagc tcgggt
//
LOCUS       DATMFS010000666         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_253764, whole genome shotgun sequence.
ACCESSION   DATMFS010000666 DATMFS010000000
VERSION     DATMFS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253764"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VNQ33_12715"
     CDS             <1..487
                     /locus_tag="VNQ33_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99017.1"
                     /translation="RYYANLIGGSAKVGNVVGLAPSNHATTVSGLLTLGRFIGAVDPI
                     FAATNWIGLPSLQQQADPGSTYFTNLNGSGETRPGLRYVNIATKYDEVVTPYRQAFIT
                     AGSGATVQNITLQDVCKADLTDHLGIVYDTNVYQLVLNALEPGDQRPIACSTSLPLFG
                     T"
     gene            859..1644
                     /locus_tag="VNQ33_12720"
     CDS             859..1644
                     /locus_tag="VNQ33_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ99018.1"
                     /translation="MPLATTTHPADPATAPRRAGRPVAARTVSAVKVYGTGGTEVRAL
                     DGVTVDVPAATFTAVMGPSGSGKSTLMHCLAGLDTLTSGHVFVGDQELGALSDKELTR
                     VRRERIGFVFQAFNLLPTLSAGENITLPMDLARQKPDAEWLEKVIATVGLQDRLHHRP
                     SELSGGQQQRVAVARALASKPEVVFADEPTGNLDRRSGTEVLSFLREAVDRFGQTVVM
                     VTHDPVAASYADSVLFLADGQIVDQLADPTATAVLDRMKALGD"
     gene            1659..>2608
                     /locus_tag="VNQ33_12725"
     CDS             1659..>2608
                     /locus_tag="VNQ33_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ99019.1"
                     /translation="MTLQGLWARKRRVIGTATAVVLGVAFLSATLVLGDTLDASFGTV
                     FRQANAGTDVVVRSAASINSDQGRQRATLDATVVDQVAAVPGVAEAVGEVTGTAQILG
                     SDGSPIGGQGPPTEASAWIDDPDLNPYHVTSGRAPARAGEAVIDANSASDGGISVGDT
                     ATIRTPDPVEVDVVGIVGFGDETSMAGATYVGLTPDQAVDLLGRAGQVTDVRVRGDGS
                     VSDTQLRDAVAEVVPDKAEAITGAQLTAEQQEDIQGDFLGFLKNFLLAFAGVALVVAT
                     FSIANTFTILSAQRSRESALMRAIGAGRGQVLAIALIEAVV"
BASE COUNT          379 a         1016 c          843 g          370 t
ORIGIN      
        1 ccggtactac gccaacctca tcggcggatc ggccaaggtg ggcaacgtgg tcgggctcgc
       61 cccgtcgaac cacgccacga cggtcagcgg actgctgacc ctcggccgct tcatcggcgc
      121 cgtcgatccg atcttcgccg ccaccaactg gatcgggctc ccctcgctcc agcagcaggc
      181 cgacccgggc tcgacctact tcacgaacct caacggcagc ggcgagaccc gtcccggcct
      241 ccgctacgtg aacatcgcca cgaagtacga cgaggtcgtc accccgtacc gccaggcctt
      301 catcaccgcc ggctccggcg ccaccgtgca gaacatcacc ctgcaggacg tgtgcaaggc
      361 ggacctcacc gaccacctcg gcatcgtcta cgacaccaac gtgtaccagc tggtcctcaa
      421 cgccctcgag cccggcgacc agcgccccat cgcctgctcc accagcctgc ccctcttcgg
      481 cacctgaggc ttcgcctcag gcaccggatg tccggctccc agtccaggtt gcagatccac
      541 ctggcatcgc cgctcacggc tcctctcccc gacttcgtcg ttgagcgacc catgccgtcc
      601 tctccgctcg ggtgctcccg gccctgacgg gcctccagca ccgggccgct cgggcccttc
      661 gccttcggcg gtccttggct gtcatccgtt gctggcgccg ggtgtccgtc ggacgtgatc
      721 aaccaggctg gccgcttggg gtaggggtag ccccacctgg gagacaccgg gcggccccat
      781 tccggggctg accggcgatc cgtaccttcg aacgggcagc acgaacgctg cccgcacgac
      841 gacgaaggga cgcggtcgat gccgctcgca accaccaccc acccggccga tccggccacc
      901 gctccccgcc gtgcgggccg accggtggcc gcccgcacgg tcagcgccgt caaggtctac
      961 ggcaccggcg gcaccgaggt gcgggcgctc gacggcgtga ccgtcgacgt cccggccgcc
     1021 accttcacgg cggtgatggg cccgtccggt tcgggcaaga gcaccctgat gcactgcctc
     1081 gccggcctcg acacgctgac cagcggccac gtgttcgtgg gcgaccagga gctgggcgcc
     1141 ctgagcgaca aggagctgac ccgggttcgt cgggagcgga tcgggttcgt gttccaggcg
     1201 ttcaacctgc tgcccaccct ctccgccggc gagaacatca ccctgccgat ggacctggcc
     1261 cgccagaagc ccgacgccga gtggctggag aaggtcatcg ccaccgtcgg cctccaggac
     1321 cggctgcacc accggccgag cgagctgtcc ggcggccagc agcagcgggt cgccgtggcc
     1381 cgggcgctgg cgagcaagcc cgaggtcgtc ttcgccgacg agcccaccgg caacctcgac
     1441 cgccgcagcg ggaccgaggt cctctcgttc cttcgcgagg cggtcgaccg cttcggccag
     1501 accgtggtga tggtcacgca cgatccggtc gctgcgtcct atgccgacag cgtgctgttc
     1561 ctggccgacg gccagatcgt cgaccagctg gccgatccca ccgccaccgc ggtgctcgac
     1621 cgcatgaagg cgctggggga ctgaccgtgc tgttccggat gaccctccag ggcctgtggg
     1681 cccgcaagcg gcgcgtgatc ggcacggcca ccgccgtcgt gctcggcgtc gccttcttgt
     1741 cggcgacgct ggtgctgggc gacacgctcg atgccagctt cggcaccgtc ttccgccagg
     1801 ccaacgccgg caccgacgtc gtcgtgcgca gcgccgcgtc gatcaacagc gaccagggcc
     1861 gccagcgggc gacgctcgac gcgaccgtgg tcgaccaggt cgcggcggtt cccggcgtgg
     1921 ccgaggcggt cggcgaggtc acgggtacgg cccagatcct cggttccgac ggctccccca
     1981 tcgggggcca gggcccgccc accgaggcga gcgcctggat cgacgacccc gacctcaacc
     2041 cataccacgt cacctccggc cgggctccgg ccagagccgg cgaagcggtc atcgacgcca
     2101 acagcgcatc ggacggcggc atctcggtcg gcgataccgc cacgatccgg acgccggacc
     2161 ccgtcgaggt cgacgtcgtc ggcatcgtcg gcttcggcga cgagacgtcg atggccggtg
     2221 ccacctacgt cggcctcacc ccggaccagg ccgtcgacct gctgggcaga gccggccagg
     2281 tcaccgacgt gcgggtccgc ggcgacggct cggtgtccga cacccagctg cgcgacgccg
     2341 tggccgaggt ggtgcccgac aaggccgagg ccatcaccgg tgcccagctc acggccgagc
     2401 agcaagagga catccagggc gacttcttgg gcttcctcaa gaacttcctc ctggcgttcg
     2461 ccggcgtcgc cctcgtggtc gccaccttca gcatcgccaa cacgttcacg atcctgagcg
     2521 cccagcgcag ccgggagtcc gccctcatgc gggcgatcgg cgccggtcgg ggccaggtcc
     2581 tcgccatcgc cctcatcgag gccgtcgt
//
LOCUS       DATMFS010000667         5317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_23206, whole genome shotgun sequence.
ACCESSION   DATMFS010000667 DATMFS010000000
VERSION     DATMFS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23206"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VNQ33_12730"
     CDS             complement(<1..568)
                     /locus_tag="VNQ33_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99020.1"
                     /translation="MTTDPEGHSDVMAITFEGDAAKETEAGANAAAEGYLSARKASAK
                     ATTDKALTNLNDDIVAANKAVEEATGAVNAAPAGSTERTQAQARLQAANSALTQLQAQ
                     QTKIRQFDPDAVGIITRKASVADPVTSKMALGKAVGVFGLFVLGGLAAAWLLDHRDGL
                     GGGRRRVEALLPGASTRLMPGAEGGHATP"
     gene            complement(565..882)
                     /locus_tag="VNQ33_12735"
     CDS             complement(565..882)
                     /locus_tag="VNQ33_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWJ99021.1"
                     /translation="MSELESGFKIGDVVAMVVRRWPVVVGSAVIGLIAGYLVFAAAPT
                     TYSATARIQVLAFDDAFSDTKSAEVDMSTERDLVKSDAVAEAIRTKVASTGRTGRSSA
                     TSR"
     gene            complement(929..2473)
                     /locus_tag="VNQ33_12740"
     CDS             complement(929..2473)
                     /locus_tag="VNQ33_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWJ99022.1"
                     /translation="MSDATGSAPAPTAPESVGAVARGGALNMAGSVIYGTANFILLVV
                     LNRVLGTAEAGIVLVAIAIFNIIETVAELGCGTGLVRMLSRDRAIGRQDLLRATVIVA
                     IVPVAAASVVATAVLWLLAEPLARLVADKGQVQLVADVLRAMSLFLPFATLHSVLVSG
                     TRGFNTMIPQTAIERVGRASTLPVVVAIAVGLGMGPVGVGACWAATTVVALVFSSRAM
                     AKRVKQATANKGLTPVPPSRAIARDFWAFTAPRAVGQSAEVAVNWIDTVLVGVLASTT
                     AAGIYASGTRYILPGVYASDALMQVTGPRISGLLSKGNKTEASQLIKVVAGWQSAVTW
                     PLYLVIALFPSPLLRVFGPEVVEAKGAIVAISVAMLVTAPAGPVASVILMAGRSRQAM
                     FNTLILVVVNVGGNLLFVPSHGITAAGATWGVTILVAALLPGWQAARSVGVSTLGRPA
                     LVAVAAAAATIGVIGLGARLWLGDGTSGLLVTGSLGLLAYLVALMAVRAELHLDALWN
                     GIRRRS"
     gene            complement(2470..3654)
                     /locus_tag="VNQ33_12745"
     CDS             complement(2470..3654)
                     /locus_tag="VNQ33_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99023.1"
                     /translation="MKPSARHASSLEALTELLDLRPTSGADRTRLGVVAGRSGPRFLV
                     PLDFPEASPQACWAYLGLRDRKTQLARGAVGLALRTGLHRLVVRDDLTADTGPGSLIA
                     LLEELLEDPEEEPGLAIAAGLSRIDEVWKPTLQVFRPDGTPAAFVKIGLGPVGDRLVG
                     TEAETLAAWADHPDPRLVVPDLIADARWNGLRIAVVAPLPDDARRLPPGATSAWAVRA
                     LDGPEEHGPLTTAPWWTGRRRAWADEPAVDALLDAIEQRHGGSDRAWARWHGDWVPWN
                     LARCHRGLVAWDWEYSEPGAPVGLDEVHGAYQQARVVEGRSIADSLLVARTTAHHLGA
                     RMTTGMDAAVHHEGAAARWLSDAHVAMLVTRSAELERLTGEPPAHQPELLAAARDAVA
                     VP"
     gene            complement(3782..5305)
                     /locus_tag="VNQ33_12750"
     CDS             complement(3782..5305)
                     /locus_tag="VNQ33_12750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWJ99024.1"
                     /translation="MLRRMLPLLASAAVVVGLAVPASARAADPPPVQTHIVGGSVAPA
                     GSYGWTAALIRRGASRTAGFRCGAVVLSRSWVLTAGHCAIDYDNAYPDSVYGNYVAPS
                     FLDVLASTQQLSGTSGQLVHVAKIYVHPSYNVATSDYDVALLRLARPISAEEIAVIGT
                     TASELPLDDAGTTAIVSGWGTTSSGGSISTLLRSVGVPIQSNTTCGNAYYPGFTADGE
                     VLEYHASNMICAGPLGGGKDSCQGDSGGPLAVQAPDSSWRVVGTVSFGLGCARPGYPG
                     VYQRLTATSPWIANTRRFGPFNPDGSAYVVQQYRDFVGRSPNSTELSSWVTKLRSTPA
                     ATLITSLQASPAWDANAGMNTRLYRAAFLRNPDTSGLDFWVRKRWAGTSPVWIANYFT
                     ASSEFDRRYGSLSNDQFVTQIYQNVFGREPDPSGRAYWVNKLNGGTGRGRALYEWSNS
                     SEYRRKTDTPVRIITTRFGLLRAVPSPGEITASEPLNQRSLVDTLRTSYRYAARFNG"
BASE COUNT          721 a         1843 c         2045 g          708 t
ORIGIN      
        1 cgggggtggc gtggccgcct tcggccccgg gcatgagccg ggtgctggcg ccggggagca
       61 gggcttcgac ccggcgacgg ccgccgccga ggccgtcgcg gtggtcgagg agccaggcgg
      121 cggccaggcc gccgaggacg aacaggccga acacgcccac cgccttgccc agcgccatct
      181 tcgaggtgac gggatcggcc acgctggcct tgcgggtgat gatgccgacg gcgtcggggt
      241 cgaactggcg gatcttcgtc tgctgggcct ggagctgggt gagggcgctg ttggcggcct
      301 ggagccgcgc ttgggcctgc gtccgctcgg tgctgccggc gggggcggcg ttgaccgcgc
      361 ccgtcgcctc ctcgaccgcc ttgttcgccg cgacgatgtc gtcgttcagg ttggtcaggg
      421 ccttgtccgt ggtggccttg gcgctggcct tgcgggcgct caggtagcct tccgcagcgg
      481 cgttggcacc tgcctcggtc tccttggcgg cgtcgccctc gaaggtgatg gccatgacgt
      541 cggagtggcc ttcgggatcg gtggtcaccg tgatgtggcg gaggatcgcc cggttctccc
      601 cgtcgaggcc accttggtgc ggatggcctc ggccacggca tcggacttca ccaggtcgcg
      661 ctcggtcgac atgtcgacct ccgcgctctt ggtgtcgctg aaggcgtcgt cgaacgccag
      721 cacctggatc cgggcggtgg cgctgtaggt ggtgggcgcg gcggcgaaga ccaggtagcc
      781 ggcgatcagg ccgatgacgg ctgagcccac gaccaccggc cagcggcgga cgaccatggc
      841 gaccacgtcg ccgatcttga atccggattc cagttcgctc attcgatcct cgtgccgtcg
      901 tacggttgcc ggcgcagcaa ggtgcacttc aggatcggcg gcgaatgccg ttccacaagg
      961 catccaggtg gagttcggcg cgcacagcca tgagggcgac caggtacgcg agcagcccaa
     1021 ggctaccggt gaccagcagc ccggacgtgc cgtcgccgag ccacagccgg gcgccgaggc
     1081 cgatcacgcc gatggtcgcg gccgcggccg ccaccgccac gagcgccggc cggccgaggg
     1141 tcgagacccc cacggagcgg gcggcctgcc agccggggag gagcgcggcg acgaggatgg
     1201 tcacgcccca ggtcgccccc gcggcggtga tcccgtggct cgggacgaag agcaggttgc
     1261 cgcccacgtt gacgaccacg aggatcagcg tgttgaacat ggcttggcgg ctgcggccgg
     1321 ccatcaagat gaccgaggcg acgggcccgg ccggggcggt cacgagcatg gcgaccgaga
     1381 tggcgacgat ggcgcccttg gcctcgacca cctcgggccc gaacacgcgc agcagcggcg
     1441 acgggaacag ggcgatgacc aggtacagcg gccacgtcac cgccgactgc cagccggcca
     1501 ccaccttgat cagctgcgac gcctcggtct tgttcccctt cgagagcagg ccgctgatcc
     1561 gcggtcccgt gacctgcatc agggcgtcgg aggcatagac cccggggagg atgtagcggg
     1621 tgccggacgc gtagatgccc gcggcggtgg tggaggcgag gacgcccacc agcaccgtgt
     1681 cgatccagtt gacggcgacc tcggcggatt gcccgaccgc ccgcggcgcg gtgaacgccc
     1741 agaagtcacg ggcgatggcc cgcgagggcg gcaccggggt gagtcccttg ttggccgtcg
     1801 cctgcttgac ccgcttggcc atcgcccgcg acgagaacac cagggccacc acggtggtcg
     1861 ccgcccagca cgccccgaca ccgaccggcc ccatgcccag gccgacggcg atggccacga
     1921 cgaccgggag cgtcgacgcc cgcccgaccc gctcgatggc ggtctggggg atcatggtgt
     1981 tgaacccgcg cgtgcccgac accagcaccg agtggagcgt ggcgaacggg aggaacaggg
     2041 acatggcgcg cagcacgtcg gccaccagct ggacctgccc cttgtcggcc acgaggcggg
     2101 cgaggggctc ggcgaggagc cagagcacgg ccgtcgcgac caccgaggcc gccgcgacgg
     2161 ggacgatcgc cacgatcacc gtcgcccgca gcaggtcctg gcggccgatg gcgcggtcgc
     2221 ggctgagcat ccggaccagg ccggtgccgc agcccagctc ggccacggtc tcgatgatgt
     2281 tgaagatggc gatggcgacc agcacgatgc cggcctcggc ggtgccgagc acccggttga
     2341 gcacgacgag caggatgaag ttggccgtgc cgtagatgac cgagccggcc atgttgaggg
     2401 cgccgccgcg cgcgacggca cccacgctct cgggtgccgt cggcgccggg gccgacccgg
     2461 tcgcgtcgct cacgggacgg ccaccgcgtc gcgggcagcg gcgagcagct cgggctggtg
     2521 cgcgggcggc tcgccggtca ggcgctccag ctcggcgctg cgcgtgacca acatggcgac
     2581 gtgggcatcg gacagccacc gtgccgcggc cccctcgtgg tggacggccg cgtccatccc
     2641 ggtcgtcatc cgtgcgccga ggtggtgggc ggtcgtccgg gcgacgagga ggctgtcggc
     2701 gatggaccgt ccctccacga cgcgggcttg ctggtaggcc ccgtggacct cgtcgaggcc
     2761 cacgggggca ccgggctcgg agtactccca gtcccaggcg accaggccgc ggtggcagcg
     2821 ggccaggttc cacggcaccc agtcgccgtg ccagcgggcc caggcccggt ccgagccgcc
     2881 gtggcgctgc tcgatggcat cgaggagcgc gtcgaccgcg ggctcgtcgg cccacgcccg
     2941 acgccgcccg gtccaccacg gggccgtggt gagcgggccg tgctcctccg gtccgtcgag
     3001 cgcgcgcacg gcccacgccg acgttgcccc gggtgggagc cggcgagcgt cgtcagggag
     3061 cggggcgacc accgcgatgc gcagcccgtt ccagcgcgcg tcggcgatga ggtcggggac
     3121 cacgaggcgc gggtcgggat ggtcggccca cgcggcgagc gtctccgcct cggtccccac
     3181 gaggcggtcg ccgaccggcc cgagaccgat cttgacgaag gcggccgggg tgccgtcggg
     3241 ccggaacacc tggagcgtcg gcttccagac ctcgtcgatg cggctcaggc ccgcggcgat
     3301 ggcgaggccg ggctcctcct ccgggtcttc gagcagctcc tcgaggagcg cgatcaacga
     3361 cccggggccg gtgtcggcgg tgaggtcgtc gcgcaccacg agccggtgga gcccggtccg
     3421 cagggcgagg ccgaccgcgc cccgggcgag ctgggtcttg cggtcacgga gtccgaggta
     3481 cgcccagcac gcctggggcg acgcctccgg gaagtccagg gggacgagga accggggccc
     3541 ggaccgaccg gcgaccacgc cgaggcgggt gcggtccgcg ccactggtcg gccggaggtc
     3601 cagcagctcc gtcagggcct ccagcgacga cgcgtggcgt gcgctgggct tcaccggccc
     3661 ttcctgcaca ggtcggcgcg cccgatgcgc gaagccgtgg ccgcgccgac gcccgcgccc
     3721 gcggcggtcg cgccgctgac ccgcggccgt cgtctccccg agaagacctc ggtgtcgtcg
     3781 ctcacccgtt gaagcgggcg gcgtagcggt acgaggtgcg cagcgtgtcg accaggctgc
     3841 gctggttcag cggctcgctc gccgtgatct ccccggggct cgggacggcc cgcagcagcc
     3901 cgaaccgggt ggtgatgatg cgcacggggg tgtcggtctt gcgccggtac tccgacgagt
     3961 tggaccactc gtagagcgcc cggccccggc cggtcccgcc gttcagcttg ttcacccagt
     4021 aggcccggcc gctggggtcg ggttcgcgcc cgaagacgtt ctggtagatc tgcgtcacga
     4081 actggtcgtt cgacagcgag ccgtaccgcc gatcgaactc cgaggaggcc gtgaagtagt
     4141 tcgcgatcca gaccgggctc gtgccggccc agcgcttgcg cacccagaag tcgagaccgc
     4201 tcgtgtcggg gttgcgcagg aacgcggccc ggtacagccg ggtgttcatg ccggcgttgg
     4261 cgtcccaggc cggcgatgcc tggaggctgg tgatgagggt ggcggccggg gtgctgcgca
     4321 gcttggtgac ccacgagctc agctcggtgc tgttcggcga ccggccgacg aagtcccggt
     4381 actgctggac gacgtaggcg ctgccatccg ggttgaacgg gccgaagcgc cgggtgttgg
     4441 cgatccacgg cgacgtggcc gtgagccgct ggtagacgcc ggggtagccg ggccgggcgc
     4501 agccgaggcc gaacgagacc gtgcccacga cgcgccagga ggagtcgggc gcctgcacgg
     4561 cgagcgggcc gccggagtcg ccctggcacg agtccttccc accgccgagc ggaccggcgc
     4621 agatcatgtt cgacgcgtgg tactccagca cctcgccgtc ggcggtgaag cccgggtagt
     4681 aggcgttccc gcaggtggtg ttgctctgga tcggcacgcc cacggagcgg agcagggtcg
     4741 agatcgaccc gcccgacgac gtggtgcccc agcccgagac gatggccgtg gtgccggcgt
     4801 cgtcgagggg gagttccgag gccgtggtgc cgatgaccgc gatctcctcg gcgctgatgg
     4861 gccgggcgag gcggagcagg gcgacgtcgt agtcgctggt tgcgacgttg taggacgggt
     4921 ggacgtagat cttggccacg tggaccagct gcccgctcgt gccgctgagc tgctgcgtgc
     4981 tggccagcac gtcgaggaag ctcggcgcga cgtagttccc gtagaccgag tcggggtacg
     5041 cgttgtcgta gtcgatggcg cagtgcccgg cggtgaggac ccacgagcgc gacagcacga
     5101 ccgccccgca gcggaacccc gcggtgcggc tcgcaccccg ccggatcagc gccgcggtcc
     5161 atccgtagga cccggcgggg gcgacgctgc cgcccacgat gtgggtctgg accggtggcg
     5221 ggtcggccgc tcgggcgctg gccggcaccg ccagcccgac gaccaccgcc gcgctcgcga
     5281 gcagggggag catccgtcgc agcacggtgc cacgcta
//
LOCUS       DATMFS010000668         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_69413, whole genome shotgun sequence.
ACCESSION   DATMFS010000668 DATMFS010000000
VERSION     DATMFS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69413"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VNQ33_12755"
     CDS             complement(<1..553)
                     /locus_tag="VNQ33_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99025.1"
                     /translation="MSASPSSAVATDPPSLARIASLVHRATTDQPVARVVHLRVDHRD
                     ETFDLGFWDIPPVPSHPMEPLVGFVAPSSWDAIGLISTGRVRHLDEPGGGAAASLSTV
                     LLHRDGTAASVIAVEGAEPRLLDDPPEGLVPDVLNRVLQRPTPPPDAPTGALVELTWL
                     DRITTGLLRPRGRTRSWRWLADRH"
     gene            complement(727..1419)
                     /locus_tag="VNQ33_12760"
     CDS             complement(727..1419)
                     /locus_tag="VNQ33_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99026.1"
                     /translation="MGQLATIYGLDIETDNSEDGLDPAVASVRAVALSGQTFDELYVG
                     DEADLLRALDARLAGLPTGVVATWNGSAFDLPFLADRARILGIDLGLKLCLDRRLTLH
                     RAPLPGHAGAYRASWHEHGHLDTFRIYGDADGAAQRLSLRSLGRFVRIGSHDVHPART
                     QTLSNEALHACAPSDARLARVLAERRWPAAARMIDRVEWTEAEPVAVAAQRLARQDRT
                     VPRAMQPAIATS"
     gene            complement(1720..1884)
                     /gene="rpmG"
                     /locus_tag="VNQ33_12765"
     CDS             complement(1720..1884)
                     /gene="rpmG"
                     /locus_tag="VNQ33_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWJ99027.1"
                     /translation="MAKSDKRPIIKLKSTAGTGYTYVTRKNKTNTRERIELMKYDPKI
                     RQHVIFKEER"
     gene            2045..>2305
                     /locus_tag="VNQ33_12770"
     CDS             2045..>2305
                     /locus_tag="VNQ33_12770"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HWJ99028.1"
                     /translation="MRPIRSVRLLRALGILAFALLAALIPARAASAHAELIATDPADG
                     AVVDKAPEALTMQFSEGVSVRPDGIRVLDATGERVDAGAASAD"
BASE COUNT          316 a          742 c          918 g          329 t
ORIGIN      
        1 gatgccggtc ggcgagccat cgccacgagc gggtccgacc gcgcgggcgc aggaggccgg
       61 tggtgatgcg gtcgagccag gtcagctcga ccagggcgcc ggtgggggcg tcgggcggtg
      121 gggtcgggcg ctggagcacc cggttgagca cgtcgggcac caggccttcg ggcggatcgt
      181 cgaggaggcg gggctcggct ccttccaccg cgatcacgct ggcggcggtg ccgtcgcggt
      241 ggagcagcac ggtgctgagc gacgcggcgg ctcctccacc gggctcgtcg aggtggcgca
      301 cccggccggt gctgatgagg ccgatcgcat cccacgacga cggtgcgacg aacccgacca
      361 gcggttccat cgggtgcgag gggaccgggg ggatgtccca gaagcccaga tcgaaggtct
      421 cgtctcgatg gtccacgcgc aggtggacga cccgggcgac gggctggtcg gtggtggcac
      481 ggtgcaccaa cgaggcgatg cgggccagcg acggaggatc cgtggcgacg gcggaggacg
      541 gagatgcgga catgggcgga cctcgggagc agcgggaacg tttccggctg tccgggcccg
      601 gaggcagagg ggacggcgaa cctcgtggcg acagtgtgcc caccggggct gacggcggag
      661 gcccgatgct ccggaagtgg cgcaccggac gttgcggtgg cgacacggcg cccccgtcgg
      721 cgctgttcag gaggtggcga tggcgggctg catggcgcgg ggcaccgtgc ggtcctggcg
      781 ggcgaggcgc tgggcggcga ccgccaccgg ttcggcctcg gtccactcca cccggtcgat
      841 catccgtgcc gcggcgggcc accgccgctc ggcgagcacc cgggcgaggc gggcgtcgct
      901 gggggcgcag gcgtggaggg cctcgttgct cagggtctgg gtgcgcgccg ggtggacgtc
      961 gtgcgagccg atcctgacga agcggccgag cgagcgcagc gacagccgtt gcgccgcgcc
     1021 atcggcatcg ccgtagatcc ggaacgtgtc caggtgaccg tgctcgtgcc agctggcccg
     1081 gtaggcgccg gcgtggccgg gcaacggggc ccggtggagg gtcaggcgtc ggtccaagca
     1141 gagcttgagc ccgaggtcga tgccgaggat gcgggcccgg tcggccagga acgggaggtc
     1201 gaacgcactg ccgttccagg tggccacgac ccccgtcggg agaccggcga ggcgggcgtc
     1261 gagcgcacgg aggagatcgg cctcgtcacc gacgtagagc tcatcgaagg tctggcccga
     1321 caaggcgacg gctcggaccg aggcgaccgc gggatcgagg ccgtcctcgc tgttgtccgt
     1381 ctcgatgtcg agcccgtaga tcgttgcgag ttgtcccatc ggatccccac atgcccatgt
     1441 tctgcccctg cacggaaaat cgtaggaggg gcgcgcgtgc aggtggtgga tgcccccacg
     1501 ccccgcgcga acgatcgcgc gaggcgtgcc gatgcggagt tccatcgagg ccgagacgcg
     1561 aacgatcccg cccggacggc gggatcgaat caggtggaag cggcgaggcg gccggctccc
     1621 ggtgagcggt cgttcaccga gatcacctgg gccttgaagg cccggagagg cggaaccggc
     1681 agcgaacgag tgacgagacc cgagagggcg aggcccgacc tagcgctctt ctttgaagat
     1741 gacgtgctgg cggatcttgg ggtcgtactt catcagctcg atgcgctcgc gcgtgttggt
     1801 cttgttcttg cgcgtcacgt acgtgtagcc ggtgccggcc gtggacttca gcttgatgat
     1861 cgggcgcttg tcgctcttcg ccatggtgca ggtctttccc ggtgggggag ggtgcgggcc
     1921 agtcgacctg gcccgttgga cggacgagcc atcgtagggc cgatccgggt ccgtgcccaa
     1981 ctcggctcgg ggcccggtcg gccgatgggg ccctgggcga cccgcggcct agcctgcacg
     2041 atcgatgcgc ccgatccgct ccgtccggct cctccgtgcc ctggggatcc tcgcgttcgc
     2101 cctgctcgcc gctttgatcc cggcccgggc ggcttcggcc cacgccgagc tgatcgccac
     2161 cgatccggcc gacggtgcgg tggtcgacaa ggcgcccgag gcgctgacga tgcagttcag
     2221 cgagggcgtc tcggtccggc ccgacgggat ccgggtgctc gatgccacag gcgagcgggt
     2281 cgatgccggc gccgcctcgg cagac
//
LOCUS       DATMFS010000669         8150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_183728, whole genome shotgun sequence.
ACCESSION   DATMFS010000669 DATMFS010000000
VERSION     DATMFS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183728"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1383
                     /locus_tag="VNQ33_12775"
     CDS             79..1383
                     /locus_tag="VNQ33_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HWJ99029.1"
                     /translation="MPKTRDDHPPAHPHVHSHGSLRLRTGSPRVARLLAAGIGLCLLL
                     TVVGLVVLRPVGPVTKAPGVIPGTVFVSGTVNAVIPDVCPDQYDATIECLTAEVRVTS
                     GPENGELTDLPVTRGVPGAPDLRAGDHIRMSYFAKGPAGYKYQFDDYQRGRPVFLLAC
                     LFVAAVVALSRMQGIRALLGMAISLVVIVAFLLPALLRGGPPLPLALVTAIVVAFSAL
                     YLAHGIHVGTHVAFAGSVLSLLLTTLLGASFIGLAHFTGFSDENAVSLTVAAGRLDLR
                     GLILAGLVIGSLGVLDDMTVTQVSAVSELQAADPGRSALDLYRSGLRIGRDHVASTVN
                     TLVLAYAGASLPLLLMFSQGGRSVWGVVTTETVAVEAVGALVGSIGLIASVPITTGLA
                     AWVVHRADAPDGEADESDRPDDPSSGDARWESGDDRTFWERR"
     gene            complement(1429..2022)
                     /locus_tag="VNQ33_12780"
     CDS             complement(1429..2022)
                     /locus_tag="VNQ33_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HWJ99030.1"
                     /translation="MTAAERFPITFDDDANELLAKDPLALLYGMLLDQQVPMTWAFKS
                     PLVLKERFGDRWSPEGIASTSEEDVVEIFCRKPAVHRYPAAMARRAHAIATDIVEEYD
                     GDTASIWTTAKSGETLFRRVSSLPGFATEKSQIFVALLAKRFDVKPRGWKAAAGAFSD
                     DQPRSAADVDSLPKLREVQAWKKAQKAAKKSKSEFSL"
     gene            2108..3298
                     /locus_tag="VNQ33_12785"
     CDS             2108..3298
                     /locus_tag="VNQ33_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ99031.1"
                     /translation="MVSVHIAYTPEQEALRKELRAYFADLMTPEVIAAAAAGETGSPE
                     CLEAVRQMGRDRWLGVGWPEEYGGRGFGPVEQFIFMNESWRAGAPTPFLSINTVGMTI
                     MQFGTQEQKDFFLPKILAGELHFSIGYTEPNSGTDLASLTTKAVKDGDEWVINGQKTF
                     TSLANYADYIWLAARTDPDVPKHKGITMFLVPATDPGYSYTKIHTMVNASTFHTFYDD
                     VRVPDSAILGELHGGWGLIINQLNYERVGLAPPGMIERTYEDTLAWAREHTTPDGKRV
                     IDQEWVQLNLAKVRAGIEFLTLLNWKVAALEAAGEQVNPADASSIKVFGTEFFTDAYQ
                     LLAEIIGQAANLTADSPAAELASRINRGAQGSLILTFGGGVNEIQRDLIAMFGLGMPR
                     MPRM"
     gene            3311..4432
                     /locus_tag="VNQ33_12790"
     CDS             3311..4432
                     /locus_tag="VNQ33_12790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ99032.1"
                     /translation="MDFALSEEQTALRDLAGQILSEQLPADRIRDIEAGDDWFAADTW
                     SELAKADLLGLSLPEAHGGGGYGFYELALVLEQAGRNVAPLPLLSTLVLGALPIAEFG
                     TADQQAAHLPGVIDGSVLLTAALSEGGDGLPPAVPATTATASGAGWTISGTKDLVPGA
                     VHAARVLVPARTGDGTSTVFLVDPKAGGVSAEVNHGPNLEPLTTFTFDGVAVTAADVV
                     GTVDGGAGVVTWVADRAVAAYCAVTAGVCEAATRTTASYTSERKQFDTPIATFQAVAH
                     RAADAYIDTEAVRLTAYQAAWRLGEGLPAAEELAIAKYWAADGAHRVVHAAQHLHGGI
                     GVDTDYPVHRTFRWAKHLELSLGGGTAHLRRLGALLAAG"
     gene            4528..5928
                     /locus_tag="VNQ33_12795"
     CDS             4528..5928
                     /locus_tag="VNQ33_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ99033.1"
                     /translation="MKIRVVALCCAVALSAAACGSRLSDDELAAGGGTGGGSAAASST
                     GGTSSAGISEGEGGPKIGTLAVPCGKGDQAPAEPAEKVPGVSADTIKIAVISDKAGQV
                     KVPTASIEESMQAFTDWCNGFGGINGRKLELTKIDSKLFSHLEATKEACNAGVFAIVG
                     SGSVTDNQGAQQMVDCGLIEVPGYTATAAKGLSDNVVAPVPNPSNFYPIGGALWVKQQ
                     HPEAVKKAAILYSGIDTASVQAERIKLAYEAQGYDFVYDKETGVVQESYATEASEMKA
                     KGVQWVTMVSATSETAKLLRDMKTQDFQPEVVDLGQQYYDPDLLKEPGAEGAIVQLNT
                     VPFEEVDDSPALQAYMAAYDKVGAKVDPTSLGVQSFSAGLLFATAAKSVGDDITRASV
                     LAALKDIHKWDGGGLHFLADPGANKSSNCFMYMEVVDGAFKRLWPEKPTTFDCDDSYR
                     YDLKDDFGGGAKLKSS"
     gene            5928..8048
                     /locus_tag="VNQ33_12800"
     CDS             5928..8048
                     /locus_tag="VNQ33_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ99034.1"
                     /translation="MNELGPLLVFGLASAAIYAVAASGLVVTYQTSGIFNFAHGAVAM
                     WAAFVYWQLSSPDAWGLPVWLALVLTLGVFAPAFGLLIDKVLMRRLHDASPITRIVVP
                     IGLLVALIQLATVVWSPAESRNLPRFFDGDVVRIAGVGVDYHQIVVFVVAVVVAVALR
                     ILLYRTRVGVAMRAVVDSRELAGLNGVSPDRISSLSWAVGCTLAAVAGVLIAPILTLD
                     QTTLTLLVINAYAAAVVGRLRSLPLTVLGAVILGLLREAASRYSTDINAWMAPGTEPG
                     WVPDRAAGLLHTMAGWINVETVPVIMLFIALLVVPQEKASLFNRQPDRSRVPNPSMRS
                     VAIASAALVVAASIVPSLLDGTALDAVGYGLALAIVALSLVPLMGYGGQISLAPLAFA
                     GIGALVMYEWGSDGNPFALILVVLICGVVGALVALPSLRLKGLYLALSTMAFALFCEK
                     AVFAGVPVFSQGDASYERLHLGPVDTSSNRANLVLMAVVFALLGLLVTFIRRGPFGRR
                     LQAMKDSPAACATLGLDLTRLKLQVFALSAAIAGIGGALLGGWRGKVGTEQFALLQGA
                     LPGLPLVLMAVVGGIAAIAGVLLGAMLYSMFPLIGDTWPALKNLMTVLPGLAGISLAS
                     NPDGAIAQTADKVRAVWAKRSGASRHDPHRNRAMELLSPPPPEVVPELVLAGGGSLSS
                     DEREAIDAELGLSWGRCHADPRDA"
BASE COUNT         1142 a         2972 c         2781 g         1255 t
ORIGIN      
        1 cgttggtgca tgccgtactg tttcgcgcaa atgggaatga ttgtcaatct catttgaaag
       61 gtgtctaacg tctcccccgt gccgaagacc cgtgacgacc atccgcccgc gcacccgcac
      121 gtccactcgc acggcagctt gcggctccgc accggctcgc cgcgcgtggc gcgcctgctg
      181 gccgccggga tcggcctgtg cctgctgctc acggtggtcg gcctcgtggt gctccggccg
      241 gtgggcccgg tcacgaaggc cccgggcgtg atcccgggga ccgtgttcgt cagcggcacc
      301 gtgaacgcgg tgatccccga tgtctgcccg gaccagtacg acgcgacgat cgagtgcctg
      361 accgccgagg tgcgggtgac gagcggaccg gagaacggcg agctgaccga cctgcccgtc
      421 acgcggggcg tgcccggggc gcccgacctc cgcgccgggg accacatccg gatgtcgtac
      481 ttcgccaagg ggccggccgg gtacaagtac cagttcgacg actaccagcg gggccggccg
      541 gtcttcttgc tggcctgtct gttcgtggcg gcggtcgtgg cgctcagccg gatgcagggc
      601 atccgggccc tgctcgggat ggcgatcagc ctcgtggtga tcgtcgcctt cctccttccc
      661 gcgctcctgc gcgggggtcc accgcttccc ctggcgctcg tcaccgcgat cgtcgtggcg
      721 ttcagcgccc tctacctcgc ccacggcatc cacgtcggca cccatgtggc cttcgccggg
      781 tcggtgctgt cgctcctgtt gacgacgctc ctcggcgcgt cgttcatcgg cctggcccac
      841 ttcaccgggt tcagcgacga gaacgccgtg agcctcacgg tggccgccgg ccggctcgac
      901 ctccgggggc tgatcctggc cggtctcgtc atcgggtccc tcggcgtcct cgacgacatg
      961 acggtcacgc aggtctccgc ggtgtcggag ctgcaggccg cggacccggg ccgcagcgcc
     1021 ctggacctgt accggtcggg actccgcatc ggccgggacc acgtcgcatc gacggtgaac
     1081 accctcgtcc tcgcctacgc cggcgcatcg ctgccgctcc tcttgatgtt ctcccagggc
     1141 ggccgatccg tgtggggcgt cgtcaccacc gagaccgtcg ccgtcgaggc cgtcggcgcc
     1201 ctcgtcggct cgatcggcct catcgcctcg gtcccgatca ccaccggcct ggccgcctgg
     1261 gtcgtccacc gcgccgatgc acccgacggc gaagccgacg agtccgaccg gcccgacgac
     1321 ccttcatccg gcgacgcccg ctgggagtcc ggtgacgacc ggaccttctg ggagcgccgc
     1381 tgagcgctcc tcggccgcga cggtctccac tcgacccgag agccagggct acagggagaa
     1441 ctcggacttg gacttcttgg cggccttctg ggccttcttc caggcctgga cctcgcggag
     1501 cttggggagg gagtcgacgt cggcggcgga gcggggctgg tcgtcgctga aggcgccggc
     1561 ggcggccttc cagccgcggg gcttcacgtc gaagcgcttg gcgaggaggg ccacgaagat
     1621 ctggctcttc tcggtggcga acccgggcag gctcgacacc cgcctgaaga gggtctcgcc
     1681 cgacttggcg gtggtccaga tcgaggcggt gtcgccgtcg tactcctcca cgatgtcggt
     1741 ggcgatcgcg tgcgcccggc gagccatggc ggcggggtag cggtgcacgg ccggcttgcg
     1801 gcagaagatc tcgaccacgt cctcctcgga cgtcgacgcg atgccctcgg gtgaccagcg
     1861 gtcgccgaag cgctccttca agacgagggg cgacttgaac gcccaggtca tcggcacctg
     1921 ctggtccagc agcatcccgt agagcagcgc cagcgggtcc ttggccagca gctcgttggc
     1981 gtcgtcgtcg aacgtgatcg ggaagcgctc cgctgcagtc acggccgcga ggctagatcg
     2041 cccgcctggg aacggcgaga gggcaccgtg ctagaacgtg ttctagttct gatggaccgt
     2101 caggggaatg gtttccgtgc acatcgcgta cacgcctgag caagaagcgc tccgcaagga
     2161 gcttcgtgcc tacttcgccg atctgatgac cccggaggtc atcgccgctg ctgctgcggg
     2221 ggagaccggc agccccgagt gcctcgaggc cgtccggcag atgggccgcg accgctggct
     2281 cggcgtcggc tggccggagg agtacggggg ccgcggcttc gggcccgtcg agcagttcat
     2341 cttcatgaac gagtcctggc gggcgggcgc ccccacgccg ttcctgtcga tcaacaccgt
     2401 cggcatgacg atcatgcagt tcggcacgca ggagcagaag gacttcttcc tcccgaagat
     2461 cctcgccggc gagctgcact tctcgatcgg ctacacggag cccaactcgg gcaccgacct
     2521 cgcctcgctc accaccaagg cggtgaaaga cggcgacgag tgggtgatca acgggcagaa
     2581 gaccttcacc tcgctggcca actacgccga ctacatctgg ctcgccgccc gcaccgaccc
     2641 cgatgtcccc aagcacaagg gcatcacgat gttcctggtg cccgccaccg accccgggta
     2701 ctcgtacacg aagatccaca cgatggtgaa cgccagcacg ttccacacgt tctacgacga
     2761 cgtgcgggtg ccggactccg ccatcctcgg cgagctccac ggcggctggg gcctcatcat
     2821 caaccagctc aactacgagc gggtgggcct ggccccgccg gggatgatcg agcgcaccta
     2881 cgaggacacg ctcgcctggg ctcgggagca cacgaccccc gacggcaagc gggtgatcga
     2941 ccaggagtgg gtgcagctca acctggcgaa ggtgcgtgcc ggcatcgagt tcctcaccct
     3001 cctcaactgg aaggtggcgg cgctcgaggc agcgggggag caggtcaacc cggccgacgc
     3061 cagctccatc aaggtgttcg gcaccgagtt cttcaccgac gcctaccagc tgctggccga
     3121 gatcatcggc caggccgcca acctcacggc ggactccccg gcggccgagc tggcctcgcg
     3181 catcaaccgg ggcgcccagg gctccctgat cttgaccttc ggcggaggcg tgaacgagat
     3241 ccagcgcgac ctcatcgcca tgttcggcct gggcatgccc cgcatgccgc ggatgtagga
     3301 gacagacacc atggacttcg cactctccga agagcagacg gccctgcgcg acctcgccgg
     3361 gcagatcctg agcgagcagc tcccggccga tcgcatccgc gacatcgagg ccggcgacga
     3421 ctggttcgcc gccgacacct ggagcgagct ggccaaggcc gatctgctcg ggctgtcgct
     3481 ccccgaggcc cacggcggcg gtggctacgg cttctacgag ctggccctgg tcctggagca
     3541 ggcgggccgc aacgtggcgc cgctcccgct cctgtcgacc ctggtgctgg gcgccctgcc
     3601 catcgccgag ttcggcacgg ctgaccagca ggccgcgcac ctgcccgggg tgatcgacgg
     3661 cagcgtcttg ctcaccgcgg ccctgtccga aggcggcgac ggcctcccgc ccgccgtgcc
     3721 ggccaccacc gccacggcgt ccggcgccgg ctggaccatc agcggcacca aggacctggt
     3781 gcccggcgcc gtgcacgcgg ctcgcgtcct cgtgcccgcc cgcaccggcg acggcacctc
     3841 gacggtgttc ctcgtggacc cgaaggccgg gggcgtcagc gcggaggtca accacggccc
     3901 gaacctggag ccgctcacca cgttcacgtt cgacggcgtg gccgtcacgg ccgccgacgt
     3961 ggtcggcacg gtcgacggcg gggccggtgt ggtcacctgg gtcgccgacc gggccgtcgc
     4021 ggcgtactgc gccgtcaccg ccggcgtgtg cgaggcggcg acccggacca ccgccagcta
     4081 cacgagcgag cggaagcagt tcgacacgcc gatcgccacg ttccaggcgg tcgcccaccg
     4141 ggcggccgac gcctacatcg acaccgaggc cgtgcgcctc accgcctacc aggcggcctg
     4201 gcggctgggc gaggggctcc cggcggccga ggagctggcc atcgccaagt actgggcggc
     4261 cgacggcgcg caccgcgtcg tccacgccgc ccagcacctg cacggcggca tcggcgtcga
     4321 caccgactac ccggtgcacc gcacgttccg gtgggcgaag cacctggagc tcagcctcgg
     4381 gggcgggacc gcccacctgc ggcgcctcgg cgcgctgctc gccgccggct gatcggcccg
     4441 cggcgtcccc gaacctgacg gcccgtcagg tcggtgttca cccgcgcgct acattcctcc
     4501 accgagcatc tgtaagggga cctgcttgtg aagatccgtg tcgttgcgct gtgctgcgcc
     4561 gtcgccctct cggcggccgc gtgcggcagc cgactgtccg atgacgagct ggcggcgggc
     4621 ggtgggaccg gcggcggctc ggcggcggcg agcagcaccg gcggcaccag cagcgccggc
     4681 atctccgagg gcgagggcgg tcccaagatc ggcaccctcg cggtcccgtg cggcaagggc
     4741 gaccaagcgc ccgccgagcc ggccgagaag gtccccggcg tcagcgccga caccatcaag
     4801 atcgccgtca tctccgacaa ggccggccag gtcaaggtgc ccacggccag catcgaggag
     4861 tcgatgcagg cgttcaccga ctggtgcaac ggcttcggcg ggatcaacgg ccgcaagctc
     4921 gagctcacca agatcgactc gaagctcttc agccacctgg aggccaccaa ggaggcgtgc
     4981 aacgccgggg tgttcgccat cgtcggctcc ggctcggtga ccgacaacca gggcgcccag
     5041 cagatggtcg actgcggcct catcgaggtg ccgggctaca cggccacggc ggccaagggc
     5101 ctgagcgaca acgtggtcgc cccggtgccc aacccgtcga acttctaccc gatcggcggt
     5161 gccctctggg tcaagcagca gcaccccgag gcggtgaaga aggccgccat cttgtactcc
     5221 ggaatcgata cggcgtcggt ccaggccgag cgcatcaagc tcgcctacga ggcacagggc
     5281 tacgacttcg tctacgacaa ggagacgggc gtcgtgcagg agagctacgc caccgaggcc
     5341 tcggagatga aggccaaggg cgtgcagtgg gtgaccatgg tgtcggccac gtcggagacc
     5401 gccaagctcc tgcgcgacat gaagacccag gacttccagc cggaggtcgt cgacctcggc
     5461 cagcagtact acgacccgga cctgctcaag gagccggggg ccgagggcgc catcgtccag
     5521 ctcaacaccg tgccgttcga agaggtcgac gattcgccgg cgctccaggc ctacatggcc
     5581 gcctacgaca aggtcggcgc caaggtcgac cccacgtcgc tcggcgtgca gtcgttctcg
     5641 gccgggctgc tgttcgccac cgccgccaag tccgtcggcg acgacatcac ccgggccagt
     5701 gtcctcgccg cgctgaagga catccacaag tgggacggcg gcggcctcca cttcctggcc
     5761 gatcccgggg cgaacaagag ctccaactgc ttcatgtaca tggaggtcgt ggacggggcg
     5821 ttcaagcggc tctggcccga gaagcccacg acgttcgact gcgacgactc gtaccgctac
     5881 gacctgaagg acgacttcgg cggcggggcc aagctgaagt cctcgtagtg aacgagctcg
     5941 ggccgctcct ggtcttcggt ctggccagtg cggccatcta cgcggtcgcc gcgagcggcc
     6001 tcgtggtcac gtaccagacg tcgggcatct tcaacttcgc ccacggcgcc gtggccatgt
     6061 gggcggcgtt cgtctactgg cagctgagct cgcctgacgc gtggggcctg cccgtgtggc
     6121 tcgccctcgt gctgacgctg ggcgtgttcg ccccggcgtt cgggctcctc atcgacaagg
     6181 tgctgatgcg gcgcctccac gacgcctcgc ccatcacccg gatcgtcgtc ccgatcggcc
     6241 tgctggtggc gctgatccag ctggcgaccg tggtctggtc gccggccgag tcccgcaacc
     6301 tgccccgctt cttcgacggc gacgtcgtgc gcatcgccgg cgtcggcgtc gactaccacc
     6361 agatcgtggt cttcgtggtg gccgtcgtcg tcgccgtcgc gctgcgcatc ctcctgtacc
     6421 ggacccgcgt cggggtggcc atgcgggccg tggtcgacag ccgtgagctg gccggcctca
     6481 acggcgtctc gccggaccgg atctcgtcgc tgtcgtgggc cgtcggctgc acgctcgccg
     6541 ccgtggccgg cgtgctcatc gccccgatcc tgacgctgga ccagaccacg ctcacgctgc
     6601 tcgtgatcaa cgcgtacgcg gcggccgtgg tcggtcggct gcgcagcctc ccgctcaccg
     6661 tgctcggcgc cgtgatcctc gggctgctcc gggaggcggc gtcgcggtac tccaccgaca
     6721 tcaacgcgtg gatggcgccg gggaccgagc cgggctgggt gcccgaccgg gccgctgggc
     6781 tcctccacac gatggccggc tggatcaacg tcgagacggt gccggtgatc atgttgttca
     6841 tcgccctgct ggtcgtcccg caggagaagg cgtcgctctt caaccggcag cccgaccgca
     6901 gccgggtccc gaacccgtcg atgcgctcgg tggccatcgc ctccgcggcg ctggtcgtgg
     6961 ccgcgtccat cgtgccgtca ctgctcgacg gcaccgcgct cgacgccgtc ggctacggcc
     7021 tcgccctcgc catcgtggcc ctctccctcg tgccgctcat gggctacggc ggccagatct
     7081 cgctcgcccc gctggcgttc gccggcatcg gcgccctggt catgtacgag tgggggagcg
     7141 acggcaaccc gttcgccctc atcctcgtgg tgctcatctg cggcgtggtc ggggccctgg
     7201 tggccctgcc ctcgctgcgc ctcaaggggc tgtacctcgc actgtccacg atggcgttcg
     7261 ccctgttctg cgagaaggcc gtgttcgccg gtgtcccggt cttcagccag ggcgatgcgt
     7321 cctacgagcg cctgcacctc ggccctgtcg acaccagcag caaccgggcc aacctcgtgc
     7381 tcatggccgt cgtgttcgcg ctgctgggcc tgctcgtcac gttcatccgg cgggggccgt
     7441 tcggccgccg cctccaggcg atgaaggaca gcccggcggc gtgcgccacc ctcggcctcg
     7501 acctcacccg gctcaagctc caggtgttcg ccctctcggc ggccatcgcc ggcatcggcg
     7561 gcgccctgct gggcggctgg cgcggcaagg tcggcaccga gcagttcgcc ctgctgcagg
     7621 gcgccctgcc cggcctgccc ctcgtgctca tggccgtggt cggcggcatc gccgccatcg
     7681 ccggggtgct gctgggggcg atgctctact cgatgttccc gctgatcggc gacacgtggc
     7741 cggcgctgaa gaacctgatg acggtgctcc cgggcctcgc cggcatcagc ctcgccagca
     7801 accccgacgg tgccatcgcc cagaccgccg acaaggtgcg ggccgtgtgg gccaagcgct
     7861 ccggggcatc gcgccacgat cctcaccgca accgggcgat ggagctgctg tcgcccccgc
     7921 cccctgaggt ggtgcccgaa ctggtgctcg ccggcggcgg gtccctgtcg tcggacgagc
     7981 gcgaggcgat cgacgccgag ctcggcttga gctgggggag gtgccatgcc gatcctcgag
     8041 acgcgtgaga tcgccgtccg cttcggcggg cacctcgccg tgaacgacgt gagcctcacc
     8101 gtggacgccg gcgagatcac cgggctgatc gggcccaacg gcgccggcaa
//
LOCUS       DATMFS010000670         4085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_69527, whole genome shotgun sequence.
ACCESSION   DATMFS010000670 DATMFS010000000
VERSION     DATMFS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69527"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNQ33_12805"
     CDS             complement(<1..237)
                     /locus_tag="VNQ33_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99035.1"
                     /translation="MARPIDVDDVLRPKPEWPTLLWRGFAKHCPRCGGGHLYRNWFRM
                     KERCPTCGYLFEREPGFFVGAYFINFGIVEGFLFV"
     gene            590..895
                     /locus_tag="VNQ33_12810"
     CDS             590..895
                     /locus_tag="VNQ33_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99036.1"
                     /translation="MLMQIDGTLSVKAYGGPTGTQDEIQQIDIDDAGGSFTIDLDNFG
                     LGTTAAIPADATPTEVQAALEEVDGVPVGDRTRWRPGQRRRWRLPLTWALTRLAGEI"
     gene            complement(877..950)
                     /locus_tag="VNQ33_12815"
     tRNA            complement(877..950)
                     /locus_tag="VNQ33_12815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Pro,seq:tgg)
     gene            1024..1764
                     /locus_tag="VNQ33_12820"
     CDS             1024..1764
                     /locus_tag="VNQ33_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ99037.1"
                     /translation="MDAAAERPRNPGLLELRVADDPEAWTAAGFTVDGDEVRIDPIAV
                     RLTGRPDDGPRGITGWTVAGMAIADGSLDGLRTDDRSEAERAAPTNSSPSFPPEPEPG
                     SPVHPNGTVGLDHVVVLTPDLDRTIAACEAAGLDLRRVRETTASGSPVRQAFFKLGPV
                     VLEVVSGDVGAGLPAADAPATFWGLAIDVADLDETAASMGEGLGRIKTAVQRGRRIAT
                     IRTQALDISTAIAAMDHHADRTTPDDGR"
     gene            1777..2382
                     /locus_tag="VNQ33_12825"
     CDS             1777..2382
                     /locus_tag="VNQ33_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99038.1"
                     /translation="MLDHVFTDAIGALRDTVEGAMLERQAFEERFQADVLLGDLTWET
                     SYALPGEGTPPRTRADLTLDWPTWSQTAYRSWYIGDAVDEPPRIEIEIVLRIQRLAEP
                     PDPAKVLDVLPAESSLIGSERLIRSGPTVETIHGEDLEEVEWAIEVSYEGTYELDEDV
                     LADGSKLDEHFSSMGGWIASTLVRLGDLTFEFAPPDELPAD"
     gene            2411..3196
                     /locus_tag="VNQ33_12830"
     CDS             2411..3196
                     /locus_tag="VNQ33_12830"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWJ99039.1"
                     /translation="MSELVITEVADQIATITLNRPEARNALSGALIRDLRTAVRDAQA
                     DDAVRVIILTGADPAFCAGLDLKELGGGSDTLTGTAPKDDTPIDQRGPLPPGPKPVIG
                     AVNGAAITGGFELALACDFLIASDRARFADTHARVGIQPWWGLTVLLPQAIGLRRARE
                     MSATGNFCDAQKALEWGLVNHVVPHDHLLPFARGIAADIVSSDQRAVQQIFATYAAGS
                     RVTVGEAWLVESDVAAEWQGQGLDPDEIERRRQGVTDRGRTQL"
     gene            complement(3203..3823)
                     /locus_tag="VNQ33_12835"
     CDS             complement(3203..3823)
                     /locus_tag="VNQ33_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99040.1"
                     /translation="MPTTPETPRPSWWHRRWPSALVFAAVLGAMAIASLVIWSDVSDV
                     LPDVGKDKQGQDPWNDPVGIERVGVFQVVHIPDCAAAPVVRIGLWDEDSKPYWEVSGP
                     PTPMDSFAIGAVPEGFAEDTAYEKPPAGAVLRLVVFRKVKGVAGVRYQETDLRTGYVV
                     SGTPLNRYEVDKFQTGSVCGDEDGEGDGATTTPVDDEAASTTVPAG"
     gene            complement(3897..>4085)
                     /locus_tag="VNQ33_12840"
     CDS             complement(3897..>4085)
                     /locus_tag="VNQ33_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99041.1"
                     /translation="DTDSLVTFCHAVGLGFLLLEVLDAPLPDNDAWEHLIGRLLAAVG
                     DPAAFARTAAADFAPVVE"
BASE COUNT          635 a         1518 c         1405 g          527 t
ORIGIN      
        1 cacgaagagg aagccttcga cgatgccgaa gttgatgaag taggcgccga cgaagaaccc
       61 gggttcccgc tcgaagaggt agccgcacgt cgggcaccgc tccttcatgc ggaaccagtt
      121 gcggtacagg tgcccgccgc cgcagcgcgg gcagtgcttg gcgaagcccc gccacaacag
      181 ggtcggccac tccggcttgg gccggaggac gtcgtcgaca tcgatcggac gggccatcgc
      241 ctcccatcat cgcccacccc gcggccgatc cccaccggcc cacggccgaa cgaccgggag
      301 gcaaggcacc gggcacgggc ggggcgcgag gcttcgcccc ggcgacgaaa ggcggtcgcc
      361 acgtgcaaac gagcccgtgg gcacccgccg gtgaagcgct ccgatccgag accatcctgc
      421 cgacacgctg gggggggtcg caggaggttc agcgtcggcg gtcaccaggg gaggcggtcg
      481 ccctgggagg gagaaccgat ggcgaagacg tggactcgca cagctgccgt ggcggcggtg
      541 gcaacagcgc tcgtgaccgg gctcttcgtg ggtctacacc cccgccttcg tgctcatgca
      601 gatcgacggc accctgagcg tcaaggccta cggagggccg accggcaccc aagacgagat
      661 ccagcagatc gacatcgacg atgccggcgg ctcgttcacg atcgacctgg acaacttcgg
      721 gctcgggacg accgctgcca tcccggccga tgcgactcct accgaggtgc aagcggccct
      781 cgaggaggtc gatggtgtgc cggtcggtga tcgaacgcgg tggcgcccag gtcagcggcg
      841 ccgctggaga ttgccgctga cctgggcttt gacccgtctg gccggtgaga tttgaactca
      901 cggcccccgc gtcccaaacg cggtgcgcta ccaaactgcg ccacggccag tccgggtcac
      961 ggtaccgggt gccgggcgag ccgccgaact cggttccccg gcccgccggt agggtccgcg
     1021 gccatggacg cagcggccga acgccctcgc aacccggggc tcctcgaact gcgggtcgct
     1081 gacgatcccg aggcgtggac ggccgccggg ttcaccgtcg acggcgacga ggtgcgcatc
     1141 gacccgatcg ccgtgcggtt gaccggccgc cccgacgacg gccctcgggg catcaccggg
     1201 tggacggtcg ccgggatggc catcgccgac gggtcgctcg acgggttgcg gaccgatgac
     1261 cgctccgaag cggaacgagc agcccccacg aactcctcac cgtccttccc gccggagccg
     1321 gagccgggtt cgccggttca tcccaacgga acggtcggac tcgaccacgt cgtcgtgctc
     1381 acgccggacc tcgaccggac gatcgcggcg tgcgaggcgg ccggcctgga cctgcgccgg
     1441 gtgcgcgaga ccaccgccag cggttcgccg gtgcgccagg cgttcttcaa gctcgggccg
     1501 gtggtgctcg aggtggtcag cggcgacgtc ggcgccggcc tccccgcggc cgacgcgccg
     1561 gcgaccttct gggggctggc catcgacgtc gccgacctcg acgagaccgc ggcgtcgatg
     1621 ggcgaggggc tcggccgcat caagaccgcc gtgcagcgcg gccggcgcat cgccacgatc
     1681 cgcacccagg cgctcgacat ctccacggcc atcgcggcga tggaccacca cgccgatcgc
     1741 accacccccg acgacggccg gtagggtccg gcgcagatgc tcgatcacgt gttcaccgac
     1801 gccatcggcg ccctccgaga caccgtcgaa ggggccatgc tcgaacgcca ggcgttcgag
     1861 gagcggttcc aggccgatgt cctcctcggg gacctgacct gggagacgtc ctacgcgctg
     1921 cccggcgagg gcaccccgcc gcggacccgt gccgacctca ccctcgactg gccgacctgg
     1981 tcccagacgg cctaccgctc ctggtacatc ggcgatgccg tcgacgagcc cccgcgcatc
     2041 gagatcgaga tcgtcctgcg catccagcgc ctggcggaac cgccggatcc ggccaaggtc
     2101 ctcgacgtgc tcccggccga atccagcctc atcggctccg agcggctcat ccgctccggg
     2161 ccgaccgtcg agacgatcca cggcgaggac ctcgaggagg tcgagtgggc gatcgaggtg
     2221 tcctacgagg gcacctacga gctcgacgag gacgtgctgg ccgacggctc gaagctcgac
     2281 gagcacttca gctcgatggg cggctggatc gcctccacgc tcgtgcggct cggcgacttg
     2341 accttcgagt tcgccccgcc cgacgaactg cctgcggact gaccgaaccc acgaccctcc
     2401 aggagcgaac gtgagcgagc tcgtcatcac cgaggtggcc gaccagatcg ccaccatcac
     2461 cctcaaccgg cccgaggcgc gcaacgccct ctcgggtgcg ctgatcaggg acctccgcac
     2521 ggcggtccgg gacgcgcagg ccgacgacgc cgtgcgcgtg atcatcctca cgggcgccga
     2581 ccccgccttc tgcgccggcc tcgacctcaa ggagctcggc ggcgggtccg acacgctcac
     2641 cggcaccgcc ccgaaggacg acacgcccat cgaccagcgc ggtcccctcc ctcctggccc
     2701 gaagccggtg atcggcgccg tcaacggggc ggccatcacc gggggcttcg agctggccct
     2761 cgcgtgcgac ttcctgatcg cctccgaccg ggcgcgcttc gccgacaccc acgcccgagt
     2821 gggcatccag ccgtggtggg gcctgacggt gctgctcccg caggcgatcg ggctgcgccg
     2881 cgcccgtgag atgagcgcca ccggcaactt ctgcgatgcc cagaaggccc tcgagtgggg
     2941 cctcgtcaac cacgtcgtgc cccacgacca cctgttgccg ttcgcccggg gcatcgccgc
     3001 cgacatcgtc tcgagcgacc agcgggccgt ccagcagatc ttcgcaacct acgcggccgg
     3061 atcccgggtg accgtcggcg aggcctggct cgtcgagtcc gacgtcgcgg ccgagtggca
     3121 gggccagggc ctcgaccccg acgagatcga gcggcggcgc cagggcgtca ccgaccgggg
     3181 ccgcacccag ctctgaagcc ggtcagccgg ccgggaccgt ggtggacgcg gcttcgtcgt
     3241 cgacgggggt ggtggtcgcg ccgtccccct cgccatcctc gtcaccgcag accgaaccgg
     3301 tctggaactt gtcgacctcg tagcggttca acggcgtgcc actcacgacg tagccggtcc
     3361 gcaggtcggt ctcctggtag cggacgccgg cgacgccctt caccttgcgg aagacgacga
     3421 gccgcagcac cgcaccggcc ggtggcttct cgtacgccgt gtcttcggcg aagccctcgg
     3481 gcacggcccc gatcgcaaac gagtccatcg gcgtcggcgg ccccgacacc tcccagtacg
     3541 gcttgctgtc ctcgtcccag aggccgatgc gcaccaccgg ggcagcagcg cagtcgggga
     3601 tgtgcacgac ctggaacaca ccgacccgct cgatccccac ggggtcgttc cacggatcct
     3661 ggccctgctt gtccttgccg acgtcgggga ggacgtcgct cacgtcggac cagatcacga
     3721 ggctcgcgat cgccatcgcg ccgaggacag cagcgaagac cagcgccgag ggccaccggc
     3781 ggtgccacca cgacgggcgc ggcgtctcag gcgtcgtggg catccgacca cgatcccaca
     3841 tctgccacgg ccggcagcaa ccacctcgga caggcggacg ctcccggccg caggagctac
     3901 tcgacgacgg gagcgaagtc cgccgccgcg gtgcgggcga acgccgccgg gtcgccgacg
     3961 gcggcgagca ggcggccgat caggtgctcc caggcgtcgt tgtcggggag cggcgcgtcg
     4021 agcacctcga gcagcaagaa gccgaggccc acggcgtggc agaacgtgac cagcgagtcg
     4081 gtgtc
//
LOCUS       DATMFS010000671         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_69578, whole genome shotgun sequence.
ACCESSION   DATMFS010000671 DATMFS010000000
VERSION     DATMFS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69578"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VNQ33_12845"
     CDS             complement(<1..196)
                     /locus_tag="VNQ33_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99042.1"
                     /translation="MTTAVLARDGSETTRPPPSRVHCGGFEILRAIAALMVVVHHAGS
                     YAVPPGTGGLYRLTEVMDSGV"
     gene            301..1950
                     /locus_tag="VNQ33_12850"
     CDS             301..1950
                     /locus_tag="VNQ33_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ99043.1"
                     /translation="MSDAAADTPVTTDQEEVVVIGAGPAGLTAAFQFHKHGVASTIIE
                     ADDMVGGISRTAQRDGWRFDIGGHRFFTKVQPVEDLWHEILPDEDFLMRPRKSRIFYN
                     GKYFDYPLKATNALKNLGIIEAVLCVFSYAWARVRPPKDQTNYEGWLVARFGWRLYRT
                     FFKTYTEKLWGVPVSEMPADWAAQRVKGLSLGNAIVNALLPKRNQKDITSLIEEFQYP
                     KHGPGMMWETCTEKVVAQGSTIHLETSVTKIAHAGGKAVTVTATAKDGTTTEHPATQV
                     ISSMPISQLLEAMDPPVPAEVKAAAEDLRYRDYMIVALAMPEELIGFDDNWIYIHDPK
                     VRTMRIQNFGSWSPYMVKEGRNTLGLEYTVWEGDDEWNADDDWLIERAKAELEQLGLC
                     KAGQIEAGWVVRQAKAYPIYDERYRANVDILRAWLGEHAGNVHPVGRNGMFRYNNQDH
                     SMFTAMLTVENIATGTTHDVWEVNVEEEYHEEATSSVKDKFIAEKEGRVPGSHGTGRD
                     APVIKREVIDAHKPPTTPAKGDDLAAMDHDAGTEIAGEPEA"
     gene            2046..2270
                     /locus_tag="VNQ33_12855"
     CDS             2046..2270
                     /locus_tag="VNQ33_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99044.1"
                     /translation="MTGFSESSENRRRRYGDDVTIDLFFGMLRRIPTAAELTEWAPLV
                     AADLQSKDGTGVEALAQHLFTSQEYLARVS"
BASE COUNT          427 a          836 c          708 g          310 t
ORIGIN      
        1 cgacgccgct gtccatgacc tccgtgaggc ggtagagccc cccggtcccg gggggaacgg
       61 catacgagcc tgcgtggtgg acgaccacca tcagcgcagc gatcgcccgc aggatctcga
      121 acccgccgca atgcacccgt gacggaggcg gacgagtagt ttcggagcca tcgcgtgcca
      181 gcacggccgt ggtcatcgag ctcccacccg ggcaacggcg gggggcgaac cggtgcccgt
      241 ggtcggtgac gtcacgccgc cgattcttcc acgcccaccc ctcaggacgg ttcttcctcc
      301 atgtccgacg ctgctgccga cacccccgtc accaccgatc aggaagaggt cgtcgtcatc
      361 ggggcgggac ctgccggact caccgctgcc ttccagttcc acaagcacgg tgtcgcctcg
      421 accatcatcg aagccgacga catggtgggc gggatctccc gcaccgcgca gcgcgacgga
      481 tggcgcttcg acatcggcgg gcaccgcttc ttcaccaagg tccagccggt cgaggacctg
      541 tggcacgaga tcctcccgga cgaggacttc ctcatgcggc cccgcaagag ccgcatcttc
      601 tacaacggca agtacttcga ctacccgctc aaggccacca acgccctcaa gaacctcggc
      661 atcatcgaag ccgtcctctg cgtcttctcc tacgcctggg cgcgcgtccg cccgccgaag
      721 gaccagacca actacgaggg ctggctggtc gcccgcttcg gctggcgcct ctaccgcacg
      781 ttcttcaaga cctacaccga gaagctctgg ggcgtgccgg tcagcgagat gccggccgac
      841 tgggcggcgc agcgggtgaa ggggctctcc ctcggcaacg ccatcgtgaa cgccctgctc
      901 cccaagcgga accagaagga catcacctcg ctcatcgagg agttccagta cccgaagcac
      961 ggccccggga tgatgtggga gacgtgtacc gagaaggtgg tggcccaggg ctccacgatc
     1021 cacctggaga cctcggtcac gaagatcgcc cacgccggcg gcaaggccgt caccgtcacc
     1081 gccacggcca aagacggcac caccacggag caccccgcca cccaggtcat ctcctcgatg
     1141 cccatctccc agctgctcga ggccatggac ccgccggtgc ccgccgaggt caaggcggcg
     1201 gccgaggacc tgcgctaccg cgactacatg atcgtggccc tggcgatgcc cgaggagctc
     1261 atcggcttcg acgacaactg gatctacatc cacgacccga aggtgcgcac catgcgcatc
     1321 cagaacttcg gctcctggtc gccgtacatg gtcaaggagg gccgcaacac cctcggcctc
     1381 gagtacacgg tctgggaggg cgacgacgag tggaacgccg acgatgactg gctcatcgag
     1441 cgggccaagg ccgagctcga gcagctcggc ctgtgcaagg ccggtcagat cgaggcgggc
     1501 tgggtggtcc gccaggcgaa ggcgtacccg atctacgacg agcgctaccg cgccaacgtc
     1561 gacatcctcc gggcctggct cggcgagcac gccggcaacg tccaccccgt gggccgcaac
     1621 ggcatgttcc ggtacaacaa ccaggaccac tccatgttca cggccatgct cacggtcgag
     1681 aacatcgcca ccggcaccac ccacgacgtg tgggaggtca acgtggagga ggagtaccac
     1741 gaggaagcca cctcgtcggt gaaggacaag ttcatcgccg agaaggaggg ccgggtcccc
     1801 ggttcccacg gcaccggccg cgacgccccc gtgatcaagc gcgaggtcat cgacgcccac
     1861 aagccgccca ccacccccgc caagggcgac gacctggcgg ccatggacca cgatgccggc
     1921 accgagatcg ccggcgaacc ggaggcctag accatgacgg cggcgacccc cacccctgct
     1981 tcggcgcggc cggcatcagc ttctggacca agcagctcga ccgcaagaag agccgcggcc
     2041 aggtcatgac cgggttctcc gagtccagcg agaaccggcg ccgccgctac ggcgacgacg
     2101 tgacgatcga tctgttcttc ggcatgctcc gccgcatccc caccgccgcc gagctcaccg
     2161 agtgggcgcc gctggtggcc gccgatctcc agagcaagga cggcaccgga gtcgaagccc
     2221 ttgcccagca cctgttcacg agccaggagt acctcgcccg ggtctcttga ccgactggtc
     2281 a
//
LOCUS       DATMFS010000672         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_277576, whole genome shotgun sequence.
ACCESSION   DATMFS010000672 DATMFS010000000
VERSION     DATMFS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277576"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VNQ33_12860"
     CDS             complement(<1..336)
                     /locus_tag="VNQ33_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWJ99045.1"
                     /translation="MDSWLIGLFEAAQASDGDVGGAALVAVGGYGRGDLAPGSDLDLL
                     LLYRGKNPPAELAEKIWYPIWDEGLKLGHSVRTVKEALDLSAEDLDTATSLLTIRHLA
                     GESGLTAEMG"
     gene            694..1878
                     /locus_tag="VNQ33_12865"
     CDS             694..1878
                     /locus_tag="VNQ33_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWJ99046.1"
                     /translation="MISAALVLFMTPGLAFFYGGMDRSRNVLNMLMMNFWCLLVLPVL
                     WVAVGFTLAQSPVENKFIGSFDNAFLKGLDISDGGMTLAVVAFLGMFAAITPALISGA
                     VADRMKFSAWAIFAPVWMLIVFVPVFKWVYGGWLGQRGSLDFAGGTAIHVNAGIAALA
                     AVLVLGKRKGWPKEGHPPHSMPLVMIGTGILWFGWFGFNAGSALEANGTAVQAFMNTF
                     LAAAAAGLAWCLVERVRDGHFTNLGAASGIVAGLVAITPAAGFVAGMSPIFIGLIAGV
                     ICCFAVGLKTKAGYDDALDVVGVHFVGGLVGSLLIGLFADPDFFGGDFMAGLFYGGGG
                     KLLLEQTIANASAIAYSFPVTLGLMYLLKVTIGVRVSEEIEVGGLDQAEHAETAYHEA
                     LV"
     gene            1908..2246
                     /locus_tag="VNQ33_12870"
     CDS             1908..2246
                     /locus_tag="VNQ33_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWJ99047.1"
                     /translation="MKLITAIIKPFKVDEVKTALKAAGVLGITVTEVNGFGRQGGHTE
                     TYRGTEYQIDFVPKVKLEVVADDPAVGEVIEAISTAARTGQIGDGKIWVTSVDELVRI
                     RTGEQGADAV"
     gene            complement(2321..>2725)
                     /locus_tag="VNQ33_12875"
     CDS             complement(2321..>2725)
                     /locus_tag="VNQ33_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HWJ99048.1"
                     /translation="LNVGGGYATFPGVLGTAITQVCEVEIARTGLSQDQADAAGIEYE
                     IGVVEATTRAGYYPGAEPLHAKVLAEKGSGRLIGGQLVGADRAGKRIDTLAAAITARM
                     TASSFVDLDLAYAPAVSPMWDAFQLAARSTLG"
BASE COUNT          413 a          947 c          901 g          464 t
ORIGIN      
        1 acccatctcg gccgtgaggc cgctctcgcc ggcgaggtgc cggatggtca ggagcgacgt
       61 cgccgtgtcg aggtcctcgg cggagagatc gagcgcttct ttgacggtcc gcacggaatg
      121 gccgagcttc agcccctcgt cccagatggg gtaccagatc ttctcggcga gctcggccgg
      181 cgggttcttg ccccggtaca gcaggagcag gtcgaggtcg gatcccggag cgaggtcgcc
      241 gcggccgtag ccgccgaccg cgacgagcgc agcaccgcct acgtcgccgt cgctcgcctg
      301 ggccgcctcg aagagaccga tcaaccagga gtccaccgtg gcccgccagg ccgcggagaa
      361 ctgggccccg cggagcgaac gatcgcgccg gagggaatcg agtctcgagg gtgcgccttc
      421 gcccggcatg gcacggagtg taggagcggt gggagcaccc acctggcgcc gcgaacgcct
      481 ctcttcatca tgacatccgc atggcacaaa tctttcgtaa tgattctctc tacgagggga
      541 aacactcacg aaacaccggc tccgtacgtt gcgccgcaag cggtccacag ccgtggcctg
      601 caaccagcaa cggaaggtga tcgcgccatg agcgtggaag aacgattggc agaactcgag
      661 gcgagtgtcg gtagcggcca catcgcctgg gtcttgatct cagcagcact cgtcctgttc
      721 atgacgcccg gtctggcgtt cttctacggc ggcatggacc gcagtcggaa cgtcctgaac
      781 atgctcatga tgaacttctg gtgcctcttg gtgctcccgg ttctctgggt cgccgtcggc
      841 ttcacgctgg cgcagtcccc cgtcgagaac aagttcatcg gctcgttcga caacgccttc
      901 ttgaagggcc tcgacatctc cgatggcggc atgacgctcg cggtggtcgc gttcctcggc
      961 atgttcgccg ccatcacccc ggcgctcatc tccggcgccg tcgccgatcg catgaagttc
     1021 tccgcctggg cgatcttcgc tccggtgtgg atgctcatcg tgttcgtgcc cgtcttcaag
     1081 tgggtctacg gcggctggct cggccagcgg ggttcgctcg acttcgccgg cggcaccgcc
     1141 atccacgtca acgccggtat cgcggccctc gccgccgtct tggtcctcgg caagcgcaag
     1201 ggctggccga aggaggggca cccgccccac tccatgccgc tggtgatgat cggcaccggc
     1261 atcctctggt tcggctggtt cggcttcaac gccggttcgg cgctcgaggc caacggcacc
     1321 gccgtccagg cgttcatgaa caccttcctc gccgccgccg ccgctggttt ggcctggtgc
     1381 ctggtcgagc gggtgcgcga cggccacttc accaacctcg gtgcggcgtc gggcatcgtc
     1441 gccggcctgg tggccatcac ccccgccgcc ggcttcgtcg ccgggatgtc gccgatcttc
     1501 atcggtctca tcgccggtgt catctgctgc ttcgctgtcg ggctcaagac caaggcgggc
     1561 tacgacgacg ccctcgacgt cgtcggcgtc cacttcgtcg gcgggctcgt gggctccttg
     1621 ctcatcggct tgttcgctga tcccgacttc ttcggcggcg acttcatggc cggcctcttc
     1681 tacggtggcg gcggcaagct gctcctcgag cagaccatcg ccaacgcctc ggccatcgcc
     1741 tactccttcc cggtcaccct cggcctcatg tacctgctga aggtcaccat cggcgtgcgg
     1801 gtctccgagg agatcgaggt gggtggcctc gaccaggccg aacatgccga gaccgcttac
     1861 cacgaggccc tcgtctgacc ctcgggcacc taccgaaagg aactgcaatg aagctcatca
     1921 cagcaatcat caagcccttc aaggtcgacg aggtgaagac ggcgctcaag gcggccgggg
     1981 tcttggggat caccgtcacc gaggtcaacg gcttcggacg ccagggcggc cacaccgaga
     2041 cctaccgcgg caccgagtac cagatcgact tcgtcccgaa ggtcaagctg gaggtcgtgg
     2101 ccgacgatcc cgccgtcggc gaggtgatcg aagcgatctc caccgccgct cggaccggcc
     2161 agatcggcga cgggaagatc tgggtgacca gcgtcgacga gctggtccgg atccggaccg
     2221 gcgagcaggg cgccgacgcc gtctgacggc cggcaccacc gaacaccggg caccgcctcc
     2281 gatccaccgg atcggaggcg gtgccgcgtc cgcacccgag tcagccgagg gtggagcggg
     2341 ccgcgagctg gaacgcgtcc cacatcggcg agaccgccgg cgcgtaggcg aggtccaggt
     2401 cgacgaacga cgaggcggtc atcctggcgg tgatcgcggc ggcgagggtg tcgatgcgct
     2461 tccccgcccg atcggcgccg accagctgac cgccgatcag gcggcccgat cccttctcgg
     2521 ccagcacctt ggcgtggagc ggctcggctc cggggtagta cccggcacgg gtggtggctt
     2581 cgaccacgcc gatctcgtac tcgatgccgg cggcgtcggc ctggtcctgg ctgagcccgg
     2641 tgcgggcgat ctcgacctcg cacacctggg tgatcgccgt gcccagcacg ccggggaagg
     2701 tggcgtaccc gccgccgacg ttgag
//
LOCUS       DATMFS010000673         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_254542, whole genome shotgun sequence.
ACCESSION   DATMFS010000673 DATMFS010000000
VERSION     DATMFS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254542"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VNQ33_12880"
     CDS             <1..753
                     /locus_tag="VNQ33_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWJ99049.1"
                     /translation="RAATETAAAEAEETADERDRSDELLRAANAAVAQAATEQHAAQG
                     RVEDALADRRTARLKSTVEAIDIPLVALHAYWRASQLAPCRIPWWVIAGIGRVESGHG
                     SAHGSSLTAEGDTTVHILGIPLDGRPGVAAIGDTDGGRLDGDARSDRAVGPMQFLPGT
                     WNRWASDRNADGEADPHNIYDAAGAAARYLCFSQGDLDDEGKIRGALLAYNRSVPYGT
                     KVLAEGGSYRDALELPDVPESRPDDIPPDRGN"
     gene            complement(780..1295)
                     /locus_tag="VNQ33_12885"
     CDS             complement(780..1295)
                     /locus_tag="VNQ33_12885"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWJ99050.1"
                     /translation="MAASAPTASTTDRFTRFSTPVDDLILTGDGTSITGCYFATHRHH
                     DDPSAGLERDDGAFTGAVAQLGEYFDGERTAFDLPLAPTGTEFQLRVWAQLRTIPYGA
                     TCSYADIARGIGASNGFRAVGLANGRNPISIIVPCHRVIGADGSLTGYGGGLERKRFL
                     LDLESGALPFR"
     gene            1418..>2359
                     /locus_tag="VNQ33_12890"
     CDS             1418..>2359
                     /locus_tag="VNQ33_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ99051.1"
                     /translation="MIEARGLTKRYGPTTAVDDLSFRVESGRVTGFLGPNGAGKSTTM
                     RMCLDLDRADAGAVRIDGKRLIDFPKPMRSVGALLDAGYLHPTRKAKEHLWALAASNG
                     LPKKRVDHVLGLVGLREVANKRVGSFSLGMKQRLGLAASMLGDPQTLVFDEPANGLDP
                     EGILWIRNFLKGLASEGRTVFVSSHLLSEMALIADDLVVIGKGALIFQGPVDAFVDSA
                     AKKWVTVRSPQLQQIGDALHNGGAQVVAAGPGAIDVVGPTAAQIGELAASLGATLHEL
                     SPRTASLEDVFLEVTAGAQEYRGDTGAVGASAPPPPPP"
BASE COUNT          354 a          892 c          832 g          281 t
ORIGIN      
        1 cgcgccgcca ccgagacggc ggctgcggag gccgaggaga cggccgacga gcgcgaccgc
       61 agcgacgagc tcctgcgggc ggccaacgcc gccgtcgccc aggccgccac cgagcagcac
      121 gccgcccagg gccgggtcga ggacgccttg gccgaccgcc ggactgctcg gctgaagtcg
      181 acggtcgagg cgatcgacat ccccctggtc gccctgcacg cctactggcg ggcctcgcag
      241 ctggcgccgt gccggatccc ctggtgggtg atcgccggca tcgggcgcgt cgagtccggc
      301 cacgggtccg cacacggcag ctcgctcacc gccgaaggcg acaccacggt ccacatcctc
      361 ggcatcccgc tcgacggccg gcccggcgtg gcggccatcg gcgacaccga cggcggacgc
      421 ctcgacggcg acgcccgctc cgaccgcgcc gtcggcccga tgcagttcct tccgggcacc
      481 tggaaccggt gggccagcga ccgcaacgcc gacggcgaag cggacccgca caacatctac
      541 gacgccgccg gcgccgccgc ccgctacctc tgcttcagcc aaggcgacct cgacgacgag
      601 ggcaagatcc gcggcgccct gctcgcctac aaccgctcgg tcccctacgg cacgaaggtg
      661 ctggccgagg gcgggagcta ccgcgatgcg ctggagctgc ccgacgtccc ggagtcccga
      721 cccgacgaca tcccacccga ccgcggcaac tgacccgcct cggaccgcct cggaccgcct
      781 cagcggaagg gcagcgcccc ggactccagg tcgaggagga accgcttgcg ctcgagaccg
      841 ccgccgtagc cggtgaggct gccgtccgcc ccgatcaccc ggtggcaggg gacgatgatc
      901 gagatcgggt tgcgcccgtt ggccagcccg accgcccgga aaccgttgga ggcgccgatg
      961 ccgcgggcga tgtcggcgta gctgcacgtc gccccgtacg ggatggtccg cagctgcgcc
     1021 cacacccgga gctggaactc ggtgccggtc ggggcgagcg gaaggtcgaa ggcggtgcgc
     1081 tcgccgtcga agtactcgcc gagctgggcg acggccccgg tgaacgcgcc gtcgtctcgc
     1141 tccagccccg ccgacgggtc gtcgtggtgg cggtgggtcg cgaagtagca gccggtgatc
     1201 gaggtgccgt cacccgtgag gatcaggtcg tcgaccgggg tggagaaccg ggtgaaccga
     1261 tcggtcgtgg aagcggtggg ggcggaagcg gccatgcgcg gcacggtacg cctcggcccg
     1321 gtccaccact agcgggaatc agacgcccgg ggcggcgggg ccggagccgg ccggtacggt
     1381 tgccggaccg acccacgatg gcgtccagga gcggcgaatg atcgaagcac gagggctgac
     1441 gaagcgctac ggaccgacca ccgcggtcga cgacctgagc ttccgcgtcg agagcgggcg
     1501 ggtcacgggc ttcttgggcc ccaacggcgc cggcaagtcc acgaccatgc ggatgtgcct
     1561 cgacctcgac cgagccgacg ccggcgcggt ccgcatcgac ggcaagcgcc tcatcgactt
     1621 ccccaagccg atgcgcagcg tcggcgccct cctcgacgcc ggctacctgc acccgacccg
     1681 caaggcgaag gagcacctct gggccctcgc cgcgtccaac ggcctcccca agaagcgggt
     1741 cgaccacgtc ctcggcctgg tcggtctccg tgaggtggcg aacaagcgcg tcggctcgtt
     1801 ctcgctgggc atgaagcagc ggctcggcct cgccgcgtcc atgctcggcg acccgcagac
     1861 cctggtgttc gacgagccgg ccaacggcct ggaccccgag ggcatcttgt ggatccgcaa
     1921 cttcctcaag ggcctggcga gcgagggccg gacggtcttc gtctcgagcc acctgctctc
     1981 cgagatggcg ctcatcgccg acgacctcgt ggtcatcggc aagggcgccc tcatcttcca
     2041 aggaccggtc gacgcgttcg tcgacagcgc agcgaagaag tgggtgaccg tccgtagccc
     2101 acagctgcag cagatcggcg acgccctcca caacggcggc gcccaggtcg tcgccgccgg
     2161 gccgggcgcc atcgacgtgg tcgggccgac cgcggcccag atcggcgagc tcgccgccag
     2221 cctcggcgcc accctccacg agctctcccc acgcacggcg tcgctcgagg acgtcttctt
     2281 ggaggtgacc gccggcgccc aggagtaccg gggcgacacg ggggcggtcg gcgcctcggc
     2341 accaccgcct cccccgccc
//
LOCUS       DATMFS010000674         3578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_231987, whole genome shotgun sequence.
ACCESSION   DATMFS010000674 DATMFS010000000
VERSION     DATMFS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231987"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNQ33_12895"
     CDS             complement(<1..312)
                     /locus_tag="VNQ33_12895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440870.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWJ99052.1"
                     /translation="MSASETAAILHADLDSFYASVEQRDDPSLRGKPVVVGGGVVLAA
                     SYEAKAYGVHTPMGGRAARQLCPNLIEVPPRFDAYVEASRAVFDIFRETTPLVEGLSI
                     DE"
     gene            379..1353
                     /locus_tag="VNQ33_12900"
     CDS             379..1353
                     /locus_tag="VNQ33_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWJ99053.1"
                     /translation="MPTATPPELTPAQQRTLADLGAGGGDRPVFPSGLRERLRSVLEA
                     ELAPVLGDLGPDELLTLSKHLLSQVHGCEARFLAELDAPFVPSVPIVRGTVAHKAIEL
                     GIHWRGEPIPLELVDEAIARLTESDLWMAGFLQGCTEAELAELRASSGDRVSKFFECF
                     PPLKPVWRPVTESSQYVELLDARVVLRGKVDLTLGTATASELGNHAGKVVIDLKTGAP
                     NARHRDDLRFYALLDTLRLGVPPRLVASFYLDLGEAQTERVTEAILDAAAARTVDGAK
                     RMAALQTGAVAPVYRPSFGCRWCPALGGCDTGARWLTDEADADDGSAA"
     gene            complement(1354..2022)
                     /locus_tag="VNQ33_12905"
     CDS             complement(1354..2022)
                     /locus_tag="VNQ33_12905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ99054.1"
                     /translation="MRTHRLLVAVCLACSPVLVGCSSGAPDPASSEPQAVATTSAPET
                     GDALDVRIVADSEVPDEVAALLDAEPGIAVSSAVSEHGATMAELDAAADAALAEHPDV
                     LVYAGGTNDLPDGPVAVLAGLDERLRRYGEQACVVVAVPVFRYERGTAAEVQQRTEGT
                     RVLERAVVASGARVASYLDVALAMDQAGEDFFAEGELGDLHPGSRSYPRIAAAIAEQV
                     RACG"
     gene            2095..2994
                     /locus_tag="VNQ33_12910"
     CDS             2095..2994
                     /locus_tag="VNQ33_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWJ99055.1"
                     /translation="MADEDERDEPQRPRTATAEIVPLHLPPVADEEPAAGHDPRSGDV
                     DEWGRSERVRGWVRKAYDPMYRNWFRAEWEGLDKIPATGGALLVANHAGAIPSDAPTI
                     MHGVETELERPVYGLADHFFKSAPVVGTLWSRTGGVVAHPDNAYRLLHDQQQLVLVFP
                     EGSKGPGKTYNERYRLRRFGRGGFVEIAMRAGVPVIPIAVVGAEESMPILFKVPSVAR
                     ALGAPYFPVTANMAFGPLGALVHFPAKFKLRVLDPVTFPDVEPDQPRYSRSRIMDESE
                     AIRQQIQEALFDMLRQRRSVWFG"
     gene            3010..>3578
                     /locus_tag="VNQ33_12915"
     CDS             3010..>3578
                     /locus_tag="VNQ33_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWJ99056.1"
                     /translation="MGKRVLITGLSTFWGGRVAQALEADPDVEVIIGLDTREPTVKLE
                     RTEYVRSDENYSILSRIVKATGVDTIAHTFLVVDSTTMSSRAMHEINVIGTMNLFAAA
                     SAAGSTVRKVVVKSAALVYGSSPKDPYWFRETDRRVAPARTRVERSLLEVESYVRDFA
                     RDNPHVTLSLLRFCNVVGPDITTPLTKALR"
BASE COUNT          497 a         1374 c         1235 g          472 t
ORIGIN      
        1 ctcgtcgatt gacagcccct ccaccagcgg cgtggtctcg cggaagatgt cgaacacggc
       61 cctgctggcc tcgacgtagg catcgaaccg gggcgggacc tcgatcaggt tcgggcagag
      121 ctggcgggca gcccggccgc ccatcggggt gtgcacgccg taggccttgg cctcgtagct
      181 ggccgccagc accacgccgc caccgaccac gaccggcttc ccgcgcaacg acgggtcgtc
      241 gcgctgctcg accgacgcgt agaaggagtc caggtcggcg tggaggatcg cggcggtctc
      301 ggaggcggac atcgaacaca tgttcgcaca ccgtcgtccg gctgtcaccc gccggtgcgg
      361 ggcgtacgct cccccgccat gcccaccgcc acgccgcccg agctgacgcc ggcgcagcag
      421 cggaccctcg ccgacctggg ggccggcggc ggcgatcggc ccgtgttccc ctcgggcctg
      481 cgcgagcggc tccggtcggt cctggaggcc gagctggccc ccgtcctggg cgacctcggg
      541 cccgacgagc tcctgacgct ctccaagcac ctgctctcgc aggtccacgg ctgcgaggcc
      601 cggttcctgg ccgagctcga tgccccgttc gtcccgtcgg tcccgatcgt ccgggggacg
      661 gtggcccaca aggcgatcga gctgggcatc cactggcggg gcgagccgat cccgctcgag
      721 ctggtcgacg aggccatcgc ccgcctcacc gagtccgacc tctggatggc cgggttcctc
      781 cagggctgca cggaggcgga gctggccgag ctccgggcct cgtcaggcga ccgggtgtcg
      841 aagttcttcg agtgcttccc cccgctcaag cccgtgtggc ggcccgtcac cgagagcagc
      901 cagtacgtcg agctcctcga tgcccgggtg gtgctgcgcg gcaaggtcga cctgacgctc
      961 gggacggcca cggcgagcga gctgggcaac cacgccggca aggtcgtcat cgacctcaag
     1021 accggggctc cgaacgcccg ccaccgagac gacctgcggt tctacgccct gctcgacacg
     1081 ctgcgcctcg gcgttccccc gcggctggtg gcctccttct acctggacct cggcgaagcg
     1141 cagacggaac gggtgaccga ggcgatcctc gacgccgccg cggcacgcac cgtcgacggc
     1201 gccaagcgca tggccgcgct ccagaccggg gcggtggccc cggtgtaccg gccgagcttc
     1261 ggctgccggt ggtgccccgc gctcggcggc tgcgacacgg gcgcgcgctg gctcaccgac
     1321 gaggccgacg ccgacgacgg ctcggccgcc tgatcagccg caggcgcgga cctgctcggc
     1381 gatggcggcg gcgatccggg gataggaccg ggagccgggg tggagatcgc ccagctcgcc
     1441 ctcggcgaag aagtcctcgc ccgcctggtc catcgcgagg gcgacatcga ggtacgaggc
     1501 caccctcgcc ccgctcgcca ccaccgcccg ctccaggacc cgcgtcccct ccgtgcgctg
     1561 ctggacctcg gcggcggtgc cgcgctcgta ccggaagacc gggaccgcca ccacgacgca
     1621 cgcctgctcc ccgtaccggc gcaggcgctc gtcgaggccg gcgagcaccg ccaccggccc
     1681 gtcgggcagg tcgttggtcc ccccggcgta cacgagcacg tcggggtgct cggccagggc
     1741 cgcatcggcc gccgcgtcga gctcggccat cgtcgccccg tgctcggaga ccgcggacga
     1801 caccgcgatg ccgggctccg cgtcgagcag cgccgccacc tcgtcgggga cctcgctgtc
     1861 cgccacgatg cggacgtcga gagcgtcgcc ggtctcgggc gccgaggtcg tggccaccgc
     1921 ctgcggctcg ctcgacgccg ggtcgggggc gccggagctg cacccgacca ggaccggcga
     1981 gcacgccagg cacaccgcga cgaggagacg gtgggtccgc acggcggcga ggctacggca
     2041 gcggccgcac cacggggaag gcgcctgacg gggccgcggt agcctgtggc ccctatggcc
     2101 gacgaggacg agcgcgacga accgcagcga ccgcggaccg ccacggccga gatcgtcccg
     2161 ctccacctcc cgccggtcgc ggacgaggag ccggccgccg ggcacgaccc ccgctccggc
     2221 gacgtcgacg agtggggccg atccgaacgg gtgcgcggct gggtgcgcaa ggcctacgac
     2281 ccgatgtacc ggaactggtt ccgggccgag tgggagggcc tcgacaagat ccccgccacc
     2341 ggcggcgccc tgctggtggc caaccacgcc ggtgccatcc catccgacgc gcccacgatc
     2401 atgcacggcg tcgagaccga gctcgagcgg ccggtgtacg ggctcgccga ccacttcttc
     2461 aagagcgcgc cggtcgtcgg caccctctgg tcccgcaccg gcggcgtcgt cgcccacccc
     2521 gacaacgcct accggctcct ccacgaccag cagcagctcg tcttggtctt ccccgagggc
     2581 tcgaagggcc ccggcaagac ctacaacgag cgctaccggc tgcgccgctt cggccggggc
     2641 ggcttcgtcg agatcgccat gcgcgccggc gtgcccgtca tccccatcgc cgtggtgggc
     2701 gccgaggagt cgatgccgat cctgttcaag gtgccgtcgg tcgcccgggc gctcggggcg
     2761 ccgtacttcc ccgtcaccgc caacatggcg ttcggcccgc tcggcgccct cgtgcacttc
     2821 ccggccaagt tcaagctgcg ggtgctcgat ccggtcacct tccccgatgt cgagccggac
     2881 cagccgcggt actcccgcag ccggatcatg gacgagtccg aggccatccg ccagcagatc
     2941 caagaggcgc tgttcgacat gctgcgccag cgccgctcgg tctggttcgg gtgagcggga
     3001 gccagcggca tgggtaagcg ggtgctcatc accgggctgt ccacgttctg gggcggccgc
     3061 gtcgcccagg ccctcgaggc cgatcccgac gtcgaggtca tcatcggcct cgacacccgc
     3121 gagcccacgg tgaagctcga gcgcaccgag tacgtgcggt ccgacgagaa ctactcgatc
     3181 ctctcgcgga tcgtgaaggc caccggcgtc gacaccatcg cccacacgtt cctggtggtc
     3241 gactccacca cgatgtcgtc gcgcgccatg cacgagatca acgtcatcgg caccatgaac
     3301 ctcttcgccg ccgcctcggc tgcgggcagc acggtccgca aggtcgtcgt gaagtcggct
     3361 gccctcgtct acggctcgtc gcccaaggac ccctactggt tccgggagac cgacaggcgg
     3421 gtggcgccgg cacggacccg cgtcgagcgc agcctgctcg aggtcgagag ctacgtgcgc
     3481 gacttcgccc gcgacaaccc gcacgtcacg ctcagcctcc tgcggttctg caacgtcgtc
     3541 ggacccgaca tcaccacgcc gctcaccaag gcgctgcg
//
LOCUS       DATMFS010000675         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_232054, whole genome shotgun sequence.
ACCESSION   DATMFS010000675 DATMFS010000000
VERSION     DATMFS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232054"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2894
                     /locus_tag="VNQ33_12920"
     CDS             50..>2894
                     /locus_tag="VNQ33_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWJ99057.1"
                     /translation="MTFTDESTAPAGIAEWKWEFGDGTTSTDPSPDHLFADSGGFTVK
                     LTITDGKGRVASTTKAVTVDPGPPSGSVDDQTASADADVFLHGRISSPSSFERRSLQV
                     DITSSASGFPKQAARRLPAGLIDVNLGRVPTGTYPVTLTVTDSAGQKATAMAVLVVVP
                     GTPPEPTEAPPVAQPLVDGCGTAVTIPGEADGLLVGLSTLRERANRLEVVASPTLMEA
                     AERHAADLAAHPDLVDTGSDGSTPAERAADAGYPDGFDVTELVLRGPKQPLDALALWQ
                     GQTEAGQKLVDRTWVGAGAARVQAGSSWVWVLVLGEAIDCGPDDATTDFARVAAVLAA
                     DAPVPEAVPVVDPQAVQPAVGPSVATADPSRGSDGPALPVAPVGEDGDASPDAAFSGS
                     GDGAATAADTILSLPKDAVPAFTVDDATPATGATVLVHNRTRRLGQLVSATFRSDDIR
                     DPIELEPGATRATSWYSAGVWKASIQATASGVSASAAVTAAGAIVTPAVAYSGPATAA
                     QGATVPVTATVTSLGSGLPLAGRTVSFTVGSASVTGTSGSDGKVRADLVIAPTTPPGS
                     TQIVTKALAVGPSPAAQVSSPFAISANGAPTADAGGPYEIPIGDGVVFDGSGSSDPNG
                     DALTFAWDVAGEGTFDADNTFTSSWRAGDIERFICKGECQVDVAYPVALRVTDVNGAS
                     DTDYTTLTMRRDFILTVNPANTVVVPGATTSYQVTVLSTSGFDLPVSLAVTGLPAGVT
                     ASLPSTALPNTTVLLSVTAASNAAETSATLTVTGTAGGLTRKAGATVDLEFGLIPQCF
                     GAVEGYVTDRETGLPIAGASVSGGGFGIHTTDADGYFKSEGLRVGNTNGPLSYQFTAS
                     KEGYWLRNQTVTIGCGITSRADASLIQVRKGTVSGRITEGTRDMSDPDSPVVSTGVPI
                     ADARVAVDKEYRTDADGRYQTDPITLGADNGPSS"
BASE COUNT          409 a         1116 c         1002 g          367 t
ORIGIN      
        1 caccgcccgg ttcgccgtcg atccggcgac ggggaaggcg ggggcgccga tgaccttcac
       61 ggacgagtcg acggcgcccg ccggcatcgc cgagtggaag tgggagttcg gggacgggac
      121 gacctccacc gaccccagcc ccgatcacct cttcgccgac tccggcgggt tcaccgtgaa
      181 gctcacgatc accgatggga agggccgcgt ggcgtcgacg accaaggcgg tcacggtcga
      241 tccgggcccg ccctcgggct ccgtcgacga ccagacggcg tccgccgacg ccgacgtgtt
      301 cctccacggc cgcatcagct cgccgagctc cttcgagcgc aggtccctcc aggtcgacat
      361 cacgtcgtcg gcctccggct tcccgaagca ggcggcccgc cgcctccccg ccggtctgat
      421 cgacgtcaac ctggggcggg tccccacggg cacctacccc gtcaccttga ccgtcaccga
      481 ctccgcgggc cagaaggcca cggcgatggc cgtcctcgtg gtggtccccg gcaccccgcc
      541 ggagccgacc gaggccccgc cggtcgcgca gccgctggtc gacgggtgcg gcaccgcggt
      601 gaccatcccg ggcgaggccg acgggctgct cgtcgggctc agcaccctcc gcgagcgggc
      661 caaccggctc gaggtcgtcg cctcgccgac cctcatggag gccgccgagc gccacgccgc
      721 cgatctggcg gcccatcccg acctggtcga caccggcagc gacggcagca cgcccgccga
      781 gcgcgccgcc gacgccggct accccgacgg cttcgacgtg accgagctcg tgctccgggg
      841 tccgaagcag ccgctcgacg cgctcgcgct ctggcagggc cagacggagg ccggtcagaa
      901 gctcgtcgat cggacctggg tcggcgcggg cgcggcgcgg gtccaagccg gcagctcgtg
      961 ggtgtgggtg ctcgtcctcg gtgaggcgat cgactgcgga cccgacgacg ccacgacgga
     1021 cttcgcccgt gtcgcggcgg tgctggccgc cgacgcgccc gtccccgagg ccgtgcccgt
     1081 ggtcgatccg caggcggtcc agcccgccgt cggcccctcc gttgcaacgg cggatcccag
     1141 ccgggggagc gatggacccg cgctcccggt ggccccggtc ggcgaggacg gcgatgcatc
     1201 tccggatgcc gccttctccg gctccggcga cggtgcggcc acggcggccg acacgatcct
     1261 cagcctgccc aaagacgctg tcccggcgtt cacggtggac gacgcgacgc cggcgaccgg
     1321 tgcgacggtc ctggtccaca accgcacgcg gaggctgggc cagctcgtct cggccacgtt
     1381 ccgctccgat gacatccgtg accccatcga gctcgaaccc ggcgcgaccc gtgccacgtc
     1441 gtggtactcc gccggcgtct ggaaggcgtc gatccaggcc acggcgtcgg gtgtctccgc
     1501 gtcggccgcg gtgaccgccg caggagccat cgtcaccccg gccgtcgcgt acagcggccc
     1561 ggcgaccgcg gcccaaggcg ccacggtgcc ggtgaccgcc acggtcacgt cgctcggcag
     1621 cggcttgccg ctcgccggac gcaccgtcag cttcaccgtc ggctcggcat cggtcacggg
     1681 cacctccggg tcagacggca aggtccgggc cgacctggtg atcgcgccca cgaccccgcc
     1741 gggcagcacc cagatcgtga ccaaggcgct cgccgtcggg ccgagcccgg ccgcgcaggt
     1801 ctcgtccccc ttcgccatca gcgcgaacgg cgccccgacc gccgatgcgg gcgggcccta
     1861 cgagatcccg atcggcgatg gcgtcgtgtt cgacggcagc ggatccagcg acccgaacgg
     1921 cgatgccctc accttcgcgt gggacgtcgc cggggaaggg accttcgacg ccgacaacac
     1981 cttcacgtcg tcgtggcggg ccggcgacat cgagcggttc atctgcaagg gcgagtgcca
     2041 ggtcgacgtg gcgtacccgg tcgcgctgcg cgtcaccgac gtcaacggcg cctcggacac
     2101 cgactacacg acgctcacga tgcgccggga cttcatcctc accgtcaacc cggccaacac
     2161 ggtggtcgtg ccgggtgcga ccaccagcta ccaggtgacg gtcctctcga ccagcggctt
     2221 cgacctgccg gtgagcctcg ccgtcacggg cctgccggcg ggcgtcaccg cgtccctgcc
     2281 ctcgacggcg ctgccgaaca ccaccgtgct gctcagcgtg acggccgcgt cgaacgcggc
     2341 ggagacctcg gccacgttga cggtgacggg caccgccggc ggcctgacgc gcaaggccgg
     2401 cgccaccgtc gacttggagt tcgggctgat cccgcagtgc ttcggcgcgg tggagggcta
     2461 cgtgaccgac cgcgagaccg gcctgcccat cgccggagcg agcgtgtccg gtggcggctt
     2521 cgggatccac acgaccgatg ccgacggcta cttcaagtcg gagggcctcc gtgtcggcaa
     2581 cacgaacggt ccgctcagct accagttcac cgcgagcaag gagggctact ggctgcggaa
     2641 ccagaccgtg acgatcgggt gcggcatcac gagccgcgcc gacgcctcgc tcatccaggt
     2701 ccgcaagggg accgtcagcg ggcggatcac cgaaggcacc cgtgacatga gcgatccgga
     2761 cagccccgtc gtctcgaccg gggtgccgat cgccgacgcc cgggtcgcgg tcgacaagga
     2821 gtaccgcacc gacgccgacg gccgctacca gaccgatccg atcacgctcg gcgcggacaa
     2881 cggtcccagc tcgg
//
LOCUS       DATMFS010000676         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_185361, whole genome shotgun sequence.
ACCESSION   DATMFS010000676 DATMFS010000000
VERSION     DATMFS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185361"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VNQ33_12925"
     CDS             complement(<1..1029)
                     /locus_tag="VNQ33_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99058.1"
                     /translation="MLLARVGLFLLSLWLIQRCLPPIRATDYLPTSGTGDDRWWSAVL
                     ILGVSSFTGCFLLFIGLAVAILPASSGPWSWRAVLTVGAVGLCALAVQSIWQVVRSGE
                     LGRVAERRWREDVDAASVQLLSDLDVHWRDANAMPIVLGERASGSGAPLPPVAAQLER
                     HKKRLEEWNKGAKKVLDKLKTIDGEVVAAVDAAVARAAAGGAKPDVGAGEPPSPLPVE
                     VPGDSILQVVEAGGEEPHPGRPHKLTRDALEGRTTVSLWYATKEQVEKEIVGARAKLD
                     EPRSRMLGHAAEFRAGRLGAVADVLRRTAMLTDRLRFAWRVHPLSDTARDDSKVRGVL
                     ISPETFGAF"
     gene            complement(1158..2624)
                     /locus_tag="VNQ33_12930"
     CDS             complement(1158..2624)
                     /locus_tag="VNQ33_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99059.1"
                     /translation="MAVRRAFEYSAKVASLGYTLRPNLALHLELIKAIGPADLVLPGT
                     GEADENALFLYERSVETAVLSLSWGARSWSNINQDDVKQTAAALYDHLGTLYRALPAG
                     AQKKLYEVVAPIYTRLDLLRQGGQARVDDESRREIKRLRNADPGTAAELALELGDGIT
                     AAQVEEWKALETSVAEAEKSVDKPNPTEAEIEQYHQRLDRFLKSTVTQKILQKAGVKD
                     AKKVQAAASCVAGVILNGANLIARWDKLNTPQKALEVSKLLVGAGLAIQAFMSASTAG
                     VKTGILLLLVQLLDFIDLGINLGIDLFPGGGKKNDGGGEGEGKGQGPGAGKKDEKSEK
                     SEKPGGGGAPEPEPQRHPEDKGSGFKGTLKADKPCEAKPSDGKLEQAQKKAKDDFNKK
                     LDDAKKKAGTEKEKKEIETAKLPTTQAPKHDFEQHLKERGIDPDKDLDKAAALLNEWR
                     DAIGEALITNVPPRVKAKADALPSRSGRGDRSGNDPQP"
     gene            complement(2840..>3196)
                     /locus_tag="VNQ33_12935"
     CDS             complement(2840..>3196)
                     /locus_tag="VNQ33_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99060.1"
                     /translation="RSRSTPEKEFRAFLDQYPGTAGRPVVWSTINYSLRSLDDELKRI
                     TGDPALRRRLVAAFGLRRLRVGLPLVAVGLGLAGACAFTVVVAGGSMAATLNLMLIPG
                     LCLAALGIFRFYLSPL"
BASE COUNT          432 a         1105 c         1117 g          542 t
ORIGIN      
        1 gaacgccccg aacgtctcgg ggctgatcag gaccccgcgc accttcgagt cgtccctcgc
       61 cgtgtccgac agggggtgga cccgccaggc gaatcggagc cggtcggtca gcatcgcggt
      121 ccgcctcagg acgtcggcga ccgcaccgag ccgcccagcg cggaactcgg cggcgtggcc
      181 gagcatcctc gaccggggct cgtcgagctt ggccctggcg ccgacgatct ccttctccac
      241 ctgctccttg gtcgcgtacc agagcgagac ggtcgtgcgg ccttcgaggg cgtcgcgggt
      301 cagtttgtgc ggtcggcccg ggtggggctc ctcgccgccg gcctcgacga cctggaggat
      361 ggagtcgccg ggcacctcaa ccggaagggg cgacgggggc tcaccggccc cgacgtccgg
      421 cttcgcgccg ccggccgcgg ccctggcgac cgccgcgtcg acggcggcca cgacctcgcc
      481 gtcgatggtc ttgagcttgt cgaggacctt cttcgccccc ttgttccact cctccagccg
      541 cttcttgtgc cgctcgagtt gggcggcgac cggcggcagc ggcgccccgg acccgctggc
      601 gcgctcaccc aagacgatcg gcatggcatt ggcgtcgcgc cagtggacgt ctaggtcgct
      661 gagcagttgc acggacgccg cgtcgacgtc ctccctccag cggcgctcgg cgacccggcc
      721 cagttccccc gagcggacta cctgccagat cgactggact gccagggcgc atagcccgac
      781 agccccgacg gtcagcacgg cacgccagct ccagggcccc gacgaggccg gcaggatcgc
      841 caccgccagc ccgatgaaca gcaggaaaca tccggtgaag ctgctgacgc cgaggatcag
      901 cacggcgctc caccaccggt cgtcgccggt cccggacgtc ggcaggtagt ccgtcgcccg
      961 gatcggcggc aggcaacgct gaatcagcca gaggctcagc aggaacagcc cgaccctggc
     1021 cagcagcatg ctcaggtggc gaacgacgtt catggcgggg tccggtcagg gttgagggtc
     1081 gaggttcgga cgtcacgctg cgacgactct tcgtgcggcg ccggcggccc cggctgccgg
     1141 ctccgcacgc ggcatggtta cggctgcggg tcgttcccgc tgcggtcgcc ccgccccgac
     1201 ctggacggta aggcgtcggc cttcgccttg actcggggcg gcacgttcgt gatcagggcc
     1261 tcgccgatcg cgtcgcgcca ctcgttcagg agcgcggcgg ccttgtccag gtccttgtcc
     1321 gggtcgatcc cccgctcctt caggtgttgc tcgaagtcgt gtttcggggc ctgggtcgtg
     1381 ggcagcttcg ccgtctcgat ctccttcttc tccttctcgg taccggcctt cttcttggcg
     1441 tcgtcgagct tcttgttgaa gtcgtccttc gccttcttct gcgcttgctc gagtttccca
     1501 tcactcggct tggcctcgca gggcttatcg gccttgaggg tgcccttaaa gcccgacccc
     1561 ttgtcctcag ggtggcgctg gggttcgggt tcgggggcgc cgcctccgcc gggcttctcc
     1621 gacttctcgg acttctcgtc cttcttgccg gcgcccgggc cctggccctt gccctcccct
     1681 tcgcctcccc cgtcattctt cttgccgccg ccggggaaca ggtcgatgcc gaggttgatg
     1741 ccgaggtcga tgaagtccag cagctgcacc agcagcagga ggatgcctgt cttgaccccg
     1801 gcggtcgagg cggacatgaa cgcctggatc gccaggccgg ccccgacgag gagcttgctg
     1861 acttcgaggg ccttctgggg cgtgttcagc ttgtcccaac gggcgatgag gttcgcgccg
     1921 ttgaggatca cgccggcgac gcacgaggcc gccgcctgaa ctttcttcgc gtccttgacg
     1981 ccggccttct ggaggatctt ctgcgtgacc gtgctcttaa ggaagcggtc gaggcgctgg
     2041 tggtactgct cgatctcggc ctcggtcggg ttcggcttgt ccaccgactt ctcggcctcc
     2101 gcgaccgacg tttcgagcgc cttccactcc tcgacctggg ccgcggtgat gccgtcgccc
     2161 agctcgaggg cgagctcggc ggcggtgccg gggtcggcgt tgcggaggcg cttgatctcg
     2221 cgccggctct cgtcgtccac gcgggcctgc ccaccctggc ggagcaggtc gaggcgggtg
     2281 tagatcggcg cgacgacctc gtagagcttt ttctgggccc cggccggcag agcgcggtag
     2341 agggtgccaa ggtggtcgta cagggcggcg gcggtctgct tgacgtcgtc ctggttgatg
     2401 ttgctccacg agcgggcgcc ccaggagagg gagaggaccg ccgtctcgac ggaacgctcg
     2461 tagaggaaca gggcgttctc gtccgcctcg cccgtgccgg ggagaacgag gtcggcgggg
     2521 ccgatcgcct tgatcagctc caggtggagc gccaggttgg ggcggagggt atagccgagc
     2581 gaggcgacct tcgccgagta ctcgaaggcg cggcggactg ccatcgactt gcacgcctcg
     2641 taacgctgcc gcgcccgcga ctgagcgtcc tggacccagg gcaactggac tgtcgggttg
     2701 tcgttagatt ggggcggcga ctgagcgtgg gccgcgcggg ggatggcgag gagaggccca
     2761 acggccaccg ccagcgccac cgcggcgcaa accgtctttc gaagccggat gtgtagcaaa
     2821 gcgagactcc ttccgaggtt cagagtgggg aaaggtagaa gcggaaaatc ccgagcgcgg
     2881 ccaggcacag gccgggtatc agcatcaggt tcagggtcgc ggccatcgac ccgcccgcca
     2941 cgaccacagt aaaggcgcag gcccccgcca ggcccagccc caccgctacc aacggaaggc
     3001 cgacgcggag gcgtcgcagc ccgaacgccg cgacgaggcg gcggcggagg gctgggtcgc
     3061 cagtgatccg cttcagctcg tcgtcgaggc ttcgcaaact gtagttgatc gtcgaccaga
     3121 cgaccggccg cccggcggtg cccgggtact ggtcgaggaa cgcgcggaac tccttctcgg
     3181 gcgtcgaccg cgagcg
//
LOCUS       DATMFS010000677         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_185421, whole genome shotgun sequence.
ACCESSION   DATMFS010000677 DATMFS010000000
VERSION     DATMFS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185421"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VNQ33_12940"
     CDS             <1..1286
                     /locus_tag="VNQ33_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ99061.1"
                     /translation="VVVLEAADRAGGLASSTVDDAGFTWDRGGHVVFSHYGAFDELLA
                     EVLGAEVHHHDRSSWVLAGDTWVPYPFQNNLHRLAPEAAEACLVGLIEAGAGGRRPDP
                     GSDLGSWAAATFGAGVVEHFFSPYNQKVWASALEDLSSSWIAERVAVVDWRDALRRTL
                     RGRDDVNWGPNDQFAFPARGGTGEIWRRAAEPFATAIRYGERVERVESADHVVVTAGG
                     TEVGYRHLISTMPLDRLVAVVTSPPEDVAAAAASLVHTSVHVVGMGYEAPTTDDRSWL
                     YFADPEVPFYRATNFGKYAAANLPAADDQRYCSWMCEVSSSEARPTSSDGLVDAVDAA
                     LRRTGVVAGDPVVATRHLETVPYAYPVPTRGRDAALAVALPWLDARSIWSRGRFGTWR
                     YEVGNMDHAVKMGIDAAQRVATGAPEALVPRELVA"
     gene            1283..1996
                     /locus_tag="VNQ33_12945"
     CDS             1283..1996
                     /locus_tag="VNQ33_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ99062.1"
                     /translation="MSDPVELSVVLPAHDEEAAIEGVIRDVVATVRSLAPGASEVLVV
                     DDGSTDATGQILDRLAAELAEVEVVHQRPNYGHGPALLTGFARARGAWVGHLDTDDQI
                     PADQLAQLWTRRAGADLVLGVRVARDDPRHRLVVTKVLRFLVGRFAGRPIRDANVPCK
                     LISRDLLEEALPLLPADTFAPSVGLAVVAARRGRTIVEVPVVHQARPHGESSLKPLRL
                     VSALVTSTRQTAALARSLR"
     gene            complement(2051..>3400)
                     /locus_tag="VNQ33_12950"
     CDS             complement(2051..>3400)
                     /locus_tag="VNQ33_12950"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HWJ99063.1"
                     /translation="AWCRVSHTWDLPTLALLSVGAVVVGTLLDPTAGAQRWERWKRGA
                     LHLGVIGLTTLVVSHPYAAKGQVFDSGFTGSPVHTPFGSFLLQYGIAFGVVTVALVTR
                     TIQLRRHLGLPPVLRSRAGRSGVLAMAVGGGLLAALAGGPVTVLCAGLVACSLALAAH
                     ELRARRLGSGLAWALVAAGAGLAALPELVVIVNDIGRQNTVFKFGMTSWSLLALGAAG
                     TAATAVPGARFRRSTMAVIALLVIPGLVFWPSAVGPRLDARFQPLAATLDGRAWLDHG
                     PVVVEANEVPPIDVTQDDDLIAWMRAHVPGGATIVEAAGPSYTWVGRVSVATGLPTVI
                     GWAFHETQQRRGYANEVDERTAAVKRLYADPEPVQALRTLAAYRPDYVVVGTVEHALG
                     TPEAIDALGGLPGIEVVWQHGDEVLYEVDHPAVDRALAAIDAERIAAGEFAPDDAGS"
BASE COUNT          429 a         1223 c         1304 g          444 t
ORIGIN      
        1 acgtcgtcgt gctggaggct gccgatcgag cgggtggcct ggcgtcgtcc accgtcgatg
       61 atgccggctt cacgtgggac cgagggggtc acgtcgtgtt cagccactac ggcgccttcg
      121 acgagctgct cgccgaggtc ctcggtgccg aggtgcacca ccacgaccgc tcgtcgtggg
      181 tgctcgccgg tgacacgtgg gtcccgtacc cgttccagaa caacctgcac cgactggcgc
      241 ccgaggcggc cgaggcctgc ctcgtgggcc tcatcgaagc gggagccggg ggccgccgcc
      301 ccgatcccgg ttcggacctc gggtcgtggg ccgcggccac gttcggcgca ggggtggtcg
      361 agcacttctt ctcgccgtac aaccagaagg tgtgggccag cgcgctcgag gacctgagct
      421 cgtcgtggat cgccgagcgg gtggccgtgg tggactggcg cgacgccctg cgccggacgc
      481 ttcgcggccg tgacgacgtc aactggggac cgaacgacca gttcgccttc ccggctcgtg
      541 gcggcaccgg ggagatctgg cggcgcgccg ccgagccgtt cgccaccgcg atccgctacg
      601 gcgagcgcgt cgagcgcgtc gagtccgccg accacgtcgt cgtcaccgcc ggcggcaccg
      661 aggtcgggta ccggcacctc atctccacca tgccgctgga ccggctggtc gcggtcgtga
      721 cgtcgccgcc cgaggatgtc gcggcggcgg cggcctcgct cgtccacacg agcgtgcacg
      781 tcgtgggcat ggggtacgag gcccctacga ccgacgaccg gtcgtggctc tacttcgccg
      841 accccgaggt gcccttctac cgggccacca acttcggcaa gtacgccgcc gccaacctgc
      901 ccgctgccga cgaccagcgg tactgctcgt ggatgtgcga ggtgtcgtcg tcggaggccc
      961 gtcccacctc gtcggacggg ctggtcgacg ccgtcgacgc cgccctgcgg aggaccggcg
     1021 tcgtggccgg tgacccggtc gtggccaccc ggcacctgga gaccgtgccg tacgcgtacc
     1081 cggtcccgac gcgaggacgc gacgccgcgc tggccgtcgc cctgccctgg ctcgatgccc
     1141 gcagcatctg gtcgcggggg cggttcggga cctggcgcta cgaggtcggc aacatggacc
     1201 acgccgtgaa gatgggcatc gacgccgctc agcgcgtggc gaccggggct cccgaggcgc
     1261 tcgtgccccg ggagctggtg gcatgagcga cccggtcgag ctgtcggtgg tgctgcccgc
     1321 gcacgacgag gaggcggcga tcgagggggt gatccgcgac gtcgtcgcca cggtccgctc
     1381 cctggcgccc ggcgcgtcgg aggtgctggt ggtcgacgac ggctccaccg acgccacggg
     1441 ccagatcctc gaccgcctgg ccgccgagct cgcggaggtg gaggtcgtcc accagcgacc
     1501 caactacggc cacgggccgg cgctgctcac cgggttcgcc cgcgctcgtg gcgcgtgggt
     1561 cggccacctc gacaccgatg accagatccc cgccgaccaa ctggcgcagc tctggacccg
     1621 gcgcgccggg gcggatctcg tgctcggggt gcgggtggcc cgcgacgacc cgcggcaccg
     1681 gctggtcgtg accaaggtgc tgcgcttcct ggtgggccgc ttcgccgggc gaccgatccg
     1741 cgacgccaac gtgccgtgca agctgatctc gagggacctg ctcgaggagg cgctgcctct
     1801 cctgcccgcg gacacgttcg ccccgtcggt ggggctggcg gtggtggccg cgcgccgggg
     1861 ccggaccatc gtcgaggtcc cggtggtcca ccaagcccgc ccccacggcg agtccagcct
     1921 caagccgctg cgcctggtgt cggccctggt gacctcgacc cgccagaccg ccgccctcgc
     1981 ccgctcgctg cgctgagcgg tccctccgcg ctcgggcggt gggtgcggtg cggtgtcggg
     2041 cgcggtccgg tcagctgccg gcgtcgtcgg gggcgaactc gccggcggcg atgcgctcgg
     2101 cgtcgatggc ggcgagcgcc cggtcgaccg cggggtggtc gacctcgtag aggacctcgt
     2161 cgccgtgctg ccacacgacc tcgatgccgg ggaggccgcc cagcgcgtcg atcgcctcgg
     2221 gcgtgcccag ggcgtgctcc acggtgccga cgacgacgta gtccggccgg taggcggcga
     2281 gggtgcggag ggcctggacg ggctcggggt cggcgtagag gcgcttcacc gccgcggtcc
     2341 gctcgtcgac ctcgttggcg tagccgcgcc gctgctgggt ctcgtggaac gcccagccga
     2401 tcacggtcgg caggccggtg gcgaccgaca cccggcccac ccacgtgtag ctgggccccg
     2461 ccgcctcgac gatggtcgct ccgccgggca cgtgggcgcg catccaggcg atgaggtcgt
     2521 cgtcttgggt gacgtcgatg ggcgggacct cgttggcctc caccaccacc gggccgtggt
     2581 cgagccaggc ccggccgtcg agggtggcgg ccaggggctg gaagcgggca tcgagcctcg
     2641 ggccgaccgc ggagggccag aacaccagtc cggggatcac caggagggcg atgacggcca
     2701 tcgtggaccg gcggaaccgg gcacccggca cggcggtagc ggcggtgccg gcggcgccca
     2761 gggcgaggag cgaccacgag gtcatcccga acttgaagac cgtgttctgc cggccgatgt
     2821 cgttcacgat gacgaccagc tcggggaggg cggccagccc ggcgccagcg gcgacgagcg
     2881 cccacgccag gccgctgccg aggcggcggg cgcgcagctc gtgcgcagcg agggcgagcg
     2941 agcaggcgac gagcccggcg cacagcacgg tgaccgggcc gcccgcgagc gcggccagga
     3001 gcccgccgcc gacggccatc gccagcacgc ccgagcgccc ggctcggctc cggagcacgg
     3061 gtggcagccc gaggtggcgg cggagctgga tcgtccgggt cacgagggcg acggtcacga
     3121 cgccgaaggc gatgccgtac tgcaggagga agctgccgaa gggcgtgtgg accggcgagc
     3181 cggtgaagcc cgagtcgaac acctggccct tggcggcata ggggtggctc accacgaggg
     3241 tcgtcaggcc gatcaccccg aggtggagcg cgccgcgctt ccaccgctcc caccgctggg
     3301 cgccggctgt cggatcgagg agggtgccga cgacgacggc gcccacgctg aggagcgcca
     3361 gggtcgggag gtcccaggtg tggctcaccc ggcaccaggc
//
LOCUS       DATMFS010000678         9540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13469
            k141_185543, whole genome shotgun sequence.
ACCESSION   DATMFS010000678 DATMFS010000000
VERSION     DATMFS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942872
            Sequence Read Archive: SRR7976237
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:29:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,600
            CDSs (total)                      :: 2,575
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185543"
                     /isolate="SMAG_U13469"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.83 N 122.29 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VNQ33_12955"
     CDS             complement(<1..337)
                     /locus_tag="VNQ33_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ99064.1"
                     /translation="MTALAGLALASALALTGCSGAASQAGGSDDKFTVAAFTAGYGTP
                     VGKATLDAFVEKGKSLGWDVKTYTSEFDYDKINTDVNGAITQGVDAILAGFPDPRQIS
                     PLVNAAKDAN"
     gene            complement(403..1260)
                     /locus_tag="VNQ33_12960"
     CDS             complement(403..1260)
                     /locus_tag="VNQ33_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ99065.1"
                     /translation="MSTSETVEKNAVETAPGDDRVAGLFSLRGDIVVVTGASSGIGQA
                     AAEIYAAAGAVVVVVGRHEGRLAAVRDGIIAAGGLAHSYVADVAVRGAFDRVLADVVA
                     EVGQPTVIVANAGIAGGGSYLTDEGSLANYTDGEWDSVIATNLTGTFETLRAAARVLP
                     DGGRILVTASTAGLRTDPMVSYAYVTTKAAILNLVRQMALEFAPRGIRVNAIAPGPFK
                     DTRIGGGAVLDAEGERQWSATIPLGRMGVMEQIQGPMLFLVAPGSAFITGATLAVDGG
                     ALALSHTLF"
     gene            complement(1369..2343)
                     /locus_tag="VNQ33_12965"
     CDS             complement(1369..2343)
                     /locus_tag="VNQ33_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWJ99066.1"
                     /translation="MAALAGLALASALALTGCSGSGSANGTGGGAEDTFKVAAFTAGY
                     GTPGGKSTLDDFVKKGEALGWDVTLYTSEFDYDKINTDVNGAITQGVDAILAGFPDPR
                     QISPLVNAAKDANIPIFSIDGGVQPNDDFVVDVTFKQQQLADLTVGALEEAMGGLQGK
                     NVMVIGHDPHVGIMTRSRLAEEKLKAGGANIAGGEIKQVLNPGTSQEEALKFVTDYLQ
                     ANPDGLDGVWVGWDHAALGATQAINEAGRDDIFVTGVDALGIAANEIKKDGPFYASVF
                     QDWNSVVDEVVADMKAYDESGAKPAENFVEVEGTLIDRSNVDGFTPTN"
     gene            complement(2432..3430)
                     /locus_tag="VNQ33_12970"
     CDS             complement(2432..3430)
                     /locus_tag="VNQ33_12970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ99067.1"
                     /translation="MTKTDTLPATTTAAAAPARERTPLSQLFWQSGTIVALIALIVFF
                     TVMRPDVFLSFGNMRNILEQIAILTIIAVGQTVVMVVGDFDLSVGTTATLSGATAATL
                     MIGGTPIPVAIIAGLCVGILVGMVNGFLVAFLQLSAFVATLATMTTVGGLAFIATSGT
                     TLYGMPEDFFWIGQGRPMGIPMPIFFAVAAALILWVVLRFTTLGRKWYAVGGNAEVAR
                     LSGVNVRWTRFFAFVVAGLASAIGGIILVSRLGSASSASGNNYMMLAVAAVFLGMTIL
                     KSGQANVGGTLVGVGIIGVMSNGLNIIGVNTYVQQVLTGLIIIAAVTLSALKARKA"
     gene            complement(3432..4967)
                     /locus_tag="VNQ33_12975"
     CDS             complement(3432..4967)
                     /locus_tag="VNQ33_12975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWJ99068.1"
                     /translation="MTALKPASTGTALLEPAAALEARGIVKRFDGVHALNGASLSVQP
                     GEIHALLGENGAGKSTLIKVVTGLYTPDAGQIVRNGEELEFANVRAANKAGVVALYQE
                     LSIIPTISVAENILLGEQMPSRGSLVSWGALRKRAQEQLDRLNQRIPLGKLAGQLSPV
                     QQTMVSVARALATDARVLILDEPTAALTDLEITDLFAVLRGLRAEGVAIIYVSHRLEE
                     VFELCDRLTIMRNGETITTKAVADTTVDEVISTMVGRSAGELFPERSTATDRVVLAVE
                     GLNGRRVKDVSFQAYAGEVLGIGGLAGSGRSELLRLLAGAQKHSSGTIAVAGERLPHA
                     PGVGRVLEAGIALVPEERRSQGVIVSAPIEDNIALANLKAVSSLGWVSDRKVVDLARD
                     GLADLQIKARGPRQAVGQLSGGNQQKVVLAKMLARKPKVLLMDEPTRGIDVGTKAEIY
                     RLIRELAAGGTTVVAVSSELPELIGMSDRIIIMHEGRIAGEVQAEGADDELLLSYCYG
                     KTD"
     gene            complement(4964..6034)
                     /locus_tag="VNQ33_12980"
     CDS             complement(4964..6034)
                     /locus_tag="VNQ33_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWJ99069.1"
                     /translation="MKTVAVTKIGSLRDPDEQARGRIGLVDFAEPPLGPEDVRIRVAY
                     SAICGSDPHLAEGFFGTEVPIGLGHELSGVVEALGEKAHRNGLRVGDRVAGNFLRFCG
                     TCTPCRTGQQQFCEHIQEYNRPGMAETVTWHESQVYKLPEDVSLLKGCLLEPASVAVR
                     IADKTNIKVGDRVAICGGGPIGQLALQVLKMNGATSLTLIEPIAERREMALRHGADAV
                     IDPLTEDQYRRAQEITGDLGYDVVVDASGSPRAVRGLLDIAAKGATVVYGAMYPHDFE
                     MPLNLSDYLYLKELTLTGVFVSPYAFPRALQVLPHLELDEFTQAVFDLEDAPAAFEAH
                     VSGKHPKIVIRCNRFGDGGGAA"
     gene            complement(6070..7008)
                     /locus_tag="VNQ33_12985"
     CDS             complement(6070..7008)
                     /locus_tag="VNQ33_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ99070.1"
                     /translation="MLSARSVIGSTLAELGDEYDNLWVVTPDIGATLVEFRDKFPERF
                     LDVGLAEQACVGISAGLAYDGNIPVVSGMLPFLSMRALEQVRTDVCYPNLPVKIIGTH
                     GGLVGNGGSTHYAVEDLALMCALTNMTVTSIGDPLMVGEVIRQSMTMQGPIYIRLAVG
                     KSDRVLYEPGQHEVRIGKGIVAREGTDATIFTHGTVVAQALDAAEELAKDGRSVRVVD
                     MFTLKPIDKELIVRCAAETGGRFVVLEDHLAYGGLASRVADVVADRNINLTAFERLGI
                     PQVYAGFGGDEELRNKHGYGLDATIAATRRVIAAQA"
     gene            complement(7035..7913)
                     /locus_tag="VNQ33_12990"
     CDS             complement(7035..7913)
                     /locus_tag="VNQ33_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWJ99071.1"
                     /translation="MGQMSLRTGANPPTIEELEDLAFELRSKLLHLCGTYEGAVHIGG
                     DLSAADIFTALYHYGLNVDPADIANPARDRFVLSKGHAAVCMYIAMAMRGFFSYDGIV
                     ETYGQLDSAYGMHPCKVQLPGVECSTGSLGHGLPLAVGMALSARGRGETHRVFCLLGD
                     GETGEGSVWEAAMAARSNELGNLVAVIDRNRQLMTSFAEERIVFEPYPDKWAAFGWNV
                     VNVDGHDMRQLVEALDALPPADSNRPTVIICETVKGKGVDFMERNLAWHAGSLGPADL
                     ERAMAALEASREKESV"
     gene            8259..8783
                     /locus_tag="VNQ33_12995"
     CDS             8259..8783
                     /locus_tag="VNQ33_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWJ99072.1"
                     /translation="MTGERDQRPRAPIPPEAVTEPLVETIDPENFTPRLLALLSNALV
                     WRESQELRRRYGLGTNDWRVISAAAIRPGTSATELSEFLGLNKAVISKSVNTLIDRGL
                     LLLIDGPRGSRPMYLTRAGAQMHDAMLPISMRGQEIILADLSEEDIDRLNHLLRRMLE
                     KTRELQMLENEAHV"
     gene            complement(8957..>9540)
                     /locus_tag="VNQ33_13000"
     CDS             complement(8957..>9540)
                     /locus_tag="VNQ33_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ99073.1"
                     /translation="FPSSNPFYVQTSWPGFDPEAAKKLVAEYVADGGKAEFAMTTTSP
                     PEFQKQAQVMQQMLQDVGITVTLNVSDQPTMVTEALSGNYQSQLRFAEVREEIDQQLR
                     QFFHSTSTGNNGKAGDPQVDALLDELRTPKGQERKAEIYAELQNRFAEWLPIAPLVAH
                     KNGWYVGKDVGGFPGARVGLPDPDWRLLWTTQG"
BASE COUNT         1431 a         3188 c         3320 g         1601 t
ORIGIN      
        1 tgttggcgtc cttggccgca ttcaccagcg gggaaatctg ccgcggatcc gggaagccgg
       61 ccaggatcgc gtccacgccc tgggtgatcg ccccgttcac gtcggtgttg atcttgtcgt
      121 aatcgaactc gctcgtgtag gtcttcacgt cccagcccag ggacttgccc ttctcgacga
      181 acgcgtcgag ggtggccttg ccgacgggcg tgccgtagcc ggcggtgaag gcggcgaccg
      241 tgaacttgtc atcggatcca ccggcctggc tggccgcacc ggaacagccg gtcagtgcca
      301 gcgccgaggc cagggccagt ccggcgagtg cggtcatacg cttacgcatc atttactcct
      361 tggttgctga ttgcgccgtt ggtggaatgc ccggatgccg ggtcagaaga gggtgtggga
      421 cagcgcgagc gcgccgccgt ccacggcgag cgtggcgccc gtgatgaatg cggagcccgg
      481 tgcgacgagg aagagcatcg ggccctggat ctgctccatg acgcccatgc ggcccagcgg
      541 gatggtcgcg ctccactggc gctccccctc cgcgtcgagc acggccccgc cgccgatccg
      601 ggtgtccttg aagggcccgg gggcgatcgc gttcacgcgg atcccccgcg gcgcgaactc
      661 gagggccatc tggcggacga gattgaggat cgcggccttc gtggtgacgt acgcgtacga
      721 gaccatcggg tccgtgcgaa gcccggcggt ggaggcggtg accaggatgc ggccgccgtc
      781 gggcagcacg cgggcggccg cgcgaagcgt ctcgaacgtg ccggtgaggt tcgtggcgat
      841 cacgctgtcc cactcgccat ccgtgtagtt ggcgagcgat ccctcgtccg tgaggtagct
      901 cccgccgccg gcgatgcccg cgttggcgac gatgaccgtg ggctggccga cctcggcgac
      961 gacgtcggcc aggacccggt cgaacgcgcc ccggacggcg acgtcggcga cgtaggaatg
     1021 cgcaaggccg cccgccgcga tgatcccgtc gcgcacggcc gccagccggc cctcgtgccg
     1081 gcccacgacg accaccacgg cacccgccgc cgcatagatc tccgccgcgg cctggccgat
     1141 gccgctggag gcgcccgtca cgacgacgat gtcgccgcgc aggctgaaca ggccggcaac
     1201 gcggtcgtca ccgggagcgg tctccaccgc gttcttttcc actgtctcgg acgtgctcat
     1261 gggccatgcc tctcgttggt tctcacggct tcaaatggga gggacggggg atgcgcgttc
     1321 ggcggatccc ccgcccgccg gctcggttcg ccggctcagt tcgctggctc agttcgtcgg
     1381 cgtgaagccg tcgacgttgc tgcggtcgat cagggttccc tcgacctcga cgaagttctc
     1441 ggccggcttg gcccccgact cgtcataggc cttcatgtcc gcgaccacct cgtcgacgac
     1501 cgagttccag tcctggaaga cggaggcgta gaacgggccg tccttcttga tctcgttggc
     1561 ggcgatgccc agggcgtcga cgccggtcac gaagatgtcg tcgcggccgg cctcgttgat
     1621 ggcctgcgtg gcgccgagcg ccgcgtggtc ccagccgacc cagacgccgt ccaggccgtc
     1681 ggggttggcc tgcaggtagt cggtgacgaa cttcagggcc tcctcctgcg aggttcccgg
     1741 gttcaggacc tgcttgatct cgccgccggc gatgttcgcg cctccggcct tgagcttttc
     1801 ctcggccagg cgactgcggg tcatgatgcc cacgtgcggg tcgtggccga tgaccatgac
     1861 gttcttgccc tgcaggcctc ccatggcctc ctccagggcc ccgacggtga ggtcggcgag
     1921 ctgctgctgc ttgaaggtga cgtcgacgac gaagtcgtcg ttgggctgga cgccgccgtc
     1981 gatcgagaag atcgggatgt tggcgtcctt ggccgcattc accagcgggg aaatctgccg
     2041 cggatccggg aagccggcca ggatcgcgtc cacgccctgg gtgatcgccc cgttcacgtc
     2101 ggtgttgatc ttgtcgtaat cgaactcgct cgtgtagagc gtgacgtccc acccgagcgc
     2161 ctcgcccttc ttcacgaagt cgtccagcgt ggacttgccg ccgggcgtgc cgtagccggc
     2221 ggtgaacgcg gcgaccttga aggtgtcctc cgcgcctccc ccggtgccgt tggccgaacc
     2281 cgaccccgaa cagccggtca gtgccagcgc cgaggcgagg gccagtccgg cgagtgcagc
     2341 catgcgctta cgcatcattt tctcccttga ttgtgagtgc gtgtgtgagt gcgtcgatga
     2401 gtgtggtgtg gtgccgcctg cggcgcttcg gctacgcctt gcgcgccttg agcgccgaga
     2461 gcgtgacggc cgcgatgatg atgagcccgg tgagcacctg ctggacgtag gtgttcacgc
     2521 cgatgatgtt gaggccgttg ctcatgacgc cgatgatgcc gacgcccacc agggtgccgc
     2581 cgacgttggc ctgccccgac ttcaggatgg tcatgcccag gaagaccgcg gcgacggcca
     2641 gcatcatgta gttgtttcca ctggccgagc tggcgctgcc gaggcggctg accaggatga
     2701 tgccgccgat cgcggacgcc aggccggcga cgacgaaggc gaagaagcgg gtccaccgca
     2761 cgttcacgcc ggagagccgg gcaacttcgg cgttgccgcc caccgcgtac cacttgcggc
     2821 ccagggtggt gaaccgcagg acgacccaca ggatcagcgc cgcggcaacg gcgaagaaga
     2881 tgggcatggg gatgcccatg ggccggccct gcccgatcca gaagaagtct tccggcatcc
     2941 cgtagagcgt ggtgccgctg gtggcgatga aggccaggcc gccgacggtg gtcatcgtcg
     3001 cgagggtcgc cacgaaggcg gagagctgga ggaaggccac caggaaaccg ttcaccatgc
     3061 ccaccaggat gccgacgcac agtcccgcga tgattgccac cgggatgggg gttcctccga
     3121 tcatcagcgt cgccgccgtg gcccccgaga gggtcgcggt ggtacccacg gaaaggtcga
     3181 agtcgcccac caccatcacg accgtctggc cgacggcgat gatcgtgagg atggcgatct
     3241 gttcgaggat gttgcgcatg tttccgaagc tgaggaacac gtcgggacgc atcaccgtga
     3301 agaacacgat gagggcgatc agggcgacga tcgtgccgct ctgccagaag agctgggaaa
     3361 ggggggtgcg ttcgcgcgcc ggcgccgccg ccgccgtcgt cgttgccggg agcgtgtcgg
     3421 tcttggtcat gtcagtcggt ctttccgtag cagtaggaga ggagcagttc gtcgtcggcg
     3481 ccctcggcct gcacctcgcc ggcgatccgg ccctcgtgca tgatgatgat gcggtccgac
     3541 ataccgatga gctcgggcag ttccgagctg acggccacga cggtggtgcc gccggcggcc
     3601 agttcgcgga tgaggcggta gatttccgcc ttcgtgccca cgtcgatgcc gcgggtcggc
     3661 tcatccatca gcagcacctt gggcttgcgg gcgagcatct tggcgagcac gaccttctgc
     3721 tggttgccgc cggagagctg gccgacggcc tgccgcggcc cgcgggcctt gatctgcagg
     3781 tccgcgagtc cgtcgcgggc caggtccacg accttgcggt ccgacaccca ccccagcgaa
     3841 ctcaccgcct tgaggttcgc cagcgcgatg ttgtcctcga tcggggcact gactatgacg
     3901 ccctggctgc gccgctcctc ggggacaagg gcgatccccg cctccaggac gcggccgacg
     3961 ccgggggcgt gcggcaggcg ctcgccggca acggcgatgg tccctgacga gtgcttctgg
     4021 gcgccggcca gcaggcgcag cagctcgctg cgcccggagc cggccagtcc gccgatgccc
     4081 aggacctcac cggcgtaggc ctggaaggag acgtccttca cccggcgtcc gttcaggccc
     4141 tcgaccgcca gcaccacccg gtcggtggcg gtgctgcgct cggggaagag ctccccggcg
     4201 gaacggccga ccatcgtcga gatgacctcg tccaccgtgg tgtcggcgac ggccttggtg
     4261 gtgatggtct cgccgttgcg catgatggtg agccggtcgc agagctcgaa gacctcctcg
     4321 aggcggtggg agacgtagat gatcgcgact ccctcggcgc gcagccccct cagcaccgcg
     4381 aacaggtcgg tgatctccag gtcggtgagg gcggccgtcg gctcgtcgag gatcagcacc
     4441 cgggcatcgg tggcgagggc acgggcgacg gaaaccatgg tctgctgcac gggcgagagc
     4501 tgcccggcca gcttgccgag cgggatgcgc tggttcagcc ggtccagctg ctcctgggcg
     4561 cgcttgcgca gggcgcccca gctgaccagg gagccgcggc tgggcatctg ctcgccgagc
     4621 aggatgttct cggccacgct gattgtgggg atgatcgaca gttcctggta aagcgccacg
     4681 acgccggcct tgttggcggc ccggacgttc gcgaactcga gctcctcgcc gttgcgcacg
     4741 atctgcccgg cgtcgggcgt gtacagcccg gtgacgacct tgatgagcgt cgacttgccc
     4801 gcaccgtttt cgccgagcag ggcatggatc tcccccggct gcaccgacag gctcgcgccg
     4861 ttgagggcgt gcaccccgtc aaagcgcttg acgatgccgc gcgcctccag cgccgcggcc
     4921 ggctcgagca gggcggtccc ggtcgaggcg ggcttcaggg ccgtcatgcg gcacctcccc
     4981 cgtccccgaa gcggttgcag cggatgacga tcttcgggtg ctttccgctc acgtgcgcct
     5041 cgaaggccgc cggggcgtcc tcgaggtcga acaccgcctg ggtgaactcg tccagctcca
     5101 ggtgcggcag cacctgcagc gcccgcggga acgcgtacgg cgagacgaag acgccggtga
     5161 gcgtgagttc cttcaggtag aggtagtccg agaggttcag gggcatctcg aagtcgtggg
     5221 ggtacatcgc cccgtagacg acggtggccc ccttggcggc gatgtccagc agcccgcgca
     5281 ccgcgcgcgg cgatccggaa gcgtcgacga cgacgtcgta tcccaggtcc ccggtgatct
     5341 cctgtgcccg ccggtactgg tcctcggtga gcgggtcgat cacggcgtcg gcaccgtggc
     5401 gcagcgccat ctcgcggcgc tcggcgatcg gttcgatgag ggtgagcgag gtcgcgccgt
     5461 tcatcttcag cacctgcagg gccagctgtc cgatcgggcc gcctccgcag atggccacgc
     5521 ggtcgccgac cttgatgttc gtcttgtccg cgatccgcac ggcgacggag gccggttcga
     5581 gcaggcagcc cttcagcagg gacacgtcct cgggcagctt gtagacctgc gactcgtgcc
     5641 aggtcaccgt ttccgccatg ccgggacggt tgtactcctg gatgtgttcg cagaactgct
     5701 gctggccggt gcggcagggc gtgcacgtgc cgcagaagcg caggaagttg cccgcgacgc
     5761 ggtcgccgac gcgcaatccg ttgcggtgcg ccttctcgcc gagcgcctcg acgacgcccg
     5821 acagttcgtg gccgagcccg atcggcacct ccgtgccgaa gaacccctcc gccaggtgcg
     5881 ggtccgatcc gcagattgcg gaataggcga cccggatccg gacgtcctcc ggtccgagcg
     5941 gcggctcggc gaagtcgacc aggccgatgc ggccgcgggc ctgctcgtcg ggatcgcgga
     6001 ggctcccgat cttggtgaca gctactgttt tcattcgtct ctccggtggg cgcggaacgg
     6061 gtggaggggt caggcctgcg cggcgatgac gcgtcgggtg gcggcgatgg tcgcatcgag
     6121 cccgtagccg tgcttgttgc gcagctcctc gtcgccgccg aagcccgcgt agacctgcgg
     6181 gataccgagc cgctcgaacg cggtgaggtt gatgttgcgg tccgcgacga cgtcggcgac
     6241 ccgcgaggcg aggcccccgt acgcgaggtg gtcctccagg acgacgaacc gcccgccggt
     6301 ttcggcggcg cagcgcacga tgagctcctt gtcgatcggc ttgagcgtga acatgtcgac
     6361 gacgcgcacc gaacgcccgt ccttcgccag ttcctcggcg gcatccagtg cctgggcgac
     6421 caccgtgccg tgggtgaaga tggtggcgtc ggtgccctcg cgcgcaacga tgcccttgcc
     6481 gatgcgcacc tcgtgctgcc cgggttcgta gagcaccctg tcgctcttgc cgacggcgag
     6541 gcggatgtag atcggcccct gcatggtcat cgactggcgg atgacctcgc cgaccatcag
     6601 cgggtcgccg atggaggtga cggtcatgtt ggtcagggcg cacatgagcg cgaggtcctc
     6661 gaccgcgtag tgggtggagc cgccgttgcc gaccaggccg ccgtgggtgc cgatgatctt
     6721 caccgggagg ttcggatagc agacgtcggt gcgcacctgc tcgagcgcac gcatgctcag
     6781 gaacggaagc atgcccgaga cgaccgggat gttgccgtcg tacgccaggc ccgcggagat
     6841 gccgacgcac gcctgctcgg ccaggcccac gtccaggaac cgctccggga acttgtcgcg
     6901 gaactccacg agcgtcgctc cgatgtcggg agtgacgacc cacaggttgt cgtactcgtc
     6961 gccgagctcg gcgagggtcg atccgatgac ggaccgcgcg gagagcatct ctccaaaggt
     7021 gaacgtaacg ggcatcagac tgactccttc tcgcgcgatg cttcgagcgc cgccatcgcg
     7081 cgctccaggt cggccggccc cagcgagccg gcgtgccagg ccaggttgcg ttccatgaaa
     7141 tcgacgccct tgcccttgac ggtttcgcag atgatcacgg tcgggcggtt gctgtccgcc
     7201 gggggcagcg cgtcgagcgc ctcgaccagc tggcgcatgt cgtgcccgtc gacgttcacc
     7261 acgttccagc cgaacgccgc ccacttgtcc gggtacggct cgaagacgat gcgttcctcc
     7321 gcgaaactgg tcatcagctg gcggttgcgg tcgataacgg cgaccaggtt gccgagctcg
     7381 ttgctgcggg cggccatcgc cgcctcccag acggaccctt cgccggtctc gccgtcgccg
     7441 agcaggcaga acacgcggtg cgtttccccg cggccccggg cgctcagcgc catgcccacc
     7501 gccaggggca ggccgtgccc cagcgacccg gtggagcatt ccactccggg cagctgcacc
     7561 ttgcacgggt gcatgccata ggcggaatcg agctggccgt acgtctcgac gatcccgtcg
     7621 taggagaaga acccgcgcat ggccatcgcg atgtacatgc acaccgcggc gtgccccttg
     7681 ctcagcacga agcggtcccg cgcggggttg gcgatgtcgg cgggatcgac gttcagcccg
     7741 taatggtaaa gcgccgtgaa gatgtccgcg gcggacaggt cgccgccaat gtgcacggct
     7801 ccctcgtagg tgccgcacaa gtgcagcagc ttcgatctca gctcgaatgc gagatcttcc
     7861 aattcctcaa tcgtgggcgg gttggcaccc gtccgcaggc tcatttggcc catctcatgc
     7921 ctcctcgtcg aatgacacgg atgccgattt gccgccgttt cgtggcgtcc ccatcgactc
     7981 gttcgtggtc tgagcaccgc tgcttccggg ctcaatgtga cgaggctaac accatcctat
     8041 ttacatgtaa acaagattcc ttataaactt gtttcaccag caccgcaggg gcactcagcc
     8101 gggatgcagg cccgaaagag gccacccgcc atcgcatccc tgggctccgc gggaatgcgt
     8161 ggaccgcaaa accggcccga gagccgcatt cctctgtgac acttatctat agaccagcta
     8221 cggcgatccg gattcatgac cgccagtaag gaggggccat gactggcgag agagaccagc
     8281 ggccgcgtgc gccgataccc cctgaggccg tcacggagcc cctggtcgag acgatcgacc
     8341 cggagaactt cacgccaagg ctgctggcgc tgctgtccaa cgccctcgtg tggcgggagt
     8401 cccaggaact gcgccggcgc tacggcctcg gaacgaacga ttggcgggtc atctcggcgg
     8461 cggcaatccg tccgggcacc tcggcgaccg agttgtcgga gttcctcggc ctgaacaagg
     8521 cggtcatttc caaaagcgtc aataccctga tcgaccgcgg ccttttgctg ctgatcgacg
     8581 ggccccgcgg ctcgcggccc atgtacctga cccgggcggg cgcccagatg cacgacgcca
     8641 tgctgccgat ctcgatgcgc gggcaggaaa tcatcctggc ggacctctcc gaggaggaca
     8701 tcgaccggct caaccacctg ctgcgccgca tgctggagaa gacccgggag ctgcagatgc
     8761 tggagaacga ggcgcacgtc tgaaacaccg aacggccgcc gcggccaccc cgagggaggg
     8821 gtgtcgcggc ggccgttggg ccctgcgggt ggctgcggca ggcagcacga cggcggcgcc
     8881 gcaccttccg cggaaggtgc ggcgccgccg tcgttgcgtt tgggaagtcc ctggaggccg
     8941 gttttggctt gggaggctag ccctgggtgg tccagagcag gcgccagtcc gggtcgggaa
     9001 ggccgacgcg ggcgccgggg aagccgccga cgtccttgcc gacgtaccag ccgttcttgt
     9061 gcgccaccag cggggcgatc ggcagccact cggcgaagcg gttttgcagt tccgcgtaaa
     9121 tctccgcctt gcgttcctgg cccttgggcg tgcgcagctc atccagcagc gcgtccacct
     9181 gcgggtcccc ggccttgccg ttgttgccgg tggaggtgga gtggaagaac tggcgcagct
     9241 gctggtcgat ctcctcgcgg acctcggcga agcgcagctg ggactggtag ttcccggaga
     9301 gggcctcggt gaccatggtc ggctggtcgg acacgttcag cgtcacggtg atccccacgt
     9361 cctggagcat ctgctgcatc acctgcgcct gcttctggaa ctccggcggg gaggtggtgg
     9421 tcatcgcgaa ctcggccttg ccgccgtcgg ccacgtactc ggccaccagc ttcttggccg
     9481 cctccgggtc gaagccgggc caggaggtct ggacgtagaa cgggttcgag gacgggaaga
//
